LOCUS       DAOTKU010000001        16104 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_4056_length_16104_cov_3.674621, whole genome shotgun sequence.
ACCESSION   DAOTKU010000001 DAOTKU010000000
VERSION     DAOTKU010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 16104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16104
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4056_length_16104_cov_3.674621"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1024)
                     /locus_tag="P5310_00005"
     CDS             complement(<1..1024)
                     /locus_tag="P5310_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HRU31782.1"
                     /translation="MSIEELLKLIYERNASDLHITVGVPPILRVYGRLVRTEFPRLTP
                     EDTKSLAYSILTEKQREKLDVQGEFDFSYGIPGVGRYRINVFHQRGSVALAIRSIPFR
                     IPKLEELGLPNSLGELTKKPKGLILVTGPTGSGKSTTLASMIDIINESRDCHIVTIED
                     PIEYMHSHKKSIINQREVGSDTISFANALRAVLREDPDVILIGEMRDLETIQAAITAA
                     ETGHLVFATLHTNNASQTVERIIDVFPPHQQEQIKLQLSNTIEAIISQRLLPRKREKT
                     LSDGMIRGRAVALEILIATPAVRNLIREGKVHMIPNAIQTGSQLGMVSMDQSLLNLYK
                     AGEISWE"
     gene            complement(1021..2691)
                     /gene="gspE"
                     /locus_tag="P5310_00010"
     CDS             complement(1021..2691)
                     /gene="gspE"
                     /locus_tag="P5310_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HRU31783.1"
                     /translation="MLSQKKRLGEILVEKGIITEEILNRALSIQQGSMEKLGDVFLKM
                     GVIKEEDLYSALSDIYNVPYVNLDDVVIDSEVVRLVPEHICRRYMVIPLNIDGDRIMV
                     AMSNPVNIYALDDIRIITGKDVDPVLASERQITQVLDRIYTIQKGLEETLINIGPSDE
                     DAEATVEVLEDEAPVVRLVNLTITQAVQEGASDIHIEPTENDVKIRFRVDGVLYDVIS
                     PPKRIFPTLVARIKILSNMDIAERRVPQDGRFRVNSQGKIIDFRVSTLPTIYGEKIVL
                     RLLDRSATLIGLEKLGFTEEILHNFDNLIRRPYGMILVTGPTGSGKTTTLYSALAKIS
                     TTEKNIITIEDPVEYQFARINQVQINPKAGLTFANGLRSILRQDPDIMMVGEIRDRET
                     AEIAVQSALTGHLVLSTIHTNDSAGAIARLIDMGIEPYLISSSLIGILAQRLARRICP
                     RCRVPKEPTEEEIKVLKISSDHTMYEGKGCEACRHTGYRGRLGIFELLVIDDEIRAMI
                     VDKTPANVIREAAVKNGMVTLWEDAKLKVIDGITTYSEILRTVYTGEL"
     gene            complement(2693..3292)
                     /gene="gmk"
                     /locus_tag="P5310_00015"
     CDS             complement(2693..3292)
                     /gene="gmk"
                     /locus_tag="P5310_00015"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015721520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HRU31784.1"
                     /translation="MVEKKEGILFVVSGPSGVGKGTVINEVLKVIPDINLSVSYTTRP
                     PRKNETNGKEYFFVSEEEFDRMINDGEFLEWARVHGNFYGTPRSFIEEKLGKNKDVIL
                     EIDTQGARQVKRIFPDGVFIFILPPSIEALLTRLRGRGTDCERDIEIRLRNARDELNE
                     WEWYNYIVVNETVSFSKDLIASIILAERCRSSRLEMKGI"
     gene            3372..5000
                     /locus_tag="P5310_00020"
     CDS             3372..5000
                     /locus_tag="P5310_00020"
                     /inference="COORDINATES: protein motif:HMM:NF017633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HRU31785.1"
                     /translation="MDIFILEKIVQEIRETILDCEFRTAYSLPDNTISIEMRGRDTFY
                     LIISASSQFPRIYIDTGQSSKPGKSSFASTLNIHLNRTRLTSISQIDGERVIECIFEK
                     ETIWEENEIRKLIVEIMGKHSNIILCRDGVIIDAIKRVDSTKSRVRQVLPGLDYEYPP
                     RREIIPFSNLEDYLESFPALTVEEFLIKNIVGIGPLSIEEISARAKLDKKRVISSLDA
                     DELDALKTAIRSFREDLNRVSPVVYLDDGGNPESFYIFPLTFKRNRYEIHKSLISASA
                     RYYNWIIPHLLLENRKKSLIKELNSQIESLEKRKVELEEALSRLEDPERWKLYGDILL
                     GFNHQISPGSDKFSAEWNGEKIDIPLDPEKTPVENAQEYYERYRDEKRKKEVLPGLIS
                     EINKKIRELSKKMEEIERITSPEELEGEKPRLTKEEALPYSAYKINGYDVWVGKNAKS
                     NELITFKLSSPSDIWFHAKGYSGSHVILRTNGKRLEYIPEEVILESARLAAKHSRAKS
                     GVKVQVDYTYRKNVRSAGKKRGNVFYTNYKTIVV"
     gene            5318..5530
                     /locus_tag="P5310_00025"
     CDS             5318..5530
                     /locus_tag="P5310_00025"
                     /inference="COORDINATES: protein motif:HMM:NF021560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2283 domain-containing protein"
                     /protein_id="HRU31786.1"
                     /translation="MKIYYDDKKDLLYIRFDEKNQQIINKRVTEDIVLDIGEEDKIVG
                     MEILGASKLLNLRELLPVSYELETHS"
     gene            5558..7141
                     /locus_tag="P5310_00030"
     CDS             5558..7141
                     /locus_tag="P5310_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRU31787.1"
                     /translation="MPDNIELNEQFKRALDLLENTDKNVFITGKAGTGKSTLLNYFRS
                     ITKKKIVVLAPTGVAALNVNGETIHSFFGFKPNVTIENIKKLSPQKARIYKELETIII
                     DEISMVRADLLDCIDYFLRINVRNKPFGGIQMVFIGDLYQLPPVVTSGEKELFTKRYN
                     SQYFFDADVIRDLPLEFIELEKVYRQKDERFIQLLNEIRNNTISEESLALLNSRVGVE
                     LPESSDYIVYLTPLNATVDAINMEKLNLLKGRLYRWEAYIEGDFDKSSLPGDPILSLK
                     KGAQVMMLNNDSKGRWVNGSIGKVVGISSGKGEDEDKIEVQFPDGRIEEVIPYTWEIF
                     HYRYNEEKKLIESETIGSFTQYPLKLAWAVTIHKSQGKTFDRVIIDLERGVFAPGQVY
                     VALSRCTSFEGIVLKKPVKKGHILLDKRIVEFLTRFQYKLSERDIPLEEKIRIINQAI
                     KEKTPLSIVYLKPNDEKSRRIIQPYEVGEFSYNGKNFLGVYAYCYNRGGDRVFRVDRI
                     LEIEPAKKDAASSLPQSSQ"
     gene            complement(7147..9096)
                     /locus_tag="P5310_00035"
     CDS             complement(7147..9096)
                     /locus_tag="P5310_00035"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HRU31788.1"
                     /translation="MYLGVDYYPEHWERERWDVDARLMEDAGVELVRLAEFAWSRLEP
                     EDENYDFSWLDEVISILGGHNIKVILCTPTAAPPAWLINKHPDILPVTRNGQRMSFGA
                     RRHYCITNRNYYYYSERIIRKMAEHYKDNPQVIGWQVDNEFGGPKCYCEGCRKEFQLW
                     LKRKYGTLENLNKSWGNAFWSHDYTAWEQIPLPVVYYHNPSFDLDFRRFFSDMTVRYS
                     NMQIRLLKGLNSGKFVTHNFMGLFSELDYYDLSREYDFVSWDNYPRDSSNRYRAALAH
                     DVMRGLKKKNFWIMEEQCSYITREDITRTFPPGEMRLWTYHCYAHGAEAVVYFRWRAC
                     NFGIEQFHSGILQHDGTDNSISYRELKQIGKEMGKIGPEIENAKTVSPVAIIYDYENI
                     WVNLAYQEKPMYDYPGEILRFYRSIKRLGINVDMIKSSDDFSNYKVIIAPLLYVMTDE
                     LAKNIKDYVSGGGIFIAGFRCAIKDESSNMLKEVLPGKLRDLFGISIYAWDNIRDMAV
                     EIKDRDGKIYRGDTFADLITPEGAEVLAQYNSGWYTPYAAVTVNRYGKGKAYYTGCGL
                     SQEFYDSLMKQLLSEYSIKDIVFPEEVEVNIREKDGRRFIFITNFSDKSVSIKIKRDM
                     RDLITDKYINGETELSPFGIMILEG"
     gene            complement(9173..10231)
                     /locus_tag="P5310_00040"
     CDS             complement(9173..10231)
                     /locus_tag="P5310_00040"
                     /inference="COORDINATES: protein motif:HMM:NF014653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HRU31789.1"
                     /translation="MKELESFRVNPDLLKQFCAEVFEKLGLSDIQANITADQLVWADL
                     MGIGSHGVTRIGIYARRIQAGLVNIKPDIKPVKETSSVAVLDGDNGLGQVVGVRAVEL
                     AIEKANKYGVGVVGVRNNNHFGCAGYYALKAIENDMVGIVMSNAPSTMAPWGARVPYL
                     GTNPIAFGVPTKEFPPILLDMATSIVARGKIILSAKEGKAIPEGWAVDPEGEFTTDPE
                     LALKGAVLPFGGPKGSGIAIMIDILSGILSGAAFGPHIGSLYDDFSRPQNVGNFMIVF
                     NISDFCPVDEFKERIEKMREEIKSLPRAKGVEEIMLPGEIEFRNYMENRRSGIALSPE
                     VIKELKEVGESVSVKFSL"
     gene            complement(10747..12240)
                     /locus_tag="P5310_00045"
     CDS             complement(10747..12240)
                     /locus_tag="P5310_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HRU31790.1"
                     /translation="MRINQNIFALNAQRNLQNTNSQLSVSLERLSSGLRINRASDDAA
                     GLAISEKLRAQVSGLGVSVDNANDGISLIRTAEGSLDRVNAILKRMRDLVGWAANGDK
                     TDADRQKYQDEINQLTEEINRISTTTEYNTKKLLNGDIGSKVTVAAADAGEVESAGVS
                     GEILRTGTYVISVLSAGVAEVEGLALASGTGYSSSLYSALGASGAYTKTLNISTADGK
                     SASVTLNVASTDGDTVETAIQKINTALQNAGMVVTATWNDTNDRIELKSGEVGSKYAI
                     AVVQSNSVSGAANFTITQVTTAVDVTVSVTDPAGGANQLVKSTSRYFAAGTSTLEPVD
                     SSGIAGVTFTLTNAAAASTGAAVTLDVAKGSLILQIGPNKGEDHRMEITINDMSASTL
                     GVNNIDVTNQANAQAIIDAETIDKAIEKVVTERGKLGAYENRLNHTIQNLGVEKENLT
                     SAESRLRDADIAQEMMDFTRNQIMLQAGTAMLAQANTVPQTVLQLLR"
     gene            complement(12386..14089)
                     /locus_tag="P5310_00050"
     CDS             complement(12386..14089)
                     /locus_tag="P5310_00050"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRU31791.1"
                     /translation="MPIKIVKGKKEELDPGKLNDEGMLYLSRGMLNEAEEIFKQVIEL
                     DYNYALAHNNMAVIYLKKGLYKDAIKELRTATQLKPDYAEAFNNLGVAYFQCQKYTEA
                     KWSFEKALEIKSDYEEPRKNLAILSNNLIGSSDRSREKNVVTRNLKNKKKKEKLPAIS
                     LCMIVKNESENLPRALNSVKDYADEIIIVDTGSTDDTVEIAKSFGAKVYFYSWNDDFA
                     SARNEALRHATCDWILSMDADDEMNGEDIAKLKEYLKETDAAGLSIPIFSELTDSSLN
                     MKNTTVNYLVRIFKNDPRIRFTRRIHEFVDQSIFDMGGKIERIDIPVFHRGYTDQKTL
                     KEKLDRNKRLVEIALDENPQDHTLWTYLGKTYFTLNDLEKAEECFRKAIEIVEGKENR
                     YVTLFFLQSAYVDLALLLSEDRMEEALEFFNKAINIDPNFPDTYYYMGMAYYNKGMFE
                     EAYKSFKKVFQVDVNKSLFFISNLGIRGELTLTMLQATALQLGMLKEAIDYGNRVLEI
                     NPNNAPVLNNMGIAYISLGEREIGKSFFEKALLVAPGFEKAMDNLEKYIREEFEESIK
                     V"
     gene            complement(14160..14630)
                     /locus_tag="P5310_00055"
     CDS             complement(14160..14630)
                     /locus_tag="P5310_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HRU31792.1"
                     /translation="MSNKGLKVGDTAPDFTLNDHHNRSFKLSDFRGQKVLLSFHPLAW
                     TRVCAEQMKSLEENMSRFKELNTLPVGLSVDTVPSKHAWAKELKIRELRLLVDFWPHG
                     EVAKLFGIFREEDGFSERANIIVDEDGKIILFKIYEISQLPNIEEIISFLKDRK"
     gene            complement(14734..>16104)
                     /locus_tag="P5310_00060"
     CDS             complement(14734..>16104)
                     /locus_tag="P5310_00060"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRU31793.1"
                     /translation="NDVQIAENAYSWQLLMPMMAIVGSIGAGILIFRMNWKMALIIFS
                     LGFATFLVNLLFVKPLKIISDMIQEKLGNLTSSLSDMIAGSYVIRVFHLIDTILKRFF
                     NINYDILDLAMKRNVKQSELSAINTFLEYANFIGIIVISSIMVINKQMTFGTSIAIVQ
                     LTNPIFWLFNALGSFITQLQGSLAGARRIIELLEIPEEETGRKDLPEIQIDTSGEPVI
                     EFKDVTFWYEEEKPILKDFNLRVYRGQFIAIVGLSGSGKTTIFKLLQRFYSPQEGEIY
                     IFGKPISEYTLEEVRSLVAYVPQENYLFTGTIEENIGYGKIGATKEEIIESARYAYAY
                     DFISQLEDGFNTIVGERGAQLSGGERQRIAIARALLKDAPILLLDEATSSLDSESERQ
                     VQQAIEVLMEGRTSLVVAHRLSTIMKADIIYIISDGRIVETGSHKELMDKNGLYRYLY
                     GLQFKG"
BASE COUNT         4683 a         3667 c         2842 g         4912 t
ORIGIN      
        1 cttcccagct tatctctcca gctttataca gattaagcaa agactggtcc atactgacca
       61 tcccgagctg agaacctgtc tgtatggcgt taggaatcat atggaccttt ccctctctta
      121 tgagatttct tacagcaggg gtagctatca agatctccag agcaacagcc cttcctctta
      181 tcattccgtc gctcaatgtc ttctctctct ttctcggtaa aagcctctga gagattatag
      241 cctctatagt attactcaac tgtaacttta tctgctcttg ctgatgggga ggaaatacat
      301 ctataattct ctccaccgtc tgagacgcat tattggtatg tagggttgcg aagaccaggt
      361 gaccggtctc agctgcagtg atggctgcct gaattgtttc caggtccctc atctctccta
      421 tcaatattac atcagggtcc tctctcagca ccgccctcag tgcatttgca aaggagattg
      481 tatcgcttcc aacctccctc tgatttataa tactcttttt atgggaatgc atatattcta
      541 tagggtcttc tatagttact atatgacagt ctctactctc atttattata tctatcatag
      601 acgcaagagt agtggactta ccgcttccgg tcggaccagt taccaggatg agtcccttag
      661 gttttttggt cagttctccc aggctattgg gcaagccaag ttcttcaagt tttggaatcc
      721 tgaaagggat actccttata gctaatgcta cagaccccct ctgatgaaag acatttatcc
      781 tatacctgcc cacaccaggt atcccatagg agaagtcaaa ctctccctgt acatcaagtt
      841 tttccctctg tttttcagta aggatggaat atgccaggct cttagtatct tcaggtgtaa
      901 gtcttgggaa ctctgtcctg accagcctcc catatactct taatatagga gggaccccta
      961 cagtaatatg aaggtcagag gcgtttctct catatataag tttaagcaat tcctctatac
     1021 tcataactcc cccgtataaa ctgtcctcag aatctcacta taagtagtga taccatctat
     1081 tacctttaac tttgcatcct cccacaacgt taccataccg tttttcacgg cagcctccct
     1141 aattacattg gctggcgtct tatcaactat catagcacga atttcatcat caatcacaag
     1201 cagctcaaat atccctaatc ttcccctgta tccggtatgc ctgcaggctt cacagccctt
     1261 accctcatac atagtatggt cagaggaaat cttaagtacc ttgatctcct cctctgtcgg
     1321 ttctttaggg accctgcatc tggggcatat ccttctggct aatctctgtg caagtatacc
     1381 gataagagag gacgatataa ggtaaggttc tatccccata tctataagtc tagcgatagc
     1441 tcctgccgag tcgtttgtat gtatggtgct caacacaagg tgtccggtaa gtgcagactg
     1501 aactgctatc tcagctgtct ctctatctct tatctctcca accatcatta tgtccgggtc
     1561 ctgcctcaga atactcctta aaccattagc gaatgtgaga cctgccttgg gatttatctg
     1621 aacctgattt atccttgcaa attgatattc tacagggtct tcaatggtta ttatattctt
     1681 ctcagtagta cttatctttg ccagtgctga atatagggtg gttgtctttc cactccctgt
     1741 tggtccggtg acaagaatca taccataggg tctgcgtatg aggttatcaa agttatgcag
     1801 gatctcctct gtgaatccaa gtttttcaag cccgataaga gtggcagacc tgtccagtaa
     1861 cctgagtact atcttctctc cgtatatagt tggaagcgtt gaaaccctga aatcaattat
     1921 cttcccctga gaattcaccc tgaatcgacc atcctgtggg acacgccttt ctgctatatc
     1981 catattcgaa agtatcttga tcctggctac cagggtcgga aagattctct tgggaggaga
     2041 gattacatca tacagaacac catccactct gaatcttatc tttacatcgt tctcagtagg
     2101 ctctatatga atatcactgg ctccttcctg aacagcctga gtaattgtca gatttaccag
     2161 tctgacaaca ggcgcctcgt cctccaaaac ctccacggta gcttctgcat cttcatcgga
     2221 aggacctata tttataagcg tctcttcaag ccccttctga atggtatata tcctatccag
     2281 cacctgtgta atctgtctct cagacgctaa aacaggatcg acatctttcc cggttattat
     2341 tcttatatca tcgagggcat atatatttac aggatttgac atcgccacca taattctatc
     2401 tccatctatg ttcagaggaa tcaccatata tcttctgcat atatgctcag gtacaagtct
     2461 aacaacctca gagtcgataa ccacatcatc taggttaaca taggggacat tgtatatatc
     2521 agaaagagca gaatataggt cttcctcttt aataaccccc atctttaaaa agacatctcc
     2581 aagtttctcc atcgaacctt gttgtatgga cagggctcta ttaagaattt cttcagtaat
     2641 tatacccttt tctaccagta tctcccctaa ccttttcttc tggcttagca tactatattc
     2701 ctttcatctc cagccttgaa ctcctgcacc tctctgctaa aataatagag gcgattaaat
     2761 ccttgctaaa ggaaacagtt tcatttacca ctatataatt ataccattcc cactcattta
     2821 actcatctct ggcattcctc aatcttatct ctatatccct ttcacaatcg gttccccgac
     2881 cacgaagtct cgtcagaaga gcctctatag atggaggtaa tataaagata aataccccat
     2941 ccgggaatat cctctttacc tgtctggctc cctgagtatc tatctccaga atgacatctt
     3001 tatttttccc cagtttctcc tctatgaaag acctgggtgt cccgtaaaaa tttccatgaa
     3061 ccctagccca ctccagaaat tctccatcat ttatcatacg gtcaaactct tcttcagaga
     3121 caaagaaata ctccttaccg ttagtttcat tctttctggg gggtcttgta gtgtaggaga
     3181 ccgataaatt tatatccggg ataaccttta atacctcgtt tatcacagtt ccctttccca
     3241 ccccggaagg accagaaaca acaaatagta taccctcttt tttctcaacc ataaacaccc
     3301 tatctcttag gttttattta atttttatga tattatatca gaaagcagga tagatataaa
     3361 ggggaaagat tatggatata ttcatactgg agaagatagt ccaagagatc agagaaacca
     3421 tactggattg tgaatttaga actgcatact ctctgcctga taatacgatc agtatagaga
     3481 tgagaggaag agatactttt tatcttataa tttcggcttc aagccaattc ccaaggatat
     3541 acatagatac cggacaatcg tctaaacctg gaaagagttc atttgccagt actcttaaca
     3601 tccatttaaa caggacccga cttacatcta tctcccagat agatggagaa agagttatag
     3661 aatgtatatt tgagaaagaa actatatggg aagaaaacga gatcaggaaa cttatcgtgg
     3721 agattatggg aaaacacagc aatattattc tctgcagaga tggtgtaata atagatgcta
     3781 taaaaagagt agattcaaca aagagcaggg taaggcaggt tttaccgggg ctagattatg
     3841 agtatccacc aaggagagag attattccat tctctaacct ggaggattat ctggaatcct
     3901 ttcctgctct aacagtagaa gaatttctga taaaaaatat tgttggtata ggacctttaa
     3961 gtatagaaga gatatcagcc agggcaaaac tggataaaaa aagagttata tcttctttag
     4021 atgctgatga actagatgcc cttaaaacag ccatcaggtc ctttagagag gatttaaata
     4081 gagtctcgcc tgtggtatat ttagatgatg gaggtaatcc cgagagtttt tatatattcc
     4141 ccctgacatt taagagaaac aggtatgaaa ttcataaatc tttgatatct gcaagtgcaa
     4201 gatactataa ctggataata ccccatcttt tactggaaaa tagaaagaaa agcttaatta
     4261 aagagcttaa ctctcagata gagtctttgg agaagagaaa ggttgagtta gaagaggcac
     4321 tatccaggct ggaagacccg gagagatgga aactctacgg ggatattctg ttggggttta
     4381 accaccagat atcgccaggt tcagataaat tctcggcaga atggaacgga gaaaagatag
     4441 acatacccct ggacccggag aaaactcctg tagagaacgc tcaggagtac tatgagaggt
     4501 acagggatga aaagagaaaa aaagaggttt tacccgggct tatttcagag ataaacaaga
     4561 agataagaga actcagtaaa aagatggagg agatagaaag aattacctca cccgaggaac
     4621 tggagggaga aaaacccaga ttaactaaag aggaagcatt accatattca gcttataaga
     4681 ttaatggtta tgatgtatgg gtaggaaaga atgcaaaatc caatgagctg ataacattta
     4741 agctatcctc cccctctgac atctggtttc atgctaaggg gtatagcggt tcccatgtta
     4801 tcctgaggac taatgggaaa aggctggaat atatcccgga agaggtaatc ctggagtctg
     4861 cccgtttagc agccaagcat agcagggcaa agtcaggggt caaggtacag gtggattata
     4921 catacaggaa aaatgtaagg agtgccggga agaaaagggg caatgttttt tacacaaact
     4981 ataagacaat agtagtatga gtcgcgatgt attctttaca aacagaatac aataagggta
     5041 taattttcta gatgaagatt aaaattgagc ctcatacgtt ggagcgagca gaggagaggg
     5101 gaacaaatct aaaagaaata atagatacta ttctaacagg atttcctata aaggcaaaat
     5161 atgggcgttt atgaaaagca aaaatatatc catataatca gatgagggca aaaaagtttt
     5221 atgaacaaaa gcgtgttgaa gttatttata ctgttgaggg tgaaaatatt attacagtta
     5281 ctgtttatgt gttctatggt aaatgggaga attaaaagtg aaaatttatt atgacgataa
     5341 gaaagattta ctctatatta gatttgatga aaaaaatcag cagattatca ataaaagagt
     5401 tactgaagat atcgtccttg acattggaga agaagacaag atagtaggga tggagatttt
     5461 aggagcctca aaactcttga atctcagaga gcttctccct gtttcttatg aacttgaaac
     5521 tcatagctaa actgtctgtc gtgttataat ttataatatg ccagataaca tagaactgaa
     5581 tgaacagttt aaaagggcgt tagatctatt agagaataca gataagaatg tctttatcac
     5641 aggaaaggct gggacaggaa agtccacatt actaaattat ttccgatcca taacaaagaa
     5701 gaagattgta gtcctggcac ccacaggggt tgctgctcta aatgtaaatg gagagacaat
     5761 acactccttc tttggattta agccaaatgt aaccattgaa aacataaaga agctttctcc
     5821 ccaaaaggca aggatctata aagagttaga gacaattata attgacgaga tatcgatggt
     5881 gagggcagac ctactggatt gtatcgacta cttcctccgt ataaacgtaa ggaacaaacc
     5941 attcggcggt attcagatgg tctttatcgg agacctctat cagcttccac cggttgtaac
     6001 atcgggggag aaggaacttt tcaccaaaag atataacagc cagtacttct ttgatgccga
     6061 tgttataaga gatttaccat tagaattcat tgagctggag aaggtctaca gacagaagga
     6121 tgaaagattc atacagctcc tcaatgaaat cagaaataac actataagtg aagagagtct
     6181 ggctcttctc aatagcaggg taggggtaga attaccagaa agttcagatt atatagttta
     6241 tcttacccct ctaaatgcga ccgtagacgc aatcaatatg gagaagctta acttactgaa
     6301 gggaagactt tatagatggg aggcatatat cgagggagac ttcgataaat cctctttgcc
     6361 cggagacccc atcctttccc ttaaaaaagg tgctcaggtt atgatgctta acaatgatag
     6421 taaggggaga tgggtaaacg ggtctattgg aaaagtggtc ggtataagct ccgggaaggg
     6481 agaagatgaa gataaaatag aagttcagtt ccctgatggg aggatagaag aggtcattcc
     6541 ctatacctgg gagatattcc actacagata caatgaagag aaaaaactga tagaatcaga
     6601 aactatcggt tcatttaccc agtatcccct taaacttgcc tgggcggtta ccatacacaa
     6661 gagccagggg aagacattcg atagggttat aatcgatctg gaaagaggag tttttgcccc
     6721 gggacaggtt tatgttgccc tgagcagatg cacatctttt gagggaattg tcctcaagaa
     6781 acctgtaaaa aagggacata tactcctgga caaacgtata gtagaatttc tgaccagatt
     6841 tcagtataaa ctctcagaga gagacatccc tctggaagaa aagataagga taataaatca
     6901 ggctataaaa gagaagaccc ccctgtctat agtgtatctg aagcccaatg atgaaaaatc
     6961 gagaaggatc atacaaccat atgaagtggg agagttctcc tataatggta agaactttct
     7021 tggtgtatat gcatactgct ataacagggg aggagacagg gtcttcagag ttgacaggat
     7081 actggagata gaacctgcta aaaaggatgc tgccagctct ttaccccaat cttctcaata
     7141 gaagatctaa ccttccagta tcataatccc aaacggagat agctctgtct ccccattaat
     7201 atatttatcg gttatcagat ctctcatatc cctttttatc tttatagaga cagatttatc
     7261 actgaagttt gttataaata taaatcttct cccatctttc tctcttatat ttacttcgac
     7321 ctcttctgga aagactatgt ctttgatgga atattcagaa agtaactgtt tcatcagaga
     7381 atcatagaat tcctgggata atccgcatcc tgtataataa gcctttccct tcccatatct
     7441 atttaccgta acagcagcat atggagtata ccagccacta ttatactgag ccagcacctc
     7501 tgccccttca ggtgttatca ggtctgcaaa agtgtctccc ctgtaaattt taccgtccct
     7561 gtcttttatc tccaccgcca tatcccttat attatcccaa gcgtatatac ttataccaaa
     7621 aaggtctctc aacttacctg gcagcacttc tttcagcata ttactgctct catcctttat
     7681 ggcacacctg aatcctgcta tgaatatccc tccaccagag acgtagtcct ttatattttt
     7741 tgccagctca tctgtcatta catacagcag gggggctatt atcaccttat aattactgaa
     7801 atcatccgag gattttatca tatctacatt tataccaagc ctttttatag acctgtagaa
     7861 tctcaatatt tctcccgggt aatcgtacat aggtttttcc tgataagcca gattaaccca
     7921 tatgttttca tagtcgtata ttatagctac cggagatact gttttagcat tctcaatctc
     7981 aggtccaatc tttcccatct ctttgccaat ctgctttaat tccctgtaac ttatggaatt
     8041 gtctgtacca tcatgttgca ggataccgga gtggaactgt tctatgccaa agttacatgc
     8101 cctccacctg aagtaaacca cagcctctgc tccatgggca taacagtgat atgtccacaa
     8161 tctcatttcc ccaggaggga atgttctggt tatgtcttct cttgtgatat aactgcactg
     8221 ctcttccata atccagaagt ttttcttctt taatcccctc atcacgtcat gagccagtgc
     8281 agctcgatat ctgttactgg agtctctggg gtagttatcc caggatacaa agtcatactc
     8341 cctggacaga tcgtaataat ctaactcaga gaatagtccc ataaaattat gagttacaaa
     8401 tttacctgaa tttaaccctt taagcaacct tatctgcata ttactgtatc tgaccgtcat
     8461 atcagaaaag aatcttctaa aatcaaggtc aaacgaggga ttatgataat agacgacagg
     8521 aagaggtatc tgttcccagg ctgtataatc atgactccag aatgcattcc cccaggattt
     8581 atttaaattc tctaaagtgc cgtattttct tttcaaccag agctgaaact cttttctaca
     8641 gccctcacaa taacattttg gtccgccaaa ttcattatcc acctgccatc caataacctg
     8701 aggattgtcc ttataatgtt cagccatctt tctgattatt ctttctgaat aatagtaata
     8761 gttcctgttt gtgatacagt agtgtcttct ggctccaaaa gacatcctct gtccattcct
     8821 ggttacaggt aatatgtcag gatgtttatt aataagccag gctggtggtg ctgctgttgg
     8881 tgtacacagt ataaccttta tgttatgccc gccaagtatt gagattacct catctaacca
     8941 tgaaaagtcg taattttcgt cctctggctc taacctgctc cacgcaaatt cagccaatcg
     9001 aactaactct acgccagcat cctccataag ccttgcatca acatcccatc tctctctttc
     9061 ccagtgttca ggataataat ctacaccaag atacatgata gaaaacctcc tcttctataa
     9121 attaatattt ttagccacag gaaaacccat catcacaatc ttatcgctct ccttaaagag
     9181 aaaacttgac tgaaaccgat tccccaacct ctttaagttc cttaatcacc tcagggctca
     9241 gagctattcc agaccttcta ttctccatat aatttctgaa ctctatctcc cctggtagca
     9301 ttatctcctc tacaccttta gctctaggta aactctttat ctcctctctc atcttttcta
     9361 ttctctcttt aaattcatcg accggacaaa aatcagagat attaaatact atcataaagt
     9421 tccctacatt ttgtggtcta gaaaagtcat catacagaga gcctatatgt ggaccaaacg
     9481 cagcaccact caagatacca ctgagtatat ctatcataat ggctatccca gaacccttag
     9541 gacctccaaa gggtaacaca gctcctttta atgctagctc cgggtctgta gtaaattctc
     9601 cttccggatc aacagcccaa ccttctggaa tagccttccc ttccttagct gatagaataa
     9661 tcttccctct ggcaacaata ctggtagcca tatccagaag tattggggga aattctttag
     9721 ttggaactcc aaaagctatc ggatttgtcc caagataagg aactctggca ccccagggag
     9781 ccatagtaga tggagcattt gacataacta tcccaaccat atcattctct atagccttta
     9841 atgcatagta accagcacac ccaaaatgat tattatttct cactccaaca actccaaccc
     9901 catatttatt agccttttct atagctaact ccactgccct gaccccaact acctgaccca
     9961 acccattgtc cccatctaaa actgctactg atgatgtctc cttaacaggc ttgatgtcag
    10021 gctttatatt cactaatcct gcctgaattc ttctggcata tatgccgata cgtgttacac
    10081 catgagaacc tatccccata aggtcagccc agaccagctg gtcggcagtt atattagcct
    10141 gaatatctga aagaccaagt ttttcgaaaa cttctgcaca gaattgtttt aataaatctg
    10201 gatttactct aaaagattcc agttctttca ttttcctatc aatccctctc tttccagaac
    10261 ctgccttaat gaaagtctat cggattctcc tctccaggag gagatcttac attacagtgt
    10321 catctaaggg caatccattc cttactttta cagtgaaagc cttcaaaaga ttacagggaa
    10381 aatactgtcc catccagaaa cgatgtctcc ctgtgattaa tctttctata agattgtatt
    10441 gtttaatatt attattgtca actgagtgtt aacgttctgt aatattgtta tgggtaaata
    10501 gttctgacct attcccctcc cccttgacgg gagaggaagg ataaaggcat ggtgttttta
    10561 ttataaaaga cgaaattgta ggggcaattc atgaattgcc cctacgggat acaactcata
    10621 tgtttcattt cctccccctg acattttcac agactattga cgctgaatac gctgaagata
    10681 gttctttaaa atataacagg gcaggcttga gcctgccctg tagagaaaag tttccagaaa
    10741 accttattat cttaagagct gaaggaccgt ctgaggtact gtatttgcct gagccagcat
    10801 tgcagtgcca gcctggagca taatctggtt cctcgtgaaa tccatcatct cttgtgctat
    10861 atctgcatct ctcagcctgg actctgcaga tgtcaggttc tctttttcta ctccaaggtt
    10921 ctggattgta tgattcaatc tattttcata agcaccaagt ttaccacgtt cagtaacaac
    10981 cttctctata gccttatcaa tagtctcagc atctataata gcctgggcat ttgcctgatt
    11041 cgttacatct atattattca ctcccagtgt agaagcactc atatcattta tggtgatttc
    11101 catcctgtgg tcctcaccct tgttaggtcc aatctgaagt atgagagaac ctttagcaac
    11161 atctagagtt accgccgcac cggtagaggc tgctgcagca ttggttaatg taaatgttac
    11221 ccctgctata ccacttgaat cgacgggttc aagtgttgat gttccagcgg caaagtatct
    11281 gctggtggat ttcaccaact gattagcccc acctgcaggg tcagtaacac ttacagtcac
    11341 atctacagct gttgttactt gagttattgt aaagttagct gctccagata cactattgct
    11401 ctggactacc gctatggcat acttgcttcc gacttcacct gatttaagct caattctatc
    11461 attggtatca ttccaggtag cagtaactac cattcctgca ttctgtagag ctgtatttat
    11521 cttctggata gcagtctcta ccgtatctcc atcggtactg gctacattta atgttacgct
    11581 tgcactctta ccatcagcgg tagagatatt cagggtcttg gtataagctc ctgatgcacc
    11641 cagagcacta taaagactgg agctataacc agttccgcta gccagtgcaa gaccttctac
    11701 ctctgccact ccagctgata aaactgaaat tacataggta cctgttctga gtatctctcc
    11761 acttacacct gcagactcca cttctcctgc atctgcagcg gctaccgtca ccttcgagcc
    11821 aatgtctcca tttaatagtt tctttgtgtt gtactctgtc gtggtcgaga ttctgtttat
    11881 ctcttctgta agctgattta tctcatcctg atacttctgt ctatccgcat ccgtcttatc
    11941 accgttagcc gcccagccta ccaggtctct catcctcttg aggatagcat tcaccctatc
    12001 gagtgaccct tcagctgttc ttataagaga gataccatca ttggcattgt ccacagatac
    12061 tccaagccca cttacctggg ctctcaattt ctcggagatt gcaagcccag ccgcatcatc
    12121 agatgccctg tttatcctca aaccagagga caatctctcc agagataccg ataactggct
    12181 attagtattc tgcagattcc tctgcgcatt caacgcaaaa atattctgat taatacgcat
    12241 cttctatacc tccttgttta aaatatttta ttggcttcca tgcctctaaa ctcacctcta
    12301 ctccccttaa atcaagtgtc ccatcaactc tttacactac tggacctgtc tatcacctcc
    12361 aacacatata atcggaactt ccattctaaa ctttaattga ttcttcaaac tcttccctga
    12421 tatatttctc cagattatcc atagcctttt caaacccggg tgctaccagt aaagccttct
    12481 caaaaaagct tttcccaatc tccctctccc caagtgatat gtatgctata cccatattat
    12541 taagaacagg agcattattt gggtttatct ccaaaaccct atttccataa tctatagcct
    12601 ctttaagcat ccctaactgc agggctgtag cctgtagcat cgtaagagta agttctcctc
    12661 taataccaag attggatatg aagaatagac tcttattcac atctacctga aagaccttct
    12721 taaaagattt ataggcttcc tcaaacatac ctttattata gtaagccatg cccatatagt
    12781 aataggtatc cgggaaatta gggtctatat ttatagcctt attaaaaaat tctagggctt
    12841 cttccattct atcttcagac aacagaagag ccagatctac atatgcactc tggaggaaga
    12901 aaagggtcac atacctgttc tcctttcctt caacaatctc aatagccttc ctgaaacatt
    12961 cttcagcttt ttccaaatca tttaaagtaa aatatgtctt accaagataa gtccagaggg
    13021 tatggtcctg agggttttca tccagagcta tttctacaag tcgcttattc ctgtctaact
    13081 tctctttaag agtcttctgg tcggtataac ctctatgaaa aacagggata tcaattcttt
    13141 ctatctttcc acccatatca aagatagact ggtctacaaa ctcgtggatt ctccgtgtaa
    13201 atcttatcct ggggtcattt ttaaatatcc ttacaaggta gttcacagtg gtattcttca
    13261 tattcagaga actatcagtt aattcgctga atatggggat agaaagacct gccgcatctg
    13321 tctcttttaa gtactctttg agcttagcaa tatcttcccc attcatctca tcatctgcat
    13381 ccattgataa gatccaatca caggtagcat gtctcaaagc ctcattcctt gcagaggcaa
    13441 aatcatcgtt ccagctgtaa aaatatacct tcgcaccaaa tgatttggct atctcaactg
    13501 tatcatctgt tgaacccgta tctactataa tgatctcatc cgcataatct ttcacagaat
    13561 ttaatgcccg gggaagattc tctgattcat tctttacaat catacagaga cttatagctg
    13621 gaagcttctc tttcttcttt ttgtttttga gattcctggt aactacattc ttttccctgg
    13681 acctgtctga actgcctata agattattag ataaaattgc aagattcttt cttggttctt
    13741 catagtctga ctttatctct aaagccttct caaaagacca tttagcttcg gtatactttt
    13801 gacattgaaa atatgccact cctaaattat taaaagcctc ggcataatct ggcttcaatt
    13861 gagtagcagt tcgaagttcc tttatagcgt ccttataaag tccttttttc agataaataa
    13921 ccgccatatt attatgcgcc aaggcatagt tataatctaa ttctattacc tgcttaaaga
    13981 tctcttcagc ttcatttaac atccctctgc tcaggtagag catcccttca tcattcagtt
    14041 tacccgggtc taactcttct ttcttcccct ttactatctt tataggcact ttctatcacc
    14101 ttcctctaac aggttaattc ggaaagacag gggaaaactt taacagggta tcagaggttt
    14161 cattttctat ctttcagaaa acttataatc tcttctatat taggcagctg agatatctcg
    14221 tatatcttaa ataatataat cttcccatct tcatcaacta tgatgttagc cctctccgag
    14281 aatccatctt cttctctgaa tataccaaaa agctttgcca cctctccgtg tggccagaaa
    14341 tctactaaaa gtctcaattc ccttatcttc aactctttag cccaggcatg ttttgacggg
    14401 acagtatcca cacttaaacc cacgggaaga gtgttaagtt ctttaaatct actcatattc
    14461 tcttccagag atttcatctg ttctgcacag accctcgtcc aggcaagtgg atggaatgac
    14521 aggagaactt tttgacctct gaaatctgaa agcttaaaac tgcgattgtg atggtcattt
    14581 aaagtaaaat caggggctgt atcccctacc ttaagacctt tattgctcat agttatccct
    14641 ccttgtctat accttcagaa atattatctg aaaagaatta taacataaaa attattcaga
    14701 atatccagaa atgaacaaag taaatatttg ccatcaaccc ttaaattgca acccgtagag
    14761 atatctgtat agaccattct tatccataag ctctttatga cttcctgttt caactattct
    14821 cccgtcagaa ataatataaa ttatatccgc cttcattata gttgatagtc tgtgagctac
    14881 tactagagat gtccttccct ccatcaatac ttcgattgcc tgctggacct gcctttctga
    14941 ttctgaatcc aaggatgatg ttgcctcatc caggagtaat atcggggcat ctttgagaag
    15001 agctctggct atcgctatcc tctgcctttc accacctgag agctgagccc ccctttctcc
    15061 cactatggtg ttaaatccat cttctagctg ggagataaaa tcataggcat atgcatatct
    15121 tgccgattct ataatctctt cttttgttgc tcctatctta ccataaccta tattctcctc
    15181 tatagttccg gtgaagagat agttctcctg aggaacatat gccaccaggc ttcttacctc
    15241 ttctaaggta tactcggata tgggcttacc aaatatatag atctcccctt cttgaggaga
    15301 atagaacctc tggagtagct taaagattgt tgtcttacca ctgccactta gacctactat
    15361 agctataaac tgtcccctgt ataccctgag attaaaatct ttcagtatag gcttttcctc
    15421 ttcgtaccag aatgttacat ctttgaattc aataactggt tcaccagatg tatctatctg
    15481 tatctcgggt agatccttac gaccggtctc ttcttccggt atctccagta gctctatgat
    15541 gcgtcttgcc ccagcgagag aaccctggag ctgggttata aagctaccca gggcgttaaa
    15601 cagccagaat atgggattag taagctgaac tatagcgata cttgtcccaa acgtcatctg
    15661 tttatttata accattatac tggagataac gattatacct atgaaattgg catattccaa
    15721 aaatgtgttg atcgcactca actctgactg cttaacattc ctcttcatag ccaaatccag
    15781 gatgtcataa tttatattaa agaacctttt taatatcgta tctataaggt ggaataccct
    15841 tataacatag gaacctgcaa tcatatctga cagactactg gtaaggtttc ccagcttttc
    15901 ctgtatcata tcacttataa tcttaagtgg ttttacaaac aggaggttta ccaggaatgt
    15961 ggcaaagccc agagagaata taatcaaagc catcttccag ttcattctga atatcaggat
    16021 gcctgcccct atgctaccaa ctatagccat cataggcata agtaactgcc agctataggc
    16081 attctctgct atctgtacat cgtt
//
LOCUS       DAOTKU010000002        13995 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_4793_length_13995_cov_3.529412, whole genome shotgun sequence.
ACCESSION   DAOTKU010000002 DAOTKU010000000
VERSION     DAOTKU010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 13995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13995
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4793_length_13995_cov_3.529412"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2108)
                     /locus_tag="P5310_00065"
     CDS             complement(<1..2108)
                     /locus_tag="P5310_00065"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRU31794.1"
                     /translation="MEDFKDYINPYIKRELRILKLDKNFTYGEGNYLYDNVGNRYLDF
                     ISSFGAVPFGFNYPEIWEVLEEIKGEKLPSIVQASISESQESVAEKLISLAPEGLKYV
                     VFTNSGTEAVNIAIKMARLSSGKRNIIVAEGGYHGMQEDGFKYVPYGDIDVLEEELKN
                     GGARYAGVMMEPIQGEGGIVIPPKGYLTQVSELCKKYRVVFIIDEIQTGLGRTGFLFA
                     CEEEGITPDILLIGKVLGGGIYPVNACLVKEEFYPRGFDLNYFSTFAGNNIACKITDR
                     VIDIITRNNRELIYRIRENGERLLNELLKLRDDFPKLLKDARGKGYFLGLELEMKREY
                     YPQSMLSILSEQNSLSYPIVSYLLNEKKIRLAPTLRNSKVIRLEPSFITEWKDCEEVI
                     NSFRDVLSILNEGNTPELLRPILDLRREELSNYTIPSFERESWDNSLMPSPDEEEGRF
                     AFLVHPLDLANYYEFDPTLSALPEEKLELFSQISKEIIRPFVIGKVKITSQCGARAYG
                     EFVNIPYTARQMIETPQDEMLETIEDAIKLAVDRGARIVGLGAYTSVVTRGGTLLKGK
                     YVPLTTGNSYTVVAGVDAIKFASEKSGIPLSEATASIVGATGAIGKALAILLGKDLHR
                     IILVGNPAHPKSSVRRLRRVGIELCIYISKLLNTGWNPYEGSIGEYLSGLRLPSPDDL
                     DSWNGILEVLKENGFISITT"
     gene            2196..3089
                     /locus_tag="P5310_00070"
     CDS             2196..3089
                     /locus_tag="P5310_00070"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRU31795.1"
                     /translation="METLVSVIIPTRNRRTIIGKCIECILNQTYRSYEVIVIDDGSED
                     GTEDTLDKFSSNDNFKYIKLGERKGPAYCRNLGVRESNGKILIFIDSDIFVLPDFIES
                     HIETHRNHPEGIVGVGPVIAISKIDEKLPEKGSVLDFSNAYFASGNASLSRDIYLKVR
                     GFDEVFNVYGWEDIDFGLKLKSLGIKSVKVPRAIGYHYQPLPKEEDIDSLIEKERERA
                     RTALYLYKKYPTLEVKLMIQLSSFHRILNRFLTGFGYIDKKRVIRILNSNRSYNIKRL
                     VLTVFLTRVYLDALEKELKVL"
     gene            complement(3129..3629)
                     /locus_tag="P5310_00075"
     CDS             complement(3129..3629)
                     /locus_tag="P5310_00075"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRU31796.1"
                     /translation="MEKRNLPKWLPYLVVAVVVIAIIIAVSTGGGPEEQEVTTETAEE
                     VTEEFVGTEETTETEEVAEETEAAAEMAPDEAEYNRIRDLYYEGEYEKAIEEYRAFLE
                     QYPDSEWADDAQYDIASSYEYLEKYEEAINEYQKLIDEYPDSDLAESAQWSIDYLKSY
                     EEEGTE"
     gene            3793..4275
                     /locus_tag="P5310_00080"
     CDS             3793..4275
                     /locus_tag="P5310_00080"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HRU31797.1"
                     /translation="MNKNYSIEEILEIAVKIEENGVLFYRTLAEQTRDKRIEELFRFL
                     EGEEREHISVFKNIYENLTKKTFEKAFSEEEANMYLHALIENRIFKKPEEIISTVSRN
                     DGLSAVDIAIQVEKDTILYYYEILENLTGKEKDLVKELINQEKTHVYRLTTLKNTLRG
                     "
     gene            complement(4297..4950)
                     /gene="fsa"
                     /locus_tag="P5310_00085"
     CDS             complement(4297..4950)
                     /gene="fsa"
                     /locus_tag="P5310_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012513858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HRU31798.1"
                     /translation="MKFFADTANIEEIRQIKKWGLLDGVTTNPTIISRENRPFKEVVM
                     EILNTVDGPVNLEVISTEADGMIKEAIKLSSLAPNVVVKIPMTPEGIMAVKELSNRGV
                     KTNVTLTFSPTQALIAAKAGAGYVSPFIGRWDDISTEGMQVVEDILTIFNNYGYKTEV
                     IVASVRHPIHILQAARLGAHIVTAPFKVLEQLFHHPLTDIGLKRFLEDWKKVPNPEI"
     gene            complement(4955..5653)
                     /locus_tag="P5310_00090"
     CDS             complement(4955..5653)
                     /locus_tag="P5310_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRU31799.1"
                     /translation="MLKIEGIDVYYGVIRALKEVSLTVNDGEIVTLIGANGAGKTTTL
                     RTISGLIRPKKGTITFDGQDLTRMAPNEIAGLGILQVPEGRRVFSNLTVMENLLMGAY
                     LRRDDISRELDWVFSLFPRLKERQKQKAGTLSGGEQQMLAISRALMGKPRVILLDEPS
                     LGLAPLLVLNIFETIKEINGQGVTILLVEQNAYMALQIAHRAYVIETGRVVMEGTGKE
                     LLNNDAVKKAYLGG"
     gene            complement(5654..6964)
                     /locus_tag="P5310_00095"
     CDS             complement(5654..6964)
                     /locus_tag="P5310_00095"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HRU31800.1"
                     /translation="MYGEILMHVLEKARRIDTEVLLVHTNRTLTRFANSYIHQNVSVD
                     DIDLSIRVIVDNRTGKVSINQLSTDALDEALERAISIAKLTPPDPDYYGLPRPLPIRS
                     KKDTVQKETDPEKLAQKVIDFIEATDGLNASGALELTDTTVAIVNSNGISVEDSVSMC
                     SLEAVVQDGDSSGYVYDMEKDLDSIDFISLAKIASNKARNGQNPVELSPGYYTVILEP
                     QAAGTLVSFLGLMGFGAKQFEEGRSFLVGKLSEKIASELINIWDYPSHSKTISLPFDF
                     EGLPRQDVILIEGGIARNLVYDFKYAKKEGKTSTGNALPQPSTDGPLPFNLTLLPGDN
                     TLEDMIASTKKGILVTRFHYTNMVDPVKTIITGMTRDGTFLIEDGEITRPVKNMRFTQ
                     SILEALNSTEAVGKDLHIAGEYFIYPTLVPALKLGNFHFTGVTR"
     gene            complement(6965..8404)
                     /locus_tag="P5310_00100"
     CDS             complement(6965..8404)
                     /locus_tag="P5310_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HRU31801.1"
                     /translation="MKDIAHEALDIAMGSGASFCEVRVLRRRYQGINVKNESVESLID
                     HTEEGIGIRVIVNGAWGFAGTSDLQRDKILESAKRAIQVAKASSLAKKEDVILSPAPK
                     IIATWSNNVKVNPFDVSLDKKVSLLIESAKIMGKIRDVKIAEGSMDFWETEKLFVNSE
                     GTEILQHIIESGGGISAYAIGEGELQRRSYPNSFGGQYETRGYELIEEMDLLNNAQRV
                     AEEASALLDAPQCPSITTTLILDGSQVALQVHESIGHPIELDRILGMEASYAGTSFIS
                     IGDRGKLKYGSKIMNVSADATLPGGLGSFGYDDEGVPAQRIEVIREGVLQNFLTNRET
                     AYILGETSNGTCRANGWGNIPLIRMTNINLEPGGGTLEDLIRDTEDGIFMSTNKSWSI
                     DDRRLNFQFGLEIAWEIKNGKLMGILKNPNYTGITPEFWSSLDAVCGPGEWKIWGVPN
                     CGKGQPGQIAHVGHGTAPARFRNVRVGVR"
     gene            complement(8420..9190)
                     /locus_tag="P5310_00105"
     CDS             complement(8420..9190)
                     /locus_tag="P5310_00105"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRU31802.1"
                     /translation="MEIPVVFGKEGHRLVGILHSVDDGERKPGVVLFHGFTGNKIEPH
                     RLFVKIARKLASIGINVLRFDFYGSGDSEGEFIEMTFSGEVEDALVAVDFLREQECVD
                     PSRIGVLGLSMGGGIASYVSGKREDIKSTVLLSAVAELSSLAEGFRTQYPEDKLRAEG
                     FVDYGGWQLGLGFIGELPDIQPVEMIKNFKGPVFIIHGSGDNVVPVAAAYKYYEALKG
                     RAVETRLKVIDGADHTFNSVPWEKEVLGLVSDWFIKTL"
     gene            complement(9327..10637)
                     /gene="hcp"
                     /locus_tag="P5310_00110"
     CDS             complement(9327..10637)
                     /gene="hcp"
                     /locus_tag="P5310_00110"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HRU31803.1"
                     /translation="MNSAEKLDMFCYQCSWTTNGTGCTVKGVCGKVPTVARLQDNLLF
                     SIKGITAYLYHARELGYTDPEVDAFLESAFYSTLTNVNFDPEKFVALALETGRMNIKT
                     MRLLKTAHIEKYGEPVPTRVRTGSVKGKGIIATGHGLKALEELLKQTEGTGINVYTHS
                     ELLPAHGYPGLKKYSHLVGNLGKAWHDQRQLFNKYPAAILGTSNCVLPPLEGYKDRMF
                     TTGVTGLPGVKHIDGYDFTPVIEKAKSLPDLEDEHKEEFLVTGFSKSVILSLAEKIKS
                     LVEAGKIRRFFLVGGCDAPLKKSQYYREFVEKLPKETIILTLACGKFRINDLDLGEID
                     GVPRLIDIGQCNDAIVAIEIAQALSELFGVGVNDLPLTLVLSWMEQKAVAILWSLLAL
                     GIKSIYIGPILPAWVNDDILNVLRSEYDLRLITEPEEDIKNIFS"
     gene            complement(10801..10876)
                     /locus_tag="P5310_00115"
     tRNA            complement(10801..10876)
                     /locus_tag="P5310_00115"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10841..10843),aa:Asn,seq:gtt)
     gene            complement(10922..12049)
                     /locus_tag="P5310_00120"
     CDS             complement(10922..12049)
                     /locus_tag="P5310_00120"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRU31804.1"
                     /translation="MTSRERFVETLTFGKPDKIPLMPGGPRESTLKRWREEGLPEGKN
                     YYSALLEILGIEKEKEEERIDLGVSFKMIPQFEEKIIEHRDGHYIVQDWMGAIVEISD
                     QYDYTYLREAKDFVTRKWYKFPVEDYSDWEEMKKRYDPNSPGRFPEDFEDRCKRVQDR
                     SYVLSLSFDGPFWQLREWCGLENLCIFMIERPDFVKEMIDFWAGFVLEVLRRIISRVQ
                     LDYVLISEDMAYKEHSMISPGMVREFLFPAYIRWIPELRKSGCPIISMDSDGYIGELI
                     PLWIEAGFNCCTPVEVAAGNDIVEFRRRFGKSMAYIGGIDKRAIAKGGKEMEREVLRV
                     VPPLLKDGGYIPSCDHGVPPDISWRNFLDYTRLLAKLTGWI"
     gene            complement(12049..13266)
                     /locus_tag="P5310_00125"
     CDS             complement(12049..13266)
                     /locus_tag="P5310_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012818761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRU31805.1"
                     /translation="MKRYVMVGASSRGFYMYAIPITERFKDYAKLVGIFDINRKRAEY
                     VSNYCGGIPVFDDFDKMLEETHPDCVIVTTIDRYHHEYAIRAMKAGCDVIVEKPMTID
                     DEKCRAMLETEKKTGKRIIVTFNYRFNPYVTRIKELLKEKVVGEILNVDFEWFLDTRH
                     GADYFRRWHRRKENSGGLLVHKATHHFDIINWWIEEDPEVVYANGTRRFYGPTRENRG
                     ARCLTCQYKDSCEFFVDYSKDPFMKVFYFDAEDIDGYYRDGCVFSPEIDIEDTMSVTV
                     RYSKGALLTYSLVAYSPYEGWRMSITGTDGRIEAEEFHSGHYVREPDQYIKLFNRKDE
                     IVTYEIPKVTGGHGGSDERLQNMLFTKNQPDPLGHMADSRAGAMSIMIGISANKSIAT
                     GNPIRVRDLLEVI"
     gene            complement(13279..>13995)
                     /locus_tag="P5310_00130"
     CDS             complement(13279..>13995)
                     /locus_tag="P5310_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU31806.1"
                     /translation="DKRPLYPRTIESGSFNGFPYKKVFMFTETDITTAGVYFSGEGSN
                     LCTLLLLDNGTEDVLKEFDLIRSFLSRGDVFVYDPRGIGSVKCRDINLHGFYEYYGTE
                     YKLNFDLIMLGTSMTALRVFDILRAFEYVKEVNPKATLSIAGKGVSSIYALFASVIDE
                     SVKEIYLEDILYSYEELISSRYYRYDPRLEIYGIARYFDIKDLISCISDRNVVLVNPR
                     DSRGEIANIEELGGVKAYRY"
BASE COUNT         4058 a         3338 c         2230 g         4369 t
ORIGIN      
        1 tctgttgtta ttgatataaa accgttctct tttagtactt ctaatatacc attccagctg
       61 tctaaatcat caggagatgg taatcttaga ccagacaggt attcccctat acttccctcg
      121 taaggattcc atccggtatt caacagtttt gatatataga tgcagagctc tataccaacc
      181 ctcctcaacc ttcttacact ggacttagga tgagccggat ttccaacaag aattattcta
      241 tgcagatctt taccaagaag tattgccagc gccttaccta tagcaccagt agcccctaca
      301 atgctggcag tagcttcact gagaggaatt cctgactttt cagaggcaaa ttttatagca
      361 tccaccccag ccaccaccgt ataactattt cctgtagtta acgggacata cttacctttt
      421 aacagtgttc cccctctggt gactaccgag gtatacgccc ccaatcctac tattcttgcc
      481 cctctgtcaa ccgcaagttt tatcgcatct tctatggtct caagcatttc atcttgaggg
      541 gtttctatca tctgacgtgc tgtatagggt atattgacaa actccccgta agctctggct
      601 ccacattgag aggttatctt aacctttcct ataacaaaag gtctaatgat ttctttactt
      661 atctgagaaa agagttccaa cttttcttcg ggaagagcag agagtgtggg gtcaaattca
      721 taataatttg ccagatctaa ggggtgtacc agaaatgcaa aacgaccctc ttcctcatct
      781 ggagatggca taaggctatt atcccacgat tctctctcga aagaaggtat ggtataatta
      841 gataactctt ctctccttaa atccagaatc gggcgaagaa gttcaggagt attcccttca
      901 tttaatatac ttaagacatc cctgaaagaa tttataacct cttcgcaatc cttccattca
      961 gttataaaag atggttccaa tctaattacc ttactatttc ttaaagttgg agccagtcta
     1021 atttttttct cgtttaataa gtaactgacg attggataag agaggctatt ctgctctgaa
     1081 agtatactta acatactctg aggataatat tctcttttca tctccaattc caaccccaaa
     1141 aagtatcctt ttcccctagc atcttttaag agttttggaa aatcatctct taattttaat
     1201 aattcattta aaagtctctc tccattttct cttatcctgt atatcagttc cctgttattt
     1261 ctggtaatta tatctataac tctatcagta atcttgcagg ctatattatt accagcgaat
     1321 gtagaaaagt aatttaaatc aaatcctcta ggataaaatt cctctttaac taaacaggca
     1381 ttcacaggat agatacctcc acccaatacc ttgcctataa gcaatatgtc aggggtgata
     1441 ccttcctctt cacaggcaaa taaaaatccg gtacgaccta agcctgtctg aatctcatca
     1501 atgataaaaa ctactctata tttcttacac aactcactta cctgagtgag atacccttta
     1561 ggggggataa caatacctcc ctctccctgt atcggctcca tcataacccc cgcatatctt
     1621 gctccaccat tctttaactc ctcttccagt acatcaatat ctccataggg tacatattta
     1681 aatccatcct cctgcatccc atgatatccg ccttcagcaa caatgatatt tctcttccca
     1741 gaggataatc ttgccatctt tatggcgata ttgaccgctt ctgtaccgga gtttgtaaac
     1801 accacatact taagaccttc aggggctaaa cttatcagtt tttcggcaac actctcctga
     1861 gactctgaga ttgaagcctg gacaatactg gggagctttt ccccctttat ctcttccagt
     1921 acctcccata tttcagggta attaaatcca aaaggaacag ctccaaaaga ggaaataaaa
     1981 tctaaataac gatttccaac attatcatag agataatttc cttcaccata tgtaaaattt
     2041 ttatctaatt ttaatattct aagttctctc ttaatgtatg gattaatgta atctttaaaa
     2101 tcttccaatc tttttcctcc tgtaataact taatcttttc tattttatca gaactgacat
     2161 ctcaatacaa atgttgttat ggtataataa gtggtatgga gacccttgta agcgtaataa
     2221 tccctacgag aaacagaagg acaattatcg gtaagtgtat agaatgtata ttaaatcaga
     2281 cctatcgttc ctatgaggtt atagtgatag acgatgggag tgaagatggt acagaagata
     2341 ctcttgataa attttccagc aatgataact ttaaatacat aaagcttgga gagaggaagg
     2401 gaccagccta ctgcagaaat ctcggagtaa gagagagtaa tgggaagata cttatcttca
     2461 tagatagtga cattttcgtt ttaccagact ttatagaaag tcatattgaa actcatagaa
     2521 atcatcccga gggaatagtc ggtgttggtc ctgtcatcgc tatttccaag atagatgaaa
     2581 aattacctga aaagggctcg gtattagatt tttctaacgc ttactttgcc tcaggtaacg
     2641 cctctttatc aagagacata tatctaaagg ttagaggatt cgacgaggtc ttcaatgtat
     2701 acggctggga agatatagac tttgggctaa aattaaagag tcttggcata aaatctgtta
     2761 aggtccccag ggctatagga taccattatc agcctctccc caaagaggaa gatattgatt
     2821 cgcttataga gaaggagcgg gaaagggcta gaacggctct ctatctatat aaaaaatatc
     2881 cgaccctgga agtaaagctt atgatacagc tcagctcttt ccataggata ctgaatagat
     2941 ttcttactgg atttggatat atagataaaa aaagggtaat cagaattctc aattctaaca
     3001 ggagttataa cataaaaaga cttgttctta cagtatttct aaccagggta taccttgacg
     3061 ccctggagaa agagttaaaa gttctataac taaagggcag gattatctat cctgccccaa
     3121 gcttcatatt actctgtccc ttcttcttca taagacttaa gataatctat actccactgg
     3181 gcgctttcag ccaggtcact gtccggatac tcatcaatca gtttctgata ttcatttata
     3241 gcctcttcat acttctcgag atactcatag gaagaagcta tgtcatattg agcgtcatca
     3301 gcccattcac tatcaggata ttgttccaga aatgccctgt actcttctat agccttttcg
     3361 tattctcctt catagtacaa atcacgaata cgattatact cggcttcgtc aggagccatc
     3421 tctgcagcag cttcggtttc ttcagccacc tcttctgtct cggtagtctc ttcagttcct
     3481 acaaattctt cagtcacttc ctctgcagtt tcagtggtaa cttcttgctc ctccggtcct
     3541 ccgcctgtag atacagctat tattatagct attactacca cggctactac caaatatggt
     3601 agccacttag gaagattacg tttttccatt ctacatacct cctttttcag ttattattaa
     3661 tcctgtttga ttttagcctc tgataattaa tatgaagaaa cagatcaaat agttcgcaga
     3721 tccatatgga cttttcaaat ggaagataaa ttctatataa tagagttaaa ttaaacaagt
     3781 caggaggtta ttatgaataa gaactactct atagaagaga tactggagat agctgtaaag
     3841 atagaggaga atggagtttt gttctacaga actttagctg aacagaccag agataaaagg
     3901 atcgaagaac tcttcagatt ccttgaggga gaagaaaggg aacatatctc ggtattcaaa
     3961 aacatatatg agaatctcac caaaaagacc tttgagaagg ctttttctga agaagaggca
     4021 aatatgtact tgcatgccct gatagagaat aggatcttca aaaaaccaga agaaattata
     4081 tccactgtta gtagaaacga tggactatct gcagtggata tagctattca ggtagagaaa
     4141 gataccattc tatactacta tgagattctg gaaaacctga cagggaaaga gaaagacctg
     4201 gtaaaggaac ttataaacca ggaaaaaact catgtataca ggcttacaac cctcaagaat
     4261 actttaaggg gctagaaatc tagcccctta tgaactctat atctcaggat taggaacttt
     4321 cttccaatct tccaggaatc tcttcaagcc tatatcggta agtggatggt ggaagagttg
     4381 ctcaagtacc ttgaatggag cagtaactat atgcgctcct aatcttgccg cctgtagtat
     4441 atggatagga tgccttacac ttgccactat tacctctgtc ttataaccat agttattgaa
     4501 aatggtaagt atatcttcta ctacctgcat cccctcagtg gaaatgtcgt cccatctacc
     4561 aataaatggg cttacataac ctgctccagc cttggctgca attaatgcct gtgttgggga
     4621 gaaagtaagc gtaacatttg tcttcactcc cctattagat aactctttca ccgccataat
     4681 accctctggg gtcatgggaa ttttaaccac cacattggga gctaaactag ataactttat
     4741 agcctctttg atcataccat ctgcttctgt acttataact tcaaggttca ctggtccgtc
     4801 aacagtattc aatatctcca tcacaacttc tttaaacggt ctattctctc tagaaattat
     4861 tgtcggattg gtggttacac catccagcag cccccacttt tttatctgtc ttatctcttc
     4921 tatattagct gtatcagcaa agaatttcat aatatcaacc tcctaagtaa gcttttttta
     4981 cagcatcatt atttaacagt tctttccctg taccttccat aaccactcta cccgtctcaa
     5041 ttacataagc cctatgagca atctgaagcg ccatataagc attctgctcg acaagaagga
     5101 tagtaactcc ctgtccattt atctccttaa tagtctcgaa gatattaagc actaaaagag
     5161 gggcaagtcc cagagacggt tcgtccagta atattactct cggctttccc ataagggcac
     5221 gggatatagc cagcatctgc tgttctccac cactcaatgt tcctgccttc tgcttctgcc
     5281 tttccttcaa cctcgggaac agggagaata cccagtccag ttctctagat atatcgtctc
     5341 ttctcagata agcccccata agaagatttt ccataactgt aagattacta aatactcttc
     5401 taccctctgg tacctgtaaa atacccaacc ctgctatttc atttggagcc atcctggtta
     5461 aatcctgacc atcaaaagta atggtcccct ttttaggtct gataagtcca gatatcgttc
     5521 taagggtagt tgtcttccct gccccgtttg cacctataag tgtcacaatc tctccgtcat
     5581 ttaccgttaa agatacctct ttcagagctc ttatgacacc gtaatataca tctatccctt
     5641 ctatcttaag catctatctg gtcactcctg taaaatggaa attaccaagt tttaaggctg
     5701 gcactaaagt gggatatata aaatattctc cagctatatg taaatctttt cccacagctt
     5761 cggtagagtt taaagcctcc agtatactct gggtaaacct catattcttc accggtctgg
     5821 tgatctcccc atcctctata agaaacgtac catctcttgt catcccagta attatagttt
     5881 taacagggtc taccatatta gtatagtgga atcttgtaac cagaatacct tttttagtag
     5941 aagctatcat atcctctaag gtattatccc cgggtaagag cgtaaggtta aatggaagcg
     6001 gaccgtctgt acttggttgt ggaagggcat tcccggttga agttttccct tctttcttag
     6061 catatttaaa atcatagaca agattcctgg caatccctcc ttcaataagg ataacatcct
     6121 gtctgggtag accttcaaag tcgaaaggaa ggctgatagt cttagaatgg gatggataat
     6181 cccatatatt tatcagttct gatgctatct tttcactcag ttttccaacg agaaaactcc
     6241 tcccttcttc aaattgcttg gcaccgaaac ccataaggcc taaaaaagaa accagtgtac
     6301 ctgcagcctg tggttccagt atcaccgtat aatatccagg agaaagttct acaggatttt
     6361 gcccatttct tgccttattg cttgctatct tagcaagaga tataaaatct atactatcca
     6421 ggtccttttc catatcgtat acataaccac ttgaatctcc atcctgaacc acagcttcca
     6481 aagaacacat cgaaacacta tcttcaacac ttattccgtt tgaatttact atggcaacag
     6541 tggtatctgt taattctaac gcacctgagg cgttcaatcc atcggtagcc tctataaaat
     6601 caataacttt ttgggctaac ttttcaggat ctgtttcttt ctgtacagta tctttcttag
     6661 acctgatagg gaggggtctg ggaagtccgt aataatcagg atcaggtgga gtcagtttag
     6721 ctatacttat agccctctcc agtgcttcat ccagagcgtc ggtcgaaagc tgatttatcg
     6781 ataccttccc ggttctatta tcaacaatca cccttatgga caggtcaata tcatctactg
     6841 agacattctg atgtatataa gaattagcaa atcttgtaag tgtccgattg gtatgtacaa
     6901 gtaggacctc agtatctatc ctcctagctt tttcaaggac atgcattaat atctcaccgt
     6961 acatctacct cacccccacc ctgacatttc taaatcttgc tggagcagtc ccatgtccaa
     7021 catgagctat ctgtcctggc tgtcccttac cacagtttgg aacaccccat atcttccact
     7081 caccaggacc acataccgca tccaaagaac tccagaactc tggagtaata ccggtataat
     7141 tcggattctt taatatcccc atcaactttc cattcttaat ctcccatgca atctctaatc
     7201 caaactgaaa atttaacctc ctatcatcta tagaccagct cttgtttgta ctcataaata
     7261 tcccatcttc cgtgtccctt ataagatctt ccagtgtacc tcctccaggt tccagattta
     7321 tattggtcat cctgataaga gggatattcc cccaaccatt agccctgcag gttccattgg
     7381 aggtttcacc caaaatataa gcggtctccc tattggtcag aaaattctga agcacacctt
     7441 ctctaataac ctctatcctc tgtgccggga ctccttcatc atcatatcca aaagaaccca
     7501 atccacctgg cagggttgca tctgctgaga cattcataat cttagatcca tatttcagtt
     7561 tacccctatc tccaattgag ataaagctgg tgccggcata acttgcttcc attccaagga
     7621 ttctatccaa ctctatcggg tgaccaatgg actcatgtac ctgtaaagca acctgagacc
     7681 catccagtat taatgttgtg gtaatggatg gacactgggg tgcatccagc agggctgagg
     7741 cctcctcggc aaccctctgg gcattattta acaggtccat ctcttcgata agctcatatc
     7801 ctctggtttc atactgcccg ccaaaagagt taggataact tctgcgctgg agctcacctt
     7861 ctccaatagc atacgcagag atacctccac cactctctat tatatgctga agaatttctg
     7921 tcccttcact gtttacaaat agcttctcag tctcccaaaa atccatacta ccctcagcta
     7981 tctttacatc tcttatctta cccataattt ttgccgattc aataagtaga cttacctttt
     8041 tatccaagga gacatcaaac ggatttacct taacattatt actccatgtt gctattatct
     8101 taggagctgg agataaaata acatcttcct ttttagccag actacttgct ttggcaacct
     8161 gaatagctct tttagcagat tccaatatct tatctctctg taggtcagat gttccagcaa
     8221 aaccccaggc tccattaaca ataactctta tacctatccc ctcttctgta tggtcaatca
     8281 gagattccac cgactcattc tttacattta taccctgata cctcctcctt agtaccctta
     8341 cctcgcaaaa agaagctcca ctccccatag ctatatctaa tgcctcatgg gcaatatctt
     8401 tcatatcatc ttctcctacc tataatgttt taataaacca gtctgagaca agacctaata
     8461 cttctttttc ccagggaact gagttaaagg tatggtctgc accatctata actttcagtc
     8521 tggtctccac tgccctgcct ttaagtgctt cataatattt atacgcagca gctacaggaa
     8581 ccacattatc acctgatccg tgtattataa ataccggtcc tttaaaattt ttaatcatct
     8641 ccaccggctg tatatcgggg agttccccta taaatcccag tcctaattgc catccaccat
     8701 aatctacaaa accttccgcc cttaatttat cttccggata ctgagttcta aaaccctcag
     8761 caagagaaga taattctgca actgcagaca aaagtacagt agattttata tcttctcttt
     8821 taccagagac ataactggca atacctcccc ccatacttaa acccaatacc cctatcctgg
     8881 aaggatccac acattcttgc tctctcaaaa aatccactgc gacaagggca tcttcaactt
     8941 ctccggagaa tgtcatctca ataaattctc cctcactatc acctgagcca taaaagtcaa
     9001 atctcaatac atttattcca atagaggcta atttcctcgc aatcttaaca aataagcgat
     9061 gaggctcaat cttattcccc gtaaaaccat gaaagagtac aacacctggt tttctttccc
     9121 catcatctac actatgaagg ataccaacta acctgtgccc ttcttttcca aaaacaacag
     9181 ggatttccat acagtatcat ctcccttttg attaatttta ctataggata acacaaaaaa
     9241 agaaaagaat gcaaatagaa aaagaaatta ctgataaaaa cagggagctg agttttataa
     9301 accagccccc gcagaattaa tccaacttat gaaaaaatat tttttatatc ttcctctggc
     9361 tctgtgatca atctgagatc atactctgat ctgagaacat ttagtatatc atcattgacc
     9421 caggcaggaa gtatgggtcc tatgtatata ctcttaatac caagggcaag caggctccac
     9481 agaatagcca ccgctttttg ctccatccag ctgagaacca gggtaagggg gagatcattt
     9541 actccaaccc caaaaagctc actcaatgcc tgggctatct ctatggctac tatagcatcg
     9601 ttacactgac ctatatctat caatcgtgga actccatcta tttcccccag gtccaggtcg
     9661 tttatccgga actttccaca ggcaagggtg agaataatcg tctctttagg taacttttca
     9721 acaaactctc tataatactg gctcttctta agaggtgcat cacaaccccc taccaggaag
     9781 aatcttctaa tctttcctgc ctctaccaga gacttaatct tttcagcaag agaaaggatt
     9841 accgatttag aaaaaccggt aactaaaaat tcttctttat gctcatcttc aagatcaggg
     9901 agacttttcg ccttttctat caccggagta aaatcatacc catcgatatg tttaactcct
     9961 ggcaagccag taactccggt ggtgaacatc ctgtccttat aaccctctaa aggtggcaaa
    10021 acacagtttg aagttccaag gatagctgct ggatatttgt taaacagttg tctctgatca
    10081 tgccatgctt taccaagatt tcctaccaga tggctatact tctttaatcc aggatatcca
    10141 tgtgctggta aaagctcaga atgggtatag acatttattc cagtcccctc agtttgtttc
    10201 agaagctctt ccagagcttt tagaccatga ccggtagcaa ttatcccctt accctttaca
    10261 gaaccggttc taaccctggt tgggactggc tctccatact tttctatatg ggcagttttc
    10321 aacaatctca tagtctttat attcatcctg ccagtctcaa gtgccagagc tacgaatttc
    10381 tccggatcaa agttgacatt ggtaagggtg gagtaaaaag cagattccag aaaagcatcc
    10441 acttccggat cggtatagcc cagttctctg gcatggtata ggtaggcggt tatacccttt
    10501 attgaaaaga gaagattatc ctggagtctt gccaccgtag gcacctttcc acatacccct
    10561 ttaacagtac aaccagtacc attcgtagtc catgaacact ggtagcagaa catatcaagt
    10621 ttctctgcac tgttcatctt caaaacctcc tgtactattt tgatattatg ttaacatagt
    10681 tgattaattt actcaatatt ataaaataca gcaagctaaa aagcaagaaa atatgaattt
    10741 ttacaattag aaaagacatc gtgaattcaa aacaactact attcgaaggg attaaaaaaa
    10801 tggctccccg ggtaggactc gaacctacaa ccaaccggtt aacagccggt cgctctgcct
    10861 gttgagctac cgaggaacac gataatatta taagacattt tacaaattag tcaagagatc
    10921 tttatatcca acctgtgagt ttagctaata gtctggtgta atctaggaaa ttccgccagg
    10981 atatgtcggg gggaacccca tggtcgcagc tgggtatata accaccatct tttaacagtg
    11041 gcggtactac cctcaaaact tctctttcca tctctttccc acccttggcg atagccctct
    11101 tatcgatacc tcctatgtaa gccatacttt ttccaaatct tctcctgaat tctactatat
    11161 cattaccagc agctacctct acaggagtgc agcaattaaa gcctgcctct atccagaggg
    11221 gaataagttc tcctatatag ccatcggagt ccatagaaat aattgggcag ccactttttc
    11281 taagttctgg gatccatctg atatatgctg gaaagaggaa ttctcttacc attcctggcg
    11341 atatcatact gtgctcttta taagccatat cttcagagat caggacatag tccaattgca
    11401 ctctggatat tattcttctt aagacttcca gtacaaagcc agcccagaaa tcaatcatct
    11461 ctttgacaaa atctggtctc tctatcataa aaatacacag attctccagt ccacaccatt
    11521 ccctcaactg ccagaatgga ccatcaaaac ttaaacttaa tacataactt ctatcttgga
    11581 cccttttaca cctgtcctca aaatcttcag ggaatcttcc aggagaattc ggatcatatc
    11641 ttttcttcat ctcttcccaa tctgagtaat cttctaccgg aaatttatac cattttctgg
    11701 ttacaaaatc ttttgcctct ctgaggtatg tataatcata ctggtcagat atttcaacta
    11761 tagcccccat ccaatcctgc actatataat gaccatctct atgttctatt atcttctcct
    11821 caaactgggg gatcatctta aaagataccc cgagatcaat tctttcttcc tctttctcct
    11881 tctctatacc cagaatttct agtagtgcag aataatagtt ctttccctcc ggaagtccct
    11941 cttccctcca ccttttcaga gttgattccc ttggacctcc aggcatcaat ggaattttat
    12001 caggctttcc aaatgtaagt gtttctacaa atctttccct agaagtcatt atattacctc
    12061 cagtaaatct ctaaccctga tcgggtttcc agtagctata gatttattgg ctgaaattcc
    12121 aatcattatt gacatagcac cagctctgga atctgccata tgtccaagag ggtctggctg
    12181 attttttgta aagagcatat tttgaagtct ttcatcagag ccaccatgtc caccggtcac
    12241 tttaggaatc tcatatgtta ctatctcatc ttttctgttg aatagtttta tatactggtc
    12301 aggttctctt acataatgac cactgtgaaa ctcttccgcc tctatccttc catcagttcc
    12361 agtgatggac attctccatc cttcataggg actgtaggct actagggaat aagttaagag
    12421 agctcctttc gaatatctta cagttacact catcgtatcc tctatatcta tctctggaga
    12481 aaatacgcat ccgtcacggt agtatccatc tatatcttct gcatcaaagt agaatacctt
    12541 cataaaaggg tctttagagt aatccacaaa aaattcacag ctatctttat actgacaggt
    12601 tagacatctt gctcccctat tctctcttgt tggtccgtag aatcttctcg ttccatttgc
    12661 atatacaact tccgggtcct cctctatcca ccagtttatt atatcaaaat gatgggtagc
    12721 cttatgaacc agaagacctc ccgagttctc cttcctcctg tgccatcttc taaaataatc
    12781 tgccccatgt ctggtatcca gaaaccattc gaaatctaca ttcagtatct ctccaacaac
    12841 tttttccttt aagagttcct ttatccttgt aacatatggg ttgaacctgt agttaaaggt
    12901 tactattatc ctcttccctg tctttttttc tgtctccaac atagccctgc atttttcatc
    12961 gtctatggtc ataggctttt ctactataac gtcacatcct gctttcatag cccttattgc
    13021 atattcatga tgatatctat ctatagttgt aactatcacg cagtcaggat gtgtttcttc
    13081 taacatctta tcaaagtcgt caaagactgg gatccctccg cagtaattac ttacatattc
    13141 tgccctcttc ctatttatat cgaatatacc tactaatttt gcatagtcct tgaatctctc
    13201 agttattggg atggcataca tatagaatcc tctgctactt gcaccaacca tcacatagcg
    13261 tttcatcttt tctccctcct agtacctgta ggcttttact cctccaagtt cctctatatt
    13321 agctatctct cccctgctat ctcttgggtt taccagaaca acatttctat cgcttataca
    13381 ggatataaga tctttaatat cgaaatatct tgctattcca tatatctcaa gtctagggtc
    13441 atacctgtag tatctggaag agattaactc ctcataagaa tagagaatat cttccaggta
    13501 tatctctttt acactttcat caatcacact ggcaaatagc gcatatatgg agcttactcc
    13561 tttcccagct atagaaagag tagcttttgg attaacctct ttaacgtatt cgaaagctct
    13621 caagatatca aaaactctca aggcagtcat agatgtccct agcattatta gatcaaaatt
    13681 caacttatac tcagttccgt aatactcata aaatccatga agatttatat ctctacactt
    13741 tactgaacca attcctctag ggtcatatac aaatacatct cctctagata ggaaactcct
    13801 tatcaaatca aattctttca atacatcttc tgttccatta tctaaaagta acaatgtgca
    13861 tagattacta ccttctccag agaaatatac tccagctgtt gttatgtctg tttctgtaaa
    13921 cataaatacc ttcttatagg ggaacccatt aaaagaccct gattctatcg tcctcggata
    13981 gagaggtctt ttatc
//
LOCUS       DAOTKU010000003        10898 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_6366_length_10898_cov_3.425528, whole genome shotgun sequence.
ACCESSION   DAOTKU010000003 DAOTKU010000000
VERSION     DAOTKU010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10898
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6366_length_10898_cov_3.425528"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="P5310_00135"
     CDS             <1..616
                     /locus_tag="P5310_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HRU31807.1"
                     /translation="GALERETNSIILAADETEILGIVGVSDAPKDNAREVIEELKSLH
                     IKTIMLTGDSKKNALYMKEKLGLDDVIADVLPQEKMMIIKQLQSQGRVIAMVGDGIND
                     TPALKQADVSIAMGTGTDIAIEAGDIVLVNGDISNVLNAIKLSKEIFKKIRQNLFWAF
                     FYNIIAIPLAGLGLLHPVIAEIAMAFSSINVITNSLRLRRFNFD"
     gene            609..866
                     /locus_tag="P5310_00140"
     CDS             609..866
                     /locus_tag="P5310_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensing transcriptional repressor"
                     /protein_id="HRU31808.1"
                     /translation="MTKNLEALNLLKTARGQIDGVIKMIEDERYCIDISKQLLSLISL
                     LRKSNGLILKNHMETCVREAIATEDIENKMKELEEILSYLV"
     gene            991..2664
                     /locus_tag="P5310_00145"
     CDS             991..2664
                     /locus_tag="P5310_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009611345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HRU31809.1"
                     /translation="MDIKIVIGGEAGQGIQTISNIMSKFFLRSGYYVFTEQSYQSRIR
                     GGHNFTQVRVSSEPVSSPDDGIDILVALNRETVDIHSSETGGIIIYDNELIKDLSGDK
                     LFGVPLQKIAIEKTQNKLAVNSVACGVLAGLLDGDISILERLMREQFKDPDIGEKNVL
                     AVRSGYEIGKSNRTLPFKITPLSQKGRLLIDGGSAVGFGAIVAGCRFLSAYPMTPGTA
                     VMNFLAGHSKRFNIVVEQAEDEIAAINMVLGASFAGVRSMVTTSGGGFALMVEGLSLA
                     GMTETPCVIHIGQRPGPATGLPTRTEQGELLFVINAGHGEFPRYITAPRDAEDAFYKT
                     IKAFNLADKYQIPSIILTDQFLIDSMTTIDSLDVKDIKIERHIVTEPDNNYARHLLTS
                     SGISPRALPGTEGILVITDSDEHDEEGHITEDLEIRKNMVEKRLKRGEYLKEDVEEPV
                     FFGDKDSSIVLTGWGSTYGILKEAVNRLTKEGISVSLLHFSDVYPLPDKTLNSLRDKR
                     FISVENNATGQLAQLIRRETGINITEKILKYNGRPFTPSEIVRRFKEIC"
     gene            2658..3512
                     /locus_tag="P5310_00150"
     CDS             2658..3512
                     /locus_tag="P5310_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HRU31810.1"
                     /translation="MLDIEVYNNNQIPAWCPGCGNFSILRCMKQALSELDISPDRLLM
                     VSGIGQAAKAPHYFRCNLFNGLHGRDISVGAGAKIANHELLVISESGDGNTYGEGGNH
                     FIHNIRRNINMKVFIHNNQIYGLTKGQASPTSDTGMKTKVQVDGVINPPFNPIAVAIA
                     LNASFVARSFSGDPEHLVSMMKSAITHKGFAILDILQPCVTFNRLNTFQWYRQRVYKL
                     DDSYDPTDRKLAFEKALEWGDKIPIGVIYKNNRPVYEEQLQQLKDGPLYKQPVTGIDY
                     SDVLKEFI"
     gene            3565..4416
                     /locus_tag="P5310_00155"
     CDS             3565..4416
                     /locus_tag="P5310_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU31811.1"
                     /translation="MKIKLTAAFLIILSLITQCAFAQNSWEFGASKSLVESRYFLDAR
                     VDYETMHLYLGNLQGEGDILVIPFETRGVLFSVYDDNMNSFSTILGATPVKQVISAEV
                     KLALEGYNDTAGIYKIGDSWGIGYKRDGTEILGPILGNYEYKNYLTLNSAGKIGFGTY
                     SSLGFASGSQAINLDIGYIYSSSDIFVFPPNSLPFGLGYRERLSSGVILGGKVIPYVC
                     FNDGSWGVNIDTNLRLDNFSINIGLDYYKDELSYAGSLIIGLSDTKHIALRLNPGSAG
                     IAYGESF"
     gene            4428..5192
                     /locus_tag="P5310_00160"
     CDS             4428..5192
                     /locus_tag="P5310_00160"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HRU31812.1"
                     /translation="MHKKKILVALFFILLLVINETLAFAITPKDLLNRVNERAKDVKD
                     SVVDIRMSMSLTGMSSGSGQSGTTSLVYRMKIESIINPPVVRMTYIEPDTFKGTVMLV
                     DTEKKLMSVYSPVTNQVVQSKMEDTQTSTLNINTADPTSMLQDLEKTYDLAVEEKKID
                     KKDAYVLTATLKPNQKGDFSKGIFYFEKDTLNPIKVELFDTKGQPMATIEILSIKYNA
                     GLKAATLRSFPKDAKIVKGGTMQGTPGLPFGTQSNK"
     gene            complement(5520..6896)
                     /locus_tag="P5310_00165"
     CDS             complement(5520..6896)
                     /locus_tag="P5310_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015849001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRU31813.1"
                     /translation="MGRGDVRNIVLVISITGSFLTAFLGSSVNVALPLIGKDLSADTV
                     MLTWINTSYLLATTIFLIPFGRLSDIYGRKKIYLYGILVFTISSLLCGLSNSYIFLIL
                     TRVLQGIGAGMLFATSTAILTSVFPVGERGKALGLSVTAVYIGLSAGPFLGGILTRCL
                     GWRSIFFSSVFIGVITLLLILLRLKEEWIEAKGEKFNLPESILYSATLAGVIYGFTRL
                     PSITGLFLILLGIIGFLIFLVVESRREDPILDIKLFTNNRVFTLSNFSALISYSATSA
                     IAYLLSLYLQYIKGLDPQNTGIILVFQPVMQAIFSPIAGRLSDKFQPGLVASLGMFFT
                     TLGLFLLRFIDANTSILFIIICSVILGFGFGLFSSPNTNAIMSSVDKKFYGIASAMVS
                     TMRMFGQTLSMGVVTIVFTVVIGNVHFQPGYYPLLIRSMKLIFTIFSILCFIGIFTSL
                     GRGRLERD"
     gene            complement(6920..7150)
                     /locus_tag="P5310_00170"
     CDS             complement(6920..7150)
                     /locus_tag="P5310_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012532322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU31814.1"
                     /translation="MEGVMSVDCPNLKTCIFFQDKMKNMPATANLMKNRYCLGDNSQC
                     ARFIVSKALGKENVPSDLFPNQYERAMKLIGK"
     gene            7262..7783
                     /locus_tag="P5310_00175"
     CDS             7262..7783
                     /locus_tag="P5310_00175"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HRU31815.1"
                     /translation="MKRTYRLISIFLLFTIIFYTAQGYGESLKLKKAKVTGVIDGCTI
                     KVTIDGKHDTVRLIGVDSSGIIKINESHGKKALEFTKSKLLKKTVYLEVVSRDRSGRL
                     LAYVWLSPPSWKDDNGRDKEIRSKMFNAILILNGFAKPLFSINLKYQVHFKGYQEEAK
                     GKKLGVWKENKKK"
     gene            complement(7820..8209)
                     /gene="mscL"
                     /locus_tag="P5310_00180"
     CDS             complement(7820..8209)
                     /gene="mscL"
                     /locus_tag="P5310_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019907732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HRU31816.1"
                     /translation="MFKGFRDFIMRGNVVDLAVGIIIGGAFSQIVNSLVSDILTPFIG
                     LLVGVPDFSGFKIGPLGVGKFINTVVSFLLIAAAIYFLIIVPMNKLHEFLDRQKEQTP
                     PQPSAPPEPPEDIKLLREILETLKNKQ"
     gene            complement(8278..9705)
                     /gene="argH"
                     /locus_tag="P5310_00185"
     CDS             complement(8278..9705)
                     /gene="argH"
                     /locus_tag="P5310_00185"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HRU31817.1"
                     /translation="MAQFLWSGKEELPDIVKEFTYSIEDDKNLFYYDILGGMAHTLTL
                     YRGGIIDYDEAKSIIDGLYKLKDKNDINFSLYEDIHTAVEIELTALIGEPAKKLHTAR
                     SRNDQVSLDERLYLRDSLKEVLNLIRDLIKAFLDLAGVSLDIIVPGFTHLQPAQPVLI
                     SHHLLAYVEMLKRDFSRFYSLFPRLNECPLGSGALAGLDFPYDRFLVSEILRFDAPTN
                     NSMDTVTDRDYLIEYIFNALLTSTHISRFGEEIVIWSNPLFGFVKISSGYTTGSSMMP
                     QKRNPDVAELLRGSSGEFLGYLDLLLVTLKGLPLTYNRDLQMDKKVIFRASRSLHIIL
                     EVTRGLLENMELDREKICKNLNDEFLLATDLADFLVNRGIPFRDSHHIVRDVVFYCQE
                     NKKTFSQLSEEEWRKFNEEFLNLPDDFFSFDKAINRRDTYGGTGRSCVEYQLLLNRKW
                     LEETRRLIDALPEVYMEDLVNMIGK"
     gene            complement(9707..>10898)
                     /locus_tag="P5310_00190"
     CDS             complement(9707..>10898)
                     /locus_tag="P5310_00190"
                     /inference="COORDINATES: protein motif:HMM:NF014805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HRU31818.1"
                     /translation="KGHTALILDEALAIADEIGFPLLVRPSYVLGGRAMEILYNKEEF
                     VSFVKYVLGVSPDYPILIDQYVVGKEAEIDAICDGEEVFIPGIMEHIERAGVHSGDST
                     AVYPPFSLSPDVQKTMLDYAYKIAKALNVKGFINIQFAIDKQDKVYIIEANPRASRTV
                     PFMSKATGVQLVHIATRVMLGEKLRDCGLREGILRDPSYYSVKVPVFSFGKLKGVDPI
                     MGPEMKSTGEVMGVDPVFEMALLKGLISAGIKINGKQGILLSLNERFRDEAINSVSRL
                     KSSGFVFYSTGGTYEWLRAEGIESYRVSSLEEVQELFKLHKVNLVVNTPTKGKDSSRF
                     GFKLRRMAVEWQIPTITAIDLLDAISKAIRIYPEGIDYNVYSLDDLHRLLPPLEDRII
                     NPWR"
BASE COUNT         3463 a         2167 c         2251 g         3017 t
ORIGIN      
        1 cggtgccctt gagagagaaa ctaacagtat tatattggct gccgatgaga ccgaaatcct
       61 gggcattgtt ggagtttcag atgcaccaaa agataacgca agagaggtta tcgaagaatt
      121 aaaatctctc catataaaga ccattatgct taccggggat agtaagaaga atgctcttta
      181 tatgaaagaa aagctgggtt tagacgatgt tattgccgat gtcctccctc aggagaagat
      241 gatgataata aaacaattac agtcgcaggg gagggtgata gctatggtgg gagacggaat
      301 aaatgatact cctgccctca agcaggcaga tgtaagtata gccatgggga cagggacaga
      361 tatagctatc gaggcgggag atatagtcct ggttaatgga gatatatcaa atgtcttaaa
      421 cgctataaag ctttctaaag agatattcaa aaagatcagg cagaatctat tctgggcttt
      481 cttctataat attatagcta tcccacttgc agggttaggt ttattacacc cggtaatagc
      541 tgagatagct atggctttca gttctatcaa tgtgataacc aattctctca gattgaggag
      601 gtttaacttt gactaaaaat ctagaggctt tgaacctgct aaaaactgcc agagggcaga
      661 tcgatggcgt aataaagatg atagaagatg agagatactg tatagatatc tctaaacagc
      721 tcctatcgct catatccctt ttgagaaaga gtaacggatt gatcttaaaa aatcatatgg
      781 agacctgtgt tagagaggct atagctacag aggatataga gaataagatg aaagaacttg
      841 aagagatatt gagttattta gtataatgga agagtcccaa ccaacttatt cgggtttagg
      901 gggcaggaaa caaactcctt tacgggcttc tacagtccag gtgatgagtg tggtttcctg
      961 cctctttaat ttagaggcag gaggtaaaaa atggatataa agatagtaat aggtggagag
     1021 gcaggtcagg gaattcagac tatcagtaac attatgtcga agttttttct gcgttcaggt
     1081 tactatgtat ttacagaaca gagttatcag tccagaataa gaggaggtca taattttaca
     1141 caggtaaggg tatcgtctga acctgtatcc agtccagatg atgggataga tatcctcgtc
     1201 gccctgaaca gagagacagt agacattcac agttctgaga ctggtggaat aattatatat
     1261 gataacgaac ttataaaaga cctatccgga gataaattgt ttggtgtccc tctgcagaag
     1321 atagcgatag agaagactca aaacaaactg gcagttaact ccgtagcatg tggagttctt
     1381 gcaggattac tggatggaga catttctatc ttagagagac tgatgagaga gcaatttaaa
     1441 gatccagata tcggggagaa gaatgtcctg gctgtcagga gtggttatga gattggtaaa
     1501 tctaacagga ctctgccctt taaaattaca cctctttccc aaaaaggtag acttttgatc
     1561 gatggtggat ctgcggtagg ttttggcgct atagttgccg gttgcagatt cctctccgca
     1621 tatcctatga cccctgggac ggcggtaatg aactttcttg ccggacattc aaaaagattc
     1681 aatatagttg tagaacaggc agaggatgag atagctgcaa taaatatggt gttaggagcc
     1741 tctttcgctg gagtaaggtc tatggtcacc acctctggcg gaggttttgc ccttatggtg
     1801 gaagggttaa gtcttgcagg tatgacagaa acaccctgtg tgatacatat cggacagaga
     1861 ccaggaccag ctactggatt acctaccagg acagaacagg gagaattgct ctttgttata
     1921 aacgccggac atggtgaatt tccccgatac atcaccgccc ccagagatgc tgaagacgca
     1981 ttttacaaaa ctatcaaggc ttttaatctg gctgataaat atcaaatacc gtcaataata
     2041 cttactgacc agtttctgat agattctatg actacgatag atagtcttga cgttaaagat
     2101 ataaagatag aaagacatat agttacagag ccagataata attatgcaag acacctgctc
     2161 acctcttcag ggatatctcc aagggctctg ccagggacag aaggtattct tgttataacg
     2221 gacagtgatg agcatgacga agaggggcat ataacagaag acctggagat aagaaaaaat
     2281 atggtggaga aaagattaaa aaggggagaa tatctcaaag aggatgttga agaacctgtc
     2341 tttttcggag ataaagattc ttctattgtc cttacaggct ggggctcaac ttatggtata
     2401 cttaaagagg cggtaaatag attaactaaa gaagggatca gtgtatccct attacacttt
     2461 tcagatgtgt atccgctccc agataaaacc ctgaatagcc tcagagataa aagatttatc
     2521 tcggtggaga acaatgctac cggtcaactt gcccagctta taaggagaga gacgggcata
     2581 aatatcacag agaagatttt aaagtataac ggtagaccat ttaccccatc agagatagta
     2641 aggaggttca aagagatatg ttagatatag aagtttataa taataatcag attcctgcct
     2701 ggtgtccagg atgcggaaat ttttccatac tgagatgtat gaaacaggct ttatcagagc
     2761 tggatatttc acctgacagg cttcttatgg tatcggggat agggcaggca gccaaggctc
     2821 ctcattattt tagatgtaat cttttcaatg ggttgcatgg cagggatatc tcggttggtg
     2881 ctggagcaaa gattgccaat catgaacttc tggttatttc agaatcagga gatggcaata
     2941 cttatggtga gggcggaaat cattttatac ataacataag acgtaatatc aatatgaagg
     3001 tctttataca caacaatcag atctatggac taactaaagg acaggcttct cccacatctg
     3061 ataccggtat gaaaacaaaa gtacaggtcg atggtgtgat aaatccacca ttcaatccaa
     3121 tagcggtagc tatagcccta aatgcgagct ttgttgccag aagtttttca ggtgaccccg
     3181 agcaccttgt aagtatgatg aagtctgcaa taactcataa aggatttgcc atacttgaca
     3241 tactgcaacc ctgcgtaact tttaacaggt taaatacatt tcagtggtac aggcagagag
     3301 tttataaact ggatgatagt tatgacccga ccgacaggaa acttgccttt gaaaaagctc
     3361 tggagtgggg agataagatt cccatagggg ttatatataa aaataatcgt cctgtatatg
     3421 aggaacagtt gcagcagtta aaagacggtc ctctctacaa acaacctgtc acagggatag
     3481 attattctga tgtactcaag gagttcatat aattacgaac atttatcata aataaccagt
     3541 attagactat aaggaggatg aaagatgaag ataaaattga cagcagcttt tctgattata
     3601 ctgagtttaa tcacacaatg tgcctttgcc cagaacagct gggaatttgg tgcttccaaa
     3661 tccctcgttg aaagcagata ttttttagat gccagagttg actatgagac catgcatctt
     3721 tacctgggga acctccaggg agagggtgat atccttgtaa taccatttga aacaagaggg
     3781 gtattattct ctgtatacga tgataatatg aattcgttct cgactatcct gggggcaaca
     3841 cctgttaaac aggttataag tgccgaggta aagttagcac tagagggcta caatgatact
     3901 gcagggatat acaagatagg cgattcatgg ggtatagggt ataagagaga tgggacagaa
     3961 attcttggac cgatacttgg aaactatgag tataaaaact atctaaccct gaactctgct
     4021 gggaaaattg gtttcggcac atactcttcc ctgggattcg ctagcggaag ccaggctatt
     4081 aatcttgata ttggatatat ctattcttca agcgacatct ttgtctttcc acccaattct
     4141 ctaccttttg gtctggggta cagggaaagg ctatccagtg gagtcatcct cggaggaaag
     4201 gttatcccgt atgtatgttt caatgatgga agctgggggg taaatattga tacaaattta
     4261 aggctggaca atttttctat taatattgga cttgactact ataaagatga actttcctat
     4321 gctggaagtc ttattattgg tctctctgat acaaaacata tagcactcag gctaaatcca
     4381 ggttcagcag ggatagccta tggtgaatca ttttaaagga ggtaaagatg cataaaaaga
     4441 aaatcttggt agctttgttt tttatattac ttcttgttat aaacgagacc ctggcatttg
     4501 ctattacgcc aaaagacctg ctcaacagag taaatgaaag ggctaaggat gtaaaagatt
     4561 cggttgtaga tataaggatg tctatgagtt tgaccgggat gagttccggt tcaggtcaaa
     4621 gtggtacaac aagtttagtc tacaggatga agatagaatc tataattaat ccccctgttg
     4681 tcaggatgac ctacatagaa ccagacacat ttaaagggac tgtcatgctt gtagacaccg
     4741 agaaaaaact catgagtgtc tattcacctg tgacaaatca ggttgttcag agtaagatgg
     4801 aagatactca gacgagtaca ttaaatataa ataccgctga ccctacctct atgcttcagg
     4861 atctagaaaa gacgtacgat ttagctgtgg aggagaagaa aatagacaag aaagatgcct
     4921 atgtcttaac cgcaacatta aagccaaatc agaagggtga tttcagtaag ggaatcttct
     4981 attttgagaa agacacatta aatcctataa aagtagagct gtttgataca aaaggacagc
     5041 ctatggcaac tatagagata ctgagtataa agtataatgc tggacttaaa gctgccacat
     5101 taaggagttt tcctaaggat gcaaagatag taaagggcgg tacaatgcag ggaactcctg
     5161 gtcttccttt tggaacgcag agtaataaat aggtcgctta gcataaatat aagttggggt
     5221 attaccttca ccctcacccc gccctctccc atcaagggag agggagaaaa ggttgatttt
     5281 ctatgtcaat agtctaagaa aatgtcagtg gaaggaaatg aaacatatga gattaatttg
     5341 tattccgtag gggcaattca tgaattgccc ctacaatttt gtcttttatg ataataatcc
     5401 catgcttttt tctcctccca catctcgggg aattaacctc tatccagccc cccccaatct
     5461 cgggggagga tagaggtggg ggctggaaga gttgaggtat agcccgtata gattgctatt
     5521 taatctcttt ccaatcttcc tctacccaaa gaggtaaaga tacctataaa acataaaatt
     5581 gagaagatgg taaatattaa tttcatactt ctgatcagta aaggataata tccgggctgg
     5641 aaatggacat tacctataac cacagtaaat acaatcgtca ctacccccat actgagtgtc
     5701 tgtccaaaca tcctcatagt actgaccata gcggatgcta tcccatagaa cttcttatcc
     5761 acagagctca taatggcgtt agtatttgga gatgagaata acccgaagcc aaagccaaga
     5821 ataacagaac atataattat aaataatatt gaagtattag catctatgaa tcttaagagg
     5881 aatagcccca gggtggtgaa gaacattcca agagatgcaa caagacctgg ctgaaattta
     5941 tctgaaagtc tccccgctat cggagagaag atagcctgca ttacaggctg aaacaccaga
     6001 attatcccag tattctgcgg gtcaagacct tttatatact gcagatagag acttaaaagg
     6061 taagctatgg cagaagtggc gctgtaactt atcagtgcag aaaaattgga gagggtaaat
     6121 accctgttat ttgtaaaaag ttttatatcc agaatcggat cttctctcct ggactcaacg
     6181 actaaaaata ttaaaaatcc gataatcccc agcaggatca ggaataaacc tgtaatagaa
     6241 ggcaatctag tgaagccgta tatcacacct gccagggtgg ctgagtagag gatagactcc
     6301 ggaaggttaa acttctcccc tttagcctca atccattctt ctttcaatct taataatatc
     6361 agcagcagag tgataacccc tatgaatacg ctggagaaga atatactcct ccatcccaga
     6421 catctggtca gtatcccccc taaaaatgga cccgcagaaa gtccaatata tactgcagtg
     6481 acactcaaac ctaatgcctt tcccctctct cccacaggaa agaccgaggt caatatcgct
     6541 gtgcttgtag caaagagcat accggctcct attccctgca ggacccttgt caatattaga
     6601 aatatataag aatttgataa accacataga agcgatgaga tggtaaaaac cagaattcca
     6661 tacagataga tttttttcct cccatatata tctgatagcc tgccaaaggg gataaggaat
     6721 atggttgtgg caagaagata tgaagtattt atccaggtta gcattacagt atccgcggat
     6781 aaatcctttc ctattaaggg taatgctaca ttcacagaag aaccaagaaa tgcggttaaa
     6841 aaagaacctg tgatagagat aactagaact atattcctga catctcctct acccaagtct
     6901 acctccgtat acctgcaaat tatttaccta tcagcttcat tgccctttca tattgatttg
     6961 gaaacaggtc agatgggaca ttttctttac ctaaagcctt tgacactata aatcgagcac
     7021 attgagagtt atctcccaga caataacgat tcttcatcaa atttgccgtt gctggcatat
     7081 tcttcatctt gtcctggaag aaaatacagg ttttaagatt tggacaatcc acactcatca
     7141 ctccttccat aagaatttta aattgaaata attataccat ctttaaattt ctattcaaat
     7201 tatatcagaa agagctataa tgtaagtgat accaatttta aagtttaagg aggcgaaaca
     7261 gatgaaaaga acttacaggc tgatttcaat atttttactt tttaccatca tattctatac
     7321 cgcccaggga tatggggaat ccttaaaact taagaaagcc aaagttactg gagttattga
     7381 tggctgtaca attaaagtaa ctatagatgg taaacacgat acggtgagac ttataggggt
     7441 agatagttca gggatcatca agatcaacga atcacatggg aaaaaggcgc tggagttcac
     7501 caaatctaaa ttactgaaga aaacggtgta tctagaggtg gtctctcggg ataggtcagg
     7561 aagattactg gcttatgtat ggttgagtcc tccatcctgg aaagacgata atggaaggga
     7621 taaagaaatc aggtccaaga tgttcaatgc catcttaata ctaaacggtt ttgccaaacc
     7681 tctattcagc atcaacttaa aatatcaggt acactttaaa ggttatcagg aggaggcgaa
     7741 agggaaaaag ctgggggtgt ggaaggaaaa caaaaagaaa taaagacagc ttgtatatat
     7801 ctctctggca aaactgaaat tattgcttgt tcttcagcgt ctccagaatt tctctcaaga
     7861 gtttaatatc ttcaggtggt tctggaggtg ccgatggctg aggaggagtc tgctcctttt
     7921 gtctgtctaa aaattcgtga agtttattca taggaacgat aattaaaaaa tagatagcag
     7981 cagcgataag aaggaaactc acaactgtat ttataaactt tcccaccccc agtggtccta
     8041 ttttaaatcc agagaaatcc ggaacaccta ccagcagacc aataaatggt gtcaatatat
     8101 cagacaccag agagtttaca atctggctaa aggcaccacc aataattata cccacagcaa
     8161 gatccaccac attaccacgc attataaaat ctctaaatcc cttaaacatt ttttccctcc
     8221 cttttttctt cctctcccat caagagagcc ggaagataaa aatcgtagct aaaatctcta
     8281 ctttccaatc atattaacca gatcctccat atacacttca ggcagagcat ctattaatct
     8341 cctggtttct tccagccact ttctattgag gagaagctga tattctacgc aggacctacc
     8401 tgttccacca taggtatctc ttctatttat agctttatcg aaagagaaga aatcatcagg
     8461 tagattcaaa aattcctcgt taaacttcct ccattcttcc tctgagagct gactaaaagt
     8521 ctttttattc tcctggcagt aaaagaccac atctctcact atatgatgag agtcacggaa
     8581 tggaatcccc ctgtttacaa gaaaatctgc caggtctgtc gcaagcaaga actcatcatt
     8641 aaggttttta catatcttct ctctgtctaa ttccatattc tcaagcaatc cccttgttac
     8701 ctccagtata atatggagtg acctggaagc cctgaagata accttcttat ccatctggag
     8761 gtctctatta tatgtaaggg gtaacccctt tagggtaacc agaagaaggt ccagataacc
     8821 gagaaattct cccgatgatc ccctcaataa ctctgctaca tccggattcc tcttctgcgg
     8881 catcatactg ctaccggtag tataaccgga ggatatcttt acaaatccaa agagaggatt
     8941 gctccatata acaatctcct caccaaatct gcttatatgt gtagatgtta aaagtgcatt
     9001 gaagatatac tctataagat agtccctgtc tgtcacagta tccatactgt tattggtggg
     9061 agcatcaaac ctgagaatct ctgatacaag aaatctatcg taggggaaat ccagacctgc
     9121 cagggcaccc gaacctaaag gacactcgtt cagtcttgga aatagagaat aaaaccttga
     9181 aaaatctctc tttaacatct caacatatgc caggagatga tgagagataa ggacaggctg
     9241 ggctggttgc agatgagtaa aaccaggaac aataatatca agagacacac ctgcaaggtc
     9301 caaaaatgcc ttaatcaggt ctcttataag attgagtacc tctttaagag aatccctgag
     9361 atagagtctc tcatcaagag acacctggtc gtttctactt ctggcagtat gtaatttttt
     9421 agctggttct ccaataagtg ccgtgagctc tatctcaacc gcagtatgga tatcctcata
     9481 cagggaaaaa ttgatatcat ttttatcctt aagtttataa agaccatcga ttatactctt
     9541 agcttcatcg taatctatta ttccacccct gtacagagtc agggtatgag ccatccctcc
     9601 caggatatca tagtaaaaaa gattcttatc atcctctatc gaataggtaa attctttaac
     9661 tatatcaggc aattcttctt taccagacca taaaaactgt gccatatcac ctccagggat
     9721 ttataatcct gtcctcaagc ggaggaagta atctgtgcag gtcatccaga gaatagacgt
     9781 tataatctat cccctcagga tatattctta tagccttact tatagcatcc agaaggtcta
     9841 tagctgttat ggttggtatc tgccattcta ccgccatcct tctcagctta aatccaaatc
     9901 tgctggagtc ttttcctttg gttggggtat tgacaaccag atttacctta tggagtttga
     9961 atagctcctg gacctcttcc agagaagaga ccctgtaact ctctatcccc tctgccctga
    10021 gccattcgta cgttccaccg gtagagtaaa atacaaagcc ggatgacttt aatctgctta
    10081 cagagtttat tgcctcatcc ctgaatcttt catttaaaga aaggagaatc ccctgcttcc
    10141 cattaatctt tataccagca gatataagtc cctttaagag agccatctca aaaacagggt
    10201 ctacacccat aacctcaccg gttgatttca tctctggtcc cattattgga tccacaccct
    10261 tcagtttacc aaaggagaaa acaggaactt taaccgaata gtatgagggg tctctcagga
    10321 tgccttccct taatccacaa tccctgagct tctctccgag cataaccctg gttgctatat
    10381 ggacaagctg tacccctgtt gctttactca taaatgggac tgtcctgctg gctctcggat
    10441 ttgcctctat tatgtagacc ttatcctgtt tatctatggc aaactggata tttataaacc
    10501 ccttcacatt aagcgccttg gctattttgt aagcataatc gagcatagtc ttttgcacat
    10561 cgggagagag tgaaaacggt ggatagacag ccgtactatc acctgaatgc acccctgccc
    10621 tctctatatg ctccattatc ccggggataa agacctcttc tccatcgcat atcgcatcta
    10681 tctctgcttc tttgcccaca acatactggt ctataagaat aggataatcg ggagatacac
    10741 ccaggacata ttttacaaag ctcacaaatt cctctttatt atagaggatt tccatagccc
    10801 tgccaccgag gacataagaa ggtctcacca ggaggggaaa tcctatctca tcagcgatag
    10861 ccagggcttc atccaggata agggctgtgt gacccttc
//
LOCUS       DAOTKU010000004        10289 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_6812_length_10289_cov_3.819132, whole genome shotgun sequence.
ACCESSION   DAOTKU010000004 DAOTKU010000000
VERSION     DAOTKU010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10289
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6812_length_10289_cov_3.819132"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1102
                     /locus_tag="P5310_00195"
     CDS             <1..1102
                     /locus_tag="P5310_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRU31819.1"
                     /translation="IGDNDIFYRPGDFYRKNQVKLISGVKATRVDFDGKEVFLETGEG
                     IHYDKLLIATGGKPFVPSIPGPKGNVFTFSNFDDVKAIDRVIFRGASVVVVGAGLIGL
                     KAAEALVERGCNVTVVELAGRILSSILDEEGAGIVQREFEKRGVKFFLENSATKVIGE
                     EDVEAVELKSLDTIPTNVLIFAIGVVPNVDIFRGTSLRINRGIPVNSRMETNIPDVYS
                     AGDVAEALDMLTGENRVVPILPNAYIEGEIAGLNMAGEKVDYDGGFPMNSIGFFDMHI
                     MTAGLLNPSGGMETIKRMEKNKRIYRKLYIDDGKIAGFMFINSIDRTGIITDLMRKRI
                     DISDFKERLLADNFGFLDLPKEFRKEKILERV"
     gene            1170..1859
                     /locus_tag="P5310_00200"
     CDS             1170..1859
                     /locus_tag="P5310_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013429250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU31820.1"
                     /translation="MIRESLTDGYQELRLLNVNGQRYISDGLTFPQSVLEVYGTPGND
                     LGIFMNGLTIKVFGNAQDGVANTMNDGEIVIYGSAGDLAGYGMRGGRIFIRDSVGYRI
                     GIHMKEYQDKIPVIVIGGGSGDFLGEYMAGGRIISLGLNTSEDEITGYFCGTGMHGGA
                     IYIRGKIPEYKLGKETKMIDADENDLEFLKKYIMDFSNYFDLNFDCIMDRPFYKLIPY
                     NRRPYGRLYAY"
     gene            1914..2147
                     /locus_tag="P5310_00205"
     CDS             1914..2147
                     /locus_tag="P5310_00205"
                     /inference="COORDINATES: protein motif:HMM:NF018633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HRU31821.1"
                     /translation="MEDSVKIIDEFGNYSDFQVIDLFNIKDKDYIVLAPQGEIASAFI
                     MRIDGGMLRELTDEEFMEVELYLTSELEDREVN"
     gene            2151..4024
                     /gene="metG"
                     /locus_tag="P5310_00210"
                     /pseudo
     CDS             2151..4024
                     /gene="metG"
                     /locus_tag="P5310_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014043146.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
     gene            4029..4787
                     /locus_tag="P5310_00215"
     CDS             4029..4787
                     /locus_tag="P5310_00215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HRU31822.1"
                     /translation="MLIDTHLHLDEFDDIEDILDRARGVGVSAFVSVGYNIESSRFSL
                     SVARRFQDVFALLGIHPHDAGELTEEFLTWLSEATNDKKVLGIGEIGLDYYRNLSPRG
                     TQIEVFERQLSLAQELSIPVSLHIRDAYREAFSILKDFDVRGVLHCYSGGISFLEEAL
                     SSGFFIGFDGPVTFHNAKETIEVVKRCPLERILIETDAPYLAPEPFRGKINEPANLIY
                     IARKISEIKEIEIEELEKILEENVKKCFPRYALQ"
     gene            4774..5451
                     /locus_tag="P5310_00220"
     CDS             4774..5451
                     /locus_tag="P5310_00220"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HRU31823.1"
                     /translation="MPFSELIFPSRCLVCKRISREIICSDCLSRVSPVPIPVCKICGS
                     PVEKSNICFRCRQNPPYFSRARSYALYDGVIKIAIIRFKFEKKKRIGEFLGNLLGEFA
                     LNLNWKMDVIVPIPLSDRRLKERGFNQSEILALEVGKILKLPVSSGLVRIKETVPSIS
                     LSPAERIKNINKAFLLSDRDLKGKRVLLVDDVYTTGATVNEAGKTLLERGIKEVRVLT
                     LARDLKI"
     gene            complement(5480..5638)
                     /locus_tag="P5310_00225"
     CDS             complement(5480..5638)
                     /locus_tag="P5310_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014063361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HRU31824.1"
                     /translation="MGKWQCTVCGYIYDPKEGDPENGIPPGTPFEGLPDDWVCPDCGA
                     PKEDFEKI"
     gene            complement(5649..6788)
                     /locus_tag="P5310_00230"
     CDS             complement(5649..6788)
                     /locus_tag="P5310_00230"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRU31825.1"
                     /translation="MERVKSAIAGCGGIAGAHVKGLKLLWENDIKTFEIVSVCDIDEA
                     RAEKMAEDIGSFQGYKPAVYTDVDEMLLKEPDIQAVDICTTHNEHHTVAIKCLSAKKH
                     VTIEKPLALTIRYAHMILDSAEKNRCILQVAENYRRALSERTINWVIKSGKIGKPTML
                     FWEDISERLWYWGWRDHKDISGGGWTLDGGVHFADLFRYHIGELEELYAVSKTYNPIR
                     FKDRDKLSDPVVIDVEDTTMSVLKFENGVTGTWVLTNSAVGKKTSFRAIYGDKGSIVW
                     DDGLYTREGHISLEKLEEEYLDSLSSEERERLFPKGIKDTIAIELKEFFDAILGTGEI
                     ETTGEVGLKDEAISMAVYESSWLNMPVKIKDVEECKVENYQKTLM"
     gene            complement(6798..7364)
                     /locus_tag="P5310_00235"
     CDS             complement(6798..7364)
                     /locus_tag="P5310_00235"
                     /inference="COORDINATES: protein motif:HMM:NF014886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase alpha/beta domain-containing
                     protein"
                     /protein_id="HRU31826.1"
                     /translation="MEVTVKYFETKGEVNTEETLNIAKERAKALRINQVVVASTYGGT
                     GKKAVEVFRDTDINLVIVTISSGFTREGWIMPDSVRKELEAKGAKVLTCIHALGDDVS
                     NAFTAKFGGHNPNEIVAEVLRRFSQGMKVAVEIAIMAAEAGLIDVNQEAIAIGGTDRG
                     ADTAIVVKPAYARDFLYFEIKEILAKPR"
     gene            complement(7381..9177)
                     /locus_tag="P5310_00240"
     CDS             complement(7381..9177)
                     /locus_tag="P5310_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRU31827.1"
                     /translation="MPYSLREDEKLEEKVNIDIIKRLLKYLKPYKKEVAIVLLLVGIV
                     MAVELANPYFLKITIDRYIKDKDTRGLISIGVLLLILNLVSALCARARIIRMSSVTNR
                     ILLDIRQDLYNHIQRLSLSFFDERPVGKILARVIGDVNSLHNLFTMSITNLIPDIAMI
                     IATSVIMFSLNAKLALVALSTLPVLVITLFSIQIISRKRWQVVRKKNSTLNAFTHENF
                     SGIRVIKYFTAERYRFNIFNELAWDLRSAFVRAVRINDLFWPMVDFSWGLGTVITFYF
                     GVRMINSGNITVGLLVAFTDYIAMFWRPITDLSNLFNNLVTSMASAERIFEILDINPQ
                     IIERDNAIDVEIKGGVEFRNVSFSYDGEKKVLDNVSFKVNPGEKVALVGHTGAGKTTI
                     INLICRFYDAQEGEVLIDGINIKDIKLECLRRQIGVMLQDTFLFSGSILDNIKYGRLD
                     ANDEEVVQIARWVYAHSFISSLEKGYDTDIRERGLRLSTGQRQLVSLARTLIADPKIL
                     ILDEATASVDTSTERLLQKGISKLLEGRTAFIIAHRLSTIQSSDKILVVENGKIVEMG
                     KHDELMEKKGIYYTMFMSQFRYWEDKEKNLVS"
     gene            complement(9180..>10289)
                     /locus_tag="P5310_00245"
     CDS             complement(9180..>10289)
                     /locus_tag="P5310_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012582943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRU31828.1"
                     /translation="RERHEIEKFFRENHRNYELNVKQAKTLASYGPWMDFLTNISIAI
                     AITIGGLFVIRETMSVGTLVAFSTYVSMLVWPMRMLGWLINALSQCRASLKKIDRFFK
                     EKPEREVIGNPVMLKEIRGNVSFRDVSFKYKDEYVLKNINFELKEGKTLAIMGTTGSG
                     KTTLINLIGRFYEPWEGEILLDGVDIKNISLEVLRSAISYVPQDVFLFSDTVLENIRL
                     GSQVPEEEIIKALETARAYNFVMNLPEGLDTIVGERGLGLSGGQKQRLSIARALVRRA
                     RILILDDVTSSLDMETEHYVQRAIEKHYGDFTKIIIAHRVSAVKNADEIIILENGEIV
                     ERGTHQELLALRGRYFDIYQEQYGKYILDFEEEMV"
BASE COUNT         2932 a         2096 c         2178 g         3083 t
ORIGIN      
        1 gattggagat aatgatatat tttatagacc tggagatttt tacaggaaga atcaagttaa
       61 attgatctct ggagtaaaag ccaccagggt ggattttgat ggtaaggagg tatttttaga
      121 gacaggagaa ggtatccact atgacaaatt actgattgct accggtggca aaccctttgt
      181 tccttctata ccaggtccaa aaggtaatgt ctttactttt tcaaatttcg atgacgtcaa
      241 agccatagat agagtgatat tcagaggagc cagtgtggtt gtggtcggtg caggtttaat
      301 cggattaaaa gcagcagagg ctctggtgga gaggggttgt aacgtcacag tagttgaact
      361 ggcaggtaga atactgagct ccattctgga tgaagagggt gctggaattg tacagagaga
      421 atttgaaaaa agaggggtaa aatttttcct tgaaaactct gcgactaaag ttattgggga
      481 agaagatgta gaagcggtag agctgaaatc tctggataca atcccgacca atgttcttat
      541 ctttgccata ggtgttgtcc cgaatgtgga tatatttaga ggcacttctc tgaggataaa
      601 tagaggtatc cctgtaaaca gcaggatgga aacaaatata ccggatgtat attcagccgg
      661 ggatgtggca gaggcgttag atatgctgac cggagaaaat agagtagtac ccatcctgcc
      721 taacgcctat atagagggag agatagcagg tctgaatatg gcgggagaaa aggttgatta
      781 tgatggtgga ttcccaatga actctatagg attctttgat atgcatatta tgacagcagg
      841 attattaaat ccctccggtg ggatggagac tattaagagg atggagaaaa ataagaggat
      901 atacagaaaa ctctatatcg atgacggaaa gatagctggt tttatgttta taaactctat
      961 agaccgaacc ggtataatta cagaccttat gagaaagaga atagatatct ctgattttaa
     1021 agaaagattg ctggcagaca attttggttt tctggacctt ccaaaagagt tcaggaaaga
     1081 gaaaatattg gagagggtat aaggtggaaa gagacaggac gttaaagaca atagatgctg
     1141 aagggttaca ctataaagtt ttgaatggga tgataagaga atctctgacc gacggatacc
     1201 aggagttaag gcttctcaat gtaaatggtc agagatatat aagtgatggg ttgacttttc
     1261 cccagagtgt cctggaggta tacgggactc ctggaaatga cctcggaata tttatgaatg
     1321 gacttacgat aaaggtcttt gggaatgctc aggatggtgt agccaatacg atgaatgatg
     1381 gggaaattgt catatatggg agtgctggag acctcgccgg ttatggtatg agagggggaa
     1441 ggattttcat cagggattct gtcggttaca ggattggaat acatatgaag gagtatcagg
     1501 ataaaatacc tgtaattgta ataggtggtg gaagtggaga ctttcttgga gaatatatgg
     1561 caggtgggag gataataagc cttgggttaa atacctccga ggacgagata acagggtatt
     1621 tctgcggaac cggtatgcat ggtggagcta tatatatcag gggaaaaata ccagagtata
     1681 aactgggaaa agagacaaaa atgattgatg cggatgagaa cgatctggag tttttaaaaa
     1741 aatatattat ggatttttca aattattttg atcttaactt tgactgtatt atggatagac
     1801 cattttataa acttatccct tacaacagga gaccctatgg tagactatac gcttactaaa
     1861 atgttataat atacaaacag caaaaagttg gctccatcct acaaggaggt tatatggagg
     1921 attctgttaa gataatagac gaatttggca attacagcga ttttcaggtg atagatctct
     1981 ttaatataaa ggataaagac tatatagtac ttgctcctca gggagaaata gcaagtgcct
     2041 tcataatgag gatagatgga gggatgctca gagaactgac cgatgaagag tttatggagg
     2101 tagagttata cctcacatct gaacttgagg atagagaggt aaattgacag atgaaagaag
     2161 ggacttttta cataaccaca cccatatact atgtgaatgc ctccccccat attgggcatt
     2221 cttatactac aattactgcc gatgtgatag ccaggttcaa gaggatgtgg ggttataagg
     2281 ttcttttctc tactggaaca gatgaacatg ggcagaagat actggaggca gcaagggcgc
     2341 agaatagaga ccccaaagaa catgtggata tatattcgca ggtatttaag gatacatgga
     2401 agaagctcca tatttcttat gacgatttta tcaggaccac cgagccaaga catgagaagg
     2461 tggttaaata tatagtcaac aggttatatg agcaggggga tatatataaa ggtatctatg
     2521 aaggatggta ctgtgtcccc tgcgagacat tcttgcagga agatgagctt gttggtggaa
     2581 attgtccaaa ctgtaataga cctgttatat ggaatcagga ggaagattac ttcttcagga
     2641 tgtctgaata tcaggataga ctcttaagac atgtggaaga gaaccctgac tttgtaaagc
     2701 ccgataccag aagacaggaa ctcataagca ggataaaagc aggtataaga gacctgtgta
     2761 tctcaagaaa aggtctcagc tgggggattt ctcttccatt ttctgaagaa tattccatat
     2821 acgtatgggt tgatgcactt ataaactatc tcaccgtggt tggttaccct gaagatgttg
     2881 agagatttga gacatactgg ccagctgttc atcttgttgg taaggatata atgtggtttc
     2941 atgctgccac atggccagcc atgttaatgg ctcttggagt tgagccacca cggcaggtat
     3001 ttgttcacgg attctggaca gtaaagcagg agaagatgtc caagagtaaa gggaatgtga
     3061 tagacccgct ggtaatggca aaaagtctgg ttgataaggg gtttgcagag gatatagctg
     3121 tagatgctgt gagatacttc ctaatgagag aggtcccgct ggggctagat ggagaatact
     3181 ccgatgagaa ctttatacac agggtaaact ctgaccttgc caatgacctg gggaacctgt
     3241 tcttcaggag tgttggtata ataacaagat atggagacgg aattataccc ggcagtggaa
     3301 ctgttgatgg tgagctcctc aatataaaag attccatagt gaaaaattta gatgggctta
     3361 tgacaaactt tgaatataca aaggtattaa ccggtatatg ggaatttata ggtgcggtta
     3421 ataagtatct ggatcagaag gcgccctggt ctctggcaaa agagggaaaa agagaagagt
     3481 ttgacagtgt aatgttcaat gtctttgaat ctataaggaa tatatctata ctgatctctc
     3541 catttatgcc agaggtatcg aggtatcttc tctcttctct tggtttagat gtcgaatctt
     3601 ccaccagaaa agaagcactg gagagctggg ggaaactcaa gcctggctgg aagattccgg
     3661 aagtaagtcc attatttccc aggatagagg tgtagatatg gaagagataa caatagaaga
     3721 atttcagaga ttagacctga gggtcggaaa gatagtggat gcaaagaatg ttgaaggggc
     3781 aaaaaaactg ctccagcttg tagtggatat tggtagcgag tacagacagc ttgtggcagg
     3841 tatagcagag gtttacagcc cccaagacct tataggtaaa gaggttgtta ttgttgccaa
     3901 cctcaagcct gccgttataa gagggatcca atctaatggg atgattctgg cagcctctga
     3961 cggaggtgtt atatccgtta tatctccaga cagagagatg cctccagggg ctaaagtaaa
     4021 ataatctctt gcttatagat acccatctcc atctggatga atttgatgat atagaggata
     4081 ttctagatag agccaggggg gtaggtgtta gtgcttttgt atcggtaggg tataatattg
     4141 aatcgagcag gttttccctc tctgttgcca ggagattcca ggatgtattt gccctccttg
     4201 gaattcatcc tcacgatgcc ggagaactta cagaggagtt tttgacatgg ctttcagaag
     4261 caacaaacga taaaaaggtc ctaggtatag gagagatagg tctggattat tacagaaatc
     4321 tatcccccag aggtacccag atagaagtgt ttgaaaggca gttgtcactg gctcaagaat
     4381 tatccatccc cgtatctctc catataaggg atgcctacag agaagcattc agtattctga
     4441 aggattttga tgtgagggga gttttgcact gttattcagg agggatttct tttctggaag
     4501 aagccctctc ctccggtttt ttcataggtt ttgatggacc tgtaacattt cataatgcaa
     4561 aagagaccat agaagtggta aagagatgtc ctcttgagag gattctcata gagacagatg
     4621 ctccctatct tgcccctgaa ccgttcagag gcaagataaa cgaacctgca aatctgatat
     4681 atatagccag gaagatctct gagataaagg agatagagat agaagagctg gagaaaattt
     4741 tagaagagaa tgtcaaaaag tgttttccca ggtatgccct tcagtgagct catcttccct
     4801 tccagatgtc ttgtatgtaa gaggataagc agagaaatca tctgcagtga ttgtctctcc
     4861 agggtatctc ctgtccccat tcctgtatgc aagatatgtg gcagccctgt ggagaaaagt
     4921 aatatctgtt tcaggtgcag gcaaaatccc ccatattttt ccagagccag gtcttatgcc
     4981 ctgtatgatg gggttataaa aattgccatt ataagattta aatttgaaaa gaaaaaaagg
     5041 ataggcgaat ttctgggaaa tcttcttgga gagttcgctc taaacctgaa ctggaaaatg
     5101 gatgtgattg tccccatccc actttctgat agaaggctga aggagagggg ttttaaccag
     5161 tctgagatct tggctctgga ggttggcaag attctcaaac tgccagtatc ttccggtctt
     5221 gtaaggataa aggaaaccgt cccttctata agtttaagtc cagcagagag gataaagaat
     5281 ataaataaag catttctcct ctcagacaga gatctgaaag ggaaaagagt gctccttgta
     5341 gatgatgtat atacaaccgg tgctactgtg aatgaggctg gaaagacact gctagaaaga
     5401 gggataaaag aggtgagggt tctgacactg gcgagggatt taaaaattta ggggtagact
     5461 tacgtccacc ccatatcttt tagatttttt caaagtcttc ttttggcgcc ccacaatcag
     5521 gacaaaccca gtcatcgggt agcccttcaa atggagttcc aggtggaatt ccattctccg
     5581 ggtctccctc tttcgggtca taaatatacc cgcatacggt gcattgccac tttcccatac
     5641 tttcctcctc acattagagt tttctgataa ttttccacct tgcattcttc tacatctttt
     5701 atcttaacag gcatatttag ccaactcgat tcataaacag ccatacttat agcctcatcc
     5761 ttcaatccaa cctctccggt ggtctctatc tcacccgttc ccaggattgc atcaaagaac
     5821 tcttttaatt ctatggcaat tgtatctttg attccctttg ggaaaagcct ttctctctcc
     5881 tcagaagaga gtgagtctag atattcctct tccagctttt ctaaagagat atgcccctcc
     5941 ctggtataaa gtccatcatc ccatactata gaacctttat ccccatatat agccctgaag
     6001 cttgtctttt tccccaccgc agagtttgta aggacccatg tccctgttac accattttca
     6061 aattttagta cagacattgt tgtatcttca acatctatta ctaccggatc agaaagttta
     6121 tctctgtctt tgaatcttat cggattatat gttttagata cggcgtataa ctcctccaac
     6181 tctcctatat ggtatctgaa caggtctgca aaatggacac caccatcaag agtccatcca
     6241 ccacctgata tatctttatg gtctctccat ccccagtacc acaatctctc agagatatcc
     6301 tcccagaaaa gcatcgtcgg ctttccgatc tttcctgatt ttataaccca gtttatggtt
     6361 ctctcagaga gggctctcct gtaattttca gctacctgaa gtatgcatct attcttttct
     6421 gctgaatcca gtatcatatg ggcgtatctt atagttagtg ccaggggttt ttctatagtg
     6481 acatgcttct ttgccgatag acacttgata gccaccgtat gatgctcatt atgtgtggta
     6541 cagatatcaa cagcctggat gtcaggctct ttaagcagca tctcatcaac atctgtatat
     6601 actgcaggtt tataaccctg aaaagaacct atatcctctg ccattttttc tgctcgagcc
     6661 tcatctatgt cacatacaga tacgatctcg aatgtcttta tatcattttc ccatagaagt
     6721 tttaatccct ttacatgtgc tcctgctata ccaccgcatc cggcgatggc tgactttact
     6781 ctttccatta atccctccta tctcggtttt gccagaatct cttttatctc aaaataaaga
     6841 aaatctcttg catatgctgg ttttaccact atggctgtat ctgcacctct gtctgtccct
     6901 cctatagcta tggcttcctg attcacatct atcagccctg cctctgctgc cataatggct
     6961 atctctaccg cgaccttcat cccctgagaa aatctcctca ggacttcagc cactatctca
     7021 ttaggattgt gccctccaaa ctttgcggta aatgcattac tcacatcgtc acccaatgca
     7081 tggatacagg tcaagacctt tgcccctttt gcctcaagct cttttctcac actatctggc
     7141 attatccatc cttctcttgt aaaccccgaa gatatggtga ctattaccag atttatgtct
     7201 gtatctctga agacctccac agcttttttc cctgttcccc cataagtgga tgcaaccacc
     7261 acctggttta tccttaaagc ctttgccctt tcttttgcta tattcagtgt ttcttctgta
     7321 ttgacttccc ctttagtctc aaaatatttt acagttacct ccatatatca cacctcctga
     7381 tcagcttaca agattcttct ctttatcctc ccagtacctg aactgggaca taaacatagt
     7441 ataatatatt ccctttttct ccatcaattc atcatgtttt cccatctcca caatcttccc
     7501 attctccacc acaagtatct tgtctgagct ctgaatggtg gataatctgt gggcaatgat
     7561 aaatgctgtt ctcccttcaa gcagtttact tatacctttt tgcagcagtc tttcagtaga
     7621 ggtatccacg ctggctgttg cctcatcgag tatcaatatc tttgggtcag ctattaacgt
     7681 ccttgccaga gaaacgagct gtctctgtcc tgttgaaagt ctcaatcctc tttctcttat
     7741 atctgtatcg taccccttct ccagtgatga gatgaaagaa tgggcataga cccatctggc
     7801 aatctggacc acttcctcat cattagcatc cagtcttcca tatttgatat tatccagtat
     7861 acttcctgaa aagagaaagg tatcctgaag cataactcct atctgtcttc tgagacattc
     7921 aagcttaata tcctttatat taattccatc gatcaggacc tctccctcct gtgcatcata
     7981 gaatctgcag attagattga tgatggtagt tttacctgca cctgtatgtc ctaccagtgc
     8041 aaccttttca ccaggattaa ctttaaagct tacattatca agcacctttt tttctccatc
     8101 gtaggagaat gatacatttc tgaattccac ccctcctttt atctctacat ctatagcatt
     8161 atccctttct attatctgag ggtttatatc cagaatctca aatattcttt cagcgcttgc
     8221 catactggta acgaggttat tgaagagatt gctgaggtct gttattggtc tccagaacat
     8281 ggctatataa tctgtaaatg ctaccagcag ccctacagtg atattcccgg aatttatcat
     8341 cctaacacca aagtaaaatg ttataacagt tcccaatccc caggaaaaat ccaccatggg
     8401 ccagaaaagg tcgtttatcc ttaccgccct tacaaatgca gaccttaaat cccaggctaa
     8461 ctcattgaag atgttaaacc tgtatctttc agcggtaaaa tactttataa ccctgatccc
     8521 ggaaaaattc tcatgggtga atgcattgag ggttgaattc tttttcctta ccacctgcca
     8581 tctctttctt gatattatct ggattgagaa tagagtaatt actaaaactg gtaatgtgga
     8641 taaagctacc agtgccagtt ttgcatttaa tgagaacata ataacagatg ttgctataat
     8701 cattgctata tcaggaatca ggttggtaat actcatggta aacaggttat ggagagaatt
     8761 cacgtctcct atcaccctgg caagaatctt ccccacaggt ctctcgtcaa agaaggataa
     8821 cgataatctt tgaatatgat tatacaggtc ctgcctgata tccagaagaa ttctattagt
     8881 tacactggac atccttatta ttctggctct tgcgcagagg gctgagacca gattgaggat
     8941 gaggagcagg acaccaattg agataagacc tctggtatct ttgtctttta tatatctatc
     9001 tatagttatc ttcagaaagt atggattagc aagttctacc gccataacga taccgacgag
     9061 taaaaggact attgccacct cttttttata tggtttcaga tacttcagga gcctttttat
     9121 tatatctata ttgacttttt cttccagttt ctcgtcttct cttaaactgt agggcacgtt
     9181 tataccatct cctcttcaaa atccaggata tacttaccgt actgttcctg gtatatatcg
     9241 aaatatcttc ctctgagggc taaaagttcc tgatgagttc ctctttctac tatctctcca
     9301 ttctcaagta taattatctc atccgcattt ttaactgcgg agaccctgtg agcaatgatg
     9361 atctttgtga agtccccgta gtgcttttca atagccctct ggacataatg ttctgtctcc
     9421 atatcaaggg aagatgttac atcatcgagg attaaaattc tggctcgtct gacaagcgct
     9481 ctggctattg ataatctctg cttttgccct ccagagagcc ccagtcccct ttccccaaca
     9541 atggtatcca gcccttcggg gaggttcatc acaaagttat aagcccttgc agtctccagc
     9601 gcttttataa tctcctcttc ggggacttga gacccgagtc ttatgttttc tagaacagtg
     9661 tcggagaaga ggaagacgtc ctgtggcaca tacgatatag cacttcttaa aacctccaga
     9721 cttatattct taatatccac accatcaaga agtatctctc cttcccatgg ttcgtagaac
     9781 cttccgatga ggtttatcag cgtggttttt cccgaccctg tcgtccccat tatcgccagg
     9841 gtctttccct ctttaagctc aaagttgata tttttcagca catactcatc cttgtatttg
     9901 aaggatacat ctctgaatga tacatttccc cttatctcct ttaacataac tgggtttccg
     9961 ataacttctc tctccggttt ttccttgaaa aatctatcta tcttcttcag tgatgccctg
    10021 cactgggaga gtgcgtttat aagccagccg agcatcctca taggccatac cagcattgat
    10081 acataggtac tgaaagcaac cagtgtccca acagacatcg tttccctgat aacgaagaga
    10141 ccaccaatag ttattgctat cgctatggat atatttgtca ggaaatccat ccagggacca
    10201 tagctcgcca gagtctttgc ctgtttcacg ttcagctcat agtttctgtg attctctctg
    10261 aagaactttt ctatctcatg tctttctct
//
LOCUS       DAOTKU010000005        10240 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_6863_length_10240_cov_3.780265, whole genome shotgun sequence.
ACCESSION   DAOTKU010000005 DAOTKU010000000
VERSION     DAOTKU010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10240
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6863_length_10240_cov_3.780265"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..811)
                     /locus_tag="P5310_00250"
     CDS             complement(<1..811)
                     /locus_tag="P5310_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019908016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRU31829.1"
                     /translation="MIILFDSTYFLTRSQDLSQYLISIYRDIHKFPELSLEEKRTSSK
                     VAEELKKFGLKVRERVGGYGVIGHLKGGKEKPIIALRADMDALPIEEETGLPFSSQNP
                     GVMHACGHDVHTTILLGAARILSEIKDHLPGSAEFIFQPAEETSSGAKAMISEGILDE
                     VDEIYGFHNLPTLETGKIAIKYGAMMGSVDKIEFSVTGRGGHGAIPDMSIDPIVASSA
                     IISGIQSIISREISPFEPAVITIGSINGGSSYNIIPSEVRIEGTVRTLDSEI"
     gene            complement(817..1881)
                     /locus_tag="P5310_00255"
     CDS             complement(817..1881)
                     /locus_tag="P5310_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019908017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRU31830.1"
                     /translation="MNNTIRLNPEVYGLLQEGTVIVAHPLALTEERKLDERRQRALTR
                     YYISAGAGGIAVGVHTTQFEIRKYDMFEPVLRIAAEEVERAGLKRPFIKIAGIVGPVE
                     QAIKEAEIAIKYGYNLGLISMGGLNNLDEDKLIERTEEIARIIPIFGFYLQPSAGGRK
                     LSYKFWRKFVDIPEVMAIKIAPFNRYQTIDVVRSVCESERRNEIALYTGNDDNIIIDL
                     LTKYRFNIRGKIVEKRIVGGLLGHWAVWTKKAVELLEEIKEVRDMEYIPSSLLTRSIE
                     VTDCNSAFFDASNDFYGCIAGINEVLRRQGILEGRWCLNPKEGLSPGQIEEIDRVYRA
                     YPYLNDDDFVKAHLEEWLSD"
     gene            complement(1885..2901)
                     /locus_tag="P5310_00260"
     CDS             complement(1885..2901)
                     /locus_tag="P5310_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019536301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRU31831.1"
                     /translation="MRTIEELETKLAEPSDALISEMAQIKGDIMILGVGGKMGPSLAR
                     LARNAIDKAGVNKKVVGVSRFSDKTLQQELEDYGVQTISADLLDDEQLQSLPEVKNII
                     FMVGYKFGTTGNEHLTWAINTYLPGQIAKRFKNSRIVVFSSGNVYPLTPVSMAGASEE
                     HPTGPIGEYAQSVLGRERVFEYFSLRYNIPVLIMRLNYAIDLRYGVLLEIATLVKEQK
                     PIDLSMGNVNVIWQGDANETALRGIRFSSTPPKILNVTGPETISIRWLAERFGEKFGV
                     RPLFIGKEEDTALLSNASQSHYLFGYPKVSLRQMIEWTAEWIESGRVTLGKPTHFQER
                     EGRF"
     gene            3042..4241
                     /locus_tag="P5310_00265"
     CDS             3042..4241
                     /locus_tag="P5310_00265"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016864662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HRU31832.1"
                     /translation="MAKRVVLAYSGGLDTSVAIRWIQEKYNAEVITLTLDMGQPIDLE
                     AVRKKALSIGAKEAVVVDAKDVFVNHYVKRVIKANALYEGKYPLSTSISRPLIARELV
                     KTAKMYGADAVAHGCTGKGNDQVRIEVSVKALDPELEIIAPIREWGFTREEEIDYAEK
                     YGIPVPVTKKSPYSIDENIWGRSIESGILEDPKVEPPEEPFVWTRSPMDAPNEPEYVE
                     IGFERGIPVSLNGTGLPLIEIITALNSLAGLHGIGRVDMIEDRLVGIKSREVYEVPAA
                     ILIIFAHKELEYMVMERNLLFLKSHLESEYSRLIYDGLWFSHMREALEAFFDATQEVV
                     NGTIRLKLYKGNMIISQRNSSFSLYERALATYEKGDIFDQKKAEGFIYVWGLPLSSSA
                     RKRGKKP"
     gene            4190..5278
                     /gene="argC"
                     /locus_tag="P5310_00270"
     CDS             4190..5278
                     /gene="argC"
                     /locus_tag="P5310_00270"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HRU31833.1"
                     /translation="MGITIELFCQKAREEALINVAIWGATGYTGVELFRILTNHPEVK
                     IRFVFSHTYSGESLSVIYPYLERFEPIALTNEEEFNRIRDKDFDVAFLALPAGVSAER
                     TNLLIHSGKKVIDIGSDFRLKNLDDYKKWYNFEHMYPDLVSEAVYGIPELNRNEIKSA
                     RIVANPGCYPTSFLLGVAPILKNDLLEGNWLYVDSKSGTSGAGKKSSVDMSFSEMFEN
                     IRPYNVGKHRHIPEMEQEAGIMAKRPVKVAFTPQLAPISRGILTTIYAHLKEKVSVED
                     LYSMYGEFYKDSYFVRVLPVGSFPATKGVRGSNFCDIGLAVEDEVAIITTAIDNLVKG
                     AGGQAVQNMNIMFGLKENLGLDIVPLYP"
     gene            5275..6462
                     /gene="argJ"
                     /locus_tag="P5310_00275"
     CDS             5275..6462
                     /gene="argJ"
                     /locus_tag="P5310_00275"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:NF014065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HRU31834.1"
                     /translation="MSITFPRGFIANGIHSGVKKEGLDLGLLLSTCNASASGMFTTNQ
                     VKSPSVIITGERLKSGLLRGIIANSGCANACVGRQGFIDAEYILDRLGGILGEDPKLL
                     GIASTGVIGKRLPMEKIIEKLPILADGLSREGGTLFERAIMTTDRVVKEVKTSFFYNG
                     TDIRIGACAKGSGMIHPNMATLLSFVTTDLNIDHRLLKIALKSSIDASLNAVTIDKDM
                     STNDSVIIMANGRSGNIEIRDEREKGFLLLRDALKDVMVEIAKKIARDGEGASKLIEV
                     EVTGTEDEAQAIRMARAVAGSNLFKSAVWGKEINWGRIISALGANSDPFDPDRISIYF
                     GDVKVVENGIEVEFDKNVVLKILDGKEVLIRISVGDGSGRGKAWGCDLTPEYVYINGS
                     YLS"
     gene            6459..7202
                     /gene="argB"
                     /locus_tag="P5310_00280"
     CDS             6459..7202
                     /gene="argB"
                     /locus_tag="P5310_00280"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HRU31835.1"
                     /translation="MSLVIKLGGSVAENPSALEKVIRGIKDISDGNPFVLVHGGGKQI
                     TSFLSELGIETYFVDGERYTDRRTMEVVEMVLSGLVNKQITSTLCHMGIKSIGLSGRD
                     LNLITVRRKMELGYVGEVKSVDVEPMKRLLDAGFTLVLSPVSETEDGLPCNVNADFVA
                     IEVGKALRASKLFLFTDVTGVLDGNKNIIPLLDVETARDFIERGIIREGMIPKVRSAI
                     SAISAGVKEVWIGNNPVQGTIIRGKDNGP"
     gene            7192..8364
                     /locus_tag="P5310_00285"
     CDS             7192..8364
                     /locus_tag="P5310_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HRU31836.1"
                     /translation="MDLKKEESLYIAQLYKRFPLTIVKGEGVYVYDDEGNRYLDFAGG
                     IAVNSLGYAHPKIIEALTSQARELIHISNLYYTKPQVELAKKLVQLTGLDKVFFCNSG
                     TEAIEASLKFSRRWGKDKGKFKFISAVNSFHGRTMGSLSVTGQRKYQEPFEPLIDGVT
                     FISYNDSDALNEELKKGDYAAVILEPIQGEGGVNPARREFLELARYLCDKYDTLLIFD
                     EVQTGIGRTGAMFAFQNFGVTPDILALAKGLGGGFPIGATIINDKVAQTLHYGDHAST
                     FGGNPLATAVANAVLEEIEKILPRIKEVGGFLEDCLKKKISPLDNVLEIRGIGLIYGI
                     DVKTNARDICERCIKEGLLVITCGNNTVRLVPPLIINEQEIEKGVYILEKVLKEEK"
     gene            8368..9456
                     /gene="carA"
                     /locus_tag="P5310_00290"
     CDS             8368..9456
                     /gene="carA"
                     /locus_tag="P5310_00290"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HRU31837.1"
                     /translation="MGFLLLEDGTLLEGVSFGAEGESIGEVVFNTSMIGYPESITDPS
                     YSGQILVFTYPLIGNYGIQFEDRESDTPLLRGVVVKEYCKTPSHWGIDYDIDSFLKRF
                     GIVGLSDIDTRALTRKLRIYGTLRGIISSEEFLPDRKEELMSRILSWHIEDFDFVRSS
                     CVKEPVYLGNGSDRIVLIDCGVKQGIVRSLLEIGFQVVMVPAWFTREDILNLDPDGVL
                     ISNGPGDPQRLSYLVETTRELIDENVPLFGVCLGHQILGLALGGTTYKLKFGHRGANQ
                     PVKDLITGRVYITSQNHSYVMDSEGLPEDIEISFINLNDKTVEGLKSIRRPIMSVQFH
                     PEASPGPLDTRFLFEEFIKECMKKNYAA"
     gene            9446..>10240
                     /locus_tag="P5310_00295"
     CDS             9446..>10240
                     /locus_tag="P5310_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012342538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HRU31838.1"
                     /translation="MPLRKHIKKVLVIGSGPIVIGQAAEFDYAGTQACKSLREEGISV
                     VLINSNPATIMTDTMMADRVYIEPIEVEAIEKVIAKERPDGLLPTLGGQTGLNMAVKL
                     YEAGILDKYEVELLGTSLDAIKKAEDREFFKNFILSIGESVPESEIVRDLESARSFAE
                     RVGLPLVVRPAYTLGGTGGGFVEKWEDLDEAISVGLNASMVGEVLLERSVRGWKEIEY
                     EVMRDSGGTCITVCSMENFDPMGIHTGDSIVVAPSQTLTNKEYQLLR"
BASE COUNT         2997 a         1888 c         2413 g         2942 t
ORIGIN      
        1 gtatctctga atctagggtg cgaactgtac cttctatcct tacctcagaa ggtataatgt
       61 tatatgaaga accgccattt atacttccaa ttgtaatcac agctggttca aaaggagata
      121 tctctctgct tattatactt tgtatccctg aaattattgc ggaagaagct actatcgggt
      181 cgatactcat atccgggata gccccgtgtc ctcctctgcc agtaacagaa aattcaatct
      241 tatcaacaga gcccatcata gcaccatact ttatggcaat ctttccggtt tctaaggttg
      301 gtagattgtg aaatccatat atctcatcca cctcgtcaag gataccttca gaaatcatag
      361 ccttagcacc tgaacttgtc tcttctgcag gctgaaatat aaactcggca gaaccaggaa
      421 gatggtcttt tatctcagat agaatccttg cagcccccag taggatagtg gtatggacat
      481 catgtccaca tgcatgcata actcccgggt tctgcgagga gaagggaagc ccggtctctt
      541 cctcaattgg gagcgcatcc atatcagccc ttagagctat aatgggtttt tctttacctc
      601 ctttaagatg tccaatcacc ccataacccc ctactctttc tcttactttc agaccaaact
      661 tttttaattc ttcggcaact tttgaagatg tcctcttttc ctcaagagag agttctggaa
      721 atttatggat gtctcgatat atcgagatca gatactgtga taggtcctga cttctggtta
      781 aaaaatatgt tgagtcaaac aggataatca ctcctttcag tcagatagcc actcttctag
      841 atgggcttta acaaaatcgt catcatttaa atacgggtat gccctgtaga ccctatcaat
      901 ctcttcaatt tgccccgggg ataagccttc ttttggattt aaacaccatc taccctctaa
      961 tatcccctgt cttcgcaata cctcatttat cccggctata catccataaa aatcgttgga
     1021 tgcatcgaaa aatgccgagt tacagtctgt tacctctatg ctccttgtaa ggagggatga
     1081 ggggatatac tccatatctc tcacttcttt aatctcttca agcaattcga ccgctttttt
     1141 tgtccagaca gcccaatgtc ccagtaatcc tcctacaatt cttttttcaa ctatcttccc
     1201 ccttatattg aaacgatatt ttgttaacag gtcaattatg atattatcat cattcccggt
     1261 gtataatgct atttcattcc ttctttcaga ttcacaaacc gaccttacca catcgatagt
     1321 ctgatatcgg ttgaaaggtg ctatttttat cgccataacc tcagggatat ccacaaactt
     1381 gcgccagaat ttataggaga gctttctacc accggcagag ggctgtagat agaaaccaaa
     1441 tatagggatt atcctggcaa tctcttctgt cctttctata agtttatctt cgtctaggtt
     1501 attcagtccc cccatgctga tgagtccaag gttataccca tattttatag cgatctctgc
     1561 ctcttttata gcctgttcaa caggtccaac aataccggct atctttataa agggtcgttt
     1621 gagacctgcc ctctcaacct cttctgcagc tatccttaag accggttcga acatgtcata
     1681 tttgcgtatc tcaaattgag tggtatggac gccaactgca atccctccag cccctgcaga
     1741 tatataatag cgggtaagag ctctctgtct tctctcatct aatttacgtt cctctgttaa
     1801 agccagagga tgggcaacaa tgacggttcc ttcctgcaat aacccgtata cctctggatt
     1861 aagtcgaatg gtattattca cccttcagaa cctcccttca cgttcttgga aatgggtggg
     1921 ttttcccagc gttactctcc cactttcaat ccattcagcc gtccattcta tcatctgtct
     1981 gagagatacc ttcggatagc cgaatagata atgcgattga gaagcattac ttagcagtgc
     2041 tgtgtcctcc tctttaccga taaagagagg tcttactcca aatttttcac caaaacgttc
     2101 tgctaaccat ctgatagaaa tcgtctctgg accggttaca ttgagaattt tgggtggggt
     2161 actggagaat ctaatacctc tgagggcagt ctcatttgca tcgccctgcc agattacatt
     2221 cacattcccc atactcagat cgataggttt ttgctccttt acaagagtag caatttcaag
     2281 caagactcca taccttaggt cgatcgcata gttcaatctc attatcagta ccgggatatt
     2341 atatctgagg gagaaatact caaagaccct ctctctacca aggactgact gggcgtattc
     2401 ccctattgga cctgttggat gctcctctga tgcccctgcc atagatactg gtgtcagtgg
     2461 atacacattg ccgctggaaa ataccacaat acgagaattt ttgaatcttt ttgctatctg
     2521 tcctggtaaa taagtgttta tcgcccaggt aagatgttca ttacctgtgg ttccaaactt
     2581 ataaccaacc ataaatataa tatttttaac ttctggtaga ctttgaagct gctcatcatc
     2641 caacagatcg gcagagatgg tctgtactcc gtaatcttcc agttcctgct gaagtgtctt
     2701 atcggaaaaa cgagagacac caacaacttt tttattgacc cctgccttat caattgcatt
     2761 tctagccagt ctggcaagac tgggtcccat tttccctcca acccctaaaa tcattatgtc
     2821 gccttttatt tgagccattt cagagattag agcatctgag ggttctgcca gtttagtttc
     2881 caattcttca attgtacgca tttttaattt tctcctccat cagtctttca tatatctacc
     2941 tcatattcta tcattttatc ggattttatg tttattccgg tattgcataa cttaccctta
     3001 ccttgtataa ttaatcagaa tatatactcg gaggtagaga aatggcgaaa agagtggtat
     3061 tagcatattc aggagggcta gatacttctg ttgccataag gtggattcag gaaaaataca
     3121 acgcagaggt tatcaccctc actctagata tgggtcaacc tatagatctt gaggcagtga
     3181 gaaagaaggc tttaagtatt ggggcaaagg aagctgttgt ggttgatgct aaagatgttt
     3241 ttgtaaatca ctatgtaaaa cgtgttataa aggcaaatgc gctttatgag gggaagtatc
     3301 cactatctac atctatatcc agaccattga tagccagaga actggtaaaa actgcgaaga
     3361 tgtatggtgc cgatgcagtg gctcatggct gcacaggcaa gggaaatgac caggtaagaa
     3421 ttgaggtctc ggtaaaggct ttagaccctg aactggagat aatcgctcca ataagggagt
     3481 ggggatttac aagagaggaa gagatagatt atgccgagaa gtatggaatt ccagtcccgg
     3541 taaccaagaa gtctccttac agcatagatg agaatatctg gggtagaagt atagagtctg
     3601 gaatccttga ggacccgaag gttgaacccc ctgaagaacc atttgtctgg acaaggtctc
     3661 ctatggacgc ccccaatgaa cccgaatatg tagagatagg gtttgaaaga ggcattcctg
     3721 taagtctcaa tggaacgggg ttacctctca tcgagattat caccgccctg aattcccttg
     3781 ccggtctgca tggaattgga agggtggaca tgattgagga taggcttgtc ggtataaagt
     3841 ccagagaggt ttatgaggtt cctgcagcta ttcttattat atttgcccat aaggaactgg
     3901 aatatatggt tatggagagg aacctgctct ttctaaaatc tcatctggag agtgagtatt
     3961 ccagacttat ctatgatgga ttatggtttt cacatatgag agaggcactc gaagcatttt
     4021 ttgatgctac ccaagaggtc gtaaatggaa caattaggtt gaaactctat aagggtaata
     4081 tgattatctc tcagaggaat tcctcattct cactgtatga gagggcactt gctacttatg
     4141 aaaagggaga tatatttgac cagaaaaagg ctgaaggttt catatatgta tggggattac
     4201 cattgagctc ttctgccaga aagcgaggga agaagccttg ataaatgtgg ctatctgggg
     4261 agcaaccgga tatacagggg tagagctttt cagaatctta accaatcatc ccgaggtaaa
     4321 gataagattt gtcttcagcc atacttattc tggagagagt ttaagtgtta tatatcctta
     4381 tctggagagg tttgagccta tagctttgac taatgaggaa gagtttaaca ggatcagaga
     4441 taaggatttt gatgttgcat ttctcgcctt acctgccgga gtcagtgcgg agaggaccaa
     4501 tcttctgatt cactctggta agaaggtcat agatatcggt tctgatttca gattaaagaa
     4561 tctggatgat tataagaagt ggtataattt tgaacacatg tatccggatt tagttagtga
     4621 ggcggtttac ggaatccccg agctcaatag aaatgaaata aagtccgcca gaatagtagc
     4681 caatcctggc tgctacccga caagttttct ccttggagtg gcacccattc ttaaaaatga
     4741 ccttcttgag ggaaactggt tgtatgtcga ctctaaatca ggtacatcgg gagctggtaa
     4801 gaagagctcg gtggatatga gtttttctga gatgtttgag aatataagac cttacaatgt
     4861 tggtaagcac agacatatac ctgagatgga acaggaagcc ggtataatgg ctaaaagacc
     4921 ggttaaggtt gcctttactc ctcagcttgc accaatatcc agaggcatcc ttaccaccat
     4981 atatgctcat ctgaaggaga aggtatctgt ggaagacctc tacagtatgt atggagaatt
     5041 ctataaagat tcctattttg tccgggtatt acctgtgggt tcttttcccg ccaccaaggg
     5101 ggtaagaggc tctaatttct gtgatatagg attagccgta gaagatgagg tggctataat
     5161 tactactgcc atagataacc ttgtgaaagg tgcaggtggt caggctgttc agaatatgaa
     5221 tataatgttt ggattaaaag aaaatcttgg attggatatt gttccattat acccatgagt
     5281 ataacatttc ctagaggatt tatagctaat ggtatccata gtggggtaaa aaaagagggg
     5341 ctggatttag gtctgctttt gagtacttgt aacgccagtg ctagcggaat gtttaccaca
     5401 aatcaggtga aatccccttc ggtaataata accggagaga gattaaagtc tggattactc
     5461 agaggtatta ttgcaaatag tggatgtgca aatgcctgtg taggtagaca gggctttata
     5521 gatgctgaat atatactgga taggcttgga ggcatccttg gggaagatcc gaaacttctt
     5581 ggtatagcgt ctaccggtgt tatagggaaa agactaccga tggaaaagat tattgagaag
     5641 ctaccaattc ttgccgatgg tctgagcaga gaaggtggta cgctttttga gcgggctata
     5701 atgacgacag atagggttgt taaggaggtg aaaacctcct ttttttataa cggaacagat
     5761 ataaggatag gtgcctgtgc caagggttcc ggtatgatac atccaaatat ggctactctg
     5821 ctctcatttg taaccacaga cctcaatata gaccatcgcc tcttaaaaat agcactgaaa
     5881 tccagtatag atgcatccct gaatgcagtg acaatagata aggatatgag tacaaatgat
     5941 agtgttataa ttatggctaa tggaaggtct ggaaatatag agataagaga tgagagagaa
     6001 aaaggattcc tcctcttaag ggatgccctg aaagatgtta tggttgaaat agcaaagaag
     6061 atagcaaggg atggggaagg agccagcaag cttatcgagg tagaggttac cggtacagaa
     6121 gacgaagctc aggctataag aatggcaaga gcggttgctg gtagcaatct ctttaaatcc
     6181 gcagtgtggg ggaaagagat aaactgggga aggattataa gtgcccttgg agctaattcc
     6241 gacccatttg atcccgatag aatatccata tattttggtg atgtaaaagt ggtggagaat
     6301 ggaatagaag tggaatttga taaaaatgtg gttctgaaga tattagatgg taaggaggtg
     6361 ttaataagga tatccgttgg agacgggtct ggacggggaa aagcatgggg ttgtgacctt
     6421 actccagagt atgtgtatat aaacggaagt tatctctcat gagtctggta ataaaattag
     6481 gtggaagtgt ggcagaaaat ccgtctgctc tagaaaaggt aataagaggt ataaaagata
     6541 tatcggacgg caatccattt gttcttgtcc atggaggggg gaaacagata accagttttc
     6601 tttcagagtt aggtatagag acatattttg tggatggaga gcgatataca gatagaagaa
     6661 cgatggaagt tgttgagatg gtcctcagtg gactggtgaa taagcaaata acgagtacac
     6721 tctgtcatat ggggataaaa tctatcggtt tgagtggtag agacctgaat cttataacag
     6781 tcaggcgcaa gatggaactt ggctatgtag gagaggtaaa atcggttgat gtggagccaa
     6841 tgaagaggct ccttgatgct ggatttaccc tggttctatc tccggtatcc gagactgagg
     6901 atggtcttcc ctgtaatgtg aacgccgatt ttgtggcgat agaagttggt aaggctctca
     6961 gggcaagcaa gctttttctt tttacagatg ttacaggggt tctcgatggg aataaaaata
     7021 ttatacccct gttagatgtg gagactgccc gggattttat agagaggggg ataataaggg
     7081 aaggtatgat accaaaagtc aggtctgcaa tctctgcgat atcggcaggg gtgaaggagg
     7141 tatggatagg taataaccct gtacagggga caataataag agggaaagat aatggacctt
     7201 aaaaaagagg aaagtttata tatagctcaa ctttataaga gatttccgtt aactattgta
     7261 aagggtgagg gggtatatgt ctatgatgat gaggggaata gatatcttga ttttgcaggt
     7321 gggatagcgg taaactcact cggttacgct catccaaaga taattgaagc cttaacttcg
     7381 caggcgagag aacttattca tatatccaat ctctattata caaagcccca ggtagaactg
     7441 gcgaagaaac ttgtacagct gacaggcttg gataaggtct ttttctgtaa cagtgggaca
     7501 gaggctatag aggcttctct caagttttcc agacgctggg gtaaggataa gggaaagttc
     7561 aagtttatat cagcagttaa ctcattccac ggcagaacaa tgggaagcct gtcggtaact
     7621 ggacagagaa aatatcagga gccgtttgag ccccttatag atggagttac atttataagt
     7681 tataatgatt cagatgctct aaatgaagag ttaaaaaaag gagactatgc ggctgtaatt
     7741 ctagaaccga tccaaggaga aggaggggta aaccctgcca gaagagaatt cctggagtta
     7801 gcaagatacc tctgtgataa atatgatact cttctcatat ttgatgaggt gcagaccggg
     7861 ataggaagaa ccggtgctat gtttgccttt cagaattttg gggttactcc agacatactc
     7921 gctctggcta aggggctcgg tggaggtttc cccattgggg caacgattat taatgataag
     7981 gttgctcaga cgctccacta tggagaccat gcatcaactt ttggtgggaa tcctctggca
     8041 acagctgtcg ccaatgctgt cctggaagag atagagaaaa ttctaccccg tataaaagag
     8101 gtgggaggtt tcctcgagga ttgtttaaag aagaagattt ccccactgga taacgtcctg
     8161 gagataagag ggattgggct tatatatggt atcgatgtaa aaacaaatgc aagagatata
     8221 tgcgagagat gtataaaaga gggattactt gtaatcacct gtgggaacaa cacggtgaga
     8281 cttgtaccac ctctgattat aaacgaacaa gagattgaaa aaggggtata tatattagag
     8341 aaggtattga aggaggaaaa ataataattg ggatttcttt tacttgaaga tggtacactc
     8401 ttggaaggag tttctttcgg tgcagaaggg gaatctattg gtgaggtagt ttttaataca
     8461 tctatgattg gctatccaga atccataact gacccttctt atagtggaca gattcttgta
     8521 ttcacatatc cactgatagg aaactatggg atacagtttg aagatagaga atcagataca
     8581 cctctcctga gaggtgttgt tgttaaggag tactgtaaga ctccaagcca ctgggggatc
     8641 gattatgata tagatagttt tctgaagaga tttgggattg taggactctc agatatagat
     8701 accagggctc ttaccagaaa attgaggatt tacggaacac tcaggggtat aatttcttca
     8761 gaagaatttc tccctgatag gaaagaagag ctgatgagta gaatattaag ctggcatatc
     8821 gaggactttg actttgtaag gtcctcctgt gttaaagagc ctgtgtatct tggtaatgga
     8881 agcgacagga ttgttcttat agactgtggg gtaaaacagg ggattgtcag atctttactt
     8941 gaaattggtt tccaggttgt tatggtccct gcctggttta ccagagaaga tatattaaac
     9001 cttgatcctg atggtgttct catctctaac ggtcctggag atccacagag actatcttat
     9061 ctggttgaga ctactagaga acttatagat gagaatgtcc ccctgtttgg tgtatgtctt
     9121 gggcatcaga ttctgggtct ggctcttgga ggcaccacat ataaactgaa atttggtcat
     9181 agaggtgcaa atcaacctgt aaaggattta attaccggaa gagtttatat aacctctcaa
     9241 aatcacagtt atgtgatgga ctctgaagga ttacctgaag atatagaaat aagttttata
     9301 aatctgaatg ataagacggt agaagggtta aagtctatca gaagacctat aatgagtgtt
     9361 cagttccacc ctgaggcgtc tcctggacct ttagacacca ggtttctttt tgaagagttt
     9421 ataaaggagt gtatgaagaa aaattatgcc gcttaggaag catattaaaa aggtactggt
     9481 tatcggttct ggtcctatag taataggaca ggctgctgag tttgactatg ctggaactca
     9541 agcctgtaaa tcattaagag aagaagggat aagtgttgtc cttataaaca gtaatcctgc
     9601 taccataatg accgatacaa tgatggcaga cagggtatac atagaaccca tagaggtaga
     9661 ggctatagag aaggttatag ctaaagagag acccgatgga cttctcccca cacttggagg
     9721 gcagacaggt cttaatatgg cagttaagct ttatgaagca ggtatactgg acaagtatga
     9781 ggtagaactt ctgggcacat cacttgatgc aataaagaag gctgaggata gggaattctt
     9841 taagaatttc attctgagta taggagaatc tgtcccggag agtgagatag taagggacct
     9901 ggaatcggca aggagttttg ctgagagagt tggtctaccc cttgtggtga gacctgccta
     9961 taccctgggt ggaactggcg gtggttttgt agagaaatgg gaagatttgg atgaggcgat
    10021 ctctgttgga ctcaatgcca gtatggttgg tgaggtcctg ttagagagaa gtgtaagagg
    10081 ctggaaagag atagagtatg aagtgatgag ggacagtggt ggaacctgta taactgtctg
    10141 tagtatggaa aactttgacc ctatggggat tcataccggg gatagtatag tggttgctcc
    10201 aagccaaaca ctgacaaaca aagagtatca gttattgagg
//
LOCUS       DAOTKU010000006         9561 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_7393_length_9561_cov_2.910267, whole genome shotgun sequence.
ACCESSION   DAOTKU010000006 DAOTKU010000000
VERSION     DAOTKU010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9561
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7393_length_9561_cov_2.910267"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1170
                     /locus_tag="P5310_00300"
     CDS             <1..1170
                     /locus_tag="P5310_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU31839.1"
                     /translation="EGFKSSYSRLNPDGMLLFTRWLQRPPTEELKLFNIILTGLRELG
                     IGDPENRIVAFRSLNTMTFIVKKGDLIYKEIEIIKRFTGSMSFDFVFLDGLTPIEANR
                     YNVLPDDIYYRYFQELFYNSNFSDIYPFRIDPPRDDKPFFFHFFKIDQIDAILKNWGH
                     TWQPFGGAGYIVIMTVLSLIILISFIFIILPFLIDKTKPPKTYRVSNFSFYFFVIGLA
                     YMFVEIPIMQRLILYLGKPVYSFSLTLSLLLIFSGIGSSLAIRLKKCPVLFILGSILL
                     LLSFLISSVIEKTLGYPFYCRVIISTVMIGLSGFLMGMPFPLALEKAKGHSVDIIPWC
                     WAINGFASVLSSFLSTILAIHIGFTAILAIGGTLYLLAGLILTLTPDKRNEQDVS"
     gene            complement(1132..1842)
                     /locus_tag="P5310_00305"
     CDS             complement(1132..1842)
                     /locus_tag="P5310_00305"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HRU31840.1"
                     /translation="MVFRYRVLIPVLILSFLFTLGLDSHEEEGYLKEAREKMVELIKT
                     RGITDKRVLAAMLKVPRHLFVDRSQWRSAYDDRPLPIGFGQTISQPYIVALMTESLNI
                     NMGDRVLEVGTGSGYQCAILAELTDKVYSVEIIEELGRKASMVLKELGYKVRIKIGDG
                     YNGWEEYAPFDAIIVTCAPDHIPTPLIQQLKVGGRMVIPVGPTGLYQTLWLITKEKED
                     KLVYKNLGDVLFVPLVRR"
     gene            complement(1857..3593)
                     /gene="thrS"
                     /locus_tag="P5310_00310"
     CDS             complement(1857..3593)
                     /gene="thrS"
                     /locus_tag="P5310_00310"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HRU31841.1"
                     /translation="MNSEKKTEVKEGFIELRHTSSHILAQAVKRLYPDAKLGIGPAIE
                     DGFYYDFYVDKPFTPDDLVRIEEEMKKIVDADYKLERFTLQKEDAIKFLEELDEPFKI
                     ELAREIDGEISFYRQGEFVDMCAGPHIESTGGVKYFKLLSTSGAYWRGDEHNPMMQRI
                     YGTSFYTKEELDNYLSFLEEAKKRDHRILGRELDLYSTHEEVGPGLIMWHPNGAIIRN
                     TIEDLWRKEHLKHGYKLVYTPHIASEKIYQISGHLENYAESMYAPIQIDTARYRLKPM
                     NCPGHILIYKTKQRSYRDLPIRYAELGTVYRYERIGTLHGMLRVRGFTQDDAHIFCRE
                     DQLLDEIRGVIRFMDFFMKTFRYEYKPYLSTRPEKYIGTDEIWDMATKALKEALRLEG
                     MDYELDEGGGVFYGPKIDIKLIDALGREWQGPTIQVDFNLPERFDVNYTGSDGKEHRV
                     VMIHRTVLGSMERFVGGLIEHYAGKFPLWLAPVQVVVIPISDRHNERAIEVKDRLFEN
                     NIRVELDDRNEKVNYKVREAIVKKVPYIVVIGDREIEDNTLSVRTISKDLGSFSLESF
                     IDRVKEEIEKKL"
     gene            complement(3664..4038)
                     /locus_tag="P5310_00315"
     CDS             complement(3664..4038)
                     /locus_tag="P5310_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU31842.1"
                     /translation="MREEELKILKMIEEGKITAEEGIKLLDAVREENTRDTGKFPGKR
                     IKISVVDKDAGRKKVDISVPLPLAKAFLKYGLVSGATFGGFSLKESGIDIDELQKMID
                     EGLEGTLIQVDDEGEKVEIKVE"
     gene            complement(4041..4421)
                     /locus_tag="P5310_00320"
     CDS             complement(4041..4421)
                     /locus_tag="P5310_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU31843.1"
                     /translation="MEILWIILAILSLGGAIFLLPLTLSTGIFWIVSGILVVLIIALL
                     VQLIVFLAPILFVLGVVLIIWGLIENPHKRVGIERRIEELVDKITGRSRDYERERLEI
                     LEMLERGEISYEEAMERIRQIKRR"
     gene            complement(4423..5334)
                     /locus_tag="P5310_00325"
     CDS             complement(4423..5334)
                     /locus_tag="P5310_00325"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HRU31844.1"
                     /translation="MNGQIPVEERISKIVVDSTGGDIDILSNKGNEISYEIGDRLEIE
                     SEVKNETLIIRIKGKGGIGIPFFNFGGWGDGDISLKIPQEKSLELNLKSGDISFSDYK
                     GNDIVLRGASGDISVSRIVCKNFSAHLTSGDVDIDIETKRGNINCRSGDVTVNLRGEE
                     WDVDISGVSGDVDVNTESGSIDLYISGISGDVTLNDKSIGEGKIGKKHLTIDGGRNKL
                     NINVISGDISIKTAKEVGESSFSQVTTLTEPSKSFLQERDTAAQYIPEEVKKVKEMYK
                     SGKISKEDAIGLIEAMGYGGLNNILEE"
     gene            complement(5331..5699)
                     /locus_tag="P5310_00330"
     CDS             complement(5331..5699)
                     /locus_tag="P5310_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012002862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 domain-containing protein"
                     /protein_id="HRU31845.1"
                     /translation="MNKIITKCPVCGEEMYIVSLRCPGCETEIKGTFYLDEFFKLSRE
                     QLNFIKLFIKNRGNLSDLGKELNLSYPTLRNRLNEIAKTLGYPVEEERVDRIEVLEKL
                     ERGEISSQEAIKLLKGGEER"
     gene            5867..6439
                     /locus_tag="P5310_00335"
     CDS             5867..6439
                     /locus_tag="P5310_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HRU31846.1"
                     /translation="MQQLIEIRWHARAGQGAKSAAYLLAESALGAGKYIQAFPEYGPE
                     RMGAPMKAYTRIGEAPIRIHSQITDPDVIVVLDPSLLKLSDTLEGWKDSSVLIINTNK
                     SPKEIRESLNLKGGKVCTIDATRISMDTIGRNIPNTPLLGALIRVMGTPSLDGVLEEL
                     RRKFSGRFSEKVMQGNIEAVKRAYEEVQIE"
     gene            6436..6720
                     /locus_tag="P5310_00340"
     CDS             6436..6720
                     /locus_tag="P5310_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRU31847.1"
                     /translation="MSLPGWKEMPEGIVILDAGNAQEYETGSWRTFRPVVDKGKCTNC
                     LFCWIYCPDTSVMVKDGKMVDFDYAHCKGCGICSKVCPVKAINMVGEGSL"
     gene            6717..7898
                     /gene="porA"
                     /locus_tag="P5310_00345"
     CDS             6717..7898
                     /gene="porA"
                     /locus_tag="P5310_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015907412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HRU31848.1"
                     /translation="MKTKNFIALEGNQAVALAMKQIDPDVVAAYPITPQTEIVQVFSE
                     YVANGNVHTEFVTVESEHSALSACIGSSAAGARVMTATSSQGLALMWEMLYIASGARL
                     PIVMTVVNRALSSPINIHCDHSDAMGARDSGWIQLWSETAQEAYDNLIQAIKIAESIN
                     LPVMVNMDGFITSHAEEMLEPLEDEDVKKFIGEYKPIRSLLDVEHPFTVGPLALQDSY
                     FEFKRSQAEAYKKVVPAVREAAEGFAKITGRKYDLFETYKLEDAEMGIIVLNSTAGTA
                     KEVVDRLREDGKKVGLLKPRLFRPLPVEDLKKTLSHLKVIGVMDRADTFGGFGGPLFG
                     DTRSVLYDVEHRPIIVDYIYGLGGRDVTMDQIYKVFEDLDFINRTGRVESLISYIGIR
                     E"
     gene            7910..8842
                     /locus_tag="P5310_00350"
     CDS             7910..8842
                     /locus_tag="P5310_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRU31849.1"
                     /translation="MPVNIKELSGKEDRLTGGHRLCAGCGASIAVRQILLAIDKPVVV
                     ANATGCLEVATTIYPYTAWKTPWIHSAFENAAATISGVEAAYQSLKRQGKITDDDIAF
                     LAFGGDGGTYDIGFQSLSGAMERNHNILYICYDNEAYMNTGIQRSSATPPGAWTTTTP
                     VGKVRPGKVGGKKDIVASIAEQGAMYVAQASISNFRDLIEKVRKAVSIDGATFINVLS
                     PCPRGWRTDTSDTVNIARLAVDTCYWPLYEVENGKWKINYTPRQKLPVTEYLKPQGRF
                     AHLFRPENQHILEEIQEWVDSKWDLLQKKAQLFQ"
     gene            8788..>9561
                     /locus_tag="P5310_00355"
     CDS             8788..>9561
                     /locus_tag="P5310_00355"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HRU31850.1"
                     /translation="MGRFQMGPSSEESSAIPVILAHRGASLLAPPNTLSSFKKAVELG
                     ADGVELDVRLTKDNKLVVIHDPVVDHVSDGKGFVSNYTLGEIKKLDFGYRFSEEFRGE
                     KIPTLEEVLDILRCLDLINIEIKNDPFPYRDIEKITLETIYNAGIKDKIIISSFDHEL
                     LVRIRELDKNIKTGVLYSFRPVYSNLLAIDARADNLHPFWAVIGEDTVRDAHRIGLNI
                     FPWTVDEKDIMERLMRWGVDGIITNDVQLGLRVRSEVFPS"
BASE COUNT         2605 a         2033 c         1826 g         3097 t
ORIGIN      
        1 gagggattca aatcttcata ctctcgatta aatcctgatg gtatgcttct ttttaccaga
       61 tggcttcaga gacctccaac ggaggagcta aagctattta atattatcct taccggttta
      121 agagaattgg gaattggaga tccggaaaat aggattgtcg cattcagatc tcttaatacg
      181 atgaccttta tcgtgaagaa aggagatctt atatataagg aaatagaaat cataaagaga
      241 tttacaggct ctatgtcctt cgactttgtt tttcttgatg gacttacacc gatagaggcg
      301 aatagataca atgtacttcc agatgatatc tactacagat attttcaaga gcttttttat
      361 aacagtaatt tctctgatat ttatcctttt cgtatcgatc caccaaggga tgataagcca
      421 tttttcttcc attttttcaa gatagatcag attgatgcca tattaaaaaa ctgggggcat
      481 acctggcagc cgtttggagg agcagggtat atagttataa tgactgtact ttctctgatc
      541 atcctgatct cattcatttt tattatactc ccattcttga tagataagac aaaaccccct
      601 aagacctata gggtctccaa tttttctttc tacttttttg taataggtct ggcatatatg
      661 tttgttgaga tccctataat gcagagactt atcctctatc ttggaaaacc tgtctattcc
      721 ttctctttga ctctatcatt acttcttatc ttttctggga ttggaagtag ccttgcaatt
      781 aggttgaaaa agtgcccggt gctctttatc cttgggtcta tactgttatt actctctttt
      841 ttgatatcct ccgtaataga aaaaacactg ggatatccct tttactgtag ggtaattata
      901 agtacagtaa tgataggtct ttcaggtttt cttatgggga tgccttttcc gttagcactg
      961 gaaaaagcca aaggtcactc tgttgatatt ataccctggt gttgggctat aaacggattt
     1021 gcgtcagttt tgagttcttt cctttccact atactggcta ttcatatcgg ttttacggct
     1081 atcctagcta taggtgggac cttatatctt ctggctggac tgatacttac actaacgcct
     1141 gacaagagga acgaacagga cgtctcctag atttttgtag acaagtttat cctctttttc
     1201 tttagttata agccataatg tctgatacag tcctgttgga ccaaccggga ttaccattcg
     1261 tccacctacc ttaagttgct gaataagggg cgttgggata tggtcaggtg cacaggtgac
     1321 aattatagca tcaaatgggg cgtactcctc ccagccattg taaccatcac caatctttat
     1381 cctgacctta taacccagct ctttcaaaac catactggct tttctcccca gctcttctat
     1441 tatctctaca gaataaactt tatcggtaag ttctgctagt atagcacatt gatatcctga
     1501 gcctgttcct acctctagga ccctgtctcc catatttata ttgagactct ctgtcataag
     1561 ggcaactata tatggctgag atatcgtttg tccaaaacct atagggaggg gtctatcatc
     1621 gtaagctgac cgccattgac tcctgtcaac aaatagatgc ctcggaacct taagcatagc
     1681 agctaagacc ctcttatctg ttatgcctct cgtctttata agctcaacca tcttttctct
     1741 tgcctcttta agatatcctt cctcttcatg tgaatctaat cccaacgtga agaggaagga
     1801 taaaattaac acaggtatca ataccctgta tctgaagacc aaactatcga acctcctcag
     1861 agttttttct ctatctcctc tttaactcta tcaatgaagg attctaggct aaaacttcca
     1921 aggtctttac tgattgtcct tacagagagt gtattatctt cgatctccct gtcgcctata
     1981 acaactatat atgggacctt tttaactata gcctctctca ccttatagtt taccttctca
     2041 tttctgtcat ccagttctac tcttatatta ttttcgaaga gtctatcttt tacttctata
     2101 gccctctcat tatgcctgtc tgatataggt attactacta cctggacagg ggcaagccat
     2161 agtgggaact tgcctgcgta gtgttctata agtcctccaa caaacctttc catcgatccc
     2221 agaactgtcc tgtgaatcat tactactcga tgctctttac catcacttcc tgtatagttt
     2281 acatcaaatc tttccggaag attgaagtct acctggatag ttggtccctg ccattctcta
     2341 cccagtgcat ctatcaactt tatatcaatc tttggtccgt aaaagactcc tcctccctca
     2401 tccagttcat aatccatacc ttccagcctg agcgcttcct tcagtgcttt tgttgccata
     2461 tcccatatct cgtctgttcc tatatatttc tctggtcttg tagagaggta gggtttatat
     2521 tcatacctga atgtcttcat aaagaagtcc ataaacctta taacccctct tatttcatcg
     2581 agaagctggt cctccctaca gaatatgtgg gcgtcatcct gggtaaaacc ccttaccctt
     2641 aacataccat gtaatgtccc tatcctctca tatctgtata ccgttcccaa ttcggcatat
     2701 cttatcggta agtctctgta gcttctctgc tttgtcttat agatgagtat atgacccgga
     2761 cagttcattg gtttgagtct atatcttgca gtatctatct gtatgggtgc atacatagac
     2821 tcggcatagt tttcgagatg tccacttatc tggtatatct tttcactggc tatatgggga
     2881 gtatatacaa gtttataacc gtgcttgagg tgttctttac gccacaggtc ctcgatagta
     2941 tttcttatta ttgccccatt gggatgccac atgataagtc caggaccgac ctcttcgtgc
     3001 gtactataca ggtcaagttc tctaccgagt attctatggt cacgtttctt tgcctcttct
     3061 aggaatgaaa gatagttatc aagttcttcc ttagtataga aacttgtacc atatatcctc
     3121 tgcatcattg gattatgctc atcacctctc cagtatgcac ctgaggtgga gagaagttta
     3181 aaatacttta cccctcctgt actctctata tgtggtccag cacacatatc tacaaactct
     3241 ccctgtctat agaaggagat ctcaccatct atctctctgg ctaactctat cttgaacggt
     3301 tcatctagct cttctaagaa ttttattgcg tcctcttttt ggagagtaaa tctttccagc
     3361 ttataatctg cgtcgacaat ctttttcatc tcctcttcta tacgcaccag gtcatccgga
     3421 gtaaatggtt tatccacata aaagtcgtag taaaacccat cctctatagc tggacctata
     3481 cccaatttgg catcaggata gagcctcttt accgcctggg caagtatatg actggatgtg
     3541 tgtcttaact ctataaatcc ttctttaact tctgtcttct tctctgagtt cataaaagcc
     3601 tccttatgaa aattataaag ccgtaacaat aattatatta tatctctatc tttaaaatag
     3661 tatctactcc acttttatct cgaccttttc tccctcgtcg tctacctgta taagcgtacc
     3721 ctctaatccc tcatctatca tcttctggag ttcatctata tctatcccgg attctttcaa
     3781 actgaatcct ccaaaggttg ctccactgac cagtccatat ttaagaaaag cctttgccag
     3841 cggaagtgga actgagatat ccaccttctt tctgccagca tccttatcta ctacagatat
     3901 ctttatcctt ttaccgggga attttccagt atccctggta ttctcttccc ttactgcatc
     3961 taacaatttt atcccttctt ctgctgttat cttaccctct tctatcattt taagtatctt
     4021 taattcttcc tctctcatct ttatctcctc ttgatctgcc ttattctttc catagcttcc
     4081 tcataactga tctctcctct ttccagcatc tccagaatct ccaatctttc tctctcataa
     4141 tccctgcttc taccagtaat cttatctacc agttcttcta tccttctctc tatccccacc
     4201 ctcttatgag gattttctat cagcccccat attatcagta ccactcccag aacaaataat
     4261 attggtgcta aaaagacgat aagctgtacc agaagagcga tgattaagac gactaaaatt
     4321 ccgctcacaa tccagaatat tccagtgctt agggttagag gtaaaagaaa aatagctccc
     4381 cctaaagata gtattgctaa gattatccac agtatttcca tcttactcct ccaatatatt
     4441 atttaatcca ccatatccca tagcctctat aagacctatg gcatcctcct tagatatttt
     4501 accagattta tacatctctt tcaccttttt tacctcttct gggatatact gtgccgctgt
     4561 atctctctct tgaagaaatg attttgatgg ttctgtaaga gttgtaacct gactgaaact
     4621 gctttctcct acctctttag cggttttaat tgatatatct ccagagatta catttatgtt
     4681 cagtttattc cttccaccat ctattgttaa atgtttctta ccgatttttc cttctcctat
     4741 agacttatcg ttcaaagtta cgtctccact tatgccgctt atatagaggt ctatcgaacc
     4801 agattcagtg tttacatcta cgtctccgct tacccctgag atatccacat cccactcttc
     4861 tccccttaag tttaccgtta catccccgga cctgcaattt atgtttcccc ttttggtctc
     4921 tatatcaatg tccacgtcac cacttgtgag atgggcagag aagtttttac atacaatcct
     4981 gctgacagat atatcccccg atgcacccct taatactata tcattaccct tataatctga
     5041 aaaagatata tcccctgatt ttagattcag ttccagggat ttctcctgcg gtattttgag
     5101 tgatatatct ccgtcccccc atcctccgaa gttaaaaaat gggattccaa tgcctccttt
     5161 acccttgatc cgtattatca acgtctcatt tttaacttca ctttctatct ctaatctatc
     5221 acctatctcg taagatatct cgttaccctt attggatagt atatctatat ctccacctgt
     5281 gctatctacc actatcttgc ttattctctc ttctaccggg atttgcccgt tcatctttct
     5341 tctcctccct tcaatagttt aatggcttct tgagaactga tctctcctct ttctagcttt
     5401 tccagtacct ctatcctgtc taccctttct tcttccactg gatagcccag tgttttggct
     5461 atctcattca gtctatttct taaagttgga taggatagat ttagctcctt tcccagatca
     5521 ctcagatttc ctctattctt tatgaataac tttatgaaat ttagctgctc tctggagagt
     5581 ttaaaaaatt catccagata gaaagtccct tttatctctg tttcacagcc cgggcatctg
     5641 agtgagacta tatacatctc ttccccacag actggacatt tcgttataat cttgttcatt
     5701 ttagcctcct ttcatttttt gaaattatat attaaattat ttaaaatgtc aataaaattt
     5761 tctgcatatt agattaactt ctctgtttga caaaaaaacc ttcttatgtt atagtttatg
     5821 aggattttac agcgttctct aatatttatt aagaaggagg ttgcatatgc aacagcttat
     5881 agagatacgt tggcatgcaa gagctggaca gggagccaaa tctgctgcct atcttcttgc
     5941 cgagtctgct cttggtgccg gtaagtatat acaggctttt ccagagtatg gtccagagag
     6001 gatgggtgct cctatgaagg cttacaccag gataggagaa gcccctataa gaatccattc
     6061 tcagattacg gacccagatg taatagtcgt tttggatccg agtctcctca agttaagtga
     6121 tactctggag ggctggaagg attcgagtgt acttataata aataccaata aatctccgaa
     6181 agagataaga gaaagcttaa atttaaaggg aggaaaagtc tgcactatag atgcaaccag
     6241 aatatcgatg gataccatag gcagaaatat ccccaacacc cctttacttg gtgctctaat
     6301 aagggttatg ggtacccctt cgctggatgg agtcctggaa gagttgaggc gtaaattttc
     6361 aggcagattc agtgaaaaag tcatgcaggg gaatatagaa gcggtgaaac gtgcttatga
     6421 ggaggtgcag atagaatgag tcttccaggc tggaaggaga tgccagaagg tatagtaatc
     6481 ttagatgcag gaaatgcgca ggaatatgag acaggttcgt ggagaacatt ccgacctgtg
     6541 gtagataaag ggaagtgtac aaactgtctt ttctgctgga tatactgccc agacacatca
     6601 gtgatggtta aagatgggaa aatggtagac tttgattatg cccattgtaa gggctgcggt
     6661 atatgcagta aggtatgtcc ggttaaggct ataaatatgg tgggggaggg aagcttatga
     6721 agacgaagaa ttttatagct ctggagggta atcaggctgt cgcattggct atgaagcaga
     6781 tagaccctga tgttgttgct gcctatccaa tcactccaca aacggagata gttcaggtat
     6841 tctcagagta tgttgctaat ggtaatgtcc ataccgagtt tgtcaccgta gagagcgaac
     6901 attctgctct ttctgcctgt ataggttctt cagctgctgg agccagggtt atgactgcca
     6961 cttcttctca gggtttagcc ctgatgtggg agatgctata tatagcttca ggtgccagac
     7021 taccaatagt gatgactgtt gtaaacaggg ctttaagctc tcctataaat attcactgtg
     7081 accattctga tgctatgggc gccagagata gtggctggat acaattatgg tcagagacag
     7141 cacaggaagc ttatgataat cttatccagg cgataaagat agcggagagt attaatttac
     7201 cggtaatggt aaatatggac ggatttatta caagccatgc agaagagatg cttgagcctc
     7261 tggaagatga agatgtgaaa aaatttattg gtgaatataa accaatcagg tctcttcttg
     7321 acgttgaaca tccatttacc gtaggaccat tagctcttca ggattcttat ttcgagttta
     7381 aaagatcaca ggcagaggct tataaaaaag ttgttcctgc ggtaagagaa gctgctgaag
     7441 gatttgctaa aatcacagga cgtaagtatg atctatttga aacctacaag cttgaagatg
     7501 cagagatggg tataattgtt ttgaactcta ctgcaggtac agctaaagag gttgtggacc
     7561 ggcttagaga ggatggtaag aaagttggac ttcttaaacc aagacttttt agacctcttc
     7621 ccgttgaaga tttaaagaag acgctctctc accttaaagt tattggtgta atggacagag
     7681 cagatacatt tggaggtttt ggtggacctc tcttcggcga tacaaggtca gtcctctacg
     7741 atgtggaaca tcgacctatc atagttgatt atatctatgg acttggtgga agagatgtta
     7801 ctatggatca gatttataaa gtttttgagg atttagactt tataaataga acaggaagag
     7861 ttgaaagttt aatcagttat attggtatta gagagtaagg aggtctaata tgcctgttaa
     7921 tataaaggaa ctttctggaa aggaagatag gctcactggt ggacacaggc tttgtgccgg
     7981 ttgtggagca tctatagcgg tgaggcagat actccttgcc atagataaac cggttgtggt
     8041 agcaaatgcc acaggatgtc ttgaggttgc taccaccata tatccatata cagcatggaa
     8101 gacgccatgg attcacagtg catttgaaaa tgcggctgcc acgataagcg gtgtagaggc
     8161 agcttatcag agtctcaaga gacaggggaa gataactgat gatgatatag ccttcctcgc
     8221 ttttggagga gatggcggaa cctatgatat aggttttcaa tctctttctg gagctatgga
     8281 gagaaatcac aatatactct atatatgcta cgataatgag gcgtatatga atacaggtat
     8341 ccagaggtcc agtgcaactc ctcctggagc ttggactaca actacacctg taggcaaagt
     8401 aagaccaggt aaggttggag ggaaaaagga tatagttgcc tctatagcag aacagggggc
     8461 gatgtatgtg gctcaggctt ctatctctaa ctttagagac cttatagaaa aggtgaggaa
     8521 agctgtttcg atagatggcg ccacattcat aaacgttctt tcaccctgtc ctaggggatg
     8581 gaggacagat accagtgata ctgtgaatat agccagactg gcggtggata catgctactg
     8641 gcctctctat gaggtagaga atggtaagtg gaagataaac tacactccaa gacagaaact
     8701 gccagtaact gaatatctta agcctcaggg aagatttgct cacctattta gacctgagaa
     8761 tcaacatatc ctggaagaga ttcaagaatg ggtagattcc aaatgggacc ttcttcagaa
     8821 gaaagctcag ctattccagt aatattggca cacaggggag ccagtctact ggctccccct
     8881 aatactctgt cttcattcaa gaaagctgtg gaacttggag cagatggagt agagcttgat
     8941 gtgcgattaa ctaaggacaa taaattggtt gtgatacatg accctgtggt agaccatgtc
     9001 tctgatggaa aaggttttgt gtcaaattat actcttggag agataaaaaa attggatttc
     9061 gggtatcgct tctctgagga gtttaggggt gaaaagattc ccacattaga agaagttctg
     9121 gatattttga gatgtcttga tcttataaat attgagataa aaaacgaccc ctttccttac
     9181 agagatatag aaaagataac cctggaaact atttataatg cagggattaa ggataaaatt
     9241 attatatctt cttttgacca tgagttactt gtaaggataa gagagctgga taaaaatata
     9301 aagacaggtg ttctttattc ttttagacca gtttattcta atttattagc catagacgca
     9361 agagcagaca atcttcatcc cttttgggca gtgataggtg aagacactgt gagagatgcg
     9421 cataggattg gtctgaatat atttccatgg actgtagatg aaaaagatat tatggaaagg
     9481 ctgatgagat ggggagtcga tgggataata accaatgatg ttcagttagg tctgagagtt
     9541 aggtctgaag ttttcccttc t
//
LOCUS       DAOTKU010000007         9245 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_7652_length_9245_cov_3.361262, whole genome shotgun sequence.
ACCESSION   DAOTKU010000007 DAOTKU010000000
VERSION     DAOTKU010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9245
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7652_length_9245_cov_3.361262"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1567)
                     /locus_tag="P5310_00360"
     CDS             complement(<1..1567)
                     /locus_tag="P5310_00360"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HRU31851.1"
                     /translation="MYTEVPLLVLAGAGSGKTRVITYKIAYLVKELDIFEDNILGVTF
                     TNKAANEMKTRVARLLGKSVSDVWLGTFHSIAVKILRRYSQYIGINNSFSILDEDDQT
                     SLMKEVISELNLDIKRYPPGPFIDRISKAKESLIDPVTFGEFVESYYESIVAKVYQRY
                     QEKLMRNQALDFDDLLMNLVKLFRENERILSYYQNKFRYILVDEYQDTNYAQYMIVFL
                     LAQAHRKLTVVGDDDQAIYSWRGADIRNILEFEKDFPGAKVIRLEQNYRSTQNILDAA
                     NKVIAHNRFRKGKNLWTNNERGNRIKLYIAKDEIAEANFIAREIILLQKREGLNFGDF
                     AVFYRTHAQSRALEEAMIKNGIPYRIIGGVKFYQRKEIKDILAYLKFIVNQYDGISFK
                     RIVNVPRRGIGGATLSKITDYATEKNISYLEAMKELNQKDEFGKKIEKEIDKFIQLIE
                     DLTGFSKIHSVEELIIEILDKTGYIKELTELQTATAQVRIENVEELISVAKEFIKNTG
                     DTGLEGFLNYISLI"
     gene            complement(1625..2911)
                     /locus_tag="P5310_00365"
     CDS             complement(1625..2911)
                     /locus_tag="P5310_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012411933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/sulfonate/bicarbonate ABC transporter
                     ATP-binding protein"
                     /protein_id="HRU31852.1"
                     /translation="MAIIETKNLTIIFDMPNNTRITVVEDINLSLEEGEFVSILGPSG
                     AGKSTLLRAIAGLIKPTHGEVLYRGELVEGVNPGISMVFQTAALFPWLTVLENVELGL
                     KAKGTPPKERRERALKMIDLIGLDGFESAYPKELSGGMRQRVGFARALVVEPEVLLMD
                     EPFSGLDVLTAENLRNDLLELWIEKRIPTKTILLITHSIEEAVYLSDRVIVLDKNPGR
                     IKQEIYINIPHWRDRESEEFIRFVDRIYSIMMGKPITAKPGRPEKYGIPLPHVRVGAI
                     AGFVELIAELEEKEDIYKIAGDLNMDLADIQPVIEACVLLKFIDTTGGDISVTKEGEI
                     FAEADVLEKKEIFKKQILANVPFIQQIIQVLNNKSNHRMPVEFFIELLETKFSTSEAQ
                     AQMEIAIDWGRYAELFAYDEDSKEVYLEELLESEEV"
     gene            complement(2924..4627)
                     /locus_tag="P5310_00370"
     CDS             complement(2924..4627)
                     /locus_tag="P5310_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRU31853.1"
                     /translation="MVYRIVPPKRIKISIIDIGIILFLSAFIYGIISTAKDWGEIPVY
                     ALNISLSPVNLPYYAWLSVLRQFLAYLLSLGFSITYGYVASHNKRLEPIMISLLDILQ
                     SVPVLSFMPGVILGLMALFPYGRVGIELGSIVLIFTGQVWNMAFSVYSSMSSLPREFR
                     EASEIFNLTPLQRFLYVELPYSTIGLVWNSMMSVAGGWFFLMACEMFTLGERDFRLPG
                     LGSYLQLAAIEGDTEAVLMGLATMIAIIVIIDQLVWRPLIAWSQKFKVELATEEIIPT
                     SIVLTVIERSTLILKIGGVINTLMEKISAGFSRTTPRKSRGKSPFIFILRIIIIGILA
                     LILGYASIRLFLLISGIPVNKWGEIMINDLFTLFRVSIAVVLSILWTLPLGVFIGTNP
                     RLSSVMQPLVQILASIPATAVFPIILMWLLSMRGGLSIASILLMLLGTQWYLLFNIIA
                     GAMSIPADLKEAADVFQLKGVERWRKLIIPAVLPYLVTGGITATGGAWNASIVSEYVH
                     FRGQILTVRGLGAMISSSADKGDISLLAGSTIVMIITVTLINRFFWRRLYWLAEEHYS
                     L"
     gene            complement(4676..7774)
                     /gene="ebgA"
                     /locus_tag="P5310_00375"
                     /pseudo
     CDS             complement(4676..7774)
                     /gene="ebgA"
                     /locus_tag="P5310_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017798056.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
     gene            complement(8063..8359)
                     /locus_tag="P5310_00380"
     CDS             complement(8063..8359)
                     /locus_tag="P5310_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU31854.1"
                     /translation="MTEFKSSRENLRKMGLNERQVKAVMYVKEKGRITNKEYQNVANV
                     KERLATIELNNLVEKKILQKFGTTGRGTYYTALKAQKPQERRTEGAKKGDKGAR"
     gene            complement(8356..>9245)
                     /gene="radC"
                     /locus_tag="P5310_00385"
     CDS             complement(8356..>9245)
                     /gene="radC"
                     /locus_tag="P5310_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HRU31855.1"
                     /translation="FMISNAIYPFYSKNGKVFLKCSRRGLLDQEKRDEIIQQLNIKTL
                     EECEELRERIKKEVAGRRVSRPIKEWIEEERPREMLVRLGAENLPLSKLLAIILRTGK
                     EGMSAEEIAKILLNHFGSLRAIDSAPISELSKIEGVGFAKTVQIKAALELGKRFFKEK
                     ANARKKLKRPDDVIDYVSEYYAPYLRDKEKEFFSVILLDIKSKPIDNIEISKGSVNAT
                     VVDPKEIVKSASLKSASSIILVHNHPSGETTPSQDDIELTNRIVSACEMIRVKVLDHI
                     IIGKNTEDFYSFAKEGLIR"
BASE COUNT         2464 a         2192 c         1478 g         3111 t
ORIGIN      
        1 ttataagtga tatgtagttg agaaaacctt ccagacctgt atctccagta ttttttataa
       61 attcttttgc cactgagatt aattcctcta cattctctat acgtacctgg gcagtagcgg
      121 tttgcagttc tgtcagttct tttatatatc cagttttatc cagtatctct ataataagct
      181 cttctacaga atgtatcttc gagaatccag tcaagtcctc tattaattgg ataaatttat
      241 caatctcttt ttctatcttc ttcccaaact catccttttg attcaattcc ttcattgctt
      301 ctaaatagct tatattcttt tctgttgcat aatctgtaat cttagagaga gttgcacccc
      361 ctatacctct tctgggaaca ttaactatcc ttttaaaact tatcccatca tattgattca
      421 cgataaactt cagataagca agtatatctt ttatctcttt tctctgataa aattttacac
      481 caccgatgat tctgtatggt attccattct taatcatagc ctcttctaaa gccctcgatt
      541 gtgcgtgtgt cctgtagaat acagcgaagt ctccaaaatt caacccttct ctcttctgta
      601 agagtataat ctccctggct ataaaattag cttcagctat ctcatcttta gctatataga
      661 gctttatcct gtttcctctc tcattatttg tccagagatt tttccccttt ctgaatctat
      721 tgtgtgctat aactttattt gctgcatcca ggatattctg ggtagacctg taattctgct
      781 ccaatcttat aacttttgct ccagggaaat ccttctcgaa ctccaggata tttcttatat
      841 ctgctcctct ccagctatat atagcctggt catcatctcc aactactgtg agttttcgat
      901 gtgcctgggc aagcaggaat actatcatat actgggcata gttagtatcc tggtactcat
      961 ctaccaggat atatctgaac ttgttctgat agtatgataa tattctttcg ttctccctga
     1021 agagttttac cagattcata agcaggtcat caaaatccaa tgcctgattt cgcattaatt
     1081 tttcctgata tctctgatag accttagcca ctatactttc atagtagctt tccacaaact
     1141 caccaaaagt cacagggtct atcaggctct ctttagcctt acttatccta tcaataaaag
     1201 gtccaggagg atatcttttt atatcgaggt ttaactctga aattacctct ttcattaaag
     1261 aggtctggtc atcctcatcc agaatagaga agctgttatt tatgcctata tactgggagt
     1321 accttctgag tatctttacc gcaattgaat ggaatgtccc aagccataca tcgctgacac
     1381 ttttacccaa cagtctggct actctggtct tcatctcatt agcagcttta tttgtaaatg
     1441 ttactcccaa tatgttatct tcaaagatat ccagttcttt aaccagatag gcaatcttat
     1501 aggttattac cctggttttt ccgcttccag ctccagccag gacaagaaga gggacttcag
     1561 tatacatcac tgcctccctc tgttgaggat ttaattcatc caaaaaattc atatttcttt
     1621 tttattacac ctcttcgctt tccagcaact cttccagata cacctcttta ctatcctcat
     1681 cataggcaaa aagttctgcg tatcttcccc agtctatggc aatttccatc tgagcctgag
     1741 cttcactggt gctgaattta gtttcaagca gctctatgaa gaactccact ggcattcgat
     1801 ggttagattt attattgagg acctgaatta tctgctgaat aaaggggaca ttagccagga
     1861 tctgcttttt aaatatttct tttttctcca gcacatcagc ttcggcaaat atctccccct
     1921 ctttggtcac agatatatct ccacctgtgg tatctatgaa ttttaatagt acacaagctt
     1981 ctataacagg ctggatatct gcaaggtcca tatttaaatc tcctgctatt ttatatatat
     2041 cttctttctc ttccagttcg gctataagtt ctacaaaacc agctattgca ccgactctga
     2101 catgaggaag aggaatgcca tatttttctg gtctgcctgg ttttgcagtt atgggtttac
     2161 ccatcataat gctgtatatg cggtcaacaa acctgataaa ttcttcagat tctctatctc
     2221 tccagtgagg aatatttata tatatctcct gctttatcct gcccgggttt ttatcgagga
     2281 cgataactct atctgataaa tacacagctt cctctatact atgagttata agaagtatcg
     2341 tctttgttgg aattcgcttc tctatccata attccagaag gtcatttcta agattctctg
     2401 cagttaatac atccagccca gagaatggtt catccataag caaaacttct ggttccacca
     2461 ccagggctct ggcaaatcca actctctgcc tcattccacc tgagagctct tttggataag
     2521 cactttcaaa tccatccaga cctatcaggt ctatcatctt taaagccctt tctctacgtt
     2581 ccttaggagg ggtcccttta gcctttaatc cgagttccac attctctagc actgttagcc
     2641 agggaaatag agccgctgtc tgaaagacca tggagatacc tggatttacc ccctctacca
     2701 gttcacccct gtacagtact tctccatgag ttggttttat aagtcctgct attgctctca
     2761 gtaatgttga ctttccagct ccagaaggac caaggataga aacaaactca ccctcttcga
     2821 gggagagatt tatgtcttca actacggtta ttctagtatt attcggcata tcaaagatta
     2881 tagttaaatt tttagtctct ataatagcca tagtcttatt atatcacaat gagtaatgtt
     2941 cctctgcaag ccagtataat cttctccaga agaatctatt tatcaatgtt actgttataa
     3001 tcatcacaat ggtgctacct gcgagaaggc tgatatctcc tttatcagcg gaactgctta
     3061 tcattgcccc aagccctctt acagttaata tctgccctct gaagtggacg tattcagata
     3121 ctatagaggc attccatgca ccaccggtag cggtaatacc tcccgttacc agatagggta
     3181 gaacggcagg tataatcaat tttctccatc gctcaacccc ctttagttgg aagacatctg
     3241 ccgcctcttt taaatctgcc gggatagaca ttgctccagc tatgatattg aataatagat
     3301 accactgagt tcctaatagc ataagcaata ttgatgctat tgataatccc cctctcatac
     3361 ttaataacca cataagtatt attggaaata ccgcggtagc aggtatagac gccaggatct
     3421 ggaccagtgg ctgcatcacg ctggataacc ttgggttcgt cccaatgaag acacctaaag
     3481 gcaatgtcca aagtatagag agaactactg ctatgcttac tctgaacagg gtgaagagat
     3541 catttatcat aatctctccc cacttattga caggtatccc tgatataaga agaaacaatc
     3601 ttatagaagc ataaccaagt ataagagcta atatccctat aattataatt cttaaaataa
     3661 aaataaaagg agacttacct ctactttttc ttggtgtggt tctggaaaat ccggctgata
     3721 tcttttccat aagagtattt attaccccgc caatcttcaa tattagcgta gacctctcta
     3781 ttactgtaag gactattgag gttggaatta tttcttcagt tgccagttcc accttaaatt
     3841 tctgtgacca ggcaattaat ggacgccata caagctggtc tattatcaca ataatagcga
     3901 tcatagtggc taaccccata agtacagctt ctgtgtcgcc ctctatagct gctaactgga
     3961 gataagaacc taatccagga agtcgaaaat ctctctctcc aagggtaaac atttcacaag
     4021 ccatcaggaa gaaccagccg cccgctacag acatcatact gttccagacc aatcctattg
     4081 ttgaataggg taattcgaca tatagaaatc tctgaagagg tgtaagatta aatatctcag
     4141 aagcctctct aaattctctc ggtagagagc tcatagaact gtaaacactg aaagccatat
     4201 tccatacctg tccggtgaat atgaggacta tactgccaag ttctatcccc accctgccat
     4261 atggaaagag agccataagc cccagtatga ccccaggcat aaacgagagg accgggacag
     4321 attgcaggat atctaaaagg gatatcataa tcggctctaa tctcttatta tgactcgcca
     4381 cataaccata cgttatggag aatcccagag agagcagata agctagaaat tgtcttaaaa
     4441 ctgaaagcca tgcatagtat ggcaggttaa ccggggaaag agatatatta agggcataaa
     4501 caggtatttc tccccaatct ttagcagtac ttataatccc atatataaat gcggagagaa
     4561 acagaattat accaatatct attatcgata tctttatcct tttagggggt actattctat
     4621 agaccaatta gctatctcct gttatttttt aaacctgtct attatatcat aaactttaga
     4681 taacgtactt actcagctgg actgcggttg ttagagaatt tgagaatggt cttatcctta
     4741 aagaaaaatc gaacttctcc gggaataaga gatatttctc taacggagat ggtccacagc
     4801 ttgcactacc cagtccattg tgcttataat ctatattcat cactatataa tccctgggaa
     4861 tcaaatcaca ggcatgtttg gctttctcga agtcttccgg ttcaaatctg tgggcactga
     4921 agtttataaa tggcatccct gcgaatagta atccaatacc tcttctatct gttattgata
     4981 cccacttcac atctgtcctg tttccatttt cttgaggtct cacataaggg gtatagagtt
     5041 catctatatt ctttctgtat atcccaaaag gatttgcctc tttactatca atatatgatt
     5101 ctccaggtcc ccgtccatac cacattacct gttctaattc tttattcagt ctcatcttta
     5161 aacctatcct tggaaatacc atcggtagat tcccctgtgg tctcccttta acttccagga
     5221 tgatatcgcc acttccatag atgctgtaga tatactctgt atctatcttc cagtcaacaa
     5281 ccggtggggc tacacaggat ataacatcta ttcttatcag gttattgttt attaattcta
     5341 atgatacatc atctatcctg tgatttaata gacgtacgaa ctttttcgtc caatcctcta
     5401 tagcataccg gtcattgtcg ataggagctc tccacaggtt caatcttgga cctgtcttaa
     5461 tgagttctac accgctatac tggagactct ctatgattcc ccttacctta tcaaataaca
     5521 gtttaaattc tgctccttta acttcaatag tattatcaaa gtctctacag gtcaatggtg
     5581 gcatactggc aacactgata ctcttcggct ttttcctgga ttcaacagga agtttaaact
     5641 gagcccaggc aacggtatgc cctttttctc cccagttagt actgtttgca aggataaaat
     5701 gtatattcag ccagtagtct gttcctggtt ctggattatc tggtatcctg taaggtactg
     5761 taactaccct gcttgtcctg gcaggtaccc cttttataga tacttctcca cattcgataa
     5821 tctttccgtc tgcccatatc tcccaggaca ttatcagatg gtccagagag atgaattcat
     5881 acaggtttgt tatctttacc tctccttttg ttagatctat ctcttccacc ttcaccggtt
     5941 ctatcacttt cttatactct atcagtcctg gactgggtgt atggtccggg aataccagtc
     6001 catctattac aaagttccca tcgtttggct catctccaaa gtctcctcca taaccgaagt
     6061 attctttacc atctggtgtc ttcttcctta tcccgtggtc catccattcc catacacatc
     6121 caccctgaag tctcttatat ttataaaaaa cctcccagta ttctttcagg tttcctggtc
     6181 cattccccat tgcatgggca tactcgcata gtatatgtgg ctttggcata tctgtcatct
     6241 taccaagctc ttccaatttc tccactggtg tatacattgt gcttacaatc tctgatacct
     6301 tagcctgagt atccctttca tagtgtacca gtcttgttgg atcgttcttc ttgcaccact
     6361 cagccatagc ttcatggttt atcccgaatc cagactcgtt ccccagtgac cagaatatta
     6421 cacagggatg attcttatct ctgttcactg ttcattctat tctgtctatg tacgcctcct
     6481 gccattctgg atcgttgctc agtctattta tatctcctgc aaaggcaaaa ccgtgacatt
     6541 ccaggtctgc ttcatctatt acatagatcc catatctatc acagagatca tagaatctcg
     6601 ggtcatctgg atagtgggat gttcttaccg tatttatgtt gtgtcttttc atcaagatta
     6661 tactctgttc tgtccactct attggaactg ctcttccaaa atctggatga tgctcgtgtc
     6721 tatttactcc ttttaacttt ataggaactc cattaaccag aaatactccg tctctcagtt
     6781 caactttcct aaaaccagct ttaccgggaa tcacttcggc aacattacaa ccctcatctt
     6841 tcaatgtgag gataacatta tacaggtacg ggtcttctgc cgaccacttc ctcggattat
     6901 ctacaggaat ctctatctca aaagatttct cttcatctgg atccaatgtt atatccctta
     6961 tctctttctc cccaactggg ttcaggtcag tatccagcag agagaccgat agactgtatc
     7021 cttttacagc agtaccctcg gtatttacta actctactct ggtttttaac attccagatt
     7081 cacatctctc gtccagatcg gctactgtaa aaaagtctct tatatatgta tctggtctta
     7141 ttacaaggta tacatctctg aagataccac ttagccacca catatcctgg tcttcaaggt
     7201 aactaccatc tgaccactgg aataccatta ctgccactgt attcttccct gtccttacag
     7261 ctgatgttat gtcaaactct gatggaagcc tgctaccctg gctataacca acctcttttc
     7321 catttatcca tacgtagaat gcgctgtcca ccccttcaaa tctgagaatt attctcttat
     7381 ctttcaaatc ttcatcggca agatagaaat cccttctgta agaaccggtt ggattttccg
     7441 atggtaccct tggagggtct acaggaaaag gatatataac atttgtatag tgcggatgtc
     7501 catacccctc catctgccaa ttactgggaa ctcttatttc atcccaatca tctacattgt
     7561 agtcctcact ataaaatccc tgtgggactt cgagagggga agaggcatag taaaatttcc
     7621 atttcccatt taacatcctt atcctttcac ttgtctctct ttgaaaggtt aaagcgctat
     7681 ccacatcagg ataggggata aaataggctc tactctttaa cctgttcttc tctaatacct
     7741 tttgattctc ccaatcattt gttttcttct ccatattaac ctccagattc ttattataat
     7801 ggtaatattt tatcagattt tggcaagatt aatagagagg actttgtggg ataaccagac
     7861 aatattaaca aaggatataa atgcgcagag agtactctcc tgtaaaatgt cgttgacgca
     7921 tcagaggagc gcttaagatt tttagggcga gaaggattta taacacacac ctcccctagt
     7981 ctcacccatc gccagccagg cggtagtttg tatagctcgt taatgctatt tccactccct
     8041 gttttttgcc tccaggctaa attcaccttg ctcctttgtc accttttttt gcgccttcgg
     8101 tgcgtctttc ttgaggcttt tgcgccttta gtgcggtata ataagtacct cgccctgtcg
     8161 taccaaactt ctgtaatatt tttttctcaa ctaaattgtt aagttctata gtggctaatc
     8221 gctcttttac attcgccaca ttctgatact ctttattggt aatcctccct ttttccttca
     8281 catacatcac tgccttaacc tgtctttcat tcaatcccat ttttcttaaa ttctctctcg
     8341 agctcttaaa ctcggtcatc ttatcagccc ttcttttgca aaactataaa agtcctcagt
     8401 gtttttaccg atgattatat ggtctaatac cttcactcta atcatctcac aagcagacac
     8461 aatgcgattc gttaattcaa tatcatcctg agatggtgtg gtctctcctg aaggatggtt
     8521 atggacgagt attatggaag atgcagattt caatgatgct gatttgacaa tttccttcgg
     8581 gtcaacaaca gttgcattta cactgccttt gcttatctca atattatcaa ttggtttact
     8641 tttaatatcc agaagaatta cactaaaaaa ctccttttct ttatccctca aatagggagc
     8701 atagtattca gaaacatagt caattacatc gtcaggtctc tttaatttct ttcttgcatt
     8761 ggctttttct ttaaaaaatc ttttgcccag ttccagtgct gctttaatct gcaccgtctt
     8821 tgcaaaaccc accccttcaa ttttggataa ttcagatata ggcgctgaat ctattgccct
     8881 aagtgagccg aagtgattga gtaaaatctt tgcaatttcc tcagcactca taccttcttt
     8941 acctgttctt aagattatgg ctaggagttt ggataatggc aaattctctg cgcccagtcg
     9001 caccagcatc tctctcggtc tttcttcttc tatccactct tttataggac gacttaccct
     9061 gcgccctgcc acctcttttt taattctctc ccttaattcc tcacattctt ccagtgtttt
     9121 gatattcaat tgctgaataa tctcgtctct tttttcctga tctaacaatc ctcttcttga
     9181 gcattttaaa aaaacctttc catttttact gtaaaaagga taaatggcgt tcgaaatcat
     9241 aaaat
//
LOCUS       DAOTKU010000008         9217 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_7675_length_9217_cov_3.933202, whole genome shotgun sequence.
ACCESSION   DAOTKU010000008 DAOTKU010000000
VERSION     DAOTKU010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9217
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7675_length_9217_cov_3.933202"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(291..366)
                     /locus_tag="P5310_00390"
     tRNA            complement(291..366)
                     /locus_tag="P5310_00390"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(330..332),aa:Glu,seq:ttc)
     gene            complement(369..443)
                     /locus_tag="P5310_00395"
     tRNA            complement(369..443)
                     /locus_tag="P5310_00395"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(409..411),aa:Lys,seq:ttt)
     gene            complement(494..1510)
                     /locus_tag="P5310_00400"
     CDS             complement(494..1510)
                     /locus_tag="P5310_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020265003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase family protein"
                     /protein_id="HRU31856.1"
                     /translation="MGYLKTGKINTELLDRLISVYRGKEDPRVILGPSVGEDAAVISF
                     TDRYLIAKTDPITFVTENIGWYVVNVNANDILTRGATPKWFMSSILLPQGKATEEMLE
                     KISSQISEACEEIGVAVIGGHTEVTVGLDRPIIVGCMLGEVEKDRLIVTSNARAGDLI
                     VITKGIAVEGTAIIAREKQDELMLKGYSKSFISRCERFLYNPGISVYKEVSLCKEIDI
                     HSMHDPTEGGLRMGIYEVCRASRKGALIYESEIPVFEETKILCKEYGINPLGLITSGT
                     LIIALPKEESEKLIKLYEDNGILARVIGEIKPENYGIKMLSSSGQLLDLEVSEADEII
                     KIFS"
     gene            1563..2069
                     /locus_tag="P5310_00405"
     CDS             1563..2069
                     /locus_tag="P5310_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012308883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HRU31857.1"
                     /translation="MQKLVRTIAYIGIGSAIVCAGTMIIQIPIPQTRGYINLGDALVL
                     TIAMLFGSKIGGLAGGLGSALADILLGYAHWAPFTFAIKGIEGLIAGSLAYKAQNTIV
                     RVLSLILAGLEMVAGYFITETFMYGVGPAVVELPGNCFQAGGGVLISMLLFLAIKRVT
                     KGNKVWNI"
     gene            2080..3171
                     /locus_tag="P5310_00410"
     CDS             2080..3171
                     /locus_tag="P5310_00410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HRU31858.1"
                     /translation="MRIKPLISIAIILIIISSVSFTEASELTIRVLIYQGKEFSISGN
                     SLEVSIKNGEQLFIKNSDRVDGRVNSSRLSIKIIEGDKLNELSLDTSSTIDITGENIS
                     VNGNNYRGSLRVIYRSNSVIIVNILPLEEYLYGVVPSEVPYYWSKEALKAQAVLARTF
                     ALKNIRNTPGKDYDLDDSQNSQVYRGKSGEYNETNEAVDLTRGEVVLYNNSLASVYFH
                     STCGGHTELPENVWKVEKFPYLNGVECGYCNDSPWTNWEITVKRDAWEKTMGNGEIDI
                     EYDGSGRILRLNGIDGTRMRSVFNLPSTLIIGIEVEEKDVLIKGKGLGHGVGVCQWGM
                     KKMAELGFSYRDIISYYLPGVTVDAYRGF"
     gene            3152..4159
                     /gene="queA"
                     /locus_tag="P5310_00415"
     CDS             3152..4159
                     /gene="queA"
                     /locus_tag="P5310_00415"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HRU31859.1"
                     /translation="MRIEDFDLELPQELIAQEPVHPRDHCRLMVVYPDGKMEHRYFYN
                     LIEYLSPGDVLVLNNTRVRPYRLRGVKTTGANIEILLTRRLSDRVWEALANPSRRLHL
                     GTEVLFDKGLKCKTIQVREGGWRILEFNEEVESYLDELGEVPLPPYIHRKVKEEDYQT
                     IFARVNGSVAAPTAGIHFTDELLERIEAKGVEILYVTLHVGPGTFLPVRVEEIEKHYV
                     LPEEIEVTEETAERINRARSRGGKIVAVGTTVVRTLESVAEEDGRVKSYKGKTDLYIY
                     PGYKFKVIDRLVTNFHLPKSSLIILVSAFRDREIIMNAYREAIRLGYRFYSLGDAMLL
                     Y"
     gene            4162..5283
                     /gene="tgt"
                     /locus_tag="P5310_00420"
     CDS             4162..5283
                     /gene="tgt"
                     /locus_tag="P5310_00420"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HRU31860.1"
                     /translation="MGIRFQVLKEDSVTLARTGIIETPHGSFETPVFMPVGTQATVKT
                     VTPEEVVECGGRIILSNAFHLELRPGSKLIRDMGGLHRFMHWDRPILTDSGGYQIMSL
                     SPLVKINDTGIHFKSYLDGRELFFTPEEVIRIEENLGSDIMMPLDECIPYPSSKEKTR
                     SALDRTINWLLRSIKMKKRDDQALFGIIQGGMYNDLRMEAMERVASLDVDGIAIGGLS
                     VGEPKELMYSILECIMPYAPKDKPRYLMGVGTPLSLVEGVIRGIDMFDCIFPTRLARH
                     GGVFTNEGRFIVTNAEFTSDSRPIDESCSCYTCKNFSRAYIRHLFKAQEALGPRLTTI
                     HNLHYILHLMERIRESIRKDRIMELREEITYYYRKELSV"
     gene            5287..5562
                     /gene="yajC"
                     /locus_tag="P5310_00425"
     CDS             5287..5562
                     /gene="yajC"
                     /locus_tag="P5310_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012458882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HRU31861.1"
                     /translation="MGEGQSLLYPLIFWVVFFAIFYFILIRPQREREKKHREFINNLK
                     KGMKVITEGGFLGTIAEVKEKTVLIKFADNVVLEVLKDSVVAKQPKE"
     gene            5562..7748
                     /locus_tag="P5310_00430"
     CDS             5562..7748
                     /locus_tag="P5310_00430"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRU31862.1"
                     /translation="MESAIHGLVYLIFFLVIGILPGIFYPGTFDPFELPKLVFIRAGL
                     GLFFLLVLFYRRKSKQSNSQVLPYMIIFIIAMSLSTILSQYRWTSFHGEWERYMGLSS
                     MLPIVLAGLAPVFLDRKKIPLLKYAFLLGLVLVIIRSFRQILGYEIISIDSFGGRLLA
                     SLGNPDFLGEYLVMVIPLSMIEAIYSKKRVWKVITFVIFSLTFLALLLSGTRGSWLTF
                     LILLVIMPLIFVKPKTPRFVYRKTVFYVLFTGTLIAMLLYLPATVYIRLLLSILLILW
                     LYLYIRYLYPYMGEEIKARKMSIIIMAIIMIVLYFSLPYITSLIPHRGDVISETLKLR
                     LKAMTEVESGRWYMWKSAFRLIREEMISDPVRLLFGYGLDTTGKYLSHYKDIDSARMD
                     PMDNIVYADRAHNEYLEILLQTGLLGFLTFIILLITTVKKGIEIHRSTHPYRLFATAM
                     TLGVIGFIINGAVIFGTSVTYLYLFVMCGIIGVIYTPRGRLWRIKKDIIYRVIAVFAL
                     IFCIYTTYTGLRQLQSHKFLLKGVEAINRGELVSAEEYLDKSIEAYPIGYAFQRKLEI
                     YNIRLREEKSKEAFDKCIGLFPPMIEYVKYPTSVYYAMASIYMDGYELGLDKTMLDKV
                     IYYLKECIEYDKYHKPALRILARIYMDYLKDSESAYLYAKRYVEIEQRDIEMWRLLMR
                     TSKDVKDWETAWFSAKAIYLATEGKDREAERYLRESEEYISPSRNP"
     gene            complement(7726..9090)
                     /locus_tag="P5310_00435"
     CDS             complement(7726..9090)
                     /locus_tag="P5310_00435"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HRU31863.1"
                     /translation="MGPEFYLSILSGKIAIKATRLFHFGGTSFPGKLALSIHPSLLKE
                     LGERLKFKIFITGTNGKTTTARVISGILDRFGIDYVHNRAGANLPRGICTSLIDRFSL
                     RESLDNCIGIFEIDEAYLKILSNEIRPDVIIITNLFRDQLDRYGEVDTLARSFRSIAE
                     DNSETTFILNGDDPLVSSVAYNLSNKVLYFGLEYPGWENFSGIADIRSCPFCDANLVY
                     RKHYIGHQGDFECLNCGFKRVKPDIAAYNIDAGLDSSSFDISFKGLSYRVNVQLPGIY
                     NVYNVLSAILCCNILGLSLETSIRYLEEVKTAFGRGEKIKFKDKTIYILLAKNPAGFN
                     EIIQLLSGKKGIMVVLAINDNIQDGTDISWLWDVNFELLMGSSRYIIVSGRRAYDMGL
                     RLKYGGFDTSRLIIEEDVFDALSLAVDKTGDDTVYVIPTYTAMLELRRKLETLGITKG
                     FWKD"
BASE COUNT         2856 a         1734 c         1974 g         2653 t
ORIGIN      
        1 gtctgtatta ccggagttct tattaaagat agctattatc ttttctgtta cttctttctc
       61 tctagttgaa aaattaaaat tttttgcgcc tctgtttctg ttactgattc tgatactgtt
      121 tctgttactg tagagaccct ggtgggaggg tctatagagg gtctaaataa tttacctata
      181 ggtggatcaa ataaaaatat ctttcttaaa gatgttttta atattattaa gaagttatat
      241 ttgaagagaa tggaaactca aaacttgtaa ttttttgaat caataatcac tggtgccccc
      301 aagggaactc gaatccctgt ctcggccttg aaagggccgt gtcctaggcc tctagacgat
      361 gggggcactg gtgagccgca cagggattga acctgtgaca ccctgattaa aagtcaggtg
      421 ctctaccact gagctagcgg cccaacaagt cttattttaa cagataaatt ctgttttgta
      481 aatagtctaa aattcaacta aagatcttga ttatctcatc agcctcgctg acttcaagat
      541 ccaggagctg cccactagat gatagcatct ttattccata attctctggc tttatctccc
      601 caataaccct ggctaaaata ccattatctt cgtatagttt aatgagtttt tccgactctt
      661 ctttcggcag agctataatc aatgtcccgg atgttataag acccagagga tttataccgt
      721 actctttgca tagaattttc gtctcttcaa aaacaggtat ctctgattca tatataagtg
      781 cccccttcct ggaagccctg cagacctcat atatccccat tcttagacca ccctctgtcg
      841 gatcatgcat ggagtgtata tcaatctcct tacatagaga gacctcttta tatacactta
      901 tccctggatt ataaagaaac ctttcacatc tacttataaa acttttgctg taacctttaa
      961 gcataagttc atcctgcttt tctcttgcta taatggcagt cccttcaaca gcaatccctt
     1021 ttgttataac tataaggtcc cctgccctgg cattagaggt cactataagt ctatcctttt
     1081 ccacctcccc cagcatacat ccaactataa taggtctatc cagccctaca gtaacttccg
     1141 tatgacctcc tataaccgcc acaccaatct cctcacaggc ttcggatatc tgtgatgata
     1201 tcttttccaa catctcttct gtagctttcc cctgtggaag aagtatcgaa gacataaacc
     1261 attttggggt agcacctctg gtcaatatgt catttgcatt aacattaaca acataccagc
     1321 caatattctc tgtaacaaag gtaatcggat ctgtctttgc tataagatat ctatcggtaa
     1381 aagatataac cgctgcatcc tcaccaaccg aaggaccaag aattactctt gggtcctcct
     1441 tccctctata aacagatatt aatctatcaa gaagctctgt atttatcttt cctgtcttta
     1501 agtatcccat aaggtatatt ataatatata aaaagataaa aagaacaaca aggaggctat
     1561 agatgcagaa gttagttaga acgattgcat atataggtat aggatcagcg attgtatgtg
     1621 ctgggaccat gattatccag atcccaatcc ctcagaccag ggggtatata aatcttggcg
     1681 atgccctggt acttaccata gccatgctgt ttggatccaa gataggtggg ttagcaggag
     1741 ggcttggttc tgctctggct gatatattat tgggatatgc acactgggct ccttttactt
     1801 ttgctataaa aggtatagaa ggactgatag ctggaagttt ggcatataaa gctcagaata
     1861 cgattgtaag agttctttcc cttattctgg caggattaga gatggtcgct ggatacttca
     1921 taacggagac atttatgtac ggggtaggac cagcggtggt agaattgcct ggtaattgtt
     1981 ttcaggctgg cggtggagtg ctgatatcaa tgcttctttt tctggcaata aaaagggtaa
     2041 caaaagggaa taaggtctgg aatatataat ttaatgccga tgagaataaa acccctgata
     2101 tctattgcta taattctaat tataatttct tcagtatctt ttactgaagc ctcggagtta
     2161 actataaggg tccttatcta ccagggtaag gagttcagta tatctggaaa ctctctggag
     2221 gtcagtataa aaaacggaga gcagttattt ataaaaaact cagacagggt agacggaaga
     2281 gtgaattctt ccagattaag tataaaaatt atagaaggag ataaattgaa tgagctcagc
     2341 ctggatacaa gttcaactat cgatataacg ggagagaaca tttcggtaaa cggaaacaac
     2401 tacagaggta gcttaagagt tatatacagg agtaacagtg taatcatagt aaacatactc
     2461 cctctggaag aatatctata tggtgtagtt ccatcagaag tcccttacta ctggtcaaaa
     2521 gaagcattga aggctcaagc tgtccttgca agaacatttg cgctgaaaaa tataaggaat
     2581 actccgggga aagactatga cctagacgat tctcaaaact ctcaggtgta tagaggaaaa
     2641 tcaggagagt ataatgaaac caacgaggcg gtcgacctta caaggggaga agtagttctt
     2701 tacaataatt ctctcgccag tgtatacttc cattctacat gtggagggca tacagaactg
     2761 ccagaaaacg tatggaaagt tgagaaattt ccttacctga atggagtaga atgtggatac
     2821 tgtaacgact ctccctggac taattgggaa attactgtga agagggatgc atgggaaaag
     2881 accatgggaa atggagagat cgatatagaa tatgatggca gtggaaggat tttaaggctg
     2941 aacggaatag atggaaccag gatgagaagt gtattcaatc ttcccagtac acttattatc
     3001 ggaatagaag tggaagaaaa agatgtatta ataaagggga aagggctggg acatggtgtg
     3061 ggtgtctgtc agtgggggat gaaaaagatg gcagaactgg ggttttctta tagagatata
     3121 atctcatact accttccagg agtgactgta gatgcgtata gaggattttg atcttgaact
     3181 tccccaagaa ttgatagccc aggaaccagt gcatccacgg gaccactgca gactgatggt
     3241 tgtctatcct gatgggaaaa tggagcatag atatttttat aatctgatcg aatatctctc
     3301 cccaggggat gtactggtcc ttaataatac cagggtaaga ccatacaggt tgagaggagt
     3361 taaaaccaca ggagcaaaca tagagattct ccttactagg agattatcag atagagtctg
     3421 ggaagccctt gcaaacccgt caaggagatt acacttaggg acagaggttc tgtttgataa
     3481 aggattaaag tgtaaaacta ttcaggtaag agaaggggga tggaggattt tggaatttaa
     3541 tgaagaggta gaatcatatc ttgatgaact tggtgaggtc cccctgccgc catacatcca
     3601 cagaaaggtc aaagaggagg actatcaaac gatattcgcc agagtaaacg gctccgtagc
     3661 agctccaacc gctgggattc atttcaccga tgagcttcta gaaaggatag aagcaaaagg
     3721 tgtggagata ctgtatgtaa cactccatgt aggaccaggg actttcttac cggtaagggt
     3781 agaggagata gaaaaacact atgttcttcc cgaagagata gaagtaactg aagagacggc
     3841 agaaagaata aacagggcaa gatcaagggg tgggaagata gtagcggttg ggacaacggt
     3901 agtaaggaca ctggaaagtg tagctgaaga agacggtaga gtaaagagtt ataagggaaa
     3961 gacagacttg tacatatatc cgggatataa gttcaaggtt atagacagac tggttaccaa
     4021 ctttcatctc ccaaagtctt ctctgataat cctggtatct gcatttagag atagagagat
     4081 aataatgaat gcctatcgag aggcgataag attgggatac agattttaca gtttgggaga
     4141 tgctatgtta ttatattagc tatggggatt agatttcagg tattaaaaga ggatagtgtt
     4201 acattagcca ggacaggaat aatagagacc cctcatggtt catttgagac gcctgtattt
     4261 atgcctgttg gaacccaggc gacggtaaaa actgttacac ctgaagaggt agttgagtgt
     4321 ggggggagga taatattatc caatgcattc catctggagt taagacctgg tagcaaactt
     4381 ataagagaca tgggtggatt acacaggttt atgcactggg atagacctat cttgacagat
     4441 agcggtggat accagataat gagtctgagc ccgcttgtga agataaatga tactggcata
     4501 cattttaagt cttatctcga tggacgtgaa ctttttttta cacctgaaga ggtgataagg
     4561 attgaggaga acctcggttc agatataatg atgcctctgg acgaatgtat tccataccca
     4621 agcagtaaag agaagacaag atctgccctg gatagaacca tcaactggct tttaaggtct
     4681 ataaagatga agaagagaga tgaccaggca ctatttggta taatacaggg gggaatgtac
     4741 aatgacctga ggatggaagc gatggagcgg gtagcctctt tagatgtaga cgggatagct
     4801 ataggggggt taagcgtagg agagcctaaa gagcttatgt actccatatt agagtgtata
     4861 atgccttatg cgcccaagga caaaccaaga tatcttatgg gtgtggggac tcctctatca
     4921 ctggtagagg gggtgataag aggtatcgat atgtttgact gtatattccc aaccagactg
     4981 gcccgtcatg ggggagtatt tacaaatgag ggtagattta tagtcaccaa tgcagagttt
     5041 accagtgatt ctagaccaat agatgaaagc tgcagctgct atacctgtaa aaatttttcc
     5101 agagcatata taaggcatct attcaaggct caagaagcac tgggtccaag gcttacaact
     5161 atccataatc ttcattatat actacacctt atggagagaa taagagaatc gataagaaag
     5221 gaccggataa tggaacttag agaagagatc acttactatt atagaaagga gttgagtgta
     5281 taaacaatgg gagaaggaca atctttattg tatcccctta tcttctgggt tgtcttcttt
     5341 gcaatcttct actttatact tataagacct cagagggaga gggaaaagaa acacagagaa
     5401 tttataaaca atctgaaaaa gggaatgaag gtgataactg aaggaggatt tctgggaact
     5461 atagctgagg tcaaggaaaa aactgtcctc atcaaatttg ccgataatgt ggtactcgag
     5521 gtacttaaag actcggttgt tgcgaaacag ccaaaggaat aatggaatca gccatccatg
     5581 gtctggttta tctgatattc ttcttagtta taggtatcct gccagggata ttctacccgg
     5641 ggacatttga tccgttcgaa ctgcctaagc ttgtatttat aagggcaggc ttaggcttat
     5701 tttttttatt ggtcctgttc tacaggagaa agtctaagca gagtaactcg caagtattac
     5761 catatatgat catctttata atagcaatgt ctctctccac tatcctctcc cagtatcgat
     5821 ggacaagttt tcacggtgaa tgggagagat atatgggatt atcttccatg ctacctatag
     5881 tcctggctgg acttgccccg gtatttcttg accggaaaaa aattccactg ttaaagtatg
     5941 cgtttcttct tggattggta ctggtaataa taagaagttt cagacagata cttggatatg
     6001 aaattatttc catagatagt tttggaggta gacttcttgc ttcactgggg aatcctgatt
     6061 ttctgggtga gtatctggtg atggtgatac ctctatctat gatagaggct atatattcaa
     6121 agaaaagggt atggaaggtc atcacctttg ttatattttc cctgacattt ctggctctcc
     6181 tcttatccgg cacaagaggg tcctggctta catttcttat actacttgta attatgcctc
     6241 tcatatttgt aaaacccaaa acgcctcgtt ttgtctatcg taaaacagtg ttctacgtcc
     6301 ttttcactgg cacacttata gctatgctct tatatcttcc ggcaaccgta tatatcaggt
     6361 tattactatc tatcctgctt atcctctggc tatacctgta tataagatat ctctacccat
     6421 atatgggaga ggagataaag gctcgtaaaa tgtccatcat tattatggct attattatga
     6481 ttgtcctata cttctctcta ccttatataa ccagcctgat cccccataga ggagatgtga
     6541 taagtgaaac gttgaaactc agattaaaag ctatgacaga ggtggagagt ggaagatggt
     6601 atatgtggaa gtctgcattt aggcttataa gggaggagat gatctccgat ccggtaagac
     6661 tcctattcgg gtatggactg gatactacag gtaaatacct atcacattat aaagatatag
     6721 actcggcaag gatggatccg atggataata tagtttatgc cgatagggct cataatgagt
     6781 atctagagat actcttacag accggattac ttggcttttt aacttttatc attcttctta
     6841 taaccactgt caagaaagga atagagatac acaggagtac ccatccatac aggttatttg
     6901 caacagctat gacacttgga gtgataggat ttattataaa tggagctgtc atctttggca
     6961 caagtgttac ctatctgtat ctgttcgtga tgtgtggcat tatcggagta atatacacac
     7021 caagggggag attatggagg ataaagaaag atattattta cagagttata gcggtattcg
     7081 ccctgatctt ctgtatatat accacttata ccggattgag acaacttcag tcccataaat
     7141 ttctcttaaa aggggtagag gctataaaca gaggagaact ggtaagtgca gaagaatatc
     7201 tcgataaatc aatagaggct tatccgatag gttacgcctt tcaacggaag cttgagatct
     7261 ataatataag actgagagaa gaaaaatcta aagaggcatt cgataaatgt attggattat
     7321 ttccccctat gattgaatat gtaaaatatc caaccagtgt atattatgcg atggcatcta
     7381 tatatatgga cggatatgaa ctgggtttag acaaaactat gctggataaa gtaatttact
     7441 atcttaaaga atgtatcgag tatgataagt atcacaagcc tgcactgaga attctagcca
     7501 ggatatatat ggattatctt aaagattcag aatcagccta tctctacgct aagaggtatg
     7561 tagagataga acagagggat atagagatgt ggagactttt aatgaggact tcaaaagatg
     7621 taaaagactg ggagacagcc tggttctcag ctaaagccat atacctggca acagagggaa
     7681 aagacagaga ggctgaacgt tacctcagag agtctgagga atatatcagt ccttccagaa
     7741 acccttagtt atgccaaggg tttcaagttt tcgtctcagt tcaagcatag cggtgtatgt
     7801 cggtattaca taaactgtat catctccagt tttatcaacc gccagggaaa gggcatcaaa
     7861 aacatcctct tctatgataa gtctggaagt atcaaacccc ccatacttta acctcaatcc
     7921 catatcataa gctctccttc cggagactat tatgtatctt gagctaccca tcaagagttc
     7981 aaaatttaca tcccacagcc aggatatatc agtaccatcc tgtatattgt cgtttatagc
     8041 aagaacaacc attataccct ttttcccaga aagtagctgt attatctcat taaatccagc
     8101 tggattttta gccaggagta tatagatggt cttatcttta aatttgatct tttcacccct
     8161 cccaaaggcg gttttaacct cttccaggta tctgatagag gtctcaagag aaagtccaag
     8221 tatattgcaa cagagaatgg cagagaggac attataaacg ttatagatac ctggaagctg
     8281 aacattcacc ctgtagctta atcccttgaa agagatatca aaagaagaac tgtccaaccc
     8341 tgcatctata ttgtaagccg ctatgtcagg ttttactctc ttaaaaccac aattgagaca
     8401 ttcaaagtct ccctgatgac ctatatagtg ctttctatat accagattgg catcgcagaa
     8461 aggacaggac cttatatcgg caattccaga aaaattttcc catcctggat attccagccc
     8521 aaagtacagg actttatttg atagattata agcaacgctt gaaacaagag ggtcatctcc
     8581 gttcagtata aatgttgtct cactgttatc ctcagcgata ctcctgaaag atcttgccag
     8641 ggtatcaacc tcaccatatc tatccagctg gtcacggaag agattcgtaa taataataac
     8701 atcaggtctg atttcatttg acagtatctt cagataggct tcgtctatct caaatatacc
     8761 aatacagttg tctaaacttt ctcgcaaaga aaatctatca atcagactgg tacatatccc
     8821 tctggggaga tttgcaccgg ctctattatg cacataatct atcccaaatc tatccagtat
     8881 accggatata accctggcgg tggtggtctt tccatttgtc ccggtgataa atatcttgaa
     8941 ctttaatctc tccccaagtt cctttagaag agagggatgt atggaaagag ccagcttccc
     9001 agggaaactg gtccctccaa aatggaaaag ccttgtcgcc tttatagcaa tcttaccaga
     9061 caggatactg agataaaatt ctggacccat tatcttctac caagggtctt atataacttg
     9121 tcctcctctt tacatacagg atggctcaat ctcaacttcc agagcatatt gaaagatgga
     9181 agccccataa cctgctctaa cccccattct tttgcga
//
LOCUS       DAOTKU010000009         9086 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_7777_length_9086_cov_3.070092, whole genome shotgun sequence.
ACCESSION   DAOTKU010000009 DAOTKU010000000
VERSION     DAOTKU010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9086
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7777_length_9086_cov_3.070092"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="P5310_00440"
     CDS             complement(<1..440)
                     /locus_tag="P5310_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HRU31864.1"
                     /translation="MTESAAARFFNIFRIPELRNKVLFTLLMIGIYRIGIFIPVSGVN
                     MKSLEALMGQGTLLNMFDLFAGGGLSSFSIFAMGVIPYINASIIIQLLTVVIPALEKL
                     AKEEGEAGRRKLTQYTKYLSVVLAFVEAFGMSTWLRNLGVLEGIG"
     gene            complement(440..889)
                     /gene="rplO"
                     /locus_tag="P5310_00445"
     CDS             complement(440..889)
                     /gene="rplO"
                     /locus_tag="P5310_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HRU31865.1"
                     /translation="MVKLSELSPPDGAVKDRIRIGRGHGSGKGKTSGRGIKGQGSRSG
                     DGVMPGFEGGQTPLQRRLPHLPGFKNRFKKVYAIVNVGTLNQLEEGTVVTPELLLERG
                     IIKDLYDGLKILGGGSLEKSLTVRAHKFSGQAKEKITSSGGNVEVIS"
     gene            complement(882..1073)
                     /gene="rpmD"
                     /locus_tag="P5310_00450"
     CDS             complement(882..1073)
                     /gene="rpmD"
                     /locus_tag="P5310_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HRU31866.1"
                     /translation="MRMRIRITLKKSPIGYPEDQKATLRALKLNKLNATREIDATPCI
                     IGMINKVRHLLEIKEVNNG"
     gene            complement(1074..1583)
                     /gene="rpsE"
                     /locus_tag="P5310_00455"
     CDS             complement(1074..1583)
                     /gene="rpsE"
                     /locus_tag="P5310_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013290166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HRU31867.1"
                     /translation="MEENILDVQEAFELTERVISIRRVAKVVAGGKRFSFSALVAVGD
                     MQGHVGIALGKAGEAPDAIRKAINKAKKNMITVPMRGTTIPHEIVVDFGASKLVLRPA
                     SEGTGVIAGGTVRAILELAGIKNILTKSLGSTNPVNLAKVTVKALSMLRTPSEVASIR
                     GKEIKEILR"
     gene            complement(1586..1945)
                     /gene="rplR"
                     /locus_tag="P5310_00460"
     CDS             complement(1586..1945)
                     /gene="rplR"
                     /locus_tag="P5310_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HRU31868.1"
                     /translation="MSDRRLRREIRHKRIRRDVFGTPERLRLSVYRSLKHIYAQIIDD
                     TEGHTLVSASTVDPEISSQIKGITKTEEARIIGKVIAERAVAKGITRVVFDRGGYKYH
                     GRVKALAEGAREGGLQF"
     gene            complement(1958..2506)
                     /gene="rplF"
                     /locus_tag="P5310_00465"
     CDS             complement(1958..2506)
                     /gene="rplF"
                     /locus_tag="P5310_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002847908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HRU31869.1"
                     /translation="MSRIGRKPIPIPSGVDVEIKENSISVKGPLGTLEKPLPSGIIIK
                     KSDSTITVERPNDDKYYKALHGTVRTIVNNMIEGVTKGFSKTLEIIGTGYRAQKQGDR
                     LVINIGYSNPAIFEPDEVHFEVPDPTHVVVKGIDKERVGAVSANIRGCRPVNPYSGKG
                     IRYAGEAVRRKLGKAGKVGAKK"
     gene            complement(2518..2916)
                     /gene="rpsH"
                     /locus_tag="P5310_00470"
     CDS             complement(2518..2916)
                     /gene="rpsH"
                     /locus_tag="P5310_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HRU31870.1"
                     /translation="MATDPIADMLTKIRNGSKTRLESVDISPVSKLKLEILRILKEEG
                     YILDYKEIETEKFPFIRVYLKYVDNRTPAIAGMRRVSRPGLKIYAKRNDLPRVLGGLG
                     IAIVSTSSGIMTDRKARREGLGGEILCFVW"
     gene            complement(2929..3114)
                     /locus_tag="P5310_00475"
     CDS             complement(2929..3114)
                     /locus_tag="P5310_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011293056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HRU31871.1"
                     /translation="MARKVKVERSKLPPKYKVRAKNRCSRCGRPRGYIRDFGLCRICF
                     RELAHRGLIPGVTKASW"
     gene            complement(3132..3671)
                     /gene="rplE"
                     /locus_tag="P5310_00480"
     CDS             complement(3132..3671)
                     /gene="rplE"
                     /locus_tag="P5310_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HRU31872.1"
                     /translation="MPRLKDYYRETVVPYMMKRFNYENIMAVPKVEKVIVNMGVGDAI
                     QNPDLLKAAVSDLTIITGQKPVITKAKKSISNFKLRKGMSIGCMVTLRGNRMYEFLDK
                     LLNVSLFRIRDFRGVSPRSFDGRGSYTLGIKEQLIFPEIEYDKVVKVRGMDITIVTTA
                     KSDEEAYELLKALGMPFRS"
     gene            complement(3686..4009)
                     /gene="rplX"
                     /locus_tag="P5310_00485"
     CDS             complement(3686..4009)
                     /gene="rplX"
                     /locus_tag="P5310_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HRU31873.1"
                     /translation="MTLSHFKKGDTVEIIAGKDKSKRGKVLEVHPKEQILIVEGINRV
                     KKHMRPNPQLPTGGIILREAPLNWSKVMLVCPHCNKNTRIGIKVLSDRKVRVCKKCGE
                     IIDET"
     gene            complement(4009..4377)
                     /gene="rplN"
                     /locus_tag="P5310_00490"
     CDS             complement(4009..4377)
                     /gene="rplN"
                     /locus_tag="P5310_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003247583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HRU31874.1"
                     /translation="MIRPMSRLKVADNTGAKEILCIRVLGSGNQRYGYIGDIIVATVK
                     EATPNSSVKKSDIVKAVIVRTKNAIRRSDGTVVRFDENAAVLIDDQKNPRGTRIFGPV
                     ARELRDKDFMRIISLAPEVV"
     gene            complement(4374..4631)
                     /gene="rpsQ"
                     /locus_tag="P5310_00495"
     CDS             complement(4374..4631)
                     /gene="rpsQ"
                     /locus_tag="P5310_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019475474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HRU31875.1"
                     /translation="MARNERIGTVVSNKMNKTVVVAVESRYSHPLYKKVVKTVKKYKA
                     HDEENKCQVGDIVIIKETRPLSKEKRWMVESIIGKEGRGRV"
     gene            complement(4640..4837)
                     /gene="rpmC"
                     /locus_tag="P5310_00500"
     CDS             complement(4640..4837)
                     /gene="rpmC"
                     /locus_tag="P5310_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010285065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HRU31876.1"
                     /translation="MKPSVIRDLTDVELENRLKELKEELFNLRFQQSVGQLKNVSRIK
                     EVKKDIARVLTIMHLRQLGSK"
     gene            complement(4834..5268)
                     /gene="rplP"
                     /locus_tag="P5310_00505"
     CDS             complement(4834..5268)
                     /gene="rplP"
                     /locus_tag="P5310_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016148790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HRU31877.1"
                     /translation="MALIPTRVKYRKQHRGRMKGIATDGNYVAFGEYGLQALEPGWIT
                     SEELEAARIVLNRTLKRGGKIWIRVVADKPVTSKPAETRMGGGKGAPEHWVAVVKPGK
                     ILFEIAGLPKDIALEAFKLASCKLPIKTQMIYRNRLLGGEKL"
     gene            complement(5249..5950)
                     /gene="rpsC"
                     /locus_tag="P5310_00510"
     CDS             complement(5249..5950)
                     /gene="rpsC"
                     /locus_tag="P5310_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015043936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HRU31878.1"
                     /translation="MGHKVNPIGLRLGINKSWDAKWFANREYKDYLLEDIKLRGLVKD
                     KYHHAGIPRIEIERLPGRVRVIVHASRPGIIIGRGGVGIEELRKILADATGKNISITI
                     QEVKNPDLEAQLLAENVAFQIEKRVAYRRAMKHIVARALKAGAKGVKVSCNGRLAGAE
                     IARSEWYREGSVPLQRLRADVDYGFAEAMTTYGKIGIKVWLYRGDVLPEKKGTVEEEL
                     SLEEEGVTTDGADTD"
     gene            complement(5955..6290)
                     /gene="rplV"
                     /locus_tag="P5310_00515"
     CDS             complement(5955..6290)
                     /gene="rplV"
                     /locus_tag="P5310_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HRU31879.1"
                     /translation="MEAQAIARYVRISPRKANRVMALIRGKSIDEARAILKFSPGKAP
                     KFVEKVLNSAVANAVNNFELSEDDLYVARAYANKGPVLKRFRIGTQGRVNPVKKYTSH
                     LYIILEEKE"
     gene            complement(6300..6584)
                     /gene="rpsS"
                     /locus_tag="P5310_00520"
     CDS             complement(6300..6584)
                     /gene="rpsS"
                     /locus_tag="P5310_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HRU31880.1"
                     /translation="MGRSSKKGPYVHPKLLEKILEMNKNNEHRVIRTWSRSSMIIPEM
                     IGHTIAVYNGRKHVPIYITENMVGYRLGEFAPTRTFRGHGEVTRRSRELK"
     gene            complement(6584..7408)
                     /gene="rplB"
                     /locus_tag="P5310_00525"
     CDS             complement(6584..7408)
                     /gene="rplB"
                     /locus_tag="P5310_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HRU31881.1"
                     /translation="MGIKTFKPTSPGRRFYTTYDFSEVTKEEPESSLVVSIKKNAGRN
                     LHGRITIRHRGGGTKKKYRIIDFRRDKDNIPAKVVAIEYDPNRTARIALLFYADGEKR
                     YILAPLGLTVGATVMSGEQADVTPGNCMPIANIPIGTVVHNIELVPGKGGQIARAAGS
                     GAQIMAKEGEYALLRLPSGELRRINIRCRATIGQVGNLDHENIDLGKAGRVRWLGRRP
                     KVRGAAMNPVDHPHGGGEGKAPIGMPGPVTPWGKPTLGYKTRKRKNSDKFIVRRRK"
     gene            complement(7420..7707)
                     /gene="rplW"
                     /locus_tag="P5310_00530"
     CDS             complement(7420..7707)
                     /gene="rplW"
                     /locus_tag="P5310_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HRU31882.1"
                     /translation="MADSRDIIIRPVISEKTTLLRLSRKYEFVVSPFANKIEIKRAVE
                     DIFDVKVEKVNIVNKKGKEKRMGMYVGKTPAYKRALVTLAEGHSISFFEGL"
     gene            complement(7700..8323)
                     /gene="rplD"
                     /locus_tag="P5310_00535"
     CDS             complement(7700..8323)
                     /gene="rplD"
                     /locus_tag="P5310_00535"
                     /inference="COORDINATES: protein motif:HMM:NF012782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HRU31883.1"
                     /translation="MSKDVKIYNLLGEEVGTFQFEDIDSNRNILYYAVRVQLFNMIPK
                     TASTRTRGMVKGGGRKPWRQKGTGRARHGSIRSPIWKGGGVVFGPHPRTVRLSLPKKV
                     RRAALISALIDKIDSNQLILVESLDIDEPKTKNAVEVLNKLGIFSSCLVILDDSNTNF
                     ILATRNIPYIDIRTVSDVSVYDLLRYNYVLTTTSILEGLRERVISVG"
     gene            complement(8324..8974)
                     /gene="rplC"
                     /locus_tag="P5310_00540"
     CDS             complement(8324..8974)
                     /gene="rplC"
                     /locus_tag="P5310_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HRU31884.1"
                     /translation="MVIKGILARKVGMTQIFDDQGNRIPVTVVRTEGCKILQKKIVER
                     DGYNALLLGFEETRENRLNKPLRGFFKKLNAPFYRVLREIRVDEITDDFKVGDSITVN
                     TFKPGDRVDVTGTSKGKGFAGGVKRWHYGGGGAAHGSMFHRAPGSIGASSYPSRVFKG
                     QRLPGRMGGETVTVQSLEVVKVYEDEGLMLIKGAVPGSRNSLLIIKDAKKSKKESG"
     gene            complement(8987..>9086)
                     /locus_tag="P5310_00545"
     CDS             complement(8987..>9086)
                     /locus_tag="P5310_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006539131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HRU31885.1"
                     /translation="KRLIDILDPTPRTINALMKLNLPSGVDIEIKL"
BASE COUNT         2315 a         2398 c         1337 g         3036 t
ORIGIN      
        1 ccaatcccct caagaactcc aaggttcctc agccatgtgc tcatcccaaa agcttccaca
       61 aaggcaagaa ctacactgag atacttggta tactgggtaa gcttacgcct cccagcttct
      121 ccctcttcct ttgccaactt ttctaaggca ggtattacta ccgttaaaag ctggataatt
      181 attgaagcat ttatgtacgg aataactccc atagcaaata tagaaaaact gctgagccca
      241 cctccagcaa acaggtcaaa catattcaac agggtcccct gtcccataag agcctctaaa
      301 ctcttcatat ttactccgga gacagggatg aatatcccta tcctgtagat acctatcata
      361 agaagtgtga acaatacctt gtttcttaat tctggtattc tgaatatatt aaaaaatcta
      421 gcagcagcag attccgtcat caggaaatta cctccacatt ccccccacta gaggttatct
      481 tttcctttgc ctgcccggaa aacttatgag cccttacagt caaagatttc tccagactac
      541 cccctcccag aatcttaagc ccatcataca ggtctttaat tatccctctt tccaagagga
      601 gttctggggt tacaactgtt ccttcttcaa gctgatttaa agtcccaacg tttactatcg
      661 catatacctt cttaaatcta ttcttaaagc ctggtaaatg gggaagtctc ctttgaagtg
      721 gggtctgccc accttcaaaa cctggcataa ctccatctcc agaacgagaa ccctgccctt
      781 ttatacctct acctgaagtc ttaccttttc cagacccatg ccctctaccg attcttatcc
      841 tatcttttac tgcaccatca ggaggagata gttccgaaag tttaaccatt gtttacctcc
      901 ttgatctcta ggagatgtct taccttatta atcataccaa tgatacatgg tgtagcatca
      961 atttcccttg tagcatttaa tttgttcaac tttaaagctc ttaaagttgc cttttgatct
     1021 tcgggatatc ctattggact cttcttaaga gtaatcctta tcctcattct tattcacctc
     1081 agtatctctt ttatctcttt tcctcttatg ctagccacct cagatggtgt cctgagcatt
     1141 gaaagagctt tcacagtaac tttagccaga tttacagggt ttgtagaacc cagcgacttg
     1201 gtaaggatat tttttatacc agccagctcc agtatagctc ttactgttcc tccagctata
     1261 actccggttc cctccgatgc tggtctcaga acaagctttg atgctccaaa atcaacaacg
     1321 atttcatgcg gtatggtcgt acctctcatt ggaacagtaa tcatattctt cttcgcttta
     1381 tttatagcct ttcttatagc atctggagct tctccagctt taccaagagc gatgcccaca
     1441 tgtccctgca tatctccaac agctaccagg gcgctaaaac tgaatctctt cccccccgcc
     1501 acaaccttgg cgactcttct tatcgatatt actctttcag taagctcaaa ggcttcctga
     1561 acatctaaga tattttcttc catctttaaa actgtagacc tccctctctg gctccttctg
     1621 ccagggcttt tactctgcca tgatatttat atccacccct gtcaaatacc accctggtta
     1681 taccttttgc tacagctctt tcggcaataa ccttacctat aattctggct tcttcggtct
     1741 tcgttatccc cttgatttga gagcttatct ccggatcgac agtagaagcc gatacaaggg
     1801 tgtgtccttc agtatcatct ataatctggg catatatatg cttcagactc ctgtatacac
     1861 tcaatctcag tctttctgga gtcccaaaga catcccttct tatacgttta tgtcttattt
     1921 ctctacgaag tcttctatcg gacattgcat ttcctcctta cttcttggcg cctacttttc
     1981 cagccttacc tagctttctt cttaccgctt cacctgcgta tctgatgccc ttaccactat
     2041 agggattgac cggtctgcaa ccccttatat tggcagatac tgcccctacc ctttccttat
     2101 ctatgccctt tacaactacg tgggtaggat ctgggacttc aaaatgaact tcgtctggtt
     2161 caaatatcgc agggttacta taaccaatat tgataaccaa tctatctccc tgtttttgtg
     2221 ctctgtatcc agttcctata atttctaaag tcttggaaaa tccctttgta actccttcaa
     2281 tcatattatt aactatggtt cttaccgtac catgaagagc tttatagtat ttatcatcat
     2341 tgggacgttc aacagtaatg gtactatcgg attttttaat aataatcccg gaaggtaagg
     2401 gtttttcaag agttcctaaa ggacccttca cagatatact gttctctttt atctctacat
     2461 ctaccccaga tgggataggg ataggttttc tgccaatcct agacatatta tctcctccta
     2521 ccaaacaaag cagagaatct ctccgccaag tccttccctg cgtgcctttc tatcagtcat
     2581 aataccactg gatgtggaga ctatagctat acccaatccg cccagaactc tgggcaggtc
     2641 attccttttg gcatatatct tcaacccagg acgactcact cttctcatac cagctatcgc
     2701 aggtgtcctg ttatccacat atttgaggta taccctaata aatggaaatt tctctgtctc
     2761 tatctcttta tagtcaagta tatatccttc ttctttcagg attctcaaaa tctctaactt
     2821 taacttggag actggagata tatccaccga ctctagtcgc gtcttactgc cattccttat
     2881 ctttgttaac atatcagcaa tgggatcagt tgccatatct attacctcct accagctagc
     2941 cttagtaaca ccggggataa gcccacggtg agctaactct ctaaagcata ttctacacag
     3001 accaaagtcc ctaatatatc ctctgggtct gccacatctg ctacatctat ttttcgctct
     3061 tactttatat ttcggtggca atttacttct ttcaaccttt acttttctag ccatatactt
     3121 atcctcccaa attaacttct aaaaggcatt ccaagagcct taagaagttc ataagcctct
     3181 tcatcgctct tagctgtagt cacaatagtt atgtccatac ctcttacctt tacgacctta
     3241 tcatattcta tctctgggaa tattaactgt tcctttattc ctaaagtata acttcctcta
     3301 ccatcgaaag acctagggct tactcccctg aaatctctta ttctaaataa agaaacattg
     3361 agaagtttgt ccaaaaattc atacattcta ttgccccgta aagtaaccat acatccaata
     3421 ctcatccctt tacggagctt gaagttcgat atagactttt tagcctttgt gattacaggt
     3481 ttctgtcctg tgataatcgt caggtcactg actgcagcct tcagcaaatc agggttctgt
     3541 atagcatctc ctactcccat atttactatt actttttcaa ccttaggcac agccataatg
     3601 ttttcatagt taaatctctt catcatatat gggacaactg tttctctata atagtctttt
     3661 aatctgggca tatattccac ctctactaag tttcatctat tatctctcca cactttttac
     3721 atactctgac ctttctatca gatagaactt taatacctat cctcgtattt ttattacagt
     3781 gtggacaaac cagcataact ttactccagt ttaacggagc ctctctaagg ataatccctc
     3841 ctgttggaag ctgaggatta ggtctcatat gtttctttac cctgttaatt ccttccacta
     3901 taagtatctg ttctttcgga tggacctcta aaaccttacc tctcttagac ttatctttac
     3961 ctgcaataat ttctacagta tctccttttt taaagtgact cagcgtcatt ataccacctc
     4021 tggagctaaa gatattattc tcataaaatc tttatctctc aactctctgg caacaggacc
     4081 aaatattctg gtccctctgg gattcttctg gtcatctata agtacagcag cgttctcatc
     4141 aaatcttacc accgtaccat ctgatcttct tatcgcattt ttggtgcgga ctataaccgc
     4201 cttaacgata tcgctcttct tcacactgga gttaggtgta gcctccttta ctgtagctac
     4261 tattatatct cctatatacc catacctctg attaccgctg ccaaggacac ggatacacag
     4321 tatctctttt gctcctgtgt tatctgccac cttcagtcga gacattggtc gtatcatact
     4381 cttccccttc cttccttacc gataatactt tccaccatcc atcgcttttc tttactgaga
     4441 ggacgagttt cctttataat tacaatatca cctacctgac acttattctc ttcatcatga
     4501 gccttatact tcttcaccgt cttgacaact ttcttataga gaggatgaga gtatctgctc
     4561 tctaccgcta ccactactgt tttattcatc ttattgctta caaccgtacc gattcgttcg
     4621 tttctagcca taatacctcc tatttactcc ccaattgtct taagtgcata atagtaagaa
     4681 ctcttgctat atcttttttt acttccttta tcctggatac atttttgagc tgaccgacag
     4741 actgctgaaa tcttagatta aatagctctt ctttaagttc tttaagccta ttctccaact
     4801 ctacatctgt cagatctctt ataactgaag gtttcatagt ttctcaccac ctaaaagcct
     4861 attcctatat atcatctgag tctttattgg cagcttacaa ctggcaagtt taaaagcctc
     4921 cagagctata tcttttggta atcccgcgat ctcaaagaga atcttacccg gctttacaac
     4981 cgctacccaa tgttctgggg ctcctttacc gccacccatt cttgtttctg ctggttttga
     5041 cgtgaccggc ttatccgcga caacccttat ccatatctta ccgccacgct ttagtgtcct
     5101 atttaaaact attctcgctg cctccaactc ttcactggtt atccatccag gctctaaagc
     5161 ctgcagccca tattctccaa aagccacata attcccatcc gtagcgatac ccttcatcct
     5221 acccctgtgt tgctttctat actttactct agtcggtatc agcgccatct gtagtaacac
     5281 cttcctcttc cagtgatagt tcctcttcta ctgtcccctt cttctctggt aatacatcac
     5341 ctctgtatag ccagaccttt atacctatct taccataagt ggtcatagct tcagcaaatc
     5401 cgtaatcaac atcagctctt aatctctgaa gaggaacgct cccttctcta taccattcac
     5461 ttctggctat ttcagctcct gccaatctcc cattacagga tacctttact ccctttgccc
     5521 cagccttaag tgctctggca acaatatgct tcatagccct tctatatgcg accctttttt
     5581 ctatctgaaa tgccacattc tctgccaata attgcgcttc taaatcaggg ttctttactt
     5641 cctggatagt tatagatata tttttccctg tagcatcggc aagtatctta cgtaactcct
     5701 ctatacccac tcctcctcta cctattataa ttccgggtct ggaggcatga actattaccc
     5761 tcacccttcc tggtaatctt tcaatctcta ttctgggaat accagcatga tggtacttat
     5821 ctttgacaag tcctcttaat tttatatcct ctaataaata gtccttatat tccctgttag
     5881 caaaccactt agcatcccag gatttattta ttcctaatct taatccaatc ggatttactt
     5941 tatgccccat ctactcactc cttctcctca agaataatat ataagtgact ggtatatttc
     6001 tttacaggat tcactctacc ctgagtacct atcctgaatc ttttcaagac aggaccctta
     6061 ttggcgtacg ccctggccac atagaggtca tcttcagaaa gttcgaagtt atttactgca
     6121 ttagccaccg ctgaattgag aaccttttct acaaatttag gggcttttcc aggagaaaac
     6181 tttaatatag ctctggcttc atctatactt tttcctctga taagagccat aactctatta
     6241 gcctttctcg gagagatcct cacatatctt gctattgcct gcgcttccat aaaaacctcc
     6301 tacttcaact ctctagaacg tctggtaacc tctccgtgtc ccctaaacgt tctagtagga
     6361 gcaaattcac caagtctata accaaccata ttttccgtta tatatatagg gacatgtttt
     6421 ctaccgttat aaaccgcaat tgtatgacca atcatttcag ggataatcat agaacttctg
     6481 gaccaggttc ttatcaccct atgttcatta ttcttattca tctctagtat cttctctaga
     6541 agcttgggat gcacataagg tccctttttg ctagaacgac ccattacttc ctcctcctaa
     6601 caataaactt atcagaattc tttctttttc ttgtcttata accaagagta ggcttacccc
     6661 atggagtaac aggtccaggc attccaatag gagctttacc ttcaccacca ccatgaggat
     6721 ggtctaccgg attcatagca gcgcctctta cttttggtct tcttccaagc cagcgaactc
     6781 ttcctgcttt ccccagatct atattttcat ggtctaagtt ccctacctgt cctatagtgg
     6841 ctctgcatct aatattaatt cttctcaatt ctccagatgg gagtctcagg agggcatatt
     6901 caccttcttt agccattatc tgtgctccag atccagctgc cctggctatc tgaccacctt
     6961 taccaggaac aagttcaata ttatgaacca cggttcctat aggtatattg gcgatgggca
     7021 tgcagtttcc aggggtcaca tctgcctgct ccccggacat aacagtagca ccaaccgtta
     7081 aacctaaagg ggcaagtata tacctcttct caccatcggc ataaaagaga agagctattc
     7141 tggcagttct gtttggatca tactctatag ccacaacttt agcaggaata ttatctttat
     7201 ctcttctgaa atctattatt ctgtacttct tctttgtccc gccacctcta tgtctgatag
     7261 ttatacgtcc atgcaggtta cgtccagcat tcttctttat agatactacc agactggatt
     7321 ctggctcttc ctttgtaacc tcactgaaat catatgtggt ataaaatctt cgtccaggag
     7381 atgttggttt aaatgtcttt atccccataa tttcactcct taaagtcctt caaagaagga
     7441 tattgaatga ccttctgcca atgtaacaag cgctctttta tatgctgggg tcttaccgac
     7501 atacatcccc attctctttt ctttcccctt cttattaaca atattgacct tttccacctt
     7561 aacatcaaat atgtcttcta cagccctttt aatttcgatc ttattggcaa aaggagatac
     7621 tacaaactcg tactttctgg atagcctcaa gagagtagtc ttttcactta taactggtct
     7681 tattattata tctctagaat cagccaactg agatcaccct ttctctaagt ccttcaagaa
     7741 tggatgtggt agtcagtacg tagttatatc ttaacagatc atatactgag acatcgctca
     7801 cagttcttat atcgatatac ggtatatttc tcgtcgccag aataaagttt gtattactgt
     7861 cgtcaagaat aaccagacag gacgaaaata taccgagctt attcaagact tccacagcat
     7921 tcttagtctt aggttcatca atatccaaac tttctaccag aatcagttga ttactatcta
     7981 tcttatctat aagagcagag ataagagcag ctctcctgac cttcttaggc agggacaacc
     8041 ttacagtcct tggatgggga ccaaaaacta cacccccacc cttccagata ggagacctta
     8101 tacttccatg tctagctcta cctgtacctt tctgcctcca gggttttctt cccccacctt
     8161 tcaccatacc tctggttctg gtcgatgcgg tcttggggat catattgaac agttgaactc
     8221 ttaccgcata gtagagaata ttcctgttcg aatctatatc ttcaaattga aatgtcccta
     8281 cctcttctcc tagcaagtta taaatcttaa catctttact catttacccc gactccttct
     8341 tactcttctt tgcgtctttt attatcaaaa ggctatttct ggacccggga actgctcctt
     8401 taataagcat cagtccctcg tcctcataga ccttcacaac ttccagagat tgtaccgtca
     8461 cagtctctcc tcccatacgt ccaggaaggc gttgcccctt aaatactctg gaaggataag
     8521 agctggcacc tatagaacct ggagctctgt ggaacataga gccatgagca gctcccccac
     8581 caccatagtg ccaccttttt actccaccag cgaaaccttt tcctttcgat gtccccgtta
     8641 catccactct atccccgggt ttgaatgtat taacggttat actatctcca actttaaagt
     8701 catcagttat ctcatccact cttatctctc tcagcactct atagaatggt gcattcaact
     8761 tcttgaagaa acccctgaga ggtttattta gtctattctc tctggtctct tcgaaaccta
     8821 aaagtaaggc attatacccg tctctttcta caatcttttt ctgaagaatc ttacaacctt
     8881 cagtccttac aactgtgaca gggattctat tcccctggtc gtcaaaaatc tgagtcattc
     8941 caacctttcg tgctagtata ccttttataa ccatagccat cacatcctat agttttatct
     9001 ctatatctac accagagggt agattcaact tcataagagc atttatcgtc cttggagtag
     9061 gatccagtat atctataagt ctctta
//
LOCUS       DAOTKU010000010         8108 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_8853_length_8108_cov_3.094126, whole genome shotgun sequence.
ACCESSION   DAOTKU010000010 DAOTKU010000000
VERSION     DAOTKU010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8108
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8853_length_8108_cov_3.094126"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1676
                     /locus_tag="P5310_00550"
     CDS             <1..1676
                     /locus_tag="P5310_00550"
                     /inference="COORDINATES: protein motif:HMM:NF025925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HRU31886.1"
                     /translation="CGQCLVKAKGHLSEISPVETDLISEDKLKEGYRLACQTYIKGDV
                     DVEIPSQTRTYAHRILTTGIKTEDLRINPAVKSYNINLSPPSLDDQRSDLDRIEEAIG
                     FKLEIPGINMLKDIPEILRENAFNIEVISYYKSLLEIRRPENKSIYGVAIDIGTTTVV
                     VYLLDLRKGEIDVISAMNPQAKRGADVISRIDYAAKGGLEELQTSIVEEINSLIDQLC
                     DRQGIENRDIYDVAVVGNTTMMHLFLGISPRYIAVSPYIPVFTKSLMVRASEIGIDIN
                     PNGICYILPNIAGYIGADTVGMILATGLYKEDGVRLALDIGTNGEMVLKDGDKIFSCS
                     TAAGPAFEGANITYGMRGSRGAIDHVNIDDGHIIVHVIDDVEPVGICGSGLLDAVAVM
                     LELGLLDETGRIIKPGGSLFESLVREKNNGLEFLLEAHNKKIPINQRDIRELQLAKGA
                     IRSGIEILLEEAGRTYSDIETIYLAGAFGTYLDPESAIKIGLLPPVPLNKVYSVGNAA
                     GEGAKLALIDVDFREIAEDISRKVRYIELSSRIDFQDKFMDSLYFEVVR"
     gene            1673..2782
                     /locus_tag="P5310_00555"
     CDS             1673..2782
                     /locus_tag="P5310_00555"
                     /inference="COORDINATES: protein motif:HMM:NF024785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HRU31887.1"
                     /translation="MRYLTPPPGFRDYLPEEVEKIRLIEKTIRETCESFGYREVITPT
                     LEYADIILLGTNIKDDTILYKVIDKDGALLSIRPEFTTSIARLITTRFRDKDGPFRFY
                     YKGKVFRNIPFQPLMQKEFSQGGAELIGEPSLSGDIEIIALIEEIFERLNLEVYIEAS
                     HAKIIPAIIDATQAPILESIKSGDFVSIRSHIRDKEILSFIEALPFLNGGKEVIDKAS
                     RYINSIPMLEEPLNQLSELISNLEELGYNKTKINLGVIRDFEYYTGITFEGYLEDLGL
                     PVLGGGRYDGLLEKFGLPLPATGFAIGVERVASRIKREESIPDLLIGYSKDKFKEAMR
                     IAKEERKKGLKVEIILSDREDELNIKKGRKKIFIC"
     gene            2776..3396
                     /gene="hisG"
                     /locus_tag="P5310_00560"
     CDS             2776..3396
                     /gene="hisG"
                     /locus_tag="P5310_00560"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007504542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HRU31888.1"
                     /translation="MLAIALPSGRLMEECVKLLENSGVSFPKEILESRRLSGIIDNKK
                     FLICKPKDVPIYVEYGTADIGMVGKDILLEENTQVYELYDLGIGRCRIVVAIPNSLEP
                     EEIFFRGDIIRVATKYPRIARDFFNKRGISIEILNLSGSIELAPVTGIADVIVDIVST
                     GRTLKENNLRIWEEIAYSSARLIANRASLHIKADEIEDLLNKLKRG"
     gene            complement(3393..5246)
                     /locus_tag="P5310_00565"
     CDS             complement(3393..5246)
                     /locus_tag="P5310_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013413006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRU31889.1"
                     /translation="MPDRREERPAVHNHGFRGGPGGPHSMFSKVKPEDPLGTLKRLWG
                     YIKQYRLRFFLIFIIVFVTALLSLINPYLIGRAIDQYIIPKDFKGLFSLIILMISIHI
                     ANSLFIWLQGYLNITTVQKIVLQLRKDLFGKLQSLPLRFFDSSQHGDLMSRLTNDIDT
                     VNMSLNTSITQVFSSIVTLTGTLLVMLRLSPMLTLISMATIPFTLLVTSFISRHTREY
                     FFSNQTILGKLNGLIEENISGLKVVKVFSRERKEIERFGNINRELREVGTKAQIYSGV
                     MGPLMNVLNNLSFALIAGFGGWFTVQNIITVGTIASFITYSRQFTRPINELANQFNML
                     QSAMASAERVFKVMDESPEPADLPDAVELKDVKGDVEFRNVCFSYKEGVPVLKNVTFH
                     AKPGQTIALVGPTGAGKTTMVNLLTRFYDVDSGSILIDGIDIRRIKRSCLRSLLGIVL
                     QDTYLFSDSVKENIRYGRLSATDEEVKMAARLAHAEHFILRLPHGYDTVLSDGGENLS
                     QGQRQLLAIARAILADPAILILDEATSNIDTRTEKYIQAAMLNLMEGRTSFVIAHRLS
                     TIRNADMILVINDGEIIERGTHRELLEKKGFYYSLYMSQFAAEVEGVTKTG"
     gene            complement(5239..6972)
                     /locus_tag="P5310_00570"
     CDS             complement(5239..6972)
                     /locus_tag="P5310_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRU31890.1"
                     /translation="MKRLLVYLKPYWKMVILAPLLMVIEVVCDLSQPTLLAKIVDDGI
                     AKGDIDLIIRTGLLMVGVAFIGMIGGVGCTIASSIASQGFGADLRSAVFRKIQSFSFS
                     NLDRFKPSTLITRLTNDITQLQQLVLMSLRIVVRAPLLFLGGILMAVSINSRLSTILF
                     IAIPLQIIAFLYLMKKGFPLFTIMQQKIDRVNAVIRENLAGIRVVKAFVREDMEKSRF
                     NSANEDLMNTSIRAFMPIITLMPFIMLVMNLSVVAVLWFGGLLVARENMHVGEIMAFI
                     NYLTQILFSLMMIGNIFIFISRASASAKRVSEVLDTEVDIKNPANPDTNPITEGEVIF
                     EDVSFSYDGTGEDPVLEDISFTAYPGETVAIIGTTGSGKSTLVSLIPRLYDVTKGRIL
                     IDGRDVREIDIDILRSAISIVLQETILFSGTIKENILWGNEFATDEEVIEIAKIAQAH
                     DFIMSFPDGYDTQIGQRGVDLSGGQKQRIAIARALIKKPVILILDDCTSAVDLATERR
                     ILRGLKSISRCTTFIIAQRISTVMNADKILVLDDGRLIAQGTHSELLVTCPIYQDIYK
                     SQIGEEAVIDA"
     gene            complement(6969..7463)
                     /locus_tag="P5310_00575"
     CDS             complement(6969..7463)
                     /locus_tag="P5310_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRU31891.1"
                     /translation="MVCKDNKDDTSLGSLLWQVIREHFIRHHHLLSKIGIYKGQPQIL
                     ELLWEKDGLTQKDIAESLKLRPSTVTVTLGRMEKAGLLRRETDPRDMRVSRVYLTEKG
                     RGLKKGIEEIDKMLEDECFAGFTFEEKILLRRFFIQIRDNLREVNRKEENLSSVEIIT
                     GGKK"
     gene            complement(7590..>8108)
                     /locus_tag="P5310_00580"
     CDS             complement(7590..>8108)
                     /locus_tag="P5310_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRU31892.1"
                     /translation="VSKAMDDRIGCAVLIEAIKRSDPYHTLYGTFSVQEEMGLVGASV
                     VAFNINPEMAIALDVTAWSDTPKGYKRMSIELGKGPCIKIKDSASISDRRVVDKLRNI
                     AEANNIPYQIEILIFGGTNAAALQRTSAGIVSGTLSIPCRYVHSPCEMIDIDDAENAV
                     KLLNALVEDSSI"
BASE COUNT         2576 a         1642 c         1567 g         2323 t
ORIGIN      
        1 ggtgtggaca gtgtctggtg aaagcaaagg ggcatctttc tgagatatct ccggttgaaa
       61 cagacctcat ttcagaggat aaactaaagg agggatacag actcgcctgt cagacatata
      121 taaagggtga cgttgatgta gagattccgt ctcagaccag aacatatgca catcgcattc
      181 ttactacagg cataaagacg gaagacctca ggataaaccc tgctgtaaaa agctataaca
      241 taaacttatc acctccatct ttagatgatc agagatcaga cctggataga atagaagaag
      301 ccattgggtt taagctggaa ataccaggga taaatatgct gaaagatata cctgaaattc
      361 tcagagagaa cgccttcaac atagaggtaa tttcctatta taaatctctt ttagagataa
      421 gaagaccaga aaataagtcc atctatgggg tggcaataga tataggaacc acaacagtgg
      481 tggtatatct tttagacctt agaaagggag agatagatgt aatttccgca atgaatccac
      541 aggctaaaag aggggcagat gtaatttcca ggatagacta tgccgctaaa ggaggactag
      601 aagaattaca gacctcaata gtagaagaga ttaactctct tatagatcaa ctatgtgata
      661 ggcaggggat agaaaacaga gacatatacg acgtagctgt tgttggtaat accactatga
      721 tgcacttatt tttgggcata tcacccaggt acatagctgt atcaccatat atccctgtgt
      781 ttacgaaatc tctgatggtc agagcctctg agatagggat tgatataaat cctaacggga
      841 tatgctatat acttcccaat atagctggat atattggagc agatactgtg gggatgatcc
      901 tggctactgg attatataag gaggatggag taagactggc attagatata gggaccaatg
      961 gggagatggt attaaaagat ggagataaaa ttttctcctg ttctaccgca gctggacccg
     1021 ccttcgaggg agctaatatc acttatggaa tgcgaggatc taggggagct atagaccatg
     1081 tgaacataga tgatgggcac ataattgtcc atgtaataga tgatgtggaa ccggtgggta
     1141 tatgcggttc aggtttatta gatgcggttg cagttatgct agagttaggt ctattagatg
     1201 agacaggaag gataataaaa ccaggtggaa gcttatttga aagtcttgta agagagaaga
     1261 ataatggtct tgagttttta ctggaggctc ataataaaaa gattccaata aatcagagag
     1321 atataagaga gctacagctg gctaaagggg ctatcagatc tggaatagaa atactcttag
     1381 aagaggcggg gagaacatac tctgacatag agaccatata tctggcaggc gcctttggga
     1441 catatctgga cccagaaagt gctattaaaa taggactcct cccaccagta cctttaaata
     1501 aggtatattc agtgggaaat gctgccggag aaggagcaaa acttgctctt atagacgtag
     1561 atttcaggga aatagcagag gatatctccc gaaaggttcg gtatatagaa ctttcttcga
     1621 ggattgactt ccaagataag tttatggatt ctctctattt tgaggtggtt agatgagata
     1681 tcttactcct cctccaggat tcagagatta tctccctgaa gaggttgaga agataagact
     1741 aatagaaaag acgataagag agacctgtga atcttttggg tatagagagg tgataactcc
     1801 aacattagag tatgcggata tcatcttact ggggaccaat ataaaagatg ataccatact
     1861 ttataaggtg atagataaag atggagcatt attatcaatt agacctgaat ttaccacctc
     1921 tatagcaagg ttaatcacaa ccagattcag agacaaagat ggacctttca ggttctacta
     1981 taaaggtaag gtcttcagaa atatcccctt ccagccttta atgcaaaagg aattcagcca
     2041 ggggggagca gaactgatag gagagccatc actatcggga gatatagaga taatagctct
     2101 tatagaggag atttttgaaa gactaaacct ggaggtctat atagaggcga gccatgccaa
     2161 aattattccc gccattatag atgccactca ggctccaata ctcgagtcca taaagagtgg
     2221 cgactttgta agtataaggt cccatatacg ggacaaagag atcctctcat ttatagaggc
     2281 tcttccattt ttaaacgggg ggaaagaagt tatagataag gcttctaggt atataaactc
     2341 catcccaatg ttagaagaac ctttaaacca gctatctgag cttatatcta acctggagga
     2401 actgggatat aacaagacaa agataaacct tggcgtaatc agagatttcg aatactatac
     2461 cggtataaca tttgaaggct acctggagga cctggggtta cccgttttgg gaggagggag
     2521 gtatgatggg ttactggaaa aatttggctt accgctacct gccacaggtt ttgctatagg
     2581 ggtagagaga gtagcttcca gaattaagcg ggaagaatcc atccctgacc ttctgatagg
     2641 ctatagtaaa gacaaattta aagaggcaat gagaatagca aaggaggaga ggaaaaaagg
     2701 tttaaaggta gagataattc tctctgacag agaggatgaa ttaaacataa aaaaaggaag
     2761 gaagaagata tttatatgtt agctatagca ttaccttctg gcagattaat ggaagaatgt
     2821 gttaagttat tagaaaattc aggagtttct ttcccgaaag agattttaga aagcagaaga
     2881 ctatcaggaa taattgataa taaaaaattt cttatctgta aacctaaaga tgtacctata
     2941 tatgtggaat atggaacagc tgatattggg atggtgggga aggatatact cttagaagaa
     3001 aacactcagg tatatgaact gtatgattta ggaattggaa ggtgccggat agttgttgcc
     3061 ataccaaata gcctggagcc tgaagaaata ttcttcagag gagatatcat cagagtagct
     3121 accaaatatc ccaggatagc aagagatttt tttaataaaa gggggatctc aatagaaatc
     3181 cttaatctca gtggttctat agaacttgca ccagttaccg gtatagcaga tgtcatagtc
     3241 gatattgttt ctacaggaag aacactgaag gaaaataacc tgaggatatg ggaggagata
     3301 gcctactcct ctgccagact tatagccaat agagccagct tacatataaa agcagatgag
     3361 atagaagatt tactgaataa gctaaagagg ggttagcctg tctttgtaac cccttctacc
     3421 tctgcagcaa actgactcat atagaggcta tagtagaaac ctttcttctc cagtagttct
     3481 ctgtgggtcc ctctctcaat aatttctcca tcatttatta ccagtatcat atccgcattg
     3541 cgtatggtac tgagacgatg agcaataaca aaactggtac gcccctccat aagatttaac
     3601 atggcagctt gtatatactt ctcagtcctt gtgtctatgt tactggtagc ctcatcaagt
     3661 ataagtatag ctggatcagc cagtatagct cttgctatgg caagtagctg tctctgcccc
     3721 tgacttaaat tctcaccacc atccgaaagg acagtatcat agccatgggg taatctcagg
     3781 ataaaatgtt ctgcatgggc aagtcgtgct gccatcttaa cttcctcatc ggtagcggat
     3841 aaacgtccat atcttatatt ctccttcaca gaatcggaaa aaagatacgt gtcttgaaga
     3901 actataccaa gtaaagaacg cagacagctt ctctttattc ttcgtatatc tatcccatct
     3961 atcaaaatag atccgctatc cacatcataa aatcgcgtca atagattaac cattgtggtc
     4021 ttaccggccc cagttggacc gaccagggct attgtttgcc cgggcttcgc atgaaaagtc
     4081 acattcttca atacaggaac cccttcttta taagaaaagc aaacattcct gaattcaaca
     4141 tcccctttaa catctttaag ctccacagca tctggcaaat cagcaggttc aggagattca
     4201 tccataactt taaataccct ctctgcactg gccatagcag attgcaacat attgaattga
     4261 ttcgccagct cattgatagg acgggtaaac tgtcttgaat atgttataaa actggctatt
     4321 gtcccaacag ttattatatt ctgaacagta aaccagccac caaaaccagc tataagggca
     4381 aaactgaggt tattaaggac attcatcaat ggacccatca caccggagta aatctgagct
     4441 ttagttccaa cttctctcaa ttctctattg atattgccga aacgttctat ctctttcctc
     4501 tctctggaaa aaacctttac aaccttaagc ccagagatat tttcttcgat aagtccatta
     4561 agttttccca aaatagtctg gttggagaag aagtactctc ttgtatggcg agagataaaa
     4621 ctggtaacca gaagagtaaa cgggatagtt gccatactta tcagagtaag cattggactt
     4681 aaccttaaca ttactaataa tgtacctgtc aaagtaacga tactggaaaa aacctgcgtt
     4741 atgctcgtat ttaaagacat atttacggta tctatatcat tcgttaacct gctcatcaaa
     4801 tctccatgct ggctgctatc aaaaaaccgc aggggcaaac tctgcagttt tccaaaaagg
     4861 tcttttctca actgaaggac tatcttctga actgtagtta tattaagata tccctgaagc
     4921 catataaata aagaattagc aatatggata gagatcataa ggattataag tgagaataaa
     4981 cctttaaaat ctttcggtat tatatactgg tctatcgctc tccctataag atagggattt
     5041 atcaacgata aaagagcagt tacaaatact atgataaata tcaagaaaaa tctaagcctg
     5101 tactgtttta tatatcccca taatctcttc aaggttccaa gtggatcctc tggctttact
     5161 ttggaaaaca tggagtgtgg acccccaggt cctcctctaa aaccatgatt atgtaccgca
     5221 ggtctctctt ctctacgatc aggcatcgat tacagcctcc tctcctatct gtgatttata
     5281 gatatcctga taaatagggc aagtcaccag aagttcagaa tgagtcccct gagctataag
     5341 tcttccgtca tcgagcacca gtatcttatc agcattcata acagtactta ttctctgagc
     5401 tattataaag gtagtacatc tacttatact ctttaatcct ctcaagattc tccgttcagt
     5461 agccaggtcg accgcactcg tacagtcgtc cagaatcagt ataactggct tttttataag
     5521 agccctggct atcgctattc tctgtttttg cccaccggat aaatctaccc cacgctgtcc
     5581 aatctgagta tcatatccgt cagggaagct cattataaaa tcatgagcct gcgctatctt
     5641 cgctatctct ataacctctt catctgtagc aaactcattc ccccagagaa tattctcttt
     5701 aatagtacct gagaatagta tagtttcctg gaggactata cttatggcgg acctcaatat
     5761 atcaatatca atctctctga catctcttcc atctatcagt atcctcccct tcgtaacgtc
     5821 atataatctg ggtataagac tgaccaaagt ggactttcct gaaccagtag tacctattat
     5881 agctactgtt tctccaggat aagcggtaaa agatatatct tcaaggacag ggtcttctcc
     5941 cgttccatca taactaaagg atacgtcttc aaatataacc tcaccttcag tgatggggtt
     6001 agtgtctgga ttcgccggat tttttatatc cacctcagta tccagcactt cactaactct
     6061 tttagcagag gcgctggctc tgcttataaa tataaatata ttccctatca tcatcagtga
     6121 aaatagaatt tgagtaagat aattgataaa agccattatt tcaccaacat gcatattctc
     6181 tctggccact aaaagaccgc caaaccacaa aacagcaaca acacttaaat tcatcaccag
     6241 cattataaat ggcataagag ttattatcgg cataaacgct cttatagagg tattcataag
     6301 gtcctcattg gcagagttaa atctactttt ttccatatcc tcacggacaa aagccttcac
     6361 cactcttatt ccagccagat tttctcttat aactgcgtta actctatcta tcttttgttg
     6421 cattatggta aacagtggaa atcccttctt cataaggtat aaaaatgcga ttatctgaag
     6481 tggtatagca ataaaaagaa tggtggataa tctactgtta atagatacag ccataagaat
     6541 tccacccaga aaaaggagag gagccctaac cacaattctc agagacatca atacgagctg
     6601 ttgaagttga gttatatcgt tggtgagacg agttataagg gttgaaggct taaatctatc
     6661 cagatttgag aatgaaaagg actgaatctt tctgaatact gcagaacgca ggtccgctcc
     6721 aaatccctgg ctggcaatac ttgatgctat agtacaacct actccaccga tcatacctat
     6781 aaatgccacc ccgaccatca acaagcctgt ccttataatc agatcaatgt ctccttttgc
     6841 aattccatca tcaacaatct ttgccagtaa tgtaggttgg gatagatcac atacaacctc
     6901 tataaccata agtaatggag ctaaaataac cattttccag tatggtttta gataaaccag
     6961 taacctcttc acttcttacc tccagttatt atttcgaccg atgacaaatt ttcttccttc
     7021 ctgttaacct ctctcagatt atcccttatc tggataaaga atctcctgag caaaatcttc
     7081 tcttcaaaag taaaaccggc aaaacattca tcttccagca tcttatctat ttcttctata
     7141 cctttcttta aaccccttcc cttttctgta aggtatacac gggaaactcg catatctcta
     7201 ggatcagtct ctcttctcag taagcctgcc ttttccatcc ttccaagcgt tacagttact
     7261 gtggatggtc taagctttaa tgattctgct atatcctttt gcgttaagcc atccttttcc
     7321 cataaaagtt ccagaatctg tggctgcccc ttatagatac caattttact cagaagatga
     7381 tgatgcctta taaaatgctc tcttatcacc tgccataaca atgagcctaa agaagtatca
     7441 tctttattat ccttacaaac catatatcat ctcctattat ttagatggct aaataatatc
     7501 acaaagatat tatgggtgtc aatagtgtct aaaggtagag aaatagaagg ataagaagaa
     7561 tatgtctctt attatttttt gttagacgtc tatatactgg aatcttctac tagagcattt
     7621 agtagcttaa cagcattttc tgcatcatct atatctatca tctcacaggg agaatgtaca
     7681 tatctgcaag gtatggatag agttccactt acgataccag cagaggtcct ctgaagtgcc
     7741 gctgcattcg ttcccccaaa tatcaatatc tcaatctgat agggtatgtt attggcttcc
     7801 gcaatattcc tgagtttatc cacaactctc ctgtcgctta tggaagcact gtccttaatc
     7861 tttatacatg gtcctttacc caattctata gacatcctct tatagccttt aggtgtatca
     7921 gaccaggcgg taacatccag tgctatagcc atttcagggt ttatattaaa tgccacaaca
     7981 ctggcaccga caagtcccat ctcttcctgg acagaaaaag tcccgtaaag tgtatggtat
     8041 ggatcagacc tctttatagc ttctataagg actgcgcaac ctatcctatc atccatagcc
     8101 tttgaaac
//
LOCUS       DAOTKU010000011         7684 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_9324_length_7684_cov_3.525888, whole genome shotgun sequence.
ACCESSION   DAOTKU010000011 DAOTKU010000000
VERSION     DAOTKU010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7684
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9324_length_7684_cov_3.525888"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /gene="ispH"
                     /locus_tag="P5310_00585"
     CDS             complement(<1..692)
                     /gene="ispH"
                     /locus_tag="P5310_00585"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HRU31893.1"
                     /translation="MAEYHILLAREYGFCFGVKRAIKLAEETIRSGIKIASLGPIIHN
                     PLEVERLKNMGLRVIGGIEELGPDESLLIRSHGIPLDLLNEIKRKNINIIDATCPLVA
                     RAQHMVEFLFSEGYKIIIIGEDGHPEVKGLVSYGGGNISVVSSPEAIPDITPNDKLGI
                     VGQTTYPVDKFVEIVKEITGRGWEIRVFNTLCDASRKRQGAVKELADQTDVIIVIGGK
                     NSANTRRLVDIA"
     gene            complement(692..1210)
                     /locus_tag="P5310_00590"
     CDS             complement(692..1210)
                     /locus_tag="P5310_00590"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HRU31894.1"
                     /translation="MNSALLIIDMLNDFVSPDGVLYSPLSARTIPHIKKQLEIARSNK
                     LPIFYICDSHLKTDKEFEKFPPHCVKGSWGARIIDELTPVDGERIIEKRRYSAFLGTD
                     LDISLKELGINELIITGVVTNICILYTSYDARMLGYEVIIPRSCVASFSEDIHKFVLS
                     ELENTLGVKITD"
     gene            complement(1207..2556)
                     /gene="dnaB"
                     /locus_tag="P5310_00595"
     CDS             complement(1207..2556)
                     /gene="dnaB"
                     /locus_tag="P5310_00595"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HRU31895.1"
                     /translation="MGIEERVRSNVPPHNLEAEESVLGAMLLSRDAIARVIEILDSRS
                     FYRKAHRDIYEAIMELFEKGEPVDIISVSDLLARKGLLESVGGYTYLSSLLDKVPTPS
                     NAAYHAKIVQEKAILRELIDAGIKIKELAEEEAEEVPVIMDRAEQLIFAVTQRKVFQY
                     FISLKEELFNVFELMEVRSKKHSYLSGIPVGFEEFDKLTGGLQYSDFIIIAGRPSMGK
                     TALCLNIAHHVGVKLKLPVAIFSLEMSSEQVAMRMLSAEAGIPYHKLRVGDLNEVEWR
                     RITTGINSLAEAPIYIDDSPDISVLEMRAKARRLKSEVGLSLIIIDYLQLMNSGARVE
                     NRVQEISKISRDLKAMSRELNIPVITLSQLSRAVEQRQDKRPQLSDLRESGAIEQEAD
                     LVLFLYREEYYNKDTPEKGTCEVILAKHRNGPTGTIKLGFNKEIMKFEDLRGGLIQV"
     gene            complement(2560..3003)
                     /gene="rplI"
                     /locus_tag="P5310_00600"
     CDS             complement(2560..3003)
                     /gene="rplI"
                     /locus_tag="P5310_00600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HRU31896.1"
                     /translation="MEVILLTDVPGLGREGDIKEVSEGYARNYLIPKGLAARSTRGRV
                     KDIELHKSAAAIKEVKEKEMAMKLAEKLKGVTLNFKVKVGEENKLFGSITSGDIAERL
                     NDKGFNIDKHQVVLKEPLKYLGTYEVIVKCGFGVNAPINVILESE"
     gene            complement(3006..3257)
                     /gene="rpsR"
                     /locus_tag="P5310_00605"
     CDS             complement(3006..3257)
                     /gene="rpsR"
                     /locus_tag="P5310_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010074384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HRU31897.1"
                     /translation="MAQDRRREGTRRFRGHQRKKVCAFCTEKIVIDYKDIDRLRHFLS
                     DRGKILPKRTTGTCARHQRKLARAIKRARIMALLPFTSD"
     gene            complement(3324..3752)
                     /locus_tag="P5310_00610"
     CDS             complement(3324..3752)
                     /locus_tag="P5310_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007505366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRU31898.1"
                     /translation="MMNHIVLTGRLTADPVLRYTPTGIPVLDFTLAVSRRGKSSEGER
                     DADFIDIVVWRKQAEICANYLKKGRLIGVEGRLEIDKFQGSDGQIHRKPRVVAYRVEF
                     LGSKPKEAEEGISEEIEGFDEDAFVDDISSKIDIDTEGNK"
     gene            complement(3752..4036)
                     /gene="rpsF"
                     /locus_tag="P5310_00615"
     CDS             complement(3752..4036)
                     /gene="rpsF"
                     /locus_tag="P5310_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006522188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HRU31899.1"
                     /translation="MRDYELMCIFNPDLEESKVEDIINRLQEIVNNNGGELQGIDRWG
                     KRRLAYPIKKFHDGYYIVMNFKGNGALLKELDRIIKLTEESLRHMIIRRD"
     gene            complement(4084..4455)
                     /gene="rplL"
                     /locus_tag="P5310_00620"
     CDS             complement(4084..4455)
                     /gene="rplL"
                     /locus_tag="P5310_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020155381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HRU31900.1"
                     /translation="MTKEEIVEAISQMSVLELSELVKALEDKFGVQAAAPVAMAPTAA
                     PVAEEKPKEQTEFDVIITSAGEKKVQVIKVVKDITGLGLKEAKDLVDAAPKAVKEKVS
                     REEAEQIKAKLEEVGATVEIK"
     gene            complement(4474..4998)
                     /gene="rplJ"
                     /locus_tag="P5310_00625"
     CDS             complement(4474..4998)
                     /gene="rplJ"
                     /locus_tag="P5310_00625"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HRU31901.1"
                     /translation="MALKEEKVLVVENTLEKLNKANGFIVAENRGLVIKDVETLRKNI
                     KAKNGEAKVIKNTLFNIALKKKGVNLNGSKSLLEGPNMFIFSYGDEIELLKEVIRFSK
                     SKEKLVPKGMFLEGRLYEGSQLEEISKLPPKEVLLAIVLGGIVGPIVSLVNVLQAPMR
                     DLLYVLNGIKEKKS"
     misc_feature    complement(5004..5109)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(5128..5835)
                     /gene="rplA"
                     /locus_tag="P5310_00630"
     CDS             complement(5128..5835)
                     /gene="rplA"
                     /locus_tag="P5310_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HRU31902.1"
                     /translation="MPKHGKKYTEVLKLIDRKKRYDVDEAISLVKQVSYARFDESVEI
                     AIRLGVDPRHAEQNVRGTVVLPHGIGKTRRVLVFARGEKAQEAVDAGADYVGAEDLVA
                     KINEGWTDFDVAIATPDMMGLVGRLGKILGPRGLMPNPKVGTVTTDIANAVRELKQGR
                     VEFRVDKFGIIHTVIGKRSFESERLVENFNVLMNAILRVKPSGVKGQYVRSVAISTSM
                     SPGIKVDPLRATKLSAV"
     gene            complement(5851..6276)
                     /gene="rplK"
                     /locus_tag="P5310_00635"
     CDS             complement(5851..6276)
                     /gene="rplK"
                     /locus_tag="P5310_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011056150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HRU31903.1"
                     /translation="MPKKVIGIVKLQIPAGKATPAPPIGPALGQYGVNIMEFCKSYNA
                     QTSGQAGEIIPVEITIFEDRSFTFILKTPPASELIKKALGIEKGSGEPNKNKVGKLTT
                     AQLKDIAQKKLQDLNTDNLEAAMRIIAGTARSMGVTVED"
     gene            complement(6292..6834)
                     /gene="nusG"
                     /locus_tag="P5310_00640"
     CDS             complement(6292..6834)
                     /gene="nusG"
                     /locus_tag="P5310_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014792372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HRU31904.1"
                     /translation="MTESKTEKGKWYVIHTFTGYEEKVKANLERKIRSLGAENKIFQI
                     LVPIENEIELHELQKKVVKRKIFPGYLLVNMEMDDETWMIVRSTPGVTGFVGIGGKPT
                     PIPEDELSTIMRRIKWEDVQYRTSLQVGDIVRIKYGPFEDFAGTVEEIIPERKKVRVT
                     VSIFGRETPLELRIDQVEKV"
     gene            complement(6842..7051)
                     /gene="secE"
                     /locus_tag="P5310_00645"
     CDS             complement(6842..7051)
                     /gene="secE"
                     /locus_tag="P5310_00645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HRU31905.1"
                     /translation="MQLSLFKRIWQKIKQFFREVRSELRKVTWPSRDEVKVLTIIVVL
                     FVAIFTAFIGVVDLILSRIVAFIAK"
     gene            complement(7129..>7684)
                     /locus_tag="P5310_00650"
     CDS             complement(7129..>7684)
                     /locus_tag="P5310_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRU31906.1"
                     /translation="NFDFIQAAHGRASSVATGVKRVYPDRVVFAYQGDGDMAGLGLGE
                     AMHTAVRGEKIAVVFINNAVYGMTGGQMAPTSLISQKTSTTPRGRDPESAGYPIRLLD
                     MMAVSEGCAFAARAALNSPKNVLKAKEYLRMAFQCQLDRKGYSIVEFLSTCPTNWRLD
                     TARSLKYIDEVMSSFYPPGIIKQA"
BASE COUNT         1957 a         1901 c         1190 g         2636 t
ORIGIN      
        1 tctgcgatat ctaccagtct ccgggtattg gcactattct taccgcctat aactattata
       61 acatctgttt gatctgccag ttctttcacc gctccttgcc tttttctcga tgcatcacat
      121 agagtattaa agactcttat ctcccaccct ctacctgtaa tctcttttac aatctctaca
      181 aatttatcta cagggtatgt ggtctgtcct acaatcccca gtttatcatt tggggttata
      241 tcaggtattg cttctgggga ggaaactaca gagatattcc ctcctccata actgaccagc
      301 cccttcactt ctggatgtcc atcctctcct ataatgataa tcttatatcc ctctgagaag
      361 aggaattcta ccatatgttg tgccctagca acaagtggac aggtggcatc tatgatattt
      421 atgtttttcc tctttatttc attcaggaga tccagtggaa ttccatggga ccttattaat
      481 aaggattcat caggacccaa ttcttctatc ccccctatta cccttagtcc catattttta
      541 agtctttcta cctctaaggg gttatggatt attggtccaa gagaggcaat ctttatccct
      601 gaccttatag tttcttcagc taattttatg gctcttttta ccccaaagca aaaaccgtat
      661 tctctggcta agagtatatg gtattcagcc attaatcagt aatctttacg ccaagggtat
      721 tttccagctc actcaataca aatttatgga tgtcttcaga aaaagatgca acacagcttc
      781 ttggaattat tacctcatat ccaagcatcc tggcgtcata tgaggtatac agaatacaga
      841 tattggttac aacccctgtt atgattaact cattaattcc caattctttt agagatatat
      901 caaggtctgt gccaagaaag gcactgtatc ttctcttctc gataattctt tctccatcta
      961 caggtgtaag ttcgtctatt atcctggctc cccagctccc ctttacgcaa tgtggaggaa
     1021 atttctcaaa ctctttgtct gtttttagat gtgaatcgca tatataaaag atgggcagct
     1081 tattacttct tgctatttcc aattgttttt ttatgtgggg gatggttctt gcagaaagag
     1141 gagaatagag aactccatcg ggggagacga aatcatttag catatctata ataaggagtg
     1201 ctgagttcat acttgaatca atcctcccct taaatcttcg aatttcatta tttctttgtt
     1261 aaatccgagc tttatcgtac cggtaggtcc attcctgtgc ttggcgagta taacctcgca
     1321 tgtccccttt tccggggtat ccttattgta gtattcttct ctgtataaga ataataccag
     1381 atctgcttcc tgttctatag ctccactctc acgaaggtca gaaagctgtg gacgtttatc
     1441 ctgtctctgt tctacagccc tgctcaactg ggagagagtt atcaccggaa tgtttaattc
     1501 tctggacata gccttcaggt ctcttgaaat ctttgatatc tcctgaactc tgttctccac
     1561 tctggctcct gaattcataa gctggagata gtctattata attagtgata gcccaacttc
     1621 agatttaagc cttctggcct tcgcccgcat ttccagcaca ctgatatctg gagaatcatc
     1681 gatgtatata ggagcttccg ctaaggagtt aattcccgtt gtaatccttc tccattcaac
     1741 ctcgttgagg tctcctactc taagtttatg gtaaggaatc cctgcttctg cagagagcat
     1801 cctcatagct acctgttccg aagacatctc taaactgaat attgctaccg gaagcttaag
     1861 ctttacccct acgtgatggg caatattcag gcagagtgca gtcttaccca tagacggtct
     1921 ccccgctata attataaaat cggaatactg aagaccacct gtaagtttat caaactcttc
     1981 aaaacctacc gggattccgc tgagataact gtgcttttta gaccttacct ccataagttc
     2041 gaatacgttg aaaagttctt ctttcagaga tataaagtat tgaaagactt tacgttgagt
     2101 tacagcaaat ataagttgtt ccgccctatc cataattaca ggaacctctt ctgcctcttc
     2161 ctctgcgagc tcctttatct ttatcccggc atctataagc tccctcagta tagctttctc
     2221 ctgtactatc ttggcatgat atgcagcatt ggaaggggtt ggaaccttat ccagcaggga
     2281 agagagatat gtataacccc ctacagactc taataagccc ttcctggcta ataaatcaga
     2341 gacacttatt atatctacag gttctccttt ctcaaagagt tccataattg cttcataaat
     2401 gtctctatga gctttacggt agaaacttct ggaatcaaga atctctataa ccctggcgat
     2461 agcatctctg gataagagca tagcgcctag gacagactct tctgcctcca ggttgtgtgg
     2521 ggggacgttg gatcttaccc tctcttcgat acccattatt tattcactct ctaaaattac
     2581 atttataggg gcatttacac caaatccaca ctttactata acttcatatg tccctaaata
     2641 ttttaatggt tctttgagga ctacctggtg tttatcaata ttaaagcctt tatcgtttaa
     2701 cctttcagct atatctcctg aagtaataga accaaaaagc ttgttctctt cccccacctt
     2761 tactttaaaa tttagagtga caccttttaa cttctcagcc agtttcatag ccatttcttt
     2821 ctctttaact tcttttattg cagcagcact tttatgaagc tcgatatctt ttactcttcc
     2881 tctggtagat ctggctgcaa gtcctttagg tataagataa tttctggcat atccctcaga
     2941 gacctctttt atgtccccct ctctacctaa tcctggtaca tccgttaaaa ggataacttc
     3001 catctttaat cactcgtaaa gggcaaaagc gccattattc ttgccctttt aatagctctg
     3061 gctaattttc tctgatgtct agcacaggtt ccggtggttc tctttggcaa tatctttcct
     3121 ctatcagaga ggaaatgcct caatctatct atatctttat aatcaattac aatcttctct
     3181 gtacagaatg cacaaacctt ttttctctga tgacctctga atcttcttgt tccttctctc
     3241 cttctatcct gagccatatt tctccctggg accctttaag ggtaggtgaa aatttaatat
     3301 tcgccttcac cttgagcgat actctatttg tttccctctg tatctatatc gatcttactt
     3361 gagatatcat ctacaaatgc atcttcgtca aaaccctcta tctcctcaga aattccttct
     3421 tcagcctctt tgggtttact acccagaaat tcaacgcggt atgctactac tctgggtttt
     3481 ctgtgaatct gcccatctga cccttgaaat ttatctatct ccagccttcc ttctacccca
     3541 atcagtcttc cctttttgag gtaattggca catatctctg cctgctttct ccacaccact
     3601 atgtctataa aatcggcatc cctctcccct tcagaggatt tccctcttct gctcactgct
     3661 aatgtgaagt ccagaactgg aattcctgta ggggtatatc tgagtacagg gtcagcggtg
     3721 agtcttccag ttaatactat gtgattcata attaatctct cctgattatc atatgcctta
     3781 gactttcctc ggtaagcttt attatcctat ccagctcttt taagagtgca ccatttccct
     3841 taaaattcat tactatatag taaccatcgt ggaacttttt aataggatag gctaaccttc
     3901 tcttacccca tctatctatt ccctgcaact ctcccccatt attattaacg atctcctgca
     3961 gcctgttgat aatatcttct acttttgatt cctctaggtc aggattaaaa atacacatta
     4021 actcgtagtc tctcaacttt ggttttttcc tcctattata ttagggataa agtagggtta
     4081 cccctacttt atctcgaccg tagctccgac ttcttccaat ttagccttta tctgctctgc
     4141 ttcttctctt gatactttct cctttacggc ttttggagca gcatcaacaa ggtctttagc
     4201 ctctttgagt cctaaacctg tgatatcctt tactactttt atgacctgaa ccttcttctc
     4261 accagcagaa gtaattatta catcaaactc tgtctgttcc ttaggcttct cctcagccac
     4321 cggtgctgct gtaggagcca tagccactgg agcagcagcc tgaactccaa acttatcttc
     4381 caaagccttt accagctctg aaagctccag tacactcatc tgggatatag cctcaacaat
     4441 ctcttccttt gtcatttcca tattacctcc ttcttaggat ttcttctctt taataccatt
     4501 cagaacatat agaaggtctc tcataggtgc ctgtaataca ttaaccagac ttacaattgg
     4561 accaactatt ccgcccaata ctatggctaa gagtacttct ttaggcggta gcttacttat
     4621 ctcttccagt tgagagccct catataatcg accttctaaa aacatcccct tagggacaag
     4681 cttttcttta ctcttagaga atcttatgac ctctttaagg agttcaatct catccccata
     4741 agaaaatata aacatattcg gtccctcaag gagggattta ctaccattaa ggttgacacc
     4801 tttcttcttg agggctatgt taaagagtgt attctttatg accttggctt ccccattttt
     4861 agcctttata ttcttcctca aagtttctac atcttttatg acaagccctc tattttctgc
     4921 cacaataaat ccgttagcct tgtttaactt ttctaaagta ttctctacta ccagtacctt
     4981 ttcttctttt aaagccattt tctcctccta taaaataacc cttccgcaag ataaggaagg
     5041 gtcgcccttc ctcggcagga tattaagcct ttcggcacct gctttcttag gaattataga
     5101 attataataa atcagcaata tttaccttta tactgcactt aattttgtag ctctaagtgg
     5161 gtctactttt ataccagggc tcatagaggt cgaaatagca acacttctca catactgccc
     5221 cttaactcca gatggcttta ccctgagtat tgcgttcata agtacattaa agttctctac
     5281 cagtctctca ctttcgaaag acctcttgcc tataacagta tgaattatac caaacttgtc
     5341 aaccctgaat tcaactctac cctgttttag ctccctgacc gcattagcta tatctgttgt
     5401 aactgttccc accttaggat ttggcataag acctcttggt ccaagaatct taccaagtct
     5461 tcctaccagt cccatcatat ccggggttgc tatggcaacg tcaaagtcgg tccaaccctc
     5521 atttattttt gccaccaggt cttcagctcc aacataatct gctcctgcat ctacagcttc
     5581 ctgagccttt tctcctctgg caaagactaa gactctcctg gtttttccta taccatgggg
     5641 tagtactacc gtacctctta cattctgctc tgcatgtctg ggatctaccc ccagtcttat
     5701 agcaatctct accgactcgt caaacctggc ataactcacc tgttttacaa gtgagatagc
     5761 ctcatccaca tcatacctct tttttctatc tataagttta agtacttctg tatatttttt
     5821 accgtgctta ggcatttttc ttccctctct ttaatcttct acggttactc ccatacttct
     5881 agctgttcca gctattatcc tcatagcagc ttccagatta tctgtattta aatcctggag
     5941 tttcttctgg gcaatatctt taagctgggc ggtggtaagt ttacctacct tgtttttatt
     6001 tggctctcca gaaccttttt ctattcccag cgctttcttt ataagttcag aggcaggtgg
     6061 agttttgaga ataaaggtaa aacttctatc ctcaaagatg gttatctcta caggaataat
     6121 ctctccagcc tgtccagaag tctgtgcatt gtaagattta cagaattcca ttatattaac
     6181 tccatactgt cccagggcag gacctatcgg aggggctggt gtagctttgc ccgctggaat
     6241 ctgtaacttt actattccga ttaccttttt aggcatctaa atactacctc cttatacctt
     6301 ctctacttga tctattctta attctaaagg cgtctctcta ccaaatatag agacagttac
     6361 tcttaccttt ttcctttcgg gaattatctc ttctaccgtc cctgcgaaat cttcaaaggg
     6421 accatacttt atcctaacta tatccccaac ctgcagggat gttctatatt gaacatcttc
     6481 ccattttatc cttctcatta ttgtcgaaag ttcatcttct ggtattggag taggctttcc
     6541 tccaatacca acaaaaccag taacacctgg agtacttctt actatcatcc atgtttcatc
     6601 gtccatctcc atatttacaa gaagataacc cggaaatatc tttctcttta ccaccttctt
     6661 ttgaagttca tggagttcga tctcgttctc tataggaaca agaatttgaa atatcttatt
     6721 ctctgcccca agagaacgaa tctttctctc taggttagct ttaaccttct cttcatatcc
     6781 agtaaatgta tgtattacat accacttgcc cttttctgtc ttactctcag tcataattca
     6841 cctatttagc tataaacgct acgattctag atagtataag gtctactact ccaataaatg
     6901 ctgtaaatat agcaacaaat aatactacaa ttattgttaa aaccttcact tcatctctgc
     6961 taggccaggt aactttcctt aattctgacc tgacctctct aaaaaactgc tttatctttt
     7021 gccatattct tttgaacaga gataattgca tctctcctcc ttctatatat cagaaactct
     7081 aacacatcta atataatacc agaaggattt ctttttgtaa agaatcctct aagcctgttt
     7141 tataattcca ggtggataga agctactcat aacttcatct atatatttca aagacctggc
     7201 tgtatccaac ctccaatttg taggacaggt ggataaaaat tctactatag aatatccctt
     7261 tctgtcaagt tggcactgga atgccattct tagatattct ttagctttta agacattctt
     7321 gggtgaattg agggcagctc tcgctgcaaa ggcacatcct tctgaaacag ccatcatatc
     7381 cagaagtctt attggatatc cggcactctc agggtctctc cctctgggag tagttgatgt
     7441 cttctgggat ataagacttg taggtgccat ctgtccacct gtcataccgt agactgcatt
     7501 atttataaat accaccgcta tcttttctcc ccttacagct gtatgcatag cttctcctag
     7561 acctaaacca gccatatctc cgtcaccctg ataggcaaat acaaccctgt caggatatac
     7621 tctttttact cccgttgcta cagaggatgc ccttccgtga gccgcctgaa taaaatcgaa
     7681 attg
//
LOCUS       DAOTKU010000012         7671 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_9338_length_7671_cov_4.100315, whole genome shotgun sequence.
ACCESSION   DAOTKU010000012 DAOTKU010000000
VERSION     DAOTKU010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7671
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9338_length_7671_cov_4.100315"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..790
                     /locus_tag="P5310_00655"
     CDS             <1..790
                     /locus_tag="P5310_00655"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRU31907.1"
                     /translation="LEDSYPPGMALELTHNFSLVHDDIQDKDLFRRGRETVWSIWGVA
                     QGINVGDAMIALSHRAILDSHLSDDKKCKLMDLLDNAIFSLCQGQFLDVSFESDDEID
                     REDYLLMVRGKTCSMFEASTGMGVVASGREELMDEFISIARNLGLAFQILDDILGLWG
                     DPNITGKPVGSDLRQNKKTYPIIIGIKEFPDLKGLILKKDKTDREIEYIRSTLEDYGI
                     RERCQREASGYLADARKTIEGLPLGRDEKDLLSELTYYLENRER"
     gene            830..906
                     /locus_tag="P5310_00660"
     tRNA            830..906
                     /locus_tag="P5310_00660"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:864..866,aa:Arg,seq:acg)
     gene            909..984
                     /locus_tag="P5310_00665"
     tRNA            909..984
                     /locus_tag="P5310_00665"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:942..944,aa:Arg,seq:cct)
     gene            complement(1181..3148)
                     /locus_tag="P5310_00670"
     CDS             complement(1181..3148)
                     /locus_tag="P5310_00670"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRU31908.1"
                     /translation="MNKGFYRVFLCLVICMLLISNVPVSFAQKFPDIPQYTTPAEYEK
                     ATGKKITKLNEASQLAELVKQGKLPSVEQRLPKEPLVVVPVEEVGKYGGVWRTAMLGS
                     ADTMHLAREVGYEPLVRIDGSFSKVIPGVAKSWTVSKDGKIFTFYLREGMKWSDGEAF
                     TADDIMFWYKDILLNKELTPVISKQLTSDGKPVKVKKINDYTVRFTFTQPQGLFLPWL
                     STGLKPFAPKHYLKDFHPAYAPVDKLNDMAKKAGFDYWYQLFAVKNDPWMNPELPVIH
                     AWKVVTPLGAGTRVSFERNPFYWKMDIAGNQLPYIDRFTADIVDSLEVMIMKTVSGEI
                     DCQHRHIGQSTQDYPFLMEQRSKGDYRILTQQHASAVVASVFLNLNHKDPIVKKIFND
                     IRFRQALSLAINRKEIIDLVYLGKPEPWQISPKKGTPLYYEKGSKAYIEYDPSRANQL
                     LDRMGLKKGSDGWRLRPDGKQLTITIEIGRTALGWPDAAEMVKKYWENVGVKTVVKVE
                     DRSIFTARYQGAEHDAVIWAGEGSAGLEPLLNPWWYVPVSRGAYYAPLWGLWYETKGQ
                     SGEEPVPIVKRQMQLYDKLLNTTNSEKQLELMREIIKLAADNLWHINICTYPDEHWIV
                     KNNFRNVPDNSITSGMFPNSGPYNPCQFFIK"
     gene            complement(3478..4395)
                     /locus_tag="P5310_00675"
     CDS             complement(3478..4395)
                     /locus_tag="P5310_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004397268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRU31909.1"
                     /translation="MRYMNIGNEKIKASEIALGCMRIADKPVKEVAELINIALDEGIN
                     FFDHADVYGRGKCEEIFAEATGMNSNIREKFIIQTKCGIRRGYYDFSKEHILEAVDGS
                     LKRLNTDYIDILLLHRPDTLMEPEEIAEAFSILHSSGKVRNFGVSNFHPMQIELVSKY
                     LNQRIIVNQLQLSIAHTGMIDFGFNVNMKVDQAINRDGGILEYCRVKDITIQAWSPFQ
                     YGFFEGTFMGNSKFQELNDKIEEIAREKGIPNIAVPIAWILRHPARIQPIVGTTNPSR
                     LKDICKASGVELTKEEWYELYRSAGNKLP"
     gene            4673..5026
                     /locus_tag="P5310_00680"
     CDS             4673..5026
                     /locus_tag="P5310_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU31910.1"
                     /translation="MSFFTDGRGKKIDIPDDTNWYHGSPEVIDVLAAGSSITRNKQLA
                     IAFSHKPSMICIDDEGGLNHDGECNGYLYVIDEPVNADDIYAHQACAKDDLWEWITRR
                     PLKVKKIEDTRVVQP"
     gene            5133..5405
                     /locus_tag="P5310_00685"
     CDS             5133..5405
                     /locus_tag="P5310_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU31911.1"
                     /translation="MQRKQVNWKSCRIIDNAAQRLLDKVKEAVQGFLENTDDKGTVVR
                     WIAAFTLTEIAKSSKDVRKRLVPEFKKMPEREDKSGVGNILKYLKT"
     gene            5421..6035
                     /locus_tag="P5310_00690"
     CDS             5421..6035
                     /locus_tag="P5310_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011021157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HRU31912.1"
                     /translation="MKKIDLKKELKHLYNPPKEIVFVDVPEMSFLMIDGAGDPDTSRE
                     YKDAIEALYAVSYTLKFMVKKENEVDYVVMPLEGLWWTEPISQFTMENKDVWKWTAMI
                     MQPEYVTEELFTRAAEQVKIKKNLASLSKIELKSLHEGLSVQLMYIGSYSAEGPTIEK
                     LHNFIKENGYELRGKHHEIYLSDPRRSAPEKLKTVIRQPVKREQ"
     gene            6032..6517
                     /locus_tag="P5310_00695"
     CDS             6032..6517
                     /locus_tag="P5310_00695"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HRU31913.1"
                     /translation="MIDDVRGIIERGLHSLNDVLSEIRKLASYDDWRKREDAATALVE
                     ISKKKEDEVVNEMTLWVEDKDPNIRRVASEGLRGVARRNPEKILSVIEKLKTDDSLYV
                     RKSVAALLRAISKKDPDLVIDLCKKWTGLKNRNTNWIIKQGIKKLSEEQQEKIRALLD
                     E"
     gene            6519..7406
                     /locus_tag="P5310_00700"
     CDS             6519..7406
                     /locus_tag="P5310_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRU31914.1"
                     /translation="MVNEIQVKSILNKHKKRDNWFLDDYSLNPYSGCSFNCIYCYIRG
                     SKYGENMARALSVKVNAAELLEKQLSRRAKKGEYGIIVLSSSTDPYMPIEEKLELTRK
                     LLEIILKYKFPVEVATKSKLVLRDLDLLKEIDKNAILPDDLKPKLKHGAIISFSIPTL
                     DERLAETLEPGAPEPIERLETMRKCKEEDFLTGVCLIPVLPFLSDSEEQLEETIRTVK
                     EYGADFIFVGGLTLSGKGPADCKTLYYKFLEKHYPELVPKYKSLYRIFFAPSKEYQKE
                     LEEKSERLCKKYGIKNRII"
     gene            complement(7541..7671)
                     /locus_tag="P5310_00705"
                     /pseudo
     CDS             complement(7541..7671)
                     /locus_tag="P5310_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007301911.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2365 a         1459 c         1554 g         2293 t
ORIGIN      
        1 tttagaagat tcgtatccac ccggtatggc tcttgagctg acccataact tttcccttgt
       61 tcacgatgat atacaggata aagacctctt cagaaggggt agagaaaccg tctggagtat
      121 atggggtgtg gctcagggta taaacgtcgg agatgctatg atcgccctct cccacagggc
      181 tatactcgat tctcatctga gtgatgataa aaaatgtaaa ttgatggatt tacttgataa
      241 tgcgatattc agtctctgtc agggacaatt tctggatgtg agctttgaga gtgatgatga
      301 aatagataga gaagactatc ttcttatggt tcggggtaaa acctgcagta tgtttgaggc
      361 aagtacaggt atgggtgtag ttgcatctgg cagggaagaa ctgatggatg aatttatatc
      421 tatagcgaga aatcttggac tggcatttca gattctggat gatatacttg gtctctgggg
      481 agatcctaat attacgggga aacctgttgg aagtgacctg agacagaata aaaagacata
      541 tcctattatc attgggatta aagaatttcc tgacctcaag ggtcttatcc ttaaaaagga
      601 caagaccgat agagagatag aatatatcag atctaccctt gaagattacg gtataagaga
      661 gaggtgtcag agggaagcct ccggatatct tgctgatgcc agaaagacga tagaaggact
      721 tcccctgggg agagatgaaa aagacctgct ttcagaattg acatactatc ttgagaatag
      781 agaaaggtag gttgaatatc caggacataa tgtgttagaa tatatatgtg cgcccgtagc
      841 tcaattggat agagcggcag actacgaatc tgcgtgttga aggttcgagt cctttcgggc
      901 gtgccagtgc cctagtagct caatggacag agcaggggac tcctaagcct ctggttgggg
      961 gttcgattcc tccctagggc accatatttt atttgtataa catacacaat aacttggtgc
     1021 accgggtaaa aaattccatt tatcgatttt tataaagtat atcttacttc aatcagggtt
     1081 gtaacttatg ggattcagag ataactaaag ccagcccttt cttaacatcg aagaaggctg
     1141 gctttatcga ataacctgat gaattattgg taaaatctta ttattttata aagaattgac
     1201 atggattata aggtccagaa ttaggaaaca taccactggt aattgagttg tccggcacat
     1261 tgcggaaatt gttttttacg atccagtgtt catcaggata agtacagata ttaatatgcc
     1321 agagattatc tgctgctaac ttaattatct cccgcattaa ctctaactgc ttttctgaat
     1381 ttgtagtatt tagaagttta tcgtacagtt gcatctgtct ttttactata ggtaccggtt
     1441 cttctccaga ttgtcccttt gtctcatacc agagtcccca gagaggggca taataagctc
     1501 ctctactcac cgggacatac caccagggat tcaatagtgg ttccaatcct gcactacctt
     1561 ctccagccca tataactgca tcatgttctg ccccctgata tctggcggta aatatagaac
     1621 gatcttcaac cttgacaaca gtttttacac cgacattttc ccaatatttt ttaaccatct
     1681 ctgcagcatc tggccaccca agagcagttc tacctatctc tatagttatg gttaattgct
     1741 tgccatctgg acgtaatctc catccatctg aacctttttt aagtcccatc ctgtccagta
     1801 gctgatttgc tctacttgga tcatattcta tatatgcctt ggatcctttt tcataataaa
     1861 gtggtgtacc tttcttaggt gaaatctgcc agggttctgg ttttcctaga tatacaagat
     1921 caattatttc cttacgattt attgccagag aaagtgcctg acggaatctt atatcattga
     1981 atatcttctt gactatcggg tctttatgat tgagatttag gaagacggag gctacaactg
     2041 cgctggcatg ctgctgcgtg agaatcctgt aatctccttt agatctctgt tccatcaaga
     2101 atggataatc ttgcgtactc tgtcctatat gtctatgctg gcaatctatc tctccactta
     2161 cagtcttcat aatcataacc tctaaactgt ctacaatatc tgcagtaaac ctgtcaatat
     2221 atgggagttg attcccggct atatccatct tccaatagaa tggattacgt tcaaaagaaa
     2281 ccctggttcc tgcccctaat ggggtaacaa ctttccatgc gtggataact ggcaactcag
     2341 gattcatcca tgggtcattt ttaacagcaa aaagctgata ccagtaatca aaaccagcct
     2401 tctttgccat atcattaagt ttatctaccg gcgcataagc aggatgaaaa tccttaagat
     2461 aatgcttggg tgcaaaaggt tttagccctg tagataacca gggtaagaat aacccttgag
     2521 gctgagtaaa ggtgaatctg acagtataat catttatctt tttaaccttt accggtttgc
     2581 catcacttgt aagctgttta gaaataacag gggtgagttc cttatttagc aggatatctt
     2641 tataccagaa cataatatcg tctgctgtaa aagcttctcc atctgaccac ttcatcccct
     2701 cgcgtagata gaaggtgaaa attttaccat cttttgaaac agtccagctc ttagctaccc
     2761 ctggaattac tttggagaag gatccgtcaa tccttaccag tggctcatag cctacctcac
     2821 gggctagatg catggtatct gcactcccta acatagcggt acgccatact cccccatact
     2881 ttcctacctc ctcaacaggc acaacaacca gcggctcttt aggaagtctt tgctccacag
     2941 atggtaattt gccctgtttt accagttcgg ctaactgtga agcctcattt agctttgtta
     3001 tctttttccc tgttgccttt tcatattctg ctggagtggt atattgaggg atatcaggaa
     3061 atttttgagc aaaagagaca ggaacattac tgatgagcaa catacatatc actaaacata
     3121 aaaatacccg ataaaaacct ttattcattt ataaccctcc atttcgaaaa ttctaaatct
     3181 ttttattctt actcactaag ccagattctt tgacctttat ccccttgcct ttgcttctaa
     3241 acatcacctc tctttctcat tgaacttttc aagtgcgcct ctctgaatct tttaacccct
     3301 cttaaattat accatacgca cattcattta tattttagcc cttctcctga agagctgtca
     3361 aattgtttgt taacgttctg tgatattgtc gggtatggat gcataacgca ccacctgttc
     3421 tgtaactttt acaagatcta aacattacac ctgatactga tctatcccct ttttattcta
     3481 ggggagttta ttccctgcag acctgtatag ttcgtaccat tcttcttttg taagctcaac
     3541 ccctgaggct ttgcatatat cctttaatct tgatggattt gtggtgccaa caattggctg
     3601 tatcctggct ggatgtctca agatccaggc aatgggcacg gcaatattgg gtataccctt
     3661 ttccctggca atctcttcaa ttttatcgtt aagctcctgg aatttactgt tccccataaa
     3721 tgtaccttca aaaaatccat actggaatgg tgaccatgcc tgaattgtaa tatcttttac
     3781 tctgcaatac tctagtatac caccatctct atttatagcc tgatccacct tcatattcac
     3841 attaaaacca aagtcgatca tccctgtatg tgctatactg agctggagct ggtttacaat
     3901 gattcgctga ttcagatact tgctgaccag ttcaatctgc atcggatgga aattgcttac
     3961 accaaaattt ctgaccttac cactgctatg gagtattgaa aaggcttcag caatctcttc
     4021 aggttccatt aaggtatcag gacggtgtaa caataagatg tctatatagt cagtattaag
     4081 cctcttcaga cttccgtcaa ccgcttctag tatatgttct tttgaaaagt cgtagtatcc
     4141 tcttctgata ccacatttag tctggatgat aaatttctct ctgatatttg agttcatccc
     4201 cgtagcttca gcaaatatct cttcacattt tcctctacca tatacatcgg catgatcaaa
     4261 aaagtttatt ccctcatcaa gtgctatatt tatgagctct gctacttctt ttactggttt
     4321 atcagcgatt ctcatgcatc ccagtgcaat ctctgatgcc tttatcttct cattaccaat
     4381 gttcatgtat ctcatttaag acaccttctt tctttaaaag tgaacttcca gaatctattt
     4441 tagcacctga taactttatc ggcaataaat tctggtagca tttatatgtg caggatcagg
     4501 agctagccaa atttttctaa gatgagtaag atttgtcgct aattaaatga agaaggttaa
     4561 agacagggca tagtgcttga caggtgaagg ggtcatcact ataattttag ctgaagagaa
     4621 ggcaaaaaag tgatttcgca agcaaaacat tgaagaaagg agttaacagg ggatgagctt
     4681 ttttacagat ggaaggggta aaaagatcga tataccagac gacactaact ggtatcacgg
     4741 ctctcctgaa gtcatagatg ttttagctgc tggaagttcg attacgagaa ataaacaact
     4801 ggcgatagct ttttcccata aaccatctat gatttgcata gatgatgaag gtggtttgaa
     4861 ccatgatgga gaatgcaatg gatatttata tgtgattgat gaaccggtca atgcggatga
     4921 tatttatgca catcaggcat gcgcaaagga tgatctatgg gaatggatta ccagaagacc
     4981 tctgaaagta aagaagatcg aggatactag ggtggttcaa ccataatgac cttaaagact
     5041 tttgtgggtg gcatatcgcg aaaaacgtgg cattcccact ttagtcgcgg gaaacagtta
     5101 aagggggctg atctgctata cctgttcagt aagtgcagcg taaacaggtg aattggaaat
     5161 cttgcagaat aatagataat gctgcccaaa gacttctaga taaggttaaa gaagcagtcc
     5221 agggcttttt agagaataca gatgataaag gaacggtggt aagatggatt gccgctttta
     5281 ctctcactga gatagcaaaa agtagtaagg atgtgcgaaa acgacttgtt ccagaattta
     5341 agaagatgcc ggaaagagag gacaagagcg gtgtggggaa tatattaaaa tatttaaaaa
     5401 cttgacagag gagatatatt atgaaaaaga ttgatttaaa gaaagaactc aaacacctgt
     5461 acaatccgcc aaaagagatt gtattcgttg acgttcccga gatgagtttt ttaatgattg
     5521 atggagcagg agacccagat acatcacggg aatataaaga tgcgatagaa gctttatatg
     5581 ctgtatccta tactctaaag tttatggtca aaaaagaaaa tgaagtagat tatgtcgtta
     5641 tgccgcttga aggtctctgg tggacagagc caataagcca atttactatg gaaaacaaag
     5701 acgtatggaa atggacggca atgattatgc aaccggagta tgtgacagaa gaacttttta
     5761 ctagagctgc cgaacaggtc aagatcaaga aaaatctagc ctctctttct aaaatagagc
     5821 tcaaaagtct gcatgaagga ttgtctgttc aattaatgta tattggttca tattctgctg
     5881 aaggtcctac cattgaaaaa ctccataact tcattaaaga aaatggttat gaattaagag
     5941 gaaaacatca cgagatatat ctgagcgatc cccgcagatc agcgccagaa aagctaaaaa
     6001 cagtgataag acagcctgtt aagagagaac agtgatagat gatgttaggg gtataattga
     6061 aagagggctc cattccttga atgatgttct ttctgaaata aggaaacttg catcatatga
     6121 cgactggagg aaaagagagg atgcagccac tgctttggtg gagatcagta aaaagaaaga
     6181 ggatgaagtt gtaaatgaga tgaccctctg ggttgaggat aaagacccta acattcggag
     6241 agtagccagt gaaggattaa ggggagtggc acgaaggaac ccggagaaga ttttatctgt
     6301 aatagaaaaa ctaaaaactg atgatagcct ttatgtcaga aagtctgttg ctgctctttt
     6361 aagggcaata agcaagaaag accctgacct tgtaatagat ttatgcaaaa aatggacagg
     6421 actaaaaaat aggaatacaa actggataat aaaacaaggc ataaagaagc tatcagaaga
     6481 gcaacaagaa aagataagag ctttattgga tgaatagaat ggtaaatgaa atccaggtaa
     6541 aatcaatcct gaacaagcat aaaaagagag ataactggtt tttagacgat tattctttga
     6601 acccatattc tggttgttct ttcaattgca tttactgcta tataagggga agtaaatacg
     6661 gggaaaatat ggcaagagca ttatcggtaa aagtaaatgc tgccgaactt ttggaaaaac
     6721 aactttcaag aagggcaaag aaaggagaat atggaattat tgtcctttca tcttcaaccg
     6781 acccatatat gcctattgaa gaaaaactgg aattgacaag aaaattgctt gagataattt
     6841 tgaagtataa atttcccgtg gaagtggcaa cgaaatcaaa acttgtttta agggatctgg
     6901 atttattaaa agagatcgat aagaacgcaa ttttaccaga tgatttaaag cccaaattaa
     6961 agcatggtgc aatcatttct ttttctatcc caactcttga tgagagattg gctgaaactc
     7021 ttgaacctgg agcacctgaa cccatagaaa gattagaaac aatgaggaaa tgcaaagaag
     7081 aagacttttt aacaggagta tgtctcattc ctgtattgcc atttttatcg gattcagaag
     7141 aacaacttga ggaaacgata agaactgtaa aagaatatgg agcagatttt atctttgttg
     7201 gtggattgac attatctggt aaaggtccag cagattgcaa gactctctac tacaaattcc
     7261 tagaaaagca ttatccagaa cttgtgccaa aatataagag tttatatagg atcttttttg
     7321 caccatcaaa agaatatcag aaggaacttg aggagaaatc agagaggttg tgtaagaaat
     7381 atgggattaa aaacagaatt atataacagc ggcttcgggg ttgtcaagat atctgtgtaa
     7441 acctcccagg tgtcatttta tagataccct cctagcctat cctcaaactg tatcgatagt
     7501 tctgccagga tcaagtgcca gtctcttatc ttacttgtcc aatctgactg tttaggctct
     7561 ctatcggatt tttagtatat attagccttc ttatctgtgg tgagtactta aagaacgttg
     7621 aaaggttatc ccagctatcc ctccatgact tccctatgta tgggtacttc t
//
LOCUS       DAOTKU010000013         7633 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_9386_length_7633_cov_3.805094, whole genome shotgun sequence.
ACCESSION   DAOTKU010000013 DAOTKU010000000
VERSION     DAOTKU010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7633
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9386_length_7633_cov_3.805094"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..686
                     /locus_tag="P5310_00710"
     CDS             <1..686
                     /locus_tag="P5310_00710"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRU31915.1"
                     /translation="GVIVTATPNLKDWAGTPLKDIIEDRFNLPTYVDNDGNVAALGEW
                     WIGGGRGARCLLCLTIGTGIGSGIVYEGKVFRGAKGVGAELGHMSIKYDGIRCNCGGI
                     GCIEAYASGPALMKKLQEKGKFFETPDEIQQAAESGDRIVLEAVKEIGALLGYAMVSF
                     INIFNPDIILLSGGVSNLGDFLIGPVRDIVDTYALPGGRDVKIGRAVLGENAGVVGAA
                     ALVYLKGAK"
     gene            708..1160
                     /locus_tag="P5310_00715"
     CDS             708..1160
                     /locus_tag="P5310_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020888282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HRU31916.1"
                     /translation="MAEERQLVVFSLLNENYGIDIYKVQEIIQYRDITYVPKSPPFVK
                     GVINLRGRIIPVIDLKERFGLPEKEVTPDTRIIVVEISSQTIGLIVDSVTEVLRIPNT
                     NIEPPPPVTTIEADFIEGVGKLDERLIIILDIDRILTKEEKRELAGIE"
     gene            1198..1866
                     /locus_tag="P5310_00720"
     CDS             1198..1866
                     /locus_tag="P5310_00720"
                     /inference="COORDINATES: protein motif:HMM:NF016365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HRU31917.1"
                     /translation="MNTATVITGSTIGLILGNRFPERLQKIAIQSIGLFTLVVGLSMG
                     LAAKDIKESIIVLVSLTLGGITGEILNIEGFLEKIGDSLSKKSSVKAGAFTQGFVAAS
                     LLFCVGPMAITGAIEDGIKGTYSILLTKSIMDGISSIALSASLGIGVIFSSLVVLGYQ
                     GILTILASIAGGILPEAAINCITATGGLMVIGIGINLLGLGKIKVGNLLPGLLIGGIL
                     GGIL"
     gene            1863..3119
                     /gene="serS"
                     /locus_tag="P5310_00725"
     CDS             1863..3119
                     /gene="serS"
                     /locus_tag="P5310_00725"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HRU31918.1"
                     /translation="MIDIRLIEIKPEEVRKLLKKKGVDAPIDEILRLNQERKDLIKEV
                     DSLRHLQNVTSREIQKNPDNKEELIAQVKGIVPKLRELEDKLKNIESSINELILSLPN
                     LPHESVPEGKDETGNIPIKYSGSIPQFDFVPKDHLELGESLDLIDVKRAGKVSGSRFY
                     YLKRELVILEYALVQFVTELLVKKGFIPIIPPVLVMEDILYGLGYLPELEEQMYKIPL
                     DKLRLAGTSEHTVVPMFKDEILDVKDLPQRFISYSSCFRREAGAAGKDTRGILRVHQF
                     DKLEMVSFTLPDKSWEEHEFLLSIEEEIMTRLNIPYRVVLICAGDLGFPASKKYDIEA
                     WLPGQDRYRETHSCSNCTDFQSRRLNIRYRDDDGNVHYVHTLNGTALAIGRALIAIME
                     NNQNSDGSINVPEVLIPYAKFEVIKR"
     gene            3116..4648
                     /gene="guaA"
                     /locus_tag="P5310_00730"
     CDS             3116..4648
                     /gene="guaA"
                     /locus_tag="P5310_00730"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013432699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HRU31919.1"
                     /translation="MKETVIVLDFGSQYTNLIARRIREQQVYSEILPCDIPFDEIEKI
                     NPKGIILSGGPSSVYEENAPKCSPLIFSGRYPVLGICYGMQIMAHMLGGKVIKGDKGE
                     FGKTLLKVEYNSVLFDGLDREFIVWMSHNDIVIKAPENFRVIASTDNTPIAGIEDDEK
                     KIYGIQFHPEVIHTINGDKIIKNFLYKVCSCSGDWTPEHFINDTIDSIRKTVNNGKVI
                     CALSGGVDSTTTAVLVHKAIGDNLICIFVNQGTLRENEVDQVLDTLKSFNMRVEYIDA
                     TDRFLGRLNGITDPEEKRRVIGHEFVNVFEEAAARIGDIDFLAQGTLYPDVIESATSS
                     QKAHKIKTHHNVGGLPEKMRLKLVEPLRNLFKDEVRRVALSLGIPEKIVYRQPFPGPG
                     LAIRVIGEVTKERLEIIRKADHIISAEIEKAGLNKELWQYFGILLPEMSVGVMGDRRT
                     FGHVVAVRAILSEDGMTADWARLPYSLLETISNRIINEVPKVNRVVYDISSKPPATIE
                     WE"
     gene            4668..6314
                     /gene="argS"
                     /locus_tag="P5310_00735"
     CDS             4668..6314
                     /gene="argS"
                     /locus_tag="P5310_00735"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HRU31920.1"
                     /translation="MILKQYIEDRVLDVLIKSDINNIEVVVDRTQNPEFGHYTTSTPF
                     NLSKILKKSPYDIAREIAEKIQDPLALIDKIEIKGGFINFFLNTDAFHLVVEDILEKK
                     ERYGRFNEGQGKKVQVEFVSANPSGPLHVGNGRGGALGDVLANIFEWFGYNVEREFYV
                     NDMGNQMNLLALSVEARMRELLGEDFEIPQEGYHGEYVKDIARSALEKFGNVIWNLSE
                     EERLKELKEFAEETILSWQREDLALFGIRFDCWYRESTLHKNNTLGEALEILKSKGYT
                     YERDGAIWFASTRFGDEKDRVLVKSDGSSTYFLADIAYHLDKYKRGFSHIIDIWGADH
                     YGYVARMKASVEALGYDPDTFEPIIYQIVHLFRGGSAVRMSKRTGEMVSLRELLDEVG
                     KDAARYFFVMRDPNSQLDFDIELAKSQSINNPVYYVQYAFARCCSLFKQAELKGIDIG
                     RIDHCDLSCLQDPLERDIMMILEDFPDMLKNVFYNRAPHRLTAYLESLAKGFHSFYNS
                     LRIIQENEDLMYARMALVSATRQVIKNGLDILGVSAPEKM"
     gene            6365..6712
                     /locus_tag="P5310_00740"
     CDS             6365..6712
                     /locus_tag="P5310_00740"
                     /inference="COORDINATES: protein motif:HMM:NF015721.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HRU31921.1"
                     /translation="MESRLENPLGQVSISANAIANIVTNATLRCPGVAGMSTKSFTEE
                     IANLLKVESPSRGVEINISEDKVNVNVFVLVSYGTNIPELADLIASNIKSDLEKYTGL
                     IVASVNVNVVGIS"
     gene            6733..>7633
                     /locus_tag="P5310_00745"
     CDS             6733..>7633
                     /locus_tag="P5310_00745"
                     /inference="COORDINATES: protein motif:HMM:NF028500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecG"
                     /protein_id="HRU31922.1"
                     /translation="MSLDIPVQYVKGVGPKRATLFGRLGVETLRDLLYFAPKRYEDRS
                     NLSPINSLESDTYQTVFCQVVDVKEERVRKNLTLIKARVRDDTGEAIAIWFNQTYLKN
                     ILKRGVKLYLYGNISRKYGRIEIQNPDYEIADGDETIHTGRIIPVYSLTEGLSEKIVR
                     NIIWNTLNNYLEELEEFLPEEILDKYGFPDIKRAIWGIHFPDDEVTLRKSRERLTFEE
                     FFILQLIILRRRMSLLKEYAPVIKVSRNLEKRFLESLPFELTNAQKRVIEDIKRDLAS
                     GKSMQRLLQGDVGSGKTVVAAFFL"
BASE COUNT         2417 a         1112 c         1874 g         2230 t
ORIGIN      
        1 ctggagttat agttacggct actcctaacc ttaaagactg ggctggtaca cctcttaaag
       61 atataataga ggatagattt aatctgccaa cctatgttga taacgatggt aacgtggcgg
      121 ctttgggaga gtggtggatt ggaggaggta gaggtgccag atgccttttg tgtctcacta
      181 taggaaccgg aattggttca gggattgtgt atgagggtaa ggtgtttaga ggagcgaaag
      241 gggttggtgc cgagttgggt catatgagca ttaagtacga tggtattagg tgtaactgtg
      301 ggggtatagg atgtatagaa gcctatgctt ctggtcctgc acttatgaaa aagcttcagg
      361 aaaagggtaa gttttttgaa actccggatg agatacagca ggctgctgag tctggagata
      421 gaatagtact ggaagcagtt aaagagatag gtgcactgct tggttatgct atggtaagct
      481 ttatcaatat atttaatcct gatattatac tcctctctgg tggagtatct aatctggggg
      541 actttttaat tggtcctgta agagatatag tggatactta tgccctacca ggtggtagag
      601 atgttaaaat aggaagggca gttttgggtg aaaatgcagg tgtggttggt gccgctgctc
      661 ttgtatatct taaaggggca aaataattaa aaaggaggaa aaagagtatg gcggaagaga
      721 gacagttagt agttttttct cttttaaatg agaattatgg aatagatata tacaaagtac
      781 aggagataat tcagtataga gatataacct atgtccctaa gtcgccacct tttgtaaaag
      841 gagtgataaa tctcagggga aggataatcc ccgttataga tttaaaagag aggtttggac
      901 ttccagagaa agaagtcacc ccagatacca ggataatagt tgtggagatt tcgtctcaga
      961 ccattggtct tattgttgac tctgtaaccg aggtcttaag aatcccaaat actaacatag
     1021 aaccacctcc tccagttacc acgatagaag cagattttat agagggcgtg ggtaaactgg
     1081 atgaaagact cattataatt ctggatatag acaggatatt aacaaaagaa gagaagagag
     1141 aactggcggg tatagaatga cctttacagg tggtgattta atattttagg gacatttatt
     1201 aatactgcaa ctgttataac aggaagtact attggtctta tactggggaa caggtttcct
     1261 gaaagattac agaagattgc catccaatcg ataggtctgt ttactctggt agtaggatta
     1321 agtatggggt tagctgctaa agacataaaa gagtctatta tagtactggt aagtcttact
     1381 ttagggggta taacaggcga aatattgaat atagaaggat tcttagaaaa aataggggat
     1441 tctctaagta agaagtcttc tgttaaagca ggtgccttta cccagggttt tgtggctgcc
     1501 agtttacttt tttgtgtggg acctatggct atcactggag ccatagagga tgggataaaa
     1561 ggaacctaca gtatattact taccaagtct attatggatg gaatttcttc catagcttta
     1621 tctgcctctt tagggatagg agtaatattt tcttctctgg tagttttggg ataccagggg
     1681 atattaacga ttctggcttc tatagctgga ggaattcttc ctgaagcggc gataaattgt
     1741 atcactgcaa ctggtggatt gatggtcatt gggataggaa taaatctact gggattggga
     1801 aagattaaag tgggaaattt actcccagga cttttgatag gtggaatatt aggaggaata
     1861 ctgtgataga tataagactt attgagataa aaccagagga agtaaggaaa cttctgaaga
     1921 aaaaaggagt agatgctcct atagacgaaa tattaaggtt gaatcaagag aggaaggatt
     1981 taattaaaga agtagatagt ttaagacact tgcagaatgt aacatccaga gagatacaga
     2041 agaatcctga taataaagag gaattaatag ctcaggttaa aggaattgtt ccaaaactca
     2101 gagagctaga ggataaatta aaaaatatag agtcctccat taatgaatta atcctctctt
     2161 tgcctaatct tcctcatgaa agcgtccctg aagggaaaga tgaaactggt aatataccga
     2221 taaaatattc tggtagcatc cctcagtttg attttgtccc aaaagaccat ttggaactgg
     2281 gagaatcgct ggacctgatt gatgtaaaac gagctggaaa ggtaagtggt tcaaggttct
     2341 attatcttaa gcgagaatta gtaatattag aatatgcact tgtacaattt gtgactgaat
     2401 tactggtaaa aaaaggtttt attccgatca ttcctcctgt gcttgttatg gaggatatac
     2461 tgtatggact tggttatttg cctgaacttg aagaacagat gtataaaatt cctctggata
     2521 aattaagatt agcagggaca tctgaacata cagttgtccc catgtttaaa gatgagatac
     2581 ttgatgtaaa agaccttccg cagagattta tctcatattc ctcctgtttc agaagagaag
     2641 ctggtgcagc tggaaaagat acacgtggaa tattgagagt ccatcagttt gataaattgg
     2701 agatggtctc ttttaccctc cctgataagt cctgggaaga gcacgaattt ctcctctcaa
     2761 tagaggaaga gattatgacc agacttaata tcccatacag ggttgttctt atatgtgcgg
     2821 gagatctggg attcccggca agtaagaaat acgatataga agcatggcta cctggtcaag
     2881 atagatatag agagacgcac tcctgcagta actgtaccga ttttcagtcc agacgcttga
     2941 acataagata tagggatgat gatggaaatg tgcactatgt acatacttta aatggtaccg
     3001 cactggctat tggtagagcc cttatagcta ttatggagaa taaccagaat agcgacggaa
     3061 gtataaatgt cccggaggta ctgattccat acgctaaatt cgaggtaata aagagatgaa
     3121 agaaacggtt atagttcttg attttggctc tcaatatacc aatcttatag ctcgaagaat
     3181 aagagaacaa caggtctact ctgagatttt gccctgtgat attccgttcg atgagataga
     3241 aaagataaat cccaagggaa taatactctc gggaggacct tcaagtgtat atgaggaaaa
     3301 cgctccaaag tgtagtccgt tgatattcag tgggagatat ccagttttgg gtatctgtta
     3361 tggtatgcag attatggctc atatgcttgg tgggaaggtt ataaagggag ataagggcga
     3421 gtttggcaag acgcttttga aggttgaata taattcagtc ctttttgatg gcttagatag
     3481 agagtttatt gtctggatga gtcataatga tatagttatt aaagcgcccg aaaattttag
     3541 ggttatagct tctacagata atacccctat agctggtata gaagatgatg aaaagaaaat
     3601 ttatggtatt caattccatc ctgaagtgat tcacactata aatggagaca agataataaa
     3661 gaattttctc tacaaggtat gctcctgtag tggtgactgg actcctgaac atttcataaa
     3721 tgataccata gacagtataa ggaaaactgt taataatggt aaggttatat gcgctctaag
     3781 tggtggagta gattctacta ccaccgctgt gcttgtccat aaggctatag gggataatct
     3841 tatctgtata ttcgttaatc agggaactct cagagagaat gaagttgatc aagtactgga
     3901 tacactgaag agtttcaata tgagggtgga gtatatagat gctactgata gattcctggg
     3961 aagattgaat gggataacag accctgaaga gaagcgcaga gtgattggac atgaatttgt
     4021 aaatgtattc gaagaggcag ctgccagaat aggggatata gatttcctcg cccaggggac
     4081 attatatccc gatgtaatag aaagtgctac cagctcccag aaagctcata agataaagac
     4141 ccaccataat gttggtggat tacctgagaa gatgagactg aagctggttg aacctctccg
     4201 taatcttttc aaggacgagg tgaggagagt agctctctct ttaggaatcc ctgaaaagat
     4261 agtctatcga caaccgtttc ctggtccggg gcttgctata agagttatag gagaggtaac
     4321 taaagagaga ctggaaataa ttagaaaggc agatcatata atctctgcag agatagaaaa
     4381 ggctggtctg aataaagaac tctggcagta ttttggtatt cttttgccag agatgagtgt
     4441 gggtgtaatg ggtgacagga ggacatttgg acatgtcgta gctgtaaggg ctatattgag
     4501 tgaggatgga atgactgctg attgggctcg cctaccatat tctcttttag aaacaatttc
     4561 aaacagaata ataaatgaag ttccaaaagt taacagggtg gtgtatgata taagttcgaa
     4621 acctcccgcc actatagagt gggaatagat gtgaggagat atctgaaatg atactaaaac
     4681 aatacataga ggatagagtc ttggatgtcc tgattaaaag tgacattaat aatatcgagg
     4741 tggttgttga taggactcaa aatccagaat ttgggcatta tacaacttct accccgttta
     4801 atctgtctaa gatactgaag aagtcacctt atgatattgc cagagagata gcggagaaaa
     4861 ttcaagaccc cttagccctt atagataaaa tagagataaa gggcggattt ataaatttct
     4921 ttttgaatac tgatgcgttt catcttgtag tggaagatat cctggaaaag aaggagagat
     4981 atggaagatt taatgaggga cagggaaaga aggttcaggt agaatttgta agtgccaatc
     5041 cttccggtcc actccatgtt ggtaatggta gaggaggtgc tttaggagat gtattggcga
     5101 atatttttga atggtttggt tataatgttg agagagaatt ttatgtaaat gatatgggta
     5161 atcagatgaa cctgttagct ctttctgtag aggcaaggat gagggagcta ttaggagagg
     5221 attttgagat cccccaggag ggatatcatg gtgagtatgt taaagatata gccaggtcag
     5281 cccttgaaaa atttggcaat gttatctgga atttatctga agaagagaga ttaaaagaat
     5341 taaaggagtt tgcggaagaa acaattttaa gctggcagag agaggacctt gcgctttttg
     5401 gaataagatt tgattgctgg tatagagaaa gtactctaca taagaataat actcttggag
     5461 aagcccttga gatacttaag agtaagggct atacttatga aagagatggt gctatatggt
     5521 ttgcctctac aagattcgga gatgaaaagg atagggttct tgtaaagagc gatggctctt
     5581 ctacatattt tctggctgat attgcatatc atctggataa gtataagaga ggattttctc
     5641 atataattga tatctggggg gcagaccatt acgggtatgt agccaggatg aaggcttctg
     5701 tagaagcgct gggatatgac ccggatacct tcgagccgat catctatcag atagtgcatc
     5761 tctttcgtgg tggttctgcg gtcaggatgt caaagaggac aggagaaatg gtgtctttaa
     5821 gagaactcct ggatgaagtt ggtaaagatg cggcaagata tttttttgta atgagagatc
     5881 cgaatagtca gctggatttt gatatagaat tggcaaaatc acaatctatt aataatcctg
     5941 tttattatgt acagtatgct tttgccagat gctgcagcct ttttaaacag gcagaattga
     6001 aaggtataga tattggcagg atagaccact gtgatttgtc ctgtttacag gaccccttag
     6061 agagagatat tatgatgatc ctggaggatt ttcctgatat gcttaaaaat gttttttata
     6121 atcgtgctcc ccacagactt accgcctatc tggaatcttt agccaaagga tttcattctt
     6181 tctacaattc tctgcgtata atacaggaaa atgaagattt aatgtatgca cggatggctc
     6241 tggtctctgc caccagacag gtgataaaga atggactgga tatactgggg gtcagtgccc
     6301 ctgaaaagat gtaatatttt tgtgttataa tgaatatagc cttgattttg taggaggtta
     6361 gtaaatggag agtagactgg aaaatccact gggtcaggta agtatatctg ctaatgccat
     6421 agccaatatt gttacaaatg ccactctcag atgtccagga gtggcaggga tgagtaccaa
     6481 gtcttttact gaggagatag ctaatctatt aaaggttgag tctccctcca gaggggtgga
     6541 gataaatata tcagaagata aggttaatgt taatgttttt gttttggtga gttatgggac
     6601 gaatatcccg gagcttgctg accttatagc cagtaatata aagagtgacc tggagaagta
     6661 cacaggattg atagttgctt ctgtaaatgt aaatgtagtg ggtatctctt aagataaagg
     6721 gagtgtaatt gattgtcctt agatatacct gttcagtatg tgaagggagt gggtccgaaa
     6781 cgtgctactt tattcgggag attaggggta gagactttaa gagatttgct gtacttcgcc
     6841 cctaagagat atgaggatag gagtaattta agtcctataa attctttaga gtcagatact
     6901 tatcagacag ttttttgtca ggttgtggat gttaaagaag aaagggtgag gaaaaatctc
     6961 actttaataa aagcaagggt aagagatgat accggagagg ctatcgctat ctggtttaat
     7021 cagacttatc ttaaaaacat ccttaaaaga ggagttaaac tttatcttta cggtaatatc
     7081 tcacggaaat atggcaggat agagatacag aatcccgatt atgaaattgc tgatggtgat
     7141 gagactatcc atacaggtcg tattatacct gtttactcac ttacagaggg tttaagcgag
     7201 aagatagttc ggaacatcat ctggaatact ctgaataact atttggaaga actagaggag
     7261 ttccttcccg aagaaatatt agataaatat ggttttcctg atataaaaag ggctatatgg
     7321 ggtatacatt ttccagatga cgaagttact ctgagaaaat caagggaaag gcttacattt
     7381 gaagaatttt ttatacttca acttatcatt ttaagaagga ggatgtctct tctcaaggag
     7441 tatgctcctg ttatcaaggt ttcgcggaat ttggagaagc gctttttaga aagtcttcca
     7501 tttgagttaa ccaatgcgca gaaaagagta atagaagata ttaagagaga tttggcgagt
     7561 ggaaaatcta tgcagagact tttgcagggt gatgtaggtt caggcaagac agtggttgcg
     7621 gcgttcttcc tct
//
LOCUS       DAOTKU010000014         7612 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_9414_length_7612_cov_3.437211, whole genome shotgun sequence.
ACCESSION   DAOTKU010000014 DAOTKU010000000
VERSION     DAOTKU010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7612
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9414_length_7612_cov_3.437211"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="P5310_00750"
     CDS             <1..270
                     /locus_tag="P5310_00750"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HRU31923.1"
                     /translation="NGEPIVVRGAVKPVPTLMNPLPSVDLITKEETKASVLRSDVCVV
                     PAAGVIGESMLAWVLADTIIDNFGGSTLEEIKIRFNQYCNYVKAM"
     gene            254..769
                     /locus_tag="P5310_00755"
     CDS             254..769
                     /locus_tag="P5310_00755"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HRU31924.1"
                     /translation="MSRLCKPIILIGFMASGKTTIGKELSKILNRDFIDTDELIEKRV
                     GLGIKEIFQRYGEDFFRDTEEKIISEVIKDDNTVIATGGGCILRETTRRLLKEKGLVF
                     WLKIDPETVMNRTDNDNTRPLLLVEKEEKIRTLLAQREHLYRETAHYIIDATEPVESA
                     VSEILTSSGMI"
     gene            838..1884
                     /gene="aroB"
                     /locus_tag="P5310_00760"
     CDS             838..1884
                     /gene="aroB"
                     /locus_tag="P5310_00760"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HRU31925.1"
                     /translation="MKTIKVRLKENSYNIYIGKGILDQFNGLFPSKEPRKALLVTNET
                     VWKLYGKRTVDSINDGFVNVITLILPDGERYKTLRSVEKGYRTLVENKFTRSDCIINL
                     GGGVICDTGGYIAATYMRGIDFYQIPTTLLAQVDASIGGKVAVNLPQGKNLVGTFYQP
                     DGVLIDIDFLNTLPKREFNSGWAEIIKAGILDGENFFSFLERGRYPIISPIEKAIRLK
                     ERVVRIDERDRGLRKILNLGHTFAHAIESATNYRRYLHGEAVSIGMMYAIRLGEAIGV
                     TDETLKDRVKVLLERFELPVELKGIDPQRLIPYFYVDKKATTGSLSFIIPLKIGDVRE
                     FKDVKLEILGGILR"
     gene            1925..2947
                     /gene="aroF"
                     /locus_tag="P5310_00765"
     CDS             1925..2947
                     /gene="aroF"
                     /locus_tag="P5310_00765"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HRU31926.1"
                     /translation="MVIVLTDNATEEDVELICEKVKSLGYGVNVSKGIEKTIIGVIGA
                     KDEQKLILAQQLESLHSVEKVIPILKPYKLASREWKKERTQINVNGVVIGGNDVVVMA
                     GPCSVENEEMIMLTARAIKAANGKILRGGAFKPRTSPYSFQGLGEEGLKVLSQAGKET
                     GLPVVTEVLDPRDVELVSQYTDILQIGTRNMTNYPLLTEVGRTKLPVLLKRGLSSTVE
                     EFLLAAEYILSRGNHRVILCERGIRTFEQSTRFNLDLTAVPLIQELSHLPIIVDPSHG
                     TGRWQLVPSMAKAAIAAGADGLIIEVHPEPDKALSDGAQSLNIPMFQTLMEELTKVAE
                     AVGRSL"
     gene            2944..3756
                     /locus_tag="P5310_00770"
     CDS             2944..3756
                     /locus_tag="P5310_00770"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HRU31927.1"
                     /translation="MRSIGIIGLGIIGGSIALSLKDKGYKIVGMNRSKEPLEKALKSN
                     IIDEAASLDSPPETDITFVCTTVTVTPYFIRKVLDCTDKTIVIDVASTKTFIMKDLDD
                     LPAEKLSRFTGGHPMAGSEKTGIDYAKKDLFVNATFFLTPNRYTSEETISQVKKVVGE
                     LGANPVFISPEEHDKLVAYISHLPQIISTSLSVVVKSAVEDKIVYSGRGYRDMTRLAN
                     SNFSVWRDIIATNRNNISEAIDTYKNFLDRMKSSIDRWDEKELEEIFNMAKY"
     gene            complement(3753..4307)
                     /locus_tag="P5310_00775"
     CDS             complement(3753..4307)
                     /locus_tag="P5310_00775"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012002866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HRU31928.1"
                     /translation="MENPFLVGDKIYFRGLELDDLDKLVSWINDPEVTLFLTMGRIPL
                     NSIREEEWVRNLYKNQNDIVMGIVLKDGDQLIGDIGLHKIDWVSREGTLGIMIGEKEY
                     QNKGYGTEAVLLMLNYAFEVLNLNRIELTVFEFNKRAIRCYEKGGFTLEGRLREKIYK
                     DGMYRDVLIMSILKDEWKDKKGEG"
     gene            4428..5681
                     /locus_tag="P5310_00780"
     CDS             4428..5681
                     /locus_tag="P5310_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRU31929.1"
                     /translation="MFSRIRGNARICLLFETMWVIPYNMFATYSSVYMLSLGLNETQI
                     GLLASTGLFLQIFTSFISGHLTDMLGRRKALLIFDLISWSIATFIWAIAQNFWYFLAA
                     VIVNSFQKIPNTAWYCLLVEDTEPKDRSIIFTILQIISVISGFFAPLGGLLVSKLTLV
                     PAVRIMYLIACISMTIMFFSRNYLTYDTEISIKKKTESKGVKFKESLGEYLDVIKRVI
                     SNRSLKLIFSAYILNNFQMTMRGTYLSIYLVNALKIKDSLIGLFPAFSSAAMLILLFL
                     VVPKFKIEKSSIYMISGFIISMMANVILILLPDGSVSGAIVITMLAAAGNIIAAPYLE
                     ASVANLIEDETRAKTMAILTVLILIFISPAGIIGGWTYSINPKIPFIIISAALLTNAL
                     LIFLFRREEGKERKALEEKLEMVKQ"
     gene            5929..6102
                     /gene="rpmB"
                     /locus_tag="P5310_00785"
     CDS             5929..6102
                     /gene="rpmB"
                     /locus_tag="P5310_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013756658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HRU31930.1"
                     /translation="MAKRCSICGKGPIYGNNVSHSNRRTRRRWSPNLQKVKRNINGEI
                     KRVYVCTDCLKTI"
     gene            6103..7302
                     /gene="ilvA"
                     /locus_tag="P5310_00790"
     CDS             6103..7302
                     /gene="ilvA"
                     /locus_tag="P5310_00790"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006549 - isoleucine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HRU31931.1"
                     /translation="MTPLEKSREARALLTQIIHKTPLQYSTTLSDWVSQNAYLKCENF
                     QKTGSFKVRGAYFKLSQLSKEEKERGVIACSAGNHAQGVAYASSKLGIDALIVMPEFA
                     SISKIEATKGYGGKVFLYGRTFDESHEKALEIAGETNRVFISPYDDPDIIAGQGTLAL
                     EILEDLPEVDMIVSPVGGGGLISGIASVIKSVNPKVKVIGVQAKAVPSVYNMMKRREE
                     EPVTCETIADGIAVKKPGKLTLEIISKLVDDIVLVEEEEIAGAILFLLERCKMIAEGA
                     GAVGVSAIMYNKIPLGRNTVAVVSGGNIDVNLISTIIERGLTKSGRRLKFITTIPDRP
                     GELYKLLTVIARIKANIISIHHERLNPVVPIGLVEVEISVETRSKEHTEELIEAIGSS
                     GYRVKII"
     gene            complement(7336..>7612)
                     /locus_tag="P5310_00795"
     CDS             complement(7336..>7612)
                     /locus_tag="P5310_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU31932.1"
                     /translation="RLGLPSNCPFKLWLNGEKIHEAVTEGILRPNYGGDGRSYVVSDL
                     KEGWNQILVKIERKGKPVEAHFTVASGDRFSHGLADLVECKFPWEMI"
BASE COUNT         2559 a         1299 c         1635 g         2119 t
ORIGIN      
        1 aatggagagc ccatcgtagt aagaggagca gtaaaaccag ttccaactct gatgaacccc
       61 ctaccaagcg tagatttaat tacaaaagaa gagacaaaag catcagtctt aagaagtgat
      121 gtatgtgtgg tccctgctgc tggagtaatt ggagaatcaa tgctggcatg ggtattagca
      181 gatacaatca tagataactt tggtggaagt acactggaag aaataaaaat caggtttaac
      241 cagtactgta attatgtcaa ggctatgtaa acccataatt ctaatcggtt ttatggcttc
      301 cgggaagacg actataggga aagaactttc aaaaatttta aatagagatt ttatagacac
      361 agatgaactc atagaaaaaa gggtgggatt aggaataaaa gaaatctttc aaagatatgg
      421 ggaagacttt ttcagagata cggaagaaaa gattatctca gaagttataa aggatgataa
      481 tactgtaata gcaactggtg gtgggtgtat tctacgtgaa acaaccagaa ggcttctcaa
      541 ggagaaaggg ctggtattct ggctaaagat tgaccctgag actgttatga atcgaacaga
      601 taatgataat acaagaccat tactgctggt agaaaaagag gaaaagataa gaacacttct
      661 tgctcagaga gaacatcttt acagagaaac agcccattac ataatagatg ccacagaacc
      721 cgtagagagt gctgtctcag agattctaac atcatcaggc atgatataaa tcggtattac
      781 attattcgga atcaaattgc tatatctata atagaaaaat ataatgttct ctaacctatg
      841 aagacgataa aggtaagatt aaaagagaat agctataata tctatattgg aaagggtata
      901 ctagatcagt ttaatggtct tttcccatct aaagagccca gaaaagccct gctcgtaact
      961 aatgagactg tctggaaact ctatggaaaa agaacggtgg actctataaa tgacggtttt
     1021 gtaaatgtca ttacactgat actgcctgat ggggaaagat acaagacttt gagatcagta
     1081 gaaaaaggtt ataggactct ggtcgagaat aaatttaccc gaagtgattg tattataaat
     1141 ctgggtggtg gagtaatctg tgataccggt ggttatattg ctgcaacata tatgcgaggt
     1201 atagacttct atcagatacc aaccacactc ctggctcagg tagacgcaag tataggaggt
     1261 aaggttgcgg ttaacctgcc ccaggggaaa aacctcgttg gaacattcta tcaaccagat
     1321 ggagtactga tcgatataga ttttctgaac acactaccaa agagagagtt taactctgga
     1381 tgggctgaga ttataaaggc tgggattctg gatggagaga atttcttttc ctttttggaa
     1441 aggggaagat atccaataat ctcccccata gaaaaagcca taagacttaa ggaaagagtt
     1501 gtaaggatag atgaaaggga cagaggatta agaaaaatac tgaatcttgg gcatacattt
     1561 gcccatgcaa tagaatcagc tacaaattat agaaggtatc tccatggaga agcggtatcc
     1621 ataggaatga tgtatgctat aaggcttggt gaagcaatag gagtaactga tgagacgtta
     1681 aaagataggg taaaagttct cctggagaga tttgaattac ccgtagaact taaggggata
     1741 gatccgcaga gattgatacc ctatttctac gttgataaaa aggctactac agggtctcta
     1801 tcatttatta tccctttaaa gataggggat gtaagagaat ttaaagatgt aaaattagag
     1861 atattaggag gtatattaag atgattgaac tccgggactt tacgaaagta taagggaggc
     1921 aaatatggta atagtactta ctgataatgc cacagaagaa gatgtagagt taatatgtga
     1981 aaaggttaaa agtttgggtt acggggtaaa tgtatcaaaa ggtatagaaa aaactattat
     2041 aggagttatt ggggctaagg atgaacagaa gttaatcctg gcacagcagt tggaatcact
     2101 ccacagtgta gaaaaagtca tacccatact gaagccttat aaactggcaa gtagagagtg
     2161 gaaaaaggaa agaacgcaaa taaatgtaaa cggggtggta atcggtggaa atgacgtggt
     2221 tgttatggcg ggaccatgtt cagtagagaa tgaagagatg attatgctta ccgccagagc
     2281 aataaaagcg gcgaacggca agatcctgag aggaggtgca tttaaaccga gaacatctcc
     2341 ctatagtttt cagggattgg gagaggaagg actcaaagta ctctctcagg cggggaaaga
     2401 gacagggtta cctgtagtta ccgaagtact cgacccgaga gatgtagaac ttgtaagtca
     2461 gtatactgat attctccaga taggaacacg taatatgaca aattatcctt tactaactga
     2521 agttggtcgt acaaaattac cggtactttt aaaacgtgga ttaagttcta ctgtggagga
     2581 atttttattg gcagcagagt atatactgtc cagaggaaat cacagggtaa tcctctgtga
     2641 gagaggaatc agaacatttg aacaatccac cagatttaac ctggatttaa cagctgtccc
     2701 tcttattcaa gaactcagcc atctacctat tatagtagac ccaagccatg gaactgggag
     2761 atggcaactt gttccctcta tggctaaagc agccatagct gccggggcag atggattaat
     2821 aatagaagtc catccagagc cagataaagc cctatctgat ggggctcaat ctttaaacat
     2881 acctatgttt cagacattga tggaagaact gactaaggtg gcagaggctg taggaagaag
     2941 cttatgaggt cgataggtat aataggtcta ggtataatag gtggttctat tgctctttct
     3001 ctaaaggata aaggatataa aatagtaggt atgaacaggt caaaagaacc actggagaag
     3061 gctttaaaat caaatattat agatgaagct gcgtccctag attctcctcc ggaaacagat
     3121 attacttttg tgtgtacaac tgtaacagtt accccttatt tcataagaaa agtcctggat
     3181 tgtaccgata aaactatagt tatagatgtg gccagtacca agacttttat aatgaaggat
     3241 ctggatgacc tgccggcgga gaaattgtcc cgttttacag gtggtcatcc aatggctggt
     3301 tcagagaaga ccggtataga ttatgcaaaa aaagacctat ttgtcaacgc aacattcttt
     3361 ctaacaccaa atagatacac ttcagaagaa acgatatctc aggtaaagaa ggtggtagga
     3421 gagcttggag ctaatcctgt atttatatcc ccagaagagc atgataaact tgtagcttat
     3481 ataagtcatc tacctcagat aatatccaca agcctcagtg tcgtggtaaa atcagcggta
     3541 gaggataaga tagtctattc agggagaggg tatagggata tgactaggct tgcaaatagc
     3601 aatttctcag tctggagaga tatcatagcc acaaacagaa ataacatatc ggaagctata
     3661 gatacttata aaaatttcct tgacaggatg aagtccagca tagatagatg ggatgagaaa
     3721 gagttagaag agattttcaa tatggctaag tattaacctt cccctttctt atctttccat
     3781 tcatctttta agatactcat tataagcaca tctctataca tgccatcttt atatatcttc
     3841 tctcttaatc ttccctctaa agtaaatcct cccttttcat aacatctgat ggctctttta
     3901 ttaaattcaa agaccgtcag ctctatcctg ttgagattca gaacttcaaa tgcataattc
     3961 aacattaaga gtactgcttc tgttccgtaa cctttattct gatattcttt ttctcctatc
     4021 attattccca aagtcccctc tctggaaacc caatctatct tatgcagacc tatatcacct
     4081 ataagctggt ctccatcctt caagactatc cccataacta tatcattctg gttcttatag
     4141 agattcctga cccactcctc ttctctgata ctgttcaggg gaattcttcc cattgttaaa
     4201 aacaaagtaa cctccggatc atttatccag ctgacaagtt tatccaggtc atctaactct
     4261 aatcctctaa agtatatctt gtctccaact aaaaagggat tttccataaa ggaccacctt
     4321 ctttttagat gagaatatag cacgtaggat ctgaaaaatc aaatgaagat tgccttttac
     4381 tctttattat ggtaagcttt tctaaaaaag gaaggagaaa gggacgattg tttagcagaa
     4441 tcaggggaaa cgccaggata tgtcttttat ttgagaccat gtgggttatt ccttataata
     4501 tgttcgccac ttattcatcg gtctatatgt tgtccctggg acttaatgaa actcagatag
     4561 gacttcttgc atctacaggt ttgtttcttc agatcttcac ctcatttata agtggacatc
     4621 ttacagatat gcttgggagg aggaaggctt tattaatatt tgatctgata agctggagta
     4681 tagccacttt catatgggca atagcacaga atttctggta tttccttgca gcagttatag
     4741 tgaatagctt tcaaaagata ccaaacaccg cctggtattg tcttttagta gaggataccg
     4801 aaccaaaaga taggtctatc atctttacca tacttcagat tatctcagta atatcaggct
     4861 tctttgctcc attgggagga ctacttgtaa gtaaactcac actggtacct gcggttagaa
     4921 tcatgtatct cattgcctgt ataagcatga ccattatgtt ctttagcaga aattatctta
     4981 cttatgacac agaaataagt atcaaaaaga agacagagag taaaggggta aaatttaaag
     5041 agagcttagg agaatatctg gatgttataa aaagagtaat ctccaataga tcattaaaat
     5101 taatattcag cgcctatatc ttaaataatt ttcagatgac tatgagaggg acatacctgt
     5161 caatatatct ggtaaatgcc ctcaaaataa aagattcact gatagggttg tttccagcct
     5221 tttcttcggc agctatgctc atactgttgt ttttagtagt tcctaaattt aaaattgaga
     5281 agtccagcat atatatgata tcaggtttta tcatttcaat gatggcaaac gtgatactga
     5341 tcttacttcc agacggaagt gtatcagggg ctatagttat tacaatgctt gcagcagccg
     5401 ggaatataat agctgcccca tatcttgagg catctgtggc taacttgata gaagacgaga
     5461 cgagagccaa aactatggca atcttaacgg tattgattct tatattcata tccccagcgg
     5521 gtattatagg aggctggact tacagtataa atccaaagat accatttata attatctcag
     5581 cagcacttct gactaacgca ttattaattt ttttatttag aagagaggaa ggaaaagaga
     5641 ggaaagcgct agaagagaaa ctagagatgg tcaaacaata aaacaataag gaccctttcg
     5701 ggtccttatt gtaagggggg taggtcatat gatgaggtta ccttaactgc taaatcatta
     5761 atatcaactt cattaactat aaggtagtat agggggaata tattaagaaa ttattaagat
     5821 aagattaaga gtttattaag attatgtcta ttgactttag ggtataatga atgttaacct
     5881 tattagtgat gatgcccttg cctatttaaa gttaaggagg gaaaaattat ggcaaaaaga
     5941 tgcagtatat gtgggaaagg acccatttat ggaaataatg ttagtcattc aaacaggaga
     6001 acacgtagac gctggtcacc taacctgcag aaggtgaaga gaaatataaa cggagagatt
     6061 aaaagggttt acgtctgcac agactgtcta aaaacaatat aaatgactcc acttgaaaaa
     6121 agtagagagg caagggcact cctaactcag ataatacata agacaccact tcaatactct
     6181 actacccttt ctgactgggt atcgcagaat gcatatctga aatgcgagaa cttccagaag
     6241 acaggctctt ttaaagtgag aggagcatat tttaagcttt cacagttatc taaagaggaa
     6301 aaggaaagag gggttattgc ctgctcagca gggaatcatg cccagggagt agcatatgcc
     6361 agctccaagc tggggataga cgctctcata gttatgccag aatttgcatc tataagtaaa
     6421 atagaagcga caaaggggta tggaggcaag gtattcctgt atggaagaac gtttgacgaa
     6481 tcgcatgaga aagccctgga aatagcaggg gaaacaaaca gagtatttat atcaccctat
     6541 gatgatcctg atattatagc cggacaggga accttagccc tcgagatttt ggaagactta
     6601 cctgaggtgg atatgatagt atcaccagtt ggtggaggag gacttatatc tggtatagcc
     6661 tctgtaataa agagtgtaaa ccctaaggtt aaagtaattg gagtccaggc aaaggcagtc
     6721 cccagcgtat ataatatgat gaagaggcga gaggaggagc ctgttacctg tgagactata
     6781 gcagatggga tagctgttaa aaaaccggga aagctcacgc tggaaatcat ttcgaaatta
     6841 gtagacgata ttgttcttgt agaagaggaa gagatagcag gggctatttt gtttctgttg
     6901 gaaaggtgca agatgatagc agagggagct ggagcagtag gggtttctgc cataatgtat
     6961 aacaagattc ctttaggtag aaatacagtg gcggtggtaa gtggtggcaa tatagatgta
     7021 aatctcatct ctactataat tgaacgcgga ttaaccaaat cgggaagaag actaaaattc
     7081 ataacaacca tacctgatag acccggagaa ctctataagt tgctgacagt aattgccagg
     7141 ataaaggcta atattatctc aatccatcac gaaagattaa atcctgtggt tcctatagga
     7201 ttagtcgagg tagaaatatc tgtagaaact agaagtaaag aacatacgga agagcttata
     7261 gaagccatag ggtcttcagg ttacagggta aaaataatat aaagggcaga ggattttctc
     7321 tgccctttat tatctttata tcatctccca gggaaatttg cactctacaa ggtcagctag
     7381 accatgtgaa aatctatctc cagatgcaac tgtgaagtgt gcttctacag gttttccctt
     7441 cctctctatc ttaactaaaa tctgattcca tccctctttt aaatctgaaa ccacatagct
     7501 tcttccatca ccaccatagt ttggtctgag tatcccttct gttaccgctt cgtgtatctt
     7561 ttctccatta agccataact taaatggaca attggaaggt aatcctaacc tg
//
LOCUS       DAOTKU010000015         7600 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_9431_length_7600_cov_3.244003, whole genome shotgun sequence.
ACCESSION   DAOTKU010000015 DAOTKU010000000
VERSION     DAOTKU010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7600
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9431_length_7600_cov_3.244003"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            376..1659
                     /locus_tag="P5310_00800"
     CDS             376..1659
                     /locus_tag="P5310_00800"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRU31933.1"
                     /translation="MSKRFLTVILLLTFIASMFSVTTAYAKVKITYWTGWGGSELEDL
                     KKIIDEGFNKKRTDIEVETVTIFGAYEKLLTAIAGGTVPDVVSAVWDSQLASLAARDA
                     LTPLDSYIKKDKIRSSDFWPRLWQSFQYDGKVYALAATTNCQFTAFNVDMVQAAGLNP
                     KTPPKNTKELYIWAEKLTKFDDRGNIQILGYRPSGISLWGRLFGGKLYDEKNKKVTAN
                     DPKVVAAMEYLLSYNKFLDPTKYTAFTSALGNYWSPENPFFAGKMAMQDYGEWIVQFG
                     KKYNPKLKYDIFPYVTPSGTRNSTQFGGSMFCIPTGSKNKDAAWEFIKWLTSPEPMKK
                     LAIIFTNMPPRIAVAKDPEVLNAIPVLKLTIPLMEKGNAFTSVSMPVWEQYLTELGAA
                     ESMVINGQKKPKEALDEVTLRMQQELDTFFSRQKR"
     gene            1719..2609
                     /locus_tag="P5310_00805"
     CDS             1719..2609
                     /locus_tag="P5310_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRU31934.1"
                     /translation="MTKLEKKELMWGLFFTFPWLLGLIIFTAYPIIMSLYYSFTDYNI
                     VQEPLWIGLDNYRRLIRLDPIFWKSLGNTFYMLCLGLPLSLIAGLLLALLLNTNVKGM
                     AIHRTIFYLPSILPEVATAILWVWLFNPYYGLVNVILSWFGIQGPGWLADPAWAKPAI
                     ILMGLWGVGGGMIIYLASLQDVPKELYEAAELDGARGWQKMVNITLPMISPVIFFNLI
                     MGIIGTFQFSVQTFVATGGGPAYSTMFYGLYLYYNAFQWWKMGYASAMAWILFLITLA
                     LTILVFKTSARWVYYHSESR"
     gene            2621..3478
                     /locus_tag="P5310_00810"
     CDS             2621..3478
                     /locus_tag="P5310_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRU31935.1"
                     /translation="MKENWRKTLRRRAIIKEVGLQALLILLGFTFLLPFLWLISTSLK
                     SNEQIFTSPPVWIPNPVVWSNYKKALEYFPFFRFLKNTLYITAFNVIAAILAPSFVAY
                     GFARIKWPGRDILFLILLSTMMIPSQVTMIPVFVMFKKIGWVGTFKPLIVPAFFGGGA
                     FSIFLMRQFFMTIPQELVDAAKVDGCSHPYIYWNIMLPLSKPAIATLGVFAFIGSWRD
                     FLGPLIYLQDERQYTLAIGLRYFLTQHGAEWAYLMAASTVVSIPMIVAFFLAQKTFIQ
                     GITFTGIKG"
     gene            3484..4533
                     /locus_tag="P5310_00815"
     CDS             3484..4533
                     /locus_tag="P5310_00815"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRU31936.1"
                     /translation="MDIDFSSHNREVKEVWDAYNSGKPVRVPVYFNTNPRMILLDPEL
                     NEEGVTFKDYFESPEVMAKVQMKFRKWVRFNVPQDREMGYPENDWGGCWVDFQNSYEA
                     LYFGCKLFYWDDSLPDIEPVLKDNKEFLKDINPEPEDNPFLKQIMDYYDYFLSISKDL
                     FEGFPVGKPHIPLLGTDGPFTVAAQIRGASQLCIDIYEDPEFVHNLLSFITDATIKRI
                     TYMMDRFGIKHPTQSWGFADDSIELLSEKTYREFVLPYHKKLFNTFSLGGPNSIHLCG
                     KASHHFLTIKEELNVMDFDTGYPTDLGKMRKVLGPEVTLRGNISPMILREGPVEEIRG
                     EVIKVLQSGVMEGGR"
     gene            4647..5735
                     /locus_tag="P5310_00820"
     CDS             4647..5735
                     /locus_tag="P5310_00820"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRU31937.1"
                     /translation="MKAKERVRRALERKEPDRVPVFPVITALHAARATGIRYRDVVLN
                     PWLNYEALLRAWRLYGFDGFEVGLASRLDKDISIVKKSGEEFLSKNGKLLARLQEHDM
                     PIQLENEIPIKDEKDIEKIRIKTWKEYIQDGQAEPVKKVLEEVGEDAFIAGTAASQSM
                     NFLVSMRGSQQALLDLIDNPELAHKIMELGTEISIQIGYALIEAGVDAIYIGDAWASS
                     TIISPGQYKEFCFPYHKKATEAFHKKGTPVYLHICGNSMPILEYMAETGVDAIEPLDP
                     LGGVDIAEVKRRVGNKVCLKGGVNTLTLLRGTPEDVMEEAKHCIESAGRGGGYILGSG
                     DDIPTDAPVENVKAMVEAAKIYGRYIGG"
     gene            5736..7247
                     /locus_tag="P5310_00825"
     CDS             5736..7247
                     /locus_tag="P5310_00825"
                     /inference="COORDINATES: protein motif:HMM:NF024941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4127 family protein"
                     /protein_id="HRU31938.1"
                     /translation="MNIGFLPLDERPCNFKFPQYIARIAGVELILPPKEILSRKRTPA
                     DLKAIDEWLERESDNFDALVLSIDMFTYGGLIASRISQASTEESVERLEKIRDIKLKH
                     RDLPIYAFNIIMRTSNSNNNEEEKEYWKDYGMLLFQYSELTHKVKLFNREEDKQLLHE
                     VERNIPEEIIKDYLNGRERNHKVNLALIDLVKENIVDFALLTQDDASPYGFPAMEQKE
                     LRAKIREGRVQSKVIIYPGADEVGMLLIARAVNVKEGVKPIFKADFSSVNGPHIITRY
                     EDRPLFEGIKGQINAVGGVLVDSISLAGIELLVNTPAIEQGEAYLFYNMDKVEGPARN
                     LLSLEQIGRYCTEVLNMPVVLADVAYANGADLELIELLEETGFVENLTCYAGWNTSGN
                     TLGTVIAAGSAWISGSEIEDCEMALREFREVRLIDDWLYQAKVRTDVIEELKSRGINP
                     WQLEDVEMARIMVEEKLKEEVEKRSEEIFPLKIKRIDLPWNRLFEIDIHFEEK"
     gene            7248..>7600
                     /locus_tag="P5310_00830"
     CDS             7248..>7600
                     /locus_tag="P5310_00830"
                     /inference="COORDINATES: protein motif:HMM:NF016054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HRU31939.1"
                     /translation="MESIIERLRDGVIVSCQAYPGDAMYGSNIMAAYAKASFESGAAG
                     IRANGVEDIRAIKKAVPVPVIGIYKDTIPGYEPYITPTLRHVEMVISAGADIVAVDAT
                     DRLHPEGVTGNRYIE"
BASE COUNT         2381 a         1200 c         1795 g         2224 t
ORIGIN      
        1 gcgagattat tataaaagac gaaattgtag gggcaattca tgaattgccc ctacgtgata
       61 ctttgcatct agcgtctttc ttcaaatact ttcaaatatt atttgagact gtactttctg
      121 acttttcttt ataccctttt gagagataat tcattttgtg aaagattatt tttgaagaac
      181 atcttattta gaatccttgt ggaataaaat tcaaaattct attgacatat atagaaattt
      241 atgctataaa ttaaccgaat atattaagga aagagggtga gaaagaagag gctttagata
      301 ttttgtgtgc agtacttgaa gggggatgat gagatctaaa gatttgtatc aaaaactaat
      361 taggaggtgt accaaatgag taaaaggttt ttaacggtaa ttttactttt aacatttata
      421 gcaagtatgt tttcagttac tacggcttat gctaaggtaa aaatcaccta ctggacaggt
      481 tggggaggtt cagaattaga agatcttaaa aagatcatag atgaggggtt taacaaaaag
      541 cggacagata tagaggtaga aacagtaaca atctttgggg cttatgaaaa gcttcttact
      601 gctatagctg gtggaacagt tccagatgta gtttcagctg tttgggattc tcagttagcc
      661 tctttggctg ccagagatgc tctcactcct cttgatagtt atatcaaaaa agataagatc
      721 cgtagtagtg atttctggcc aagactctgg caatctttcc aatatgatgg taaggtttat
      781 gccctggcag caactaccaa ttgccagttt actgctttta atgtagatat ggtccaggct
      841 gccggcttga atcctaaaac tccaccaaag aatactaagg aactttacat ttgggcagag
      901 aaactcacaa agttcgatga tagaggtaat atacagatcc ttggttatag accaagtggt
      961 atatctctat ggggaagact ctttggtggt aagttatatg acgaaaagaa taagaaggta
     1021 acggctaatg accccaaggt agtagcagcc atggagtatc tcctttctta taataaattc
     1081 ctggacccca caaagtatac agctttcact tcagcattgg gtaactactg gagcccagaa
     1141 aacccgttct ttgcaggaaa gatggcaatg caggattatg gtgaatggat cgtgcaattt
     1201 ggtaagaaat ataatccaaa gctcaaatac gatatattcc catatgtgac tccaagcgga
     1261 accagaaatt ccactcagtt tggcggcagt atgttctgta ttccgacagg atctaaaaat
     1321 aaagatgcag catgggaatt tatcaagtgg cttacaagcc cggagccgat gaaaaaactt
     1381 gcgataattt ttactaatat gcctcctaga atcgcagtag caaaagaccc agaagtctta
     1441 aatgccattc ctgtattaaa acttacgatt cctttaatgg aaaagggtaa cgcatttacc
     1501 tctgtttcca tgcctgtatg ggaacagtat cttacagaac ttggcgctgc agaaagtatg
     1561 gtaattaatg gtcaaaagaa accaaaggaa gcacttgatg aggtcacgtt aaggatgcaa
     1621 caagaactgg atactttttt ctctaggcaa aagaggtaag ctctagttat tagagagggg
     1681 agaagataat ttccccctct ctagatttgg aggataagat gacgaagtta gaaaagaaag
     1741 aactgatgtg gggattattt tttacttttc cgtggctctt aggtcttata atatttactg
     1801 cttatcctat aatcatgtct ctttattata gttttaccga ttataatatc gttcaagagc
     1861 ctttatggat aggtcttgat aattatagaa ggttaattcg cttagaccct atcttctgga
     1921 aatcattggg taatactttt tatatgctct gtttagggtt acctttaagt ctaattgctg
     1981 gattattatt ggccctgctc ttaaacacca atgttaaagg gatggctata cacaggacaa
     2041 tattttacct gccttccatc ttgccagaag ttgctaccgc catactttgg gtatggcttt
     2101 ttaatccata ttatggattg gttaatgtca tattatcgtg gtttgggata caaggtccag
     2161 gatggttggc agatcctgca tgggctaaac cggctattat tcttatggga ttatgggggg
     2221 taggaggagg aatgataata tatctggcta gtcttcagga tgttccaaag gaactttatg
     2281 aggctgctga attggatggg gcaagaggat ggcagaagat ggtaaatatc actttaccta
     2341 tgataagtcc tgtaatattt tttaatctta ttatgggtat tatagggaca ttccagtttt
     2401 cagtccagac gtttgttgct actggtggtg gtccagcata ttctaccatg ttttatggac
     2461 tttatcttta ctataatgcc tttcaatggt ggaagatggg atatgcttcg gcaatggctt
     2521 ggattctatt tttaataact ttagctttaa ccattcttgt atttaagaca tctgcaagat
     2581 gggtatatta tcattcagag tctagatgat ggaggagaag atgaaggaaa actggagaaa
     2641 aaccttaaga agaagagcga taataaaaga agttggtctt caggctttat tgattcttct
     2701 gggattcaca tttttactgc catttttatg gcttataagc acatctttaa aatctaacga
     2761 acagattttt acttctcctc cggtatggat accaaatcca gtagtatggt ctaactataa
     2821 gaaagcatta gagtattttc cattctttag atttctcaag aatacattgt atataaccgc
     2881 ttttaatgtg attgctgcaa tattagctcc ttcttttgtt gcttatggat ttgccaggat
     2941 aaaatggcca gggcgtgata ttttattcct catcctgctt agcactatga tgattccaag
     3001 ccaggtaact atgattccag tatttgtgat gtttaaaaag ataggctggg ttggaacatt
     3061 taaaccgctt attgtccctg cattttttgg tggaggggca ttctctatat tcttgatgcg
     3121 tcaattcttt atgactatac ctcaagaatt agttgatgct gcaaaggtag atgggtgttc
     3181 tcatccatac atatactgga atataatgct cccattatct aagccggcta tagccacttt
     3241 gggagtattt gcatttattg gtagctggag agattttctt ggtcccctta tatatctaca
     3301 ggatgagaga cagtatacat tagctatagg gctaagatac tttctgacac agcatggagc
     3361 agagtgggct tatctaatgg cagcatctac agttgtttct atacctatga tagtggcatt
     3421 tttcttggca caaaagacat ttattcaggg tataacattt accgggataa aaggatgagc
     3481 gatatggata tcgatttttc atctcataat agagaagtta aagaggtatg ggacgcatat
     3541 aattcaggta agcctgtaag ggtacctgta tacttcaata caaatcccag gatgattctt
     3601 ttagaccctg aattaaatga agaaggagtt acatttaaag attattttga atctcctgag
     3661 gtgatggcaa aggtacagat gaaatttaga aaatgggtga ggtttaatgt ccctcaggat
     3721 cgagagatgg ggtatccaga gaatgattgg ggaggatgct gggtagattt ccagaattct
     3781 tatgaggcat tgtactttgg ctgcaagctc ttctactggg atgattctct ccctgacata
     3841 gaacctgtat taaaggataa taaagaattc cttaaagata ttaaccctga gccagaggat
     3901 aatcctttcc ttaagcagat tatggattac tatgattatt ttctgagtat ttctaaagat
     3961 ttgtttgaag gatttcctgt gggaaagccg cacattccac ttttaggaac agacggtcct
     4021 tttactgtag ctgcccagat aagaggtgcg agtcaactct gtattgatat ctatgaagat
     4081 ccagagtttg tccataacct gctctctttt ataacagatg ctactataaa aaggattact
     4141 tacatgatgg atagattcgg tataaaacat cctactcaat cctggggttt tgctgacgat
     4201 agtattgaac ttctctctga gaaaacttat agagaatttg tccttcctta tcataaaaag
     4261 ctgttcaata ccttctccct tggtggtcca aattcaatac atctctgtgg taaggcatcg
     4321 catcattttc ttacaataaa agaggagttg aatgtgatgg attttgatac cggatatccc
     4381 acagatctgg gcaagatgag gaaagtcctt ggtcctgaag tgacactcag ggggaatata
     4441 agtccgatga tactcaggga aggtcctgta gaagagataa gaggagaagt gataaaagtt
     4501 ttgcagtccg gagttatgga agggggaaga tagctcctct gtgagggtaa caatgtagcc
     4561 ccctgcacac cgatagaaca tatgaggacg atgtatgaga cagggaaaga atatggtaga
     4621 tatagataaa ctagtggaga gttaatatga aggcaaaaga aagagtgaga agggcgttag
     4681 agagaaagga acctgacagg gttcctgttt tcccggtaat taccgctcta catgcagcta
     4741 gagccactgg aataagatac agagatgtag ttttgaaccc ctggttaaac tatgaggcat
     4801 tgttgagggc atggaggctg tatgggttcg acggtttcga ggtaggatta gcttctaggc
     4861 tggataaaga tatctccata gtgaagaaaa gtggcgaaga gtttctgagt aagaatggga
     4921 aattactggc gagacttcag gagcatgata tgccaatcca gcttgaaaat gagattccaa
     4981 tcaaagatga gaaggatata gaaaagatca ggataaagac atggaaggag tatattcagg
     5041 atggacaggc agaaccggtg aagaaggttc tcgaagaggt aggagaagat gcctttatag
     5101 ctggaacagc cgccagccaa tctatgaatt tcctcgtttc tatgagaggt tcacaacagg
     5161 cactgctaga cctgatagat aatccagaat tagctcataa gattatggaa ttgggaacag
     5221 agatttccat acagataggt tatgccctta tagaagcagg ggtagatgcc atttatatag
     5281 gagatgcatg ggcgtcctct accattatct caccaggaca gtataaagaa ttctgttttc
     5341 catatcataa aaaagctacc gaagctttcc ataaaaaggg aactcctgtt tatctccata
     5401 tatgtggaaa ctctatgcct atcctggagt atatggctga aacaggagta gatgctattg
     5461 aaccgttgga ccctttagga ggggtagata ttgctgaagt aaagagaaga gttgggaata
     5521 aggtatgcct taaaggtggg gtaaatactc ttactcttct tagaggaact ccggaagatg
     5581 tgatggaaga ggctaaacat tgtatagaat cagctgggag aggtgggggt tatatcctgg
     5641 gttcaggaga tgatattccc acagatgctc cggtggagaa tgtaaaagcg atggtagaag
     5701 cagctaaaat ttatggaaga tatataggag gctaaatgaa tattggattt ttacctctag
     5761 atgaaagacc gtgtaacttt aaattccctc aatatatagc cagaatagct ggagtagagc
     5821 taattcttcc accaaaggag attttaagca ggaagagaac tccagcagac ctgaaggcga
     5881 tagatgagtg gctagaaaga gagtctgata actttgatgc tctggtatta tccattgata
     5941 tgttcaccta tggggggctt atagcatcga ggatttcaca ggcttctact gaagaatctg
     6001 tagagagact ggagaagata agagatataa aattaaaaca cagagatctc cccatatatg
     6061 catttaacat tattatgagg acatccaatt ctaacaataa cgaagaggag aaggaatact
     6121 ggaaggatta tgggatgtta ctgtttcagt actcagagct cacccataag gttaaattat
     6181 tcaatagaga agaagataaa caattactgc acgaagttga gagaaatatt ccagaagaga
     6241 ttattaagga ttatctgaat ggaagagaga gaaaccataa agtaaatctt gctcttatag
     6301 accttgtgaa agaaaatata gttgactttg cccttcttac ccaggatgat gcgagtcctt
     6361 atggttttcc agcaatggag caaaaagaac tgagggctaa gataagagag ggaagagttc
     6421 agagtaaggt aattatatat cctggagccg atgaagtggg gatgcttctt atagcaaggg
     6481 cggtaaatgt taaagagggt gttaagccta tctttaaagc tgatttcagt tcggtaaatg
     6541 gaccgcatat aattacaaga tatgaagata gacctctttt tgagggtata aagggacaga
     6601 taaatgctgt agggggtgtc ctggttgatt ctataagttt ggcaggtata gagttgcttg
     6661 taaacacccc tgccatagag cagggagagg catatctatt ctataatatg gacaaagtgg
     6721 aaggaccggc aaggaacctc ctctctcttg aacagattgg gaggtattgc actgaagtat
     6781 taaatatgcc ggtggtttta gctgatgtgg cgtatgcgaa tggggcagat ttagaactga
     6841 tagaacttct ggaagaaaca ggatttgtag aaaaccttac ctgttacgct ggctggaata
     6901 catcggggaa tacactgggg acagtgatag cagccggttc ggcatggatt tctggatctg
     6961 aaattgaaga ttgcgagatg gctttaagag agtttcgtga ggtaagactt atagatgact
     7021 ggctatatca agcaaaggta aggacagatg ttatagaaga gcttaaaagt cggggtataa
     7081 atccgtggca gttggaggat gtggaaatgg caagaataat ggtagaggag aagttgaagg
     7141 aagaggtaga gaagaggtct gaagagattt tcccgcttaa gattaagagg atagacctgc
     7201 cgtggaacag gttattcgag atagatatcc attttgagga gaaatagatg gaaagtatta
     7261 tagaaagact cagagacggg gttatagtct cctgtcaggc atatccaggt gatgcaatgt
     7321 atggctctaa cataatggct gcttatgcaa aagcaagctt cgagtctgga gcggcaggta
     7381 taagggctaa tggggtggag gatatccggg caataaagaa ggctgtccct gtacctgtaa
     7441 taggaatata taaagatact atccccggtt atgaaccata tattactcca acactgaggc
     7501 atgtggagat ggttatttct gcaggagcag atatagtggc tgtagatgca actgacaggc
     7561 ttcatccaga gggagttaca ggtaacaggt atatagaaga
//
LOCUS       DAOTKU010000016         7523 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_9544_length_7523_cov_3.482994, whole genome shotgun sequence.
ACCESSION   DAOTKU010000016 DAOTKU010000000
VERSION     DAOTKU010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7523
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9544_length_7523_cov_3.482994"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /gene="plsX"
                     /locus_tag="P5310_00835"
     CDS             complement(<1..674)
                     /gene="plsX"
                     /locus_tag="P5310_00835"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HRU31940.1"
                     /translation="MRIALDAMGGDFAPQVLVEGAYIAKDNLDVELVLVGDEAKIREI
                     AGNRELPFEIIHTEEAIQMNEHPAQAVKSKKRASLVLAVEMLRDKQVDAVVSAGNTGA
                     FVACASLILDTISGIERPSIGITIPTLNHPVFIIDTGANIDCKPVYLYQFAIMGMSYS
                     HSILGIDNPRVGLINIGSEETKGNSLTKSTYTILKSIPNFVGNIEGNDIFENKADVVV
                     CDGFIG"
     gene            complement(671..856)
                     /gene="rpmF"
                     /locus_tag="P5310_00840"
     CDS             complement(671..856)
                     /gene="rpmF"
                     /locus_tag="P5310_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HRU31941.1"
                     /translation="MANPKKKMSRCRTHRRRSLWKMSPPSVIRCPQCHEWTLPHRVCP
                     SCGYYDGEVVLTIKEEK"
     assembly_gap    1048..1057
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1079..1411)
                     /locus_tag="P5310_00845"
     CDS             complement(1079..1411)
                     /locus_tag="P5310_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU31942.1"
                     /translation="MKIDVKELLEDKVKSKSSHYVETSESMEGLTFDVLPRVWFTLER
                     VRDRKVLLTCKVETSVLLECSRCLELYPQYLGFSYEELYTEEPFRVIGKLTEEDFKFT
                     IENNIKLL"
     gene            complement(1422..2612)
                     /locus_tag="P5310_00850"
     CDS             complement(1422..2612)
                     /locus_tag="P5310_00850"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HRU31943.1"
                     /translation="MKILVVNCGSSSLKYQLFDMEKEEVIGKGTVERIGTPPSYLVYS
                     INAEKIRKEVSADNHTEAFKVVIDALLNGDKSVISSIDEISAVGHRIVHGGERFKEPT
                     IIDEEVIAEIEKNNELAPLHNPAGLSGIRASIEVLSSVPQIAVFDTSFHSKMPRYAYL
                     YAIPMRFYRDYRIRRYGFHGTSHYYVAQRVAELVGKPIEELKIVTCHLGNGCSISAVK
                     GGRSVDTSMGFTPLEGLIMGTRSGDLDPAIPLYLMRNLNYTAEDVDNILNKESGLLGI
                     SEFSNDMRDVMKAAEEGNQNAKLAMEMFCYRIKKYIGSYAAIMNGLDVLVFTAGIGEN
                     EPEIRAIVCQDMDYLGINIDLQRNNFKGQEREISTPNSKVKVWVIPTNEELVIARETA
                     RLIS"
     gene            complement(2628..3641)
                     /gene="pta"
                     /locus_tag="P5310_00855"
     CDS             complement(2628..3641)
                     /gene="pta"
                     /locus_tag="P5310_00855"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HRU31944.1"
                     /translation="MGEPVNIMLRIRERAKKANKKVVLPESFEERTLKAAEMATREGI
                     ARIVLIGEESAIKERARELNVSLDGIDIINPDVYPEKELLATTLVELRRSKGIDTLDK
                     AYKVLEDPLYFATMLLKLDKVDGWVAGAVHSSPDAERPIFQIIKTAPGIDVASGAFLV
                     VVPDCPYGENGIFVYADCGVNPSPNANELASIAISSAQLFRILVEKEPKVAMLSFSTK
                     GSAKHESVDKVIEATNIARSKAPDLIIDGELQGDAALVPSVGNSKAPGSPVAGKANVL
                     IFPDLNAGNICYKLTERLAKAKAIGPILQGTAKAANDLSRGCSAEDIVDSIAIVCVRA
                     TAL"
     gene            complement(3657..4478)
                     /locus_tag="P5310_00860"
     CDS             complement(3657..4478)
                     /locus_tag="P5310_00860"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HRU31945.1"
                     /translation="MKFKIPFDPIYTKDILVRFIRDETRKFGIDKLVIGLSGGLDSSV
                     SATLGVDALGRSNILGYVLPYRTTSQNSIDDAILMAEMLGVNTELIDITPMVDAYFKG
                     ETEDRVRRGNFIARTRMCVLFDKSYQHSALVLGTSNKTELLLGYGTIYGDMASAINPL
                     GDLYKTQVRMLAKEIGVPDKIIAKPPTADLWVGQTDEGELGLTYEEADEILYRLVEER
                     IRPELLLEMGYREESIDKIVYLMRKNQYKRTLPVIAKLSCRTIGIDFKYPRDWNN"
     gene            complement(4468..5301)
                     /locus_tag="P5310_00865"
     CDS             complement(4468..5301)
                     /locus_tag="P5310_00865"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HRU31946.1"
                     /translation="MKLRVHLAQFYPKLGNIEYNMEKHIEISGRAKDKADLLIFPELS
                     LTGSNLMDLVSEVALEKNSKILNPLLEFSSNLDIIFGLVELGENNLFYDSAFYCSKSS
                     LKYVHRKSFLSSPRRSGGKDYFSSGNNTGVLNADWGKLGFIISDDALNPVSVLPFIQK
                     NPDIIIIIDNGLSFGFSSDTGIPKSALMWKNLISLYAQIFCSYVIYVNRVGFEDGLNF
                     FGGSSIVDPYGETIISCPYFEESSLDVELDLQIFKSRPFNLSFNRERLESILRNLKDE
                     I"
     gene            complement(5298..5780)
                     /gene="coaD"
                     /locus_tag="P5310_00870"
     CDS             complement(5298..5780)
                     /gene="coaD"
                     /locus_tag="P5310_00870"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015907719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HRU31947.1"
                     /translation="MKRAVYPGSFDPITNGHIDIIRRSVKLFDELIVAVLVNPAKSPL
                     FTVEERMELIKESLNDLPNVKVDSFTGLLVHYVSGVKANVIIRGLRAVSDFETEFQMA
                     LMNKRLNSDVEIVFLMTSYEYSYLSSGVIKEVFGLGGDVTGLVPECVEAKLLEKMRRK
                     "
     gene            complement(5749..6312)
                     /gene="rsmD"
                     /locus_tag="P5310_00875"
     CDS             complement(5749..6312)
                     /gene="rsmD"
                     /locus_tag="P5310_00875"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HRU31948.1"
                     /translation="MEIVITGGELRGRRLVVPDNIRVRPTMSLIRESVFNSLGDRVFN
                     SKFLDLFAGFGTVGIEALSRGAQSAIFVEKNKKSVGIIRKGLEIFDLSGRTEIFNISA
                     EKFLQITNSTYNIIYLDPPYMFDCSDLILGILDKKILADDGIIVWETSVRSNINKKRF
                     NTVKEKRYGETVVLYIEYEESGISREF"
     gene            complement(6315..>7523)
                     /locus_tag="P5310_00880"
     CDS             complement(6315..>7523)
                     /locus_tag="P5310_00880"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HRU31949.1"
                     /translation="QRLLQGDVGSGKTVVAAFFLYAVASNGYQGAIMAPTEILAQQHY
                     DRIGPLLSNLGIESALLIGSTKEKEREEIKLGLSQGKIPVIIGTHALIQEDIEFKNLV
                     GIVIDEQHRFGVMQRMALRNKGASPHFLVMSATPIPRTLALTLYGDLDISVIDELPRG
                     RLPVATYWASGEQRAQVYNMVRKLVKNGQQAYIVCPLIEESDKLEAESAVKLAKEMQE
                     EVFPEFRVGLLHGKMGINEKEEVMNRFRNGDIDILVSTTVIEVGVDVPNATIMVIENA
                     ERFGLSQLHQLRGRVGRGSLKSYCILISDLTSEDARERIKIMTRTQNGFEIAEADLSL
                     RGPGELNGIRQHGIPEFKVGNIIRDTRILELAREEAILLLKKDPELSSFERLKEMIEN
                     SPFTEFLSTG"
BASE COUNT         2210 a         1829 c         1145 g         2329 t
ORIGIN      
        1 ttaccgataa aaccatcaca gacaaccaca tctgccttat tctcaaatat atcattcccc
       61 tctatattcc caacaaaatt gggaatactc tttagaattg tataggtaga tttagtcaga
      121 gagttaccct ttgtttcctc tgaacctata tttataagtc caaccctggg gttatcaatc
      181 ccgagtatac tgtgtgagta actcataccc attattgcga actggtagag ataaacaggt
      241 ttacaatcaa tattggcacc ggtatcaatg ataaatacag gatgattcaa cgtgggaata
      301 gttataccta tcgacggtct ttctatccca gatatggtat ccagaattaa agatgcacag
      361 gctacaaagg cacctgtatt cccagctgat acaactgcat ctacctgttt atctcgcaac
      421 atctctaccg ccaagactaa agatgccctt tttttactct ttacagcctg agcaggatgc
      481 tcattcatct ggatagcctc ttcagtatga ataatctcaa aaggaagctc tctgtttcca
      541 gctatctctc gaattttcgc ctcatcacca accagaacca attccacatc gagattatcc
      601 ttcgctatat atgctccttc taccagaacc tgtggggcaa aatccccacc catagcatct
      661 aaggctattc tcatttctcc tcttttatag ttagaaccac ttcaccgtca tagtaaccac
      721 aagaaggaca taccctgtgg ggcaaggtcc actcatggca ctgggggcaa cgtattacag
      781 aaggaggact catcttccag agagacctcc ttctatgcgt tctgcatctg gacattttct
      841 tttttgggtt agccatatat caatccctcc atcaaagttc tatttagcct agtatttaat
      901 aacagtatct cctaaactac aattacaact ctctctatta agattcttcc cacatacagg
      961 acagagccct ctacaatcat ctccacataa aggcttaata gggagattta atattatatt
     1021 ctccctcaca ctgttagaga tatctatnnn nnnnnnntta aagctgttga gagaaatttt
     1081 agagcagttt tatattgttt tctattgtaa acttaaaatc ttcttctgtc aattttccaa
     1141 ttaccctgaa tggctcttca gtatagagct cctcatacga aaatcccaga tactgcgggt
     1201 aaagttccag acatctactg cattcaagaa gtacactggt ttcgacctta caggtaagaa
     1261 gtacctttct gtctctaact ctctccaatg taaaccatac tctgggcaaa acatcaaaag
     1321 taagtccttc catagattct gatgtttcta cataatggga agacttactt ttgaccttat
     1381 cctctaacag ctcttttaca tcaatcttca ttttacttaa atcaactgat taacctggct
     1441 gtctctcttg caataaccag ttcttcgtta gttgggataa cccacacttt caccttagaa
     1501 ttcggtgtac ttatctctct ttcctgccct ttaaaattat ttctctgaag gtctatattg
     1561 ataccaagat agtccatatc ttgacataca atagccctta tctctggttc attctcccct
     1621 ataccggcgg taaatactag gacatccaga ccattcataa tagctgcgta ggaacctata
     1681 tacttcttta ttctatagca aaacatctcc atagccagct tggcattctg attaccctct
     1741 tcagcagcct tcattacgtc tctcatatca ttgctaaact cagaaatacc gagaagacca
     1801 ctctccttat tgaggatatt atccacatct tcagcagtat agtttaaatt tctcataaga
     1861 taaagaggta ttgcaggatc aaggtcacca cttctggtac ccataataag tccttcaagt
     1921 ggagtaaatc ccatactggt atctacagac cttccacctt tgacggcaga tatactgcaa
     1981 ccatttccga gatgacaggt aacaatcttt aattcttcta tcggcttacc aacaagttct
     2041 gccacccttt gagcgacata atagtgactg gttccgtgga aaccatacct tctaattcta
     2101 taatctctgt aaaacctcat aggaatagca tagagatagg catacctggg catcttcgaa
     2161 tgaaaagaag tgtcaaaaac cgctatctga ggtacactgg aaagaacctc tatactggct
     2221 cttataccag aaagaccggc aggattatgc aggggagcca gttcattatt tttttctatc
     2281 tcagctataa cctcttcatc tataatagta ggttctttaa atctctcgcc tccgtggact
     2341 atcctatgac ctaccgcact gatctcatct atagaactta taaccgactt gtcaccattt
     2401 aaaagggcat caataactac cttaaaagcc tcggtatgat tatcagctga tacctccttt
     2461 cttatctttt ctgcatttat cgaatagaca agataagaag gaggagtccc tattctctcc
     2521 actgtaccct tgccaataac ctcttccttc tccatatcaa acaactgata tttaagagaa
     2581 gaactcccac agtttacaac taatatcttc ataatatgta taacctccta aagagctgtt
     2641 gctctcacac atacaatggc gatagaatcc actatatctt ctgcactgca tcccctggaa
     2701 agatcattgg cagctttagc tgttccctgg agaataggac ctatagcctt tgccttcgcc
     2761 aatctttctg taagcttgta acatatattt cctgcattca aatccgggaa tataagcaca
     2821 ttagcctttc ctgccacagg acttccaggg gctttagaat ttccaacact tggtaccagg
     2881 gcggcatctc cctgaagctc tccatctata atcagatcag gggctttact cctggctatg
     2941 ttagtcgcct ctattacctt gtccactgat tcatgttttg cgctcccttt ggtggaaaaa
     3001 gagagcatag ccactttcgg ttctttctct accagtattc taaaaagttg ggcgctggaa
     3061 atggctatag atgcaagctc attcgcattc ggactggggt ttaccccaca atctgcataa
     3121 acaaatatcc cattttctcc ataaggacaa tcgggaacca ctacgaggaa ggctccagat
     3181 gccacatcga tacctggagc agtttttatg atctggaaaa taggtctctc tgcatccgga
     3241 ctggaatgaa cggctcctgc tacccaacca tccactttat ccagttttaa taacatggtc
     3301 gcaaaatata gaggatcttc caggacctta taagccttat ccaaagtatc tataccttta
     3361 gaccttctca gttcaactaa ggtagtggcc agtaactctt tctcaggata gacatcagga
     3421 tttataatat caattccatc caaacttaca ttgagctctc ttgctctctc tttaattgct
     3481 gactcctcac ctatcaagac aatccttgct atcccttctc tggtagccat ttcagctgcc
     3541 tttaaagtcc tttcctcaaa actctctgga aggacaacct ttttattggc tttttttgcc
     3601 ctttccctta tcctcaacat aatattaact ggttcaccca taaatcaaaa acctccttaa
     3661 ttattccaat ctctaggata tttaaaatca atccctatag tacgacagga caatttggct
     3721 ataaccggta aagtacgttt atactgattt tttctcatta aatagacaat cttatctata
     3781 ctttcctctc tataacccat ctctaataat aattcagggc gaattctctc ttctaccagc
     3841 ctatacagta tctcgtccgc ctcttcatat gtaagaccta gctctccctc atctgtctgt
     3901 ccaacccata gatcggcagt gggtggtttt gctattatct tgtctgggac tccaatctct
     3961 ttagccagca ttcttacctg agttttataa agatcaccca gaggatttat agccgatgcc
     4021 atatcaccat atatagtacc gtatccgagc agtaattccg ttttattact cgtccctaaa
     4081 actaaagcac tatgctggta ggatttatca aaaagtacgc acatcctggt tctagctata
     4141 aagtttcctc ttcttaccct gtcctcagtc tcacccttaa aatacgcatc aaccataggt
     4201 gttatatcta ttaattcagt atttacgccc aacatctcag ccattaaaat ggcatcatct
     4261 atactatttt gactggtagt cctgtaagga agtacatagc ccaggatatt actcctgccc
     4321 aacgcatcta cacctaaagt cgcagacacg ctggaatcca gcccgccaga aagtcctatc
     4381 acaagtttat caataccaaa ctttctggtt tcgtccctta taaatctaac aagtatatct
     4441 ttagtgtaaa taggatcaaa tggaatttta aatttcatct ttaagattcc tcaaaatcga
     4501 ttctaacctc tcccggttaa atgacaggtt gaaaggtctg gatttaaaaa tttgcaggtc
     4561 cagttccaca tcaagtgaag attcctcaaa ataagggcag gaaataatag tctctccata
     4621 tgggtctact atagatgaac ctccaaaaaa gtttagccca tcttcaaaac ctactctatt
     4681 tacatagatg acataagaac agaatatctg tgcataaaga gagatcagat ttttccacat
     4741 cagagcgctc tttggtatac ctgtatcaga agaaaaacca aaagataaac cattatcaat
     4801 tattataatt atgtcaggat tcttctggat aaaaggtaat acagatacag gattcagtgc
     4861 atcatcagaa ataataaatc caagttttcc ccaatctgca ttcaaaacac ctgtattgtt
     4921 cccactagag aaataatcct tcccacccga ccttctggga gatgacagga aagacttacg
     4981 atgtacgtat ttaagggaag acttactgca gtagaaagct gaatcgtaaa aaagattatt
     5041 ctctcccagt tctacaagtc cgaagatgat atccagattg ctggagaatt ccagcaaagg
     5101 gttgaggatt ttactattct tttctaaagc aacttcagaa accaggtcca taaggttaga
     5161 cccggtaaga ctcagttctg gaaatattag aaggtcagcc ttatcctttg ctcttccaga
     5221 aatttctata tgcttctcca tattgtattc tatattacca agctttggat aaaactgagc
     5281 cagatgtact cttaacttca tttccgcctc atcttttcca acaacttagc ctcaacacac
     5341 tccggaacaa gtccggttac atcccctcca agtccaaaaa cctctttaat gactccagaa
     5401 ctcaaataag aatactcata gctggtcatt aagaaaacta tctcaacatc cgaatttaat
     5461 cttttattca taagcgccat ttgaaattct gtctcaaaat ctgatacggc tctaagacca
     5521 cgtattatca cattggcttt tactcctgag acatagtgaa ccaataagcc agtaaaactg
     5581 tccaccttta cattaggtag atcattcagg ctttccttta tcagttccat cctctcttcc
     5641 actgtgaaga gaggagactt tgccggatta acaaggacag caacaataag ctcatcaaag
     5701 agctttactg atcttcttat tatatctatg tgtccgttcg ttataggatc aaaactccct
     5761 ggatataccg ctctcttcat actctatata taaaaccacc gtttctccat atctcttttc
     5821 ttttactgta ttaaatcttt ttttattaat attgcttctt acagaagttt cccatactat
     5881 aataccatca tctgcaagta tctttttatc cagaatcccc aaaatgaggt ctgagcaatc
     5941 aaacatatat gggggatcaa gataaatgat attatacgta gaattcgtaa tctgtaaaaa
     6001 cttctccgca gatatattaa aaatttctgt acgaccagag aggtcaaata tttctagtcc
     6061 ctttcttatt atacccacag attttttatt cttctcaaca aaaatagcag attgagcccc
     6121 cctactgaga gcttctattc ccaccgtccc aaaaccggca aaaaggtcca aaaatttaga
     6181 attaaaaact ctatctccca gagaattaaa gacggattct cttataagac tcatagtggg
     6241 tctaacacga atattatccg gtaccaccaa tctcctaccc cttaattctc ctccggttat
     6301 aactatttcc atccctaacc tgtacttagg aattccgtaa agggagaatt ttcaatcatc
     6361 tctttgagtc tttcaaaaga agatagttcc ggatctttct ttaaaagaag aatcgcctct
     6421 tctcttgcca attcaagaat tctcgtatcc cggataatat tcccaacctt aaattctggt
     6481 ataccatgct gacggattcc gttcagctct cctggacctc gtaaacttag atcagcctct
     6541 gctatttcaa atccgttctg agtcctggtc attatcttta ttctttctct ggcatcttct
     6601 gaagttaaat cacttataag gatacagtaa gacttcaaac tacctctacc aacccttccc
     6661 cttaattgat gaagctgaga gagcccaaat ctctctgcgt tctctataac cataatggtt
     6721 gcattgggta catctacccc aacctctatc accgtggtag aaacaagtat atcaatatct
     6781 ccattcctaa atctattcat aacctcttct ttctcgttaa tccccatctt tccatggagt
     6841 aaaccaactc tgaattcagg gaatacttct tcctgcatct ctttagctaa tttaacagct
     6901 gattctgcct ctaatttatc tgattcctct ataagaggac aaacgatgta agcctgctga
     6961 ccattcttta ccagtttcct caccatatta taaacctgtg ccctctgttc accagaagcc
     7021 cagtacgtgg caaccggtaa tctaccacgg gggagttcat ctattacaga gatatctaaa
     7081 tctccataca gtgttaatgc taaggtccta ggtattggag tagcgctcat aacgagaaaa
     7141 tgaggtgatg cccctttatt tctcagtgcc atcctctgca taactccaaa gcgatgttgt
     7201 tcatctataa ctattccaac cagattttta aactcaatat cttcctgaat aagggcatga
     7261 gtaccgataa taacagggat tttgccctga cttaacccta actttatctc ttctctctcc
     7321 ttctctttag tggaacctat aagcaatgca gattctatgc ctaaattgga cagaagagga
     7381 cctatgcggt cataatgctg ctgggctaaa atctctgttg gagccataat agccccttga
     7441 tatccgttag acgctaccgc ataaaggaag aaggctgcaa ctaccgtctt gccggatcct
     7501 acatcaccct gcagaagtct ctg
//
LOCUS       DAOTKU010000017         7368 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_9784_length_7368_cov_3.288801, whole genome shotgun sequence.
ACCESSION   DAOTKU010000017 DAOTKU010000000
VERSION     DAOTKU010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7368
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9784_length_7368_cov_3.288801"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /locus_tag="P5310_00885"
     CDS             complement(<1..490)
                     /locus_tag="P5310_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013291260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HRU31950.1"
                     /translation="MLEKLREEVCEMNLELSRSRLVTMFSGNVSGRDPETGYVAIKPS
                     GMNYRNMRPEDMVIVDMNGDVVEGKHKPSVDTLTHLYIYKNRSDVNGITHTHSNYATS
                     FAALGQSIPVYLTAMADDFGGPVPCTDYAPVGSEAIGKEILRVIGNSPAVLVKNHGVF
                     TIR"
     gene            complement(494..2446)
                     /locus_tag="P5310_00890"
     CDS             complement(494..2446)
                     /locus_tag="P5310_00890"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HRU31951.1"
                     /translation="MFYYGVDYYPEHWPEERWNLDASLMESAGINVARLAEFAWVKME
                     PEEGHYDFSWLDRAIEVLGKHRIKVVLGTPTASPPMWLVKKYPQILPVDRDGHIMGPG
                     GRRHYCYNSPEYRELTRKIVTAMAEHYKDNPNVIGWQIDNELGCHESNRCYCDNCLIA
                     FREWLKKKYGTLENLNNSWGTIFWSQTYTSWDEIIIPRRTVGSHNPSLLLDFLRFVSD
                     STIDYQNLQIEILRRTCPDKFITHNLMGLFGGLDYYKLAETLDFISWDNYPKFRGDVN
                     PASIALSHDVMRGVNSGKPFWVMEEQSGAAGWEIFGPHPLPGEIRLWAYQGIAHGADA
                     IVYFRWRTCRFGTEEYWHGILDHDGVPRRRYEEVKKMGEELKNLTDLVGSRYKAEVAI
                     IYSYDIRWAFDIQPNNPSFNYMEQIQRYYAGLFNQHVPVDIVNPLSDLSRYKLVIAPS
                     LYLMTPQIRENLERYVREGGTLVVTPRSGVKDWNNVVTDKTLPGELADLLGVEIEEYY
                     SIPGDERYQIKGIGPEKYSCKAICELISPKSAEVIAQYEDGPYAGKPAATINRFGNGE
                     AIYIGADVDIQYIESLLFWLITRCRIDIIVMGPKEVEVTVREGEDGVFIFLLNHSDKA
                     EAVKLSFPGSIEEIMLEPGDVKIIKR"
     gene            complement(2465..4024)
                     /gene="lsrK"
                     /locus_tag="P5310_00895"
     CDS             complement(2465..4024)
                     /gene="lsrK"
                     /locus_tag="P5310_00895"
                     /EC_number="2.7.1.189"
                     /inference="COORDINATES: protein motif:HMM:NF008187.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071518 - autoinducer-2 kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
                     /protein_id="HRU31952.1"
                     /translation="MKKYIVTLDAGTGSGRCLIFDLEGNQISQAQEEWIPKEIPEYPG
                     SQEFDTKEAWTILCRTIKTAMAKADIKPEEVLAITASSMREGMVVYDKEGNEIWACTN
                     LDARAVEEAVELIKSGDAEKIFKLGGDWVNIIDPPRFLWIKKHQPEVFEKIGHMNMLS
                     DWVLYKLSGKIITDPSIGSSSGMFDLARRTWADETIRLVGLSKDIFPPVYESGTIIGE
                     VTDQAGRDTGLLPGTPVVTGGADTQLALVGVSAVENNRYVVVGGTQWQTAVVTDKPLI
                     DPKFRVRTLCHSMPGFWMTEGIGFYHGFSTRWFRDAFCQLEKEMAKQMGVDPYYVLEK
                     LASQASPGSNGIIAIFSDVMNAKRWKHAAPSFLQFDILSPQTSGKKECYRALEENAAY
                     VSYGNYQILLELTKSPAEEIVFCGGSSKGFLWPQIMADVYGVKIKVPVVKEATSLGSA
                     MIAGVAMGVYKNLEEAVERLVRFERVYEPDMGNHKIYQDYYKKWREIYPRILQLVDEG
                     LLKPMWRAPGT"
     gene            4187..5158
                     /locus_tag="P5310_00900"
     CDS             4187..5158
                     /locus_tag="P5310_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012582676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HRU31953.1"
                     /translation="MAYFDMSLEELRRYLPERYEEEDFDSFWKETLTEAENYPLNPLF
                     EKVDYHLETVETFDVTFSGYKGQRIKGWLIIPRIREKKLPCIVEFIGYGGGRGFPYEW
                     LLWSSVGYAHLVMDTRGQGSSGSPGDTPDYEEEISNPQYPGFMTRGILNPKAYYYRRV
                     FTDAVRAVETACTFPDIDPEKIAVCGGSQGGGISLAVSGLSPRVKALMSDVPFLCHYR
                     RAVQIIDEMPYGEISRYCKVHRDKIETVFRTLSYFDGVNFATRASCPAIFSAGLMDTI
                     CPPSTVFAAYNYYGGEKEIKVYPYNNHEGGGTFHTLEKIKFIKRIFG"
     gene            5162..6259
                     /locus_tag="P5310_00905"
     CDS             5162..6259
                     /locus_tag="P5310_00905"
                     /inference="COORDINATES: protein motif:HMM:TIGR03942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HRU31954.1"
                     /translation="MVKNDLTLLIKPTSAECNLGCKYCFYSPKENLYQDKDKRMSSDI
                     LEITVKKALDIAENITFCWQGGEPTLMGLPFYKRVVELQKRYKKDDQSISNAFQTNGV
                     LLNEDWALFFRENNFLVGLSVDGPSSVHDEYRITKDGKPTHHLVLSKLKLLQGYNVAF
                     NILTVVNDKNSKKPESLFKYFRELGVEYMQFIPCLEKGREGKIASFSVDPNSYGNFLC
                     TMFDKWYNNGKPECYIREYEEWLIAYAYGINPCMFKPYCEGALVIEYNGDIYPCDFFV
                     EPEWFLGNIKEVSLEEVLNSEPYRKFKERKHMVDERCIKCKFLNLCWGGCPKFRINKK
                     GKAINYSYYCNSYQRFFAYADRYFKSLIKTI"
     gene            complement(6252..>7368)
                     /locus_tag="P5310_00910"
     CDS             complement(6252..>7368)
                     /locus_tag="P5310_00910"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HRU31955.1"
                     /translation="DVVGANCSMGSDGLYRVLERMVLSGVPYISIQPNAGLPIIEDGK
                     LIYPETPEQLAEFAVKYVELGASIVGSCCGSTPEHTKAISNAVRGLKRTAIRKRDSYL
                     AFTSRTRLIEVGKGLPVAVIGEKLNAYAEECKSLLDAKDMDGLVELGREQVQSGARAI
                     DMHIPVSSTVEREFIRELMINFQQFGDVPLVFDIQEPDTLEMALRIYPGRALINSISL
                     EPSREGIIDLARKYGAAIVGLTSGRYKLPADADERIKNGEELISLFNPEDRVDIVLDP
                     LVFSVGVSPEQALETLKALEHFNSKGYLTIIGLSNISYGLPKRSLLNRIFLSMAVSNG
                     LDSVILDPTDKDLMETLVASQTITGRISVLDYIKKFK"
BASE COUNT         2188 a         1680 c         1340 g         2160 t
ORIGIN      
        1 tccttattgt aaaaacacca tgattcttaa ctaaaacagc cggactatta cctataactc
       61 tcagtatctc tttcccaata gcttcacttc caacaggagc gtaatccgta caagggacag
      121 gcccaccaaa atcgtcagcc atcgctgtca ggtatacagg aatagattgt cctaaagcag
      181 cgaaacttgt tgcatagtta gagtgtgtat gggtaatacc attcacatcc gatctattct
      241 tatagatata gagatgggtg agtgtatcga ctgaaggctt atgtttaccc tccactacat
      301 ctccattcat atcaacaata accatatcct ctggtctcat atttctataa ttcattccac
      361 ttggctttat ggcaacataa cctgtttcag gatctcttcc actcacattc ccactgaaca
      421 tggtaactaa cctgcttcgc gataattcaa ggttcatctc acatacttct tctctaagtt
      481 tctctaacat tgctcacctc ttgattatct ttacatctcc tggctcaagc attatctctt
      541 ctatactacc ggggaaagat agttttacag cttcagcttt atcgctatga tttagcagga
      601 atataaatac tccatcctcc ccttctctta cagttacctc tacctcttta ggtcccataa
      661 ctattatatc aattctacat ctcgttataa gccagaagag gagagattct atatactgga
      721 tatccacatc tgccccaata tatatagctt ctccattacc aaatctattt atagtagcag
      781 cgggttttcc agcatatggt ccatcctcat actgagcaat tacctctgca gattttgggc
      841 ttataagttc acatatagcc ttacaggaat acttttccgg tcctattccc tttatctgat
      901 atctctcatc ccctgggata gagtaatact cttcgatctc cacccccaaa agatctgcca
      961 gttcaccagg gagagtcttg tcggtaacta cgttattcca gtcctttacc cctgaccttg
     1021 gggtaacaac tagtgtgcct ccctctctga catatctctc aaggttctcc cttatctgtg
     1081 gggtcataag gtacaggctg ggagctataa ccagtttata tctggacaga tcgcttaatg
     1141 gatttactat atccactgga acgtgttggt tgaatagtcc agcgtaatac ctctgtatct
     1201 gttccatata attaaaggat ggattgtttg gttgtatatc gaaagcccat cttatatcat
     1261 aggaatagat tatagccact tccgccttgt aacgagaacc caccagatca gtgaggtttt
     1321 taagttcttc ccccatcttt tttacctctt cataccttct ccttggaacc ccatcatggt
     1381 ccaatatacc atgccagtat tcttcggtac caaatctgca tgttcgccat ctgaaatata
     1441 caatagcatc tgctccgtga gctataccct gataagccca gagcctgatc tctccaggta
     1501 aaggatgagg gccaaaaatc tcccaaccag ccgctccact ctgctcttcc atcacccaga
     1561 agggttttcc actattcaca cccctcatta catcatggga aagagctata cttgccgggt
     1621 taacatcccc tctaaatttg ggataattat cccaggatat aaagtctaga gtttcagcca
     1681 gtttataata atcaagtcca ccaaaaagtc ccataagatt atgcgtgata aatttgtccg
     1741 gacaggtcct tctcaatatc tcaatctgaa gattctgata atctatggtg gaatctgata
     1801 caaaacggag aaaatctagt aataaactgg gattatgaga acccactgtt cttcttggaa
     1861 ttataatctc atcccagctt gtatatgtct gactccagaa gatagttccc catgagttat
     1921 tcaggttttc aagtgttcca tatttcttct tcaaccattc tctgaaagca ataagacagt
     1981 tatcacaata gcatctatta ctctcgtgac aacccagttc attatctatc tgccagccta
     2041 taacatttgg attatctttg taatgttccg ccatagcagt aacaatcttc ctggtaagtt
     2101 ctctatactc cgggctatta taacagtagt gtctccttcc tccaggaccc attatatgcc
     2161 catctctgtc aaccggtaaa atctgcgggt atttcttcac aagccacata ggaggacttg
     2221 cagtgggggt acctaaaaca acctttatcc tgtgcttacc taagacttca atagctctat
     2281 ccagccagga aaaatcataa tgaccttcct ctggctccat cttaacccag gcaaactcag
     2341 caagccttgc gacatttatc ccggcagact ccataagact ggcatccaga ttccatctct
     2401 cttccggcca atgttcagga taataatcca ctccataata aaacatagct ttttcctcct
     2461 atagttatgt cccaggtgct ctccacatag gcttcagtaa tccttcgtct acaagctgga
     2521 gtatcctagg gtatatctct ctccacttct tatagtaatc ctgatatatc ttatgattac
     2581 ccatatcagg ctcatacacc ctctcaaacc tcaccagtct ctccaccgcc tcttccaggt
     2641 tcttataaac ccccatggca acgccggcaa tcatagctga acccaatgaa gttgcctctt
     2701 ttaccaccgg gaccttaatc tttacaccgt aaacatcagc cataatctgt ggccataaga
     2761 atcctttaga agaccctcca cagaagacta tctcttcagc aggtgatttt gtaagttcaa
     2821 gtaagatctg ataatttcca taagaaacat aagctgcatt ttcttccaac gccctgtaac
     2881 attctttctt accggaggtc tgtggggaaa gtatatcaaa ctggaggaag gatggagctg
     2941 catgcttcca tctcttggca ttcataacat cagagaatat agctatgata ccgttagacc
     3001 ctggagacgc ctgggaagca agcttttcca gaacatagta tggatccaca cccatctgct
     3061 tcgccatctc tttctccaat tgacagaaag catccctgaa ccatcgagta gagaaaccat
     3121 gataaaagcc aatcccctct gtcatccaga agccgggcat actatgacaa agggtcctta
     3181 ccctgaattt cggatctata agaggtttat cagtaaccac tgctgtctgc cactgggttc
     3241 caccaaccac cacatatcta ttattctcaa ccgcactgac acctaccagt gcaagctgag
     3301 tatctgcacc tccagtaacc accggagtcc ctggtagtaa tccggtatct ctacctgcct
     3361 gatcagttac ctcccctatt attgtcccgg actcatacac tggagggaag atatccttac
     3421 ttaagccaac aagccggatg gtctcatctg cccaggttct cctggcaaga tcaaacatac
     3481 ctgaactgga gcctattgaa gggtcggtta tgattttacc tgaaagttta tataagaccc
     3541 agtcactcag catattcata tgtccaatct tttcaaatac ctctggctga tgcttcttta
     3601 tccataagaa tcttggaggg tctattatat tcacccagtc cccaccaagt ttaaagatct
     3661 tctctgcatc gccagacttt ataagttcaa cagcttcctc caccgctctt gcatcaagat
     3721 ttgtacatgc ccagatctca ttcccttctt tatcgtatac taccatcccc tctctcatac
     3781 tgctcgctgt tatagctaag acttcctccg gttttatatc agccttagcc atagcagtct
     3841 taatagtcct gcagaggata gtccatgctt cttttgtatc gaactcctga gagcctggat
     3901 attctggaat ctcttttggt atccactcct cctgagcttg agatatctga ttgccttcca
     3961 gatcaaagat aagacacctt ccactaccgg ttcctgcatc aagggtaaca atatattttt
     4021 tcatatttac acccctttcc taaaaacttt atgattaatt ataccagata agataaaaag
     4081 aggctacacc agcacacatt gaaaagatat ctgacttcca gtataacaat tattcagaca
     4141 tcgtgataaa atatctatta aaaatcaaag ataggaggat aaaattatgg cttacttcga
     4201 catgtcgttg gaagaattaa ggagatatct tccagaaaga tatgaagagg aggattttga
     4261 ctctttctgg aaagaaacac ttacagaggc agaaaattat ccgttaaatc ctttatttga
     4321 gaaagtggat taccatctgg aaacagttga gacgtttgat gttaccttct ctggatataa
     4381 agggcagaga ataaagggct ggttaataat acccaggatc agggagaaaa aattaccctg
     4441 tattgtagaa tttattggtt atggaggagg gagaggtttc ccctatgagt ggttgctctg
     4501 gagcagtgtt gggtatgccc atctggttat ggatacaagg ggacagggaa gtagtggaag
     4561 cccaggagat actccagatt atgaggagga aatctcaaat ccacagtatc caggatttat
     4621 gaccaggggg attctaaatc caaaggctta ttactacaga agagttttta ctgatgcggt
     4681 aagagcagta gagaccgcct gtacatttcc agatatagat ccagagaaga ttgcggtatg
     4741 tggcggaagt cagggtggag ggatttcatt agcggtaagt ggattatctc caagggtgaa
     4801 ggcacttatg agtgatgtcc catttctatg tcattataga agagcggtgc aaataataga
     4861 cgaaatgcct tatggagaga ttagcagata ttgtaaggtg catagagaca agatagagac
     4921 agtctttaga actctctcat actttgatgg agtaaatttt gctacacgtg caagttgtcc
     4981 agccatattc tctgcaggtc ttatggatac tatctgtcca ccatctactg tatttgctgc
     5041 atacaactac tatgggggag agaaagagat taaagtgtat ccatacaata atcatgaggg
     5101 tggagggaca tttcatacac tggaaaagat aaaattcata aagagaatat tcggataaaa
     5161 gatggtaaaa aatgatttaa cactgcttat taaacccaca tccgcagagt gtaatctggg
     5221 atgtaaatac tgcttctatt caccaaaaga aaatctgtac caggataaag ataaaaggat
     5281 gtcatcagac atcttggaga ttactgtaaa gaaggctctg gatatagctg aaaatatcac
     5341 cttttgctgg cagggagggg agcccactct aatgggtctt cccttctata aaagagttgt
     5401 ggaactgcaa aagagatata aaaaggatga tcaaagtata agtaatgcat ttcagacaaa
     5461 tggagtactt ctaaatgaag attgggctct atttttcagg gagaacaact ttctggtagg
     5521 gctgagtgtt gatggtccat ccagtgtcca cgatgaatat agaattacaa aagatggaaa
     5581 acctacccat cacttagttc tcagcaaatt aaaactactc caggggtata atgtggcgtt
     5641 caatatatta acagtggtta atgataaaaa cagcaagaaa ccagagtccc tatttaaata
     5701 ttttagagag cttggggtag agtatatgca attcatcccc tgtctggaaa aaggcagaga
     5761 gggcaagata gccagttttt ctgtagaccc caatagttat ggaaatttcc tctgtactat
     5821 gtttgataaa tggtataaca acggaaaacc tgaatgttac ataagagaat atgaagaatg
     5881 gctgatagca tatgcatatg ggataaatcc ctgtatgttt aagccttact gcgaaggggc
     5941 tcttgttata gagtataatg gagatatata cccctgtgac ttctttgtgg agccagaatg
     6001 gtttttaggg aatattaaag aggtctcact ggaagaagta ctgaatagcg aaccatacag
     6061 aaaatttaaa gagagaaaac atatggtaga tgaaagatgc attaaatgta aatttcttaa
     6121 cttatgctgg ggaggatgtc caaagttcag aataaataaa aagggaaaag ctataaatta
     6181 tagctactac tgcaattctt atcagagatt ctttgcgtat gcagatagat attttaagag
     6241 ccttataaaa actatttaaa tttctttatg taatccaata ctgaaatcct gcctgtaata
     6301 gtttgagatg caacaagtgt ctccattaga tccttatcag tagggtcaag tataacactg
     6361 tcaagcccgt tactcactgc catactcaaa aatatcctgt taagtaaact tctcttggga
     6421 agcccataag atatgttact taatcctata atagtaagat atcccttact gttgaaatgc
     6481 tccaaagcct taagagtctc cagtgcctgt tcaggactta caccaacaga aaacaccaga
     6541 ggatccagca ctatatcaac cctatcctca ggattaaata gagagattaa ctcttctcca
     6601 ttctttatcc tctcatcggc gtccgcggga agtttatacc ttccagaagt aagccctact
     6661 atagcagcac cgtattttct agccagatct ataataccct ctctggatgg ttctaatgag
     6721 atagaattta taagggctct accaggatat atcctgagag ccatctccaa tgtatcgggc
     6781 tcctgtatat caaacaccag gggaacatcc ccaaactgct ggaaatttat cataagctct
     6841 cttataaact ctctctcaac agtactggaa acaggtatgt gcatatctat agccctggca
     6901 ccagactgaa cctgttcacg cccaagttct acgagcccat ccatatcttt agcatcaagt
     6961 aatgacttac actcctctgc atacgcatta agtttttctc caataacagc aacaggtaat
     7021 ccctttccta cctctatcaa cctagttctg gatgtaaaag ccagataaga atctcttttt
     7081 cgaatagcag tcctttttaa tcctcttact gcgttactta ttgcctttgt atgctctggg
     7141 gtggaaccac aacaggaacc tacaatactg gctccaagtt ccacatactt tactgcaaat
     7201 tcagccagtt gctctggggt ctctggatat atcagttttc catcttctat gatgggcaaa
     7261 ccggcatttg gctgtatact tatataagga actcctgaga ggaccattct ttcaagaacc
     7321 ctgtaaagcc catctgaacc catactgcaa ttcgctccaa cgacatct
//
LOCUS       DAOTKU010000018         7358 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_9806_length_7358_cov_3.666575, whole genome shotgun sequence.
ACCESSION   DAOTKU010000018 DAOTKU010000000
VERSION     DAOTKU010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7358
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9806_length_7358_cov_3.666575"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            252..626
                     /locus_tag="P5310_00915"
     CDS             252..626
                     /locus_tag="P5310_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU31956.1"
                     /translation="MKRALSSTYYIEDNKYSFGLLFRYILIPFLIILFLVEPVYANSN
                     IKGLFTAVPWDKQLHFTWGFVLTVTLVALTGSFTTGIFTVILLGLTKEYFIDDFPDME
                     DFIYTFCGTLLGCLITSTLIQR"
     gene            738..2018
                     /locus_tag="P5310_00920"
     CDS             738..2018
                     /locus_tag="P5310_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018130515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine synthase"
                     /protein_id="HRU31957.1"
                     /translation="MERKWSFETLALHGGQEVDPYTLSRSVPIYQTTSYLFKDAEHAA
                     RLFAVEEEGYTYSRMMNPTVNTFEKRVALLEGGVGALATASGQAAELIAILNIAKEGD
                     EIVSSFSLYGGTYNLFASTFKRLGITAKFVDPSDPENFRKVLTSRTKAIYAETLGNPK
                     LDTLDIEAVADIAHNAGVPLIIDNTIPSPYLVRPIEYGADIVIHSATKFIGGHGTSIG
                     GIIVDSGKFDWKNGKFPEIAEPDPTYHGLNYIEKFGEAAYISKARLQLMRDLGACMSP
                     FNAFLFLQGLETLPIRMERHSQNAMEVAQFLKNHPKVSWVNYPGLPDNPYYNTAKKYF
                     NRNLFGAMIGFGVKGGLEAGKKFVNNVKLLSHLANIGDAKSLVIHPASTTHAQLTPEE
                     QLAAGVTPDFIRLSIGLEGIDDIIYDIDQALSKA"
     gene            2023..3129
                     /locus_tag="P5310_00925"
                     /pseudo
     CDS             2023..3129
                     /locus_tag="P5310_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012035751.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
     gene            3153..3740
                     /gene="metW"
                     /locus_tag="P5310_00930"
     CDS             3153..3740
                     /gene="metW"
                     /locus_tag="P5310_00930"
                     /inference="COORDINATES: protein motif:HMM:TIGR02081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HRU31958.1"
                     /translation="MRRDYEIIVGLIDKNARVLDLGCGNGELLALLEKEKNIRGKGIE
                     INGKRVSESISRGISVYQGDMEEVLPEYPDKSYDYIIISQTLQEIRNPELVITHSLRV
                     SRFVIVSLPNFGYWRIRLSILFKGQIPSSIGDFVYTWYETPDVHPLSIRDFEDYCKIK
                     GIRIVKAYYLAHDRMISICPNLFATLGIYMLTQSS"
     gene            complement(3728..4651)
                     /gene="cysK"
                     /locus_tag="P5310_00935"
     CDS             complement(3728..4651)
                     /gene="cysK"
                     /locus_tag="P5310_00935"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015290593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HRU31959.1"
                     /translation="MKIAESMRDLIGNTPLVRLNKIGGDIRASLVAKLEFFNPAGSIK
                     DRIGVSMIEEAERLGKINKDSIIIEPTSGNTGIALAWVCALKGYRCILVMPDTMSIER
                     RKLLKAFGAEIILTPGEKGMEGAIEEAERLAGTNPSYFIPYQFKNPANPEIHRKTTAE
                     EIWRDTDGKVDIVVAGVGSGGTITGIAEVIKPRKPSFRAIAVEPEKSPVLSGGKPGKH
                     KIQGIGAGFIPEVLNRDIIDEVIRVSDESAIETARRLAKEEGVLAGISSGAAVYAGIQ
                     IAKREENEGKLIVVILPDTGERYLSTELYED"
     gene            4808..5968
                     /locus_tag="P5310_00940"
     CDS             4808..5968
                     /locus_tag="P5310_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009457390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HRU31960.1"
                     /translation="MEVPIGKGRWATRSYGFEEISIVPGDITVDPELVDVSLEIGDFK
                     FEIPIIAAAMDGIVDPNFAEVMGKLGGLAVLNLQGIQTRYENPKEVLEEIVSAPPEKA
                     TEIMQKIYKEPVRDELVYKRIKEMKDSGIVTACSSIPQEAERMVKIAVEAGVDIFVVQ
                     STVITERHKAKSYTPLSLAKFTRETSVPVVVGNVVNFSAATELFETGISGILVGVGPG
                     GACTTRQVLGIGVPQVTAIADVAYARDIFYHRTGKYIPVIGDGGMNTGGDIAKAIACG
                     ADGVMLGQALARAEEAFGRGYHWGMAMSHSALPRGTRVHTGTTGTLKEILFGPAKTDD
                     GTQNLVGALKNSLGVCGALNLKEMHQVEIVIAPSIKTEGKILQQAQKVGMGK"
     gene            6237..7271
                     /locus_tag="P5310_00945"
     CDS             6237..7271
                     /locus_tag="P5310_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012582798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRU31961.1"
                     /translation="MATIKDVAEKAGVSVMTVSRVINNNKYVSQETREKVEQAMEELG
                     YIPNALAHGLITKKTHVLGLIVSDITNPFFTTIARGVEDTAIKNGFNTILCNSDEDVE
                     KEERYIELLLRKRVEGIILSPADCDKRRRIEQIISRKIPLVLIDRRISGLQIDRVYSD
                     SINGAYELTKYLIDLGHRRIGIIIGPRRISTAVDRVEGYKRALQEEGISMEEELIRWG
                     KKYSREDGYLNAIELLTLEHPPTAIFGGNRLITVGILRAIRELKLRIPDDISVVSFDE
                     VEDISVTNPFLTVISQNSYVMGVVATEQLLERIRGNNKSPEDLQKIVLQPRLVIRKSC
                     GSPKEEVMKE"
BASE COUNT         2224 a         1286 c         1649 g         2199 t
ORIGIN      
        1 tatagcccgg ggtaatttaa ctctcacttt aaatttacct atattttacc ccccacattt
       61 ctgtcaaggg gtttccgaga gactactcta tgcttatcta tcaaggtgtg cttgccttac
      121 tcttcaagcc tcaatacacc gttataggaa taataatact atagatatca aaatactaca
      181 agaactttct gaatattaat cgttggaaat gattagccag gaaaaatctt ttgagatata
      241 atatagggca tatgaaaaga gctttgagtt caacatatta tatagaggat aataaatata
      301 gctttggttt actgttccga tatattttga taccttttct tattattcta tttctggtag
      361 agccggtcta tgcaaattca aatattaagg gattatttac tgctgtcccc tgggacaagc
      421 agcttcactt tacctggggg tttgtcttga cagtaaccct cgtagcttta acaggctctt
      481 ttactacagg gatatttact gttatccttc ttggattaac caaagaatat ttcatagatg
      541 atttccctga catggaagat tttatatata ccttttgcgg aactcttctt ggatgtttga
      601 tcaccagcac attgatacag aggtagagaa atttacatgc tagaatattg tcctgggact
      661 tttcataaat ctttgactat ccttatagaa attagtataa tttcgttaca aaatactata
      721 agattcagga ggtaattatg gaaagaaagt ggagttttga aacattagct ttacatggag
      781 gacaggaggt cgatccgtac accttgtcca gatcggtacc tatataccag accacgtctt
      841 atttattcaa agatgccgaa cacgctgcaa gattatttgc agttgaagag gaaggttata
      901 catactccag gatgatgaat cctactgtta atacattcga gaaaagggtt gctctacttg
      961 aaggtggggt tggggcatta gctacagctt caggtcaggc ggcagaactt atcgctattt
     1021 taaatatagc caaggagggg gatgagattg tctccagttt tagcctgtat ggaggaacat
     1081 ataatctttt tgcctctaca tttaagagat taggtataac tgctaaattt gtagaccctt
     1141 ctgatcctga gaactttagg aaagttctta cttccaggac aaaagctatt tacgctgaga
     1201 cgcttggaaa tccaaagctt gatacacttg atatagaagc agttgctgat atagctcata
     1261 atgcaggggt tcctctcatt atagataata ctatcccctc accgtatcta gtaagaccta
     1321 tagaatatgg tgccgatata gtgattcatt ccgccacaaa gtttattggt ggacatggga
     1381 catcaattgg tggaataatt gttgacagtg gtaaatttga ctggaagaat gggaaattcc
     1441 ctgagattgc ggagccagac cctacatatc acggattgaa ttatatagaa aagtttggag
     1501 aggctgcgta tatttctaag gcaaggttac aattgatgag agacttaggg gcttgcatga
     1561 gtccattcaa cgcgtttctc ttcttgcagg gacttgagac attaccgata agaatggaaa
     1621 gacacagtca gaatgctatg gaagtcgctc aatttctgaa gaatcatcct aaggtcagct
     1681 gggttaatta tcctggtctt cctgataacc catactacaa cacagctaag aaatatttca
     1741 atcgtaatct cttcggtgct atgatcgggt ttggagttaa aggaggcttg gaggctggca
     1801 agaagtttgt aaataatgtg aaattattgt ctcatcttgc caatatagga gatgccaagt
     1861 ctttggtaat tcatcccgct agcactactc atgcccagct cactccggag gagcagctgg
     1921 cagcaggagt aacccctgac tttataagac tttccatcgg attggaaggt atagatgata
     1981 taatttatga tatagatcag gctttgagta aggcatagaa gaatggaaga ggttttttat
     2041 gtagagacac agtactatac ctttgcacat ccaccagacg aattggtact ggaaagtgga
     2101 gaaaaattag gaccgataac actggcgtat gaaacctatg gagaacttga taaaggtaaa
     2161 aagaatgcca tacttatact gcatgctctt tccggggatg cccatgtagc agggtaccat
     2221 aagggtgata gaaagcctgg ctggtgggac aatatggttg gtccaggtaa ggcttttgat
     2281 acaaataaat actttgtcat atgctccaat gtgattggtg gatgtaaggg ttcgacaggt
     2341 ccatcttcta ttaatccagc cacaggtaaa ccttatgggc ttgaattccc cgtgataaca
     2401 ataaaagata tggttaatgc ccaaaaatat cttatagatt acctcggtat tgagaagata
     2461 ttatgtatcg ccggtggttc aatgggtgga atgcaggtcc ttcaatggac tgtaagttat
     2521 ccccagctgg taaggtctgc cataccgata gcaacagcca gcaagaacag agccatgcag
     2581 attgccttca atgaggttgg aaggcaggct ataatgagtg atccaaactg gaataagggt
     2641 gattactatg gtaagtctca tcctgataca ggtttggcga ttgcccgtat ggttggtcat
     2701 atcacatatt taagtgaagt ttctatggag caaaagtttg gcaggagatt acgtcagaaa
     2761 gagaggtatg ggtatgattt ctcggtagat ttcgaggtgg aaagttattt acaccatcag
     2821 gggttgagtt tcacacagag atttgatgct aacagctatc tgtatattac aaaggctata
     2881 gattactttg accttgcaaa tgggtatggt tctctctttg aggcgttcag caattctggt
     2941 gatacaaagt ttcttgtaat atcttttact tcagactggc tctatccacc agttcagtcc
     3001 aaggagatag ttcgtgcgct ggaggcaaat ggtatagatg ttacttatag ggagatagag
     3061 tcaaattacg gacatgacgc atttttactt gaggaagatg aacagacccg tataataagt
     3121 agcttccttg agcatgttta tagagggata agatgaggag agactacgag attatagtgg
     3181 gtcttataga taaaaatgcc agggttctag acctgggctg tggaaatggt gagctgcttg
     3241 ctctgttaga gaaggagaag aatataaggg ggaaggggat agagataaac gggaaaaggg
     3301 tatctgaaag tatttcaaga gggatatctg tctatcaggg tgatatggaa gaagttctgc
     3361 ctgagtatcc ggataaatcg tatgactaca ttataataag tcagacactt caagagatta
     3421 gaaaccctga actggtaata actcattcac tgagggtatc cagattcgtt atcgttagcc
     3481 ttccaaactt tggatattgg aggataagac tatctatcct atttaaaggg cagatcccct
     3541 cttctatagg agactttgtc tacacctggt atgaaactcc ggacgttcat cccctcagta
     3601 taagagactt tgaagattac tgtaagataa aaggtataag aatagtcaag gcatattatc
     3661 tggctcacga caggatgatc agtatatgcc ctaacctctt tgccaccctg gggatatata
     3721 tgctgactca gtcttcgtag agttcggtag atagatatct ctcacctgta tcaggtagaa
     3781 ttaccacgat gagtttaccc tcgttttctt ctctctttgc tatctgaatt cctgcgtaaa
     3841 ctgctgctcc acttgatata cccgccaata ctccttcctc cttagctaat cttctggcgg
     3901 tctctatcgc tgattcatca cttactctta taacttcatc tatgatgtct ctattcagaa
     3961 cctctgggat gaatcctgca ccaatgccct gaatcttatg tttccccggt tttcctccgg
     4021 atagtaccgg agatttttca ggttcaacag ctatagccct gaacgatggt tttcttggct
     4081 ttataacttc tgctatccct gtaattgtcc ctccacttcc tactccggca accacaatat
     4141 ccactttgcc atccgtatct ctccatatct cttctgcagt cgtttttctg tgtatttcag
     4201 gattggctgg atttttgaat tgatatggaa taaaatagct aggattagta ccggctaatc
     4261 tctctgcctc ttctatagct ccttccatac ccttttctcc aggagtaaga atgatttctg
     4321 caccaaatgc cttcaataat tttctcctct cgatactcat agtgtcaggc attaccagta
     4381 tacatctata cccttttaat gcacataccc atgccaatgc tatcccggta tttccactgg
     4441 tgggttctat tattattgaa tctttattta tcttccccaa tctttctgct tcttcaatca
     4501 tactcacccc tattctatct tttatgctcc cagcggggtt gaagaattcc aattttgcca
     4561 ccaggctagc ccttatatct cctccaattt tattcagcct aactaacggg gtattcccta
     4621 tgaggtctct catactttca gctattttca tcaaagattc ctcctctgtc ttatggaaat
     4681 cttatcgatt atatcatatt tatatcaatt ttacctgaaa atacttgata tattttctac
     4741 aatctataaa attattataa ttacataatt aaaacggtaa gaataaaatt tggaggtgta
     4801 aactttaatg gaagtaccta ttggtaaagg tagatgggca acaaggtctt atggttttga
     4861 agagatatcc atagtaccag gggatataac cgttgaccct gaactggtag atgtttctct
     4921 ggagataggt gattttaaat ttgaaattcc gataattgca gcagctatgg atgggattgt
     4981 agacccaaac tttgctgaag tgatgggaaa acttggagga ttggcggtac tgaaccttca
     5041 gggaatccag accaggtatg aaaatcctaa agaagtactg gaggaaatcg tctctgctcc
     5101 tcctgaaaag gctactgaaa taatgcagaa gatttataag gaaccggtaa gggatgagct
     5161 ggtatataaa aggataaagg agatgaaaga ctcagggatt gttactgcct gttcttctat
     5221 cccccaggaa gcagagagaa tggtaaagat agctgtagag gcaggagttg atatctttgt
     5281 agtccagtct acggttatta ccgagagaca taaggctaag agttataccc cattaagtct
     5341 ggcaaaattc accagagaaa catctgttcc tgttgtggtt ggtaatgtgg taaacttcag
     5401 tgctgctaca gagttgtttg agacagggat aagcggtatt ctggttggag ttggtccagg
     5461 tggagcctgt actaccaggc aggtcctggg gataggtgtg ccacaggtaa ctgctattgc
     5521 agatgtggct tacgccaggg atatctttta ccatagaacc gggaaataca tacctgtgat
     5581 aggggacgga gggatgaata caggtggaga tatagctaaa gctattgcct gcggtgcaga
     5641 tggagtaatg ctggggcagg cactggctcg agctgaagaa gcctttggta gaggctatca
     5701 ctggggtatg gctatgagtc actctgctct cccgagggga acaagggtac atactgggac
     5761 aacaggaaca cttaaggaga tactctttgg tccggctaaa acggatgatg ggactcagaa
     5821 tcttgttggt gcattgaaga attctttagg ggtctgtggt gctctgaatc tcaaagagat
     5881 gcatcaggta gagattgtta tagctccttc tataaagact gaaggtaaga tacttcaaca
     5941 ggctcaaaaa gtgggaatgg gaaaataaat tattttagaa taccagtatt atatccagta
     6001 gcttctccac tctttaattt ttacgtgaag ttacatctct tacttttata atgctcctct
     6061 aagaagaaaa ggatattccc ctctccatct cagggagagg gggtaatctt agtaaggttt
     6121 tattgatgtc tgacaatttt gggaaaagct attgacaaaa atctttaata ctttatactg
     6181 ttaatattaa cagtatttag ctggaataat ttaatttgtg aggataacat taacagatgg
     6241 ctactataaa ggatgttgct gagaaagctg gagtatcggt aatgaccgtc tccagggtga
     6301 taaataataa taaatatgtg agccaagaga caagagaaaa agtagagcag gcgatggaag
     6361 aacttggtta tattcccaac gctctggctc atggattgat taccaagaag actcatgtac
     6421 taggcttgat agtctccgat attactaacc ctttctttac aacgatagct agaggagtag
     6481 aagatacagc tattaagaat ggatttaaca ctatactttg caattcagat gaagatgtag
     6541 agaaggaaga gagatatata gaacttttgc taagaaaaag agtagaggga attattctta
     6601 gccctgcaga ctgtgataag aggagaagaa tagaacagat aatatccaga aagatcccgc
     6661 tggttcttat agatagacgt atcagtggac tacagattga ccgtgtatat agtgatagta
     6721 taaatggagc ttatgagtta acaaaatatc taatagatct tgggcataga agaataggca
     6781 ttataatagg accaagaagg atatctacag cagtagatag agtggaagga tataaaagag
     6841 ctcttcagga agaggggatt tcaatggaag aagagctgat aagatgggga aagaaatata
     6901 gtagagaaga tggatattta aatgcaatag agttattaac tcttgagcac cctcctactg
     6961 cgatatttgg aggaaataga ctgataacgg tgggtatact tagagctatt agagagttga
     7021 agcttagaat tcctgatgat atttctgtgg tttcttttga tgaggtggaa gatatatcgg
     7081 taacaaatcc atttcttact gtgataagcc agaattcgta tgttatggga gtggtagcta
     7141 ccgaacagct tttagaaaga ataaggggga ataataaatc tccggaagac ctgcagaaaa
     7201 ttgtattaca gccaagatta gttataagaa agtcctgtgg gagcccaaag gaggaggtga
     7261 tgaaagaata gggctagatg gctgtggaga taaaagcaga ttatcgaaaa tattttatta
     7321 gaggaggtat taaaagatgg agaagttttt gaggattt
//
LOCUS       DAOTKU010000019         7310 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_9867_length_7310_cov_3.262026, whole genome shotgun sequence.
ACCESSION   DAOTKU010000019 DAOTKU010000000
VERSION     DAOTKU010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7310
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9867_length_7310_cov_3.262026"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="P5310_00950"
     CDS             <1..652
                     /locus_tag="P5310_00950"
                     /inference="COORDINATES: protein motif:HMM:NF014879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU31962.1"
                     /translation="ILLDSQGEKIFILDEKGSPITDEDLAYILAFLAIRTQPRKDVAF
                     PVYMPRVFEEGLRHYGGNVVWTKSSDRAIMETSTRNHIVFASDGEGGAIFPQFQFTYD
                     GMFGFAKLLEFLSIENIPLSQLKDLVPPYSLVKSRVSCPWEHKGRVMRELLEKKYDKE
                     VDYTEGIRVFTDDGWFLVLSDPNRPEFHIYAEGKAIREAERLAREATEMINNIIPK"
     gene            complement(733..3597)
                     /locus_tag="P5310_00955"
     CDS             complement(733..3597)
                     /locus_tag="P5310_00955"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HRU31963.1"
                     /translation="MLKEKKNLLINLLKLLITVYVLYFIFKKIPVDTLIASFINVRLR
                     YLGAAVLLGFVFTLIKIFKWHLLLRDLIPDISFRSSIDGYLSGMSLGIITPGRIGEVG
                     RISEIPKEKRLAGVGLVLWDKIFDLFIVVFLSLWGIWYFVNSGVAITAGVLLIALLFI
                     LFNTRYLNILLKLPVIKKYPQLLEGLNHLKRRTILTGLILTLFSYLIVIFEVLLLVRA
                     FGYNLGREIFISYPLVMLANLIPITVGGLGVREGIAILLLGRFGLPEELAFNSAFLIF
                     LINTALPGFCGLFPMNRDILKSKFVPVAITVIAGLVRFYNIGARSIWLDEGITVNLAW
                     DNIKNIILDRASTGIHPPLYFILTHFWIRIFGDSEVALRSFSAIFSVLSIPLIYKIAS
                     RIFDLPTAIIASLLFALSPFQIYYSQEARMYPLITFLFLLSLYLLYRWNEDRLKSDKK
                     FLIPVVILNVISLYVHIYSVFLIVLENIYIFFTNIRDWKRLKCWVTYQLVIVLLFSPW
                     IYVILKNRTPDVYQGKQSLTLSVIKNSFIEINLGYARGIFAERNLLQYIFYLFLALFI
                     IGLLPPYRDKKGFFLVALYTFIPFLLLILISFDKSFFSARYLSPFVAGYFILLARGIR
                     KFKFYPVVILILLLILGIDSLAIYNYNKKLDFISRPWRRVVEYIHSEVSDRTVALITA
                     PQMYRPFNYYNRDKIPYEKIDAFGNVAVDIYRGTYSYKNVWFVMAGEESSDPRGKIKS
                     WLDSKYKRLDMVEYYKLSAYLYEVPEEFNRVKVIFYSPDVDNIEFTVEIRYPESNTDN
                     IIETFDKLKLKGTFFFTADAALTHKKVIKSLLDKGYEIGMLGESYVDYTTYSEEEITE
                     SLKKTEEIIEGIAGKEINLFRPPRAAFDKNLLNVAYKLGYTLKFWSSDIRRWTHYEPE
                     AASEKLLKELSPGKIVNLGIWDNFSRSVLFSLLQVWHPEN"
     gene            complement(3557..5179)
                     /locus_tag="P5310_00960"
     CDS             complement(3557..5179)
                     /locus_tag="P5310_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU31964.1"
                     /translation="MNSVYLESMLKEIPRLLGLVDKNPSSPTFGCFDRQFWHYNVVDF
                     PCARFQEAVLTLAILYQNEFPDNAYYKNSNIFSLIDGALRFWSDMQDSDGSFSEWYPV
                     EHSFVATAFTTYAISETLLILGGDIPSYKKVLKSLRRAGEWLLNYTDFTACNQVAGAV
                     TALFNLYLLTSEERYRDGATRKLEQLKDRQNPEGWFPEYGGPDTGYLSLAIDYLSKYY
                     EKSEDPLAREMIDRALGFLVYFAHPNGTFGGEYGSRNTKYIIPAGVEFSSRWNKDAGV
                     IASLLRESLGKMQTVGPYNLDDRYLSYTGYTYLQAHMNMHQGNLEPPMFEMEFVKFLP
                     NSGILVVSNDNFYFISNLKKGGVFRLFNKKTNSSISDSGVIVEIGGKTYSSGWLNDKS
                     DLSFENYKAMTRNSLLRLSYKTMSSTDLVLSRLFQLIFGRFTGLSLKVKDILRRFLIT
                     YRGESNIEVTRYIDITGEEITVRDEITSDKDIEKITVGAENPYIYIPSSRYFEPQDLD
                     NCPKVIYPEKTRVKIVRRYDAEGKEELTYQST"
     gene            complement(5172..6116)
                     /locus_tag="P5310_00965"
     CDS             complement(5172..6116)
                     /locus_tag="P5310_00965"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRU31965.1"
                     /translation="MNRYDVSFVIPLKNEAESLETLYRGIVENTTPLGLSFEIIFIDD
                     GSTDNSFDIIKELHKRDSRVRGVRFRRNFGKSSALSAGFKLASGDIVITMDADLQDDP
                     VEIPRFIEMINKGYDVVSGWKKVRHDPPGKTLPSRLFNKLTSIATGVKIHDFNCGFKA
                     YRNEVVKSIEIYGELHRYIPALAYGKGFSVGEIVVTHHPRKYGRSKYGLERLFKGFLD
                     LFTVVFLTRFLRRPMHIFGTTGILLFIIGFIINLYLVIYKLLTGALIGNRPLLTLGVL
                     CMILGIQSLSTGLLGEMINNISYGQKEEYSILEEVGIE"
     gene            complement(6113..>6699)
                     /locus_tag="P5310_00970"
     CDS             complement(6113..>6699)
                     /locus_tag="P5310_00970"
                     /inference="COORDINATES: protein motif:HMM:NF019113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HRU31966.1"
                     /translation="MICPFMVRLGEPYREEGLRQLKIHIQYLMNPNVYLFHHAWDENT
                     LSLTPHYWARGNGWMLATLVEVIPYLQEEERVYFESIIRNMVETLFKIQDKSGLWHTL
                     INMKGTYLETSGSALLGYGINKAISLGIISEIYKDTVNRLFSGIKLRISKEGDVLGVS
                     EGTGPGDYIYYATRSTGIFPHGQGSVLLFLGGLK"
     assembly_gap    6700..6709
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6710..7246)
                     /locus_tag="P5310_00975"
     CDS             complement(<6710..7246)
                     /locus_tag="P5310_00975"
                     /inference="COORDINATES: protein motif:HMM:NF019113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HRU31967.1"
                     /translation="MRLEWAKGWDLTGVEELIKKAKNEIRMGKIRSAENCLDEAMSMM
                     GSIELRYNSPHINVKNIPPVLDNGELTRLIAIRTMDRGIGGYSWGQTVAMKGLLATGN
                     WEYVKAIIDGYIEKDMEPKNVNDCAIGDVLITLYELLGDERYIEKARRLGNFILSCKD
                     RLPSGGIVHEPGSGQLWID"
BASE COUNT         2286 a         1582 c         1297 g         2135 t
ORIGIN      
        1 aatactgctg gatagccagg gagaaaagat atttatactg gatgaaaagg gttctcccat
       61 aactgatgag gacctcgcct atatactggc atttcttgct ataaggacac agccgagaaa
      121 ggatgttgct tttcctgtat atatgccaag agtctttgaa gagggattga gacattatgg
      181 cggtaatgtg gtctggacaa aatcgtccga tagggcaata atggagacct cgaccaggaa
      241 tcatatagta tttgcaagtg atggtgaagg aggagccata ttccctcaat tccagtttac
      301 gtatgatggg atgtttggat ttgctaaatt acttgaattc ctttcaatag agaatatccc
      361 actttctcaa ttaaaagacc tggttccccc atattctctt gtaaagtcaa gagtaagttg
      421 tccttgggaa cacaaaggta gagtgatgag agagttgctc gagaaaaagt atgacaaaga
      481 ggttgactat actgagggga taagagtctt tactgatgat ggctggttcc tagttctatc
      541 tgacccaaat cgtccagaat ttcatatata tgccgaaggc aaagctataa gagaagctga
      601 aaggttagca agagaagcta ctgaaatgat aaataatatc ataccgaaat aagacctgct
      661 aagatagggt agattcacgc gtttggaagc tgagatactg aagttcaaaa agcccgctaa
      721 aaatgcgggc ttttaatttt cagggtgcca aacctgcaat agagaaaaga gtactgatcg
      781 ggaaaaatta tcccatatac caagattaac tattttccca ggagaaagct ctttcagaag
      841 cttttccgat gcagcctcag gttcataatg agtccatcgc ctgatatctg agctccagaa
      901 tttcaatgtg tatccaagct tatatgcaac atttaagaga tttttgtcaa atgcagcccg
      961 gggtggtctg aacagattta tctctttccc tgctataccc tctatgatct cttccgtctt
     1021 cttaaggctc tcagtgatct cttcttcaga ataagtggta taatctacat aagattctcc
     1081 cagcatacca atctcataac ccttatctaa cagagacttt ataacctttt tatgagtaag
     1141 ggcagcatca gcggtaaaaa agaaggtccc cttcagttta agtttatcaa atgtctctat
     1201 aatattatct gtattgctct cagggtaacg tatctctaca gtgaactcga tattatctac
     1261 atctggacta taaaatataa cttttaccct gttaaactcc tctggaactt catacagata
     1321 agcactgagc ttataatact ccaccatatc taatctttta tacttactat ctaaccagct
     1381 ctttatctta cctctggggt ctgaactttc ttctccagcc attacaaacc agacattttt
     1441 ataagaatag gttcccctgt atatatccac tgctacatta ccaaaggcat caatcttctc
     1501 gtatggaatc ttatccctgt tatagtagtt aaatggtcta tacatctgtg gggctgttat
     1561 cagagccact gtacgatctg aaacctcaga atgtatatat tcaaccactc ttctccaggg
     1621 acgggatata aagtctaatt ttttattgta attatatatc gccagagaat ctatcccgag
     1681 gataaggagc agtatgagga taacaaccgg gtagaacttg aactttctta tccctctggc
     1741 cagtaatata aaatatccag ctacaaaagg tgatagatat ctggcactga agaagctctt
     1801 atcaaaagag atgaggataa ggagaagaaa tggtataaat gtataaagcg caacgaggaa
     1861 gaaacccttc ttatctctat aaggtggaag aagtccaata atgaatagcg ccaggaaaag
     1921 atagaatatg tactgtagga ggtttctctc tgcaaagata ccacgggcat atcccagatt
     1981 tatctctata aaagaattct ttataacact taaggtaaga ctctgctttc cctggtacac
     2041 atctggagtc ctgttcttta atatcacata aatccagggg gaaaatagta acactataac
     2101 aagctgatag gttacccaac attttaatct cttccaatct ctgatatttg tgaagaatat
     2161 atagatattt tccaaaacga taagaaaaac agaatagatg tgcacataga gagaaatcac
     2221 attcaaaata actacaggga tcagaaattt cttatcagac ttcaatctgt cctcgttcca
     2281 cctgtaaagt agatatagag ataagaggaa cagaaatgta atcagcgggt acatcctggc
     2341 ttcctgagag tagtagattt gaaatggaga aagggcaaat aaaagactgg caattatagc
     2401 tgtgggaaga tcgaagatcc tggaagcaat cttatatatc agtggaatac tcaatacact
     2461 gaatatggca gagaaagacc tcaatgccac ctcagaatca ccaaagattc ttatccagaa
     2521 atgggtgaga ataaagtaga gaggtgggtg tatgccagta gaagccctgt ctagaatgat
     2581 attttttata ttgtcccagg caagattgac ggtaattccc tcatctaacc agatgctcct
     2641 tgcacctata ttataaaatc tgaccagtcc ggcaattaca gttatcgcta caggaacgaa
     2701 cttacttttg agtatatctc tgttcatagg gaacaaccca caaaatccag gtaaagcggt
     2761 attgatcaga aatattaaaa atgcagaatt gaatgccaat tcctcgggca gtccaaacct
     2821 acccaacagc aggatagcga taccctctct gaccccaagt ccgcccacgg ttatgggtat
     2881 aagattagcc agcataacca gtgggtacga tataaaaatc tcccgtccca ggttataacc
     2941 aaaggctctt actaggagta gaacctcaaa aataacaatt aaatagctga agagagtaag
     3001 gattaaaccg gtaaggatcg tccttctctt cagatgattt aacccttcca ggagctgcgg
     3061 atacttttta ataacaggta gcttgagaag aatatttaaa tatcttgtat tgaagaggat
     3121 aaacaggaga gctataagta atacccccgc agttatagct acaccggagt taacaaagta
     3181 ccagattccc cagagtgata gaaaaactac tataaaaagg tcaaagatct tatcccataa
     3241 tactagacca acaccagcaa gtcttttctc ttttggtatc tccgatatac gtccaacctc
     3301 gccaattctt ccaggagtta ttatccccag agacattcca gagaggtatc catcaataga
     3361 ggaacgaaaa gagatatcgg ggattaagtc tctcagaaga aggtgccact taaatatttt
     3421 gatcagggta aatacaaaac ccagtaagac agctgcccca agatatctta acctgacgtt
     3481 tataaaagaa gctattagag tatcaactgg tatcttcttg aagataaaat agaggacata
     3541 tactgtaatt aacagcttaa gtagattgat aagtaagttc ttcttttcct tcagcatcgt
     3601 atcttcttac tatttttact ctggtttttt ctggatatat gactttaggg caattatcca
     3661 gatcctgagg ttcaaagtat ctggaggatg gtatatagat atagggattc tctgctccaa
     3721 ccgtgatttt ttcaatatcc ttatctgaag taatctcatc cctgacagta atctcctcac
     3781 cggttatatc tatatacctg gtaacttcaa tattactctc acccctgtat gttataagaa
     3841 atcttctaag gatatccttt accttcaggc tcagaccggt aaatcttcca aatataagct
     3901 gaaatagtct ggacagaacc aggtctgtag aagacatggt cttatatgac aacctgagca
     3961 gactattcct ggtcattgcc ttataatttt caaagctcag atcactctta tcatttagcc
     4021 atccgctgga ataagtcttg cctcctattt ccactataac accactgtca gagatcgaag
     4081 agttggtctt tttattgaag agtctgaaga ctccaccctt tttcaggtta gagataaaat
     4141 agaaattatc attactgaca acaagtatcc ctgagtttgg aagaaactta acaaattcca
     4201 tttcaaacat aggtggctcc aggtttccct ggtgcatatt catatgtgcc tgaagataag
     4261 tgtatcctgt gtaagagaga tatctatcat ccagattata cggtcccaca gtttgcatct
     4321 tcccgaggga ttctctcaat agagaagcta tgaccccggc atccttattc cacctgcttg
     4381 aaaattctac accggcaggg attatatact ttgtatttct tgaaccatat tctccaccaa
     4441 atgtcccatt tggatgggca aaatagacaa gaaaacccag agctctatct atcatctctc
     4501 tggctaatgg atcttcagat ttttcatagt acttgctgag ataatctata gcaagtgata
     4561 gatagcctgt atcaggacct ccatattcag gaaaccatcc ctccgggttc tgtctatctt
     4621 tcaactgttc taactttctg gtagctccat ctctgtatct ctcttcactg gtaagcagat
     4681 agaggttaaa aagggcagtc acagctcctg ccacctgatt acaggctgta aaatcggtat
     4741 aatttaataa ccactcccct gcccttctga ggcttttcag gaccttctta taagaaggaa
     4801 tatccccacc aaggatcagg agtgtctcgg atatggcata ggtggtgaag gctgtagcca
     4861 caaaactatg ttcaacaggg taccactcgc tgaaagaccc atcactatcc tgcatatcag
     4921 accagaatct caatgctcca tctattaagg agaatatatt agaattttta tagtatgcat
     4981 tatcgggaaa ttcattctga tacagtatag ccagagtaag gaccgcctcc tggaatctag
     5041 cacaggggaa atccaccaca ttgtaatgcc agaactgcct gtcgaaacac ccaaatgttg
     5101 ggctactggg attcttatca accaatccca gtaatctcgg aatctctttc agcatagatt
     5161 ctagatatac gctattcaat gccaacctct tctagtatgg aatactcctc tttctgtcca
     5221 taagagatat tattaatcat ctctccgagc aaccctgtag agagagactg aatcccaagt
     5281 atcatacaaa ggacacccag agtaagaaga ggtctattac ctataagggc acccgttaac
     5341 aacttataaa tcaccagata gaggtttata ataaatccaa taataaagag gagaatacct
     5401 gtcgttccaa agatatgcat gggtcttctt agaaatctgg tcaggaatac aactgtaaac
     5461 aaatccagaa aacccttaaa aagcctttcc aaaccatact ttgatcttcc atactttctg
     5521 ggatgatgag tcacaactat ctctccaact gaaaacccct tgccataagc taatgcaggg
     5581 atatatctgt gaagctcacc atatatctct atagatttga ccacttcatt tctgtaagcc
     5641 ttaaatccgc agttaaaatc atgaatcttt actccggtag ctatagatgt aagtttatta
     5701 aaaagtctgg atggcaaggt cttgcctgga gggtcatgac ggaccttctt ccagccagag
     5761 acaacatcgt atcctttatt tatcatctct ataaatctgg gaatttctac agggtcatcc
     5821 tgcagatctg catccatagt gataactata tccccactgg ccagtttaaa accggctgat
     5881 aatgcacttg acttaccaaa gttcctcctg aaccttaccc ctctcaccct gctatccctt
     5941 ttatgtaact cttttattat atcaaaagaa ttgtctgtac tgccatcatc gataaatatt
     6001 atctcaaaag ataaccctaa aggagtcgta ttctctacta tacctctata cagggtttcg
     6061 agagattcag cctcattctt taatgggata acaaatgata catcatacct attcatttta
     6121 aacctcctaa aaagagaaga acagagccct gtccatgggg aaagatcccg gtacttcttg
     6181 tggcatagta tatataatct ccaggacctg tcccttccga tacacccagc acatctcctt
     6241 ctttactgat cctgagtttt ataccggaaa agagcctgtt aactgtatct ttataaattt
     6301 cacttataat tccaagagag atagctttat tgatcccata tcccagaagg gcactccccg
     6361 aagtctcaag atatgtacct ttcatattta taagtgtatg ccataaacca gatttatcct
     6421 gaattttgaa cagggtctca accatatttc ttatgataga ctcaaaatac accctctctt
     6481 cctcctgtaa ataaggaata acctccacaa gagtagccag catccagccg ttgcctcttg
     6541 cccagtagtg tggagtcaga gaaagagtat tttcatccca ggcatggtgg aacagatata
     6601 cattgggatt cataaggtat tgtatatgta tctttaactg tctcaaaccc tcttctctgt
     6661 agggttctcc taatcttacc ataaatgggc atatcatatn nnnnnnnnna tcaatccata
     6721 actgtccact tccaggttca tggacaattc cacctgaagg gagtctatcc ttacaggata
     6781 gaataaagtt acccagtctt ctcgcctttt caatatatct ttcatctccc aagagctcat
     6841 agagagttat caaaacatct cctatggcac aatcattcac atttttaggc tccatatcct
     6901 tttctatata tccatctatt atcgccttta cgtattccca attaccggtg gcgagtaatc
     6961 ccttcattgc aacggtctga ccccaggagt aaccacctat tcctctatcc atagtcctga
     7021 tagcaataag cctagttaac tcaccattat ccagtacagg tggtatattc ttcacattta
     7081 tatgcggaga attgtatctt agttcgatcg accccatcat actcatagcc tcatccagac
     7141 aattctcagc cgaacggatc ttgcccatcc ttatctcatt cttcgccttc tttataagtt
     7201 cttccacccc tgtaaggtcc cagcccttag cccactctaa tctcatactg tgaaatttat
     7261 cttcaaccaa taaaagtttt ccatctaaag tttcagctac agattgttgt
//
LOCUS       DAOTKU010000020         7253 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_9947_length_7253_cov_3.106974, whole genome shotgun sequence.
ACCESSION   DAOTKU010000020 DAOTKU010000000
VERSION     DAOTKU010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7253
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9947_length_7253_cov_3.106974"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /gene="thrC"
                     /locus_tag="P5310_00980"
     CDS             <1..639
                     /gene="thrC"
                     /locus_tag="P5310_00980"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012513370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HRU31968.1"
                     /translation="ALNLVREAGRNYPITIVNSINPYRIEGQKTASFEVCDALGDAPD
                     YLFIPVGNAGNITAYWKGFKEYKQIGKSQKLPRMMGFQSEGAAPIVRGYPIDNPETIA
                     TAIRIGNPASWNSAVQAAKESGGIIDMVSDDEILYAYKFVASMEGVFVEPASAASIAG
                     LLKYTREGRLEKDSVIICVLTGHGLKDPDTAVKIAPSPTVISPSIEELYQYL"
     gene            636..1538
                     /gene="thrB"
                     /locus_tag="P5310_00985"
     CDS             636..1538
                     /gene="thrB"
                     /locus_tag="P5310_00985"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HRU31969.1"
                     /translation="MRLVARVPATTANLGVGFDTLGLALKLYNTFEVSDSEPDFLEIN
                     GEGMECLYNRENNIFLKALDFFYKLYNLTPPSLSIKISINIPISKGLGSSASAIVAGL
                     MIANSLLDKGLSKEELLKYAVKLEGHPDNVSPAFFGGCTISLVSDGKVMVKKVPLPDD
                     LSIIVAVPYFDVPTEPSRKILPDYVPLDVTVKGLARIGMFLVSLITDNLKDLPVILED
                     ELHEPYRARFIPNYNGIKKELLSMGALGVMISGSGPSMVILTERERVEELVESVGFVL
                     TKNNIIAKILNLDIDYEGAVLEVN"
     gene            1539..2555
                     /locus_tag="P5310_00990"
     CDS             1539..2555
                     /locus_tag="P5310_00990"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HRU31970.1"
                     /translation="MYNVAVVGATGLVGREMVSILETRNFPVKSLRLLATSRSQGAII
                     QFKGQDIVVEEATPDSFEGIQIALFSAGEKGSKELAPYAVKAGAVAIDNSNAFRMEPD
                     VPLVVPEVNEEKIREHKGIIANPNCSTIQMVVALAPLHRRFKIKRIVVSTYQSVSGTG
                     REAVDELWEETRSIIEGKQITPKVYPYQIAFNLLPHIDIFYPNGFSKEEMKMLNETRK
                     ILDDQSIKVCATTVRVPVFRAHSESVNIETELPMTPEEVREILSKSPGVVVVDNPEER
                     LYPMPIMAEGRDEVFVGRIRKDESVENGIVMWVVSDNIRKGAALNAVQIAESLVKLGL
                     IEEV"
     gene            2558..>2691
                     /locus_tag="P5310_00995"
     CDS             2558..>2691
                     /locus_tag="P5310_00995"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="catalyzes the formation of 4-phospho-L-aspartate
                     from L-aspartate and ATP, in Bacillus, lysine sensitive;
                     regulated by response to starvation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HRU31971.1"
                     /translation="MLLVHKYGGTSVGTPERIKNVAGKLARLKREGNDLVIVVSAMGD
                     T"
     assembly_gap    2692..2791
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2792..3643
                     /locus_tag="P5310_01000"
     CDS             <2792..3643
                     /locus_tag="P5310_01000"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HRU31972.1"
                     /translation="ELKKGKVIVVAGFQGICDQNDITTLGRGGSDTTAVALGIALGVE
                     KIEIFTDVKGVYTADPRLVKNARKLDVISYDEMLELASQGARVMHTRAVELGWIYKMK
                     IEVKSSFEDCPGTIIQEVSEMEPVSRVRGIAHKKDIAKITLKRVLDRPGVAHRIFKPL
                     GDAGINVDDIIQNVSEFGYTDISFTIPIDDTDKALQILEKVKEELDIKEVLCNPAVGK
                     VSIVGVGIQSTPGIAAKMFGLLGEAGINIDMISTSEIKIGCIINEEDVERAIKVLHDG
                     FGLGENS"
     gene            3640..3909
                     /locus_tag="P5310_01005"
     CDS             3640..3909
                     /locus_tag="P5310_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010871082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HRU31973.1"
                     /translation="MKRAIITVTGVDHTGIVAGISSVLADFNVNILDITQTIMGDIFV
                     MALLVDISGLEDITPLVNKLSEEGDRLKVKVSFEREEIFRTMHRI"
     gene            3913..5271
                     /locus_tag="P5310_01010"
     CDS             3913..5271
                     /locus_tag="P5310_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HRU31974.1"
                     /translation="MIFTTEEILETVRMIQLDHLDIRTITLGIDLLDCRRDSVKETVR
                     LLSEKLKLATDGFLDTVNRVGVEYGIPIVNKRVAITPVSLLLSSTDMEEYIYLAEKLD
                     GVAKDIGLDFIGGYTCFLEYEVSQSDRALLKSIPAVLNETERLCSSIVPATTKAGINL
                     DAIREVVPIILETARLKNNPSVCAKLIVICNPPPDNPFMAGAFHGIKRGNRVLNVGIS
                     GPGAIRSALEKLPENSDILSVVEEIKRISFKITRAGEFIGKEIANRMGIELVSVDTSL
                     APTPEEGDSIARIIERIGVERFGAPGTTGLLFLLNDALKKGGLMASSLVGGLSGSFIP
                     VSEDKGIAEAVSSGAINLEKLESLTNVCSVGLDMIPIPGDTSPYTLMGIILDEMAIGV
                     MSNKTTAVRIIPVPGKRAGEWLELGGLLGRAPIMDVNRFSCKALFERGGRIPPPISSY
                     RN"
     gene            5481..6266
                     /locus_tag="P5310_01015"
     CDS             5481..6266
                     /locus_tag="P5310_01015"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HRU31975.1"
                     /translation="MKLSCQENLVNGDSLRGNVEKLESFGFDGVELWGSNLRDRLDEV
                     KSAFKGRQIRISTICSGYSGCLLDPNKDERDRAVRDIKELLSLSMELGAVGLIVVPVF
                     GPPRLPDLTPLMNVIDLEKKLLVELLNELGEDAYSKGVCVLVEPLNRYETHFVKTLDF
                     GKEIINHTHKKGVRLMADFFHMSIEESDISRAISENMDLIAHIHLADSNRLLPGMGHT
                     DFSAPFKVLKEKGYKDYMALECGIPGDPDKLLPEAVKFLRSLM"
     gene            6276..7247
                     /locus_tag="P5310_01020"
     CDS             6276..7247
                     /locus_tag="P5310_01020"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRU31976.1"
                     /translation="MSLRVGFIGTGNIARTHMTQLSQMPDVKIVCGYDPSTEAISLSS
                     ERFGFTPYDSLDKMLDSEKLDCAYICIPPFAHGESEKKLIERGIPFFVEKPIGLDLST
                     VKEIASLIESKGLIVSAGYQWRYLDIVKKAKELVSPDKIGMVLGYWMGGLPMVHWWRI
                     REQSGGQFIEQTTHIVDLARFIIGDIDEVYALFARRIIFDVENMDVPDVGTVNLKFKN
                     GVIGNISNTCILNFGYTVGLHIYERDLVVEILSNKLVINERKKRTEERSDNNPYFDED
                     EAFLNAVKENDPSMILSTYRDGARTLAVTISAWESFVSGKPVKVEEV"
BASE COUNT         2194 a         1115 c         1825 g         2019 t
ORIGIN      
        1 gcccttaacc tggtaagaga ggctggaaga aattatccaa tcaccatagt taactcgata
       61 aatccttata gaatcgaggg acagaagaca gcttcttttg aggtctgcga tgctctggga
      121 gatgctcctg attatctctt tataccagtt ggcaatgctg gaaatatcac cgcctactgg
      181 aagggattca aagagtataa acagataggg aagagccaga aattaccgag gatgatgggt
      241 tttcaatctg agggtgctgc accgatagtg aggggttatc ctatagataa tccagagaca
      301 atagctactg ctataagaat aggaaatcct gctagctgga attctgctgt ccaggctgct
      361 aaagagtctg gtggaattat agatatggtc tcagacgatg agattctcta tgcctataag
      421 ttcgtagcca gtatggaggg agtatttgtt gaaccggctt cagctgcatc tattgccgga
      481 ttacttaaat ataccagaga aggaagactg gagaaagata gcgtgattat ttgtgttctt
      541 actggacatg gcttgaaaga cccagatact gccgtgaaga ttgctccttc tcccacagtt
      601 atctccccta gtatagagga gttgtatcaa tatctatgag attagtagcc agggttcctg
      661 ctactacagc taatttaggc gttggttttg ataccctggg attagcgtta aaactttata
      721 ataccttcga agtgagtgat tcagaaccag attttttaga gattaatggt gaagggatgg
      781 aatgtcttta taacagagag aataatatat ttctaaaggc actggatttc ttttataagt
      841 tatacaatct tactccgccg tcgttaagta taaagatatc tattaatatc cctataagta
      901 aaggtctcgg aagtagcgca tctgctatag tagccggatt gatgattgca aatagtctcc
      961 tcgataaggg actttcaaag gaagaactgc tgaaatatgc agtaaaattg gaaggacatc
     1021 ctgataatgt gagtccagca ttttttggtg gatgtactat ctctctagta tctgatggaa
     1081 aggttatggt gaaaaaggtc cctttacccg atgatttatc tatcatagta gctgtcccat
     1141 attttgatgt gcctactgaa ccttccagaa agatattacc cgattatgtc ccgcttgatg
     1201 ttacggtgaa aggacttgca aggataggta tgtttttggt atccttaata actgataact
     1261 tgaaggattt acctgttatc cttgaagatg agctccatga gccgtacagg gctcgtttta
     1321 taccaaatta taatggaata aaaaaagaat tgctctctat gggggcttta ggtgttatga
     1381 taagcggatc tggtccatct atggtcattc ttacagagag agaaagggtt gaagagttag
     1441 ttgaatcggt aggttttgtg cttactaaaa ataatattat agctaagatt cttaatcttg
     1501 atatagatta tgaaggtgca gtattggagg tgaactagat gtataatgtg gcagtagttg
     1561 gggccactgg gctcgtgggt agagagatgg tctcaatcct tgaaaccagg aattttcctg
     1621 ttaaatcact cagacttttg gctacgagta gatctcaggg tgctattatt cagtttaaag
     1681 ggcaggatat agttgtggaa gaagctaccc cagatagttt tgaagggatt cagattgcac
     1741 ttttttctgc tggagagaag ggaagtaaag aacttgcccc gtatgcggta aaggctgggg
     1801 cggtggcaat agataatagt aatgcgttta gaatggaacc ggatgtccct cttgttgtcc
     1861 cagaggtgaa cgaagagaag ataagagagc ataaggggat tattgcaaat cctaactgct
     1921 ctaccataca gatggtagta gcgttagctc cgctacacag gaggtttaag ataaagagga
     1981 tagtggtttc tacttaccag tctgtatcag gtaccggcag agaagcggtg gatgagctct
     2041 gggaggagac caggagtata attgagggga agcagataac ccccaaggta tacccttatc
     2101 agatagcatt taacctttta ccacatatag atatattcta tccgaatgga ttttctaaag
     2161 aagagatgaa gatgcttaat gaaacaagga agatccttga cgaccaatcg attaaggtat
     2221 gtgccaccac tgttagagtt cctgtgttca gagcccattc tgaaagtgta aatatagaga
     2281 ccgaactacc gatgaccccc gaagaggtca gggagattct gagtaaatcc ccaggggtag
     2341 tggtagtgga taatcctgaa gagagactgt atccaatgcc aataatggct gagggtaggg
     2401 atgaagtctt tgtcggtaga ataagaaaag atgaatcagt ggagaatggg atagttatgt
     2461 gggtcgtttc tgataatata agaaagggag cagcacttaa tgcggttcag atagcggaat
     2521 ctctggtgaa actgggcttg atcgaggagg tttagacatg cttcttgtgc ataagtatgg
     2581 tggtacctca gttggaacgc ctgagaggat aaagaatgta gcaggtaaac tggcaagatt
     2641 aaaaagagag ggaaatgacc ttgtgatagt ggtatcagct atgggtgata cnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngaactgaag aagggtaagg ttattgtggt
     2821 ggctggattc caggggatat gtgaccagaa tgatataaca acgcttggta gaggtggttc
     2881 tgatacgact gcagtagctc ttggtatagc tcttggagtg gagaaaatag agatctttac
     2941 cgatgtaaag ggtgtctata cagctgaccc cagactggta aaaaatgcca gaaaacttga
     3001 tgtaatttct tatgatgaga tgcttgagct tgccagtcag ggtgccaggg ttatgcatac
     3061 cagagcggta gaacttggct ggatatacaa gatgaagata gaggtaaagt cttcttttga
     3121 agattgtcca ggtactatta tacaggaggt aagcgagatg gaaccggtaa gcagagttag
     3181 aggaatagct cataaaaaag atatagcaaa gattacatta aagcgtgttt tggatagacc
     3241 tggtgtagcg cataggatat ttaagcccct gggggatgct ggtataaacg ttgacgacat
     3301 aatacagaat gtgagtgagt ttggttacac tgatatctct tttaccattc ctatagatga
     3361 tacagacaag gctctacaga tactggagaa ggtaaaagag gaactggata taaaagaggt
     3421 cttatgcaat ccagccgtag gtaaggtctc tatagttggg gttggtatac agagcacccc
     3481 tggaatagcc gctaagatgt ttggactttt gggagaagcg ggtataaata tagatatgat
     3541 atccacctca gagataaaaa ttggatgtat aataaatgag gaggatgtgg agagagcgat
     3601 taaggtatta catgatggtt ttggtttggg agagaatagt tgaagagagc tattattact
     3661 gtaacagggg ttgaccatac tggaatagtt gctggtataa gttcggtact ggctgatttt
     3721 aatgttaata tactggatat aacacaaaca attatgggag atatctttgt tatggctctt
     3781 ctggtggata tttctgggtt ggaggatata actcctctgg tgaataagct ctcagaggaa
     3841 ggggatagac ttaaggtgaa ggtctccttc gaaagggaag agatcttcag aacgatgcac
     3901 aggatataag atatgatatt cactaccgag gaaatcttag aaacagtcag aatgattcag
     3961 ttagaccacc tggatataag gacaattact ttgggtattg accttctgga ctgcaggagg
     4021 gatagtgtaa aggaaacggt aagacttctc tctgagaaat taaaattagc cactgatggg
     4081 tttttagata ctgtaaatag agttggggtt gagtacggga tacctatagt taataagaga
     4141 gtagctataa ctcctgtttc attattatta tccagcacag atatggaaga gtatatatac
     4201 cttgcagaaa aactggacgg tgtagcaaag gatataggtt tggattttat cggtggttac
     4261 acctgttttc tggaatatga agtctctcag tcagatagag ctctattgaa atctatccct
     4321 gcagttttga atgaaacaga acgtttatgt tcctctatcg ttccagccac aactaaggcg
     4381 ggtataaacc tggatgcgat aagagaggta gttccgataa tattggagac agctagactg
     4441 aagaataatc caagtgtatg cgcaaagctt atagttatct gtaatcctcc ccctgataat
     4501 ccttttatgg ctggagcatt tcatggtata aagagaggaa acagggtatt gaatgttggt
     4561 ataagcggac ctggagcaat aaggtctgct ctggagaaat tacctgagaa ttctgatata
     4621 ctttcggtgg tggaggagat aaagagaatc tcctttaaga taacaagggc tggagaattt
     4681 ataggtaagg agatagcaaa tagaatgggg atagaactgg taagtgtaga tacatccctt
     4741 gctccaactc cagaagaagg cgacagcata gccagaatta ttgagcgaat aggcgtggaa
     4801 cgctttggtg ctcctggaac taccggatta ttattcctcc tcaatgatgc cctgaaaaag
     4861 ggtggattga tggcttcctc tctcgttggt ggtttaagtg gaagttttat cccggtaagt
     4921 gaagataagg ggatagcaga ggctgtcagt tcaggtgcca ttaatctaga aaaactggaa
     4981 tctcttacca atgtatgctc tgttggactt gatatgatac cgataccggg agatacatcc
     5041 ccatatacat taatgggaat aatacttgat gagatggcga taggtgttat gtcgaataag
     5101 accactgcgg ttcgcatcat cccggtccct ggtaaaaggg caggggaatg gttggaactc
     5161 ggaggtctcc ttggcagggc tccaattatg gatgtcaata gattttcctg taaggctctc
     5221 tttgagagag gggggaggat tcctcctccg atcagtagct atagaaactg aaaatataga
     5281 tctatcgata agcagattct tgataaataa agactttctc aattgtgaac tgactcttct
     5341 aattccttgt aacattatct cagagccatt tacctgtgac aatatcacaa aacgataaca
     5401 attgcttgta agcttgggaa gaaaagtata aaatgaaaga ggtttcaatt atgattgtct
     5461 tcggaggagg tgtaaaaaat atgaaattat catgccagga gaacctggtg aatggagact
     5521 ctctaagagg gaatgttgag aagttagaat cctttggttt tgatggggta gagttatggg
     5581 gaagtaattt aagggataga ctggacgagg taaaatctgc ttttaaagga agacagatca
     5641 ggataagtac tatatgctct ggttatagtg gctgtttact tgacccaaat aaagatgaga
     5701 gagatagggc ggtaagggat ataaaggagt tattatccct ttcaatggag cttggggcgg
     5761 taggacttat agttgtacct gtatttggtc cacccaggtt gccagacttg accccgctta
     5821 tgaatgtaat agatctggaa aagaagcttc tggttgaact tcttaacgaa ctgggagaag
     5881 atgcctattc caagggtgta tgtgtgttag tagagccttt gaacagatat gagacacact
     5941 ttgtaaaaac actggatttt ggaaaagaga ttataaatca tacacataaa aagggagtaa
     6001 ggcttatggc tgactttttc catatgagca tagaggagtc agatatatcc agagcaatat
     6061 ctgagaatat ggatctgata gcacatatac atcttgccga tagcaataga cttctccccg
     6121 gtatgggaca caccgatttc tccgctccct ttaaggtgct gaaagaaaag gggtacaaag
     6181 attatatggc tctggagtgt ggaatacctg gagaccctga taaactgctt cccgaggcag
     6241 ttaaattctt acggtccctg atgtaggagg gaaagatgtc tttaagagtt ggttttatag
     6301 gtactggtaa tatagctcgg acccatatga ctcagcttag tcagatgcct gatgtaaaga
     6361 ttgtctgtgg atatgatcca tctactgaag ctatatctct gtcttcagag aggtttggct
     6421 ttactccgta tgatagcctg gacaaaatgt tagatagtga gaaattagat tgtgcatata
     6481 tctgtatccc accatttgcc catggagaat ctgaaaagaa acttatagag aggggtattc
     6541 ccttctttgt agaaaaaccg ataggtctgg acttatctac tgtgaaagag atagcatcat
     6601 tgatagaatc aaaaggtctg atagtcagtg ctggttatca gtggagatat ctggatattg
     6661 taaaaaaagc taaagagctt gtatctccag ataagattgg gatggtttta ggttactgga
     6721 tgggtggttt gcctatggtc cactggtgga ggataagaga gcaatctgga ggacaattta
     6781 tagagcaaac aacccacata gttgaccttg ccagatttat tattggtgat atagatgagg
     6841 tctatgccct ctttgccaga agaatcatct ttgatgtgga gaatatggat gttcctgatg
     6901 tagggacggt aaatctgaag tttaaaaatg gagttatagg caacatatcc aatacctgta
     6961 tactgaactt tggttatact gtaggacttc atatctacga gagggatctg gtggtggaga
     7021 ttctcagtaa taagctggtg ataaacgagc gtaagaagag gacggaggag agatcagata
     7081 ataatccata ttttgatgag gatgaggctt tcttaaatgc agtgaaagag aatgacccat
     7141 cgatgatact atctacctat agggatggag caaggacatt ggcggttaca atatcagcct
     7201 gggagtcatt tgtgtccgga aagcctgtaa aagtggaaga agtttaaaga gta
//
LOCUS       DAOTKU010000021         7203 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_10022_length_7203_cov_3.524622, whole genome shotgun sequence.
ACCESSION   DAOTKU010000021 DAOTKU010000000
VERSION     DAOTKU010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7203
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10022_length_7203_cov_3.524622"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /locus_tag="P5310_01025"
     CDS             complement(<1..557)
                     /locus_tag="P5310_01025"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HRU31977.1"
                     /translation="MFQRVISALIGIPVILALIRLGSISLQVFLLAIFIIGFFEYSRL
                     TESMGIKVRFWEGLIGGIFILLWSWEYATSVLETLIVLWVYLMLYFIIMQNIKDSLSI
                     ISTTIFGLIYLAVPLSMFLIMRKFDPSGYWCTMVMVGTWAFDSTAYFVGRKFGRHKLA
                     SILSPLKSWEGFIGGLLATVISSFL"
     gene            complement(564..1271)
                     /locus_tag="P5310_01030"
     CDS             complement(564..1271)
                     /locus_tag="P5310_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004632423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HRU31978.1"
                     /translation="MGSENLPVHVAIIMDGNGRWAQARGLSRISGHQEGKRNVKRIVK
                     YAANLGIRYLTLFAFSTENWRRPKEEVNGLMEILRLGLQEERPEFIREGIKVVFFGDR
                     SRLSEEVIREMELTEEATKFNDKLSLAFAINYSGRDDIVRAVRRIAIKIASKEINIED
                     INDRLISQELDTRDFPDPDLVIRTSGELRISNFLLWQSAYSEFYFTPLFWPDFDEKEF
                     DSAIEAYTHRERRFGGI"
     gene            complement(1276..1836)
                     /gene="frr"
                     /locus_tag="P5310_01035"
     CDS             complement(1276..1836)
                     /gene="frr"
                     /locus_tag="P5310_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013788073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HRU31979.1"
                     /translation="MDEKAFTRGIEEKMKQSIAVFQKELRGIRTGRANPALVEELIVD
                     YYGTSTPLFQLAKITIPDSRTILIQPWDKQSMHSIEKAILKSNLSLTPVNDGTSIRLN
                     IPSPTEERRKELVKLVKKKGEEAKVIIRNIRRTGIEELEKEKKNSKISEDLEKRLKDQ
                     LQKITDKYTEEIDKVLLEKEKEIMEV"
     gene            complement(1842..2558)
                     /gene="pyrH"
                     /locus_tag="P5310_01040"
     CDS             complement(1842..2558)
                     /gene="pyrH"
                     /locus_tag="P5310_01040"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010880427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HRU31980.1"
                     /translation="MRDIIRYKRILLKLSGEAFKEDSDPIGYQSLVNIAEEVQSIYRL
                     GVEIAIVVGGGNVIRGITSSSLSIDRATADYMGMLSTIINALALQSRLESMGIPTRVQ
                     TSIEMRQVAEPYIRRRAIRHLEKGRVVIFGGGTGNPFFSTDTAAALRAAEINAEVMIK
                     GTRVDGIYDSDPVKNPNATKYVEIKYMDILSKELKVMDATAVSLCMENKIPIIVFNIS
                     EQGNLKRLVCGEEIGTLVKE"
     gene            complement(2558..3154)
                     /gene="tsf"
                     /locus_tag="P5310_01045"
     CDS             complement(2558..3154)
                     /gene="tsf"
                     /locus_tag="P5310_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HRU31981.1"
                     /translation="MSINMDLIRELREKTGAGVMDCKKALISANGDVEKAVEILRKEG
                     IAVAEKKGARLAKDGRIESYIHPGNKLGVMVEVNCETDFVARTDEFKTLAKEIAMHIA
                     ASNPRYISIADIPEDVLESEKDIYATQAQNEGKPPQVIERIVQGKLEKFYQEVCLLEQ
                     PWIRDPEKKIKDIINDAIARLGENILIRRFVRFELGKD"
     gene            complement(3151..3942)
                     /gene="rpsB"
                     /locus_tag="P5310_01050"
     CDS             complement(3151..3942)
                     /gene="rpsB"
                     /locus_tag="P5310_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018963065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HRU31982.1"
                     /translation="MAIVTIKQLLEAGVHFGHQTKRWNPKMKPFIFAERNGIHIIDLQ
                     KTVVCMEKAYEVARDISGNGGKVLFVGTKKQAYEVVKTEAERCGMPYVNQRWLGGLLT
                     NFQTVRRGIEKLKELRSARDSGLWDSYTKRERKHLEIELKRLEKYVGGIENLDRPPDL
                     LFVIDTKKEENAVREAKRLGIPVIGVVDTNGDPEIIDYPIPGNDDAIRAIRLYCAKMA
                     DAVLEGTGMRKEEVEEEVVMPDIEELPADAIEEKVASEYEEELTE"
     gene            complement(4048..5712)
                     /locus_tag="P5310_01055"
     CDS             complement(4048..5712)
                     /locus_tag="P5310_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HRU31983.1"
                     /translation="MEEFPLTPEDLGLLLGGLSLFLYGIFQMSSGLEKAAGSRLRSIF
                     EKISASPWRGLLIGVVVTSIVQSSSAVTVLVVGFVNAGLLTLEGSLGIIFGANIGTTI
                     TAQLVAFKLTVIAPYFLLIGFLLVFAGKRKYIKYIGEAVFGFGMLFLGLNLMDSSLIT
                     LRNWAPFSNAMINMGKNPILGIITGAAVTAIIQSSSVTTSTVVALASKGAIPLNSAIP
                     IILGANIGTCVTALLASIGTSLSAKRTALAHLLFNILGVILIFPFLNPFIRLVSLSAD
                     DVARQVANAHTLFNVIWTFIWIWFTRQYASLIRKIIPGEERVFQRSTLILNPILLNTP
                     STALESAIKELVRMTDLVKEMYMMAFDDLEKNSLNYYKDILTMEDITDEIKASLIKYL
                     TRISATSLSEDEARELNAILRTVDDVERIADHITNIMEKIELKISDRVEFTEYAKSDL
                     KELKELILENMEDSFEMIRESDISNLEIISEREEEIDQKVKESKNSHLERMKQGICLP
                     MAGVIYTDILTDLERISDHCMNIAQDFNEINLVKRNRKVNRPLPRV"
     gene            5850..>6596
                     /locus_tag="P5310_01060"
     CDS             5850..>6596
                     /locus_tag="P5310_01060"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019726360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HRU31984.1"
                     /translation="MDTIALSNRYIELPAFLPDATVGVVKSLDSLDLYNTGVQGIVVN
                     ILHLMRNPGISVIKGIGGLHKFINWNGLILTDSGGFQVFSMIRENSRYGTVTKDGIIF
                     KPSSREKIDLTPEKCIRAQYFCSSDIIMSLDYCTHPDDSYQVNKESVDNTINWAVKSK
                     AEYKKLLEKHPREDKPLIFGIIQGGKDKELREKCAEALIEIGFDGYGFGGWPLDKEGN
                     LLTDILGYTAELMPDNLTKYAMGIGRPENIV"
     assembly_gap    6597..6606
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6607..6945
                     /locus_tag="P5310_01065"
     CDS             <6607..6945
                     /locus_tag="P5310_01065"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019726360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-guanine transglycosylase"
                     /protein_id="HRU31985.1"
                     /translation="VICSKLGYDLFDCVVPTREARHGRLYLFNGDKVEIDLETSSFYS
                     NIFIKEERYTRDERPISESCNCYTCKNYSRAYLHHLFKLEDCLSFRLATIHNIYFYNE
                     LMQLLKEKNR"
     gene            6942..>7203
                     /locus_tag="P5310_01070"
     CDS             6942..>7203
                     /locus_tag="P5310_01070"
                     /inference="COORDINATES: protein motif:HMM:NF018758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU31986.1"
                     /translation="MSFKELEEIVEDILLSSKYKNINKRTISEIAKIESCKYKGKKSL
                     VKAIKNKLHQIYGAFLTERDIEKINRLLDVLETDSRTLKETSK"
BASE COUNT         2164 a         1633 c         1150 g         2246 t
ORIGIN      
        1 aatagaaatg acgatattac tgtagccagc agtcctccta taaacccctc ccaggatttt
       61 aatgggctta atatagatgc aagtttatgt ctcccaaatt ttctaccgac aaaatatgcg
      121 gtggaatcaa aagcccaggt tccaaccata accatggtac accagtatcc ggaaggatcg
      181 aatttcctca taattaaaaa catagagagg ggaacagcca gatatatgag accaaaaata
      241 gtagtcgata ttatggacag gctatcttta atattctgca taattataaa gtaaagcata
      301 agatagaccc aaagcacaat aagtgtctca agaacactgg tagcatattc ccaggaccag
      361 aggagtataa agattccacc gatcagccct tcccaaaatc ttacctttat acccatagat
      421 tctgttaatc tactgtattc aaaaaaaccg ataataaaaa tagccagaag aaaaacttga
      481 agggaaatac tgccaagtct tataagagct agaattaccg ggatacctat aagagcgctt
      541 ataactctct gaaacataat ctcctaaatt cccccaaaac gtctttccct gtgcgtataa
      601 gcctctatag cagaatcaaa ctctttctca tcaaaatctg gccagaagag aggggtaaaa
      661 taaaattcac tataggcaga ctgccaaagg agaaagttac ttattcttaa ctctccacta
      721 gtccttatta cgaggtctgg atcgggaaaa tctctggtat ctaactcctg agagattaat
      781 ctatcattga tatcttcaat atttatctct tttgaagcta tcttgatagc tattctcctt
      841 accgctctta ctatatcatc cctgccactg tagttgatag caaaagcgag agataactta
      901 tcattaaatt tggttgcctc ttcggttaac tccatctctc ttatcacttc ttcagagagt
      961 ctgctcctgt cgccaaaaaa aacaaccttt atcccctctc tgataaattc aggtctttct
     1021 tcctgcaaac ctaacctcaa tatctccatc agaccattga cttcttcttt aggacgtcgc
     1081 caattctctg tagaaaaggc aaaaagagta agatatctta taccaagatt tgccgcatat
     1141 ttcactatac gctttacatt acgctttcct tcctgatgcc cagagattct agataaccct
     1201 ctagcctgag cccatctgcc attcccatcc atgattattg ccacatggac cggtagattc
     1261 tctgacccca ataatctata cctccattat ttccttttct ttttccaata ataccttgtc
     1321 tatctcttct gtatacttat cagtaatctt ctgaagctga tcctttagcc ttttctccaa
     1381 atcctctgaa attttactat tcttcttttc cttttctaac tcttctatcc ctgtccttct
     1441 tatatttctt ataattacct tagcttcctc ccccttcttt tttaccagtt ttaccagctc
     1501 ttttctcctt tcctcagtgg gagatgggat atttagcctt atagaagttc cgtcatttac
     1561 aggtgttaag ctaagattag atttaagaat agccttctct atactgtgca tagactgttt
     1621 atcccagggt tgaataagga tagttctgga atcaggtatg gtgatcttag ctaattggaa
     1681 gaggggtgta gatgtcccat agtaatccac aatcagttct tctacaagag ctggattggc
     1741 tctaccagtt ctgataccac gcaactcctt ctgaaagact gctatggact gcttcatctt
     1801 ctcctctata ccacgagtaa aagccttttc gtccatactt atcactcctt taccagggtt
     1861 ccgatttcct ctccacaaac aagccttttc aagtttccct gctcagagat attaaatact
     1921 attataggta tcttattttc catacatagt gatactgcag tagcatccat aactttcaat
     1981 tctttactta atatatccat atattttatc tcaacatact tagtagcatt tggattttta
     2041 accggatccg agtcatatat accatcaact cttgttccct tgatcatcac ctctgcatta
     2101 atttcggctg ctctcaaagc ggcagctgta tcggtactga aaaaaggatt cccggtacca
     2161 cctccaaaga taaccaccct acccttctca agatgtctta tagctcttct ccgtatatat
     2221 ggttctgcta cctgtctcat ctctatcgag gtctgaaccc tggtcggaat gcccatagat
     2281 tccaaccggg actgcagggc aagagcattt ataatcgttg ataacatccc catataatcg
     2341 gcggttgccc tgtctataga taaggaactg gaggtaatgc ccctgataac attacctccc
     2401 ccaactacta tagcaatctc aactcccaat ctatatatag actggacctc ttcagcgata
     2461 tttacaagag actgatatcc tataggatca gaatcttcct taaaagcttc tccacttaat
     2521 ttaagtaaaa tcctcttata cctaataata tctctcatca gtcttttcct aattcaaatc
     2581 ttacaaatct ccttataaga atattctctc ctaatctggc tatggcatca ttaattatat
     2641 ccttaatctt cttttccggg tcccttatcc agggctgttc taaaagacat acctcctgat
     2701 aaaacttctc cagtttcccc tggacaatcc tctctataac ctgtggaggt ttcccttcat
     2761 tctgagcctg agtagcatag atatctttct cactttccaa aacatcttca ggtatgtcag
     2821 ctattgatat atatctagga ttgctagctg ctatatgcat agcaatctct ttagccagag
     2881 tcttaaactc atctgtcctg gcaacaaaat ctgtctcaca gttaacctca accattacgc
     2941 ccagtttatt cccaggatga atatagcttt ctattcttcc atctttggct agtcttgcgc
     3001 ccttcttctc tgctaccgca ataccctctt ttcttaagat ctccacagcc ttctcaacat
     3061 caccgttggc agagataagt gcctttttac aatccattac cccagcccct gtcttttctc
     3121 tgagttccct tattaaatcc atattaatac tcattctgtc agttcctcct catactcact
     3181 tgccactttt tcttcaatgg catcagctgg taactcctct atgtcaggca ttacaacctc
     3241 ttcttcaacc tcttccttcc tcattcccgt accttcaaga actgcgtctg ccattttagc
     3301 acagtatagt cttatagccc ttatagcgtc atcattaccc gggatcggat aatctattat
     3361 ctcagggtct ccattggtat ccaccacacc aataaccggg ataccaagtc ttttagcttc
     3421 ccttaccgca ttctcttctt tctttgtatc tatcacaaaa agtaaatccg gaggtctatc
     3481 cagattttct atcccgccaa catacttttc aagtctttta agctctatct ccagatgttt
     3541 acgttccctc ttagtataag agtcccacaa gccactatct ctagctgaac gcagttcctt
     3601 taacttttct ataccccttc tcactgtttg gaagtttgta agaagccctc caagccatct
     3661 ctgatttaca taaggcatac cgcatctctc tgcctctgtc tttacaacct catatgcctg
     3721 tttctttgtc cctacaaaga ggacttttcc cccattaccc gaaatgtctc ttgcaacttc
     3781 atatgccttc tccatacaga ccactgtttt ttgaaggtct ataatgtgta tcccattacg
     3841 ttcagcaaag atgaacggtt tcatcttagg attccaacgt ttagtctgat gaccaaagtg
     3901 gactcccgcc tctaaaagct gtttaatagt tactatagcc atacatttgc ctcctctaag
     3961 ttagacttct acctcactac caccctaaat caaagtggac ttagagatgt gaggtatgtt
     4021 aaattcttgt gaaagtataa catatagtta aacccttggc aagggtctgt taacctttct
     4081 atttctcttc accagattaa tctcattaaa atcctgagct atattcatac aatggtcact
     4141 tattctttca agatcagtta atatatcagt atatataact cctgccattg gaagacagat
     4201 accctgtttc atcctttcca gatggctatt cttagactct ttaaccttct ggtctatctc
     4261 ttcttctctt tcagatataa tctccagatt tgaaatatca ctttccctta tcatctcaaa
     4321 tgaatcttcc atattttcta agattaactc tttcaattct ttaaggtcac tctttgcata
     4381 ttccgtaaac tcgactctat ccgagatctt aagttcaatc ttttccataa tatttgttat
     4441 atggtctgct atcctctcca catcgtctac agttctcaat atcgcattta actctcttgc
     4501 ttcatcttca gataatgatg tggcagaaat cctggtaaga tacttaatta aagaggcttt
     4561 aatctcatct gttatatcct ccatagtcag gatatcttta taataattga gagaattctt
     4621 ctcaagatca tcaaaagcca tcatatacat ttctttaaca agatcagtca ttcttaccag
     4681 ctcttttata gcgctttcta atgcggtaga aggggtgttt aacaaaattg gattcagtat
     4741 aagagtagac ctctgaaaaa ctctctcttc tccaggtatt atctttctta taagagaagc
     4801 atactgtctg gtaaaccata tccagataaa ggtccagatc acgttaaaaa gagtatgagc
     4861 atttgctact tgtctagcca catcatcggc tgaaagggaa accagtctaa taaaaggatt
     4921 taaaaatgga aatattagaa ttacacccag aatattaaaa aggagatgcg ctagagctgt
     4981 cctcttggct gaaaggcttg taccaatact ggcaaggaga gcggtcacac atgtacctat
     5041 atttgcccca agaataattg gaatcgccga attcagtggt atcgctccct tagatgccag
     5101 ggcaaccact gtgcttgttg taacactgct gctctgtatg atagcagtca ccgcagctcc
     5161 ggttataatt cctaggatag gatttttacc catattaatc atggcatttg agaatggagc
     5221 ccaatttctg agggttataa gagaggaatc cataagattt aagcccagga aaagcatgcc
     5281 aaaaccaaac acagcttcac ctatatattt tatatatttt cttttcccgg caaatacaag
     5341 taaaaatcca atcagtaaaa agtatggggc aatgactgta agcttaaagg cgacaagctg
     5401 tgcagtaata gtagtaccta tatttgcgcc aaagatgata ccaagagagc cctccagggt
     5461 gagaagcccg gcatttacaa agcccacaac aagcactgtt actgcactgc tactctggac
     5521 tatcgatgtc accacaacac caattaataa tcctctccat ggggaagcgc ttatcttctc
     5581 aaatatagac cttaatcgag agccagctgc cttctctagc ccagaactca tctggaatat
     5641 cccatagagg aacagactta acccacctaa tagaagcccc aaatcttcag gagtaagagg
     5701 aaattcttcc ataccatcag ctccttagtt tatatttttg gtatataaat accagaaatc
     5761 ttagtttcaa ttccaacatc tagtaatatc tatcagagat tatatcatat tgacacagtc
     5821 aataaataac tctaaaatct ctattaagaa tggataccat agccttatct aatcgttata
     5881 tagaattacc tgcatttttg ccagatgcta ctgtcggtgt tgtcaaatca ttagacagtc
     5941 tggacctgta caacacaggg gtacaaggta ttgttgtaaa tatactgcac cttatgagaa
     6001 atccaggaat aagtgttata aagggaatcg gtggattaca taagtttata aactggaatg
     6061 gacttatcct taccgattct ggagggttcc aggtcttttc catgataaga gagaattcca
     6121 gatatggaac cgtaactaaa gatggtatta tctttaagcc ctcatccaga gaaaagatag
     6181 accttactcc agaaaaatgt attcgggctc aatatttttg tagttcagac ataattatga
     6241 gtttagacta ctgtactcat ccagatgatt cgtatcaggt taataaagag tccgtggata
     6301 ataccataaa ctgggctgtt aaaagtaagg ctgaatataa aaaacttcta gaaaaacatc
     6361 ccagagaaga taaaccttta atttttggaa taatacaggg aggtaaggat aaagaactaa
     6421 gggaaaaatg tgcagaggcc cttatagaga taggattcga cgggtatgga tttggggggt
     6481 ggcctctcga taaagaggga aatctgctta cagatatcct gggttatacc gccgagctta
     6541 tgcctgacaa ccttaccaaa tatgctatgg gtataggcag accggaaaat atagttnnnn
     6601 nnnnnngtta tatgctcaaa acttggctat gacctcttcg attgcgttgt cccaaccaga
     6661 gaagcaaggc acggaaggct ctacctgttt aatggagata aggtagaaat agaccttgag
     6721 acgagtagtt tttacagtaa tatctttatc aaagaggaga gatataccag ggatgagaga
     6781 ccgatttcag agtcctgtaa ctgttatacc tgcaaaaatt actcaagggc atatcttcat
     6841 catctcttca aattggagga ttgcttgagt tttagattgg caacaataca taacatctat
     6901 ttttataatg aacttatgca attactcaaa gagaaaaaca gatgagtttt aaagagctag
     6961 aggaaatagt tgaagatata cttctctcca gtaaatataa gaacataaat aaaagaacaa
     7021 tctcagagat agcaaagata gagtcttgca aatataaagg gaaaaaatct ctggtcaagg
     7081 cgattaagaa taaacttcat cagatatacg gagcctttct aacagagaga gacatagaaa
     7141 agataaatag acttttagat gtactggaga ctgatagtag aactctgaaa gaaacatcaa
     7201 aga
//
LOCUS       DAOTKU010000022         7167 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_10077_length_7167_cov_3.000422, whole genome shotgun sequence.
ACCESSION   DAOTKU010000022 DAOTKU010000000
VERSION     DAOTKU010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7167
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10077_length_7167_cov_3.000422"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..869)
                     /locus_tag="P5310_01075"
     CDS             complement(<1..869)
                     /locus_tag="P5310_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HRU31987.1"
                     /translation="MSKIAVIGAGSVAFSMSLIQDFALTKGLSGCTVTFMDINQDRLS
                     MVYGLASRYIKELDMDLNLEKTTSREEAIREADFVINTVKVDGYDFMEAEREIAEKHG
                     YYRGIDDKVSDYYGGFFAYKQLNFFLDLARDVERISPNAWFMQVSNPVFEGTNIIARE
                     TGLKVAGFCHGAQGYKDIVKILKLNPEEVEAQVVGLNHCNWLTRFLYRGNNAYPLIDE
                     WIEKESENYWKSDEYLHNPWGYHITPAAVEMYKLYGVFPVGDSVRSVSPWWFNTDLET
                     KEKYFSSGGPDSEV"
     gene            1111..1533
                     /locus_tag="P5310_01080"
     CDS             1111..1533
                     /locus_tag="P5310_01080"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HRU31988.1"
                     /translation="MENAGEILRKIGEKITEQREAILQAIIDLARPVSADELYEYLLT
                     TNKVGRTTIYRTLELFEKKKLVRKVLFKDGIIRYESSFLKHHHHLVCLSCGKILPLDG
                     CFVAPVEGAILKGSDFLVTDHYLELYGYCAKCRRERER"
     gene            1530..2387
                     /locus_tag="P5310_01085"
     CDS             1530..2387
                     /locus_tag="P5310_01085"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HRU31989.1"
                     /translation="MKKFILTMILVLTLTQSFAQEKPDVVVSFFPLYDFTRNIGGDRI
                     DLTSLIPFSVEPHDWEPGPRDMVKVSSADIFIYNGAGLEPWADDIIKSVRNKKLKVVD
                     ISKSIRVTGEDPHIWLDPILVKSQLKVIKDTLIGYDPKNRAYYDKNYNIYLKKIEELD
                     REIRKTIARCKKKVFVTSHDAFSRFAERYGLTQVPVMGISPESEPSPRELAGIIETIK
                     RYDVKYIFTEPFIPIKIAESISRETGVKILVLDPIEGLTEEEMKSGADYLSKMKKNLE
                     NLKIALEYE"
     gene            2380..3084
                     /locus_tag="P5310_01090"
     CDS             2380..3084
                     /locus_tag="P5310_01090"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HRU31990.1"
                     /translation="MSDKILEVRGLYFSYDGENVLEDITFSLDRGEFLGIIGPNGSGK
                     STLLKLITGILKPDRGEIKLYTDRISYIPQKITSLSYNFPATVEEIVSLGILSKGMLK
                     RISRKDYNLIEQALDKVGLLELKNKRLGELSGGQQQRVHIARAIVSNPELLVLDEPTT
                     GIDIVNQTRFYELLGHLNREMGITVIIVSHDISTIAGEVTTMAYLDKKLYFIGKPKDF
                     FKKILWPVELEDLLNR"
     gene            3081..3902
                     /locus_tag="P5310_01095"
     CDS             3081..3902
                     /locus_tag="P5310_01095"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HRU31991.1"
                     /translation="MMDILQYSFMRYALMSGIFVGILCPIIGVFLVLKNLSLIADGMA
                     HFSFGGIALGIVLGISPTYMILLFSILGGLLINYVIRRTKLYGDTVIAILLSTGLAIG
                     ITIISLARGTNFNINAYLFGSLLAIDEKELMSIILLGIPAIILVIIFYRPLLYITFDE
                     DSAKASGLPVESINILLVILSAVTIAITIKVAGILLTSALIVIPVATGIQISNSFKMT
                     LLSSVVTGIISVILGLIISYYLNIAPGGAIVLVSVLIFLLSISGLFDQKGYYSKT"
     gene            complement(3828..5114)
                     /locus_tag="P5310_01100"
     CDS             complement(3828..5114)
                     /locus_tag="P5310_01100"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRU31992.1"
                     /translation="MQNRNTRDVEEFVARYYKRNFIVNSLDMAFFSFGSAFYSISTIF
                     PVLIKNLGGSNIHIGLLPAIANLGWSIPSIIGAKMVAGSSKKLPFILRWTIGERLPYL
                     IIAFLLIFLVPSNPSLAMYLALFLLFIATFTGGILSPAWLDYIAKIIPPSRRGTIFSI
                     GFGMGGVLGILASFLARRFLEIYPFPYNFSYCFICAGIALGISYLFLALAKEPEVTVE
                     SDDLDLVQYMKSLPFILRSDKNFTLYIIARALSAFGSMGVGFYTVYSLNTFNLHDGYA
                     GIFSAFLLGSQTIATFLLGPIGDLKGHKLILSIGILSIILGYLSLVIDTTLWMAYLAF
                     ILMGINYSAFGTSGMAIIMDLAPEEKRPVYAGLSNTLMGPFSFAAPIIGGVLANRFGY
                     KPMFIIGLSLSVLSFTIITFLVKEPRNTQQKYQDTD"
     gene            complement(5087..6478)
                     /locus_tag="P5310_01105"
     CDS             complement(5087..6478)
                     /locus_tag="P5310_01105"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRU31993.1"
                     /translation="MKILLVNPPAASYYHRLGLKYPPLGVGYIAGVLVKGGYDVKVID
                     LNVEKVSINNIPYQDFDIVGVSTDTTRYPAALKIAQAAKAKGCKVVVGGPHTAFLDRE
                     ALETGFIDYVVRGEGEYIMLDLMDAIMGKKSLEDVKGISYIKDGTIIRNPNAELIKDL
                     DRIPFPARNLLPMDKYTAKLEGTFATSIVTSRGCPFNCSFCSSSAFSGVQWRSRSVEN
                     IIEELEILYKKYGFRAIAFFDDNFTLNPNRVINLCEEILRLGWKFKWWAFSRLDGILN
                     NPKMVELMAKAGNYMVFIGFESANQEVLDSYNKKLVVEKAKEAINILRKNNIKIMGSF
                     ILGAMEETKAMVNRTIKYAKELDPDIAQFSVLTPYPGTALFEEVKSKIMDRNWEMYEG
                     GHNVFKLKYLSPVEVRWLIAKAYMSFYGRPKRLITQGIPSLWNMLIGYRVNERVPLKF
                     VEGDWCRIETQEM"
     gene            complement(6482..>7167)
                     /gene="rfbD"
                     /locus_tag="P5310_01110"
     CDS             complement(6482..>7167)
                     /gene="rfbD"
                     /locus_tag="P5310_01110"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:TIGR01214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HRU31994.1"
                     /translation="DVVINCSAFTDVDLCEREIDRAFNVNAIGAKNLALASRKYDTHL
                     IHISTDYVFDGRKDSPYIEFDRPNPINIYGKSKLEGENFVKTFSQKFTIVRTSWLYGD
                     GNNFVKKIVERARQEKVIKVVDDQFGSPTYTYDLAEALLAIAKSEVYGLYHFANTGVV
                     SRFQEANFIIERLKLDAEVIPVKTEEYVTPAERPRYTPLKNYLLELEGVFYGRDWKSA
                     IEEFLFKER"
BASE COUNT         2085 a         1486 c         1370 g         2226 t
ORIGIN      
        1 acctctgaat ctggtccgcc ggaggaaaaa tatttttcct ttgtctctag gtcagtgttg
       61 aaccaccacg gggatacgct tctcacagaa tctcctacgg ggaatacacc atatagttta
      121 tacatctcaa ctgcagctgg agtaatatga tatccccagg gattatggag atactcatcg
      181 ctcttccaat aattttcaga ctccttctct atccattcgt ctatcagtgg ataggcatta
      241 tttcccctgt ataaaaatct ggttaaccag ttacaatgat ttaagcctac aacctgtgcc
      301 tcaacctctt ccggattgag tttcaggatt tttactatat ctttataacc ctgagcccca
      361 tgacagaacc ctgccacctt taaccctgtc tccctggcta tgatgtttgt tccttcaaat
      421 actggattgg atacctgcat aaaccaggcg ttcggactta ttctctccac atctcttgcc
      481 aggtctagga agaagtttaa ctgtttatag gcgaagaatc ctccatagta atcactcacc
      541 ttatcgtcta tccccctgta gtatccatgt ttctctgcga tttctctctc tgcttccata
      601 aagtcataac catctacctt gactgtattt ataacaaaat ctgcttctct gatagcctct
      661 tctcgactgg tggttttttc tagattcagg tccatatcta attcttttat atacctgctc
      721 gccagaccat ataccatact gagtctatcc tggtttatat ccataaatgt aactgtacag
      781 ccagagagtc cttttgtgag ggcaaaatcc tggatcagac tcattgaaaa tgctacactt
      841 ccagcaccaa tcaccgctat ttttgacatc tctattccct ccttttgaaa gtttcttcag
      901 atttatcccc attatagcat aagctggttc cttatcaaag tttcattttt catctataat
      961 aaagataaaa tattggagag gaggtttgaa tatctctctg acttacacag gttaatggtt
     1021 gaaaaggcta ataagtctat tccggctgtt gacttttctt atttttagtt tataatttta
     1081 aatgaaactt agttccattt agaggtaagg atggaaaatg ctggtgaaat actcagaaaa
     1141 ataggagaaa agattactga acagcgcgag gctattctgc aggctattat agatttagcc
     1201 cgtcctgtca gtgctgatga actttatgag tatttattaa ccactaacaa ggtgggacga
     1261 actaccattt accggacact cgaacttttc gagaagaaaa aactggtaag aaaggtttta
     1321 tttaaagatg gtattatcag gtacgagtct agctttctca agcatcatca tcaccttgta
     1381 tgcctgagtt gtggaaagat tttaccccta gatggatgct tcgttgcccc tgtagaaggg
     1441 gctatactta aaggttcgga ttttcttgtt acagaccact atctagagtt atacggatac
     1501 tgcgcaaaat gcaggagaga gagggaaaga tgaagaaatt tatcctgact atgatacttg
     1561 ttttgaccct tactcaatct tttgctcagg agaaacctga tgtggttgtg agcttcttcc
     1621 ccctgtatga ttttaccagg aatataggtg gagataggat agatctaacc tcccttatac
     1681 cattcagtgt tgaacctcat gattgggagc caggtcccag agatatggta aaggtatctt
     1741 cggcagacat ctttatatac aacggtgcag gtttagaacc ctgggctgat gatataataa
     1801 agagtgttag gaacaagaag cttaaagttg tagatatttc aaaatctata agagtaactg
     1861 gagaagaccc ccacatatgg cttgacccga tcctggtcaa gtctcagcta aaggttataa
     1921 aggatacttt gatagggtat gatccgaaga atagagcgta ttacgataaa aactataata
     1981 tctatcttaa gaagattgaa gagctggata gagagatcag aaaaactatt gccaggtgta
     2041 agaaaaaggt ctttgtaaca tctcatgacg cattctccag atttgccgaa aggtatggac
     2101 ttacccaggt accggttatg ggcatctcgc ctgagtctga accgagtccc agggaacttg
     2161 ccgggattat tgaaacgata aagagatatg atgttaagta tatattcaca gaaccattta
     2221 tacccatcaa gatagctgaa tcaatctcca gagagactgg ggtaaagata ttggttctgg
     2281 atccaatcga aggattaact gaggaagaga tgaaatctgg agcagattac ctctctaaga
     2341 tgaagaaaaa tctggaaaat cttaagattg cccttgaata tgagtgataa aattttagag
     2401 gttagaggat tatattttag ttatgatggt gaaaatgtcc tggaagatat cactttttca
     2461 ctggacaggg gagagtttct tgggattata ggacctaatg gttccggtaa aagcaccctt
     2521 ctgaaactta taaccggcat cttaaagcca gataggggtg agattaagct atatactgat
     2581 aggataagtt acatacctca gaagattaca tctctatcgt ataatttccc agctacagtg
     2641 gaggagatag tctctcttgg aattttatct aaggggatgc tgaagagaat atcaaggaag
     2701 gactataacc ttatagaaca ggctcttgat aaggttgggc tcttggagct taaaaataaa
     2761 aggctcggtg agctctcagg tgggcagcag cagagggtgc atatagccag ggctattgtg
     2821 agtaatccag aactccttgt tttagacgaa ccaactacag ggatagatat agtcaaccag
     2881 accaggttct atgaactcct tgggcatctg aatagggaga tgggaataac agtgataatt
     2941 gtctctcatg atataagtac catagcaggt gaggttacta ctatggcata tctggataaa
     3001 aagctttatt ttatagggaa accaaaagac ttcttcaaga agattctctg gccggtagag
     3061 ctggaggatt tactgaacag atgatggata ttcttcagta ttcatttatg aggtacgccc
     3121 tcatgtcagg tatatttgtt ggtatcctct gtcctatcat aggtgtattt ctggtcttga
     3181 agaatctctc cctcatagcg gatgggatgg ctcatttctc ctttggcggt atagccttag
     3241 gaatagtcct ggggataagt cctacctata tgatcctact cttttctatc ctgggaggtc
     3301 ttttgataaa ctatgtaata agaagaacga aactttatgg tgataccgtt atagcgatac
     3361 tcctctccac cggacttgcc atagggataa caattataag tctggcaagg gggacaaact
     3421 tcaatataaa tgcctatctc tttgggagtc ttctggctat agatgagaaa gagcttatga
     3481 gtataatcct tcttggtata ccagctatta tccttgttat aattttttac cgaccccttt
     3541 tatacataac ctttgatgaa gattctgcca aggcaagtgg actcccggta gaatctatca
     3601 atatactcct tgtgatcttg agcgcagtta ccatagctat cactataaag gtggcaggaa
     3661 tccttttaac gtctgctctg atagtgatac ctgtagctac aggtattcag atctctaata
     3721 gttttaagat gacactccta tcctcggtag ttactggaat catctctgta atccttggac
     3781 tcattatctc atattatctg aatatagccc ctgggggagc gatagttcta gtcagtgtcc
     3841 tgatattttt gttgagtatt tctgggctct ttgaccagaa aggttattat agtaaaactt
     3901 aagacactta aagatagacc tattataaac atcggtttat atccgaatct attggctaag
     3961 acccctccta ttattggggc tgcaaagctg aaaggaccca tcagtgtgtt tgaaagtcca
     4021 gcatatacag gtcttttttc ttctggagcg aggtccataa ttatagccat tccagatgtt
     4081 ccaaatgcgc tgtagtttat acccataagt ataaatgcca ggtacgccat ccatagagta
     4141 gtatcaatta caagacttaa atatccaaga attatagaga ggataccaat ggataagata
     4201 agtttgtgcc cctttaaatc tccaatcggt ccaagaagaa atgtagcgat agtctgtgaa
     4261 cctaaaagga atgcactgaa tatcccggca tatccatcgt gaaggttaaa agtattgagg
     4321 ctatagacag tataaaatcc tactcccata ctgccaaaag cagacaaagc cctggctata
     4381 atatagagtg taaagttttt gtcggatctt aatataaagg gaagactttt catatattga
     4441 actaaatcta aatcatctga ttccacggtg acttctggtt cttttgccaa agccaggaag
     4501 agataagata tacccaaagc tatacctgca catataaaac agtaggagaa gttataggga
     4561 aacgggtaaa tctcaagaaa tctcctcgct aaaaatgacg ccagtatccc cagcactccc
     4621 cccataccaa agcctataga aaagatagtc cctctccttg aaggtgggat aatctttgct
     4681 atatagtcca gccaggcagg ggagagtatc cctccggtga aagttgcgat gaataacagg
     4741 aatagtgcta aatacatagc taagctcgga ttcgaaggaa ctaaaaatat cagcagaaag
     4801 gcgataatca gatatgggag tctttccccg atggtccatc ttaagataaa tggtagtttc
     4861 tttgacgatc cggctaccat tttagcacca attatactcg ggatactcca acccaggttg
     4921 gctattgcag gtagaagtcc tatatgtatg ttgctgccac ccagattttt aataagtaca
     4981 ggaaatatgg tggatatgga ataaaaggct gatccaaaac tgaagaaagc catatctaga
     5041 gagttgacga taaagttcct cttatagtat ctagcaacaa actcttctac atctcttgtg
     5101 tttctattct gcaccaatct ccctctacga atttgagtgg aaccctttca tttactctgt
     5161 aaccgatcag catgttccat aagcttggta taccctgtgt gataagtcgc tttggtcttc
     5221 cgtagaagga catataagct tttgctatga gccatctcac ctctaccgga gagagatact
     5281 taagtttgaa aacattgtga ccaccctcat acatctccca gtttctatcc ataatctttg
     5341 actttacctc ttcaaacaga gcggtcccgg gatagggggt aagtactgaa aattgggcta
     5401 tatctggatc aagttccttt gcatatttta tcgttctgtt taccattgcc ttcgtctctt
     5461 ccattgcacc caggataaaa ctccccataa tctttatatt gtttttgcgc aagatgttta
     5521 tagcttcttt agctttttct accacaagtt tcttattgta gctgtcgagc acctcctgat
     5581 ttgcactttc aaagccgatg aaaaccatat agttacctgc ttttgccata agttctacca
     5641 tcttggggtt attcagtatt ccatccaatc tggaaaatgc ccaccatttg aacttccagc
     5701 ctaatctgag tatctcctcg cataaattaa tgaccctgtt agggttcaga gtgaagttat
     5761 cgtcaaagaa tgctattgcc ctgaacccgt actttttata aagtatctcc agttcttcta
     5821 tgatattttc tacacttcta cttctccact gtacacctga gaaagcggaa gaagagcaaa
     5881 aactacagtt aaaaggacac ccccttgatg taactattga tgtagcgaat gtcccttcca
     5941 gttttgccgt gtatttatcc atagggagga gatttctagc tggaaatggt attctatcca
     6001 ggtctttgat taactctgca tttggatttc ttatgattgt tccatctttt atatacgata
     6061 ttccctttac atcttccagg ctctttttcc ccataatcgc atccataagg tccagcatta
     6121 tatattcccc ttctcccctc accacataat ctataaatcc tgtctctaaa gcctctctat
     6181 ccaggaaggc tgtatggggt cctcctacaa ctactttaca tcctttagct ttggctgcct
     6241 gagctatctt gagagctgca ggatatctcg ttgtgtcggt ggatacacca acaatatcaa
     6301 aatcctgata tggtatgtta tttatactta ccttctcgac gttcagatct attaccttta
     6361 catcatatcc accttttacc aggactccgg ctatataacc aacaccaagt ggtggatatt
     6421 taagcccaag cctgtgataa tagctggcag caggtggatt tactagaagt attttcatcg
     6481 cttatctctc cttaaataag aactcttcaa tagcagattt ccagtctctt ccataaaata
     6541 ccccctctaa ttccagcaga taatttttta atggtgtgta tctgggtctc tccgccggag
     6601 taacatactc ttcagttttt accggtataa cttctgcatc taacttcaat ctttcaatta
     6661 taaagtttgc ctcctgaaac ctggatacca cacctgtatt cgcaaagtga tataaaccat
     6721 aaacctcact cttagctata gctaaaagtg cttcagccag gtcatatgtg taagttggag
     6781 agccaaactg gtcgtctaca accttaatga ccttttcctg tcttgccctt tccactatct
     6841 tttttacaaa attattacca tctccataca accagcttgt ccttacaata gtaaatttct
     6901 gtgagaaggt tttaacgaag ttttctcctt ctaacttgct ctttccgtag atatttatag
     6961 ggtttggtct atcgaactca atataaggtg agtcttttct accatcgaag acataatcgg
     7021 tacttatatg tatgaggtgc gtatcatact ttcgggatgc caacgccaga ttttttgctc
     7081 caatagcatt aacattaaag gctctatcaa tttctctctc acaaagatcc acatctgtaa
     7141 aggcagaaca atttatcacc acatcgg
//
LOCUS       DAOTKU010000023         7101 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_10182_length_7101_cov_3.174567, whole genome shotgun sequence.
ACCESSION   DAOTKU010000023 DAOTKU010000000
VERSION     DAOTKU010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7101
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10182_length_7101_cov_3.174567"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1045)
                     /locus_tag="P5310_01115"
     CDS             complement(<1..1045)
                     /locus_tag="P5310_01115"
                     /inference="COORDINATES: protein motif:HMM:NF017321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HRU31995.1"
                     /translation="MGDLFNFGTEKKVPLAARVRPETLSEFLGQRHLVGDNGFIKRII
                     EKGEPPPSIILWGPPGCGKTTLALIIAKSSRCNFIKVNAVLSGVAELKKAFAKAEEDR
                     KLYSRKTILFIDEIHRFNKAQQDVLLPYVEDGTIILLGATTENPYFTLNGTLISRCRV
                     LKLEPLTDEDISRIIDRAISEEEGLGSFNIRLTEPARALIMRYANRDARQALNLLEES
                     ALLVRGENRDTIEPSDIERLFTERLFKYDEHGEEHYNLISAFIKSLRGSDPDASVYWL
                     VRMIDSGEDPRFIARRMIIQSAEDVGNADPMALVIAVNASQALEYVGLPEAIIPLVQA
                     AIYIASAPKSNSAY"
     gene            complement(1049..1294)
                     /locus_tag="P5310_01120"
     CDS             complement(1049..1294)
                     /locus_tag="P5310_01120"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRU31996.1"
                     /translation="MFAIWIIFLVVILIFWLVGRNKRKNQKKCPFCGRMIPKDADNCP
                     YCGKGLSEVCQYCKMPVEPNWGVCPYCGKKIDRHRLV"
     gene            complement(1306..1704)
                     /locus_tag="P5310_01125"
     CDS             complement(1306..1704)
                     /locus_tag="P5310_01125"
                     /inference="COORDINATES: protein motif:HMM:NF016160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HRU31997.1"
                     /translation="MLSEPVSNGKVVKVEGDTVEIAVPRSERCKSCGICPISADGSVL
                     LKVNVDRDVKVGDEVRIRTKDKYVILSAFVIYIVPVIALITGYFLGGLLFSSELLKIV
                     SGFLFMGISFLIDRFLDKKIRFPQEIELIV"
     gene            complement(1694..1987)
                     /locus_tag="P5310_01130"
     CDS             complement(1694..1987)
                     /locus_tag="P5310_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU31998.1"
                     /translation="MGLFSRLFQKSPQQDRNFYYTVKCNRCGEEIRVRMDRMNEPSPE
                     YDEKGRVTHYIYRKDVLGQKCFNLIQVEFVFNSNFEMISSQVTGGKLIEPDVK"
     gene            2347..>2866
                     /locus_tag="P5310_01135"
     CDS             2347..>2866
                     /locus_tag="P5310_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA/Hda family protein"
                     /protein_id="HRU31999.1"
                     /translation="MEQISEMLTIWNSLLEELEPVLSPASFETWLKTARLIEIRDNSA
                     LIGVPNTFAKEWLETRLFPTLKSTLSALAGKSIDVRIIVDDTLIEREPVIEVKNEQRE
                     QTTPQLNNRYIFDTFVVGSGNRMAHAAALAVAENPGKAYNPLFIYGGVGLGKTHLMQA
                     IGHYIIAKKRGLK"
     assembly_gap    2867..2966
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2967..3845
                     /gene="dnaA"
                     /locus_tag="P5310_01140"
     CDS             <2967..3845
                     /gene="dnaA"
                     /locus_tag="P5310_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014901041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HRU32000.1"
                     /translation="HLMQAIGHYIIAKKRGLKVVYISSESFTNELINSIRDHTTVEFR
                     GKYRSIDLLLIDDIHFLAGKETTQEEFFHTFNALYEAGKQIVISSDRPPKEIPTLEER
                     LISRFEWGLTVDIQPPDLETRIAILRKKAELDLISVPDEVINFIAERVVSNIRELEGA
                     LIKVIASASLTKRPITLELAQECLAPLFLNGQEKKHPATIPEIQKVVAEYYGISIDEI
                     RGQRRTRDSVFPRQVAMYISRELTNASLPAIGREFGGRDHSTVIYAYEKIKETMRQDS
                     EVAKTINQLINRFIRV"
     gene            4060..5142
                     /gene="dnaN"
                     /locus_tag="P5310_01145"
     CDS             4060..5142
                     /gene="dnaN"
                     /locus_tag="P5310_01145"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HRU32001.1"
                     /translation="MKFLCNRKELEKMLTIISKVIPPRSTKEGLNSLLIKTEPPDRLY
                     FYATNIEMSIEAVLNCNTDESQSFLVSAKLFKDIIDSLSTDSVAGLIEDKTFDIIAGE
                     TVYHIPTQNIIDFPVPEEFIPEREIHINSEMLRSAVKKVLPFVDKTSIRPVLSGVFID
                     PREDEVRLVATDANRLAVKKISMENIGNSVILPALSLETFTSADLDEEIGMGFGNSKV
                     RLKSSNISLTTSIIDGTFPQYERVIPEEFITSIRVERDVLREAIKRVLPISREEDYAV
                     KFAIKSRKLILNARSPIGVVFDELDIETEGEDIEIIFSANYITDFLSVGDGILNIKIS
                     GEKDPAIFTFDNDPDFIYVTMPMESE"
     gene            5139..5354
                     /locus_tag="P5310_01150"
     CDS             5139..5354
                     /locus_tag="P5310_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009673395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HRU32002.1"
                     /translation="MKEVYILTETITLSQFLKWAGIAQTGGEAKLLIQQGGVTVNNKV
                     ETQRGRKLRENDIISVQGQGEFIVKRK"
     gene            5357..6439
                     /gene="recF"
                     /locus_tag="P5310_01155"
     CDS             5357..6439
                     /gene="recF"
                     /locus_tag="P5310_01155"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HRU32003.1"
                     /translation="MILDSIELLSFRNFPHLMVNFNPNFNIFIGPNGAGKTNLLEAIY
                     LLSNGELKRGKREIEAIKWNDPFTWIRGEVEYEDGRLMKLVTQIRIEGGKDFSINGKI
                     VSLRRYLGSFPTVSIFDDDQDIITGGPDRRRKFLDDFVISIDPEYYSILNSYKGILTR
                     RNKVLRTKNTDKKLLEALTELLLDYGEKIIASRYNAVSVYNRYLDREGKKFSLEIRVE
                     IPSFKEDIQKSIQFYRDRFNLFRRKEETIGITLIGPHREDIIIRINGKDSRSFASNGE
                     IQIILFLLKVAQFEIIRETKSYKPVFLIDEFLGRLDDKNSRLIMDLIEEKSPQIFTTM
                     LFKRVVGKSGSIFTIDNGEIIDEEAF"
     gene            6423..6842
                     /locus_tag="P5310_01160"
     CDS             6423..6842
                     /locus_tag="P5310_01160"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HRU32004.1"
                     /translation="MKKLSNVLLELPLRRFFILPIAWNKIVGKEVARFAHVTYYKDGV
                     LYIGVPAAVWANELSTHSPKIIEEMSKETGIPITEIRFRVYPFYIREEKELNLDELTE
                     EDKKYIDSIVANVKYERIRESLFKIIGYHILRTRHGI"
     gene            complement(6854..>7101)
                     /locus_tag="P5310_01165"
     CDS             complement(6854..>7101)
                     /locus_tag="P5310_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRU32005.1"
                     /translation="TASLFSFYWTWDDFLGPLLYLNQATLYTVSLALKNFADPTTITN
                     WGAVFAMSVLSLMPVLLIFIFFQKYLTEGIATTGMKG"
BASE COUNT         2290 a         1333 c         1410 g         1968 t
ORIGIN      
        1 tataagcaga gttgctcttt ggagcactgg ctatatatat ggctgcctga accagaggta
       61 ttatagcttc aggcaatcca acatactcca gagcctgact ggcatttact gcaattacca
      121 gagccatggg gtctgcatta ccaacatctt ctgcagattg tattatcatt cttctggcta
      181 taaatctcgg gtcttcccca gaatctatca tcctcacgag ccagtataca ctggcatcag
      241 ggtctgatcc tctcagactc tttataaacg cagatataag gttatagtgc tcctctccgt
      301 gctcatcata cttgaaaagc ctctcagtaa ataatctctc tatatcagac ggttcgattg
      361 tgtccctgtt ttcacctcta acaagaagag cagattcttc aagaagattc agagcctgtc
      421 ttgcatctct gtttgcatat ctcattataa gggctctggc aggttcggtt aaccttatat
      481 taaaagaccc cagaccttcc tcttcgctta tagctctatc tataattcta gatatatctt
      541 catctgttaa cggttcaagt tttaataccc tgcatcttga tatcagggta ccgttaagag
      601 tgaagtaggg gttctcagtg gtagctccaa gaagtattat agtcccatct tccacatatg
      661 gtaatagcac atcctgttga gctttgttaa acctatgaat ctcgtctata aaaagaatag
      721 tctttcttga atagagcttc ctatcttcct ctgctttggc aaaggctttt tttaactctg
      781 ctaccccact caaaaccgca ttcaccttta taaaattgca tctggagctt ttagctatta
      841 tcaatgcaag tgtagtcttt ccacacccgg gaggacccca gaggattata gatggaggag
      901 gctctccctt ctctataatc ctctttataa agccgttatc tccaaccaga tgtctctgcc
      961 ctaaaaattc tgacagagtc tctggtctta ccctggcagc taacggaact tttttttcag
     1021 taccaaaatt aaacagatca cccataaatt atactaacct atgtctatcg atcttcttac
     1081 cacagtacgg acataccccc cagttaggct caactggcat cttgcagtac tgacatacct
     1141 ctgaaagccc ctttccgcag taaggacaat tatcagcatc ctttggaatc atcctcccac
     1201 agaatggaca tttcttctga ttcttcctct tattcctccc aacgagccaa aaaataagaa
     1261 taacaactag aaaaattatc catattgcga acaattgctt cctccctaaa ctattaattc
     1321 tatctcttgg ggaaacctaa tcttcttatc taaaaacctg tcaattaaga aagatatacc
     1381 cataaataaa aaaccagaaa caatctttaa taattcagat gaaaaaagaa gacctcccaa
     1441 aaaataacca gtaatcaacg cgataacagg cacgatataa ataacaaagg cagacaaaat
     1501 tacatactta tcttttgtcc ttattcttac ctcatctcca actttcacat ccctatccac
     1561 gttaactttc aataataccg aaccgtctgc agatatagga cagattccac aagacttgca
     1621 tctttcactc cttggaacag caatctccac agtatctcct tcaaccttta ccacttttcc
     1681 atttgaaact ggttcactta acatcaggtt ctattaattt cccccctgta acctgagaag
     1741 atatcatctc aaaattagaa ttaaagacaa actcgacctg gatgagatta aagcacttct
     1801 gtccaagcac atccttacga tagatataat gagttaccct tcccttttca tcgtactcag
     1861 gggaaggttc attcatcctg tccatcctta cccttatctc ttctccacat ctattacact
     1921 tcacagtata ataaaaattc ctgtcctgct gagggctctt ttgaaagagt ctggagaata
     1981 aacccattca gaaatctcct tattaaaccc cgccatgccg tatgcgcttt gttcccgaac
     2041 cccggaaaca acttttgtgc cttcccagga atttcatgaa gctcctctcg aagcctctaa
     2101 tccattcctg gagtgaggca caccacgtgt aagtgttggc tcggaacacg tcttgcgcaa
     2161 ctcgcacatg gcagggttat tttccattta tataatatca taacataaga ataagttcaa
     2221 tataatcccc ctgatctatc tatatccaag aattaatgat acttaagacc gcttttatta
     2281 aaatacccca cctttacacc tctatatctt ttatagtaaa atccacttaa gagtgggagg
     2341 gatagtatgg aacagatatc agaaatgtta acaatatgga atagcttact tgaagagctg
     2401 gaacctgttt taagcccggc cagttttgag acctggctta aaacggcaag attgatagaa
     2461 atcagagata acagcgcact tataggtgtg ccaaatacgt ttgccaagga atggctggag
     2521 accagattat ttcctacttt aaagagcaca ctatctgctc ttgctggaaa gagtattgat
     2581 gtaagaatca ttgtggatga tacccttata gagagagaac ctgttataga ggtaaaaaat
     2641 gagcagagag agcagaccac cccacagcta aataacagat atatatttga tacttttgta
     2701 gtgggaagcg gaaacaggat ggctcatgca gctgcgcttg ctgtagctga aaatccaggt
     2761 aaggcatata atcctctctt tatatatgga ggagttggtc tgggtaaaac acacctgatg
     2821 caggcgatag gacattatat aattgccaag aagagagggc tgaaggnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnncacc tgatgcaggc gataggacat tatataattg
     3001 ccaagaagag agggctgaag gtcgtctata tatcatcaga aagctttaca aatgagctta
     3061 taaactctat aagagaccat acaacagtgg agtttagagg aaaatataga agtatagacc
     3121 ttctcctgat agatgatata cattttttag cagggaagga aaccacccag gaagagttct
     3181 tccacacatt taatgcgctg tatgaggcag gtaaacagat agttatatcc agtgatcgtc
     3241 cacctaaaga aataccaaca cttgaagaga gacttatatc cagattcgaa tggggattga
     3301 cagttgatat acagccacct gacctggaga ccaggatagc tatattgagg aaaaaagcag
     3361 aactggacct gatatctgta cctgatgagg ttataaactt tatagccgaa agggttgtat
     3421 caaatatcag agagcttgaa ggcgcactta taaaggttat agcgtccgcg tccctgacaa
     3481 aaagacccat aacccttgaa ttggctcaag agtgtctggc tcctctcttt ctgaatggtc
     3541 aagagaaaaa gcatcctgct actatcccgg agatacagaa ggtggtagca gaatactacg
     3601 ggataagtat agatgaaata aggggacaga gaagaacaag ggattcagtc tttccgaggc
     3661 aagtagcgat gtatatatcc agagaattaa ccaatgcttc tctgccggct atcggcagag
     3721 agtttggagg gagagaccac tctaccgtta tatatgccta tgaaaagatc aaagagacaa
     3781 tgcgacagga ttcagaggtc gccaaaacta tcaatcaact tattaacagg tttatcaggg
     3841 tataatttgt ggaatctgtg gatagttgac ccctgttatg tggaaattgt tgataacctg
     3901 tggaaaacta acaacttatc cacaggttta tccacaggat gcaatcccaa aaataaaaca
     3961 atatacgaat ttatccacag aactaaccga cctactactg ttactaatct tttaaaagat
     4021 attagaagta tatattagta aatcaacagg aggagacata tgaaattttt atgtaacagg
     4081 aaagaactgg aaaagatgct tacgataatc tccaaggtga taccacctag gagtacaaag
     4141 gaaggtttaa attctctcct tataaaaaca gaaccaccgg atagattata tttttatgcc
     4201 accaatatag agatgagtat agaggcagtt ttaaactgta ataccgatga gtcgcagagt
     4261 tttcttgttt ctgctaaatt attcaaggat attatagata gtctcagtac cgattcagta
     4321 gctggactta tagaagataa aacattcgat atcatcgctg gagagaccgt atatcacata
     4381 cctactcaaa atataataga ttttcctgta ccagaagaat tcatacctga aagggagata
     4441 catataaatt cagagatgct caggtcggct gtaaaaaagg ttttaccatt cgttgataag
     4501 accagtataa gacctgtact atccggagta ttcatagacc ccagggaaga tgaagttcga
     4561 ctggtagcta cagatgcaaa tagactggcg gttaaaaaga tctctatgga gaatataggg
     4621 aacagtgtta tactcccggc actcagtctg gagacattta cctctgcaga tctggatgag
     4681 gagataggta tgggctttgg taattctaaa gtgagactta aaagcagtaa tataagtctc
     4741 accacttcta ttatagacgg gacattccct cagtatgaga gggttatacc tgaagagttt
     4801 atcacatcta tcagggtaga gcgagatgta ctcagagaag ctataaaaag agtcttaccc
     4861 atatcaaggg aggaagatta tgcggttaag tttgctataa agagtagaaa acttatttta
     4921 aacgccaggt ctcctatagg ggttgtcttt gacgaactgg atattgagac tgagggggaa
     4981 gatatagaga ttatcttctc agctaactat atcaccgatt ttctatcggt cggggatggt
     5041 atattaaata ttaagatatc tggtgagaaa gatcccgcca ttttcacatt cgataatgac
     5101 cctgatttta tatatgtgac gatgccaatg gagtcagaat gaaggaggtt tatatactca
     5161 cagagaccat aacattgagc cagtttttaa aatgggcggg tatagctcaa acgggaggag
     5221 aggctaagtt actgatccag caagggggag taactgtaaa taataaggtt gagacacaga
     5281 gaggtagaaa actcagagag aatgatataa tatctgttca gggtcaggga gagtttattg
     5341 ttaagagaaa gtagatatga tacttgattc gatagagctt ctctctttca ggaattttcc
     5401 acatctaatg gtgaatttta atccaaactt taatatattt ataggtccaa atggtgcggg
     5461 aaaaacaaac cttctggagg ctatatatct cttgtcaaac ggtgaactga agaggggtaa
     5521 gagagagatt gaagctataa agtggaacga cccatttacc tggattagag gagaagtaga
     5581 atatgaagat ggaaggctta tgaaacttgt aactcagata agaattgagg gggggaagga
     5641 cttctctata aatggtaaga tagtctccct ccgtagatat ctgggttcct tccctacagt
     5701 ctctatattt gatgatgatc aggatataat caccggagga cctgatagga ggaggaaatt
     5761 cctggatgat tttgttataa gtatagaccc tgagtattat tctatactga atagctataa
     5821 gggtatcctt accaggagga ataaagtatt aaggactaaa aatacagata agaaactgtt
     5881 agaagctctc accgaattat tgctggatta tggggagaag ataatagcta gcagatataa
     5941 tgcagtatct gtctataata gatatctcga cagggaaggg aaaaaattca gtctggagat
     6001 aagggtggaa atcccatctt ttaaagaaga tattcaaaag agtattcaat tttacaggga
     6061 tagatttaat ctatttagac gtaaagaaga gaccatcggt ataaccctta ttggaccaca
     6121 cagagaggat ataataataa ggataaatgg taaggatagc aggagttttg cctctaatgg
     6181 agagatacag ataatactct ttcttttaaa ggttgcccag tttgaaatta taagggaaac
     6241 taagagttat aaaccggtat tcctgataga tgaattctta ggtagacttg atgataagaa
     6301 cagcagactt attatggatt taatagaaga gaaatctcca cagattttta ccaccatgct
     6361 ctttaaaaga gttgttggta agagtggttc aatttttacc atagataatg gagagattat
     6421 agatgaagaa gctttctaat gtcttattgg agttaccact gcgcaggttt tttattctcc
     6481 ccatagcgtg gaataagatt gtcggtaaag aggtggcaag atttgctcat gtgacgtact
     6541 ataaggatgg agtgctatat ataggtgttc cggcagctgt ctgggctaat gagctttcta
     6601 cccactcacc aaagattata gaagagatga gtaaagagac aggtatacct attaccgaga
     6661 taaggttcag ggtatacccg ttctatatca gagaagaaaa ggaattaaac ctagatgaac
     6721 ttactgagga ggataagaaa tatatagatt ctatagtggc aaatgtaaag tatgaaagga
     6781 taagggagtc cttatttaaa ataatagggt atcacatctt aagaacaaga cacggtatat
     6841 aactctctgt tctttatccc ttcatacctg tagtggctat gccttcagta agatattttt
     6901 ggaaaaagat aaatataagg aggaccggca ttagcgatag taccgacatg gcaaatactg
     6961 ctccccagtt agttatagtg gttggatctg caaaattctt taatgccaga gataccgtat
     7021 acagggtagc ctgatttagg tagagcagag gtcccagaaa gtcatcccag gtccagtaga
     7081 atgagaagag actggcagta a
//
LOCUS       DAOTKU010000024         6918 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_10463_length_6918_cov_3.871630, whole genome shotgun sequence.
ACCESSION   DAOTKU010000024 DAOTKU010000000
VERSION     DAOTKU010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6918
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10463_length_6918_cov_3.871630"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="P5310_01170"
     CDS             <1..666
                     /locus_tag="P5310_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HRU32006.1"
                     /translation="TARSILPLVFTLIFFQLVILKKPIENVKEFSIGLLLSIFGLHLF
                     LKGISMSIFPLGDSVGENLVVLERKWLIIIITFVIGVFSTLVEPALKALALEVEEISI
                     GAISNRILVYAVAIGVGTGMSIGVYKTLNNIPYVKVVLPLILLITVLIYFSPEEFISI
                     AMDSASATTGPVNVPLITALSVGLAKILEGVDPLLFGFGTVGLTSIGVVISVLVLGIL
                     TRI"
     gene            684..1007
                     /locus_tag="P5310_01175"
     CDS             684..1007
                     /locus_tag="P5310_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HRU32007.1"
                     /translation="MDIKCIVAIVERGKADKIVDMAKKAGAKGATILYGRGTGQAEAL
                     EFFNIHIEASKEIILILSEIDKYKPIYDTIIKVGRLNEPGTGIIFTFLVSELVGLHHR
                     EQIKD"
     gene            complement(1039..2868)
                     /gene="glmS"
                     /locus_tag="P5310_01180"
     CDS             complement(1039..2868)
                     /gene="glmS"
                     /locus_tag="P5310_01180"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HRU32008.1"
                     /translation="MCGIVGYLGKKKAVPILIDNLKRLEYRGYDSAGVAIIEDSSLIV
                     EKSVGKLSKLESIVIPKDFPSTIGIGHTRWATHGKPSDINAHPHTDCKGEIALVHNGI
                     IENYTDLKDELISEGHRFLSETDTEVIVHLVEKYYAGDLLEAVRMAIKRLEGSYAIAV
                     ISSKEPEKIVVARKDSPLVLGIGEGEYFVASDIPAVLNYTRNIWILENGEMGELSPSG
                     CKVYDLDGNEKKKETMVVSWDISMAEKGGYPHFMIKEIHEQPNALRETFRGRVGSDGL
                     VKLDLKSVTIDDLRNLRKLYFVACGTAYHAGLVGKYLTERFVRIPVEADIASEFRYRD
                     PIFEKNSLMIVISQSGETADTLAALRMAKENGVKVVAICNVVGSSIARESDDVLYTYA
                     GPEIAVASTKAYTSQLMVIYLFMLLLAQIKGTLPLDKIKEFVEDLRKIPEVASEVLNK
                     LEPAIIDLSRRYYKAHDVFFIGRGLDYALSLEGALKLKEISYIHAEAYAAGELKHGPL
                     SLLEEGVPVVCLATQGYLLDKMVSNIKEVKAREAIAIGFGIEGNEGLKNVCDETFFIP
                     KINDIYASVITVIPLQLLAYYMAKERGCDIDQPRNLAKSVTVE"
     gene            complement(2884..3120)
                     /gene="mtrB"
                     /locus_tag="P5310_01185"
     CDS             complement(2884..3120)
                     /gene="mtrB"
                     /locus_tag="P5310_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp RNA-binding attenuation protein MtrB"
                     /protein_id="HRU32009.1"
                     /translation="MAENSDWRLADFIGIKALENGVTIIGLTRGKETRISHTEKLDAG
                     QVMFAQFTENVSAMKIEGKAEIYTKFGKITSGDI"
     gene            complement(3138..3722)
                     /locus_tag="P5310_01190"
     CDS             complement(3138..3722)
                     /locus_tag="P5310_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit A"
                     /protein_id="HRU32010.1"
                     /translation="MFSHLLLLFIGALLVNNILLIRFIALCAFFGVSNSMSTSIGMGM
                     AVTFVMVLTSSVTWILWNFVLVPLHLEYLQISVFILTIAALVQFVELFLKKNSPSLYR
                     AMGIYLPLITTNCAILAVAFIHVSSNFNFIETIVYTIGVSGGYTLAIVMFAGIRERLA
                     SAPVPRAFQGYPLAFITASLMSLAFLGFKGLFGL"
     gene            complement(3722..4312)
                     /locus_tag="P5310_01195"
     CDS             complement(3722..4312)
                     /locus_tag="P5310_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005969446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HRU32011.1"
                     /translation="MKFLRPLINGIWKENPILVLMIGLCPTLAVSTAAINGIYMGLAA
                     TFVLAGSNLIISLIRKWVPDQVRIPIFITIIAAFVTVVDMVMHAYVPEMYKILGIFIP
                     LIVVNCIILGRAEAFAYQNGPIESLGDGIGMGIGFTLILLILGSIRELLGNGTIFGFQ
                     IMPVTYKPVLLMILPPGAFLTIGFLQALQRYRGGKE"
     gene            complement(4309..5235)
                     /locus_tag="P5310_01200"
     CDS             complement(4309..5235)
                     /locus_tag="P5310_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HRU32012.1"
                     /translation="MDQKLYVSAPPHIRSDTDAKFLMRQVIIALLAPTVAGVIFFGGR
                     ALLILVISVLSSIFWQRLMIYIKKEKRPLDGSEVVTGLLIGLNLSPLVPWWIPVVGTF
                     IAIVLAKELFGGLGFNIFNPALIARAFLLASWPMEMTTWAAPFTGVTQATPLALLKLQ
                     HVSTPYISLFLGNRGGCIGETSVLALLIGAAYLFYKGVIDWRTPIPYILTVGVLSLIF
                     GQDPLFHMMSGGLVLGAFFMATDYTTTPVTKSGRIIFGIGCGVITILIRLFGGYPEGV
                     SYSILFMNAMTPLIDRWTIPKPFGYRVERRRV"
     gene            complement(5251..>6918)
                     /gene="nuoF"
                     /locus_tag="P5310_01205"
     CDS             complement(5251..>6918)
                     /gene="nuoF"
                     /locus_tag="P5310_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HRU32013.1"
                     /translation="IGTCDLGPVAIVYPDGIFYVHLKPEDAKVIVNEHLLKGRVVNRL
                     LYQQPMTKEVFPSLSEIDFIVRQKRIVLRNMGVIDPESIEEYIAKEGYQALAKALTMS
                     PEDVIDTVKKSGLRGRGGAGFPTGLKWELTRKATGNPKYVICNGDEGDPGAFMDRSVL
                     EGDPHSIIEGMMIAGYAVGASKGFFYVRAEYPLAIKRIENALEQARSYGLIGENILGT
                     DFSFDIELRRGSGAFVCGEETALIASIEGGRGEPRPRPPYPANRGLWDSPTVINNVET
                     LANISPIILNGAEWFSSTGTEKSKGTKVFALAGKVNNTGLIEVPFGTTLGEIIFDVGG
                     GIPGGKKFKAAQTGGPSGGCIPAEYLDLPIDYESLKQIGAIVGSGGLIIMDEDTCMVD
                     VARFFLEFTQSESCGKCPPCRVGTRKMLDILERIVNGEGEEGDIERLIELGEEIRRDS
                     LCGLGQTAPNPVLSTIRFFRDEYEAHIREKRCPAKSCRALIAYQVIPDKCRGCGICAR
                     VCPVGAAQGKPREIYHIDTGLCIKCGDCFAKCPFGAIEKADLVLEKV"
BASE COUNT         2079 a         1598 c         1188 g         2053 t
ORIGIN      
        1 actgcaagaa gtatacttcc cttagttttc actctgattt tctttcagct ggtgatatta
       61 aagaaaccca ttgaaaatgt taaagaattt agtataggac ttctgctcag tatatttggt
      121 ttgcatctct ttctaaaggg tatttccatg agtattttcc ctttagggga ttcagttggg
      181 gagaatttag tagtattaga gagaaaatgg ttgataatta ttataacttt cgtaataggt
      241 gtattcagca ctcttgtaga accagctcta aaagcactgg ctttggaggt tgaggagata
      301 tctataggag ccatatctaa tagaattcta gtttacgctg ttgccatcgg cgttggtact
      361 ggaatgagca taggggtata taaaacgttg aataatatac cttatgtaaa ggttgtgctc
      421 cctctaattt tattgattac cgtgttaatc tatttttctc cagaggaatt tatatccatc
      481 gcaatggatt cagcaagcgc aacaacgggt ccagtaaatg ttccactgat cacagcttta
      541 tcggtgggac tagcaaaaat actggaagga gtcgatcctc tgctgtttgg ttttggcaca
      601 gtaggactta cttctattgg agtagtaata tcagtgttag tattaggaat acttacaagg
      661 atataatagg aggatggagt tatatggata ttaaatgtat agttgctatt gtcgaaagag
      721 ggaaagcaga taaaattgta gatatggcaa aaaaggcagg agcaaaagga gctactattc
      781 tttatgggag agggacagga caagcggaag cattagagtt ttttaatatt catattgaag
      841 catcgaaaga aatcatcttg atacttagcg agattgataa atataaaccc atatatgata
      901 ctattattaa agtaggtagg cttaacgaac caggtaccgg tatcatattt acttttttag
      961 tgtctgaact ggtagggctg caccataggg aacagataaa agactgaatt ctattgacct
     1021 acctaccccc caataatatt attctacagt aactgacttt gccaagtttc ttggctggtc
     1081 tatatcacaa cctctctcct ttgccatata ataggcaagg agctgaagtg gaatcacagt
     1141 aattacagag gcatatatat cgtttatctt gggaataaag aatgtctcat cacatacatt
     1201 ttttagtcct tcattcccct ctattccaaa accaatggcg atagcctctc tggcttttac
     1261 ctcttttata ttgcttacca tcttatccag cagataacct tgagtagcca ggcataccac
     1321 agggacacct tcttctaaaa gtgataatgg accatgtttt aattctcctg cagcataagc
     1381 ctcggcatga atataagata tttctttaag ctttaacgcc ccctccaggc taagggcgta
     1441 atccaaacct cttccaataa agaatacgtc atgagcctta taatatctcc tggagaggtc
     1501 tataattgca ggctccaatt tatttaaaac ctcactggct acttcaggta ttttcctgag
     1561 gtcttcgaca aactctttaa tcttatctag tggtaaggtc ccttttatct gtgctagaag
     1621 tagcataaac agatatatca ccataagctg cgaggtatat gctttggtgg atgcaacagc
     1681 aatctcaggt ccagcatatg tatacagaac atcatcagac tctctggcga ttgaacttcc
     1741 aaccacatta catatcgcca cgaccttaac tccgttctcc ttagccatac gtaatgctgc
     1801 cagggtatct gctgtttctc cagactgact gataacaatc attaagctat tcttttcaaa
     1861 aattgggtct cggtatctaa actcacttgc tatatctgcc tctactggaa ttcgaacaaa
     1921 tctctctgtt agatatttac ctacaagtcc tgcatgatag gctgttccac aggcaacaaa
     1981 atagagtttt ctaagatttc tcaagtcatc aatagtaaca cttttaaggt cgagtttaac
     2041 cagaccatca cttccaaccc ttcctctaaa tgtttctctc agcgcatttg gttgttcatg
     2101 gatctctttt atcataaagt ggggatatcc acctttttct gccatagata tatcccacga
     2161 tacgaccatt gtctctttct tcttctcatt gccatccaga tcatatacct tacagcctga
     2221 aggagaaagc tctcccatct cgccattttc caagatccat atattacggg tatagtttaa
     2281 gactgctggt atatctgaag ctacaaagta ttccccttca cctattccaa gcactaatgg
     2341 gctatccttt ctggcaacta caatcttttc tggctcttta gaagaaatca cagctatagc
     2401 ataagaacct tccaaccttt taatcgccat tcttacagct tccaggaggt ctccagcgta
     2461 gtacttctct accagatgaa ctatcacttc tgtatctgtt tcagagagga acctgtgacc
     2521 ttctgatata agctcatctt tcaaatctgt ataattttct atgatcccat tgtgaaccag
     2581 ggctatctca cccttgcaat cagtatgtgg atgtgcattt atatctgaag gtttaccatg
     2641 agttgcccat ctggtatgcc ctataccaat agtggaagga aaatcctttg ggatcactat
     2701 agattctagt tttgataatt tacccacact cttctccaca ataagagatg aatcttcgat
     2761 tatcgctact cctgctgaat cgtaccctct atactccagt ctcttcagat tgtctataag
     2821 tatagggaca gcctttttct tacctaaata accaacaatt ccacacatct aaaatttcct
     2881 cctttagata tctccgctag ttatcttccc aaacttggtg tatatctcag cctttccttc
     2941 aatcttcata gctgaaacat tctcagtaaa ctgtgcaaac ataacctgcc cggcatcgag
     3001 tttctctgta tgactgattc tggtttcctt tcctctggta agaccgatta tcgtaacccc
     3061 gttctccagg gcttttatcc caataaaatc tgctaatctc caatcactat tctcagccat
     3121 ttctattctc ctcctttcta tagtccaaac aaccccttaa atcccaaaaa agccagagac
     3181 ataagacttg cagttataaa ggcaagaggg taaccctgga atgccctggg tacaggtgca
     3241 gaagccaatc tctcccttat acctgcaaac ataacgatgg caagggtata acccccactc
     3301 acacctatag tatatacaat cgtctctata aaattaaaat tacttgagac atgtatgaaa
     3361 gctactgcaa gtatagcaca gtttgtggta attagaggta gatatatacc catagcccta
     3421 tataacgaag ggctattctt cttaaggaat aattcaacaa actgtactaa agcagcgatg
     3481 gtaagaataa atacggatat ctggagatat tcaagatgaa gaggtacaag aacaaaattc
     3541 cagagaatcc aggttacaga tgatgtgagg accataacaa atgtcactgc catacccata
     3601 cctatcgatg tactcatcga gtttgataca ccaaagaaag cgcataaagc tataaatctt
     3661 ataagtagta tattatttac cagtagcgcc cctataaata agagaagaag atgagaaaac
     3721 attattcttt acctcctcta tatctctgta gagcctgtaa gaaaccaata gttagaaagg
     3781 caccaggagg aagtatcatt agtaatacag gtttataagt tactggcata atctgaaatc
     3841 caaaaattgt tccattacct aacagttctc ttatactacc aagaatcagt aaaataagag
     3901 taaaacctat ccccatacct atcccatccc ctaaagattc aataggtcca ttctggtagg
     3961 caaaggcttc tgccctgcca agtattatac agttaacaac gattaatgga ataaaaatcc
     4021 ccagtatttt atacatctca ggaacatagg catgcataac catatccact acagtaacaa
     4081 aagcggctat tatggtgata aagataggta tacggacctg atctggaacc cattttctaa
     4141 taagggatat tatcaggttt gaccctgcca aaacaaaggt ggctgctaat cccatatata
     4201 taccatttat agccgcagtt gagacagcca gtgtcggaca gagtcctatc ataaggacaa
     4261 gtatgggatt ttctttccat attccattta tcagaggacg aagaaacttc atactctcct
     4321 cctttcaacc ctatagccaa aaggcttagg gatagtccag cggtctatta agggtgtcat
     4381 agcattcata aagagaatac tgtaagatac accctcagga tatcctccaa agagccttat
     4441 aagtatagtt ataactccgc accctatacc gaagattatc cttccagact tggtaacagg
     4501 ggtggttgta taatcagtag ccataaaaaa tgccccaagg acaagtcctc ctgacatcat
     4561 gtgaaacagc gggtcctgtc caaaaataag ggataagaca cccacagtaa ggatatatgg
     4621 aatcggtgtc ctccaatcaa taaccccttt ataaaaaaga taagcagcac caataagtag
     4681 tgccagaaca gaggtctctc caatacaccc tcctctatta cccaggaaaa gactgatata
     4741 aggggtagat acatgttgta actttaaaag tgctaaaggt gtagcctgag ttaccccagt
     4801 aaatggagca gcccaggttg tcatttccat aggccaggag gctaaaagga atgctctggc
     4861 tataagtgcc ggattaaaga tgttgaaacc cagaccgcca aaaagttctt tagccagcac
     4921 tatagctata aatgttccca ctacaggtat ccaccatggc accagtgggg agagattaag
     4981 accaatgaga agtcctgtta ccacctcact gccatctaag ggtctctttt ctttcttgat
     5041 gtatatcatc aatctctgcc aaaaaattga actcaacaca gatataacta aaatcaggag
     5101 agccctgccg ccaaaaaata tcacacccgc tacagttgga gctaatagag ctattatcac
     5161 ctggcgcatg agaaattttg catctgtatc agacctgata tgtggtggag cagatacata
     5221 cagtttctga tccataggtc tactcctttc ttaaactttt tctaaaacaa gatctgcctt
     5281 ttctatcgct ccaaaaggac acttggcaaa gcagtctcca cactttatac ataatcccgt
     5341 gtctatatga taaatctctc tgggtttacc ctgagctgca cctaccggac aaaccctggc
     5401 acatatacca catcctctgc acttatccgg tattacctga tatgcgataa gagcccgaca
     5461 agatttagca ggacatctct tctctctaat atgagcctca tactcatccc tgaagaatct
     5521 tatagtactc agcacgggat ttggagcggt ctgaccgaga ccacacaatg aatctcttct
     5581 tatctcttca cctaactcta tcagtctttc tatatctccc tcttcccctt caccatttac
     5641 tattctctcc aggatatcca gcatctttct tgtccctaca cgacatggtg gacatttacc
     5701 acaggactca ctctgagtaa attccaggaa gaatcttgcc acatcaacca tacaggtatc
     5761 ctcatccata atgataagac cacctgaacc tactatagct cctatctgtt ttaaagactc
     5821 ataatctata ggaagatcta gatactctgc aggtatacag ccaccagatg gtccacctgt
     5881 ctgggctgcc ttaaattttt tcccacctgg tatcccaccc ccaacatcaa agataatctc
     5941 tccaagtgta gttccaaatg ggacttcaat aagaccggta ttattgacct tacccgcaag
     6001 ggcaaaaacc tttgtcccct tactcttctc tgtacctgta ctgctaaacc attcagcacc
     6061 atttaagatt atgggagaaa tattagccag tgtctccaca ttatttatca ctgttggact
     6121 atcccataat cctctgttag ctggataagg tggacgtgga cgtggctctc ctcttccacc
     6181 ttcaatggag gcgatgagag ctgtttcttc tccacataca aaagcacctg accctcttct
     6241 aagctctata tcaaaagaaa aatctgtccc cagtatattc tctccaataa gtccataact
     6301 cctcgcctgt tcaagagcat tctctatcct ctttatagcc agcggatact cagctctcac
     6361 atagaagaat cctttactgg cccctaccgc ataaccagcg atcatcatcc cttcaataat
     6421 gctatgaggg tcaccttcca gtacacttct atccataaat gcacctgggt ctccttcatc
     6481 accattgcat attacatatt tgggattacc ggtcgcttta cgggtaagct cccattttaa
     6541 tccggtaggg aaacctgccc cacctcttcc tctcagtcct gactttttaa cagtgtctat
     6601 aacatcttca ggactcatag taagagcttt agccagagcc tggtatccct ctttagctat
     6661 atactcttct atgctctccg ggtctataac ccccatattt cttaaaacta ttcttttctg
     6721 tcttactata aaatctatct ctgaaagtga tggaaatacc tctttagtca taggttgctg
     6781 ataaaggagc ctattaacca ccctgccttt tagaagatgc tcattaacta taaccttggc
     6841 atcttctggt ttaagatgga cataaaagat tccatctgga tatacaatag ctaccggacc
     6901 caaatcacag gttcctat
//
LOCUS       DAOTKU010000025         6896 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_10500_length_6896_cov_3.593480, whole genome shotgun sequence.
ACCESSION   DAOTKU010000025 DAOTKU010000000
VERSION     DAOTKU010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6896
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10500_length_6896_cov_3.593480"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2127
                     /locus_tag="P5310_01210"
     CDS             <1..2127
                     /locus_tag="P5310_01210"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HRU32014.1"
                     /translation="NAFIPYSIDITGIIREGENELLVYLDVGKRRVKDKPLTKYGVGE
                     DYYRIWMRKPQFVFGWDWGPYLPTCGIWRPVELNSYESSAIRDVYIRPTILGNKARLE
                     IDVEMENITSEPFELDLSLSLKGDGEYGFGERFTLNPGINKKNYSLEVENPRLWFPQP
                     LGEPYLYSFKATISDKAKELDSYKCNYGIREVKLIQEPMDEGESFIFSINGKEVFCKG
                     ADWVPADSLIANVSREKYKKLVELARSCNFNIFRVWGGGIYEDPYFYELCDRNGIMVW
                     QDFMFACAPYPDDDSEFMEEVARETATIIKELRNHSSLVLWCGNNENQWIHYMGAWGG
                     RDTRLYGTKIYDELLPGLVKELDPTRPYWPSSPYGGEDPNSELCGDRHAWQLSLQIDD
                     PYERINYRLYSLDKGKFISEFGQLAPPLKRSLEEFTPEKELYLDSPTWKFHNNTAERG
                     NIKAALERFFIPQEDLSLDEYLISGQMIQAEALKFALELWMYSDCWGTTGGWTVVDYY
                     LRKKPSYYYVRKAYEPVHISFKERDTYLDVIVTNEIYSPLTLDVEYGVRSFDGAVLLK
                     GKGKLDLSDAGPVRVGEIDASKVPGTYKNRSFVYAKLYYRGRCISLNRTFLVNFKELE
                     IPAVKIVPVLSLIRDGEYQLKLWSDSFAWFVSIDCPDNVELSDNYFDIFPGEEYTVTL
                     RAPGGIELGDISISSLNETVIRHHRV"
     gene            complement(2139..3545)
                     /locus_tag="P5310_01215"
     CDS             complement(2139..3545)
                     /locus_tag="P5310_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32015.1"
                     /translation="MKVTCILLALLFLLVSVTPLLAAEDFNNLSLTIYNQNIALIREV
                     REVVLDAGKQIIKLTDIPSKINPTSVQLNALTDPDSFSVLEQYYKYEPLNSSTLLNKY
                     LGEEIRILTRDGNLYEGYLSNYDVNQIILVKDKKAGPVYVIERSDIVSIEFPQIPEGL
                     ATEPALVCYVESKDAGSKLIELSYLTQDINWSTNYVANISQDEEYLSLNGWVTVENRS
                     GASYESGKLKLIAGEINLEREEYDRADTLKALVAESAPQFEEAGLFEYHIYTLGRGVT
                     LEDNQTKQISLLNAQGIPIEKIFIYEGAAYRGYYYDNWRNQSYNPDVSVYIEFDNRDE
                     YGLGIPLPKGKVRVYIADEDDILQFIGEDSINHTPKDEKIRLSLGSAFDIVGERRIVD
                     HKKISNTIYQDTYEITLKNHREEPAKIKVIERQWGDWKVLKSNSDYTKIDAYNIEFNV
                     EVPENDEVKIVYTAEYRF"
     gene            complement(3574..4425)
                     /locus_tag="P5310_01220"
     CDS             complement(3574..4425)
                     /locus_tag="P5310_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HRU32016.1"
                     /translation="MGLIFNIFWLLIILQMFVPLFQKRMIQLRRQMAIKALEAKRHSR
                     VITMIHRQETMSLLGFPVSRYIDIEDSEYILRAIRMTPPDMPIDIILHTPGGLVLASE
                     QIACALKRHKGKVTVFVPHYAMSGGTLISLAADEIVMDPNAVLGPVDPQLGSSQGGYY
                     PASSILKALEEPNPNRDDQTLILGDIARKAINQVYNTVYSLVRENVPEDKARELAKIL
                     SEGRWTHDYPINYEEAEKLGLPVTDEVPPEVYDLMDLYPQTTQRRPSVEFIPVPYLPP
                     SRKAGEK"
     gene            4533..5519
                     /locus_tag="P5310_01225"
     CDS             4533..5519
                     /locus_tag="P5310_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRU32017.1"
                     /translation="MQYRRLGWTDLKLSVIGFGSWALGGGGWRFSWGPQDDEDSIKAI
                     RRAMELGVNWIDTAAVYGLGHSEEIVGKAIKDLSERPIIATKCGRVWDENGNIRGELS
                     RKSLKRQVDDSLRRLQVDVIDLYQIHWPDPDEYIEEAWSTMADLVKDGKVRYIGVSNF
                     SVEQMKRIEKIHPIASLQPPYSMLRREVESEILPYCKEKNIGVVVYSPMQKGLLTGKY
                     NKETVKQLAPDDHRRTDPMFNEPQLSINVELVEKLKGLAEKYGRTTSQLAIAWILRRP
                     EVTSAIVGIRRPSQIEDVVPAADWNLSEEDIRLIEVWLKEREEKIKNLGVII"
     gene            5516..5890
                     /locus_tag="P5310_01230"
     CDS             5516..5890
                     /locus_tag="P5310_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensory rhodopsin transducer"
                     /protein_id="HRU32018.1"
                     /translation="MSEEIGEYIWLIPDAYLPPYGDLELPSHESTCILNVNKNPAKIK
                     FTFYFEDQDPIESKEIIIPGERTLHVRLDRSKEILGVELKVDVPYSIKVESDTRVIVQ
                     HSRLDTRQANLAYMSSMGYPVR"
     gene            6114..>6896
                     /locus_tag="P5310_01235"
     CDS             6114..>6896
                     /locus_tag="P5310_01235"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011993707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HRU32019.1"
                     /translation="MGGKIFFENERLKIINDDILSTNEIMEGSIDLIVTSPPYNVNIK
                     YNSHNDNLSYEEYIEFTKGWMRKCFNWLKKDGRFCLNIPLDKNKGGQQSVGADLTVIA
                     KEIGFKYHSTIVWNEGNISRRTAWGSWVSASAPYVIAPVELIVVLYREEWKKTSGSGI
                     SDINREEFMEWTNGLWTFPGESKKKVGHPAPFPVELPKRCIKLFSFVDDLVLDPFMGS
                     GTTLIAAHLTGRRAIGVEIDQSYCEIAKKRLIKEAGVNKRSLF"
BASE COUNT         2067 a         1194 c         1529 g         2106 t
ORIGIN      
        1 aatgctttta tcccatattc catagatatt acaggtatta tcagagaggg agagaacgag
       61 cttttggttt atctggatgt cggtaagaga cgggtgaagg ataaacctct aacgaagtat
      121 ggggttggag aagattatta ccggatctgg atgagaaaac cccagtttgt atttggatgg
      181 gattggggac cgtatcttcc tacctgtggt atatggagac ctgtcgaatt aaatagttat
      241 gagtcttccg caataaggga tgtttatata agacctacta tactaggaaa taaggctaga
      301 cttgagatag atgttgagat ggaaaatata acatccgaac cctttgagtt agacctttcc
      361 ctttcactta agggtgatgg ggaatatggc tttggagaga gattcacact taatcctggt
      421 ataaataaaa agaactattc tcttgaggta gagaatccca ggttatggtt ccctcagcct
      481 ttaggtgagc catatctata ctcttttaag gctaccatct cagataaagc taaggagctg
      541 gactcttata agtgtaatta tggtataaga gaagtgaagc ttatacagga gcctatggat
      601 gagggagaga gttttatttt ttctataaat gggaaggagg ttttctgcaa gggagcagac
      661 tgggtccctg ctgattcttt aatagccaat gtctccaggg aaaagtataa gaaacttgta
      721 gaacttgcaa ggtcttgtaa tttcaatata ttcagagtat ggggtggggg catatatgag
      781 gacccatact tctacgaatt atgcgatagg aatgggatta tggtctggca ggattttatg
      841 tttgcctgtg caccatatcc agacgatgat tcggagttta tggaagaggt agcaagagag
      901 acagcgacga taataaaaga gctacgtaat cattcctctc tcgtcctctg gtgtggtaac
      961 aatgagaacc agtggattca ctatatggga gcctggggag gtagagatac aagattgtat
     1021 gggacaaaga tatacgatga attacttcca ggacttgtaa aagagttaga tcctaccaga
     1081 ccttattggc cttccagtcc ttatggaggt gaagacccca actcagagtt atgtggggac
     1141 aggcatgcct ggcagttaag tctccagatt gatgatccgt atgaaaggat aaactacagg
     1201 ctctattctc tggataaagg gaaatttatc tctgagtttg gacaactggc accaccttta
     1261 aagagaagtc tagaggagtt tactcctgaa aaggagttat atcttgactc tcctacctgg
     1321 aagtttcaca ataataccgc tgagagagga aatataaagg ctgctttaga gagattcttc
     1381 atacctcagg aggatttatc actggatgaa tatctcatct ctgggcagat gatacaggcg
     1441 gaggctctaa agtttgccct ggaactctgg atgtattctg attgttgggg aactacaggc
     1501 ggatggacag ttgttgatta ttatctcaga aagaagccat cttattatta tgtgagaaaa
     1561 gcctatgaac ctgttcatat ctcctttaag gagagagata cttatctgga tgtaatagtt
     1621 acaaatgaga tttatagtcc ccttactcta gatgtagagt atggagtgag gtcctttgat
     1681 ggggctgttt tactgaaagg gaagggtaaa ttagacctgt cagacgcagg tcctgtcagg
     1741 gttggagaga tagatgcctc caaggtgcct ggaacgtata aaaacagatc tttcgtgtat
     1801 gcaaagctct attatagggg tagatgtata tccctgaata gaacattcct ggtgaatttt
     1861 aaggaattag agattccggc ggtaaagatt gtccctgtac ttagccttat aagggatgga
     1921 gaatatcagt tgaagctctg gagtgatagt tttgcctggt ttgtatctat agattgtcct
     1981 gataatgttg agttatcaga caattatttc gatatattcc cgggagagga atacacagtt
     2041 actctgagag ctccaggagg aatagagtta ggtgatatat cgatctcttc tctcaatgag
     2101 acggtgataa gacatcacag ggtataaaat aatatacgct agaacctgta ctctgctgta
     2161 tagactatct ttacttcgtc gttttcaggg acttccacat tgaattctat gttataggca
     2221 tcgatcttgg tatagtcaga atttgacttt aagaccttcc aatcacccca ctgtcgttct
     2281 ataaccttta tcttcgcagg ctcttctctg tgattcttta atgtaatctc ataggtatcc
     2341 tgatatatag tattgcttat cttcttgtgg tccactatcc ttctttctcc aactatatcg
     2401 aatgcgctac caagtgaaag tcttatcttt tcatccttcg gggtatgatt gattgaatct
     2461 tctccaataa actgtaaaat atcatcttca tctgctatat atactctaac ctttccttta
     2521 ggtaggggaa tacctaaacc gtactcgtct ctgttatcaa actctatata aactgagacg
     2581 tccggattat agctctgatt tctccagtta tcataataat aacccctgta agctgcgcct
     2641 tcatagataa atattttttc tataggaatc ccctgggcgt tcagtagaga tatctgcttg
     2701 gtctgattat cctctagtgt tactcctctc ccaagggtat agatatgata ctcgaagaga
     2761 ccagcctctt caaattgcgg tgcactttct gctacaagag cctttaatgt atcagcccta
     2821 tcatactctt ctctttccag atttatctct ccagctataa gctttagctt gccagattca
     2881 taacttgctc cagatctgtt ttctacagtt acccagccat taagtgatag atattcttca
     2941 tcctgtgata tattggctac ataatttgtg ctccagttaa tatcttgagt taaatagctc
     3001 agctcaatca gcttagagcc tgcatcttta ctctcaacat aacagaccag agctggttct
     3061 gtcgccaacc cttcaggtat ctgtggaaat tctatggata ctatgtcact tctctcaatc
     3121 acatatacag gtccagcctt cttatctttt acaagtataa tttgattgac gtcataattt
     3181 gacaggtacc cctcatatag attaccatct ctggtaagta tccttatctc ctctcctaaa
     3241 tatttattga gcagcgtgga agaatttaat ggctcatatt tatagtattg ctcaaggaca
     3301 gagaaactgt cagggtcggt aagagcgttc agctgaacac tggtgggatt aatcttagat
     3361 ggtatatctg ttaattttat aatctgcttc cctgcatcca gtactacctc ccgtacctct
     3421 ctgataagtg caatgttttg attatagata gttaaagata aattattgaa gtcttcagca
     3481 gccaatagag gtgttacact cactaataaa aacagtaatg ccagtaaaat gcaggtaacc
     3541 ttcataaata tcacctccag tataattttt gccttacttc tccccagcct ttctagatgg
     3601 gggtaagtat ggtaccggta taaactctac agatggacgc ctctgggttg tctgaggata
     3661 caggtccata agatcataca cctctggagg gacttcatct gtaaccggta gtccaagttt
     3721 ttctgcctct tcataattta ttggatagtc gtgagtccat cttccctcac tcaaaatctt
     3781 tgctagttct ctcgctttat cctctgggac gttctctctt accagagaat agactgtgtt
     3841 ataaacctga tttatagcct tacgagctat gtctccaaga attagggtct ggtcatcacg
     3901 atttggattt ggttcttcca gggctttcaa tatagacgaa gctggataat atccaccctg
     3961 agaactgcca agttgtgggt ctaccggacc taagactgca tttggatcca ttactatctc
     4021 atcagcagct agagatataa gtgttccccc agacattgca tagtggggaa caaacacggt
     4081 taccttcccc ttatgccttt ttaaggcgca ggctatctgt tctgaagcca gaactaaccc
     4141 acctggagtg tgaagtataa tgtctatagg catatccggt ggggtcattc taattgctct
     4201 taagatatac tcagaatctt ctatatcaat ataacgactt accggaaatc ctaacaagct
     4261 cattgtctcc tgtctgtgta tcatggtaat tactctgctg tgtctttttg cctccagagc
     4321 tttgatagcc atctgtctcc ttagctgaat catcctcttt tgaaagaggg ggacaaacat
     4381 ctgtaatata ataagcaacc agaagatatt aaaaattaaa cccatagtat ttccctccta
     4441 tccttttatt atctatttta ttacacaaaa tagataacgt caattgtatg ttataattgt
     4501 aatatctttt tggaatagtt ggaggtgtaa ggatgcaata ccgtaggctt ggatggacag
     4561 acctaaaact ttctgtcata ggctttggat cttgggcatt gggtggcggt ggctggaggt
     4621 ttagctgggg tccacaggat gacgaggatt ctataaaagc gatacggaga gctatggagt
     4681 taggggtaaa ctggatagat accgctgctg tttatggctt aggtcattct gaagagatcg
     4741 ttggaaaggc tataaaagat ttgtcagaga gacctattat agctaccaaa tgtggtagag
     4801 tttgggatga gaatggtaat ataaggggtg aactctccag aaaaagttta aagaggcagg
     4861 tggatgatag tttaagaagg ctccaggttg atgtgattga tttatatcag atacactggc
     4921 ctgatccgga tgaatatata gaggaagcct ggagtactat ggcagacctg gtaaaagatg
     4981 ggaaggtaag gtatattggg gtatctaatt ttagtgtaga gcagatgaaa aggatagaga
     5041 agatacatcc gatagcttct ttacagcctc cgtatagcat gctcagaaga gaagttgaga
     5101 gtgagatttt accttactgt aaggagaaga atataggtgt agttgtttat agccctatgc
     5161 agaaggggtt actgactggt aagtataata aagagacagt taagcaattg gctccagatg
     5221 accatagacg taccgacccc atgtttaacg agccccagct aagtataaat gtagaattgg
     5281 ttgaaaaatt aaaggggctt gccgaaaaat atggaagaac aacttctcag ttagctattg
     5341 catggatttt aagacgacca gaagtaacca gtgctattgt tgggatacgt cgtccttctc
     5401 agatagaaga tgtggtacct gctgctgact ggaatctgtc tgaggaagat ataagattga
     5461 tagaagtctg gcttaaagaa agagaagaaa agattaaaaa cttaggagta ataatatgag
     5521 tgaagagata ggagaatata tctggttgat acctgatgca tatcttcctc cttatggaga
     5581 cctggagttg cctagtcatg aatctacctg tatcctgaat gtaaataaaa acccggctaa
     5641 aatcaagttt acattctact ttgaagacca ggatccgata gaatctaaag agattattat
     5701 tccaggtgaa aggactttgc atgtaagact ggacaggtcg aaagaaatcc ttggagtaga
     5761 attgaaggta gatgttcctt attctataaa ggttgaaagt gacaccaggg ttatagtgca
     5821 gcactccagg ctggatacga gacaggctaa tctggcctat atgtccagta tgggttatcc
     5881 ggtcagatag tagtaaaatt taataatatt tatacctgag gataggtaaa tagaggaaga
     5941 gatttctatt tgtttatcct tacttaaatc tctggagttt tagaatatta tcctaggaaa
     6001 gatattctgg acgaacctta tttgagaaca aaatgggatc agggtaatct taagcttgaa
     6061 ggattgctaa atgtctttgc aatgttaata attcctgata taatttaacc aagatgggtg
     6121 gtaagatttt tttcgaaaat gagagattaa agattattaa tgatgatata ctttctacca
     6181 atgagattat ggaaggaagt atagacctta ttgttacctc tccgccatac aatgtaaata
     6241 taaaatataa tagtcataat gataacttat cttatgagga gtacatagag tttaccaaag
     6301 gatggatgag aaaatgtttt aattggctta agaaggatgg tagattctgc ctgaatattc
     6361 ctttggacaa gaacaagggg ggacaacaga gtgttggagc ggatttgaca gtaatagcta
     6421 aagaaatagg gtttaaatat cattctacta tcgtatggaa cgagggaaat atttctagaa
     6481 gaactgcatg gggttcctgg gtaagtgcat ctgctccata tgtaatagct cctgttgaat
     6541 tgatagtggt cttatacaga gaagaatgga aaaagacaag tggaagtggc atatctgaca
     6601 ttaatagaga agaatttatg gagtggacaa atggtctgtg gacatttcct ggtgagagca
     6661 agaagaaggt aggtcatcct gctccatttc cagtagaact ccctaaaaga tgtataaaac
     6721 tatttagttt tgtagacgat cttgtacttg atccatttat gggaagcgga acaactctga
     6781 tagcagctca tcttacagga agaagggcta taggagttga aatagatcaa tcttactgtg
     6841 agattgctaa aaagaggtta ataaaagaag ctggagtaaa taaaaggagt cttttt
//
LOCUS       DAOTKU010000026         6635 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_10939_length_6635_cov_3.842249, whole genome shotgun sequence.
ACCESSION   DAOTKU010000026 DAOTKU010000000
VERSION     DAOTKU010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6635
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10939_length_6635_cov_3.842249"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(156..3338)
                     /locus_tag="P5310_01240"
     CDS             complement(156..3338)
                     /locus_tag="P5310_01240"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HRU32020.1"
                     /translation="MYKVREVTENNLILEGEGRIIHISFPGQEIIRFKVIEEDADETS
                     EFKIAEEKTPERIDAFKISENKLKVSSGPTELFLTLNPLSFKVYKDGKEKLSSDESVF
                     LEKRKEETIFRFKLNSEERIYGLGQDCMANLEQNGQERRCWHQWNGFRRSGNGSIPFL
                     LSSQNYGLFLDTSWPVRFNIGGDKIPEKNLGEDWAPSPWDWDLPSGEGNPNRLSVLIY
                     GSQVADFYILLGEDFNRIIRNYYEVTGTPRLLPKWAYGFIQCKNRYRSEEELLRLGKK
                     FRERGIPCDVIVIDWLWFKEFGDLEWNREYWAHPEETFKELSRLGFKIMQAQHPFVER
                     TSLKYEIFKEKGYLNDVPPSARPTFDHSNPMARKGWWEEVKRLYETGIRGYWTDMGEI
                     EQHKPGTKSYLGSREKVHNIYTLLWTKGLYENQRETFGTRSFSLARTLWCGIQKYGVA
                     VWSGDISSSWDVLKDQVIIGQGVTLSGMPFWCTDIGGFFFHPDFTPELYVRWFQWGVF
                     CPIFRTHGTRPENEPWSFGKESEAILKKFIRLRYKLMPYIYSLAHNTSSGKQMMRAMV
                     VDYPEDKNACESKYQFMFGDILVSPVVEKGARVKETYLPEGYWYDFWTGKRYRGKRFI
                     KTLAPVDRIPLFIRGSAIIPVNGEEVMYADEKPLKTLEINIYTPGNGEFTLYEDDGVT
                     YEYEEGKSVKTRISTFETDSQLRIEISSYGKEFEGFEKDKTFNFNIYGIAARDVSVDG
                     GKTSFQNSKGFLRVEGIRYQFNNKLTIAIEKDKENIVEDKGIEIFVDGLDKGQDGSIG
                     ISIVAMNGGSSPTRKVYFEIDKPGDWAVVSSDLPYGTSSRPYPDGRATLEPWGIYQGK
                     LVISRIGASRSPFEEFTLKAKIETGEEVSEKKFIIELGEGDTSGWKVIGPFDNEIKEI
                     EQNPDKVEYPVGNSLWRWISGDKFIVEPFGYLDIMKLAREVLVKEMLNENITRGTAYA
                     KLEVYSDKDRDVRFSLYGEGKLEFFVDGTSAYKSDGIVYEDEISLSLKEGINRILVKV
                     SFDFPKPYSGREFGFRLRLSEPVEGLLFLE"
     gene            complement(3417..4289)
                     /locus_tag="P5310_01245"
     CDS             complement(3417..4289)
                     /locus_tag="P5310_01245"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32021.1"
                     /translation="MERVKVYCPGSCGELIQGVIYGRELLISYPIELGTVVSIEISEK
                     KDTEKLTPKIESAAEKTLEFLGIPKRFLENIKIRKTSSLPLGKGMSSSTADIASVIFG
                     IGVLFGIELDSHKIAKISVSIEPTDSIIFEDITLFDSLRGEFILPIVPVPSLKVIVLE
                     GKGSVDTLEYHNRTGKQVYSYADEWEEAYSLMDRSLKRGDWNGVGDASIKSALIQQRI
                     LPKPHLHEIIKCALDLGAYGINVAHSGTAVGIFMEERKDGTPIVNRLKEMGILDAYER
                     YHITRMVKGGPRVL"
     gene            complement(4295..5347)
                     /gene="cobD"
                     /locus_tag="P5310_01250"
     CDS             complement(4295..5347)
                     /gene="cobD"
                     /locus_tag="P5310_01250"
                     /EC_number="4.1.1.81"
                     /inference="COORDINATES: protein motif:HMM:TIGR01140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-phosphate decarboxylase CobD"
                     /protein_id="HRU32022.1"
                     /translation="MIEHGGNIYRFARILDREPSTLIDFSANINPLGFPEGLKEHLFS
                     LMDYITSYPDPEYVELRDAIAGYVGVRKENIIPGNGAIEMIYLYMKSMKKDKVFIPVP
                     TFSEYERIARMNSLAIEYLFTKNFSIDVERLVKEISPESILIICNPNNPTGTLISIDS
                     IEYLLEKLSGLEISLMIDEAFIEFTEDYPESSAVRFINSYPNLFIVRCFTKFFGIPGL
                     RLGYGVGGEETVNKMKEFALPWSVNILADISGRYVLKNMSFMEETRRVITLEREFLLR
                     ELSNIKWIYPYPGKANFILARLSIDTEELENFLLQRGILIRNCRNFKGLDKSYIRIAV
                     KDHSSNVKLVEALKSF"
     gene            complement(5344..6285)
                     /gene="cbiB"
                     /locus_tag="P5310_01255"
     CDS             complement(5344..6285)
                     /gene="cbiB"
                     /locus_tag="P5310_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011722155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016880 - acid-ammonia (or amide) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HRU32023.1"
                     /translation="MFDIVMGYIADLIFGDPYWFPHPVRFIGRFISSFENLLRRLVHS
                     ETSEKIAGFILTGVTVVISYLVVWILLKISLSVSTLLFHIVNIFFIYTVLATRCLGDE
                     AKKIYRYLINGDINNARKALSYIVGRDTEKLDVQGICRAVIETVSENTSDGIVAPLFY
                     LFLGGVPLAMAYKAINTLDSMVGYKNERYINMGMASARLDDIANFIPARLTSFLIAIA
                     SFITGLNPRRSLSIMLRDGRNNPSPNSGYPEASMAGALGVRLGGPSSYGGQIVEKPYI
                     GDPEDTVTPEHIILSTRVMYVTSLLALITGLIIQVIL"
     gene            complement(6286..>6635)
                     /locus_tag="P5310_01260"
     CDS             complement(6286..>6635)
                     /locus_tag="P5310_01260"
                     /inference="COORDINATES: protein motif:HMM:NF019305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cobyric acid synthase CobQ"
                     /protein_id="HRU32024.1"
                     /translation="GLSGSTLKGYEIHMGRTEDLGNSIPFSRIIKRGESLCNEIDGYM
                     SCDGNVFGTYLHGIFDNESFLKGLVSLIYRRKGIEKDVDINPVTEEFKYDELARIVRE
                     SVDIQEIYRIMGL"
BASE COUNT         1744 a         1617 c         1112 g         2162 t
ORIGIN      
        1 cccacctcta tcctcccccg aaatagggga ggaagaaaat ggattatggg attatcatca
       61 taaaatgacg aaattgtagg ggcaattcat gaattgcccc tacaggatac aaagtaatct
      121 catatgtttc gttcccttcc cccttatctt cttttttatt ctaaaaacag tagcccttct
      181 acaggttctg acagccttag tctaaagcca aattcccttc cgctgtatgg ctttgggaag
      241 tcaaatgaga ccttaaccag aattctattt atcccctctt tgagagaaag gctgatctca
      301 tcctcgtata cgatgccatc actcttatat gcagaggtcc catcgacaaa gaactccagt
      361 ttaccttcac catatagaga aaaccttaca tccctatctt tatcagaata aacctccagc
      421 tttgcataag cggtccccct ggtgatattt tcatttagca tttccttaac cagaacttct
      481 ctggccagtt tcattatgtc tagatatcca aatggctcga ctataaactt atcccctgat
      541 atccatctcc ataagctgtt tccgactgga tattctactt tgtctggatt ctgctctatc
      601 tcttttatct cgttatcaaa tggtcctata accttccacc ccgaagtatc cccctctcca
      661 agttctataa taaatttctt ctctgatacc tcttctccag tttcaatctt cgccttaagt
      721 gtaaattctt caaaaggaga tctggatgcc cctatcctgg aaatgaccag tttaccttga
      781 tatatccccc agggttctaa cgttgccctg ccatcaggat acggtctgct tgaggttcca
      841 tatggtagat ctgaagatac cacagcccaa tctcccggtt tgtctatctc aaagtagacc
      901 tttcttgttg gagaagaacc tccattcatt gccactatac ttattcctat actcccatcc
      961 tgccccttgt ccagtccatc cacaaagatc tctataccct tatcttcaac tatattttct
     1021 ttatcctttt ctatggctat cgtgagcttg ttgttgaatt gatatcttat cccctctacc
     1081 ctcagaaatc ccttactatt ctggaaactg gttttccctc cgtctaccga tacgtctctg
     1141 gcggctatgc catatatatt gaagttaaag gtcttatctt tttcaaatcc ttcaaattct
     1201 ttgccgtaac tcgatatctc tatccttaat tgactatccg tctcgaatgt cgatatcctg
     1261 gttttaacag atttaccctc ctcatattca tatgtgactc catcgtcttc atacagggta
     1321 aactccccgt tacccggggt gtatatgttt atctccaggg ttttaagagg tttttcatcc
     1381 gcatacataa cctcttctcc atttacaggg attatggctg aacctcttat aaacagaggt
     1441 atcctgtcta caggtgcgag agtttttatg aacctctttc ccctgtacct tttgcctgtc
     1501 cagaagtcgt accagtaacc ctcaggtaga tatgtttcct ttaccctggc acccttttct
     1561 acaacaggtg aaacaaggat atctccaaac ataaactgat atttactctc acaggcattc
     1621 ttatcctccg ggtaatctac aaccatagcc ctcatcatct gctttccact ggaggtgtta
     1681 tgtgccaagc tgtagatgta aggcataagc ttatatctta accttataaa cttcttaagg
     1741 attgcctcag actcttttcc aaatgaccag ggttcatttt ccggtctggt tccgtgagtc
     1801 ctgaagatag ggcagaatac tccccactga aaccatctta cataaagttc aggggtaaaa
     1861 tcaggatgga agaagaagcc tccaatatct gtgcaccaga atggcatacc actcagggtt
     1921 actccctgac cgatgattac ctggtccttc aagacatccc agcttgagct tatgtctccc
     1981 gaccacacag caacaccgta tttttgtatc ccacaccata atgtcctggc cagggagaaa
     2041 cttctggtcc caaatgtctc cctctgattc tcgtagagac ctttagtcca tagaagtgtg
     2101 tatatattgt gcactttttc tctggagccc agataactct ttgtaccagg cttatgctgt
     2161 tctatctcgc ccatatctgt ccagtaacct cttatccctg tttcatacag cctttttacc
     2221 tcttcccacc agccttttct cgccattgga ttagaatggt cgaatgttgg tctggctgaa
     2281 ggtggcacat cattcagata ccccttctct ttaaatatct catactttag agatgtcctc
     2341 tctacaaatg gatgctgtgc ctgcataatc ttgaatccaa gtcttgacag ctccttaaat
     2401 gtttcttctg gatgtgccca gtattctctg ttccactcaa gatcgccgaa ctctttgaac
     2461 cagagccagt caattacaat gacatcacag ggtatacctc tttccctgaa ttttttcccc
     2521 agtctgagca attcttcctc actcctgtat ctatttttac actgtataaa tccataagcc
     2581 cacttgggga ggagtctggg agttccggta acttcatagt agttcctgat tattctgttg
     2641 aaatcttcac ccagaaggat gtagaaatct gcaacttgtg acccatagat taatacagaa
     2701 agcctgttag gattaccttc accacttggc aggtcccagt cccagggaga tggtgcccaa
     2761 tcttctccaa gatttttttc agggattttg tctcccccta tattgaacct taccggccag
     2821 ctggtatcta aaaatagccc ataattttgg gatgatagaa ggaacgggat agaaccattc
     2881 ccacttcttc tgaaaccgtt ccactgatgc caacaccgtc tctcctgccc attctgttcc
     2941 agattagcca tacagtcctg tcccagacca tagattcttt cctctgaatt cagtttaaat
     3001 ctgaagatcg tctcttcttt tctcttttct aagaatactg attcgtcact ggatagtttt
     3061 tcttttccat ctttatagac cttaaaagat agcgggttaa gggttagaaa cagttcagtg
     3121 ggacctgatg agacttttaa cttattctct gatattttaa aggcatctat cctttctggg
     3181 gtcttttctt ctgctatctt aaattcactg gtctcatctg catcctcctc tatgaccttg
     3241 aatctgataa tctcctgacc tggaaaagag atatgtataa ttcttccctc accctctaaa
     3301 atcaggttat tttcggtaac ttctcttacc ttatacatta gatcacctct tttgataagt
     3361 ttatgatttt ataaaactat tatagggata aaataataat atatcaattt acgcttctat
     3421 aaaactctgg gtccaccttt taccatcctg gttatatggt acctctcata ggcatctaat
     3481 atccccatct ctttcagtct gtttacgata ggagttccat cttttctctc ttccataaat
     3541 atacccacgg ctgtaccgct gtgggctaca tttattccgt atgccccgag gtccagagca
     3601 cattttatta tttcatggag atgaggtttt ggaagtatcc tctgctgtat cagtgcgctt
     3661 tttatggatg catctccaac gccattccag tcccctctct ttagagacct atccatcaaa
     3721 gagtacgcct cttcccattc gtcagcgtag gagtagacct gtttgcctgt cctattatga
     3781 tactccaggg tgtcaaccga gcctttacct tccaggacta tcactttaag tgatggcact
     3841 ggaactatcg gcaggataaa ttcccccctc agagaatcaa atagtgtaat gtcctcaaat
     3901 attatgctgt ctgttggctc tatagatacg gaaatttttg ctattttatg gctatccagc
     3961 tctatgccga acagaacccc gatcccgaat ataactgaag ctatatctgc tgtactgcta
     4021 ctcataccct tccccaatgg gagagaagag gtctttctga tttttatatt ctctaaaaat
     4081 ctcttgggaa ttccaagaaa ctccagtgtc ttttctgctg ctgattctat ctttggggta
     4141 agtttttcag tatctttctt ttcagatatt tcaatggaga caactgttcc aagttctatc
     4201 ggatatgaga tcaggagttc tctcccataa ataaccccct gtataagctc cccacaggaa
     4261 ccggggcaat agacctttac tctctccata gattctaaaa agactttaat gcctcaacaa
     4321 gttttacatt actgctatgg tcttttacgg caattctgat ataactctta tccagaccct
     4381 taaagtttct gcagtttctt atcaggatac ccctttgcag gagaaaattc tctaactctt
     4441 cagtgtctat ggagagcctt gccaggatga aatttgcctt accgggatac ggatatatcc
     4501 atttgatatt ggagagctct ctcaagagaa attcacgttc cagtgtaatg acccttcttg
     4561 tttcttccat aaatgacatg tttttcaata cgtatcttcc cgatatgtct gctaggatat
     4621 ttacagacca ggggagggcg aattctttca tcttattgac agtctcctct ccgcctaccc
     4681 cgtaacccaa ccttaaccct ggaattccga aaaatttggt gaaacatctt actatgaaga
     4741 gattcgggta tgaattgata aatcttaccg ctgagctttc cggataatcc tcggtgaatt
     4801 ctataaatgc ctcatctatc atcagagata tttcaagccc tgataacttc tcgagcaggt
     4861 attctatgga gtctatgctt atgagggttc cggtcgggtt attgggatta cagattatga
     4921 ggatactttc aggggaaatc tctttgacca gtctttctac atcaatcgag aagttttttg
     4981 taaagagata ttcaattgct aaactattca tcctggctat acgttcatat tcagagaagg
     5041 ttgggacagg tataaatacc ttatccttct tcatagattt catatagaga tatatcatct
     5101 ctatggcgcc attaccaggt attatattct cttttctcac accgacatac cccgctatag
     5161 catctcttag ctctacatat tccgggtcag gataactggt tatgtaatcc atcaaggaga
     5221 ataggtgctc cttcaatcct tctggaaatc ccagaggatt tatatttgca gaaaaatcaa
     5281 tcaatgtaga aggttctcta tccagaatcc tggcaaacct gtatatattt cctccatgtt
     5341 caatcataat atcacctgaa taataagtcc tgttataagt gccagaaggg atgttacata
     5401 catcactctg gtcgataaga ttatatgctc tggtgtcact gtatcttcgg ggtctcctat
     5461 atacggtttt tccactattt gtcccccata actgctcggt ccacccagac gaactcctaa
     5521 tgccccagcc atactggctt caggatatcc actgtttggg cttggattat tcctcccatc
     5581 ccttaacatt atggatagac ttctcctcgg attcaatcct gtaataaaag atgcgatggc
     5641 tatcagaaag gatgttaatc ttgccgggat gaagtttgct atatcatcca gacgtgcaga
     5701 agccatcccc atatttatat aacgttcatt tttatatccc accatagagt ccagcgtatt
     5761 tatcgccttg tatgccatag caagaggaac tcctcctaag aagagataaa ataatggtgc
     5821 aacaattcca tcagatgtat tttcagacac ggtttctata acagccctgc atatcccctg
     5881 cacatcgagt ttttcagtat ctcttccaac tatatatgat agggcttttc tggcattgtt
     5941 aatatccccg tttatcagat atctgtaaat cttcttagcc tcatcaccca gacatctggt
     6001 tgcaaggaca gtatagataa aaaatatgtt gacgatatgg aatagtaatg tgcttacaga
     6061 taaagagatt tttagtaaaa tccagactac tagatatgat ataactactg taacccctgt
     6121 cagtataaag cctgctatct tctctgatgt ttctgagtgt actagacgcc tgagcagatt
     6181 ctcgaaagaa gatataaacc tacctataaa tcttaccgga tgtggaaacc agtacggatc
     6241 accaaatata aggtctgcga tgtaacccat cactatatcg aacatctata gacccattat
     6301 cctatatatt tcctgtatat caaccgactc tctcactatc cttgcaagct cgtcgtactt
     6361 aaattcctct gttacgggat taatatctac gtctttttct ataccctttc tcctgtaaat
     6421 gagagagacc agccctttta gaaagctctc attgtcaaat attccgtgta gatatgtacc
     6481 gaatacattg ccatcacaag acatatatcc atctatttca ttacagaggg attctcccct
     6541 tttaataatg cgggaaaatg ggatagaatt ccccagatct tccgtccttc ccatatgtat
     6601 ctcatagcct ttaagagtag aaccggataa cccgg
//
LOCUS       DAOTKU010000027         6573 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_11045_length_6573_cov_3.738263, whole genome shotgun sequence.
ACCESSION   DAOTKU010000027 DAOTKU010000000
VERSION     DAOTKU010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6573
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11045_length_6573_cov_3.738263"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="P5310_01265"
     CDS             <1..574
                     /locus_tag="P5310_01265"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRU32025.1"
                     /translation="TLVYLPYVLPAVAVGFIWLWMLNPYYGLVNLFLNAVFHIQGPGW
                     FSDPKWAKPAFVMLAQWGAGGSAVIFLSAIKDVPREMLESAEIDGAGWWTKFSKIIIP
                     MISPVILYHIITALFGGFQIFTTAYIATNGAPANSTLFYVLYLYRNAFQYGKMGYASA
                     MAWILFILVLIITLIIFRTSARWVFYQGGE"
     gene            579..1412
                     /locus_tag="P5310_01270"
     CDS             579..1412
                     /locus_tag="P5310_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004101577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRU32026.1"
                     /translation="MNLRWFLSKVVTRLVLILVSALYLVPIFWMFTTSIKDMSQVMAY
                     PPIWWPDPPKWHNYVEAVNYIPFGQYTLNTILICAGNIVGVLFSCPLVAYSISKIKWR
                     GRNFLFMLILSTMLIPFPVTMVPLFVVFARLGWINTFTPLILPAFFGGGAFNIFLLRQ
                     FFLTIPEELSDAARIDGASEFGIYSRIILPLIKPILIVIALFTFLGAWNDFMGPLIYL
                     QDEAKYTLALGLQMYRSTNYVEWPLLMAAATIVVIPTIIAFYFTQNTIVESVTLTGLK
                     G"
     gene            1418..3724
                     /locus_tag="P5310_01275"
     CDS             1418..3724
                     /locus_tag="P5310_01275"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HRU32027.1"
                     /translation="MSFPCKILISLLIFAFLFPLPCAGGEDLFVKYVDPFTGTGGHGH
                     TFPGVTVPFGMVQLSPDTGVEGWDWCSGYHYSDSSIMGFSHTHLSGTGAADYGEVRVM
                     PVVGDLKIIPGPKDNPNIGYRSRFSHKKEIARPGYYSVFLDDYKVQAELTASQRVGFH
                     RYTFPKTDKGYIIFDLYNRIGDMAESAWVKIVGNDEIEGSITGGHFCGARDPQTIYFV
                     AKFSKPFKEFGTWKVFRVDKGNREEKMLNRSEPFIGAYVRYSTSEKEKILIKVAISYT
                     GVDGARKNLQEIPDWNFDRVRENAEKLWEKELGKIEVEGNESDKTKFYTALYHSFIHP
                     SLFSDIDGKYIGTDDKIYSSTYDHYTVFSLWDTFRSLHPLFLLIQPQKNTDMIKSFLD
                     IYSQSGWLPRWHKGNRETNCMIGTHSDSVIADAIIKGLRDFDLEKAYSAMYKNAMVES
                     MDFNQGRRGITEYKKSGYVPGDKFDQSVSRTLEYAYNDFCLAQVSKVLGKEEDYKLFI
                     SRAQNYKNLYDSNVGFMRPRDSNGNWISFKTSFSSSFDPTDATSPHYTEGNAWQWTFF
                     APQDPYGLITLLGGEEKFIKKLDEFFNTKSPIKGPPDITGLIGQYAHGNEPSHHISYF
                     YVYARMPWKTQEMVRKIVDSLYGIGPEGLCGNEDCGQMSAWYVFSSLGFYPFCPGTPV
                     YILSSPVWKRAVIHLSGGKDFIIEAENLSEKNIYIQKVYLNGKPMDRLWLWHNEIING
                     GKLLFKMGDTPNKSLGTDPESLPPDFIK"
     gene            complement(3758..>4584)
                     /locus_tag="P5310_01280"
     CDS             complement(3758..>4584)
                     /locus_tag="P5310_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HRU32028.1"
                     /translation="DRIITVEDGETLSLGDKTLRFIYTPWVHWPETMSTYLVEDKILF
                     TCDFFGSHLATNELYARDKSRVYEAAKRYYAEIMMPFRSIIKKNLEKIKDIEISIIAP
                     SHGPLYDEPEFIMEAYEDWTSDKPKNTVVIPYISMHNSTRIMVEYLICSLTQKDIRVE
                     RFDLASVDLGKLAMSLVDAATVVVGTPTVLAGPHPNVVYAVYLLNALRPKTRYVSVMG
                     SYGWGGKTLEILKGMLTNLKVELLEPVLVKGLPREEDLKAIDNLANLIAEKHKEFI"
     assembly_gap    4585..4684
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4798..6129)
                     /gene="melA"
                     /locus_tag="P5310_01285"
     CDS             complement(4798..6129)
                     /gene="melA"
                     /locus_tag="P5310_01285"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HRU32029.1"
                     /translation="MSKKITFIGAGSLGFTRGLVRDLLTFPAFQDATLALMDIDEERL
                     NFARTAVEKIVKAGNYPAKIVATLDRTEALEGADGVVCTILAQNVNVWRSDIEIPKKY
                     GVDINVGDTRGPAGIFRALRTIPVMIDICKDIEKYAPNAVFLNYTNPMAMLCRAMQTA
                     SKVNVVGLCHSVQGTAEMLAKWIGAPMEEITYFCAGINHQAWFLDFKWNGKDAYPLIR
                     EAITTRPEIYNEEQVRNEMFLHLGYYVTESSGHNSEYNAWFRKRPDLIEKYCTHGTGW
                     NPGEYAYILKEYLRREDSWKDDVKYWLENAPIDLKRGKEYAAYIFNAVIGDGAMFEFN
                     GNIRNFGLIDNLPYGACVEVPIVASKRGFSPIKVGSLPTQVALLNYINSTCEELAVEG
                     SLTGDPMKVFHAICYDPLTSAVLSLAEIRDMVNEMFEASREWLPQFKHFKV"
     gene            complement(6361..>6573)
                     /locus_tag="P5310_01290"
     CDS             complement(6361..>6573)
                     /locus_tag="P5310_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32030.1"
                     /translation="DNINSIDIIQMSMVLYSLKKAQQIESAESLSLITRTLEKMEENL
                     QNTGEDIRNLEKEIYPWLGQNVDSLV"
BASE COUNT         1775 a         1332 c         1289 g         2077 t
ORIGIN      
        1 aacacttgta tatttacctt atgtactacc tgcagttgct gttggtttta tatggctctg
       61 gatgctaaat ccttattatg ggttggtaaa tctgttctta aatgcagtat ttcatatcca
      121 gggacctggg tggttctctg accctaagtg ggctaaacct gcatttgtaa tgttagccca
      181 gtggggagcc ggtggttctg cggtaatatt tttatctgcc attaaagatg ttccaagaga
      241 aatgctggag tctgctgaga tagatggcgc aggatggtgg acaaagttta gtaagattat
      301 tatccccatg ataagtccgg ttatactgta tcatataatt acagctctat ttggtggatt
      361 tcagatattt actactgcct atattgctac taatggtgct ccagctaatt ctaccctgtt
      421 ctatgtgttg tatctctaca ggaatgcatt tcagtacggt aagatgggat atgcgtctgc
      481 tatggcatgg atactcttta tcctggttct tattattact ttaataatat tcaggacctc
      541 tgcaagatgg gtcttctatc aaggaggaga atagccgtat gaatctaaga tggtttctct
      601 caaaagtggt aaccagactt gtattaattt tggtaagtgc tctatattta gttccgatat
      661 tctggatgtt taccacttct ataaaggata tgtctcaggt aatggcatat cctcctatct
      721 ggtggcctga tcctcctaag tggcataact atgtagaggc ggtaaattat ataccttttg
      781 ggcagtatac tctgaatacc atactcatat gcgccgggaa tatagtggga gttctttttt
      841 cctgtcctct tgtcgcctat agtatctcaa agataaagtg gagagggaga aacttccttt
      901 ttatgctgat tctcagcact atgctcatcc cttttcctgt aactatggtt ccactctttg
      961 ttgtctttgc cagactggga tggataaata catttacgcc attaatacta cctgctttct
     1021 ttggtggagg ggcattcaat atatttcttt taagacagtt cttcttaaca atccctgaag
     1081 aattatcgga tgcagccaga attgatgggg catctgaatt tggtatctat tcaagaataa
     1141 tacttccact tataaaacct atccttatag taattgctct gtttacattc ttaggagcct
     1201 ggaatgattt tatgggtcca cttatctatc tccaggacga agcaaagtat acacttgctc
     1261 tcgggttaca gatgtataga agtaccaact atgtggagtg gcctctactt atggcagctg
     1321 caacaatagt agttattcct acaattatcg ccttctattt tacccagaat accatagttg
     1381 aaagtgttac ccttacaggt cttaagggat aagtaatatg agtttcccgt gtaaaatact
     1441 tatctctctt cttatttttg cgtttctttt tcctctcccc tgtgctggag gagaggattt
     1501 atttgtaaaa tatgttgacc catttacagg aactggagga catgggcata cattcccagg
     1561 ggtaactgtg ccttttggta tggttcagct cagtccagac acaggtgtag aaggttggga
     1621 ctggtgttct ggctatcact attctgacag ctctataatg ggattcagcc atactcatct
     1681 cagtggaaca ggtgcggctg attatggaga ggtaagagta atgccagtgg taggagattt
     1741 aaaaataatt cctggaccta aagataaccc gaatataggt tacaggtcaa ggtttagcca
     1801 taagaaagag atagccagac ctggatatta ctctgtattt ctggatgatt acaaagtgca
     1861 ggctgaactg acagcatcac agcgggttgg atttcacaga tatacatttc caaagactga
     1921 taagggttat ataatctttg acctctacaa tagaatagga gatatggctg aaagcgcatg
     1981 ggttaaaatt gttggtaatg atgagataga aggttcgata accggtggac acttctgcgg
     2041 ggcgagagac cctcagacga tatactttgt ggctaaattc tcaaaacctt ttaaggagtt
     2101 tgggacatgg aaggtattca gggtggataa gggtaacagg gaagaaaaga tgctaaacag
     2161 gtcagagcct tttattgggg cttatgtcag atattctacg tctgaaaagg aaaagatact
     2221 gataaaagtt gccatctctt acacaggggt ggacggagcc agaaaaaatc tgcaggaaat
     2281 ccctgattgg aattttgaca gggtaagaga gaatgcggag aagttgtggg agaaagagct
     2341 tggtaagata gaagtagaag ggaatgaaag tgataagacc aagttttata ccgctctcta
     2401 tcattcattt attcatccaa gtttattctc agatatagat ggtaaatata tcggaacaga
     2461 tgacaaaatt tattcttcta cctatgacca ttataccgtc ttctctctat gggacacctt
     2521 cagatctctc catcccctgt ttctgcttat acagccacag aaaaatactg atatgataaa
     2581 gagtttcctg gatatatact ctcagtcagg ctggcttccc agatggcata aggggaacag
     2641 agagaccaac tgtatgatag gaactcacag tgactctgtt atagccgatg ccattattaa
     2701 aggtttacgc gattttgatt tagagaaagc ttacagtgct atgtataaaa atgcaatggt
     2761 agagtccatg gatttcaacc aggggcgaag aggaatcacg gagtataaaa aatcaggata
     2821 tgtccccggt gataagtttg accagtctgt gtcgagaaca ctggaatatg cctataatga
     2881 tttctgttta gctcaggtct caaaggttct tggtaaagaa gaggactaca aacttttcat
     2941 ctccagggct caaaattata aaaatctata tgattctaat gtcggattta tgagacccag
     3001 agatagtaat ggaaactgga taagctttaa gacatctttc tccagtagct ttgatccaac
     3061 agatgcaact tcccctcact ataccgaggg caatgcctgg cagtggacgt tttttgcccc
     3121 ccaggacccg tatggtttga ttactctttt gggaggagag gagaaattta tcaaaaagct
     3181 tgatgagttc ttcaatacta aatcacccat aaaaggacca ccagatatta ctggtcttat
     3241 tggacagtat gcacatggga atgagccatc tcatcatata tcttattttt atgtatatgc
     3301 caggatgccc tggaagactc aggagatggt aagaaagata gttgattctc tttatggaat
     3361 tggtccagaa ggactctgtg gaaatgaaga ttgtggacag atgtctgcct ggtatgtctt
     3421 tagctccctc ggattttatc cattttgccc gggaacgcct gtatatattt tgagttcccc
     3481 agtatggaag agggcagtga tacatttatc aggcgggaaa gattttataa ttgaagctga
     3541 aaacttatct gagaagaata tatacatcca gaaagtttat ttaaatggta aaccgatgga
     3601 tagattatgg ctatggcata atgaaataat aaacggtggg aaacttctgt ttaagatggg
     3661 ggatacaccg aataaatctt tagggacaga ccctgagagt ttaccgccag actttataaa
     3721 gtaataaaag gggcaccgta tagtgcccct tcagttttta aatgaattcc ttatgctttt
     3781 ctgctattag gtttgcaaga ttatctatag ccttcaggtc ttcttccctg ggtaatccct
     3841 taactaaaac tggttccaga agttccacct ttaaatttgt taacataccc tttagaattt
     3901 ccagggtctt tcctccccat ccgtatgaac ccatcacaga cacatatctc gtcttgggtc
     3961 tcagggcatt taatagatat accgcatata ccacatttgg atgaggacca gccagaactg
     4021 ttggcgttcc aaccaccact gttgccgcat ctacaagact catagccagt ttacccaggt
     4081 ccactgaagc caggtcgaat cgctcaaccc ttatatcttt ctgggtaaga gaacagataa
     4141 ggtactccac cataatcctg gtgctgttat gcatggatat gtaggggatt acaacggtat
     4201 tctttggctt gtcagatgtc cagtcttcgt atgcctccat tataaattca ggttcatcat
     4261 ataaaggacc atggctcggg gctattattg atatttctat gtcttttatc ttctccaggt
     4321 tctttttaat aatactcctg aatggcatca taatctcagc atagtatctc ttcgcagcct
     4381 catataccct ggacttatct ctggcataga gttcgtttgt tgccagatgt gacccaaaaa
     4441 agtcacaggt gaatagaatc ttatcctcca ccagatatgt gctcatggtt tctggccaat
     4501 gaacccaggg tgtgtatata aatctcagtg ttttatctcc cagagataat gtctctccat
     4561 cttctacagt gataatcctg tcctnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4681 nnnnccagta aacatctggc attatctgtc ttactttcat cagtgcctcc tagtaaacaa
     4741 ttattattaa attactatac attagtatat acttcgtctt tcaataagtc aatatctcta
     4801 taccttgaag tgtttgaact gtggtagcca ctccctactt gcttcgaaca tctcgtttac
     4861 catatctctt atctctgcca gactcagtac cgcagatgta agcggatcat agcatatcgc
     4921 atggaagacc ttcatcgggt caccggttaa cgaaccttca actgccagct cttcacaggt
     4981 agagtttata tagttcagaa gtgctacctg agtcggtaat gaccccacct ttataggact
     5041 gaatcctctt ttcgaggcaa ctatggggac ctcaacacag gctccgtatg gtaaattgtc
     5101 tatgagtcca aagttacgta tattcccgtt aaactcaaac atagctccat cccctattac
     5161 cgcattgaat atgtacgctg catactcctt acctcttttt aaatctatgg gagcattttc
     5221 gagccagtat ttcacatcat ctttccagct atcttctctt ctgagatatt ctttcaggat
     5281 ataggcatac tcccctggat tccatcctgt tccgtgtgta cagtattttt ctataaggtc
     5341 gggtcttttt ctaaaccagg cattgtattc agagttatgt ccacttgatt cggttacata
     5401 ataaccaaga tggaggaaca tctcattccg tacctgttct tcgttatata tttctggtct
     5461 ggttgtaatc gcctctctta ttagaggata ggcatctttt ccattccatt taaaatcaag
     5521 gaaccatgcc tgatgattta tccccgcaca gaaataagtt atctcttcca ttggtgcacc
     5581 aatccatttt gccagcatct ctgctgttcc ctggacactg tgacagagtc ctaccacatt
     5641 gaccttactt gcagtctgca tggctctgca tagcatagcc ataggatttg tgtaatttaa
     5701 aaatacagca ttgggtgcgt atttttctat gtccttacat atgtcaatca ttacagggat
     5761 agtccttaaa gctctgaaga ttcctgctgg accccttgta tccccaacat tgatatctac
     5821 cccgtatttc ttcggtatct caatgtcact tctccagaca tttacgtttt gggctaatat
     5881 ggtgcataca acgccatctg ccccctccaa tgcctccgtc ctgtccagtg tagccactat
     5941 ttttgctgga tagttcccgg ctttaactat cttttcaact gcagtcctgg caaaattcag
     6001 cctctcctca tctatatcca taagtgctag ggtggcatcc tgaaatgctg gaaatgtgag
     6061 taaatccctc accaaacccc tagtaaatcc cagacttccc gcaccgataa atgttatctt
     6121 tttagacata aactcactcc cttctgatgt ttttatcttt tgaattatat cataagatga
     6181 cagaatttaa ttcttcttca aataatatct ttaatgcaac aagtctcttc ctggcttttt
     6241 gtatatggct ttctgcaagt cttatgtttg aaatatctct acaccttcct ctttgcctac
     6301 attaatatcc ttttgccagc ctgaacacag tctggataag cttgtcaaac tcttctctaa
     6361 tcataccagg ctgtctacat tctgtccgag ccagggataa atttctttct ccagattgcg
     6421 aatgtcctct cctgtatttt ggagattctc ctccatcttt tcaagtgttc tggttattaa
     6481 gctgaggctt tcagcagatt ctatctgctg ggctttcttg agagaataca gcaccattga
     6541 catttgaata atatcaatac tatttatatt atc
//
LOCUS       DAOTKU010000028         6571 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_11053_length_6571_cov_3.505064, whole genome shotgun sequence.
ACCESSION   DAOTKU010000028 DAOTKU010000000
VERSION     DAOTKU010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6571
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11053_length_6571_cov_3.505064"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /locus_tag="P5310_01295"
     CDS             complement(<1..557)
                     /locus_tag="P5310_01295"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HRU32031.1"
                     /translation="MSMIRINKDLCKGCVNCIRVCPTEALRVRFGKVVLLEDRCIDCG
                     ECIIACPEHAIEAETEEVRDMSNYRMKVALVSSVLYAQFGLDVSPYLIRSAVKEMGFD
                     LIMDVAFGTEVSTLYLRELVRENPEKLYISSMCPSVVKLIQIRFPSLVECLTPIASPE
                     EISARYIKEKLISEGYREDEVGVFL"
     gene            complement(568..1434)
                     /locus_tag="P5310_01300"
     CDS             complement(568..1434)
                     /locus_tag="P5310_01300"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRU32032.1"
                     /translation="MREIERDFYALLTLPVSLIMNSNMPKILPDRKVSQVREILRINQ
                     LDGVAITDEESRLVGIVTMYDLLKAIEENKRDVPVEEIMTEDVITLSPHNTLADAIEY
                     FRVYGYGRFPVVDENKVFLGMVTPLDIVIRLPSLKEEKEEIKEEEKSRVVRLEFSIEG
                     GDFINGGEASSKIKRYLQNLGLPNHIIRRVAVASYEMEMNMVIHAYRGKLLIEISSDK
                     IVIKAIDEGPGIEDVEKAKQPGYSTASPKVRELGFGAGMGLPNIISCSDEFDIKSAVG
                     VGTEVTSIIYIR"
     gene            complement(1431..1766)
                     /locus_tag="P5310_01305"
     CDS             complement(1431..1766)
                     /locus_tag="P5310_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32033.1"
                     /translation="MKLKDLEELLSAKVLYGESLLDKDVDYFYASDLMSDILATVKEP
                     GVLITAMVQPQVMRTIQMLDLIGVIFVNGKCPPEDTLRLARSIEIPVLVTRCSMFETC
                     GKIYMSLVK"
     gene            complement(1787..3088)
                     /locus_tag="P5310_01310"
     CDS             complement(1787..3088)
                     /locus_tag="P5310_01310"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HRU32034.1"
                     /translation="MDISYQDAIEIIEGLSNFGSKLGLDRIKRLLDRLDNPQDKIPVI
                     HIGGTNGKGSVSRMISSVLTRAGYKVGLYTSPHLHSIRERIQINNRLIDKDSFVNTLK
                     ILLPYIEELTDEDADFYLTEFEILTAMAFLYFYMSKVDVVVLEVGLGGRLDATNVVNN
                     VLVSVITNVDWDHMDRLGNTLSLIGREKAGIIKRGVPVVTGARGEGLDVIADTAKKLD
                     SNLYILGRDFSADWYVLSQAGTEMGFRNEIKREELFTGLRGTYQVDNMSIAMMGLEII
                     KNSLHFSDSSLFQGLAETFWPGRMEIVSRDPLVLLDGAHNKGGAEKFASSLKILFDIK
                     PTLVVGMLKDKDSYGIISSVLSLAKEDVVVTSPRNSRALDAEALGKVVKDLGGNPIVV
                     PDVKESVKMAIQLDSDFISVMGSLYVVAEAREVFVNGIERD"
     gene            complement(3092..5704)
                     /locus_tag="P5310_01315"
     CDS             complement(3092..5704)
                     /locus_tag="P5310_01315"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012447567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HRU32035.1"
                     /translation="MRELAKVYNPGEIAQKWIDIWFSSDIFAPDFNRGSEAFSIVLPP
                     PNVTGNLHIGHALGFTLDDVVVRYKRMKGFVTLWVPGTDHAGIATQNVVEKELAKEGL
                     SRYDLGREKFLEKIWEWKDRYRNNIVNQLKQMGFSCDWSRERFTMDEHCSRAVRTAFV
                     SLYKKGLIYKGERIINWCPRCKTALSDIEVEYDETKDKLYYIKYPLKDGGYITIATVR
                     PETMLGDTAVAVNPQDDRYRGLIGKIAILPLVGRELPIIADEAVESDFGTGALKVTPA
                     HDPADFEIGIRHRLDSIRVIGLDGRIDCPDMDTYTGLDRFEARKLIVRHLEENGYIEK
                     IEDYTHSVGQCERCGTIVEPLLSSQWFVSMKPLAQKALERDNEVRFIPERWRKVYHDW
                     LLNIRDWCISRQIWWGHRIPVWYCGDCNETIVEMEDPTSCPKCGGNNLKQEEDVLDTW
                     FSSALWPMSTLGWPDETEDFKKFYPTSLLITGYDIIYFWVARMIMMGLEFSDKMPFKD
                     VYITGLVRDEKGRKMSKSLGNVIDPMEIVRDLGTDSLRFALTSLVTEGQDVNIGNEKL
                     EGARNFANKIWNAGRFILMSISDPEVPSSLNELDIFSRWILTVMEETIEKATICYDKY
                     DFNEITQLLYSFFWGDFCDWYIEVSKILLKTNPGHTSWVLLTVFRNYLKLLHPIMPFI
                     TEELWSYVKDEGDDILSLSNWPADTGFKDQDSVKKVNLIFDIIKAMRSLKQEMGIPFE
                     KKIAGFVHSRMYNELLLSNVRVISTLARIEPIDILPELLPPRKGIISNIQPDFEVYLE
                     VGGVINIDEEIERLKKKMEEIDLQICKIESRLSNRDFLLKAREEAKEKERNRRENLLK
                     EKEKLLQRLALLSS"
     gene            complement(5688..>6571)
                     /locus_tag="P5310_01320"
     CDS             complement(5688..>6571)
                     /locus_tag="P5310_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HRU32036.1"
                     /translation="ILDLNGYTEEEKLNIASRFLIPRLSKEHNINRKNIPMKEDIILK
                     IIREYTREAGVRNLERELAKIFRKVVVSYLEGKKGVFKVDEESLIELLGPPRFLSESI
                     EKKPEVGLANGLAWTEAGGEVLPIEVIYMKGKGNLILTGQLGDVMRESAQAAFSFIKN
                     RSVYLDITHNFSEIDVHIHIPKGAIPKDGPSAGITMASALASSLSGIPLRQDVAMTGE
                     ITVRGRVLPVGGIKEKLLAAYSAGIREVIIPRENESDLSKIPDEIKKNLIIRKVSHMD
                     EVFPIIFYKEDVLERVS"
BASE COUNT         1884 a         1627 c          965 g         2095 t
ORIGIN      
        1 ataaggaata ctccaacctc atcttccctg taaccttcag atataagctt ttcttttata
       61 tatctggctg atatctcttc aggtgaggct ataggggtaa gacactctac cagagaaggg
      121 aatctgatct gtatcaattt aacaactgaa ggacacattg aagatatata aagtttttca
      181 ggattttctc ttactaactc tctgagatag agggtagaaa cctcagtgcc aaaagccaca
      241 tccataataa ggtcaaaacc catctctttt acagcggacc ttataaggta tggtgataca
      301 tccaatccaa actgggcata aaggacagaa cttacaagag caaccttcat cctatagtta
      361 gacatatccc tgacctcttc tgtctctgct tctatggcat gttcaggaca ggctattata
      421 cactcaccgc aatctataca tctatcctct aaaagaacca ccttaccaaa tcttaccctc
      481 aaagcttccg tgggacaaac ccttatacag tttacacacc ccttacacag gtccttgttt
      541 atccttatca tactcataac attcatccta ccttatatat attattgatg ttacttctgt
      601 gcccacccca acagcactct ttatatcaaa ctcatctgaa caggagatta tatttggaag
      661 tcccatcccg gcaccaaatc ctaactctct cacttttgga gaggcggtcg aatagccagg
      721 ctgtttggct ttctccacgt cttctatacc aggtccctca tctatagcct ttataacaat
      781 cttgtcagaa gatatctcaa taagtaattt acctctatag gcatgtatta ccatattcat
      841 ctccatttca taactggcga cagctactct tctaataata tgattgggta atcccaaatt
      901 ttggaggtat ctttttatct tacttgaagc ctctccacca ttaataaaat ctccaccctc
      961 aattgagaat tcaagcctta ctaccctgct cttttcctcc tctttaatct cttccttctc
     1021 ttctttcaaa ctgggaagtc ttataactat gtctaaaggg gttaccatac caaggaaaac
     1081 cttattctca tcgacaacag gaaatcttcc atacccatag acacgaaaat actctatggc
     1141 gtccgctaaa gtattatgag gagaaagagt tataacatcc tcagtcatta tctcttcgac
     1201 aggaacgtcc ctcttatttt cctctatagc cttgagaagg tcatacatgg tgactatacc
     1261 caccagccta ctctcctcat cggttatagc gactccatcc agttgattta tccgcagtat
     1321 ctctcttacc tgtgagacct ttctatcagg caagatcttt ggcatattag aattcattat
     1381 aagtgagact ggaagtgtta aaagggcata aaaatctctt tcgatttctc tcattttacc
     1441 agagacatat atatctttcc acaggtctca aacatagaac atctggtaac cagtactgga
     1501 atttcaatag atctggctag cctgagcgta tcttctggcg gacacttacc atttacaaag
     1561 ataactccaa taaggtccag catctgaata gtcctcatta cctgaggttg aaccatagcg
     1621 gtgatcaaaa cacctggttc cttaactgtg gctaatatat cactcataag gtctgaggca
     1681 tagaaataat ccacatcctt atcgagcagg gactctccat ataatacttt agcagacagt
     1741 aattcttcaa ggtcttttaa tttcattatt gattcacctc atgtatttag tctctctcta
     1801 tcccatttac aaatacctct cgggcttctg caactacata tagagagccc ataacagaga
     1861 taaagtcaga gtccaactga atcgccatct taacactttc tttcacatca ggaacaacta
     1921 taggattacc tcccagatcc ttaaccactt tacccaaagc ttctgcatcg agagctctgc
     1981 tattcctagg tgacgttaca acaacatctt cttttgccag agatagtact gaacttatta
     2041 taccatagga atctttatct ttaagcattc ctacaaccag tgtgggttta atatcgaata
     2101 gaatcttcag agaactggca aatttttcag ctcctccctt attatgagca ccatcaagta
     2161 atactaacgg gtctcttgaa acaatctcca tcctccctgg ccagaatgtc tcagccaaac
     2221 cctgaaaaag actagaatct gaaaaatgta aactattttt tattatctct aatcccatca
     2281 tagcgatgga catattgtct acctgatatg tacctctgag cccggtaaaa agctcttctc
     2341 tctttatctc atttctaaaa cccatctcag taccagcctg agatagcaca taccaatctg
     2401 ctgagaaatc tcttcccaag atatatagat ttgaatccag cttcttagca gtatcagcta
     2461 ttacatctaa cccttctccc ctggctcctg taacaaccgg gactcctctc tttattatcc
     2521 cagccttctc tctacctatg agacttaaag tattacccaa tctatccata tggtcccaat
     2581 ccacattggt aatcactgag accaaaacat tatttaccac attggtagca tctaatctcc
     2641 cacccagtcc aacttccaaa actactacgt ctaccttaga catataaaag tacaggaacg
     2701 ccatagcggt cagtatctca aactcggtaa gataaaaatc ggcatcttca tcagtgagct
     2761 cttctatata ggggagcaga atctttaatg tatttacaaa agaatctttg tctattaatc
     2821 tattattaat ctgtatcctt tcccgtatcg aatgaaggtg aggagatgta taaagtccaa
     2881 ccttatagcc agctctggta agtacagatg agatcatcct tgagacagaa ccttttccgt
     2941 ttgttccacc aatatgaata acgggtatct tatcctgagg attatctaat ctatccagaa
     3001 gcctcttgat acggtccaaa cccagctttg agccaaaatt agagagaccc tctataatct
     3061 ctatggcatc ctgatacgat atatccatct attaggaaga tagaagagct aacctctgta
     3121 agagtttttc tttctctttt aataaattct ctcttctatt cctctctttc tcttttgctt
     3181 cttccctggc ttttaaaaga aaatccctgt tgctaagtct tgattcaatc ttacatatct
     3241 gcaaatctat ctcttccatt ttcttcttaa gtctctctat ctcttcatct atatttatta
     3301 ccccgccaac ctcaagatat acttcaaagt ctggctgtat attagagata atccctttcc
     3361 gagggggtaa taactctggt agtatatcta tcggttctat cctggctaag gtagaaataa
     3421 cacgaacatt ggataaaagg agctcattat acatcctgct atgcacaaaa ccagctatct
     3481 tcttttcaaa cggtatcccc atctcctgct ttaaagacct catagctttt atgatgtcaa
     3541 atataagatt aactttctta acgctatcct ggtctttaaa gcctgtgtca gctggccagt
     3601 tagagaggga taaaatatca tctccttcat cctttacata actccagagt tcctctgtaa
     3661 taaatggcat aatgggatgg aggagtttaa gataatttct aaatacagta agtagcaccc
     3721 aacttgtatg acccggatta gtcttcagga gtatctttga tacctctata taccaatcac
     3781 aaaaatcacc ccagaaaaag ctgtacaaaa gctgagttat ctcattaaaa tcatacttat
     3841 cataacatat agtggctttc tctatcgtct cttccataac tgtcaatatc cacctactga
     3901 atatatccag ttcattaagc gaagatggca cttctggatc agagatactc ataagaataa
     3961 atcttcctgc attccatatc ttattggcaa aatttctagc accttccagt ttttcattcc
     4021 ctatgtttac atcctgtcct tctgtgacca gagatgttaa ggcaaatctc aaactatctg
     4081 ttccaaggtc tcgaactatc tccatagggt ctatcacatt ccctaaagat ttactcatct
     4141 tacgcccctt ctcatctcta accagccctg ttatatagac atctttaaat ggcatcttat
     4201 cactgaactc caaacccatc attatcatcc ttgctaccca gaaataaata atgtcataac
     4261 cggttatgag aagagaagtc gggtaaaatt tcttaaagtc ctctgtttca tccggccagc
     4321 caagggtact cataggccag agggcagaac tgaaccaggt atctagaacg tcctcttcct
     4381 gttttaaatt atttccccca cacttagggc aggaagtcgg atcttccatt tcaactatgg
     4441 tttcattaca atccccacag taccataccg gtatcctgtg tccccaccat atctgtctgg
     4501 aaatacacca gtctcttata tttaacaacc aatcatgata tacctttctc catctctctg
     4561 gtataaatct tacctcatta tcacgttcta atgccttttg agccaggggt ttcattgaaa
     4621 caaaccattg ggatgagagt aagggttcaa ctatagttcc acatctctca cattgcccta
     4681 cactgtgggt ataatcttct atcttttcta tatatccatt ctcttccaga tgccttacta
     4741 tgagttttct ggcttcaaac ctgtccaaac cggtataggt atccatatct ggacaatcaa
     4801 tcctgccatc caaacctata actctgatac tatcaagcct gtgtctgata cctatttcaa
     4861 agtctgccgg gtcatgggca ggggtaacct ttaaagcccc cgttccaaaa tctgattcta
     4921 cagcttcatc agctattatc ggtaactctc taccaaccag tggcagtata gctatcttac
     4981 caataagtcc tctatatcta tcatcctgag gattcactgc tacagcggta tctcccagca
     5041 tggtctcagg tcttaccgta gctatagtaa tatatcctcc atcctttaat ggatatttta
     5101 tatagtatag tttatcttta gtctcgtcat actctacctc tatatcagag agagctgtct
     5161 tgcatctggg gcaccagttt attattcttt cccctttata gataagtcct tttttatata
     5221 gagagacaaa tgctgttcta accgctctag agcagtgttc atccatagta aatctctctc
     5281 tagaccaatc acagctaaag cccatctgtt tgagctggtt aactatatta tttctgtatc
     5341 tatctttcca ttcccatatc ttttccaaaa acttttctct tccaaggtca tatctactca
     5401 acccttcttt agccagttct ttttccacta cattctgagt agctatccct gcatggtctg
     5461 tacctggaac ccatagagtt acaaaaccct tcatcctctt atatctaacc accacatcgt
     5521 ccagggtaaa accaagtgca tgccctatgt gaagattacc agttacattt ggaggtggta
     5581 atactatgga aaatgcctca ctacccctat taaaatcagg ggcaaaaata tctgagctaa
     5641 accatatatc tatccatttt tgagctatct ctcctggatt atagacctta gctaactctc
     5701 tcaaggacat cctccttata gaagattatt ggaaagactt catccatatg agaaaccttt
     5761 ctaatgatca agttcttctt tatctcatca gggatcttgc tcaggtcact ctcattttct
     5821 ctcggtataa ttacctctct gatgccagca ctataagctg ccagaagttt ttcttttata
     5881 ccaccaacag gaagaaccct tcctcttacc gtaatctctc cagtcatagc cacatcctgt
     5941 cttaatggta tacctgaaag gcttgatgct aaagcggatg ccatagtaat ccctgctgag
     6001 ggaccatctt ttggtatagc ccccttgggt atatgtatat gaacatctat ttcactgaag
     6061 ttatgagtaa tatccagata gacgcttcta ttctttataa agctaaaagc tgcctgagca
     6121 gattctctca taacatcacc aagctgaccg gtaaggataa ggttaccctt tcccttcata
     6181 tatataactt ctatgggtag gacttctccg ccagcttctg tccaagctaa accatttgcc
     6241 agtcctacct ctggtttctt ctctatagat tcactcaaaa atctgggagg acctaacaat
     6301 tctatcaggc tctcctcatc aaccttgaag actcccttct taccctccag atatgatacc
     6361 acaaccttac ggaatatctt tgctaactct ctttcaagat ttcttactcc agcctctcgg
     6421 gtatactctc ttataatctt caatataata tcttccttca tggggatatt ctttctatta
     6481 atattgtgct ccttagacaa tctcggtata agaaagcgac tagcaatatt gagcttctcc
     6541 tcttctgtat agccattcag atccagtatc t
//
LOCUS       DAOTKU010000029         6443 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_11269_length_6443_cov_3.151691, whole genome shotgun sequence.
ACCESSION   DAOTKU010000029 DAOTKU010000000
VERSION     DAOTKU010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6443
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11269_length_6443_cov_3.151691"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            13..888
                     /gene="murQ"
                     /locus_tag="P5310_01325"
     CDS             13..888
                     /gene="murQ"
                     /locus_tag="P5310_01325"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007131761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HRU32037.1"
                     /translation="MTENIDMMSTLEIVRVMNEEDKKVPLAVEKVLPDIARAIDLIVE
                     RLKQRGRLIYVGAGTSGRLGVLDASECPPTFGVNPEMVQAIIAGGDKAIRSAVEYAED
                     DIEQGREEIKNRGVSSKDAVTGITASGTTPFVLSALEEAHSLGAVTVLITNNYNSPAK
                     AFVDVAIEIDTGPEVIAGSTRLKAGTSQKLVLNMLSTASMIKLGKVYKNIMVDVRPTN
                     EKLVNRAVRIITDITGVSDDIALERLKMCGMNVKEAIVGIVGDLSPQESKRILEESGG
                     YLRLALERVKGNNPG"
     gene            901..1944
                     /locus_tag="P5310_01330"
     CDS             901..1944
                     /locus_tag="P5310_01330"
                     /inference="COORDINATES: protein motif:HMM:NF016145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HRU32038.1"
                     /translation="MDRQILLVGYFGFDNLGDELLLISLLDFIRHNFQDIKPLVLYGK
                     NLPSVYGADVVPRKKLLSGIIKADGVCFSGGSIIQDATSVRSLVYYLGIILLSFITGK
                     PVIMVSQGFGPVETWLGKKLIRILDLVYSISVRDVDSYKFLERSGIRRPKIYQGNDLV
                     SFLDIDRFRIEDSSVERDVLVSVREAEGFREEEFLDAFVRFREENNLNLAFFVTHKDE
                     DARITEEFARKLDCEILFWDDPISAMKILSSARFIVSMRLHPLIISSLLEIPFLGIIY
                     DPKVRSFVSLFPDAHSLDISSDSSQIEDTLNLAWRNRERLKSGIIKYRDNLNKQETFN
                     PIYDLYSLWSKDR"
     gene            1910..2629
                     /locus_tag="P5310_01335"
     CDS             1910..2629
                     /locus_tag="P5310_01335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043163 - cell envelope organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HRU32039.1"
                     /translation="MTCTVFGLKIDNVDRREAVQVSEGFLSEDRFHYIVTLNPEGVVI
                     AQENTGFISAVNQADLVVADGSWLVRAIRFLGEDLKERVAGIDLLLDILSLCRDKSFS
                     VYLLGSREDIVSDAKKNLEDRFPGINIAGFHSGYFDREEEIKIIEEIERLKPHLLVVG
                     MGMPKQELWISRHRDLPVRLAIGVGGSFDVLSGIIPRAPHWMQASGLEWLYRILKDRI
                     RRLSFIPKFSLLVLKSKLNIV"
     gene            2637..3716
                     /locus_tag="P5310_01340"
     CDS             2637..3716
                     /locus_tag="P5310_01340"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HRU32040.1"
                     /translation="MKILDKYLLREVASPFIVGVLGFSLILSASLLFNLADLFVNRGV
                     PLINIIKILFYEFPGVLTISFPISALFATLIGLNRLVREGEITALRIGGIRLTRIFLP
                     IIIFGIVVSLGNFGISEFVAPWSVHQGETLIREAIFEDPLPVIENNVFFKAPDNRYVY
                     LGRYDPSTRTGNDILLYSIPSNSPLQVISAKRVIVENKVWILEEGAIHYYDEDGIISL
                     QSKFERLSLNLDVDAETFYKGQKTTSEMSARELKERIDSFKKSGIDTRNLEVDYHLKF
                     SIVFAAAILSLVGSPLSILPVRGGRFYGLSASLIIVFIYYTILALGRAFARNGLFSPM
                     FGVWLPNIVGLVLGIFLMFYKERRG"
     gene            3721..4362
                     /locus_tag="P5310_01345"
     CDS             3721..4362
                     /locus_tag="P5310_01345"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HRU32041.1"
                     /translation="MFKDRVDAGIKLGLALKELSIQDPVVLGIPRGGVIVAVEVAKIL
                     KAPLDIAIARKIGAPFDREFAIGAVNSLGNLILNENSIVEMGISSEYVKTAVKEELEE
                     IKRRLSIYRGERDTVDLKDRSVIIIDDGLATGLTMLSVVKGVKSSNPKETIVAVPVAS
                     SGAVERLETETKVFALEIPEYFYAVGQFYEDFRQVSDAEVISALNSVWSEMQQ"
     gene            4341..5519
                     /locus_tag="P5310_01350"
     CDS             4341..5519
                     /locus_tag="P5310_01350"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009051779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HRU32042.1"
                     /translation="MVGDAAMRLFIVVMDGVGIGELPDAHRYGDEGSNTLGNTVRVVG
                     KMEIPNLMSLGLGELVPELPGESRIIGAYGKMVEKSVGKDTLIGHWEMMGIITVEDFP
                     TYPDGFPSEVISRFEAEIGRKVLGNKPASGTEIIKELGELHMKTGYPIVYTSADSVFQ
                     IAAHEEIIPVEELYNYCRIARRILAGKHGVARVIARPFLGTPGNFYRTERRKDFSLPP
                     PEKTALDLLVENGITTIGLGKIEDIFSNQGLAESNHTHNNEETMSALIEIEKNREDRL
                     MVFANLSDFDTIYAHRNNPRGLAEALERFDVSLGEAMSILNDEDLLIVCADHGNDPTT
                     PSTDHSREYTPLLVKGRKDTPVYLGVRETFADVGATALEFFGIGEHKFPGKSFLDKLR
                     "
     gene            5516..6223
                     /locus_tag="P5310_01355"
     CDS             5516..6223
                     /locus_tag="P5310_01355"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HRU32043.1"
                     /translation="MILTGCCGWGYLRIPEDLKKDYRSVLSYYSSLFDVVEVNSTFYH
                     IPNISIAEKWRQEVRSDFVFTVKVSRVITHLDRFSTDKSRDLISFYIDFAEKLDATIL
                     LFQLPSTFPEKNMNNMSAVLKSFHDTTVRFAVEARNLSLEKRNEFAMETDTIPVIDPF
                     TAIDSSFRPRVIHNTSYFRLHGSPPGKVMYGYDYTDCDLERLLSYIQPVKDSNVYVLF
                     NNTEMYKNALRFKEMVS"
     gene            6224..>6443
                     /locus_tag="P5310_01360"
     CDS             6224..>6443
                     /locus_tag="P5310_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014451638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRU32044.1"
                     /translation="MVEAMFYEKLKDSLVRCTLCPRYCVIKPEERGYCGTRLNKDGIL
                     YAVSYGVVSSIANDPVEKKPLFHFYPGSM"
BASE COUNT         1884 a         1051 c         1646 g         1862 t
ORIGIN      
        1 gattataaca ggttgacaga gaatatcgat atgatgtcaa cccttgagat agtcagggtt
       61 atgaatgaag aggataaaaa ggttcctctg gctgtggaga aagttctacc tgatatagca
      121 agggctatag accttatagt ggaaaggctg aaacagaggg gaaggcttat ctatgttggg
      181 gcagggacaa gtggaaggtt aggagtactg gatgcttcag agtgtccacc tacatttgga
      241 gtaaatccag agatggtaca ggctataata gcaggaggag ataaggctat tcgttctgct
      301 gtggagtatg ctgaagacga tatcgagcag ggaagggaag agataaagaa tagaggagtg
      361 tcttcaaaag atgcagttac tggtataact gcaagtggaa caaccccttt tgtcttatcg
      421 gctctggagg aagcccacag tcttggggct gtaactgttc tgataacaaa taactataac
      481 tctccagcta aagcattcgt tgatgtagca atagagatag atacaggacc agaagtgata
      541 gcaggctcga ctaggctcaa ggcaggtaca tctcagaaac tggtattgaa tatgctcagc
      601 actgcaagca tgataaaact tggtaaggtt tataagaata ttatggttga tgtaagacct
      661 accaatgaga agcttgtaaa cagggcggtc aggataataa cagatatcac cggggtctca
      721 gatgatatcg ctctggagcg attgaagatg tgtggtatga atgtaaagga agcgatagtg
      781 ggcatagtgg gagatttaag ccctcaggag agtaagagga ttttagaaga atcaggaggt
      841 tatctcaggc tggctctgga gagagttaaa gggaataatc cgggataggt ctctgttaaa
      901 atggacaggc agattctgct agtaggttat tttggttttg ataatctggg tgatgaactt
      961 cttcttatct ctctcctgga ttttatcaga cataatttcc aggatataaa gccactggtt
     1021 ttatatggta agaatctacc cagtgtatat ggagccgatg ttgttcccag aaagaagctc
     1081 ttatctggaa taataaaggc agacggtgta tgtttttctg gaggcagtat cattcaggac
     1141 gctacaagtg taaggagtct ggtctattat cttggcataa tattgctgtc tttcatcaca
     1201 ggtaagcctg taataatggt atctcagggt tttggtcctg tagaaacctg gctcgggaaa
     1261 aaacttatca ggattcttga tctggtgtat tcaataagcg tgagagatgt ggattcatat
     1321 aaatttcttg agaggtctgg tatcagaaga cctaaaatct atcaagggaa tgacctggta
     1381 tcatttttag atatagatag attcaggatt gaggattctt cagtggagag agatgttctt
     1441 gtgtctgtta gagaagctga gggtttcagg gaagaggagt ttctggatgc gtttgtcaga
     1501 ttcagagagg agaataatct gaatctggct ttcttcgtaa ctcacaaaga tgaggacgct
     1561 cgaattacag aggaatttgc caggaaactt gattgtgaga tacttttctg ggatgacccg
     1621 atatctgcta tgaagatact atcttctgca agatttattg tcagtatgag gcttcatcca
     1681 ttgataatat catcactgct agaaatcccg tttttgggaa taatctatga ccccaaggtg
     1741 aggtcatttg tatctctctt tccagatgcc catagccttg atatctccag tgattcttct
     1801 cagatagaag atactctgaa tctggcatgg agaaacaggg agaggctaaa atctggtata
     1861 attaagtaca gagataattt aaataaacag gagacgttta atcccatata tgacctgtac
     1921 agtctttggt ctaaagatag ataatgttga tagaagagaa gccgttcagg tatcagaggg
     1981 gtttctctca gaggatagat ttcattatat cgttacttta aatccagaag gtgttgtcat
     2041 cgctcaggag aatactggtt ttatttctgc agttaatcaa gctgacctgg tagttgcaga
     2101 tggtagctgg ttggtgaggg cgatcagatt tcttggtgaa gacttgaagg agagggttgc
     2161 ggggatagac cttctccttg atatactcag cctctgcaga gataaaagct tctcagtata
     2221 ccttttgggc tccagggaag atatcgtctc agatgcaaag aagaatcttg aagataggtt
     2281 tccaggtata aacatcgccg gttttcacag cggatatttt gacagggaag aagagataaa
     2341 aattatagaa gagatagaga gattaaaacc ccatcttctg gttgtgggaa tgggtatgcc
     2401 aaaacaggaa ctctggatca gcagacatag agatctaccg gtaagattag ccataggcgt
     2461 aggtggtagt tttgacgttt tgagcggtat aataccaaga gcccctcact ggatgcaggc
     2521 atcgggtctg gagtggttat acagaatttt aaaagaccgg ataaggagac tttcttttat
     2581 cccgaaattc tcattacttg tgctaaaatc taaactaaat atagtataat taaactatga
     2641 agatactgga taaatattta ctcagagagg tagcatctcc atttattgta ggcgtactgg
     2701 ggttttcttt gatactgtct gccagtcttc tctttaacct ggcggacctg tttgtaaaca
     2761 ggggggttcc attgatcaac ataataaaga tattatttta cgagttcccg ggggttttaa
     2821 ctataagttt ccccatatca gcactgtttg ccactttaat tggactgaac agactggtca
     2881 gagagggaga aatcactgca ctgaggattg ggggtataag acttaccaga atttttctac
     2941 cgataataat attcggaata gttgtaagtc tgggtaattt tgggataagc gagtttgttg
     3001 ccccctggtc ggttcatcag ggtgaaacat taataagaga ggcaatcttt gaagaccctc
     3061 ttcctgttat tgagaacaat gttttcttca aggctccaga taatagatat gtttatttgg
     3121 gtagatacga cccgagtaca aggactggaa acgatatcct attatattct atcccgtcta
     3181 actctcctct ccaggttatc tctgccaaga gggttatagt ggagaataag gtctggattt
     3241 tagaagaagg agccatccat tattatgatg aggatggcat tataagcctc cagagtaagt
     3301 tcgagagact ttctttgaat ctggatgttg atgcggagac attttataaa gggcagaaga
     3361 ctaccagcga gatgagcgcc agagaattga aggagcgaat agactctttt aaaaaatcag
     3421 gtatagatac gcgtaatctg gaggtggact atcatctgaa attctccatt gtctttgctg
     3481 cagcgatact ttctctagtt ggatctccac tcagcatact tcctgtaaga gggggaagat
     3541 tttacggtct cagtgcaagc ctgattatag tatttatcta ttatacaatc cttgcactgg
     3601 gcagggcgtt cgccagaaat gggttatttt ctccaatgtt tggtgtatgg cttcccaata
     3661 ttgttggtct tgtgcttggt atctttctta tgttttacaa ggagaggaga gggtagatta
     3721 atgttcaagg atagagtaga tgctggtatt aaactggggc tggcattgaa agaattatct
     3781 atacaggacc cggtggttct gggaataccc agaggcgggg tgatagtagc ggttgaggtg
     3841 gcaaagatct tgaaagcccc tttagatata gccatagcaa ggaagatagg tgccccattt
     3901 gacagggagt ttgctattgg ggctgtaaat tctctgggta accttatact caatgagaat
     3961 agtatagttg agatgggtat atccagtgag tatgtaaaga cagcggtaaa ggaagagctt
     4021 gaagagatca agagaaggct ctccatatat agaggggaga gagatacagt agacctgaag
     4081 gacaggtctg ttatcattat agatgatgga ctcgccaccg gacttactat gttatcggtg
     4141 gtaaaaggag tgaagtccag taatccgaaa gaaaccatag ttgcagtccc ggttgcttct
     4201 tcaggtgcgg ttgagagatt ggagacagag acaaaggtat ttgccttaga gataccagaa
     4261 tatttttatg ctgttggtca gttttatgag gattttcgcc aggtcagtga tgctgaagtt
     4321 atatcggcat taaattctgt atggtcggag atgcagcaat gaggctgttt atcgtagtta
     4381 tggatggggt tggaatagga gaactccctg atgcccacag gtatggggac gaggggagca
     4441 atactctggg taatactgta agagtggttg ggaagatgga gatacccaat cttatgagtc
     4501 tgggattagg agagctagtg ccagaactgc caggggagtc taggattatt ggagcctatg
     4561 gaaagatggt ggagaagtct gtagggaagg acacacttat cgggcactgg gagatgatgg
     4621 ggattataac ggttgaggat tttccaacct atcctgatgg ctttccatcc gaagttatct
     4681 ctaggtttga agcggagata gggaggaagg tccttgggaa taagcctgct tccggtactg
     4741 agattataaa ggagcttgga gagctccata tgaagaccgg ttatcccata gtctatacat
     4801 ccgctgatag cgtctttcag atagctgccc atgaggagat tattccagta gaagaacttt
     4861 acaactattg caggatagcc aggaggatat tagccggtaa acacggggtt gccagggtta
     4921 tagccagacc attcctggga actccgggca acttctacag gacagagaga aggaaggatt
     4981 tctctctacc tcctccagag aaaacagctc tcgatctgct ggttgaaaac ggtattacca
     5041 cgataggtct tggtaagata gaggatatat tcagtaatca ggggctggca gaatctaacc
     5101 atacacataa taatgaggag acaatgtctg ctcttatcga gatagagaaa aatagagagg
     5161 atagattgat ggtatttgct aacctctcag attttgatac tatttatgcc cacagaaata
     5221 atcccagagg tcttgccgag gctcttgaga ggtttgatgt ctctttgggg gaggctatga
     5281 gcatattaaa tgatgaagat ctccttattg tctgtgcaga ccatggaaat gaccccacaa
     5341 ctccaagcac tgaccattca agggagtata cacccttact ggttaagggg agaaaggata
     5401 ctccggtcta tcttggagtg agggaaacat ttgcagatgt gggagctact gctctggaat
     5461 tttttgggat aggtgagcat aaattcccgg ggaagagttt tctggataaa ctgagatgat
     5521 tcttactgga tgttgtggat ggggttattt gaggatacct gaggatttga agaaagatta
     5581 caggtcagtt ctttcctact atagttctct gtttgatgtg gtggaggtaa attctacatt
     5641 ctatcatatt ccgaatatat ctatagcaga gaagtggagg caggaggtca ggtctgattt
     5701 tgtattcacc gttaaagtct ccagggttat cacacatctg gataggtttt ccactgataa
     5761 gtctagagat ctgatatctt tttatattga ttttgcggaa aaattagatg ctaccatact
     5821 gctctttcag cttccatcaa catttccaga gaagaatatg aataatatga gtgctgtgct
     5881 taaatctttt catgatacaa ctgtaagatt tgctgttgag gcaagaaact taagtttaga
     5941 gaagaggaat gaatttgcta tggaaacaga tacaatacca gttatagacc cgtttactgc
     6001 tatagatagt tcattcagac ctcgagttat acataatact tcatacttca gattgcatgg
     6061 ttcacctcca ggtaaagtga tgtatgggta tgactataca gactgtgatc tagagagact
     6121 cttatcctat atccagcctg taaaagattc taatgtctat gtactgttta ataataccga
     6181 gatgtataag aacgcattga ggtttaagga gatggtttca tagatggtag aggcgatgtt
     6241 ttatgagaag cttaaagata gtctggtccg atgcaccctc tgtcccagat actgtgttat
     6301 aaagccagag gagaggggat actgtggtac tagacttaat aaggatggta tactctatgc
     6361 ggtaagctat ggggtggtaa gttccatagc caatgaccct gtagagaaaa aaccgctttt
     6421 tcatttttat cccgggtcta tgg
//
LOCUS       DAOTKU010000030         6394 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_11369_length_6394_cov_3.111059, whole genome shotgun sequence.
ACCESSION   DAOTKU010000030 DAOTKU010000000
VERSION     DAOTKU010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6394
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11369_length_6394_cov_3.111059"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="P5310_01365"
     CDS             complement(<1..288)
                     /locus_tag="P5310_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32045.1"
                     /translation="MSIIFMLALSILLVAVLLLSNQEIYATQVCLNGNKAFYYAEAGV
                     EYAQAQLLNGKSVDELNQPSVDNSLKEKLDSRISSLNLQVENPSGSKYTLTC"
     gene            687..1361
                     /gene="rpe"
                     /locus_tag="P5310_01370"
     CDS             687..1361
                     /gene="rpe"
                     /locus_tag="P5310_01370"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF004076.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HRU32046.1"
                     /translation="MPKDKIEVSPSVMCMNFRNLEEDFRILENAGADRFHFDIMDGHF
                     VPNITLGQDILRALRPVSKLPFEAHLMVENPERFIDRFIDADIITFHLEATKAPVRLA
                     RSIKELKKIVSVAINPVTPPELLKFLLCEIDEVLVMTVEPGFAGQKFIPQIIEKVRIL
                     KGLISRENPSVRIEVDGAISAEIGRDLRSAGAEIFVGGTSGIFMPDGNLNENLIRFKK
                     ALSECI"
     gene            1392..2438
                     /locus_tag="P5310_01375"
     CDS             1392..2438
                     /locus_tag="P5310_01375"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent dehydrogenase"
                     /protein_id="HRU32047.1"
                     /translation="MKAAIFESLENMIIKDDVPEPELPNDGLVIKIESVAICGSDVRI
                     FHRGHTKVNPPQIIGHEVAGTVYKVGKETSEFKEGERVVLGADVPCGKCYYCLRGHQN
                     NCAKAIAFGYQLQGGFTQFMAVPREAIDAKIIQRLPDDLSFDEACLTEPLACVVNGQD
                     LSNIQAGDTVAIIGAGPIGLMHAMLARAKGATKIIMSELSDKRLEYARNFNIDILINA
                     KDEDPVERILKETDSMGADIVIVAAPSGSAQAQAIHMVRNLGTVNFFGGLPPNTKEIP
                     IDTNWIHYREIFVHGSHGSSPKDNAIALKLISTGQVEAKKLITHILPLDKINEGLKLV
                     ESGEALKVVLKPWG"
     gene            complement(2557..3072)
                     /locus_tag="P5310_01380"
     CDS             complement(2557..3072)
                     /locus_tag="P5310_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRU32048.1"
                     /translation="MDVYDAIKTRRSVRAYQDKPVPEDVLKRILEAARLAPSASNRQD
                     WKFIVVRDSGNRRAVAQAANNQMFIAQAPVIIVAVSLNPDRIMSCEVPSYAVDLAIAV
                     DHITLAATAEGLGTCWIGAFSQREMKKVLDIPERYKVVTLTPLGYPADSPRPKVRKPL
                     EEIVCYEKFKE"
     gene            3156..4499
                     /locus_tag="P5310_01385"
     CDS             3156..4499
                     /locus_tag="P5310_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32049.1"
                     /translation="MKYLTFITVVIFTILSVGTLSYAGEGVELFRLRVVNDYSGPVEV
                     SLDGGSSWMMLGKVDRPALIFNPDAFRASGYIENGTVAATAVNAIHIKVGDSGADPVN
                     TSEKSGRTISIMPRGTDQSKEPNSRILTNIEGGTLIFKEWSPFVGNKVLLEKGDELIR
                     IPDDYTPSIGDRIVIPVTAPESAPREILFENRLDGNVYLCYSDAEREPVAKVLRPVKG
                     TGRFGGTLFALPSSVRANHPGVIDISTSNVFSGEYSEAPLEEAGGFQIIPSRHANTPE
                     LKGVWTSTQWMIVGPIDENFLEGAYPLFRGLIRPGYDVDGKFGDSDVWVKLPISKGLN
                     NYALSDLREFRIVLPPLEIVPPEETVNPPEISSIKINIQDKTAEIEGTADPGVKIQII
                     VNDKIMGECYSKADGFFSAKNIPVHYRNLITCKAIDPAGREGRIGKRISVVIPED"
     gene            4504..5640
                     /locus_tag="P5310_01390"
     CDS             4504..5640
                     /locus_tag="P5310_01390"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRU32050.1"
                     /translation="MKELTSRERLLRVFNGKIPDRIPISLYEFDGYYDSWIHNYPEYV
                     EILEYARGKTDKMSPWYPESDRPVLFYGEIDRENIESLSWKEDKSVYTRRIIKTPRGE
                     LSSTTRQDEGVHTSWTIEHLCKDEADAEKVLSLEYIPWKPSTDSFFKKVDEIGESGIV
                     IGDIPDALCLTVELFGFYEFIMLYTDNPAIIFKLIEFFQERLYNYLSHILSNGVVTAY
                     RIVGPEYATPPYLSPEDFKKLVAGYDKEIIDLLHKYGGKARLHSHGKVKRVLETISEM
                     GIDAIDPLEPPPDGDITLKEARDILGDRVVMIGNIEERLFEVGTKEDIESAVKTAIEE
                     GASGGPFILCPTAMPLTTPLDKMIQENIIHYIDCGIKYGKEKKQ"
     gene            complement(5691..6095)
                     /locus_tag="P5310_01395"
     CDS             complement(5691..6095)
                     /locus_tag="P5310_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32051.1"
                     /translation="MEPEINLSVEFADNAIKLIVENTGSIPVSDLELFITEGKISQIE
                     EYWIKNPSRVVKLKTIPSFSPKRKEIFSLPLLEEDSDSDICQEIMIRYRDNTGSVYED
                     GYVTSLLIDTEKAKISLSRSEGKPDKRKRKSK"
     gene            complement(6097..>6394)
                     /locus_tag="P5310_01400"
     CDS             complement(6097..>6394)
                     /locus_tag="P5310_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32052.1"
                     /translation="ELTYSSNTGKIYSLNGGSYMKSCFRVLFLKDVKDIRLSFDSFAS
                     LVVVGGKGIITSQDGEFPIERGETYIILAGFGEHRYIGEEPLEIIVSLPPQGVR"
BASE COUNT         1973 a         1259 c         1407 g         1755 t
ORIGIN      
        1 acaggtaagg gtatattttg aaccactggg attctctacc tgcaaattta aagaacttat
       61 cctgctatcc agcttctctt ttaaagaatt atccactgaa ggttgattta gctcatccac
      121 actcttcccg ttcaaaagct gcgcctgggc atactccacc cccgcctctg catagtaaaa
      181 agccttattt ccattaagac aaacctgagt ggcatatatc tcctggtttg aaagtagaag
      241 caccgccacc agaagtatag acagggcaag cataaatata atactcaaaa tgagggttat
      301 cccctctctg cctttcactc tcctcacccc ttattattaa ataatttaca ggttaatttt
      361 agcacaacta taaataaata caatagcagt cttcactgtc aatagtctgt gaaaatgtca
      421 gggaaggaaa taaaacatat gagattaatt tgtatcccgt aggggcaatt catgaattgc
      481 ccctacaatt tcgtctttta taataaaaac accatacctt tatccttcct cccccatctc
      541 ggaggaggat agaggtggga gaggggaata ggtcagaact atttacccat gacaatatca
      601 cagaacgata gcagtcttta cttgacaata tgccagagta tcttaaaatt aagagaaggg
      661 tctggcttta aattactgga gggaatatgc caaaagataa aatagaagta agtccatcgg
      721 taatgtgtat gaactttagg aatctggaag aggattttcg tattctggag aatgctggag
      781 cagatagatt tcactttgac attatggatg gacattttgt ccccaatata actttaggtc
      841 aggacatatt gagggcactg agacctgtaa gtaaacttcc ttttgaagct catcttatgg
      901 ttgaaaaccc ggagagattt atagatagat ttatcgatgc ggacataatt acattccatc
      961 tggaggctac aaaggctcct gtgaggcttg ccaggagtat aaaggaatta aaaaagatag
     1021 tatcggtagc tataaatcca gttacccctc ctgaattgct taaattctta ctctgtgaga
     1081 tagatgaagt cctggttatg acagtggaac ctggatttgc aggacaaaaa tttatacctc
     1141 agatcattga gaaggtaaga atacttaagg ggttgataag tagagagaat ccatcagtaa
     1201 gaatagaggt agatggagca atcagtgcag agataggaag agatttaagg tctgcagggg
     1261 cagaaatatt tgttggtgga acctccggta tatttatgcc tgatggtaat ctgaatgaaa
     1321 acttgatcag gtttaaaaaa gcattgagtg aatgtatctg aagaacaatc cagaaaggtg
     1381 gtgttaattc tatgaaagca gcaatatttg aatcgctgga gaatatgata ataaaggatg
     1441 atgtgccgga gccggaatta ccgaacgatg gtcttgtaat caagattgaa agtgtagcca
     1501 tatgcggctc tgatgtaagg attttccaca gaggacacac taaagtcaat cccccccaga
     1561 ttatagggca tgaagttgcc ggtacagttt ataaggtggg aaaggaaaca tctgagttca
     1621 aagaaggcga gagggtagtt cttggtgctg acgtcccctg tggtaaatgc tactattgct
     1681 taagaggtca tcagaataac tgcgccaagg cgatagcatt tggttatcaa cttcagggtg
     1741 gatttaccca gtttatggct gtaccaagag aggctataga tgcaaagata atacagagat
     1801 taccagatga cctgtccttt gacgaagcct gccttacaga gccactggcc tgtgtggtaa
     1861 atggacagga tttatcaaat atacaggctg gagataccgt tgccataata ggggcaggac
     1921 ctattggctt gatgcatgca atgttagcca gggcaaaggg tgcaactaag attataatga
     1981 gtgaactttc cgataagaga ttggagtatg ccagaaactt taatattgat atcctcatca
     2041 atgcaaaaga tgaagaccca gtagaaagaa tccttaaaga gacagatagt atgggagctg
     2101 atatagtcat agtagctgct ccatcaggtt ctgctcaagc tcaggctata catatggtca
     2161 gaaatcttgg cacggtaaac ttttttggag gacttcctcc caacacaaag gagataccga
     2221 tagatacaaa ctggattcat tatagggaga tttttgttca tgggagtcac ggttcttctc
     2281 caaaggataa cgccatagca ttgaaactta tatccaccgg gcaggtggag gcaaaaaaac
     2341 ttataaccca tatactcccg cttgataaga ttaatgaggg attaaagtta gtggagtctg
     2401 gagaggcttt aaaggtggtc ttaaagccct gggggtaatg ggagctcttt cgccctctcc
     2461 cttgacagaa gatctctttc tccctctccc ttgacgggaa gctttctttc tccctctccc
     2521 ttgacgggag agggtggggg tgagggtgag aaacttttat tctttgaact tttcgtagca
     2581 gactatttct tccagaggct tccttacctt tggtcttgga gagtcagcag gataccctaa
     2641 cggtgtaagg gtcaccacct tatacctctc cggaatatcc aggacctttt tcatctctct
     2701 ctgagagaat gccccaatcc agcatgttcc aagtccctct gcggtagctg ccagagtaat
     2761 atggtcaact gcgattgcca ggtccaccgc ataagaagga acttcacagc tcataatcct
     2821 gtctggatta agactgaccg ccactatgat aaccggagcc tgggctataa acatctgatt
     2881 gtttgctgcc tgtgcgacag ctctcctgtt acctgaatct ctgaccacga taaacttcca
     2941 atcctgtctg ttacttgccg atggggcaag tcgggctgcc tccagtatcc tcttcaggac
     3001 atcttcaggc acaggcttat cctgataagc tctcacactt cttctggttt taatagcatc
     3061 atagacatcc ataatctgca cctcctgctt tgaactttct atcagttttt gatatataat
     3121 tagtatacac tataaaccat aggagggata aaatcttgaa atatttaact tttataactg
     3181 tcgtaatctt tacaatctta tcggttggga cattatctta tgccggtgag ggggtcgaat
     3241 tattccggct cagagtggta aatgattatt ccggtcctgt tgaggtaagt ttggatgggg
     3301 gaagcagctg gatgatgctg ggcaaagtgg atagacctgc attaattttt aacccggatg
     3361 ctttcagagc cagtggttat atagagaatg ggacagtagc tgcaacagct gtaaacgcca
     3421 tacatataaa ggttggagat agcggggcag accctgtaaa tacatcagaa aagtccggaa
     3481 ggactataag tattatgccc agaggaacag accaatccaa agaacctaat agcagaattc
     3541 taaccaatat agaaggtgga actctgattt tcaaagaatg gtcacctttt gttggaaata
     3601 aagttctcct tgagaaggga gatgaactta tccggatccc cgatgactat actcctagta
     3661 ttggagacag gatagttatt cccgtcactg cccctgagtc tgcgcccaga gagatactct
     3721 ttgaaaatag gttagatgga aatgtatatc tctgctacag tgatgctgaa agagagccag
     3781 tagcaaaggt cctccgaccg gtaaagggga ctggaagatt tggagggaca ctttttgccc
     3841 ttccgagctc ggtaagggca aaccatccag gggtgataga tatatccaca tccaacgtct
     3901 tcagcggtga atattctgaa gccccactag aagaggcagg aggatttcag attataccta
     3961 gcaggcatgc caacacccct gaacttaaag gggtatggac atccacccaa tggatgatag
     4021 ttggacctat agatgaaaac ttcctagaag gggcgtatcc tcttttcagg ggtcttatca
     4081 gaccgggata tgatgtggat ggtaagtttg gagatagtga tgtatgggta aaacttccaa
     4141 tttcaaaggg attaaataat tatgccctat ctgacctgag agaattccgt attgttttac
     4201 caccactgga aattgttccg cctgaagaaa ctgtgaaccc tccagagatt agctctataa
     4261 agataaatat tcaggataaa acagcagaga ttgaagggac agccgaccct ggtgtaaaga
     4321 ttcagataat tgtaaatgat aagataatgg gagaatgcta ttccaaggct gatggctttt
     4381 tctctgcaaa aaacatacct gttcactata gaaacctcat aacctgtaag gctatagacc
     4441 ctgcgggtag agaaggcaga ataggaaagc gaatcagcgt tgtgatacct gaggactaaa
     4501 aatatgaagg agttaacatc cagagagaga cttttaagag tttttaatgg caaaatcccc
     4561 gataggattc ccatttcact ctatgaattt gacggatatt atgattcatg gattcataat
     4621 tatccagagt atgtagaaat cctagaatat gcaaggggta agacggataa gatgtcccca
     4681 tggtatcctg agtcagatag acctgtcctc ttctatggag aaatcgatag agaaaacata
     4741 gaaagtctca gctggaagga ggataaatct gtatatacca gaaggataat aaagacacca
     4801 agaggagaac tctcatccac aacaaggcag gatgaaggag tacataccag ctggacgata
     4861 gaacatctat gcaaggatga ggcggacgca gaaaaggtcc tctcactaga atatattccc
     4921 tggaaaccat caacagattc tttttttaaa aaggtagatg agattggcga gtcaggtata
     4981 gtcataggtg acataccgga tgcgctatgc cttaccgtag aacttttcgg cttttacgaa
     5041 tttataatgc tctatactga taatccagcg atcatcttca aacttataga atttttccag
     5101 gaaagactgt ataactatct atctcacatt ctatcgaatg gagttgtcac cgcatatagg
     5161 atagttggac cagagtatgc aaccccacca tatctgtctc cagaagattt taaaaaactg
     5221 gtggcagggt atgataaaga gattatagac ctgctccata aatatggtgg taaagcaaga
     5281 ctccattctc atggaaaggt aaaaagagta ctggagacca tatcggagat gggtatagat
     5341 gctatagatc cgttggaacc tccgccagat ggggatatca ccttaaaaga ggcaagagac
     5401 attctgggtg acagggtggt catgataggg aatatagagg aaagactatt tgaagtgggg
     5461 acaaaagaag atatagagag tgcggtaaag actgcaatag aagagggagc cagcggaggt
     5521 ccatttatac tctgtcccac cgccatgccg cttacaactc cactggataa aatgattcag
     5581 gaaaacataa tacactatat agactgcgga attaagtatg gaaaagagaa gaaacaataa
     5641 gggtgagata tcacaggtct cacccttatt gcagatagaa atactgttac ctatttagat
     5701 tttctttttc tcttatcagg ttttccttca ctcctactca ggcttatttt tgctttctct
     5761 gtgtctataa gaagagacgt tacatatccg tcttcataga cactacctgt attgtctcta
     5821 tatctaatca taatctcctg gcatatatct gagtctgagt cttcctccaa cagtggtaaa
     5881 gagaatatct ccttccgttt aggggaaaat gagggtatag tttttaattt taccaccctg
     5941 gacggattct ttatccaata ctcttctatt tgagagattt taccctcagt aataaaaagt
     6001 tctaaatcgc tgaccggtat tgaacctgta ttttctacaa tcagctttat agcattatct
     6061 gcaaactcta cagagagatt aatctcaggt tccatactac cttacgcctt gcggaggtaa
     6121 agatactatt atctccagag gttcttctcc tatatatctg tgctcaccaa aaccagccag
     6181 gattatatat gtttctcccc tctctatggg aaactctcca tcctgagatg ttataatccc
     6241 cttccctcct acaaccacaa gagatgcgaa actatcaaat gaaagtctta tatcttttac
     6301 atccttcaag aacaagaccc tgaagcagga cttcatatag cttccaccgt tcagactgta
     6361 gatcttacca gtattcgaac tgtatgtgag ctcc
//
LOCUS       DAOTKU010000031         6243 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_11644_length_6243_cov_3.477214, whole genome shotgun sequence.
ACCESSION   DAOTKU010000031 DAOTKU010000000
VERSION     DAOTKU010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6243
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11644_length_6243_cov_3.477214"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="P5310_01405"
     CDS             <1..478
                     /locus_tag="P5310_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007280238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HRU32053.1"
                     /translation="GWSRAWMISLWARLKEGDLAYESLKKLLTDFTADNLFDLHPPII
                     FQIDGNFGAVAGIAEMLLQSHRDIIELLPALPTDWKDGYVRGLCARGGFEVDIGWKAG
                     RITEAKIRSKNGGLCKIESRNPFTVICDGEKIILKNPGTYLYSFPTEEGRSYILDF"
     gene            complement(524..1372)
                     /locus_tag="P5310_01410"
     CDS             complement(524..1372)
                     /locus_tag="P5310_01410"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HRU32054.1"
                     /translation="MMKLSVCHYSFHRRWEEEKWTLERLCQEVKSLGVNSVDFHARLV
                     SRLGTPDDIVQEIKTTISKTGIDLSGFSLSTNFNIENSQEYQDMIKDTIVWMQVAAKI
                     GAPVSRVFGGGLRNRLNIDEKTKRDIFQRVIDALGVLSKEAEKLGLILALENHGGLPC
                     TGEEQVEMIEKVGSKYLMATVDIGNYLQCGQEAVDGVRIASKYCAYVHAKDFKKKPDS
                     SMPWGWNVSPCIVGEGDVDVAGCLSVLKEAGYDGYIALEYEGLEDERIGVPKSLEYVK
                     SVMDRI"
     gene            complement(1395..2435)
                     /locus_tag="P5310_01415"
     CDS             complement(1395..2435)
                     /locus_tag="P5310_01415"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRU32055.1"
                     /translation="MNNNEPIQVGVVGLGRSGWNIHIRTFRNMTDKFRVVAVCDINEA
                     RLEEAKTSLGCKTYARYEDFLGDQEIELVVVATPTYLHPPYSIMALKSGKKVVCEKPM
                     ATTTKDADEMIRVSKETGSLLTVFQNRRYGKDHIKVKEVIASGKLGRIVQIRMTMNSF
                     GRRWDWQTLKEFGGGSLNNNGIHLLDRALQLMSEKEPEVFCHLDRALTLGDADDHVKI
                     ILKAPGEPLVDIELTSACAYPIESWLVMGTCGTLIGTPSELKWKYIKPEELSPRAVDK
                     EPTPDRSYNREDLHWYEETWKEEDSLITQEMAFYTDLYRTIREGAPLAVKPEEVRKRI
                     AIIEKCHKMNNL"
     gene            complement(2458..3474)
                     /locus_tag="P5310_01420"
     CDS             complement(2458..3474)
                     /locus_tag="P5310_01420"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HRU32056.1"
                     /translation="MRRKIICFPERGKVAIKEEELSEDLENDEILIENLKNLISPGTE
                     LALYTGTHVGFKDPKNLWAKYPHYPGYISIGRIVKTGKCVVGLNPDDIVLSSMNHCSH
                     GKLKDSDPLLIKLPENADLDTLLFARMAAISMTAPLLTDYSIGDRIAIIGLGIVGNLC
                     SQLFQLSGADVYPLEIVPYRIKIAQNAGINRVLNGGETHEVKSQIQQVTNELGVDIVV
                     EATGIPELVNQALELVNNLGQVILLGSTRGLVNIDVYTHIHHKGITVKGAHANVLDST
                     KIVGEPNGMRKYILRMIDLILSGRLKVYPLITHRIAPEDVEDAYNWLLKKENMALGII
                     INWK"
     gene            complement(3543..5513)
                     /locus_tag="P5310_01425"
     CDS             complement(3543..5513)
                     /locus_tag="P5310_01425"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRU32057.1"
                     /translation="MKRFIERLALVCLVISLVLNLAPNVFSQQASIGQYSTIEEYERA
                     TGKKITKFSESPMLADLVKQGKLPSVEKRLPEEPIVIQPIEEVGKYGGTWRRAALSPN
                     DTRIRDRMGYEPLVKWGRDGKTMVPNLVKSWELTNRGKTYVFYLRKGVKWSDGEPFTA
                     DDIMFWYNDVILNKELTPLFPAWLASGDKPVEVVKRDDYTVEFRFAAPYSIFLNYLAG
                     PDTGVFITNYPKHYLQKFHPKYTPKEKLDSLVKEAKFDSWFQLFADKANPNTNPELPT
                     IRPWVLKSNPTATRLVAERNPYYWKIDTVGNQLPYIDKVAFDIVQDTQMAVMKAVSGE
                     LDMQGRHMSLADYTLLMENRTKGNYDVYLWNEGKAGTALYINQNYKGDEDITKLFRNV
                     KFRTALSLAINRDEINALVYLGQGRPCYVMFPFTSLQNDPNIRKLYEYNVAEANRLLD
                     SLRLNRRNKDGFRLLPNGKPLQVTLLVGLGYPMHPDVSELVKTYWEKVGVKTAIDTVS
                     QDLWWSRVQANNYQIVPHYLEFCEGFLDMSYSSMSFIPCRALTYFAPLWGLYYESLGK
                     NGEKPTGDALRSQMLFDKLKTSTSKDEQDKLTEELLRVWAQNLWAIPTAGSYKIPIVV
                     KKNFKNVPKNASLAYSYCSPGYLNPEQFFFEE"
BASE COUNT         1765 a         1368 c         1047 g         2063 t
ORIGIN      
        1 tggatggagt agggcatgga tgatatctct atgggcaaga ttaaaggagg gagatttagc
       61 atatgagtca ctaaagaagt tgttaactga ttttacggct gataatctct tcgatcttca
      121 tccccccata atatttcaga tagatggaaa ttttggcgct gtagcaggaa ttgctgagat
      181 gttattgcag agccataggg atataataga actccttcct gcgctgccta ctgattggaa
      241 ggacggttat gtaaggggct tatgtgccag aggtggtttt gaagtagata taggatggaa
      301 agctgggcga atcacagagg ctaaaataag gtctaaaaat gggggattat gtaagataga
      361 aagtagaaat ccgtttacag ttatctgcga tggagaaaag ataatactta aaaatcctgg
      421 aacttatctt tattcatttc ctaccgagga aggaaggtcc tatatcttag atttttagtg
      481 acataaaaca acaggagaca gggcgggaat ccccgccctg ttattagatt ctatccataa
      541 ctgatttaac atattcgagg ctttttggaa ctccaatcct ttcatcctca agcccttcat
      601 attctaaagc tatataaccg tcatagcctg cctctttaag cactgagagg caaccagcca
      661 catcaacatc gccctctcca acaatacagg gagaaacatt ccatccccag ggcatggaag
      721 aatccggttt tttcttaaaa tccttcgcat gaacataggc acaatattta ctcgctatcc
      781 taacaccatc aaccgcttcc tgtccacatt gcagatagtt gcctatatct actgttgcca
      841 ttagatattt tgaccctacc ttttctatca tctctacctg ttcttcacct gtgcagggaa
      901 gtccgccatg gttttccaat gctaatataa gccctaattt ctctgcctct ttagatagaa
      961 ctcctagagc atctattaca cgttgaaaga tatcccgttt tgtcttctca tcgatattta
     1021 atctgttcct gagacctcca ccaaagaccc tagagacggg agctcctatc ttagctgcta
     1081 cttgcatcca tacgattgta tcttttatca tatcctgata ttcctgtgag ttttctatat
     1141 taaaattggt agaaagagaa aacccggata agtctatgcc tgtcttagag atagtcgtct
     1201 ttatctcctg cacgatatca tctggagtcc ctaacctgct gacaagtctt gcatgaaaat
     1261 ccaccgagtt aacacccaga gactttacct cttggcagag tctctctaat gtccattttt
     1321 cttcttccca tcttctatga aaactatagt gacatacaga taatttcatc atctgcctcc
     1381 ttattctctc ttaattagag attattcatc ttatgacatt tctctattat ggctatacgt
     1441 ttcctgactt cctctggctt tacagctaga ggagcaccct ctcgaatcgt gcgatataga
     1501 tctgtataaa atgccatctc ctgcgttatt aagctatcct cctctttcca tgtctcttcg
     1561 taccagtgca gatcctctct attataactt ctatcaggag tgggttcttt atccacagct
     1621 ctgggggata actcttcagg ttttatatac ttccatttta attctgaagg ggttcctatg
     1681 agagttccac aggtccccat gacaagccag ctctctatgg gataggcaca tgcacttgta
     1741 agttcaatat ctactaaagg ctctccaggg gctttaagga taatcttcac gtggtcatct
     1801 gcatctccaa gtgttagagc tctatccagg tgacagaata cctcaggctc tttctctgac
     1861 ataagttgaa gtgcacgatc taagagatga atgccattat tattaagaga ccctccgcca
     1921 aattctttta gtgtctgcca gtcccacctg cgaccaaaac tattcatggt cattcggatc
     1981 tgtactatgc gtccgagctt accagaggct atgacctcct ttaccttgat atgatcctta
     2041 ccataacgtc tattttgaaa tacggtaagg agagaccccg tctctttaga aactctaatc
     2101 atttcatctg catctttagt tgttgtagcc ataggcttct cgcaaactac tttcttgccg
     2161 gatttaagag ccattataga atagggtgga tgcagatagg ttggagtagc taccacaact
     2221 aattctatct cctgatcacc cagaaaatct tcgtatctgg catatgtttt acatcccaag
     2281 gatgtcttag cttcttctag tctagcctcg ttaatatcac agacagcgac aacacgaaac
     2341 ttatctgtca tatttctaaa ggttcggata tgtatattcc atccgctacg tccaagccca
     2401 actactccca cctggattgg ttcgttgttg ttcatcttta tctcctcctt atataaatta
     2461 tttccagtta ataattatcc ccaaagccat attttctttc ttcaatagcc agttataagc
     2521 atcttcaaca tcttctggag ctattctatg agttatgaga ggatatactt ttagccttcc
     2581 agatagtata agatctatca ttcttaggat atacttgcgc ataccattag gctctcctac
     2641 tattttagtt gaatctaata cattagcatg agctccttta acagtaattc ccttatgatg
     2701 aatatgagtg taaacatcta tattaactag tccacgggta gaacctaaaa gtattacctg
     2761 ccctaggtta ttaactaatt ctaacgcctg atttactaat tctggtatac ctgtagcttc
     2821 aacaacaatg tccaccccca attcattggt tacttgctga atctgggatt tgacttcatg
     2881 agtttctcct ccgttaagga ctctatttat ccctgcattc tgagctattt taatacgata
     2941 tggaactatt tccaatggat atacatcagc cccagataat tggaatagtt gagagcatag
     3001 attcccaaca atccccaatc ctataatagc tatcctatca ccaatagaat agtctgttaa
     3061 tagaggagct gtcatactga tagcagccat cctggcaaaa agtaatgtgt caagatcagc
     3121 attttcagga agcttgatca aaagtgggtc tgagtctttt agttttccat gcgaacagtg
     3181 attcatgcta gaaagtacta tatcatctgg attcaatcct actacacatt taccagtctt
     3241 tactattctc cctatcgata tataaccggg atagtgcgga tatttagccc ataaattttt
     3301 tggatcttta aaacctacat gtgttccagt atagagagca agttctgtac ctggactaat
     3361 taagtttttt agattctcaa ttaaaatttc gtcgttttct aaatcttctg ataattcttc
     3421 ttcttttata gctaccttac ctctttctgg gaaacatata atctttcgtc tcatatacaa
     3481 atctcctcca taatgtatca agtaagccaa gacgaatcct ggcttacttg ataattgcct
     3541 aattactctt caaagaagaa ttgctcagga tttaaataac ccggactaca gtacgaataa
     3601 gccaagcttg catttttagg gacattctta aaattctttt tgactacgat cggtatctta
     3661 tagcttccag cggttgggat tgcccaaagg ttttgagccc atacccttag aagctcttca
     3721 gttaacttat cctgttcatc tttactcgta ctggttttta atttatcaaa gagcatctga
     3781 cttcttagag catctccggt aggtttctcc ccattctttc ccaaactttc atagtataaa
     3841 ccccagagcg gagcaaaata tgtcagggct ctgcagggga tgaaagacat acttgaataa
     3901 ctcatatcaa gaaatccctc acaaaattct agatagtgag gtactatttg gtaattatta
     3961 gcttgaactc gtgaccacca taaatcctga gacactgtgt ctattgctgt tttaacccct
     4021 actttttccc aataagtttt tactagctct gagacatcag gatgcattgg ataacccaag
     4081 ccgacaagaa gtgttacttg gagtggtttc ccgtttggga gtaatctaaa tccatcttta
     4141 ttacgtctat taagccttag actatccaaa agtctattag cctcagctac attgtactca
     4201 tataatttgc gaatatttgg atcattttgt agtgaggtaa aaggaaacat tacatagcat
     4261 ggtctccctt gccccagata tacaagtgca tttatttcat cacggttaat ggcaagggaa
     4321 agggctgtgc ggaattttac atttcgaaag agcttagtta tatcttcgtc tcccttgtag
     4381 ttttgattta tataaagagc agttcctgct ttcccttcat tccataggta gacatcatag
     4441 ttccccttag ttctattctc cattagtagc gtataatcag caagagacat atgccttccc
     4501 tgcatatcta gttcaccaga tacagctttc ataactgcca tctgtgtatc ctggactata
     4561 tcaaaggcaa ctttatcgat atagggaagt tgattaccaa cagtatcaat cttccaatag
     4621 taaggatttc tttctgctac taatcgagta gcagtaggat tacttttaag tacccatggt
     4681 cttatagtag gaagttcagg attagtattg ggattcgctt tatcagcgaa tagctgaaac
     4741 catgagtcaa atttagcttc ttttactaaa gaatctagct tttctttcgg agtatatttt
     4801 ggatgaaatt tttgtagata atgttttgga taattggtta taaatactcc agtatccggt
     4861 ccagctagat aatttagaaa tatagaataa ggcgcagcaa aacgaaactc tacagtatag
     4921 tcatctctct ttactacttc taccggctta tctccagacg ccaaccatgc aggaaataag
     4981 ggggtaagtt ctttgttaag gataacgtca ttataccaga acattatatc atctgctgta
     5041 aaaggttccc catctgacca tttaacccct tttctaagat aaaagacata ggtttttcct
     5101 ctattggtaa gctcccaaga tttaactaaa ttaggaacca ttgttttccc gtctcttccc
     5161 cattttacta ggggttcata tcccatccta tccctgattc tagtatcatt cggtgataat
     5221 gccgcccttc tccaagtccc accgtatttc ccgacttctt ctattggttg tatcacaata
     5281 ggttcctcag ggagccgctt ctctacagat ggaagttttc cttgtttaac aagatcagca
     5341 agcattggac tttcactgaa ttttgtaatt ttcttaccag tagccctttc atactcttct
     5401 atagtagagt attgtcctat agaagcctgc tgagagaaaa catttggtgc tagatttagc
     5461 actaaagata taaccagaca gactaaagct aatctttcta taaatctttt cataaatatg
     5521 cctcctcact taagattact tttttataaa cttctacccc tttggggtta acgagactta
     5581 gaagttatcc cttctattta tctatcgctc tttttatctc atctattacc tcctctacaa
     5641 gactcataag attttcggaa actctaaaac tccttgtagt cttagatcta ggataataat
     5701 cttattttcc cccacagccc aaacctcttc acccttactt tttcctctaa atatcaccta
     5761 tctttctctc aagcaggatt ttaagaaggc agtaaacatg cttaaatctc aacacttggc
     5821 acagcattta ttataccaga aaaatctcct caattcacta aaatatactg atcttcggac
     5881 aggagttcgg ccagctttct aaattgtata tctgaatcct aattataggt atcctaaagt
     5941 ataaatttat atcaaatctt accttctaat acgagtatgt attaaagtaa catggtatgg
     6001 ctcttccttt atatggaaca ctgacacaaa ttatggcaaa tcctcttgtc ttaccatcac
     6061 ttagtactcc aacgtaagag gtatgatgag cttcttttct tggtatttct gtagggtcta
     6121 caataacaaa aggaacttta cccaagttgc gctataagta ccagaaatca gcctgtaagg
     6181 gtctgtcttt atagtttaaa gcctgatata ttggctacct cttcttctgg ctcactacac
     6241 tgc
//
LOCUS       DAOTKU010000032         6212 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_11700_length_6212_cov_3.223161, whole genome shotgun sequence.
ACCESSION   DAOTKU010000032 DAOTKU010000000
VERSION     DAOTKU010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6212
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11700_length_6212_cov_3.223161"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="P5310_01430"
     CDS             <1..531
                     /locus_tag="P5310_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRU32058.1"
                     /translation="DMMVNRALGRAEVIAKHGGLDGFALCSDYCFNTGPFLSPNLFSE
                     FITPYLYKLTKGYRDMGFYVIKHTDGNIMPILDQLVQTNPHAIHSLDPQGGVDIREVK
                     RLVGDKVCLIGNVNCGLLQTGTDEEVIESVRYALKYGMPGGGYIFSTSNCIYTGMPLT
                     RYELMLEIWRKEGIYE"
     gene            622..1056
                     /gene="mraZ"
                     /locus_tag="P5310_01435"
     CDS             622..1056
                     /gene="mraZ"
                     /locus_tag="P5310_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004821298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HRU32059.1"
                     /translation="MIFLGEVRHVLDEKGRIVIPSRFRPALKDGLCITRGFDNALFIF
                     PMEEWIKIDAKLRTSRMADKELRKFMRFWYSGAVQDGLDSQGRLLIPRNLREYAMIDR
                     EIVIIGVSNRLEIWSAELWDKYSGDSKEFYRTLGEALNDIDI"
     gene            1040..1921
                     /gene="rsmH"
                     /locus_tag="P5310_01440"
     CDS             1040..1921
                     /gene="rsmH"
                     /locus_tag="P5310_01440"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HRU32060.1"
                     /translation="MISISSFAHKAVMLDEVLNVLLVKPGGRYIDCTLGSGGHSYAIL
                     DASNPDGILVGIDMDPYAIDEARRNLSFYGDRFIPIQGNFVNLIELVSRTGIDKFDGI
                     LFDLGISSIQLDNSERGFSFNKDGPLDMRMDPSIKITAHQIVNSFPMEKLESIFREFG
                     EERSSYKIARAIVEYRKKKEISTTKELADLISSVVKRKTKIHPATRVFMALRIYINNE
                     LDNLRNALEQAVCLLKPGGRLCVISYHSLEDRIVKRFFKGSGCRIIGKSVITPTRDEI
                     LVNRRARSAKLRGLEKL"
     gene            1918..2274
                     /locus_tag="P5310_01445"
     CDS             1918..2274
                     /locus_tag="P5310_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32061.1"
                     /translation="MSKNLARESPYSERQADVNLKPASIQNKTKYFLARIVFISIMVT
                     SIVAFSLFQVKLYSKVISYRQEITALEDTITDFSIKNEELRREAEVLSSPARLREEAE
                     RLGLQPSDRLLKIPLN"
     gene            2277..3776
                     /locus_tag="P5310_01450"
     CDS             2277..3776
                     /locus_tag="P5310_01450"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HRU32062.1"
                     /translation="MNIRRLEILKIIFIAVFIVEGGALFYWQIIWNPESSFYFDEEDR
                     SVERGKIYDRRGRIIAMNELRYSIFADPSIVSDPEYVGKKIGELLGRPEEIYINRLKQ
                     KSRFVWIERKLEKSLAERIVSIDRGGIFMREDYCRVYLDCNMEEILGCVDIDNRGISG
                     IELTLDERLSRGEDVYLSIDSVLQSKIDDLLSGNKQVLGFSKGIVIVMSPYTGEILAL
                     VNYPHDRNFAIQDVFEPGSSFKPIVASIALEEKCVREDERFNCRPLFIIKGVSIKDAP
                     HQVDSYDMTLKEILEVSSNIGIAQIGLRIGPSIFYRYVSAFGFGSYTGVELSGESPGI
                     LPQRRDEISITQNSFGQGIGVNALQLVAGYAPLANGGYFVRPTILKVSSSDRILNQII
                     SGETSKKITGMLVNVVENGTGRSAKIDGVEIAGKTGTAQKVVNGRYSMENNIMSFIGY
                     LPAYCPKFIIGVILDEPKVSRWASDTAAPLFKKIAEILLYDESLKGDEM"
     gene            3773..5242
                     /locus_tag="P5310_01455"
     CDS             3773..5242
                     /locus_tag="P5310_01455"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HRU32063.1"
                     /translation="MKILKLIEGIDISEMRNIKDIEKIEVNNISSNSKDIKESGLFIA
                     IRGEHFDGHNFIEEAIKRGALLALGEEDIKEFPYIMVPDSKRAYAFISSNYYNNPFRE
                     IKTVGVTGTNGKTTTVNLIGSILETLGEKAGIIGTLGARLGSEELVTQFTTPEQLLLA
                     ELYNRARNLGINKIVMEVSSQSLAQHRVDAVRFEAGIFTNLTQDHLDFHKDMESYFLS
                     KRRLFEMVSEDKDGIMAINIDDDYGKRLVQEFSSYNLITYAIKNSAMIMAKDIEILPY
                     GLSFNLLAEGRTFKVSSKLLGEVNVYNILSAISYIYGRKLPLDRAIEVIHNFQPVRGR
                     LENVNLGQPFRVLIDFAHTPDALERVLKISKNFGKRVIVVFGCGGDRDRFKRPLMGNI
                     ATSLSDIAIITSDNPRSEDPMSIIEEIVKGVSRNNYIIKEDRREAIHYAIDIAEPEDV
                     VLIAGKGHETYQIFKDRTIHFDDREVATEAIKRRIDNAH"
     gene            5232..6176
                     /gene="mraY"
                     /locus_tag="P5310_01460"
     CDS             5232..6176
                     /gene="mraY"
                     /locus_tag="P5310_01460"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HRU32064.1"
                     /translation="MPINTLASFFLSLVLSIIGFKIYLKYFKSFLPKYVRDYGPKSHL
                     AKQGTPTMGGAVIWLSTLITLLILAPNREIIASLLLITGYAFIGGIDDYLGIKKKSSQ
                     PSKARYKFAIECIITLIWLLTVKHFSIFDLKADFLLFHITSTFWIYVFETLFIVGMAN
                     AVNETDGVDGLAGTITSILLVFIFMMFKDTNLSLLTLALLGGLSVFLFYNAHPAKLIM
                     GDVGSLSLGSSIAVMGIIVGKEWVVLILGVVFLIEAFSVMLQVPYYKLTKKRIFKMTP
                     IHHHFELSGWSEPQIVTRFSLLTLIAAILGVFINGPKW"
BASE COUNT         1996 a          906 c         1431 g         1879 t
ORIGIN      
        1 gatatgatgg tcaatagagc cctggggaga gctgaggtta tagctaaaca tggaggatta
       61 gatggttttg ccctttgttc agactactgt ttcaacacag gaccattctt aagtccaaat
      121 ctattttctg agtttataac tccttattta tataagttaa caaagggtta tagggatatg
      181 ggattttatg ttataaaaca tacagatgga aatataatgc ctatcctgga tcagcttgtt
      241 cagaccaacc cccatgcaat ccattcactt gaccctcagg gaggggttga tataagagag
      301 gtcaaaaggc tggttggaga taaggtctgt cttataggta atgttaactg tggactttta
      361 cagacaggaa cagatgaaga agttatcgaa tctgtccgtt acgccctgaa gtatggtatg
      421 ccaggtggag gatatatctt ttctacatct aactgtatat atacagggat gccattaacc
      481 cgttatgaac ttatgctcga gatatggaga aaagagggaa tatatgagta attttagata
      541 tatgtagtcc atctcctatt gaaatttttt atcccaggtg ctagtatagt taaagtggta
      601 tgaagtggag ggttgtggta tatgattttc ctgggagaag taaggcatgt attggatgag
      661 aaggggagga ttgtaattcc ttcaaggttt agaccagctt taaaggatgg tctttgtata
      721 accagagggt ttgataatgc cttatttata ttcccaatgg aagaatggat taaaatagat
      781 gcaaaactca gaacctcacg gatggctgat aaggaactac gtaaatttat gagattctgg
      841 tactctggag ctgttcagga tgggttggat tctcagggga gattgttaat ccccaggaat
      901 ctccgtgaat atgcgatgat agatagggaa atagtaataa ttggagtatc gaacagattg
      961 gagatctgga gtgcagaact ttgggataaa tattccggag attctaaaga gttttataga
     1021 actcttggag aagcgttaaa tgatatcgat atctagtttt gcccataaag cggttatgct
     1081 ggatgaggtt ttaaatgtcc tgcttgtaaa gcctggtggc aggtatatag attgtactct
     1141 ggggagtggt gggcacagtt atgccatcct ggatgcgagt aatccggatg gtattcttgt
     1201 cggaatagat atggatccat atgctataga tgaggcaaga agaaaccttt ctttttatgg
     1261 agatagattt atacctatcc agggaaactt cgttaacctc atagagttag tttctagaac
     1321 tgggatagat aagtttgatg gaatactctt tgatctgggg atatcttcta ttcaactgga
     1381 taattcagag agaggcttta gttttaataa agatggtccg ctggatatga ggatggaccc
     1441 atctatcaag ataactgctc atcagattgt taactccttt cctatggaga agttagagtc
     1501 tatttttaga gaatttggag aagagagatc atcttataaa atagcgagag ctatagtgga
     1561 gtatcgaaag aaaaaggaga tatctaccac taaagagctt gcagatctga tcagttctgt
     1621 ggtaaagaga aaaaccaaaa tacacccggc taccagggtc tttatggctt tgaggattta
     1681 cataaataat gaattggaca acctgaggaa tgctcttgaa caggcagtct gtcttttaaa
     1741 accgggtgga agattgtgtg ttatctctta tcattctctg gaagatagga tagttaagag
     1801 attctttaag ggaagtggat gcaggattat tggaaagtca gtaataactc ctaccagaga
     1861 tgagatttta gtgaatagaa gggctagaag tgctaaatta agggggttag agaagttatg
     1921 agtaaaaatc ttgccagaga aagtccatac tcagagcgcc aggcagatgt taatttaaag
     1981 ccagcttcta ttcaaaataa aactaaatat ttcttagcca gaatagtctt tatctcaatt
     2041 atggttacat ctatagttgc tttcagttta tttcaggtaa aactatacag taaggttata
     2101 agttatagac aggaaattac tgccctagaa gataccatta cagatttcag tattaagaat
     2161 gaagaactga gaagagaagc tgaagtttta tcctctcctg ctcgtcttag agaagaagcg
     2221 gagagacttg ggcttcagcc aagtgataga ttactaaaaa tacctctaaa ttagccttga
     2281 atatacgaag gctggaaata ttaaagatta tttttatagc ggtctttata gttgagggag
     2341 gagcactctt ctattggcag attatctgga atccagagag cagtttttat tttgatgaag
     2401 aggatagatc ggttgaaagg ggaaagatat atgaccgcag aggaagaatt atagctatga
     2461 atgaactacg atactctata tttgctgacc cttctattgt aagtgatccg gaatatgtgg
     2521 ggaaaaagat aggtgaatta cttggcagac cagaagagat atacataaac aggttgaaac
     2581 agaaatctcg attcgtctgg atagagagga agctggagaa aagtctggct gaaaggattg
     2641 tatctataga caggggcggt atatttatga gagaagatta ttgccgtgta tatctggatt
     2701 gcaatatgga agagatttta ggttgtgtgg atatcgataa cagaggaata agtggtatag
     2761 agttgacttt agatgagaga ctatcgagag gagaggatgt atatctgagt atagatagtg
     2821 ttttgcaatc taaaatagat gatctattgt ctggaaataa gcaggttctg ggtttcagta
     2881 agggtatagt gatagttatg tctccatata ccggagaaat cctggctctt gtaaactatc
     2941 cacatgatag aaactttgcc atacaggatg tttttgagcc aggttcatca ttcaagccta
     3001 tcgtagcttc aattgccctg gaggagaagt gcgtcagaga ggatgaacgg tttaattgta
     3061 gacctctatt tataataaag ggtgtttcca taaaagatgc acctcatcag gtagattctt
     3121 acgatatgac cttaaaagag atcttagaag tctcatctaa tataggaata gcacagatag
     3181 gactcagaat aggtccatct atattctata gatatgtgtc tgcctttggc tttggatcat
     3241 acacaggggt ggaattatca ggagagagcc cagggatatt gcctcagagg agagatgaga
     3301 taagtattac tcagaatagt tttggtcagg ggataggggt aaatgcctta cagcttgtag
     3361 ctggttatgc gccgctggct aatggtggct attttgtgag acccaccata cttaaggtct
     3421 cctctagtga taggatcctt aatcagataa tatccggcga gacttcaaaa aagattaccg
     3481 gtatgctggt aaatgtagta gaaaatggga ccggaagaag cgctaaaata gatggggtag
     3541 agatagctgg caagactggg accgcccaga aggtggtaaa tggacgttat tctatggaaa
     3601 ataatattat gtcttttata ggatatctac ctgcctattg ccctaaattt attataggtg
     3661 taatattgga tgagcctaag gtaagcagat gggcttccga tacagcagct ccactgttta
     3721 aaaagatagc agagattctg ttgtacgatg aatccttaaa aggagacgag atgtgaaaat
     3781 attaaagtta atagaaggta tagatatatc agagatgaga aatataaaag atattgaaaa
     3841 gatagaggta aataatataa gcagtaactc taaagatatt aaagaaagcg gtctttttat
     3901 agctataaga ggggaacatt ttgatgggca taattttata gaggaagcta taaaaagagg
     3961 agcactacta gctttaggag aagaagatat aaaagagttc ccatatataa tggttcctga
     4021 tagcaagaga gcctatgctt ttataagcag caattactat aataatccct tcagagagat
     4081 aaaaacagta ggtgttacag gaaccaatgg taaaaccacc acagtaaatc ttattggcag
     4141 tatacttgag actttagggg aaaaggctgg aataattgga accttagggg caagattagg
     4201 atctgaagag ctggttaccc aatttacgac acctgagcag ttacttctgg ctgagctcta
     4261 taacagagcc agaaatttgg gtataaataa aattgtaatg gaagtttcct cacaatcatt
     4321 agcacagcac agggtagatg ctgttcgctt tgaagctgga atatttacta atcttaccca
     4381 ggaccatctg gatttccata aagatatgga atcgtatttt ctctctaaga gacgtttatt
     4441 tgagatggta tctgaggata aagatggaat tatggcaata aatatagatg atgattatgg
     4501 aaaaagattg gtccaggagt tctctagtta taatcttatt acatatgcta taaagaactc
     4561 tgctatgatt atggctaaag atatagagat tttaccttac ggactttctt ttaatcttct
     4621 tgctgaaggc agaacattta aggtttcttc caaactctta ggagaggtaa atgtttataa
     4681 tatactctca gccataagtt atatatatgg aagaaagctc ccattagata gagctattga
     4741 agtgatacat aactttcagc ctgtaagggg taggttggag aatgtaaacc tggggcaacc
     4801 ttttcgagta ttaatagatt ttgcccatac cccggatgca ttggagaggg tcttaaaaat
     4861 ttctaaaaat ttcggtaaga gggtaattgt tgtgttcggt tgcggtggag atagggatag
     4921 atttaaacga cctcttatgg gaaatattgc aaccagttta tccgatattg ctataattac
     4981 atcagataac cccaggagtg aagaccctat gagtataata gaggagatag tgaaaggtgt
     5041 aagtagaaat aactatataa taaaagaaga cagaagagaa gctattcatt atgctataga
     5101 tatagcagaa cctgaagatg tagtattgat agcaggcaaa gggcacgaga cttatcagat
     5161 atttaaggac aggactattc attttgatga tagagaggtg gctacggaag ctattaaaag
     5221 gaggatagac aatgcccatt aatactctgg cttcattctt tttatctctg gtattgagta
     5281 tcattgggtt taagatctat cttaaatatt tcaagtcttt tctccctaaa tatgtcagag
     5341 actatggtcc caagtctcat cttgcaaagc agggaactcc aactatgggt ggagcagtga
     5401 tatggttaag taccctgata acgttattaa ttttggctcc aaatagggag attatagcat
     5461 cgctgttact tataaccgga tatgccttta ttggtggtat agatgattat ttgggtataa
     5521 agaagaagtc atcccagcct tcaaaagcca gatataaatt cgctatagag tgtatcatta
     5581 cattgatatg gcttttgaca gttaaacatt tttccatatt tgatctgaag gcagactttc
     5641 ttttatttca tataaccagt actttctgga tctatgtatt tgagacttta tttatagttg
     5701 gtatggcaaa tgctgtaaat gaaacagatg gggtagatgg attagccggt accatcacat
     5761 ccatactttt agtttttatt tttatgatgt ttaaagatac taatctatct cttttaactc
     5821 tggcattgtt gggcggactg tctgtatttc tattctataa tgcacatcct gcaaaactca
     5881 ttatgggtga tgtcggttct ttatctttag gctccagtat agcagtcatg ggaataatcg
     5941 tcggtaaaga atgggttgta cttatcctgg gtgtagtatt tctcattgaa gccttctcgg
     6001 ttatgttaca ggtcccatat tataaactta caaagaaacg tatattcaaa atgaccccta
     6061 tacatcatca ttttgaactt tcaggctgga gtgaaccaca gattgttacc aggttctccc
     6121 tgttgacctt aatagcagcc atactggggg tatttataaa tggacctaaa tggtaaaaaa
     6181 gttacaatct tgggatatgg aaagaccggc aa
//
LOCUS       DAOTKU010000033         6120 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_11894_length_6120_cov_2.845837, whole genome shotgun sequence.
ACCESSION   DAOTKU010000033 DAOTKU010000000
VERSION     DAOTKU010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6120
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11894_length_6120_cov_2.845837"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..487)
                     /locus_tag="P5310_01465"
     CDS             complement(<1..487)
                     /locus_tag="P5310_01465"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HRU32065.1"
                     /translation="MKGFKRLFFLSMLLVLVVSLFSGVTFAQDKNVLDTLKGENTASI
                     FLEAVEMANLTDILTEGEGVTVLVPTDDVADDLKAAMDEGDTDKIYSIVSCHIISSEI
                     LTASDLSSAGEVYTDGGMITVKQEDNDIIINDTIKIVKADIKAKNGIIHLIDGVILPQ
                     EE"
     gene            complement(569..1738)
                     /locus_tag="P5310_01470"
     CDS             complement(569..1738)
                     /locus_tag="P5310_01470"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRU32066.1"
                     /translation="MDKGTRTKIQHYGEDYSKFLGAISPPIFEASNFAFSSYEEVEEH
                     QEGKLKDKFTYTRGGNPTVRILENKIAMLEKGDACRAFSSGMGAITASILSLIKGGDH
                     IVCLRGVYNPAYEFIDSYLSRFNVSSSFVEGKDLSDIESAIRPNTRVIYLESPNSLIF
                     EVFDLREIARMARERGIYTIIDNTWATPYFQNPIEYGIDVVVHSASKYLGGHSDVVAG
                     LVITSKELIKGVSRELNLIGSIIGPFEAWLILRGLRSLPSRMELHQNNAMKVAQYLEN
                     HPRVIKVNYPGLPSNPQHQLAISQLKGFSGLMSFELDGGEKELRKFVNNLKLFAIAVS
                     WGGYESLIFPTGRGFPKDRKDKVKISPQLLRISVGLEDAEDLIEDLENAFKKAFN"
     gene            complement(1751..2038)
                     /locus_tag="P5310_01475"
     CDS             complement(1751..2038)
                     /locus_tag="P5310_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRU32067.1"
                     /translation="MEIIREILARGGNLMLTRNTFPVKGTKAEKHNHYHEQVSYILSG
                     SFKFTLNGEEYILKVGDSIYIGSNVPHSAESLEDNSVVIDVFTPQREDFLK"
     gene            2208..2801
                     /locus_tag="P5310_01480"
     CDS             2208..2801
                     /locus_tag="P5310_01480"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRU32068.1"
                     /translation="MEEINTRERIIRSAMECFSKYGYDGTSVERICESARISKGSFFY
                     YFPTKQSLFLEILSRWLEDVDRNMGEVVKADEDIRIKLLNLSMSLIDILTTGEKEISL
                     TFEFLNRALHDEETFKRIISMFESYRNYFSSLLEEDIKKGRIINIDPKIVSYTIVSFA
                     VGLLVQRLFSVDDTDWVTIAREGLEIIIKGLIDNSVI"
     gene            2841..4022
                     /locus_tag="P5310_01485"
     CDS             2841..4022
                     /locus_tag="P5310_01485"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRU32069.1"
                     /translation="MGIRLVGKEDYEQVFYLDAYCFPWLNDVRDRAKDFVLKHIPEDA
                     ILGYYDDSGMLNAMLFILPFSIYIYGSPVGMGGIASVSSMPEGRHGGKVTQLLRRSIE
                     IMRERKEFVSMLGPFSYEFYRRYGWELGFERLNYTIPVEHFSNFTRKTGYVKPYREGD
                     VEILDKIYSEYARKHNGCTVRDKTLWTDFVLEDPYGRDYPKYTYIWFDDGNNARGYII
                     YSIKNNRMTIQEMIYLDQAAKEGLFWFIFVHQSQIKEVYWSTAPDERLYLDIPNPRVK
                     MEISPSMMFRVIDVKHALLSRGYDGDVNARFSISISDPNAEWNNKGFLIEIEDGHIDI
                     GETESPESSCSIQTFSQIFTGYITPEEACMHRKLTGDPKTIREMGMVFKKSSTFNNNP
                     F"
     gene            4060..4971
                     /locus_tag="P5310_01490"
     CDS             4060..4971
                     /locus_tag="P5310_01490"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HRU32070.1"
                     /translation="MNAYPFNGSGNWYKGNLHCHSTVSDGALTPQEVADLYRKNGWNF
                     LALTEHNIYTDWQEFNREDFIIIPGVEVSVDEKERPRCYHLVGLSDGTKSLPNKESIR
                     GYEWNGPSSVQDTIDKLNEKGLYVVLCHPIWSRTEFEDFKDFGGYFGIEIYNHGCEME
                     NHTGFSTVYWDSLLRRKRRVWGLATDDAHHKIKDHLGGWVMVKAESLTRSSIISALLD
                     GRFYSSIGPSIYDFGVEDDEVFIECSEVKAIHFVTYETLGWSHYAGDNNLLTSSRFKL
                     KGKELYVRVECIDEYGRVAWTNPIFLK"
     gene            5029..5625
                     /locus_tag="P5310_01495"
     CDS             5029..5625
                     /locus_tag="P5310_01495"
                     /inference="COORDINATES: protein motif:HMM:NF014373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HRU32071.1"
                     /translation="MLGGIVGWERETVNKPAGLRTHIILCLGSTLITIVSTTLPGSDP
                     GRIAAQIVSSLGVLGGLVVFREGHHAVTGITTAVSMWFMGGVGIAIGAGYYLPAIIST
                     LAIYIILVFLKSIEGKILGHGTGKTLTIKALNRPGLIGDIGKILGSHNVDIADLKLQI
                     SDDIAEIVLRVNCPQKFEPSSMLREIQGLEDIRSIGWE"
     gene            complement(5626..>6120)
                     /locus_tag="P5310_01500"
     CDS             complement(5626..>6120)
                     /locus_tag="P5310_01500"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRU32072.1"
                     /translation="ISFMPVWGFSMAATTLVGQNLGASRPDLAEEYVRRILKIGMLIV
                     CVLGSINFIFSSQLVSIYSPDAEMIQIGNFAIKILSLSLPFSCLVSILSASLRGAGDI
                     IYTTITTFIGIWGFRLTLVLLFSKFFNLNIYGVWIAVGIDFAIRGLLYLARFKRGNWK
                     KGVV"
BASE COUNT         1829 a         1155 c         1282 g         1854 t
ORIGIN      
        1 cttcttcctg tgggagtatc accccatcta taagatggat tatcccgttc ttagccttta
       61 tatcagcctt tactattttt atcgtatcgt taattattat gtcgttatct tcctgcttga
      121 cagtgatcat tcccccgtcg gtataaactt ctccagcact gctcagatca ctggcagtaa
      181 gaatctcaga tgagatgata tggcagctga caatactgta aatcttatcg gtatcaccct
      241 catccatagc tgctttcaaa tcatcagcca catcatctgt gggaaccagg actgtaactc
      301 cttctccttc tgtcagaata tcggtgaggt tagccatttc tactgcctca agaaagattg
      361 aggcagtatt ctctcccttg agtgtatcca gcacgttctt atcctgggca aaggtaactc
      421 cggaaaatag agacactacc agtaccagta acatagacag aaaaaacaat cttttgaaac
      481 ccttcataat aaatccctcc tctcttggac ttttatatag ctctatatta tagaataata
      541 tatattaaaa agacaatagt ttcttatatc aattgaatgc ttttttaaag gcattctcta
      601 aatcttctat taaatcctct gcatcttcaa gccctactga tattcttaat aactgcggag
      661 atatctttac cttatctttt ctgtcttttg gaaatcccct tcctgtaggg aagatcagac
      721 tttcatatcc tccccagctt acagctatgg cgaataattt gagattattt acaaattttc
      781 ttaactcttt ctctcctcca tcaagttcga aactcatcaa tccgctgaaa cctttgagct
      841 gtgatatggc taactggtgc tgaggatttg atggtagacc aggatagttc acctttatta
      901 cccgtggatg attctccaga tactgggcta ccttcatagc attgttctgg tgaagctcca
      961 ttctactagg taaagacctc aaacctctga gaattaacca tgcttcgaat ggacctatta
     1021 tactgcctat tagattgagc tctctactga ctccttttat tagctcttta cttgtaatta
     1081 ccaaccctgc taccacatcg ctgtgtccac cgagatattt acttgcagaa tggacaacta
     1141 catctatacc atactctata ggattctgaa aatatggagt agcccatgta ttatctatta
     1201 ttgtgtatat acctctctct ctagccattc tggcaatttc tctaaggtca aagacttcga
     1261 atataagact gttgggactt tccagatata taactctggt gttgggtctt atagcggatt
     1321 caatatcaga taagtcctta ccttcgacaa aagatgagga gacattaaac ctggatagat
     1381 aactatctat aaactcatat gcaggattat aaacccctct tagacatact atgtggtctc
     1441 ctccctttat taacgacagg atacttgcag ttatagcccc catcccggaa gaaaatgccc
     1501 tgcaggcatc acctttttcc agcatagcaa ttttattctc cagtattctg actgtaggat
     1561 ttcctcccct tgtatatgta aatttatcct tcagtttccc ctcctggtgt tcttctacct
     1621 cttcgtagga ggaaaaggca aaattcgatg cctcgaaaat gggtggggat atagctccta
     1681 aaaatttgga ataatcttct ccatagtgtt gtatttttgt cctggtccct ttatccacaa
     1741 atgccttctt ctattttaga aaatcctctc tctgaggtgt aaatacatcg atcaccacag
     1801 agttatcctc cagagattct gcgctgtgcg gcacattaga gccgatatat atactgtctc
     1861 caaccttcag tatgtattct tctccgttaa gggtaaactt aaaacttcct gagaggatat
     1921 agcttacctg ttcatgatag tggttatgtt tctctgcctt tgtacccttt acagggaagg
     1981 tatttctggt cagcataaga ttgcctcccc tagcgagtat ctctcttata atctccatag
     2041 acatcttcct cctatttaaa agtaatcttc tgttttattc tactacaaat atcaagggtt
     2101 accgatttct tcctccccca tctcgggaga gggtgaataa acttgacttg atctttccag
     2161 tagagtataa ttggctatac cgaccagtcg gtatatagga gaatgatatg gaagagataa
     2221 acacaaggga aagaattata agatctgcaa tggaatgttt ttccaagtat ggatatgatg
     2281 gaaccagtgt agagcgtata tgtgaatcag ccaggataag taagggtagt ttcttctact
     2341 atttcccgac gaaacaatcc ctgttcctgg agatattgag cagatggctt gaggatgtag
     2401 acagaaatat gggagaagtg gttaaagctg atgaagatat aagaataaaa cttctaaatc
     2461 tatctatgag tttaatagat attttgacca ctggagaaaa agagatatct ctgacatttg
     2521 aatttttaaa cagagcactt catgatgaag agacctttaa gagaataata tcgatgtttg
     2581 aaagctacag aaattatttt tctagtttgc tggaagaaga tattaaaaaa ggcaggatta
     2641 taaatattga ccctaagatt gtttcttata ctatagtctc ttttgctgtg ggcttgctgg
     2701 ttcaaagact tttttctgtt gacgatacag actgggtgac tatagccaga gaaggattgg
     2761 agataataat caagggttta atagataatt ctgtgatata atctttcggt acggttaaga
     2821 gctgaagaaa ggagatagat atgggaataa gacttgtggg caaagaggat tatgagcagg
     2881 ttttttatct ggatgcctat tgtttcccct ggttgaacga tgtaagggac agggcaaaag
     2941 attttgtgtt aaagcatata cccgaagacg caatcctggg gtactatgat gattccggaa
     3001 tgctgaacgc aatgcttttt atccttccgt tcagtatata catctatggt tctcctgttg
     3061 gaatgggagg tatcgcatct gtctcttcca tgccagaagg aaggcatggc ggaaaggtta
     3121 cccagttact gagaagatca atagagatta tgagagagag aaaagaattt gtctctatgt
     3181 taggaccgtt ctcctacgaa ttttacagga gatatggctg ggaactggga tttgagagat
     3241 taaattatac aattccagtt gaacattttt ctaactttac caggaagaca ggatatgtta
     3301 agccgtacag agagggagac gtagagattc ttgataagat atactcggaa tatgcaagaa
     3361 aacataacgg atgtacagtc agggataaaa ctctctggac agattttgta ttagaagacc
     3421 catatggcag agattatcct aagtatacct atatctggtt tgacgatggg aataatgcca
     3481 ggggatacat tatatattca ataaagaata acaggatgac cattcaggag atgatatatc
     3541 ttgatcaggc agcaaaggag ggtttattct ggttcatctt tgtccatcaa tctcaaatca
     3601 aagaggtata ctggtcgacc gcccctgatg agagattata tctggatatc ccgaatccaa
     3661 gggtcaagat ggagatatct ccctcaatga tgtttagggt tatagacgtt aagcatgctc
     3721 ttctcagtag aggatatgat ggggatgtaa atgcaaggtt ttcaatatcg atatctgacc
     3781 ccaatgcaga atggaataat aagggttttc ttatcgaaat agaggatggt catatagata
     3841 taggggagac tgagtcccct gagagttcct gcagtattca gactttcagt cagatattta
     3901 caggctacat aaccccagaa gaggcctgta tgcatagaaa gctcacaggt gaccccaaaa
     3961 ctatcagaga gatgggaatg gtctttaaga agtccagtac atttaacaat aatccatttt
     4021 agttaatttt atcttaaaac aggaggcgat aaaaaaatta tgaatgcgta tccatttaat
     4081 ggaagcggta actggtataa gggtaatctt cactgtcatt caaccgtatc tgacggggca
     4141 ttgacccctc aagaggtggc ggatttatat cgtaaaaatg ggtggaattt tctggctctt
     4201 accgaacata acatatatac tgactggcag gaattcaata gggaggattt tataataata
     4261 ccaggggtcg aggtctcagt agatgagaag gaaagaccaa ggtgttacca tctagtagga
     4321 ctttcagatg ggactaaaag tttacccaat aaggaatcga taagaggata tgaatggaac
     4381 ggtcctagct ctgtgcagga taccatagat aaattgaatg aaaaagggtt atatgtagtt
     4441 ttatgccatc cgatatggtc caggacagag tttgaagatt tcaaggattt cggaggttat
     4501 tttggtatag agatatataa ccatggctgc gagatggaga accataccgg attctcaact
     4561 gtatactggg actcacttct gagaagaaag agaagagtct ggggtctggc taccgatgat
     4621 gcccatcaca agataaaaga ccatctcggt ggatgggtaa tggtaaaggc ggaaagtctg
     4681 accaggtcca gcattatatc cgccctttta gatgggagat tttattcctc aataggaccg
     4741 tctatatatg attttggagt tgaagatgac gaggtattta tagagtgctc tgaggttaag
     4801 gctatacact ttgtaactta tgaaacacta ggttggagtc actatgcggg tgacaataac
     4861 cttttaacct ccagcagatt taagttgaaa ggcaaagagt tatatgttag agttgaatgt
     4921 attgatgaat atggcagggt tgcatggaca aatcctatat tccttaagtg aggaggggaa
     4981 ttaatcctga gttttcttga gattctcctt aagtttttat tagccatact gctcggtggg
     5041 atagttggat gggagagaga gactgtaaat aagccggcag gtctcaggac ccatataatt
     5101 ctctgtttgg ggtccactct tatcactata gtatccacta ctctaccagg ttcagacccc
     5161 ggaagaatag cagcacagat tgtttctagt cttggggtac tggggggtct tgtcgtattc
     5221 agagaggggc atcatgcagt gacaggtatt accactgctg taagcatgtg gtttatggga
     5281 ggagtgggga tagctatcgg ggcaggttat tacctgccag caataatctc cactctggct
     5341 atttatataa tactcgtctt tttaaagtcc atagagggaa aaatcctggg gcatggaacg
     5401 ggtaagactc ttactataaa agctttaaat agaccaggac ttataggaga tataggaaag
     5461 atacttggct cacacaacgt ggatatagcc gatttaaaac ttcagatatc tgacgatata
     5521 gcagagatag ttttaagggt taattgtccc caaaaatttg agccttcttc aatgttacgt
     5581 gagatacagg gtctcgaaga tataagatcg ataggctggg aatagttata ccactccctt
     5641 tttccagtta ccccttttaa atcttgccag atataacagt cctcttatag cgaagtctat
     5701 acctacagct atccacacac catatatgtt gagattgaag aacttactga agagtaaaac
     5761 tagagttaga cggaaccccc atatgcctat gaatgtggtt atggtagtat atatgatgtc
     5821 ccccgctcca cgcagagacg ctgataatat ggataccagg catgagaacg gtagagagag
     5881 ggatagtatt tttatcgcaa agttacctat ctgtatcatc tcggcatctg gagaatagat
     5941 tgacaccagc tggcttgaga agataaagtt tatactcccc agtacacata ctataagcat
     6001 tcctattttt aatatccttc tcacatactc ttctgcaagg tcaggtcttg aagccccaag
     6061 gttctgtccc accagtgtgg ttgcagccat agaaaaaccc catacaggca taaatgatat
//
LOCUS       DAOTKU010000034         6094 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_11942_length_6094_cov_3.287465, whole genome shotgun sequence.
ACCESSION   DAOTKU010000034 DAOTKU010000000
VERSION     DAOTKU010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6094
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11942_length_6094_cov_3.287465"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="P5310_01505"
     CDS             complement(<1..462)
                     /locus_tag="P5310_01505"
                     /inference="COORDINATES: protein motif:HMM:NF014045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HRU32073.1"
                     /translation="MGRRALFIDGNNLYFVQRKLKWNVDFEKLLRYFRGESDFYNAFY
                     YSGYDVNDQNQENFLKALTLIGYTVRKKPLKTISSQDNEEKQKSNMDVEMTLDLILTA
                     SHYDESYLFSGDSDFEGVIEYLRVMGKEIVVVSTLGHIAIELRNAADKYIDL"
     gene            882..1802
                     /locus_tag="P5310_01510"
     CDS             882..1802
                     /locus_tag="P5310_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HRU32074.1"
                     /translation="MAFNAWLLDKFFPHREPSRKDIGYLEAWVSILGNIFLFIVKFLF
                     GLSLNSISLIADAFHSLSDVLTSIVVLFGFKMGSKPADKEHPFGHGRIEEIATLIIAI
                     LLLIVAYDLGRTSVERIIHPQPVETNILAVVFMMFSGLFKEWMARFSIFLGLKINSDT
                     LIADAWHHRSDAVASVLVGIGLLFTRYGYYYIDGILGLGVVALLVWVGIELLKTASDF
                     LIGRAPDKEFISRIRDIVLSVPGVLSLHDILVHDYQSEKIISLHIEVEGYLSAREAHR
                     IALEVQDRLRKSLDCSDVSVHIDPKGERED"
     gene            1870..3927
                     /locus_tag="P5310_01515"
     CDS             1870..3927
                     /locus_tag="P5310_01515"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HRU32075.1"
                     /translation="MNLLADFIVKRKTYILIVFLFLFVVSIFMMPDRKLSYNIFDILP
                     DDIESLIGLKILSEKIAKGPEFTILCEKDDLNEVENLVRKLETLPYIISISWLGTSQD
                     LTYPEEIWRDSASWYKDGVFKVSITLKSSDSYKTQIEQLRGLLPEWAELTGSEVISYD
                     MEDYFKNSTLIYFSLGILLVTVFLFLTFPSFLVPILIVFSMMIGVIINLAITAVSGRS
                     IYFLMDTIVAILQVAVTLDYALFLYHRYEEERLTKERDAAMASAIISSFKPISLSSLT
                     TMAGFFALTFGKLTIYSILGWILLRGVFISLIVIIFFFSSLLLLSDKFIAARKHRVIP
                     LGVGSLGRFMGRFSHIFSIIFLIFLAIFYYSYTRVEPIFDVNTFLPEDIPSIATLNKI
                     NSIFGQTESIIIVAEEDTTGFSDALSTIKNMDGVNTVIHYSTILDPAIPEELIPADVF
                     DRFRNNGYTFALVDINYKLNEENGKVLRDSIEKTIKEKVYGEAYITGESVLLADISDI
                     AYMDQSKTTRISLLLILIIIAIGFTSLSVPLVLVLVIQLAIWANIGYYFLFNIPMPFF
                     IPSILGAIQLGATIDYAVLLTSRYQEERRMGLSRIDASDRSVYWSSYSIITSAGTMIL
                     MNLPAAILSNIELVRITMGSLARGGFLSVMMVLFLLPALLVLLDKVFKYSSYKWSGDD
                     KNE"
     gene            3920..5296
                     /locus_tag="P5310_01520"
     CDS             3920..5296
                     /locus_tag="P5310_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32076.1"
                     /translation="MNRLKFLFISLLLITLSINSAFASNLSISDDETVYAITRPDGSL
                     EKTVVVDWIRVEGDGEYAIYDGGKDLNNVRKLLGDASVSFSDSRVKIEGRVNGTSDIY
                     YRGETKKPLPLSLNITYYLNGKRTTYQNAAGKKGTLRIEFTVKNRLQKRVRIGNDEED
                     VYVPFTAILNASFPSSRIRDIKISEGGSTIVAGSNINVTLMGMPQPDFKGWLEVSMDK
                     IELNPLQIAVIPSSPALAGLLEGLGDYIGSIEQMDQVLKFQQDLLKQMSQEIKVSIDE
                     KGSSIETSLDNVGKMDEILGKQEGMMDDMLKATGKMIDRNNRMRLLAEKMEDGKELLA
                     LLEEEKRELEGMKEKLTAMKGNNRELRQFFSLMPVDGIREIMNSLSRIQMSLNAIAEG
                     GFVGFVMIPGINMVRDGIKTAKEEIEKNKERMDVLEGLARNYDTFTGKPGNAKKSSVR
                     FIYYIKFE"
     gene            5380..>6094
                     /locus_tag="P5310_01525"
     CDS             5380..>6094
                     /locus_tag="P5310_01525"
                     /inference="COORDINATES: protein motif:HMM:NF016593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32077.1"
                     /translation="MRIDEFLSLADTHKVFPVYKEVLQDTETPIGLLSRVISYPYVFL
                     LESVERGEYLGRYSFLGWVPRNVYEVYDNPFSLLRKTLTQDVSPFSNLPRFWGGLVGY
                     ISYDAIRCLEKIPFSPEKEKIDLPLGMFIRPDFIVAFDHVKNKMTAITDVMIDGNESN
                     VYNSAEEKLSFALDILNLPRREMNNFDARNNNDIERCIPKETFEEMVKSAVEYIKMGE
                     AFQIVLSQRFLFDSSGIDPL"
BASE COUNT         1847 a         1069 c         1307 g         1871 t
ORIGIN      
        1 caggtctata tatttgtcag ctgcgtttct cagttctata gctatatgcc ccagagtaga
       61 tacaactact atctccttac ccattacgcg caggtattct ataactccct caaaatcact
      121 gtctcctgaa aacaggtagg actcgtcata atgagaggca gtaaggatga ggtccaatgt
      181 catttcaaca tccatattgc tcttctgctt ctcttcatta tcctgagagg agatggtttt
      241 taaaggtttc tttctaaccg tataccctat caatgttaga gccttaagaa aattttcctg
      301 attctgatcg ttaacatcat accctgaata atagaacgca ttataaaaat cactctcccc
      361 cctgaaatat ctgagtaact tttcaaaatc tacattccat tttaattttc tttggacaaa
      421 atagagatta tttccatcta taaataaagc ccttcttccc atataatgaa attttcctcc
      481 ttaaaggatt cattaaaaca attttaacct tctcagctat tctgtcaata gtctaagaaa
      541 atgtcagggg aaggaaatga aacatatgag attaatttgt atcctgtagg ggcaattcat
      601 gaattgcccc tacaatttcg tcttttataa taaaaacacc atgcctttat ctttcctccc
      661 ccacctcggg ggaggaagga taggaaaatt ctcattacct attactcatc acccgctacg
      721 cattacggca tttattttat ccatctggac ttgacaactt ggaaatctgg aataaaattt
      781 taattgagga agaacaggaa aaatcccggg ctcctctctg gagtagcagt acatctaact
      841 gcaggacttc ccaataattt atgttgaagg agggatatac tttggctttt aacgcctggc
      901 ttttggataa gttttttcct cacagggagc cttccagaaa ggatattggt tatctggagg
      961 catgggtaag tattttgggt aatatcttct tatttatagt taaatttctt tttggactga
     1021 gtctaaatag catctctcta atagcagatg cattccactc actttccgat gttctcacct
     1081 caatagtagt tctctttggc tttaagatgg ggagtaaacc ggcagataaa gagcatcctt
     1141 ttggtcacgg tagaattgag gagatagcca cattaataat agctatccta cttttgatag
     1201 tagcctatga cctgggcagg acatctgtgg agaggataat acatccacag cctgttgaaa
     1261 caaatatact ggcagtggta tttatgatgt tctccggatt atttaaagaa tggatggctc
     1321 gattttccat cttcctggga ttaaagataa attctgatac acttatagct gatgcctggc
     1381 atcacaggag tgatgcggta gcgtccgtgc tggtaggtat aggacttctc ttcaccagat
     1441 atggatacta ctatattgat ggtatactgg gtctgggggt ggtggctctt ttagtctggg
     1501 ttggtataga actgttaaag accgcttcag attttcttat aggaagggct cctgataaag
     1561 aatttataag tcgtataaga gatatagtcc tttctgtccc tggtgtcctg agccttcatg
     1621 atatcctcgt ccatgattat cagagtgaaa aaattatctc ccttcatatc gaggttgaag
     1681 gttatctatc agccagggag gctcacagga tagcattgga ggtacaggat agattaagaa
     1741 agagtctgga ttgctctgat gtttctgtcc atatagaccc taagggagag agggaagatt
     1801 gacatctatt atatgatata agtaactgat tatggagata tttaaacttt ttatgaatct
     1861 ggtaaaaata tgaatctttt agcagatttt atagtaaaaa gaaaaacata tatactgata
     1921 gtttttcttt ttctttttgt cgtttcaatc tttatgatgc cagacaggaa gctgagctac
     1981 aatatatttg atatattacc cgatgatata gaatctttaa ttggactaaa aatcctctcg
     2041 gagaaaattg caaagggacc tgagtttaca atcctctgtg agaaggacga ccttaatgag
     2101 gtagaaaatc ttgtaagaaa gctggagact ctcccatata taatctctat cagctggttg
     2161 ggaaccagcc aggacctgac ctatccggaa gagatctgga gagactctgc atcctggtat
     2221 aaggatggag tgttcaaggt tagcataacc ttgaaatcgt ctgattctta taaaactcaa
     2281 atagaacagt tgagaggtct tctaccagag tgggcggaac ttactggaag tgaagtaatc
     2341 tcttatgata tggaggacta ttttaaaaat tctaccctta tatacttctc tcttggaatc
     2401 cttcttgtca cagtatttct gttcctaacc tttccaagtt tcctggtccc tatccttata
     2461 gtgttttcta tgatgattgg agtcataata aatcttgcta tcaccgctgt atcaggaagg
     2521 tcgatctact tccttatgga tacgatcgtt gcaatcctcc aggttgcggt gactctggac
     2581 tatgcactgt ttctctatca tagatatgaa gaggaaagac ttactaaaga gagagatgca
     2641 gctatggctt ctgcgattat ttccagcttt aaaccgatat ccctgagctc tctcaccaca
     2701 atggctggat tctttgccct cacgtttggt aaattgacca tctacagtat cctggggtgg
     2761 atactgctga gaggggtttt catatcttta attgtgataa tattcttctt ttcttccctt
     2821 ttactcttat ctgataaatt catagctgcc aggaagcaca gggttattcc gcttggtgtt
     2881 ggaagtctgg gaagatttat gggtagattt tcacacatat tttctattat atttttaata
     2941 tttcttgcca ttttctacta ttcttatacc agagtagaac ctatattcga tgtaaatacg
     3001 tttttaccag aggatatacc ctcaatagcg acattaaata aaattaacag catctttggt
     3061 cagacagaga gtatcattat agtggcagaa gaagatacca ccgggttctc cgatgcgcta
     3121 tctaccatta aaaatatgga tggggtaaat acggtgatac attattccac catactggac
     3181 cctgccatac cagaagagtt gattccggcg gatgtctttg acagatttag aaataatggt
     3241 tatacatttg ctttggttga tataaactat aaactgaatg aagaaaatgg taaagtcctc
     3301 agagattcta ttgagaagac tataaaagag aaggtttacg gagaggctta tataactgga
     3361 gaatctgtcc ttttagctga tataagcgat atcgcctata tggatcagag caaaactaca
     3421 aggatatccc ttttacttat actgattatt atagctatcg gatttacctc tttgtcggtg
     3481 cctcttgttc ttgtactggt catacaatta gccatctggg ctaacatagg atattacttc
     3541 ttatttaaca ttccgatgcc gttctttatc ccctcaatac tgggggcaat ccagctcggg
     3601 gctactatag attacgctgt ccttttaaca agcaggtacc aggaagagag aagaatgggt
     3661 ttgtccagga ttgatgcctc tgacagatcg gtatactgga gctcatactc tataataacc
     3721 agtgcaggca ccatgattct tatgaatctt cctgcagcca ttttgagtaa tatagagctg
     3781 gtaagaatca ctatgggtag ccttgccagg ggtggattct taagtgttat gatggttctg
     3841 ttcctactgc cggcattact ggttcttctt gataaagtat ttaagtattc atcatacaaa
     3901 tggagtggag atgataaaaa tgaatagatt aaaattttta ttcatatcct tattattgat
     3961 tactctgagt ataaacagtg cctttgccag taatctatct atttctgatg atgaaacagt
     4021 ttatgctatt accagaccgg acggtagtct tgagaagact gtagttgtgg actggataag
     4081 ggtagaaggt gatggggaat atgcaattta tgatggtgga aaagatctca ataatgtcag
     4141 aaaactgctt ggagatgcca gtgtatcttt ttctgacagc agagtaaaga tagaaggcag
     4201 ggttaacggt acctctgata tatattacag gggagaaaca aaaaaacctc tccctctatc
     4261 ccttaatata acctattatt tgaatggaaa aaggaccaca tatcaaaatg cggcaggtaa
     4321 gaaggggaca ttaaggatag aatttacagt gaaaaacaga ttacagaaaa gggtcaggat
     4381 aggtaatgat gaagaagatg tttatgttcc attcacggct attctgaatg ccagtttccc
     4441 ctcttctagg ataagagata taaaaatatc agagggggga agtaccatag tggcggggag
     4501 caatataaat gtaaccttaa tgggtatgcc ccagcctgac ttcaaaggct ggctagaagt
     4561 gagtatggat aaaatagagt taaatcctct ccagatagct gtaattccat catccccagc
     4621 cctggcagga ctgttagaag ggctgggtga ttatattgga agcatagagc agatggacca
     4681 ggtccttaag tttcagcagg acctcttaaa acagatgtcc caggagataa aggtatctat
     4741 agatgaaaag ggtagcagta tagaaacctc tctggataat gtgggaaaga tggacgagat
     4801 attgggtaag caggaaggta tgatggatga tatgcttaaa gctacaggca agatgattga
     4861 tagaaataac aggatgagac ttcttgcaga aaagatggaa gatgggaaag aacttcttgc
     4921 gttacttgaa gaggagaaga gagaactgga aggtatgaaa gaaaagctaa ccgctatgaa
     4981 aggcaataat agagagctga ggcaattctt ctctcttatg ccagttgacg gtataagaga
     5041 gataatgaat agcctttcca ggatacagat gagcttgaat gcgatagcag agggtggatt
     5101 tgttggtttt gtaatgatcc ctggtatcaa tatggttcgt gatggaataa agacagctaa
     5161 agaagagata gagaaaaata aagagagaat ggatgtactt gaaggattag ccaggaatta
     5221 cgataccttt acaggtaaac ctggtaatgc aaagaagagc agtgtaaggt ttatatatta
     5281 tataaagttc gaataatttt tgacaaaagt tacaggtata tgttatactc ttaggcaact
     5341 ttagtttaga ttagattgag gaaggagagt tgagaaaaga tgagaataga tgagttctta
     5401 tctttagcgg atacccataa ggtattcccc gtgtataaag aggttttgca ggatactgaa
     5461 acccctatcg gtcttctatc aagagttatc tcttatccct atgtttttct cctagagagt
     5521 gtggagaggg gagaatatct tggcagatat tctttccttg gctgggtccc tcgcaatgta
     5581 tatgaggtgt atgacaaccc tttttctctc ttacggaaga cactgactca ggatgtgtct
     5641 cctttttcta atctccccag attctgggga ggattggtgg gttatataag ctatgatgcc
     5701 ataagatgtc tggaaaaaat tcctttttca ccggagaagg aaaagataga cctcccccta
     5761 gggatgttta tccgacctga cttcattgtt gcctttgacc atgtgaagaa taagatgact
     5821 gccatcactg atgtaatgat agatgggaat gagtcgaatg tctacaactc tgcagaagaa
     5881 aagttgtcct ttgcactaga tatccttaac ctgccgagga gagagatgaa taattttgat
     5941 gccaggaata acaatgatat agagagatgc atcccgaagg aaacctttga agaaatggta
     6001 aagagtgccg tagaatatat aaagatggga gaagcatttc agatcgttct ctctcagagg
     6061 tttctcttcg attcttcagg gatagaccca ctgc
//
LOCUS       DAOTKU010000035         6041 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_12046_length_6041_cov_3.392249, whole genome shotgun sequence.
ACCESSION   DAOTKU010000035 DAOTKU010000000
VERSION     DAOTKU010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6041
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12046_length_6041_cov_3.392249"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="P5310_01530"
     CDS             complement(<1..593)
                     /locus_tag="P5310_01530"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly(A) polymerase"
                     /protein_id="HRU32078.1"
                     /translation="MLSKDTLLSILSIPDVRLCVENAFKKGVHIYMVGGTLRDLLLGL
                     EIKDVDLVVESSVEKFLEYVESLNKKGRLLRRFYTFKFETSYDPVEHIDIAIARKETY
                     PKPGALPIVEPGTIEEDLYRRDFTINALAIPVYPDGKTGNIIDPLGGLKDLSNRVIRV
                     LHNRSFIDDPTRIFRAVRFSIRLGFSIDSDTERLMKEA"
     gene            complement(550..1794)
                     /locus_tag="P5310_01535"
     CDS             complement(550..1794)
                     /locus_tag="P5310_01535"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014183563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HRU32079.1"
                     /translation="MNAIDIIIKKRDGKSLSKEEIEFFIKNFYKREITDYQMSAWLMA
                     SFIRGLSKEETVNLISAILSTGKILDWEFPVIDKHSTGGVGDKLSLIVVPLLASLGYK
                     VAKLSGRGLGHTGGTIDKLESIPNFRTSLTLDEFKSLVEKIGCSIISQTEELVPVEKE
                     IYSLRDVTGTVESIPLITSSILSKKIAGGAKTIIFDVKVGSGAFMKDIKTAEELAIWL
                     KDIGETFGKSINVVISDMNQPLGNAVGNLLEVLEAIDTLKGNGPEDLTELAIETAGTI
                     ASKELLKNSLSNGMAFNKFLDMVRAQGGDISNLSPSAKRYDFIALDSGYIGRLDAYLI
                     GEAVRTLGAGRYRKEDRIDPNVGIILKKKMGDYVEKGEVLLEIYYNSEEKLSSSLRLT
                     ERAISINKDAVERYPLVYSIYS"
     gene            complement(1791..2579)
                     /locus_tag="P5310_01540"
     CDS             complement(1791..2579)
                     /locus_tag="P5310_01540"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015594829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HRU32080.1"
                     /translation="MEMVTEIVKFLKDRYLFNQKIGVILGSGLSVFSEQSLFSIDYRD
                     IPGMPLSTVPGHRGRMDIVKVGDQEVIVMRGRYHLYEGYSSKDVVLPVDILKGLGVDI
                     LVITNASGGIRETFMPGDIMLITDHINLTGENPLIGGPNFVDMKNAYSYRLREQLKGI
                     GKEIGLELKEGVYVGVKGPTYETPAEIRFFRTIGGDAVGMSTILEVIRARSLNMEVLG
                     LSSITNVHKEDTSPLTHEEVLDMGNRISEKLDILLREWIKRGYV"
     gene            2796..3383
                     /locus_tag="P5310_01545"
     CDS             2796..3383
                     /locus_tag="P5310_01545"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HRU32081.1"
                     /translation="MCYNKFMSTGWIEVICGSMFSGKSEELIRRVKRAEIARQKVQVF
                     KPAIDTRYEDREVVSHDGNKVSAYMVEKAEDIIAELLPDVQVIAIDEGQFFDNSIVEV
                     CQTLADNGKRVIVAGLDQDFRGEPFGPMPELLAVAEKVDKLQAICMVCGGPASRTQRL
                     INGEPAYYDDPTILIGAEDVYEARCREHHVVRRRK"
     gene            3385..4251
                     /locus_tag="P5310_01550"
     CDS             3385..4251
                     /locus_tag="P5310_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013626278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HRU32082.1"
                     /translation="MQIGGQAVIEGIMMRGPNSFSVAVRTQNNEVKVHKSSTNSILNS
                     KINKIPILRGFISLIDSLVLGIKALNISAEEYGGEEVKLSNLDIFIAMAMGIGLGILL
                     FIVFPAFIARIIQNSISSALVVNILEGLVRIGVFLVYIYSFSFIRDIRRVFEYHGAEH
                     KVIFTYEKGEPLTVENAEKNSRLHPRCGTNFLLIVMVVSILVFSLLGKQTLLWRIISR
                     IILIPVVAGISYELIRLASKYSNSKIAKIITAPGLALQYLTTREPSQDQIEVAIEAVK
                     SILALEGVPYVG"
     gene            4241..5308
                     /gene="prfA"
                     /locus_tag="P5310_01555"
     CDS             4241..5308
                     /gene="prfA"
                     /locus_tag="P5310_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HRU32083.1"
                     /translation="MWDKLEEIERRYMELEESLAKPDVIANQELFQSLAKTYNELGDV
                     VGIYRRYKQIDKEISDIKSLLDDPELGGMAEEELEKLEGEKEKLEEELRIKLLPKDYR
                     DERNVIMEIRAGTGGEEAALFAGDLLRMYLRYVERKGWKAEIMSSNPTDLGGYKEVIV
                     SIQGRGVYSRLKFESGVHRVQRVPVTESGGRIHTSTATVAILPEATDVEVEINPDELK
                     IDTFRAGGHGGQNVNKVESAVRITHIPTGIVVTCQDERSQYQNREKALRILRAHLLEI
                     KERERENEEAQLRKSQVGSGERSEKIRTYNFPQNRVTDHRIGLTLYNLQDVLDGALDE
                     LIDALIREYQIEQLNNLSLTR"
     gene            5305..>6041
                     /gene="prmC"
                     /locus_tag="P5310_01560"
     CDS             5305..>6041
                     /gene="prmC"
                     /locus_tag="P5310_01560"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HRU32084.1"
                     /translation="MKLIDAYRETIRLFRDKIPNFQLEALLILSLVLDKPKEYILAHQ
                     ESELSEAEVEKIRELIDKRLSGIPYAYLKKEKEFFGIRFSVEEGVLIPRPETETLVEI
                     VLKMGPFTKGLDLFCGSGIIGLTILYSNGCKEFTGIDMSQKCIDVSIANASRLGLINR
                     TRFIMADIRSLSLEEEFDLIVANPPYIPDDRWNELDREVKSEPREALLGGKEGLDFYP
                     EIAKVISKNLVRGGVFAVEIGGKDQVD"
BASE COUNT         1917 a         1155 c         1226 g         1743 t
ORIGIN      
        1 gcctctttca taagtctttc tgtatctgaa tctatgctaa atccaagtct tatgctaaac
       61 cttaccgctc taaatatacg ggtagggtca tctataaagc tcctgttatg aagtactcta
      121 atcactctat ttgataaatc ttttaaccct ccaagagggt caattatatt tcccgtttta
      181 ccatccgggt agacagggat agccagggcg tttatcgtaa aatctctcct gtataagtct
      241 tcctcgatag ttccaggttc tacaatgggt aaagccccag gcttgggata tgtttccttt
      301 ctggcaatag ctatatctat atgttcaaca ggatcataag atgtctcaaa cttgaatgta
      361 taaaatctcc tcagtaatct ccccttttta ttaagacttt caacgtattc cagaaatttt
      421 tctacagatg actcaaccac caaatcaaca tcttttatct ctaaccccag aagaaggtcc
      481 ctgagtgttc cacccaccat atagatgtga acaccttttt taaaggcatt ttccacacat
      541 aatcttacat caggaataga tagaatagac aagagggtat ctttcgacag catctttatt
      601 tatagatatc gccctttctg taagtcttaa actggatgat aacttctctt cactattata
      661 atatatctct aaaaggacct ctcccttttc tacataatct cccatcttct tcttaagaat
      721 tatccccacg ttgggatcta tcctgtcttc ttttctatat ctccccgccc ccaacgttct
      781 taccgcctct cctataagat atgcatccaa tctacctata tatccagaat ctaatgctat
      841 aaaatcatat cttttggcag atggagaaag attactgata tctccaccct gagccctcac
      901 catatccagg aatttattaa atgccattcc atttgaaagg gagtttttta acaactcttt
      961 ggaggctatt gtcccggcgg tttctatagc caattctgta aggtcttcag gtccgtttcc
     1021 tttcagagta tctatagctt ccagtacctc cagaagattc ccaacagcat tccccagagg
     1081 ctggttcata tctgatatta cgacgtttat acttttacca aaggtttctc ctatatcttt
     1141 aagccagatg gcaagttctt cagcggtttt tatatctttc ataaaagcac ctgaaccaac
     1201 ctttacatca aatatgatcg ttttagcccc tcctgctatc tttttactca atatactgct
     1261 ggtaataaga ggaatactct ccacagttcc tgtaacatct cttaaggaat atatctcctt
     1321 ctctaccgga acaagttctt ctgtctgact tattatgctg cagcctatct tttcaactaa
     1381 agatttaaac tcatctaaag tcaaagatgt cctgaaatta gggatagact caagtttatc
     1441 tatcgtacct ccagtatgac cgagccctct accagaaagt ttggctacct tatatccaag
     1501 agacgccaga agaggaacaa cgataagact gagtttatcg ccaacccccc ctgtcgaatg
     1561 cttatctata acaggaaatt cccagtccag aatcttcccg gttgaaagaa tggcagagat
     1621 cagatttaca gtctcttctt tagacaatcc tcttataaaa gaagccataa gccaggcact
     1681 catctgataa tcagtaatct ctctcttata gaaattcttt ataaaaaact ctatctcctc
     1741 tttactgaga ctcttcccat cccttttttt aattattata tctatcgcat tcatacatag
     1801 cctctcttta tccattctct caataagata tccagcttct cacttatcct gtttcccata
     1861 tctaaaacct cctcatgtgt tagaggagaa gtatcttctt tatgcacatt ggtgatactt
     1921 gagagcccca aaacctccat attaaggctc ctggctctta tcacctcaag aatagttgac
     1981 atgccaacag catctccacc aatagttctg aaaaatctta tctctgcagg tgtctcatat
     2041 gttggtcctt tcacacctac atacaccccc tcctttaatt ccagaccaat ctcttttcca
     2101 atccccttta gctgttctct taatctataa gaataggcat tcttcatatc aacgaagttc
     2161 ggtccaccta taagaggatt ttctcctgta aggtttatat ggtctgttat aagcattatg
     2221 tctcctggca taaatgtctc tcttatcccc ccacttgcgt ttgttatgac caggatatca
     2281 acccctaaac ctttaaggat gtcaacaggc aagacgacat ccttagaaga atatccttca
     2341 tacaggtggt atctgcccct cataactata acctcctgat ctccaacctt aacgatgtcc
     2401 atcctccccc tatgcccggg tacggtagag agaggcatcc caggaatatc tctataatct
     2461 atagagaaga gtgattgttc gctgaatact gataatccag aacctaaaat tacccctatc
     2521 ttctggttaa aaagatacct gtcttttaaa aatttaacta tctcagttac catttccata
     2581 tattcctcct tttcctatta tatcagaaaa tatataacag ttgactgatc agctataatt
     2641 caatgtggag acttgttttg actatgaagt ggaggaattt tttaaagttt tccacagtat
     2701 attttggtgg tagaaaaaat caaacttatc cacagataat agataaaata ctttaccagt
     2761 cagtgtttat cgcaaaccac taaaaggaaa acctgatgtg ctataataaa tttatgagta
     2821 caggatggat agaagtgata tgcggcagta tgttcagtgg aaaaagtgag gagcttataa
     2881 gaagggtaaa aagggctgaa atagcccgac agaaggttca agtctttaaa ccggctatag
     2941 atacccgata tgaggacaga gaggttgtct ctcacgacgg gaataaagta tctgcttata
     3001 tggtagaaaa agctgaggat atcattgcgg aattattacc agatgtgcag gtgatagcta
     3061 tagatgaagg acagttcttt gacaatagta tagtagaggt gtgtcagact ctggcagata
     3121 acggtaagag agtaatagta gccggattag accaggattt cagaggtgaa ccgtttggtc
     3181 ctatgcctga gctacttgca gtggcagaaa aagtggataa acttcaggct atatgtatgg
     3241 tctgtggagg tccggcaagc aggactcaaa ggcttataaa tggagaacca gcgtactatg
     3301 atgatccaac tatacttatt ggggcagagg atgtctatga ggccaggtgt agagagcatc
     3361 atgttgtaag aaggaggaaa taagatgcaa atcggtggac aggcagttat agagggtata
     3421 atgatgagag gtccaaatag tttcagtgta gctgtgagga ctcaaaataa tgaagtgaag
     3481 gttcataaat ccagtacaaa tagtatattg aacagcaaaa taaataagat tccaatttta
     3541 aggggtttta tttcacttat agatagcctg gtacttggaa taaaggctct caatatatct
     3601 gcagaagaat atggtggcga agaggtgaaa ctttctaatc tggatatctt tatagctatg
     3661 gctatgggga tagggttggg tatacttctc tttatagtct ttcctgcatt tatagcgaga
     3721 atcatacaga atagcatatc ttcagccctg gtggtgaata tattggaagg gttggtaagg
     3781 ataggtgtat ttcttgtata tatctattca ttttctttta taagagatat aagaagggta
     3841 tttgaatatc atggggcaga gcataaggtc atattcactt atgaaaaggg agaacctctt
     3901 acagtagaaa atgctgaaaa gaatagcaga ctgcatccaa gatgtggaac aaatttctta
     3961 ctcatagtta tggttgtaag tatactggta ttttctctgc ttggaaaaca gaccctactg
     4021 tggagaataa tatcaagaat aattctgatc ccggtagttg ctggtatatc ttatgagctg
     4081 atacgactgg cgtccaaata tagcaactca aagattgcta aaattattac agctccagga
     4141 ctggctcttc agtatctcac aacacgggag ccgtctcagg atcaaattga ggtggctatc
     4201 gaagcggtca agtctatact ggcactggaa ggtgttcctt atgtgggata aattagaaga
     4261 gatagagaga agatatatgg agttagaaga gtcactggca aaacctgatg tgattgccaa
     4321 tcaagagtta ttccagagtc ttgcaaagac gtataacgaa ctgggagatg ttgtcggtat
     4381 ctacaggaga tataaacaga tagataagga aatctctgat atcaagtctc tcctggatga
     4441 cccggagctt ggtggtatgg cagaagaaga gttagaaaag cttgagggag agaaagaaaa
     4501 gctggaagaa gaattacgta ttaaactact tccaaaagac tatagagatg agagaaacgt
     4561 tataatggag ataagagcag gaactggtgg agaagaggca gctttatttg ccggagacct
     4621 cttaagaatg tatctgcgat atgtagagcg aaaaggatgg aaagctgaga taatgagcag
     4681 taaccccact gacctgggag gatacaaaga ggttatcgta tcaatccagg gaagaggtgt
     4741 ttatagcagg ctaaagttcg aaagtggtgt ccacagggtc cagagggtac ctgtaactga
     4801 atctggagga aggatacaca catctactgc tacagtggct atactgccag aagctacaga
     4861 tgtcgaagtg gagataaatc ctgatgaact gaagatagat acattcagag ctggcggaca
     4921 tggtggacag aatgtcaata aagttgagag cgcagtaaga ataacacata tcccaacagg
     4981 gatagttgta acctgtcagg atgaaaggtc tcagtatcaa aatagagaga aagcactgag
     5041 gatactgagg gctcatctgc tggaaatcaa agagagagaa agagagaatg aagaggctca
     5101 gctcaggaag agtcaggtgg gttctggtga acgcagtgaa aagataagaa cttataactt
     5161 ccctcaaaat agagtaaccg atcatagaat agggttaact ctatataacc ttcaggatgt
     5221 cctggacgga gctctggacg aactcataga tgcacttata agagaatatc agatagaaca
     5281 gttaaataat ctatctctga ccagatgaag ctgatagatg cgtacagaga aaccatcaga
     5341 ttattccggg ataagatacc aaactttcaa cttgaagctc tccttatact ttcgctggtt
     5401 ctggataagc ccaaagagta tatcttagcc catcaagaat cagagttatc tgaagcagaa
     5461 gtagaaaaga taagagaact tatagataaa agattatcag gtatcccgta tgcgtatctt
     5521 aaaaaagaga aggaattttt tggaatcagg ttctctgtcg aagaaggggt tttaatacca
     5581 agaccagaga cagaaactct ggtagaaata gttttaaaaa tgggaccatt cactaaaggg
     5641 ttagaccttt tctgtggaag tggtattata gggctaacta ttctatacag taatggatgt
     5701 aaagaattca cagggataga tatgtctcaa aaatgcatag atgtatctat tgcaaatgcc
     5761 agcaggttag gtttaataaa caggaccagg ttcattatgg ctgatataag aagtttatct
     5821 ttggaagagg agtttgacct gatagttgca aatccacctt acatccctga cgatagatgg
     5881 aacgaactgg atagagaagt aaagagtgaa ccgagagagg cgctcctggg aggaaaagag
     5941 gggttagact tctatcctga gatagcaaag gtcatatcta aaaatttagt caggggagga
     6001 gtatttgcag tagagatagg tggaaaagat caggtcgata a
//
LOCUS       DAOTKU010000036         6003 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_12117_length_6003_cov_3.660726, whole genome shotgun sequence.
ACCESSION   DAOTKU010000036 DAOTKU010000000
VERSION     DAOTKU010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6003
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12117_length_6003_cov_3.660726"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            151..1014
                     /gene="rmuC"
                     /locus_tag="P5310_01565"
     CDS             151..1014
                     /gene="rmuC"
                     /locus_tag="P5310_01565"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HRU32085.1"
                     /translation="MIEVLLVLLVILLCIVILYLVRREKPTSGEDIGAIKVQAENILR
                     TLQDTYTKIETQRLIDEEIQKATKRIESALLGSKSKGEIGENIIYESLRQFPPEMIDI
                     NFKVNGKPVEYAIVLSDGKRVPIDSKWVGLEAEDSPDVDIEKVISKKAKEVSQYIDPV
                     STIPMAIAAVPDSVFARCKQAHIKAYRENRVLIMPYSMTVPYILALYSLHRSYVRNID
                     TERIHSYLSEVTVSINRLESLLENNIARSVTMINNAYSEGKQILYKIRGAIDYIENSN
                     GMEEKNGEKTL"
     gene            1031..1393
                     /locus_tag="P5310_01570"
     CDS             1031..1393
                     /locus_tag="P5310_01570"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HRU32086.1"
                     /translation="MIAGVCGGVAEYLNIDPAIVRLIMALLVFADGLGIILYILALII
                     IPQNPEQSQGGVDQTEAAVHTGAQNPVTIGIILIIIGLVAILWTWGIPWLSIIRPGIF
                     LGILIIIAGIYFLIKGRE"
     gene            1397..2479
                     /locus_tag="P5310_01575"
     CDS             1397..2479
                     /locus_tag="P5310_01575"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRU32087.1"
                     /translation="MHDVIVIGAGPSGASSALFLARKGIDVLIIEKKKLPRYKLCAGG
                     TSKVIFDILDVPPDFFNAFSIEKLRVVYRGEEKEYSVPPNNIFTLRREEFDYKLTMLA
                     CQHGASLVDGEKVTGIEEDKRTITLETDLGHRFKTRYLIGADGVGSKVARFIGSNFKY
                     PVPLAIQKDIPYENPDPTISLFMGLVPMGYGWIFPKDGMVSAGLGAYKFPPREMSKVL
                     SSILNNFNHKNIPLLAHPISFYRGKIRLARGNVSLVGDSAHITDPFSGEGIRNGIKSA
                     KVVEEVILEAIKKNKTLESYTEKLYSLFARELFLSWIMSGVFYKFQNRIFSLMDRFNM
                     GYVLANLLNERTSYSELFKKIIKSII"
     gene            2587..3180
                     /locus_tag="P5310_01580"
     CDS             2587..3180
                     /locus_tag="P5310_01580"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRU32088.1"
                     /translation="MKKYREENDTELIRRIQKGDEKAFEEIIRRYSNRIINLATQIMG
                     SQDEGWDIAQEVFISVWKNIKSFDISKNFYPWVRKITVNACYEELRRRRGHIESSLDD
                     VEEGEPSIDVPDDAYSPEDIFDKIELKEAVEEALNSLPKHYRVTLWLRIIENLSYEEI
                     AETLEINIGTVKSRINMARKMMQDKLKYYLEEKDESS"
     gene            3167..3574
                     /locus_tag="P5310_01585"
     CDS             3167..3574
                     /locus_tag="P5310_01585"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HRU32089.1"
                     /translation="MSHLSFEELSQFIDKELPNWRVAEIKEHIDNCPACKEKLDTLLL
                     IDQSIRETYKEYNTEKFSAEVLERVESKPVRYSFRLKLATIGVAISLAFGITFGFLRS
                     TYRENLEEEKYKLISQHYIISSGDMGIIFISDK"
     gene            3571..4479
                     /locus_tag="P5310_01590"
     CDS             3571..4479
                     /locus_tag="P5310_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32090.1"
                     /translation="MKKIIFLPVLLILLGLIDVGTGSLIGDYISGIEPEFDYSAVAKV
                     TVGKTSDKIDILKIGEYMLLTVRGKSSSIYIVKKGETYSVVTDQPVNIGGLLWSYRFS
                     KDVKLISKNYNIVFEGYEMVNKIKSSRMTFNPKYNSGIKRTVWIGVSPKIILKLEDRD
                     RKGNLLRSREILSISLNPPSSKKKVVESLFKKGIKLPAREKLLSLHQANKELDFELVI
                     PGDLPLGYEFIGADIPQEGVGQLVFSDGIGFISIYEIKIPWWARQDMPPEKKEYIEWE
                     NKGIHFIVVGDPPTDILLSIANSMKK"
     gene            complement(4508..4750)
                     /locus_tag="P5310_01595"
     CDS             complement(4508..4750)
                     /locus_tag="P5310_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HRU32091.1"
                     /translation="MAFKITEDCIECGACIPVCPNEAITEGSPYVIDPEKCTECVPVY
                     DTQQCASVCAVDAPQPDPDHVETREQLEAKYKRLHP"
     gene            4947..>6003
                     /locus_tag="P5310_01600"
     CDS             4947..>6003
                     /locus_tag="P5310_01600"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HRU32092.1"
                     /translation="MQREYLSFYRRLRTEILQSQDEGKDLSSLEEEIRGVLEEKDNDK
                     REKLSKELLDKLQSLPPEGDFKYVEPSNLEDILEESNGEYKLYKAEIEADEKLFNKVY
                     GAWLGRIAGCLLGKPVEGWQRDKIWGFLKETDNFPLSRYMSSDMPQEIKERYGIVDER
                     GRGWINLIDHMVEDDDTNYTILDLYIFEKYGPDFSSEDVAESWLYNLPIFHTYTAERV
                     AYQNLVNLIMPPKSGEYRNPYREWIGAQIRADFWGYINPGNPLRAGEIAFRDACVSHV
                     KNGIYGEMFIAACLAWAFVSDDIEEIIKAGLSIIPKDSRFYEAVNKILGFKKEGKTFE
                     EIRDYIYENYEKDKRHEE"
BASE COUNT         2102 a          927 c         1337 g         1637 t
ORIGIN      
        1 ttataataat ctcgcacctc ttttctttac gcgtcaccca ttacccatca cgcgttacgt
       61 atccataccc gacaatatca cagaacgtta acatatcatt gaccttgaat gattaatagt
      121 gtagaatata aacgaattca aggaggcaag atgatagagg tccttttagt tctactggtc
      181 atcttattat gtatagttat actgtatctt gtcagaagag aaaaacccac atctggagag
      241 gatattgggg caataaaggt acaggcagaa aatatactga ggacacttca agatacttac
      301 actaaaatag agacacaaag acttatagac gaagagatcc aaaaagctac aaagaggata
      361 gaatctgccc ttttggggag taagtctaag ggggagatag gtgagaatat aatctatgaa
      421 tctttgagac agtttccacc agagatgata gatataaact tcaaggtaaa tggaaaaccg
      481 gtcgagtatg caatagtcct gagtgatgga aagagggttc caatagactc aaaatgggtt
      541 gggctcgagg ctgaagattc tccggatgta gatatagaaa aagttatatc aaagaaagcg
      601 aaagaggttt ctcagtatat agatccagta tccactatac ctatggctat agctgcagta
      661 ccggatagcg tcttcgcaag atgtaagcag gctcatataa aagcctatag agagaacaga
      721 gtacttatta tgccatacag tatgacggtt ccatatatac ttgcccttta cagtctccac
      781 aggagttatg tgagaaatat agatacagaa aggattcact cttatctgag tgaggtaacg
      841 gtctcaataa atagattaga aagtctgctg gaaaataata ttgccagatc agttaccatg
      901 ataaacaacg cctatagcga ggggaaacag atattataca aaatcagggg ggctatagat
      961 tatatagaaa attccaatgg aatggaggag aaaaatggag agaagactct atagatcaag
     1021 aaagaacaga atgattgcag gggtatgtgg cggagtagcc gaatatctta atatagaccc
     1081 tgcaatagta agacttataa tggcattatt agtctttgcc gatggcttag ggattattct
     1141 ctatatattg gcacttatta ttatccctca gaatccagag cagagtcaag gcggagttga
     1201 tcaaacagag gctgcagttc atacgggagc ccaaaaccca gtaacaattg gaattattct
     1261 cattataatt gggctagttg caatattatg gacatgggga attccgtggc tatctataat
     1321 aagaccagga atattcttgg gaatactgat tataattgct gggatttact tcctgataaa
     1381 agggcgggaa taatttgtgc atgatgtgat agttattgga gctggtccca gtggagctag
     1441 ctctgctcta tttttagcca gaaaagggat agatgtttta atcatagaga agaagaaact
     1501 accgaggtat aaactctgtg cgggaggaac ttcaaaggtc atttttgata tcctagatgt
     1561 tccccctgat ttttttaatg cattcagtat agaaaaactc agagtagtct atagaggaga
     1621 agagaaagag tacagtgtgc cccctaacaa tatattcacc ctccgcagag aagagtttga
     1681 ctataaactt acaatgttag cctgccaaca tggggcatcg cttgtggatg gagagaaagt
     1741 tacaggcata gaagaggata agaggactat aacattagag acagatctag ggcacagatt
     1801 taagaccagg tatcttatag gtgcagatgg cgttggttct aaggtggcaa gattcattgg
     1861 aagtaatttt aagtacccag tgccgttagc tatacagaaa gatatacctt atgagaaccc
     1921 tgacccgact atctccctgt ttatgggatt ggttcctatg gggtatggat ggatatttcc
     1981 taaggatggg atggtatctg caggattggg tgcgtataaa tttccaccca gggagatgtc
     2041 aaaagttcta tctagtatcc ttaataactt taaccataaa aatatcccat tattagccca
     2101 tccaatttca ttctacagag gcaaaataag gcttgccaga ggtaacgtat cactggttgg
     2161 tgattctgca catataacag atccgttcag cggagaaggg ataaggaatg ggataaagag
     2221 tgcaaaagtc gtagaagaag ttatccttga agcgataaaa aagaataaaa ccttagaatc
     2281 ttatacagag aaactctaca gcttatttgc cagagaactc tttttatcgt ggattatgag
     2341 tggagtattt tataagtttc aaaacaggat attttctcta atggatagat tcaatatggg
     2401 atatgtactg gctaatctgc tgaatgaaag gacaagttat tctgaattat ttaaaaagat
     2461 aattaaatct attatataga taaggaatcg aacctaatag aagatatatg catatcttta
     2521 aatataaaag aataacttta tacatccctt cctgttataa tatttctgaa gagaggatgt
     2581 ataaagttga aaaagtatag agaggaaaat gatacagagc tcataagacg catccaaaag
     2641 ggcgatgaga aggctttcga agagataata aggcggtact caaaccgtat aataaacttg
     2701 gctactcaga taatgggctc ccaggatgaa ggttgggata tagcacagga ggtctttatt
     2761 tctgtatgga agaatataaa aagttttgat atcagcaaga acttttatcc ctgggtaagg
     2821 aagattaccg ttaatgcctg ttatgaggag ctgagaagaa ggaggggaca tatagagtca
     2881 tctttagatg atgtagaaga gggtgagccc agcattgatg tgccggatga tgcatattca
     2941 ccagaagaca tatttgataa aatagagctg aaagaggcgg tggaagaagc attaaactcg
     3001 cttccaaaac actatagagt tactctctgg ttaaggatta tagaaaactt atcttatgag
     3061 gagatagcag agacattaga gataaatatt ggtactgtaa aatcaaggat aaatatggca
     3121 agaaagatga tgcaagacaa gctaaaatat tatttggagg aaaaggatga gtcatcttag
     3181 ttttgaagaa ctttcgcagt ttatagataa agagcttccc aattggaggg tggctgagat
     3241 aaaggaacat attgataatt gccctgcctg taaagaaaag ctagatactc ttttgcttat
     3301 agaccagagt ataagggaga catataaaga gtacaatacc gagaaattca gcgcagaggt
     3361 tctggagaga gtagaatcga aacctgtgag gtatagcttc aggctgaagc ttgccactat
     3421 tggtgtagct atatcattag cattcggtat tacatttggg ttcctaagaa gtacatacag
     3481 agaaaactta gaagaagaga agtataaact tataagccag cattatataa tatcttcagg
     3541 cgatatggga attatattta tatcggacaa atgaaaaaga taatctttct accagtttta
     3601 cttatactgt taggactcat agatgtaggc actggaagtc ttataggtga ctatatatca
     3661 ggtatagaac ctgaatttga ctattcagcc gtagctaaag ttaccgtagg aaaaacctct
     3721 gataagatag atattctaaa aattggagaa tatatgctgc ttactgtaag aggcaaaagt
     3781 tccagcattt atatagtaaa aaagggagag acatactcag tagtcaccga tcaaccagtg
     3841 aatattggcg gattattatg gtcatacaga ttttcaaagg atgtaaaatt gataagtaaa
     3901 aattataata tagtatttga aggctatgag atggtaaata aaataaaatc gtcaagaatg
     3961 acatttaacc caaaatataa cagcggaata aagagaactg tctggattgg agtatccccc
     4021 aaaataatcc tgaagcttga agacagggat cggaagggaa atcttctcag atcaagagag
     4081 atattgagta tctctttaaa tccgccatca agtaagaaaa aggttgtgga atctctattt
     4141 aaaaaaggga taaaattacc ggcaagagag aagcttctct cccttcacca ggctaataaa
     4201 gagttagatt ttgaacttgt cataccaggt gatttgccat tgggatacga atttattgga
     4261 gcagacatcc ctcaggaggg tgttgggcag ctggtgtttt cagatggaat aggctttata
     4321 tccatatatg agatcaaaat accttggtgg gcaagacagg atatgccccc agaaaaaaaa
     4381 gaatacatag agtgggaaaa taaaggtatt catttcatag ttgtgggaga tcctcccact
     4441 gacatccttt tatcgatagc aaacagtatg aaaaaataaa taggagggga acccctccta
     4501 taagttttta tggatgtaat cttttatatt ttgcctcaag ctgttccctt gtctcaacat
     4561 gatcagggtc aggctgaggt gcatctactg cacagacgct ggcacattgc tgtgtatcat
     4621 atacagggac acactctgtg catttttcag gatcaataac ataaggcgaa ccttctgtta
     4681 tagcctcgtt agggcataca ggtatacatg ccccacactc aatacaatcc tcggtaatct
     4741 taaatgccaa taaaatcacc tccaaaactt aaataaatct aaaacagaaa tattatacaa
     4801 cttctacgca caaaagcaaa tatgtgtatg agcttaccag aaattaccca aaaacttctc
     4861 agtctatagt agttgatttt tatcgactat gtgttataat tgcttttggg caatccattg
     4921 ctcaaaatga gactgaggga ggaaaaatgc aaagagaata tttaagtttc tacagaagac
     4981 ttagaacaga gattttgcag agtcaggacg aagggaaaga tttgtctagt ttagaagaag
     5041 agataagggg tgtcttagag gaaaaggata atgataaaag ggagaagctc tctaaagaat
     5101 tactggacaa actgcaatct cttccaccag agggagattt taagtatgta gaaccttcta
     5161 atcttgaaga tatcttggaa gaatctaatg gcgaatataa attatacaag gcagagatag
     5221 aagctgatga aaaattgttt aataaggttt atggggcttg gcttggtagg attgccgggt
     5281 gtcttttagg aaagcctgta gagggatggc agagagacaa gatctggggc tttttaaagg
     5341 agacagataa tttccccctt tccagatata tgtcttcaga tatgcctcag gagataaaag
     5401 aaaggtatgg gatagttgac gaaaggggaa gaggctggat caacctgata gatcatatgg
     5461 tagaggatga cgatacgaat tataccattc tggatttata tatctttgaa aagtatggac
     5521 cagatttttc ctctgaagat gtggcagaat cctggctcta taatcttcct atattccaca
     5581 cctatactgc agagagggtt gcatatcaga atcttgtaaa ccttataatg ccacctaaat
     5641 ctggtgaata tagaaaccca tatagagaat ggataggagc ccagataaga gcagacttct
     5701 ggggatatat aaatcccgga aatcctttaa gagcagggga gattgcattc agggatgcct
     5761 gtgtttcaca tgtaaagaat ggaatatacg gagagatgtt tattgcagcc tgtttagcct
     5821 gggcatttgt ctcagatgat atagaagaga taataaaggc aggactttcc attataccaa
     5881 aggatagcag gttctatgaa gctgtgaata agatattagg ctttaaaaaa gaagggaaaa
     5941 catttgaaga gataagagat tatatctacg agaattacga aaaagataaa cgtcatgaag
     6001 agt
//
LOCUS       DAOTKU010000037         5864 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_12422_length_5864_cov_3.776726, whole genome shotgun sequence.
ACCESSION   DAOTKU010000037 DAOTKU010000000
VERSION     DAOTKU010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5864
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12422_length_5864_cov_3.776726"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="P5310_01605"
     CDS             complement(<1..459)
                     /locus_tag="P5310_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011944099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRU32093.1"
                     /translation="MFYKKLFSLILVLFLILGIVPVVFSQGKPVRGGLYNLSDYEKLT
                     GKKISRFNEAPILAELVKQGKLPSVEKRLPEDPLVIEPVEEIGRYGGELRSAALGPAA
                     FSDIEHAREFYFFRPDSQGKGIQPNIAKKYEVSRDGRTVIVYLRKGMKWSD"
     gene            complement(616..1959)
                     /locus_tag="P5310_01610"
     CDS             complement(616..1959)
                     /locus_tag="P5310_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013429225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HRU32094.1"
                     /translation="MVFNVKDFGATGDGINKDTDAFKKAISECQKNGGGTLYIPAGIY
                     LTGPIHLKSNITLYIENGAVLKFSQDIDDYPTVYTRWEGEEAEVYSPLIYGEDIENIA
                     VTGFGTIDGQGMLWWNLLRKKELRYPRPRGISFYRSRNITIDGIRIINSPSWTVNPIE
                     CENITVNNIKIQNPYDSPNTDGINPESCRGVRISNCYIDVGDDCVTLKSGTEDCRERI
                     PCENITVTNCIMAHGHGGVVIGSEMSGGVRNVVISNCIFEGTDRGIRIKTRRGRGGIV
                     EDIRVSNIVMKNVICPFAFYMYYNCGKGGQEKRVWDKSAYPVDETTPVVRRIYVSDVT
                     VRQARAAAGFLYGLTEMPIEDVVFSNVVIDMADNPEPEIPAMISFLEPMAKRGFILNT
                     VKNARFLNVSVNRQDGTAFEFENCENIELYRCSSDGEKDYSKFISKKNVKGIITE"
     gene            2105..2893
                     /locus_tag="P5310_01615"
     CDS             2105..2893
                     /locus_tag="P5310_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013085530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HRU32095.1"
                     /translation="MAVIQMNLSSRYLMMDTSVTVIIPVLSGGNNEKTLEEVYRRDVK
                     YPTLYLLHGYSGDSTNWLRYTSIERYANEKRLAVVMPSGYNSFYTDIPYGFKCWSYIS
                     EELPLAMRAIFPLSEKREENFVAGLSMGGYGAMKWALRRPEFFSAAASLSGALDIVEI
                     VESAEPEGKRGFENVFGDPSKLRGSDNDLFYLAENLVKENKTSPRLYIACGTEDFLYP
                     ANIRFKEHLIRLGYDITYEEGRGTHNWYFWDKYIRRVIDWIFSV"
     gene            2919..5228
                     /locus_tag="P5310_01620"
     CDS             2919..5228
                     /locus_tag="P5310_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HRU32096.1"
                     /translation="MERDIKRIISHMTLEEKASLCSGLDFWHTKPVERLGIPSIMMTD
                     GPHGLRKQDETTGGGLHNSVPATCFPTASCLACSWDRELIKKVGKAIGEECQAEGVSI
                     ILGPAVNIKRSPLCGRNFEYFSEDPYLSSELSRNHIDGVQSQGVGTSIKHFAANNQEH
                     RRMTIDEIIDERTLREIYLASFESAVRFAQPWTVMAAYNKINGEYCTQNKYLLTRILK
                     EEWGHRGFVVSDWGAVDDRVMALESGLELEMPSSYGINDRKIVEAVKNGRLPEEVLDR
                     AVERLLTIIFKAIENKKEGASYDKLAHHKLAKEVARECMVLLKNEDNILPLKKEGVIA
                     IIGAFAKNPRYQGSGSSHINPTMLDIPYEEIKKVAGENVKLLYADGYSLAEDMVDENL
                     INEAKEIAERADLAIIFAGLPDEYESEGYDRAHMCMPESHNKLISDVADVQKKTVVVL
                     CNGAPVEMPWIDKVKGLFECYLAGQAGGSAIAELLFGLANPCGKLAETFPEKLSHNPS
                     YLNFPGEDDRVEYREGIFVGYRYYDKKGIEPLFPFGYGLSYTSFEYTGIFIDKKEISD
                     TETVKVTVNVKNTGNLAGKEIVQLYVKDVESSVIRPDKELKGFEKIELQPGEEKSVVF
                     TLDKRAFACYNVDINDWYVETGDFEILVGRSSKEILLREKIKVNSTARVSKKFTRNTT
                     IGDLLEDPVGAEVFRQLVEQFKQSSPIFGNVENDNPQMLMAILKYMPLRGLINFSRGV
                     FTEEMLNALLEKLSSSASAATSTAATTST"
     gene            complement(5175..>5864)
                     /locus_tag="P5310_01625"
     CDS             complement(5175..>5864)
                     /locus_tag="P5310_01625"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HRU32097.1"
                     /translation="HIKSNPLEFQKVYKQWADSVKSKVNLLGFFDDKGYRLASKISFI
                     FLLVGIAFIIIYIATFNEANSQALSLLIGAGLGLLFGGVLGVIISVIFKPGFNRRTET
                     GALHYKRWNNLKKFLTDFSQIETMPPESLILWEKYLVYGTSLGVADKVISSMKILLPE
                     VSQSSAISSYPLIRASAKGIEPLAFAKMLNNFSSVAHSSAYYSASSGHGGGFSGGGGG
                     GGGGGGGGGAG"
BASE COUNT         1801 a         1152 c         1184 g         1727 t
ORIGIN      
        1 atcagaccat ttcatcccct ttcttaaata cactataacc gttctaccat ccctggaaac
       61 ttcatatttt ttagcaatat taggctgtat cccttttcct tgagaatcag gtcggaaaaa
      121 gtagaactct ctggcatgtt caatatctga aaaggcagct ggacctaaag ctgcgcttct
      181 cagctctccc ccgtatctac caatctcttc taccggttct ataaccagcg ggtcttctgg
      241 caacctcttc tctaccgatg gaagctttcc ctgttttacc agctctgcta atattggtgc
      301 ttcattgaac ctggaaatct tcttaccggt aagcttctca taatctgaga ggttatataa
      361 tcccccacga acaggtttcc cctgtgaaaa gactacaggg acgattccca gaataagaaa
      421 taatacaaga atcaatgaaa aaagcttttt gtaaaacata tacactccct cctgtaatat
      481 aagatttttg aagctttgat caatattctc tcttaaatct tatcataact tctcaaaaat
      541 tcaacagctc atttccccaa tacctcttag aaactaaatc tcattatttc tctctgaact
      601 tttcgtaagt ttttattatt cggttattat acctttcaca ttctttttgc ttataaactt
      661 tgaataatct ttctccccat ccgaactaca cctgtagagc tctatgtttt cacaattttc
      721 aaattcaaag gctgtaccat cctgtctatt tactgataca tttaaaaatc tggcattctt
      781 taccgtattc aaaatgaatc ccctcttagc catcggctca aggaaactta tcattgccgg
      841 aatctcaggc tctggattat ctgccatatc aatgacaaca tttgaaaata cgacatcctc
      901 aattggcatc tcagtaagac cgtataaaaa tcctgcagca gctctagctt gccttactgt
      961 gacatcactt acatagattc tccttacgac aggtgttgtc tcatcaaccg gataggcaga
     1021 tttatcccat accctttttt cctgtccacc tttcccacag ttataataca tataaaaggc
     1081 gaatgggcat ataacatttt tcatcacgat attagagact cttatatctt caacaatccc
     1141 tcctcttcct ctacgtgttt ttatccttat ccctctgtcg gtgccttcaa atatgcagtt
     1201 tgagataacc acattcctta ctcctccact catttcactt ccaattacca ccccaccatg
     1261 cccatgtgcc attatacagt ttgtaacagt tatattttca cagggaattc tctctctgca
     1321 atcttctgtg ccagacttta atgtaacgca atcatcccca acatctatat aacaatttga
     1381 tattctgaca cctctacagg attctggatt tattccgtca gtattgggag aatcgtatgg
     1441 attttgtatc tttatattgt taaccgttat attttcgcat tcaattggat ttacggtcca
     1501 gctcggtgaa ttgattattc tgatgccatc aattgtgata ttccttgatc tatagaagct
     1561 tataccacgt ggacgtggat atcttaattc tttcttcctg agaagattcc accataacat
     1621 cccctgtcca tcgatagttc caaaaccagt tactgcaata ttttctatat cttctccata
     1681 aattaatggg gaataaactt ctgcctcttc accttcccat ctggtataga cagtggggta
     1741 atcatctata tcctgcgaaa actttaatac agccccattt tctatgtaaa gggttatatt
     1801 gctcttcaga tgaatgggtc cagtaagata tataccagcc ggtatatata aagtaccacc
     1861 tccattcttc tggcactctg atattgcttt tttgaatgca tcggtatctt tattaatccc
     1921 gtctcctgta gctccaaaat ccttaacatt aaaaaccacc ggcgttgtcc ctccttcaga
     1981 cctaaacaat agatagtcta attatatatc tatctacttt tctgtcaatg aagtcttaac
     2041 ttgatttttc tgtcttgcaa cccggtttat aatattagta tcaaaagaaa aaggaggaaa
     2101 aacaatggca gtcatacaga tgaacctttc atctagatat ctgatgatgg atacaagtgt
     2161 aacagttatt attccagttt tatccggggg gaacaatgag aaaaccttag aagaggtcta
     2221 caggagagat gttaaatatc caactctcta tttactccat ggttattcgg gagacagtac
     2281 caactggctc aggtatacct ccatagagag gtatgcaaat gaaaaaagat tagctgtggt
     2341 tatgccgtca ggatataata gcttttatac cgatatcccg tacggattta aatgctggtc
     2401 ttatataagt gaggaattac cactggctat gagggctatc ttcccattat ctgagaagag
     2461 agaggaaaac tttgtggcag ggttatctat gggtggttac ggtgcaatga agtgggctct
     2521 cagaagacct gaatttttca gtgcagctgc cagtctttcc ggtgccctgg atatagtcga
     2581 gatagttgaa tctgcagagc ctgagggtaa gcgtggtttt gaaaacgtct ttggagaccc
     2641 ctcgaagttg agggggagcg ataatgacct gttttatctg gctgaaaatc ttgttaaaga
     2701 gaataaaact tcgccaagat tatatatagc ctgcgggaca gaagactttt tgtatccagc
     2761 caatatcagg ttcaaggaac atctaataag attggggtat gatataactt atgaggaggg
     2821 tagaggtaca cataactggt acttctggga taaatatata aggagagtta tagactggat
     2881 attttctgtt taaatagaat aaattctgga ggtgttttat ggagagagat ataaagagaa
     2941 taatttctca tatgaccctt gaggaaaaag ccagcctttg ttcaggactt gacttctggc
     3001 atacaaaacc tgtagagaga ttgggaatcc catccattat gatgaccgat ggaccacatg
     3061 gtctgagaaa acaggatgaa acaacaggag gaggattaca taatagtgtt cctgccacct
     3121 gttttcctac cgcttcctgt ctagcctgtt cttgggatag agaacttatt aaaaaagtag
     3181 gtaaggctat aggtgaagag tgtcaggcag aaggagtttc aataatactt ggaccagctg
     3241 tcaatataaa aaggtctccc ctctgtggga gaaactttga gtatttttcg gaagacccat
     3301 acctctcgag cgaactgtcc agaaatcata tagatggggt acagagccag ggggtgggga
     3361 catctataaa acattttgcg gcaaataatc aggaacatag aaggatgact atagatgaaa
     3421 ttatagatga gagaacactg agggagatat atctggcaag ctttgaatct gctgtgaggt
     3481 ttgcacaacc ctggacggtg atggctgcat ataataagat taatggagag tattgcactc
     3541 agaataaata cctgcttacc aggatattaa aagaggaatg gggacataga ggttttgttg
     3601 tatccgactg gggagcggtt gatgataggg ttatggctct agaatcaggg ctggaactgg
     3661 agatgccatc cagttatggt ataaatgata gaaagatagt ggaagcggta aaaaatggaa
     3721 gacttccaga agaagttttg gatagagctg tagagagact gttaactata atatttaagg
     3781 ctatagagaa taaaaaagaa ggggcaagtt acgacaaact ggctcatcat aaactggcaa
     3841 aagaagttgc aagagaatgc atggtactgc tgaagaatga ggataacata ctcccattaa
     3901 agaaagaagg agtcattgct attataggtg catttgccaa aaaccccaga tatcagggga
     3961 gtggaagttc tcatataaat cctacaatgc tggatatacc atatgaagag ataaagaagg
     4021 ttgcagggga gaatgtgaaa cttttatatg cagatggtta tagcctggca gaggatatgg
     4081 tggatgaaaa cctgattaat gaagcaaaag agatagcaga aagggctgat ctggctataa
     4141 tatttgcagg cttaccagat gagtatgaat ctgaaggtta cgacagggca catatgtgta
     4201 tgcctgaaag ccataataaa cttatctctg atgttgcaga tgtccagaaa aaaactgttg
     4261 ttgtcctctg taatggtgct cctgtcgaga tgccatggat agataaggtt aaggggctat
     4321 ttgaatgtta tctggctgga caggcaggag gttctgcgat agcggagtta ttgtttggtc
     4381 ttgcaaaccc ctgtggaaaa ctggcagaga catttccaga aaaactgagt cataatcctt
     4441 cttatttaaa tttcccgggt gaggacgaca gggttgagta tagagaaggt atctttgttg
     4501 gatacagata ctacgataag aaagggatag aacccctatt cccatttggc tatggtttaa
     4561 gttataccag ctttgaatat actggaattt tcatcgataa aaaggagata agtgatactg
     4621 aaactgtaaa ggtcactgta aatgtaaaga atacaggtaa tctcgccggg aaggagatag
     4681 tccagctata cgtaaaagat gtagagagca gtgtaatcag accagataaa gaactgaagg
     4741 gttttgagaa gattgaactc caacccggag aagagaagtc tgtagtattt acacttgata
     4801 agagggcttt tgcctgctac aacgtggata taaatgactg gtatgtggag actggagatt
     4861 ttgagatact tgtgggcagg tcatctaaag agatactcct cagagaaaag ataaaggtta
     4921 actctactgc aagggtcagt aagaagttca ccagaaatac caccataggt gacctgctgg
     4981 aagaccctgt gggggctgaa gtcttcagac agttagtaga acagttcaag cagagcagcc
     5041 ctatctttgg caatgtcgaa aacgacaatc ctcagatgtt aatggcaatc ctcaaatata
     5101 tgcctctaag aggactgatt aatttcagca ggggagtttt cactgaagag atgttaaatg
     5161 ctctcctaga gaaattatcc agctccgcct ccgccgccac ctccaccgcc gccaccacct
     5221 ccacctgaga atcctccacc atgacctgaa gaggctgagt agtaagcaga ggagtgagct
     5281 acagaagaaa agttattcag cattttggca aacgctaagg gttctatccc ctttgcgctt
     5341 gcccttatta atgggtagct gcttattgca gatgactgtg aaacttcagg taagagtatc
     5401 ttcatactgg aaattacctt atctgcaacc cctaaagatg tgccatatac aagatatttt
     5461 tcccagagta tcaaggattc tggaggcata gtttctatct ggctaaagtc agttaaaaac
     5521 ttttttagat tgttccatct tttatagtgt aaggcacctg tttctgttct cctgttaaac
     5581 ccaggtttaa agattactga gattataact cccaatactc ctccaaagag caaccctaat
     5641 cctgcaccta tcaaaagact caatgcctga gaattagcct cattaaaagt agctatgtaa
     5701 attattatga aagctatccc aacgagtaga aatataaaag aaatctttga tgccaatctg
     5761 tagcccttat catcaaagaa accaaggaga tttaccttcg attttacaga atcagcccat
     5821 tgcttataaa ccttttgaaa ctccagaggg ttactcttta tatg
//
LOCUS       DAOTKU010000038         5761 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_12672_length_5761_cov_3.291798, whole genome shotgun sequence.
ACCESSION   DAOTKU010000038 DAOTKU010000000
VERSION     DAOTKU010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5761
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12672_length_5761_cov_3.291798"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1554
                     /gene="uvrA"
                     /locus_tag="P5310_01630"
     CDS             <1..1554
                     /gene="uvrA"
                     /locus_tag="P5310_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRU32098.1"
                     /translation="EIIGMVPEELIDFINNLQLTERETYIAHQLLKEIKARLQFLIDV
                     GLDYISLERPAATLSGGEAQRIRLATQIGSGLMGVLYILDEPSIGLHARDESKLIETL
                     KKLRDLGNTVIVVEHDESMMRSADYIVDIGPGAGENGGYLVASGTLEEIMRCEESITG
                     QYLSGKRSIPIPARRREPNGKYLTIVGAKEHNLKDITVNIPLGVLVLVTGVSGSGKST
                     LVNDILYKALERELHRARVTPGKYGRIIGIENVDKVINIDQSPIGRTPRSNPATYTGV
                     FDDIRKVFAETHSAKTRGYKPGRFSFNVRGGRCEACHGDGMIKIEMHFLPDVYVPCEV
                     CNGKRYNRETLEVKYKGASIADVLDMTVDQALIHFENVPAVRQKLQTLQDVGLGYIKL
                     GQPAPTLSGGEAQRVKLASELSRKSTGKTLYILDEPTTGLHFADIEKLINVLQRLVDM
                     GNTVVVIEHNLDVIKVADYIIDLGPEGGDKGGYIIAEGTPEEISKNPKSYTGMFLKRV
                     LKIEEEVYA"
     gene            1547..3292
                     /gene="uvrC"
                     /locus_tag="P5310_01635"
     CDS             1547..3292
                     /gene="uvrC"
                     /locus_tag="P5310_01635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HRU32099.1"
                     /translation="MPSLIDDIFPENPGVYIMRDKNNNVLYVGKAKVLQNRIRSYINP
                     DNPRIRDMVSQVEDVDYIVTSSEIEALVLENNLIKQYRPPYNVRLKDDKTYPYILITK
                     DKYPILTVTRRIEDKGEYFGPYTSTQALRAVIHVIRKRFKIRSCTGDPTRFKNACLNY
                     HLGLCSAPCINDSLTFHKSYMQDIKEAVKVLNGDSKEIINDLKEEMFHMAGELDFEKA
                     AVLRDQIIALKKTAEKQYVVSTKKIDADVFAIFTLKSNYSAVVLFIRRGVLIGLEDFE
                     IENPGEEDVLGAFVTAFYRNGNYIPPLVVVNQEIKDKKLLEEWLNSRSQRRVDIKYPR
                     KGELKKLLDIAEKNVLERFNQKIVSIEDPLISLKEVLGLSKLPETIEGYDISNIRGMT
                     AVGSMVVFKNGKPYKNGYRKFRIKTVEGPNDYAMLQEVLWRRLDNGDLELPDLILIDG
                     GLGQVGAAREVLASKGLDIPVIGLAKREEVIWFPEGNSLRLSRRSEALRLLQKVRDEA
                     HRFAVKYHHQLREKELQSSLLDIPGVGKERTKKILLSYPDLSTIKDIPVEELAKSCRI
                     PKNVAEEVKKWVENL"
     gene            3274..3747
                     /locus_tag="P5310_01640"
     CDS             3274..3747
                     /locus_tag="P5310_01640"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HRU32100.1"
                     /translation="MGGKSMTYSLSVPLYGVFKYKMNDKGLTDLEWLDGIKTEGDEND
                     PLGLGDLLKRYFIGERINFGDIPIDYGNINSTYRRILEIVRSIPYGKVHTYSEIGEMI
                     GKPRYARVVGNAMRINQCPIVIPCHRVIGKNGLGGYGFGIQKKFLLLKLEGVMVC"
     gene            3741..4067
                     /locus_tag="P5310_01645"
     CDS             3741..4067
                     /locus_tag="P5310_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HRU32101.1"
                     /translation="MLKRWLILTIAICLTPYIISGISVRSFVDALIAALILGILNIII
                     KPVLKILTLPINIITLGLFTLVINAILLEMVAWLIPGFSIVSFWSAILGGLFISIVSW
                     ILGWIF"
     gene            4077..4748
                     /gene="pyrF"
                     /locus_tag="P5310_01650"
     CDS             4077..4748
                     /gene="pyrF"
                     /locus_tag="P5310_01650"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001273.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HRU32102.1"
                     /translation="MNPEIIVALDITDSIRIFELIDRLGDAISYYKIGYIAFYKFGWE
                     LVDRLYSMGKKVMLDLKLDDISNTVSNAVTLVTEHNPSFLTIHSSIGRDALTKAQEAA
                     RGKTNLLGITLLTSLDKEDLKDLSIYGEVEDIVLKRAKLCKEAGLSGVVASAREAALI
                     KEELGKEFIVVTPGIRLSSGGDDQKRTASYREARERGVDFVVIGRPIYNASDPLKVLE
                     MMEEI"
     gene            4745..5158
                     /locus_tag="P5310_01655"
     CDS             4745..5158
                     /locus_tag="P5310_01655"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRU32103.1"
                     /translation="MKEISSGCIVYKIENNDVYLLMILDRFGKWTFPKGKIEEGESLE
                     ETAIRELEEETGIKVKIEQYIRETNYSYTDENRGTIEKTVYWYLGRAESEEINPRYGE
                     IKEAKWVQIDFATELCGYDSDRDTVREVYKILSDR"
     gene            5155..>5360
                     /locus_tag="P5310_01660"
     CDS             5155..>5360
                     /locus_tag="P5310_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32104.1"
                     /translation="MTVEIIRLISTLLPLIWLGYALISRIVRPHIACIISLLITGIAS
                     ITVWRISADILLWSILDGVIFSLFP"
     assembly_gap    5361..5370
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5371..>5761
                     /locus_tag="P5310_01665"
     CDS             <5371..>5761
                     /locus_tag="P5310_01665"
                     /inference="COORDINATES: protein motif:HMM:NF014683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HRU32105.1"
                     /translation="TIFTALFTYRLVCETGAIEGIKRSLSGISNNRLIQAFIVAFCFG
                     SLLEGIAGFGTSVIIPSAILLSLGFPPYQSAMISLISNTVPVVYAAVGVPVIALAKAT
                     DLPLKALSGTIGLQLFPVTLIIPFLIK"
BASE COUNT         1976 a          925 c         1356 g         1494 t
ORIGIN      
        1 gagattatcg gtatggttcc tgaggaactt atagacttta taaacaatct tcaacttaca
       61 gagagagaaa cctatatagc ccatcaacta cttaaggaga taaaggcaag actccaattc
      121 ttgatagatg taggattaga ctacatatct cttgagcgtc cagccgccac attatcaggt
      181 ggggaagctc aaaggataag gcttgcaacc cagataggtt caggtcttat gggcgttctg
      241 tatatcctgg atgaaccaag tatagggctt catgcaagag atgagtctaa acttatagaa
      301 acattaaaga aactgagaga tttagggaat actgtaatag tagtagaaca cgatgaatct
      361 atgatgagat cagctgatta tatagtagac ataggaccag gagctggaga aaatggtgga
      421 tatctggtag catctggaac attggaagag attatgcgat gtgaagaatc aataactggt
      481 cagtatcttt ccggtaagag aagtattcct ataccagccc gtagaaggga accaaatggt
      541 aaatacctca cgatagttgg agctaaggaa cacaatctga aggatataac cgtaaatatt
      601 cctttaggag tattggtcct ggtaacaggt gtctctggtt ctggaaagag tacgctggta
      661 aatgatatac tctacaaagc attggagaga gaactccaca gggcaagggt gactccgggc
      721 aaatatggca ggataattgg aattgaaaat gtagataaag tgataaatat agatcagtct
      781 cccataggta gaacacccag atcaaatccc gctacctaca ctggagtatt tgatgatata
      841 agaaaagtct ttgcagagac gcattctgca aagaccaggg gttataaacc cgggagattt
      901 agctttaatg taaggggagg aagatgtgag gcatgccacg gggatgggat gataaagata
      961 gagatgcact ttctcccgga tgtatatgtc ccctgtgagg tgtgtaatgg caagaggtat
     1021 aacagagaga cattagaggt aaagtataag ggggcaagta tcgcagatgt gcttgatatg
     1081 acagtagacc aggcattgat acatttcgaa aatgtcccgg cggtaaggca gaaacttcag
     1141 acactgcagg atgtagggct tgggtatata aagctgggtc agccagcacc caccctatct
     1201 ggtggggagg ctcaaagggt aaaactggcc tcagaactct ccagaaaaag taccggtaaa
     1261 accctgtaca tacttgatga accaacaaca gggttacact ttgccgatat agagaagctt
     1321 ataaatgtgc tgcaaagact tgtagatatg ggaaacacgg tggtagtgat agaacataac
     1381 ttagatgtaa taaaagtagc agattatata atagacctgg gacctgaagg tggagataaa
     1441 ggtggctata ttatagcaga gggaacacct gaagagatat ctaaaaaccc taaatcttat
     1501 accggaatgt ttctaaaaag ggtactgaag atagaggaag aggtttatgc ctagtctaat
     1561 agatgatata tttcctgaga atccaggcgt atatataatg agagataaaa acaataatgt
     1621 gctgtatgtt gggaaagcaa aggtcctgca gaatagaatc aggtcatata taaatccaga
     1681 caatccaagg atacgagata tggtatctca ggtagaagat gtagattata ttgtcacatc
     1741 ttcggagatt gaagccttag tgttagaaaa taatctaata aaacagtata gacctcctta
     1801 taatgtaaga cttaaagacg ataaaactta cccgtatatc ctgattacta aagacaaata
     1861 tccaatcctc actgtgacac ggaggataga ggataaaggg gaatacttcg gtccttatac
     1921 aagtacccag gcgctcagag cggttataca tgtgatacgt aagagattta agataagaag
     1981 ctgtacaggg gatcctacca gatttaagaa tgcctgcctg aactatcatc tgggactgtg
     2041 ttctgctccc tgtataaacg atagcctaac attccacaaa tcatatatgc aagatataaa
     2101 agaagctgta aaagtgttaa atggagatag caaagagata attaacgact tgaaagaaga
     2161 gatgttccat atggcaggag agttagattt tgagaaggct gcagtcttga gagatcagat
     2221 aatagccctg aaaaagacag cagaaaaaca atatgtggta agtaccaaaa agatagatgc
     2281 agatgtattt gccattttca ctcttaaatc aaattacagt gcagtagtac tattcataag
     2341 aagaggggta cttataggac tggaagattt tgagattgaa aatcctggtg aagaggatgt
     2401 actgggggca ttcgttactg cattttatag aaatggaaac tatatcccgc cacttgtagt
     2461 tgtcaatcag gagataaagg ataaaaaact cctggaagag tggctgaact caaggtctca
     2521 gaggagggta gacataaaat atccaagaaa gggagagctt aaaaagcttt tagacatagc
     2581 agagaaaaac gttttggaga gatttaatca aaagatagtt tctatagaag accctcttat
     2641 aagcctgaaa gaggtactcg gattatccaa acttccagag acaatagaag gctatgacat
     2701 ctccaatata agaggaatga ctgcagttgg ttctatggtt gtatttaaaa acggtaaacc
     2761 atacaaaaat ggctacagaa agttcaggat aaaaacggta gaaggaccta acgactatgc
     2821 aatgctgcag gaggtcctct ggagaaggtt agacaatggt gacttagaat tacctgatct
     2881 catactgata gacggtggac tgggacaggt tggagcagca agggaagttc tggcatcaaa
     2941 agggctagat ataccggtga taggactggc aaagagggaa gaggtaatct ggtttcctga
     3001 gggtaacagc cttagacttt ccagaaggtc cgaggcttta agattactgc agaaggtaag
     3061 ggatgaggct catagatttg ctgtaaaata tcaccaccag ctaagagaaa aggagttgca
     3121 atcaagtctc ctggatatac ctggcgtagg taaagaaagg acgaaaaaga tactgttgtc
     3181 ttatcctgat ctatcaacca taaaggatat acctgtagaa gaactagcta agagttgtcg
     3241 catccctaaa aatgtggcag aagaggtaaa aaaatgggtg gaaaatctat gacatactcg
     3301 ttgagtgtac cgctatacgg tgtatttaaa tacaaaatga atgataaagg tttaacagat
     3361 ctagaatggc tggatgggat aaaaactgaa ggggatgaaa atgacccttt gggtctggga
     3421 gatctgctta agagatattt tattggtgaa aggataaatt tcggtgatat acccatagac
     3481 tacggtaata taaattctac ctacaggaga atactggaaa tagtgaggag cataccttat
     3541 ggaaaggttc atacctattc cgagatagga gagatgattg gaaaacctag atatgcaaga
     3601 gtggttggta atgcaatgag gattaatcaa tgtccgatag taataccctg ccacagagta
     3661 ataggtaaaa atggactggg aggctatggg tttggaatcc agaagaaatt cttactatta
     3721 aaactggaag gagtaatggt atgttaaaac gttggcttat acttacgata gcaatatgtc
     3781 tcacacccta tatcatctcc gggatatcag taaggagctt tgtagatgct cttatagctg
     3841 ctttaatctt aggcattcta aatataataa taaaacctgt acttaagata ctaacattac
     3901 ccataaacat aattacattg ggtttattta cgctggtgat aaatgctatc ttgctggaga
     3961 tggtagcctg gcttatcccc ggattttcta tagtatcttt ctggtctgcc atattggggg
     4021 gactctttat aagtatagtt tcctggatac tgggatggat attctagggg atagcgatga
     4081 atcctgagat aatagtagcc ttagacatta cagatagtat cagaatattc gaacttatag
     4141 acagattggg agatgctatc tcatactata agataggtta tatagccttc tataaatttg
     4201 gctgggaatt agttgataga ctttacagta tggggaaaaa agtgatgtta gacttaaaac
     4261 tggacgatat atccaatacc gtatccaatg ctgtaacatt agtcacagaa cataaccctt
     4321 ccttcctgac aatacacagc agtataggta gagatgcctt aactaaagca caggaagcgg
     4381 ctaggggaaa gacaaattta ctgggtataa ctctactgac cagtctggac aaggaagatc
     4441 tgaaagattt aagtatatat ggagaggttg aagacatagt cctgaaaaga gctaaactct
     4501 gtaaagaggc gggtctatcc ggtgtagtgg caagtgccag agaggctgcc cttattaaag
     4561 aagagctggg gaaagaattt attgttgtaa ctcctgggat aaggctatct tcaggcggag
     4621 atgatcagaa gagaaccgca tcttatagag aggccagaga aagaggggta gactttgttg
     4681 ttataggtag acccatatac aatgcaagtg acccattaaa agtattagag atgatggagg
     4741 agatatgaaa gagatatctt ctggatgtat agtctataag atagaaaata atgatgtcta
     4801 tctacttatg attttggacc gatttggtaa atggacattt ccaaagggaa agatagaaga
     4861 aggtgaatct ctggaagaaa ccgctataag ggaattagag gaagagaccg ggataaaagt
     4921 aaagatagag caatatataa gagagacaaa ctacagttac acagatgaga atagaggaac
     4981 tatagaaaaa acagtctact ggtatctggg aagagccgaa tccgaagaga taaaccccag
     5041 gtatggtgag ataaaagagg caaagtgggt acagatagac tttgccacag aactctgtgg
     5101 ttatgattca gacagggaca ctgtaagaga ggtatacaaa atcttatcag acagatgact
     5161 gtcgaaataa taagattaat ctctactctt ctacctctga tatggcttgg ttatgccctt
     5221 atctccagaa tcgtaagacc gcatatagca tgtattatat ctctattaat aaccggtata
     5281 gcttctataa ccgtctggag gatatcagct gatatcctcc tctggagtat tttagacgga
     5341 gttatatttt ctctattccc nnnnnnnnnn ggacaatatt caccgctctt tttacctata
     5401 gactggtatg tgaaacaggg gctatagagg ggataaagag aagtctatca ggtatttcca
     5461 acaacagact gatacaggct tttatagtag cattttgttt tggctctctc cttgaaggga
     5521 tagccggttt tgggacatca gttataattc cgtcagcgat attgctatca ttaggtttcc
     5581 ctccatatca atcagccatg atatctctga tatctaatac tgtcccggta gtatatgcag
     5641 ccgtaggtgt tccggtaatc gccctggcaa aagctacaga tcttccatta aaagcactca
     5701 gcgggaccat aggattacag ctctttccag taacattaat aataccattt cttataaaaa
     5761 g
//
LOCUS       DAOTKU010000039         5750 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_12695_length_5750_cov_3.143810, whole genome shotgun sequence.
ACCESSION   DAOTKU010000039 DAOTKU010000000
VERSION     DAOTKU010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5750
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12695_length_5750_cov_3.143810"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="P5310_01670"
     CDS             <1..202
                     /locus_tag="P5310_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HRU32106.1"
                     /translation="IRINFVGAIFLAALAISPSFIRSSQISAFTSVFFGSGVLIVVGV
                     AIETVRQLESQLIMRHYEGFLK"
     gene            214..879
                     /locus_tag="P5310_01675"
     CDS             214..879
                     /locus_tag="P5310_01675"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HRU32107.1"
                     /translation="MTIYGVLLGPPGVGKGTQAKMIAERFDLAHISTGDILRDAVNKR
                     TPLGLKVADIMKSGGLVSDEIINSLMEEYIKGLPDGFLLDGYPRTLGQATFLDNFLKD
                     IGKGLNIALAIDVQDDEIIRRLTGRRYCPKCGRGYHIIYDPPKEDGVCDNDGEKLVQR
                     EDDKPETIRERLRVYRERTAPLIDYYKNRGLLEVVDGSGSIEEVHRLVLQVLERRGLV
                     DKA"
     gene            866..1618
                     /gene="map"
                     /locus_tag="P5310_01680"
     CDS             866..1618
                     /gene="map"
                     /locus_tag="P5310_01680"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HRU32108.1"
                     /translation="MIRRKTQREIDVMFLAGQIVAKVLKVLQEMVVPGISTFDLDQKA
                     EELIRNLGARPAFKGYNGFPGSICISINEEVIHGIPKKSVKIKEGDIVKIDIGAEYNG
                     YYADSATTIPVGKVSPEVENLLEVGRKALEIGISNAKPGNHLSDISHSIESFVLAHNM
                     SVVKSFVGHGIGRELHEEPQVPNYGPPGTGPILEPGIALAIEPMVNLGKEDVVILKDG
                     WTVITRDKKPSCHFEHTVIVQDGEPLITTRLK"
     gene            1656..1874
                     /gene="infA"
                     /locus_tag="P5310_01685"
     CDS             1656..1874
                     /gene="infA"
                     /locus_tag="P5310_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HRU32109.1"
                     /translation="MPEKKDVIEVEGIVVEALPNAMFRVELDNGYRVLAHISGKIRLN
                     FIRILPGDRVKIELSPYDLTRGRIVRRL"
     gene            1879..1992
                     /gene="rpmJ"
                     /locus_tag="P5310_01690"
     CDS             1879..1992
                     /gene="rpmJ"
                     /locus_tag="P5310_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006042225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HRU32110.1"
                     /translation="MKVRASVKKMCSKCSIIRRNGKLMVICSNPKHKQRQG"
     gene            1996..2367
                     /gene="rpsM"
                     /locus_tag="P5310_01695"
     CDS             1996..2367
                     /gene="rpsM"
                     /locus_tag="P5310_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HRU32111.1"
                     /translation="MARIAGVDLPRNKRIEVALTYIYGIGFPTSKKILQEAGVDPDKK
                     ANDLTEEEIARLREIIENNYRVEGDLRREVSQNIRRLIDLGTYRGLRHRYGLPVRGQR
                     TRTNARTRKGPRRTVGAHSKK"
     gene            2381..2767
                     /gene="rpsK"
                     /locus_tag="P5310_01700"
     CDS             2381..2767
                     /gene="rpsK"
                     /locus_tag="P5310_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HRU32112.1"
                     /translation="MAKRRTTRRREKKGIVKGIAHIHSTFNNTIVTITDPNGSVVCWA
                     SAGTCGFKGTKKGTPFAAQLAAETAAKKAIDQGMRQIDVYVKGPGPGREAAIRSLQAA
                     GLDITLIKDVTPIPHNGCRPPKRRRV"
     gene            2768..3394
                     /gene="rpsD"
                     /locus_tag="P5310_01705"
     CDS             2768..3394
                     /gene="rpsD"
                     /locus_tag="P5310_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HRU32113.1"
                     /translation="MSRYTEAKCRLCRREGAKLFLKGEKCSSVKCILEKRKTAPGQHG
                     ITRVRFSEYRLRLREKQKARRIYGISETQFRNYFELASKRKGVTGETLLQLLERRLDN
                     VLYRLGFAASRSQARQLIAHGHVLVNGKKLDIPSYLVKPGDRIALKGKIMSGDLGETL
                     KERVTEVRLPSWLEVNSDQLEGMVRSLPSRQEIDIPVNEQLIVEFYSR"
     gene            3413..4381
                     /locus_tag="P5310_01710"
     CDS             3413..4381
                     /locus_tag="P5310_01710"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HRU32114.1"
                     /translation="MFHVEWLKHEKNYGQLYIEPLERGYGHTVGNALRRVLLSSIEGC
                     APIGVRIDGVLHEFSTIPGVKEDVVDILLNLKGLVVRLHNSELKTISIEKRGPKIITA
                     EDFIPDSDVEIINPDLYIATLDNEDSVLRMDVVIQRGKGYVSADKLKGTFTSVGYIPM
                     DAFFSPVKQVSYKVENTRVGQITDFERVVLDIWTNGAITPAESLLSASRILKDSYDNL
                     TRLLESELQHLIFREDLAAHIPEETPVEISPVTQESGTPIESLGLSLRIVNLLKRGGI
                     HTVESLIEKTPKEIKSIKNIGDKMLLEIEEKLRNAGYILKQEEGVK"
     gene            4384..4731
                     /gene="rplQ"
                     /locus_tag="P5310_01715"
     CDS             4384..4731
                     /gene="rplQ"
                     /locus_tag="P5310_01715"
                     /inference="COORDINATES: protein motif:HMM:NF013369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HRU32115.1"
                     /translation="MRHRKKGRRLGRFSSEKNALMRNLLTSLFKYESVITTEAKAKEL
                     IPVAEKVISIARKQDINAIRRVSGIIYEKEVASKIFSSTDLFRKESGHFHVYKLGVRQ
                     GDGAPLVKIALIK"
     gene            4731..5540
                     /locus_tag="P5310_01720"
     CDS             4731..5540
                     /locus_tag="P5310_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013382255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HRU32116.1"
                     /translation="MSIEVKNLSFDYEGISVLRDVNFQIDRGEFIGIAGPNGSGKSTL
                     ARCLNGIFSPVKGEVVVDGISVKDTSKQWIVRQTVGLVFQNPDNQLVASIVEEDVAFG
                     PENLGIPPAIIRERVQYALERVGLINERKRPVSSLSGGQKQRVAIAGILAMRPEYIVL
                     DEPTSMLDPVGRKEVLSTVLQLKEEGVGIIYITHHLEELVYADKIILLYKGEVFKEGS
                     AREILTDEETLTRIGLDIPEIPALSLRLKGLGIDFNETPLTIEEMVNVLFN"
     gene            5524..>5750
                     /locus_tag="P5310_01725"
     CDS             5524..>5750
                     /locus_tag="P5310_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011917284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRU32117.1"
                     /translation="MSSLIEMNDVWYQYNPDSPLAYWALMGVNLRIERGEFISIIGPT
                     GSGKSTLIQHFNGLLKPAKGEVIVDGVNISRT"
BASE COUNT         1842 a          937 c         1491 g         1480 t
ORIGIN      
        1 tatcaggata aatttcgtcg gtgcaatatt ccttgccgca cttgccatat caccatcctt
       61 tatccgttct tctcagataa gtgcttttac atctgtattc ttcggttctg gtgtattgat
      121 agtagtaggg gtagctattg agacggtaag acaacttgag tctcagctta ttatgagaca
      181 ctatgaggga ttcttaaaat agaggtgata gatatgacca tctatggcgt actgctaggt
      241 cctccggggg ttggaaaggg aacccaggct aaaatgatag ctgaaagatt tgatcttgca
      301 catatatcta caggagatat cctcagagat gcggtaaata aaaggactcc actgggttta
      361 aaggttgctg acataatgaa gagtggtgga ttggtttctg atgaaattat aaattctctt
      421 atggaagagt atataaaggg acttcctgat ggtttccttc ttgatggata tcccaggact
      481 cttgggcagg ctacttttct cgataatttt ttaaaagata tcggtaaggg attaaacata
      541 gccctggcta ttgatgttca ggatgatgag attattagaa ggcttactgg caggagatac
      601 tgtccgaaat gtggtagggg atatcatatc atctatgatc ctcccaaaga ggatggggta
      661 tgtgataatg atggagagaa actcgtccag agggaagatg ataaaccaga aacaataaga
      721 gaaagacttc gagtttacag ggaaagaact gcccctctta tagactacta taaaaataga
      781 ggcttattag aggtggttga tggttcaggt tcaatagaag aggtccacag attggttctt
      841 caggtcctgg aaaggagagg tctggttgat aaggcgtaag actcagagag agatagatgt
      901 aatgttttta gcaggtcaga tagtggcgaa ggttcttaag gtacttcagg aaatggttgt
      961 cccgggtata tctacttttg atcttgatca gaaagccgaa gaacttatca gaaatctcgg
     1021 tgccagacct gctttcaagg gttataatgg gtttccagga agtatctgta tatcaataaa
     1081 tgaagaggtc atacatggga taccaaaaaa gtcagtcaag ataaaagagg gggatatagt
     1141 aaagatagac attggagctg agtataatgg atactatgct gactctgcca ccaccatacc
     1201 ggtggggaag gtatcgccag aggtggagaa tctactggaa gtaggaagga aggctctgga
     1261 aataggtata tctaatgcga aacctggtaa ccatctatct gatatatcac atagcataga
     1321 gtctttcgtt ctggctcata atatgagtgt ggtaaagagt tttgttgggc atgggatagg
     1381 tagagagttg catgaagagc cgcaggtgcc gaattatgga cctccgggga ctggaccgat
     1441 acttgagccc ggaattgccc tggctataga gccaatggtg aatctgggga aagaagatgt
     1501 ggtgattctt aaagatggct ggacagtgat aacgagggat aagaaaccgt catgtcattt
     1561 tgagcatact gtcattgttc aggatggtga acctctaatc actactagac taaagtaaat
     1621 gaatctggta tactttaata attgggtggt gtaaattgcc agaaaaaaaa gatgttattg
     1681 aagttgaggg tattgtagtg gaggctctac cgaatgcgat gttcagggta gagcttgata
     1741 atgggtatag ggttcttgcc catatctccg ggaagatcag gttaaacttt atccgtattc
     1801 tgcctggaga tagagttaag atagaacttt ctccatatga ccttacccgt ggacgtattg
     1861 taaggaggtt ataaaagaat gaaggtacgc gcttcagtta agaagatgtg tagtaagtgc
     1921 agtattataa gaaggaatgg taagcttatg gtaatctgtt caaatccaaa acataaacag
     1981 aggcagggat agtatatggc tagaatagcg ggagtagact tacccagaaa taagaggata
     2041 gaggtagccc ttacatatat atatgggata ggttttccta ccagtaaaaa gatcctgcag
     2101 gaagctggtg tggaccctga taaaaaggca aatgacctca cagaagaaga gattgccaga
     2161 ctcagagaga ttatagagaa caactacagg gttgaaggtg accttagaag agaggtctct
     2221 cagaatataa ggagacttat agaccttgga acttacaggg gattaagaca cagatatggg
     2281 ctacctgtac ggggacagag aaccaggaca aatgcaagaa caaggaaagg tccacgtaga
     2341 actgtagggg ctcatagtaa gaagtgagat aggaggtatt atggctaaaa gaagaacaac
     2401 aagaagaaga gaaaagaaag gtatagtaaa aggtatagca catatacact ctacttttaa
     2461 caatacgata gttaccatca ctgaccccaa tggctctgtg gtatgctggg caagtgccgg
     2521 gacatgtggg ttcaagggaa ctaaaaaggg aaccccattt gccgctcaac tggcagcaga
     2581 gacagctgct aagaaagcca ttgatcaggg tatgaggcag atagatgtat atgttaaagg
     2641 acctggacct ggaagagaag ctgctataag gtctctgcag gcagcaggtc tggatataac
     2701 tctgataaaa gacgtcacac caatacctca taatggatgt cgtccaccta aacggaggag
     2761 agtgtaaatg agtagatata cagaagcaaa atgcagatta tgtagaagag aaggggcaaa
     2821 actctttctt aagggggaga aatgttcctc tgttaagtgt atcctggaaa agagaaaaac
     2881 tgccccagga cagcatggaa tcacaagggt cagattttca gagtacagac tgagattgag
     2941 ggaaaaacag aaggctcgcc gtatatacgg tatttccgag acacagttca gaaattattt
     3001 tgagcttgcc tcaaagagga aaggtgttac cggtgagaca ttgcttcagc tactggagag
     3061 aaggctggac aatgtgctct acagactggg atttgctgca tccagaagtc aggcgagaca
     3121 gctcatagct catgggcatg tcctggtgaa cgggaaaaag ctggatatac catcatacct
     3181 ggtaaaacca ggagacagga tagccctgaa agggaagatt atgtccggag atttaggaga
     3241 aacactgaag gagagagtaa cagaggtaag acttccatcc tggcttgaag ttaattcaga
     3301 tcagctggaa ggaatggtga gaagtctgcc aagcagacag gagatagata tccccgttaa
     3361 tgaacaactt atagtagagt tctactctag ataaaaaggg ggggattatt gaatgtttca
     3421 tgtagaatgg ctcaagcacg aaaagaatta tggacaactt tatatagaac cgcttgaaag
     3481 aggttatgga cataccgttg gaaatgcatt gagaagagtg ctcctctctt ctatagaggg
     3541 ctgtgcccct ataggagtaa gaatagatgg ggtattgcat gagttttcta ctatcccagg
     3601 ggttaaagaa gatgttgtag atatactcct taatttaaaa gggctggttg tgagactgca
     3661 taacagtgaa cttaagacaa tctccataga gaagagaggt ccaaagataa ttaccgctga
     3721 agattttatt ccagatagtg acgttgagat tataaaccct gacctctata tagctacact
     3781 ggacaatgag gactctgtat tgaggatgga tgtggttata cagagaggaa agggatatgt
     3841 atcggctgac aaacttaaag ggacatttac atctgttggt tatatcccaa tggatgcatt
     3901 tttcagtccg gtaaaacagg tgagctataa ggttgaaaac accagggttg gacagatcac
     3961 tgacttcgaa agggtggtcc tggatatctg gacaaatgga gcgataacac cggcagagtc
     4021 tttattgagc gcttccagga tattaaagga tagctatgac aatctgacca gacttctaga
     4081 gagtgagctt cagcatctta tcttcagaga agacctggct gcccatatcc ctgaagagac
     4141 tcccgtggag atatcacctg ttacccagga atcagggacc ccaatagaat ctctggggct
     4201 ttctctgagg attgtaaacc tgctgaaaag agggggaatt catacggtag agtctcttat
     4261 agagaagaca cccaaagaaa taaaaagtat aaaaaatata ggggataaga tgcttttaga
     4321 gatagaggaa aaacttcgaa acgctggata catcctaaag caggaagaag gggtgaaata
     4381 aagatgcgtc atcgtaaaaa gggtagaaga ttagggcgtt tttcttctga gaagaatgca
     4441 ttaatgagaa acctgttaac gtctctattc aaatatgaaa gtgttattac cactgaagca
     4501 aaggcaaaag aacttattcc tgtagctgaa aaggtaattt ctattgccag gaaacaggat
     4561 attaacgcta taagaagagt atctggaatt atatatgaaa aggaagtggc aagtaagatt
     4621 ttttcttcta ccgatctctt tagaaaagag agcggacatt ttcatgtcta caagttaggg
     4681 gtaagacagg gggatggagc tcccctggta aaaattgccc ttataaaata atgtcaattg
     4741 aggtaaaaaa cctctccttt gactatgagg gaatttcagt actgagagat gtaaattttc
     4801 agattgacag aggggaattt ataggcatag cgggtccaaa tggctccgga aagtctaccc
     4861 ttgccagatg tcttaatgga attttctcac cggtcaaagg ggaggttgtg gttgatggaa
     4921 tatcagtaaa agatacatcg aagcagtgga ttgtcagaca gactgttggt ttagtatttc
     4981 agaatccaga taatcaactt gtggcaagca tagttgaaga ggacgtggct tttggtcctg
     5041 aaaacctggg tatacctcct gctataataa gggaaagagt tcagtatgca ctggagagag
     5101 tgggtcttat caacgagagg aagagaccgg tttccagtct ttccggtggt cagaaacagc
     5161 gagttgccat agccggtata ctggctatga gacccgaata tattgttctg gatgagccca
     5221 cttcaatgct ggaccctgtt ggcagaaaag aagtgttatc aactgtgctc cagctgaagg
     5281 aagagggggt aggaatcatt tacataacac atcatctgga agaacttgtc tatgcagata
     5341 agataatact gctctataag ggtgaagtat ttaaggaggg ttctgccaga gaaatcctta
     5401 ccgacgaaga gaccctgacc aggataggtc ttgatattcc tgagattcct gccctgtctt
     5461 taaggctaaa gggattgggg atagacttca atgaaacgcc gttgactatt gaggagatgg
     5521 taaatgtcct ctttaattga aatgaatgat gtctggtatc agtataatcc ggattctcct
     5581 cttgcctact gggctttgat gggtgtcaat ctgaggattg agagagggga gtttatatct
     5641 attataggac caactggttc tggcaagtcc acccttatac agcatttcaa tggattgttg
     5701 aagcctgcaa agggagaagt tatagtggat ggggtgaata tctccaggac
//
LOCUS       DAOTKU010000040         5705 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_12804_length_5705_cov_2.410088, whole genome shotgun sequence.
ACCESSION   DAOTKU010000040 DAOTKU010000000
VERSION     DAOTKU010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5705
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12804_length_5705_cov_2.410088"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..681)
                     /gene="tsaD"
                     /locus_tag="P5310_01730"
     CDS             complement(<1..681)
                     /gene="tsaD"
                     /locus_tag="P5310_01730"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HRU32118.1"
                     /translation="MYILGIDTSCDDTSVAILKDGYEVISNLVSSQNDIHQRFGGVVP
                     ELASRRHLENITPMIKLSLEEAKIDKEDISVIGVTYGPGLVGSLLVGLMVAKGLAYSL
                     KKPLIGINHMEGHIFGAILEHRDLYPPFIALVVSGGHTDLVLVRRYFSYEIIGSTRDD
                     AAGEAFDKVARLLDIGYPGGPAIERFARLGSIDYNLPRAIPEGYDFSFSGLKTAVIRL
                     LKGKENIDL"
     gene            complement(650..1138)
                     /gene="rimI"
                     /locus_tag="P5310_01735"
     CDS             complement(650..1138)
                     /gene="rimI"
                     /locus_tag="P5310_01735"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HRU32119.1"
                     /translation="MMIEIKKMTVKHLKEVLRIEELSFPSPWSFQSFYNEIVSNPYGY
                     YIVAVEDNRVVGYGGMWILFNECHITTIAVDPTRRREGIGRKIMEHLIDKAKEQDVDW
                     ISLEVRISNTSAQNLYKSLGFEAIGVRRGYYQPNNEDAIIMRLTLKGRRYNVYPGYRH
                     IL"
     gene            complement(1132..1758)
                     /gene="tsaB"
                     /locus_tag="P5310_01740"
     CDS             complement(1132..1758)
                     /gene="tsaB"
                     /locus_tag="P5310_01740"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HRU32120.1"
                     /translation="MVSLAIDTSSSYLSIAIVKDDITLYEKNVYSLKLHSVLLPGEID
                     RALQITKEKIDRIFVAIGPGSFTGLRVGLSLVKGIALGSGLPVTGVPTFDGLAYNIPQ
                     GDSLIIFMRYRMNMAYYGKYSSINNRWICERTGVSDIKNLTKLVNGEMVAVVKEHNYR
                     EIADMFSNPVLMVPRASLLVIPGKDMEPENPENLVPLYIEPSEAEKIL"
     gene            complement(1739..2218)
                     /gene="tsaE"
                     /locus_tag="P5310_01745"
     CDS             complement(1739..2218)
                     /gene="tsaE"
                     /locus_tag="P5310_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010191771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HRU32121.1"
                     /translation="MNENCFIFYSISPTETEVFGETIGRLSGGGELLLLEGTLGAGKS
                     VLVKGIAKGLDIKNEIVSPTFIIIRSYKGRLILNHIDLYRIDSIDGLGLEEYLDDEDS
                     ITVIEWGEKLLNHLPLDEYLLIRIDVVDENSREIVLEPRGERYIEYTRRIKDGISGY"
     gene            2297..3523
                     /locus_tag="P5310_01750"
     CDS             2297..3523
                     /locus_tag="P5310_01750"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRU32122.1"
                     /translation="MSKRVVVIGGGPAGMMAGGTAGSRGLDVILIEKNDTLGKKLLLT
                     GKGRCNFTNDTDINGFINNIPVNGKFLYSAFSKFSNRDLIKFFNDLGVKTKVERGGRV
                     FPASDRAEDILNALKRYLRENNVKIARGEVKKILADKQKVLGVILKDGSYISSASVII
                     ATGGLSYPDTGSTGDGYRFARELGHSIVTPKPSLVPLETEESWVSKLQGLTLKNISIR
                     VIDSNNSIIYQDLGEMLFTHYGISGPVILSASSHMRDMDKNKYRIIIDLKPGLDDEEL
                     DRRIQRDFSRYSRRYFSNALDDLLPKSIIPVIVELSGIPYDKYVNQISREERKGLVEL
                     LKGLTVTVSRFRPVREAIVTSGGVRVNEINPSTMESKIVKGLFFAGEVIDVDGYTGGF
                     NLQIAFSTGYVAGQNC"
     gene            3624..4859
                     /locus_tag="P5310_01755"
     CDS             3624..4859
                     /locus_tag="P5310_01755"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRU32123.1"
                     /translation="MLKALGDWDLLPVYAKIAEALKEEIRNGHFRPGDKLPTEEELCK
                     IYSVSRGTVRRAFQILESEGTISKRQGIGSFVKNGSEKISAKSIGLILPYISYMAADL
                     LLGIEKAIRSFGYHLVFFSSEGNVDLETGIINKLINSDISGIILYTLEGEYNDEGIRR
                     LIASEKPFVLIDRYLPHISTSWIVSDNYNGGYKMTEYLIGKGHRKIGFAIYSEEYYEV
                     TSIRDRKLGYLNAMERYELSPVIIDSYPPMPPQNEEMLRESFNELMEKIVKNIREENL
                     TAIFGINDMTAVRIMRSLLDRGYSVPDDVSVVGFDNMRILRDVGIHLTSIYQDFFNMG
                     FEAGKLIIEKIEDNSNLEDKQISIPVEIREGNTVKSPVKEVMRTREVKDSVMFGAVRG
                     IERGKRKVLRFKKLLYEEV"
     gene            4862..>5705
                     /locus_tag="P5310_01760"
     CDS             4862..>5705
                     /locus_tag="P5310_01760"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRU32124.1"
                     /translation="MFGKKILVGLLSVVLVLSSLTLAFGKTTLTFWHTITGEPYKSSI
                     QHIVDEFNKQNPDIEVKATVVASSAVTDVSKLLTAVAGGVGPDVYYVDRFTVAQRAVA
                     GVLEPLDSLTTADEIAQLKKAYLPFALDEASWDGKIWALPFDTDTRALYYRRDHFREV
                     GLSGPPKTIAELDTYAEKLTKKDPSGRFLRIGFVPWLSQGWHYTWGWAFGGEFYNPKT
                     KKLTITDPNIVKSFEWQKSYADKYGIAAIQSFSSAFGSEAQDPFIMGMISMKVDGDWT
                     LSGLT"
BASE COUNT         1715 a         1091 c         1209 g         1690 t
ORIGIN      
        1 aaggtcaatg ttctcctttc ccttcaatag tcttatcaca gcggtcttca atccgctgaa
       61 actgaaatca tagccttccg gtatagccct ggggaggttg taatctatac tgccaagcct
      121 ggcaaatctc tctatggctg gtcctccagg atagcctata tcgagtagcc ttgccacctt
      181 gtcgaacgcc tctcctgctg cgtcatccct ggttgaacct ataatctcat aagaaaaata
      241 tcttcttaca agtaccaggt ctgtatgtcc tcctgagact accagcgcaa taaagggagg
      301 atacaggtct ctatgctcaa gtatcgctcc gaatatatgt ccttccatat gatttatacc
      361 gataaggggc ttcttcagag aataggcaag tcccttggca accataagcc caaccaggag
      421 tgaacctaca agccctggac cataagttac ccctataaca gagatatcct ctttatcaat
      481 cttagcctct tccagagaaa gttttatcat aggggttata ttctctaggt gtcttcttga
      541 tgcaagctca gggaccactc caccaaatct ctgatgtata tcattttgtg agctcaccag
      601 atttgatatg acttcatatc catccttcag gatggctaca gaggtatcat cacaggatgt
      661 gtctataccc aggatataca ttataccttc ttccctttaa tgttaatctc ataataatgg
      721 catcctcatt gttaggctga tagtatcccc gtcttacgcc tattgcctca aatcctagtg
      781 atttatacag attttgagca gaggtatttg agattctgac ctctaaggat atccagtcta
      841 catcctgttc ctttgcctta tctatgagat gctccattat cttcctgcca atcccttctc
      901 ttctccttgt cggatccact gctattgtgg ttatgtggca ttcattaaaa agtatccaca
      961 tcccaccata acctacaacc ctgttatcct ctacagccac tatatagtag ccataaggat
     1021 tactaactat ttcattataa aaagactgaa aactccatgg agatggaaat gataactctt
     1081 ctatccttaa aacctcttta aggtgtttca ccgtcatctt ttttatctca atcataatat
     1141 cttctcagcc tcagaaggtt ctatatataa gggaaccaga ttctctggat tttctggttc
     1201 catatcttta ccaggtataa ctaaaaggct tgccctgggc accatcagga ctggattgga
     1261 aaacatatca gctatctccc tgtagttgtg ttcttttacc actgcaacca tttcaccgtt
     1321 aaccaatttg gtcagatttt ttatatctga aacacctgtt ctttcacata tccacctgtt
     1381 atttatggaa ctgtatttcc catagtaagc catattcatc ctgtatctca taaagatgat
     1441 gaggctatct ccttggggta tattataggc aagtccgtcg aatgttggaa cccctgttac
     1501 aggtagacca cttccgaggg ctataccctt tacaagggag agtccaaccc tcagacctgt
     1561 aaaggaccct ggaccaatag ccacaaatat tctatctatc ttctcttttg ttatctgtaa
     1621 agccctgtct atctcgcctg gcaagaggac agagtgtaac tttaaagaat agacattctt
     1681 ctcataaagg gttatatcat cctttactat agctatggac aggtagctgg aactggtatc
     1741 aatagccaga gataccatct tttattctcc ttgtatactc aatatatctc tctcctcttg
     1801 gttccaaaac aatctccctg gaattttcat ccaccacgtc tattcttatg agcagatact
     1861 catccagagg taaatggtta agcagctttt ctccccattc aataacggta atgctgtctt
     1921 catcatccag gtactcttct aatcccagcc catctatact gtctattcta taaaggtcta
     1981 tatggttaag tataagcctg cctttatagg accttataat tataaatgtc ggactgacta
     2041 tctcattctt tatatccagt cctttagcta tcccctttac caggacactc ttacctgctc
     2101 caagagtccc ttcaaggagg aggagttctc cacccccgct taacctgcca attgtttcac
     2161 caaaaacttc agtctctgtg ggagaaatag agtaaaatat aaaacagttc tcattcatag
     2221 atgatatttt atcacaagat accagaaatt tgataatatt atatctatct gaaattgtta
     2281 ccaggaggtg tataaaatat ctaaaagagt agtagttata ggtggaggtc cagctggaat
     2341 gatggctgga ggtaccgccg gaagtagagg acttgatgta atcttaatag agaagaatga
     2401 taccctgggc aagaaactct tgctgacggg aaaaggaagg tgtaacttta ccaacgatac
     2461 agatataaat ggttttatta ataatatacc tgtgaatggt aaattcctct acagtgcctt
     2521 cagtaaattt tcaaatagag acctcataaa attcttcaat gatcttgggg taaagaccaa
     2581 agtagaaagg ggtggaaggg ttttccctgc ctcagatagg gcggaagata tactgaatgc
     2641 gctgaagagg tatttaagag aaaataatgt aaagatagct aggggagagg taaagaagat
     2701 attggctgat aaacagaaag ttctgggggt tatccttaaa gatggaagtt atatctcctc
     2761 tgccagtgta atcatagcta ccggtgggtt atcctatccc gatacgggtt ctacaggaga
     2821 tggatacaga tttgcaaggg aattggggca cagtatcgtt actccaaagc catcactggt
     2881 tcctttagag accgaagagt cctgggtttc aaaacttcag gggttaactc ttaaaaacat
     2941 ctccataaga gtgattgata gtaataacag tataatttat caagaccttg gggagatgct
     3001 ctttacacat tatgggatct ctggaccggt aatactgtct gccagttcgc atatgagaga
     3061 tatggataag aataaataca gaatcatcat agacctgaag cctgggctgg atgatgagga
     3121 gctggataga agaatccaga gggatttcag caggtattcc aggagatact tttccaatgc
     3181 tctcgatgac ctgttaccca aaagtatcat acctgtaata gtggagctgt ctggtatccc
     3241 ttatgataag tatgtaaacc agatatccag agaggagagg aaggggcttg tagagctact
     3301 gaaaggtctt accgtaacag tctcacgatt cagacctgtg agagaggcta ttgtcacatc
     3361 tggaggggta agggttaatg agatcaatcc atccactatg gagtcaaaga tagtaaaggg
     3421 gcttttcttc gccggagaag taattgatgt cgatggctat actggaggat tcaatcttca
     3481 gatagcattc tcaaccggat atgtggctgg acagaactgt tgatgagttg ttcttcccga
     3541 atctggattg acaagtcgaa ggattttatg atatattact atcaggttgt atatataata
     3601 tacatctggt atagagaggg gatatgttaa aggctttggg tgattgggac ctgttacctg
     3661 tatatgccaa gatagcggag gctctgaagg aagagataag gaatggtcat tttagacctg
     3721 gagacaagct tcctaccgaa gaagaactct gtaagattta tagtgtaagt cgcggtactg
     3781 taagaagagc ttttcagata ttagagagcg agggtactat ttctaaaagg caaggtatag
     3841 gtagttttgt aaagaacgga tccgaaaaaa tttctgcaaa gagtatcggt ttaatcctgc
     3901 catatatctc atatatggca gcggacctcc ttcttggtat agaaaaagca atcaggtctt
     3961 tcgggtatca tcttgtcttc ttcagttccg agggtaatgt ggacctggaa acaggtataa
     4021 taaataagtt aataaattca gacatcagtg gaatcattct ctatacgtta gagggagaat
     4081 ataacgacga gggaataaga agattaatag catctgaaaa gccatttgtc cttatagata
     4141 gatacctccc ccatatcagt acatcctgga ttgtgtcaga caattataac ggtggctata
     4201 agatgactga atatttgata gggaaagggc accgtaagat aggatttgcc atttattctg
     4261 aagaatatta tgaggttacc tctataaggg atagaaaact gggctattta aacgctatgg
     4321 agagatatga actctctcct gtaattattg attcttatcc tccaatgcca cctcagaatg
     4381 aagagatgct aagagaaagc tttaatgagc ttatggagaa gatagtgaag aatattcggg
     4441 aggaaaacct taccgctata ttcggtataa acgatatgac tgctgtaagg ataatgagat
     4501 ctctgctgga tagaggatac agtgtgcctg acgatgtatc tgtagtaggg tttgataata
     4561 tgaggatact cagagatgtt ggaatccatc ttacatctat ttatcaggat ttctttaata
     4621 tgggctttga agccggtaag cttataatag agaagataga ggataattct aacctggaag
     4681 ataagcaaat ctctatacct gtagagataa gagaagggaa tactgtaaaa tctcctgtaa
     4741 aggaggtgat gcgaaccaga gaagtaaaag attcagtgat gtttggtgca gtcaggggga
     4801 tagaaagagg taaaagaaag gttttaagat tcaaaaaact attatacgag gaggtttaga
     4861 gatgtttggt aagaagattt tagtagggtt attatcggta gttttagtct taagcagctt
     4921 aacacttgca tttggaaaga ctactctaac tttttggcac accattaccg gagagccgta
     4981 taaatcttca atacagcata ttgtagatga gtttaataag caaaatccag atattgaagt
     5041 aaaggctact gttgtagcat cgagtgctgt aacagatgta tccaagcttt taactgctgt
     5101 agctggtggt gtaggccctg atgtatatta tgtagatcgt tttaccgttg ctcaaagagc
     5161 agtggctggc gttctagagc cattagatag cttaactact gcagatgaga tagctcaact
     5221 taaaaaagcg tatttgcctt ttgctctgga tgaagctagc tgggacggga aaatatgggc
     5281 attaccattt gatacagata caagagccct gtattataga agggaccact ttagagaagt
     5341 agggctcagt ggaccaccca aaaccatagc agagctggat acttatgctg agaaattaac
     5401 taagaaagat ccaagtggtc gattcttaag gataggtttt gttccatggt tatctcaagg
     5461 ctggcattat acctggggat gggcttttgg tggagaattc tataatccaa agacaaagaa
     5521 actcaccatc acagatccaa atattgtaaa gtcctttgaa tggcagaaga gttatgcaga
     5581 taagtatggt atagctgcta tccagtcttt ctcttccgct tttggttctg aagcacagga
     5641 cccattcatt atggggatga tctctatgaa ggttgatgga gactggacgc tttctggatt
     5701 aacga
//
LOCUS       DAOTKU010000041         5665 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_12899_length_5665_cov_3.297683, whole genome shotgun sequence.
ACCESSION   DAOTKU010000041 DAOTKU010000000
VERSION     DAOTKU010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5665
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12899_length_5665_cov_3.297683"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..830)
                     /locus_tag="P5310_01765"
     CDS             complement(<1..830)
                     /locus_tag="P5310_01765"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HRU32125.1"
                     /translation="MEEIIRKLKEKSIGGDVFFRKGISTSVTFEANRLKSIESSESSG
                     AGLRVIVDNKIGYASQYGVYEADWLLEKAIATANIDTGLKLPSGLKVQEESYNEAIDK
                     LSVEDLTELGKVMIESILKSYPEVICNVEISKDKGETLIRNTEGVECLRRRASLGLGV
                     HVNRIEGTDMLDIYEGDAVKELDKLDKEKLLSKVFTALEASKEIVKPSRTQIPVIFTP
                     KAVYVLLLPIISALSGKTILMGASPLQDKLDKKIFDEKFTFIDDPFDFDGLSGTSFDD
                     "
     gene            complement(832..2208)
                     /locus_tag="P5310_01770"
     CDS             complement(832..2208)
                     /locus_tag="P5310_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015169522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HRU32126.1"
                     /translation="MEEIKEIFLKLAKEKRIRYGELRLEESEVTNISFRGKELEELSV
                     SKSVGGNIRILGRCGWGFISFNDINTEAITDYFEEACNASNLITGEEIEIEYSDPVVD
                     RVVFPMGRDFRDIPIEEKKRLLSEYNDIMLNFSPKIQSTVVRYGDRFNKRYFINTEGT
                     DIVQEKGDISSVLIALAREEDLIEQYHYSTGSIEGFQIMENLHDKARDTAEMAVRLLS
                     APVVKGGQYTVILDPRLAGVFIHEAFGHLSEADHVYENERLKELMLIGKRFGPDFLNV
                     VDDPTYPNLRGSYRYDDEGILSRKTYLIREGILSGRLHSRETARKMGEPVTGNARSIN
                     YRFQPIVRMSNTFIVPGSSSFDELISDIKHGIYAVGAYGGQTSMEMFTFSAQEGYMIE
                     NGKIGDMVRDVVLTGNVFTTLQNVEAIGNDLQFSQGGGCGKGGQSPLPVATGSPHIRI
                     RNVVIGGR"
     gene            complement(2247..4556)
                     /locus_tag="P5310_01775"
     CDS             complement(2247..4556)
                     /locus_tag="P5310_01775"
                     /inference="COORDINATES: protein motif:HMM:TIGR01069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HRU32127.1"
                     /translation="MDERSLRLCEFDFVLGKLSSLTETPMGKDIALRLSPSSSKEEIL
                     RNSAETEEAKRLISYEPSPSLSGLVDVSPLVKKASLGVILEPQELLHIAEFISRLPSV
                     KRYIRKSALFKELADNIPELSSLEDDIRRAIDEDGNIKPNASPKLAGIRRGIARLEES
                     VRSYLESHIGREWRDFVQESYITIRNGRYVIPIKSSQKRAIKSIVHDVSQSGSTLFVE
                     PYEMVDMNNNLQELKKEEEYEIQRILGELSLRVSSSGEELISARDAISRIDFIIAKAR
                     LSLSWNCIAPVWIDKPMVRFKSARHPILGNRAVPVDVEVGESFDILVISGPNTGGKTV
                     TLKTVGLLIMMAQAGLQLPVDLAEMGIFKNVFVEIGDEQNIAQDLSSFSAHLTHLIPF
                     IRYADRNTMVLIDEPVTGTSPREGSALAISILEALRDNGARVIVASHYDELKIWASKQ
                     DRVMNGAMEFDPQTFLPTYKLRIGRPGVSNAFVIARKLGLNESIIRRAEKLLSTDELS
                     LTRLLDQVHQLELELRSELEEAKRERSYYEELKDRYEKLLDELSAQQKIILRRSKEEA
                     RELVVQTKIRLDELYDRAKSSSGRREITSIKDEIKSLEPVPEEPEIQGDRITTPRTGM
                     TVYVPSLKQNGEIIDINESKVTVLIGKLKVDVSKDELYTPVMVEKEQPQSDGSLKIKV
                     DKSRTVSNRITLRKLTVEEALDTLSKYLDDALLAGVSPIYIIHGKGEGVLRRAVTNFL
                     KEHPMVESFRLGEYNEGGWGVTVAYLKGL"
     gene            4610..5554
                     /locus_tag="P5310_01780"
     CDS             4610..5554
                     /locus_tag="P5310_01780"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HRU32128.1"
                     /translation="MPETLIINFNPALDRTAVVDRYNPYGVNKAKELIVLPGGKGVNL
                     GRALKTLGYTDFVCSGIIGGNIGRLYNALLDREGIPNDFFWIRDETRIAYATYEESTG
                     VGVITNEQGPNVTEEEITGFNNFIITKYTTANIIILSGGSPPGVPPEDIRGLVNIFKN
                     KDKQIFIDTSGDILRLCADTGPYCLKINEDELKDAFGVDLEAKDALREFYSSLARLGT
                     KWLIVTRGENGAVFIVDNKVLSGKNKRIYSHYAIGSGDAFLAGILYGSLKRFSPEEIL
                     TLAMSCGCANTLHFGACVFDREDCERIKNEIIIEESYL"
BASE COUNT         1585 a         1370 c          933 g         1777 t
ORIGIN      
        1 tcatcatcga aagatgtccc tgataagcca tcaaagtcaa atgggtcatc tataaaagta
       61 aacttctcat cgaagatttt cttatccagt ttatcctgaa gaggtgaagc acccatcagt
      121 atggtctttc cacttaaagc agatatgatc ggaagtaaaa gcacataaac agccttgggg
      181 gtaaagatca ccgggatttg agtcctggat ggcttaacta tctctttact ggcttctaaa
      241 gcagtgaata ctttagaaag gagcttttct ttatccagct tatcaagctc tttcactgca
      301 tctccttcat atatatccag catatccgtt ccttctatcc tgtttacatg cacccccaac
      361 cccagggaag cccttcttct caggcactct acaccttcag tattccttat taaagtctca
      421 cccttatcct tactgatttc aacgttacat ataacctcag ggtaagactt gagaatactc
      481 tctatcatta ctttacctaa ttctgtcaga tcttcaacag aaagcttatc tatcgcctca
      541 ttgtaggatt cttcctgtac ctttaaccct gacggaagtt tcaatcctgt atctatatta
      601 gccgtggcaa tcgccttttc tagcagccaa tcagcctcgt atactccata ttgactggca
      661 taacctatct tattgtccac tatgaccctc aagccagccc cggagctttc gctagactct
      721 atagacttca acctgttagc ctcaaaagtc accgatgtgg agataccttt tctgaaaaat
      781 acatcccccc caatagactt ttctttaagt ttcctgataa tctcttccat attatctccc
      841 ccctatgacg acatttctta ttctgatatg aggacttcca gtagcaacag gtagagggga
      901 ctgacctccc tttccacagc ctccaccctg gctgaactga aggtcatttc ctatagcttc
      961 aacattctga agtgttgtaa atacatttcc agtcaaaacg acatctctta ccatatcacc
     1021 aatcttacca ttttcaatca tataaccttc ctgtgcactg aaggtaaaca tctccataga
     1081 tgtctgtcct ccataagccc caacggcata tataccgtgt tttatatctg atataagctc
     1141 atcaaaagat gagcttccag gtactataaa tgtattactc atccttacta taggctggaa
     1201 cctgtagttt atcgagcggg cattacctgt aaccggttcc cccatctttc tggcagtctc
     1261 tctggagtgt aatctccctg agaggatacc ttccctgatc agatatgtct ttctgctcag
     1321 aatcccctca tcatcatatc tgtaagagcc tctcaaattt ggatatgtcg ggtcatccac
     1381 cacatttaaa aagtctggtc caaatctctt ccctatcaac ataagctctt taagtctttc
     1441 attctcatag acatggtctg cctcacttag atgaccaaaa gcttcatgga taaaaactcc
     1501 agccaatctt ggatccagga taacagtata ctgtccaccc ttaaccaccg gcgcagataa
     1561 cagtcttacc gccatctcag cggtatctct ggctttatca tggagatttt ccattatctg
     1621 aaaaccctct atgcttcctg tagaataatg atactgttct ataaggtctt cctcccttgc
     1681 cagggcgata agtacacttg atatatctcc cttttcctga actatatctg taccctctgt
     1741 gttaataaaa tatctcttat tgaatcggtc tccatacctg accacagtac tttgaatctt
     1801 tggactgaag ttaagcataa tatcgttata ttcactcaaa agtcttttct tttcttcgat
     1861 cggaatatcc ctgaagtctc gtcccatagg aaatacaacc ctatccacaa ccgggtctga
     1921 atactctatt tcaatctctt ctccagtgat aagattactg gcattacagg cctcctcaaa
     1981 gtaatccgta atagcttcag tatttatatc attaaaagag atgaagcccc agccgcatct
     2041 accaagaatt cttatatttc cacctacaga tttagataca gagagctcct ccagttcttt
     2101 tcccctaaaa cttatatttg ttacctcaga ttcttccaat ctcagctcac catatcttat
     2161 cctcttttcc ttagccagtt taagaaatat ttcctttatt tcttccacgt atccctcctt
     2221 atctaggctg ttattaatta agtttatcat aatcccttca aataagctac agtaaccccc
     2281 catccacctt cgttatactc tcccagtcga aatgactcca ccataggatg ctctttaaga
     2341 aagttagtca ccgctcttct gagaacccct tcccccttac catgtattat ataaatagga
     2401 gatacccctg ccagtaacgc atcatccaga tatttggata gcgtatccag agcctcttca
     2461 actgtgagct tcctcagagt aattctatta gagactgtcc tggatttatc cacctttatc
     2521 ttgagagacc catcactctg aggctgttct ttctcaacca ttactggtgt atagagttca
     2581 tccttagaaa catctacctt aagctttcct ataagaacgg tgaccttgct ttcatttata
     2641 tctattatct caccattctg ctttagagaa gggacataca cagtcatccc tgttctggga
     2701 gtggttattc tgtcaccctg aatttcaggt tcttccggga ctggctcaag gctctttatc
     2761 tcatccttta tagatgttat ttctcttcta ccggatgaac tcttcgccct gtcatataac
     2821 tcatccagac gaatctttgt ctgaaccacc agctctcttg cttcctcttt tgaccttctc
     2881 agtataatct tctgttgtgc agataactca tccaacaatt tttcatacct gtctttaagt
     2941 tcttcataat aacttctttc tcttttagcc tcttccagtt ctgaacgaag ttctagctcc
     3001 agctgatgaa cctggtccag taacctagtc aggctgagtt catcggttga tagaagtttc
     3061 tctgctctcc ttataataga ctcattcagt cctaattttc ttgcaataac aaatgcatta
     3121 cttacaccag gtctccctat acgaagttta tatgtgggta aaaatgtctg aggatcaaac
     3181 tccatcgccc cattcatcac tctatcctgc ttactcgccc atatctttag ttcatcgtag
     3241 tgactggcaa ctattaccct tgccccatta tctctcaatg cctctaatat acttatagcc
     3301 agagcagaac cctctctagg acttgtccct gtaacaggtt cgtctatcaa gaccatagta
     3361 tttctatctg catatcttat aaatggaata agatgtgtga gatgggcaga aaagctgctc
     3421 aaatcctgag cgatattctg ttcatcccct atctctacaa agacattttt aaatatcccc
     3481 atctctgcca gatctaccgg aagctgtaat cctgcctgtg ccatcataat taacagtccc
     3541 accgtcttca gtgtaaccgt cttaccaccg gtattgggac cacttataac caggatatca
     3601 aaactctcac caacctcaac atctacaggc acagccctat tccccagtat gggatgtctg
     3661 gcagatttaa atctcaccat tggcttatca atccatacag gggctataca gttccagcta
     3721 agggacaacc ttgccttggc tattataaag tcgatcctag agatagcatc cctagctgag
     3781 atcaactctt caccagagga agagaccctc aaagagagct ctccgagaat tctctgtatc
     3841 tcatactctt cttctttttt caattcctgg aggttgttat tcatatcaac catctcatat
     3901 ggttcaacaa agagggtact gccactttga gagacatcat gaacaatgct ctttatagcc
     3961 ctcttctgcg aagactttat cggaataaca taccttccat tccttatagt tatatatgat
     4021 tcctgtacaa aatctctcca ttccctgcct atgtgagatt caagatagga cctcacagac
     4081 tcttccaatc tggctattcc ccttcttata ccagcaagtt taggagacgc attgggcttt
     4141 atattaccgt cctcatcgat agcccttctt atatcgtcct ctaaagagga gagctcggga
     4201 atattatcag ccagttcttt gaaaagagca ctctttctga tatatctctt caccgaaggc
     4261 aatctggata taaattcagc tatatgtaac aactcctgtg gctcaaggat tactcccaga
     4321 ctagcctttt taaccagagg agatacatct actagccctg aaagactcgg ggaaggctcg
     4381 taagagatca atctcttagc ctcctcagtc tcagcagaat tcctcagtat ctcctccttc
     4441 gaggaggaag gactcagtct gagggcaata tccttcccca taggtgtctc tgttaaacta
     4501 cttaattttc ctaaaacaaa atcaaattca cataacctta aacttctctc atccatatta
     4561 attattttaa catattgggg tataattaag tatcaaggag gagagatcta tgccagagac
     4621 tttaattata aattttaacc cggcgcttga tagaaccgcg gtagttgata ggtataatcc
     4681 ctacggtgta aataaggcta aggaattgat agttctcccc ggtgggaaag gggtaaatct
     4741 cggaagggct ttaaagacat tgggttatac agatttcgtc tgctccggta taataggggg
     4801 aaatataggc aggctttata acgcccttct ggatagagaa ggaataccca atgatttctt
     4861 ctggataaga gatgaaacga ggatagcata tgcaacctat gaggaatcta caggtgtggg
     4921 agtaataacc aacgaacagg gacctaatgt aactgaagaa gagataacag ggttcaataa
     4981 ttttataatc actaaatata ctacagccaa tattataatc ctctcaggcg gttctccacc
     5041 aggtgttccc ccggaggata taaggggtct agttaatatc tttaagaata aagataaaca
     5101 gatatttata gatacaagtg gagatatact gagactctgt gcagatacag gtccatactg
     5161 ccttaagata aatgaagacg aacttaaaga cgccttcggt gtagatttag aagcgaaaga
     5221 tgctttaaga gaattttatt catctctagc aagactcggc acaaaatggc tcattgtgac
     5281 aagaggagaa aatggggctg tatttattgt tgataataag gtactctcag ggaaaaataa
     5341 aaggatatac tctcattatg ctattggttc tggagatgcc ttcctggctg gaatcttata
     5401 tggtagtctc aaaagatttt ctccagaaga gatactgacc ctggctatgt cctgtggatg
     5461 tgcaaataca ttacactttg gagcctgcgt atttgataga gaagattgcg aaaggataaa
     5521 gaatgagata attattgaag agtcgtatct ttaattcacc gattttttag atacgatggt
     5581 ttttatctcc tcttttaaag tggaaatctc atctttcagt atctttacct ctttttctaa
     5641 cctctctatc atttcatact ctata
//
LOCUS       DAOTKU010000042         5659 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_12918_length_5659_cov_4.854033, whole genome shotgun sequence.
ACCESSION   DAOTKU010000042 DAOTKU010000000
VERSION     DAOTKU010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5659
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12918_length_5659_cov_4.854033"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="P5310_01785"
     CDS             complement(<1..717)
                     /locus_tag="P5310_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013290710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HRU32129.1"
                     /translation="MLKARMTLDCEYRTGSLDRRVFGSFIEHLGRAVYTGIYEPGHPS
                     ADEEGFRKDVVELVKDLNVPIIRYPGGNFVSGYNWEDGVGPKEKRPKKLDLAWRSIEP
                     NEVGINEFASWSRKVNSEVMMAINLGTRDVDAARNIVEYCNYPGGSYYSDLRKLHGVK
                     EPHNIKVWCLGNEMDGPWQIGHKTAVEYGRIAAESAKVMKWVDPDIELVVCGSSNPWM
                     PTYPEWEYTVLDLTYEYIDYI"
     gene            complement(753..2657)
                     /locus_tag="P5310_01790"
     CDS             complement(753..2657)
                     /locus_tag="P5310_01790"
                     /inference="COORDINATES: protein motif:HMM:NF017215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HRU32130.1"
                     /translation="MILGIDTGGTYTDSVILDEDRVFSKGKALTTHYDLTKGIRESID
                     ALDKNLFPEVSAVILSTTLATNTIVEMRRLERVKEVLIGYDRDFFLKNISNIPEWLEF
                     EFVSGSIDVTGEEVLPLDLERIKSLEVGDYPLAISGYMSPINPKYEMMAKDILKSKSR
                     YPVVCGSELSGELNSIKRAITTGLNASLIPVISSFIRDVKRVLAEEGIKARILIVNAD
                     GSITDVEDAMEKPIKTVLSGPASSTIGGLFLSGIDNGIIVDMGGTTTDISLVQGSAPL
                     ISSEGARVGEWELLLPSLKMRTVGLGGDSRIHLLKNSIEIGPARAIPVSVLGKTFPEI
                     KKKLKKTHNSSLVPPNEFIVKLREPSFELNSIERKLMGSLNGEPLSMEEATKICNLSH
                     PYILDSAIKRLEEMGIIIRSGLTPTDILLYLGECEMGDPSISSIALSAFSKEKEFPQF
                     IKKLIIKKIATEILRYLFFLDYKDERIDGLLGNLINDSLDRRFNLIETGIKLPFPIVG
                     IGAPVKSFIYEVAEMLHTSAIVPEHYEVANAAGAGWGRVIRSIELEIMPIVSSAMIKG
                     YKIRGLDRKFNEYEKAKNFAINYGSELIESQLKKLTGDDGEIKTEVIFNDSDETALKK
                     VQVIGMGKIQ"
     gene            complement(2654..3010)
                     /locus_tag="P5310_01795"
     CDS             complement(2654..3010)
                     /locus_tag="P5310_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase subunit A-like
                     protein"
                     /protein_id="HRU32131.1"
                     /translation="MAKKGLIFCVCQGTCPSFQQMNIFEVVNTLRMEKLVDFVGIQPQ
                     LCAEDGDSFFEILLSGINDKIDKLVIAGCDPNMQRKMFRDAFENAGFDKSKHVGVDIR
                     NMTTEQAMEAIKKVLI"
     gene            complement(3015..3224)
                     /locus_tag="P5310_01800"
     CDS             complement(3015..3224)
                     /locus_tag="P5310_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRU32132.1"
                     /translation="MARKWYPVIDYDKCIRDLVCANFCPHGVYAIDDNKSLVVNPDGC
                     IEFCRGCQKICPADAISYFGDKEAK"
     gene            complement(3240..3596)
                     /locus_tag="P5310_01805"
     CDS             complement(3240..3596)
                     /locus_tag="P5310_01805"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRU32133.1"
                     /translation="MESTLYNVLMQKFEYNDKLINLIWDKLQALANPIRLKILLALKE
                     KPMCVCELSEYLGDRQPLISQHLAILKDSDLVIAKRMGTRIQYSLSDNKIIELIDIIK
                     ELTIKKIEELTTLKEE"
     gene            3777..4547
                     /locus_tag="P5310_01810"
     CDS             3777..4547
                     /locus_tag="P5310_01810"
                     /inference="COORDINATES: protein motif:HMM:NF012669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HRU32134.1"
                     /translation="MRETSSRERRDKILEILIENDQVRVSSLCKLFNVSRETIRHDLI
                     RLQEEGLIIKEHGSAALNRESILDIDFAKRARDHAVQKRRIGLEAAKLIKDGETIIID
                     SGSTTPHIARNIRNIKSLTVITPSLKVTSELGGKEGITVIMPGGIFNPTGYSLYGPQT
                     EEFFPKINANKLFLAIHGVDIERGLTEINIQIAHLKQVMINSAKEIILVADSSKFGRI
                     EYAGVAPIDKVNAIITDRDLDKGIAEEIQSLGINLILV"
     gene            4558..>5659
                     /locus_tag="P5310_01815"
     CDS             4558..>5659
                     /locus_tag="P5310_01815"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HRU32135.1"
                     /translation="MSNKTFLRVGLFFSVLLLSLLLTSSVYAKSYWLPQVDCRIDVQE
                     DGSLLWRYSLRFSFSGKFSYAYMDIPTAGIKIEEVSSPEKISIENRGDHIRVRWDFSA
                     ENEEKTFNLSYKMSNVLKVYNDIAEFYWKIWSEGWDVKVGKLNVKVYLPGKVSHRDEL
                     YIWGHPRLPGRVDILNTLDGFSLETEDISSNQWVEIRALFPATVLSSYSGAIRINKDM
                     LEDILREEENFTRQTEKEKYYLSLAISGGLGVFLCMIVILIYLYFIYGREPRINYQGI
                     YEREIPYDYSPAIVGAIMNLRTMTPGTRDFTATLLDLVHKGYIDMTPVGDDYILSLKE
                     KDRSNLEPFEMYILEYLERIKRKEVEGLLFSDL"
BASE COUNT         1699 a         1182 c         1002 g         1776 t
ORIGIN      
        1 gatgtaatct atatattcat aggtaaggtc cagtacggta tattcccact caggatatgt
       61 tggcatccag gggttggaac tcccacatac caccagttca atatccgggt ctacccattt
      121 cattaccttg gcagattctg ccgctatcct tccatattct accgctgtct tatgaccgat
      181 ctgccaggga ccatccatct cattacccag acaccatacc tttatgttat gaggctcttt
      241 taccccgtgt aatttcctca ggtcgctgta ataagaacca ccaggatagt tacagtattc
      301 aactatattt ctggctgcat ccacatccct ggtacccagg tttattgcca tcataacctc
      361 agagtttacc tttctggacc agctggcaaa ctcatttata ccgacttcgt ttggctcgat
      421 acttctccat gccaggtcga gcttttttgg tcttttctct ttaggtccta caccatcctc
      481 ccagttataa ccagagacaa aatttcctcc aggatatctt ataatgggga catttaaatc
      541 ttttaccagt tctactacat cctttctaaa accttcctca tctgcactag ggtgtcctgg
      601 ttcatatata cctgtatata ccgctctgcc aagatgttca ataaaagaac caaagaccct
      661 tctatccaga cttcctgttc tgtactcaca atcaagagtc atccttgcct taagcataaa
      721 gacctcctct acaagatatt tttaatatta tactattgta tctttcccat tcctataacc
      781 tggacctttt taagagcagt ctcatcggaa tcattaaaga tgacttcggt ttttatctct
      841 ccatcatccc cagttaactt ttttaactga gattctataa gttcacttcc ataattaata
      901 gcaaaattct tagccttttc atactcatta aattttctat ccagacctct tatcttatat
      961 ccctttatca tagctgatga tacgataggc attatctcca gttctattga ccttataact
     1021 ctcccccatc cagctcctgc agcatttgcc acctcataat gttccggtac tatggcagac
     1081 gtatgtagca tctctgccac ttcatatata aagcttttaa caggggcacc tatccctact
     1141 atagggaacg gaagttttat cccggtttct atcagattaa atctcctatc caggctatca
     1201 ttaattaaat tacccagaag tccatctatc ctctcatctt tataatctaa gaagaaaaga
     1261 tatcttaata tctcagttgc tatcttcttt attataagct tcttgataaa ctgtggaaat
     1321 tccttctctt tagaaaatgc tgatagggct atactgctga tagatggatc tcccatctcg
     1381 cattcaccaa gataaagaag tatatctgtt ggggtaagac cagacctgat gattatcccc
     1441 atctcctcca acctctttat agcagaatcc agaatataag gatgactcaa attacaaatt
     1501 ttggtagcct cttccatcga tagtggttca ccgttcaggc tccccataag ttttctttct
     1561 atactgttca gttcgaatga tggttctctc agtttaacta taaactcatt ggggggtacc
     1621 agagatgagt tatgagtctt ttttaatttc ttctttatct cagggaatgt tttccccagt
     1681 accgataccg ggatagctct tgctggacct atctctatag aatttttcag aagatgtatc
     1741 ctgctatctc cacctaaacc taccgtcctc attttcagag agggtaagag taattcccac
     1801 tctcccaccc ttgcaccctc gcttgatatt agcggagcag aaccctggac taaagatata
     1861 tctgttgttg ttcctcccat atccactatt atcccgttat ctatacctga caggaataac
     1921 cctccaatgg tactgcttgc tggaccggat agcacagtct ttataggttt ttccatagca
     1981 tcttcaacat ctgttataga accgtcagca tttacaataa gtattcgggc ttttataccc
     2041 tcttcagcaa gcaccctttt tacatccctg atgaagctgg agattaccgg tataaggctg
     2101 gcgtttaacc ctgtggttat agcccttttt atagaattca gttcacctga gagttcgctc
     2161 ccacatacaa caggataacg ggatttactc ttcaggatat cttttgccat catctcatac
     2221 ttaggattta tcggactcat atagccggag atagccagag gataatcccc aacctccaaa
     2281 ctttttatcc tttccaggtc taacggcaaa acctcttccc ctgtaacatc tatactgcca
     2341 gacacaaatt caaactcaag ccactctgga atattagaga tattctttaa gaagaaatct
     2401 ctatcataac ctataagaac ctcttttacc ctttcaagtc ttctcatctc aacaatagta
     2461 ttggttgcca gtgtggtgga gagaataaca gcagagacct ctggaaaaag attcttatca
     2521 agagcatcaa tactttctct tatccctttt gtaagatcgt aatgagttgt taatgccttc
     2581 cccttagaaa aaactctatc ctcatctaaa ataacagagt ctgtatatgt tccacccgta
     2641 tctatcccaa gaatcatata agaactttct ttattgcctc catagcctgt tcagtagtca
     2701 tatttctgat atctactcca acatgcttgc tcttatcgaa acctgcattt tcaaaagcat
     2761 ccctgaacat ctttctttgc atattggggt cacatccggc aataacaagt ttatctatct
     2821 tgtcattgat tccggaaaga agaatctcaa aaaaagagtc cccatcctca gcgcagagtt
     2881 gaggctgaat tcctacaaag tctaccagtt tttccattct gagagtgttt accacttcga
     2941 aaatattcat ctgctgaaaa gatgggcagg tcccctgaca cacacaaaaa attaaacctt
     3001 tcttagccat ttttttactt tgcctcctta tcaccaaaat atgaaattgc atccgctggg
     3061 catatcttct gacatcccct gcagaattcg atacatccat ctggatttac tacgagtgac
     3121 ttattgtcat caatagcata taccccatga ggacaaaaat tagcacagac cagatccctg
     3181 atacatttat cataatcaat aactggatac catttacgcg ccataatatc tacaaatact
     3241 tattcctcct ttaacgtggt aagttcttct atctttttaa ttgttaactc tttaataata
     3301 tctataagtt ctataatttt attatcgctt aggctgtatt gaattctggt gcccattctc
     3361 tttgctataa ccagatcgct atctttaagt attgccagat gctgagatat gagaggttgt
     3421 ctatcaccca aatattctga taactcacat acacacatgg gtttctcttt taaagccaaa
     3481 agaatcttca atctgatagg attggctaga gcttgaagtt tatcccatat tagattaatt
     3541 agtttatcgt tatattcaaa tttttgcata agtacattgt aaagagttga ttccattatg
     3601 tcaatagcct gtaaagagag gggggaccac ctttcaaggg aaatgatgaa atagaaatta
     3661 tcctacaagg gtggaataaa acaaacccta tgcatcaccc gtcaccgctt ttcagaatta
     3721 ccaactccct ctattctatt atgtgataga ataataaaat agaaaggaga tgagagatga
     3781 gagaaacaag ttccagggag agaagagata agatacttga gatattaata gaaaatgacc
     3841 aggttcgggt ctcaagttta tgtaagcttt ttaatgtcag tagagaaaca ataaggcacg
     3901 acctgatcag actccaggaa gaaggtctaa taattaaaga gcatggttct gcagcattaa
     3961 atagggaatc catcctggac atagattttg ccaagagagc aagagaccat gcagttcaga
     4021 aacgcaggat aggtttagag gcagctaaat tgataaaaga tggggagact attattatag
     4081 attcaggcag tactacccca catatagcaa ggaacatcag aaatattaag agtcttactg
     4141 tcattacacc atctctaaag gttacatcag aattaggtgg taaagagggg ataactgtaa
     4201 taatgcctgg tggaatattt aatcctacag gatactcgct gtacggtcct cagactgaag
     4261 agtttttccc gaagataaat gccaataaac tctttcttgc tatccatgga gtggatattg
     4321 agagaggatt gactgaaata aatatacaga ttgctcattt gaaacaggta atgataaata
     4381 gtgccaaaga gataatcctt gtagcagact ccagcaagtt tggaagaatc gaatatgcag
     4441 gggtagctcc catagataaa gttaatgcta taatcaccga tagagacctg gataaaggga
     4501 ttgcagagga gattcaatcg ttaggtataa atcttatact ggtatgaggt gggttagatg
     4561 agtaataaaa ctttcttaag ggtaggttta tttttttctg ttctgctcct ctctctactt
     4621 ttaacttctt ctgtttatgc taagagctac tggcttccac aggtagattg tagaatagac
     4681 gtgcaggaag atggtagtct tttatggaga tactcactga ggttctcttt tagtggtaaa
     4741 ttttcatacg cttatatgga tatcccaact gcagggatca aaatcgaaga ggtttcttct
     4801 cctgaaaaaa tctctataga aaacagaggg gatcatatca gagtcaggtg ggatttctcg
     4861 gctgaaaatg aggagaaaac atttaatcta tcttataaga tgagtaacgt ccttaaagtt
     4921 tataatgaca ttgcagagtt ctactggaag atctggtcgg aaggctggga tgtgaaggtt
     4981 ggtaaattaa atgtgaaagt ctatttacct ggcaaggtca gtcatagaga tgaactatat
     5041 atctggggac atcccagatt acctggacgg gtagatatac tgaatacttt ggatggcttt
     5101 tctctggaga cagaagatat atcttctaat cagtgggtag agataagagc tctcttccct
     5161 gctacagttc taagctctta ttctggagca attagaatca ataaggatat gctggaagat
     5221 atattgaggg aagaagagaa tttcacaagg cagacagaaa aagaaaagta ttatctttct
     5281 ctcgccataa gtggaggtct tggtgtcttt ctctgtatga tagttatcct gatatattta
     5341 tacttcatat atgggagaga accaagaata aattatcagg ggatatatga aagagagatt
     5401 ccatatgatt attctcctgc gatagttgga gccattatga atctaaggac tatgactcca
     5461 ggcacaagag attttacagc cacattgctt gaccttgttc ataaaggata tatagatatg
     5521 acaccggtgg gagatgatta tatactttct ttaaaagaaa aagacaggag taatttggaa
     5581 ccttttgaaa tgtatatcct ggaatatcta gaaaggatta aaaggaaaga agttgaggga
     5641 ttactatttt ccgacctgc
//
LOCUS       DAOTKU010000043         5657 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_12926_length_5657_cov_3.270439, whole genome shotgun sequence.
ACCESSION   DAOTKU010000043 DAOTKU010000000
VERSION     DAOTKU010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5657
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12926_length_5657_cov_3.270439"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..857
                     /locus_tag="P5310_01820"
     CDS             <1..857
                     /locus_tag="P5310_01820"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRU32136.1"
                     /translation="FLGPNGAGKTTTIMMILGILKPTSGKILVFNEDLYSDYFGIKRR
                     IGVVSEVQYLYEDMTAYEYLSFFCDIYNVKNKREKIEELLHKVDLYDRKDDLLGHYSR
                     GMQQKIGFVRALLNDPELLILDEPVSGLDPTGIREVRDLILEENQRGRTVFMSSHILS
                     EVERISHRVGIMNKGRLVAEDTMENLRKKLSSEVEIELEVDNFDVEAEKRLESLSFVN
                     SVTKEDGKYLVRVKADKDYRGDLVNFVSAIGGNLKEIRKREMSLEDAFITITEKNISL
                     FSSGEVSE"
     gene            854..1609
                     /locus_tag="P5310_01825"
     CDS             854..1609
                     /locus_tag="P5310_01825"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRU32137.1"
                     /translation="MSYRWTVIKSIMKREARSSFFSLGLYIAATIACLVSAFIFNTYI
                     NTVEGNNIMVMASPLNTPLFYAVIVAALYAAISSSTALSREKDRGTLEVLFYGPVDHL
                     SVILAKFFEDIISFLIIMLLVTIYFAYAAITTNLGFSITYLYAIILSIFIASCMSSFG
                     LWLSSLTARIRTSILLLIVVMGGLLGLQFLQNYILTLKPENLSLSMVYIMGIVSNILR
                     VIKWVSPFSYLTDGINAVALGSVSMYIENMIEQ"
     gene            1674..2960
                     /locus_tag="P5310_01830"
     CDS             1674..2960
                     /locus_tag="P5310_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32138.1"
                     /translation="MRKTCCISALILIFIILSSAIGFSAETPKSKEVVYSLNVWDGKD
                     YAAPFYPSAYDTIYVMADYENVYSVKETLVYYWPLTREYMADWDGLNKDVGETIEVLK
                     GNEIIGTYKKVDYVFYYPKGYWGGGTQLFTGDKAKEKKKEYDQAVNKYWKEVEAYYQA
                     YEKYNKEVEEFYQNIQEGKPAGKIPQEPAPPTAPTFYVTDISKAYVFSLPPGQYTIRT
                     KDKNEEIIPGTVKNLIAFSHRREGIGYNIIPESKWTYPEVADDSSEIVYQYREGTLYF
                     QPYKEWEFNELYYNKLSKPQSPGRSDRWIWVHMDPASNVKLRIYSGDSIFSEIRNRPY
                     YVEQVPGSALGYNIKLLDRKENPYGMADFSAFKFSVPPTGDYKLVTIDSKGDIIENSD
                     RYIRPITVTDARNIFIASLGPLIIGLVIYIIRRVGR"
     gene            2969..3364
                     /gene="rpsI"
                     /locus_tag="P5310_01835"
     CDS             2969..3364
                     /gene="rpsI"
                     /locus_tag="P5310_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004101630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HRU32139.1"
                     /translation="MIGNYYWGTGRRKESVARVRITEGDGKIVINGDKELQDYFSLER
                     WQTQILSPFRITGTEGRFDCYVNVNGGGVNGQAEAVRLGVSRALVEYNPEFRPVLKEY
                     GLLTRDPRMKERKKYGLKRARKAPQFSKR"
     gene            3366..3809
                     /gene="aroQ"
                     /locus_tag="P5310_01840"
     CDS             3366..3809
                     /gene="aroQ"
                     /locus_tag="P5310_01840"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HRU32140.1"
                     /translation="MQILVINGPNLNRLGLREPEIYGRFTMKDLENLIREEGEALGLK
                     VDFFQSNSEGAIIESIQNAKNKYDGIIINPGAYTHYSIAIRDAIAGENIPTVEVHISN
                     IYKREEFRHHSLIAPVAIGQIAGFGIYGYTLALYGLNNYLRGKYV"
     gene            3802..4875
                     /locus_tag="P5310_01845"
     CDS             3802..4875
                     /locus_tag="P5310_01845"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HRU32141.1"
                     /translation="MSKTERLNNLRERFTRFDAFITSKPVNLRYLVDFKGSDSILLVT
                     RNKVQLYVNEMYYEQAQKDCTGIDIITVDRSWIELLNKDLKKEKIKRLAFESDIDFST
                     YRKLNSFLNGIELIPVEDLVESVRAVKDEEEIGFIRKAAEIADLAFMHLKTLIKPGIK
                     EQDLVAEFDYFVRKRGAKVSFEPIILYGESSSLPHGISGAREIKSGGILLLDYGAMYN
                     GYCSDATRTFYLGKPSKEYLKLYNIVLKAQEEAESAIRPGVSAGEIDRIARDVITQEG
                     FGDKFIHSTGHGVGLEIHEYPRLYWGNNVILEKGMVITVEPGIYIKDSGGIRIEDLVV
                     VTDDGGEIITRISKDFEFRGGNI"
     gene            4872..5435
                     /gene="efp"
                     /locus_tag="P5310_01850"
     CDS             4872..5435
                     /gene="efp"
                     /locus_tag="P5310_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HRU32142.1"
                     /translation="MISTNDFRPGITVEIDGEIYEVVEFLHVKPGKGSAFVRTKLRNI
                     KTQAVIERNFRAGEKFPRAILERREMQYLYNQDDIYHFMDTTNYEQVSLSKEVIGDAT
                     KFLKENILVYIVFHNGTPIGIDLPITVELEVVSTDPGFKGDTVTGGSKPATLETGAVI
                     QVPLFVNVGDIIKIDTRTGEYLERVNK"
     gene            5453..>5657
                     /locus_tag="P5310_01855"
     CDS             5453..>5657
                     /locus_tag="P5310_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32143.1"
                     /translation="MSNLMDKVDMLKSLGDNVTLSDIVRVLEAVVEELNSIKLTINNI
                     KEQVDAVDTDLSNLENAVYGEIEI"
BASE COUNT         1863 a          838 c         1328 g         1628 t
ORIGIN      
        1 gttttttggg tccgaatggg gcagggaaga ccaccactat tatgatgatt ttaggtattc
       61 ttaagccgac cagtgggaag attctggtgt ttaatgagga cctgtatagt gattattttg
      121 ggataaagag aaggataggg gtagtctcag aggttcaata tctctatgaa gatatgactg
      181 cctacgaata tctctcattt ttctgtgaca tttataacgt aaaaaacaag agagagaaga
      241 ttgaggagtt actgcacaag gtagacctgt atgacaggaa agatgacctc cttggacatt
      301 attccagggg aatgcagcag aagataggtt ttgtgagagc tttgttgaat gatcctgaac
      361 tgcttattct tgatgagcca gtttcaggtc ttgatccaac cggaataaga gaggtgagag
      421 acctcatact cgaagagaac caaaggggta gaacagtatt tatgtcttca catatattat
      481 cagaggtaga acgtatctca cacagagtcg gaataatgaa taaaggcaga ttagtagcgg
      541 aagatactat ggaaaacttg aggaagaaac tttcaagtga ggtagagata gaattagagg
      601 tcgataattt cgacgtggaa gcggagaaga gattagaatc actttcgttt gtgaatagtg
      661 ttactaaaga agatggtaaa tatctggtaa gggttaaagc tgacaaagat tatcgtggag
      721 acctggtaaa cttcgtatcc gctattggtg gaaatcttaa agagataaga aaaagagaga
      781 tgagtttaga agacgctttt attactatta ctgaaaagaa tatatcttta ttctcttctg
      841 gggaggtttc agaatgagct acaggtggac tgtaataaaa tctattatga aacgtgaagc
      901 ccgttcaagc tttttcagtt tggggctcta catagctgct accatagcct gtcttgtatc
      961 tgcatttata ttcaatactt atataaatac tgtagaaggt aataatatca tggttatggc
     1021 ttctcctcta aatactcctc ttttctatgc ggttattgta gctgcacttt atgctgccat
     1081 atcatcttct accgctctat ccagagaaaa ggatagggga acgttagagg tactgttcta
     1141 tggtccagtt gaccatcttt ctgttattct tgcgaagttc tttgaggata taataagttt
     1201 tttgataata atgttgcttg taacaatata ttttgcctat gcagcgataa ctacaaatct
     1261 tggattttct ataacttacc tctatgcaat tattctatct atttttatag ctagctgtat
     1321 gtcctctttt ggtctctggc tttcttctct tactgcccgt atcaggacat caatactgct
     1381 cctcatagtg gttatgggtg ggctactcgg tcttcaattc ctccagaact atattctcac
     1441 attaaaacca gaaaatttat ctctctctat ggtatatatt atggggatag tatctaatat
     1501 cctcagggtc attaaatggg tatcaccatt cagttatctg acggatggta taaatgcagt
     1561 ggctctgggt agcgtaagca tgtacataga gaatatgatt gagcaataat ttatacagtt
     1621 gtgttactta cagctacagt gtacacgtta aaatggaagg gggtaagaaa actatgagaa
     1681 agacctgctg tatatctgct ttaatcctaa tttttataat tctctcttca gctattggat
     1741 tcagtgctga gactcctaag agtaaggagg ttgtatacag tcttaacgtg tgggacggga
     1801 aagattatgc tgcacccttt tatccttctg cttatgatac catatatgta atggcagatt
     1861 atgaaaatgt ctactctgtt aaggaaaccc ttgtctatta ttggcctcta accagggagt
     1921 atatggcaga ctgggatgga cttaataaag atgttggaga gaccatagaa gttttaaaag
     1981 ggaacgagat aataggtact tataaaaagg tagattatgt attctactat ccaaagggat
     2041 actggggtgg agggacccag ctatttaccg gtgataaggc aaaggaaaag aagaaagagt
     2101 atgatcaagc ggtaaataaa tactggaaag aggtagaggc ttattatcag gcatatgaga
     2161 aatataataa ggaggtagaa gagttctatc agaacataca ggagggaaaa cctgccggaa
     2221 agattcctca ggaaccagct cctcctacag ctccaacatt ctatgtgaca gatatctcaa
     2281 aggcgtatgt attcagttta cctcctggac aatatactat aagaacaaag gataagaatg
     2341 aggaaattat acccggtaca gtcaaaaatc ttatcgcttt ctcacacagg agggaaggga
     2401 taggatataa tataatacct gaaagtaagt ggacatatcc ggaagtggcg gatgactcca
     2461 gcgagattgt ctatcagtat agagaaggaa cactttactt ccagccctat aaagagtggg
     2521 aatttaatga gctgtactat aataaacttt ctaagcctca gtctccagga agatcagata
     2581 gatggatatg ggtccatatg gatccggcat ccaatgtaaa actcaggata tacagtgggg
     2641 atagtatatt ttctgagatc agaaatagac cttactatgt agagcaagta ccagggtcgg
     2701 ctttaggtta taatataaaa ttactggaca ggaaagagaa tccttatggt atggcggatt
     2761 tttccgcctt taaatttagt gttcccccaa ctggggatta taaactggtg acgattgata
     2821 gcaaaggtga tataatagag aacagtgata gatatataag acctattact gttactgatg
     2881 caagaaatat ttttatcgca agtcttggac cgttaattat tggtctggtg atatatatta
     2941 taagaagagt aggaaggtga tatagaagtt gataggtaat tattattggg gaacaggaag
     3001 aaggaaagag tcagtagctc gagtaaggat tactgaaggg gatggaaaga tcgtcataaa
     3061 tggcgataag gaattacagg actacttctc ccttgaaaga tggcagacac agatactttc
     3121 gccattcagg ataacaggaa cagaaggaag gtttgactgt tatgtgaatg ttaacggtgg
     3181 tggggttaat ggacaggctg aagcggtaag gcttggagta tcaagagctc tggttgagta
     3241 taatccagag tttcgtccag ttcttaaaga atatgggctc cttacccgtg atccaaggat
     3301 gaaagagaga aagaaatatg gactcaagag agctagaaag gctccccaat tctcaaagag
     3361 ataatttgca gattcttgtg ataaatggcc ctaaccttaa caggttaggg cttagagaac
     3421 ctgaaatata tggaagattt accatgaagg acctggagaa ccttataaga gaagagggag
     3481 aagcgctggg tttgaaagta gatttttttc agtctaacag tgagggtgca ataatagaga
     3541 gtattcagaa tgccaagaat aagtatgatg gtattataat aaatcctggt gcctatactc
     3601 actatagtat agccataaga gatgcgatag ctggtgagaa tatcccaact gtagaagtac
     3661 atatatcaaa tatatataaa agagaagaat tcaggcacca ctctttaata gcaccggtag
     3721 ctattggtca gatagctgga ttcggtattt atggatatac tctggcgtta tacggattaa
     3781 ataattattt gagaggtaag tatgtctaaa actgaaagat taaataattt gagagaaaga
     3841 tttacaagat ttgatgcctt tattacatca aaacctgtaa atttgagata tctggttgat
     3901 tttaagggga gtgactctat acttctcgtt accaggaata aagttcagct ctatgtaaac
     3961 gagatgtatt atgaacaggc acagaaggat tgtactggaa tagatataat tactgtagac
     4021 aggagctgga tagaattatt aaataaagat ctcaagaagg aaaagataaa gagactggct
     4081 tttgaatcag atatagattt ttctacctac aggaagctta attccttcct taatgggatt
     4141 gaacttattc cggtagagga cctggtagag tctgttagag cggtaaaaga tgaggaggag
     4201 ataggtttta ttcgaaaggc tgcagagata gctgatctgg cttttatgca tctcaaaacc
     4261 ttgataaagc ccggtattaa agagcaggat ctggttgctg agtttgatta ctttgtaaga
     4321 aaaagaggcg caaaagtcag ttttgagcca attattctat acggagagtc gtccagtctt
     4381 ccacatggca tctctggcgc aagagagata aagagtggtg ggattcttct gctggattat
     4441 ggtgcaatgt ataatgggta ctgttcggac gctaccagga cattctattt gggaaagccg
     4501 tcgaaggaat acttaaaatt atataatata gtattgaaag ctcaggaaga ggcggaatct
     4561 gctataagac ccggtgtatc cgctggagag atagatagaa tagccagaga tgttataacc
     4621 caagaaggat tcggggataa gttcatccat tctacaggac atggtgtggg attagagata
     4681 catgaatatc ccagactata ttggggcaat aatgtgattt tagagaaagg gatggttata
     4741 actgtagagc caggtatata cataaaagat tctggaggta taaggataga agatcttgta
     4801 gtggttactg acgatggtgg agagattatt acaaggataa gtaaagattt tgaatttaga
     4861 ggaggaaaca tatgatctca acgaatgact ttcgtcctgg tattaccgta gagatagatg
     4921 gagaaatata tgaggtggta gaattcttac atgtgaaacc cggtaaggga tcagcctttg
     4981 taagaacaaa acttagaaat ataaaaactc aggcggttat agagcgtaat ttcagggcag
     5041 gggaaaagtt ccccagggct atcctggagc ggagagaaat gcagtatctc tataatcagg
     5101 atgatattta ccattttatg gataccacta actatgagca ggttagtctg tctaaagaag
     5161 ttatcggaga tgcaacgaag tttttaaaag agaatatcct ggtttatata gtattccata
     5221 atggaactcc tatcggtata gaccttccca tcacagtaga gttagaagta gtaagtacag
     5281 accctggatt taaaggggat actgtaacag gtggtagtaa gccagcaact cttgaaacag
     5341 gagcggttat tcaggtccca ctttttgtca atgtgggaga tattattaaa atagataccc
     5401 gaacaggcga ataccttgaa cgggtaaaca aataggagga atacgagata atatgagcaa
     5461 cttaatggat aaagttgata tgcttaaatc attaggggat aatgttacac tatcagatat
     5521 agttcgagtt ttggaggcgg tagttgaaga acttaattct ataaaactta caattaataa
     5581 tatcaaagag caggttgacg ctgtagatac cgatcttagc aatttagaaa atgccgttta
     5641 tggagagata gagattg
//
LOCUS       DAOTKU010000044         5655 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_12931_length_5655_cov_3.107857, whole genome shotgun sequence.
ACCESSION   DAOTKU010000044 DAOTKU010000000
VERSION     DAOTKU010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5655
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12931_length_5655_cov_3.107857"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="P5310_01860"
     CDS             <1..536
                     /locus_tag="P5310_01860"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRU32144.1"
                     /translation="SKMEKVLDSFFRFTLFHMEAWAKTSAEVIIQHDDFVWTGGPFMR
                     PEIYRKVIIPRYAELWKPLHRSGKKVLFCSDGNFMGFAEDIVNAGADGLIFEPCNDFG
                     FMVERFGGSTVLVGSFVDCRDLTFGKWEVVRASIDKTVELAKKFKGIMFAVGNHLPPN
                     IPSEMMERYIGYLKEVL"
     gene            533..>1288
                     /locus_tag="P5310_01865"
     CDS             533..>1288
                     /locus_tag="P5310_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32145.1"
                     /translation="MNKFRVPFEKPEPNFSEFRDVILGKKPARRVHFAELFPDIEIIW
                     YITENILGDKVIHYPQDDFQGFWRQQINFWYRMGYDYIRVSGGLGFAEQKRRVAEDTA
                     ILPRGQRNWIEESVGIITSWKDFEEYPWPKSESFDYSNYEFVLKNLPEGMKMLVCPSS
                     GVFEISSESLLGFEGMSYLLYEDYELVKAVFDRVGETIYRFYENLITLDNVEGIFQGD
                     DLGFKTSTILSPKYLRALVLPWHKRYAELAHRYN"
     assembly_gap    1289..1298
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1299..1505
                     /locus_tag="P5310_01870"
     CDS             <1299..1505
                     /locus_tag="P5310_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III decarboxylase-like protein"
                     /protein_id="HRU32146.1"
                     /translation="GDRVAALGGVDVDKLCRLEERELRSYVRSILECCMPTRYALGSG
                     NSVTNYVPVENYLIMLDEGVRYCD"
     gene            1498..3477
                     /locus_tag="P5310_01875"
     CDS             1498..3477
                     /locus_tag="P5310_01875"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HRU32147.1"
                     /translation="MIELPFRQVHLDFHTSPLIPDVGVDFDPEEFAKTLKDAYVNSIT
                     VFAKCHHGMSYYPTKVGMVHPSLKRDLLGEMVEACHREGIRVCAYISVVWDEYSASKH
                     ADWLQVNKDGTLAGRPPFGNRGWRFLCMNSPYVDYVAEQTEEVLKSYDVDGVFFDIVM
                     QTFPGCVCNHCMDSMKRLGLNPEDDKDLYRHSLIVSRNFMDRMDKLVKSLNSDASIFY
                     NSRLRLESDDEFGMRPEADYFTHFEIESLPSGGWGYNHFPLFVRYYQTLDKDLLGMTG
                     RFHKSWADFGGFKNKPALEYECFTMLSLGAKCSVGDQLHPRGKLDKTVYNLIGSVYKK
                     VAEREEWCEDAKPLSDIGVLISSSFFNDWRTDSDEGAMRILSEFHYQFSFIDSREDLS
                     KYKLIIAPDRVSMDEVLAEKLRKYLNGGGKLITSYKSGLAGDKFILDDLGVEYLGDSD
                     YCPDYFRVLSPADKEIEPAEYVMYERGTKVQPIDGTQILAETYQPYFNRTWEHFSSHA
                     QTPPDKKTPYPAAVRNDNVIYISYPIFRAYILNGNLVYKKLVRNCIDLLLDNPLTIDN
                     APSTARITVTSQHYNGKDRHIVHILHYIPERRCKSIDVIEDVIPICNINLKVRVDGSP
                     SRCYLVPEKKEVNFVYNNGYVEFTVPEVNGYQLWL"
     gene            3499..4995
                     /locus_tag="P5310_01880"
     CDS             3499..4995
                     /locus_tag="P5310_01880"
                     /inference="COORDINATES: protein motif:HMM:NF019496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose/L-arabinose isomerase family protein"
                     /protein_id="HRU32148.1"
                     /translation="MRSTPIGIVLMNDGRPHVYSANEEENMQVVRRWAEVIKKNLKNI
                     DGSSPEVVIASETIKSVRTAQKIAEEFNKANCKQVVMCYNVWNFPYLAWPFLNSLDVN
                     IPILSLSNNNAQYPGNVGLLATDGEIRQTGRRTHRIVGDVDDPKIQEKVIRWLRASQA
                     VTTMRGEVYGLYGGHSMGMETGYFHLVPNIKTFGVNTYQIDQLLLVKVMEEIDDKEVE
                     KGFQWFTELLGDRILYDNKMLTPETLKTQIRLYLAMKKVNEENGFDFCGLKGQRELTE
                     YVCLGDIPEMLMNDPYDWYGFKEPTVCATEADSFAALTMEVLKYITGGLPVLFMDVRI
                     YYPDKDLWDFCNSGNHASYYASLSFDPKENFKKITLYPALGYYFKTGGASVSFDAAPT
                     KMTFARFGLYNDKPYMVIIPGESLDLPEEERKAINKLSDPTWPHVHARLNCSFDEFLN
                     VFPANHIQAIPGDYVDALVYYCEILGIKPIVLGPRSKERLTPIWEILE"
     gene            5166..>5655
                     /locus_tag="P5310_01885"
     CDS             5166..>5655
                     /locus_tag="P5310_01885"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRU32149.1"
                     /translation="MIRIVDIAEKANVSPGTVSKILKGTYIGSQETKERVLKIAKDLG
                     YTPNIIASSLKSRRTRIIALIVPEISVPFFPELSRGVEDVARENDYNVILCSSNNDPV
                     QEAKYLESLERRWVDGIIFSRVTGDTFKEKIPRLLANKVKMVFVDRVPAENRLSIARV
                     EID"
BASE COUNT         1743 a          882 c         1387 g         1633 t
ORIGIN      
        1 tcagtaagat ggagaaggtc ctggatagtt tcttccgttt cactttattt catatggaag
       61 cctgggcaaa gaccagtgcg gaagtaatta ttcagcatga tgacttcgta tggacaggtg
      121 gaccttttat gagaccagag atatacagaa aggtgattat ccccagatat gcagaactct
      181 ggaagccatt gcacagatca gggaaaaagg tcctgttctg ctctgatggt aattttatgg
      241 gatttgcaga ggatatagtt aacgctgggg cagatggact tatctttgag ccctgcaatg
      301 attttggatt tatggttgag agatttggtg gttctactgt actggttgga agctttgttg
      361 actgcagaga cctgaccttt ggaaaatggg aagtggtacg tgccagtata gataaaacag
      421 tcgaactggc taaaaagttc aaaggtatta tgtttgcagt agggaatcat ctcccgccaa
      481 atatacctag tgagatgatg gagagatata taggatatct taaggaggtt ctgtgaataa
      541 gtttagagtt ccatttgaaa aacctgagcc gaatttttct gaatttagag atgttattct
      601 cggtaaaaaa ccagccagga gggttcactt tgcagaatta tttccagata tagagattat
      661 ctggtatatt acagagaata tccttggtga taaggttatc cattatccgc aggatgactt
      721 ccagggtttc tggagacagc agataaattt ctggtacagg atgggatacg actatatcag
      781 agtatctggt ggtttgggtt tcgctgaaca gaaacggagg gttgctgaag atactgcaat
      841 cttacccagg ggacagagga actggataga agagtctgtc gggataataa catcgtggaa
      901 agattttgaa gagtatccct ggccaaaatc agaatcattt gactattcta actacgaatt
      961 tgttttgaaa aatctccctg aagggatgaa gatgctggta tgtccttcga gcggggtttt
     1021 tgagatcagc agtgaatcac ttttaggttt tgaaggtatg agttatctcc tctatgagga
     1081 ttatgagctg gtaaaggcgg tatttgatag ggttggcgag actatttaca ggttttatga
     1141 aaacctcatt acacttgaca atgtggaggg aatctttcag ggagatgacc tcggctttaa
     1201 aaccagcacc atactatctc ctaaatattt aagagcgctg gtcctaccat ggcataaaag
     1261 gtatgctgaa ttggcgcata gatacaacnn nnnnnnnngg cgacagggtg gcagctctgg
     1321 gaggggtgga tgtggataag ctatgcagac tggaggaaag agagctcagg tcttatgtaa
     1381 ggagtatact tgaatgttgt atgcctacaa gatatgcact gggttctgga aattcagtaa
     1441 caaattatgt tcctgttgaa aattatctta ttatgttaga tgaaggggtg aggtactgtg
     1501 attgaattac cattcagaca ggtccatctt gatttccata catccccatt gataccggat
     1561 gttggagtgg attttgaccc tgaagagttt gccaaaacat tgaaagatgc gtatgttaac
     1621 tctataacag tatttgccaa atgtcatcat gggatgtcat attatccgac aaaggttggg
     1681 atggtccatc catcattaaa aagggacctc cttggtgaga tggtagaagc ctgccataga
     1741 gaggggataa gggtatgcgc atatataagt gtggtttggg atgaatattc tgccagtaaa
     1801 catgctgatt ggctccaggt aaataaagat ggaacacttg caggtagacc tccatttggg
     1861 aatagaggat ggagattcct ctgtatgaat tccccatatg tggattatgt ggcagagcag
     1921 acagaggagg tccttaaatc ttatgatgtg gatggggtct tctttgatat agtgatgcag
     1981 acatttccgg ggtgtgtatg taaccattgt atggatagta tgaaaaggct tgggctgaat
     2041 ccggaagatg ataaggatct ctacagacac tctctcatag taagcagaaa ctttatggat
     2101 agaatggata aattagtaaa gagtttaaac tcagatgcga gtatattcta taactccaga
     2161 ctcagacttg aaagcgatga tgagtttggt atgagaccgg aggcggatta ttttacacac
     2221 tttgagatag agtcccttcc ttctggagga tggggatata accatttccc tctctttgta
     2281 cgttactatc agactcttga taaggatttg ctgggtatga caggtagatt tcataagagc
     2341 tgggcagact ttggcggatt taaaaataag cctgcactgg agtatgagtg ttttacaatg
     2401 ctctctctgg gggcgaagtg ttctgttggg gaccagctac acccaagagg gaaactggat
     2461 aagaccgtgt ataatcttat aggtagtgtg tataagaagg tagctgaaag agaagagtgg
     2521 tgcgaagatg caaagcctct atctgatata ggagttctca tctctagttc tttctttaat
     2581 gactggagga ctgactctga tgaaggtgct atgaggatac tgtctgaatt tcactatcaa
     2641 ttctcattta tagattcacg agaagatcta agtaaataca aacttataat agctccagat
     2701 agagtaagta tggatgaagt gctggctgag aaattgagaa aatatctgaa tggtggtgga
     2761 aaacttataa caagttataa gtcagggtta gctggagata agtttattct tgatgatctt
     2821 ggggttgaat atttaggaga tagtgactac tgtcctgatt attttagggt attatcccca
     2881 gcagataagg agattgaacc tgctgagtat gttatgtatg aaagagggac aaaagttcag
     2941 cctatagatg ggactcagat tctggctgag acatatcagc cgtacttcaa caggacctgg
     3001 gagcattttt cctctcatgc tcagacacct ccagataaga agactcctta tccggcagcg
     3061 gttagaaatg ataatgtaat ctacataagc tatcctatct ttagagccta tatattgaat
     3121 ggtaatctgg tttataaaaa acttgtaaga aattgtatag acctgcttct cgataatcct
     3181 ctaactatag ataatgcccc atctacagca aggattactg tcacctcaca gcattacaat
     3241 ggaaaagatc gacatatagt acatatactc cactatattc cagagagaag atgtaaaagt
     3301 atagatgtga tagaagatgt aatccctatc tgcaatatca atctgaaagt gagagttgat
     3361 ggttctcctt ctagatgtta tcttgttcca gagaagaagg aagtaaactt tgtgtataat
     3421 aatggatatg tagagtttac tgtaccggaa gtaaacggtt atcaattgtg gttatagaat
     3481 aaagaaagga gtgaagaaat gagatcaaca ccaataggaa ttgtattgat gaatgatgga
     3541 agacctcatg tgtattcagc caatgaagag gagaatatgc aggtggtaag aaggtgggca
     3601 gaggttataa agaagaatct aaaaaatatt gatggaagct ctccagaagt tgttatcgca
     3661 tctgagacta taaaaagtgt gaggactgcc cagaaaatag cggaagagtt taataaggcg
     3721 aactgtaagc aggttgtaat gtgttataat gtctggaatt tcccctatct ggcatggcct
     3781 tttctaaatt ctctggacgt taatattcct atccttagtc tttcaaataa caatgcacag
     3841 tatccgggga atgttggttt acttgccacc gatggggaga taagacagac gggcaggagg
     3901 acacatagga ttgttggaga tgtggatgat ccaaagattc aggagaaagt gataagatgg
     3961 ttaagagctt ctcaggcagt gaccacaatg agaggagagg tctatggact ttatggtggt
     4021 cactctatgg ggatggagac cggctatttc catcttgtac cgaatataaa gacatttgga
     4081 gttaatacat atcagataga ccagcttttg ctggtaaagg ttatggaaga gatagatgat
     4141 aaagaggtag agaagggatt tcagtggttt acagaactat taggcgatcg tatcctttat
     4201 gataataaaa tgcttacacc agagacgttg aagacgcaga ttagacttta cctggctatg
     4261 aagaaggtta atgaagagaa cggatttgat ttctgtggac ttaagggaca gcgtgaatta
     4321 actgagtatg tatgtctcgg agacatacca gagatgctga tgaatgaccc ctatgactgg
     4381 tatggcttta aggagcctac ggtttgtgcc actgaggctg attcgtttgc agcgttgacg
     4441 atggaggtac ttaaatacat aactggagga cttccggtac tgtttatgga cgtgaggatt
     4501 tattacccag ataaggattt atgggacttc tgtaattctg ggaatcatgc cagttattat
     4561 gcgtctttga gctttgatcc aaaggagaat ttcaagaaga tcacacttta tccagcttta
     4621 ggatactact ttaagacggg aggggcttca gtttcatttg atgctgcacc tacaaagatg
     4681 acattcgcca ggtttggact ctacaatgat aagccatata tggttataat ccctggtgaa
     4741 agtctggacc ttccagagga ggagagaaag gcaataaata aactctctga tcctacctgg
     4801 cctcatgttc atgccagatt gaactgttct ttcgatgagt tcctgaatgt attccctgca
     4861 aaccatatac aggctatacc tggagactat gtggacgctc tcgtatatta ctgtgagata
     4921 cttggtataa agccgatagt tcttggacca aggagtaaag aaagacttac tccaatctgg
     4981 gaaatactgg aataatctct taaaaatcta atcgggggca gagtctctgc cccttttatt
     5041 ttttataaaa cagtaacagt tcatacccct accgggtatt gcattctaga ttatacctgt
     5101 tataataata tcgattttag aaaacgatag taaaaatcga tattagatgg tggagaagaa
     5161 aaatattgat aagaatagtt gatatagccg agaaggcaaa tgtatctcct gggactgttt
     5221 caaagatact taaagggact tatataggtt ctcaagagac aaaagagaga gtgttaaaga
     5281 tagcaaaaga ccttggatac actcccaata taatagccag tagtttaaag agtcgtagga
     5341 ctaggattat tgctttaatt gtcccggaaa tttctgtacc atttttccct gagctaagta
     5401 ggggagtgga ggatgtagcg agagagaatg attacaatgt gatattatgc agcagtaaca
     5461 atgacccagt tcaagaagct aagtatcttg aatccttaga gagaagatgg gtagatggaa
     5521 taatattttc aagagttaca ggagatacat ttaaggaaaa gattcctcgt ctcctggcta
     5581 acaaggtaaa gatggtattt gtagaccgtg ttccagctga gaatagatta tctattgcaa
     5641 gggttgagat agata
//
LOCUS       DAOTKU010000045         5645 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_12957_length_5645_cov_2.901968, whole genome shotgun sequence.
ACCESSION   DAOTKU010000045 DAOTKU010000000
VERSION     DAOTKU010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5645
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12957_length_5645_cov_2.901968"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="P5310_01890"
     CDS             <1..301
                     /locus_tag="P5310_01890"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HRU32150.1"
                     /translation="RFPQVSGLRFTWDPNAEPGKRIVSVEVKNPDGTYQPIDTSKQYR
                     VVTNNFLAQGGDGYTALQKGTNFINLGFVDYEVLREYIVTHSPIDAKVEGRILSK"
     gene            complement(343..1344)
                     /locus_tag="P5310_01895"
     CDS             complement(343..1344)
                     /locus_tag="P5310_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HRU32151.1"
                     /translation="MGKVDINTEEIQNMPWENRPTECTDILWRYSKNPIIKRNLIKRS
                     NSIFNSAVVKFGEKFAGVFRVDDKSRSMNIHSGFSNDGINWTLSEEPIDFIQKTRDPL
                     ISSYKYDPRVTWLEDRYYLTWCNGYYGPTIGVGYTYDFKDFYQIENALLPYNRNGVLF
                     PRKVNGKYAMLSRPSDRGHTPFGDIFYSESPDMIHWGCHRFVMAAGHQYSAWQSLKIG
                     AGPTPIETSEGWLLIYHGVLLSCNGYVYSFGCALLDNEKPWIVKYRTRSYLLSPQEYY
                     ECVGDVPNVAFPCAALCDSQTGRIAIYYGGADTVVCIAFGYVEEIIEYLKSDGIKMV"
     gene            1553..1629
                     /locus_tag="P5310_01900"
     tRNA            1553..1629
                     /locus_tag="P5310_01900"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1587..1589,aa:Cys,seq:gca)
     gene            1636..1710
                     /locus_tag="P5310_01905"
     tRNA            1636..1710
                     /locus_tag="P5310_01905"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1668..1670,aa:Val,seq:gac)
     gene            1733..2389
                     /gene="thyX"
                     /locus_tag="P5310_01910"
     CDS             1733..2389
                     /gene="thyX"
                     /locus_tag="P5310_01910"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HRU32152.1"
                     /translation="MKVELLSYTPEPDRVVAISARLCYSKIGISELAEKLTDDKIKDL
                     LKKLESSGHLSPFEHANFTFGIEGISRVTSHQLVRHRIASYSQQSQRYVKMSNGEIVI
                     PPSIKKNSSASELVYNLNDMAMSVYNKLIQLGIPEEDARYILPQGITTKIIVTMNARE
                     LLHFFNLRCCLRAQWEIRTMANLMLRKVKEVAPIIFEKAGPSCFSGPCPEEGPCPLKK
                     "
     gene            2386..2610
                     /locus_tag="P5310_01915"
     CDS             2386..2610
                     /locus_tag="P5310_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017378947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3006 domain-containing protein"
                     /protein_id="HRU32153.1"
                     /translation="MKFIIDRFEGDIAILVNDITLNLPRSILPEEAREGDVISIEIKI
                     DREETERLRKEVEDKLKSLKGREEKGDIWL"
     gene            2601..3509
                     /locus_tag="P5310_01920"
     CDS             2601..3509
                     /locus_tag="P5310_01920"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HRU32154.1"
                     /translation="MAVKRIRNIIVIISIVVLIIAGLGFNNGLLFNADVRIFVLDVGQ
                     GDSILIKAFDKNILIDAGPDKNLVADRLRLFGVKDLSIIIATHPHSDHIGGMVDVVDR
                     YRPEYYLDPGIPHTSKIYRTLLDKVEEKKLKYIRPGGQTIHIQSAELFIFPVPDGIKN
                     INNGSVVSRLSYKDFSMLFLGDAELEEQAFIMKVNGEKLSSKIMKVSHHGSNNGTDQR
                     LLDAVRPEVSIISVGKDNPYGHPQRETLELMEKNGIKVYRTDRDGAIAILSNGDLYKI
                     IRERGSIIERVMFIILQIKEFISGGI"
     gene            3510..4556
                     /gene="gcvT"
                     /locus_tag="P5310_01925"
     CDS             3510..4556
                     /gene="gcvT"
                     /locus_tag="P5310_01925"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HRU32155.1"
                     /translation="MLITPLYESHKNLNAKFIDFYGWTMPLYYTGVIEEAKATRSNAG
                     VFDISHMARFLITAPSIDFFDRLFTNDPRRLLPGKAHYTLLCREDGGILDDTVLSRLD
                     DNHFLLIVNACNHRKILDWLKEHSIDEEINDVTGELSCIAIQGPRSREIVEKLLRVDL
                     SGLKRFNLVNLNSIIISRTGYTGEDGFEIFAPPQMIMSLWNRSLDLGGIPCGLATRDL
                     LRLEAGYCLYGNELDENRDPITAGLERFVRFDGRKFTGSEKLKKIISDGLKEKLVWFR
                     IQEKVIPRKGDEIRYQGRRIGTVTSGNYSPHFGSGIGMGYVEYNLASAGNSIEIVIRN
                     RSRIGIISRPGYTP"
     gene            4569..>5645
                     /gene="dnaG"
                     /locus_tag="P5310_01930"
     CDS             4569..>5645
                     /gene="dnaG"
                     /locus_tag="P5310_01930"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HRU32156.1"
                     /translation="MSISQRVKERVDIVDLISSYVKLTKSGRNYIGLCPFHDDRNPSF
                     TVSPEKQLFYCFGCKEGGDAIKFLMKIEHLSYREALIQLGKRVGIEIDDSGDESNGGI
                     RELYRTNDLVTRYFHYILMETQYGKTPREYLGKRGIKKDTIETFKLGYAVSSWNALQK
                     FLEKKGVSLETQEMLGLIIRDAETGRIYDRFRDRIIFPIIDITGHTLGFGGRVLDDSL
                     PKYINTPETSIFKKGHILYGLYQTREEIMKKDQAIIVEGYMDCISLYQAGIKNVVASM
                     GTAFTREQGLILKRIAKDIVIAFDADTAGDIAVIRGASVLREVGCNVRIARWTVFKDP
                     DEFIRNQGPIVFLEVINKAKPFLDY"
BASE COUNT         1741 a          961 c         1268 g         1675 t
ORIGIN      
        1 tagatttcct caggtctcag gtttaagatt tacctgggat ccaaatgctg aaccggggaa
       61 gcgtattgta agtgtggagg taaagaaccc tgatgggaca taccagccaa tcgatacatc
      121 taagcagtat agggtggtaa cgaataactt cttagcccag ggaggagatg gctataccgc
      181 ccttcagaag ggaactaatt ttataaatct tggttttgta gactatgaag ttttaaggga
      241 gtacatagta actcattctc ctattgatgc taaggttgag gggcgtatcc tctctaagta
      301 aaaatttaga aaggggctcg gtaggaacga gctcctttat aactagacca tttttatccc
      361 gtcgcttttc agatattcta taatttcctc tacatatcca aaagcgatac ataccacagt
      421 atctgctccc ccataatata tagcgattct tcctgtttga gaatcacaca gtgcagcgca
      481 tggaaatgcc acattgggca catctcctac gcactcatag tattcttgtg gtgaaagtaa
      541 atagcttcta gttctgtact ttactatcca gggtttttca ttatccagca gggcgcatcc
      601 aaaactgtat acatatccat tacaggaaag aaggacccca tgatatataa ggagccatcc
      661 ttcgctggtc tctatagggg ttggacctgc ccctatcttg agggattgcc atgcagaata
      721 ctgatgacct gctgccataa caaatctgtg acaaccccag tggatcatat ctggactctc
      781 actatagaat atatctccaa atggagtatg tcctctatca gatggtctgc ttagcattgc
      841 atatttacca tttacctttc ttgggaaaag taccccattt ctgttataag gaagaagtgc
      901 attttcaatc tggtagaaat ccttaaagtc gtaagtatat cctacaccta tagttggtcc
      961 gtagtatcca ttacaccagg tgaggtagta tctatcttca agccaggtca ctctagggtc
     1021 gtatttataa ctgcttatta gtgggtctct tgttttttgg atgaagtcta tcggttcttc
     1081 gctgagagtc caattgattc catcattgct aaagccacta tgaatattca tagatctgct
     1141 tttgtcgtca actctgaata caccggcgaa tttctctccg aactttacca ctgcactgtt
     1201 aaagatgctg tttgacctct ttataagatt tctttttatg atcggatttt tagagtatct
     1261 ccacaggata tcagtacact ctgtgggtct attttcccat ggcatattct gtatctcctc
     1321 tgtattgatg tctaccttac ccattaaaca cactcctctt tgagagaaga cttacattca
     1381 tagccttcta ctgggctatt tttatcccgc ccttttagaa tagccaccac ccagcaggcg
     1441 gtatttgact acccttggat taattgagat tatactatct gtactgtaag tcttcaacag
     1501 taatatgatg gtggctgttg cttttttaaa atcattatgg tagtatatgt atggggacgt
     1561 agccaagcgg attaaggcag cggattgcaa atccgttatt cctcggttcg aatccgggcg
     1621 tcccctccag agagtgggcg gttagttcag cggaagaacg cttcgttgac accgaagagg
     1681 ccagtggttc aactccacta ccgcccacca ttttttatgc tataatacct gtatgaaggt
     1741 agaactttta tcatataccc ctgagcctga tagagtagtg gcaatttctg caaggctgtg
     1801 ttacagtaag attggtatct ctgagcttgc ggaaaagctt accgatgata agattaaaga
     1861 ccttttaaag aaacttgaat cttcaggaca tctttctcca ttcgaacatg ccaattttac
     1921 atttggaata gaggggattt caagggttac atctcaccag ctggttaggc acaggatagc
     1981 tagctattcc cagcagagtc agagatatgt aaagatgtct aatggagaga ttgttatacc
     2041 tccttctata aagaagaatt cttctgcatc agaacttgtc tataatctta atgacatggc
     2101 tatgtctgta tataataaac tcatacagct aggaatcccg gaggaggatg ccagatatat
     2161 attaccccag ggtataacta ccaagattat tgttactatg aatgccagag aacttctgca
     2221 cttctttaat cttagatgtt gtctcagggc tcagtgggag ataagaacaa tggcaaatct
     2281 tatgctcaga aaagtaaagg aggttgctcc tatcatattt gaaaaagctg gtccgtcttg
     2341 cttcagtgga ccctgtccag aagaaggtcc ctgtcctctc aaaaaatgaa atttattata
     2401 gacaggtttg aaggggatat cgcgatactg gttaatgata taacccttaa tcttccaagg
     2461 tctattcttc ctgaagaagc tagagaagga gatgtcatct ctattgagat aaaaatagat
     2521 agggaagaga cagagagact cagaaaagag gtagaagata agcttaaaag tctgaagggt
     2581 agagaagaga agggggatat atggctgtaa agagaatcag aaatatcata gtcatcatca
     2641 gtatagttgt ccttataata gctgggttag gatttaataa tggattatta tttaatgccg
     2701 atgtaagaat atttgttctt gatgtggggc agggagatag tatcctgata aaggcttttg
     2761 ataaaaatat actcatcgat gctggtcctg ataaaaatct tgttgcagat agattgaggt
     2821 tatttggagt gaaggattta tctattatta tagccacaca tcctcacagt gaccatatag
     2881 gtggaatggt agatgtggtt gatagatata gacccgaata ctatttagat ccaggcatcc
     2941 ctcatacttc aaaaatctat aggacgcttc tggataaggt agaagagaaa aagttaaaat
     3001 acataagacc aggtggtcaa accattcata tccagtctgc agaactcttt atctttccgg
     3061 tacctgatgg tataaagaat ataaataatg ggtctgtcgt aagcaggttg tcttataaag
     3121 atttcagtat gctcttcttg ggtgatgctg agttagaaga acaagccttt attatgaagg
     3181 taaatggaga gaaattaagc agtaagataa tgaaggtctc tcatcatggg tctaataatg
     3241 gaaccgatca gagactcctt gatgcagtaa gacctgaggt atctattatt tctgttggta
     3301 aagataatcc ttatggacat ccacagagag agacattgga actcatggag aaaaacggta
     3361 taaaagtgta caggacggat agggatggag ctattgctat actttctaat ggtgacctct
     3421 acaagattat cagggaacgg ggttctataa ttgagagagt catgtttatt attctccaga
     3481 taaaggagtt tatatcagga gggatataaa tgttaattac tcccctttat gaatctcata
     3541 aaaatttaaa tgctaaattt atagactttt atggatggac tatgcctctc tactatacgg
     3601 gtgtgataga agaggcaaaa gctaccagat ctaatgcagg tgtatttgat atatctcata
     3661 tggcaagatt tttaataact gccccatcta tagatttttt tgatagacta tttacaaatg
     3721 accccagaag acttcttcca ggaaaggcac attataccct gctctgcaga gaagatggtg
     3781 gaatcctgga tgatacagta ctttcaagat tggatgataa tcatttttta ctcatagtta
     3841 acgcctgtaa tcatagaaag atactggatt ggttaaaaga acatagtata gatgaagaga
     3901 taaatgatgt tacaggagaa ctcagttgta tagctattca gggtcctagg tccagagaga
     3961 ttgtcgagaa attgctgagg gtcgatctgt ctggtctgaa aagattcaat cttgttaatt
     4021 tgaacagtat aattatatcc agaacaggtt acactggaga ggatggcttt gagatctttg
     4081 ctcctccaca gatgataatg tccctatgga acaggtcttt agaccttggg gggattccct
     4141 gcgggttagc gaccagagac ctgttgagac tggaggcagg gtactgtctt tatgggaatg
     4201 agctggatga aaatagagac ccaataactg cagggttaga aagatttgtg agatttgatg
     4261 gtagaaaatt tacaggttca gaaaaactca aaaaaattat ttctgatggg ttaaaggaga
     4321 aactggtctg gttcaggatt caggagaaag tcatacccag aaagggtgat gagatcagat
     4381 atcaggggag gaggattggg acggttacat ccggtaatta ttctccccat tttggatccg
     4441 gcataggtat gggttatgtt gaatataacc ttgcttcagc tggaaatagc atagagatag
     4501 ttataagaaa tcggtccaga attgggatta tatcaagacc aggatatact ccgtgatata
     4561 atataaatat gagtatatca caaagggtaa aagagagggt agatatagtt gatcttatat
     4621 cctcctatgt taaattgaca aagtcaggga ggaattatat tggattatgc cctttccacg
     4681 atgaccgtaa tccgtctttt actgtaagcc ctgaaaaaca gcttttttat tgttttggat
     4741 gtaaagaggg cggagatgcg ataaaatttt tgatgaagat agagcatcta tcgtatagag
     4801 aggcgcttat ccagcttgga aagagggttg gtatagagat agatgattca ggggatgaga
     4861 gtaatggtgg aattagagaa ctatacagga ccaatgatct ggtcaccaga tatttccact
     4921 atatacttat ggagacacag tatggtaaga ctccaagaga gtatcttggt aaaagaggga
     4981 taaagaagga tacaatcgag acatttaaac ttggatatgc tgtatccagc tggaatgcat
     5041 tacagaagtt cctggagaag aaaggtgtaa gcctggagac acaggagatg ttaggactta
     5101 ttataagaga cgcagagaca ggtagaatat acgatagatt tagagacagg ataatctttc
     5161 ccattattga cataactgga catactctgg gttttggtgg tagggttttg gatgattctt
     5221 taccgaagta tataaatacc ccggagacat ccatatttaa gaaggggcat atactttatg
     5281 gtttatatca gacaagagaa gagataatga aaaaagatca ggctattata gtcgaagggt
     5341 atatggattg tatatctctc taccaggcag gaataaagaa tgtggtagct tctatgggga
     5401 ctgcttttac cagagaacag gggttaattc tgaagaggat agccaaagat attgtaattg
     5461 cctttgatgc cgataccgcc ggtgatatag cggtaattag aggggcgagt gttttaaggg
     5521 aagtcggctg taatgtacga atagccagat ggacagtatt taaagatccg gatgaattta
     5581 ttcgtaatca gggaccaata gtttttttgg aggtaataaa taaagccaag ccattcctgg
     5641 actat
//
LOCUS       DAOTKU010000046         5577 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_13160_length_5577_cov_3.406918, whole genome shotgun sequence.
ACCESSION   DAOTKU010000046 DAOTKU010000000
VERSION     DAOTKU010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5577
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13160_length_5577_cov_3.406918"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="P5310_01935"
     CDS             <1..337
                     /locus_tag="P5310_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005495906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HRU32157.1"
                     /translation="DLGGKDRGPNPVEYILMAIGGCVTIVGQVIAQEMGIKIESCRIK
                     ASGDLNPLKFMGKPSPDRAGFKGINLEIEVKSDAPREKLEEWIKAIEERCPVGDNLKN
                     PTPVTITLK"
     gene            428..1396
                     /gene="galT"
                     /locus_tag="P5310_01940"
     CDS             428..1396
                     /gene="galT"
                     /locus_tag="P5310_01940"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013430676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HRU32158.1"
                     /translation="MAELRWNPLLNDWVMIASHRQERPQLPKDWCPFCPGSGKVPDHY
                     DVLAYDNDFPALMLIPPEPDDVATDFYRVAPAYGKCEVILYSSDHHTSLPKLSISHIR
                     KLVDLWCERFTELSKHEKIKYVFIFENRGEVVGVTIPHPHGQIYGYSYIPLKILRELE
                     SCKKYYEEKNECLVCRMNMEELSFEKRIIIENEHFLTYLPFFTEYPYGVFIVSRAHKS
                     RIIDFTDEERTSLAEILRDTVGMLDSLFDYEFPYMLCMHQSPVNSEPCDEFYHFHIEF
                     YPPMRSRDKQKFNASSETGAWAPCNTTAPEEKAEELRSAYRRFKKL"
     gene            1393..2562
                     /locus_tag="P5310_01945"
     CDS             1393..2562
                     /locus_tag="P5310_01945"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010502283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HRU32159.1"
                     /translation="MSNIEELKKKFREIFKDSNVRIFHSPGRVNLLGEHTDYNGGYVL
                     PAALNVGTTVCISKRDDKKIVLKATDLDDVVSADIDNLLSYRNIRWGNYQLGVAYELM
                     KAGYKVGGCNMLFDDAVPHGAGLSSSAAIECATGIALISMFNPEREIDRVELALLAQK
                     AENQFVGVNCGIMDQFASIMGKKDNAIFLNCRTLEYKYVPLSLGDYVLVITNTNKKRS
                     LADSKYNERRAECEKGLEFLKKELPYITCLGEITVDIFESIREVIKDPVVEKRVRHVI
                     YEDDRVLKAIEVLSKGNLSEFGKLMIESHISLRDLYEVTGIELDTLFEESLKVKGVLG
                     TRMTGAGFGGCTVSIVHKSAIDDFKRYVGENYRLKTGLTPDFYVMETGEGAREIC"
     gene            2598..2671
                     /locus_tag="P5310_01950"
     tRNA            2598..2671
                     /locus_tag="P5310_01950"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2630..2632,aa:Gln,seq:ctg)
     gene            2690..3112
                     /locus_tag="P5310_01955"
     CDS             2690..3112
                     /locus_tag="P5310_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32160.1"
                     /translation="MNSKKKLKVYLAVPIVNYYNSEVTLLLGRIISEMGFEISSPWVL
                     SGKDAGKTPEDIFYRDIGSVKESDILVAEISSPSHGVGMEIMQAYVSNRKIIMVAREG
                     SNISFLVRGVPGSTIVEYKNYEDLEENLKKILEREMML"
     gene            3109..3966
                     /locus_tag="P5310_01960"
     CDS             3109..3966
                     /locus_tag="P5310_01960"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HRU32161.1"
                     /translation="MKFGVYTPFLPDVFSLLKEVGARSIELACWAGTYTDADVMLTDS
                     ARYEEVISTITSNRLEISALGYYPNNLDPDINRREANNKYLMRLIELADRMGVRVVST
                     FAGRVLDKDIPDNIPIFKEVFTPIIKYAEDKGVKIAIENCPMMHGFPFRGINIAYTPD
                     AWELMFDAIPSPNLGLEYDPSHLYWLQIDYIDAIYNFGDKIFHVHAKDTEIMYNKLAR
                     GGIYSTGWWRYRIPGWGEVDWQKVIAALMDTGYDGGVDIEHEDPVFSGPRTNEGLTLG
                     LKHLAQFIL"
     gene            complement(3956..4831)
                     /gene="hslO"
                     /locus_tag="P5310_01965"
     CDS             complement(3956..4831)
                     /gene="hslO"
                     /locus_tag="P5310_01965"
                     /inference="COORDINATES: protein motif:HMM:NF001033.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HRU32162.1"
                     /translation="MEDYIVKAASDDFSFTVSVAVTTRLVEGAREIHKTSRLATAALG
                     RALSLVGIMGTELEGRQTVSIQVISNGPLKGLFVQSDADGNVRGYIRNPYIELPPSET
                     GDIDVETGIGKSGYLYVVKDMGFGNPYIGITPLVKGGIATDLAHYFYHSEQTPSVVAA
                     GTYIGANGNVEASGGMIGHILPGVSRERLEFFQRRIESLPPLSELIRKSPSIEAILKL
                     IFERIEYTIVDKKIIRYHCPCNKERARTTLILLGKEELYSIMKEGHTEVTCVFCGTNY
                     YFGREELEEIISAIE"
     gene            complement(4834..>5577)
                     /locus_tag="P5310_01970"
     CDS             complement(4834..>5577)
                     /locus_tag="P5310_01970"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HRU32163.1"
                     /translation="VLSGKGGVGKSTVATNLAVGLALNGLDVGLMDIDIHGPNIPKML
                     GIEGSRVQGRDGRIIPVSYGESLKVMSIGFLIPEEDNPVIWRGPLKMNLIRQFVEQTD
                     WGDLDYLVIDSPPGTGDEPLSAVQSFKGLNGAIVVTTPQDVSLLDSKKAINFARALNI
                     PILGIIENMSGFICPHCGEVTEIFKSGGGERISLEMNVPFLGKIPIEPQIVTSSDEGK
                     PYVYFYNRLKSASIFEDIVNRVRATLEEI"
BASE COUNT         1685 a         1021 c         1234 g         1637 t
ORIGIN      
        1 agatcttgga ggaaaagaca gaggtccaaa tcctgttgaa tacatcctta tggctattgg
       61 tggttgtgtt acaatagttg gacaggtgat agcccaggag atgggtataa aaattgaaag
      121 ctgcaggata aaagcatctg gagacttaaa tccattaaaa tttatgggaa aaccaagtcc
      181 tgacagggct ggatttaaag gaataaattt ggagatagag gttaagtctg atgctccaag
      241 ggaaaagctt gaggagtgga taaaggctat agaagaacgt tgcccggtag gggataacct
      301 taagaatcct acacctgtaa ccatcacttt aaaatagatc atctctatta aggaggtggc
      361 ttttaagcca cctccttaac ttttatgcta taatctaaaa gattttacag tggaaggagt
      421 tgatcagatg gcagagttga gatggaatcc gcttctcaat gattgggtga tgatagcgtc
      481 tcacaggcaa gaaagacctc agttacccaa ggactggtgt cctttctgcc cagggtctgg
      541 aaaagttcca gatcattatg atgtgctggc ttatgataat gattttccgg ctcttatgct
      601 aattccccct gaacctgatg atgtggcgac tgatttttac agggtagccc ctgcctatgg
      661 taagtgtgaa gtgatactgt actcttctga ccaccatact tctttaccca agctctccat
      721 ttcacacata aggaaattgg tcgacctctg gtgtgagaga tttacagaac tgagtaaaca
      781 cgagaagata aaatatgtat ttatattcga gaacaggggt gaagtggtag gggtaaccat
      841 acctcacccc catgggcaga tatacggtta ttcatatata cccttaaaga ttttgagaga
      901 actggagagc tgtaaaaaat actatgaaga gaagaatgaa tgtcttgtat gtcggatgaa
      961 tatggaggag ctctctttcg agaaacgtat tattattgag aatgagcatt ttttaacata
     1021 tctaccattc tttactgagt atccctatgg ggtgttcata gtaagcagag ctcataaaag
     1081 caggattata gattttacag atgaagaaag aacctctctg gctgaaatcc tgagagatac
     1141 cgttggaatg cttgacagtc tatttgatta tgaatttccg tatatgctat gtatgcatca
     1201 aagtcctgta aatagtgagc cttgtgatga gttttatcac ttccatatag agttttatcc
     1261 gcctatgcgt tccagagata aacagaagtt taatgcttcc agtgagaccg gagcctgggc
     1321 tccctgcaat acaaccgcac ctgaagaaaa agcagaagaa ttaaggtctg cttacaggag
     1381 gttcaaaaag ttatgagtaa tattgaagag ctaaagaaga aatttagaga gatatttaaa
     1441 gatagtaatg tgagaatctt tcattctccc ggaagggtaa atctccttgg ggaacataca
     1501 gattacaatg gcggatatgt attaccggca gcattaaatg tgggcaccac tgtatgtata
     1561 agcaaaagag atgataaaaa aatagtctta aaagccaccg acctggatga tgtggtaagt
     1621 gcagacattg ataaccttct ctcttacaga aatataagat ggggaaatta tcagctgggt
     1681 gtggcgtatg agctgatgaa agctggttat aaggttggag gttgtaatat gcttttcgac
     1741 gatgctgttc cccacggagc aggactttct tcttctgctg caatagaatg tgctactggt
     1801 atagctctta taagtatgtt caaccctgaa agagagatag atagagttga actggcactt
     1861 ttagctcaaa aggctgaaaa tcagtttgtg ggagtaaact gcgggataat ggaccagttt
     1921 gcctctatta tggggaaaaa ggataatgct atctttttaa actgcaggac actggagtat
     1981 aaatacgtcc ctctaagttt gggggattat gtattggtaa ttaccaatac caataagaag
     2041 agaagtcttg ccgattccaa gtataatgag agaagagctg agtgtgaaaa gggtctggaa
     2101 tttttaaaaa aagaacttcc atatattacc tgtctgggag agataacggt agatatattc
     2161 gaatcgatta gagaggttat aaaagacccg gttgttgaaa agagagtaag acacgtgata
     2221 tatgaagatg acagggtgct gaaggcaata gaagtcctga gtaagggtaa tctatcagaa
     2281 tttgggaaac tgatgataga atcgcacatc tcacttagag acctatatga agttactggt
     2341 atagaactgg acactctatt cgaagaatct ctgaaggtaa aaggtgtgct gggaaccagg
     2401 atgacagggg caggatttgg tggctgtaca gtaagtatag tccacaaatc tgctattgat
     2461 gactttaaga ggtatgtggg agagaactat agattgaaaa ctggtttaac gccggatttc
     2521 tacgtgatgg agaccgggga aggggcaagg gagatatgtt gacttatgtg gtggctatag
     2581 cttataataa aggtaactgg ggaatcgtct aacggtagga caccagactc tggatctggg
     2641 tgtgagggtt cgaatccttc ttccccagcc attttttatg gaggagcagt tgaattcaaa
     2701 gaaaaagctc aaggtatatc tggcagtccc gatagtaaat tactataatt ctgaggtaac
     2761 tttacttctg ggaagaatca tctctgaaat gggatttgaa atctcatctc cctgggtcct
     2821 ttcaggtaaa gatgcgggga aaacacctga agatatcttt tatagagata tcggttcagt
     2881 aaaagaatct gatatactgg tggcagagat atcaagtccc agtcatggtg ttggtatgga
     2941 aattatgcag gcttatgtaa gtaatagaaa gattattatg gtagctagag aaggttctaa
     3001 tatatctttt cttgtaagag gagtgccagg ttcaaccatt gtggaatata aaaactatga
     3061 ggatttagag gagaacctga aaaaaatttt agagagggag atgatgttat gaaatttggt
     3121 gtttacactc catttttacc tgatgtcttc agtcttctaa aagaggtggg tgcaagaagt
     3181 atcgaactag cctgctgggc aggaacttat accgatgctg atgtgatgct tacagatagt
     3241 gctcgatatg aagaggttat atctactatc acatctaaca ggcttgagat ctctgccctg
     3301 ggctattatc caaataatct ggaccctgat ataaacagga gagaagcgaa taataaatat
     3361 ctgatgaggc ttatagaact tgcagataga atgggagtaa gagtagtaag tacgtttgct
     3421 gggagagttc tggataagga catccctgat aatattccaa tctttaagga agtctttact
     3481 ccaataataa aatacgccga ggataaaggg gtaaagattg caatagagaa ctgccccatg
     3541 atgcatggat ttccattcag agggattaat atagcatata cgcctgatgc ctgggagttg
     3601 atgtttgatg ctatcccttc tcccaatctt ggacttgagt atgatccgag tcatctatac
     3661 tggcttcaga tagattatat agatgccata tataactttg gggataaaat ctttcatgtt
     3721 catgctaagg atacagagat tatgtacaat aaactggcta gaggcggaat ctatagcacc
     3781 ggctggtggc gttacaggat tccgggttgg ggagaggtgg attggcagaa agttatagct
     3841 gcacttatgg acacaggtta tgatggaggt gtagatatag agcatgaaga cccggtattt
     3901 tctggaccaa gaaccaatga agggttaact ttaggattga agcatctcgc ccagtttatt
     3961 ctatagctga tattatttct tccagctctt ctctgccgaa atagtaatta gttccacaga
     4021 acacgcaggt tacttctgta tgtccttcct tcatgatcga gtaaagttct tcctttccaa
     4081 ggagtattag ggtggttctg gctctttcct tattgcaagg acaatggtat cttataattt
     4141 tcttgtcaac tattgtgtat tctattcttt caaatataag cttcagtata gcctctatcg
     4201 atggagactt tcttataagt tcactcagag ggggaaggga ttcaatcctc ctttggaaaa
     4261 actctaatct ttcacgggat actccgggca gtatatgtcc aatcattcca ccggatgcct
     4321 caacatttcc attagctcct atatatgtcc cagcagccac aacagacggg gtctgctcag
     4381 agtgatagaa gtaatgggca aggtctgttg ctattcctcc ttttacaagt ggagtaattc
     4441 ctatataagg attaccgaaa cccatatctt tcaccacata taaataccct gattttccaa
     4501 tccctgtttc cacgtctata tctccagtct cactgggagg tagttctata tatggatttc
     4561 ttatatatcc ccttacattt ccatctgcat cagactggac aaacaacccc tttagaggtc
     4621 catttgatat aacctgaata cttaccgtct gtcgtccctc aagctctgtc cccattattc
     4681 caacgagact cagggctcta ccaagagcag cggtagccag tctgctggtt ttgtgaatct
     4741 ctctcgctcc ttcaacaagc ctcgttgtaa ctgctacgct tactgtaaaa gaaaaatcat
     4801 ctgatgctgc ctttacaata taatcttcca tatctatatc tcctctaaag tagctcttac
     4861 cctgttgact atatcttcaa atatactggc tgattttagt ctgttataaa aatatacata
     4921 aggtttacct tcatcagaag atgttactat ctgaggttct attgggatct ttccgaggaa
     4981 gggaacgttc atctccagag atatcctttc tcctccacca gatttaaata tctctgtaac
     5041 ttctccacaa tgagggcata taaatccact catattctct ataattccaa ggattgggat
     5101 gtttaatgct ctggcaaagt ttatagcctt ttttgaatct aaaagagata catcttgagg
     5161 ggtggtaact actatagccc cattcaagcc tttaaatgac tgtactgcag ataaaggttc
     5221 atctccagtc cctggcggag aatctattac cagataatcc agatcacccc agtctgtctg
     5281 ttctacaaat tgccttataa gattcatctt caggggacct ctccagatta ccggattatc
     5341 ttcctcaggg atgagaaaac cgatagacat aacttttaaa ctttctccat aagagacagg
     5401 aataattctt ccatctctac cctgcaccct ggatccttct attcccagca tcttggggat
     5461 attgggtcca tgaatatcta tatccataag tccaacgtcc agtccattca atgctaatcc
     5521 aaccgctaaa ttggtggcta cagtactttt gcctacccca cccttaccac ttaatac
//
LOCUS       DAOTKU010000047         5508 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_13329_length_5508_cov_3.559142, whole genome shotgun sequence.
ACCESSION   DAOTKU010000047 DAOTKU010000000
VERSION     DAOTKU010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5508
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13329_length_5508_cov_3.559142"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="P5310_01975"
     CDS             <1..326
                     /locus_tag="P5310_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32164.1"
                     /translation="VRFNREFATVDKWDAARILAEEGLPVGAHYVVPIYNQYWIRNRV
                     TYGNSQYPWSMPGAREIDYTNCCPDAERALEDHMTLYLHEGWGEREIDYTIESFKKVE
                     KEYSV"
     gene            338..1372
                     /locus_tag="P5310_01980"
     CDS             338..1372
                     /locus_tag="P5310_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019421047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRU32165.1"
                     /translation="MEDRVLNFAIIGCGVIAPNHAEGIVNTPSARLVAVADIVEEKAV
                     KFAQKYNCDWYTDYKKMLDRPDIDVVNICTPSGLHPEMAIEAMKRGKHVITEKPMAIT
                     LEQADAMISASKEYKRKLACIFQRRTQSIFKKIKEVTSQGAIGKLILGDAYMKYFRSQ
                     AYYDSGDWRGTWALDGGGALMNQGIHCIDLMQWIMGEVGSVFAYTRTLARKIEVEDTA
                     VSVLEYKNGAIGIIEGTTSVVPGFDHRLEFHGVDGSILVNGEKIARWEVPGLELKLDS
                     YETAGSTASDPRVASYRGHQILIQDMIDAIKNDRDPLIPGEEGKKALKIILAIYKSSR
                     ERKEVYLDTM"
     gene            complement(1594..2460)
                     /locus_tag="P5310_01985"
     CDS             complement(1594..2460)
                     /locus_tag="P5310_01985"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HRU32166.1"
                     /translation="MIIDIHTHLGRVTREDIPVTAEELIENMDKWEIEKAVVLPLDAT
                     PEGSTFWFTTEQVLEVYRRYPDRIIPFCKLDPRQIDNSMKTDFSWILQEYKDKGCRGV
                     GEITANLYIDDPMGMNLFAQCGEANMPVLFHLVDRIGAQYGLVDNIYLPRLERILREL
                     PETTFIGHAMSFWAEISTDVTEETRGGYPKGLIKSPGRLQELLRKYDNLYGDLSAGSG
                     FNAITRDPEYGYKFLEEFQDKLLFGTDFCHHNQEVPIVDYIKSVLNDGKISRTTYEKI
                     THINAEKLLKMK"
     gene            complement(2494..3264)
                     /locus_tag="P5310_01990"
     CDS             complement(2494..3264)
                     /locus_tag="P5310_01990"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRU32167.1"
                     /translation="MNIIDIHVHPELEDQDYSLTEKMIGLAKRYGVKHICLLGDVLHF
                     GYDPSEEQIRRINDQTIELVEKWSDFFFGFCFLNPKHPEEFMQQEAGRCFKNKNFKGI
                     KLEVAVNARNKHVEKVMRIARNMNIVVLLHSWSTEIIDNREHQSDPEDIAYLASHFPD
                     VKIIMAHVTSARERGVLAIKPFSNVWVDTSGGQPIAGTIEYAVEKLGSERILFGSDAP
                     GRDFSAQLGKIYGSNISEYAKRSILGENARKLLGITDG"
     gene            complement(3261..4037)
                     /locus_tag="P5310_01995"
     CDS             complement(3261..4037)
                     /locus_tag="P5310_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32168.1"
                     /translation="MPDKETCPIFDINVHLGCWHNQRLPYTSPLQLQKKLNSLGIRKA
                     FVSSNQAIFYSDPEPGNLELLNKVKRYPLFVPVPVFNPNIPNSEEILRKYSKLKIKII
                     KLFPSFHNYSLNSEKIKSVLKFCLLEGFIILIQMRMEDERLQHPTLKTNPLDIDKILE
                     IAEMYPSLPIICLSGYFNECVRLAQNSSNIYLDISFAERLDTIKALLDVIPLSRIVFG
                     SHTPFFYPESALLKVESSEIPLCWKEHILYKNIETLMEER"
     gene            complement(4027..5250)
                     /locus_tag="P5310_02000"
     CDS             complement(4027..5250)
                     /locus_tag="P5310_02000"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRU32169.1"
                     /translation="MRQEGWRQYKESLKYLASGSSTSSKIPIYENIEPPLIVKGKGCR
                     VWDVDGNEYIDFRNALGPVSIGYSIDQINSQIKEQLDNGIIFGHPSPIEGEVAKLLVE
                     NIPCAEKVRFLKTGGEAISACIKIARAFTGKNKIVHCGYNGWLNNLALGEGMPPGISK
                     SQPLRGVPQQILSLHFTLPWAVIERWIKLFEKEGNDIAAVVVASAYQDMEKGEDFLPQ
                     VRELTRRYDSLMIMDEIVTGFRLAIGGAHQYFNFLPDMAVFGKAMANGMPISAYLGKS
                     ELIELAREIGISSTFGGETLSLASAKATIRFYTENEVTSHLWQMGNLLKDGINEIFKR
                     YNFPCELKGFGPCPQFVFKDDLMKDRFFQGCYRNGVSFYHVVYVNYSHKEKDIEETLE
                     RVEETIRELKDEDAR"
     gene            complement(5263..>5508)
                     /locus_tag="P5310_02005"
     CDS             complement(5263..>5508)
                     /locus_tag="P5310_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32170.1"
                     /translation="IILAEASRYAGIVTREIDLEQKRLSNFHFADGDYNRTLAILSSR
                     RPELYGELINPASKERALRSIRETPKNSGCGDKQNYW"
BASE COUNT         1588 a         1102 c         1099 g         1719 t
ORIGIN      
        1 ttgtaagatt taatagagaa tttgcaactg tagataagtg ggatgctgcc agaatattag
       61 ctgaggaagg tttaccggta ggggcacatt atgtcgttcc tatctataac cagtactgga
      121 taaggaacag ggttacctat ggaaattctc agtatccctg gagtatgccc ggggcaagag
      181 agatagatta cactaattgc tgtcctgatg ctgagagagc actggaagat catatgacgc
      241 tgtacctgca cgaagggtgg ggagaaagag agatagatta tacgattgaa tcgtttaaga
      301 aggtagagaa agaatactct gtataggagg ctatcatatg gaagatagag ttttgaattt
      361 tgccattata ggttgtgggg ttatcgctcc aaatcatgca gaggggatag ttaatacccc
      421 ttcggccaga ctggtagcgg tggcagacat agttgaagaa aaggcggtaa aatttgccca
      481 gaagtataac tgtgactggt atacagatta taaaaagatg ctggatagac cggatataga
      541 tgtggttaac atatgcactc ccagtggatt acacccggaa atggctatag aggctatgaa
      601 gagaggtaaa catgttatta cagaaaaacc tatggctata actttagaac aggcggatgc
      661 aatgatatca gccagtaaag agtataaaag aaagctagcc tgtatctttc agagaaggac
      721 tcagagtatc tttaagaaga taaaagaggt aacttcgcag ggagctatag ggaagctcat
      781 ccttggagat gcatatatga aatattttag aagtcaagcc tactatgaca gtggagactg
      841 gcgtggaacc tgggctctgg acgggggagg agccctgatg aaccagggga tacactgcat
      901 agaccttatg caatggatta tgggagaagt gggttctgtc tttgcatata ccaggacatt
      961 ggcgaggaag attgaagttg aagatactgc cgtatctgtt ttagagtata agaacggagc
     1021 gataggtatt atagaaggga caacatctgt agtgcctggc tttgatcaca gattggaatt
     1081 tcatggggta gacggcagca ttcttgtgaa tggagaaaag attgcaagat gggaagtccc
     1141 aggattggag ctcaagttag attcttacga gactgcaggt agtaccgcat ccgatccaag
     1201 agtagcgagt tatcgtgggc atcagatttt aatacaggat atgatagatg cgatcaaaaa
     1261 tgatagagac cccctgatcc ctggagaaga ggggaagaag gcgttaaaga ttatacttgc
     1321 catatataaa tcaagtagag agagaaaaga ggtatatctc gatacaatgt aataactaaa
     1381 cattatcctt ttgtataatt cagatcctga tttaaccggt aaaaatttaa gtaagaatat
     1441 cttctatttt gaagatatgg aatatttccc tttattgcta aaaaggaaaa ttgtattttc
     1501 caatattggg attgaggcag aagagaagct tggtcaggtt atagatggta gcttcagctg
     1561 ggatattcat ctgaagatat acggcgagaa ttcctatttc attttaagaa gtttttcagc
     1621 atttatatgt gttattttct cataagtggt tctcgatatc tttccatcgt taagaacaga
     1681 ttttatataa tctactatgg gaacctcctg attgtgatgg cagaagtctg tgccgaataa
     1741 aagtttatcc tgaaactcct ctaaaaactt atacccatat tccgggtctc tcgtaatagc
     1801 attaaatcca ctacctgctg ataggtctcc atagagatta tcatatttcc tcaacagttc
     1861 ctgtaatctc ccggggcttt taatcagtcc tttcggataa cctccccgtg tctcttctgt
     1921 tacatcagta cttatctctg cccaaaaaga catagcatgt ccaataaaag tagtttcagg
     1981 aagttctctc agaatccttt ctaatcttgg taaataaata ttatcaacga gcccgtattg
     2041 agcacctatt cggtccacta ggtgaaacag cacaggcata ttagcctcac cacactgggc
     2101 aaagaggttc attcccatgg ggtcatctat ataaagattt gcagttatct cacctacacc
     2161 tctgcatcct ttgtccttat actcctggag aatccaagaa aagtcagttt tcatggagtt
     2221 atctatctgc cttggatcaa gtttacagaa tgggattatt ctatctggat atctcctgta
     2281 aacctccaaa acctgttcag tggtaaacca gaatgtgctc ccctcagggg tggcatctaa
     2341 aggtaacact accgccttct ctatctccca tttatccata ttttctatca attcctctgc
     2401 cgttaccggg atatcttccc ttgttactct ccccagatgt gtatggatat caataatcat
     2461 agcttattca tcctccaaat taatttatag ggactagcca tcagttatcc ctaaaagttt
     2521 ccgtgcattt tcccctagga ttgatctttt cgcatattct gaaatattcg aaccataaat
     2581 ctttcctagc tgtgctgaaa aatccctccc aggagcatca gaaccaaaaa gtatcctctc
     2641 agatccgagc ttttcaactg catactcgat agttcctgca ataggctgtc ctccagatgt
     2701 atctacccag acattcgaga atggctttat ggcaagcacg ccacgctctc ttgctgatgt
     2761 cacatgtgcc ataataattt ttacatcagg aaaatgggaa gctaggtagg ctatatcctc
     2821 agggtcagat tgatgttccc tattatcaat gatctctgta ctccaggaat ggagaagtac
     2881 aacaatattc atatttcgtg caatacgcat gaccttttcc acgtgtttat tcctggcatt
     2941 tactgcaacc tccagtttta tccccttaaa gtttttgttt ttgaaacagc gtcctgcctc
     3001 ttgttgcata aactcttctg gatgtttggg atttagaaag cagaatccaa agaagaaatc
     3061 tgaccatttc tcaactaact ctattgtctg gtcattaatt cttcttatct gctcttctga
     3121 tggatcataa ccgaagtgta ggacatcccc tagcagacat atatgtttca ctccatacct
     3181 ttttgctaac cctatcattt tttctgttaa actgtaatct tgatcctcaa gttcaggatg
     3241 aacatgaatg tctataatat tcatctttcc tccataaggg tttcaatgtt tttgtaaaga
     3301 atatgttcct tccagcaaag aggaatctct gaagattcta cctttaataa cgctgattca
     3361 ggatagaaaa atggcgtatg tgagccgaaa actatccttg aaagaggaat aacatcgaga
     3421 agagctttta tagtatccaa tctttctgcg aatgaaatgt cgaggtaaat attggaggaa
     3481 ttttgggcta accttacaca ttcgttaaag tagcctgaaa gacagattat aggaagggag
     3541 ggatacattt ctgcaatctc taaaatttta tctatatcta acgggttggt ctttaaagta
     3601 ggatgctgta gcctttcatc ttccatcctc atctggatta aaattataaa gccttcaagg
     3661 agacagaatt ttaatacaga tttaattttt tcagaattaa gggaatagtt gtggaaggat
     3721 ggaaagagtt ttattatttt tattttcagc tttgaatatt ttctcaatat ctcctctgaa
     3781 ttcggtatat tagggttgaa gacagggaca gggacgaaca gaggatatct ttttacttta
     3841 tttaaaagct ctaagttacc tggttcaggg tcactgtaaa aaatagcttg attgcttgag
     3901 acaaaggctt ttctaatccc taaagagttc agttttttct gcagctggag tggtgatgta
     3961 taaggtagcc tctggttatg ccaacacccc agatgcacat ttatatcgaa tattggacaa
     4021 gtttctttat ctggcatctt catctttcaa ctcccttata gtctcctcaa ctctttctag
     4081 cgtctcttct atgtcctttt ctttgtgtga atagttaaca taaacgacat gataaaaaga
     4141 aaccccattt ctatagcacc cctgaaagaa cctatctttc attaaatcgt ctttgaaaac
     4201 aaattgtgga cagggaccga aacctttcag ctcacagggg aaattatatc ttttgaaaat
     4261 ctcgtttatg ccatctttta aaagattccc catctgccat aaatgggagg ttacctcatt
     4321 ttcagtgtaa aaccttattg ttgcttttgc ggaggcgagg gagagtgttt ctcctccaaa
     4381 ggttgaggag attccaattt cccttgccag ttctattaat tctgactttc caagatatgc
     4441 agatattggc atgccatttg ccattgcctt cccaaacaca gccatatcag gcaagaagtt
     4501 aaaatattga tgcgctcctc caatcgccag tctgaaccct gttactattt catccattat
     4561 catcaggctg tcatatcttc tcgttaactc tcttacctgc ggcagaaaat cttctccttt
     4621 ttccatatcc tggtatgctg atgcaacgac aactgctgca atgtcatttc cctccttctc
     4681 gaaaagtttt atccatctct ctattactgc ccacggaagt gtaaaatgga gggacaagat
     4741 ttgctgagga actcctctta atggctgact ttttgatatc cctgggggca tcccttctcc
     4801 taaggcaaga ttgtttagcc atccattata accacagtga actattttat ttttccctgt
     4861 gaaggcacga gctatcttga tacaggcaga gatagcttct ccacctgtct ttaaaaatct
     4921 aactttttcg gcacagggga tattctcaac aagtagtttt gcaacctctc cctcaatggg
     4981 acttggatgc ccaaagatta ttccattatc aagttgctct tttatttggc tgtttatctg
     5041 atctatcgag taacctatag aaacaggacc aagtgcattt ctgaaatcaa tatattcgtt
     5101 tccatctaca tcccagactc tgcatccctt acccttgaca attaaaggtg gctcgatgtt
     5161 ttcataaata ggtatctttg aagatgtgga gctaccactt gctaggtatt ttaagctttc
     5221 tttatattgt ctccaacctt cttgtctcac ttttttagcc tcctaccaat aattttgctt
     5281 gtctccacaa ccagaatttt ttggagtctc cctgatggac ctcagggctc tttccttgga
     5341 agccgggttt attagttccc cgtaaagttc tggtctgcga ctggacagta tagcaagggt
     5401 cctgttatag tctccatcgg cgaaatgaaa gttggagagc cttttctgtt ccaaatcgat
     5461 ctcacgcgtg acgatacctg catacctaga agcttcagcc agtattat
//
LOCUS       DAOTKU010000048         5407 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_13602_length_5407_cov_3.027280, whole genome shotgun sequence.
ACCESSION   DAOTKU010000048 DAOTKU010000000
VERSION     DAOTKU010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5407
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13602_length_5407_cov_3.027280"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..965)
                     /gene="tyrS"
                     /locus_tag="P5310_02010"
     CDS             complement(<1..965)
                     /gene="tyrS"
                     /locus_tag="P5310_02010"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HRU32171.1"
                     /translation="MSEDDLEKLLRNTVQVISVEDLRERLEESERLKRPLRVKLGVDP
                     TAPDLHLGHYVGLRKLREFQKFGHIIVFVIGDFTARIGDPSGRIETRKSLSEEDVKAN
                     AETYAKQVFKVLDREKMELRFNSEWLGKLSSYDIIRLTSNYTVARMLERDDFQKRFSE
                     GRPISIHEFLYPLFQAYDSVAIRADIETGGTDQTFNLLVGREIQPVYGQKPQIVLTWP
                     IIEGLDGVVKMSKSYGNYVALEDPPMEMYGKLMSIPDHLIIKYLTLLTDFSDQDIRRW
                     GKEMENGYNPKLVKEILAIEIVSTFYSRDIALECKEEFNRIFSQR"
     gene            complement(958..1617)
                     /gene="phoU"
                     /locus_tag="P5310_02015"
     CDS             complement(958..1617)
                     /gene="phoU"
                     /locus_tag="P5310_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HRU32172.1"
                     /translation="MNPRHIFDEEIRRLRTDLLRMGTLAREAVDKSIKALVNRDSSLA
                     EEVIVGDDIIDKMDLDIEMKCMELIARQQPMARDLRVIGTILKTITDLERIADHAVDI
                     AKIVKILADQPPLKPFIDIPRMCEISLSMLDDALKAFVNEDTELARKMCERDDLVDAL
                     YDQVFRELLTYMMEDPKAVVRALYLIFIARFLERVADHATNVGERVVYMITGVLKELN
                     V"
     gene            complement(1635..2417)
                     /gene="pstB"
                     /locus_tag="P5310_02020"
     CDS             complement(1635..2417)
                     /gene="pstB"
                     /locus_tag="P5310_02020"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HRU32173.1"
                     /translation="MNNKLKIKISVRNLNIYYGKFLAIKNITLDIPERSITAVIGPSG
                     CGKSTFLRALNRMNELLPDVKTEGEVSIDGRNIYSPDVDLVKLRRNVGMVFQRPNPFP
                     KSIFENVAFGLRIQGIRDRERIEETVELSLRRAALWDEVKDKLYKSALELSGGQQQRL
                     CIARALAVQPQVILMDEPASALDPIATLRIEDLMRELKEEYTIVIVTHNMQQAARVSD
                     FTAFFLTDEDRAGMLIEYGNTSDIFTMPKDPRTEGYITGRYG"
     gene            complement(2420..3262)
                     /gene="pstA"
                     /locus_tag="P5310_02025"
     CDS             complement(2420..3262)
                     /gene="pstA"
                     /locus_tag="P5310_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HRU32174.1"
                     /translation="MRFKEKIYLSLTALATLIIIGLLSYIIFDILKNGIGVITWEFLS
                     QPPREGMTKGGIFPAIYGTLVRTLLMLVFVVPIGVFTAIYLNEYGRGSNFSKWIRLAI
                     NNLASVPSIVFGLFGMGFFVLFLRLGAGLLSAALTIGILTLPIVVVSTEEALRAVPNS
                     YREAGFALGATKWQVIRSIVLPQALPGILTGAILSVGRGMGEVAPLLFTGVAYYLPYL
                     PSSIFDQFMDLGYHIYIMTTQSFNIELTRPIQYGTALVLLMLTLSFNIIAIFIRYRMR
                     RKRS"
     gene            complement(3259..4158)
                     /gene="pstC"
                     /locus_tag="P5310_02030"
     CDS             complement(3259..4158)
                     /gene="pstC"
                     /locus_tag="P5310_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002615976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HRU32175.1"
                     /translation="MEVREKLAYIIISLLASVAIISVILIFIFIARESLPIFFNPEIK
                     KEANLRVFFFTRDWQPISLIPKYSFIPLIVGTVKVTIVAIVIAFPLSLGAALFTSEFI
                     PGKFREIIKPVIELFSAIPSVVLGFFALMNIATWVQVLFHASSRLNAVTAGFALAFAV
                     IPITYTICEDALNTVPRELREASIALGATKWQTAVKVVLPAASGGVFAGIMLGLARAM
                     GETMIVLMVSGNAAQITLSFLDSVRTIPATIAAEMGEVAFGSAHYATLFFLGLLLFSI
                     TFVINYIASTLLERTQKRLRGER"
     gene            complement(4226..5167)
                     /locus_tag="P5310_02035"
     CDS             complement(4226..5167)
                     /locus_tag="P5310_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aryldialkylphosphatase"
                     /protein_id="HRU32176.1"
                     /translation="MIVTVRGPLDSSSLGRTLVHEHVLVDFSAMDITSRDKYDTDDVI
                     EKVTPFLEDLQRFGFTGFVDCTPSYLGRDIRLLHKLSERLDMHIITNTGYYGAANDRY
                     VPKFAYEEREEKLANRWIKEYRDGIEDTGIRPGFIKIGVDPGPLSEIDRKLVSASAMT
                     HLETGLTIACHTGEGEAALDVLNVIKSYKVSPSALIIVHADSIKDRDVIFKLADEGCW
                     VELDSVGSKPIETHLELIGEVIKRNFISQLLISQDAGWYRVEEPDGGMNKFNPYIHIP
                     EILIPALVRDISEDIINIIFIENPTRALSISGRKLCS"
BASE COUNT         1694 a         1233 c          847 g         1633 t
ORIGIN      
        1 tctctctgtg aaaatatcct gttaaactct tccttacatt ccaaggctat atctctacta
       61 taaaaagtag atacaatctc tatcgccaga atctctttca caagcttagg attataacca
      121 ttctccatct cttttcccca gcgtcttata tcctgatctg agaaatctgt gagaagggta
      181 agatatttaa ttattaaatg gtccggaata gacataagtt tcccgtacat ctccatagga
      241 gggtcttcaa gtgccacata attcccataa cttttactca tctttaccac accatctaac
      301 ccttctatta taggccaggt aaggactatt tgaggttttt gaccatagac aggctgaatt
      361 tctctaccaa ccaaaagatt gaatgtttga tccgttcctc cagtctctat gtcagctctt
      421 atcgctactg aatcataagc ttgaaatagg ggatacagaa attcatgtat acttatagga
      481 cgtccttcac taaacctctt ctgaaaatca tccctctcta acatcctggc tactgtatag
      541 ttactggtaa gccttataat atcataagag gatagtttac caagccattc actgttaaat
      601 cttaactcca tcttctccct gtccaaaact ttaaaaacct gcttggcgta tgtttctgca
      661 ttggctttaa catcctcctc tgaaagagat tttctagttt ctatcctgcc agaaggatcc
      721 ccaatccttg cggtgaaatc tcctataaca aaaactatta tgtgcccaaa tttttgaaat
      781 tctctgagct ttctcagacc cacatagtgc cctaaatgca agtctggagc tgttggatct
      841 acccccagtt tcaccctgag aggtctcttt aatctctcag actcttccaa cctctctctt
      901 aaatcttcta cagaaataac ctgtactgta ttcctcaaaa gtttttctaa gtcatcttca
      961 gacatttagc tcttttaaca ccccagtaat catataaact actctctctc caacattagt
     1021 tgcgtgatct gctactctct ctaaaaacct ggcaataaat ataagataca atgccctcac
     1081 aactgcctta ggatcctcca tcatatatgt caggagttct ctaaaaacct gatcatacaa
     1141 agcatctacc aggtcatctc tctcacacat cttcctggct aattcagtat cttcgtttac
     1201 aaaggctttt aaggcatcat caagcatcga taaagagatt tcgcacattc ttggtatatc
     1261 tataaatggc tttaagggag gttgatctgc aagtatcttg actatcttcg caatatcaac
     1321 cgcatgatct gcgattctct ccagatcagt aatagtcttt aatatggttc ctataactct
     1381 caagtctcta gccattggtt gttgtctagc tataagttcc atgcatttca tctctatatc
     1441 cagatccatt ttatctatta tatcatcacc aactattact tcttctgcaa gagaagaatc
     1501 tctatttaca agagccttga tagatttatc tacagcctct cgagctaaag ttcccattct
     1561 caaaaggtct gtccttaacc gtctgatctc ctcatcaaat atatgtcttg gattcaccat
     1621 aatccatcat ccttctatcc atatctccca gttatatatc cttccgttct ggggtccttt
     1681 ggcatagtaa atatatccga agtattccca tactctatta gcataccagc acgatcttca
     1741 tctgtcaaga aaaatgctgt aaagtcagaa actcttgccg cctgttgcat attatgtgtc
     1801 acaataacta tagtatactc ctctttcaac tccctcataa gatcttctat ccggagggtt
     1861 gctatgggat caagagcaga agccggctca tccatcagta ttacctgtgg ctgaacagca
     1921 agagccctag ctatacacaa cctctgctgt tgtcctccag aaagctccag ggcagattta
     1981 taaagtttat cttttacctc atcccagaga gcagcccttc tcaaactcag ctctaccgtt
     2041 tcttctatcc tctctctatc tctgataccc tgaatacgaa gaccaaaagc cacattttca
     2101 aatatagatt tagggaatgg gttcggtctc tgaaaaacca tgccaacatt tctccttaat
     2161 tttaccagat cgacatctgg tgaatatata ttccgaccat ctatcgagac ttctccctca
     2221 gttttaacat ccggaagaag ctcattcatc ctgtttaaag ccctcaggaa tgttgatttt
     2281 ccacaaccag atggaccgat aacggcagtg atactccttt ccggaatatc cagggttata
     2341 ttctttatag ctaaaaattt tccataatat atatttaaat tcctcactga aatttttatt
     2401 tttaacttat tattcattat caggacctct tcctcctcat tctatatctt ataaatatag
     2461 cgattatatt aaaagaaagc gtaagcataa ggagaactaa tgcggtacca tactgaatag
     2521 gtcttgtcaa ctctatatta aaggattgag tagtcattat gtatatatga tatcctaaat
     2581 ccataaattg gtcaaaaata gaagatggca gatagggaag atagtatgca actcctgtaa
     2641 ataaaagagg agcaacctct cccatccccc tacccacgct caatattgct ccagtcaata
     2701 tccctgggag agcctggggt aaaacgattg atcttataac ctgccattta gtagctccaa
     2761 gagcaaaacc agcctctcta taggaattag gaacagccct taaagcctct tcagtggaaa
     2821 ccacaactat aggaagggta agaataccta tagttaaagc agcggataga agacctgctc
     2881 caagcctcag aaacaggaca aaaaacccca taccgaaaag accaaagact atcgagggaa
     2941 cactggcaag gttatttatc gccagtctaa tccacttaga aaaatttgag cctctgccat
     3001 actcattaag atatatagcg gtaaataccc ctattggtac aacaaatacc agcatcaaca
     3061 gggtcctgac taaagtgcca tatatggcag ggaatatacc cccttttgtc ataccctctc
     3121 ttggtggctg agagagaaat tcccaggtta ttacacctat tccgtttttt aatatgtcaa
     3181 agataatata agagagtaag cctataatta ttaatgttgc cagagcagtg agggataaat
     3241 aaatcttctc cttaaacctc atctctcacc tctaagtcgt ttctgagttc tctccaggag
     3301 ggtggaggct atataattta taacaaaggt gatagagaat agaagtaatc ctaaaaagaa
     3361 gagggttgca taatgagcac ttccaaatgc tacttcaccc atctctgcag ctatagttgc
     3421 agggatagtt cttacagaat ccagaaaact caatgttatc tgtgcagcat tcccggaaac
     3481 cataaggact atcatggtct cacccatagc cctggctaac ccgagcatta tccctgcaaa
     3541 aacacctcca gatgcagcag gtaaaaccac cttaaccgca gtctgccact tagtagcccc
     3601 taaagcaata ctggcttctc tcaactctcg aggaacagta ttaagtgcat cctcacatat
     3661 agtataggta atcggtataa cggcaaatgc aagggcaaaa cctgcagtta ctgcatttaa
     3721 tcttgaagat gcatgaaata aaacctgaac ccaagtggct atattcataa gggcaaagaa
     3781 accgagaact accgaaggga tggctgagaa cagttctatc actggtttta ttatctctct
     3841 aaattttccg ggtataaatt cagaggtaaa aagagcagcc cctaaagaga gaggaaatgc
     3901 gataacaatc gcaactatcg taactttaac agtacctact ataagaggga taaaagaata
     3961 tttaggtatt aagcttatag gttgccagtc tctggtaaag aagaaaaccc ttaaatttgc
     4021 ctctttttta atctccggat taaagaaaat gggtaaagat tctcttgcta taaatataaa
     4081 tattaaaata acacttataa tggcaacact agctaataaa gatatgatta tataagcaag
     4141 tttttctcta acttccatac tctaatttta taccataata aaatagtata gcaataataa
     4201 accggttcat ttttttaaga aattattaag agcataactt tctaccagat atagaaagtg
     4261 ccctggtcgg gttttcaata aatattatat tgattatatc ctcacttata tctctaacca
     4321 atgcaggtat aagaatttca gggatatgaa tatatggatt aaatttattc atgccaccat
     4381 ctggctcttc aactctgtac catccagcat cctgagaaat cagaagttgt gagataaaat
     4441 tcctttttat tacctctcca ataagttcga gatgagtttc gataggctta ctacccacag
     4501 aatcaagctc tacccagcat ccttcatctg ctaacttaaa gattacatcc ctatccttta
     4561 tgctatccgc atggactatt ataagtgcag aaggagaaac cttgtaactc tttataacat
     4621 ttagaacatc cagcgctgcc tccccttctc ccgtatgaca ggctatcgtt aaccctgttt
     4681 ctaaatgggt catcgctgaa gctgaaacca gtttacggtc tatttcactt agaggtccag
     4741 ggtcaacacc gatctttata aaacctggtc taataccagt atcctctatg ccatcacgat
     4801 actcctttat ccatctgttt gccaatttct cttctctctc ctcatatgca aactttggta
     4861 catatctatc atttgccgca ccatagtatc cggtattggt aataatatgc atatctaatc
     4921 tctccgacaa tttatgtaga agtcttatat cgcgtccaag ataggagggt gtacagtcca
     4981 caaacccagt gaaaccaaat ctctgaaggt cctccaggaa gggcgtcact ttttctatca
     5041 catcgtcagt atcatactta tcacgggatg taatatccat agcagaaaaa tcaaccagaa
     5101 catgctcatg taccagtgtc ctgccaagag aagaactatc tagcggtcct ctcacagtaa
     5161 ctatcataag aaattcctcc tgaacttttc tactattata ccaaaaagtt atgatataat
     5221 gaaaaaaatc tctaacagag gaggtaatta tgaaactctt aaactgtcaa atcgataatg
     5281 aaagatttat aggtctcctg gataataata gaacaataaa cctttccaag gcactaaaag
     5341 tctattctac catatacaga ggtgaagatt ggttcttcaa ttcaatagaa gatttactta
     5401 gcctgga
//
LOCUS       DAOTKU010000049         5359 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_13722_length_5359_cov_3.715498, whole genome shotgun sequence.
ACCESSION   DAOTKU010000049 DAOTKU010000000
VERSION     DAOTKU010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5359
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13722_length_5359_cov_3.715498"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      18..129
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            190..693
                     /gene="thiT"
                     /locus_tag="P5310_02040"
     CDS             190..693
                     /gene="thiT"
                     /locus_tag="P5310_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HRU32177.1"
                     /translation="MNRLNARLIAEIGLTLALGIVLHMIPLYQLPQGGRITPGSLVPI
                     FILSIRRGPKTGMLAGGVFGLLVYLIEPFFVHPIQFILDYPLAFSLLGLSGMVPNIPV
                     LGIILGMIGRLLSHIISGVVYFSSYAPQGINPWIYSITYNASVILPDAILALVITIII
                     LPRLPKR"
     gene            690..1337
                     /locus_tag="P5310_02045"
     CDS             690..1337
                     /locus_tag="P5310_02045"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HRU32178.1"
                     /translation="MKGILIANGELFDRDSLIREVKEDRFIICIDGGTNYAYQSDIIP
                     DLIIGDMDSVDNKVLEYYKTLDIPSRLYPRNKDKTDFHLAIEEIERAGIRDVSIYGIV
                     GTRIDHTLSAFGIIRRYVRERRLDLVKISIGVKADGYVFRDSIEIEGEISETISIIPL
                     TDKVSGVTTYNLKYPLKEAEIYWENSLTISNEIEKSPCRIEVSDGVILVAHYRSK"
     gene            complement(1311..2786)
                     /locus_tag="P5310_02050"
     CDS             complement(1311..2786)
                     /locus_tag="P5310_02050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HRU32179.1"
                     /translation="MILANSELMRLSESRVMEIFNVDEELLMEIVAVKVADRILKITG
                     NVKDKHFLVFAGRGKNGGDAIAIARHLILQGARISAFLIDDGGGSFSPLLRLQIDRLK
                     KIAPDSMRDFNFQEIRGDFVIDGLFGTGFKGEMREPYNSVVRYINNLNIPIISIDVPS
                     GLDASTGRVSSNCIHANYTFTLGLAKLGLFIYPGAGYGGEISLIDLGFPLDRYTDSSF
                     NLIDESVVKGVITRRNPDTHKGTYGRVGIVAGSYNYPGASVLVTKGALASGTGLVTLF
                     ISSNMFPMIHSIDPEVTFYPRENFKELFPVLSSIVVGPGLGKVEPSFLEDIITSSTVP
                     VLLDADGLNSIAGSLDILKSSNIPIVITPHPGEMSRLIGKSVSFVQDNRIDVASDFSR
                     EYGVITILKGARTVIASPSGEIYINPTGNPSMATGGMGDVLSGLIGGFIARGLSPVSA
                     SILGVYIHGLAGDVISREKERVLAQEVASEIPRLLGSIVSD"
     gene            complement(2798..3979)
                     /locus_tag="P5310_02055"
     CDS             complement(2798..3979)
                     /locus_tag="P5310_02055"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRU32180.1"
                     /translation="MDYDVVIVGGGPIGCFVGGNISERGYSVLILEEHPEIGEPTHCS
                     GLVSKDVISLSGVEEDVILNRIGGANIYAFDGTKLSFLGDDTYALVIDRVLFDRKIAE
                     KAEKKGAEILKGARAVGAEREDNKIIVKYTKDKEEFHIRSSILIGADGAYSSVARWFG
                     LPFPSEIIYGFQVEGELSCNNLAQVNVMIDNETSPGWFSWIIPVKENYARIGLGINVR
                     ENPRKYLDKLCKKWELLNTFSEDNIKKVMGGLIPLGLIGRSYTDNVILVGDAAAQLKP
                     LSGGGLYLGLLAGEICSDVVLDGLEKKDFSSSMLSKYHKLWMKSVGKEIRLGLRLRRI
                     FLGLSDEEKAELLEILDNQEAKSIILSAGHIDHPWKVAYKLMTHIKAPLMVRMLKLAF
                     S"
     gene            complement(3969..5114)
                     /locus_tag="P5310_02060"
     CDS             complement(3969..5114)
                     /locus_tag="P5310_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32181.1"
                     /translation="MTKREMNLRIFQGKDIPYPLFQPRIESWYNWHRQFNVMPERYKD
                     YSLLELYDELDISMRYIAYYTEIPSPVGLKYTDKVKINEKEVDSECRLITIDTPYGEL
                     TREIRLTSDRNWRTSRSCIKTREDIKKATWLFRNTEFYFIEENFDKGDKFIGDRGEPQ
                     FFLPRSPYQSLHVDSSWMTLEDLIYAITDLPSEIEELMNAIDESYDRLYEEIISCRDK
                     VRIINFGENIDAYLLSLKYFEEYLVPFWEKRSNQLRKAGIYTHIHIDGHFRPILKYLK
                     DLPFDGLEALTPEPQGDVLIEEMKEYIGDKVLLDGIPAILFLPIYSREELYSCVEKLI
                     RLFHPRLILGISDEIPEGVDEEGIERVRWISEYCKSFKTGKGDSYGL"
BASE COUNT         1620 a         1187 c          939 g         1613 t
ORIGIN      
        1 caaacataat atataatata accaggcagg ggagccttta tatttgaggc tgagaggaag
       61 gggttaaccc ttcgaccctc cgaacctgaa ctgggtaatg ccagcgtagg gagccatagc
      121 ggaaattcaa agggctgtag gctctcacgc ttacagccct ttatctttta ataaggaggt
      181 agatgcggta tgaacaggct caatgcaaga ttgatagcag agatagggtt gactttagct
      241 ctggggattg tactccatat gataccctta tatcagttac cacagggggg acgtattacc
      301 ccgggaagtc ttgtccctat atttatactg tcaatcagaa gaggaccaaa aacgggtatg
      361 cttgccggtg gagtctttgg tcttcttgta tatctgatag aaccattctt tgtccatccg
      421 atacagttta tattagatta tccactagca ttctctctgt tggggctttc aggaatggta
      481 cctaatatac ctgtactggg aataatcctg ggaatgattg ggagactttt aagtcatata
      541 atctcagggg ttgtatactt ttcatcatat gcaccacagg gtataaatcc ctggatctac
      601 tcgattacat ataatgcatc ggtaattctt cctgatgcga tcctggcatt agttataaca
      661 atcatcatac tcccaaggct tccaaagaga tgaaggggat actcatagct aatggtgaac
      721 tatttgatag ggactctctg ataagagagg taaaagagga caggttcatt atatgtatag
      781 acggtgggac taactacgct taccagtcag atatcatacc tgatttaata ataggggata
      841 tggactcagt cgataataag gtcttagagt actataaaac tctagatata ccatccagac
      901 tgtatccgag gaacaaggac aaaacagact ttcatctggc aatagaagag attgaaagag
      961 ctggtataag agatgtttca atatatggga tagttgggac aagaatagac cacacattat
     1021 ctgctttcgg tataataaga agatatgtaa gagagagacg gctagacttg gtaaagatat
     1081 ctataggagt aaaagcggat ggatatgtat ttagagacag tatagagata gagggagaga
     1141 tcagtgagac aatctccatt ataccattga ccgataaggt ctcaggtgtt actacttaca
     1201 accttaaata tcccctgaag gaggcagaga tttactggga aaatagcctt acgataagta
     1261 acgagataga gaaatctccc tgccgtatag aggtttcaga tggagttatc ctagtcgctc
     1321 actatagatc caagtaatct gggaatttca ctggctactt cttgagccag taccctctct
     1381 ttctctctac ttataacatc tccagctaac ccatgtatat atacccctaa gatgctggca
     1441 gatacaggag ataatcctct ggcaataaaa cctcctataa gtccactgag gacatccccc
     1501 ataccgccag tagccataga tggattacct gtcggattta tatatatctc accagatgga
     1561 gaagcgataa cagttctagc tccttttaag atcgtgataa ccccatattc tctactaaaa
     1621 tctgatgcta catctatcct gttatcctgt acaaaactta cactcttacc aataagtctg
     1681 gacatctctc cgggatgggg ggttatcaca attgggatat tagaagattt aagtatatca
     1741 agagaccctg ctatactgtt aagcccatct gcatccagta ataccgggac tgtactggag
     1801 gtaattatat cttctaggaa agaaggctct actttaccaa gtcctggtcc taccactata
     1861 gatgacagga cagggaaaag ctctttgaaa ttctctctcg gatagaatgt tacctccggg
     1921 tctatagagt gaatcatggg gaacatattg gaactgataa acagggttac aagccctgtc
     1981 ccgctggcaa gggcaccttt tgtaaccagc acagaggcac cagggtagtt gtaagacccg
     2041 gcaactattc caacccttcc ataagttcct ttatgagtat cggggtttct ccgggtaata
     2101 acacctttaa ccacagattc atctataaga ttaaaggatg aatctgtgta cctgtctaaa
     2161 ggaaatccca ggtctataag gcttatctca ccgccatacc ctgcaccagg gtatataaat
     2221 agccccaact tggctaaccc cagtgtaaat gtataattag catgaataca gttactggaa
     2281 actcttccag tacttgcatc cagtccagaa ggaacatcta tagatataat cggaatattt
     2341 aaattattaa tatatctgac gacagaattg taaggttctc tcatctcccc tttaaaacct
     2401 gtcccaaaga gtccatcaat aacaaaatca cctcttatct cctgaaaatt aaaatccctc
     2461 atggagtcag gagctatctt ctttaatcta tcaatttgaa gtcgtaaaag aggagaaaaa
     2521 gaccctcccc catcatctat cagaaaggca gatatccttg ccccctgaag aatgaggtgt
     2581 ctggctatag ctatagcatc accaccattt ttaccccttc cggcaaagac caaaaaatgc
     2641 ttatctttta cgtttcctgt aatctttaat attctatcgg caacctttac cgccactatt
     2701 tccatcaata attcttcatc gacattaaat atctccatta cccttgactc agacaatctc
     2761 ataagttctg aattcgccag tatcatctga accacctcta actaaaagcc aattttaaca
     2821 tacggaccat cagaggagct ttgatatgag tcataagttt atatgctacc ttccagggat
     2881 ggtctatatg tcctgctgag agtattatgg atttagcctc ttgattatca agtatctcca
     2941 gtaactcagc cttttcttca tctgagagtc ccaaaaatat tctccttaac cttagcccta
     3001 atcttatctc tttacccact gatttcatcc acagcttatg atatttggat aacatagagc
     3061 tggaaaaatc tttcttttcc agcccatcta aaaccacatc gctacatatc tcaccagcaa
     3121 gcagtccaag atataatcca cctccagaga gaggtttcaa ctgtgcagca gcatcaccga
     3181 caagtatcac attatctgta taacttctcc ctataagtcc tagaggtatc aaaccgccca
     3241 taaccttctt tatattatct tcgctgaaag tattgagtaa ttcccatttt ttgcacagtt
     3301 tatctagata ttttctaggg ttttctctta catttatccc gagtccaatc ctcgcatagt
     3361 tttccttgac aggaataatc caggaaaacc atcccggaga tgtctcgttg tcaatcataa
     3421 cattgacctg cgccaaatta ttacaggaca gctctccctc cacctgaaac ccatatataa
     3481 tttcagaagg gaaaggaaga ccaaaccatc ttgcaacact ggagtatgca ccatcggcac
     3541 ctataaggat ggaggacctt atatggaatt cttccttgtc cttagtgtac tttacaatta
     3601 ttttattatc ttctctttcg gcacctacag ctcttgcacc ttttaggatc tccgccccct
     3661 tcttttcagc cttctctgct atcttcctgt cgaaaaggac tctatctata acaagggcgt
     3721 aagtatcatc ccctaaaaaa gagagttttg tcccatcaaa ggcatatata ttggctcctc
     3781 ctatcctgtt aagaatcaca tcttcttcta caccacttaa agatataacg tctttagaaa
     3841 caagaccaga acagtgagtt ggctcaccta tctcaggatg ttcttccaga ataaggacac
     3901 tgtagcccct ctcagatata tttccaccca caaaacatcc gataggacct ccaccaacta
     3961 tcacgacatc ataatccata actatccccc ttccctgttt tgaaagattt gcaatattca
     4021 gatatccacc ttactctttc tatcccttct tcatccaccc cctcggggat ttcatcagag
     4081 ataccaagta ttaatctggg atggaagagc cttattaact tttccacgca actataaagc
     4141 tcttctctgg agtatatcgg caaaaaaaga atcgctggaa taccatcaag taggacctta
     4201 tccccaatat actccttcat ctcttctatg aggacatctc cctgtggttc aggcgtaaga
     4261 gcctctaatc catcaaaggg aaggtcttta agatatttta atattggtct gaaatgacca
     4321 tctatatgaa tatgagtata aatacccgct tttctaagtt gattagacct cttttcccaa
     4381 aaaggtacca gatattcttc aaaatacttc agggaaagga gatatgcatc tatattttct
     4441 ccaaagttga ttatcctcac cttatctctg caggatataa tctcttcgta tagacggtcg
     4501 taagattcat ctatggcatt cataagttct tctatctcag atggaagatc tgtaatggca
     4561 tagattaaat cttccagtgt catccaggaa ctatcaacat gaaggctctg atatggactt
     4621 ctaggaagaa agaattgggg ttcccctcta tctccgataa atttatcccc tttatcaaaa
     4681 ttctcctcta taaaataaaa ttctgtattt ctaaatagcc aggtcgcctt cttaatatcc
     4741 tctctggtct ttatacaaga tctagaagtc ctccagtttc tatcagatgt caatctgatc
     4801 tctcttgtaa gctctccata tggagtatct atagttataa gcctacattc actatccacc
     4861 tctttctcat ttatctttac cttatccgta tacttaagtc caacaggact cggtatttca
     4921 gtataataag ctatatatct catagagata tcgagctcat catacagttc caagagtgaa
     4981 taatctttgt atctctctgg catcacattg aactgcctgt gccagttata ccaagactct
     5041 attctgggtt gaaacagagg atatggtata tctttacctt gaaatattct cagattcatt
     5101 tctcttttag tcatctttcc ctccaaagca atatttgatt atcctattat aaaactttaa
     5161 agatgatttt gaaaaggtaa attatgataa aattataaac aggactgata tgaaaatatt
     5221 ggtagtgagt gaccacgaag aaccgatatt acatgagttt tttgatgagg atagatggaa
     5281 ggatatagat tttactatat cctgtggaga ccttccccct gaatttttat ctttccttgt
     5341 tacagtttta aaaggaccg
//
LOCUS       DAOTKU010000050         5347 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_13757_length_5347_cov_3.159108, whole genome shotgun sequence.
ACCESSION   DAOTKU010000050 DAOTKU010000000
VERSION     DAOTKU010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5347
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13757_length_5347_cov_3.159108"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..777
                     /locus_tag="P5310_02065"
     CDS             <1..777
                     /locus_tag="P5310_02065"
                     /inference="COORDINATES: protein motif:HMM:NF019736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HRU32182.1"
                     /translation="ENFYRSTERLKGEIFRYLSPLPDWLGFPNVEIGRIVPPSKRGEL
                     TVSVEDYNEFLIEKSAFVGPPLNIDGLEFIDRFDLFWKRKIYTLNMGHAILGYLGYLK
                     GYINVSQAIRDEWIRGVLIGAFNEVKELLLKLGLDNKDIDGYLETLLKRFVNENLGDT
                     VYRVGRDPIRKLGPEDRLVGPALECDKNGLHYDNLAIGIAGALLFDYPEDKMASELQS
                     KIKKDGLDNVLEEICKISPQSNLGIKIKEQYDSLRRLFGR"
     gene            774..1859
                     /gene="hypD"
                     /locus_tag="P5310_02070"
     CDS             774..1859
                     /gene="hypD"
                     /locus_tag="P5310_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009168486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HRU32183.1"
                     /translation="MSIPDYNKVQLARSILSEIVSISRRKITIMEFCGTHTHSIFRSG
                     IRDILPENITMLSGPGCPVCVTDNRDIDYCISLSSMPDTIIATFGDMIRVPGSLKSLQ
                     DVRAEGKDIRVVYSSMSALEIAENNQDKNVIFLGVGFETTAPTVAGSIIEAEKRGLDN
                     FFVYSMHKLTPPAMKAILDLGEVKLDGILGPGHVSTVIGSEAWNFLARDYAIPVSIAG
                     FDIIDILLGIKMLVEMIEKGEPKVINTYLRAVKPSGNRYALDIMNEVFDVTDSYWRGL
                     GMLPYSGLKIREKYRRFDASVQFPVEVKECVENKICRCGEVIRGVISPVECLLFDKIC
                     NPGNPVGPCMVSSEGTCSAYYLYGARR"
     gene            1856..2862
                     /gene="hypE"
                     /locus_tag="P5310_02075"
                     /pseudo
     CDS             1856..2862
                     /gene="hypE"
                     /locus_tag="P5310_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015407242.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
     gene            complement(2859..3347)
                     /locus_tag="P5310_02080"
     CDS             complement(2859..3347)
                     /locus_tag="P5310_02080"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HRU32184.1"
                     /translation="MIEVKIKAVGMDINSMSPVVILSDLEEKNFLPIWIGLPEAQSIA
                     MQIQQTPPPRPFTHDLMINILNELNISIKSVSIVDLKDGVYYATITLALDGKIHMIDS
                     RPSDAIALAVRTDAPIYVDEKVAAEAFILSESVQQQDNQEEEEFRQFLNNLKLDDLDK
                     YL"
     gene            3483..3788
                     /locus_tag="P5310_02085"
     CDS             3483..3788
                     /locus_tag="P5310_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32185.1"
                     /translation="MDREVIREMEEKRIAAMQTLDSVVRYIRDTKPSNLFAAGTGEEE
                     AGTKSKGSVTPPVSDAQMQALWQRWIGTVADVLEKANQVPGLMLDDVIKLFAKEEKK"
     gene            3799..4068
                     /locus_tag="P5310_02090"
     CDS             3799..4068
                     /locus_tag="P5310_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32186.1"
                     /translation="MPKNLDHIKAAKVLLGWSTYPLHSLIDSSSQVKGLGHGFTGHNI
                     DIIKAAETFLGEKGRLEATLHILQDIGLVEESDWKPIWESQRRKR"
     gene            4147..5097
                     /locus_tag="P5310_02095"
     CDS             4147..5097
                     /locus_tag="P5310_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013402452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HRU32187.1"
                     /translation="MPEVIGLGEIMVVMDPLRNGPLSSVENFRKAVGGAEGNTCVGVS
                     RLGCSSGWIGRLGNDEFGKFILSSLKGENVDTSCVKTVEGFTGVYFKERRGLGDTRAY
                     YYRKGSAGSTVSPEDIDSSYLKSAKFFHTTGITLALSETSRSAVNCGIDLAKRLGIKV
                     SFDVNLRLKLWSKEIAKEEIGKIIPKIDILFLTLDEASILFGMDKPEEIIKSLLSLGP
                     QIVGVKLGREGSMVGNFDEGIYHIPAFRDFCSVDNVGAGDGFVAGFLVGQLKGLGLKD
                     SAVLGSVAGAYATTTTGDIDGLPTWDEVEEFLGKRESIRR"
BASE COUNT         1671 a          776 c         1320 g         1580 t
ORIGIN      
        1 gaaaattttt atcgttctac agaaagatta aagggagaaa tctttcgata tctttctcct
       61 ctgccggact ggctcggatt tcccaatgtg gagattggta gaattgtacc ccccagtaag
      121 agaggggaac ttactgtaag tgtagaggat tacaatgagt ttctgataga gaaatctgcc
      181 tttgttggtc ctcctttaaa tatagatgga cttgagttta tagatagatt tgatctcttt
      241 tggaagagga agatatatac attaaatatg gggcatgcga tattgggata tcttggatat
      301 ctgaagggtt atataaatgt ctcacaggct atacgggatg aatggataag aggggtactg
      361 attggggctt ttaatgaggt taaagagttg ctattaaagt tgggtctgga taataaggat
      421 attgatggat acctggagac actcttaaaa agatttgtca atgaaaacct aggtgatact
      481 gtatatagag ttggaaggga cccgataaga aagcttggac cggaggatag acttgtcggt
      541 ccagctttag agtgtgataa aaatggattg cactatgata atcttgctat cggtatagca
      601 ggagcactgt tatttgatta tccagaggat aagatggcgt cagaattaca gagtaagatt
      661 aaaaaggacg gattagacaa tgttttagag gagatatgta agatctctcc tcaatctaat
      721 ctaggtatta agataaaaga acagtatgat tctttaagaa gactttttgg tagatgagta
      781 tccctgatta taataaagtt cagcttgcca ggagtattct ttctgagata gtttctattt
      841 ccagaagaaa aattactata atggaattct gtggaactca tacccattct attttcaggt
      901 ctggtatcag agatattctc cctgaaaata ttaccatgtt atcgggtcct ggatgtcctg
      961 tatgtgtaac cgataataga gatatagatt actgtatatc tttatcatca atgccggata
     1021 ccataatagc gacatttggt gatatgataa gagttcctgg tagtttaaag agccttcagg
     1081 atgtaagggc agagggtaaa gatataagag tggtgtacag tagtatgtct gccttagaga
     1141 ttgcagagaa taatcaggat aagaacgtta tatttttggg ggtaggattt gaaactaccg
     1201 ctcccaccgt tgctgggtct attattgagg ctgaaaagag aggtcttgat aattttttcg
     1261 tatattctat gcataaactt acacctccgg cgatgaaggc aatcctcgat ttgggagagg
     1321 taaaactgga tgggatactt ggtcccgggc atgtcagcac tgttataggt tcagaagcct
     1381 ggaactttct ggcaagagat tacgctatac ctgtatcaat agcaggtttt gatatcatag
     1441 atatacttct cgggataaaa atgctggtgg agatgattga aaaaggggag ccaaaagtta
     1501 taaatacgta tctgagggcg gtaaaaccgt cgggtaatcg atacgccctg gatataatga
     1561 atgaggtatt tgatgttaca gattcatact ggagagggtt gggaatgctt ccatatagtg
     1621 gattgaaaat cagagaaaaa tacagaagat ttgatgccag tgtacaattc cctgtagagg
     1681 tcaaagagtg cgttgaaaat aagatatgtc gatgtggaga ggttataaga ggagtgatat
     1741 caccggttga atgtcttctc tttgataaga tctgtaatcc aggtaatcca gttggaccct
     1801 gtatggtatc ttcagagggg acctgcagtg cctattatct atatggagca agaagatgaa
     1861 tgaagagaag ataacccttt cctatggaag tggtggaagg cttactcata aactgataaa
     1921 agatatattt atcagacatc ttgataatag tattctctca cagatgaatg attctgcgac
     1981 atttgagctg aatggtagaa tagcctttac tactgatagt tatgttgtga atccaatgtt
     2041 cttcccggga ggagatatag gaagattggc ggtaaatgga actgtaaatg atatagctat
     2101 gatgggagca gagccactct atataagtct tggtgtgata atagaagaag gattgttgtt
     2161 agaggaattg gagaaagtgg tcatatcgat aaaagatgca gcagaagagg ctggaataaa
     2221 ggtagtaact ggagatacta aggtggtaga aaagggtaag gcggataaat tatttataaa
     2281 tacctccggt gtaggagtta tacctgaggg tatagatata aggggagata acgctagacc
     2341 tggagatttg ataattgtaa gtggtaccat aggagaccat gggattgcta tactgagcga
     2401 gaggggagca cttggctttt cagccaatta gagagcgaca cctctcctct taatagattg
     2461 gtagcgagta tgatagatac ggttcctgat atacatgtgc tcagagaccc tacaagaggt
     2521 ggtttggcag agactctaaa tgaaatagcc caggcttcta atgctggaat agagatatac
     2581 gagtcagaga tccctgtaaa aggagcagtt aaaaatgcct gtgatttact gggacttgac
     2641 cctctttata tagcaaatga aggaaaactg atcgctatag ctcctgaaga gagagtaagt
     2701 ttacttctgg atataatgca cagttcccaa tatggaaagg atgcaaaagt tataggaaaa
     2761 gttaccggga gtcatcctgg gattgtagct ttaataacca aattgggggc aaagagaatc
     2821 ctcgatatgc cctcgggaga gcttctcccc agaatctgct agagatattt gtccaggtcg
     2881 tcaagcttta gattgtttaa gaactggcga aactcttctt cctcttgatt gtcctgctgt
     2941 tgaacacttt cactcaatat aaatgcttct gcggctactt tctcgtccac atatatgggg
     3001 gcatcggtcc tgaccgctaa tgctatggca tcacttggtc ttgagtcaat catatgaatc
     3061 tttccgtcaa gtgcaagtgt tatagttgca tagtataccc catcctttaa atccacgata
     3121 gatacactct ttatagatat atttaactca tttagaatat ttatcataag gtcgtgtgta
     3181 aatggtctag gtggaggtgt ttgttgaatc tgcatagcta tggactgtgc ctctggaagt
     3241 cctatccaga taggtaaaaa gtttttctct tcgaggtctg ataagataac tacaggagac
     3301 atcgagttta tatccattcc tactgcctta atcttcactt ctatcataat aatcacctct
     3361 cctttaatac ttcataatta taccacagtc tactagaaag ttaaactgct tttgtaattg
     3421 taacagtata attcctatgc tataatttag aaaaatttat gtttaagcag gaggattgag
     3481 aaatggatag agaagtaatc agagagatgg aagagaaaag aatagctgct atgcagactc
     3541 tggattcggt ggtcagatat ataagggata caaaaccctc taatctattt gcggcaggga
     3601 caggtgagga ggaggctgga actaaatcaa aggggtcagt tactcctcct gtaagtgatg
     3661 cacagatgca ggctctatgg cagagatgga tagggactgt ggcagatgtg ttggaaaaag
     3721 caaatcaggt tcctggactt atgttagacg atgttataaa attatttgct aaagaggaaa
     3781 agaaataaat agttagctat gcctaagaat ctggatcata taaaggcagc taaggttctg
     3841 ttaggatgga gcacatatcc tctccatagt cttatagatt caagttctca ggtaaagggt
     3901 ttagggcatg gttttactgg gcataatata gacattataa aggcggctga aacttttctt
     3961 ggggagaagg gaagattaga agccactctt cacatacttc aggacatcgg actggtggaa
     4021 gaaagcgatt ggaagcctat ttgggaaagc caaaggagaa agagataaaa taatcccact
     4081 tgacagaagg ttaattccat attatattaa aactaggttg tataaaggta aactttagga
     4141 ggtgtgatgc ctgaagtaat tggattagga gagataatgg tcgtaatgga cccactgaga
     4201 aatggtcctc tgagtagtgt ggagaacttc agaaaagcag ttggaggagc agagggtaat
     4261 acctgtgtgg gagtttcaag attgggatgt tcttctggct ggataggaag attaggaaat
     4321 gatgaatttg gaaaatttat actatcctcc cttaaaggag aaaatgtaga tacatcctgt
     4381 gtaaaaactg ttgaaggatt cactggcgtt tattttaaag agagaagagg cttaggtgat
     4441 accagggctt attattacag aaaaggctct gctggcagta cagtatctcc agaggatata
     4501 gattctagct atcttaagag cgccaaattt tttcatacta caggtataac cttagctcta
     4561 agtgaaactt caaggtctgc ggtaaattgt gggatagatc tggcgaaaag actgggtata
     4621 aaggtttcat tcgatgttaa tttgagactt aaactctggt cgaaagaaat tgctaaggaa
     4681 gagattggta aaattattcc taaaatagat attctttttc ttaccctgga tgaagcatca
     4741 atactatttg gtatggataa acctgaagaa attataaaga gtttgctatc ccttggtccg
     4801 cagatagtag gcgtaaaatt gggtagagag ggctctatgg tgggaaattt tgatgagggt
     4861 atatatcata tcccggcctt cagggatttc tgttcggtgg ataatgtggg agctggagat
     4921 ggttttgttg ctggttttct tgtaggacag ttgaaaggtt tgggtttgaa ggattctgcc
     4981 gtacttggca gtgttgcagg tgcctatgct acaactacaa caggagatat agatggatta
     5041 cctacctggg atgaggtgga ggaatttttg gggaaaagag agagtataag gaggtgagag
     5101 aggattagag aatgtgttat tagagttaat tttgagagac ttaaaacaca gggggcttca
     5161 ggttaattta aagaaggagg ctagttatta tgaaaagatt aaaatttttg gcaatactgg
     5221 ttttatcttt aagcttgatt ctgagtagtg tttcatcaag tttttctgcc gttattaagg
     5281 gtgaacagta taatctctca gattatgaga aactttctgg caaaaagatt ggcagattca
     5341 gtgagtc
//
LOCUS       DAOTKU010000051         5344 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_13768_length_5344_cov_3.663831, whole genome shotgun sequence.
ACCESSION   DAOTKU010000051 DAOTKU010000000
VERSION     DAOTKU010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5344
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13768_length_5344_cov_3.663831"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1640)
                     /locus_tag="P5310_02100"
     CDS             complement(<1..1640)
                     /locus_tag="P5310_02100"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011971159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 4 subunit A"
                     /protein_id="HRU32188.1"
                     /translation="MENRDNFVDVKIEQELQASYLDYAMSVIIGRALPDIRDGLKPVQ
                     RRILYAMLELGMEPNKPYKKSARVVGEVLGKYHPHGDAPVYEALVRMVQNFTLRYPLV
                     DGHGNFGSIDGDEPAAMRYTEVKLAPIAMELLKDIDKDTVDFMPNFDESLKEPVVLPA
                     GFPNVLVNGSSGIAVGMATNIPPHNLKEVIDALIYLIDHPEATVGDLMRYLPGPDFPS
                     GGIIRGKKGIIDTYTTGRGRITLEGKTEIEEHRGRKNLVIKELPYQVNKAVLIEKIAG
                     LIKEHKITGVQNIRDESDREGMRVVIELKKEAVPQVVINQLYKHTALRITYGSIMLVI
                     SDGVPKVMPLDEMLREFLEFRKDVVRRRTTFDLNKAKSRAHILEGLLIALDHIDEIIS
                     LIRSSKDVDAARRGLMENFDLSKEQAQAILDLRLQRLTALEREKVEEEYKEVTDTIRH
                     LEAILADEALLLGVIREELSLISRKFHQPRRTIIGEDIESETDEEDLLHNVDILITLT
                     ERGYIKRLPLDTYRKQRRGGKGIIGMETKDDRVSKTIAA"
     gene            complement(1652..3547)
                     /gene="gyrB"
                     /locus_tag="P5310_02105"
     CDS             complement(1652..3547)
                     /gene="gyrB"
                     /locus_tag="P5310_02105"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HRU32189.1"
                     /translation="MVDNHQYNASSITVLEGLEAVRRRPAMYIGSTGERGLHHLVYEV
                     VDNSIDEALAGCCSRIDVIINRDNSVTVIDNGRGIPVDIHPKEGRPALEVVMMTLHAG
                     GKFDSGTYRVSGGLHGVGVSVVCALSEWMEVEIKRDGYIWRQRYERGKKATELERGEE
                     TSETGTRISFKPDEKIFNNIEWNYDVISQRLRELAYLNKGLLISLKDERTEKEEEFIF
                     EGGIVAFVEGLNKNKRVLHNPIYISADSEKMSLEVCIQYNEGYTETVLSFANNINTIE
                     GGAHLVGFRSSLTRVLNEQARALKLLKESDQNLEGEDVREGLTAVISIKISNPEFEGQ
                     TKTKLGNPEVRPFVESVMSEKLSEYFAREKDTIRAILQKVVTAARAREAARKAREAAR
                     KVTSESITLPGKLADCQERDPRKSELYIVEGQSAGGSAKQGRDRRFQAILPLRGKILN
                     VEKSTLARSLSNEEILAIITALGTGVGDSFDLSKLRYHYVIIMTDADIDGAHLRTLLL
                     TFFYRYTRPLIENGHIYIAQPPLYLVKKGNIERYAYSDNELRNIITEIGEDGIMVQRY
                     KGLGEMNPEQLWQTTMNPETRVMLKVALEDAEKAETMFTLLMGIDVEPRRKFIEEHAT
                     FVRNLDI"
     gene            complement(3567..5192)
                     /locus_tag="P5310_02110"
     CDS             complement(3567..5192)
                     /locus_tag="P5310_02110"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRU32190.1"
                     /translation="MAEIKKETDLIPEEFWDELLPICNKYLGTRGEEVLNEALSVLGK
                     DRNNAVFGDLTDIFNALINKLVYLDRRADFRAELYALRRKYMVPPEAVKATPSKLELF
                     ISRFLTRRNAIALSIILVLLAGAGIYYVFKPDENSKISGKYDIVLWSQIYSRDMEETI
                     NEIIDNFNEKYKPYKSRFDLLPGSGDPMAEMGTQMKIMAVFAAGDPPDIIYGAPNPIF
                     INGPYLLKVERDYLQSLNYFPKILNQVAIDDKYYYCYPVSISPKMILIANKTLIEGLG
                     YNLGEIQERGISWDEFIDLADRLKLVTPYPVLVNLRGGTLFDVFWMSMVNNGGGMAVS
                     DGRFLWTDKKMEETIQFFDKLVKRDIINPNILGGRLSNNIEIFNNGKTGIIIGSYLDY
                     MMINNAGKVKAVILPFPNNKGNNLASLWDINGYFCFRQRDNYNTDKAQMTLMLAKTLT
                     ENSDWVSGTGALPASGLIWDRLNLYSEANHRFLYKYIDTSVPIDTSPLFNQIAFEAIN
                     PQLENIVTGKADVNQAMENIRNSLNRILSQNSK"
BASE COUNT         1373 a         1348 c          836 g         1787 t
ORIGIN      
        1 gctgctatgg tctttgatac cctgtcatcc tttgtctcca taccgattat tcccttacct
       61 cccctgcgct gtttcctgta tgtatcaaga ggcagtctct tgatataccc tctctctgtg
      121 agtgtaatga gtatatctac attgtggagt aaatcttcct catctgtctc actctctata
      181 tcctctccta ttatggtcct tcttggctga tggaattttc tggatatcaa tgatagctct
      241 tcccttataa cacccagaag cagggcttcg tcagccagta ttgcctccag atgtcttatt
      301 gtatctgtca cttctttata ttcttcttcc accttttctc tttccagtgc ggtgagacgc
      361 tgtaatctca gatccaaaat agcctgagcc tgttctttag agagatcaaa gttctccata
      421 agtccccttc ttgcagcatc aacgtcttta gaactcctta tgagagatat aatctcatct
      481 atatggtcaa gagctataag cagtccctca agtatatgtg ccctggattt agccttatta
      541 aggtcaaatg tggtccttct ccttaccaca tcctttctaa attccaaaaa ctctctaagc
      601 atttcatcca gaggcataac ttttggtact ccatcggata tgaccagcat aatactacca
      661 tatgtaatcc tgagagcggt atgcttatac agctgattta taacaacctg cggtacagcc
      721 tcctttttta actctatcac caccctcata ccttctctat ccgactcatc ccttatattc
      781 tgaacaccgg taatcttatg ttcttttata agtcctgcta ttttttctat aagaacagcc
      841 ttattcacct gatatggtaa ttcctttata accaaatttt ttcttcccct atgctcttct
      901 atctctgtct ttccctccag agttatacgt cctctacctg ttgtataggt atctattatc
      961 cccttctttc cccttataat cccgccggag gggaaatctg gaccagggag atatctcatt
     1021 aaatccccca ccgtcgcctc ggggtggtct ataagataga taagggcatc tataacttcc
     1081 ttaagattat ggggtggtat gttggttgcc atcccaacag ctatcccgga gctcccattt
     1141 accagtacgt tcggaaaacc tgccggaagt acaacaggtt cttttaaaga ttcatcaaag
     1201 ttgggcataa agtccacagt atccttatct atgtccttaa gaagctccat tgctataggc
     1261 gctaacttta cctctgtata cctcatagca gctggttcgt caccatctat tgagccaaaa
     1321 tttccgtgcc catcaacaag aggatacctt aatgtaaaat tctgaaccat tctaaccagt
     1381 gcttcataca cgggggcatc cccatgcggg tgatacttac ccagaacctc tccaaccact
     1441 ctggcagatt tcttatacgg cttatttggt tccattccca gttccagcat ggcatagaga
     1501 attcttctct ggacaggctt taatccatct cttatatcag gaagagccct gcctattatt
     1561 acgctcatag catagtccag atatgacgcc tgcaactcct gttctatttt tacatctaca
     1621 aaattatctc tgttttccat atctacctcc attagatgtc aagatttcta acaaaagtgg
     1681 catgctcttc tataaacttt cttctaggct caacgtctat ccccataagc agggtaaaca
     1741 tagtctcagc cttttctgca tcctccagag caactttaag cataaccctg gtctccggat
     1801 tcatagttgt ctgccagagt tgttctggat tcatctctcc caaaccctta tatctctgta
     1861 ccattatccc atcttctcca atctccgtga taatgttcct gagttcatta tcactgtacg
     1921 catatctctc tatatttccc tttttaacaa gataaagagg tggctgtgca atatatatat
     1981 gaccattctc tatgagtggt cttgtgtatc tatagaagaa tgttaacagt agagttctca
     2041 gatgagcacc atctatatct gcatcagtca ttattatcac ataatggtat cttaacttac
     2101 ttagatcgaa actgtctcca acacctgtcc ctagcgctgt tattatcgcc agtatctcct
     2161 cattggacag acttctagct aatgtactct tttccacgtt taatatcttt ccccttaagg
     2221 ggagtatcgc ctgaaatctt ctgtctctac cttgttttgc agaacctcct gccgactgtc
     2281 cctcaactat ataaagttca ctcttacggg ggtctctctc ctgacagtca gcaagttttc
     2341 ctggaagtgt aatactttca ctggtgactt ttcttgctgc ttctcgagct tttcttgccg
     2401 cctctcttgc ccttgccgca gttacaactt tctgtaagat ggctcgaata gtatcttttt
     2461 ctctggcaaa atattctgag agcttctcac tcatcacaga ttcgacgaat ggtctgacct
     2521 ccggattccc cagttttgtc tttgtctgtc cttcaaactc cgggttagat atctttatac
     2581 ttattacagc ggtaagacct tctctcacat cttctccctc aagattctga tcgctctctt
     2641 ttaatagttt gagagctctg gcctgctcgt tcagaaccct tgtaaggctg gatctgaaac
     2701 ctacaagatg tgctcctccc tctatagtat tgatattgtt ggcaaagctg agaacagtct
     2761 ctgtatagcc ctcattatat tgaatacata cctcaaggct catcttttct gaatctgccg
     2821 agatgtatat ggggttatgg aggacccttt tatttttatt aagtccctca acaaaggcta
     2881 caattccacc ctcaaagata aattcttctt ccttctctgt tctttcatct tttaatgaga
     2941 ttaatagccc cttattcagg taagccaatt ctctgagtct ctgggatatt acatcataat
     3001 tccactcaat attattaaat atcttctcat caggtttgaa gcttattctg gtaccggtct
     3061 ccgaggtttc ttctcccctc tccagctcgg ttgccttttt acctctctca tatctctgcc
     3121 tccagatata accgtccctc tttatctcca cttccatcca ttcagaaagt gcgcacacca
     3181 cagagacacc aacaccatgg agtcctccag agaccctgta agtcccacta tcaaacttcc
     3241 ctccagcatg gagggtcatc atcactactt ctaaggcagg tcttccctct tttggatgga
     3301 tatctacggg gatacccctg ccattatcaa taacagtcac actgttgtct ctatttataa
     3361 ttacatctat ccttgagcaa caacctgcca gggcttcatc tatgctatta tccacaacct
     3421 catatacaag gtgatgaagc cccctctctc ctgtacttcc tatatacata gcgggtcttc
     3481 ttcttaccgc ttcaagtcct tccagtacgg tgatagaact tgcattatat tgatgattat
     3541 cgaccaagct attcgcctcc tatgatttac ttgctattct gggataagat tctattcagg
     3601 ctatttctta tattctccat cgcctgattt acatcagcct tacctgtcac tatattttct
     3661 aactgcggat ttattgcctc aaaagctatc tggttgaaga gtggcgaagt atcaatggga
     3721 acggaggtat ctatatactt atacagaaac ctgtggtttg cttcactata gagattcaat
     3781 ctatcccata tcaatccaga agcaggcaag gctccggttc ccgataccca gtcagaattt
     3841 tctgtcaggg ttttagccag catcagcgtc atctgtgctt tatcagtgtt ataattatcc
     3901 ctctgtctga aacagaagta accgtttata tcccatagag aagccagatt attaccttta
     3961 ttatttggga atgggagaat caccgctttt actttaccag cattatttat catcatataa
     4021 tccagataac tcccaataat tataccagtc tttccattgt tgaatatctc tatattattt
     4081 gacagtcttc caccaagtat attcggattt ataatatctc tcttcaccag tttatcaaag
     4141 aactgaatag tttcctccat ctttttatct gtccacagga atcttccgtc acttacagcc
     4201 atcccaccac cattattcac catagacatc cagaacacat cgaaaagtgt gcctcctctc
     4261 aggtttacca gcaccggata tggggtgacc agtttcaatc tatccgccag gtctataaac
     4321 tcatcccagg agattcctct ctcctgaatc tcccccagat tatatccaag tccttctatt
     4381 aaggtcttat tggctatcag gatcatcttt ggagagatgg atacaggata acagtaataa
     4441 tatttatcat ctatagcaac ctggttcagt atcttcggaa agtaattcaa agattgtaaa
     4501 taatctctct ctaccttcaa cagatatggt ccatttataa atatggggtt tggggcaccg
     4561 tatataatat ctggagggtc tccagctgca aataccgcca taatcttcat ctgtgttccc
     4621 atctcagcca tagggtcacc actaccagga agtaaatcaa atcttgattt atagggttta
     4681 tatttttcat taaaattatc tataatctca tttatagtct cttccatatc tctggaataa
     4741 atctgagacc acaatactat gtcatacttt ccactaattt tagaattttc atccggctta
     4801 aagacataat atatcccagc tcctgccagc aggaccagta ttatactaag agcaatcgca
     4861 tttcttcttg taaggaatct gctgataaat aattctagct tacttggggt agcctttaca
     4921 gcctctggag gaaccatata cttcctcctt aaggcgtata actctgccct gaaatctgcc
     4981 cttctatcca gataaacaag cttatttatg agggcattaa atatatcagt caggtctcca
     5041 aagactgcat tgttcctatc tttccccagg accgacaggg cttcgtttaa tacctcctct
     5101 cccctggtcc ccagatattt gttacagata ggcaacagtt cgtcccaaaa ctcttctgga
     5161 atcagatcag tttctttttt tatctcagcc atagcagtaa taattatacc ataaattgcc
     5221 tgaaaaataa atactaacct agatttctgg ctattttata gaataactct tccaggatat
     5281 ttctcaatat tttttctgaa ccctcaatgg tctacgaaaa tgtcatctaa aatcttctaa
     5341 atat
//
LOCUS       DAOTKU010000052         5312 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_13850_length_5312_cov_3.122313, whole genome shotgun sequence.
ACCESSION   DAOTKU010000052 DAOTKU010000000
VERSION     DAOTKU010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5312
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13850_length_5312_cov_3.122313"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="P5310_02115"
     CDS             complement(<1..537)
                     /locus_tag="P5310_02115"
                     /inference="COORDINATES: protein motif:HMM:NF022727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3084 domain-containing protein"
                     /protein_id="HRU32191.1"
                     /translation="MNDLDLGLITFLIVISGVIAFAGNYVGRRAGKKRISILGIRPYY
                     TSMIITVITGVLITTITLIILINVSSSMRYALESKERLQETINLLSQKLSSTQSELSI
                     RADELSAAIEYQKKLEKNIQNQIEKVESLEKEVKKLDTALKEREKKLEEKELSLEENK
                     RKLNDLEAQREKLEKQIEE"
     gene            complement(530..1657)
                     /locus_tag="P5310_02120"
     CDS             complement(530..1657)
                     /locus_tag="P5310_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32192.1"
                     /translation="MSKLIMLTNSPGEIGGWFIPLARKIKTSYPEIDIISVLLPCQYA
                     SGTEFYVLQNSGLFSRTIDRKELLRFILSPSIDDDKKLILQLGGDPYWGTLLKIRLKS
                     KLYVYSDSMVHINRWADKILLTDPKFSRGADREVVIGNLILDSIDYSCYKEGPAVVFL
                     PGSRPYMLEKILPLMLETAEILGDSFNVPIKFIISPFIRLEDLDRYTPDNFKLVEDRF
                     ISPSGRTFYINKGGSWEWTEGVQLAINLPGTNTLQLALLGIPQITILPLQWADSIPLE
                     GIIEWIGRIPVLGRRIKHLVVYKALPKIKFIALPNIISGEKITEELIGEITPEVIAQR
                     IIFFLENRSLLEDMKKRLKGIKFLSGASEKIASIIGDELCE"
     gene            complement(1650..>2066)
                     /locus_tag="P5310_02125"
     CDS             complement(1650..>2066)
                     /locus_tag="P5310_02125"
                     /inference="COORDINATES: protein motif:HMM:NF014714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HRU32193.1"
                     /translation="RNNIYIPIVSSNKYDYMNASNLLIMASGTAVLEASLLEIPAIAL
                     YKLSWLSWLIAKRIVHHPYATMPNILLDKPVVIELLQKDANPGKLRDTLRQLLENPKR
                     IEEIKKELFEVKKILGEGKTAKKAADFILKGLDCIE"
     assembly_gap    2067..2076
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2077..2836)
                     /locus_tag="P5310_02130"
     CDS             complement(<2077..2836)
                     /locus_tag="P5310_02130"
                     /inference="COORDINATES: protein motif:HMM:NF014714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32194.1"
                     /translation="MGYTGLPRKHVKIAILAGEVSGDIQGSYLAGELLNNEFGIKVVL
                     CGIGGEYMRGRGVKLWAESTRYGSVGFLEPVRFLPGWLKLYNTVKDAIKKENPDAVVF
                     IDNQGFNLQIAKFIKKEKLNINMFYYFSPQAWLWGNSVAKQVKRLGIKVIAVFSKEAD
                     VYKETGAEVYFAGHPLLDIVEARVPPQKIRETLGIKDELPLIGLFPGSRYQEVSSLLP
                     VMLEGIAPLINREAYFYIASASSFTYEWITKILER"
     gene            complement(2803..3627)
                     /gene="lpxA"
                     /locus_tag="P5310_02135"
     CDS             complement(2803..3627)
                     /gene="lpxA"
                     /locus_tag="P5310_02135"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HRU32195.1"
                     /translation="MTYIHPTAIIDDSVSLGSDVEIGPYVVIKNDTVVGNGTKIDSHC
                     IIGPWVTIGNRCEIYGGASIGAPPQDIRYKGDRSYIIIGNNNIIREYVTIHRATGKDK
                     ATIIGDDNFLMAYSHIAHNCKIGNGVTMANSTNLAGYVEIFDKAVIGGIVGIHQFVRI
                     GRLAMVGACSKVVQDVLPFSIVDGHPAMAYGINTVGLRRAGYSPEMRSLIQKAFRIFY
                     FSNPNRTKSLTILEEELNDIPEIEDMISFVKNSKRGIVKASKEALQWDIQAFLENM"
     gene            complement(3593..4084)
                     /gene="fabZ"
                     /locus_tag="P5310_02140"
     CDS             complement(3593..4084)
                     /gene="fabZ"
                     /locus_tag="P5310_02140"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HRU32196.1"
                     /translation="MLDRKISELNICDLLDIMPHRYPFLLVDKIVEFEIGKRVKGIKN
                     VTINEPYFTGHFPELPIVPGVMILESMAQVAGTLLLKEAKESGEIAMLIGVDRVKFRK
                     VVKPGDQMNIQAELIQRKSSFCKFKSNVYVDNSLVAEGELLIVLYKEESIRDLYTSNS
                     DNR"
     gene            complement(4074..4889)
                     /gene="lpxC"
                     /locus_tag="P5310_02145"
     CDS             complement(4074..4889)
                     /gene="lpxC"
                     /locus_tag="P5310_02145"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:TIGR00325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HRU32197.1"
                     /translation="MFQTTINRSIEIKGIGLHTGKESRIIISPSIPNRGVIFNFKGKE
                     IIEAKVDLVTSTNRGTILGYNGYSLYTVEHILSALNGMEIDNVEIIVDGEEIPSCDGS
                     AKVFVEKIKEAGISNTSMEREIINLPEPIWIINGEKSITVFPDKKLSIYYMIEFPRIG
                     KQWIFYEHSPLNYEMDIAPARTFGFEEEIEMLLSAGLAKGGSYLNSIIVGENGYSTSL
                     RYPDELVRHKLLDLMGDLYLLGRRINATIVVVKGGHNLHVELVKKIKEVLDVR"
     gene            complement(4867..>5312)
                     /locus_tag="P5310_02150"
     CDS             complement(4867..>5312)
                     /locus_tag="P5310_02150"
                     /inference="COORDINATES: protein motif:HMM:NF025953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HRU32198.1"
                     /translation="IRIPHLGNVVIEDDVEIGANSVVDRSTLGSTIIKRGSKIDSLVL
                     VAHNVRIGEDTVIAGQTGIAGSCLIGNRCMFGGQVGVADHIKIGDNTVIFAQSGVAGD
                     VPPNSVISGTPARPHREWLRAQALLLRLPKILRRLGLKDVSDDDQ"
BASE COUNT         1493 a         1241 c          793 g         1775 t
ORIGIN      
        1 ctcttctatc tgtttttcca gcttctctct ctgtgcctcc aggtcattta attttctttt
       61 attttcttct aaagatagtt ccttctcttc cagcttcttc tctctttctt tcaatgcggt
      121 atctaatttt ttaacttctt tttctaaaga ttcaaccttt tcgatctgat tctgtatatt
      181 cttttccagc tttttctgat actctatagc tgctgaaagc tcatctgctc ttatagaaag
      241 ttcagattgt gtggagctta atttttgaga cagcaaattt atcgtctcct gtagtcgctc
      301 tttactctcc agggcatatc tcatactgga ggatacatta atcaaaatta ttaaggttat
      361 tgtggtgata agaactcctg ttatgactgt tataatcata gaagtgtaat atggtcttat
      421 acccaggata gagattcttt tcttccctgc ccttctacca acatagttac cggcaaaagc
      481 tataactcca cttataacta taagaaaggt gataagcccc aaatccagat cattcacaga
      541 gctcatctcc tataatggaa gcgatcttct cactcgctcc actcagaaat tttatacctt
      601 ttaatctttt tttcatatcc tccagtaatg atcggttttc cagaaagaat attatccttt
      661 gagctatgac ttcaggggta atttctccta tcagttcttc agtaattttt tccccggata
      721 ttatatttgg aagagcgata aacttgattt tgggtaaagc tttatacact accagatgtt
      781 ttattcgtct gcccaggaca ggtattctgc caatccactc tataatacct tcaaggggta
      841 tagaatctgc ccactgtaaa gggagtatag ttatctgagg tattcctaga agagctaatt
      901 gcaaggtatt cgtccccggt agatttatag ccagttgaac cccctctgtc cattcccaac
      961 tacccccttt atttatataa aaggttcttc cggatggaga gataaaccta tcttccacca
     1021 gtttgaaatt atctggagta tacctatcca ggtcctccaa tctaatgaac ggtgaaatga
     1081 taaatttgat tggaacatta aatgaatcac caagtatttc tgcggtctct aacatcagcg
     1141 gtaatatctt ctccagcata tatggtctac tacctggcag aaaaaccact gcaggaccct
     1201 ccttatagca ggagtaatct atactatcaa gtatcaaatt ccctataact acctctctat
     1261 cggcacctct agaaaattta ggatcggtaa gaagaatctt gtctgcccat ctatttatat
     1321 gtaccatact gtcagaatat acataaagct tggattttaa cctgatttta agcaatgttc
     1381 cccaataagg gtctccgccc aactgaagaa taagcttttt atcatcatct atggatggtg
     1441 ataaaataaa tcgcaacagt tctttcctat caatcgtcct agaaaatagc cctgaatttt
     1501 gcaatacata aaattctgtt ccactggcat actggcaggg taataaaacc gaaattatat
     1561 ctatctcagg ataagaggtc tttatctttc tagctaatgg gataaaccac ccacctatct
     1621 ccccggggct gttggtaagc atgataagct tactcaatac aatctagccc ctttaagata
     1681 aaatcagccg cctttttagc cgttttccct tcccctaaaa ttttctttac ttcaaacaat
     1741 tctttcttta tctcctctat ccttttagga ttttccaata gctgtctgag agtatctctc
     1801 aattttcctg gattcgcatc tttctgcaat aactctatta ctacaggttt atccagtaat
     1861 atattaggca tcgtagcata gggatgatgg actattctct tggctataag ccaggagagc
     1921 cacgataatt tatagagtgc aatagccggg atttcaagta aagaggcttc taaaacagct
     1981 gtccctgaag ccataattaa caaattagac gcattcatat agtcatattt attactggaa
     2041 actataggta tatagatatt atttctnnnn nnnnnnttct ctcaagaatc tttgtaatcc
     2101 attcatatgt aaaactggac gcactggcta tataaaaata tgcctccctg tttattaagg
     2161 gagctatccc ttctaacata acaggtaaaa gagagctaac ctcctggtat ctgctccccg
     2221 ggaacaatcc tattaaagga agttcatctt ttatccccag tgtttctctt atcttctgtg
     2281 gagggactct ggcttctact atatccagaa gggggtgccc ggcaaaatat acctcggcac
     2341 cggtctcttt atatacatct gcctctttag agaagactgc tataactttt atccccagcc
     2401 ttttaacctg tttagctaca ctattccccc acaaccaggc ttgcggagaa aaataataga
     2461 acatattgat atttagtttt tcttttttga taaatttagc tatctgaaga ttaaaaccct
     2521 gattatcaat aaagacaaca gcgtctggat tctctttctt tatggcgtct tttactgtat
     2581 tataaagttt aagccaacct ggcagaaacc taaccggctc taaaaatcca acagacccat
     2641 accttgtact ctctgcccat aacttaaccc ccctgcccct catatactca ccaccaatac
     2701 cacagagaac tactttaatt ccaaattcat tattgagaag ctctcctgcc agataagaac
     2761 cctgaatatc tccagatacc tctccagcca gaatagcaat ctttacatgt tttctaggaa
     2821 ggcctgtata tcccattgta aagcttcttt agatgccttt actatccctc ttttactgtt
     2881 tttgacaaag gatatcatat cttctatctc tggtatatcg ttgagttctt cctccagtat
     2941 agttaaagat tttgttctgt tcggattaga gaaataaaat attctgaatg ccttctgtat
     3001 aagacttctc atctctggag aatacccggc tctccttagc ccaaccgtat tgattccata
     3061 agccatagca ggatgaccat ccactataga gaaaggaagt acatcctgta caacctttga
     3121 acaggctcct accatagcca atctgccgat tcttacaaat tgatgaatac ctactatgcc
     3181 tcctatgacc gccttatcaa aaatttctac atatccggca agatttgtag aattagccat
     3241 agtaacccca ttaccaatct tacagttatg tgctatatga gagtaagcca taaggaaatt
     3301 atcatcgcct attatggtag ccttatcctt tcccgtcgct ctatggatag tcacatattc
     3361 tcttattata ttattattcc ctataattat ataacttcta tctcctttat atctgatatc
     3421 ctgtggtgga gcacctatag aagcccctcc atatatctca catctatttc ctatagtaac
     3481 ccagggacct ataatacagt gtgaatcaat cttggtccca ttgcctacta cagtatcatt
     3541 tttaattacg acatatggtc caatttcaac atcacttccc agacttactg aatcatctat
     3601 tatcgctgtt ggatgtatat aagtcacgga tagactcctc cttataaaga actataagca
     3661 attctccctc tgccaccaga gaattatcaa catatacatt actcttaaac ttacaaaaac
     3721 tactcttcct ttgaatcaac tctgcctgta tgttcatctg atcccctggt tttaccacct
     3781 tacgaaattt caccctgtca actccgataa gcatcgctat ctctcctgac tcttttgctt
     3841 ctttaagcag tagagtacca gctacctgtg ccatagattc caggatcata actcctggga
     3901 caatcggtag ttccgggaag tgaccggtaa agtatggttc atttatcgtt acattcttta
     3961 tcccttttac ccttttacca atttcaaact ctacaatttt atcaaccagg agaaacggat
     4021 aacgatgggg cattatgtcc aagaggtcac atatatttaa ttctgatatc tttctatcta
     4081 acatctaaca cctcttttat cttcttcacc agctcaacat gaagattatg tcctcctttt
     4141 actaccacta ttgtagcatt aattcttcta ccgagtaaat agaggtctcc cataaggtcg
     4201 agtaacttat gccttaccag ctcgtctgga tatctgagag aggtggagta gccgttttct
     4261 ccaactatta ttgagtttaa ataacttcca cctttagcta acccggcaga gagtaacatc
     4321 tctatctcct cttcaaagcc aaatgttctg gccggagcaa tatccatttc ataatttaaa
     4381 ggactatgct cataaaatat ccactgtttt cctatccttg gaaactcaat catatagtat
     4441 atggagagtt ttttatcggg aaatacggtt atactctttt caccatttat tatccagatt
     4501 ggttctggga gatttattat ttctctctcc atactggtat tacttattcc tgcctctttt
     4561 atcttttcta caaagacctt tgcactacca tcacaggagg gaatctcctc tccatccaca
     4621 atgatctcca cattgtcaat ctccattcca ttcagtgcgg agagtatatg ctctactgta
     4681 tataaagaat atccgttgta tcccagaata gtacctctat ttgtagatgt aaccagatct
     4741 accttagcct ctataatttc tttaccctta aaattaaata ttacacctct atttggtatg
     4801 gatggagata ttataattct gctttccttt ccagtatgta agcctatacc cttaatttct
     4861 atgcttctat tgatcgtcgt ctgaaacatc tttcaatccc agtctcctca atatcttggg
     4921 aagtctcagt aatagagcct gagctctaag ccactctcga tgtggccttg caggtgtacc
     4981 tgagattaca gaatttggcg ggacatcgcc tgcaacgcca gattgggcaa agataacggt
     5041 attatctcca atctttatat ggtctgctac accaacctgt ccaccaaaca tacacctgtt
     5101 acctatcagg caactaccag ctattccagt ctgtccagct attacagtat cctcacctat
     5161 cctcacgtta tgtgctacca agacaaggct atctattttg cttcctctct ttatgatggt
     5221 agaacctaaa gtagacctat caactacact gttagctcct atctctacat catcctctat
     5281 aactacattc cccaggtgtg gaattcgaat at
//
LOCUS       DAOTKU010000053         5270 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_13977_length_5270_cov_3.430872, whole genome shotgun sequence.
ACCESSION   DAOTKU010000053 DAOTKU010000000
VERSION     DAOTKU010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5270
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13977_length_5270_cov_3.430872"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="P5310_02155"
     CDS             <1..327
                     /locus_tag="P5310_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32199.1"
                     /translation="DILIPNIKNNKEYLKNFFREDKKWEQMIDALYNKRNLLIHQGKI
                     FDVDLDDLNLLKFVIDKLLKFVFDISKDFQKPEQFEKLITNLSLDDEQLQENINILSY
                     IKRKRY"
     gene            complement(444..1136)
                     /locus_tag="P5310_02160"
     CDS             complement(444..1136)
                     /locus_tag="P5310_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32200.1"
                     /translation="MKRDLRVKYLILFIILASVAFLSGCKIHFSTEVYVSDLLALKNG
                     EEDIIYIPSTLKLQVISEESYLESKDKITRILKKYFGELDRISYESNNFESFYVAQLE
                     VIAREGSGKGTPVEGGLFSFRVREDNKNIVLAVRFNNRLFKQLENEFFKEFSQTVDTK
                     ELHLSITMINDLRNDVKLVAQGVYIGGYPCPFGASYTLKRREKIDIVFSDVLRDYLVN
                     SEDVEFANIILE"
     gene            1231..1851
                     /locus_tag="P5310_02165"
     CDS             1231..1851
                     /locus_tag="P5310_02165"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRU32201.1"
                     /translation="MANEEKIITLFLVRHGESIYGEKKLCGWSDVPLSDRGREQTIGL
                     AERLKDINFVKIVTSNMIRTIETAEIINRYHNLEIEVEPGIRELNYGLWEGKSREEID
                     KEFTDDYRRWKIDNSLAPTGGESALEVYTRSSMAIERIVKNTSGGNILIVAHKCVNRL
                     FICGILGGDLRYYRRVGQDNCALNIIRYYGDSPVIERINDTGVIYG"
     gene            1844..2791
                     /locus_tag="P5310_02170"
     CDS             1844..2791
                     /locus_tag="P5310_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRU32202.1"
                     /translation="MDKIIGIDIGGTKIAIGRADTRGKLEDSVRFPTDVSRGYRSILE
                     EIIEKTSKLFERDSVKAIGIGCGGPLDSKKGRILSPPNLPGWDDVPLVDDIKGVFNVP
                     VYLENDANAAALGEFYFGAGKDVSNMVYMTLSTGIGGGIILNNKLIHGVRDSGGEVGH
                     QTILPDGPLCNCGNRGCLEALSSGTGIAKIFREKLASGRESIVTSWVKEPEEISAKII
                     ADAAKAGDSLSKEVWDSAIYYLGIGISNIVTIVSPEMVVLGGSLTKYGESLFVNVREI
                     VKERARLVPVDEIKIVPAQLGDNVGILGAVAVGLLGDER"
     gene            2866..3153
                     /gene="rpsT"
                     /locus_tag="P5310_02175"
     CDS             2866..3153
                     /gene="rpsT"
                     /locus_tag="P5310_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017552603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HRU32203.1"
                     /translation="MPNIRSAEKQLRASRKKAERNKAAISRIKTETKKFLMYLNAKDV
                     NSAKTQISLVSSLLDKAAKKNIIHSNTASRKKSRLMKKLSLVESQSSATQA"
     gene            complement(3325..4902)
                     /gene="serA"
                     /locus_tag="P5310_02180"
     CDS             complement(3325..4902)
                     /gene="serA"
                     /locus_tag="P5310_02180"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HRU32204.1"
                     /translation="MRVLIADPISKKGIELLSKYCQVDVKTGLSKESLLEIIPLYDAL
                     IVRSETKVDRDVIFAGSKLKIIGRAGVGVDNIDVNAATERGIIVVNAPEGNTISACEH
                     TFALMLSLARNIPSANYSLKRGEWKRKEFTGVELYGKTLGVIGLGRIGLAVSQRALSF
                     GMEVIGYDPYIVGERITEMGIKPVSLDELYSRSDFITVHTPLNADTKDFINKDAFLKM
                     KQGVRIINCARGGIINERDLIWAIEEGIVAGAGIDVFEKEPPPADSLILQNDKIIVTP
                     HLGASTVEAQERVAQIVVEDVLRLLNGEPVRNGVNIGSIRNPEIFPFIKLGELLGKFA
                     AQVGDTPVSSIDIIYSGNITKFDCSPVTASIIAGVLQQGLTVPVNIVNALLVARQRGI
                     SVSEKRLSETEGFENLLTVELNGKVKVSGTLLENKEARLIKYNNYYLNVDFSDNMLLT
                     EHIDVPGIIGKIGTILGIKNINIAFMQVGRSENDAVMILAIDSPVDDEIIEEIKKIEN
                     IKLVRRISLGLKKTEED"
     gene            complement(4883..>5270)
                     /locus_tag="P5310_02185"
     CDS             complement(4883..>5270)
                     /locus_tag="P5310_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HRU32205.1"
                     /translation="LSEGLENSWKRHRLLGKATREGVKALGLKLLAKEEFASNAVTGV
                     YPPDGIQADELRKRVRDKGVTLAGGQASLKGKIFRIGHIGYILDTDIIVTLTVIGETL
                     KEMGYNVDPTLGVKKALEVFNEGTNS"
BASE COUNT         1625 a         1069 c         1001 g         1575 t
ORIGIN      
        1 gatattctta ttccaaatat caagaataac aaagaatact taaaaaattt ctttagagaa
       61 gataaaaagt gggagcaaat gatagatgct ctttacaata agagaaattt acttattcat
      121 caagggaaaa tatttgatgt cgacttagat gatttaaatt tacttaaatt tgttattgac
      181 aagttactta aatttgtttt cgatataagt aaagatttcc aaaagccaga acaatttgag
      241 aaacttatta caaatttaag cttagatgat gagcaattac aagagaatat aaatattctt
      301 tcctatatta agagaaagag atattagaga taaaaaaaga gttaaccacc atggatgtac
      361 aaatattaga ggaatataca ggaggcgata taacagattc tgtctgaatt tcaatcacac
      421 ccctttttct ctccccccat cccctactct aaaattatat tcgcaaattc cacatcttca
      481 gagtttacaa ggtaatccct gagaacatcg gaaaatacga tatctatctt ttcacgcctt
      541 tttaatgtat aacttgctcc aaatggacag ggatatcctc caatgtaaac tccctgggct
      601 accagtttta catcattcct caggtcattt atcatagtga tgctgaggtg taattctttg
      661 gtgtctacgg tctgagaaaa ttctttaaag aattcattct ccagctgctt gaagagacga
      721 ttattaaatc taacggctaa aacgatattc ttattatcct ctctgaccct gaaagaaaat
      781 aatccaccct ctacaggagt tcctttaccg gaaccttctc ttgctataac ttcaagctgt
      841 gcaacataga agctttcaaa gttattgctt tcatatgaaa ttctatccag ttctccaaaa
      901 tatttcttca ggattctggt tatcttatct ttactctcca ggtaagattc ttcagatata
      961 acctgcagct tgagtgttga aggtatatag attatatcct cttcaccatt tttaagtgcc
     1021 agtaaatcac tcacatagac ttctgtagaa aaatgaatct tgcaaccgga taaaaaagca
     1081 actgaagcta gaattataaa caggattaga tatttaaccc ttaaatctct tttcatatca
     1141 aactcatctc ctcttctttt tattttatgt ctgaataata ccagcatata aggtcaaatt
     1201 gtgtccctct gtggtaaaat agaaattact atggcgaatg aggagaaaat tataacatta
     1261 tttctggtaa gacatggtga gagtatctat ggagagaaga aactctgtgg atggagtgat
     1321 gttcctctct cagatagggg tagggaacag acgatcggtc ttgctgagag actgaaagat
     1381 ataaattttg tcaaaattgt aaccagcaat atgataagaa cgatagagac ggctgaaatt
     1441 ataaacagat atcataatct ggagatagag gttgaaccgg ggataagaga actcaactat
     1501 ggtctctggg aagggaaaag cagagaggag atagataaag agtttacgga tgattacagg
     1561 agatggaaga tagataacag cctcgctcct acaggtggag aatcagcact ggaggtctat
     1621 accaggtcat caatggctat agagaggatt gtaaaaaata cttctggtgg taatatactt
     1681 atagtagccc ataaatgtgt aaacagactt tttatctgtg gcattctggg tggagatctc
     1741 aggtactata gaagggttgg acaggataac tgtgcattga atataatcag atactatgga
     1801 gattctccag tgatagaaag gataaatgat acaggggtga tatatggata agataatagg
     1861 tatagatatt ggagggacaa aaattgccat tggcagggca gataccaggg gaaaactgga
     1921 ggattcggtg agattcccaa ccgatgtgtc cagaggttac aggagtatac ttgaagagat
     1981 catcgagaag acatcaaaac tatttgagag agattctgta aaagccatag gtataggttg
     2041 cggtggacca ctggattcta aaaaggggag gatattatcg cctccaaatc ttcctggctg
     2101 ggatgatgta cctctggtgg atgatataaa gggtgtcttt aacgttcctg tgtatctgga
     2161 aaatgatgca aatgcagctg ccctgggaga gttttatttt ggtgcaggaa aagatgtaag
     2221 taatatggtc tatatgactc tgagtacagg cataggtgga ggtataattc taaataataa
     2281 gttgatccat ggagtcagag atagcggtgg cgaggttgga catcagacca tactgccaga
     2341 tggtcctctc tgtaactgtg gtaacagggg atgcctggag gctctctcta gtgggacagg
     2401 aatagcaaaa atcttcagag agaagcttgc ctctggcagg gagtccatag ttaccagctg
     2461 ggtaaaagag ccagaagaga tatctgcaaa gataatagca gatgcagcca aggctggaga
     2521 ttctctatca aaagaggtat gggattctgc catatactat ctggggatag gtatatcaaa
     2581 tatagtgaca atagtaagcc ctgagatggt tgtcctgggg ggaagtctta ccaagtatgg
     2641 agaaagtttg tttgtgaatg taagagagat agtaaaagaa cgtgcccggc ttgttccagt
     2701 ggatgagata aagatagttc cagctcaact gggagacaat gttggaatcc ttggagcggt
     2761 agctgtcgga ctcctcggtg atgagagata gccgaccctt gaaagttaca gaagaattat
     2821 gttatactct ctggagaaat ttaagtagaa aatggaggta tacaggtgcc aaatataagg
     2881 tctgcagaaa aacaacttag agcttctaga aagaaagcag agagaaataa ggctgctata
     2941 tccagaatta agacagagac taagaagttt ctgatgtatc tgaatgcaaa agatgtaaac
     3001 agtgcaaaga cacagataag tcttgtctcc agcttactgg acaaggcagc caagaaaaat
     3061 attatacatt cgaatactgc cagcaggaag aagtccagat tgatgaagaa actcagtctg
     3121 gtggagtctc agtcttcagc tactcaggct tagagagaga ataaaatcct ctaagccttc
     3181 tcttggtttt acttctcctg tctttatcct tctctcggct gagagaagga gttttatttt
     3241 ttactctgat gacatcatta gacatctatc agagctatct ttacagagat atcttgacaa
     3301 ccctaatttc tatatatcta actcttaatc ctcttcagtc ttcttcaatc ccagagaaat
     3361 tctccttacc agttttatat tctctatctt ctttatctct tcaatgattt catcatccac
     3421 agggctatct atggcaagaa tcataactgc atcattctca cttcttccca cctgcataaa
     3481 tgctatattt atatttttaa tccccaggat tgttccaatc ttaccaataa tccccgggac
     3541 atctatgtgc tcggtgagaa gcatattatc actgaagtct acattcagat aatagttatt
     3601 gtactttata agtcttgcct ctttattttc aagaagagta ccggagacct tcaccttacc
     3661 attcaactcc acagtgagaa gattctcgaa accctctgtc tctgagagtc tcttctctga
     3721 tactgaaatg cctctctgtc tggctacaag taatgcattc actatattta ccggcactgt
     3781 taatccctgc tgtaacacac cggctataat actggcagtc acaggactgc agtcaaattt
     3841 cgttatattc ccactatata tgatatctat actacttaca ggggtatctc caacctgagc
     3901 ggcaaatttt cccaaaagtt cccctaattt tataaacgga aagatctccg gatttcttat
     3961 tgaccctata ttcacaccgt tccttaccgg ttcaccattg aggagtcgta acacatcttc
     4021 taccactatc tgggcaaccc tttcctgagc ctctacagtt gatgccccaa ggtgtggggt
     4081 cactattatc ttatcatttt gcagtattaa agaatcggct ggtggcggtt ctttctcaaa
     4141 tacatctatc cctgctccgg caactatgcc ctcctctatc gcccagataa ggtctctctc
     4201 atttataata ccacctctgg cacagttgat aattcttacc ccctgcttca tcttgaggaa
     4261 ggcatcttta tttataaagt ctttcgtatc cgcattcaaa ggggtatgaa cagttatgaa
     4321 atcactccta gagtacagct catccaaaga tacaggctta atccccatct ccgtaattct
     4381 ttctcctact atataggggt cgtatccgat aacttccata ccaaagctca gggctctctg
     4441 acttaccgcc aatccaattc ttcctaaccc tatgacacca agagtctttc cataaagttc
     4501 aacaccggta aactcctttc tcttccactc ccccctcttt aatgaatagt tggctgaagg
     4561 tatattcctg gcaagtgaaa gcataagagc aaatgtatgc tcacaggcag atatggtatt
     4621 cccttcaggg gcatttacaa caatgatccc tctctccgtt gcagcattta catctatatt
     4681 atccacacct acccccgctc tacctattat cttcagttta ctccctgcaa agatgacgtc
     4741 tctatcgacc ttagtctcac tcctcactat caatgcatca tacagaggta ttatctctaa
     4801 aagactctcc ttactgaggc cagtcttgac atctacctgg caatactttg ataaaagttc
     4861 tatccctttt ttgcttatag gatcagctat tagtaccctc attaaaaacc tccaatgctt
     4921 tctttactcc caaagtaggg tcaacattat agcccatctc ttttaaagtc tcaccaataa
     4981 cagtcaatgt aactatgata tctgtatcca gaatatatcc aatatgtcct attctaaata
     5041 ttttcccctt cagagaagcc tgtccaccgg caagagtaac ccctttatcc cttactcttt
     5101 ttcttaactc atcagcctga ataccatcag gtggatatac tccggtaact gcgtttgaag
     5161 caaactcttc tttagcaagt aatttcagcc ctaaagcctt taccccttct ctggtggctt
     5221 taccaaggag gcgatgtctc ttccaggaat tctccaatcc ttctgagagt
//
LOCUS       DAOTKU010000054         5212 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_14139_length_5212_cov_2.648245, whole genome shotgun sequence.
ACCESSION   DAOTKU010000054 DAOTKU010000000
VERSION     DAOTKU010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5212
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14139_length_5212_cov_2.648245"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="P5310_02190"
     CDS             <1..197
                     /locus_tag="P5310_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="biotin synthase"
                     /protein_id="HRU32206.1"
                     /translation="GIGPKSANRIIHFRKKEKIRDLETLKGLGVVVKRAKDFVLLDGK
                     RVRDKNIQLALFRDEIIKAV"
     gene            complement(194..1252)
                     /gene="ychF"
                     /locus_tag="P5310_02195"
     CDS             complement(194..1252)
                     /gene="ychF"
                     /locus_tag="P5310_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005544868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HRU32207.1"
                     /translation="MSLSWGLVGLPNVGKSTLFNALTRLNVPTSIYPFCTIDPHKGIV
                     PVPDERLYELASIIKPKKVIPATVEFWDIAGLIMGSSKGEGLGNEFLSHIRGVDGIIH
                     MVRCFDIPNIPHLGEDDPVRDIELINIELALSDLSIVERKLDKVRSLRRVGRGDPEEE
                     AVLEEIKERLLRGESIRDIPVIDDLPLLSWKPVIYVANTDSLDNPKVLRLIDWGKRNN
                     GIVIPVLAKIEEELSEFSDDEKKVFLRDGYGVDEPTIYKLVRESYRLLGLISFFTIWS
                     DEVRAWTVLSGTRAPQAAGKIHSDFEKNFIKVEVVPVEKLVSAGSWSRARELGYIRIE
                     GKEYTVNDGDVLHFKAGV"
     gene            complement(1249..1434)
                     /locus_tag="P5310_02200"
     CDS             complement(1249..1434)
                     /locus_tag="P5310_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004058210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HRU32208.1"
                     /translation="MEIDDILQLRKKHPCGSDLWKVVSLGVDVRLECLGCGRVISIDR
                     EEIDRKVKRIIKKVAIK"
     gene            complement(1442..2221)
                     /gene="trpA"
                     /locus_tag="P5310_02205"
     CDS             complement(1442..2221)
                     /gene="trpA"
                     /locus_tag="P5310_02205"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HRU32209.1"
                     /translation="MNRINEAFSRARSEHRVCFIPFYEFGYPDISNFYEIIELLSGYG
                     DIIEVGIPFSDPVADGPIIQKASDVAIKNGANLDKAFNWLYEFRKKDSITPVVFMIYY
                     NLIHNRGVREFFSIARDLDLDGVIIPDLPIEESDNVYPYRNGVSVIWLASITTGEERL
                     KKIIEKSDSFIYCVSLKGVTGPRDDISEEGLNLLGRVRTLSSIPTALGFGLKNPEQIR
                     SLVNITDGVIIGSEIIRIVDESNTEELERYLEGLKSATIMV"
     gene            complement(2218..3393)
                     /gene="trpB"
                     /locus_tag="P5310_02210"
     CDS             complement(2218..3393)
                     /gene="trpB"
                     /locus_tag="P5310_02210"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010880421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HRU32210.1"
                     /translation="MYNFPDERGYFGPYGGRFVPETLMEPVLELEQAYNKIKNDETFW
                     VEFDNLLREYVGRPTPLYYAGRLSERLGFKVYLKREDLCHTGAHKINNCIGQALLARR
                     MGKKRIIAETGAGQHGVATATVASLFGLECTVYMGEEDISRQKMNVFRMKLLGAEVIP
                     VSIGSKTLKEAINAALRDWVTNVDNTHYLIGSVIGPHPYPVMVRDFQSVIGKETREQI
                     LEREGRLPDYIVACVGGGSNSMGIFYPFLEDDVRLIGVEGGGLGLETKRHAASISNGQ
                     VGVLHGSRMYLMQDEWGNIMNTYSVSAGLDYPGVGPEHSYLEDTGRVRYQYATDEIAL
                     KGFRELSLLEGIIPALESAHAVGYLFEGDFPEGSTVIVNLSGRGDKDLDIVMKEMEL"
     gene            complement(3386..4021)
                     /locus_tag="P5310_02215"
     CDS             complement(3386..4021)
                     /locus_tag="P5310_02215"
                     /inference="COORDINATES: protein motif:HMM:NF012900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HRU32211.1"
                     /translation="MRIKICGITRIEDALMAGNLGAWAVGFIFAPSSSRRLSPEKARE
                     ISECIERHCPDLLRIGVFLDQSEEFISGVAGLCNLDSIQFHGKESPEFVKRFSGILKI
                     KAFTVDGSLSFRDMISRINSYRDCIPLLDLPKDRDISQDILIDIATELRDSEINFIIA
                     GGIDSTNICSFLKLNPFAIDISRGVEISPGIKDEVKMKELFSLVKGDDADV"
     gene            complement(4011..4766)
                     /locus_tag="P5310_02220"
     CDS             complement(4011..4766)
                     /locus_tag="P5310_02220"
                     /inference="COORDINATES: protein motif:HMM:NF012444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol-phosphate synthase"
                     /protein_id="HRU32212.1"
                     /translation="MSILDSIVLDKREEIPSLEDYRRRYKIVSSNFKDIFSRGKIAFI
                     AEVKPASPVKGEFQGIDKLEGVIKDLTDAPISAISVLTDRHFNASIENINLVRRYSGL
                     PILRKDFIIDELQVYESSFYEASAILLIARILDRAKLKSLYDLSLSLGMEPVVEIYGL
                     EDLEKVLAVDPEVILINNRDLDTFEVDIRHTEKMMRYIPLGISIISASGISKREDIEY
                     LNSLGIDGVLIGEAIMKSDKPGEKIMEMMGLAD"
     gene            complement(4750..>5212)
                     /locus_tag="P5310_02225"
     CDS             complement(4750..>5212)
                     /locus_tag="P5310_02225"
                     /inference="COORDINATES: protein motif:HMM:NF012800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HRU32213.1"
                     /translation="TIFNLLGPLLNPARLKAQLIGVYNEEIMDKLAEVVRILDMHPVV
                     IVHGLNDHLDEVSISGPTRVAVIYNSSIEKFIFDPKDVGLDLFPPESIKGGDARENAS
                     IISSILDGRGTPAQRTIVALNAAFALWIAGKAGSIEEGVRLVGGDLGVNFR"
BASE COUNT         1465 a         1319 c          898 g         1530 t
ORIGIN      
        1 cggggattgg accaaaatca gcaaacagga ttatccattt tagaaagaaa gagaagataa
       61 gagacctgga gacactgaaa ggactagggg tagttgtaaa gagagcaaag gactttgtgc
      121 ttcttgatgg taaaagggta agagataaaa acatacagct tgcactgttc agggatgaaa
      181 tcatcaaggc ggtctagacg cccgccttga agtgaaggac atccccatcg ttcacggtat
      241 actcttttcc ctctattctt atataaccga gttctcttgc tctggaccag gatccagcag
      301 agacaagctt ctctacaggg acaacctcaa cctttataaa gttcttctca aaatcagagt
      361 ggattttacc agctgcctgc ggtgcccttg tgccagagag gacggtccat gctctcacct
      421 cgtcagacca tattgtaaaa aagcttataa gtccaagtaa tctgtaagat tctcttacca
      481 gcttatatat ggttggttca tccaccccat acccatccct gaggaatacc ttcttctcat
      541 catcagaaaa ctcagagagt tcttcctcta ttttggctaa gaccgggata actattccgt
      601 tatttctttt tccccaatca atcaatctca ggacctttgg attatccaga ctatccgtat
      661 tggcaacata tatgacgggc ttccacgata ggaggggtaa atcatcgatg acagggatat
      721 ctcttatcga ttctcccctc agtagtcttt cctttatctc ttccagcaca gcctcttctt
      781 ccgggtcacc cctacccacc ctcctcaaag acctgacctt atcaagtttt ctctcgacta
      841 tcgagaggtc tgagagggct aattcaatat ttataagctc tatatccctt acagggtcat
      901 cctctccaag atgggggata tttgggatgt caaagcaacg gaccatatgt ataatgccgt
      961 ccacccccct tatatgagag agaaattcgt tgccaagacc ttccccttta ctggacccca
     1021 ttataagtcc tgctatatcc cagaactcta cagtagccgg tataaccttc ttgggtttaa
     1081 ttatactagc tagctcatat aatctctcat cagggacagg aacaataccc ttatgcgggt
     1141 ctatggtaca gaatggatat atagatgtcg gtacattcaa tctggtcagg gcattgaaga
     1201 gtgttgactt accaacgtta ggtagaccaa ccagacccca ggatagactc atttaatagc
     1261 cacctttttt attattctct tcaccttcct gtcaatctct tctctatcta tggatattac
     1321 cctgccacaa cccagacatt ccaatctgac atccacaccc agcgatacga ccttccagag
     1381 gtcacttcca caggggtgtt tcttcctgag ctgcaggata tcatctatct ccacagagat
     1441 attataccat aatagtggca gactttaatc cctcaaggta tctctccagc tcttctgtgt
     1501 ttgattcatc cactattctg ataatctcac tgccgataat aactccatcg gttatattta
     1561 ccagacttct tatctgttcg gggttcttca gaccaaaacc cagggctgtt gggatagaag
     1621 aaagagtcct tacccttccg agtaaattta atccttcttc cgatatatca tccctgggac
     1681 cggttactcc cttgagagat acgcaataaa taaaactatc actcttctct atgatctttt
     1741 ttaatctctc ttcacctgtt gtgatagaag cgagccagat tacggataca ccatttctat
     1801 atggatatac attatcagat tcttctattg gaaggtctgg tattataaca ccatccaggt
     1861 ccagatctct tgctatactg aaaaactccc ttacccccct attatggatg aggttgtagt
     1921 atatcataaa gaccaccgga gtgatgctgt ccttcttcct gaactcatag agccagttga
     1981 aagccttatc aaggtttgcc ccatttttaa tagcgacatc acttgccttc tgtattatcg
     2041 gtccatctgc tacagggtca gagaaaggga tacccacctc tataatatct ccataccctg
     2101 atagcagttc tattatctca taaaaattcg aaatatcagg ataaccaaac tcatagaaag
     2161 ggataaaaca taccctgtgt tcagaccttg ccctgctaaa tgcctcattt atcctgttca
     2221 tagttccatc tccttcatca caatatccag gtctttatct ccacgtccgg agagattcac
     2281 tatcacagtc gaaccttcag gaaagtcacc ttcaaataga tatccaaccg catgagcact
     2341 ttccagtgca gggataatac cttcaagtaa tgatagttct ctgaaaccct taagggctat
     2401 ctcatcggta gcatactggt atcttacacg acctgtatct tctagataac tgtgttctgg
     2461 accgacacca gggtaatcaa gtccagctga gacagagtag gtattcataa tgtttcccca
     2521 ctcgtcctgc ataagataca tcctggagcc atgcaaaaca ccaacctgac cgttagagat
     2581 actagcagca tgtctctttg tttctaaccc cagacctcca ccttcaacac ctatcaatct
     2641 cacatcatct tcgagaaagg gataaaagat tcccatagag ttgctccctc ccccaacaca
     2701 ggcaactata taatcaggta atctcccctc tctctccagg atctgctctc ttgtttcctt
     2761 tcctataacc gattgaaaat ctctaaccat cacaggatag ggatgaggtc ctattacaga
     2821 acctatcaga taatgtgtat tatcaacatt ggtaacccaa tctctcaggg cagcattgat
     2881 agcctccttt aaggtcttgc taccaattga tacagggata acctctgccc caagtagctt
     2941 catcctaaag acattcatct tctgtctgga tatatcctct tctcccatat atacagtgca
     3001 ttctaatcca aaaagactgg ctacggtagc tgtggcaaca ccatgttgcc cggcacctgt
     3061 ctcagctatg atacgctttt tacccatcct tctggcaaga agagcctgcc ctatacaatt
     3121 gtttatctta tgagcacctg tatggcagag gtcttctctc tttaaataaa ccttaaaccc
     3181 gagtctttct gaaagtctac cagcataata gagcggggtt ggtctaccaa cgtactctct
     3241 taagaggtta tcaaactcta cccagaaggt ttcatcattc tttatcttat tatatgcctg
     3301 ttctaactcc agaacaggct ccataagagt ctctggaacg aatctaccgc catatggtcc
     3361 aaaatatcct ctttcatcag gaaagttata catcagcatc atctcctttt accagactga
     3421 acaattcttt catttttacc tcatccttta tccctggaga tatctctact ccccttgaga
     3481 tatcaatagc aaatgggttc agttttaaaa aactgcatat attggtggaa tctatacccc
     3541 cggcaatgat aaagttgatt tctgaatctc tcagttcagt ggcgatatca ataagaatat
     3601 cctgagagat atctctgtcc tttggcaggt cgagaagcgg gatgcaatcc ctgtaagaat
     3661 tgattctgga tatcatatct ctgaaagaga gacttccatc cactgtgaaa gcctttattt
     3721 ttaatatacc gctaaatctt ttaacaaatt caggggattc tttaccatgg aactgaatac
     3781 tatcgagatt gcagagacca gctaccccag agataaactc ctcagactgg tccagaaaga
     3841 ctcctattct gagtagatcc ggacaatgtc tttctataca ttcagatatc tctctcgctt
     3901 tttcaggtga taatcgtctt gagctggagg gagcaaagat aaatcccaca gcccaggctc
     3961 caagattacc agccatcagg gcatcctcaa tccttgttat accacatatc ttaatccgca
     4021 agacccatca tctccattat cttttcccct ggcttatcag acttcattat agcctcacca
     4081 ataagaacac catctatgcc caggctattc agatactcta tatcctctcg tttcgatatg
     4141 ccgcttgcac ttattattga gatacccagt ggaatatacc tcatcatctt ttctgtatgc
     4201 cttatatcga cttcgaatgt atccaggtcc ctattattta taaggattac ctctggatca
     4261 accgccagaa ccttttctaa atcttccaat ccatatatct caaccaccgg ttccatacca
     4321 agagagaggg atagatcata tagagacttc aactttgcac gatccagtat cctggctatc
     4381 aacagtatgg ctgatgcctc gtagaaactt gattcataga cctgaagctc atctataata
     4441 aaatcttttc tcaggatggg aagacctgaa tacctcctca ccagatttat attttcaata
     4501 ctggcattga agtgcctgtc ggtcaataca gagatagctg atataggggc atcggtcaaa
     4561 tcttttatca caccttccag cttatctata ccctgaaact caccctttac aggagaggca
     4621 ggtttaacct ctgctatgaa agctatcttt cccctggaga agatatcctt gaaattagag
     4681 ctaacaatct tatatcttct cctgtaatct tcaagagaag gtatctcttc tctcttatcc
     4741 agaacaatgc tatctaaaat tgacacccag atcaccccct accagtctta caccttcttc
     4801 tatagaccct gcctttccag caatccagag ggcaaaagca gcattcaatg cgacgatagt
     4861 tctctgtgca ggggttcctc tcccatccag gatggaactt attatactgg cattttctct
     4921 tgcatctcca ccctttatac tctcaggggg gaataaatcc agacctacat ctttcgggtc
     4981 aaagataaac ttttcgatag aagaattata aataactgcc accctggtag gtcctgaaat
     5041 ggatacctca tccagatggt cgttgagtcc atgcactata accacaggat gcatatcgag
     5101 aatcctcacc acttcggcaa gtttatccat aatctcctca ttgtaaaccc ctatgagttg
     5161 agctttaagt cgggcaggat taagcagtgg acccaggagg ttaaaaatag tc
//
LOCUS       DAOTKU010000055         5194 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_14198_length_5194_cov_3.743724, whole genome shotgun sequence.
ACCESSION   DAOTKU010000055 DAOTKU010000000
VERSION     DAOTKU010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5194
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14198_length_5194_cov_3.743724"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1183)
                     /locus_tag="P5310_02230"
     CDS             complement(<1..1183)
                     /locus_tag="P5310_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HRU32214.1"
                     /translation="MSRAKSIMIQGTASSVGKSMLVTALCRIFTLDGFRVAPFKSQNM
                     ALNSFVTVDGGEIGRAQAVQAEACGIEPSVLMNPVLLKPSSDVGSQVIIKGKVFKNMT
                     AEEYQRFKPELREIIKDAYEELAANHDIVVIEGAGSPAEINLKDNDIVNMGMAEIADC
                     PVLIVGDIDKGGVFASIVGTMILLDQRERKRVSGIIINKFRGDVEILRPGLSMLEKII
                     KRPVLGVIPYMRIHIDEEDSATEHLRLTKEDGDLDVAIVYLPHISNFTDFEPLYSVNE
                     LKIRYVYSADTFGSPDLVIIPGTKNTIGDLLYLKYNKISEEIIRYAREGRLVIGICGG
                     FQMLGKEILDPYGIESDIKETEGLGLLDISTTIEGEKITAQTIIQLEPAISSIFPGLS
                     GS"
     gene            1331..1876
                     /locus_tag="P5310_02235"
     CDS             1331..1876
                     /locus_tag="P5310_02235"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HRU32215.1"
                     /translation="MKKVISPQTILYPAPSVLVSCVSGSGKANIITLAWVGTVCSEPP
                     MVSISVRPGRFSYKLISETKEFVINIPTTDIVDKVEYCGIHSGKDVDKFKETGLTPMP
                     ATKVMVPLIKECPVNIECKVKDEVHLGSHNLFLGEIVAIDIDEEILTEEGRINYLKAK
                     PIIYNNGEYWKLGERLPISHR"
     gene            1882..3264
                     /locus_tag="P5310_02240"
     CDS             1882..3264
                     /locus_tag="P5310_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HRU32216.1"
                     /translation="MELLTLEDVLKLNRKEIRNLYGEYVNSGLAKLLSLLDFDKSFVK
                     AQGQYVWDDEGNKYIDFLGGYGALNLGHNHPDVIKAIESINDSPNILQASLNRFAAGL
                     AHNLAAIAPAGLKHAFFSNSGAEAVESALKLARISTGKKTFVYCSNSFHGKSFGALSV
                     TGREKYRKPFEPLLQDIREVPFGDVSALEVALKPGDVAGFIVEPIQGEGGIIVPPDGY
                     LKSARELCSRYGALLIIDEIQTGFGRTGYIFACQKEDVSPDIMCLAKTLGGGIMPAGA
                     ILTTEDVWEKGYGGTEKCLLHTSTFGGNSWAMAAGIATIDIIVKENLPEQAKTKGEYF
                     LNKLISLKEKHKLIKDVRGRGLMLGLEFNQPEGGILDKITAGSVSKISSEYLGSLVAG
                     ELINRYHIITAYTLNNPNVIRLEPPLIVTKEDLDVLVNALDDLFTSHPTFTRIALAST
                     KTIFGSIFKR"
     gene            3275..4150
                     /gene="era"
                     /locus_tag="P5310_02245"
     CDS             3275..4150
                     /gene="era"
                     /locus_tag="P5310_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015758510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HRU32217.1"
                     /translation="MKSGLVTIIGRPNVGKSTLLNALIGEKVSIVSDRPQTTRKNILG
                     IVSKDDFQVMFLDTPGIHKPKDDLGEHMVKDAISALEDDVDLIIFIVDAKEIADEDIY
                     IDKYVLPKYQNKKILAINKSDLVNTDPIESVRNKINISNYDEVIPISALYGTNLDKLE
                     EAIKKYLPEGPQYYPEDVKTTEPIEDRIREIIREKVILLTYQEIPYSIAVVLEEFEER
                     NDLFYIRAVIFVEKESQKGIIIGKNGKKLKEIGTLAREELEALLGKKVYLDLWVKVRD
                     KWRKKARWVEELVYS"
     gene            4175..4897
                     /gene="recO"
                     /locus_tag="P5310_02250"
     CDS             4175..4897
                     /gene="recO"
                     /locus_tag="P5310_02250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HRU32218.1"
                     /translation="MNSYYITGIILSHENIGEKDRLITFLTEERGKLRAVARSSRRIS
                     SKMAGSLEPFNLVRCWMHTGKTFDIIKNVQLIKTFPNVIKNIDIYITASRMFEFIQVT
                     VQENDESQDMFRLLLGVLTALDRYDSLNSIKVFFWLNVLILSGYRMRLDRCVCGREDV
                     KGFSPSMGGVVCIDCMLKTNDSILISNSALDTLRMYEQIRLSSVVKREIPDTLMREID
                     TIIDRFRLCHTNIKLRSEEVTI"
     gene            4894..>5194
                     /locus_tag="P5310_02255"
     CDS             4894..>5194
                     /locus_tag="P5310_02255"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HRU32219.1"
                     /translation="MTFQEVILKLLNFWQEHNCIITQPYDMEVGAGTMSPDTFFRALG
                     REKWNVAYVQPSRRPADGRFGENPNRLGKYYQLQVILKPAPNNVQDIYIDSLRSLG"
BASE COUNT         1614 a          945 c         1133 g         1502 t
ORIGIN      
        1 tagaaccgga taacccggga aagattgaag aaattgcagg ttcaagctgt atgattgtct
       61 gagcagtaat tttttctccc tctattgtgg tggatatatc taacaatcca agcccttcgg
      121 tctctttgat atcagattct ataccgtaag ggtccagaat ctcctttccc agcatctgaa
      181 acccaccaca tatcccgatg accagtcttc cctctctggc atatcttata atctcctccg
      241 agatcttatt atatttcaga tataacaggt ctcctatagt attctttgtc ccggggatta
      301 taaccaggtc aggggagcca aaggtatctg cactgtatac atatcttatc tttaattcat
      361 tcacagagta caggggttca aaatcggtaa agttggatat atgtggcagg tatacgatgg
      421 cgacatccag atctccatcc tctttggtaa gtctgagatg ttcggttgcg ctatcttctt
      481 catctatatg aattctcata tacggaatga cacccagtac aggtcttttt attatttttt
      541 ccagcataga taaaccaggt cttaatatct ccacatctcc cctgaactta tttattataa
      601 ttccactgac cctctttctc tccctttgat ctagtagtat cattgtccca actatggagg
      661 caaaaactcc tcccttatct atatctccca caatgaggac aggacaatcg gctatctctg
      721 ccatccccat attaactata tcattatctt taagatttat ctcagcagga ctccccgctc
      781 cttctataac aactatatcg tgattagctg caagctcctc ataagcatcc tttataatct
      841 ctctgagttc tggtttgaac ctttgatatt cttctgctgt catatttttg aagaccttac
      901 cctttataat aacctgactt ccaacatcac ttgaaggttt aagcagtact ggattcataa
      961 gaactgatgg ttctatgcca caggcttcag cctgtaccgc ctgtgccctt ccaatctctc
     1021 ctccatcaac tgttacaaaa gaatttagag ccatattctg agacttgaaa ggggcaacgc
     1081 gaaaaccgtc cagggtaaat atcctgcata gagcagttac cagcatactc tttcctacag
     1141 aagatgctgt tccctgtatc ataatactct tagctctact catctatcca tcaccagatt
     1201 taagtataca ataaatatcc atcccagtct aatcagggtg agataatgca aattccacgt
     1261 tctcttgtct tatttttatc ctggtgatat tatttttata tataaaaaat tatagtttca
     1321 ggaggtgaag atgaagaaag ttatatctcc acagactatt ttatatcctg ctccaagtgt
     1381 acttgtgagt tgtgtatcag gtagtggtaa ggcaaatatt attaccctcg cctgggttgg
     1441 gacggtatgc tctgaaccgc ccatggtttc aatttcagta agacctggaa gattctccta
     1501 taagcttata tccgagacaa aggaatttgt tataaacata ccaactacag atatagtaga
     1561 taaggttgag tactgcggta tacactctgg aaaagatgtg gacaaattta aagaaacagg
     1621 gttaaccccg atgcctgcta ctaaggtaat ggtccctctc ataaaggagt gtcctgtaaa
     1681 tatagagtgt aaagtaaaag acgaggtcca tttaggttcc cacaacttat ttcttgggga
     1741 gatagtggct atagatatag atgaggagat tttgacagaa gagggaagga taaattatct
     1801 taaggctaag cccataatat ataataacgg agagtactgg aagttagggg agagacttcc
     1861 aataagtcac aggtgattca tatggaacta ctcacattag aggatgtatt aaaacttaac
     1921 aggaaagaga taagaaacct gtacggagaa tacgtgaact cggggcttgc aaagctccta
     1981 tctcttttag attttgataa gagttttgta aaggctcagg ggcagtacgt atgggatgat
     2041 gagggtaata aatatataga cttcttaggt ggttatggtg ccctcaatct tgggcataat
     2101 catccggatg ttataaaggc tatagagagt ataaatgata gtcccaatat acttcaggcc
     2161 tcacttaaca gatttgcagc tggtttagcc cacaatctgg ctgctatagc tcctgctgga
     2221 ctgaagcatg catttttctc caacagcggt gcagaggcgg tggaatctgc cttaaagctt
     2281 gccagaatat ccactggaaa gaaaacattt gtgtactgct ctaattcttt ccacgggaaa
     2341 tcttttggtg cactttctgt tacaggaaga gaaaagtatc gcaagccatt tgaaccatta
     2401 ttacaggata taagagaggt cccatttgga gatgtctcag cccttgaagt ggctcttaaa
     2461 cctggagatg ttgccgggtt tatagttgag cccatccagg gtgagggtgg aatcattgtc
     2521 ccccctgatg gatatttaaa gtcagcaaga gaactctgct ccaggtatgg ggcacttctt
     2581 ataattgatg agatacagac aggttttggc aggacaggat atatatttgc ctgtcaaaaa
     2641 gaagatgtct cacctgatat tatgtgtctg gcgaagactc taggtggagg gataatgcct
     2701 gcaggggcaa tacttaccac tgaagatgta tgggagaaag gttatggtgg gacagagaaa
     2761 tgtctcctcc atacatccac cttcggtgga aattcctggg ctatggcagc cggtatagcc
     2821 actatagata taattgttaa agaaaatctc cctgaacagg caaagacgaa gggtgaatat
     2881 ttcctgaata agcttatctc tcttaaagag aagcataaac ttataaagga tgtaagggga
     2941 aggggtttaa tgttgggttt agagttcaac cagcctgaag ggggtattct ggataagatt
     3001 accgctggaa gtgttagcaa gataagcagt gaatatcttg gttctcttgt tgccggagaa
     3061 ctcatcaaca ggtatcatat aataaccgca tatacgctga ataatccaaa tgtgatcaga
     3121 ctggaacctc ctcttatagt tacaaaagaa gacctggatg tactggtcaa tgcactggac
     3181 gatcttttta cttctcaccc gaccttcacc agaatagccc ttgcaagtac taagactata
     3241 tttggttcta tctttaaaag ataggtggta aagcatgaaa tcaggtttgg taacgataat
     3301 tggaagacca aatgttggta aatcaacact cttaaatgcc cttatcggag aaaaggtcag
     3361 tatcgtatca gatagaccac agactaccag aaagaacatc cttggtatcg tgagtaagga
     3421 tgactttcag gtaatgtttc ttgatacacc aggtatacat aagcccaagg atgacctcgg
     3481 tgaacatatg gtaaaagatg caatatcagc actggaggat gatgttgatc tgataatctt
     3541 tattgtagat gcaaaagaga ttgccgatga ggatatctac atagataaat atgtacttcc
     3601 aaagtatcaa aacaaaaaga tcctggctat aaataaatca gaccttgtta ataccgaccc
     3661 gatagagagt gtaagaaata agattaatat ctccaattat gacgaggtta tccccatatc
     3721 tgctctgtat gggacaaatc ttgataagct agaagaagca ataaagaagt accttcccga
     3781 aggaccgcag tactatccag aagatgttaa aacaacagaa ccgatagagg ataggataag
     3841 ggagataata agggaaaaag tcatactact tacatatcag gagatacctt acagtatagc
     3901 ggtggtgctg gaagagtttg aggagagaaa tgatttattc tatataaggg cggtgatatt
     3961 tgtagagaag gagtctcaga aggggattat tataggtaaa aatggtaaaa aacttaagga
     4021 gataggtact ttagccagag aagagttgga ggctctgctt ggtaaaaagg tttatcttga
     4081 cctctgggta aaggtaaggg ataagtggcg taagaaggca agatgggttg aggagttagt
     4141 ctacagttaa tttttctatg atataattat ctctatgaat tcttattata ttactggtat
     4201 tattctctcc catgaaaata taggagaaaa ggatagactg attacctttc ttacagaaga
     4261 gaggggaaag ttaagggcgg tagccagaag ctccagaaga atatccagca agatggctgg
     4321 aagtttggag ccttttaacc tggtcagatg ctggatgcat actggaaaga cctttgatat
     4381 tatcaagaat gttcagctta taaagacatt tccaaatgta ataaaaaata tagacattta
     4441 tataacggcg agcaggatgt ttgagtttat acaggttacc gttcaagaaa acgatgaaag
     4501 ccaggatatg ttcagacttt tactcggtgt attaaccgct ctggacaggt atgatagtct
     4561 gaactctata aaggtattct tctggctcaa tgtattaatt ctatctggtt acaggatgag
     4621 gctggataga tgtgtctgtg gtagggaaga tgtaaaaggt tttagtccat ctatgggcgg
     4681 agttgtgtgc atcgattgta tgttgaagac aaatgatagt atactcatat ctaattctgc
     4741 cctggacacg ttgagaatgt atgagcagat aagattgagc tcggttgtta aaagagagat
     4801 accggatacc ctgatgagag agattgatac gattattgat agattcaggt tatgccatac
     4861 gaatataaaa ctcaggtcag aagaggtgac tatatgacat ttcaagaagt gatcttgaaa
     4921 cttttgaatt tttggcagga gcataactgc ataattacac agccgtacga tatggaggta
     4981 ggggctggta ctatgagccc ggatacattt ttcagggctc tgggtagaga aaaatggaat
     5041 gttgcttatg tccagccaag tagaagacca gcagatggca ggttcggaga gaatccaaat
     5101 agactcggta aatactatca gcttcaggtg atattaaagc cagccccaaa taatgtgcag
     5161 gatatctaca tagatagtct gaggtctctc ggta
//
LOCUS       DAOTKU010000056         5166 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_14269_length_5166_cov_3.161025, whole genome shotgun sequence.
ACCESSION   DAOTKU010000056 DAOTKU010000000
VERSION     DAOTKU010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5166
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14269_length_5166_cov_3.161025"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /locus_tag="P5310_02260"
     CDS             complement(<1..842)
                     /locus_tag="P5310_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32220.1"
                     /translation="MDIPDYIKIINELKEGELSHIYILSGDWDFLKEDLISKIFDLLR
                     KKGDTFIVHTESEIDLEKEIKNARNVLIPTTKLIVAKLNSTPKLKVIPDDIYLIIDSN
                     SPIRMSKGKLVKLSPSGPRELEGYIKEHIRGLSRYYDKEIDDASVNILTLGLAQSPHL
                     LLSIWNTLLLLGGDKKRIEEEDVREVVKNIPAFTGFQVIDMLERGRVTEALFFLRREY
                     YPDSEIMQLLSMLRKRYKLILSAKVMNPQDLEFARTFNISAGYARFILSISKDYKMED
                     LSKK"
     gene            complement(808..2178)
                     /locus_tag="P5310_02265"
     CDS             complement(808..2178)
                     /locus_tag="P5310_02265"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HRU32221.1"
                     /translation="MWNEIAGRPSVILFLSLVTGISIFRYINLLFVPLIFLAAGFLFI
                     KDKVSVIIILVLLSSWWASKNYNRYMDSIERVKSLSGSRVNAVCRVKDISRYGMVVDN
                     LIIENDKYYGVLDVNTEEKFHHGDTLLISGKIYENRINSVERREFQTRLIGTLYSNRI
                     TLLSSKKFSLKRSLYLLRQKIKDRLSPFFTTEERDLIMAMVLGIDEDLNYEIYKNFKA
                     TGLIHTLVASGAQVSILVAGLIPFIKGGWIAILFLLIILYSFITGLGISIIRAGIMMG
                     INLIARVLNEDYDPFSSLAFSGSLILLFDPFAIFGTSFQLSFLATFSLIGISPLINIK
                     RLNFIIPTLTVQGVLAPLLLYKNGSLPLISFPINVIVAPLISIITIGGFILALSTFTF
                     PFITSIISIIIKPVIYLLLRTVNLGNSMTYNIDYSPGTIELIAMYIIIGGLIYLGYTG
                     LHKDNK"
     gene            complement(2168..2743)
                     /locus_tag="P5310_02270"
     CDS             complement(2168..2743)
                     /locus_tag="P5310_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HRU32222.1"
                     /translation="MTNRERYLLGVIIILVGALLFSLNKPEEKEEQSSSIFIEENTES
                     IKVAVHIGGAVRNPGLYYLDPSSRVADAIQCAGGPTADADLDAINLASKVTDGLKIIV
                     PSRVNSGNELITADSGQPSGTSESGGKININTASAEELEELNGIGKVLAERIVSFRET
                     NGPFKSIEEIKKVSGIGEKKFEAIRDLIVVE"
     gene            complement(2745..5165)
                     /gene="leuS"
                     /locus_tag="P5310_02275"
     CDS             complement(2745..5165)
                     /gene="leuS"
                     /locus_tag="P5310_02275"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HRU32223.1"
                     /translation="MEEKWQRRWAQEGLDKAKDDFSSPKAYILEMFPYPSGDIHMGHV
                     RNYVLGDVIARYHRFKGYNVLHPMGWDAFGLPAENAAIKRGIHPARWTWSNIENMRKQ
                     LKRLGISYDWDRELATCDPTYYKWTEWFFLLLYKRGLAYRGRAKVNWCPTCKTVTANE
                     EVIDGKCWRCHQPVEERYLEQWFFKITDYAERLLNDLDRLPGWPEHVKTMQRNWIGKS
                     VGAEIIFKIKEIDKDVKIFTTRPDTLFGATFFAVSPDSSLINEMKSSPLFRREEVESF
                     ISYVKSATDENERFGVFTGLHAINPVNGEEIPILVANYVLQEYGTGAIMGVPAHDQRD
                     FDFAKKYGLPVKTVILPPGRPPEEELESAYEGEGVLVNSLMFNGLYNEIAKVKIVEYL
                     EKKGLAEPKINYRLKDWLISRQRYWGSPIPIVYCDNCGVVPVPEEDLPVLLPEDADFA
                     PGSISPLAKSEEFVNTTCPKCGGPAKRETDTMATFICSSWYYLRYTDPHNSKMPFGKD
                     AVKYWMPVDRYIGGVEHAVLHLLYSRFFTKVLYDAGYVDFEEPFTNLFTQGMVLKDGV
                     AMSKSLGNIVEPSPIIEKYSADTLRLFILFASPPEKELEWSDEGVDGMWRFLNRIWRL
                     FNELIPYVNNGDDALDSGIERELIRWRHISVKRVTEDIIERFHLNTAISTLMEWSNFL
                     GDNIDAVKKVKGVILRDTLETFLVLLSPFTPHIAEELWEMLGHKESIFREKWPEYDPN
                     LMKQFEYELVVQVNGKLRDKIRTDERDMEKLKYAVLELPKVKRFLQGKEIKNIITVPE
                     KLINIVTD"
BASE COUNT         1424 a         1222 c          915 g         1605 t
ORIGIN      
        1 atttttttac tcaggtcttc catcttataa tccttgctta tacttaagat aaacctggca
       61 tatccagccg agatattgaa ggtccttgca aattccaggt cctgagggtt cataaccttg
      121 gcagagagta taagtttata cctctttctc aacatcgaga gtaactgcat tatctcgcta
      181 tccggatagt attcccttct tagaaagaaa agagcttctg taactcttcc cctctccagc
      241 atatctataa cctgaaaacc tgtgaaggcg gggatgttct ttacaacctc tcttacatct
      301 tcctcctcta tccttttttt atctccgccc agaagtaaaa gggtattcca gatagagagg
      361 agaagatgtg gggactgtgc aagaccaagg gtaaggatat tgaccgaagc atcatctatc
      421 tctttatcat agtacctgga gagacccctt atatgttctt ttatataccc ctccagctct
      481 cttggacctg aaggggatag ttttaccagc ttacctttag acatcctgat aggagagtta
      541 ctatctataa taagatagat atcatcagga ataaccttga gttttggagt actgttaagt
      601 tttgccacta ttaattttgt ggttgggatc agcacgtttc tggcgttttt tatctccttt
      661 tctaaatcta tctctgattc agtatgaacg ataaaggtat cccccttctt cctgaggaga
      721 tcgaatatct tggagattaa atcctccttg aggaaatccc aatcaccgct caatatatag
      781 atatgtgaaa gttccccctc ttttagctca tttattatct ttatgtagtc cggtatatcc
      841 aagatatatc aatcctccaa taatgatata catcgctatg agttctatgg tacctggtga
      901 atagtctata ttataagtca tactgtttcc aagatttact gtacgcagta aaagataaat
      961 cactggtttt attataatgc ttattatcga tgtgataaag gggaatgtga atgtggacag
     1021 ggctaatata aatccgccta tggttattat agagataagc ggagctacta tcacatttat
     1081 cggaaagctt atcagaggta aagaaccatt cttatagagt agcagagggg ctaagacacc
     1141 ctggacagta agtgttggta ttataaagtt aagccttttg atatttataa gtggagatat
     1201 ccctatcaga ctgaaagtag ccaggaaact cagctggaag ctcgttccga agatagcaaa
     1261 agggtcaaat aggaggatga gagagccgga gaatgccaga cttgagaatg ggtcataatc
     1321 ttcgttcaac actctggcga taagatttat acccatcata ataccagctc ttattatact
     1381 tatacctaag ccagtaataa aggaatagag aatgattagc aggaacagta ttgctatcca
     1441 tcccccttta atgaagggta tcagacctgc gacaagtatg gagacctgag cccctgaggc
     1501 aacaagtgta tgtatgagtc cggtagcctt aaaattcttg taaatctcat aattcaaatc
     1561 ttcgtctatc ccaaggacca tagccattat aaggtctctc tcttcggtgg tgaaaaaagg
     1621 actgagccta tcctttattt tctgtcttaa gagatagaga gacctcttca gtgaaaattt
     1681 tttactggag agaagagtta tcctgttaga ataaagggtt ccgattaatc ttgtctggaa
     1741 ttctctcctc tctacggaat ttatcctgtt ctcataaatt ttcccgctga taagaagtgt
     1801 atcaccgtgg tggaatttct cttctgtatt tacatccaaa actccgtagt atttatcatt
     1861 ttctatgatc aagttatcga ctaccatccc atatcttgag atgtctttta ccctgcagac
     1921 agcattaact ctggagccag ataggctctt cacacgttcg atggaatcca tatatctgtt
     1981 atagttttta gatgcccacc aggaactcag caggaccaga attattatca cgctgacctt
     2041 atcctttata aagagaaagc cagctgctaa gaagataaga ggaacaaata atagatttat
     2101 atatctgaag attgatatac cagtaaccag agagaggaat agaataacac tcggacgacc
     2161 agctatctca ttccacaacg atcaaatccc taatcgcttc aaacttcttc tcaccgatac
     2221 cagatacctt ctttatctct tctatactct tgaatggtcc attggtctcc ctgaaagaga
     2281 ctattctctc tgccagcact ttacctatcc cgtttaattc ctccagttct tcagcacttg
     2341 cagtatttat atttatcttt cctcctgatt cagaggttcc agatggctga ccagaatctg
     2401 cagttattag ctcattccca ctatttaccc ttgacggtac tataattttc agcccatcag
     2461 ttactttgct tgccaggttt atcgcatcca gatctgcatc agcggttggt ccacctgcac
     2521 actgtatggc atcagccacc cttgaactgg gatcgagata gtaaagaccg ggatttctca
     2581 ctgcaccacc gatatgaaca gctaccttta tactctcagt attctcttct atgaatatag
     2641 aagagctctg ctcctctttc tcttctggtt tattgagaga aaagagcaga gccccaacaa
     2701 gtattataat tacacctaat agatatcgct ctctattggt caccttaatc cgttacaata
     2761 tttataagtt tctccggtac agtaattata ttttttatct ccttaccctg aagaaacctc
     2821 tttaccttcg gtagctccag tacagcatac ttcagcttct ccatatctct ctcatctgtt
     2881 cttatcttat ccctcagttt tccattcacc tgtactacta gttcgtactc aaactgtttc
     2941 atcaggttgg ggtcatactc tggccatttc tccctgaata tactctcctt atgacccaac
     3001 atctcccata actcctctgc tatatggggg gtaaatggtg agaggagtac caggaatgtc
     3061 tcaagagtat ctcttaagat aactcctttt acctttttca ctgcatctat attatcaccc
     3121 aggaagttac tccactccat caaggtactt attgcagtgt taagatggaa cctctctata
     3181 atatcctctg ttaccctctt tacagagatg tgcctccacc ttatcagttc tctctctata
     3241 ccactatcaa gggcatcatc accattattg acgtaaggta tcagttcatt gaataatctc
     3301 cagatacggt tgaggaatct ccacataccg tcaacaccct catcgctcca ctccagttcc
     3361 ttctcagggg gagaggcaaa gaggatgaat aatcttaacg tatctgcaga atacttctca
     3421 attatcgggg atggctccac tatattcccc agggactttg acatagcaac cccatctttc
     3481 aataccatac cctgagtaaa caggtttgta aacggttctt caaagtcaac atatccagca
     3541 tcgtatagaa ccttggtaaa aaacctcgag tatagaagat ggaggacagc gtgctctact
     3601 ccacctatat atctatccac cggcatccag tattttactg catctttccc gaagggcatc
     3661 ttactgttat gcggatcggt gtatctcaga tagtaccagg aagagcagat aaaggttgcc
     3721 atggtatcgg tctctctctt agctggacct ccacacttcg ggcaggtggt atttacaaac
     3781 tcctctgact ttgccagtgg tgatatgctc cctggagcaa agtccgcatc ctccggtaaa
     3841 aggacaggaa ggtcttcttc cgggacggga accacaccac agttgtcaca gtaaactatc
     3901 gggatgggtg aaccccagta tctctgacgg gagataagcc aatctttaag tctgtagttt
     3961 atctttggtt cagccagccc tttcttttcc agatattcaa ctatctttac ctttgctatc
     4021 tcattgtaca gtccgttaaa catcaaagaa tttacaagaa ctccttcacc ttcatatgcc
     4081 gattcaagtt cttcttcggg aggtctacca ggtggaagta tgacagtctt taccgggagt
     4141 ccatactttt tcgcaaagtc aaaatccctc tgatcatgag ctggaacacc cataatcgcc
     4201 cctgttccat actcctgcaa tacgtaattg gctacaagga taggaatctc ttctccgtta
     4261 acaggattta tcgcatgaag acctgtaaat acaccaaatc tttcattttc atctgttgca
     4321 gactttacat aacttataaa actttccacc tcttctcttc tgaatagcgg agagcttttc
     4381 atctcattta taagactgga gtctggagaa acagcaaaga atgtagcacc gaaaagtgta
     4441 tcaggtctgg tggtaaatat cttcacatct ttatctatct cttttatctt aaatataatc
     4501 tctgccccga cactcttacc tatccagttt ctctgcatag tctttacatg ttcaggccag
     4561 ccaggaagtc tatccaggtc gttcaaaagt ctctctgcat aatcggtgat cttaaagaac
     4621 cactgttcca gatatctctc ctctacaggc tgatgacatc tccagcattt cccatctatc
     4681 acctcttcat ttgctgtaac tgtcttgcag gttggacacc agttcacctt agcccttcct
     4741 ctgtatgcca aacctctctt atagaggagc aggaaaaacc actctgtcca tttatagtag
     4801 gtagggtcgc acgttgccag ttctctatcc cagtcatagg atatcccgag gcgcttcaac
     4861 tgttttctca tattctctat attgctccag gtccacctcg ccggatgtat gcctctcttt
     4921 atagctgcat tctcagctgg aagaccaaac gcatcccagc ccattggatg taaaacatta
     4981 tatcctttga acctgtgata cctggcaata acatctccaa ggacatagtt tctcacatgt
     5041 cccatatgta tgtctccaga cggatatgga aacatctcca atatataagc cttgggagaa
     5101 gaaaaatcgt ccttcgcctt atctaacccc tcctgagccc atctcctctg ccacttttct
     5161 tctata
//
LOCUS       DAOTKU010000057         5165 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_14270_length_5165_cov_3.344227, whole genome shotgun sequence.
ACCESSION   DAOTKU010000057 DAOTKU010000000
VERSION     DAOTKU010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5165
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14270_length_5165_cov_3.344227"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="P5310_02280"
     CDS             complement(<1..479)
                     /locus_tag="P5310_02280"
                     /inference="COORDINATES: protein motif:HMM:NF013335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HRU32224.1"
                     /translation="MKKVIIDTDIGGDIDDALAVALALNSPELDVIGITTVFENTLLR
                     AKLAKKLLQVFNRSDIPVIKGVEKPIINDWDRSLIPPQVRAVREEIEVNENINAVDFI
                     VEKIMNSAEPVTLITIGPLTNIAGAIIKEPGLKDKTRIYMMGGMYSQAFPEWNIYCDP
                     "
     gene            complement(476..1408)
                     /gene="rbsK"
                     /locus_tag="P5310_02285"
     CDS             complement(476..1408)
                     /gene="rbsK"
                     /locus_tag="P5310_02285"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019303 - D-ribose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HRU32225.1"
                     /translation="MNNTILVAGSINMDLVIKVRSIPKPGETISGGTFYRAYGGKGAN
                     QAVTVSRLGEECYFIGRVGKDVFGEELKENLLKNKVNIDYLVEDKDNPTGIAFIMVDN
                     TGENSIVVAPGANSFLSQDNVRRCLPVISKARVLLTQLEIPVETVSFSLELAKKYDIV
                     TILNPAPAKEIDPHILSLADIVTPNESELKTLTGIEPREERDVIRAGRKLLEYGVEIV
                     VVTLGSKGAMMIERDSELYVPAIKVSAVDTTGAGDVFNGALAVRLADGDPVERALKYA
                     VVSAGISVTRRGAQDSIPTMEEVDQFIREEMIEL"
     gene            complement(1448..3055)
                     /locus_tag="P5310_02290"
     CDS             complement(1448..3055)
                     /locus_tag="P5310_02290"
                     /inference="COORDINATES: protein motif:HMM:NF024602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4038 domain-containing protein"
                     /protein_id="HRU32226.1"
                     /translation="MRYVSQNCVIEFSFESGKSYSDPLELSVIFTEPDGEERVVPAFW
                     SRGNIWRVRYSSPKIGTHKFCTVCSDATNIDLHDQTGEIEVISYEGDNPLFKHGPLKI
                     GDNKRYLEHTDGTPFFWLGDTWWMGLCKRLSFGDFQLLTADRVEKGFSVIQIVAGLYP
                     DMPAFDPRGANEAGFPWEKDYSKINPDYFDMADLKISWLVRSGLIPCIVGAWGYHLPW
                     MGIEGMKKHWRYLVARYSALPVVWCLSGEYDMPYYLSKDKEKGRDIQRKGWIEVGKYL
                     RKIDPYHHPVTVHPGTYVREYPDILEVLDFDMLQTGHGDSSIGNTVFRVQQSYKRSPE
                     MPVVNGEVCYEGIGGQCREQIQRLMFWASILNGACGYTYGANGIWQINRKDEPFGSSP
                     HGMSWGNIPWEEAYKLPGSRQLGIAKRLLERYNWWMFEPHPEWVEVETSDENKKNYYY
                     PYSAGISGVVRIVYLPFFYNNFKIREIEEDISYHAFLVNPVDGKEFDVGQVVPEENRE
                     WQLPELVEGSGMRLPVYQDWILVLEAL"
     gene            complement(3074..>5165)
                     /locus_tag="P5310_02295"
     CDS             complement(3074..>5165)
                     /locus_tag="P5310_02295"
                     /inference="COORDINATES: protein motif:HMM:NF039707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6259 domain-containing protein"
                     /protein_id="HRU32227.1"
                     /translation="MDVIVLKNNNLRLELNPSNGALTGLTAVKTGWKILDRPQLGLSF
                     RLLVPIPDRRNNPIYGEKQIVSDVKVSPDSSSAVFIWNSLVSEYGGRHDICLKVEIKL
                     VDQQVLYTLHIDNHSEYVVENVYCPYLGDIQPPLGAEWFKTFLYNYGTAQEWSLWPRY
                     QNMRGYYGIDYPTQFGPPSACGAPTAPFCLLRSADQGLYAGVYEPSSELVAWHTELRP
                     GYGSSIDSRVPEELNIDGKDVAIRFSAVHVPYIQPGESRDLTPLVLQSFEGGWQSGVD
                     IYKEWRNSWMHTPDLPAWVREPHAWQQIHINSPEDELRIPFRDLYKIGKDCAKHGVKA
                     IQLVGWNAGGQDQGNPSHDPDPRLGTFDELKDAIARIQSMGVRVVMFSKFTWADRATD
                     RFRKDLVRCAIKDPYGDYYMHPGYQYQTATQLLDINTKRLIPMCFLNEEYLRICEEEF
                     KKILGLGADGILFDECLHHGPALLCFDTGHGHRYGAPVYANDRKLIQNFARIAHDKPD
                     FLFAGEACYDWEFEAYHLSYHRSESKQHIPLMRYMQPHMPIMTAVTGFDDRNMINQCL
                     MYRYIISYEPYNFKGHLDDFPKTLEYGKKMDALRKELRDYFWDGEFRDTCGAIVTTAD
                     GEIHHPYAVFVNVKNGHLGLVISNYDEYNSAVIKVKLENSYPLEYYRLVDDSQWRSAK
                     WGIKIPPCSAAVVI"
BASE COUNT         1461 a         1325 c          844 g         1535 t
ORIGIN      
        1 gggtcgcagt atatattcca ttctggaaac gcctgagaat acataccacc catcatatag
       61 atccgggtct tatcttttaa tcccggctct ttgattatag ccccggctat atttgtcagt
      121 ggaccaatcg ttataagtgt taccggttcc gctgaattca ttatcttctc tactataaag
      181 tccaccgcat taatgttttc gtttacctcg atctcttccc taaccgccct gacctgtgga
      241 ggtataagac ttctatccca gtcatttata atgggctttt ctaccccttt aatcacaggt
      301 atgtctgatc tattaaatac ctgtaataac tttttagcca gttttgccct taacagtgta
      361 ttttcaaata cggtggtgat accaataaca tcaagctcag gtgaatttaa agccagtgca
      421 accgccaggg catcgtctat atcaccccct atatcggtat ctattatcac ctttttcata
      481 gctctatcat ctcctctctg ataaattgat caacctcttc catcgttggt atggaatcct
      541 gtgctcctct tctggtaact gatattccag cagagacaac cgcatacttc aaggctcttt
      601 ccaccgggtc cccatcggct aatcttaccg ccaatgcccc attgaagaca tccccggcac
      661 ctgtagtatc cactgccgat acctttatag cagggacgta aagctctgaa tccctctcta
      721 tcatcatagc cccctttgac cccagagtta ccaccactat ctcaaccccg tactcaagca
      781 gtttccttcc agcccttata acatctctct cttctctggg ctcaatcccg gttaaagtct
      841 tcagctctga ctcattgggg gtaactatat ctgccagtga aagtatatgg gggtctatct
      901 cctttgccgg cgctggattt aatattgtaa caatatcata cttttttgcc agctccagag
      961 aaaaacttac agtctcgacc ggtatctcaa gctgggtcaa tagaactctg gccttagaga
     1021 ttactgggag acatctcctt acattatcct gacttaaaaa agagtttgcc cccggagcaa
     1081 ccacaatact attttctcca gtattatcca ccattataaa cgctataccg gttggattat
     1141 ctttatcctc taccagataa tctatgttga ctttgttttt tagaaggttc tcctttaact
     1201 cttcaccgaa gacatcttta cctacccttc caataaaata acactcctct ccaagcctag
     1261 atacagttac agcctgattt gctcccttac ctccatatgc cctgtaaaaa gttccaccac
     1321 ttatcgtttc ccccggttta ggaatcgacc ttacctttat caccaggtcc atatttatac
     1381 tgccagcaac caaaatagta ttattcaaat atatccctcc agcatgaagt actcatctga
     1441 tagtatatca cagtgcctcc aggaccagaa tccagtcctg atagacaggt aatctcatcc
     1501 ctgatccttc caccagctcg ggtaattgcc actccctatt ctcctcagga acaacctgac
     1561 cgacatcgaa ctcttttcca tctactggat taacgagaaa tgcatgataa gatatatctt
     1621 cttctatctc tcttattttg aaattgttat aaaagaaagg caggtatacg atacgcacaa
     1681 cccctgagat acctgctgaa tagggataat aataattttt cttattctca tcagaagttt
     1741 ccacctcaac ccattctgga tgtggttcaa acatccacca gttgtatctc tccagtaatc
     1801 tttttgctat tcctaactgt cttgatcctg gcaatttata cgcttcctcc caggggatat
     1861 ttccccagct cattccatgt ggagatgagc caaatggttc atccttccta tttatctgcc
     1921 agatgccatt cgcaccatag gtataaccac aggcaccatt taaaatactt gcccagaaca
     1981 taagcctctg tatctgctct ctacactgac caccaatccc ctcataacat acctcgccat
     2041 ttactactgg catctctgga cttctcttat atgattgctg tactctaaaa acagtattcc
     2101 ctatgctact atcaccgtgc cctgtctgga gcatatcaaa atctaaaacc tctaagatat
     2161 caggatactc tctgacataa gtcccaggat gaacagttac aggatgatga taagggtcta
     2221 tttttcgcag gtatttccca acctctatcc aacccttcct ctgaatgtct ctaccctttt
     2281 ctttatcttt agagagataa tagggcatat catattctcc tgataaacac catacaactg
     2341 gaagtgcaga gtaccttgca accaggtatc tccaatgttt cttcattccc tctattccca
     2401 tccagggaag atggtatccc caggctccaa caatacaggg tataaggcct gaacgaacaa
     2461 gccaactgat cttcagatca gccatatcaa aataatctgg atttatttta gagtaatctt
     2521 tttcccatgg aaaaccagcc tcattcgctc ctcttggatc aaaagcaggc atatcaggat
     2581 agagtccggc aacaatctga atgacagaaa atcccttctc caccctatca gctgtgagta
     2641 actgaaagtc accaaagctc aatcttttgc atagccccat ccaccaggta tcacctaacc
     2701 agaaaaatgg agttccatct gtatgttcca gatatctctt gttatcccct attttaagag
     2761 gtccatgttt aaagagagga ttatccccct cataagagat tacttcgatc tctccagttt
     2821 gatcatgcag atcgatatta gttgcatcag agcagactgt acaaaattta tgtgttccaa
     2881 tcttaggact tgaataacga accctccata tatttcctct agaccagaaa gcaggaacta
     2941 ctctttcttc tccatccggc tcggtaaaga taaccgagag ttctaatgga tcagaataac
     3001 ttttcccgga ttcaaaagag aactcaatca cacagttttg agagacatat ctcatatttt
     3061 gcctcctaca cttttatata actactgctg cagagcaggg tggtatcttt ataccccatt
     3121 tagctgatct ccactgtgaa tcatctacaa gtctataata ttctaacgga tatgaattct
     3181 ccagttttac tttgataact gcactgttat attcgtcata gttcgatatg accaatccta
     3241 gatgtccatt ctttacattg acaaagacag cataggggtg atggatctct ccatcagcag
     3301 tagttacaat agctccacag gtatcccgga actcaccatc ccagaaataa tcccgcagct
     3361 ccttcctcaa ggcatccatc tttttaccat actccaaagt ctttgggaaa tcatccagat
     3421 gtcctttaaa gttgtatggt tcataactta ttatatacct gtacatcaag cactgattga
     3481 tcatattacg atcatcaaag ccagtaacag cagtcataat tggcatatgg ggctgcatat
     3541 atcgcattaa gggtatatgt tgtttactct cactacgatg gtatgataaa tgataggctt
     3601 caaattccca atcgtaacaa gcttcacctg caaaaagaaa atctggctta tcatgtgcga
     3661 tacgggcaaa attctgtata agtttacggt cattagcgta aaccggagca ccataacggt
     3721 gcccatgacc tgtatcaaag cacaacaggg ctggtccatg atgcagacac tcatcaaaaa
     3781 gtataccatc agcccctaac cccagtatct ttttaaactc ttcctcacat atcctgagat
     3841 actcttcatt caagaaacac atggggataa gacgcttggt atttatatcc agcaattgag
     3901 ttgctgtctg gtactgataa ccagggtgca tataataatc accataggga tcttttatag
     3961 cacaccttac caggtctttt cggaatcgat cagtggctct atctgcccac gtaaacttgg
     4021 agaacattac caccctaacc cccatagatt gtatcctagc aatagcatct ttaagttcat
     4081 caaaggtgcc caaacgtgga tctggatcgt gagatgggtt accctgatcc tgtcctccgg
     4141 cattccagcc aactaactga attgccttaa caccatgttt tgcacaatct ttgccaatct
     4201 tatacagatc tctgaatgga attcttaatt catcttcagg ggaatttata tgaatctgct
     4261 gccaggcatg aggttctcta acccatgctg gtaggtctgg agtatgcatc cagctattac
     4321 gccattcctt gtatatatca actccactct gccatccccc ttcaaaactc tgtaacacca
     4381 aaggtgtcag atcccttgat tctccaggtt gtatataagg cacatgaacc gctgaaaaac
     4441 ggatagccac atctttacca tcaatattaa gttcctccgg taccctgcta tctatggagc
     4501 taccataacc tggtctcagc tctgtgtgcc atgcgacaag ttctgaacta ggctcataaa
     4561 cccctgcata tagaccctga tcagcactgc gtaataagca gaagggtgca gtaggtgctc
     4621 cacatgcaga tggtggacca aactgagttg gatagtctat gccataatat ccacgcatat
     4681 tttgatatct tggccacaaa ctccattcct gtgcagtacc atagttatac aaaaatgtct
     4741 taaaccattc agctcctagt gggggttgta tatcccccag atagggacaa tacacatttt
     4801 ccactacata ctccgaatga ttatcaatat gaagagtata cagtacctgc tgatcaacaa
     4861 gcttaatctc aacctttaaa catatatcat gtctaccacc atattcagaa acaagactat
     4921 tccagataaa aactgcactg ctagagtctg gactgacctt tacatcagat actatctgct
     4981 tctcgccata tatcggatta ttcctcctat ctggaatggg aaccagcaga cgaaaagaaa
     5041 gtccaagctg gggacggtca agaattttcc agcctgtctt tacagctgtc aatccagtta
     5101 acgctccatt agacggatta agttccagac gaagattatt atttttaagc actataacat
     5161 ccata
//
LOCUS       DAOTKU010000058         5155 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_14303_length_5155_cov_3.382157, whole genome shotgun sequence.
ACCESSION   DAOTKU010000058 DAOTKU010000000
VERSION     DAOTKU010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5155
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14303_length_5155_cov_3.382157"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..1827)
                     /locus_tag="P5310_02300"
     CDS             complement(4..1827)
                     /locus_tag="P5310_02300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HRU32228.1"
                     /translation="MRALKTFFLSLITVVLLLIIIVSIFTIVVIVQYLPNLPDPSTLL
                     YYNPPVKTTIYDAKAKPLAELYSEKRIIVPAKDIPEYLMDAVVAIEDERFYSHTGIDL
                     RGIARALYRDLKYGELREGGSTITQQLARNVFLTLEKTPSRKIMEILLALRIETTLSK
                     EEILHLYLNIIYFGEGCYGVESASKTYFGKSVKNLNLAECALLAGLIRSPENYSPLKN
                     KEMAISRQKIVLGKMLQLGFIDEEEYERALKTELKFSTSKESLWKAPYFVDYVLYIVK
                     NGLGLKDIEKNGLNIYTTLDLDLQKIAEDVLRSGLEEAKSYNVSQGAIIVIENKTGYI
                     RAMVGGANYKESQFNRAVQAYRQPGSAFKPFVYTVAIEEGWKPDDIIIDEKVSYKVGN
                     KWWTPQNYDRTFRGAMTLEDALAYSINIPAIKLLQKVKVDKVLQLCIEMGLPLDYNLD
                     RNLALALGGITRGVTPLQMAQAFSVWANEGILIKPVAIKVILDREGKTLYKAEVKPSR
                     IIDEDVARTITRMLEKVVEYGTGKRANCGHPCAGKTGTTSDYRDAWFVGFTKQYTTCV
                     WLGNDNNTPMNGVTGGTFPASIWGKFMSQALKGVQPVPLSD"
     gene            complement(1829..3253)
                     /gene="gcvPB"
                     /locus_tag="P5310_02305"
     CDS             complement(1829..3253)
                     /gene="gcvPB"
                     /locus_tag="P5310_02305"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HRU32229.1"
                     /translation="MLERSLFEERKSRSTRYVDPPETELGIPENLIRKTDLKNFPGLS
                     EPEVVRHFTNLSALNYGIDNNFYPLGSCSMKYNPKINEALAELDEFVDIHPYMDEEYA
                     QGALQIMYELERLLSEITGMYEFTLHPSAGAHGELTGLFIAKAYFDDRKERRNLVIVP
                     DSAHGTNPASAAMAGFNVVEVKSDKRGLISPSAIQPYLNEDLALIMLTNPNTLGLFEE
                     DIEEIARLTHNAGGLLYYDGANLNGIIGITRPGDMGFDIVHLNLHKTFSTPHGGGGPG
                     SGSVGVKEYLAPFLPIPRVIHGDNCYTLDYNLPKSIGKIKCFYGNFTVLVKAYCYIRL
                     LGRENLRSIAEMATLNANYLQERLSEILDIPYKRLCKHEFVASLKFLKNRGIRALDIA
                     KALIDKGFHPPTVYFPLIVEEAIMIEPTETESLDTLDRFVEALKEILDTAESDPSLVK
                     ESPRSHRVKRVDEAKASRMLRVRW"
     gene            complement(3231..4571)
                     /gene="gcvPA"
                     /locus_tag="P5310_02310"
     CDS             complement(3231..4571)
                     /gene="gcvPA"
                     /locus_tag="P5310_02310"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF001696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HRU32230.1"
                     /translation="MDYIPHLPEDIRHMLEALGLDSVDQLFSDIPDNLKLNKEIDIPG
                     SINEIELTRIIREISNLNKTSEDFLWFLGGGCYRHFVPQALNEISHRQEFYTSYTPYQ
                     AELSQGTLQTFFEFQTLICDLLQMEVTNDTMYDGATATAEAMFMACSITKKNKVLVGR
                     SLHPEYRMVLNTYAKARGINIVEVPFDGDSGRLSLESLDNLLDDSACLIFQTPNFFGV
                     IEDPDLFEPLVHSRDAFLIPVIVEPLSLALIKPPGEYNADIACGDLQSFGLGMRFGGP
                     SAGFLATSMKYIRRLPGRIVGMTEDAEGRVSYAFVLQTREQHIRRASATSNICSNEAL
                     CAIKVAIYLSLLGKDGLKDLALLNHHLADLAYKELGSYFDGPFFNEFLISLDSPSSKV
                     RNILKDKNILTLDISRWYPELDRSILVTFTEINRKDDIYTLIEEIKPYVRKISL"
     gene            complement(4571..4948)
                     /gene="gcvH"
                     /locus_tag="P5310_02315"
     CDS             complement(4571..4948)
                     /gene="gcvH"
                     /locus_tag="P5310_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015169940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HRU32231.1"
                     /translation="MIPEDIKFTDTHEWIRIDGEYGIIGVTQYGFEKLADVVYIELPE
                     IGTRIKQGEPFGVIESVKAAVDLIAPVSGIVEDVNESARSSPETLPDDPYGESWLIKV
                     KIEDPSEVEKLLSSAAYEELIRE"
BASE COUNT         1489 a         1156 c          886 g         1624 t
ORIGIN      
        1 tacctaatca gagaggggaa ctggctgaac accttttaac gcctgactca taaatttccc
       61 ccatatagat gctggaaatg tccctcctgt tactccattc ataggtgtat tattatcatt
      121 tcccagccat acacaggtag tatactgttt ggtaaaacca acaaaccagg catccctgta
      181 atcactggta gtccctgtct ttcctgcaca gggatgacca cagttagccc ttttacctgt
      241 cccatattcc accactttct ccagcattct ggtaattgtc ctggcgacgt cttcatctat
      301 aatccttgaa ggttttacct cagccttata gagagtttta ccctccctat ccaggataac
      361 cttgatagct acaggtttaa tcaaaatacc ttcgttagcc cagacagaaa aagcctgagc
      421 catttgtaaa ggggttaccc cccgggttat acctcctaaa gctagggcta gattcctatc
      481 taggttgtag tctaaaggta agcccatctc tatacagagt tgcagcacct tatctacctt
      541 taccttctgt aataatttta tggcaggtat atttatagaa tatgccagcg catcttctaa
      601 tgtcatagct cctctaaagg tcctgtcata attctgaggt gtccaccatt tattaccgac
      661 cttgtaactt accttctcat cgattattat atcatcaggt ttccatccct cctcaatcgc
      721 cacagtatat acaaagggct taaaagcaga cccaggctgt ctataggcct gtaccgctct
      781 gttgaactgg ctttctttat aatttgcccc acctaccata gcccttatat agccggtctt
      841 attctctata acgattattg ctccctgaga tacattataa cttttagctt cctctaaccc
      901 agaccttaat acatcctcag ctattttctg caggtctaaa tctagtgttg tatatatatt
      961 caatccattt ttctctatat ctttaagccc cagaccattc ttaacaatat ataatacata
     1021 atctacaaag taaggggctt tccataaact ttccttacta gttgaaaatt tcagttctgt
     1081 tttaagtgcc ctttcatatt cttcttcatc tataaatcca agctgaagca tctttccgag
     1141 gacaatcttc tgcctggata tagccatctc tttattttta agtggagaat aattctctgg
     1201 agacctgatc aaaccagcca ggagggcaca ttcagcaaga ttgaggttct ttacagactt
     1261 tccaaagtat gtttttgatg ccgattctac cccatagcag ccttcaccaa aatatattat
     1321 attcaaatat agatgcagga tctcttcttt ggatagagta gtttctattc taagtgctag
     1381 aagaatttcc attatcttac gggatggcgt tttctccagg gttaaaaata catttctggc
     1441 aagctgttga gtgatggtac tgccaccttc tctaagttca ccatatttta agtccctgta
     1501 cagtgctctt gctatccctc ttaagtctat cccggtgtgg gaatagaacc tctcatcttc
     1561 aatagcaact accgcatcca taagatattc agggatatct tttgccggga ctattattct
     1621 tttttcagaa taaagctctg caagaggctt agccttagca tcatagattg ttgtttttac
     1681 aggtggatta tagtatagaa gagttgacgg atcaggtaag tttggaagat actgaactat
     1741 aactactata gtaaatatgg agacaatgat gataaggaga agaacaacag taatcagaga
     1801 gaggaaaaat gtctttaatg ccctcatatt accatctgac tcttaacatt ctggatgcct
     1861 ttgcctcgtc tactcttttt acccggtgac ttctgggaga ttcttttaca agagacggat
     1921 cagattctgc agtatccagt atctctttta aagcctcgac aaatctgtcc aatgtatcta
     1981 gagattctgt ctctgtaggt tctatcataa tggcttcctc tactattaga ggaaagtata
     2041 cggttggtgg atggaaccct ttatcaatca gagctttagc gatatctaat gcccttatcc
     2101 ctctattttt gagaaatttc aaagaggcta caaactcatg tttacacagc cttttataag
     2161 gtatatccag aatttcactt aacctctcct gtaaataatt ggcatttaaa gttgccattt
     2221 cagctataga cctcagattt tctcttccaa gcaaccttat ataacaatac gccttgacca
     2281 gaacggtaaa atttccatag aaacatttta tctttcctat agactttgga aggttgtaat
     2341 ctaacgtgta acaattatca ccgtggataa ctcttggaat cggtaaaaac ggagcaaggt
     2401 attcttttac ccctacagat ccagaccccg gtcctcctcc accgtgaggt gttgaaaatg
     2461 tcttatgtaa attcaaatga acaatatcaa accccatatc gccaggcctg gtaataccga
     2521 ttattccatt aagattcgcc ccatcatagt ataaaagccc gccggcatta tgggttaacc
     2581 tggctatctc ttctatatcc tcttcaaata aacccaaggt atttgggttt gtaagcataa
     2641 taagggcaag gtcctcattc agataaggtt gaatagcaga aggagagata agccctcttt
     2701 tatctgactt cacttctacc acattgaaac cagccatagc agcactggcg gggttagtac
     2761 catgtgcaga atctggaact attaccaggt tccttctctc ttttctgtca tcaaagtaag
     2821 ctttagctat gaagagtcct gttaactcac catgggctcc tgcagatggg tgaagagtaa
     2881 actcatacat acccgttatc tcagatagca gtctttcaag ctcatacata atctgtaatg
     2941 ctccctgagc atactcctca tccatataag gatggatgtc tacaaactca tccagctcag
     3001 ctaaagcctc atttattttg ggattatatt tcatagagca agagccaaga ggataaaaat
     3061 tattatcaat tccatagttc agagccgata gatttgtaaa atgcctgacc acttcaggtt
     3121 cactcagacc tgggaagttt tttaaatctg tcttccttat aagattctca gggataccta
     3181 attcagtttc aggtgggtct acatatctgg ttgatcttga ctttctttcc tcaaagagag
     3241 atctttctaa catatggctt aatctcctct ataagagtat atatatcatc ttttctgtta
     3301 atctcagtaa atgtaaccag aatagacctg tctaattcag gataccacct tgagatatca
     3361 agggttaata tattcttatc tttcaagatg ttcctcacct tagaggatgg actgtccaga
     3421 gagattaaaa actcattgaa aaaaggacca tcaaagtaac tcccaagctc tttatacgca
     3481 agatcagcca gatgatgatt gagcagtgct aaatctttca atccatcctt cccaagcaga
     3541 gataaatata tggcaacctt tatcgcacag agtgcttcat tagagcatat atttgaggta
     3601 gctgaagcac gtcttatgtg ctgttctctt gtctgcagga caaatgcata tgatactctt
     3661 ccttcggcat cttcagtcat ccctactatc ctgcccggta gtctccgaat atacttcata
     3721 ctggtagcca gaaagcctgc cgaaggacct ccaaatctca tacccagacc aaatgattgc
     3781 aggtcaccac aagctatatc cgcattatac tctccagggg gctttatcag ggcaagtgac
     3841 aatggttcaa ctattaccgg tataagaaag gcatctctac tgtgaaccag tggttcaaac
     3901 aggtccgggt cctctatgac tccaaagaaa tttggagtct gaaaaatgag acaggcagag
     3961 tcatctaaaa ggttatcaag agattccaat gataatctcc cagaatcccc atcaaaaggt
     4021 acttccacga tatttattcc tctggctttg gcgtacgtat tcagaaccat cctgtattca
     4081 ggatgcaagg acctgcctac cagtacttta tttttctttg taatgctgca agccataaac
     4141 atcgcttctg cagttgcagt ggctccatca tacatggtat catttgtcac ttccatctgc
     4201 aaaagatcac agatcaacgt ctgaaattca aagaaggtct gtaatgtgcc ttgacttaat
     4261 tcagcctgat atggagtata tgatgtgtag aactcctgtc tatgactaat ttcatttaat
     4321 gcctgtggga cgaaatgtct atagcatccc ccacccaaaa accatagaaa atcctctgac
     4381 gttttattta agttacttat ttctcttata attctagtca gctctatctc gttaatactt
     4441 ccaggtatat ctatctcttt attgagtttt agattatccg gtatatccga gaaaagttga
     4501 tcgacggaat ccaaacctaa agcttcaagc atatgtctta tatcttccgg gagatgtggg
     4561 atataatcca ttactctctg attagctcct cataagcggc agaagataga agtttctcaa
     4621 cttcagatgg atcttcaatt ttaaccttta taagccagct ctctccatag ggatcatcag
     4681 gaagggtctc cggtgaactc ctggcagact catttacatc ttcaactata cctgaaactg
     4741 gagctataag atcaacagct gcttttaccg attctataac accgaatggc tctccctgct
     4801 ttatcctagt ccctatctca ggcaattcaa tataaaccac atcggcaagt ttttcaaaac
     4861 catactgagt aacacctatt attccatatt caccatctat ccttatccac tcgtgagtat
     4921 cagtaaactt aatatcctct gggatcataa ttaatcctcc tagtcaatca ttttctttac
     4981 agagataatt ataacatatc agcatctttt agatgaagct tgcgtatctt gccaaaaaac
     5041 agggtgtgct atataattca ctcgtggggt ggggattttt agaagaggaa gggggtgaga
     5101 tgagatgata tacaactgga taaatgttat aattggcata ttaactataa tagta
//
LOCUS       DAOTKU010000059         5066 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_14570_length_5066_cov_3.570545, whole genome shotgun sequence.
ACCESSION   DAOTKU010000059 DAOTKU010000000
VERSION     DAOTKU010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5066
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14570_length_5066_cov_3.570545"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..898
                     /locus_tag="P5310_02320"
     CDS             <1..898
                     /locus_tag="P5310_02320"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HRU32232.1"
                     /translation="KDPTIITLGVFDGVHKGHQAILSLLSEMSLTSGLPGVVITFDPH
                     PLEIVSKSKVELILDLNDRLSIIDQFPHSFNVLINFDSKFAEKSPEDFVKELAEKFNI
                     REIIIGYNFQFGKGRRGDIRFLEEAGKKYKFRVMAVPPVEYNGSSISSSRIRCTLKDG
                     DINSANEMLGRPFYLKGRVIRGRGLGKEIGFPTANISVPQRLIIPKPGVYAVIGTVEE
                     KKFMGVTNIGSAPTVRRESSITVETHFINFNQNIYDKLVKLDFIERIRPEIKFDGIDQ
                     LVLQIRRDIETAEEIIKRKVLT"
     gene            complement(989..1236)
                     /locus_tag="P5310_02325"
                     /pseudo
     CDS             complement(989..1236)
                     /locus_tag="P5310_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495403.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein"
     gene            complement(1290..1655)
                     /locus_tag="P5310_02330"
     CDS             complement(1290..1655)
                     /locus_tag="P5310_02330"
                     /inference="COORDINATES: protein motif:HMM:TIGR02530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02530 family flagellar biosynthesis protein"
                     /protein_id="HRU32233.1"
                     /translation="MVRRIEDVDSRINQVTKSIQNSTDVSFRDVLESKLKTGEINISK
                     HAQERILLRKIEISQEEINRINKAIDLANSKGVRDSLVIINGKAFVINVPTRTVVTAI
                     EEESLKDRIFTNIDGAVIL"
     gene            1709..2536
                     /locus_tag="P5310_02335"
     CDS             1709..2536
                     /locus_tag="P5310_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32234.1"
                     /translation="MGLIENVSIISGDGAPVESCSKTYGKKLCDCSKNGIKYCDCKRK
                     FSDHSARWGRDSYHRHDSVSAVVALAQLKTFYPKLLFSYFVSDSACDKFATYELCKSY
                     NLRPFIDLNHKNTPADGIVKFNGNGIPICPLGLAMSHWGYNKNRCRIKWRCPISASKK
                     KKEKFGECPIRDTCPSSSYGRVVYTYPESNPRLFTTPPRGSNFWREIYTIGRSSAERG
                     IKRILVDYQLERARVRSRKQWYWRILLAGVNIHLDAWLDYRRLNIVGHLTSWAEEAA"
     gene            complement(2821..3222)
                     /gene="flgC"
                     /locus_tag="P5310_02340"
     CDS             complement(2821..3222)
                     /gene="flgC"
                     /locus_tag="P5310_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HRU32235.1"
                     /translation="MSLLNSMNISASGLTAQRLRLDLISSNMANIETTRTEDGGPYRR
                     QVPIFREILDQYTGMTNGVEVAAIIRDNSPFRMVYDPGHPDANLEGYILYPNVNPVVE
                     MTDMISATRSYEANLSLITAAKSMFLKALEI"
     gene            complement(3219..3575)
                     /locus_tag="P5310_02345"
     CDS             complement(3219..3575)
                     /locus_tag="P5310_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein"
                     /protein_id="HRU32236.1"
                     /translation="MVDKTILDTIEAALNAAELRHKVYSANIANVDTPGYKKYRISFE
                     EKLQDALKNNNDLLEVQPEIFRITQTSFRNDGNNVNLDEEIVLMNQNTLKYQALSQLA
                     QWFFDRYYTVIGGRRR"
     gene            complement(3600..4040)
                     /locus_tag="P5310_02350"
     CDS             complement(3600..4040)
                     /locus_tag="P5310_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HRU32237.1"
                     /translation="MSLRLSTKTRYGLRAIIDLAKYYGKGPVMARAIAEEEGISENYL
                     EQIMDPLKKSGLVRSVRGARGGFVLAKPPGNIRVKEVIEALEGPVLLVDCLYDPNLCE
                     RSKSCIARLFWERLRDNIVDFLNSWTLEDFISKGYSGKNQAVDF"
     gene            4370..4789
                     /gene="fucU"
                     /locus_tag="P5310_02355"
     CDS             4370..4789
                     /gene="fucU"
                     /locus_tag="P5310_02355"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HRU32238.1"
                     /translation="MLKGISPLISPELLKILCEMGHGDEILLADANYPAESCGPKVVR
                     ADGIDIPSLLKGIMDLFPLDTYVNENVILMSPNEGKPSIWEKYEEILKASGEDVRIGT
                     LSRFDFYERASKAYAVVATGERALYANIILKKGVIGL"
     gene            complement(4811..>5066)
                     /locus_tag="P5310_02360"
     CDS             complement(4811..>5066)
                     /locus_tag="P5310_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32239.1"
                     /translation="IILYFMLFSIKNLLFLDIEYYHYSMESYHDIQFVTDLQRGVLTN
                     FYNCISESYYRYTNSFITYQSKLGKYEVKIEFTGMRHFRH"
BASE COUNT         1555 a          995 c          950 g         1566 t
ORIGIN      
        1 aaaagacccc accattatta ctctgggtgt atttgatggg gttcataaag gacatcaagc
       61 catactatct cttctcagtg agatgtccct tacttcagga ttgcctggag ttgtaattac
      121 tttcgatcct catccactag aaatagtaag taaaagtaaa gtagaattga tattagactt
      181 gaatgaccgt ctatcgataa tcgaccaatt ccctcattca tttaatgtcc ttatcaattt
      241 tgacagtaaa tttgcagaga aatctccaga agactttgtg aaggaacttg cagaaaaatt
      301 caatataaga gaaatcatta tcggatataa tttccagttt ggtaaaggaa gaagaggaga
      361 tataagattc ttagaagagg caggtaaaaa gtacaaattt agggtcatgg cagttcctcc
      421 tgtagagtat aatggaagct caattagcag ttcaagaata agatgtactt taaaagacgg
      481 agatataaat tctgcaaatg agatgctggg aagacccttc tatctcaagg ggagggtgat
      541 caggggaaga ggactgggaa aagagatagg gttcccgacc gcaaacatat ctgttcctca
      601 gagactaatc ataccaaaac caggtgtcta tgcagtcatc ggcacagtag aagagaaaaa
      661 atttatggga gttaccaata ttgggtctgc cccaactgta aggagagaaa gcagtataac
      721 agtagaaact cactttatca attttaacca aaatatctat gataaactgg taaagttaga
      781 ctttatcgaa cgtataagac cagagatcaa atttgatggc atagatcaac tggtcctgca
      841 gataagaaga gatatagaga ccgcagaaga gatcataaag agaaaagtac tgacgtaagt
      901 tcgatggcat agggcgccaa acttgcgcta ggagggtacc tataaaatga cacctgggag
      961 gtttacacca gtttcttgac agccccttac caggagtata tatagtattt ataccaccaa
     1021 tagtcatacc aagacccacc tgaatagggt ctgtcccccc atacttccct ggggagcaga
     1081 agtccctttc aacgtctgat agaaaaggtc ctggaaagta atcctggcca tcttataacc
     1141 ggtagtattt acattagcta tattattagc taccacatcc atccttacct gatggtttct
     1201 taaaccagaa acaccggcaa acaatgaacg catcatagtt taataatcct ccttttagat
     1261 ggctccctct cagaggtcca gcccaaattt tataatatta cagctccatc tatattggta
     1321 aatattctat cttttaaaga ttcctcctca atagcagtca caacagtcct ggttggtaca
     1381 tttataacaa aagccttgcc atttattata accagagaat ctctcactcc ttttgaatta
     1441 gctagatcta tagccttatt tatcctattt atctcctcct gacttatctc tatttttctc
     1501 agaaggattc tctcctgggc atgtttggag atattaatct ctcctgtttt cagttttgac
     1561 tccaagacat ctctgaagct tacatctgta ctattctgaa tacttttcgt tacctgattt
     1621 atcctgctat ctacatcttc tatccttcta accatctgat atcacctccg atctcttttc
     1681 taaaattgct gtggaacctt ctattaaaat gggtctaata gaaaatgttt ctattatctc
     1741 tggtgatgga gcccctgtcg agtcttgtag taagacttat ggtaaaaagt tgtgtgattg
     1801 ttctaagaat ggtatcaaat attgtgactg caagagaaaa ttctctgacc attctgccag
     1861 gtggggccgg gatagttatc atagacacga tagtgtcagt gctgtagtag ctttggctca
     1921 actaaagact ttttatccaa agcttctctt ctcttacttc gtctcagact ctgcctgtga
     1981 taagttcgct acctacgagc tctgtaaatc ctataacctt agacctttca ttgacttgaa
     2041 ccataaaaat actccagctg atggaatagt taagtttaat ggcaatggca tccctatctg
     2101 tcctcttggt ttagctatgt ctcactgggg ttataataag aaccgttgta gaatcaaatg
     2161 gagatgtcct atatcagcca gtaagaaaaa gaaggagaag tttggagaat gtcctataag
     2221 agatacctgc cctagttcta gctatggtag ggtcgtgtat acatatcctg aaagtaaccc
     2281 caggctgttc actactccac caagagggtc taacttctgg agagagattt atactattgg
     2341 taggtcttca gcagagagag gaataaagcg aatcctggtt gactatcaat tagaaagagc
     2401 aagagttcgt agcaggaagc agtggtactg gaggatatta cttgcaggag taaatatcca
     2461 cttagacgcc tggttagatt atagaaggct taatatagtt gggcatctta ccagttgggc
     2521 tgaagaagca gcttagactt taggctaaat tgcaagacta cagagaaata tactgcaagg
     2581 taaatcttgg gatacatatc ctattgtcca gttttgccct tttgtcctta agacagctta
     2641 tttatctcta tattcatctg tcaaggtgca cctaccttac ccttcaaacc tcaatacact
     2701 gtaaacctgt ttatatttta ccccccacat ttctgtcaag ggggtttccg agaacctata
     2761 ttagctattg gatcaacggt aaaccttaca ggctctatag tattcataaa ttgccctcac
     2821 tcatatctct aacgccttaa ggaacatact cttggcagca gttataaggg acagattagc
     2881 ctcataactc ctggtagcag aaatcatatc agtcatttct accactggat ttacgtttgg
     2941 atataagata tatccctcca gatttgcgtc tggatgcccc gggtcataca ccattctgaa
     3001 cggagaatta tctcttatta ttgcagcaac ctctacacca ttagtcatac ctgtatattg
     3061 atccagtatt tctctaaaga taggaacctg tcttctgtat ggtccaccat cttctgtcct
     3121 ggttgtctca atattcgcca tattacttga tataagatcg agccttagcc tttgagcggt
     3181 aagaccagag gcagagatat tcatactgtt taaaagactc atcttctcct acctccaatc
     3241 acggtataat atcgatcaaa aaaccattga gctaattgtg ataaagcctg atacttgaga
     3301 gtattctggt tcataagtac aatttcctca tcaaggttta cattattccc atcatttcta
     3361 aaagaggtct gagtaatcct gaagatttct ggttgcacct ctaaaaggtc attgttattc
     3421 ttcaacgcat cttgtaactt ttcctcaaag gaaatacgat atttcttata gccaggagta
     3481 tccacattgg ctatattggc actgtagact ttatgtctaa gttctgctgc atttaaagca
     3541 gcttcaattg tatcaaggat agtcttatca accatagcac aaatatacca ctataatatt
     3601 caaaaatcaa cagcctggtt ttttccggaa taaccttttg agataaagtc ttcaagtgtc
     3661 caggaattta agaaatcaac tatgttatct ctaagtcttt cccagaatag acgggcaata
     3721 caactcttac ttctttcaca caaattgggg tcgtacagac aatcaacaag taatacaggt
     3781 ccttctaagg cttctattac ctcttttacc ctgatattac ctggaggttt tgccaatacg
     3841 aaacctcctc tcgcacctct tacacttctt actaatccac tcttcttcag cgggtccata
     3901 atctgttcta gatagttctc tgaaattcct tcttcttcag ctatagccct tgccattacc
     3961 ggtcctttcc catagtactt tgctaaatcg atgatagctc tcaagccata ccttgttttt
     4021 gtagataatc ttaaagacac tctttcacct cttacaaata ttagttatct ggttaagatt
     4081 atagataata ccatatttaa agtcaataga aaaaaccgtg acgcgtaaca ggtaatacgt
     4141 gacaggtaac aagcaatacg tgatgcatta caggtgacag gtcacaaata gaattaatct
     4201 gatatatttt atcgtcctct cccttgacgg gaggaagaaa agggtcgatt ctccgagata
     4261 agggaggtgg aattggctat ttcctcagat agatgatatt taagataatt ctcagttttg
     4321 attatatgtt aaaatattat ttgaattctt agatggaggt gactgtttta tgcttaaagg
     4381 aatttctcca ttaatatctc cagagttgct taagattctt tgtgagatgg ggcatgggga
     4441 tgagatttta ctggcggatg cgaattatcc tgctgagtcc tgcggtccaa aggttgtaag
     4501 agcagatggg atagatatac cttcgcttct caagggcatt atggaccttt ttcctctaga
     4561 tacctatgtt aacgaaaatg tgatattaat gtctcccaat gaagggaaac catcaatatg
     4621 ggaaaagtat gaagagattc ttaaagcctc aggagaagat gtaaggattg gtacattatc
     4681 taggttcgat ttttatgaaa gagcatcgaa agcctatgct gtggtggcta ccggcgagag
     4741 ggctttatac gccaatataa tattaaaaaa gggagtgata ggtctgtagg ctgatgatat
     4801 agatttactt ctaatgtctg aagtgtctca taccggtaaa ttcaatcttc acctcatatt
     4861 tacctaactt tgactggtaa gtaataaagg aattagtata cctataatag gattcagata
     4921 tacaattgta gaagtttgtc aggactcctc tctgaagatc agtcacgaac tgaatatcat
     4981 gatacgattc catgctataa tgataatatt ctatatctag aaagaggaga ttttttatgg
     5041 agaatagcat aaagtacaaa attata
//
LOCUS       DAOTKU010000060         5045 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_14622_length_5045_cov_3.851904, whole genome shotgun sequence.
ACCESSION   DAOTKU010000060 DAOTKU010000000
VERSION     DAOTKU010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5045
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14622_length_5045_cov_3.851904"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="P5310_02365"
     CDS             complement(<1..237)
                     /locus_tag="P5310_02365"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HRU32240.1"
                     /translation="MRILAVGAHPDDLELLCAGTLAKYSKEGHTVVMAHMCNGNKGHF
                     TIPPEELASIRAEEAKKSAEIIGAEIIQGEYPDLE"
     gene            complement(383..2332)
                     /locus_tag="P5310_02370"
     CDS             complement(383..2332)
                     /locus_tag="P5310_02370"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRU32241.1"
                     /translation="MRSSARLLISLMVVFSLIVSISPVSFSQSKVMPGDQYNLSDYQK
                     LTGTKITKFNEAPALAELVKQGKLPSVEKRLPEDPLVVVPYEEIGQYGGTLRGEAHLG
                     MGDKTGWWRMAHEPLVYWDCAHRKIHPNLAKSWKVSDNGKTFTFYIRKGLKWSDGQPF
                     TADDIMFYYEDILLNKELTPTFPSWLVMKGKQVTVEKIDDYTVRFRFQDPYGYFVGRV
                     AEGQDLYAPKHYLKQFHPKYTPKEKIEELAKKEGLNNWYQLFAQKSDWALNPELPEMS
                     AFKTTNLLSSNYHIAERNPYYFKIDPAGNQLPYIDRVRRELVSDIQMMVMKIIAGEID
                     FQLRHIWSLYDTYPLFMENRTKGDYRIIKYTGSITSTGAIFVNQNVKDPVLRSLFQNK
                     MFRRALSLGINREDINQLVLSGLGEPGQGLLPKLHPAYVEKVDKAYTEYDPDKANRIL
                     DSLGLTKRDRDGFRLRPDGKTLAVTIDASTHHTPSIDIMQLVVEQWKKLGIKAEVKVM
                     ERSLFETRHQGNEQELHTFTVSFGADGYSGDRAPNTRWCPLWYNWLNSGGKTGEEPPK
                     EVKKLWQLMNEDFYSTTSEARRTAILKEAMRLHADNLWVIGTAGVNWMMGIARNNLRN
                     VPDSGFTWHPAAPGVFRPEQFFFKK"
     gene            complement(2413..3696)
                     /locus_tag="P5310_02375"
     CDS             complement(2413..3696)
                     /locus_tag="P5310_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRU32242.1"
                     /translation="MRKFMNLFKKEIKELISAQLILSLAFTVILFYFIGNMAKTEMNK
                     IGGAKDIYILNLDDSDLSGEILRNLALANFKINLLDVKDRESAVDFARKNNINLLLII
                     PEGFGPSVLKFQPEELEVYSFIRSFSVSTMSDTEIVKQLIGIVNNYLSDNLIRNRISD
                     ITPEDLKNPIKSRNFIVIKDRMAEGSVTDVANFVYFQSIFIPIILMMVITFSSQMVLS
                     AIAMEKQDKTLETLLTVPISRNYIVMAKMFASGLIGLLSAGIYMIGFRFYMGGFTGSL
                     PSNTQIEGVIDKLGLRFTSSSYIFLGISLFLAILCALAIATILGALTDDLKSAQSAFL
                     PLMFLVLIPYFISIFSDINSLSLPLKILILAIPFSHPFLASQNLLIGNYHIVFYGILY
                     MSGIFVILIFIAGKIFSTDRILTMKLRFRGRRSGS"
     gene            complement(3693..4415)
                     /locus_tag="P5310_02380"
     CDS             complement(3693..4415)
                     /locus_tag="P5310_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRU32243.1"
                     /translation="MDVILDVENLKKSYGKVSALKGIDFSVSTGEVFALIGPNGAGKT
                     TALRIVATLLSPDDGRVNFMGYDVKKNPERVRENISYLPEEAGAYKNLTGIEYLRFMA
                     NFYARDEREVEGFIRRAEEIAELGDRLKDKIGTYSKGMVRKLLLARALMTMPELAILD
                     EPTSGLDVINSLEIREIIRGFVKEGISVLLSSHNMLEIEFLSDRVAIIDSGEILEIGS
                     PEELKEKYSVRNLEEVFRRLVR"
     gene            4562..4882
                     /gene="rplU"
                     /locus_tag="P5310_02385"
     CDS             4562..4882
                     /gene="rplU"
                     /locus_tag="P5310_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HRU32244.1"
                     /translation="MYAIIETGGKQYKVSPGDILTVEKLDIEEGEEVVFDKVLMLKAQ
                     EGESVIGTPYIENIKVRGIVIEQFRGKKIIVFKYRPKKHYRRKRGHRQYLSRVKIEEV
                     LYGS"
     gene            4872..>5045
                     /gene="rpmA"
                     /locus_tag="P5310_02390"
     CDS             4872..>5045
                     /gene="rpmA"
                     /locus_tag="P5310_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HRU32245.1"
                     /translation="MAHKKGAGSTSNGRDSSPKYLGVKRSDGQFVVAGNIIVRQRGTR
                     IHPGQNVGIGKDFT"
BASE COUNT         1441 a         1085 c          903 g         1616 t
ORIGIN      
        1 ctccaggtcg ggatattctc cctgtattat ctctgcccct attatctctg ctgatttttt
       61 tgcctcttcc gctcttatac tggcgagctc ttcaggtgga atggtgaagt gtcctttatt
      121 cccattgcac atatgagcca taactactgt atgtccctct ttactgtact ttgccagagt
      181 tcctgcacat aacagttcca gatcatctgg atgagcccca accgcaagaa ttctcactgt
      241 aaatcacctt ctttctaaaa atcaaacctg ataaaattat actacattta ataatttttt
      301 tcattcctag aggtagctgg tctataaaaa atatagccct ggcaattatt tgccagggct
      361 ttttattcat cagaaggttc ttttatttct taaagaagaa ttgctcaggt ctgaagaccc
      421 caggagcggc aggatgccat gtaaaaccag agtcaggaac atttcttaag ttgttccttg
      481 ctattcccat catccagttt acaccagccg tccctataac ccagaggtta tctgcatgta
      541 acctcattgc ttctttcagt atcgctgttc ttctcgcttc acttgtggtg gaataaaagt
      601 cttcattcat caattgccag agctttttta cctctttagg tggttcttct cctgtttttc
      661 ccccagagtt caaccagttg taccataacg gacaccacct ggtattggga gctcgatctc
      721 cagaatatcc atctgctccg aagctgacag taaatgtatg taactcttgc tcattgccct
      781 gatgccttgt ctcaaagaga gacctttcca tcacttttac ctctgccttt atacccagtt
      841 tcttccactg ttccaccacc agttgcataa tatctatgct gggtgtatgg tgggtagatg
      901 catctattgt gacagccagt gtttttccat ctggacgtaa tctgaagcca tccctatctc
      961 tctttgtcag ccccagggag tccagaattc tgttagcctt gtccggatca tattctgtat
     1021 atgccttatc caccttctct acatacgctg gatgcagctt gggtaagagt ccctgcccgg
     1081 gctctcccag tccagagaga acaagttgat ttatatcttc tctattaata cccaacgata
     1141 gtgcccttcg gaacatctta ttctggaata gtgaccttaa aacagggtct ttgacgttct
     1201 gattgacaaa tattgcaccc gtacttgtaa tacttccggt atactttatt atcctgtaat
     1261 ctcctttggt tctattttcc ataaagagag gataggtatc atataaactc catatatgcc
     1321 tcagctgaaa atctatctct cctgctataa ttttcattac catcatctga atatctgaga
     1381 caagctctct tctcaccctg tctatgtatg gcagctgatt cccagccgga tctatcttga
     1441 aatagtaagg atttctttca gctatatggt agttagacga taagagattt gttgttttga
     1501 atgcactcat ctccgggagt tctggattga gagcccagtc actcttctga gcaaataact
     1561 gataccagtt gttgagacct tctttcttgg ctaattcttc tatcttctcc ttaggtgtat
     1621 atttgggatg aaactgtttt aaatagtgct tgggtgcata caggtcctgt ccttcagcta
     1681 ccctccctac aaagtaaccg tatgggtcct ggaatctgaa tcttactgtg tagtcgtcta
     1741 tcttctccac agtaacctgc ttacccttca ttacaagcca cgatgggaac gtaggtgtga
     1801 gctctttatt tagaagtata tcctcatagt agaacataat gtcgtcagct gtaaatggtt
     1861 gtccgtcaga ccacttcaat ccctttctga tatagaaggt aaatgttttg ccattatcgg
     1921 agactttcca ggactttgct agattgggat gtatctttct atgggcacaa tcccagtaga
     1981 ctaaaggttc atgtgccatt ctccaccagc cagtcttatc gcccatgccg agatgagcct
     2041 ctcccctcaa tgttccccca tactgtccta tctcctcata aggtactacc actaatggat
     2101 cttcgggtag cctcttctca acagatggaa gtttaccctg ttttaccaat tctgctaacg
     2161 caggagcctc attgaatttt gtgattttcg tcccggtgag cttctggtaa tcagaaagat
     2221 tatattgatc tccaggcatc acctttgatt gagaaaagga tactggactg atgcttacaa
     2281 ttagactgaa aacaaccatt aaagaaatta acaaccttgc agaacttctc attttatccc
     2341 tcctatttgt tttatattac aaaacgctgt atacctattc ctgtaacaat tatatcaaga
     2401 atctcagtta tgtcaagagc cagaccttct tcctctgaat ctcaatttca tggtgagtat
     2461 tctatctgtc gagaagattt ttcctgctat aaatattaag atgacaaata ttcctgacat
     2521 atataaaatt ccatagaaaa caatatgata atttccgata agaagatttt gagatgcaag
     2581 gaatggatgg ctaaatggta ttgccaatat taagatcttt aatggtaaag atagggagtt
     2641 tatgtctgag aagatagaga tgaaataagg tattaagaca agaaacatca aaggtaagaa
     2701 tgcgctctgg gcactcttta gatcatctgt aagggcaccc aggatagtcg ctatagccag
     2761 ggcacacagt attgctaaaa ataaagaaat acccagaaat atataactgc tggaggtaaa
     2821 tcttaatccc aacttatcta ttactccttc aatctgagtg ttggatggga gactccctgt
     2881 aaatccaccc atataaaatc taaaccctat catatagatc ccggcagata acagtccaat
     2941 taagcctgaa gcaaacatct ttgccataac tatataattt ctgcttatgg ggactgtaag
     3001 cagagtctct aacgtcttgt cctgcttctc catagctata gcggagagga ccatctgaga
     3061 agagaatgtt attaccatca ttaaaataat tggaataaat atagactgaa aatagacaaa
     3121 attagcaaca tcagtaacag aaccttcagc catcctgtct ttaatcacaa taaaatttct
     3181 gctttttata gggtttttta agtcttctgg agtaatatct gagatcctat tccttataag
     3241 attatctgaa agataattgt ttactatacc aataagttgt ttaacaatct ctgtgtctga
     3301 catggtactc acagaaaagc tacgaatgaa agaatatacc tcaagttctt ctggttgaaa
     3361 ctttaaaaca gatggtccaa agccttccgg gatgattaat agcaagttta tattgttctt
     3421 tctggcaaaa tcaaccgccg attctctgtc tttaacatcc aggaggttga tcttgaagtt
     3481 ggcaagtgct aaattcctga ggatttcgcc tgaaaggtct gaatcgtcta aattgaggat
     3541 gtatatatcc ttagctcctc ctattttatt catctctgtt tttgccatat tccctataaa
     3601 ataaaaaagt attacggtga aggctaagga cagtataagt tgtgcactga ttaattcttt
     3661 tatctccttt ttaaaaagat tcataaactt cctcatctta ccaacctcct gaaaacctcc
     3721 tctagatttc ttacagaata tttctctttc aattcttcag ggctcccaat ctctaaaatt
     3781 tctccgctat caataatagc gaccctgtct gataagaatt ctatctccaa catattatgc
     3841 gaggataaca gcacagatat cccttctttc acaaatcccc ttatgatctc tctaatctct
     3901 aatgaattta ttacatccag cccagatgtg ggttcatcca gtatggctag ttcaggcata
     3961 gtcatcagag ctctggcaag tagaagtttt cttaccatcc ctttactata tgttcctatc
     4021 ttgtctttta atctgtctcc cagttctgca atctcttctg ccctcctgat gaatccttcc
     4081 acttctcttt cgtctcgggc atagaaatta gccataaacc tgagatattc tataccggtc
     4141 agatttttat atgcgccagc ttcttcaggt agataactta tattttctct caccctttct
     4201 ggatttttct ttacatcata tcccataaaa tttaccctgc catcatccgg ggagagcaat
     4261 gtggcaacaa ttctcagggc agtcgtcttt cctgccccgt taggacctat aagggcaaag
     4321 acctctccag tgctgacact aaaatctatc ccctttagag cagagacctt gccataagat
     4381 ttcttcagat tttccacatc aagaattaca tccataatga cctcctgttg aagattaatt
     4441 cagtccagcc tattatataa gtatctccaa atattttaca gtatatcata tcttgacttt
     4501 atagtatcta tatggtaaat tttaaactgt caactagatc ttttaggagg tagacataac
     4561 agtgtatgct attatagaga caggtggaaa acagtataaa gtaagccctg gggatatact
     4621 aacagtagag aaactggata ttgaagaagg ggaagaggta gtattcgata aggttttaat
     4681 gttgaaagct caggaaggag agagcgttat aggtactcct tacattgaga acataaaggt
     4741 aagaggtatt gtcattgaac agtttagagg caagaagatt atcgtgttta aatatagacc
     4801 caagaaacat tacagaagga agagaggaca tagacagtat ctctccagag taaagataga
     4861 agaggtgctt tatggctcat aaaaaaggtg caggcagtac atcaaacgga agagatagta
     4921 gtcctaaata tctgggggta aagcgttctg atggtcagtt tgtggtggca ggaaatatca
     4981 ttgtaagaca gagaggaacc aggatccatc ctggacagaa tgttgggatt ggtaaagatt
     5041 ttacc
//
LOCUS       DAOTKU010000061         5029 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_14681_length_5029_cov_3.003016, whole genome shotgun sequence.
ACCESSION   DAOTKU010000061 DAOTKU010000000
VERSION     DAOTKU010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5029
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14681_length_5029_cov_3.003016"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="P5310_02395"
     CDS             <1..278
                     /locus_tag="P5310_02395"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HRU32246.1"
                     /translation="TSAENAAREREILEKIQVLETKREEKSKGIDSKLLARYEAIRKN
                     RRGRGMSKVIDGKCSECGVELSLSIVEEMKHQEKILTCEHCGRILCT"
     gene            complement(332..2989)
                     /gene="ppdK"
                     /locus_tag="P5310_02400"
     CDS             complement(332..2989)
                     /gene="ppdK"
                     /locus_tag="P5310_02400"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HRU32247.1"
                     /translation="MKKWVYLFDEGDPNDRALLGGKGAGLAQMTQAGLPVPPGFTITT
                     EACNAYYEYNQQFPEGMWEQVLDALKEVERRTGKKFGDPSNPLLVSVRSGAKFSMPGM
                     MDTVLNLGLNDEVAKGLINLTQNERFVYDAYRRFIQLFGKIVLGIPGEIFDKAFEDYK
                     EKVGAKLDTDLTAEHLKEIVSIFKDIVKRETGSDFPQEPIEQLRLAIGAVFRSWNNKR
                     AIDYRNYNKIPHSLGTAVNIVTMVFGNMGFDSGTGVAFTRDPATGEKKFFGEFLFNAQ
                     GEDVVAGIRTPLKIEELKEKMPAIYDQLVEIAKKLEGYYRDMQDIEFTVERGKLWMLQ
                     TRSGKRTAQAAVKIAVDMAEEGIISKEEAILRVDPNQIDQLLHRQIDPSAKVTVIAKG
                     LNASPGAATGTVVFDADRAKELAKDGVKVILVRPETNPDDVGGMLVSEGILTARGGAT
                     SHAAVVARGIGKPCVVGAESIKIDLEKRLFNVNGYTVKEGDIITINGTTGEVIVGEAP
                     LIEPELTPELQKLLSWADSFKRLQNWANADYPRDAQKAKEYGAQGIGLCRTEHMFFEA
                     ERLPIVREMILARNSEERKSALDKLLPIQKEDFKGILKVMDGLPVIIRLLDPPLHEFL
                     PRHEELLAEVVELRVTGKDPKALAEKEELLRRVEEMQEVNPMLGLRGCRLGITRPEIN
                     EMQVRAIFKAACELKKEGYNPKPEIMIPLVGHVNELKLVRDQLESVAKKVMEEEGITV
                     DYKFGTMIEVPRAALTAGEIAEYAQFFSFGTNDLTQMTFGYSRDDAEGKFLFVYLEKG
                     ILPADPFQTLDIKGVGRLMEMAVKDGRSTRPELEIGICGEHGGDPDSITFAHEIGLNY
                     VSCSPFRVPIARLAAAHAALKEKQSFRDK"
     gene            3208..3675
                     /gene="rimP"
                     /locus_tag="P5310_02405"
     CDS             3208..3675
                     /gene="rimP"
                     /locus_tag="P5310_02405"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HRU32248.1"
                     /translation="METRENIISLIEKEVGKVIENMGYELIDVEYTKERGTWILRLFV
                     DRLDGGITLKECEGISHVVSLLLDELDPIPYSYNLEVFSPGLDRILKKPKDFARFIGH
                     KAQFKLKDPIKGSKNIKATIKDVVSSAVIVERDRDVFSIPFENILKARLEVEV"
     gene            3672..4760
                     /gene="nusA"
                     /locus_tag="P5310_02410"
     CDS             3672..4760
                     /gene="nusA"
                     /locus_tag="P5310_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HRU32249.1"
                     /translation="MTDNLLLMLKQIEKEKGISIESLFSFLEAALLSAYRKQYGTTQN
                     VSVIIDRNNGDIHIYAKKSVVEKVENNAIEIGLEDALKVNPEVKVGDVIDIEIIPSAN
                     FGRFAYQTAKQVITQKLEEAEKTILYEEFSKRIGEITRGTIYRIDKKGVYLDLGRVEG
                     VIPPYEQVDRENYKVGDKLVVYILKVIRKSQGPEITCSRSHPNLVKKLFELEIPEIQN
                     GIVVIKGIAREPGIRTKIAVASKDSKIDPAGTCIGAKGIRIQPIVRELKKEKIDVIVW
                     NEDPLIFVGNALNPASVIKVEQTGEKSARVLVAKDQLPLAIGKEGQNVRLAVKLTGWK
                     IDIKTEEERLEKTEENRTFVYSLQEEEK"
     gene            4777..4992
                     /locus_tag="P5310_02415"
     CDS             4777..4992
                     /locus_tag="P5310_02415"
                     /inference="COORDINATES: protein motif:HMM:NF016204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HRU32250.1"
                     /translation="MVKTPEGLIKLDPLKMFPGRGAYICGKIECISNFGKKNYLAYAL
                     KTRIEKDKLMDIKRELENLLQRGDVKR"
BASE COUNT         1597 a         1039 c          980 g         1413 t
ORIGIN      
        1 caaccagcgc agagaacgcc gcccgggaaa gggaaatcct ggagaagatt caggtattgg
       61 agaccaaaag agaggaaaaa tccaaaggga tagattccaa acttcttgca aggtatgaag
      121 cgataagaaa gaacagacgt ggaagaggca tgtcaaaggt cattgatgga aagtgttctg
      181 aatgcggagt cgaactttca ctatctatag ttgaagaaat gaaacaccag gaaaagatac
      241 ttacctgtga acattgtggc agaatactct gcacttaaaa tataaaaaac aaaagcgggg
      301 agagtctctc tccccgcttt ctcattattt cctacttatc tctaaatgac tgtttctctt
      361 taagggctgc atgtgcagct gctaaacgag cgattggaac tctaaatgga gaacagctta
      421 catagttgag ccctatctca tgggcaaatg ttatagaatc tggatctcca ccgtgctctc
      481 cacagattcc aatctccagt tctggtctgg tagaccttcc atctttcact gccatttcca
      541 taagtctacc cacacccttt atatccagag tctggaatgg atctgccgga agaataccct
      601 tttccaggta tacaaacagg aattttcctt cagcatcatc tctggaataa ccaaatgtca
      661 tctgagtaag atcatttgtt ccaaaactga agaactgagc atactcggca atttctccag
      721 ctgtaagcgc tgctctcggt acctctatca ttgttccaaa cttataatca actgtaatac
      781 cttcctcttc cataaccttc ttagcaactg actcaagctg gtctcttaca agcttaagct
      841 cgtttacatg ccctaccaat gggatcataa tttcaggctt tggattatat ccctctttct
      901 taagctcaca agcagcctta aatatagctc ttacctgcat ttcattaatc tctggtctgg
      961 tgataccgag tctgcatcct ctcagcccta acattgggtt aacctcttgc atttcttcta
     1021 cacgtctcaa taactcttcc ttctctgcca gggctttagg gtcttttcct gttaccctga
     1081 gttcaaccac ctctgccagt aactcttcat gtctgggtaa gaattcatga agaggtgggt
     1141 ccaagagacg gataataact ggtagcccat ccataacctt aaggataccc ttgaaatctt
     1201 ctttctgtat aggaagcagc ttatccagag cactcttcct ctcttctgaa tttctagcaa
     1261 ggatcatctc tcttactata ggcaatctct ctgcctcaaa gaacatatgc tcagttctgc
     1321 agagtccaat accttgcgcc ccatactctt ttgccttctg agcatctctc ggataatctg
     1381 cattagccca gttctgcaat ctcttgaagc tatcagccca agaaagaagc ttctgaagtt
     1441 caggagtgag ttccggctcg attagaggag cttctccaac gattacttca ccagtagtcc
     1501 cgtttatggt gattatatcc ccttccttta ccgtatagcc atttacatta aataatcttt
     1561 tctccaggtc aatctttata gactctgccc caaccacaca tggttttcca atacctcttg
     1621 ctaccactgc agcgtgactg gttgctccac cacgagcagt aagaatccct tcacttacca
     1681 gcatcccacc aacgtcatct ggatttgtct ccggtcttac aagtataacc tttactccat
     1741 cctttgccaa ttctttagca cgatcagcat caaagacgac cgtaccagta gctgctccag
     1801 gagaggcgtt caatccttta gcgataactg tcaccttagc agatggatca atctgtctgt
     1861 gtagtaactg atctatctgg ttaggatcaa ctctgagtat tgcctcttcc ttagagataa
     1921 ttccttcctc agccatatca acggcaatct ttactgctgc ttgggctgtt cgttttccag
     1981 accttgtctg aagcatccag agttttccac gttctacagt aaactctata tcctgcatat
     2041 ctctataata gccttccaat ttcttcgcta tctccaccag ctgatcatat atggcaggca
     2101 tcttttcttt taactcttct atcttcaatg gggttcttat acccgcaacg acatcttctc
     2161 cctgagcatt gaataagaac tctccaaaga atttcttctc tccagtggcg gggtctcttg
     2221 taaacgccac acctgtacca gaatcaaatc ccatatttcc aaataccatg gtaacaatat
     2281 ttactgctgt cccaagacta tgtggaatct tattataatt tctatagtct atggcacgct
     2341 tgttattcca ggatctgaat actgcaccta tagctaatct tagttgctct atcggctcct
     2401 gagggaaatc acttcctgtt tctctcttta ctatatcttt aaagatagaa acaatctcct
     2461 tcagatgctc agcagtgagg tctgtatcga gttttgctcc aaccttctcc ttataatctt
     2521 caaaggcttt atcgaagatc tctccaggga tacccagaac aatcttacca aaaagctgaa
     2581 tgaaacgtct ataagcatca tagacaaaac gttcattctg agtaagattt ataagtccct
     2641 ttgccacctc atcatttaat cctagattga gaacagtgtc catcatccca ggcatagaga
     2701 acttggctcc agaccttacc gacacaagca atggattgga tggatcccca aactttttcc
     2761 ctgtcctgcg ctctacttct ttaagagcat ctaatacctg ttcccacatc ccttctggga
     2821 actgttgatt atactcataa taggcgttac aagcctctgt ggtgatagta aatcctggag
     2881 gtactggaag ccctgcctgg gtcatttgag ctaacccggc gcctttacct cccaagagcg
     2941 ccctatcgtt aggatcgccc tcatcaaaaa gataaaccca tttcttcatc tttcacactc
     3001 cttctacaaa aaaatttacg agttaaatgt ctgattcaaa cctaatctat tatacaaaaa
     3061 agataaaaaa gaatcaaccc tccatagagt acagataaac taataatttg ctgcatttgc
     3121 attttatatc agattatgtt aaaatattca aaaatagata aatagagaaa ggagacgaaa
     3181 agtgggcttt gtcccacttt ttataatatg gagacacgag aaaacataat ttctctgata
     3241 gagaaagaag taggaaaagt tatagagaat atgggatatg aattaataga tgtggaatat
     3301 actaaggaaa gaggaacatg gatccttcgc ctctttgtag acagactgga tggtggtata
     3361 accctcaaag agtgtgaagg tataagccat gtagttagcc tattacttga tgagcttgac
     3421 cctatccctt attcatataa tctggaagtc ttctctcctg gattggatag aatattaaag
     3481 aagccaaaag actttgcaag atttataggg cataaagctc agtttaagct taaggaccct
     3541 attaaaggtt ccaaaaatat aaaagctacc attaaagatg ttgtaagcag tgctgtgata
     3601 gtggaaaggg atagagacgt attttctatc ccctttgaga atatcttgaa agcgaggcta
     3661 gaggtagaag tatgaccgat aatctcttgc taatgttaaa acagatagag aaagaaaagg
     3721 gtatatctat agagtctcta ttctcttttc tggaagctgc actactttct gcctatagaa
     3781 aacagtatgg aactactcag aatgtatctg taatcattga tagaaataat ggagatatac
     3841 atatatatgc taaaaagagt gtggtagaaa aagtagaaaa taatgctata gagataggtc
     3901 tcgaagatgc attaaaggtc aacccggaag taaaagtggg agatgtgata gacatagaaa
     3961 taatcccatc cgctaacttt ggaaggtttg cctatcaaac tgcaaaacag gtaatcactc
     4021 agaagttaga agaggcagaa aagactatac tctacgagga attctcaaaa agaatagggg
     4081 agattacaag gggtactatc tatagaatag ataagaaagg ggtatatctg gatcttggca
     4141 gggtagaagg ggtaatacca ccatatgaac aggtagatag agaaaattat aaggttggag
     4201 ataagttagt tgtatatata cttaaagtaa ttagaaaatc acagggacca gagataacat
     4261 gttctagaag ccatccaaac ctcgtcaaaa aactcttcga gctagaaata cccgaaattc
     4321 aaaacggaat agtggttatt aaaggaatag ccagggaacc gggtataagg accaagatag
     4381 cggtagcatc aaaagatagt aaaatagacc ccgctgggac atgtataggt gctaagggaa
     4441 tcaggattca gcctatagta agagaattaa aaaaagaaaa gatagatgta atagtatgga
     4501 atgaagaccc attaatattt gtaggaaatg cattaaatcc agcatcggta ataaaggtgg
     4561 aacagactgg ggaaaagagt gcaagagtat tggtggctaa agatcagctc ccattggcta
     4621 tcggaaaaga agggcagaat gtaagattgg ctgtgaaact tactggatgg aagatagata
     4681 taaagacaga agaggagcgc ttagaaaaga ctgaagaaaa tagaacgttc gtgtatagtt
     4741 tgcaggaaga agaaaaataa agaggagctt ataaggatag taaaaacccc tgagggtcta
     4801 ataaaattag accctctaaa gatgtttcct ggcaggggag catatatatg tggtaaaata
     4861 gagtgtataa gtaattttgg aaagaagaat tatctggcat acgctcttaa aaccagaata
     4921 gagaaggata aattaatgga tataaagaga gaattagaga atcttctgca aagaggagat
     4981 gttaaacggt gagagtttat gaattggcta aaaaattggg ggttagtac
//
LOCUS       DAOTKU010000062         5016 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_14719_length_5016_cov_3.407982, whole genome shotgun sequence.
ACCESSION   DAOTKU010000062 DAOTKU010000000
VERSION     DAOTKU010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5016
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14719_length_5016_cov_3.407982"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /locus_tag="P5310_02420"
     CDS             <1..619
                     /locus_tag="P5310_02420"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014957573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HRU32251.1"
                     /translation="VESLGLKYVVITSVTRDDLPRGGAEEFRDTVIEIKRLNRNVNVE
                     VLTPDFRGDKEALFEALSAGPDVLNHNIETVPSLYPTVRPMADFERSLNILYWAKEYN
                     NDIITKSGIMVGLGETDEELYNTFKMLADVGCDILTIGQYLQPTREHLEVREYVTPEK
                     FQELERLAYKSGLKYVVSGVFVRSSYFAHKGYEKILEVKNRCTDS"
     gene            604..1311
                     /locus_tag="P5310_02425"
     CDS             604..1311
                     /locus_tag="P5310_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32252.1"
                     /translation="MHRFLSLVLAVIISISVFSFVYAQGTKPLVQVSFKVIITDPLKY
                     DLGKPIPEVDIEKLNLTSEQVDKLTKLREKLQESEVKYQRDFADIWNKELKPATQKED
                     QEATKKAREKLLNVLKELQKARINYYFEMQKVLTEEQLKHFPILFTREQVDKFEKIRE
                     SYDENRLNLDIARLIEAYKVQELRKGNASTKDINTRVALIVAIDNKKKDDLINYRVSL
                     REVLTKEQREILFGEKK"
     gene            1314..2387
                     /locus_tag="P5310_02430"
     CDS             1314..2387
                     /locus_tag="P5310_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003248696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HRU32253.1"
                     /translation="MDKIDLLLKDLTETPGISSFEDRIKERMRYYLSPLGEITCDKLG
                     SILCEKRGESSYPRIMIAGHMDEIGFIVKYVTDEGFLKFAPIGGWWSQVLPSQRVIVY
                     SKNGEFPGVVGSKPPHLMLPDEREKPVKLDDLFIDVGAKNREEAENDFGIKPGSPVVP
                     WSPYQVMGKDGKFLMGKAWDDRIGCAVIIRVLEDLKGERHPNTVIGAGTVQEEVGLRG
                     AETSVKVAKPDIAFVVESGIAGDTPNVKKDESDVKLGKGPTIGMMDSSMLPNRKLVEF
                     VEETARELGIPYQYEFMLRGGEDGGRIHLYNSGVPTVTINVPARYIHSHTGVIHRDDF
                     ENTVKLLENLIKRLNAEKVLRFI"
     gene            2384..3766
                     /gene="gltX"
                     /locus_tag="P5310_02435"
     CDS             2384..3766
                     /gene="gltX"
                     /locus_tag="P5310_02435"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HRU32254.1"
                     /translation="MSVRVRFAPSPTGYLHIGTARTALFNWLFARREKGVFILRIEDT
                     DLERSKKEYEDAIISDLEWLGIDWDEFYRQSERLWLYKEIADNLLSDGKAYECFCTKE
                     ELEKRRGQGYDRRCLNLSEAEKDTLRKERRPAMRLFVAKEGETRFYDYLHGEISFQNN
                     LIDDFVILKSDGTPTYNFSVVVDDIYMNITHVIRGEDHISNTPKQIQIYRALNRDLPV
                     FVHIPMILGQDKAKLSKRHGATSIGEYREMGYPSEAIVNFLALMGASYDDRQVLSREE
                     LVSLFSLDNLSKNPAIFDIKKLEFLSQEHIRMKDPKELASLIKDTLGKEGLGHWAEDD
                     LHFHRVVALLQSRMKTLKDFAVMADFFFTEEFARDVEVPPEERERIIAILPDLIDRLL
                     KIEAWNSNELEEKIRGFITEKELDSRWFLQMLRILISGKKVTPGIFETIEVLGKERTI
                     NRLKIWGGLV"
     gene            complement(3926..4207)
                     /locus_tag="P5310_02440"
     CDS             complement(3926..4207)
                     /locus_tag="P5310_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010869934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HRU32255.1"
                     /translation="MNIEEIREKILPILKKYKVKRAGIFGSMARNEETEKSDIDILVE
                     LPDEASLLDLVGLKMELEEVLRKKVDVLTYDSIYPPLREPILSEEVAIL"
     gene            4473..4940
                     /locus_tag="P5310_02445"
     CDS             4473..4940
                     /locus_tag="P5310_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012003551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HRU32256.1"
                     /translation="MGIEEILSKYEPKMENILLILHELQNARPGQFLSREDFEGVAKY
                     LNLPLSKVYETATFYGLFSTKPRGRYIIRVCVSTPCYVEGSEEVMKTIEGLLRIKPGE
                     TTEDGLFTLEEVSCLGICGVAPAMMINEEAFGNLTSERVAEIINSFRERGEEK"
BASE COUNT         1683 a          850 c         1172 g         1311 t
ORIGIN      
        1 ggtggaaagc ctaggattaa agtatgttgt tattacatcg gtaacaagag acgatctgcc
       61 aagaggaggt gcagaagaat ttagagacac agtaatagag ataaagaggt taaacagaaa
      121 cgtgaatgta gaggtcctga caccagattt tagaggggac aaagaggcac tatttgaagc
      181 attaagtgca ggtccagacg tcctaaatca caatatagag acagtccctt ctctataccc
      241 cacagtaaga cctatggctg actttgagag gtcacttaat atactctact gggctaaaga
      301 gtataacaac gatataatta ccaagtcagg tattatggtg gggctaggag aaacagatga
      361 agaactgtat aacacattta aaatgctggc agacgtaggc tgtgatatcc tcaccatagg
      421 acagtatctt cagccaacca gagagcatct ggaggtaaga gagtatgtca cgcctgagaa
      481 gttccaggaa cttgaaagat tggcttataa atcaggatta aaatatgtag tatccggagt
      541 ttttgtgaga agctcctatt ttgctcacaa gggctacgaa aaaatcttgg aggtgaaaaa
      601 tagatgcaca gattcctaag tttagttctt gctgtaatta tcagtatttc agtgtttagt
      661 tttgtctatg ctcaggggac aaagccttta gtacaggtaa gtttcaaggt aataataaca
      721 gacccgctta aatatgacct gggtaagccc atcccagagg tagatataga gaagctcaat
      781 cttacctctg aacaggtaga taaattgact aagttgagag aaaaactgca ggagagtgag
      841 gtaaagtatc agagagactt cgcagatatc tggaacaaag aattaaaacc ggcaacacag
      901 aaagaagacc aggaagctac aaagaaagcc agagaaaaac tgttgaatgt gcttaaggag
      961 cttcagaagg cacggataaa ttactatttt gagatgcaga aggtattaac agaggaacag
     1021 ctgaaacatt tcccaatact ttttaccaga gaacaggtgg ataaatttga aaagatcaga
     1081 gaaagttatg atgagaacag actaaatctg gatatagcca gattgataga ggcatacaag
     1141 gtgcaggaac tcaggaaagg gaacgcttct accaaagata taaacacaag ggtggcactt
     1201 atagtagcta tagataataa gaagaaggat gatttaataa attatagggt ctcattgaga
     1261 gaggtcctca ccaaagaaca gagagaaatc ctgtttgggg agaagaaata gggatggaca
     1321 agatagacct cttattgaaa gacctcacag aaactccagg tataagcagc tttgaggata
     1381 ggataaaaga gaggatgaga tattatctct ctcctctggg agagattacc tgcgacaaac
     1441 tgggaagcat cctctgcgaa aaaagagggg agagtagtta tcccagaata atgatagctg
     1501 gtcatatgga tgagataggt ttcatagtaa agtatgttac tgatgaaggc tttctcaagt
     1561 ttgccccgat aggtggctgg tggagtcagg tattaccatc acagagggta atagtatact
     1621 caaagaatgg ggaatttcca ggtgtggtag gttctaaacc gccacatctg atgctgcccg
     1681 atgagagaga aaagccggta aaacttgatg atctattcat agatgttgga gcaaaaaaca
     1741 gagaagaggc agagaatgat tttggtataa aaccaggttc gccggtggta ccctggtcac
     1801 cctatcaggt gatgggcaaa gacggaaaat tcctgatggg aaaagcctgg gatgacagaa
     1861 ttggctgcgc agtaattatc agagtactgg aagacctgaa aggagaaaga catcccaaca
     1921 ctgttattgg agcaggaaca gttcaggaag aggtcggact cagaggggct gagacaagtg
     1981 taaaggtggc caaaccagat attgcatttg tagtagaatc aggcatagca ggggataccc
     2041 caaatgtaaa aaaagatgag tcggatgtaa agctgggaaa gggacccact atcggaatga
     2101 tggacagcag tatgttacca aatagaaagc tggtagagtt tgtggaggaa acggcgagag
     2161 aactggggat tccataccag tacgagttta tgctcagagg tggagaagat gggggaagaa
     2221 tccatctata taattctggt gtacccacag taacaattaa tgtcccggca agatatatac
     2281 atagtcatac tggagtaatc cacagggatg actttgagaa tactgtaaaa ttactggaga
     2341 atctgattaa gagactgaat gcagaaaagg tgctcagatt catatgagtg taagggtaag
     2401 gtttgctccc agtcctaccg gatatttaca cataggaaca gccagaacag ctctctttaa
     2461 ctggttattt gctagaagag aaaagggggt ttttatcctc aggatagagg atactgatct
     2521 ggaaaggtca aagaaggagt atgaagatgc tatcatctcg gaccttgaat ggttagggat
     2581 agattgggat gaattttaca gacagagtga gaggctctgg ctttataaag agatagcgga
     2641 taacctctta tctgatggta aggcttacga gtgtttctgc acgaaagagg agctggagaa
     2701 gaggagagga caggggtatg acagacgctg tctgaatcta tccgaagcag agaaagacac
     2761 cttaagaaaa gagagaagac cagcgatgag gttattcgtc gccaaagagg gtgagacacg
     2821 attttatgac tatctccatg gagagatcag cttccagaat aatctcatag acgatttcgt
     2881 gatattaaag tcagatggta cacccacata taatttcagt gtggtggtgg atgacatata
     2941 tatgaacata acacacgtta taagaggaga ggatcacata tccaatactc cgaagcagat
     3001 acagatatac agagctctta acagagacct ccctgttttt gttcatatac ctatgatact
     3061 tggacaagat aaagccaagc taagtaaaag acatggagca acatcgatag gagaatatcg
     3121 cgaaatgggt tatccttcag aggcgatagt aaattttctg gcgcttatgg gggcttccta
     3181 tgatgacagg caggtccttt cccgggaaga acttgtaagt ctcttttcgc tggataatct
     3241 gagtaaaaat ccagcaatat ttgacataaa gaagcttgaa tttttatccc aggaacatat
     3301 aaggatgaaa gatccgaaag aactcgcctc tctgataaaa gataccctgg gaaaagaagg
     3361 tctgggacac tgggcagagg atgatcttca ctttcatcgg gtagtggcat tgcttcaatc
     3421 ccgaatgaaa accctgaagg actttgctgt tatggcagac ttcttcttta ccgaagagtt
     3481 tgctagagat gtggaggtcc caccagaaga aagagaaagg ataattgcca tccttccaga
     3541 tctgatagat agattgctca agatagaggc gtggaattca aacgagctgg aagaaaaaat
     3601 aagaggtttt ataaccgaaa aagaattgga ctcaagatgg tttttgcaga tgttgaggat
     3661 tttaatctct ggcaaaaaag ttactccggg gatttttgag actatagaag ttttaggaaa
     3721 agagaggaca ataaatagat taaaaatctg gggtggactg gtctagtccg cccttccttt
     3781 aagaatcata gttctatgag gggagaatac ttccctctcc caacctcacc ttaatcctcc
     3841 cccatctcat taaatctttt tacatattca agcccctctt ctagcgtgtt tataaaaaat
     3901 gttatattta ccagtggatc ttttctcata atattgctac ttcttcgctt aatatgggtt
     3961 ctctaagagg cggatatata gagtcataag ttaatacatc aacctttttc ctaaggactt
     4021 cttctaattc cattttaagt ccgacaaggt caagaagact cgcttcatca ggcaattcta
     4081 caagaatatc tatgtcactc ttctcagttt cctcattcct tgccatagag ccaaagatcc
     4141 cagctctttt tactttatac ttttttaata taggtaatat tttctcccta atctcttcaa
     4201 tattcatcta tcattaaatc tttcttttta tttttagcca tgttttatcc ctgtatatta
     4261 ttgtatcatg ataagattcc cactgaaaat ataaagacag ccatttcaga tctttgtatc
     4321 gtttttgtat cctgtagggg caattcatga attgccccta caatttaccc atgacaatat
     4381 cacagaacgt taacagttcg cttgactata gtcggctaat ctattaatat aaactaggag
     4441 caaaattact taaataagta aggaggaaga gaatgggtat agaagaaatc ctgtctaaat
     4501 atgaaccaaa aatggagaat atactcctta tacttcacga attgcaaaac gcaagaccag
     4561 gtcagtttct ctccagagaa gattttgagg gggtagcaaa atacctaaat ttacctctgt
     4621 ctaaagtcta tgaaactgct actttttatg gacttttcag taccaaaccc aggggcagat
     4681 atataataag ggtatgtgta tccaccccct gttatgtaga ggggtcggaa gaggtgatga
     4741 aaacgataga ggggttactc cggattaagc ctggagagac cactgaggat ggtctcttta
     4801 cccttgaaga ggtaagctgc ctggggatct gtggagttgc tccagctatg atgataaatg
     4861 aggaggcttt tggaaatctc acatctgaaa gggtagcaga aattataaat tcatttagag
     4921 aaagaggaga agaaaaatga tagtaagaac tcacattctt ataggaatag atgcttcttc
     4981 tattacttta ttttgtttag ctaaaggaaa tagtca
//
LOCUS       DAOTKU010000063         4911 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_15082_length_4911_cov_2.816104, whole genome shotgun sequence.
ACCESSION   DAOTKU010000063 DAOTKU010000000
VERSION     DAOTKU010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4911
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15082_length_4911_cov_2.816104"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..150
                     /gene="ssrA"
                     /locus_tag="P5310_02450"
     tmRNA           <1..150
                     /gene="ssrA"
                     /locus_tag="P5310_02450"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            158..1486
                     /locus_tag="P5310_02455"
     CDS             158..1486
                     /locus_tag="P5310_02455"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRU32257.1"
                     /translation="MEKKAKLYLHVIFIFIFLFTVSPVYGEETVGNELEQNPNEDSTS
                     YLNNINFASSEDGDIFTIDITGDFKFDYSYLQNPYRFFIDLYPVKSNLNETELPVNID
                     GIEKIRIAKQDEDKVRVVFDLKGPLRYKDIKRGDSGLIITFPPRIKEISLKDEEGGIT
                     LTISSTYTGYLNPDVFYLTNPDRIVIDIPNTTLMYDNYIMNNNTLSEVKISQYSIYPP
                     SVRIVVKDEYKKGYTIEGSKEPGTIRLYINRPLTISSSKPVIILDAGHGGNDPGAIGP
                     SSLKEKDVTLDIVLYMKGLLESSGYTVILTRDGDYNVSQNSKDTRDELNTRVIFINNN
                     KSAILVSVHCNSANSPVPGGIEVYYYRDEDKKLAEFVCNSLVETTEKYNRGVKKADFF
                     LLKNTTVPAILVEALFISNPVEEKLLMDPLFRRKIAGAIVTGISRYLENR"
     gene            1486..2310
                     /gene="murI"
                     /locus_tag="P5310_02460"
     CDS             1486..2310
                     /gene="murI"
                     /locus_tag="P5310_02460"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HRU32258.1"
                     /translation="MKNAIGIFDSGLGGLTVLKELKQMLPDEDIIYVADTIHIPYGNK
                     SVQEIVCYMRQIVSFLEKMRVKAVIVACNTSSALALKYIENIISTPIIGLIEPAAEKL
                     SRKESIRKVGVLATRATVSSLAYTLAIRRYNPQIQVIERSAPLLVSLIENRAKKDMLF
                     KALSVYLGPLIEAQIDTLILGCSHYPLYLKEISSILLEKGSDIEIVDPARPAVEKLAL
                     ILRDTGIIGGGRGDIEFYTTGDAYDFKEKASIFWNGPVKVRHINLREVGEIKLWQR"
     gene            2298..2999
                     /gene="rpe"
                     /locus_tag="P5310_02465"
     CDS             2298..2999
                     /gene="rpe"
                     /locus_tag="P5310_02465"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003148320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HRU32259.1"
                     /translation="MAEIVPSILSADIIELKSLLDEFKTSNIEWLHIDIMDGHFVPNL
                     SFGPQIVESIKSSYRFLLDVHLMVTPPEDFIEPFRKAGSDLITVHIEATPHIHRTISY
                     IKSLNCRAGIAINPGTPILLLKEIIEEVDLVLLMSVNPGFGGQVFIRNAIRRLNELKE
                     LCASLGVSPLIEVDGGVNGENIENLIDCGVDMLVIGSAITRSKDRKSRLLELDNMMKR
                     RGQKIEEYNPNLKGR"
     gene            3005..3127
                     /locus_tag="P5310_02470"
     CDS             3005..3127
                     /locus_tag="P5310_02470"
                     /inference="COORDINATES: protein motif:HMM:NF024170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRU32260.1"
                     /translation="MGKKAPARCPKCGSTKILPKKGDPSKWECKSCGATFSANV"
     gene            complement(3168..3839)
                     /locus_tag="P5310_02475"
     CDS             complement(3168..3839)
                     /locus_tag="P5310_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020265618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRU32261.1"
                     /translation="MALISERDRNYLREFFERELVNEVKILLFTQRSGLFVPGVECEY
                     CRETEGLLKELSDISDKIELEIYDFITDEEMVKKYSIKRIPAIAILGDKDYGIRFYGI
                     PSGYEFSSLIEDIVDVSKRTTRLSKELKERLAKIDKETNIQVFVTPTCPYCPRMVRTA
                     HQFAMENDFITAEGIEVIEFPQLGNYYNVEGVPKTVINDKEEIIGAVPETAFLESIER
                     AIKSS"
     gene            complement(3855..>4911)
                     /locus_tag="P5310_02480"
     CDS             complement(3855..>4911)
                     /locus_tag="P5310_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HRU32262.1"
                     /translation="IDPNTGEIIVRTNEAIDDEKAKKIEAAGIERVRVFSVFTCKSKK
                     GVCAKCYGINLATNKLVDVGEAVGVTAAQSIGEPGTQLTLRTFHTGGVAVEDITQGLP
                     RVEELFEARKPKGQAIMSEVEGIVKVDDSKGVTYISIINDNVEKARYQIPFGSRIRVQ
                     DGDSVVPGTPLTEGPLNPHEILRIEGVKDVERYLVDEVKKVYISQGVELHDKHIELIV
                     RRMLQKIKVDDPGDTELIIGNLVDINEFEELQEMAIKNGLKPPRGKRILLGITRASLA
                     TDSFLAAASFQETTRVLTEAAVKGKIDTLSGLKENVIIGNLIPAGTGFPGYKNLSVEK
                     VEEGEKEEAAMEYFQVM"
BASE COUNT         1535 a          984 c          928 g         1464 t
ORIGIN      
        1 cacaggtgga agggtgattt aaagtgagct tacctctata gccctgcttc tggggcacta
       61 tagggggaca tataaaacag aagctaatcc tgtaggagcc ttttggtttc tcatctccgg
      121 acgcgggttc gattcccgcc gcctccacca tttatatatg gaaaagaagg caaaactata
      181 tctccatgtt atttttatct ttatctttct atttacagtt tctcccgtat atggggaaga
      241 aactgtagga aatgagcttg agcaaaaccc aaatgaagat agcacttcat atttaaataa
      301 tataaacttt gccagttctg aagatggtga tatatttact atcgatatca ccggagattt
      361 taagtttgat tattcttact tacagaatcc ttacagattc tttatagacc tttatcctgt
      421 taaaagtaac ctcaacgaaa cagaattacc agtaaatatc gatggtatag aaaaaataag
      481 gatagcaaaa caggatgaag ataaggtaag ggttgtcttt gaccttaaag gtcctctgag
      541 atacaaggat ataaagagag gagattctgg tttaataata acctttcctc cccgtataaa
      601 agagatatct ctaaaagatg aggaaggcgg tataactctt accatatctt caacttatac
      661 aggctacctg aatcctgatg tattctatct aaccaatcct gacagaatag tgatagatat
      721 cccgaataca actcttatgt acgacaacta cattatgaat aacaacactt tatcagaggt
      781 aaaaataagt caatacagta tatatccacc ttcagtcagg atagtcgtaa aagacgagta
      841 caaaaaaggt tacactatag aaggctcaaa agagccgggg acaattaggc tctatatcaa
      901 tcgaccactg acaatctcaa gctctaaacc tgttatcata ttagatgctg gtcacggagg
      961 taatgatccg ggggctatag gtccaagcag tttaaaagaa aaggatgtta cccttgatat
     1021 agtattgtat atgaagggat tactggaatc tagtggctat acagttattt taaccaggga
     1081 tggcgattat aatgtatcac agaattctaa agatacaaga gatgagctga atacaagagt
     1141 tatttttata aacaacaata aatctgccat actggtaagc gtacactgta attcagcaaa
     1201 ttcgccagtc cccggtggga tagaggtata ctactataga gatgaggata agaagcttgc
     1261 agagtttgta tgtaactcac tggttgaaac tacagagaaa tataatagag gggtgaagaa
     1321 ggcagacttc tttctgttaa aaaacacgac tgtcccagct attctggtgg aggctctatt
     1381 tatatcaaat cctgtggaag aaaaattact catggaccct ctattcagaa ggaagatagc
     1441 cggtgctata gtaactggaa tatcaagata tctggagaat agataatgaa aaatgcgata
     1501 gggatattcg attcaggatt gggaggattg acagtattaa aagagctcaa acagatgctt
     1561 cccgatgagg atataattta cgtggcagat accatacata tcccatatgg gaataaatct
     1621 gtacaggaga ttgtctgcta tatgaggcag atagtatcat tcctggagaa gatgagggta
     1681 aaagctgtaa ttgtagcctg taatacaagt tctgctttgg ctttaaaata cattgaaaat
     1741 attatatcta cccctataat tggacttata gaacctgcag cagaaaaact ctccagaaaa
     1801 gagagtatta gaaaagtagg agtcctagct accagggcaa ctgtttcttc tctagcgtat
     1861 actctggcta tcagaaggta taaccctcag attcaggtaa ttgaaagatc tgccccactt
     1921 ctggtctcat tgatagaaaa cagggcaaaa aaagatatgc tgttcaaagc actatctgta
     1981 tatcttggac cactcataga agctcagata gataccctca tattgggatg cagtcattat
     2041 cccttgtatt tgaaagagat tagtagtatt ctattagaga aaggtagtga tatagagatc
     2101 gtggaccctg caagacctgc agtagaaaag ttagctttga tattgagaga tactgggatt
     2161 atagggggag gaagagggga tatagagttc tataccaccg gagacgctta tgattttaaa
     2221 gaaaaggcaa gtattttctg gaatggtcct gttaaggtga gacatataaa cctgagagag
     2281 gtaggggaga ttaagttatg gcagagatag tgccatctat cctttccgct gatataattg
     2341 aacttaagag tctcttagac gaattcaaga cgtccaatat agaatggtta catatagata
     2401 taatggatgg gcattttgtt cccaatctaa gtttcggtcc acagatagta gaatccataa
     2461 aaagtagtta caggtttcta cttgatgtac atcttatggt aacccctcca gaggacttta
     2521 tagagccatt caggaaagca ggatcagacc ttataactgt tcatattgag gcaacaccgc
     2581 atatacacag aacaatatca tatataaagt cactaaactg tagagctgga atagctataa
     2641 atcctggtac ccccattttg cttcttaaag agattataga agaggtcgac ctcgttttac
     2701 ttatgagtgt aaatccaggt tttggtggtc aggtctttat aagaaatgca atcagaaggc
     2761 taaatgagct gaaagaactt tgtgcgtcac tgggcgtctc acctcttata gaggtagacg
     2821 gaggtgttaa tggtgaaaac atagaaaacc ttattgattg tggtgtagat atgctggtca
     2881 taggttcagc tataaccagg tctaaagata ggaaatccag attgcttgaa ttagataata
     2941 tgatgaagag aagaggtcaa aagatagagg agtataatcc taatttaaaa ggcaggtgag
     3001 agttatgggt aaaaaagctc cggcaagatg tccgaaatgc ggtagtacaa agattctccc
     3061 caagaaggga gacccttcaa aatgggagtg taagagctgc ggtgcgacat tctctgccaa
     3121 tgtatagaaa agaacagggg ctaccagttt ctggtagccc ctcaacttta actactcttt
     3181 attgctcttt ctatactttc taaaaaagcc gtttccggta ctgcccctat tatctcttct
     3241 ttatcattta taacagtctt agggacaccc tccacattat agtagtttcc aagctgagga
     3301 aattctatca cctctatacc ctcggcggtt ataaaatcat tttccatagc gaactgatgg
     3361 gcggtacgca ccatcctggg acagtaggga caggtcggtg taacaaaaac ctggatatta
     3421 gtttctttat ctatctttgc cagacgttct tttaattcct tagaaagcct ggtggttctc
     3481 ttactcacat ctactatgtc ctctatcaga gaagaaaact cataaccaga gggaatgccg
     3541 taaaatctta tgccataatc tttatctccg agaatagcaa tggcaggaat cctctttata
     3601 ctgtacttct ttaccatctc ctcatcggta ataaaatcgt aaatttccag ttcaatctta
     3661 tctgatatat ctgaaagctc tttcagcaaa ccctctgttt ctctgcagta ctcacactca
     3721 actcctggga cgaaaagtcc tgatctctgt gtaaaaagaa gtatcttcac ttcgtttacc
     3781 agttctcttt caaaaaactc tctcaaataa ttcctatctc tttcacttat aagtgccata
     3841 ttatcctcct gcaattacat cacctgaaaa tattccattg cagcctcttc tttttcccct
     3901 tcttccactt tctccacact cagattttta tacccgggga aacctgtgcc tgcaggaatc
     3961 aaattcccta taatgacgtt ttcttttaat ccagaaagtg tatctatttt accctttacc
     4021 gccgcctcag ttaatactct ggtagtctcc tggaaagatg ctgctgccaa aaaactatca
     4081 gtagcaagag aagctcttgt aatcccaagt aagattctct tgccccttgg tggtttcaga
     4141 ccgttcttta ttgccatttc ctgaagttct tcaaactcat ttatatccac cagattacct
     4201 attataagct ccgtatcacc gggatcatca acttttatct tctgcagcat tctccttact
     4261 ataagttcta tatgcttatc gtgcagttca actccttgag atatataaac ctttttaacc
     4321 tcatctacca ggtacctttc tacatctttt actccctcaa ttctcagtat ttcatgaggg
     4381 ttcaaaggac cttctgtaag tggtgttcct ggaacaacgc tatctccatc ctgaactctt
     4441 atcctggacc caaatggtat ctgatatcta gctttttcca cattatcatt tataatagaa
     4501 atataagtaa ctcctttact atcatctact ttgacaatac cttcaacttc actcataata
     4561 gcctgcccct taggctttct ggcttcaaat aactcttcta ccctgggaag accctgagtt
     4621 atatcttcca cggcaactcc accagtatga aaagtcctca acgttaattg ggttccaggt
     4681 tcacctatgg attgcgctgc agtaacccct acagcctctc caacgtctac tagcttattg
     4741 gtagcaaggt taataccata acacttggca catacacctt tcttagattt acaggtaaaa
     4801 acagagaata ctctcaccct ttctataccg gctgcctcta tcttcttagc cttctcatca
     4861 tcaatagctt cgttagttcg cacaataatc tctccagtgt tagggtctat t
//
LOCUS       DAOTKU010000064         4902 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_15105_length_4902_cov_3.251083, whole genome shotgun sequence.
ACCESSION   DAOTKU010000064 DAOTKU010000000
VERSION     DAOTKU010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4902
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15105_length_4902_cov_3.251083"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            304..876
                     /locus_tag="P5310_02485"
     CDS             304..876
                     /locus_tag="P5310_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015732531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2202 domain-containing protein"
                     /protein_id="HRU32263.1"
                     /translation="MKRIAGTIGILLILTVFLSFTDGGYTGAQQVSSLLTEEEVDSLL
                     LMREEEKLARDVYTVLYQKWGLRIFSNIATSEQRHMDSVKVLLDRYGIQDPVKDNQTG
                     KFTSPELAKLYDELVERGTKSLLDALIVGANIEELDIKDLIESSSKTDKADIKMVYDN
                     LKRGSENHLEAFIRQITLRGGSYTSKYITE"
     gene            complement(931..1269)
                     /locus_tag="P5310_02490"
     CDS             complement(931..1269)
                     /locus_tag="P5310_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32264.1"
                     /translation="MVINYIFGSYQISPVSADIIGGSTGPFEAKMIAEGTVADTNVAN
                     ALNAIALATSNIHLNAYTGERYQFLMNFSHGYGCTGDGDTLIGEYELIHYKQGTWIKI
                     DEGAVSLYRD"
     gene            complement(1273..1470)
                     /locus_tag="P5310_02495"
     CDS             complement(1273..1470)
                     /locus_tag="P5310_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32265.1"
                     /translation="MSDDEEEIFLLIVTGFFLLGAIGGCSTPNFSGDWSGTLTSSLNP
                     AVSGTVTITDLIQGVANNFVG"
     gene            complement(1474..2319)
                     /locus_tag="P5310_02500"
     CDS             complement(1474..2319)
                     /locus_tag="P5310_02500"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRU32266.1"
                     /translation="MHNSKIKTFTDGVLIVLAMIWTLLPLWWTFQTSISPEVDLTRVP
                     PRWFPSSINLDYYHSIFFEKIGTTTTFRASLFNSSLISITVALISVFLGSLAAYAIVR
                     LNVLSGQWVIFTLLFAQMIPPVILITSLYVIASRVHLLDTKFLLILVYSALQLPMAIW
                     ILRGYFQTIPREIEDSALIDGCSPSQVLFKIVLPLSGPALFTTGIFTFISSWGEFLMA
                     LVFTSSLSSKTMPVAIAEFMGRFTVDYGLMCAAGMIASVPPILIALIFQRFIIQGLTS
                     GAVKG"
     gene            complement(2319..3191)
                     /locus_tag="P5310_02505"
     CDS             complement(2319..3191)
                     /locus_tag="P5310_02505"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRU32267.1"
                     /translation="MSRKTLGYLLILPSAVIIIVVLVYPILYSGYLSFFETFVGPGTL
                     VLTPVGLKNYIETIKNPQWWWAFGRTAYFVLYDILVGMSLGLGIALLLNKEFRLRGIA
                     RALVLFPYVLPSIVKALMWKWIYNSDYGFLNGLLFQLGLIKDYIPWLSSPTLAIHMVI
                     LANIWEGTPFAIIMYLAALQTIPKELYEASEVDGATPWQMFMKITFRLIKPMTFILIV
                     IKTVATFKIFDLVYALTAGGPGGSTQVVGYLMYSTVFDSLQFGKGAAMSYILLAIILV
                     LVFIYSRLFQMEEA"
     gene            complement(3238..4503)
                     /locus_tag="P5310_02510"
     CDS             complement(3238..4503)
                     /locus_tag="P5310_02510"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRU32268.1"
                     /translation="MKKILVSVLVAILSFAIFSFGFGEEIELNTISGPDVDFLLKRVQ
                     QFQDKTGIRVNITVVPYGRDQNIKLMSSFLAGGSQYDIYVIDCVEVPQYAEAGWALTI
                     DEYVTPEMKKDLLPFAADGMMYKGHWYGLPWASEWKSFLYNATMLTKAGYKDPPKTWT
                     QFVNISKKLQSSGMVKYATAWSWAGKECLICDFAAIAASFGGEFFDEAQNPLFNKGAS
                     VRALQWMVDSVYKEKITNPASIMWTETDVNKAMEAGDIAFGLRWGLPLVPLNDPSRSK
                     TVGQWKIALMPSVDGKHPYTVSGPMGWAISFGTKHKKEAMDFIFFMADKEGAKLAAID
                     QGVVPGWASLYKDPELVKAVPQLDTMLEQGKFVVNRPRVPWYHDFSTMFAVEVNSALA
                     QQKTPQQALNDAYQKTLQIKKDYEARSKK"
BASE COUNT         1475 a         1092 c          984 g         1351 t
ORIGIN      
        1 ggatgtatat cccttatcac ctgaatagat ccagacgata tgactgccag gttccagatt
       61 cagattatcc acttccatca actccagtga tataatagcc agccatcctt agcatgcata
      121 cccctcctct tttaaagacc tccatatatt ttttatcggg acaaataaga ttttatcata
      181 aatatgaata ttcggcaaaa attataattt taaaactcta agatattttt aagcttattc
      241 ttatatttct ccaagtattc tggtataaac ttgagttaac aaataataca aggaggtact
      301 aggatgaaaa gaatagcagg aactatcgga attcttttga tactgacagt atttttaagt
      361 tttacagatg gaggatatac aggcgcacag caggtatcaa gtttattgac tgaggaggag
      421 gtagacagtt tattactgat gagggaagag gaaaagctgg caagagatgt atatacagtc
      481 ttataccaga agtggggatt gagaatattc tccaatattg caacgtctga gcagaggcat
      541 atggattcgg taaaggtact gctggataga tatggcatcc aggatccggt aaaggataac
      601 cagacaggta agtttacctc tcctgaactg gcaaaactgt atgatgagct tgttgaaagg
      661 ggaacgaagt cattgctaga tgctctgatt gtaggagcta acatagaaga acttgatatt
      721 aaagatttga ttgaatcttc atccaagacg gataaggcgg acataaagat ggtttacgat
      781 aatcttaaga ggggttcaga gaatcacctg gaggctttca taaggcagat tacattacgt
      841 ggcggaagct atacatcgaa gtatataaca gagtaaaata tattctgggc agggatgatg
      901 tacagtctct ccctgccatc taccggatgg ttaatctcta taaaggctca ctgcaccttc
      961 atcaattttt atccatgttc cctgcttata atgtatgagt tcatattctc caattaaggt
     1021 atctccgtct ccagtacatc cgtatccatg ggaaaaattc atcagaaact ggtatctctc
     1081 tccagtatat gcatttagat gtatattgga tgttgccagg gcaattgcgt ttaaagcatt
     1141 agcaacatta gtatctgcca cggtaccttc tgcgatcatc tttgcctcaa atggtcctgt
     1201 gcttccacct ataatatctg cacttacagg actgatctga taagaaccaa agatgtagtt
     1261 tattaccacc actcagccga cgaaattatt agccacaccc tgaatcagat cagtgatggt
     1321 aaccgtacct gaaacagcag ggtttaaact gcttgtcagc gtaccactcc agtcaccgct
     1381 gaagtttggg gtgctgcatc ctccaatcgc cccaaggaga aagaaccctg taactatcag
     1441 tagaaaaatt tcttcttcat cgtcactcac ctcctatcct tttactgctc cgctagttaa
     1501 tccctgaatt atgaatctct gaaatatcag tgctataagt atgggcggta cagaggctat
     1561 catccctgct gcacacatga gtccataatc aacagtgaac cgccccataa attctgctat
     1621 agccaccggc atggtttttg atgataaact gctggtaaaa actaatgcca tcagaaactc
     1681 tccccaggaa gagataaagg taaatatccc tgtggtaaaa agggctggtc cagaaagtgg
     1741 taagactatt ttaaacagta cctgggaggg agaacatcca tctatcaggg cagaatcctc
     1801 tatctctctt ggaatcgtct ggaaataccc tctcaggatc catatagcca tagggagctg
     1861 tagagcagag tacacaagaa taagtagaaa cttggtatca agtagatgca ctctggatgc
     1921 aataacatag agggatgtaa ttaagatgac tggtggaatc atctgggcaa agagcagggt
     1981 aaagatcacc cactgtcctg aaagaacatt caacctcact atggcatagg cagccagaga
     2041 cccgaggaag acagatataa gtgccacagt aatacttata aggctactgt taaagagact
     2101 cgccctgaat gtggtggttg ttcctatctt ttcaaaaaaa atactgtgat agtaatcaag
     2161 atttatcgat gatgggaacc agcgtggagg gacacgtgtc aggtctacct caggtgatat
     2221 acttgtttga aatgtccacc agagaggaag caatgtccat atcattgcta aaactataag
     2281 cacaccatct gtaaaggttt ttatcttact gttatgcatt atgcctcctc catctggaat
     2341 agtctggaat agataaatac aaggaccaga ataatagcca ggaggatata tgacatggcc
     2401 gcccccttac caaactgtaa tgaatcaaat actgtagaat acataagata acctacaacc
     2461 tgtgtgctgc ctccaggacc acctgctgta agggcataaa ctaggtcgaa gatcttgaag
     2521 gtggctacag tcttgattac tatcagaata aatgtcattg gctttataag gcggaaggta
     2581 atcttcatga acatctgcca gggggtagcc ccatcaacct ccgatgcctc atagagctct
     2641 tttggtatag tctggagggc agccagatac ataataatgg cgaatggggt tccttcccat
     2701 atgttggcaa gaatcaccat atgaatagcc agggttgggc tggagagcca ggggatataa
     2761 tcctttataa gcccaagctg gaataataag ccattgagaa aaccataatc agaattgtat
     2821 atccatttcc acataagagc cttaacaata ctcggaagga catagggaaa gagaaccagt
     2881 gccctggcta tccctcgcag cctgaactcc ttgtttaata ggagtgctat cccaagcccc
     2941 aggctcatcc ccactaggat atcgtataga acgaaatagg cggttcttcc aaacgcccac
     3001 caccattgtg gatttttaat tgtctctata taatttttca atcccaccgg agtaaggaca
     3061 agagttccgg gtccaacaaa cgtctcaaaa aaacttaaat acccggagta gagtattgga
     3121 tatacaagaa cgactattat aatgaccgct gatggaagta tcaacagata accaagtgtc
     3181 tttctactca taccaatctc ctggataggg ggtgccttac aggcaccccc ttattaacta
     3241 tttcttggaa cgtgcttcgt aatctttctt tatctgtaga gtcttctgat aggcatcatt
     3301 aagagcctgt tgaggagtct tctgctgggc tagggctgaa ttcacttcca ctgcaaacat
     3361 ggtagaaaaa tcatggtacc agggaactct gggtctattc accacaaact taccctgctc
     3421 aagcatggta tcgagctgag gtacagcctt aaccagctcg ggatctttat agagggaagc
     3481 ccagccagga acgacaccct gatcaatggc tgccagttta gcaccctcct tatcagccat
     3541 aaagaatata aagtccatag cctctttctt atgttttgtt ccaaaactga tcgcccatcc
     3601 cataggtcct gacactgtat acggatgttt gccatctaca gatggcataa gggctatttt
     3661 ccactgtcct actgtcttgg acctggatgg atcattcaat gggacaaggg gtaaacccca
     3721 tctgagacca aatgcaatat ctcctgcttc catagccttg tttacatcgg tctctgtcca
     3781 cataatagag gcagggttgg ttatcttttc cttgtataca ctatccacca tccattgaag
     3841 tgcccgtaca gatgccccct tgttgaagag aggattctgt gcctcatcga agaattctcc
     3901 accaaaagag gcagcaatag ccgcaaaatc acatatcaaa cattctttgc ctgcccatga
     3961 ccaggcggtg gcatatttta ccattcctga gctctgaagc ttcttggaga tatttacaaa
     4021 ctgtgtccag gtctttgggg gatctttata cccggctttt gtcagcattg tagcgttata
     4081 gagaaaagac ttccactctg aagcccaggg aagaccatac cagtgtccct tatacatcat
     4141 cccatcagca gcaaacggta gtaggtcttt tttcatctcc ggggtaacat actcgtctat
     4201 cgtcagagcc catccagcct ctgcatactg aggaacctcc acacagtcta tcacataaat
     4261 atcatactgg gatcctccag ccaagaaaga ggacataagc tttatgttct ggtctctacc
     4321 atagggaacc accgtaatat ttacccttat ccctgtctta tcctgaaact gctgcaccct
     4381 cttcaaaaga aaatccacat caggtccgct gatggtattc agctcaattt cctctccaaa
     4441 gccgaaagaa aagatggcaa aagagagtat cgccaccagt accgatacta aaattttttt
     4501 catcaccaac tacctcctct cagaattatc ttaatttttc tccagtctat cctcattggt
     4561 aaaccctttc catcacctcc ttcccaatca gacgaggata aaccagagat atgagttttc
     4621 ttaatattta ttatacacca taattcgggt ggaacaagat tcgtcagggg tccaggtgga
     4681 tatacctgga acttttccca ggctaaaaat attaattctt ctaacctctg tgaactccta
     4741 catgaaattt atctacccac taccagtcac ctatcacgcg ttaatgcttt tgaaggatgt
     4801 taattcctgt tgctgcaata taccccatcc gggtattgac agatatttcc aaatagatta
     4861 ttatatgaat gtcgcaggtt tccgaagggt ttttaagatt ac
//
LOCUS       DAOTKU010000065         4899 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_15117_length_4899_cov_3.587325, whole genome shotgun sequence.
ACCESSION   DAOTKU010000065 DAOTKU010000000
VERSION     DAOTKU010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4899
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15117_length_4899_cov_3.587325"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..834
                     /locus_tag="P5310_02515"
     CDS             <1..834
                     /locus_tag="P5310_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012582793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRU32269.1"
                     /translation="NNRTINLSKALKVYSTIYGSEDWSFNSIEDLLGLDNPEFCLKKV
                     ADFIERYNLKESLYVESFKTLSPIIRPSKIIALGRNYTEHARETGYNPPEEPIFFSKA
                     VSAIIGPEDAIVYPEWLTRVDPEAELGLVIGKRAKNVSKEKAMEYILGYTIINDVTAR
                     DMQTEDLKIANPWFRSKSFDTFCPIGPYLVLKGYIRDPHDLNIELRVNGTTRQKDNTR
                     NLIFKIPEIVSYITLHMTLEPGDIIATGTPSGIAPVYPGDTVEIEIENIGILRNTVIR
                     G"
     gene            978..1391
                     /gene="speD"
                     /locus_tag="P5310_02520"
     CDS             978..1391
                     /gene="speD"
                     /locus_tag="P5310_02520"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HRU32270.1"
                     /translation="MKTLGVHIIVELSGCQANFLDSVEKIKELMIKAAILANAHIKEA
                     VFHKFDPVGVSGVVVIAESHISIHTWPEYGYAAVDIYTCGESTDPWKACEIVAEELKA
                     QSVYVTQINRGIPTENKFIHTLHTTEEVIGYAEST"
     gene            1375..2313
                     /gene="speE"
                     /locus_tag="P5310_02525"
     CDS             1375..2313
                     /gene="speE"
                     /locus_tag="P5310_02525"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HRU32271.1"
                     /translation="MPKVHNWQWFLESVVPSEYHVHGIKRSLIVRETQYQLAEILESY
                     QFGKMLVLDGESQSSERDEYIYHEVLVHPAMVLADNPKRVLLLGGGEGATLREILRYP
                     IERAVMVDIDEEVIELCKEYMPNLSAGSFADPKAEVIIGDARAYVENTEEKFDVIISD
                     LTEPLEEGPASKLFTKEFYNAIKSRLSENGIFVTQALSVSVINNEAHTAIYNTLKQIF
                     PVVRSYHAYIPLYDSDWGFVMGTLGKDPVKIKEEEINERLERDGLTDLKYYDGETHRY
                     IFSLPKDLRNAINMETVIIEDNKPLLIARRERFFGI"
     gene            2303..3127
                     /locus_tag="P5310_02530"
     CDS             2303..3127
                     /locus_tag="P5310_02530"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HRU32272.1"
                     /translation="MESDSVKLVILDIDGVVIRGKTPIPEAIEGIKFLKENNCRVVYL
                     TNNSTRPRQRVLELLDNMGINVSVSDIFTSSYLTALYLSKEISKDSRIFILGEEGIVL
                     ELLEVGFRNIIWTNYDPPIDYVVVGMDRNFTFQKLTCAQHAIMSGARFIATNTDVTFP
                     VENGVIPGGGSIVKAVETASGIKPVVIGKPETLGLRLMLEQLGNVDPEEALFVGDRIE
                     TDLIAGKKLGCKTGIVTSGISKRDDLERLPEKLKPDFLGENLLDLLQGGSKYGDRI"
     gene            3111..3749
                     /locus_tag="P5310_02535"
     CDS             3111..3749
                     /locus_tag="P5310_02535"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRU32273.1"
                     /translation="MEIEYNGHSCFTIKTSLGTIVTDPFGEEIPYPVRELRADIVTVS
                     HEHFDHNAVSRVKGSPYVIKTVGEHKHNGILIRGFQAFHDKNKGSQRGQTVIFIIGAE
                     NLKVCHLGDLGELLDESQIKMLGDIDILLIPVGGFFTIEPEEAKILAEKIKPRIVIPM
                     HYKTDYIKDWNIKPVEEFLREISFPVKRINSSKVTINEEDKPLSTEVYLLKI"
     gene            3746..>4899
                     /locus_tag="P5310_02540"
     CDS             3746..>4899
                     /locus_tag="P5310_02540"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012980399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HRU32274.1"
                     /translation="MKLIFVTGGVVSSLGKGITAASVGRILKARGFNVTIQKLDPYLN
                     VDPGTMNPYQHGEVFVTEDGAETDLDLGHYERFIDSNMTKENNVTAGIVYWSVLNKER
                     KGEFLGGTVQVIPHITNEIKERITNLGRNGYDIVIVEVGGTVGDIESLPFLEAIRQLK
                     KDIGKENVMYIHVTLVPYLKTSGEMKTKPTQHSVKELRSIGIQPDMIFCRTDRELAQE
                     VKDKIALFCDVDSNAVIDIPDMPCIYEVPLHFERSMVGGIITETLNLENKTPDLRDWE
                     ELVNRAKNPIKDVVIGVVGKYIELRDAYISVTSALEHSGIWEDTKVNIKWIEAECLEE
                     EGTEPLEDVDGILVPGGFGQRGIEGKIKAVKFARENKIPFFGICLGMQCAV"
BASE COUNT         1747 a          752 c         1104 g         1296 t
ORIGIN      
        1 aataatagaa caataaacct ttccaaggca ctaaaagtct attctaccat atacggaagc
       61 gaagactggt ccttcaattc aatagaagat ttacttggcc tggataaccc ggaattctgt
      121 ctgaaaaaag ttgcagattt tatagagaga tataatctga aagaatctct ctatgttgaa
      181 tcttttaaaa cactttcccc aataataaga ccatcaaaga taatagcttt aggaaggaat
      241 tatacagagc atgccagaga aaccggttat aatcctcctg aagagccaat attcttcagt
      301 aaagcagttt cagctattat agggccggag gatgcaatag tataccctga atggcttacc
      361 agagttgatc cggaggcaga gttggggtta gtaataggga aaagagctaa aaatgtatct
      421 aaagagaaag ctatggagta tattttggga tataccataa taaatgatgt aaccgccaga
      481 gatatgcaga cagaagatct taaaatagcc aatccatggt tcaggtccaa aagtttcgat
      541 acattctgtc cgataggtcc gtacctggtc ttaaaaggtt atataagaga ccctcatgat
      601 ttaaatatag aactcagagt aaatggaact accagacaga aggataatac cagaaatctt
      661 atatttaaaa tccctgagat agtatcctat ataactctac atatgacact tgaacctgga
      721 gatataatag ctaccggaac tccttcaggt atcgcccctg tatatcctgg agatactgta
      781 gaaattgaga tagaaaacat aggtatttta agaaatacag taataagagg ctaaaaagtg
      841 gatctatccc aggtatagcc ttcaggatat tgcatttttt tctaaaaaac tgtatagtta
      901 taaactgtgt aagggtagta tatctacctg agaataaaaa actataaaac tatagagaaa
      961 ggggttggga gcttgagatg aaaactctcg gggtccacat aatagtggag ctttctggat
     1021 gtcaggcgaa ttttttagac agcgtggaaa agataaaaga acttatgata aaagcagcta
     1081 tattagccaa tgctcatata aaagaagcgg tgtttcataa gtttgatcct gttggggtaa
     1141 gtggggttgt agttatagca gaatctcata tctctataca tacctggcca gaatatggat
     1201 atgcagctgt agatatatat acctgtgggg aaagtacaga cccatggaaa gcctgtgaga
     1261 tagttgccga ggaactaaaa gctcagtcag tctacgtaac gcagataaac agagggatac
     1321 ctactgaaaa taaatttata cataccctgc ataccactga ggaggtgatc ggatatgccg
     1381 aaagtacata actggcaatg gtttcttgaa tccgtagtgc caagtgaata ccacgttcat
     1441 ggcataaaga gaagtttaat agtcagggaa actcaatacc agctagctga aatattagaa
     1501 agttatcagt ttggtaaaat gctcgtttta gatggagaaa gtcaatcatc tgaaagagac
     1561 gaatacatat atcatgaagt attggtacat ccagcgatgg tcttagctga taatccaaaa
     1621 agagtcttac tccttggtgg aggagaaggg gctactttaa gggagatact ccgatatccg
     1681 atagaaaggg ctgtaatggt cgatatagat gaagaggtta tagagctttg taaagagtat
     1741 atgccgaatc tatctgccgg gtctttcgca gacccaaaag cagaagttat tatcggagat
     1801 gccagagctt atgtggaaaa tactgaagaa aaatttgacg ttataatcag tgatctcaca
     1861 gaaccattag aagaaggacc agcatctaaa ttgtttacta aagaattcta taatgccata
     1921 aaaagtcgtc tgtctgaaaa cgggatattt gtaactcagg cattgagtgt ctctgttata
     1981 aataatgagg cacatacagc gatatataat acgttaaaac agatatttcc agttgtaagg
     2041 tcatatcatg catatattcc tctgtatgat tctgactggg gattcgttat gggaaccttg
     2101 gggaaagacc ctgtcaaaat aaaagaagaa gagattaatg agagattaga aagagatggc
     2161 ttaacagatc taaaatacta cgatggagaa actcacagat atatcttcag tctaccaaaa
     2221 gatcttagaa atgccatcaa tatggaaact gttattatag aggacaataa acctctttta
     2281 atagcgagga gagagaggtt ctttggaatc tgacagtgta aaattagtca tattagatat
     2341 agatggagtg gtaataagag ggaaaactcc aatcccagag gctatagagg ggataaaatt
     2401 tctaaaagaa aataattgca gggttgttta tctcaccaat aactctacca gacctcgaca
     2461 gagagtatta gagttattgg ataatatggg gataaacgtc tctgtatctg atatatttac
     2521 ttctagttat cttacagcgc tatacctctc aaaggagatc agtaaagata gtagaatctt
     2581 tattttagga gaagaaggta tagttttgga attattagag gtaggattta gaaatataat
     2641 ctggacaaat tatgatccgc caattgatta tgtggttgtt ggtatggata gaaactttac
     2701 gtttcaaaaa cttacctgtg cacaacatgc gattatgtcc ggagctaggt ttattgcaac
     2761 aaatacagat gtaacctttc ctgttgagaa tggagtaatc cctggaggag gaagtattgt
     2821 aaaggctgtt gaaacagctt ccgggataaa accagtagta ataggtaagc cagagacact
     2881 gggattaagg cttatgctgg aacaacttgg taatgtagac cctgaagagg cgctttttgt
     2941 tggagaccgc attgagactg atctgatagc agggaaaaag ctgggatgta agacagggat
     3001 agttacgagt ggtatctcta aaagagacga tttagagaga ctcccagaga aacttaaacc
     3061 agattttttg ggagaaaatc ttttagacct tttacaagga ggaagtaaat atggagatcg
     3121 aatataatgg gcattcttgc tttactataa aaacctcact tggaacgata gtgaccgacc
     3181 catttggaga agaaatacca taccctgtaa gagaactcag ggctgatata gtcactgttt
     3241 ctcatgagca cttcgaccac aatgctgtaa gcagggttaa aggaagtcct tatgtgatca
     3301 agactgtcgg agagcacaaa cacaatggga tactgattag aggctttcaa gccttccacg
     3361 acaagaataa gggtagtcag agaggtcaaa cagtcatctt tattataggg gcagaaaatc
     3421 tgaaggtatg tcatctaggt gatctgggtg aactactgga cgaatctcag ataaaaatgc
     3481 ttggagatat agatatctta cttatcccgg ttgggggatt ctttaccata gaacctgaag
     3541 aggctaagat tttggcagaa aagataaaac ccaggatagt tataccaatg cactacaaaa
     3601 cagactatat aaaagattgg aacatcaaac cagtagaaga atttctcaga gaaatctctt
     3661 tcccggtgaa aagaataaat tccagtaagg taacaataaa tgaggaagat aaacctctat
     3721 caacagaggt ctatctattg aagatatgaa gcttatattt gtaaccggtg gagtcgtttc
     3781 ttctttaggg aaagggataa ccgctgcttc tgtaggcagg atattaaagg cgagagggtt
     3841 taacgtcacg atacaaaaac tggatccata tttaaatgta gatccaggca ccatgaaccc
     3901 ctatcaacat ggggaggtct tcgtgaccga agacggagca gaaactgact tggatttagg
     3961 tcattacgaa agattcatag actcaaatat gacaaaggaa aacaatgtta ctgccggaat
     4021 agtatactgg tcagtcctta acaaagaaag aaagggagaa tttctaggtg gaacagtaca
     4081 ggtaattcca cacataacaa acgagatcaa agagagaata actaatctgg gaagaaatgg
     4141 ctatgatata gtaatagtag aagttggggg aacagttggt gatatagaaa gtctcccatt
     4201 tctggaggca ataagacaac tgaaaaaaga tatagggaaa gagaatgtaa tgtatataca
     4261 tgtaactctg gtaccatatc tcaaaacttc aggagagatg aaaactaaac ctactcaaca
     4321 tagtgtcaaa gagttgagaa gtataggtat acaacctgat atgatattct gtaggactga
     4381 cagagaattg gctcaggaag taaaagataa aatagcatta ttctgtgatg tagattcaaa
     4441 tgcagttata gatattcctg atatgccctg catctatgag gttcctctac acttcgaacg
     4501 ttcgatggtt gggggtataa taactgagac cttaaatttg gagaataaaa ccccagacct
     4561 tagagactgg gaagagcttg taaatagagc aaaaaatccg attaaagatg tagttatagg
     4621 agttgtaggt aaatacatag aactaagaga tgcatatata agtgtcacat ctgcactgga
     4681 gcacagtgga atatgggaag atactaaagt aaatataaaa tggatagagg cagagtgttt
     4741 agaggaagaa gggaccgagc cattagaaga tgtagatggg atattggttc caggtggatt
     4801 tgggcagaga ggtatagagg gaaaaataaa ggcggtaaaa tttgcaagag aaaataaaat
     4861 accgtttttt ggtatttgtc tgggtatgca atgcgcggt
//
LOCUS       DAOTKU010000066         4898 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_15124_length_4898_cov_3.937642, whole genome shotgun sequence.
ACCESSION   DAOTKU010000066 DAOTKU010000000
VERSION     DAOTKU010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4898
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15124_length_4898_cov_3.937642"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(126..701)
                     /locus_tag="P5310_02545"
     CDS             complement(126..701)
                     /locus_tag="P5310_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HRU32275.1"
                     /translation="MRLKGKKIAILVSNEFEDIEVFYPFLRLSEEGAWVTFGIYRVGF
                     HPRPALPDKPVTGRFGLTVPPMFHREGYRYDVKDATELLPEDYDAVVIPGGFSPDYLR
                     REPRITGFVRKMYEQGKVVAAICHGPWLIISAKIAKGKKIAGVTPIKDDIENAGATFV
                     DEPVVVDDNIITSRVPDDLPEFCQAIIYALS"
     gene            852..1322
                     /locus_tag="P5310_02550"
     CDS             852..1322
                     /locus_tag="P5310_02550"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034023 - 5-(carboxyamino)imidazole
                     ribonucleotide mutase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HRU32276.1"
                     /translation="MKAIIIMGSKGDLDWSKKIGEYLKKFDIPYIMRIASAHKVPLKV
                     LDIIEEYENEDVVFITVAGRSNALSGFIDAQTSKPVIACPPYGDKFAGVDIFSTLRMP
                     SGVAPLTVLEPEQAAIAACKIFAIKYKELEEKIKKYQKEKREEIEIDDENIKNL"
     gene            1457..3409
                     /locus_tag="P5310_02555"
     CDS             1457..3409
                     /locus_tag="P5310_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012310589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRU32277.1"
                     /translation="MRIPKILVILALCAGILVSSIPLIFGAEKVEPGEYYNLSDYEKL
                     TGKKITRFAESPQLAELVKQGKLPPLEQRIPKNPLVVTPYEEIGQYGGTWRRTWSGLS
                     DSAGPYKLCSEHLVMFNKNGTKILPNVAESWKVSRDAKTYTFKLREGIRWSDGTPLTT
                     EDVVFWYEDIILNKDLTPTIPNWLTSGGKPLKLEKIDTYTFKVEFEEPNALFLISIGK
                     MGGGHTFFAPKHYLKQFHPKYTPKEKLDELVKKAGLQNWYQLFGNMNDFLQNPDLPVI
                     YPWKATNLPTATLQIMERNPYYWKIDPEGNQLPYIDRITHTLLGSAGETAVLKAISGE
                     VDMQERGLVIDNYTLLMENRTKGNYRVLRWPQGTGASPAILLNQNVKDPVLRKIFEDR
                     RFRIALSLAINREEINQLFYLGLGEPRQASIISGAPFYDPEWEKAYAEYDPKKANEYL
                     DKMGLTKRDKDGFRLRPDGKTLILTIEYSGDRPYMEVIKKYWEAIGVKVFLKPLERSL
                     YVTRAQAGEIEVGVWGFDRNVAILSDPGHLLGTVVEAPWAPLYATWYNTGGRGGEEPK
                     GDIKRIYELWDKVKSTIDEKQRDRYFKEIINLHKKNIWMIGIVGEVPQLIVAKNNFRN
                     IPDGLLWDDNIRSPKNARPEQFFFKK"
     gene            3563..4219
                     /locus_tag="P5310_02560"
     CDS             3563..4219
                     /locus_tag="P5310_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32278.1"
                     /translation="MDEIEACVKYAPVIMFDEREPFLPMRVGYTIFRDRVSSSPSFPR
                     EVILKESEDFCIEYAIFWDWDIGHLYELEHIWVYTDKNKNISRVDASWHGNFNSMDNI
                     EIKGETHPVLYSQPGKHAFAPDPSWFEPRERFILPCTQETGISGLLITNLFKGKMTKT
                     IEDDELVLKYLTRFAFTPSFNFTKEFHFDSSYFYPWEEVYEWIPRRIKEILENIKKEV
                     "
     gene            4227..>4898
                     /locus_tag="P5310_02565"
     CDS             4227..>4898
                     /locus_tag="P5310_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013466291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HRU32279.1"
                     /translation="MKGLIVTAEWNPKPGYTVSDFEKTTGKAITGNSIWKNPRLELKE
                     VPDPTPGPKDVVLKVRACGVCGSDVHFYETDSDGYMLYPGLTKFPCVIGHEFSGEVVA
                     AGKEVKDLKIGDMVTAEEMIWCGECIPCRNGYPNQCLNLEEIGFTINGAFAEYIVIGA
                     KYCWKIDEIASRYGDKEKGFEAGALVEPTSVAYNGMFVRAGGFKPGGYVVVFGAGPIG
                     LASIAL"
BASE COUNT         1665 a          853 c         1054 g         1326 t
ORIGIN      
        1 gaaatcataa acttatcgac tacagaggag acagagccac gaaagaaacc ggacgtctat
       61 atatagttat gacctttcgt gtaccatttg agcataaatt agagttctct tatggcgatg
      121 tatagtcaag agagagcata aattatggct tgacaaaact ctggtaagtc gtcgggaact
      181 ctgctggtaa ttatattgtc atccactact acgggctcat ctacaaaggt cgccccagcg
      241 ttttctatat cgtctttgat aggagttacc ccagctatct tcttaccttt agctatcttg
      301 gcagatatta taagccacgg tccatgacat atagcagcta caactttccc ctgttcatac
      361 atctttctaa caaatccagt aattctgggt tctcttctca gataatctgg agaaaatcct
      421 cctggaatta ccaccgcatc ataatcttca ggtagtaatt ccgtagcatc cttcacatca
      481 tatctgtatc cttccctatg gaacattgga ggaacggtca gaccaaacct cccagtaaca
      541 ggcttgtcag ggagagcagg tcttggatga aagcccaccc tgtatatccc aaatgtcacc
      601 cacgctcctt cttcgctaag tctaaggaaa ggataaaata cttctatatc ctcaaactca
      661 tttgaaacaa gaatagctat cttcttcccc tttaatctca taacaggatc accctcctag
      721 tctctaatat aaaactattt taatgatagg atattaaaat actatcgtca agagtaatgc
      781 cagggcattt ctgttgacac aagaaaggtc gttctctata ataactcaaa aatcaaggaa
      841 agggagacac gatgaaggct attataatta tggggtccaa aggagatctt gattggagta
      901 aaaagatagg agagtatcta aagaaatttg atatcccgta cataatgagg attgcctcag
      961 ctcataaagt tcccctgaaa gttctggaca taatagagga gtatgagaat gaagatgtgg
     1021 tttttataac agtagccggg agaagtaatg cattgagtgg ttttatcgac gcacaaactt
     1081 caaaaccagt tattgcctgc cctccttatg gagataaatt cgccggtgtt gatatattct
     1141 ctacactgag aatgccatct ggagtcgccc ctctaacagt tctggaacca gaacaggcag
     1201 caatagcagc ttgtaagatt ttcgccatta aatacaaaga acttgaggag aaaattaaga
     1261 agtaccagaa agaaaaacgg gaagaaattg aaattgatga cgagaacata aagaatttat
     1321 agagtggaat atgctatctc caacagagaa ttgaagtacc ctttgggtat tgtttttttg
     1381 agtttttaat ggtataattt tttcgtaaaa aataaataat gttttgtata gtaaaactaa
     1441 aaggagggtg agaaagatga gaattccaaa aatcctggta attttggctt tatgtgctgg
     1501 tatacttgta agttcaatac ctctgatttt tggggcggaa aaggtagagc caggggaata
     1561 ctataacctt agtgactatg aaaaattaac cggtaagaaa atcacaagat ttgctgaatc
     1621 tcctcaattg gcagaactgg taaaacaggg aaaacttccg cctttagaac agagaatccc
     1681 taaaaatcct ctggtcgtaa ctccctatga agagattggt cagtatgggg gaacgtggag
     1741 aagaacatgg agtgggttat cagattctgc tggaccatac aaactctgca gtgaacatct
     1801 tgtaatgttt aataaaaacg ggactaaaat tctaccaaat gtagcagaaa gctggaaagt
     1861 atcaagagat gctaaaacat acaccttcaa attaagggaa ggtattagat ggtcagatgg
     1921 aacgccttta acaaccgaag acgtagtatt ctggtacgaa gatattatac tgaataaaga
     1981 ccttactcca actataccta actggcttac ttccggagga aaacctctca aactagaaaa
     2041 gatagatacc tatacattta aggtagaatt tgaagaacca aatgcattat tcttaatatc
     2101 tatagggaaa atgggaggag gacatacatt ctttgctcca aaacattatc tgaaacagtt
     2161 ccatcccaag tacactccaa aggaaaagtt agatgaatta gtcaaaaagg ctgggctaca
     2221 aaactggtat caactatttg gtaacatgaa tgattttctt caaaatccag atctacccgt
     2281 tatatatcct tggaaagcaa cgaatcttcc aactgctact ctccagataa tggagagaaa
     2341 cccatactat tggaagatag accctgaagg aaatcagctg ccatatatag acagaataac
     2401 tcatacatta ttaggatctg ctggagagac tgcagtacta aaagctatat caggcgaagt
     2461 agatatgcag gaaagagggc ttgttattga taattacaca ttacttatgg agaaccgaac
     2521 taaaggaaat tatagagtat tgagatggcc acagggaaca ggagcaagtc ctgcaatatt
     2581 actcaatcag aacgtgaagg atccagtttt aagaaaaata tttgaagacc gtagattcag
     2641 gatagcattg tcacttgcta taaatagaga agaaattaat caacttttct atctgggatt
     2701 gggtgaaccc agacaggctt ctataataag cggcgccccc ttctatgatc cggaatggga
     2761 aaaggcttat gcggagtatg atcccaaaaa ggcaaatgaa tatcttgata agatgggact
     2821 gacaaaaaga gataaagatg gcttcagact aagaccagat ggcaagacat taattctaac
     2881 catagagtat tcaggagaca gaccatatat ggaagtaata aaaaaatact gggaagccat
     2941 aggagtaaag gtatttctaa agccattaga acgttctctc tatgtaactc gagctcaggc
     3001 cggagaaata gaagttggag tctgggggtt tgatagaaat gtggcgatac tctctgatcc
     3061 agggcatcta ctcggaactg ttgtagaggc tccctgggca cctctctatg ctacatggta
     3121 caacactggg ggtagagggg gagaagagcc caagggagat attaagagga tctatgagct
     3181 ctgggacaag gtaaagtcca ctatagatga aaaacagaga gatagatact ttaaagagat
     3241 tataaactta cacaagaaga atatctggat gattggtata gtaggagaag ttcctcaact
     3301 tatagtagct aagaataatt ttagaaatat acctgatgga cttctatggg atgataatat
     3361 acgatcaccc aagaatgctc gtccagaaca attcttcttt aagaagtaag actcagtttt
     3421 attaaagccc tggtattccg ccagggcttt aatgtttcct gaaggattaa accagagtta
     3481 taaccaccgt acaatccatt gacattatta atagtttatc ttataatttc tttagaaaac
     3541 attttcttag aaggagaatg atatggatga aatagaagct tgtgttaagt atgcccctgt
     3601 tataatgttt gacgagagag agccatttct accgatgagg gttggttata ccatcttcag
     3661 agacagagtc tcctcttccc catctttccc acgggaggta atactaaaag agagcgaaga
     3721 tttctgtata gaatacgcaa tcttctggga ttgggatata ggtcatctat acgaattaga
     3781 gcatatatgg gtctatacag ataagaataa gaacatctca agagtagatg ctagctggca
     3841 tggaaatttc aactcgatgg acaatataga aataaaagga gaaactcatc ccgttttata
     3901 ctcgcaacca ggtaaacatg cctttgcacc ggatccatcc tggtttgaac cgagagaaag
     3961 atttatcctg ccatgtactc aagagaccgg gataagcgga ttacttatta ccaatctgtt
     4021 caaaggtaaa atgaccaaga ctattgaaga tgatgagttg gtcttaaagt atcttaccag
     4081 atttgctttt actcctagtt tcaattttac taaggaattt cattttgaca gtagctattt
     4141 ctacccctgg gaagaggttt acgagtggat tcccaggagg attaaagaga tattagaaaa
     4201 tataaagaag gaggtctaat agaattatga aaggattaat tgttaccgcc gagtggaatc
     4261 caaagcctgg atatacagtc tcagactttg agaagactac tggtaaggcg attaccggca
     4321 acagtatctg gaagaatcca agactggagt taaaagaagt cccagatcca accccaggac
     4381 ctaaagatgt agtattgaag gtaagagcat gtggagtatg cggttcagat gttcacttct
     4441 atgagacaga tagtgatgga tatatgctct atcctggact tacaaaattt ccctgtgtga
     4501 taggacatga attttctgga gaagtggttg cagctggaaa agaagtaaaa gatctaaaga
     4561 taggagatat ggtaaccgct gaagagatga tctggtgcgg agaatgcata ccctgtagaa
     4621 atggctatcc caatcaatgt cttaatctgg aagaaatagg tttcacaatc aatggagcgt
     4681 ttgcagagta tatagttata ggagcaaaat actgctggaa gatagatgaa attgccagta
     4741 gatacggaga taaggagaag ggatttgaag caggagctct ggttgagcct acaagtgttg
     4801 catataatgg tatgtttgta agagcaggag gatttaaacc tggaggttat gttgtagtat
     4861 ttggagctgg accaattgga ctggcttcta tagcttta
//
LOCUS       DAOTKU010000067         4857 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_15263_length_4857_cov_3.760308, whole genome shotgun sequence.
ACCESSION   DAOTKU010000067 DAOTKU010000000
VERSION     DAOTKU010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4857
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15263_length_4857_cov_3.760308"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            65..1282
                     /locus_tag="P5310_02570"
     CDS             65..1282
                     /locus_tag="P5310_02570"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HRU32280.1"
                     /translation="MNEFAYRAREKKTGRWREGSIVADDEATVIRRLQEEDLVVVSVK
                     EKKVTFDINKFLTSLQRVSLRDLVIFTRQFATMLRSGVAMVRALSILSQETKNPRLKL
                     AVEDIRKEVESGTALSQALAKHRDIFPRLYISLVRVGETGGILDTMFDRLAAYYEKNE
                     EIARKIRSASAYPMAIIIMAIVMVIFLLTYVLPTFLNLLISFEAPLPLPTKIVMSLSN
                     VFRDYWYVVFSVVIVLYLLFKMVTSKPKGKELWDKFILKIPVIGRLNVLMVVSRLCHT
                     LSILLSGGIPIVVALEVLKDTMDLTPYMNAIEDAKRMITEGQQLSTCFVKSNLFPPML
                     GYMTAVGEESGLVDEMLERVGVYYDGELDAMLSTLTALVEPILIAFVGGVVGFIVISM
                     YLPIFQIISTLME"
     gene            1282..2016
                     /locus_tag="P5310_02575"
     CDS             1282..2016
                     /locus_tag="P5310_02575"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HRU32281.1"
                     /translation="MREIQLNLTPFKKLISLDSVSSTMDIVFKMSRLGERFFVVSAEE
                     QWAGKGKGNRRWYSDRFSLSFSIAIDGKFLYRQSLLSILAGVIVKKGIVSYLGIPIEL
                     KWPNDIVFKGKKLGGILGENWNEFIIIGIGINVNNLEFPCYLESAISLRQIQGREIDK
                     VELLKSIIKEFSLDFNLFAEGNDILIKEWKKSCTTIGERVKFKSYNNIKEGIVTDIAN
                     DGALLVSLSDGRRKKIYSSDELVTER"
     gene            2039..2851
                     /locus_tag="P5310_02580"
     CDS             2039..2851
                     /locus_tag="P5310_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32282.1"
                     /translation="MREYLRLSDNDLIFVVETLSPQLRDRIDTVREDEDIIMGMLEDD
                     RLFSRVMDDDEIMLKISPKLFFTILMIRARKDLNREYYTVERDRSHRIPIFDVEKVRD
                     LINQDDILDYLIDMLVSFTKTESQTIYYAVKGRRGLRRLKFSDIDVFDMLRLAELVDE
                     DNRFPIYKRIGDICLWTLGIFPEYAMPRSFARPVPSSRAGVRFRMSFEEYEELGRTFY
                     RTASNSYSAKVFGLDRPLSILSEQFTIAEKPLNLISERYLAFRKDRWFISLN"
     gene            3028..3339
                     /locus_tag="P5310_02585"
     CDS             3028..3339
                     /locus_tag="P5310_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32283.1"
                     /translation="MRKIPEDVILSNWYYGSEFNEDINYAKTFIDLEEGGYKQIPAGS
                     NWSFSDNFPNLVDYCIKNISEENLLGFLQTSWLPTTWSYIDGHIDAIDKVKEAKKRTG
                     L"
     gene            3336..4508
                     /locus_tag="P5310_02590"
     CDS             3336..4508
                     /locus_tag="P5310_02590"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRU32284.1"
                     /translation="MNSTRLKRRIFILCWCAYAGAYLCRTNLSIVVPFLIEDLGWDKG
                     TVGLLGSIFFWVYAFGQLINGYIGDRINTKLFIFFSLLLSSVINIVLGFVVSKFIFTI
                     LWGVNGFLLSMLWGPIIRILGLWFPKEESNKIGVGISTSMFAGYLVYWGPIGQFIRNT
                     NWRLAFYIPGILVALFSLCWLIALPSKEEKIDRESGRIPVKQLLSPGLIFIALTCMAQ
                     GTIKESIGLWTPTILSDTARSIPDFTMFIPIMGFSGLLSAGWLNYRFNHREEFTVLLL
                     FSLTLISNILLFLLLGLNVYLTICLLGVSLAILYGANALLLANIPLRFVAYGGGSTVA
                     GFLDFSSYTGSAIASIITGNLATNFSWRSVVLLWCIASLAGILSVYLSQKNIKSQT"
     gene            complement(4516..>4857)
                     /locus_tag="P5310_02595"
     CDS             complement(4516..>4857)
                     /locus_tag="P5310_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019908016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRU32285.1"
                     /translation="ASSYRCLAMINYINMIPPLVNWDNNVDYLKRAASEILGEENCIE
                     AKPILTGEDFTLYLEKKPGCFFWLGSKPTDGKEVSGLHSPKYRIDESCIPLGTAILTK
                     LAYTRIGNIST"
BASE COUNT         1433 a          795 c         1103 g         1526 t
ORIGIN      
        1 attttattaa taatgttaca aatagagaag agatgatgag aatggtaggg gaaagagtca
       61 ggatatgaac gaatttgcat acagggctag agagaaaaag acaggtcgct ggagagaagg
      121 ttctatagta gctgatgatg aggctaccgt tataaggaga ttgcaggaag aagaccttgt
      181 tgttgtatct gtaaaagaga aaaaggtaac ctttgatata aacaaatttc ttacaagcct
      241 ccagagagtt tctctcagag accttgtaat ctttacacgc cagtttgcaa ccatgttgag
      301 atcgggggtt gctatggtaa gagctctcag catcctttct caggagacga agaaccctag
      361 gttaaagctg gctgtggaag acataaggaa agaggtggaa tctggcactg ctctatcaca
      421 ggcactggct aaacatagag atatatttcc aagattgtat ataagccttg taagggttgg
      481 ggaaacaggt ggtatactgg atacgatgtt tgatagactt gccgcctatt atgagaagaa
      541 tgaagagata gccagaaaga ttagaagtgc ttctgcttat ccgatggcta taatcattat
      601 ggctattgtt atggttatat ttttgcttac ctatgtctta ccaacattcc ttaatctttt
      661 aatatctttt gaagccccgc ttccactacc aacgaagata gttatgagtt taagtaatgt
      721 ctttagagac tactggtatg ttgtgttttc agttgtaatt gtcttatacc ttctattcaa
      781 gatggttaca tcaaagccga aggggaagga actctgggat aaatttatcc ttaagatacc
      841 agttataggc agacttaatg ttcttatggt tgtctcaaga ctatgtcata cattgagtat
      901 acttttatct ggtggaatac cgatagtggt tgcgcttgag gtgttgaaag ataccatgga
      961 tcttaccccc tatatgaatg ctatagagga tgccaaacgt atgattacag aaggtcagca
     1021 actctccacc tgttttgtaa agagtaatct ttttccacct atgctgggat atatgactgc
     1081 ggtaggggag gaaagcggtc tggtcgatga gatgctagag agagttggtg tatactatga
     1141 tggtgaactt gatgcgatgt tatccacact cactgcgctg gttgaaccaa tcctcattgc
     1201 ttttgtagga ggggttgtag gttttattgt aatatcaatg tatctgccca tattccagat
     1261 catctcgact ctgatggaat agtgagagaa attcaactaa atctgacccc ttttaagaag
     1321 cttatctctc tggatagcgt gtcttccact atggatattg ttttcaagat gtcccggctt
     1381 ggagagagat tttttgttgt ttctgctgag gaacagtggg ctggcaaggg gaagggaaat
     1441 agaaggtggt attcagatcg cttctccctc tcattctcca ttgcaataga tggaaagttt
     1501 ttatacaggc agtctctttt atctattctt gccggtgtca tagtaaaaaa gggtattgtc
     1561 tcttacctgg gtataccgat agaattaaag tggcccaatg atattgtgtt taaagggaag
     1621 aagctgggtg gaatcctggg agagaattgg aacgaattta taattattgg gattggtata
     1681 aatgtaaata atttagaatt cccctgttat cttgaatctg ccatatccct gaggcagatt
     1741 caaggaagag agattgataa agtagagcta ttgaaaagta taattaaaga gttctcgctg
     1801 gattttaacc tattcgctga aggtaacgat atcttgatta aagagtggaa aaagtcctgc
     1861 accacaatag gtgaaagggt aaagtttaag agttataata atataaaaga aggtatagtt
     1921 acagatatag ctaacgatgg agcgctttta gtctctctca gtgatggtag aaggaaaaag
     1981 atatattcct ctgatgaact ggtaacagag agataaaaat tgaactggag ggaagactat
     2041 gagagaatat ctcaggttat cagataatga cttgatattt gtggttgaga cattatcccc
     2101 tcagctgagg gacagaatag atactgtaag agaagatgag gatatcatta tgggtatgct
     2161 ggaggatgat agattgttct ccagagttat ggatgacgat gagataatgt taaagatttc
     2221 tccaaagttg ttctttacta tacttatgat aagagcgagg aaggatttga atagagagta
     2281 ttacactgta gagagggaca ggtctcacag gattcctata tttgatgtag aaaaggtcag
     2341 agatttaata aaccaggatg atatacttga ctatcttata gatatgcttg tctcttttac
     2401 aaagactgag agtcaaacga tctattatgc tgtaaaaggg agaagagggc tgaggaggct
     2461 taagttttca gatatagatg tcttcgatat gctgcgactt gcagaattag ttgatgaaga
     2521 taatcgattc cctatttata agaggatagg ggatatatgt ttatggacac ttggaatatt
     2581 tccagagtat gcgatgccaa gatcttttgc tcgacctgtt ccatcttcca gggctggtgt
     2641 cagatttagg atgagttttg aagagtacga agagttaggt agaaccttct acaggactgc
     2701 atcgaattca tattcggcaa aggtctttgg tcttgataga cccctttcaa tcctgagtga
     2761 acagtttaca atagcagaaa aacctttaaa tctcatatcg gagagatatc tggcatttag
     2821 aaaggataga tggtttatct ccttaaatta gatgagccct ttctttatcc cgtatgatac
     2881 tgcttcagcc ctaaaaccag catttttaat agtgagagca aaaagctctg gtggaaagac
     2941 ctgtacttct ttattgatat cctggagagg gagaatgttc gtccctggat atgggctgac
     3001 tatatctggc atcacaggga agagtttttg aggaagatcc cggaagatgt tatactgagt
     3061 aactggtatt atgggagtga atttaatgaa gatattaatt acgcaaaaac atttatagat
     3121 ttagaagagg gtggttataa acagattcca gctggaagca attggagttt ttcggataat
     3181 ttccccaacc tggttgatta ctgcataaaa aacatatcag aagagaatct gctcggcttt
     3241 cttcagacct cctggcttcc aaccacctgg tcttacattg atggacacat agatgctata
     3301 gataaagtga aggaggcaaa gaaaaggact gggctttgaa ttctaccaga ttaaaaagaa
     3361 ggatttttat actttgctgg tgtgcctatg caggggcata cttatgcagg accaatctct
     3421 ctattgtggt cccttttctt atcgaagatt taggatggga taaagggacc gtaggacttc
     3481 tgggaagtat tttcttctgg gtttatgctt ttggacagct tatcaatgga tatatcggtg
     3541 acaggataaa tactaagcta tttatatttt tttctcttct cctttcttct gttataaaca
     3601 tagtactggg ctttgtagtt agcaaattta tatttaccat actctggggt gtaaatggat
     3661 tccttttatc aatgctctgg ggtcctatta taaggatttt gggtttatgg tttccaaaag
     3721 aagagagtaa taaaataggt gttggaatat ctacatctat gttcgcaggt tatcttgtat
     3781 actggggacc gataggtcaa tttataagaa atacaaactg gagactggca ttttatatcc
     3841 ccggtatact ggtagctctt ttttctctat gctggcttat agcattacct tctaaagagg
     3901 aaaagataga tagagaatca ggcagaatac cagtaaaaca gttgctcagt ccgggattga
     3961 tatttatcgc attaacatgt atggctcagg gaacgataaa agagagtatt ggtctctgga
     4021 ctccaacaat cctgagtgat actgccaggt ctattccgga ttttaccatg ttcatcccta
     4081 ttatggggtt ttcaggatta ctttctgctg gctggttaaa ttacaggttc aaccatagag
     4141 aagagtttac tgtgttgttg ctattttcct taactctgat ctcaaatatt ctgctgttcc
     4201 tcctgttagg actgaacgta taccttacta tatgtcttct gggtgtttct ctggcaattc
     4261 tatacggagc gaatgccctt ctactggcaa atataccact caggtttgta gcatatggcg
     4321 gtggctcaac tgtggcagga ttccttgatt tttcttcgta tacaggttct gcaatagcca
     4381 gtattatcac cgggaatcta gctacaaact tcagctggag gtctgtagtc ttattatggt
     4441 gtatagcctc acttgcagga atattatcag tatatctcag tcaaaagaat ataaagtctc
     4501 agacttaaaa agtagtcagg tagagatgtt accaatcctt gtatatgcca gttttgtcag
     4561 gattgcagtc cctaagggta tacaggattc atctatccga tatttggggc tatgtagtcc
     4621 ggatacctct ttcccatcgg taggcttgct tccaagccag aaaaaacagc ccggtttttt
     4681 ctccagatat aaagtgaaat cttcgccagt cagtattggt ttagcttcaa tacaattttc
     4741 ctctcccaat atctctgagg cagccctttt cagatagtcc acattattat cccaatttac
     4801 aagtgggggt atcatattta tatagttaat cattgccaaa cagcgataac tactggc
//
LOCUS       DAOTKU010000068         4812 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_15428_length_4812_cov_3.256044, whole genome shotgun sequence.
ACCESSION   DAOTKU010000068 DAOTKU010000000
VERSION     DAOTKU010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4812
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15428_length_4812_cov_3.256044"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(39..1178)
                     /locus_tag="P5310_02600"
     CDS             complement(39..1178)
                     /locus_tag="P5310_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HRU32286.1"
                     /translation="MNRDDAIELHRYLNGKLSVDPKVDVMDKTDLSLVYTPGVGFVSE
                     EIARDKEKVYSYTWKSNTIAVVSDGTAVLGLGNVGPESALPVMEGKALLIKKFAGVNA
                     VPICIRSKSWEDTVHFVLSIAPTFGLILLEDIKAPECFRVEEALDKTLDIPFFHDDQH
                     GTAIAVLAGLINALKLVNKKKENVKIVISGAGAAGTAVTYLLLESGFKDIIVCDSKGI
                     INTKREDLNEDKYKLAQKTNPKGLSGGLGLAMSGADIFIGLSAPGILTGDMIRSMASD
                     PIVFAMANPIPEIDYNDAVSSGAKVVATGRSDYPNQINNLLVFPGLIKGSLLSRKKVD
                     NKIKIKAAQAIADIVSEEELEKGIIVPSPFNPLVVEKVTLAFLAQ"
     gene            complement(1171..2004)
                     /gene="nadC"
                     /locus_tag="P5310_02605"
     CDS             complement(1171..2004)
                     /gene="nadC"
                     /locus_tag="P5310_02605"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013704860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HRU32287.1"
                     /translation="MNEISKIVMMSLEEDIGIGDITTDLTIDENLRGRARVRCKEKAI
                     LCGCEIVKEVFHTIDNSLVLKFLKYDGEEVSPKENVLFIEGKIRSILKGERVSLNFLS
                     HLSGIATVTSNLVKRLPSSIKILDTRKTLPGLRFLEKYAVKVGGGKNHRFGLYDGILI
                     KDNHSIAVGGVKNALELVKRNVPHYMKVEIETATLEDVKEALEGGADVILLDNMDVET
                     IREAIKLIDGRAQIEVSGGINDNNIEQLVDLPVDFISVGAITHSAKSIDFSLTLEETF
                     E"
     gene            complement(2001..3560)
                     /gene="nadB"
                     /locus_tag="P5310_02610"
     CDS             complement(2001..3560)
                     /gene="nadB"
                     /locus_tag="P5310_02610"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014257135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HRU32288.1"
                     /translation="MNNRCDVLIVGSGIAGLYTALNVYDKINVLVITKGDITDSNSYN
                     AQGGIAAAIGKDDSPDIHYHDTIMAGDGLCNPAAVRILVDNAPSIVEDLIRLGVEFDR
                     DENGELDLGREGAHSRRRVLHSNGDSTGKAIMDVLIRHIIERKTPFRDKVMVLELIVE
                     NNICRGVIVWDREREEIYPIYSGVTVLATGGIGQLYPFTTNPYVACGDGLAVAYDAGA
                     ELCDMEFVQFHPTVLKHPSAPSLLITESIRGEGGVLRNIRGERFVPRYHSMGELAPRD
                     VVSRIITEEMEKTNSDYVYLDITHLSRDFILSHFPMIYGECLKYGIDITKDYIPVSPG
                     AHYMMGGIMTNLKGETSIRGLYACGECAGTGIHGANRMASNSLLEGLVFGKIIAQEAS
                     KYVLHNGDIDILEEVTATEKKISDKGILDDYRRRLQDVMWKYCGIKREENGLKELLKE
                     IGILEEEIRDLPVANFRDVETKNMLELAGLIGETAMERRESRGAHFRVDYPYRDDIYW
                     QKHIVKKKEIK"
     gene            complement(3553..>4812)
                     /locus_tag="P5310_02615"
     CDS             complement(3553..>4812)
                     /locus_tag="P5310_02615"
                     /inference="COORDINATES: protein motif:HMM:NF025930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HRU32289.1"
                     /translation="INGSVERDIPADVANHVFDLMEYFAGYGFNKSHTVAYGLLSYQS
                     AYLKAHYPLAYMTALLMSVEGNTAKVASYIQEARRMGIKILPPDINESNIHFTPTNNN
                     IRFGLSAIKNVGESAVEVILEARKKGGKFTSLEDFLRRVDLRKVNKKVIESLIRAGAF
                     DSLGKSRAELLAIFEQYQEKKSTGKGKRKNLFDSEMVNLEPVPAIGEFPMEEILAMEK
                     ELIGLYISDNPLLHYTDELKKRISCNIEELEELDDGVTVTIGGVVSGIKLVDTKNGKM
                     GFLQIEDLSGSIEVVVFPKIFKDINKEINNSTNVFIVEGKIDKKEEEIKVIADRVYLL
                     GKSHYEEKIRSLHIKIKEERCSNNVLFGLKSILSGYSGEMPVIVHIVDDTEICEVALG
                     NEYRIRESGKLIAELKSMFGVANVWIE"
BASE COUNT         1360 a         1204 c          673 g         1575 t
ORIGIN      
        1 aatataaaat ttaaaaataa atttacaatt acaagaattt actgcgctaa aaatgccaga
       61 gtcacttttt ctacaaccag ggggttaaat ggacttggca caataatccc cttctctaac
      121 tcctcttctg aaactatatc tgctatagcc tgagccgctt ttatttttat cttattatcc
      181 actttctttc ttgataaaag agaccctttt ataagaccag ggaaaactag aagattattt
      241 atctggttag gatagtctga tcttccggta gcaaccactt tagcccctga cgaaaccgca
      301 tcgttataat ctatctccgg tatcgggtta gccatcgcaa aaacaatcgg atcagatgcc
      361 atacttctta tcatatcccc tgttaaaatc cctggggctg atagacctat aaatatgtct
      421 gcaccactca ttgccagtcc taaaccaccg gataatccct tcgggtttgt cttctgggct
      481 aacttatatt tatcttcatt aaggtcctct ctttttgtgt ttataatacc cttactatca
      541 cagacaatta tatctttaaa accagattcc agcaatagat aagtcaccgc tgtcccagct
      601 gcacctgcac ctgagattac aatttttaca ttctcttttt tcttattcac cagtttgagg
      661 gcgtttatca atccggcaag tacagctata gctgttccat gctgatcatc atggaagaac
      721 ggaatatcca gggttttatc taaagcttct tctactctaa aacattccgg agcctttata
      781 tcttctaaca gaataagccc aaaagttggc gctatagaca ggacaaagtg aaccgtatct
      841 tcccacgact ttgaccttat acatatagga actgcattta cccctgcaaa tttttttata
      901 agtagagcct taccctccat aacaggaagg gcagattcag gtccaacgtt cccaagacca
      961 aggactgccg taccatcact cactacagcg attgtatttg acttccaggt gtaagagtag
     1021 accttctctt tatccctggc tatctcttca gagacaaaac ctacccctgg agtatatact
     1081 agacttaggt ctgttttatc cattacatcc actttggggt caacagagag tttcccattt
     1141 aaatatctat gtaattctat agcatcatct ctattcaaag gtttcctcca gcgtcaaaga
     1201 aaaatctata gatttggctg aatgtgtgat ggctcctaca gatataaaat ctacaggcaa
     1261 gtcaacaagt tgctctatat tattatcatt tataccgcca gatacttcta tctgcgccct
     1321 gccatctatc agcttaatag cttctcttat cgtctccacg tccatattat cgagcagtat
     1381 cacatccgct ccaccttcca gtgcttcttt cacatcttca agggtagccg tttctatttc
     1441 taccttcata tagtgaggaa catttctctt aaccagctcc agggcatttt ttacccctcc
     1501 aacagctatt gaatgattat ctttaatcag tatcccatca taaagtccaa acctgtggtt
     1561 cttgccccct ccaaccttca cagcatattt ttccaaaaat ctcaaacctg gtaatgtctt
     1621 tctggtatca agaatcttta tagaggaagg aagacgtttt accaggttac tggttactgt
     1681 agctatacct gatagatggg agaggaaatt aagagaaacc ctctcaccct taagtatgct
     1741 tcttattttc ccctctataa ataacacatt ctctttagga gaaacctctt ccccatcata
     1801 tttcagaaac ttcagtacca gagaattatc tatagtatgg aaaacttcct ttactatttc
     1861 acagccacag agtatagcct tctccttaca tcttacacgg gctctacctc tgagattctc
     1921 atctatagta aggtctgtgg taatatcccc aattcctata tcttcttcca aagacatcat
     1981 aactatctta gatatctcat tcattttatc tccttcttct ttacaatatg tttttgccag
     2041 tatatatcat ctctatatgg atagtccact ctaaagtgag ctcctctact ctctcgtctc
     2101 tccatagcag tctctccgat cagccctgct aattctaaca tattctttgt ctcaacatct
     2161 ctaaagttag caacaggtag atctcttatc tcttcttcta atatccctat ctcctttaac
     2221 aactccttta acccattctc ttctctttta ataccacagt atttccacat aacgtcctgt
     2281 aatctacgtc tataatcatc taaaattcct ttgtcagata tcttcttctc tgtagcagtc
     2341 acttcttcta agatatctat atctccatta tgaagcacat atttggaagc ctcctgagct
     2401 ataattttac caaaaaccaa accttcaagc aaagaattac ttgccattct attggcacca
     2461 tgaataccag ttccagcaca ttccccacag gcataaagcc ctcttatact tgtctctcct
     2521 ttaagattgg tcattatacc acccatcata tagtgagctc ctggagaaac cgggatatag
     2581 tcctttgtga tgtctatccc atattttaga cattccccat atatcatagg aaagtgagac
     2641 agtataaaat ctcgggagag atgtgttatg tccagatata cataatcaga gtttgtcttt
     2701 tccatctcct ctgtaattat tctagagacc acatctctgg gtgcaagttc ccccatactg
     2761 tgatacctgg gcacaaacct ttctcccctg atatttctca gaaccccacc ctctcccctt
     2821 atagattctg ttatgagaag agaaggcgca gatggatgct ttagaacagt aggatggaac
     2881 tggacaaatt ccatatcaca caactcagct ccagcatcat acgctacggc taaaccatcc
     2941 ccacaagcta cataggggtt tgtagtaaat ggatataact gtcctatacc ccctgtagca
     3001 agaacagtaa ctccagaata tattggataa atctcttccc tttccctatc ccagactatg
     3061 acgcccctgc agatattatt ctctacgatt aattccagga ccataacttt atctctaaat
     3121 ggagtctttc tctctataat atgtcttata agaacatcca taatagcttt tcctgtagag
     3181 tcaccgttac tgtgcaacac tcttctcctg gagtgagcac cttctctacc taaatctaac
     3241 tctccatttt cgtctctatc aaattcaact cccaatctaa taaggtcttc tacaatactg
     3301 ggggcattat caacaagtat cctcaccgca gcaggattac acaatccatc ccctgccatt
     3361 atagtgtcat gataatggat atcagggcta tcatctttcc ctatcgcagc ggctataccc
     3421 ccttgagcat tatatgaatt actatctgta atatcgcctt tggtaattac aagaacatta
     3481 atcttgtcat acacatttaa agctgtatat aaacctgcta tgccactccc cactataaga
     3541 acatcacatc tattattcaa tccagacatt tgccacccca aacatagatt taagttcagc
     3601 tataagctta cccgattctc taattctata ctcattacct agggctactt cacatatctc
     3661 agtatcatcc actatatgaa caataaccgg catttctcct gaatatccgg agagaatact
     3721 cttaagtcca aagagaacat tgttgctaca tctctcctct ttaatcttta tatgaagact
     3781 tcgaatcttc tcctcgtaat gagatttgcc taatagatag accctatctg ctattacttt
     3841 tatctcttct tccttcttat cgatcttacc ttcaactata aatacattgg tggaattatt
     3901 tatctcctta tttatatctt taaagatttt aggaaatacc accacctcta tactccctga
     3961 taaatcctct atctgtaaaa agcccatctt accatttttt gtgtctacca gttttatgcc
     4021 tgatacaact cctcctatag taactgtaac tccatcatct aactcttcca gttcttctat
     4081 attacaggat atcctcttct tcagttcatc agtatagtgt aacaatggat tgtctgatat
     4141 ataaagacct ataagttctt tctccatagc cagtatctct tccataggaa actcacctat
     4201 agctgggact ggctctaagt tgaccatttc agaatcaaaa agattttttc tcttaccttt
     4261 tcctgtactt ttcttctcct ggtattgttc aaaaatggca agaagctctg ccctactttt
     4321 gcctaaagaa tcaaaagccc ctgctcttat aaggctctcg atcacctttt tgtttacctt
     4381 tctcaggtcc acccttctga gaaaatcctc caggctggta aactttccac ccttcttcct
     4441 ggcttcaagg atcacctcta ctgcactttc tccaacgttc tttatagcac ttaacccaaa
     4501 acggatatta ttattcgtag gggtaaagtg gatattactc tcattgatat ctggaggaag
     4561 gatctttatc cccatccttc tcgcctcttg tatataactg gcaacctttg ccgtgttacc
     4621 ttctacgctc ataagaagtg ccgtcatata tgccagagga tagtgtgcct tcagataggc
     4681 tgactgatac gataataaac cataggctac cgtatgagat ttattaaaac cataaccagc
     4741 aaagtattcc atcagatcaa atacatgatt agctacatct gcaggtatat ccctctctac
     4801 tgaaccattt at
//
LOCUS       DAOTKU010000069         4694 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_15867_length_4694_cov_3.700798, whole genome shotgun sequence.
ACCESSION   DAOTKU010000069 DAOTKU010000000
VERSION     DAOTKU010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4694
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15867_length_4694_cov_3.700798"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="P5310_02620"
     CDS             complement(<1..306)
                     /locus_tag="P5310_02620"
                     /inference="COORDINATES: protein motif:HMM:NF020897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRU32290.1"
                     /translation="MRTKGKTLDNIQSLERALRIIEILAHSEKPLGVTEIGKLIGVHK
                     STAYRLIYTLRQFGYVDQDEDTEKYYLGLKPLELSSIINERLDIKGIIRPYLEELSSK
                     "
     gene            complement(355..2136)
                     /locus_tag="P5310_02625"
     CDS             complement(355..2136)
                     /locus_tag="P5310_02625"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRU32291.1"
                     /translation="MRGEISKTYRELYVYIKPYKLLVFVLFLLTIMLAPARLLLSPVL
                     KFLFDGVFNAQKDYTGAMRNLHTVIFVIIGITILQSIIQYWQIYLQSIITQRVMNDLR
                     VKVYAHLQKLSMEFYQRWHTGEIISRLSNDITLIPQALNAILMDIPLSSLTMVGALGY
                     MIYLNWRLTLFTLIMLPFIASVISFLGNQMIRITRQVQQRFAELVKLLEERIKGMHLV
                     KSFGMEDKEISEFQEVSKENLKVSLESAKIIATQTPIVTLLQTIGLLGVLWFGGIQVL
                     NGNMTSGSLMAFFTALLMVADPIYSISRSFNTISQSAGAIGRIFEIFQVEPSIVEDPN
                     AIIVEPIRGEIEFRDVTFWYKEGEPVIKGVSFHIYPGEVVALVGRSGAGKTTIVNLLM
                     RFYDPQEGVILIDNIDIKKLKLKSLRDQVSIVPQETILFSGTILENIRYGTPYATRDD
                     VIEAARAANAHEFIVKLPRGYDTEIGGDSGTNLSGGQKQRIAIARAFLHNPKILILDE
                     ATSALDSESEALVKDAIIKLMKERTTFIISHRLGFVKEADYIFVIENGEIVERGKHSE
                     LLAKNGLYKSLYERGSELVLEEGESDD"
     gene            complement(2136..2822)
                     /locus_tag="P5310_02630"
     CDS             complement(2136..2822)
                     /locus_tag="P5310_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HRU32292.1"
                     /translation="MRPVECQRESGILSPETLLLKIYYTLYDTFGPQHWWPGESKLEV
                     VVGAILTQNTNWKNVEKAINNLKERGILSVDGILESEGLLPELIKSAGYYRVKTERLI
                     NVMKKIKEYKTLDIFLDLPVESLRSELLSIKGVGEETADSIILYAAGYPVFIVDAYTR
                     RIFSRLGIIRGNEDYGEIQSMVMNNTPNDTYLYNEFHALLVELGKRYCRKIPIHRGCP
                     LSILCKEVNE"
     gene            complement(2940..3974)
                     /locus_tag="P5310_02635"
     CDS             complement(2940..3974)
                     /locus_tag="P5310_02635"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HRU32293.1"
                     /translation="MNITIMESEIREEPEIVGKILNNNINVLLKIKSYLEKRYLDGIY
                     ISARGTSDNASNLGKYLIESMLGYTVALSAPSLFTIYKTPPSLKNRIVIGVSQSGRGE
                     DVCEVIAQGKKDGALCIAITNDPNSPLAEMSDEVILCWAGEEKSVPATKTYLAEIFNL
                     YLFCAILSDRTDILNLLKNDIPLYIKEVIDKSYGIDVTRFHFMDRCIVIGRGFNYPSA
                     IEFSLKLKETSYIFSESFSSADFLHGPLVLVSPQMPIFIFVPSGPSYKHLKEVIGILE
                     EKNTEVFSFSFERLKEERLNFVLPFKVDEVLSPLLFAPAFQIFANRLAVEKGINPDNP
                     RYLKKVTITR"
     gene            4091..4633
                     /locus_tag="P5310_02640"
     CDS             4091..4633
                     /locus_tag="P5310_02640"
                     /inference="COORDINATES: protein motif:HMM:NF013914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009523 - photosystem II [Evidence IEA];
                     GO_component: GO:0009654 - photosystem II oxygen evolving
                     complex [Evidence IEA];
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0015979 - photosynthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PsbP-related protein"
                     /protein_id="HRU32294.1"
                     /translation="MFYKKLWVAVMVLALFTLCVSGAAAQELTEVYSDPDNGFSVSYP
                     ENWLEGELEGALVWLYDEETSSELIVVKEEIPEGFTAKQYADAVGEWLEQNLEEYSEE
                     SLTEGTLSGAPSFTRVYSFSLKGEEATVSIKTLEIYTVKDDYGLAVLCDTTSENFASM
                     EDLFNKMVASFKFLEEEKPN"
BASE COUNT         1407 a          988 c          782 g         1517 t
ORIGIN      
        1 cttgctggaa agttcttcta aatagggacg gattattcct tttatatcca atctttcatt
       61 tataatactg gaaagttcca gcggttttaa tcccagatag tacttctctg tatcctcgtc
      121 ctgatccaca tacccgaatt gccttagagt atatataagt ctataggcgg tactcttatg
      181 aacccctata agctttccta tctctgtaac ccccagaggt ttctctgaat gagctaggat
      241 ttctataatt ctgagagctc tttcgagact ctgtatatta tctaaagttt tcccctttgt
      301 tctcataatc tcaaccctgt ttctgtagaa ttataacaac tatatccata caaatcaatc
      361 atcactttct ccttcttcca gaactaattc tgaacctctc tcatacaaac ttttatatag
      421 accattttta gccagaagtt cactatgttt tcccctttcc actatctctc cattctcgat
      481 aacaaagata taatcggctt cctttacaaa accaagcctg tgagagatta taaatgttgt
      541 cctttccttc ataagtttaa taatcgcatc cttcaccaaa gcctcagact ctgaatccag
      601 agcagatgtt gcttcatcca gaataagtat cttggggtta tgtaagaagg ctctggctat
      661 agctatacgc tgtttctgtc caccagataa atttgtaccg gagtcacctc caatttcggt
      721 atcgtaccct ctgggtaatt tcactataaa ttcatgtgca ttagcagccc tagcagcttc
      781 tataacatca tccctggtcg catatggagt tccatatctt atattctcca ggatagtacc
      841 agagaagagg atagtctctt gaggaactat actcacctgg tctctgagag acttcagttt
      901 aagctttttt atatcgatat tatctataag gataactccc tcctgagggt cataaaacct
      961 cataagcaga tttactatgg tggtttttcc ggcaccgctc ctgccaacca gggcgaccac
     1021 ctctccagga tagatatgaa aagacactcc ttttattacg ggttctccct ctttatacca
     1081 gaatgttaca tccctgaact ctatctcccc tcttataggc tctacaataa ttgcattggg
     1141 gtcttctact atagatggct ctacctggaa gatttcaaat attcttccta tagcccctgc
     1201 actttgactt atagtattaa agcttcggga gatcgaatat atcggatctg ctaccataag
     1261 cagggcggta aagaatgcca taagacttcc tgatgtcatg ttcccattca gcacctgaat
     1321 ccccccaaac cataataccc ctagaagccc gatagtttgg agaagagtta ctattggagt
     1381 ctgagtggct ataatttttg cactttctaa tgaaaccttg aggttctctt tacttacctc
     1441 ctgaaactca gatatctctt tatcttccat accgaaggat tttacaagat gcataccttt
     1501 tatacgctct tccagtaact tgaccagttc ggcaaatctc tgctgaacct gacgagtaat
     1561 ccgtatcatc tgatttccta gaaaggatat aactgaagct ataaatggaa gcattattaa
     1621 agtaaaaaga gtcagcctcc agttcagata tatcatatag cccagagctc ccaccatagt
     1681 taaactactc agaggaatat ccataagaat tgcgttaagt gcctgaggta ttaaggttat
     1741 atcgttggaa agacgcgata ttatttcacc tgtatgccat ctctgataaa attccataga
     1801 taatttctga agatgggcgt atacttttac tctcagatca ttcataaccc tctgagttat
     1861 tattgattgc agatatatct gccagtattg tattatactc tggagtatag taattccaat
     1921 tataacaaat atcacagtat ggaggtttct cattgcaccc gtataatctt tttgtgcatt
     1981 aaaaacacca tcaaataaga atttcaatac aggagaaagt aaaagtctag caggtgcaag
     2041 cattattgtg agcagaaaaa ggacaaaaac taaaagttta tagggtttta tatatacata
     2101 taattcccta taggtctttg agatctcacc cctcactatt cattcacctc tttgcataaa
     2161 atagataaag gacatcctct atgaatcggt atctttctgc aatacctttt acccagttct
     2221 acaagaaggg catgaaattc attatacaag tatgtatcat ttggagtatt attcataacc
     2281 atactctgaa tctctccata atcttcgttc ccccttatta tacctaacct ggaaaatata
     2341 cgtctcgtat acgcatcgac tataaagaca gggtaacctg ctgcgtaaag aattatggaa
     2401 tcagcggtct cctcccccac cccttttata ctgagtaatt cactcctgag tgactctacc
     2461 ggaagatcca ggaatatatc taaagtctta tactctttta tctttttcat aacatttatc
     2521 aatctctctg tctttaccct gtaataccca gcacttttta tcagttcagg aagcagacct
     2581 tcactctcta aaatcccatc aactgacagt ataccccttt cttttaaatt atttatggct
     2641 ttctcaacat tcttccagtt cgtattctga gtaaggatag cacctaccac cacttccagt
     2701 ttactctctc caggccacca gtgttgaggt ccaaatgtat cgtacagtgt ataatatatt
     2761 ttaagaagaa gtgtttctgg agacaaaatt cctgactctc tctggcattc tacaggtctc
     2821 atctaaattg caggaccgtt ccccttttct ttctacctct ttttctaaga gacaatcccc
     2881 acatactcta tccgagatta aaataaatct gttacactta actttaaagc ctgcgattct
     2941 catcttgtta tagttacctt ttttaaatat ctgggattgt caggattaat ccccttttcc
     3001 acggccaatc tgttggcaaa tatctgaaac gcaggggcaa agagaagagg acttaatacc
     3061 tcatccacct taaaaggtaa gacaaaattc aatctctctt cctttaatct ttcaaagcta
     3121 aagctaaaga cctcagtgtt tttctcttca agaataccta taacctcttt taaatgttta
     3181 taacttggtc cagatggcac aaaaataaag ataggcattt gaggagatac taaaactaaa
     3241 ggtccatgga gaaagtctgc agaactaaag gattcagaaa atatatatga tgtttcttta
     3301 agcttcagag aaaattctat ggcagaagga taattaaatc ctctaccaat gacgatacat
     3361 cgatccataa agtgaaaacg tgttacatct atcccataag acttatctat aacttctttt
     3421 atatacagag ggatatcatt tttcaataaa ttgaggatgt ccgttctatc acttaaaatt
     3481 gcgcaaaaca gatacagatt aaatatctct gccaggtagg ttttggtggc cgggacactt
     3541 ttttcttccc ctgcccagca taagataact tcatctgaca tttctgccag aggactatta
     3601 gggtcgttgg ttatagctat acataaagcc ccatcttttt taccctgagc aataacttca
     3661 cagacatctt ctcctctacc agactgagat actccaatta caatcctatt tttcagagaa
     3721 ggaggagttt tatatatggt aaagagtgaa ggtgcagaga gagctacagt atatcccagc
     3781 atagattcta ttaaatattt acccagatta ctggcattat ctgaggtacc tctagcggaa
     3841 atataaatcc catccagata tcttttttca agataggatt taatttttag caggacatta
     3901 atattattat ttaatatctt gccaactatt tctggctctt cccttatctc tgactccata
     3961 atcgtaatat tcaatcttat tatcctcctt taggaatctt acactcctat tttatcataa
     4021 aatttcatat gatataataa taccgtcaaa actttatagc tatcgggcta tagaaatgga
     4081 ggattttaag atgttttata aaaaattgtg ggttgcagta atggttctag ctctctttac
     4141 tttatgtgta agtggagctg ctgcacagga gcttactgag gtgtacagtg atcctgataa
     4201 tggattcagc gtatcttatc ctgaaaactg gttggaggga gaattggagg gtgctctggt
     4261 atggctctat gatgaagaga cctcttctga attgattgta gttaaagagg agatacctga
     4321 aggttttaca gccaaacagt atgcggatgc ggttggagaa tggttagagc agaatctgga
     4381 ggaatattca gaagaatctt taactgaagg tactctttca ggagcaccta gttttacaag
     4441 agtgtactct ttttctctta agggagaaga ggctactgta tctataaaga cgttagaaat
     4501 ctatactgta aaggatgatt atggacttgc agttttatgc gatacaacgt ctgagaactt
     4561 tgcctcgatg gaagatctat ttaataagat ggtcgcaagc tttaaatttt tagaagagga
     4621 aaagcccaac tagcgaaaaa attggagggg gcatacttta agatctgcct cctccaattc
     4681 agaggacagt agat
//
LOCUS       DAOTKU010000070         4690 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_15885_length_4690_cov_3.709817, whole genome shotgun sequence.
ACCESSION   DAOTKU010000070 DAOTKU010000000
VERSION     DAOTKU010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4690
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15885_length_4690_cov_3.709817"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1860)
                     /locus_tag="P5310_02645"
     CDS             complement(<1..1860)
                     /locus_tag="P5310_02645"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HRU32295.1"
                     /translation="MKLDRLSQEVWERKYRWGEEKILDDNISRVAKEAAKVEETEEKR
                     REWEEKFYELMKDTKFLPGGRILAGIGTSMHQTLFNCFTLPVIDDSIEGIFEAVKLNA
                     IIHSLGGGTGANFSTIRPEGAEVKTKGGIASGPVSFMTVFDAETKTITTGNARKGANM
                     GILNIDHPDIEKFITAKHTPGVLTQFNISVGITDEFISAVINDRDWELKFNGKVYRVV
                     KARDLWDLIVRSAYDYAEPGIINLSTINRLNNLYYVENIQSTNPCGEQPLPPAGCCLL
                     GSFNLTQFVMEPFTENARFNLESLNKYVPVAVRLLDNIIEISEYPFPAYEEEEKTKRR
                     MGIGIMGLGSTLAMLGLKYSSVEGRHQAEVIVKAIRDSAYQTSAILAKEKGPFKHFDR
                     DKYLEGEFIKRLPGEIRELIKENGIHNSHLISIAPTGTLSVVANNISSGIEPIFSLSY
                     KRRVKMSSDSIQGDSFEEYEVMDYAYKLYRELKGDGPIPSYFETAYDIKPEDHLEMLK
                     TVSFYVDSSVSKTINVPRDYPIESFFNLFLSAMQGGVKGLTVYREGSREGILIREEEK
                     KPARSGKPERPYKLNGTTYKIKTPRASYYITLNEIEEEGKRRPFEIFINTKDAS"
     gene            2293..4182
                     /locus_tag="P5310_02650"
     CDS             2293..4182
                     /locus_tag="P5310_02650"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRU32296.1"
                     /translation="MKKFIVGLLVLSLVISIFVSFGSIAFAQKKYNEAPMLADLVKQG
                     KLPSVEKRLPEKPFVVGSGVLIPKKDLPDWKVGKYGGTIRSVHSVADWAPDVFVMTNE
                     PLLSAPGIGTQGIRGNIVESFDVKENRIFTFKLRKGLKWSDGQPVTTEDVRFTYEDVL
                     LNEKLTPVFPARFRVGYSLKGEPMKLEILDEYTFRATFPKPYGGFLRNLTIESWVGYT
                     ELLKPAHYLKQFHIKYTSMEKLKPLLEKEGFKDEWWQLFHLKDFTNWEQTQAKSIGFP
                     VLTPWILKKASGGVYEWERNPYYFKVDTAGNQLPYIDKIVSTQVQDVEMANMRVLTGD
                     VDFLRESTALVKIPLYKQNEEKGSFKTVLLDMHVDSSALFINLTYNDPVYRKIVGDVR
                     FRRAVSQALNRKEIIDSVYYGYASLPETVPSAYSVAEANKLLDQVGLTKRDAEGFRLG
                     PDGKTFTMLIECGAEAPDLLPVGELIVEHLKAVGIKATLKRIDPQLRGQRTSSNEMQA
                     FIMWCHDQGWNNGWTSGIIGSSAPLWSRWYTTDGKEGEEPPAWVKKAYDIDKRRWESV
                     TGSEEYNKLWREGLAWHKQYLPLITIVEKVKYPMIVSARMGNVPRSGFAIAANFSAEQ
                     FFYEK"
     gene            4256..>4690
                     /locus_tag="P5310_02655"
     CDS             4256..>4690
                     /locus_tag="P5310_02655"
                     /inference="COORDINATES: protein motif:HMM:NF040248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRU32297.1"
                     /translation="MLGYIAYRLAIAIPLLLVLSVVSFVIIQLPPGDYLTTYINNLKL
                     SGIQLNEDEVVRLKQLYGLDKPVYMQYFIWIGNFIKGDLGRSFQWNRPVNEILAERVP
                     MTVLISVLTTLFAWIIAIPIGIYSALRQYSVFDYTFTFIGYIG"
BASE COUNT         1371 a          868 c          947 g         1504 t
ORIGIN      
        1 cgatgcatct tttgtattta taaatatctc aaagggtctc cgttttccct cttcttctat
       61 ctcatttaga gttatatagt atgacgccct tggtgttttt atcttatatg tggttccatt
      121 cagcttatag ggtctctctg gttttcctga tctggcaggc tttttttctt cttctcttat
      181 taatattcct tcccgtgaac cttctctgta tacagtaagt ccttttactc ctccctgcat
      241 tgctgagagg aaaaggttaa agaaggattc tatggggtaa tccctcggaa catttattgt
      301 cttggagacg gaagagtcta cataaaagct gacagtcttt aacatttcaa gatgatcttc
      361 aggttttata tcataagctg tctcaaagta agatggtatc ggtccatccc ctttaagctc
      421 tcgatataat ttatatgcat aatccataac ttcgtactct tcaaaagagt ctccctgtat
      481 agagtcagaa ctcatcttga ctctcctttt atatgatagg ctgaatattg gttctattcc
      541 actggaaata ttatttgcaa ccacagagag ggttcctgtt ggagctatac ttataaggtg
      601 agaattatga atcccgtttt ctttgatcaa ctctcttatc tctccgggta accgctttat
      661 aaattcacct tctaaatact tatctctatc gaaatgctta aatggaccct tctctttagc
      721 aaggattgcc gatgtttgat atgcagagtc tcttatagct ttgactataa cttctgcctg
      781 atgtctccct tctacacttg agtattttag acccagcatt gccagggtag agcctaatcc
      841 cataattcca attcccatcc tgcgttttgt cttttcttcc tcttcgtatg ccgggaaggg
      901 atactcactt atttctatga tattatccag cagtcttact gccacaggta catatttatt
      961 cagactttct aagttaaatc gtgcattctc tgtaaatggt tccattacaa actgggtaag
     1021 attaaaacta ccgagcaagc aacatccagc gggaggaagt ggctgttctc cacaggggtt
     1081 agtcgattgt atattttcta cataatagag attgttcaac ctatttatag ttgaaagatt
     1141 aattattcct ggctctgcat aatcgtaagc acttcttact ataagatccc ataagtctct
     1201 tgcttttact accctgtaaa ctttgccatt aaattttagt tcccagtctc tgtcatttat
     1261 taccgcagat ataaattcat ctgttatgcc gacacttata ttgaattgtg taagtacccc
     1321 aggggtatgt ttggcagtaa taaatttttc tatatctgga tgatcaatgt ttaatatacc
     1381 catattagcc ccttttcggg catttccagt agttatagtc ttggtctctg catcaaaaac
     1441 agtcataaaa gacaccggcc cactggcaat tccccccttt gtttttacct ctgccccttc
     1501 tggtcttatt gtggaaaagt tggctcctgt tcctccccct aaagagtgta taatagcatt
     1561 taattttaca gcctcaaaaa tgccctctat agaatcatct ataactggca gggtaaagca
     1621 attgaataac gtttgatgca tggatgttcc aataccagcc aggatacgtc cacctggcag
     1681 aaactttgta tctttcataa gctcgtaaaa tttctcttcc cactctcttc tcttctcctc
     1741 agtctcttca accttagcag cttccttcgc tacacgactt atattatcat ccaggatttt
     1801 ctcctctccc catctatact ttctttccca gacttcctga gataatctat ccagtttcat
     1861 tcttccctcc aaaatgtttt tacaaagata ttatattaca acttatagat atccaactgc
     1921 ataaagtagg gtgtatttac atatacgaac ttgacaagat tttttataga tatatccttg
     1981 atattttcct gtggacattt gtacctctat tgacaatagt tgtttaaatt cctataatca
     2041 tccctgtagg agatgatatg aaatttgagg attttcggag agcttttgac agaaatatag
     2101 aggatgagat ataaagaaat ttaaagggtg tgggcaggaa caactggagt tctcgaaaat
     2161 cttgtgagga ggtgatgtat ccttagggta taagatttag atgtttaaaa gatttggaag
     2221 taacccagtc aagatatccc aagagggaag caggaaaatt taatctaaaa ctaaaaagag
     2281 gaggtttcga tagtgaagaa atttatagtg ggattacttg tattaagttt agtaataagt
     2341 atatttgtga gttttggctc tatcgctttt gctcaaaaga aatataacga agcaccaatg
     2401 ttggccgatc ttgtaaagca aggtaagctt ccatctgtag agaagagatt acctgaaaag
     2461 ccatttgttg taggttctgg agttttaatc ccaaaaaagg accttccaga ttggaaggta
     2521 ggcaagtatg gtggaacaat aaggagtgtc cattcagtgg cagactgggc tccagatgta
     2581 tttgttatga ccaatgagcc attattaagc gctcctggca taggaacgca gggtataaga
     2641 ggtaacatag ttgagagctt tgatgtcaaa gaaaatagaa tctttacttt taaactcaga
     2701 aaaggattaa agtggtcgga tgggcaacca gtaactacag aagatgtaag gttcacctat
     2761 gaggatgttc ttctaaacga gaagcttacg ccagtattcc ctgcaagatt cagggtcggc
     2821 tacagcttaa aaggtgaacc aatgaagtta gagatactgg atgaatacac cttcagggct
     2881 accttcccga aaccttacgg tgggttcctg agaaatctta ccatagaaag ctgggtagga
     2941 tatactgaac ttctgaagcc agctcattat ttaaagcagt tccacattaa atatacctct
     3001 atggagaagc tgaaaccatt gttagagaag gaaggattta aggatgaatg gtggcaattg
     3061 tttcatctaa aagattttac taactgggaa caaactcagg caaagagtat aggatttcca
     3121 gttcttactc catggatatt gaagaaagct tctggtgggg tctatgagtg ggagaggaat
     3181 ccatactatt tcaaggtgga tactgcaggt aatcaattgc catatataga caagatagtc
     3241 tccacccagg ttcaggatgt agaaatggcc aatatgagag ttcttactgg tgatgtggac
     3301 ttcttaagag agagtacagc tctggtgaag attcctctat acaagcagaa cgaggaaaag
     3361 ggaagcttta agactgttct gcttgacatg catgtagatt caagtgcact tttcatcaat
     3421 cttacatata atgatccagt ctatcgtaag atagtaggtg acgtgagatt tagaagagct
     3481 gtaagtcagg ctctgaaccg taaagagatt atagacagtg tctattatgg ttatgcttct
     3541 ctcccggaaa ccgtaccaag tgcttatagt gtagctgagg ctaataaact attagaccag
     3601 gtagggttaa ccaagagaga tgcagaaggt ttcaggttag gacctgacgg taaaacattt
     3661 accatgctaa ttgaatgtgg agctgaagca cctgacctcc ttcctgtagg agaacttatt
     3721 gtagaacatc ttaaggcagt aggtattaag gctacattaa agaggataga tccacaactt
     3781 agaggtcaac gtacttcaag taatgagatg caggctttta taatgtggtg ccatgatcag
     3841 ggttggaata atggctggac atctgggatt attggttcaa gcgctccatt gtggtccaga
     3901 tggtatacca cagacggtaa agaaggggaa gaaccacctg catgggtaaa gaaagcctat
     3961 gatatagata agagaagatg ggagagtgtt actggctctg aagagtataa taagctctgg
     4021 agagaagggc ttgcctggca caagcagtac ttgcctctta tcaccatagt agaaaaggtg
     4081 aaatatccga tgatagtttc tgccagaatg ggtaatgtcc ctcgttctgg atttgctatt
     4141 gcagcgaact tcagtgcaga acagttcttc tatgagaaat agataggcta agtgggctat
     4201 cagggaattc cctgatagcc ctatatatca tcttttaatt ataggaggaa ttagaatgtt
     4261 aggatatata gcctatagat tagctatagc aattccactt cttttagttc tatctgtagt
     4321 gtcttttgtt ataattcaac ttccgcctgg agattatctc actacatata taaacaatct
     4381 gaaactctca ggtattcagt taaatgaaga tgaggttgtt agattaaaac agttatacgg
     4441 gttggataaa cctgtttata tgcagtattt tatatggata gggaacttta ttaaaggtga
     4501 tctgggcagg tcttttcagt ggaatcgtcc agtcaatgaa atcttggctg aacgtgttcc
     4561 tatgactgta ttaatatcag tattaacaac cttattcgcc tggattattg caattcctat
     4621 cgggatatat tctgccctga gacagtactc tgtttttgat tatacattta cattcatagg
     4681 ttatataggt
//
LOCUS       DAOTKU010000071         4670 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_15950_length_4670_cov_3.157313, whole genome shotgun sequence.
ACCESSION   DAOTKU010000071 DAOTKU010000000
VERSION     DAOTKU010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4670
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15950_length_4670_cov_3.157313"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="P5310_02660"
     CDS             <1..288
                     /locus_tag="P5310_02660"
                     /inference="COORDINATES: protein motif:HMM:NF014683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HRU32298.1"
                     /translation="GTFLGGSDTTSNALFGALQTEAAKSIGVSPVWLASANATGATGG
                     KLISIQNLTLAVSGINKKGEEGKLMRVVFPYFLFYLLIMGVIIFIGARVFG"
     gene            complement(295..1611)
                     /locus_tag="P5310_02665"
     CDS             complement(295..1611)
                     /locus_tag="P5310_02665"
                     /inference="COORDINATES: protein motif:HMM:NF012246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RuBisCO large subunit C-terminal-like
                     domain-containing protein"
                     /protein_id="HRU32299.1"
                     /translation="MEAYIPYELKGKELLEVINLGSKFSKEESIGLKFYIKTTGNLVK
                     VAQDIARDETTGPWVGQGEPTQLFIRSQADVDRIERYGEKEGVIYIRTPLSNISSETD
                     PLYQFIMLAVGGPVLEFVYYQEVALLDFSFPRNFLERFPGPKFGINGVRKLLNLKDGE
                     PIIGTIVKPCAGLTPREVAEKCYQSALGGVMFIKDDEKMLGPDYCPLEEKVKLVSQAL
                     KRAEEETGRKCLYAPHIVARADKIKDVAKRAIEWGATALMFNAVLGHTYEALSILAED
                     KEIDVPLYAHSGGRSGLSTGPRRIDDTVIAKLLRYSGADFFQHGVFGMKEMHVASLDD
                     GLLHNLVRVMREDVGVRDTIPVAAGGLGVTNAGINLKEHKDERFGYSVALLAGSNILK
                     HPDGPKAGATAMHQAVQAYYEKGITEKGQLKEYAIKRGWEELLKIL"
     gene            1791..>4670
                     /locus_tag="P5310_02670"
     CDS             1791..>4670
                     /locus_tag="P5310_02670"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32300.1"
                     /translation="MSKNFTDSIKEIWVVPGFHSDVVWLEDQRDYATVLLGDADQNIQ
                     VCRADPHYGVFLHELTYLKPYFDTYPWNREFLKSLIRDGRVGTGGSHSQPAETLIGPE
                     GLIRNIIYGRLFHEGVLHSKPEIYMPWDVFGHSAQLGQILRKSRFTGCIWSKDIRGAE
                     PVFWHLSLDGTRLLFKRMNYGIRGSGFEEEFIKYLEDHSYEIASLGLTSDVRLDAGDF
                     KPPSAWLVGRCAELKSRNPRIIISGIGHEKWFRKTLHEIEERKLLIPTTARDFEWHHQ
                     GTGVSRIEFKLANRKGENLLLDAEKFATIANYLGAEYPDKALDKAWRQLLFNQHHDAI
                     TGPLCDRSYIDLMLGYREALEIGREVLDNSLDYIGKYIDTSGVRSENAVPFAVFNSLN
                     WERTDVCRIEVSFPQGVDNFKVIDHTGKEVCFEILQVNRNGNGKIIKAEILILAEDVP
                     SLGYKVFYAVPMEGSPAEIPSSEGNTIENDFFRITVDPTLGGGIVSLYDKLAGKELIP
                     SNSKGSGIPGNELVALSEDPARHEPAWEIFTTGEKVFSRDYPASVNVKSGPLISKLII
                     KGDMKDCQRYQEIILYRGIPRVEFLTRLEGYSGAHDLYVITFPADLNGLEPVFEERFG
                     ATVKRKSKGYLDFRTWQWRNYSECGARRCYQWFGLGQNGMIECGDKGDEGYTQVVIGM
                     VGLVTGHNPKVFTLSERIQESLIRRGIPVTPFFDDLDIERRSSLPVQDSCMPTENLNE
                     DLPWGTSFRVALDLDGDNSYFQKVFETIPDDKKAYYREQLKTKGFACIISLDHDIPEG
                     WTPLPVLLVGAKDIESLEKAIDSILCNIPQGLIKLPADSNATGISSTVDDYGLILLNT
                     GNVLGSVEHDNTLVLFLMHTAAWGSTPWGKDRLPYMFVPEWKTHQFLYALYPHQGDWR
                     SARAFRAGYEYNNPLIGRQLELHKGKLLPEEAFVKFEGDNLVMTAM"
BASE COUNT         1299 a         1048 c         1015 g         1308 t
ORIGIN      
        1 ggaacatttc tcggtggaag tgataccaca tccaacgcac tctttggggc tctccagaca
       61 gaggcagcta aaagtatagg cgtttctccc gtatggctgg ccagtgccaa tgccacaggg
      121 gctaccggag ggaaactaat atccattcag aatctcaccc tggctgtttc aggtataaac
      181 aaaaaaggag aagaggggaa acttatgagg gtagtctttc catatttcct cttttatctc
      241 ttaattatgg gagtaatcat atttatagga gccagggtat ttggttaagt ctttctataa
      301 tatcttcagt aattcttccc accctctctt aatggcatac tctttcaatt gccccttttc
      361 agttatcccc ttttcatagt aagcctgaac tgcctgatgc atagctgtcg ctccagcttt
      421 gggaccatct ggatgcttca gaatatttga accagctaaa agagccacac tataaccaaa
      481 tctttcgtct ttatgctctt taagattaat tccagcattg gttacaccca gaccacctgc
      541 agctacaggt atcgtgtctc ttacgcccac atcttctctc ataactctga caaggttatg
      601 gaggagtccg tcatccagag aggcaacatg catctctttc ataccaaaaa ccccatgctg
      661 gaagaaatct gctccactgt accgcagtag ctttgctatc acagtatcgt ctatccttct
      721 tggtccggta gaaagccctg atcttccacc agaatgggca taaagaggaa catctatctc
      781 cttatcctca gccagaatac tgagagcttc ataggtatga cccaggaccg cattaaacat
      841 taatgcagtg gctccccatt ctatagccct ctttgccaca tctttaatct tgtcagccct
      901 agcaacaata tgtggtgcat agagacattt tctaccggtt tcctcctctg ccctctttaa
      961 agcctgagag acaagtttta ccttttcttc caagggacaa tagtctggac caagcatctt
     1021 ttcatcatcc tttataaaca tcacccctcc taaagcagat tgataacatt tttccgctac
     1081 ttctctgggg gtaagaccag cacagggttt cacaattgtg cctattatag gttctccatc
     1141 cttaagattt aacagtttcc tcaccccatt tataccaaac tttggtccgg ggaatctttc
     1201 caggaaattt ctgggaaaag aaaaatccag aagcgcgacc tcctgataat aaacaaattc
     1261 aagcacgggt ccccctaccg caagcataat aaactggtat agagggtctg tctcactgga
     1321 aatattggaa aggggtgttc ttatataaat aaccccttct ttttcaccgt atctctctat
     1381 cctatcaaca tctgcctgag atcggataaa taattgggta ggttctccct gccctaccca
     1441 gggtccggtt gtttcgtctc tggctatatc ctgcgctacc ttgaccagat ttccggtagt
     1501 ctttatataa aatttaagcc ctatagactc ctctttgcta aattttgaac caaggtttat
     1561 aacctccaga agctctttgc ccttcaactc atatggtata tatgcttcca tatccttatc
     1621 ctcctatcag agcggattaa ttttgactat attctaacac agcaataaaa atttacaaga
     1681 atcagtatat atttatgttt gaatttatgt aaaaatacaa tgtgtataat gtaaaactgt
     1741 tgacatatta aaatagagga gtagaatcta ttatcaggag ggcagatatt atgagtaaaa
     1801 attttactga ttctattaaa gagatctggg tggtaccggg attccactct gacgtagttt
     1861 ggcttgaaga ccagcgtgac tatgcgacag ttttacttgg agacgctgat cagaacattc
     1921 aggtctgcag ggctgatcca cattatggag tctttctcca tgaactgaca tacctgaagc
     1981 catattttga tacatatccc tggaacaggg aatttctcaa atcactcatc cgtgacggtc
     2041 gggttggaac gggtggttct catagccagc ccgccgaaac tcttatcgga cctgaaggtc
     2101 tcatccggaa tatcatctat ggtcgacttt tccatgaagg ggtgcttcat tcgaaacctg
     2161 agatttatat gccctgggat gtgtttggac attcagcaca actcggacaa atcctgagga
     2221 aatcaagatt tactggctgt atatggtcaa aggatataag gggagctgaa cctgtattct
     2281 ggcacctatc cctcgacgga actcgtcttc tcttcaaaag aatgaattac gggatacggg
     2341 gaagtggttt tgaagaggaa tttatcaagt atcttgagga ccattcttat gaaatagcct
     2401 cattagggct tacatctgat gtacgcctcg atgctggaga tttcaaacct ccatctgcat
     2461 ggcttgtggg aagatgtgcc gagcttaaaa gccgaaatcc aaggattatc atctcaggga
     2521 tagggcatga aaagtggttc agaaaaactc ttcatgagat agaagaaagg aaacttttga
     2581 ttcccacaac cgccagggat ttcgaatggc accaccaggg tacaggtgtt tcacggatag
     2641 agtttaagct tgccaaccga aagggtgaaa acctcttgct ggatgcagag aagttcgcca
     2701 ccatcgcaaa ttatctgggt gctgaatatc cagataaagc tctggacaaa gcctggcgcc
     2761 aactactttt taaccagcat cacgatgcaa taacaggtcc tttatgtgac cgttcttaca
     2821 tagacctgat gctcggatac cgtgaggcac tggagattgg tcgagaagtt ctggataact
     2881 cccttgatta tataggaaag tatatagaca cctctggcgt tcggtcagag aatgctgtcc
     2941 cctttgcagt ctttaactca cttaactggg agcgcacgga tgtatgccgt attgaagtca
     3001 gttttccgca aggagtggat aatttcaagg taatagacca cactgggaaa gaggtatgct
     3061 ttgagatact ccaggtgaat cgaaatggaa atggcaaaat cataaaggct gaaatactca
     3121 tccttgccga ggatgtgcca tctttagggt ataaagtctt ttatgcggta cctatggagg
     3181 gttcccctgc tgagatacca tcctctgagg gaaataccat tgagaatgat ttcttccgaa
     3241 tcacagtcga tcctacactt ggaggtggta tagtaagtct ttatgataaa ctcgccggga
     3301 aggaacttat tccatccaat tctaaaggct caggtatccc tggaaacgag cttgtagctc
     3361 tctcagaaga tcctgcacgt catgaacctg cctgggaaat cttcaccaca ggtgaaaagg
     3421 tcttctcaag agattaccct gccagtgtaa atgtcaagtc aggtcccctc atatcgaagc
     3481 tcatcataaa aggtgatatg aaagactgcc agcgttacca ggagataatt ctctatagag
     3541 gaataccaag ggtagaattc ttgaccagac ttgagggtta ttcaggagcc catgaccttt
     3601 acgtgataac atttccagca gatcttaacg gacttgaacc ggtatttgaa gagcgtttcg
     3661 gagctacagt gaaacgtaag agcaagggtt atctcgattt ccgcacctgg cagtggagaa
     3721 actattcaga atgcggggca aggcgatgct accagtggtt tggtctcggg caaaacggca
     3781 tgatcgaatg tggagataaa ggcgacgaag ggtatacaca ggttgttatc ggtatggtag
     3841 gactcgttac cggtcataac ccgaaggttt ttactctttc agagaggatt caggagtcac
     3901 tgatccgccg ggggattcca gttacaccat tctttgatga cctagatata gaacgcaggt
     3961 cttcccttcc cgttcaggat tcatgtatgc ccactgagaa tctaaatgag gaccttccct
     4021 ggggaacttc attcagggtt gctcttgacc tagacggaga taacagctat ttccagaagg
     4081 tatttgaaac tatacctgat gataaaaaag cttattatcg ggagcagctg aagactaaag
     4141 gttttgcatg tataatttct ctagaccatg acatcccaga aggctggact ccactaccgg
     4201 tcttacttgt aggagcaaag gatatagaaa gcctggagaa agccattgat agcatcctct
     4261 gtaacattcc gcagggactc atcaagctcc cagctgattc taatgccaca ggtatctctt
     4321 cgacagttga tgattatgga ttaatccttc ttaataccgg gaatgtactg ggttcggtag
     4381 agcatgataa tacactggtc ctgttcttga tgcacacggc tgcatggggg agtactccat
     4441 ggggcaaaga ccgtctcccc tatatgttcg tgccagaatg gaagacccat caatttcttt
     4501 acgccctcta tcctcatcag ggcgactggc gttcagccag ggctttccgg gctggatacg
     4561 agtataataa cccgcttatc ggaagacagt tagagcttca taaaggtaag cttcttccag
     4621 aagaggcatt cgttaagttc gaaggagata accttgttat gactgctatg
//
LOCUS       DAOTKU010000072         4664 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_15972_length_4664_cov_3.727490, whole genome shotgun sequence.
ACCESSION   DAOTKU010000072 DAOTKU010000000
VERSION     DAOTKU010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4664
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15972_length_4664_cov_3.727490"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(194..1336)
                     /locus_tag="P5310_02675"
     CDS             complement(194..1336)
                     /locus_tag="P5310_02675"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRU32301.1"
                     /translation="MVGKERVSLVLEHKIPDRVPWGEYAIDYDTVEKILGHETYLRAK
                     AKTQIAFWEGRRDEVAQSWREDLIELYRKLDFIDIINLAAMCTAILPPKDYIPEKPKR
                     IDENTWEFEEGRIYKYSSVTGDITLVYDPQKWTREFKLEDFKYEEPLPPDESRFEVLD
                     VVIEEFKDEKFILGPDGGEVSLLLLGDMERGLIEYIENPEVVKRAIDVALYRANKLDK
                     FYIREGQDGVMWGTDFASNKGPFISPRMFREFCLPAIKERVRNVKGFGMKVMKHACGN
                     NTQLMDIFVEAGYDAYQSIQETAGMDLKWLKDKYGDKLVLWGGVNVENLVSGTPEDVK
                     RDVKKAMEILKPDGGYIFGTSHTIAVGTKYDNFMAMVDEYLRLCDY"
     gene            complement(1349..2722)
                     /locus_tag="P5310_02680"
     CDS             complement(1349..2722)
                     /locus_tag="P5310_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015908389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HRU32302.1"
                     /translation="MKQDIKIAYIGGGSRGWAWKLMTDLALEKDLSGTVRLYDIDYEA
                     AKNNEIIGNKLSVKPEVVGRWNYMAVKTLDEALYGADLVVISILPGTFDEMYSDVHAP
                     EKYGIYQSVGDTTGPGGLIRGLRTVPMYVEFAEGIKKNCPDAWVINYTNPMAICLKSL
                     YEVFPKIKAFGCCHEVFGTQELLVDVVKHYIGEDKEITRRDIKVNVLGINHFTWFDKA
                     SYNSIDVFPLYRQFIDEYYEEGYEREKGRWERDYFASANRVKFDLFKRFELIGAAGDR
                     HLAEFIPYIYLRDVETARRWKFSLTPVEWRIKNRERLIELSKEFVSGERDVPLHPSGE
                     EGVMQMKSIFGLDTFVTNVNLPNRGQIPNLPACAIVETNAVFSRDDVRPVFAGELPSD
                     LASIMIRHISNQELIVRAALEKDINLARRAFLNDPAVERLSADDANKLFNEMIDNTKE
                     YLKYLGL"
     gene            complement(2742..3632)
                     /gene="garR"
                     /locus_tag="P5310_02685"
     CDS             complement(2742..3632)
                     /gene="garR"
                     /locus_tag="P5310_02685"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017490885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HRU32303.1"
                     /translation="MKIGFIGLGIMGKPMAKNLMRAGYSLIVYDINKEPVKELMSLGA
                     EGGSSPKDVAERTDIIITMLPDSPQVKDVILGENGVLEGARRGAIIIDMSSIAPAVSK
                     EVAIKAEEKGVEFLDAPVSGGEPKAIDGTLAIMVGGKKEVLDRCYDILNKMGSSITLC
                     GEAGAGNITKLANQIIVALNIAAMSEALVLGTKAGVDPEIIYKAIRGGLAGSTVLDAK
                     VPKVLARDFSPGFKIDFHIKDLKNAISTAHEVGVPLPLTSQIMEILQALKVDGKGLSD
                     HSAVIQFYEELAKVEVKRKE"
     gene            complement(3643..4581)
                     /locus_tag="P5310_02690"
     CDS             complement(3643..4581)
                     /locus_tag="P5310_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015263744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HRU32304.1"
                     /translation="MEMASLREAYGKALVELGAQNKNIVALDADLSKSTRTALFQEAY
                     PERFFEISIAEQNMIGVAVGLALTGKIPFVNSFAVFATGRTYDQIRQSACIANINIKI
                     CGSSSGLSDFGDGATHQSLEDVALMRVLPNMTVLVPVDAVETKKMVEFAVRHNGPVYL
                     RINRNDLPVYTDESTEFELGKLYQMRDGEDAVVFANGVMVSKAMEAAEILEKEGTGVR
                     VVNVSTVKPLKKEEVLKYIKGARGIVTAEEHSIIGGLGSAIVELLRDVDHPPVEFIGV
                     KDRFGTSASNYEELLEYFGLTSKSITLTIKDLLDRC"
BASE COUNT         1224 a         1268 c          668 g         1504 t
ORIGIN      
        1 aatttctata tctcttctcc ctgcacatca ccctttctcg aattataact ttcataacct
       61 aacccccttt actagcactc atctatcaat gtacactacc ttccccttta atcccagata
      121 catcataact tatctatatt ttatcccact aaatatatat gtcaagggac agtctgctga
      181 gcttccccta tcatcagtaa tcacaaagcc tcagatactc atcaaccata gccataaagt
      241 tgtcatattt tgtcccaact gcaatggtat gtgatgtccc aaagatatat ccaccatcag
      301 gttttaatat ctccatcgct ttttttacat ctcttttcac atcctctggt gtcccactta
      361 ccagattctc tacgtttact ccgccccaga gtacaagttt atctccgtat ttatctttta
      421 gccatttcag atccatcccg gcagtttcct gtatggattg ataggcatcg tatcctgcct
      481 ccacaaaaat gtccataagc tgagtattgt tgccacaggc gtgtttcatt accttcatcc
      541 caaatccttt tacattcctg acacgttcct ttatcgcagg taaacaaaat tctctgaaca
      601 tcctggggga tataaatggt cctttatttg acgcaaagtc cgtaccccac ataaccccat
      661 cctgtccttc tcttatataa aatttatcaa gtttatttgc cctatataat gcaacatcaa
      721 tagctctttt taccacctct ggattttcta tatattctat aagccctctt tccatatcac
      781 ccagtaataa taggcttacc tccccaccat caggtccaag gataaacttt tcatctttga
      841 attcttctat aaccacatct aagacttcaa agcgtgattc atctggaggc agaggttctt
      901 catacttaaa atcttcaagt ttgaattctc ttgtccattt ttgagggtca tataccagtg
      961 ttatatctcc tgttactgag gaatatttat agattcttcc ctcttcaaat tcccaggtat
     1021 tttcgtctat cctcttcggt ttctctggaa tatagtcctt agggggaagt atagcggtgc
     1081 acatagcagc caggtttatt atatctataa aatctaactt cctgtaaagt tcaatcaggt
     1141 cttccctcca gctctgggcg acctcatctc ttctcccttc ccagaatgct atctgagtct
     1201 tagccttagc tctaagatag gtctcatgtc ccagaatctt ctccaccgtg tcataatcta
     1261 tcgcatactc cccccaggga accctgtcag gtatcttatg ctccaagaca agtgagactc
     1321 tttcttttcc aaccataacc gattgttcct ataaacccag atacttcaga tactctttgg
     1381 tattatctat catctcatta aagagcttat tcgcatcgtc agcacttaac ctctctacgg
     1441 caggatcatt aagaaatgcc cttctggcaa ggttaatatc cttttcaagg gctgctctta
     1501 caatcagctc ctgattgctt atatgcctta tcattatgct ggctaaatct gatggtaatt
     1561 ctccagcaaa gacaggtcta acatcatccc tactgaaaac agcattagtt tctactatcg
     1621 cacaagctgg tagattggga atctgtcccc tgtttggcag gtttacattt gtgacaaaag
     1681 tatccagacc aaaaatggat ttcatctgca ttacaccctc ttctccagaa gggtgtaatg
     1741 gaacatctct ctccccagag acaaactctt tactcagttc aatcaatctc tccctatttt
     1801 ttatcctcca ctctacaggg gtaaggctga acttccatct ccttgcagtc tccacatctc
     1861 tgaggtatat atatgggatg aattctgcca gatgtctatc accagccgca cctataagtt
     1921 caaatctctt aaacaggtcg aatttaactc tattcgcaga ggcaaaataa tctctctccc
     1981 agcgtccctt ttctctctca tatccttctt cataatactc atctataaat tgtctataca
     2041 atggaaatac atcaattgaa ttataagaag ccttatcaaa ccatgtaaaa tggttaatcc
     2101 ccaatacatt aactttaatg tctcttctcg ttatctcttt atcttcacct atataatgct
     2161 ttaccacatc aaccagcagt tcctgagttc caaatacctc atgacaacat ccaaacgcct
     2221 ttatcttggg gaagacctca tagagcgatt taaggcatat agccatcgga ttggtatagt
     2281 taatcaccca tgcatctgga cagttcttct ttataccttc ggcaaattcg acatacatag
     2341 ggactgtcct taaacctctt ataagtcctc caggacctgt agtatctccc accgattgat
     2401 atatcccata cttctcaggg gcatgtacat cagagtacat ttcatcgaat gtccctggaa
     2461 ggattgatat aactacgagg tctgctccat aaagagcttc atcaagtgtc tttacagcca
     2521 tataattcca tcttcccacc acttcaggtt taactgagag tttattacct ataatttcat
     2581 tattcttagc tgcctcatag tctatatcgt aaagtctaac tgtcccgctt aaatctttct
     2641 caagagcgag atctgtcatc aacttccacg cccaacccct tgaaccccca cctatgtatg
     2701 caatttttat atcctgtttc accttatccc tccgatatct attattcttt cctctttacc
     2761 tcaaccttcg ccagttcctc ataaaactgt ataactgcac tgtggtcact caaccctttc
     2821 ccgtcaactt ttaacgcctg cagtatctcc ataatctggc tcgtaagtgg tagtggaact
     2881 ccaacttcgt gggcagtaga tattgcattc ttcaggtctt ttatatgaaa atctatctta
     2941 aaaccagggc tgaagtccct cgcaagtacc tttggaacct tggcatccag tacagtactc
     3001 cccgctaaac ctccccttat cgctttgtag attatctccg ggtccacccc agcctttgta
     3061 cccaaaacca gcgcctctga catcgccgct atatttaacg caactattat ctgattcgcc
     3121 agctttgtta tattcccagc tccagcttct ccacagagtg taatagaact gcccatctta
     3181 ttgagaatat cgtaacatct atccagaact tccttcttcc cccctaccat tatggcaagt
     3241 gtcccgtcta tagccttcgg ctcccctcca cttacaggtg catcgagaaa ttccacacct
     3301 ttctcttcgg ctttaattgc tacctctttt gaaacggcag gagctatcga actcatatca
     3361 attattatcg ctcccctcct ggctccttcc aggaccccat tctccccaag aataacatct
     3421 ttcacctgag gtgaatccgg tagcatagtt attattatat ctgtcctctc agctacatcc
     3481 tttggggaag accctccttc tgcaccaaga ctcatcagtt cctttacggg ttccttattt
     3541 atatcatata caataagact atagccagct ctcataaggt tcttagccat aggctttccc
     3601 attataccaa gtcctataaa tcctatcttc atagattacc tcctagcatc tgtccagtaa
     3661 gtcttttata gttaaagtga tagacttcga tgttaaccca aaatactcta ataactcctc
     3721 atagttactg gcagaagttc caaacctatc tttaacacct ataaactcca caggtggatg
     3781 gtctacatct ctaagcaact ctactatagc gctccctaat cctcctatta tactgtgttc
     3841 ctcagctgtt actatccctc tcgcgccttt tatatatttt aacacctctt ccttcttcaa
     3901 gggtttaaca gtactcacat taacgaccct aacacctgta ccctcttttt ccaggatttc
     3961 tgccgcttcc atagcctttg ataccatcac tccattggca aataccactg catcttcgcc
     4021 atctcgcatc tggtaaagtt ttcccagctc aaattcagtc gattcgtccg tatatacagg
     4081 aaggtcgttt ctgtttatcc tcagataaac aggtccgtta tgtctcacag caaattccac
     4141 catcttcttg gtctcaacag catctacagg aacaaggacc gtcatatttg gcaacaccct
     4201 catcaatgcc acatcttcca gagactggtg ggtggctcca tctccaaagt cagacagtcc
     4261 agagctggag ccacaaattt ttatatttat attggctata caggcagatt gtcttatctg
     4321 atcatacgtt ctgccagtag caaagacagc gaaagaatta acaaatggaa tcttacctgt
     4381 taaagctaaa cctaccgcta cccctatcat attctgttca gctatactta tctcaaagaa
     4441 cctttccgga taagcctcct ggaacagggc agttctggtt gatttactca ggtcagcatc
     4501 cagtgccact atatttttat tctgagctcc aagctctacc agcgcctttc cataagcttc
     4561 tcttagactt gccatttcca tatcagctcc acctcgccat cctggattcc agctctcttt
     4621 tagcctgaat atactgttct tcagtcaatg caatattgtg gaat
//
LOCUS       DAOTKU010000073         4650 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_16045_length_4650_cov_3.418281, whole genome shotgun sequence.
ACCESSION   DAOTKU010000073 DAOTKU010000000
VERSION     DAOTKU010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4650
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16045_length_4650_cov_3.418281"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="P5310_02695"
     CDS             complement(<1..648)
                     /locus_tag="P5310_02695"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRU32305.1"
                     /translation="MLIGRVALITGASRGIGRAISTKLAQNGVNLAVNGKSEGSLVVL
                     KEDLSPYGIGILLCPGDLQDPDVPSMIVERTINYLGRLDILINSAGVALAKPLSETTV
                     EEWERLMMVNARAPFLLCREAVPYLKNSDIATIINISSLVGIKGYINQGAYTASKHAL
                     MGFTKVLAQEVHQDGIRIHIISPGVIDTEMETMTESDIDPFDLMSPDEIAEIVLFL"
     gene            773..1546
                     /locus_tag="P5310_02700"
     CDS             773..1546
                     /locus_tag="P5310_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HRU32306.1"
                     /translation="MVSPFKEIKVDEVLVKIYKNRDEMGKAASLDVAERIREIARVKD
                     TINIVFAAAPSQNEFLSYLNMEKLDWNKINAFHMDEYIGLPADAPQSFASFLKERLFS
                     KKPFKNIFYINGNAVSPEEECNRLSRLLEDNPLDITCMGIGENGHIAFNDPHVADFDD
                     PKKVKIVDLDEKCRQQQVNDGCFKDIDDVPRYAITMTIPTLVSSPFIFCIVPGKQKAE
                     AVRNTLLGPVSESCPASIIRRCKNSILYLDLDSASKLEL"
     gene            complement(1636..2247)
                     /locus_tag="P5310_02705"
     CDS             complement(1636..2247)
                     /locus_tag="P5310_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32307.1"
                     /translation="MKALRSTLVVFLILIIFNVPSIAMGNEADASIVISQDALNKFLL
                     VIGETKKTDTFKILGITGEYTWVVKNPRIVLSAGKAQFLADVTVSLKTPPVNYSVPAH
                     GEVSIKYDPNGNKISIKVEKVGFDVAFNILGKKIVVGEVDISRIYQIAFSFPGPKPFE
                     SLIEVGLPDGGKKKIRLESIPVLTVEDGNIVVGSEINYIPVAE"
     gene            complement(2339..3445)
                     /locus_tag="P5310_02710"
     CDS             complement(2339..3445)
                     /locus_tag="P5310_02710"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013402197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HRU32308.1"
                     /translation="MCDTIVALRNSTSDGSVLFGKNSDREKEEPHIIIRVPAKEHRVG
                     EKVRCTYIEVEQSEITYDCILCKPSWIWGAEMGVNENGVVIGNEAVFTNQKQGPPALL
                     GMDILRLALERSKTAKEAVDNIVYLIERYGQGGKCGYTKDLRYDNSFLIADFNYAWVL
                     ETAGSYWAVEEVKDVRSISNALSIKDFKKKYENRLITRFSCGDFRQSLTAGMLEEKKG
                     RLTVEDFKAILRNHHNLNSKNILYGSMKNICMHSKSIISSETTGSLVVELINGEINIF
                     ATGSSLPCLSIYKPLWFVKNPDLFYSEDEVDAAVAYWKRQKETIEQVAEGNRDRTAYL
                     ENRDRIERELFELARSARDELEREEIIKRAWGMM"
     gene            complement(3450..4058)
                     /locus_tag="P5310_02715"
     CDS             complement(3450..4058)
                     /locus_tag="P5310_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011024997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRU32309.1"
                     /translation="MNDLIIRTGKIEDAYDFSHLILLSAPVFFPSLFGDNVLEVMENL
                     FRYPRNIFSFEHSYFIEMENKVAGMVLGYTWKEREREELYTGFLLFKYLGWDILTRVP
                     YLLRVQSILGNLVAGDYYISNIAVYPEFRCSGLGTRLLEKVEEEAGERGCKMMVLDVE
                     ADNRGAISLYEKLGYNAGERSSVVKIKGKSFEFFRMKKKIGG"
     gene            complement(4080..4349)
                     /locus_tag="P5310_02720"
     CDS             complement(4080..4349)
                     /locus_tag="P5310_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32310.1"
                     /translation="MDNIEWVWNEESKLTEEGKEKILEFIKGFEENEGGVLDILIEGV
                     GGHFTQILDSSRPEDWKFFPTEWICYRKDKEEMARKIIGTLHPDM"
BASE COUNT         1384 a         1096 c          752 g         1418 t
ORIGIN      
        1 taagaacagg actatttctg ctatctcatc cggagacata aggtcaaatg ggtctatatc
       61 tgattcggtc attgtttcca tttcagtatc tataactcca ggagaaataa tatgtatcct
      121 gataccatcc tgatgaacct cctgagccag taccttggta aatcccataa gggcatgctt
      181 cgatgctgta tacgccccct ggtttatata cccctttata ccaaccaaag aagagatatt
      241 aataatggtt gctatatccg agttcttaag atacggtaca gcctccctgc acaacagaaa
      301 tggagcacgt gcatttacca tcatcagcct ctcccactct tccactgttg tctcagagag
      361 aggttttgcc aatgctaccc ctgcactgtt tataagtatg tccaatctcc ccaaataatt
      421 tatagtcctc tccactatca tagacggtac atccggatcc tgaaggtcac caggacataa
      481 cagtatacct atcccataag gactcaggtc ttcctttaat actacaagac ttccctctga
      541 tttcccattt acagccagat taactccatt ctgagcaagt tttgttgata tagctctacc
      601 aattccccta ctggcgccgg taattaaggc taccctccca attaacatct taccctccat
      661 aatgtttaat ctctggaaaa gattataaca catattgacg gttgacaaca taagttttta
      721 ataatatcct ttacttggaa cttggataaa ttatctataa ggaggattga ttatggttag
      781 tccatttaag gagataaagg ttgatgaagt actggttaag atctataaaa acagggacga
      841 aatgggaaag gcagcatctc ttgatgttgc cgaaaggata agggaaatag ccagggtaaa
      901 agatacgata aatatcgtct ttgcagctgc tccatcccag aatgaatttc tctcctattt
      961 aaatatggaa aagctggatt ggaataagat aaatgccttt catatggatg aatatatagg
     1021 attgcctgcc gatgcccccc agtcatttgc cagctttctt aaggaaagac tcttctccaa
     1081 aaagccattc aaaaatatct tttatataaa tggaaatgct gtcagtcctg aggaagaatg
     1141 taacagatta agcaggctct tagaagataa cccgttagat ataacatgta tgggaatagg
     1201 agagaatgga cacatagcct ttaacgaccc ccacgtggca gattttgacg accccaaaaa
     1261 ggtaaagatt gtggatttag acgaaaaatg cagacaacaa caggttaatg acggctgttt
     1321 caaagatata gatgatgtcc cgaggtatgc gataacaatg acaataccca cactggtatc
     1381 ctccccattt atattctgta ttgtgccagg taagcagaag gcagaggcgg taagaaacac
     1441 tctgcttgga ccagtaagcg agtcctgtcc agcatcgata atcagaagat gcaaaaattc
     1501 tatcctgtat ctggaccttg attctgcttc aaaattagaa ctgtagaaaa acgaggggtc
     1561 agcatgctga ccccctatta cttctaaacg ggattgataa ccctgccgtt atcaaaaaat
     1621 ttatatatca ggatgtcact ctgctacagg tatatagttt atctcagaac caactacaat
     1681 atttccatct tcaacggtta aaaccggaat agactccagc cgaatcttct ttttaccccc
     1741 atcaggtaag cctacttcta taagagattc aaaaggtttt ggaccaggaa aagaaaatgc
     1801 aatctggtaa atcctggaaa tgtccacctc tccaacaact atctttttac ccaaaatgtt
     1861 aaatgctaca tcaaagccta ccttctctac ctttatactg atcttgtttc cattaggatc
     1921 atatttaatg gaaacctcac catgagcagg aacggagtaa tttacagggg gagttttaag
     1981 cgatactgtt acatctgcta gaaactgtgc cttacctgca cttaaaacta ttctcgggtt
     2041 ctttaccacc caggtatatt caccagttat cccaaggatc ttaaatgtgt cagtcttttt
     2101 agtctcccct ataacaagca aaaacttatt aagagcatcc tggctgatca ctatactggc
     2161 gtcagcttca ttccccatag ctatggatgg gacattgaaa ataatcagaa tcagaaaaac
     2221 aactaaagtt gacctcaatg ccttcattat aacctccaaa attaaaattt gtaaggacta
     2281 ttatagccca gaaatttaga atctgtcaac agaaggaaag acaaagcttt tcataatttt
     2341 acatcatccc ccatgccctc tttattatct cttccctttc cagctcatcc ctggcagacc
     2401 ttgcaagttc gaatagttcc ctttcaatcc ggtccctatt ttctaaatag gcagtcctat
     2461 ccctattccc ttctgcaacc tgctctatag tctctttttg cctcttccag taagcaactg
     2521 cggcatcaac ctcatcctca ctgtaaaaga ggtccgggtt cttaacaaac catagaggct
     2581 tatatatgga gagacagggg agagaactgc cggtagcaaa gatatttatc tctccattta
     2641 taagttctac aacaagactc cccgtcgttt cacttgatat aatgcttttt gaatgcatgc
     2701 atatattttt catcgaaccg tataagatat tcttgctgtt cagattatga tgatttctaa
     2761 ggatagcttt aaaatcctct actgtaagcc ttcccttctt ttcttccagc atacctgcag
     2821 tcaggctctg ccggaaatct ccacaactga atctggtaat taatctattc tcgtacttct
     2881 tcttaaaatc tttgatactt aaggcatttg agatactcct cacatcttta acctcttcta
     2941 ccgcccagta agacccggct gtctccagaa cccaggcata attaaaatcg gcaatgagaa
     3001 atgaattatc gtacctcagg tccttagtat aaccacattt tccaccctgc ccataacgct
     3061 ctataagata gacgatatta tcaaccgcct cttttgccgt tttgctcctc tcaagggcaa
     3121 gtctgaggat atccatcccc aggagtgccg gcggaccctg tttctgattg gtaaataccg
     3181 cctcgtttcc tatcacaaca ccattctcgt ttacccccat ctctgcaccc catatccatg
     3241 atggcttaca gaggatacag tcatacgtta tctctgattg ttcaacttca atatacgtac
     3301 acctgacctt ttctcccacc ctgtgctctt tagctgggac ccgtattata atatgtggtt
     3361 cttccttctc ccggtcacta tttttgccga acaaaacaga accatctgag gtagaatttc
     3421 tcaaggctac aatggtatca cacatctcat cagcctccta tcttcttttt catcctgaag
     3481 aattcaaaac tcttaccttt aatctttaca acagatgacc tctctcctgc attgtatcca
     3541 agcttctcgt ataagcttat agctcctcta ttatcagcct ccacatccag caccatcatc
     3601 ttacagcctc tctcacctgc ctcttcttcc accttttcca agagcctggt tcccaatcca
     3661 gaacatctaa attcaggata tacagcaata ttacttatat aatagtcccc tgctaccaga
     3721 tttcccagta tactctgtac cctgagcagg tatggaaccc gtgtaagaat atcccagcca
     3781 agatatttaa ataagagaaa cccggtatat aactcctccc gctccctctc cttccaggta
     3841 tacccaagaa ccatacctgc tactttattc tccatctcaa taaaatatga atgctcaaaa
     3901 ctgaaaatat tcctgggata ccggaataaa ttctccatca cctctaagac attatcgcca
     3961 aataatgacg gaaagaagac aggagcagag agcagaataa gatgggaaaa atcataggca
     4021 tcctctatct tacccgttct tataatgaga tcattcatag atatttaaag gttctcaggt
     4081 cacatatcag gatgcagagt acctataatc tttcttgcca tctcctcttt atcttttctg
     4141 taacatatcc attctgtggg gaaaaatttc cagtcctcgg gacgggaaga atcgagaatt
     4201 tgagtaaagt gacctcccac tccttcaata aggatatcca gtacacctcc ctcattttct
     4261 tcaaaccctt ttataaactc cagtatcttc tcttttccct cttcggtgag ttttgattct
     4321 tcgttccaga cccattctat attatccata tctcctcctg tattttcttt atattgtaac
     4381 acagatgatt gttatatttt atcagttatt cttcatcaag aaaatctatt ccatctattt
     4441 ttttgttttt tacacttgat atctttataa tttcagtaat gaagtcatta tctatgtcga
     4501 tcgttgtacg catcattaac ttccatatat gcataaaaag gaaatctggg ggagattatt
     4561 tttgaagact agatcagact atgttataat tttattagat tcttaggttt aagcgagatg
     4621 cagatagaaa tattagaaga gatattaatt
//
LOCUS       DAOTKU010000074         4643 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_16072_length_4643_cov_2.692023, whole genome shotgun sequence.
ACCESSION   DAOTKU010000074 DAOTKU010000000
VERSION     DAOTKU010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4643
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16072_length_4643_cov_2.692023"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..736
                     /locus_tag="P5310_02725"
     CDS             <1..736
                     /locus_tag="P5310_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32311.1"
                     /translation="YWRHNLGADPIGYCPVGVVLHGEVKTGVHIRQGGVSINTISVSV
                     VNDYVDRTVSGEVEIITPEGWRSYPQTIEYNLEPGEHLTKDVVISFITDGSLRYRPQP
                     DKGIITARLVHDGQVIEDIMEVGGENLLDYQVDFDEATGEITVSLRNPNCDEICGEVF
                     VVSPIELWSKELTGQISLGCISPRSRGFSLPGNGAIDLSFKLELPEEDPQALPSSAWA
                     IVKLAYNGKVSYIPVFGNAIRYECPQ"
     gene            complement(711..2171)
                     /locus_tag="P5310_02730"
     CDS             complement(711..2171)
                     /locus_tag="P5310_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HRU32312.1"
                     /translation="MDQEYTFNFNFTKEDTKKHFPITFEVKEGAREIVIEGSYQPEHP
                     GKEESWELAKEAVRIYLSQGDSPLDVDQLPRHILEKVMEEYIPVRNLINFRIFDPLGN
                     FRGSGDNRISKGIPIKIGKDVSSFGCVSGEIYPGIWKLIVETHAVVNYCKLELKIEIN
                     DTERDDVPELAKPYRPVLRDYPVKRGWIKGDFHSHSVHSDGSLSIEDLVQNAIDRGLD
                     FIFLTDHNKISGWETAREEKFSLLPGIEFTTFWGHFTSFGITEYIEWDKINPDSGIRE
                     VSELVHSQGGLFCVAHPFTIGDPVCTGCRCIMDIDWNYVDALEVWAGTFSQRRHENTE
                     ALRLWRNLLNHGYRIAGIGSTDIHSLKDIYPDVPVTYVLVEELNLINLLDSIKKGRVY
                     ISRGPEVSFNINGASMGDTIKVSEIPLKLRYSISRASDLRVISNGKEVSSITNTSSGE
                     ITLDITDPGYIYLEFWKRGELIALTNPIFIEGIHSV"
     gene            complement(2270..4219)
                     /locus_tag="P5310_02735"
     CDS             complement(2270..4219)
                     /locus_tag="P5310_02735"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRU32313.1"
                     /translation="MSKILKYFLVTLLSISLVTGIISVSFSQQKVAPGQYATIAEYQK
                     VSGKKITKFSEAPQLADLVKQGKLPSVEKRLSEEPAVVEPIEEIGQYGGSLQGPHLGR
                     SPQYTRITYEPLVRWNLETDKVIPNVAKSWKISNGGKVITFYLRKGMKWSDGEPFTAD
                     DIIFWYEDITLNKDLTPVFPAWLIAGGKPGKVEKVDNYTVRFVFDVPYTFLLQRIASG
                     QDCYAPKHYLKKFHPKYTSMDEIMKQAKEEGFDAWYKLFSTKATRWQNKALPVVTAWK
                     VITPLSDAVFTAERNPYYWKVDPAGNQLPYIDKIVWTLSPDREVRVMKHISGEASIGY
                     YDTFESDYTIVMENQQKGGYRVLKWLPALTNVGTLFINQNVKDPVLKKLFTNVQFRRA
                     LSLAINRNEIAQLIYSGNATPRQAVPFKGSPYYEERFEKSYIEYNPKKASQMLDQVGL
                     AKRDKDGYRIGPDGKTLEILIEVLPGIGPEPDIAEMIKGYFRDVGLKVSVKTEETSLW
                     VTRAKAGEQQIGLYASSGAYYPLLDPVWFFPVSDTCYWAPLCGLWYSSGGKSGEEPSG
                     EIKQLLNIYDQAVLAVGEKERTLLIKRALDIYSKNLWTIGVVAPNTRLVIVKNELRNV
                     PEVTIHENINNCPGHTNPEQWFFKK"
BASE COUNT         1303 a         1011 c          848 g         1481 t
ORIGIN      
        1 atactggagg cataatttag gtgctgaccc tataggttac tgccctgtcg gggttgttct
       61 tcatggtgag gtaaagacag gtgtccatat ccgacaggga ggtgtaagta taaatacaat
      121 atctgtgtct gtggtaaacg attatgtaga ccgaactgta agcggagagg tggagataat
      181 cactcctgaa ggctggaggt cctatcccca gactattgag tataacctgg aaccgggaga
      241 acatctgacg aaagatgtgg tgataagttt catcactgac ggaagtcttc gttatcgtcc
      301 acagcctgat aaaggtatca ttacagcaag gcttgtacac gatggtcagg taatcgaaga
      361 tattatggaa gtaggaggag aaaatctgct ggactatcag gttgattttg atgaagcgac
      421 aggagagatc acggtttccc tgaggaatcc aaattgtgat gaaatctgtg gagaagtatt
      481 tgttgtctct ccaattgaac tctggtcaaa ggaactaaca ggtcagatat ctctcggatg
      541 tatatcgccc agaagcaggg ggttttcact cccaggaaat ggagcaatag atctcagctt
      601 taaactggaa cttccagagg aggaccctca agcacttcct agttctgcct gggctattgt
      661 aaagcttgcc tataatggga aggtatctta cattcctgtt ttcggtaatg ctatacgcta
      721 tgaatgccct caataaatat cgggttagtt agagctataa gttctcctct cttccagaat
      781 tcaagatata tataacctgg atcggtaata tcaagtgtta tttccccgga agacgtattg
      841 gtaatagaac tgacctcctt accattggag ataactctaa ggtcagatgc tctggagatt
      901 gagtatctca acttcagggg gatttcagat acttttatgg tatcacccat agaggcacca
      961 ttaatattga aacttacttc aggacctctt gagatatata cccttccctt ctttatagaa
     1021 tccaatagat taataagatt cagctcttcc actaagacgt aagtcacggg cacatctgga
     1081 tatatatctt ttagagaatg gatatctgta gaacctatac ctgctatccg ataaccgtga
     1141 tttaaaaggt ttcgccagag tcttagtgcc tctgtgtttt cgtgtctcct ctgactgaat
     1201 gtcccagccc atacttcaag ggcatctaca taattccaat ctatatccat aatacatcta
     1261 caacctgtgc atacagggtc acctatggta aatggatgag ctacgcagaa gagccctccc
     1321 tgggaatgga ccagttctga aacttctctt atcccactat cagggtttat cttatcccac
     1381 tctatatatt cagttatacc aaaggaggta aaatgccccc agaaagtggt aaattctatt
     1441 ccgggtaata gggaaaattt ttcttccctg gcagtttccc atccagagat cttgttatga
     1501 tctgtgagaa agataaagtc aagtccccta tcaatggcat tctggaccaa gtcttctata
     1561 gataaacttc catcactatg gacagaatgt gaatgaaaat ctcccttaat ccaccctctt
     1621 tttaccggat aatctctcag gacaggtcta tagggttttg ccagttctgg tacatcatcc
     1681 ctttccgtat cgtttatttc tatctttaat tcaagtttac agtagttaac aactgcatga
     1741 gtctcaacta taagtttcca gataccagga tatatctccc cagaaacaca accaaatgaa
     1801 cttacatctt tgcctatctt tattggaatc cctttggaga ttctgttgtc tccactcccc
     1861 ctgaaattac ccaatgggtc gaatattctg aaatttatga gatttcgaac tggaatatat
     1921 tcttccataa ctttttctaa gatgtgccgg ggtagctggt caacatctaa aggagaatcc
     1981 ccctgactta aatatatcct tactgcctct tttgcaagtt cccaggactc ttccttaccg
     2041 ggatgttcag gctgataaga accctctatt actatctctc ttgcaccctc ttttacctcg
     2101 aatgttatag ggaaatgttt tttggtatcc tctttcgtaa agttaaaatt aaaagtatac
     2161 tcttgatcca tatgaatctc ctctcttacc aaattttaac tggataattt ctgttagaca
     2221 ttcttaatcc tcaaaataaa aaaagggggg tacccccctt ttttggaatc tattttttga
     2281 agaaccactg ttctggattt gtatgtccag ggcagttatt tatattttca tgaatagtaa
     2341 cctcaggaac attcctcagc tcattcttaa ctataactag ccttgtattt ggagctacta
     2401 caccaatagt ccagagattc ttggagtaga tatccagagc tctttttatt aacagtgtcc
     2461 tttccttttc tccaactgct aataccgcct gatcatagat gttcagtagc tgctttattt
     2521 ctcctgatgg ctcttcgcca gactttccac cagatgaata ccataatcca cacagaggag
     2581 cccagtaaca ggtgtcagac accgggaaga accagacagg gtctagaagt ggatagtaag
     2641 ccccggatga tgcatagagt ccaatctgct gttcacctgc tttagccctt gtcacccaga
     2701 gtgacgtctc ttctgtcttg acactaactt ttaatcctac gtccctgaaa taccctttta
     2761 tcatctctgc tatatccggc tcaggtccta ttccaggtaa gacctcaatc aatatctcca
     2821 atgtcttacc atcaggtcct attctataac catctttatc tcttttagct aaccccacct
     2881 gatctaacat ctgactggct tttttggggt tatattctat gtaagatttt tcgaacctct
     2941 cttcatagta cggtgagcct ttaaatggaa ctgcctgcct gggtgtagca tttcctgaat
     3001 atatgagctg ggcaatttca ttacggttaa tagctagaga tagagccctt ctaaactgaa
     3061 cattggtaaa taatttcttc aatacagggt cttttacatt ctgattgata aatagagtcc
     3121 caacattggt aagggctggc agccatttta gaactctata tccacccttt tgctggtttt
     3181 ccataacaat ggtatagtcg gactcaaaag tatcatagta acctatactt gcctctccac
     3241 ttatatgctt cattaccctt acttctctgt ctggagaaag tgtccagaca atcttatcaa
     3301 tatacggcaa ctgatttcca gccggatcta ccttccaata atatggattt ctctcggcag
     3361 taaacacagc gtcgcttagt ggtgttataa ctttccatgc ggttactact ggaagggctt
     3421 tattctgcca tcttgttgct tttgtactaa atagtttata ccatgcgtca aaaccttctt
     3481 ctttagcctg cttcattatt tcatccatcg aggtgtattt gggatgaaac ttcttaagat
     3541 agtgcttggg ggcataacag tcctgtccac tggctatcct ttgaaggagg aatgtgtacg
     3601 gaacatcaaa tacaaatctc acggtgtagt tgtccacttt ttctacctta ccaggttttc
     3661 caccagcaat caaccaggct gggaatacag gggtgaggtc tttattcagt gttatatctt
     3721 cataccagaa tataatgtca tctgctgtaa atggttctcc atcagaccat ttcattccct
     3781 tcctgagata gaatgttatc acctttccac cattacttat cttccagctc ttagctacgt
     3841 taggaataac cttgtctgtc tctagattcc atctgacaag tggctcatag gttattctgg
     3901 tatactgagg agatctccca agatgcggac cctgtaagct tccaccatac tgacctatct
     3961 cctctatcgg ctccaccacc gctggttctt ctgatagcct cttctctacc gatgggagct
     4021 tcccctgttt tactagatct gccagttgtg gagcctcgct aaatttggtt attttcttcc
     4081 cagagacctt ctgatactca gctatagtgg catattgtcc tggagcaact ttttgttgtg
     4141 aaaaagaaac agagattata cctgttacta aagaaatact aagtagtgta actaaaaagt
     4201 actttaaaat cttactcact tttatcaacc tccttttata gaatattgta aattcccgga
     4261 aactaaaatt ttttaatctt acttatattt ataataaaaa tacatctcac ctccttaacc
     4321 atatttattc cattaaactc tataaatcat ctgcatccta ccccacaaaa aacttaccag
     4381 cagttcccga gaatctatag aatattttaa catattttaa tataaggggg aagactttat
     4441 atggtttaca tctatggaaa atccattgac tctgtaagga gaagatgata taagcatcca
     4501 gaaagatgga aggacaagag attctaattc atagatttaa aaatataaag ctatgcgttg
     4561 gtggaatcgt agttcttaat aacaggattt tatttgtccg tcagacatat gggaacctta
     4621 aaaatgtttt actgaacaaa act
//
LOCUS       DAOTKU010000075         4628 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_16119_length_4628_cov_4.031271, whole genome shotgun sequence.
ACCESSION   DAOTKU010000075 DAOTKU010000000
VERSION     DAOTKU010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4628
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16119_length_4628_cov_4.031271"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="P5310_02740"
     CDS             <1..272
                     /locus_tag="P5310_02740"
                     /inference="COORDINATES: protein motif:HMM:NF037628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32314.1"
                     /translation="TPIFINQLMLLEPFGVGNPRPVFIARGVNVIGKEMQNNSKRIII
                     EENGNIFEVSASDDTIPTDSPIDLVYSIKGMGKIIGIGGLKSGSR"
     gene            259..780
                     /locus_tag="P5310_02745"
     CDS             259..780
                     /locus_tag="P5310_02745"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008789829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HRU32315.1"
                     /translation="MEVDELVRYIRSIPDFPVKGVLFRDITPLLKDRDAFKTAVSGLA
                     DNFRDCNVDLVSAVEARGFIIGAPLAIELSAGFVPIRKPGKLPYDSIREEYSLEYGTN
                     VLEMHRDAIKPGEKVLIADDLIATGGTAIACKNLIERSGGIVVGFVFLVELTEFNARE
                     QLKGYDVYSLLKF"
     gene            774..2852
                     /locus_tag="P5310_02750"
     CDS             774..2852
                     /locus_tag="P5310_02750"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HRU32316.1"
                     /translation="MLEKFDKDGKNLIISAYEFSLAAHNGQKRRSGEEFFVHPLEVAK
                     ILSGMDVDAVTIASSLLHDVVEDTDVSIEEIEKRFGPEVASLVDGVTKLNRLTGLTGE
                     ERQAENLRKMFLAMAKDVRVIFIKLADRLHNMRTLSYLPVEKQKIIAKETLDIFAPLA
                     HRLGMWRIKWELEDLSFKYLESEKYREISRLVAKTRKEREEQIEEAISIIKSNLETLG
                     IKADIQGRPKHLYSIYSKMKRDNLEFDQIYDLLGIRVIVETTKDCYAVLGIVHSLWKP
                     IPGRFKDYIAVPKSNMYQSLHTTVIGPKGEPLEVQIRTFEMHRVAEYGIAAHWSYKEG
                     KTDKEFDMKLSWLRQLLEWQKDLQDAREFVEQVRVDLFEDEVFVFTPKGDVVSLPKGS
                     TPVDFAFRIHTEVGNHCVGAKVNGRIVSLNYELKSGEIVEILTSKSATPSLDWLSFVA
                     TNSARNRIRQYFKRLQKEENIVRGKETLERELKRIQGDLPDKMRELDWVPLLEAFNLD
                     TPEALFLAVGNGEITSSQIQQVLQKSTKEERVVGLPRRDEGLGISVDGVDNLLVRLAR
                     CCSPLPGDEIIGYVSQGRGITVHRKECVNVKNLPEEKLINVYWNVDNIHRFNARIDVY
                     ARDRMGLLKDILTEVSNLGSNVYDTRAVVKNGRVNIYIVLEVQNIHHCEAIMRAIKGV
                     RDVITVGRHS"
     gene            2853..3293
                     /gene="dtd"
                     /locus_tag="P5310_02755"
     CDS             2853..3293
                     /gene="dtd"
                     /locus_tag="P5310_02755"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004632390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HRU32317.1"
                     /translation="MRVVIQRVKSADVKVDGNVISSIGKGFLLLVGISKTDTEREIGE
                     MAKKIPYLRVFEDENGKMNLSLKDVGGEILSVSQFTLYGDTSRGRRPGFERAAGRDTA
                     IELWNKFNQILRESGITVKEGIFGEHMEVSLVNDGPVTIIMEEG"
     gene            3294..4322
                     /locus_tag="P5310_02760"
     CDS             3294..4322
                     /locus_tag="P5310_02760"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRU32318.1"
                     /translation="MNSYERVLAVISGRIPDRVPIFELLIDRKVIRKITGREDYLDFC
                     ERYPIDIVLTNTPSKLYEEKIINPENKIIVNEWGVVRQYTEEEVSIPLEGPIKTPEDL
                     EHYEAPDPYKERRYEELREIVKRFKGKKFIGMHLHDALNYPLYLRGAEQLFIDTIENP
                     ALVHRLVDISVRHNIAIAEKAIELGADFIILGDDYGASNGPMMSPRSFREFFLPGIRR
                     IVQSVRSKGAFCFKHCCGNINAIIDDMVSTGISALHPLDISAGMDILAVKNRFTNLTV
                     MGGVNCGAPLTEWSKEALVDEVRSVLERMKPGGRYIISSSNSIHSKVKPENLLSMWET
                     VERYGRYD"
     gene            4328..>4628
                     /locus_tag="P5310_02765"
     CDS             4328..>4628
                     /locus_tag="P5310_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32319.1"
                     /translation="MRFLWRKKKTHTYEYYYNQYPARIKEHLSIGDIHFVERVIAICR
                     DAGVGMSSEEIWEALLRYDGSIDADIKLDVIGWIGQLRSFVMESKEAYPSGLSRFF"
BASE COUNT         1438 a          740 c         1209 g         1241 t
ORIGIN      
        1 taacccccat ctttataaat caactgatgc ttcttgagcc attcggagtg gggaatccaa
       61 gacctgtttt tattgccaga ggagtaaatg ttatcggaaa ggaaatgcaa aataattcaa
      121 aaagaattat aatagaagag aatggaaata tctttgaggt ctctgcctca gatgatacta
      181 tacctacaga tagccctata gacctggtct acagcataaa gggtatgggc aagataatag
      241 gaataggagg attgaaaagt ggaagtagat gaacttgtaa ggtatataag aagtattcca
      301 gatttccctg tgaagggagt actttttaga gatattactc cattacttaa ggatagagat
      361 gcttttaaaa ctgcggtctc tgggctggct gataacttta gagattgtaa tgttgatcta
      421 gtgtcagctg ttgaggccag aggatttata ataggagctc ctctggctat agagctatct
      481 gcaggttttg tccctataag aaagcccggg aagttaccat acgatagcat aagagaagaa
      541 tactccctgg agtatgggac caatgtactg gagatgcata gagatgctat aaagcccggt
      601 gagaaggtcc tgatagccga tgaccttata gctaccggtg gtactgctat agcctgtaaa
      661 aatttgattg aaagatcagg tggtatagtg gttggttttg tctttctggt tgagcttacc
      721 gaatttaatg cgagggagca gttaaaagga tatgatgtct attccctttt gaaattctag
      781 agaaatttga taaagacggt aaaaatctca taatatctgc ctacgaattt tctcttgctg
      841 cccataatgg gcagaagaga aggtcagggg aggagttttt tgtccatcct ctggaggtag
      901 ctaaaattct atccggtatg gatgttgatg cagttactat tgcctcttcg ctccttcatg
      961 atgtggtgga ggatactgat gtctccatag aagagataga aaaaaggttt ggtcctgagg
     1021 tcgccagtct tgtggatggt gttacaaaat taaatagact tacaggactg accggagagg
     1081 aacgtcaggc tgaaaatctc cgtaagatgt tccttgctat ggctaaagat gtgcgggtta
     1141 tatttataaa acttgccgac agattgcaca atatgagaac gctctcctat ctcccggtag
     1201 aaaaacagaa gataatagca aaagagactc tcgatatatt tgcccctcta gcacacaggc
     1261 tgggtatgtg gagaataaag tgggaactgg aagacctcag cttcaagtac ctggagtctg
     1321 agaagtatag ggagatctcc agattggtgg ctaagacgag aaaagagagg gaggagcaga
     1381 tagaagaggc tatctctata ataaagtcta atctggaaac cttggggatc aaggcagata
     1441 tacagggacg tccaaagcat ctctacagta tatattcaaa gatgaagagg gataatcttg
     1501 agtttgatca gatatacgac ctgcttggta tcagggttat agtagaaacc acaaaggact
     1561 gttatgcggt tcttggtata gtccattctc tctggaagcc tattcctggc aggtttaaag
     1621 actatattgc ggttccaaaa tccaatatgt atcagagtct acacaccact gttataggtc
     1681 ctaaaggaga gccactggag gttcagataa ggacattcga gatgcataga gttgccgaat
     1741 atggtatagc ggctcactgg agctataaag agggaaagac tgataaggaa tttgatatga
     1801 aactctcctg gctgagacag ctcttagagt ggcagaagga cctgcaggat gccagagagt
     1861 tcgtagaaca ggtcagggta gacctctttg aagatgaggt ctttgtcttt acccccaagg
     1921 gtgatgttgt atctctgccc aaaggttcta ccccggtaga ttttgcattt cgtatccata
     1981 ctgaggttgg aaatcactgt gttggggcta aagtaaatgg cagaatagtc tctctgaatt
     2041 atgaacttaa aagtggcgag attgtggaga tactcacatc taaatctgct accccaagcc
     2101 tcgactggct ctcctttgta gctactaact ctgctaggaa caggataaga cagtacttta
     2161 agaggctgca gaaggaagag aatattgtga gggggaagga gactctagag agagagctta
     2221 agagaatcca gggggactta cctgataaga tgagggagct tgactgggta cccctgcttg
     2281 aggcgtttaa tctggatact ccagaggctc ttttccttgc tgtgggaaat ggagagatta
     2341 cctcttctca gatacagcag gtattacaga agagtacaaa ggaggaacgg gtagttggat
     2401 taccaaggag agatgaggga ttagggatat ctgttgatgg ggtagataat ctccttgtaa
     2461 ggttggcacg ttgctgcagt cccctgcctg gagatgagat tataggttat gtctctcagg
     2521 gaagagggat tacagtccac aggaaagagt gtgtgaacgt aaaaaatctt cctgaagaga
     2581 aactgatcaa tgtatactgg aatgtcgata atattcaccg atttaatgct cgaatagatg
     2641 tgtatgctag ggataggatg ggactgttaa aagacatact caccgaggtc tccaatcttg
     2701 gaagtaacgt atacgataca agagctgttg taaagaatgg aagagtgaac atatatatag
     2761 tacttgaggt acagaatatc catcactgtg aagctattat gagggctata aaaggggtaa
     2821 gggatgttat tacggttggg aggcattcat agatgagagt ggtgatacag cgagtaaagt
     2881 ctgctgatgt aaaggtggat ggtaatgtca tctccagtat agggaaggga ttccttcttc
     2941 tggttggtat aagtaagaca gatactgaaa gagagatagg tgagatggct aaaaagattc
     3001 catatctcag ggtctttgag gatgaaaatg gcaagatgaa tctgagtcta aaggatgttg
     3061 ggggagagat actctccgta tctcagttta ccctttatgg agatacatcc agaggacgcc
     3121 gtcctggttt tgaaagagct gctggaagag ataccgccat cgaactctgg aataagttta
     3181 atcagatctt aagggagagt ggcattacag taaaagaggg tatctttgga gagcatatgg
     3241 aagtatcact cgtaaatgat ggtccggtta ccattataat ggaggaggga tagatgaatt
     3301 cttatgagag agttttagcg gtaatctcgg ggaggatacc tgacagggtt ccgatattcg
     3361 aactcttgat agatagaaag gtgatcagaa agataactgg aagagaagac tatctggatt
     3421 tctgtgagag atacccaata gatatagtat tgacaaatac accatcaaag ttatacgaag
     3481 aaaagattat aaatccggag aataagatta ttgtaaatga gtggggagtg gtaagacagt
     3541 atactgaaga agaggtttct ataccactgg aaggtccgat aaagacacca gaagaccttg
     3601 agcattatga ggctcctgac ccatataaag agagaagata cgaagagcta agagaaatag
     3661 tgaagagatt taagggcaag aagtttatag ggatgcatct ccacgatgcg ttaaattatc
     3721 ctctctatct gagaggggca gaacagttat ttatagatac gatagaaaat ccagcacttg
     3781 ttcacaggtt agttgatata tcggtaagac ataacatagc gatagccgag aaggctatag
     3841 aattgggggc tgattttata atacttggtg atgattatgg tgccagtaat ggacccatga
     3901 tgtctccaag atctttcaga gaatttttct tacctggtat aagaaggatt gtacagtctg
     3961 tcagatctaa aggtgccttc tgtttcaagc actgctgcgg gaatataaat gccattattg
     4021 atgatatggt ctctaccgga atatctgccc ttcacccact tgatataagt gccggtatgg
     4081 atatcctggc agtgaagaat agatttacca atcttactgt tatgggtgga gtaaactgtg
     4141 gggctccatt gacagagtgg tcaaaagagg ctctggttga tgaggtgagg tcagtgcttg
     4201 agaggatgaa gcccggcggg agatatatta tttcttcaag taactcgata cacagtaagg
     4261 taaagccaga gaaccttctt tctatgtggg agacggtaga aaggtacgga aggtatgatt
     4321 aatttttatg agattcctct ggcggaagaa aaaaacccat acatacgagt attattataa
     4381 tcaataccct gccaggataa aggaacatct gagtatcggc gatatacact ttgtagagag
     4441 ggtaattgcg atatgcagag atgccggtgt tgggatgagc tcagaggaga tatgggaggc
     4501 acttctgagg tatgatggaa gtatagatgc agatataaag cttgacgtta taggatggat
     4561 aggacagctg agaagctttg ttatggagtc caaagaggcg tacccttcag gtctatccag
     4621 atttttca
//
LOCUS       DAOTKU010000076         4626 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_16130_length_4626_cov_3.652155, whole genome shotgun sequence.
ACCESSION   DAOTKU010000076 DAOTKU010000000
VERSION     DAOTKU010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4626
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16130_length_4626_cov_3.652155"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(173..1351)
                     /locus_tag="P5310_02770"
     CDS             complement(173..1351)
                     /locus_tag="P5310_02770"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRU32320.1"
                     /translation="MDRLKDTLKKDVASSILAGIFLASTTPFFGVIARRYFSASPLHI
                     SLISIASGVGQLFTFYWGYIIRSRRDKLKMYVIPNIVARGVFLFTPIIYSISSFLLII
                     FLYQIISCMATPAYVEVVKAMYPDMLRGRIMGLVKMINNLVVIAVTPLSGQAISSIGF
                     RWVFFVSGIAGIISTLIFSRIKIREVHRETSVPVSIKTILTIPFLDKKFGQYLSVFFM
                     YGLGNWLSNPIYPIILVDKLQASTLQVGYISTITSILSAIFYIYWGRYIDKKNPVRVL
                     FLVFSLDFMIPLGYLLSSIKPTLIFVFISAGISGIVNAGVDLAVMNTILMLAPKEEIA
                     SYMALHSTFVGIRGIIGPFLGASLYPVIGPIGIFSINIATALSGGFLMYRFCNSLTTT
                     "
     gene            complement(1344..1610)
                     /locus_tag="P5310_02775"
     CDS             complement(1344..1610)
                     /locus_tag="P5310_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HRU32321.1"
                     /translation="MRLDVFLKASGVIKRRSLANELCDRGLVSVNGAKGKAGRVLKEG
                     DIVDVKVGMYKYKLEVLSLPERKVNRLTEENFRILEKSVEKVDG"
     gene            complement(1607..2542)
                     /locus_tag="P5310_02780"
     CDS             complement(1607..2542)
                     /locus_tag="P5310_02780"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRU32322.1"
                     /translation="MTTENQAQEAEKGHLFGNKYLWILGIVIALAIASNVIMYIWFTS
                     PERIGVAEVNGEVITKDEFIKAIIAQNGQSALDWIIENKLIYQEAKKEGISVSDEEIE
                     SRMSEIRSSFGSQESFLSILSLYGLTEEAFKEQLIPRMLAEKIIMKDKDISDKDLIEF
                     FAKNKSSFDEKEQVKLRHILLKTLEEAQQAEEELRNGKDFGTVAKEMSIDTATSSNGG
                     EMGWVGKGVLDPALEKVVFSLNKGERTSVVKTSFGYEIAEVLDKKAARSVTFEEVKDR
                     VKEAYIEDIVQQNYSDWVQGLKSVADIKYYISPGK"
     gene            complement(2556..3407)
                     /gene="purR"
                     /locus_tag="P5310_02785"
     CDS             complement(2556..3407)
                     /gene="purR"
                     /locus_tag="P5310_02785"
                     /inference="COORDINATES: protein motif:HMM:TIGR01743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HRU32323.1"
                     /translation="MSNRLSRQERLIILSQELIKRSGELISLDELAEKYKIARSTISE
                     DLSLIREALEVWGRGEMQSSSGVGGGVRFVPVIGSEEAKKFLHDVKGRLEERGRVLPG
                     GFLYTTDLLTHPGITSKIGEMFATIFNKVSPDVIVTMETKGIPAAIMTARYMGVPLVI
                     IRRENRPTEGSTVSINYISGSTQKVQLMTLGRRALEGRERAIFIDDFLRGGGTVRGAI
                     ALLKEFNAQVVGVGIVILGSPGIKDAFSIPITPLFVLESIDETLSEAKVRISESIYEF
                     FGWKKED"
     gene            complement(3397..4143)
                     /locus_tag="P5310_02790"
     CDS             complement(3397..4143)
                     /locus_tag="P5310_02790"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HRU32324.1"
                     /translation="MYNCAVMAGGGKDSLKLFNVSATSKGAIPISGKPMVEYVIEALR
                     SAKSIDKILYVGDIEILSSIKVRIDYSIPDAGELFLNLMNALEFFKGESQILIVTSDI
                     PLIKGYMIDVFLSRCDRSAIFCYSFVRREDSEKLFPKAHRTYVKLKEGSFTGGNIMLV
                     NPTFILRNRELIRGIVSNRKNPFNLAKLIGWNIILRYIMGNLDIPTLEAKSSKILGGK
                     TQAVLMEYPEIGFDVDNQTQLNFVEERLVK"
     gene            complement(4143..>4626)
                     /locus_tag="P5310_02795"
     CDS             complement(4143..>4626)
                     /locus_tag="P5310_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32325.1"
                     /translation="ASSIGCDVPFFLYGGSAMIKGKGTDVTPLPPIEGIPVLVVVPDV
                     EVSTHWAYKNIIVPLYPFRTPSILLKLLKKNQVDVEELNRFLFNRFSDLVFRLPKVNE
                     AKNILESLGVENIALSGSGPTLFSLQEDKEKCNLLVKTLREKFKLRAFSAELCGGIYR
                     "
BASE COUNT         1420 a         1062 c          729 g         1415 t
ORIGIN      
        1 ggaaacgaaa atgattaaaa agcttggact tatcctgttt actttagttt ctcttgtcct
       61 ggccggtggg gccaatctca agtggtggta aaggaggaaa cgaaaatgat taaaaagctt
      121 ggacttatcc tgtttacttt agtttctctt atcctggccg gtggggccaa tcttaagtgg
      181 tggtaagact attacaaaac ctatacatta agaacccacc tgaaagagcg gtggctatat
      241 ttatagagaa gataccgata ggacctataa ctggatataa agaggctcct aagaagggac
      301 cgattatgcc cctgatgccg acaaatgtag agtgcaaagc catataactg gctatctctt
      361 ctttaggagc caacataaga attgtattca taactgcaag atctactcct gcattcacta
      421 ttccacttat tccggcagag ataaatacaa aaataagggt gggctttata ctggacaaaa
      481 gatatccaag tggaatcata aaatccaggg aaaagacgag aaatagaact cttaccggat
      541 tctttttatc tatatatctc ccccagtaaa tataaaagat agcggaaaga atcgatgtaa
      601 tagtagagat atatccgacc tgtagagtac tcgcctgaag tttatccacc agtattattg
      661 gatatatcgg atttgaaagc cagttaccca gcccgtacat aaaaaacacg gaaaggtact
      721 gaccaaattt tttgtctaaa aaaggtatgg tcagtattgt tttgatagat actggaacac
      781 tggtttccct gtgaacctct ctgattttta tcctgctaaa tattagagtg gagataattc
      841 cagctatccc gctaacgaag aatacccatc tgaaacctat agaggatatg gcttgtccag
      901 ataaaggggt tacagctatc accacaagat tatttatcat cttcaccaga cccataattc
      961 tgcctctgag catatcggga tacatagcct taacaacttc tacatatgca ggtgtagcca
     1021 tacatgagat gatttgatat aaaaatataa taagtaagaa cgaactaatt gagtatataa
     1081 tgggggtaaa gagaaaaact cctctggcta ctatattggg aatcacatac atttttaatt
     1141 tgtctcttct tgaacgaatt atatagcccc aataaaaggt gaaaagctgt ccgacaccgc
     1201 tggctattga tataagagag atatggagag gagatgcaga aaaatacctt cttgcaatca
     1261 ccccaaaaaa tggagtagtg gaggcaagaa atatcccggc taaaatactg gaagccacat
     1321 cctttttaag ggtatctttt aatctatcca tcaacttttt ctacagattt ttctaatata
     1381 cgaaagttct cttcagttaa ccggtttact tttctttcag gaagggagag gacctccagc
     1441 ttatatttat acatacctac tttaacatct actatatccc cctcttttaa aaccctccct
     1501 gccttacctt tagcgccatt tactgatact agccctctat cacaaagttc attcgctaag
     1561 gaccgtcttt taataacccc agaagcctta agaaatacgt caagtctcat tttcctgggc
     1621 ttatataata tttaatatca gcaactgact ttaagccctg tacccaatca gaataattct
     1681 gctggactat atcctctata taggcttctt ttaccctatc tttcacttct tcaaacgtga
     1741 ctgatcgagc agccttctta tccaaaactt ctgctatttc atagccgaag gatgtcttaa
     1801 ctacagatgt tctttctcct ttatttaagg aaaatacaac cttctctaaa gctggatcca
     1861 ggacaccttt tcctacccaa cccatttctc caccgttact gctagtagcg gtatctattg
     1921 acatctcttt agctacagta ccgaaatcct tgccatttct caattcttct tctgcctgct
     1981 gtgcctcttc cagcgtttta agaagtatat gtcttaactt cacctgttct ttttcatcaa
     2041 aagagctctt attcttggca aaaaactcta ttaaatcctt gtctgatata tccttatctt
     2101 tcataataat cttttctgcc agcatcctag gtataagttg ttccttaaac gcttcttcag
     2161 taagaccata gagagataag atagatagaa atgactcctg agacccaaag ctgctcctga
     2221 tttctgacat tctactctct atctcttcgt ctgaaacaga gatgccttcc ttcttagcct
     2281 cctgatatat aagtttattc tctatgatcc agtccagagc actctgtcca ttttgagcta
     2341 tgattgcctt tataaactca tctttagtta ttacttctcc atttacctct gccactccaa
     2401 tcctctctgg agatgtaaac catatataca taattacatt tgatgcgatg gctaaagcta
     2461 tcactattcc aagtatccat aaatatttat taccaaagag atgtcctttt tctgcttctt
     2521 gcgcctgatt ttctgtagtc attaattttt ccctcctaat cttccttttt ccagccaaaa
     2581 aattcgtata tagattctga aattcttacc ttagcttcac ttaaagtctc gtcgatactt
     2641 tccagaacaa aaagtggagt tatcgggatg gaaaatgcat cctttatccc gggagaccca
     2701 agtataacta ttcccacacc tacaacctgt gcattaaatt ccttcagtaa agcaatcgct
     2761 cctcttaccg tcccaccccc tcttaaaaaa tcatctataa atattgccct ttccctaccc
     2821 tcaagagccc ttctacccag ggtcatcagc tgaacctttt gggtagaacc agatatatag
     2881 ttaatagaaa cggtagaacc ctcggttggt ctattttctc tcctgatgat aacaagcgga
     2941 acccccatat atctagcggt cataatagca gcaggaatcc ccttagtttc catagtcact
     3001 attacatccg ggctaacctt attaaaaatc gtagcaaaca tctcaccaat cttagaagtt
     3061 atacccggat gtgtaagaag atcggtagta tacagaaatc ctccaggtaa aaccctgcca
     3121 cgttcctcca gtctaccctt aacatcatgg agaaattttt tcgcctcttc agagcctatt
     3181 acaggaacga atctaacccc tccgcctacg ccagaactgc tctgcatctc tcccctaccc
     3241 catacctcta atgcctctct tatcagagaa agatcttcac ttatagtaga tcgtgctatc
     3301 ttatattttt cagccagctc atccagtgaa ataagttctc cagacctctt tataagttct
     3361 tgcgatagga tgattaatct ctcctgccta gagagtctat ttgacaagtc gctcctccac
     3421 aaaatttaat tgagtttgat tatcaacatc aaagccaatc tcaggatatt ccatcaatac
     3481 tgcctgtgtc ttccccccta aaatcttaga agatttagct tcaagggtgg ggatatcaag
     3541 attacccatt atatacctca aaataatatt ccagccgata agtttagcta aattaaaagg
     3601 attttttcga ttagaaacta ttccgcgaat aagttcccta tttctcaaaa taaaggtggg
     3661 atttaccaac ataatattac caccggtaaa agacccttcc ttcaacttta catatgtcct
     3721 atgagctttt ggaaataatt tttcagaatc ttctctcctg acaaaagagt agcaaaaaat
     3781 cgccgacctg tcacatctgg agagaaacac atctatcata taccctttaa ttaatgggat
     3841 atctgacgta acaatcagga tctgggattc gcccttaaaa aattccagtg cgttcataag
     3901 attaaggaaa agctccccgg catctggaat agaataatca atccttacct taatactgga
     3961 aagaatctcg atatcaccta catacagtat cttatctata ctctttgcac tcctgagcgc
     4021 ctcaatgaca tattcaacca taggtttacc tgaaatgggt atagccccct tactggtggc
     4081 agaaacatta aataacttta agctatcttt accaccccca gccataacag cacaattata
     4141 catcacctgt aaattccccc acataactct gcactaaaag ctctaagctt aaacttttct
     4201 ctgagagtct ttacaagtag attacatttt tctttatctt cctggagaga aaataatgtg
     4261 ggtccactgc cagataaggc tatgttttca acacccaaag attcaagaat attttttgcc
     4321 tcattcactt tgggtagcct gaagactaaa tcggagaatc tattgaacaa aaatctattt
     4381 aactcttcaa catctacctg attcttcttt aacaatttta acaaaattga tggggttctg
     4441 aatggataaa gaggtacaat tatattctta tatgcccaat gggtagaaac ctccacatcc
     4501 gggacaacaa ccaggacagg tattccctca attgggggta atggcgtaac atctgtcccc
     4561 tttcccttta tcatagcaga cccaccatag agaaagaatg gaacatcaca gccaatactc
     4621 gatgct
//
LOCUS       DAOTKU010000077         4607 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_16191_length_4607_cov_3.114455, whole genome shotgun sequence.
ACCESSION   DAOTKU010000077 DAOTKU010000000
VERSION     DAOTKU010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4607
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16191_length_4607_cov_3.114455"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1447
                     /locus_tag="P5310_02800"
     CDS             <1..1447
                     /locus_tag="P5310_02800"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HRU32326.1"
                     /translation="AVLEALEKSDAIIGVQDMGAAGITSSSVEMANRGGVGVEIEVTK
                     VPQREKGMTPEEIMLSESQERMLLCVERGKEEGLRPIFEKWGLNFAIIGQVIPEKMVR
                     IKENGNIVGEIPVDILTEMVPAYDLETKIPENLNRLQTVDSIPEIEDYGDVLLRLLNS
                     PNCSTSKWVYEQYDHMVETNTVSIGTGASIIRVKGKKFLIGVTTDGEGRYCYLDPYIG
                     GIMIVAESARNLICIGAEPLAITDGLNFGNPEDPEVAWYFVETVKGMSECARKLNIPV
                     VGGNVSFYNESENYTVYPTPIVGMVGKIENLETLCTPQFKDEGDVIYILGEPGNLGGT
                     LFIEELTGEAKGKPEIDLELEVKLQRLLLKAIREKLIKSAQDISRGGLAIALAECSIW
                     GNMGIDISIESEKSPEEVLFGEAQPVVIITTDANRERYVEEYFKEVPIRKVGRVIKDR
                     FKIKFNRNSIVDLEISLLKNKWNSGIERWMI"
     gene            1435..2823
                     /gene="purF"
                     /locus_tag="P5310_02805"
     CDS             1435..2823
                     /gene="purF"
                     /locus_tag="P5310_02805"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014274366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HRU32327.1"
                     /translation="MDDLKEYCGVFGIYSPNSDVARLTYFGLFALQHRGQESAGIAVS
                     DRKDIVCYKDMGLVSQVFDEKILNILKGDIAIGHVRYSTTGSSTELNAQPMLVSGENP
                     IAIAHNGNLVNSLDIKKELEEKGIKISGTVDTEVMAKKIALYYKNDILSTLLEVLPSF
                     QGAYTMLVLTRDKLIGVRDPYGIRPLVLGKFNGSYVLSSETCGLDIIGAKFIREIEPG
                     EIVVIDEEGIKSTTFAQPDPRLCIFELVYFARPDSKFGTRLAHEIRQEMGRILAQESP
                     VDADLVIPIPDSGIPAAIGYAQQARIPFSEGLIKNRYVGRTFIQPDQRLRELGVRLKL
                     NPLKEVIEGKRIVLVDDSIVRGTTSRQIVNLLREAGAKEIHMRVSSPPTSYPCFYGID
                     TAERGQLISAYKSIEEVRKYIGSDTLRYLSVEGLLRACRGGAESFCTACFTGDYCIPI
                     PQDIKVTKGILE"
     gene            2834..4093
                     /gene="hisD"
                     /locus_tag="P5310_02810"
     CDS             2834..4093
                     /gene="hisD"
                     /locus_tag="P5310_02810"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HRU32328.1"
                     /translation="MKNRIIRDRKDLSNLVLERKKASDVKIAVEEILERVKEAGDVAL
                     IKYSNEFDKANFSSPKDFVVSKEEIEESIDVVGEEFISAVNMAIKNIRDFYSRMPRPQ
                     NWFYLKNGKLSGQMVVPLESVGIYVPGGRAPYPSTVMMAAIPAQIAGVKRIVVTTPKP
                     VPEVLAVCKLLDIYEVYRLGGAHAIAGLAYGTDTITRVDKIVGPGNRFVVSAKKYVFG
                     ETGIEMIPGPSEIVVLADSNANPKFVAIDLLSQAEHDPLAMSILVTDSEDLANRVIEE
                     MGILIRELSTCETAKESWESWGGIVLVNSIDEGIDTVNFIAPEHLELQIKNPASYLEK
                     IRNAGTIFIGEKTPEAIGDYIAGSSHILPTGGSARFSSGLNVLDFLKLINITQYNEIT
                     EDFKSGSIIADREGFSAHKKSLEIRDR"
     gene            4160..>4607
                     /locus_tag="P5310_02815"
     CDS             4160..>4607
                     /locus_tag="P5310_02815"
                     /inference="COORDINATES: protein motif:HMM:NF015042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HRU32329.1"
                     /translation="MLLKMAGYDVIGVTMRIWGGEPTKTGKRHSCYGPEELDDIEDAR
                     RVCERLDIPFYEIDLIKEYREIVLTYFKNEYLKGRTPNPCIRCNQLLKFKALLEKVEK
                     IGIEFDYFATGHYARIEYDSDRDRYILKKGIDERRDQSYFLFSLTQN"
BASE COUNT         1592 a          717 c         1136 g         1162 t
ORIGIN      
        1 agccgtatta gaggcattag aaaagagtga tgcgataata ggtgtacagg atatgggagc
       61 agcaggtata accagttctt ctgtagagat ggcaaataga ggtggggttg gagttgagat
      121 agaggtaaca aaggttcctc agagagagaa agggatgact ccagaagaga ttatgctttc
      181 agagtcacag gaacgtatgc tactatgtgt agaaagaggt aaagaagaag gactaagacc
      241 aatatttgag aagtggggac tcaattttgc tattatcgga caggtgatcc cagagaagat
      301 ggtaaggata aaagaaaatg ggaatatagt tggagagata cctgtagata tcctcaccga
      361 aatggtaccc gcttacgatt tggagacaaa gatcccggaa aatctaaata gattacagac
      421 agtcgattct atcccagaga tagaagatta tggggatgtc ttactcagac ttctaaattc
      481 tcctaactgc tccaccagta aatgggtcta tgaacagtat gaccatatgg ttgaaacaaa
      541 tactgtaagt ataggaacag gcgcctctat aatcagagtg aagggtaaga agtttttgat
      601 aggtgtaaca accgatgggg agggaagata ctgttatctg gacccatata tcggtggaat
      661 aatgatagtg gcagaatcgg caagaaacct tatatgtatt ggtgctgaac cattagctat
      721 aacagatgga ctgaactttg gtaatcctga agatccagag gtagcatggt atttcgtgga
      781 aacggtaaaa ggtatgagtg agtgtgctag aaagctaaat atacctgtgg taggtggaaa
      841 cgtaagcttt tacaatgagt cagagaatta tactgtttat ccaaccccca tagttggtat
      901 ggtggggaag atagagaatc tggagacatt atgtacacct caatttaaag atgagggaga
      961 tgttatctat atacttggag aacctggtaa tcttggtgga actctattta tagaagagct
     1021 tacaggtgaa gcaaagggaa aaccagaaat tgatctggag ctggaggtaa agttacagag
     1081 gctattactt aaggctataa gggagaaact tataaaaagt gcccaggata tatccagggg
     1141 tggattggca atagctctgg cagagtgcag catatggggg aatatgggga tagacatcag
     1201 tatagaatca gaaaagtctc cagaagaagt tctctttggt gaagcgcaac ctgtggttat
     1261 aataacaacc gatgcaaaca gagaaagata tgtagaagaa tactttaaag aagtaccgat
     1321 aagaaaagtt ggaagggtta taaaggatag attcaagata aagttcaaca gaaacagcat
     1381 agtagattta gaaatatctt tacttaaaaa taaatggaac agtgggatag aaagatggat
     1441 gatctgaaag agtactgtgg tgttttcggt atatacagtc caaatagtga cgtggcaaga
     1501 ttaacctatt ttggactctt tgcccttcag cacaggggtc aggagagtgc agggattgct
     1561 gtatcagata gaaaagacat tgtatgttat aaggatatgg gattagtaag tcaggttttt
     1621 gatgaaaaga tattgaatat cctgaaggga gatatagcga tagggcatgt aagatactca
     1681 accaccggat caagcaccga gttaaatgct cagcccatgt tagtctcggg agaaaatccg
     1741 atagctatag cccataatgg aaatctggta aattcgttag acataaaaaa agagctggaa
     1801 gaaaaaggta taaagatttc aggtacagta gacacagagg tgatggctaa gaagatcgcc
     1861 ctctactata aaaatgacat cttatccaca cttttagaag ttcttccctc atttcaaggt
     1921 gcttatacca tgttagtact gaccagagat aaacttatag gggtaagaga cccgtatgga
     1981 ataagaccac tggtattggg aaaatttaac ggaagttatg tactttcatc tgaaacttgc
     2041 gggttagata taataggggc aaaatttatc agggagatag aaccaggaga aatagtagtg
     2101 attgatgagg aagggattaa aagtacaact tttgctcagc cagacccgcg tctatgtata
     2161 tttgaactgg tctattttgc ccgtccggac agtaaatttg gaaccaggct tgctcacgag
     2221 ataagacagg agatggggag aatattagca caggaatcac cagtagatgc tgaccttgta
     2281 attcctattc cggattctgg tattcctgcc gctatagggt acgcacagca ggcaagaata
     2341 cctttcagtg aaggactaat taaaaacaga tatgttggta gaacatttat acagccagac
     2401 cagagattga gagagcttgg agtaaggtta aaactaaatc ctttaaaaga ggtaattgag
     2461 gggaagagaa tagtattggt cgatgattct attgtaagag gaacaacctc aaggcaaata
     2521 gtaaatctcc taagagaagc tggggctaag gagatacata tgagggtcag ttcaccccca
     2581 acatcttatc cttgctttta cggaatagat actgcagaga ggggacagct tatctctgcc
     2641 tataagagca tagaagaggt aagaaaatat ataggaagtg acacccttag atacctcagc
     2701 gtagaaggac ttcttagagc ctgcagggga ggagcagaaa gtttctgcac tgcctgtttt
     2761 actggagatt actgtatccc aatcccgcaa gacataaaag taacaaaagg tatactggaa
     2821 taaggagata aggatgaaaa atagaattat aagagataga aaagatctat ccaatctagt
     2881 cttggaaaga aaaaaggctt cagatgtaaa gattgctgtt gaagaaattc tggaaagggt
     2941 aaaggaagca ggtgatgttg cattaatcaa atattctaat gaattcgata aagcaaactt
     3001 ttcctcccca aaggattttg tggtctcaaa agaggagata gaagaatcta tagatgtcgt
     3061 tggagaagaa tttatatccg cagttaatat ggcaatcaaa aatatcaggg atttttattc
     3121 cagaatgcca agacctcaaa actggttcta tctgaaaaat gggaaactct ccgggcagat
     3181 ggtagtacct ttagagagcg tcgggatata tgtccctggt gggagggctc catatccatc
     3241 cactgtgatg atggctgcta tcccagcaca gattgccggg gtaaagagga tagtggttac
     3301 caccccaaaa cctgtgccag aagtactagc ggtatgcaag ctattggata tatatgaggt
     3361 atacagactc gggggtgcac atgcaattgc aggtctagca tatggaaccg atacaattac
     3421 aagagtcgat aagattgtcg gtcccgggaa tagattcgtg gtatctgcca agaaatatgt
     3481 atttggagaa actggtatag agatgattcc aggaccaagc gagatagttg ttctggcaga
     3541 tagtaacgct aacccaaaat ttgtggctat cgacctatta tctcaggctg agcatgaccc
     3601 gttagccatg tctatactgg ttacagactc agaggatcta gctaatagag ttatagagga
     3661 gatgggaata cttataagag aactctcaac ctgtgagacg gctaaggaat catgggaatc
     3721 ctggggagga atagttctgg tcaactctat agatgaaggt atagatacag taaattttat
     3781 agccccagaa catctcgaat tacagattaa aaaccctgca tcttatctgg aaaagataag
     3841 aaatgccggg acaatattta tcggagaaaa aacccctgaa gctattggtg actatatagc
     3901 tggttcaagt catatcttac caacaggtgg ttctgcaaga ttcagctcag gactgaatgt
     3961 cctggacttt ttaaaattaa taaatataac acagtacaac gagattacag aagactttaa
     4021 gtctggcagt ataatagctg atagagaagg attctcggcg cacaagaaat ctttagagat
     4081 aagagatagg tgatataaaa tcagcaagga aagagttgtt gtaggtctaa gtggaggggt
     4141 tgactcctcc gtctctgcca ttctattaaa gatggcagga tacgatgtga taggggtaac
     4201 tatgagaata tgggggggag aacctactaa aactggcaaa agacatagct gttatggacc
     4261 agaggaacta gatgatatag aagatgccag gagggtctgc gagagacttg atataccctt
     4321 ctatgagata gatctgataa aagaatacag ggagatagtc ctgacttact ttaaaaatga
     4381 gtatctaaag ggaaggacac caaacccctg tataagatgc aatcaattgc taaaattcaa
     4441 agccctcctg gaaaaggtag aaaagattgg tatagaattt gactatttcg ctaccggaca
     4501 ctatgcaaga atagaatatg attctgatag agacaggtat attctgaaaa aagggattga
     4561 cgagagaaga gatcagtctt attttctctt cagtctcaca cagaatc
//
LOCUS       DAOTKU010000078         4594 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_16264_length_4594_cov_3.478299, whole genome shotgun sequence.
ACCESSION   DAOTKU010000078 DAOTKU010000000
VERSION     DAOTKU010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4594
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16264_length_4594_cov_3.478299"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1155)
                     /locus_tag="P5310_02820"
     CDS             complement(<1..1155)
                     /locus_tag="P5310_02820"
                     /inference="COORDINATES: protein motif:HMM:NF014269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HRU32330.1"
                     /translation="MPRKKNDILPVLPLKNMVVFPSTIVPVYVGRDPSVRAIELSSEY
                     DRNIFVLTQKDNELDDINPSDLYDIGTVGEIIQTIKLPDGSYRAVIEAKYRAKVLECV
                     YSETLSAFIAKVEKLEDIREDSLELEALSRIVLSKFDEYIRLNKDIPEEASINLEEVR
                     DEPDKLVDIIASNLPIRSSVKQDLLSVVSPKERLLKLAVILERELKILRIEEDIQVKV
                     QREVEETQREYYLRAKLKVIQEELGEKEDSSGASFYKDKIKLLGLSEDTEDKLMEEAD
                     RLNKVPPFSPESGVIKTYLDWIIGLPWGVYTKDKLNLKDVRATLDKNHYGLQEVKERI
                     IEYLAVKKYGGTRKSPILCFVGPPGVGKTSLAQSISTALGRNFVRISLGGI"
     gene            complement(1148..2407)
                     /gene="clpX"
                     /locus_tag="P5310_02825"
     CDS             complement(1148..2407)
                     /gene="clpX"
                     /locus_tag="P5310_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HRU32331.1"
                     /translation="MKTRDTLRCSFCGKSQDEVKKLIAGPGVYICDQCVRICAEILDE
                     EDISPKSTPDKLTWDKIPKPHEIYDKLNEYVIGQEKTKKILSVAVYNHYKRILAPSDD
                     KDDVEIGKSNILLIGPTGSGKTLMAQVLAKTLDVPFTIADATTLTEAGYVGEDVENIL
                     LRLIQVANYDIKRAEKGIVYIDEIDKIGRKSENPSITRDVSGEGVQQALLKILEGTIA
                     NVPPQGGRKHPHQEFIQIDTTNILFICGGSFEGLEKIVASRIKKKSIGFTGELVSGED
                     VYDVLSYVLPEDLIKFGLIPELVGRLPVVSTLQSLTREELMRILIEPKDAILRQYQRL
                     LALDGVELIFTPDVLSAVAEEAIKQGTGARGLRSIIEKIMLDVMYEVPKRPDIVRCVI
                     DASVIRGEGNPVLLTQKEVERLARESA"
     gene            complement(2417..3013)
                     /gene="clpP"
                     /locus_tag="P5310_02830"
     CDS             complement(2417..3013)
                     /gene="clpP"
                     /locus_tag="P5310_02830"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013536896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HRU32332.1"
                     /translation="MGDYLVPIVIEQTSRGERAYDIYSRLLKERIIFIGGPIEDEIAN
                     LVMAQLLFLEGESPEKDIYIYINTPGGSITAGMAIYDTMQYIKPEISTICIGQAASMG
                     AVLLAAGAKGKRYALPHSRIMIHQPAIYGGLQGPATDIDIQTKELLRLKRIVNEILAK
                     HTSQPLEKVERDTDRDFFMSAQEAKEYGIIDEIIEKRI"
     gene            complement(3013..4230)
                     /gene="tig"
                     /locus_tag="P5310_02835"
     CDS             complement(3013..4230)
                     /gene="tig"
                     /locus_tag="P5310_02835"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HRU32333.1"
                     /translation="MEKEIVHNGSRVQGKISYDQSEVTKAIGEELRLRASSVVVPGFR
                     KGKAPLKLVENFIGRETIVDRAIDRLVNTGIEDFIKEYEIVPLEIRDIDIVERGEGKP
                     LVFTFEIDTYPRVEVENYRELISDLVWEEPVVTEEDVDNFIENIRKRMGVWRDVEDRK
                     IVENGDYVLIDIKDDPSKLDESIETGSIIKIGEGLISPGDDESLVGKGVGESVEIKTR
                     FPEDYREESLRGREITLYVLIKGIKILELPPVDEEFARKFNYQTVEEMREGFKKSIYR
                     ERLAQKEQELSQSIRDRLAQAVTVEIPEIMVRDQLESWKRMLYQNREAPEDFDEKNYD
                     YASDHIKSTLALRYIAEKESIEPTEEEVNRIISSWKVKNITEDVRRSANSIAKEDKTL
                     NFLIELIKGERTK"
     gene            complement(4298..4372)
                     /locus_tag="P5310_02840"
     tRNA            complement(4298..4372)
                     /locus_tag="P5310_02840"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4338..4340),aa:Gly,seq:gcc)
     gene            complement(4385..4470)
                     /locus_tag="P5310_02845"
     tRNA            complement(4385..4470)
                     /locus_tag="P5310_02845"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4434..4436),aa:Leu,seq:tag)
     gene            complement(4480..4555)
                     /locus_tag="P5310_02850"
     tRNA            complement(4480..4555)
                     /locus_tag="P5310_02850"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4520..4522),aa:Lys,seq:ctt)
BASE COUNT         1213 a         1171 c          708 g         1502 t
ORIGIN      
        1 tatacctcca agcgaaatcc tgacaaaatt tctgccaaga gcagtactta tggattgagc
       61 cagagatgtt ttccctactc caggaggacc aacaaaacac aatataggac tcttcctggt
      121 cccaccatat ttcttaacag ccaaatattc tattatcctt tcctttacct cctggagacc
      181 atagtggttc ttatcaagag tagcccttac atcctttaga ttaagtttat ccttagtata
      241 gacaccccac ggtaagccaa taatccagtc cagataggtt tttattacgc cactctctgg
      301 agaaaaagga ggaactttat ttaatctatc tgcctcttcc atgagtttat cttcagtatc
      361 ttctgataaa cctaaaagtt taatcttgtc cttatagaaa gatgcaccag aggaatcttc
      421 tttctcgcct aactcctcct gtataacctt caatttagcc ctgagataat attcccgctg
      481 agtctcctct acctctctct ggaccttaac ctgtatatct tcctctattc tgagtatttt
      541 aagttctctc tccaatataa cggcaagctt gagtaacctc tctttagggg agactacaga
      601 aagcaggtcc tgttttacag aacttctaat tggaagatta gaagcaataa tatctacgag
      661 tttatccggt tcatccctta cctcttcaag atttatagat gcctcttcag gaatatcctt
      721 attcagtctt atatattcat caaattttga taaaactatc ctggagagtg cttccagttc
      781 cagtgagtct tccctgatat cttctaattt ctccacctta gcgataaagg cagagagtgt
      841 ctctgaatag acacattcaa ggaccttagc cctatattta gcctctatca ccgctctata
      901 acttccatcc ggtaacttta tcgtctgtat tatctctcca actgtcccta tatcatacag
      961 gtctgaagga tttatgtcat ccagttcgtt atccttctgt gttaacacaa agatatttct
     1021 atcatattca ctggataact ctatagccct gacagaagga tccctcccga catagacagg
     1081 gactatagtg gatggaaaga caaccatatt ctttaagggt aataccggta atatatcatt
     1141 cttcttccta ggcactttct ctagccaacc tttctacttc cttctgggtc aaaagcacgg
     1201 gattgccttc tcctcttata acacttgcat ctattacgca tctaacgata tctggtcttt
     1261 tagggacctc atacatcaca tccagcataa tcttttctat tatgcttcta agacctctgg
     1321 caccagtccc ctgttttata gcctcctcag ctacggcgga aagaacatcc ggggtaaaga
     1381 tcagttctac cccatccaat gccagtaatc tctgatactg tctcagtata gcatctttag
     1441 gttcaatcag tatcctcatc agttcttccc tagtcaaact ctggagcgtt gaaaccaccg
     1501 gtaatcttcc tacaagttca gggataagtc caaatttaat gaggtcttct ggtaatacgt
     1561 atgacagcac atcataaaca tcctcaccag ataccagttc acctgtaaac cctatagact
     1621 tcttcttaat tctggaagct actatcttct ccaatccttc aaaagaacca ccacatataa
     1681 acagaatatt agtggtatct atctgaatga attcctgatg gggatgtttt cttccacctt
     1741 gagggggaac gttggctatt gtcccctcca gtatctttaa gagcgcctgc tgaacccctt
     1801 caccggaaac atctctggta atagaagggt tctcagattt gcgtccaatc ttatcgatct
     1861 cgtctatata tactattccc ttctctgccc tctttatatc ataattagca acctggataa
     1921 gtctgagaag tatattctct acatcttctc cgacgtaacc agcctctgtc agtgttgtag
     1981 catctgctat ggtaaacggg acatcaagcg tcttagccag aacctgagcc ataagtgttt
     2041 tacctgaacc ggtgggtcct atcagaagta tattactctt acctatctcc acatcatcct
     2101 tatcatcgga cggagctaat atcctcttat agtggttata aactgcaaca gagaggatct
     2161 tcttggtctt ttcctgtccg ataacatact cattaagttt atcgtatatc tcatgtggct
     2221 tgggtatctt atcccaggtc agtttatctg gggtactttt aggcgatata tcctcttcat
     2281 ccaatatctc tgcgcatatc cttacacact gatcacagat gtaaacacca ggaccagcta
     2341 taagtttctt cacctcatcc tgactcttac cacaaaatga acatctaaga gtatccctgg
     2401 tcttcatata tcacctctaa attctctttt ctattatttc atctattatt ccatactcct
     2461 tagcttcctg agcactcata aaaaagtctc tatcggtatc tctctccacc ttttccagag
     2521 gctgagaggt atgtttggca agtatttcat tcactattct cttcaatctc aggagttctt
     2581 ttgtctgaat atctatatct gttgccggac cctgtaaacc accgtaaatt gctggctgat
     2641 gaatcattat cctgctgtga ggaagggcat atctcttacc cttagcgcca gcagccagaa
     2701 gaacagcacc catactggca gcttgaccta tgcatatagt acttatctct ggctttatat
     2761 actgcatcgt atcatagata gccatcccag cagtgattga tccaccgggc gtatttatgt
     2821 atatatagat atccttctca ggactttctc cttctaagaa taaaagttga gccattacca
     2881 gattggcaat ctcatcctct atcggaccac caataaagat aatcctctct tttaagagcc
     2941 tggagtatat atcataggct ctttctcctc tacttgtctg ttctattact attggaacaa
     3001 gatagtctcc catcactttg ttctctctcc ttttattaat tctatcaaaa aattcaaggt
     3061 cttatcttcc ttagctatac tgttagcact acgcctgaca tcctcagtaa tatttttaac
     3121 tttccaggaa ctgataatcc tatttacctc ctcttcggta ggttcgatag actctttctc
     3181 cgctatataa cggagagcca gggtagactt tatatgatct gaggcataat catagttctt
     3241 ttcatcaaaa tcttctggcg cttctctgtt ctgatatagc atcctcttcc agctttcaag
     3301 ctgatctctt accattatct ccggaatctc cacagtaact gcctgagcaa gacgatctct
     3361 tatagactgt gacagttcct gttctttctg ggccagtctc tccctatata tgctcttttt
     3421 gaatccttcc ctcatctcct ctaccgtctg atagttaaat ttcctggcaa actcctcgtc
     3481 tactggaggt agctctaata ttttaatccc ctttataagg acatacagag ttatttctct
     3541 acctcttaaa gattcctccc tgtaatcctc aggaaatctg gtctttatct ctaccgattc
     3601 tccaacccct ttcccaacaa gagactcatc atctccaggt gagatgagcc cttctccaat
     3661 ctttataata gagcctgttt ctatactttc atcaagcttt gatgggtcgt ctttaatatc
     3721 tatcaggacg taatccccat tctctacaat ctttctatcc tctacatccc tccatactcc
     3781 catcctcttc cttatattct ctataaaatt atccacatct tcttcagtaa caacaggctc
     3841 ttcccaaaca aggtcagaga tcaattctct ataattttcg acctctactc tgggataagt
     3901 gtctatctca aatgtaaaaa caagaggttt cccctctcct ctctccacta tatcaatatc
     3961 tctaatctcc agagggacta tctcatactc ttttataaaa tcctcaattc cagtatttac
     4021 aagtctatca attgctctat ctacaatagt ctccctgccg ataaagtttt ctactaactt
     4081 gagaggagcc ttaccttttc tgaagccagg gaccacaacg ctggatgctc ttaatcttaa
     4141 ttcttcccct atagcttttg ttacttcgga ttgatcgtat gatatctttc cttgaaccct
     4201 actgccatta tgaacaattt ctttttccat caatacctcc ataaatatta ataaaaaata
     4261 actcgatagc atacgctatc gagaataatc ttctatatgg agcggaagac gggatttgaa
     4321 cccgcgaccc tctccttggc aaggagatgc tctaccgctg agccacttcc gcatctctat
     4381 ctactggtgc gaggagcggg atttgaaccc gcatgggtgt taaccactag atcctaagtc
     4441 tagcgcgtct gccagttccg ccaccctcgc tctactaagt ggtgggtcgt gcagggctcg
     4501 aacccgcgac agcctgatta agagtcaggt gctctaccaa ctgagctaac gacccgtaac
     4561 atatattatt ttatcaaaaa attttaatat tgca
//
LOCUS       DAOTKU010000079         4587 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_16288_length_4587_cov_3.331862, whole genome shotgun sequence.
ACCESSION   DAOTKU010000079 DAOTKU010000000
VERSION     DAOTKU010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4587
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16288_length_4587_cov_3.331862"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(316..747)
                     /locus_tag="P5310_02855"
     CDS             complement(316..747)
                     /locus_tag="P5310_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015792064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1284 domain-containing protein"
                     /protein_id="HRU32334.1"
                     /translation="MLIEGKGKEMANILKIRAHHLLCIQGFQGYGYSEDFVKNMSKII
                     KSMDLNPEVKIITECDIICSCCPHNVEGVCQKGPDSAQEVRNMDTRILEKLHIEDGMI
                     GRVKDVLSLINTELRNVSDIQDICGDCAWKERCLWFISRDK"
     gene            1145..1723
                     /locus_tag="P5310_02860"
     CDS             1145..1723
                     /locus_tag="P5310_02860"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HRU32335.1"
                     /translation="MKAYRLSYKIFVIILLLLSLIGCLLVFTPREVTEEQALESLLVA
                     LEQEGKPYSFGEQSPHAGFDCSGLVIWAYQQVIPGIRFMVNGELETDAMVEELYDNNI
                     RKLNIEEIKPGDLIFLIRDGELTYPTHMGLFVRWIDKTKFEIIHASSYWGKVIIEVWS
                     LNEVIRGHRFYCAGRLQYYSYDIVNGGCYYSQ"
     gene            1868..2545
                     /locus_tag="P5310_02865"
     CDS             1868..2545
                     /locus_tag="P5310_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32336.1"
                     /translation="MEKKLYRVVSRIDGFPYGGRYFDGIEVVWFYSERREMPPISYNE
                     VIENYKERREKEDINKIESYIDELFVSYEASRLKKYLDQHFKDTVTEIREVDSFPDNS
                     DVIPLKDVPIGGGIDFYLLWKEDKYPLSFKVEGIFDIRPVWRKTAQKQWKIDLTLNDL
                     DLYELPSLTIGDRSIYVLDSGKIGGDFLVVTLNEETEEFLKSKGFRLEEIPGKIYKLY
                     FQQDDKT"
     gene            2532..4370
                     /locus_tag="P5310_02870"
     CDS             2532..4370
                     /locus_tag="P5310_02870"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HRU32337.1"
                     /translation="MIRHNSTARTILIVVLFILIIFPGYARADYFLSGGFFSSEANIR
                     KLPLEVSSIAFGPEDRLCLLDKREGKIQIYSSKGLLIREFKSNADAITIDPWGYLYTV
                     TDGINVEVFDKDGNSVRKILLESKEPRYEFISYSGFLSVDERGYIYIVSASSGKVSVY
                     SPSGVYAGDLIKSGYGASDLTMVSAVTIDSKGNIYVAGNLPRITANRFEPNLVVVKKL
                     SYQGYADTVFGPLTNIFVGGMVVDDLGNLYLLDTSSCNIYKFDKRGAMVTSFFAGRGA
                     SCLAIRSDGYLAIGYGGEIRLFHPSRLMNFIDKANKALLDGEYDTAINYWNEALRLNN
                     YLKFIHSGLGETYLYMKDYRNALGEFKLAEDRTRYSSTIVLYRKEIFYRYILLWGILF
                     LVILNAIILWGKKLFFLFNNIIGRMLYSPVRALAEVKKRDILFGVILIVSLALIDALN
                     RRFVNYIFQPTLEDINYIFLRRLLMFSGLWLVSGTVFYGIGEIFDGMGTWKQCMLTTV
                     MCFVPYIIFSLPLSLISNLLTFQEKVYYDYAQQGLMLWCVILFFVNVYTTQQLSAGKN
                     LLIFSLTAIGLVFSISIILFLQGVNREIWAFIKDVYLEISYRLVGF"
     gene            4370..>4587
                     /locus_tag="P5310_02875"
     CDS             4370..>4587
                     /locus_tag="P5310_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32338.1"
                     /translation="MSRCLFPILLLMLFITPLAYAGLDISYSAEKDLLTVRDDGYTMM
                     SSPPDWKSVNDYWDSRIRSMFIVEYWDRG"
BASE COUNT         1386 a          789 c          918 g         1494 t
ORIGIN      
        1 aaaacctttc catttttact gtaaaaagga taaatggcgt tcgaaatcat aaaatcgatt
       61 agggatgtat cacgtatctc ttcattatcc ttcaagctac aatccttcat ctagcaagca
      121 acctcctttt agtctctaaa atggcattac gggggttttc cagcgaaaat gccttattca
      181 taagattcgc cccatctaat tcttctcctg ttttgtcttt cactatttcc ttaacacgta
      241 tattttttgt gagtcaaagt ccattatctt ttaacatcac taataatata tagcaaagaa
      301 aagcaaaaat cacttctatt tatctcgtga aataaaccag aggcatctct ccttccaggc
      361 acagtcacca catatatcct ggatatcaga aacatttctg agctctgtat ttataagaga
      421 aaggacatct ttgaccctgc ctatcatccc atcttctata tgtaattttt ccaaaattcg
      481 cgtgtccata tttctaacct cttgagcaga atcaggacct ttttgacata ccccttctac
      541 attatgagga caacaagagc agatgatgtc acattcagta attatcttta cttcagggtt
      601 taaatccata ctcttaataa ttttactcat attctttaca aaatcttcac tataaccgta
      661 tccttgaaaa ccctgtatac ataagaggtg gtgagctcta atctttaaaa tatttgccat
      721 ttctttacct ttaccctcta tcagcatcaa cttcactgct gtaagcctcc ttgggggtac
      781 aagttatatt ttttaagtct ctttattatc ccaagcgttc tgaaatttat agcaaagatt
      841 ttctctgtca tatcccaatt gtaatggatt attgataaca tattccagga taagattcaa
      901 ctcatcttat tcctggcaat gacatttttc tttgtatcat ttttatattc cgcaggggca
      961 tgcccctaca atttcgtctt ttatgataat aatcctcaga aattacgata aaattatcca
     1021 attccacatt cgaataatac cagcattgtt ttgttgacgt tctataatct gatataatga
     1081 atactgtgat cagcgttgag atttaaggaa gcaaagaaga atactgttaa taacctgttg
     1141 ttttatgaag gcttatagat tatcatataa gatctttgtt attattttac tcttgctctc
     1201 tttaattggt tgccttcttg tatttactcc aagagaagtt actgaagagc aagccttgga
     1261 atctcttctt gttgcgctag aacaggaggg aaagccatac tcatttggag aacaatctcc
     1321 tcatgcgggt ttcgactgtt caggacttgt tatatgggca taccagcagg ttattccagg
     1381 aataagattt atggtcaacg gtgagttaga aacagacgca atggttgagg agctatatga
     1441 taataatata agaaaactaa acatagaaga aataaaacca ggtgatctga tctttctcat
     1501 aagagatgga gagcttacat accctacaca tatgggactc ttcgttagat ggatagataa
     1561 aacaaaattt gagattatac atgcttcctc ttactgggga aaagttatta tagaagtctg
     1621 gagtttaaac gaagttataa gagggcatag gttttattgc gctggaagat tacaatatta
     1681 tagctatgat attgtaaatg gtggatgtta ttatagccag tgaagtactt atctcacgtc
     1741 ccatattcca aaaatgacat ggtaacattc ctatctcgaa tttcagttac tttctattgt
     1801 ttaatttaaa tttctgtttt atacttatct aagacaatta aagaggaaag gagttgaaga
     1861 aggcttggtg gagaaaaagc tctacagagt tgtctccagg atagatggtt tcccatacgg
     1921 gggaagatac ttcgatggta tagaagtggt atggttttac tctgagagac gagagatgcc
     1981 cccaatcagc tataatgaag ttatagaaaa ctataaagag agaagggaaa aagaagatat
     2041 aaataagatt gaaagttaca tagatgagct atttgtttct tatgaggctt ccaggttgaa
     2101 aaaatatctg gatcagcatt ttaaagatac tgtaacagaa ataagagagg tagattcatt
     2161 ccctgataat tcagatgtta ttccattaaa agatgtcccc attggaggag gtattgattt
     2221 ctatctcctc tggaaagagg ataagtaccc gctatctttt aaggttgagg gaatctttga
     2281 cataagaccg gtatggagaa agacagcaca gaaacaatgg aagatagatt taacgttgaa
     2341 tgatttagac ctgtatgagc tcccatccct taccataggt gacaggagta tctatgtact
     2401 ggattctgga aagatagggg gtgatttttt agtagtaacc ctcaatgagg agacggagga
     2461 gtttttaaag agcaagggtt tcagattaga ggaaatccct gggaaaatat acaagctcta
     2521 tttccagcag gatgataaga cataattcta ccgccaggac aatacttatt gtcgttctat
     2581 tcatactgat aatatttcca ggatatgcaa gggcagatta tttcctcagt ggtggtttct
     2641 tttcttcaga agccaacatc agaaaacttc ccctggaggt gtcatctata gccttcggtc
     2701 cggaagatag gctttgtcta ctggataaga gagaggggaa gatacagatt tactcgtcga
     2761 agggtctctt gataagagag tttaaatcta atgcagacgc tattactata gacccctggg
     2821 gctatttata taccgttaca gatggtataa acgtagaggt atttgacaaa gatggtaatt
     2881 ctgtaagaaa aatccttctg gaatccaaag aaccaaggta tgaatttatc tcttactcag
     2941 ggtttctctc tgtagatgag aggggttaca tatacattgt cagtgccagc tctggtaaag
     3001 tctctgtata ttctccttct ggagtttatg cgggagacct tataaagagt ggatatggag
     3061 catctgatct tacaatggtc agtgctgtta ctattgattc caagggtaac atatacgtag
     3121 ctgggaatct tcccaggatt acagcaaata gatttgagcc gaatctggtt gttgtaaaaa
     3181 agctcagtta tcagggttat gcagacacag tctttggtcc acttaccaat atatttgttg
     3241 gtggtatggt ggtggatgat cttggtaatc tataccttct cgatacatct tcctgtaata
     3301 tatacaagtt tgataaaagg ggggcaatgg ttaccagctt ctttgccggt agaggtgcca
     3361 gctgtttagc cattcgaagt gatggatatc tggcgatagg ttatggtgga gagattagac
     3421 tctttcatcc ttccagattg atgaacttca tagataaggc aaacaaggca ttacttgatg
     3481 gagaatatga tacagctata aattactgga acgaagccct gagattaaat aactatttga
     3541 agtttataca ttctggactg ggtgagacgt atctttatat gaaggattac aggaatgctc
     3601 tgggagaatt taagctagca gaagacagga ccagatattc cagtacaatc gtactttata
     3661 gaaaagaaat tttctacagg tatattctcc tctggggtat actcttttta gtaattctaa
     3721 atgccattat cctgtggggg aaaaaattgt tcttcttgtt taataatata ataggaagaa
     3781 tgctctattc ccctgttagg gctttagctg aggtaaagaa gagggatata cttttcggtg
     3841 tcattctaat cgtctctctg gcacttatag acgcattgaa tagaaggttt gtaaattata
     3901 tattccagcc tacccttgaa gacataaact atatattcct ccgtaggctc cttatgtttt
     3961 ccgggctatg gttggtgagt gggacagttt tctatggtat tggagagata tttgatggaa
     4021 tgggtacatg gaaacagtgt atgttaacta cagttatgtg ttttgtaccg tatataattt
     4081 tttctcttcc cctatcattg atcagtaatc ttctcacatt ccaggagaag gtatattatg
     4141 attatgccca gcaaggactg atgctctggt gcgttatttt attctttgta aatgtatata
     4201 ctacccagca actctctgct ggaaaaaatc tgttgatctt ttctctaacg gcgatcggtc
     4261 tggtgttttc catctccata atcttattcc ttcagggcgt aaacagagag atatgggcat
     4321 ttatcaagga tgtatacctt gaaataagtt atagattggt gggattctga tgtctaggtg
     4381 tctgtttcca attctgctgt tgatgctttt tataactcca ctggcgtatg cagggctgga
     4441 catatcttac agtgcagaaa aagacctgct taccgtcaga gatgacggat ataccatgat
     4501 gagttctcct ccagactgga aatctgtaaa cgattattgg gattccagga taaggtcaat
     4561 gtttatagtt gaatactggg atagggg
//
LOCUS       DAOTKU010000080         4581 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_16318_length_4581_cov_2.730446, whole genome shotgun sequence.
ACCESSION   DAOTKU010000080 DAOTKU010000000
VERSION     DAOTKU010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4581
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16318_length_4581_cov_2.730446"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1511)
                     /gene="ilvB"
                     /locus_tag="P5310_02880"
     CDS             complement(<1..1511)
                     /gene="ilvB"
                     /locus_tag="P5310_02880"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HRU32339.1"
                     /translation="MKLTGAQIVIEALKREGVDVIFGIPGGAIIDVYDALYDSGIRHI
                     LAHHEQGAAHMADGYARATGKVGVCFATSGPGATNLTTGIANAYMDSVPIVAITGQVA
                     TGLIGKDAFQEADTVGITSPITKHNYLIRDVNELAKTIKEAFYIAKTGRPGPVLIDIP
                     KDIQIATADFDYSMEVDIRGYKPSVTGHIKQIKEAAKLINESKKPCILAGGGVIISGA
                     SEELFKLATKANIPVATTFMGIGSFPQDHPFSLKSAGMHGNYAANYALTECDLLIAIG
                     CRFSDRTTGKADEFATRAKKIHIDIDPAEINKNVVVDIPIVGDAKIILNSLLEYVEEK
                     REEEWLDYIMGLKEEHRLTYADGPAVKPQFVIEKICEMVSENTVIVTEVGQNQMWSAL
                     FFTHKKPRHFISSGGLGTMGFGFPASIGAKVGRPDALVIDIAGDGSFMMNIQEIATAV
                     ENNIPVKVIVLNNRALGMVRQWQELFYKKRYSSTILKEVNFAEIAKSFGAKGI"
     gene            complement(1508..3172)
                     /gene="ilvD"
                     /locus_tag="P5310_02885"
     CDS             complement(1508..3172)
                     /gene="ilvD"
                     /locus_tag="P5310_02885"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HRU32340.1"
                     /translation="MRSDAIKKGIERAPHRSLLKALGIDDVELERPFIGVINSYSEII
                     PGHAHLDRVVEAVKAGIMEAGGVPFECETMAICDGIAMNHSGMGFSLPSRELIADSVE
                     SLAEAYKFDGLVVIPNCDKIVPGMIMGLARVNIPAILVSGGPMMPGEVDGKRADLNTI
                     FEAVGAVVSGKKDMEYLRELEYLVCPGCGSCAGMFTANSMNCLSEAVGIALPGNGTIP
                     AVEAERIRLAKSAGREIIRLVREGITPDMILTRSAFLNGIALDMALGCSTNTVLHLTA
                     IANELGMGLDFDEINSIADKTPYLCHLSPVGPYYVVDLNKAGGVMAVLKELNKKGLID
                     RGTLTVSGKKLGELIDSAVVKDYNVIRPVDNPVRSTGGLSILRGNLAPDGAVVKKSGV
                     AESLYRFVGKAKVFDGEEEASRAIFDRKIEKGDVVVIRYEGPKGGPGMKEMLTPTSTL
                     VGMGLDLDVALITDGRFSGATRGLAIGHISPEAWEGGPIAYVQDGDPIEIDLNKREVN
                     LLVDREELSRRTYTLPETHKKKGYLGRYQRLVSSASYGAVMEVNRR"
     misc_binding    complement(3228..3389)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(3525..3893)
                     /locus_tag="P5310_02890"
     CDS             complement(3525..3893)
                     /locus_tag="P5310_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004067167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HRU32341.1"
                     /translation="MDANVFLEILLRQTKKEKCKKFLENNTGSLNITDFSLHSIGVIL
                     FKYSKEDVFQRFVEDVIPNVKLLSLPSEIYGEIVNAKRSFNLDFDDAYQYATAKYYGL
                     KLVTMDKDFEMIREIEVLFL"
     gene            complement(3902..4114)
                     /locus_tag="P5310_02895"
     CDS             complement(3902..4114)
                     /locus_tag="P5310_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2281 domain-containing protein"
                     /protein_id="HRU32342.1"
                     /translation="MYEKELEQKIQQLDEDLKREVLDYVEFLLKKYKGEETKKKKFNF
                     DWEGGLSEIRDEFTSVELQHKALEWR"
     gene            complement(4175..>4581)
                     /locus_tag="P5310_02900"
     CDS             complement(4175..>4581)
                     /locus_tag="P5310_02900"
                     /inference="COORDINATES: protein motif:HMM:NF013335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HRU32343.1"
                     /translation="AGRVVFDSGIPITMVGLDVTLKCKLGKKSLDKIFDFNSERTNFL
                     SELIRIWQEDDESRFPILHDPLAVATLIDPSLVRRESMHIKVETRGEFTRGVTVVTDT
                     PYGDRKGDSNVDVSVDVDSDRFIQFFLERILK"
BASE COUNT         1244 a         1200 c          706 g         1431 t
ORIGIN      
        1 cttatccctt ttgccccaaa agatttggca atctctgcaa aattaacctc tttcaggatg
       61 gtggatgaat atctcttctt atagaagagc tcctgccact gtctcaccat acccagggct
      121 ctattgttta aaactatcac ctttacaggt atattattct ccactgcggt agcaatctcc
      181 tggatattca tcataaaact tccatctcct gctatatcta ttaccagggc atctggtcta
      241 cctacctttg ccccaattga tgcagggaaa ccaaacccca tagtccccaa gccgccagaa
      301 gagataaagt gtctgggctt cttatgtgta aagaaaaggg cagaccacat ctgattctga
      361 cctacctcgg ttactataac tgtattctcg gacaccattt cacatatctt ctctatcaca
      421 aactgaggtt tgactgcagg tccatctgca taggtaagtc tatgttcctc tttaagcccc
      481 attatataat ccagccactc ttcttctctt ttttcttcca catactctaa gagagagttc
      541 aaaataatct tcgcatctcc aactataggt atatcaacca ccacattttt atttatctcg
      601 gcagggtcta tatctatatg gatttttttt gctctagtgg caaactcatc cgcctttcca
      661 gtagttctat cactgaatct acaaccaata gctatgagaa ggtcacattc tgtaagggca
      721 tagtttgctg catagtttcc atgcatcccg gcagacttta atgagaaagg atggtcttga
      781 gggaaactcc ctatccccat aaaagttgta gccaccggaa tattggcttt tgtagccagt
      841 ttaaagagct cctcactggc tccagagatt ataacccctc ccccagccag gatacatggc
      901 tttttggatt catttattaa tttcgccgcc tcttttatct gttttatatg cccggttaca
      961 gatggtttat accctcttat atctacctcc atagaatagt caaaatctgc agttgcaatc
     1021 tgtatatcct tcggtatatc tatgaggaca ggtccaggtc ttcctgtctt tgctatataa
     1081 aaagcctctt ttatggtctt tgccagctca ttcacatctc ttatcaggta attatgcttt
     1141 gttattggag atgttatgcc aactgtatct gcctcctgga atgcatcttt ccctatcaga
     1201 ccggtggcaa cctgacccgt aatcgcaact atcggtacag aatccatata agcattggct
     1261 atccccgttg tgaggtttgt ggcgccagga ccagaggtag caaaacacac acctaccttt
     1321 ccagtagccc tggcataacc atcagccata tgcgctgccc cctgttcatg atgagcaagt
     1381 atatgcctta tccctgagtc atagagagca tcatatacat ctattattgc tccgccagga
     1441 ataccaaata taacatccac tccctctctc tttaacgctt ctataactat ctgtgcacct
     1501 gtaagtttca tctcctatta acctccatta ccgccccata agaggcagag gatactaatc
     1561 tctgatatct tcctaaatat cctttcttct tatgagtctc aggtaaagta tatgtccttc
     1621 tggaaagttc ttctctatcg acaagaaggt tcacttctct cttatttagg tctatctcta
     1681 tagggtctcc atcctggaca taggctattg gtcctccctc ccatgcctct ggagatatat
     1741 gacctatagc taaacccctg gtagcccctg agaatcttcc atctgtaata agagcaacat
     1801 ccagatctaa ccccatacca acaagtgtag aggttggtgt gagcatctct ttcatccctg
     1861 gaccgccttt tggtccttca tatcttatga caactacatc tcctttttct atcttacgat
     1921 caaaaatagc cctgctcgct tcctcttcac catcaaagac cttagccttt cctacaaacc
     1981 tatagagaga ttcagccacc ccggatttct tcaccactgc tccatccggg gcaagattgc
     2041 ccctgagtat cgataatccc ccggtactcc ttacagggtt atcaactggt cttatcacat
     2101 tgtaatcctt taccacggcg ctatctatca attcaccaag cttctttcca ctaactgtaa
     2161 gtgtacctct atctatgaga cctttcttat taagctcttt taagacagcc ataactccgc
     2221 cagccttatt aagatcaacc acataatatg gtcctaccgg gctcagatga cagagatacg
     2281 gtgtcttatc cgctatactg tttatctcat caaaatccaa acccataccc aattcattgg
     2341 ctatagcggt aagatgaagt accgtattgg ttgaacatcc cagagccata tccagagcta
     2401 ttccatttaa aaatgcagac ctggtaagta tcatatccgg ggttataccc tctcttacca
     2461 atcttattat ctcccttcct gctgacttgg ccagtcttat cctttcagcc tcaacagcag
     2521 gtattgtccc attgcctggg agagctatac caacagcctc ggagaggcaa ttcatggagt
     2581 ttgccgtaaa cataccggca caggaaccac agccaggaca gacaagatac tccaattctc
     2641 tgaggtactc catatctttc tttccactga cgaccgctcc aacagcttca aatatggtgt
     2701 tcagatctgc tctttttcca tcaacttctc caggcatcat aggtccacca ctgactaaaa
     2761 tagcagggat atttaccctt gctaaaccca taatcatacc tggcacaatc ttatcacagt
     2821 ttggaataac cacaagacca tcaaatttgt aagcctcggc aagagattcg acactatcgg
     2881 cgatgagctc tctggagggg agagaaaaac ccataccact gtgattcatg gctattccat
     2941 cacatatagc catggtctca cattcaaacg gaaccccgcc tgcctccatt atgcctgcct
     3001 ttaccgcctc aactaccctg tcaagatgtg catgtccagg tataatctct gaataactgt
     3061 ttatcacccc tataaatggt ctttcaagct ctacatcatc tatccccagt gcctttaaaa
     3121 gcgacctgtg tggtgctctt tcaataccct tttttatagc atcgctacgc ataatatcaa
     3181 ctccttttca ggttataaaa aagctttccc tccatagcaa atcatctgct agggacggaa
     3241 agcctcccgc ggtaccaccc taattggctt tttgagccca ctctttattc tgtaacggga
     3301 attcccggga taaactacta ttgtttcatc tatccagctc caaggcgagt tcaccttaaa
     3361 cggctactac tcctcttcac agccatttct atattcaatt gtttagagat aatatcagaa
     3421 gaatttttgt aagtcaagta aaatctgact gagtaattct actgaaccat atatccaccg
     3481 gaacaaggtg atatacacca acaccctcta cgatttctat tttattatag gaataagact
     3541 tcaatctctc ttatcatctc aaaatccttg tccattgtga ccagtttcaa gccataatat
     3601 tttgcagtag catattggta agcatcatcg aagtccaaat taaagcttct cttagcattc
     3661 acaatctccc catatatctc cgacggtaag gagaggagtt taacattagg tataacatct
     3721 tcaacaaatc tctggaaaac atcctcttta ctgtatttaa atagaattac tccgatagaa
     3781 tgcaaagaaa aatctgttat attcaggctt ccagtattgt tctctagaaa cttcttgcac
     3841 ttttctttct ttgtctgtcg caagagaatt tctaagaata catttgcatc catcaaaaac
     3901 attatctcca ctccaaagct ttatgttgta gctcaacaga ggtaaattcg tctcttatct
     3961 ctgataagcc accttcccaa tcaaaattaa acttcttttt tttagtttct tcgcctttat
     4021 acttcttcag taaaaactct acataatcta aaacttctct ttttaaatct tcatcaagtt
     4081 gctgtatttt ttgctccaat tccttttcat acatgatact cacccccatc tggacaatat
     4141 tctaatataa taataacctt tttacctata gcttttattt caatatcctc tccaggaaaa
     4201 attgaataaa cctgtcacta tctacatcca cagagacatc cacgttggag tctccctttc
     4261 tatctccgta tggtgtatct gttaccactg ttactcctct ggtgaactct cctctggttt
     4321 ccacttttat atgcatactt tctcttctta ccagagaagg atctattaag gtcgccactg
     4381 ccagcggatc atgcaaaata ggaaacctgc tctcatcatc ttcctgccat atcctgataa
     4441 gttcggagag aaaatttgtc ctctcactgt taaagtcaaa aatcttatca agcgatttct
     4501 tacccagctt acacttaagc gtaacatcca gccctaccat agttatagga attccagagt
     4561 caaataccac ccttcccgcc t
//
LOCUS       DAOTKU010000081         4547 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_16473_length_4547_cov_3.632903, whole genome shotgun sequence.
ACCESSION   DAOTKU010000081 DAOTKU010000000
VERSION     DAOTKU010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4547
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16473_length_4547_cov_3.632903"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..733)
                     /locus_tag="P5310_02905"
     CDS             complement(<1..733)
                     /locus_tag="P5310_02905"
                     /inference="COORDINATES: protein motif:HMM:NF040068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HRU32344.1"
                     /translation="MYRLINSHPYFSCERFFLSQDEIVPLSIESLKPLNEFDVIGISI
                     SYELDYFNVIKILESSHIPVFAKDRDLSYPLIIGGGACMNYNPEPIADIFDLILIGEA
                     EDAIKEILDRYIDMKDAVRSKDDLLFSFLDIEGVYIPEFYKDGKPLVDVAPQRIKRRV
                     FRDFDRETAYSCIYSPDAVFGDMFLIEIERGCPMKCKFCVAGNIYSPCRIKSLDTVKD
                     IILKYGEKYKKIGLMGPLVGGIPYIE"
     gene            complement(899..1936)
                     /gene="ftsZ"
                     /locus_tag="P5310_02910"
     CDS             complement(899..1936)
                     /gene="ftsZ"
                     /locus_tag="P5310_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HRU32345.1"
                     /translation="MSDGNNVTKIKVIGIGGGGVNAVDRIIESNLNGVDFIAINTDLQ
                     ALNKSKAERKIQIGAELTKGLGAGADPNIGRQAAEESKEDIMLAVEGADMVFITAGMG
                     GGTGSGAAPIIAQLSREAKILTVAVVTKPFSFEGRRRMIVAEQAIDELRDKVDALIVI
                     PNDKLLAVSSKSTTIIEAFKMADEVLKAGVQGIVDIIITPGIINPDFADVRTIMQNSG
                     SAIMGIGEASGEQRAVEAASKAISNPLLEDKIDGAKGVLFNVTGGEDLTLTEAYEAAE
                     VIGKSADSDANIIFGAVIDPKMEGKIRITVIATGFENRAKRPVKKSPQGLEGVIINPE
                     ILDIPPFLRGR"
     gene            complement(1952..3181)
                     /gene="ftsA"
                     /locus_tag="P5310_02915"
     CDS             complement(1952..3181)
                     /gene="ftsA"
                     /locus_tag="P5310_02915"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HRU32346.1"
                     /translation="MSKSTLISVLDVGTTKSCVLVSQVDNEGYHIVGYGISPSFGMKK
                     GTVVDIDKVSESVSNALQNAEKICESKIKEISIGVTGEHIYSVVNRGEVEITSENKII
                     SESDVEKVIKRASNYGLPRDKELLHTIPRYYIVDGIRNIDNPVGMRGEKLGVEATLVI
                     GNMSHIQNLVTAIENAGLKVKNIMLQPYASGKAVLTEKETEEGVCLIDIGGGTTDVAV
                     FKDGALYSTFVVPVGGNHITNDLSLVLNLSFDESEKLKIEYGGCDLLKVNRDEMINVN
                     SKGKERQFSKQLVFEIIEARVGELFSYVNALLQKLDLTLMIPSGIVITGGTSLLKDID
                     IVAEREFGIPVRIGYPRDLNNIWETLNSPVYGTALGLLKLEISEISTGKAEPDKGNLF
                     NYLVSRVKRWFQGLFEE"
     gene            complement(3174..3542)
                     /locus_tag="P5310_02920"
     CDS             complement(3174..3542)
                     /locus_tag="P5310_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HRU32347.1"
                     /translation="MIVIVPILALVSGIIIGIFLPVQVTPLYARYIAIVILVALDTIF
                     GGIRTILELKFDATIFLSGFFVNSLLGVILIFIGDRLGVDLYLAAIVAFGVRIFQNLA
                     VIRRYIIKRLSDKIKGEGHE"
     gene            complement(3542..4261)
                     /locus_tag="P5310_02925"
     CDS             complement(3542..4261)
                     /locus_tag="P5310_02925"
                     /inference="COORDINATES: protein motif:HMM:NF017734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HRU32348.1"
                     /translation="MNKRAVWSLAVVCFILGLLSVAQIKFYEAPKYTLFSMRNEQLID
                     MVQRLESQRNELIQEIATLRDQLKQYEESSAKDKTVLEGMLKELNRVKLWAGLLPIKG
                     PGIVVTLDDSKRKIKAGEDLENYIIHDYDLREVVNELWTSGAEAIAINDQRIVVSSEI
                     RCVGSTVLVNGVRLAPPYMVKAIGDADSLINALTMPGGIISALNYISKDFGIIIKIDK
                     KDLLELPPYEGSITLKYGRSE"
     gene            complement(4258..>4547)
                     /locus_tag="P5310_02930"
     CDS             complement(4258..>4547)
                     /locus_tag="P5310_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32349.1"
                     /translation="NCPQTEKIRLLPVLSKEIPTDKIPLIKRIELRDNELYVILYNNI
                     SCRLGEIKDTGKKLELALKTIKSAREKGIKVKEIDVRSLKFPTIKEVKIEQ"
BASE COUNT         1289 a         1131 c          643 g         1484 t
ORIGIN      
        1 cctctatata ggggattcct ccaaccaggg gacccataag accaatcttc ttatacttct
       61 ctccgtattt tagtatgata tcttttacag tatccagaga ttttatcctg cagggagaat
      121 atatattccc tgcaacgcaa aacttacact tcataggaca tcctctctct atctctatca
      181 agaacatgtc tccaaatacc gcgtctggag agtatataca gctataggct gtttctctat
      241 caaaatctct gaatactctt ctctttattc tttggggagc cacatctact aaaggctttc
      301 catccttata aaattcaggt atatatacgc cttctatgtc taaaaaagag aagagcaaat
      361 catccttgga tctgacagca tctttcatat ctatatatct atccagaatc tcctttatag
      421 catcctcagc ctctcctata aggataagat cgaagatatc cgctataggt tcggggttat
      481 aattcataca ggctccaccg cctataatta atggataact taaatcccta tctttagcaa
      541 aaacagggat atgagagctc tctaaaatct ttataacgtt aaaatagtcc agttcataag
      601 atatagaaat tcctataaca tcaaattcat ttaatggttt taaactctct atagatagag
      661 gcacaatttc atcttgagag agaaagaacc tctcgcagga gaagtaggga tgtgaattaa
      721 tgagtctgta caacagatgg attgcaagat tggacatacc cacctcatag gtattggggt
      781 atacaagtcc aaaagaataa tcctttaccc ggctttctat ccctccctct ctctggcgta
      841 ataattctct atctcttcta tattcccaac ttatagacgt aacttgttct ctttccccct
      901 atctacccct taagaaagga gggatatcca ggatttctgg atttattatc accccttcta
      961 acccctgagg actcttcttg acaggtctct tagccctgtt ttcaaaacca gtagctataa
     1021 ctgttatcct gatcttacct tccattttgg gatctataac tgctccaaat attatattag
     1081 catcactgtc ggcgctctta cctataactt ctgctgcctc gtacgcctct gtcaaagtta
     1141 aatcctctcc tccagtaaca ttaaataata cccccttggc tccatcgatc ttatcttcca
     1201 gaagaggatt tgagattgcc ttgctggctg cctctacagc cctctgctct ccagaagcct
     1261 cccctatacc cataattgca gaacctgaat tctgcataat tgtccttaca tcggcaaagt
     1321 caggatttat aatccccgga gtaattatta tatctactat accctgaacc ccagctttga
     1381 ggacttcatc tgccatcttg aaagcttcta ttatggtagt ggacttgcta cttacagcta
     1441 ataatttatc atttggaata acaataagtg catccacttt atccctgagt tcatctatgg
     1501 cctgttcagc aacaatcatc ctccttcttc cttcaaaaga aaatggcttg gtaacaacag
     1561 ctacggtaag gattttagct tcacgtgaaa gctgagcaat gattggagct gcaccgctgc
     1621 ctgtccctcc ccccataccg gcagtaatga ataccatatc cgctccctct accgcaagca
     1681 ttatatcttc tttactttct tcagcagcct gccttcctat gttagggtca gctcctgcac
     1741 ctaaaccttt agtcaattct gcccctatct gaatctttct ctccgctttt gacttgttaa
     1801 gtgcctgtaa atcagtgttt atagcaataa aatctactcc attaagattt gactcaataa
     1861 tcctgtctac tgcatttact cctccccctc caatacctat aacttttatc ttcgtgacat
     1921 tattaccatc tgacatttta tatcttcctc cttattcttc aaataatcct tgaaaccatc
     1981 tcttaaccct agacacaaga taattaaaaa gattaccttt atctggctct gccttcccgg
     2041 tagaaatctc agagatctca agttttagaa gtcctaaagc ggtcccgtat acagggctat
     2101 taagtgtctc ccatatatta ttcaaatctc tgggataacc tatcctcacc ggtattccaa
     2161 attctctttc tgctacaata tctatatctt taagaagaga tgtacccccg gtgattacta
     2221 tacctgaagg aatcatcagg gttaagtcta acttttgtaa tagggcattg acataagaga
     2281 acaactcacc tactcttgcc tctataatct caaaaactaa ctgttttgag aattgtctct
     2341 ccttaccttt agagttcaca ttaatcatct catctctatt cacctttaag aggtcacaac
     2401 ccccatattc gatcttaagc ttctctgact catcaaatga aaggttaaga actaaagaca
     2461 ggtcattcgt aatatgattc cctccgaccg gtaccacaaa agtagaatag agagctccgt
     2521 ctttaaagac cgccacatct gtggtccctc cacctatatc tataaggcac acaccttctt
     2581 ctgtctcttt ctccgtaagg acagccttcc cagatgcgta tggttgtaac attatatttt
     2641 taacctttaa acctgcattc tctatggcgg taaccaggtt ctgtatatgg ctcatattcc
     2701 ctataaccag agtagcttct acacccagtt tttctcccct cattccaaca ggattgtcta
     2761 tatttcttat accgtcaact atataatatc ttggtatggt atgaagcaac tctttatccc
     2821 tgggaagtcc ataattactt gctcttttaa taactttctc tacatctgac tccgagatta
     2881 tcttattttc actggttatc tccacctctc ctctattcac caccgaatat atatgctctc
     2941 ctgttactcc aatacttatc tctttaattt ttgactcaca gatcttctct gcattctgca
     3001 aggcattgga tacggactca gaaaccttat ctatatcaac tacagtaccc ttcttcatcc
     3061 caaaagaagg ggatatccca taacccacta tgtggtatcc ctcattatca acctgagata
     3121 ccagtacgca acttttggta gttcccacgt ccagaacaga tatcagcgta ctcttactca
     3181 tgtccctctc cctttatctt atcagacaat ctttttataa tatacctcct tatgaccgcc
     3241 aaattctgga atatcctcac cccaaaagcg acaatagctg caagataaag atcgactccg
     3301 agtctatcac caataaatat taaaattaca cccagtagag agtttacaaa aaacccggac
     3361 aggaatatcg tggcgtcaaa ctttagctcc aatatggtcc ttattcctcc aaatatagta
     3421 tcaagggcta ccagtatcac tatagctatg taacgggcat acaacggtgt aacctgtact
     3481 ggaaggaaga ttccaattat tatacccgat acaagggcta atatggggac aattactatc
     3541 attactctga cctaccgtat tttaatgtta tactaccctc ataaggaggg agctctaaca
     3601 aatccttttt atctatctta ataattattc caaaatcttt ggatatataa tttaaggcgg
     3661 aaataatacc tccaggcata gtaagcgcat ttataagaga atccgcatct cctatagctt
     3721 tcaccatata aggaggagcc aatcttactc cattaaccag gacggtagac ccaacacatc
     3781 ttatctcact actaaccact atcctctgat catttatggc tatagcttct gccccagatg
     3841 tccagagttc atttacaacc tctcttaaat catagtcgtg aattatgtag ttctccaaat
     3901 cttctccagc ttttatcttc ctcttactat catctaatgt tacaactatt ccaggacctt
     3961 ttattggcag taaacctgcc cataacttta ccctgttaag ttcttttaac atgccctcaa
     4021 gaacggtttt atccttagcc gaagattctt catactgttt aagttggtct ctcagtgtgg
     4081 cgatctcctg tatcagctca ttcctctgag actcaagtct ttgaaccata tcgataagtt
     4141 gctcattcct catactaaat aaagtatatt taggagcttc atagaatttt atctgtgcta
     4201 ctgataataa tccaagtata aaacatacca cggctaaaga ccataccgct cttttattca
     4261 ttgttcaatc tttacctctt ttatggtagg aaacttcaaa ctcctcacat ctatctcttt
     4321 aacttttatc cctttttctc tggcactttt aatcgtcttt aacgctaatt ctaacttctt
     4381 acctgtatct tttatctcac ctagcctgca agatatatta ttataaagta taacataaag
     4441 ttcgttgtcc ctcagctcaa tcctctttat cagaggaatc ttatctgtgg gaatctcctt
     4501 actcagtaca ggcaataatc tgatcttttc cgtctgagga caattta
//
LOCUS       DAOTKU010000082         4507 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_16635_length_4507_cov_3.237421, whole genome shotgun sequence.
ACCESSION   DAOTKU010000082 DAOTKU010000000
VERSION     DAOTKU010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4507
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16635_length_4507_cov_3.237421"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="P5310_02935"
     CDS             complement(<1..639)
                     /locus_tag="P5310_02935"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRU32350.1"
                     /translation="MGNNKSFSFDKIARQYARYRETHPGVLRSLLINISKDSKVLEIG
                     CGTGNYIIMINFISGCQSWGIDISREMLSKAQDRSKSVNFKLSDASKMDFLDNFFDLV
                     FSVNVIHHIKDLPGYFKESYRVLKDGARILTATDSEWILRNRRPLTQYFPETLDVDLK
                     RYHPIEKIKKVMQDTGFVNIREEMTEYYYYLEDAGAYREKSFSSLNLISEDAF"
     gene            706..1533
                     /locus_tag="P5310_02940"
     CDS             706..1533
                     /locus_tag="P5310_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013683487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HRU32351.1"
                     /translation="MYRIVEKKRLSANVVQMVLEAPLIARSIKPGQFIIIMVYERGER
                     IPLTVVESLPQAGLITIVFQEVGKTTRLLGKKEVGEELFAIMGPNGNPFPAKLYGDVV
                     LVAGGVGVAEVVPIARALKDTGNNITTIVGARTKDLLFFTDVLGSISNNLYITTDDGS
                     SGIKGLVTLPLKELLDTVHIDMVLAVGPTIMMRSVANVTKSYGVKTYVSLNPIMVDGT
                     GMCGGCRVTVGGETKFVCVDGPEFDAHQVDFDELMQRQKTYIEEEKLALKLLEEGNG"
     gene            1526..2905
                     /gene="gltA"
                     /locus_tag="P5310_02945"
     CDS             1526..2905
                     /gene="gltA"
                     /locus_tag="P5310_02945"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HRU32352.1"
                     /translation="MAKTSRVPMREQEPEERIYNFNEVALGYNEEEALLEARRCLQCT
                     KPTCVEGCPVGIDIPAFIALIKEKRYRDAYTKIKEKCSIPAITGRVCPQENQCERSCV
                     LSKVREPIAIGRLERFVADYSAEEAIDIETKSSRDKRVAVIGSGPASLTVSSDLSRTG
                     YYVALFEALHLPGGVLTYGIPDFRLPKDIIKREFEYVIQLGVKFIPNVVIGKTILLDE
                     LLKDYDAIFIGSGAGAPMFLNIPGENLLGVYSANEFLSRINLMRAHHFPEYDTPIKIG
                     KKVIVIGGGNVAMDAARVSKRLGADVSILYRRTREELPARLEEVIRAEEEGINFMFLS
                     APLAFSSNNGWVNGIECQEMELGEPDASGRRAPIPKKGATFSLEADTVIIAIGNRPNP
                     IISRSTEGLKTGRNGEIIIDPATGKTSRVGVFAGGDIVSGSATVIEAMGAGRIAAMSI
                     HRYLTTGVW"
     gene            2978..3214
                     /locus_tag="P5310_02950"
     CDS             2978..3214
                     /locus_tag="P5310_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRU32353.1"
                     /translation="MPFYVYRCEGCGRELEVLQGINDEPLSRCSECGGVLKRLIFPPT
                     VIFKGTGFYCTDYRKGNPPKEEKEDSGGKNSDSG"
     gene            3183..3878
                     /locus_tag="P5310_02955"
     CDS             3183..3878
                     /locus_tag="P5310_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003600134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRU32354.1"
                     /translation="MVAEKILIVDDEKPIVDSIKYTLYKEGYDVVVSYDGEDALEKVR
                     KENPDLIILDIMLPKLSGLEVCRIIRRTSNVPIIMLTARGEDMDRVVGLELGADDYVS
                     KPFSMRELVARIKAVLRRAKMSVSTEAKTKEKLEFDDVLIDVKGRIVLKRGIPVDLSP
                     KEFDLLVTLAENEGRVMSREYLLNHIWGDDFYGDDRTVDVHIRWLREKLEDDPSNPEH
                     IQTVRGIGYILRT"
     gene            3875..>4507
                     /locus_tag="P5310_02960"
     CDS             3875..>4507
                     /locus_tag="P5310_02960"
                     /inference="COORDINATES: protein motif:HMM:NF013179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HRU32355.1"
                     /translation="MRRLALERLALNFILPALVLYLAFYLIVLSSIPYKYLFGAFILG
                     FTLISYYTLFRRIKLLLDRILMNIKELPSSGSVSPIGIPELDDSIREINEYINRFKEE
                     NKLYTRLFQSIQEGVIVVNKNREVLRINPTGLKLLNIENDKNLEGRPLIEAIWNYTLD
                     EMTERSIATGDVLSKEVTFDYLDSRIMDTLVSPMFNEKGEIRGAIIVFTDV"
BASE COUNT         1373 a          720 c         1099 g         1315 t
ORIGIN      
        1 aaaggcatct tcgcttataa gatttagaga ggaaaaactc ttttccctat aagcacctgc
       61 atcttccaga tagtagtaat actcggtcat ctcctctctg atgttgacaa acccggtatc
      121 ctgcatcact ttttttatct tttctatcgg atgataccgc tttaaatcca catccagtgt
      181 ctctggaaaa tattgggtga gaggtcttcg gttacggagt atccactctg aatcggtagc
      241 tgttaatatc ctggcaccat ctttcaacac cctgtaagac tctttaaagt atccagggag
      301 gtcttttata tggtgtatta catttacaga gaagacaaga tcaaaaaaat tatccagaaa
      361 atccatctta gaggcatctg acagtttgaa gtttacactc tttgatctat cctgggcttt
      421 agaaagcatc tctcttgaga tatctatacc ccaggactga cagcctgata taaagtttat
      481 cataattata tagtttccag ttccacaccc tatctccagg accttgctat ctttacttat
      541 attaataagc agactccgta atactcctgg gtgagtctcc ctatatctgg catactgcct
      601 ggctatttta tcaaagctaa aagacttatt gttacccatc ctctttctct tctaccggtt
      661 tttgtgatat tatatcatat taatttggga ataaggagag atgaaatgta ccgtatagtt
      721 gagaagaaga gactttcggc taatgttgta cagatggttc tggaggcgcc attaatcgcc
      781 aggtctataa aaccgggaca gtttataata attatggttt atgagagggg agagaggatt
      841 cctcttacag tggtggaatc tcttcctcag gctgggctta tcactatagt atttcaggag
      901 gttggaaaga ccaccaggct actgggtaaa aaagaagtag gcgaagagct cttcgctata
      961 atgggaccaa atggcaatcc atttccagct aaattgtatg gggatgtggt tctggtagca
     1021 ggaggtgtgg gggttgctga ggtggttccc atagccaggg cattaaaaga taccgggaat
     1081 aatataacca caatagttgg agccaggact aaggatttac tcttctttac tgatgtcctg
     1141 ggttctataa gtaataacct ttacattact acagatgatg ggtcaagtgg aataaaaggt
     1201 ttggttaccc tgcctcttaa agaactcctg gatactgtcc atatagatat ggttttggct
     1261 gttggtccta ccataatgat gaggtcagtt gctaatgtga ctaaaagtta tggagtaaag
     1321 acttatgtga gcttaaatcc aattatggtt gatggtacag gtatgtgtgg tggatgcagg
     1381 gttacagtgg gtggagagac aaaatttgta tgtgtcgatg gaccagagtt tgatgctcat
     1441 caggttgact ttgatgagct tatgcagagg cagaagacat atatagaaga ggagaaactt
     1501 gcacttaagt tactggagga aggaaatggc taagacttct agagtcccta tgagagaaca
     1561 ggaacctgaa gaacgtatct acaattttaa tgaggtcgct ctgggttata atgaagagga
     1621 ggcgttatta gaagccagaa gatgtcttca atgtacgaaa cccacgtgtg tagaaggatg
     1681 cccagttggg atagacatac cagcatttat agcattaata aaggagaaga ggtacaggga
     1741 tgcttataca aagataaaag agaaatgcag tatccctgcc attacaggaa gagtttgtcc
     1801 tcaggagaat caatgtgaaa ggtcatgtgt tctctccaag gttagagagc caatagctat
     1861 cggtagattg gagagatttg tggcagacta ttctgcagag gaagctattg atatagaaac
     1921 taaatcttct agagataaga gggtagcagt aataggctca ggtcctgcca gtcttaccgt
     1981 aagttcagat ctgtccagga caggatatta tgttgctcta tttgaggcat tgcatttacc
     2041 tgggggggta ttgacatatg gaatccctga ctttagactc cccaaggata taataaagag
     2101 ggaatttgaa tatgtgattc agcttggagt aaaatttata cccaatgtgg ttatagggaa
     2161 gacgatactc ttggatgaat tattaaaaga ttatgatgcg atatttattg gttctggtgc
     2221 cggagcccct atgtttttga atatacctgg agagaatctg cttggggtat actcagccaa
     2281 tgagttttta tcaagaataa atcttatgag agcccaccat tttcccgagt atgatactcc
     2341 aataaagatt gggaagaaag tcatagtgat aggtggaggg aatgttgcta tggatgcagc
     2401 aagggtttca aagagacttg gtgcagatgt tagcatcctt tacagaagaa ccagagaaga
     2461 attacctgcc aggttggagg aagtaatcag ggctgaggag gaagggatca acttcatgtt
     2521 tctctccgct cccttggctt ttagcagtaa taacggctgg gtaaatggga ttgagtgcca
     2581 ggagatggaa cttggagaac ctgatgccag tggcagaaga gcccctatcc ctaaaaaagg
     2641 cgctacattt tctttagaag ctgatactgt tataattgct ataggtaaca gaccaaatcc
     2701 cattatttct agatctactg agggcttgaa gaccggaaga aatggagaga ttattataga
     2761 ccctgccaca ggaaagacat cccgggttgg agtatttgct ggtggtgata tagtgagcgg
     2821 ttctgccacg gttatagaag ctatgggagc aggaagaata gccgctatgt ctatccatag
     2881 atatttaact acaggggtat ggtagtggta ttcttgtata agactattaa taagtgataa
     2941 aatatccttg agattcagat gggtggaggt gataaaaatg ccattctatg tatacagatg
     3001 tgaaggttgt ggtagagaat tggaggtttt gcagggtata aatgatgagc ccctatcgcg
     3061 gtgcagtgaa tgtggtggag ttctaaagag acttatcttc ccacccacag taatatttaa
     3121 aggaactgga ttctactgta cagattacag gaagggtaat cctcctaagg aggagaaaga
     3181 agatagtggc ggaaaaaatt ctgatagtgg atgatgagaa acctatagta gattctatta
     3241 agtataccct ctataaagag ggttatgatg tggtggtttc ttatgatggg gaggatgcct
     3301 tagaaaaggt aaggaaggaa aatcctgacc ttataatcct ggatataatg cttcccaaac
     3361 tgagtggact ggaggtctgc agaatcataa ggcggacctc caatgttccc ataataatgc
     3421 tcactgccag gggagaagat atggataggg tagtgggttt agaactgggt gctgacgatt
     3481 atgtctccaa gccattcagt atgagagagc ttgttgcaag gataaaggcg gtgctcaggc
     3541 gtgccaagat gtctgtatcc acagaagcta aaaccaagga gaaattagag tttgatgatg
     3601 tgcttataga tgtaaagggg agaattgtgc ttaaaagggg gattccagtg gacttatctc
     3661 ctaaagaatt tgatcttctg gtgactttgg ctgaaaatga ggggagggta atgagcagag
     3721 aatacctgtt gaaccatatc tggggtgatg atttctatgg ggatgataga acggtagatg
     3781 tccatataag gtggcttaga gagaagttag aagatgaccc tagtaatccg gagcatatcc
     3841 agaccgtaag ggggataggg tatattttaa ggacttgaga aggttagctt tagaaagatt
     3901 agctttaaac tttatcttac cagccctggt tctctatctg gctttttatt tgatagtact
     3961 ttcttctatc ccttataagt atctttttgg tgcatttatt ttgggattca ctcttatatc
     4021 ttactatact ctctttagaa gaattaaatt acttttagat agaattttaa tgaacataaa
     4081 agagttacca tcttctggca gtgtttcccc catcgggata ccggaattgg atgacagtat
     4141 acgggagata aatgagtata taaatagatt taaagaggag aataaacttt atacaaggct
     4201 atttcaaagc atacaggagg gggtaatagt agtaaataag aatagagagg tacttagaat
     4261 caatccaact ggactgaaat tattgaatat agaaaatgac aaaaatcttg aaggtcgacc
     4321 tttaatagaa gctatatgga attatacact ggatgaaatg acagaaagaa gtatagctac
     4381 tggtgatgtt ttgtctaaag aggttacctt tgattactta gatagtcgta tcatggatac
     4441 cctggtctct cctatgttta atgagaaggg agagattaga ggggctataa tagtttttac
     4501 cgatgtt
//
LOCUS       DAOTKU010000083         4454 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_16851_length_4454_cov_3.049557, whole genome shotgun sequence.
ACCESSION   DAOTKU010000083 DAOTKU010000000
VERSION     DAOTKU010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4454
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16851_length_4454_cov_3.049557"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="P5310_02965"
     CDS             <1..381
                     /locus_tag="P5310_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32356.1"
                     /translation="MLQYTEREMRWKILRATPQIWKNRGIDILVTHSPPLMCVGESQG
                     RCKSPVGIGKRCPENEQGICEESKDLAHRGFKVLREFDLKYKPKVHLHGHTHLDYLNR
                     RKNRIKTLGDTKVIDCYGYYILEL"
     gene            complement(378..1181)
                     /locus_tag="P5310_02970"
     CDS             complement(378..1181)
                     /locus_tag="P5310_02970"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRU32357.1"
                     /translation="MLIAELYKLFKQRQVYYTFLGSLLLVVALIIVAKSGANLEIQIQ
                     TGSNIDLRPLINAYFVMGFTLTAGMPFLLPLFVSLNTSNIFTREFSEGTMRTYLVRVG
                     GRRRIFINKVMANFIITVLLVAFTGVISFSLGAIFFGTEAKTFISPLLPIPIPFSWED
                     NFLPAYLCSSVCLMTIASLSVLWSVTSGATGETLLKTLSTIIILSLFSNIQRVRPYLF
                     TSYLDIWNKAWSDPIPWAELNRGSLILLLYSLGFLVIAYIIFSRKDVLS"
     gene            complement(1150..2091)
                     /locus_tag="P5310_02975"
     CDS             complement(1150..2091)
                     /locus_tag="P5310_02975"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRU32358.1"
                     /translation="MYGAGEPVIQTFGLTKSFKNKLAVDGLSLTVHRGDIYAFLGQNG
                     AGKSTTIKMLLGLLYPSKGYAEILNTKVPGELCKVLPRIGVVTESPAFYPYLTGEENL
                     SLYGGLLGIKDKKKIKDTLERVGLLGVKQKVSEYSQGMRQRLALGLATLNGPELLMLD
                     EPTNGLDPQGIYEIKSIIRDMNKNGVTIFLSSHILSEVQEIASRVGVIHKGKLIAEGL
                     VSELLAFGDRITVMASPRDLVLKVLKENPIVKDFTEDTDKFIIYIDKENVSTLNREMV
                     NAGIDVFALIPVRSTLEDFFISITGDDNVNSGTLQAI"
     gene            complement(2075..2329)
                     /locus_tag="P5310_02980"
     CDS             complement(2075..2329)
                     /locus_tag="P5310_02980"
                     /inference="COORDINATES: protein motif:HMM:NF013999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HRU32359.1"
                     /translation="MVDKEKVITALRDVYDPEIGVNVVDLGLIKELKIDNDNVTVDMV
                     LTIPGCPLREYMISQVRETVESVEGVKSVTVNLLDDVWSW"
     gene            2427..3221
                     /locus_tag="P5310_02985"
     CDS             2427..3221
                     /locus_tag="P5310_02985"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HRU32360.1"
                     /translation="MRIKLIAIDIDGTLITPDKIVTPYVREALRYAEDRGIMVSLVTG
                     RLYPTCRKYSLELSLSGPCVIYQGAMIIDPKTDKVLYEVRIPRDKALKIVKIAREKNL
                     SLNVYMDQFTFYTERPNQYSILDAQLNEVDIQIVKDIEKIIEYDPLKLMFVDEPGVIS
                     RLEENFSSYDGDLVAFTSLPQFLEIVNRSATKADALKWIANRFNIKREEVVAIGDSHN
                     DIPMLEWAGIGVAMGNADDKVKGAADFITLSNREDGVAHAIEKLIK"
     gene            3303..>4454
                     /locus_tag="P5310_02990"
     CDS             3303..>4454
                     /locus_tag="P5310_02990"
                     /inference="COORDINATES: protein motif:HMM:NF024900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HRU32361.1"
                     /translation="MALVNIKVNGQPVQVEEGKTVLEALKEAGIDVPVLCYHPELPSI
                     GACRMCMVETRPGVLQASCTLPVSEGLELQTDSETVTKARKFVLNLIFSERNHYCMYC
                     EMSNNCELQDLGYKLQLDHFDIPTYTTRFPVDATHNYILLDHNRCILCRRCVRACSEI
                     AGHNVLGISQRGIESLLTADLNLPFGESSCVSCGLCVQVCPTGALTDKNTAYLGTRRE
                     SEMIKSICANCSVGCGIEAYKRANNLVQILGDWDSPVNNGILCALGRYTPVAEHRERI
                     LQPMVKKNGKLETATWEEALEKVREILNTGEVTGYISGDLPLEVLSGFKDLMKGNAGL
                     LENTSNPIDNSGKLEDILVADKILVIGVDLDKSFGVVGSYVKKRVFNGGE"
BASE COUNT         1467 a          836 c          894 g         1257 t
ORIGIN      
        1 atgcttcagt atacggagag agagatgagg tggaaaatat taagggctac ccctcagata
       61 tggaagaata gaggaataga tatacttgta actcattcac cacctcttat gtgtgtaggt
      121 gaatctcagg gaagatgtaa atctccggta ggtattggga agagatgtcc agaaaatgaa
      181 cagggaatct gtgaagaatc aaaagacctt gctcacaggg ggtttaaagt actcagagag
      241 tttgacctga aatataaacc caaagtccat ctccatggac atacacatct tgattaccta
      301 aacaggagaa agaatcgtat aaaaacactg ggggatacta aggtcataga ctgctacggt
      361 tattatattt tagagctcta agataaaaca tctttcctgg agaatattat atatgctatt
      421 acaagaaaac ctagtgagta aagaagtaag atcagggaac ctctgtttaa ctctgcccag
      481 gggattggat cactccatgc cttattccat atatccagat aagacgtaaa aaggtagggt
      541 cttaccctct ggatgttaga aaacaaactg aggataatta tggtggataa tgtcttaaga
      601 agagtttcac ctgtagcccc tgaagtaaca gaccagagta ctgagagact tgctattgtc
      661 attaaacata cactgctgca taaataggca gggagaaaat tatcttccca gctaaatggt
      721 atcggtattg gaagtaatgg tgagataaat gttttagctt cagtcccaaa aaatatagca
      781 cccagagaaa agcttatcac cccggtaaac gcaacaagaa gtacagtaat aataaaatta
      841 gccataacct tatttataaa tatccttctt cttccaccga ctcgtacaag ataagtcctc
      901 attgttccct cggaaaattc tcttgtaaag atatttgagg tatttaaaga tacaaacagt
      961 ggtagaagga atggcatccc tgcagtaaga gtaaagccca taacaaaata ggcatttatt
     1021 aatggacgta aatcaatatt agaaccagtc tggatctgaa tttccagatt tgccccagat
     1081 ttagctacta tgatgagagc aaccaccagt aggagagaac ctaaaaatgt ataatagacc
     1141 tgtctctgtt taaatagctt gtaaagttcc gctattaaca ttatcatcac ctgttatact
     1201 gataaagaaa tcctcaaggg tggacctgac agggatgaga gcaaatacat ctatccctgc
     1261 attaaccatt tctctattaa gtgtagaaac attttcctta tctatataga taataaattt
     1321 atcggtatct tcagtaaaat ccttaacaat aggattttct ttaagcactt ttaaaaccaa
     1381 atctcttgga ctagccataa ctgttatacg gtctccaaat gccaaaagtt cacttaccag
     1441 cccctcagct ataagcttac ctttatgaat aacccctacc ctgctggcta tctcctgaac
     1501 ctcagaaagt atatgactgg ataaaaatat cgtcacccca tttttattca tatcccttat
     1561 tatgctcttt atctcatata tcccttgagg gtccagccca ttggtgggtt catccagcat
     1621 caaaagctct ggaccattca gggtagcaag accaagagct aacctctgtc tcatcccctg
     1681 agaatactct gaaaccttct gctttacccc tagcaaacca actctttcta aagtatcctt
     1741 tattttcttc ttatccttta ttccgagaag acctccatag agagatagat tttcttcacc
     1801 ggtaagatac ggataaaaag caggagactc agttaccacc ccaattctgg gtaatacttt
     1861 acataactct cctggcacct tggtatttaa gatctcagca taacccttag atggatagag
     1921 aagacccagt aacatcttta tggttgtact tttccctgca ccattctgcc ccaggaaggc
     1981 atatatatcc cctcgatgaa cagtgaggga tagcccatca accgccagct tgttcttgaa
     2041 gctctttgta agaccaaatg tctgaattac aggttcacca gctccataca tcgtctaaca
     2101 ggtttacagt aactgattta accccctcta cagattctac agtctctctt acctgagaga
     2161 tcatatattc tctaagtgga cagcctggaa tagtaaggac catatccaca gtaacattat
     2221 cgttatctat ctttaattcc tttatcagcc cgagatcaac cacatttact ccaatctccg
     2281 gatcataaac atccctgaga gcggtaataa ctttctcttt atctaccatt atgataaatt
     2341 tcctcctaag ttaaaactct ttatcagtat agtattaacc gtatcccctt gtcaatagta
     2401 tcttatataa tgtaaaatca ttatctatga gaataaaact tatagctata gatatagacg
     2461 gaacattaat aacccctgac aaaatagtaa ctccatatgt aagagaggca ttgaggtatg
     2521 cagaggatag aggaattatg gtctctttgg ttacaggaag attatatcca acctgcagga
     2581 aatactctct ggagttaagc ctttctggtc cctgtgttat atatcaggga gctatgatta
     2641 tagatccaaa aacagataaa gttctatatg aagtaaggat tcccagagat aaagctctca
     2701 agattgtaaa aatagcaaga gaaaagaacc tgtctctaaa tgtatatatg gaccaattta
     2761 cattttatac cgaaagacct aatcagtatt ccatactgga tgcacaactg aatgaggttg
     2821 atatacagat agttaaagac atagaaaaaa taatagaata tgaccctctt aagcttatgt
     2881 ttgtagatga accaggtgta atatccagat tagaagaaaa tttttcatct tatgatggag
     2941 acctcgttgc cttcacatcg cttccacaat ttctggagat agtcaataga agtgccacca
     3001 aggcagacgc attaaaatgg atagcaaaca ggtttaatat caaaagggag gaggtagtag
     3061 ctatagggga tagtcataat gatataccaa tgttagagtg ggcaggtata ggggtagcta
     3121 tgggtaacgc cgatgacaag gtaaaagggg cagctgattt tatcacactg tcaaatagag
     3181 aggatggagt tgcccatgcg atagaaaagc taataaaata gaggaataat ttagcttgac
     3241 ttaatcgtga tttatgtata aaatatctta ggtaatttct tagattgaat aggaggtctt
     3301 agatggcttt ggtaaatatc aaagtaaatg gacagccagt gcaagtagaa gagggtaaga
     3361 cagttcttga agctttaaaa gaggctggta tagacgttcc tgttctctgc tatcatccag
     3421 aactaccatc aataggtgcc tgcaggatgt gtatggtaga gacaagaccc ggagtgctgc
     3481 aagcttcctg tacactaccg gtatctgaag ggctggagct tcaaacagac tctgaaacgg
     3541 taacaaaagc gagaaaattt gttctaaatt taatcttttc agagagaaat cactactgta
     3601 tgtactgtga gatgagcaat aactgtgaac ttcaagattt gggatataag ttacagttag
     3661 accattttga cattccaaca tatactacca gattccctgt ggatgctaca cataattata
     3721 tactcttaga ccataacagg tgtattttgt gcaggagatg tgtcagagcc tgctcagaga
     3781 tagcgggaca taatgtgctt ggtataagtc agagaggaat agaatccctg cttacagcag
     3841 atttgaattt accttttgga gaatcttcct gtgtttcatg cggtctctgc gtacaggtat
     3901 gcccaaccgg tgccttaact gataagaata ccgcctacct tggtaccaga agagagagtg
     3961 aaatgataaa gagtatctgc gcaaattgca gcgttggttg tgggatagaa gcttataaga
     4021 gagccaataa tctggttcaa atcctgggtg actgggatag tccggtaaat aacggaattc
     4081 tctgtgcact tggtagatat acacctgtgg ctgagcatag agagagaatc cttcagccta
     4141 tggtgaagaa gaatggtaaa ctggaaactg caacctggga agaagctctt gagaaagtaa
     4201 gagagatatt gaataccgga gaagttacag gttacatctc cggggatctt cctcttgagg
     4261 tactctctgg cttcaaagat ctgatgaagg gaaatgctgg acttttggag aataccagta
     4321 atcctataga taattcgggt aaattagagg acatactggt tgcagataag atactagtta
     4381 taggggttga tctggataaa agttttggag ttgtaggctc ctatgtaaaa aagagagtct
     4441 ttaatggtgg agaa
//
LOCUS       DAOTKU010000084         4404 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_17052_length_4404_cov_3.177282, whole genome shotgun sequence.
ACCESSION   DAOTKU010000084 DAOTKU010000000
VERSION     DAOTKU010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4404
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17052_length_4404_cov_3.177282"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1062)
                     /locus_tag="P5310_02995"
     CDS             complement(<1..1062)
                     /locus_tag="P5310_02995"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRU32362.1"
                     /translation="MRKTLSIFLSLMLAFALILGVITVSFSATQKIDPGGQYNLSDYQ
                     RLTGKKITKFNEAPQLRELVKQGKLPPVEERLSSSPAIVQPIEKVGEYGGTWRRYTPL
                     AAYGINFLISYEPLVRICGPDLTKIEPNIAERWNLSKDTKTLTLYLRKGIRWSDGESF
                     TADDIIFWYEDILLNKELTPTIPTWLIVGGEVAKIKKIDNYTIEMNFSQPYPLILYEL
                     AAAKYPYAPKHYLKQFHPKYTPKEKLDAMVKEAGLQFWYQLFAQKNDATVNTELPVIW
                     AWRVTIPWGPDGTKAERNPYYWKIDPEGNQLPYIDTIRAYYVSDWNIGFLKIMAGEGE
                     MQGVGFRLEDYDLYMSNREK"
     gene            complement(1225..2394)
                     /locus_tag="P5310_03000"
     CDS             complement(1225..2394)
                     /locus_tag="P5310_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HRU32363.1"
                     /translation="MRRESTIILVLNTIRKYGPINRYEIAKKTSLSPATITDVTDKLI
                     KMGFISEVPSSGKKRGRRPINLSLSNSTHIAVGIEIDSRYISGVAIGLGGDILKRKKI
                     DIETSDPASIFSETVRLYRSLTEDLEKERFLGLGLGIPGLIDPKNKRVIFSAVLGWKD
                     VYVEEFLNGLGIPFFMEDNVRAITLGELWYGAGIGKDNLLCIGAGRGISAGIVINGSI
                     YSGPNFRAGEFGHMVIEKDGRKCKCGNYGCLEPYVSTETIIEKVLEGIENNAYTRFSP
                     INEDRLFEEIIQAGKEGDKFILNIFEEVGEYLGIGIANIINFFNPELIILADELAEAG
                     ELILEPVKRAIRLHALPPIPEITVSKLGSLSCPIGSATLVIKELFRKDLDRYINL"
     gene            2571..3122
                     /locus_tag="P5310_03005"
     CDS             2571..3122
                     /locus_tag="P5310_03005"
                     /inference="COORDINATES: protein motif:HMM:NF015935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HRU32364.1"
                     /translation="MDKEGTLDYLAIKNIYPMYSRKGKVFLNGKDAKSLTQKEREEFI
                     GILQIKSLEDCKKIREEIKGKANNHRVNNFAKAIQIKAALELGKRLNREEVEFGLKIK
                     NPKDVVRYVKEYYSPYIKDTNKEIFNLTLLDYRNKVIGNIELSKGNVNSGIVDPRKII
                     REATVKSASFVVLVHNHPLGLIK"
     gene            3136..4362
                     /locus_tag="P5310_03010"
     CDS             3136..4362
                     /locus_tag="P5310_03010"
                     /inference="COORDINATES: protein motif:HMM:NF025684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HRU32365.1"
                     /translation="MKRRLLVLLILGLFIIPTLIQGAEPELKTIIIEPPPGAEFKVDV
                     WTDKGDNAKYNVGEKLIIYFKVTQDAYVYIWDINANGEVRLIFPNKYNQDNFVRANIV
                     YSIPSSRDTYSLKIAPPAGREVIHILASKTPISTLEEYKGMMDRDPFPLIPQAPEIFT
                     DKVKRTIEIVPTPTQWTTDNAIFYVAEGPTSTTTGRLIVESNPRGASITLDGRSLGYT
                     PYDGQVPVGRHTVRLDLAGYQPYTTEVNVTTTQISRIDVRLTPIPAATGRLIVESNPR
                     GASITLDGRSLGHTPYDGQVPVGRHTVRLDLAGYQPYTTEVNVTPNKTAKIEVKLNPI
                     LSMEGEFNITSIPSNAEVFINGERKGKTTLRVKKLKPGRYQVTIIKPGYETFVAYYEV
                     FPGQTTNINISLNPIR"
BASE COUNT         1393 a          866 c          788 g         1357 t
ORIGIN      
        1 tttttctcgg ttcgacatgt aaaggtcata atcttccaat ctaaaaccta ccccctgcat
       61 ctccccttca ccagccatta tctttaaaaa acctatgttc caatctgaaa cataataagc
      121 cctgatagta tctatataag ggagttgatt cccctctgga tctatcttcc aataataagg
      181 gttcctttca gccttcgtcc catctggacc ccaaggtata gttacacgcc aagcccaaat
      241 gactggaagc tcagtattaa cagttgcatc attcttttgg gcaaagagtt gataccagaa
      301 ttgtagcccc gcctctttta ccattgcatc aagtttttct tttggggtat atttaggatg
      361 gaattgtttt agatagtgtt taggggcata agggtattta gctgcagcta actcataaag
      421 gattagagga tacggttgag aaaaattcat ctcaatagta tagttatcta tttttttaat
      481 cttagctact tctcccccta ctattaacca tgtaggaata gttggagtaa gctctttgtt
      541 aaggagaata tcctcatacc aaaatataat atcatcggca gtaaaggatt ctccatctga
      601 ccatcttatc cctttcctta aatacagagt tagtgttttt gtatcctttg aaagattcca
      661 tctttctgcg atgtttggtt ctatcttagt tagatctgga ccacagatac gtactagtgg
      721 ctcataggat attagaaaat taatgccgta cgccgccaat ggagtatatc ttctccaggt
      781 tccaccatat tctcctacct tctctattgg ttgaactatt gcaggactac tagataacct
      841 ttcttcgaca ggaggaagtt ttccctgttt taccagctct cttaattgag gcgcctcgtt
      901 aaatttggtt atctttttac ctgtgagtct ctgatagtca gagaggttat actgaccccc
      961 aggatctatc ttctgtgtag cagaaaaact tacagttata actcccaaga tcaaagcaaa
     1021 agctaacatc aaactgagaa atatcgataa cgtttttctc attactttat tcctcctttt
     1081 aaatttatta atcttaagcc ctcctctttt gcctcatcag actattccgg tcttttatta
     1141 gtttaaaata agtttctcct tgatcaccac ctcctctctt tgtttgctcg aaattctctc
     1201 ctgttcattc ctcaatggta cctatcatag attgatatac ctatccaggt ctttgcggaa
     1261 cagctctttt attaccagtg tggcagaccc aataggacag gacagactgc ctagttttga
     1321 aacagttatc tcagggatag gaggaagtgc atggagtctt atcgctcttt ttactggctc
     1381 aagtataagt tctcctgcct cagctaattc atcagccaga ataattagtt cagggttaaa
     1441 aaagtttata atattagcga tgcctatacc caggtactct ccaacctctt caaatatgtt
     1501 caggataaat ttatctccct ctttaccagc ctggattatc tcttcaaata atctgtcttc
     1561 attaatgggt gaaaatcttg tatacgcatt attctcgata ccctccagta ccttctctat
     1621 aattgtttct gtggagacat aaggttcaag gcatccgtaa tttccgcatt tacactttct
     1681 accatctttc tctatcacca tatgtccaaa ttctccagca cggaaatttg gtcctgaata
     1741 tatagaacca ttaataacaa tacctgcact tatacctctc ccagcaccta tacagagaag
     1801 attatctttc cctatccctg ccccgtacca gagttccccc aaagtaatag ctcttacgtt
     1861 gtcttccata aaaaagggaa ttcctagacc gttgaggaat tcttccacat atacatcttt
     1921 ccagcctaaa acagcggaaa agattactct tttattcttt ggatctataa gaccagggat
     1981 tccaagaccc aaacctaaaa atctctcttt ttctaaatcc tctgtaagtg atctgtaaag
     2041 tctaactgtt tcactgaata tagatgctgg atcagatgtt tctatgtcaa ttttttttct
     2101 ttttaaaata tctccaccca atcctatagc taccccactt atatatctag aatctatctc
     2161 tatccctaca gcaatatgag tactattact caaagaaaga tttataggtc ttcttcctct
     2221 ttttttacca gatgagggta cttcagaaat aaagcccatt ttgataagtt tgtcagtaac
     2281 atctgtaata gtggcaggac ttaaagatgt cttttttgct atctcataac gatttatagg
     2341 accgtatttt cttatcgtat ttaataccaa tattattgtt gactctcttc tcaaaagaat
     2401 actcctattt tttattttct aaaattataa cactttaaaa tcccctgtca atagatttca
     2461 aggtttagaa tataaaatta aaaggactgt ttcctctgtt catttttcga actttaaaat
     2521 gaaaaagcac atattatctc tgataaagaa ttatgttata atgattgaag atggataaag
     2581 aagggacact tgactatctg gcaattaaga atatatatcc gatgtatagc aggaagggga
     2641 aggtcttctt gaatggaaaa gatgctaaga gtttaactca gaaggaaaga gaagaattca
     2701 tagggatatt acagataaaa agtctggaag attgtaaaaa gataagagaa gagataaaag
     2761 gaaaggcgaa taatcatagg gtaaataact ttgctaaggc aatacagata aaagcagcat
     2821 tagagcttgg caaaagatta aatagagagg aggtggaatt tggattaaaa ataaagaatc
     2881 caaaagatgt ggtcagatat gtaaaggaat attattctcc gtacatcaag gatactaata
     2941 aagaaatatt taatctgact cttttagact atcgaaataa agtgattggt aacattgagt
     3001 tgagcaaggg taatgtaaat tctggcatag tggatcccag aaaaataatt agagaggcta
     3061 cagtaaagtc tgcatctttt gtggttctgg tacataatca tcctttggga ctaataaaat
     3121 aggaggtaga ggaagatgaa aagaaggtta ttagtactgc ttatcttagg attatttatc
     3181 atacccactc taattcaggg agctgaacct gagttaaaaa ctattattat agaaccaccc
     3241 ccgggtgcag aatttaaagt agatgtctgg actgataagg gagataacgc taaatataat
     3301 gttggggaaa aattaattat ctatttcaag gtaactcagg acgcatatgt gtatatatgg
     3361 gatatcaatg ctaatggaga agttagactt atatttccta ataaatacaa tcaggataac
     3421 tttgttagag ctaacatagt gtattctatc ccttcttcga gggatactta tagcctaaag
     3481 attgcacctc ctgctggaag agaggtaatt catatcctgg ccagtaagac acctatctcc
     3541 actctggagg agtacaaggg gatgatggat agagatcctt ttcctctcat cccgcaagcc
     3601 ccagaaattt ttaccgataa ggtaaaaaga accatagaga ttgtcccaac ccccacacaa
     3661 tggacaacag ataatgctat cttctacgtt gcagaaggtc caacatctac taccactgga
     3721 agactgatag tggagagtaa ccccaggggt gctagtatta ctctggacgg aagatcctta
     3781 ggttatactc catatgatgg acaggtccct gtaggaagac acactgtaag actggacctt
     3841 gccggctatc agccgtatac tacggaggta aatgtaacaa ccacacaaat ttccaggatt
     3901 gacgtcagac ttacacccat acctgccgct actggaagac tgatagtgga gagtaacccc
     3961 aggggtgcta gtattactct ggacggaaga tccttagggc atactccata tgatggacag
     4021 gtccctgtag gaagacacac tgtaagattg gaccttgccg gctatcagcc gtatactacg
     4081 gaggtaaatg taacacccaa taagacagct aaaattgaag taaaacttaa ccctatatta
     4141 tctatggaag gggaatttaa tataaccagc atcccttcta atgctgaggt atttataaat
     4201 ggagaaagaa agggaaaaac aaccctgaga gtcaaaaagc taaagccagg aagatatcag
     4261 gtaacgataa ttaaacctgg atatgagact ttcgtggctt actatgaggt ttttccagga
     4321 cagacaacca atattaacat atcactaaat cctataagat agtcagaata actaggtgag
     4381 taatcgcaag gtgtgatgat gcca
//
LOCUS       DAOTKU010000085         4377 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_17161_length_4377_cov_3.095558, whole genome shotgun sequence.
ACCESSION   DAOTKU010000085 DAOTKU010000000
VERSION     DAOTKU010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4377
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17161_length_4377_cov_3.095558"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="P5310_03015"
     CDS             <1..194
                     /locus_tag="P5310_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32366.1"
                     /translation="VHKILDPYGNLHFWIGGIPLNETDQDSEDFLLREGYIVISPLKV
                     DKTDYQFLEVIKTWKIGLD"
     gene            173..997
                     /gene="thiM"
                     /locus_tag="P5310_03020"
     CDS             173..997
                     /gene="thiM"
                     /locus_tag="P5310_03020"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: protein motif:HMM:NF006830.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HRU32367.1"
                     /translation="MEDRIGLILGEGKNLWDKLRSINPLVHHITNFVAMPEQAHATLA
                     IGASPVMALYPEESGDMCSIANSLLINIGIPSTELLSAMKKAMEVANKNDIPVLLDPV
                     GYGSTPFRNYVVDNLLKEYRVTVVKGNRGEITVLAGGSGMVRGVDSVNGVGTDLKDIV
                     KSLATRYRAIVVATGREDFVSNGTDVISVRGGSELLRDVTGSGCIAGSIISAVLGVRG
                     DPFISSITGLIAFKLSARRAEARSNKPGSFRMYLFDELSSINGEDIKREGEIVIWT"
     gene            988..1608
                     /gene="thiE"
                     /locus_tag="P5310_03025"
     CDS             988..1608
                     /gene="thiE"
                     /locus_tag="P5310_03025"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011012480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HRU32368.1"
                     /translation="MDLKLYVITDEKVGLSRSHSFLAEEALKGGATAIQLRDKEKGGR
                     ELYRIGVKIRELTRRYKALFIVNDRLDIAMAVGADGVHLGTEDLPVSVARRIAGKNFI
                     IGASVSSPEEALLAEKEGADYISAQSIFRTSSKENVKIIGLEGLRSIVNASSLPVIAI
                     GGINKDNVKDVIRNGAKGIAVISAAVSEEDVKKAVEELRESLEEVL"
     gene            1728..3047
                     /locus_tag="P5310_03030"
     CDS             1728..3047
                     /locus_tag="P5310_03030"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HRU32369.1"
                     /translation="MMAEKSEYCIAIIGTGFVGLTTGAGLSELGNKVICVDIDEGKIS
                     SLSNGYVPIYEEGLFELVERNRKLHRLFFTTDIEEATNSSDIIFICVGTPSKDDGDVD
                     LSQIISAVEGISNSLNSYKIIAIKSTIPVGTLELVEGILGKKDKIKGRDYDLAVVPEF
                     LREGKAVYDFFNPTRIVIGADKDDVSKVLAGIFSPLNAPVVYTTPLTAQLIKYASNAF
                     LASRISFINEIANICDHLGINVKDVIEGMKYDKRIGGHYLSPGIGFGGPCLSKDLNGL
                     IKMAESYGYQPNYLKSIMEKNSHQISYVIDKLDKLLGGFRPGTKIGILGLTFKPDTND
                     VRNSLSIRIIKEIRERGSGVRAYDPKGMEEAKKYIPDIIFCNDLYEAAKDSDALLILT
                     AWDEFKEIDLERIRSTMKNPIIVDGVNILDPAKVKRFGFLYEGIGVR"
     gene            3054..4208
                     /locus_tag="P5310_03035"
     CDS             3054..4208
                     /locus_tag="P5310_03035"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HRU32370.1"
                     /translation="MGSNVAVLSCYEYDKDNIKATIKEGFSHIGIPEIRNKSILIKPN
                     LLMPAEPHYGVTTHPVMIEAVGEILLEFGAREILIGDSPGNALSNIENLYKRTGISSL
                     ENKEGFRLVNFSREGIIDVDNPGGMVPVFPLTKIIKEVDYIVNMPKLKTHNFTLVTCA
                     IKNMFGSIPGFNKSKMHAVAPSPEGFSRLLVEIFRSVGPDLNIVDAIEGMEGDGPSGG
                     TPRRFGRILMGYDPVAIDAVGGWMLGYDPRDIYTTRIAGAMGLGENSLENIEIFGAKL
                     EDLFGHDVKKVNTVYNVTNKLSSPIFSFLSKRLVKFIKIYPEIDDDKCVRCEACLRSC
                     PQKAIDKFDGRMKINYKKCISCYCCHELCPQKAVMIKKNRLADILWLARD"
     gene            4237..>4377
                     /gene="nadA"
                     /locus_tag="P5310_03040"
     CDS             4237..>4377
                     /gene="nadA"
                     /locus_tag="P5310_03040"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010884133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HRU32371.1"
                     /translation="MDIVKKIRNLAKEKDAIILAHNYTRPEIQDIADFVGDSLELSRT
                     ASK"
BASE COUNT         1413 a          630 c         1073 g         1261 t
ORIGIN      
        1 gggtacataa gatacttgac ccctatggaa atctccactt ctggatagga ggtatacccc
       61 tgaatgaaac cgaccaggac agtgaggatt ttcttcttcg agaaggatat atagttattt
      121 caccattaaa ggttgataag actgactatc agtttttgga ggtgataaag acgtggaaga
      181 taggattgga ttgatattag gtgagggtaa aaatttgtgg gataaattga gatctataaa
      241 ccctcttgtt catcatatta ctaattttgt tgctatgcca gaacaggctc atgccacttt
      301 ggctattggt gcaagcccag ttatggctct ttacccagaa gaatccggcg atatgtgttc
      361 tattgctaat agtcttctta taaatatagg gattccttct acggagcttt tatctgctat
      421 gaaaaaggct atggaggtgg ctaacaaaaa tgatatccct gttcttctgg acccggtagg
      481 ttatggatca actcccttca gaaattatgt ggtagataat cttttaaaag aatacagagt
      541 aacggtggta aaggggaata ggggagagat aacagttctg gctggagggt ctggaatggt
      601 aaggggtgta gattctgtta atggtgtcgg tactgattta aaggacatcg tgaagtcttt
      661 agccaccagg tacagagcaa tagttgttgc caccgggaga gaagatttcg tctccaatgg
      721 gaccgatgtt atttcagtaa gaggtggaag cgaattactg agggatgtaa cagggtctgg
      781 ttgtattgct ggctctataa tctcggcggt actaggtgtc agaggtgatc catttatatc
      841 ttctatcaca ggattgatag cctttaaact gtcagctaga agggcagaag ccagaagtaa
      901 taaacctggc agtttcagga tgtatctctt tgatgaacta tcctctataa atggtgaaga
      961 tataaaaaga gagggagaaa tagtcatatg gacctgaaac tttatgtgat tacagatgaa
     1021 aaggttggtc tgagtaggag tcatagtttc ctggcagaag aggctttaaa gggaggagct
     1081 actgcgatac agctcagaga taaagaaaaa ggtggaagag agctgtacag gataggggtt
     1141 aagataagag aacttaccag acggtataaa gcacttttca tcgtaaacga caggttagat
     1201 atagctatgg cagtaggggc agacggagtt catcttggga ctgaagattt accggtatct
     1261 gtggcgagaa gaatagccgg caaaaatttt ataattggag cttcggtaag ttctcctgag
     1321 gaggcattgc tggctgaaaa ggagggggcg gattatataa gtgctcagag tattttcaga
     1381 acttctagta aagagaacgt aaagattata ggacttgaag gacttagatc tatagtaaat
     1441 gcctcatccc tgcctgtgat agctataggt ggtataaata aggataatgt aaaggatgta
     1501 ataaggaatg gggctaaggg tatagctgta atatcggcag cagtatctga agaggatgtg
     1561 aaaaaggcag tggaagaatt aagagaatca ctggaagaag ttttatagtg aattaataac
     1621 cagccctagt tgaagctttt tgtgagattt tgatacttta tatagatggt tgaaatatga
     1681 taagataact ataaactaat atgtttaaca acaggagatt tgaagatatg atggcggaga
     1741 aaagtgaata ctgtatagct attataggga ctggatttgt tggattgacc actggagctg
     1801 gtctgtcaga actgggtaat aaggtgatat gtgttgacat agatgaagga aagataagca
     1861 gtctcagtaa tggatatgtt cctatatacg aagaggggct atttgaactg gttgaaagaa
     1921 atagaaagct tcacagatta tttttcacca cggatataga ggaagcaaca aattcaagtg
     1981 atattatttt tatatgcgtt ggaaccccat ctaaagatga tggagatgta gatctctcac
     2041 agataatctc tgcagttgaa gggatcagta actctttaaa ttcctataaa attatagcga
     2101 taaaaagtac tattccggtt ggaacactgg aacttgtaga gggtatactg ggtaaaaagg
     2161 ataagataaa aggaagagat tacgatttag cggtagttcc agagttttta agagagggga
     2221 aggcagtata cgatttcttt aatccaaccc ggattgttat aggggctgat aaagatgatg
     2281 tgtccaaagt tctagcggga atattttcac ctctcaatgc acctgtagta tatactaccc
     2341 cactaactgc tcaacttatt aaatatgcct ctaacgcctt tcttgcctcc aggatatctt
     2401 ttataaatga gatagcgaat atctgtgacc atcttggtat taatgtaaaa gatgttatag
     2461 agggaatgaa gtatgataag agaattggag gacactatct ttctcctggt ataggttttg
     2521 gtggaccatg tttatctaaa gatttgaatg gtcttataaa gatggctgag agttatggat
     2581 atcaaccaaa ctatttaaag tctattatgg agaagaatag ccatcagatt tcctatgtga
     2641 tagataagct ggataaactc ttgggaggtt ttcgccctgg cacgaaaatt ggtatactgg
     2701 ggcttacatt caaacctgat actaacgatg tcaggaattc tttgtctata aggattataa
     2761 aagagataag ggagaggggc tccggagtaa gagcctatga ccccaaaggt atggaagagg
     2821 ctaaaaaata catccctgat atcattttct gtaacgattt atatgaggca gccaaggata
     2881 gtgatgcctt actcatatta accgcctggg atgagtttaa agagatagat ttggaaagga
     2941 taaggtctac catgaaaaat ccaataattg tggatggggt aaatattctg gatccagcaa
     3001 aagtcaagag atttggtttc ctttacgaag gaataggggt aagatagtat tatatggggt
     3061 ctaatgttgc agtattaagc tgttatgagt acgataagga taatataaaa gctacaatta
     3121 aagagggctt cagtcatata ggaatccctg agataagaaa taaatctatt ctcatcaaac
     3181 ctaatcttct tatgccagca gagccacatt atggggtgac tactcatcca gtgatgatag
     3241 aagcggtagg ggaaatacta ttggaatttg gagccaggga aatcctcata ggtgatagcc
     3301 ctggaaatgc tctttcaaat atagaaaatc tgtataagag gaccgggata tcttctctgg
     3361 agaataaaga gggttttaga cttgtaaact tttccagaga agggattatc gatgtggata
     3421 accctggtgg aatggtacca gtttttcctc ttaccaaaat aataaaagaa gtagattata
     3481 tagttaatat gccgaaactt aaaactcata actttacact ggtaacctgt gctataaaga
     3541 atatgtttgg ttcgatccct ggatttaata agtccaagat gcacgctgtg gctccaagtc
     3601 ctgaaggatt ttccagactt cttgttgaga tattccgttc ggtaggacct gatcttaata
     3661 tagtagacgc tattgaaggg atggagggag atggtccttc aggtggaaca cctagaagat
     3721 ttggtcgaat acttatgggt tacgatccag ttgcgataga tgctgtcgga ggctggatgc
     3781 tcgggtatga cccgagggat atatatacaa ccaggatagc tggggcgatg ggacttggtg
     3841 aaaattcgtt agagaatata gagatatttg gagctaaatt agaagacctg tttgggcatg
     3901 acgttaagaa ggttaatacg gtctataatg ttacaaataa gctttcatca cctattttta
     3961 gttttctctc caagagactg gtaaagttta ttaagattta tccagagatt gatgatgata
     4021 aatgcgtaag atgtgaagca tgtcttagaa gttgtcccca gaaggctata gataagtttg
     4081 atgggaggat gaagattaat tataagaaat gtatatcctg ctattgctgt catgaacttt
     4141 gtccacagaa ggctgttatg ataaagaaga acaggttagc tgatatactc tggttagcca
     4201 gagactgaag gtaagtttta aattttagga ggtcttatgg atatcgtaaa gaagataaga
     4261 aatttagcaa aagagaagga tgcaattata cttgcccata actataccag accagagatt
     4321 caggatatag cagattttgt tggagactct ttagaattaa gcaggactgc atccaag
//
LOCUS       DAOTKU010000086         4350 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_17290_length_4350_cov_3.071013, whole genome shotgun sequence.
ACCESSION   DAOTKU010000086 DAOTKU010000000
VERSION     DAOTKU010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4350
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17290_length_4350_cov_3.071013"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1267
                     /gene="hslU"
                     /locus_tag="P5310_03045"
     CDS             <1..1267
                     /gene="hslU"
                     /locus_tag="P5310_03045"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019003750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HRU32372.1"
                     /translation="KLPDEIRKEVSPKNILMIGPTGVGKTEIARRLSQVVDAPFVKVE
                     ATKFTEVGYVGRDVDSMVRDLVEVSVQTVKRREMEKVRAQARLFAEDRLLEYLYPNPQ
                     TYESANPLEMIFQGVARTQTKAVPPEIREKRENARGDLRRGLLDNVIVEIEVESSTMP
                     AFNILSGAGMDDLGINMQNIFDGILPKKKTHRKTTVKEALRILEEEEAKKLIDEESAI
                     REGVIRAEQSGIIFIDEMDKIAGRERGAGPDVSREGVQRDLLPIVEGSTVNTRYGPVR
                     TDFILFIGAGAFHETKPSDLIPELQGRFPIRVELEPLKVEDFVRILTQPENALIKQYT
                     MLLATEGVNLRFTESAIYAIARRSFQLNEELENIGARRLYTVTEKVLEDISFNAPDTG
                     NGEITIDENYIEEKLSGILEERDISKYIL"
     gene            complement(1264..3852)
                     /gene="alaS"
                     /locus_tag="P5310_03050"
     CDS             complement(1264..3852)
                     /gene="alaS"
                     /locus_tag="P5310_03050"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HRU32373.1"
                     /translation="MTSKEIREEFLRFFESKGHLKLASASLIPDDPTVLLTIAGMVPF
                     KKYFLGETPPARRITTVQKCIRTPDIERVGYTRRHLTFFEMLGNFSFGDYFKREAITW
                     SWEFLTESLKLQKEKLWITVYKNDEESRDIWEREIGIPAERIVPMGEDDNFWTMGPVG
                     PCGPCSEVIFDLGPEFGCGKPDCKVGCDCDRYLEVWNLVFTEFDRDKDGHLTPLPRKN
                     IDTGMGLERAAFVIQGVNSVFETDLFRPIMDYIGEITERQYSESRDITYNFNVIADHA
                     RAITFLIGDGVIPGNEGRGYVLRRIIRRAVRYGVKLGLNSPFLHKIVPLVVFIMKDAY
                     PELERESEFITGITKREEENFLSTLERGLDRVEDIIKQEGYISGEEAFRLYDTYGFPL
                     ELTLEIAREKNIGVDIKGFKEIMEESRERTRAGRKKVFLTTLKEDLTSNFIGYDTLEG
                     EGEVLYLTRNNNPVEELGKGERGELVTDVTPFYPESGGQLSDRGIIFSNGTKISVLDV
                     QKKGNAIYHTVEVMEGKVSIGDKVYLKVDGSRRKALARAHTATHILHSVLREKLGDIV
                     KQAGSLVDTDHLRFDFVCFDDIDPTTRDSIEQEINKHIREDLSVKIESLPLETAIKEG
                     AIALFGEKYGDMVRVVGIDSLSKELCGGTHLRGTGEVGAFVIREIYSVGTGVKRVEAL
                     VGEKAIEFVQNERALIEEISDILKSNPQEIIPSIKKREEELESLRKDSQKIQQILAKL
                     LAERIIDNRITLDGIDIVGFLLEDFSIDFLRLIGDRIKEYLPSSIIFLGSKNKDGLRF
                     VIMASKSAVEKGIKAGELMREISKLFSGSGGGREELAQGGGKLIVPFEDGISKLKELV
                     EKKLKN"
     gene            3977..>4350
                     /locus_tag="P5310_03055"
     CDS             3977..>4350
                     /locus_tag="P5310_03055"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HRU32374.1"
                     /translation="MVLSIVALTIATILSLITPRILQSGIDSLTSKDTRNLPYYAGLI
                     VGIATVRGVLTYIQRYLSGLFTEKIGKAIKDELYEHLHHVPVSYFNRMPIGELVSRAS
                     SDVEVVKRFVNNALINVFWIILL"
BASE COUNT         1188 a          969 c          821 g         1372 t
ORIGIN      
        1 gaaactgccg gacgagatca ggaaagaagt ctctcccaag aatatactta tgataggtcc
       61 taccggggta ggtaagactg agatagcaag aagactgtcc caggtggtag atgccccatt
      121 tgtcaaggtt gaagcaacta aatttacaga ggttggatat gttggtagag acgtagattc
      181 tatggtcaga gacctggtcg aggtctctgt tcagactgtg aaaaggagag agatggaaaa
      241 ggtcagagct caggcgaggt tatttgccga ggatagactt cttgagtatc tctatccaaa
      301 ccctcagacc tacgaatctg ctaatcctct ggagatgata tttcagggtg ttgccagaac
      361 ccagactaaa gctgtccctc ctgagatcag agagaagagg gagaatgcta ggggagattt
      421 aagaagaggt ctgcttgaca atgttatagt tgagatagag gtcgaaagtt caactatgcc
      481 tgcctttaat atcctcagtg gggcaggtat ggatgacctg gggataaata tgcagaatat
      541 ctttgatggt atacttccta aaaagaagac gcatagaaag acaactgtta aggaagcgct
      601 gaggattctt gaagaggaag aggcaaagaa acttatagat gaggaaagtg ctataagaga
      661 aggagttatc agggcagagc agtctgggat aatatttata gacgagatgg ataagatagc
      721 gggacgtgag agaggggcag gacctgatgt ctcaagagaa ggggttcaga gggatcttct
      781 tcctatagta gaaggctcca ctgttaatac caggtatgga ccggtaagaa cagattttat
      841 cttatttatc ggagcagggg cattccacga gactaaacct agtgacctta ttccggaatt
      901 gcaggggaga tttcctataa gggtagaatt ggagccattg aaggtggagg attttgtcag
      961 aatactgacg cagccagaga atgcgcttat aaagcagtat acaatgttac ttgccactga
     1021 aggtgttaat ctgaggttta cagagtctgc catctatgct atagccagga ggtcattcca
     1081 gctgaatgaa gagctggaaa atataggggc aaggagatta tacacagtta cagagaaggt
     1141 gctggaagat atatcattta atgctccaga tacaggtaat ggtgaaataa caatagacga
     1201 gaactatata gaggaaaagc tttctggcat tcttgaggaa agagatattt ctaagtacat
     1261 actctaattc ttaagcttct tctctacaag ttccttaagt ttgcttattc catcttcaaa
     1321 tggtacgata agttttcctc cgccctgggc aagctcttcc cgtccacccc cacttccact
     1381 gaagagcttg cttatctctc tcattagttc tcctgccttt atcccttttt ctactgcact
     1441 cttagaagcc ataataacaa atctcagacc atctttattc ttagacccga ggaatattat
     1501 tgaacttggc agatactctt ttatcctgtc acctattagc cttaagaaat cgattgagaa
     1561 atcctctaac agaaatccaa ctatatctat tccatctaac gttattctgt tatctataat
     1621 cctctctgcc agaagtttag caagtatctg ctgaattttc tggctatctt ttcttaaact
     1681 ctctaattct tcctctctct tttttatact ggggataatc tcttgtgggt tacttttgag
     1741 tatatcagat atctcttcta taagagccct ttcgttctgc acaaactcta tcgctttttc
     1801 tccaaccaat gcctcaactc tctttacccc tgttccgaca ctgtatatct ctcttatcac
     1861 aaaggctcct acctctcctg taccccttag atgagtaccc ccacataact ctttgctcaa
     1921 ggaatctata ccgaccaccc ttaccatatc tccatatttt tctccaaata aggctattgc
     1981 tccttcttta atagcggtct ccagaggaag actctctatt tttactgaaa ggtcttctct
     2041 tatatgttta tttatctcct gttctataga atctctcgtt gtagggtcga tatcatcgaa
     2101 gcacacaaag tcaaatctca gatgatctgt gtccacaaga gagcctgcct gtttgactat
     2161 atctccaagc ttctctctca ggactgaatg cagtatatgt gtggctgtat gtgccctggc
     2221 tagagccttc cttctagacc catctacctt tagatagacc ttatccccaa tagatacttt
     2281 tccctccata acttctaccg tatgatatat agcgttaccc tttttctgga catcaaggac
     2341 gctgatctta gtaccattgg agaaaataat ccctctatct gacagttgtc ctccactctc
     2401 tggatagaaa ggagtaacgt cggttactag ctcccctctc tctcccttac caagttcttc
     2461 aactggattg ttgttcctgg tcagatacag gacctcacct tctccctcta gtgtgtcata
     2521 gcctataaaa ttagatgtca gatcctcctt caatgttgtc agaaagacct tctttctacc
     2581 cgctctggta cgttctctgc tttcctccat tatttcttta aagcctttta tatcaacccc
     2641 tatattcttt tccctggcta tctctagggt aagttctagt ggaaatccat acgtatcata
     2701 aagtctgaat gcctcttctc cagagatata tccttcctgt tttataatat cttctaccct
     2761 gtcaagccct ctctctaaag tagataaaaa gttttcctct tctctttttg ttatacctgt
     2821 tataaattcg ctctctcttt ctaattctgg ataggcatcc ttcataatga agacaaccag
     2881 agggactatc ttatggagga aaggtgagtt taagccaagc tttactccat atcttaccgc
     2941 tcttcttatt atacgtctta aaacataccc tctcccctca ttacccggta ttactccatc
     3001 ccctatcaaa aaggttatcg ctcttgcgtg gtctgctatc acattgaagt tataggtaat
     3061 atctctgctc tcactgtatt gacgttctgt tatctctcct atatagtcca taataggccg
     3121 gaacaggtca gtctcaaaga ctgaatttac tccctgaatc acaaatgctg ccctctccag
     3181 acccatcccg gtatcaatat tctttctggg taatggggtc agatggccat ccttatctct
     3241 atcaaattct gtaaatacca gattccacac ttccagatat ctgtcacaat cacaacctac
     3301 cttacagtct ggctttccac atccaaactc tggtccaaga tcgaatatca cctctgagca
     3361 tggtccacag ggacccacgg gacccatagt ccagaaatta tcatcttctc ccatagggac
     3421 tattctctct gccgggattc ctatctctct ttcccatata tccctggatt cctcatcatt
     3481 cttatagaca gttatccaga gtttctcctt ttgaagtttc aggctttcgg ttaaaaattc
     3541 ccatgaccag gttatcgcct ctctcttaaa atagtctcca aaagaaaaat tcccgagcat
     3601 ctcaaagaat gttaaatgcc ttcttgtgta gcccaccctc tctatatccg gggtccttat
     3661 acatttttga acggttgtaa tccttctggc aggaggtgtt tccccaagaa agtatttctt
     3721 aaatggtacc atcccagcta tggtcaacag tacagttggg tcatcaggga ttaaacttgc
     3781 actggcaagt ttaaggtgtc ccttagactc aaaaaatctt aaaaactctt cccttatctc
     3841 tttagatgtc atattttctc tggtctcccg tcaagattta gattgttgat ttattttttt
     3901 tatatgttat catataatct agttgaatac aatggatacg tttctattta aatatgtgtc
     3961 tagatggtgg aaatatttgg tactaagtat agtagctctg acgattgcta ctatacttag
     4021 tcttattaca ccaagaatcc tccagtcagg gattgatagt cttaccagta aagatacgag
     4081 gaaccttcca tattatgctg gtcttattgt tggtatagct actgtcagag gtgttttgac
     4141 ttatatacag agatatctta gtggactttt cactgagaag atcgggaagg ctataaagga
     4201 tgaactttat gaacacctgc atcatgttcc ggtatcctac tttaacagaa tgcccatagg
     4261 agaacttgtc tccagggctt ccagtgatgt agaggtagtt aaaagatttg tcaacaatgc
     4321 ccttataaat gtattttgga ttatccttct
//
LOCUS       DAOTKU010000087         4348 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_17306_length_4348_cov_3.843233, whole genome shotgun sequence.
ACCESSION   DAOTKU010000087 DAOTKU010000000
VERSION     DAOTKU010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4348
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17306_length_4348_cov_3.843233"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..793
                     /locus_tag="P5310_03060"
     CDS             <1..793
                     /locus_tag="P5310_03060"
                     /inference="COORDINATES: protein motif:HMM:NF014674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HRU32375.1"
                     /translation="ELKNINSFRSVERALIYEINRQIDVVSRGGEVIQETRHWDEKNE
                     RTVSMRGKEEAHDYRYFPEPDLVPLEIEDGWIESLKSQIPELPDEKYERYKKLGLTPY
                     EAKLLAHDLELSVFFDRTVDLYNNPKNIANWLLVDVTKELKDRNKVITETSFTPEIMV
                     ELLDLIDQGVISGRIAKDILPELIDRGVSPKKLVEEKGLIQITDISFILSAIDQVLRE
                     NAGVVEEYKKGKEKSFMFLVGQVMKLTRGRANPKLVNELLKKALG"
     gene            898..1902
                     /locus_tag="P5310_03065"
     CDS             898..1902
                     /locus_tag="P5310_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32376.1"
                     /translation="MVGEGNAFKGYNFPVLSRGSIVPSGGFGFRVGPSLFKEDIKRGL
                     LGDLLNMYKTLRSINAEIVISVGDPFNLVFTHLGTKKNIFFISTDQGHSAWVNGFSRI
                     ELELIRRYTLWTFVRDRRTEEFFRERGFRNVSYLGNPLMDTVEEPKFLLEEGITLLPG
                     TRRDSGRNLLLLLDVVREIKKRFKYYVPINNNSEQYIIEALDGRYTLLDWEYGYRVES
                     PVELYIGKGLFSEMVSSSILVIGLSGSGNEQSAGLGKPVVSFYIDGIQFNRKFISEQK
                     KLLGDSLILSDRESVVSIINSLLDNPQEIERRGDAGKIRMGERGAVERISQFITGYKG
                     "
     gene            1899..3209
                     /gene="rlmD"
                     /locus_tag="P5310_03070"
     CDS             1899..3209
                     /gene="rlmD"
                     /locus_tag="P5310_03070"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HRU32377.1"
                     /translation="MKPGDKIILEIEKIIWEGKGLGHYNGQVVMADYVLPGEKVMLRI
                     DDIKRDYATGFPLEIIEPSKERIAPRCRHYYQCGGCSFQHTSYDYQIKLKKSASLEVI
                     SHILGREINGEGPVKSSNPWYYRNKAQFPIHRVKGQVSGGFYSKGTHSIVDLYECPLH
                     PPVFIDILHWAKDEIRKGDLNLRYLYIRQGTSGDIMVTFVVWDRLKKGKEFARELIKT
                     FPEVISVYENINKSSGNVILGDKFYLIEGNKKLEQKINDVIFMISPGSFFQINIHQLK
                     AILDYISDILSPYRGGVFIDGYAGVGTFSLPISRFSRYGYSIEENPIAVEDALDNLKI
                     NKIQNVRVRRGKTELELRQIARNESFDYVILDPPRKGLEKEFSIFLSRLDIGRIIYVS
                     CNPTTLARDIRILESGNFILKHLKGFDLFPQTAHIEYVAVLERR"
     gene            3214..>4348
                     /gene="dnaE"
                     /locus_tag="P5310_03075"
     CDS             3214..>4348
                     /gene="dnaE"
                     /locus_tag="P5310_03075"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HRU32378.1"
                     /translation="MSFVHLHLHSEYSLLDGACRIDDLMEEAVRLEMPACAITDHGNM
                     YGVIKFYKKARSKGIKPIIGCEIYVAPRDMREKNPQEENHHLVLLAMDNAGYRNLTRL
                     VSVAYLEGFYYKPRVDKPTLEKYSDGLIALSSCIQGEISRLILEDDIKGAKATIDWYK
                     NVFPDRFYLELQDHGMEEEAKVNAVMKRLAEEMKVPLVVTNDVHYIRKEDAIYHDILL
                     GVQTNKKINDPDKMRFPTNEFYFKSRDELERLFSDIPDAIANTLEIADRCNIEITFGK
                     YHIPDYPIPEGETTASYLKKLAEEGFRKRYGEHPPEGARERLEKELSVIEMMGFCGYF
                     LIVWDVIHFARTHNIMVGPGRGSAAGSMVSYCLEITNVEPLSMG"
BASE COUNT         1390 a          654 c         1063 g         1241 t
ORIGIN      
        1 ggaacttaag aatataaatt ctttcagatc tgtggagaga gccttaatat atgagatcaa
       61 tagacagata gatgtggttt ctagaggggg agaagttata caggagacca ggcattggga
      121 cgaaaagaat gagcggactg tcagtatgag gggtaaagaa gaggcgcatg attataggta
      181 ttttccagag ccagaccttg ttccattaga gatagaggat gggtggatag aatccctgaa
      241 gtctcaaata ccggagctgc ctgatgagaa atatgagaga tataaaaaac ttggacttac
      301 tccatacgag gcaaaacttc ttgcccatga tctggaactg tctgttttct ttgacaggac
      361 tgtagacctt tataataatc caaagaatat agccaactgg ttactggttg atgtgaccaa
      421 agaacttaag gatagaaata aggttataac tgaaacatca tttacccctg agattatggt
      481 tgaattactt gatcttatag atcagggtgt tataagtgga agaatagcca aagatatact
      541 cccagaactt atagatagag gagtttctcc gaagaaattg gtagaggaga agggtctcat
      601 acagattact gatatatctt ttatactctc tgctatcgac caggtattga gagaaaatgc
      661 tggagttgta gaggagtata aaaagggtaa agagaagtcg tttatgtttc tcgttggaca
      721 ggtaatgaag ttgaccaggg gcagagcaaa tcctaaactt gttaacgaat tgttgaagaa
      781 ggcattgggg taaagattct aatagtcagt aatggatact gtgaagatga tatatcggta
      841 aggttatccc ttgccctgag caggtattat cctcctggtg agataggagt atttcctatg
      901 gtaggggaag gtaatgcatt taaaggttat aactttccag tactatctag aggttctata
      961 gtgccaagtg gaggatttgg attcagggtt ggaccttctc tctttaaaga ggacataaag
     1021 agaggattgc ttggagacct tcttaatatg tataaaacgc tcaggtccat taatgctgag
     1081 atcgttatct cggtaggtga cccctttaat cttgtcttta ctcatcttgg gactaaaaag
     1141 aatatattct ttatatctac tgatcagggg cactctgcct gggtaaatgg tttcagtagg
     1201 atagagttag agttaataag gagatatacc ttatggactt ttgtcaggga caggaggact
     1261 gaagaattct ttagagaaag aggtttcaga aatgtttctt atcttggtaa tcctcttatg
     1321 gatactgtag aagagccaaa gtttcttcta gaagagggga ttactctcct tcctgggacg
     1381 agaagagatt caggtaggaa tctcctcctc ctgcttgatg tagttagaga gataaagaaa
     1441 agatttaaat attatgttcc tataaataat aactcagagc agtatataat tgaagcttta
     1501 gatggtaggt ataccctgtt ggactgggaa tacggctata gggtagagag tccagtagaa
     1561 ctttatatag ggaaggggct cttttcagaa atggtctctt ccagtattct ggtgatagga
     1621 ctttctggga gtggtaacga gcaatcagca ggcttgggaa agcctgtagt gagtttttat
     1681 atagatggga ttcaatttaa tagaaagttt atatctgagc agaagaaact gctgggtgat
     1741 tctcttatac tttcagatag agagtcggtt gtttctataa taaacagtct gttagataat
     1801 cctcaagaga tagagagaag aggagatgca ggtaagatca ggatggggga aagaggagct
     1861 gttgagagga tatcccagtt tataacaggt tataaaggat gaagccagga gataagataa
     1921 tattagagat agaaaagatt atctgggaag ggaagggatt gggacattat aacggtcagg
     1981 tagttatggc tgactatgta cttcccggag aaaaggtgat gcttcgtata gatgatataa
     2041 agagagatta cgctactggt tttcctttag aaataataga gccgtctaaa gagaggatag
     2101 cccccagatg cagacactac tatcagtgtg gtggatgcag ttttcagcat acaagttatg
     2161 attatcagat aaagctaaaa aaatccgcct ctttagaagt tatatcccat atccttggga
     2221 gagagataaa cggagaagga cctgtaaaat cttccaatcc ctggtattac aggaataagg
     2281 ctcaattccc tatacataga gtgaagggac aggtatctgg aggattctat agtaaaggga
     2341 cgcacagtat agtagattta tatgaatgtc ccctgcatcc ccctgttttt atagatatat
     2401 tgcactgggc taaagatgag ataaggaagg gagatttaaa tctcagatat ctctatataa
     2461 gacagggaac ctctggagat attatggtga cttttgtcgt atgggataga ttgaagaaag
     2521 ggaaggaatt tgcaagagag cttatcaaaa ccttccctga ggttataagt gtatatgaaa
     2581 atataaataa atctagtggt aatgtaattc ttggagataa gttctatctt atagagggaa
     2641 ataagaaatt agaacagaag ataaatgatg ttatttttat gatatctccg gggtctttct
     2701 tccagataaa tatccatcaa ttgaaggcta tattagacta catatctgat atactctccc
     2761 cttacagggg tggagttttt attgatggtt atgctggagt tggtacattc agtcttccca
     2821 tctccagatt ttccagatat ggatacagta tagaagaaaa tccgatagct gtggaagacg
     2881 ccctggataa cctcaaaatt aataagatcc agaatgtaag ggtaaggaga ggtaaaacgg
     2941 aactggagct gagacagatt gcgagaaacg agtctttcga ttatgttatt cttgatcctc
     3001 caagaaaggg tctggagaag gagttttcca tatttctctc ccggcttgat atagggagaa
     3061 ttatatacgt atcctgtaac ccaactacac tggcaagaga tataaggatt ctagaatccg
     3121 gaaattttat tcttaagcat ctaaaagggt ttgatctctt tcctcagacc gctcacatag
     3181 aatatgtggc tgttttagag aggaggtgat attatgagct ttgtccatct gcatctgcat
     3241 tcagaatata gtttattaga cggtgcctgc aggatagatg acctcatgga agaggcagta
     3301 agattggaga tgcctgcctg tgcaattact gaccatggaa atatgtatgg tgtaataaaa
     3361 ttctataaaa aagccagatc aaaggggata aaacccataa ttgggtgtga gatatatgtg
     3421 gcacctagag atatgaggga gaagaatcct caggaggaga atcatcatct ggttctcctt
     3481 gccatggata atgctggata cagaaatctg accaggctgg tcagtgtggc atacctggaa
     3541 ggtttttact ataaacccag agtggacaag cctactcttg aaaagtacag tgatggactg
     3601 atagctttat cctcctgcat ccaaggggaa atttcaaggc ttatacttga agatgacatt
     3661 aagggtgcta aggctactat agattggtat aaaaatgtat tccctgatag attttattta
     3721 gagttacagg accatggtat ggaagaggaa gctaaggtta atgcagtgat gaagagactg
     3781 gcagaagaga tgaaagttcc tctggttgtt acaaacgatg tccattacat aagaaaagaa
     3841 gatgctattt accacgatat actccttgga gtccagacga acaagaagat aaatgatcct
     3901 gataagatgc gatttcctac caatgaattt tactttaagt ctagggatga gttagagagg
     3961 cttttttctg atattccgga cgctatagcc aacactctgg aaatagcgga tagatgcaac
     4021 atagagataa cttttggaaa gtatcacata cctgattatc ctatccctga aggagagacc
     4081 actgccagtt atctgaaaaa gttagccgaa gaaggtttta gaaagagata tggggagcat
     4141 cctccggaag gtgcaagaga gaggcttgag aaagagctgt ctgtcattga gatgatggga
     4201 ttctgtggat attttcttat cgtctgggat gttatacact ttgccaggac acacaatatt
     4261 atggttggac caggtagagg aagcgctgct ggtagtatgg tctcttattg tctggagatt
     4321 acaaatgtgg agcctctcag tatgggac
//
LOCUS       DAOTKU010000088         4345 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_17323_length_4345_cov_3.037296, whole genome shotgun sequence.
ACCESSION   DAOTKU010000088 DAOTKU010000000
VERSION     DAOTKU010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4345
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17323_length_4345_cov_3.037296"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1257)
                     /gene="groL"
                     /locus_tag="P5310_03080"
     CDS             complement(<1..1257)
                     /gene="groL"
                     /locus_tag="P5310_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011024996.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HRU32379.1"
                     /translation="MPKLIIFDEEARRKLEKGVNTLADAVKTTLGPKGRNVVLEKKFG
                     SPTITHDGVTVAKEIEVEDPFENVGAQLIKEVATKTADVAGDGTTTATILAQAMIREG
                     LKNVAAGANPMILRRGIEKAVEVCVEELRKMAIRIEDKASIANVATISAADPEIGNLI
                     ADAMERVGKDGVITVEESQTIGTSLEQVEGMQFDRGYVSPYMVTDTERMEAIINDPYI
                     LITDKKISAVNDILPILEKVVQRGRPLVIIAEDVEGEALATLVVNKLRGTLQSLAVKA
                     PGFGDRRKAMLQDIAILTGGEVISDELGIKFENVTIDMLGQARQVKADKDNTTIVEGK
                     GSTEEIKKRIAQIKAQIEQTESDYDREKLQERLAKLSGGVAVIKVGAATETEMKEKKH
                     RIEDALAATRAAVEEGIVPGGGVSLIN"
     gene            complement(1271..1561)
                     /gene="groES"
                     /locus_tag="P5310_03085"
     CDS             complement(1271..1561)
                     /gene="groES"
                     /locus_tag="P5310_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HRU32380.1"
                     /translation="MKLKPLGDRVLVKPIEVEERTKSGIVLPDTAKEKPQQGRVLAVG
                     QGRLLDNGQTVPLQVKEGDRVLYSKYSGTEVKIENEEYLVLSERDILAIIED"
     gene            complement(1697..2755)
                     /gene="proB"
                     /locus_tag="P5310_03090"
     CDS             complement(1697..2755)
                     /gene="proB"
                     /locus_tag="P5310_03090"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HRU32381.1"
                     /translation="MKRIVIKIGTATLCPDDGLDIIFLDDIARQVKYLKEIGISTVIV
                     TSGAIGLGMSMLRDINKNLNLIQKQALAAVGQGKLINYYSTVFERYGLMVAQILLTRE
                     DLANRKRFLNARNTLEELIKLGIIPVINENDTVATEEIKLGDNDQLSALVSTLIDSDL
                     LTILSNVNGLYDKDPHLYKDAKLIKEIRSFDEIPKDIGGPSEKGSGGMITKILAVEIA
                     ARSNIKTIIASGREKDVLIKIINGESVGTLFDIGGERLSKRKVWIAYGVTPKGKIYID
                     QGAYEALAFRNKSLLSQGVKEIDGNFSSGDAVSIISPGGKEFARGIVYYPSDELEELK
                     GKKGYHEVVHRDNLVLLY"
     gene            complement(2752..3471)
                     /gene="lptB"
                     /locus_tag="P5310_03095"
     CDS             complement(2752..3471)
                     /gene="lptB"
                     /locus_tag="P5310_03095"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007958980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HRU32382.1"
                     /translation="MRLRTENLVKRFSQRVVVKGVSIELRSGEVVGLLGPNGAGKTTT
                     FYMITGLLRPNAGKIYLDNQDITTMPIHKRARLGIGYLPQEPSIFRKLTVEENLWTIL
                     QFLPMTDKEREVMCKELMDEFGIAHLKKSMAGALSGGEARRLEVARALLTSPKIILLD
                     EPFLGIDPITVADIQSIIHRLKERGLGVLITDHNVRETLSIVDRAYIISEGELIASGD
                     PLEIANNDKVKQIYLGERFVL"
     gene            complement(3471..>4345)
                     /locus_tag="P5310_03100"
     CDS             complement(3471..>4345)
                     /locus_tag="P5310_03100"
                     /inference="COORDINATES: protein motif:HMM:NF016623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HRU32383.1"
                     /translation="IDTELAQTLNSFFKELNCHIGENWVIDVPSIYSLTWIERRLNNI
                     LAESGYRIDNSKIYLNDKEILELKFNKMPSKGKIAIIIDDVGRTTRLNTILQEIKLPL
                     NISILPKQRETTSMSTAGKKGGWDVLLHLPMEPKEKSWIDSTFIKVGMDSEEVKERID
                     DYLKEMSYVQGVNNHMGSLATSDENIMRTVLGIVREKGLYFIDSLTISNSVGEKVARE
                     IGLERFAKRDIFLDNTDEKEYIRSQIDHLVELSIRKGFAIGIGHLRENTLTTIKDYDW
                     NTKGVELVPLSDVL"
BASE COUNT         1077 a         1073 c          659 g         1536 t
ORIGIN      
        1 attgatcagg gaaactccgc caccaggaac aatcccttct tccactgcag ctctggttgc
       61 agcaagggca tcctctatac ggtgcttctt ctctttcatc tctgtttctg tggctgctcc
      121 aaccttaatt acagctactc caccggatag ttttgccaat ctttcctgga gtttttctct
      181 atcataatca gattctgtct gctcaatctg agccttgatc tgagctatcc tcttctttat
      241 ctcttcagtg ctacctttac cttctactat tgtggtgttg tccttatctg ccttgacctg
      301 tctggcctga ccgagcatat caattgttac gttctcaaac ttgataccaa gctcatcaga
      361 tatcacttct ccgccagtaa gtatcgctat atcctgaagc atagcttttc ttctatcacc
      421 aaatccagga gcctttacag ccagagattg tagagtccct ctcagtttat tcactaccag
      481 tgttgcaaga gcttctcctt ctacatcttc tgctataatt accagtggtc tgcctctctg
      541 tactaccttc tccaggatcg ggagtatatc attgacagca ctgatcttct tgtcagtaat
      601 taagatatag ggatcattta ttatggcttc cattctctct gtatcggtta ccatatatgg
      661 ggaaacataa ccacggtcaa actgcatccc ctctacctgt tccaggcttg tcccgatagt
      721 ctgagattct tctacagtaa taactccgtc ttttcctacc ctttccatag catctgctat
      781 cagatttcca atctcagggt cggctgcaga tattgtcgct acattagcta tagaagcctt
      841 atcctcaatc ctgatcgcca tcttccttaa ttcttctacg catacctcta ccgccttctc
      901 aattcctctt ctcaggatca ttggattagc gccagctgca acgttcttca gtccttctct
      961 aatcatagcc tgggcaagta ttgttgctgt ggtggttcca tctccagcta catcagctgt
     1021 ctttgtcgca acttccttta taagctgagc acccacgttc tcgaatggat cttctacttc
     1081 tatctctttt gctaccgtca caccatcgtg agttattgtc ggagaaccga acttcttctc
     1141 caaaactaca tttcttccct taggacccag tgtagtcttt acagcatctg cgagagtgtt
     1201 tacacctttc tctagcttcc ttctggcttc ttcatcaaat ataattaatt ttggcatata
     1261 ctttcacctc ctaatcttct ataattgcaa ggatatctct ttcactaaga acaagatatt
     1321 cttcattctc aatttttact tctgttcctg aatattttga atataaaact ctatcgccct
     1381 cttttacctg gaggggaaca gtctgaccat tatctagcaa tctaccctgt cctactgcca
     1441 gtactctacc ctgctgaggc ttttctttag ccgtatctgg tagtacaata ccgctttttg
     1501 tcctctcttc tacctctatc ggttttacta acactctgtc acctaaaggc tttagtttca
     1561 tcctatcaac ctcctttttt atatttttag cacttctata tatagattgc taacagctaa
     1621 aagtatacca taatctaaat ttaaatgcaa gagggtgatc agaaattcgc aaacccgaat
     1681 tgaattgcaa atattactaa tacagaagga caagattatc tctgtggact acttcgtgat
     1741 atcccttttt ccccttcaac tcttctaatt catcactggg gtagtatact attcctctgg
     1801 caaattcctt ccctcctggg gatatgattg agactgcatc tcctgaagag aaattcccat
     1861 cgatctcttt aactccctgg gagagaagac ttttattcct aaaagccagt gcttcataag
     1921 ctccctgatc tatataaatc ttcccttttg gagtcactcc gtaggcgatc cagaccttcc
     1981 gtttggataa tctttctcct cctatatcaa acagtgtccc taccgactct ccattgataa
     2041 ttttgataag gacatctttt tcccttccag aagctataat tgttttgata ttacttcttg
     2101 cagctatctc tactgccaat atcttggtaa tcattcctcc tgaacctttt tcagacggac
     2161 cacctatatc ttttggtatc tcatcaaaac tccgtatctc cttaatcaac tttgcatctt
     2221 tatatagatg tgggtcttta tcataaagtc catttacgtt agataatata gtcagcaaat
     2281 cactatctat caaggtcgat accagggcag acaactggtc attatctccc agctttatct
     2341 cctctgttgc cactgtatca ttctcattta ttacaggtat aattcccagc ttaataagct
     2401 cttccagggt attccttgca ttaagaaatc tcttcctgtt tgccaggtct tctcttgtaa
     2461 gcagaatctg tgcaaccata agtccgtatc tttcaaatac agtgctgtaa taatttataa
     2521 gcttaccctg tcccactgct gccagtgcct gcttctggat aagatttaag tttttattta
     2581 tatccctcag catggacatc cccagaccaa ttgctcctga agtgactata acggtagaaa
     2641 ttcctatctc ttttaaatat ttgacttgtc gtgctatatc atccaaaaat atgatgtcca
     2701 gtccgtcatc aggacacagt gtagcggttc ctatttttat tactattctt ttcatagcac
     2761 aaacctctct cccagatata tctgttttac cttatcgtta ttggctatct ctaaagggtc
     2821 tccgctggct atcagctctc cttcgcttat tatatatgct ctgtccacaa ttgaaagtgt
     2881 ttctctaaca ttatggtcag tgattaaaac tcccaacccc ctctctttta acctgtgtat
     2941 tatggattga atatctgcaa cagttatagg gtctattccc aaaaatggtt catcgagaag
     3001 aattattttt ggagaggtca ataatgctct ggcgacttca agtcgtctag cctctccacc
     3061 ggagagtgct ccagccatag actttttaag atgagctatc ccaaattcat ccataagttc
     3121 tttacacata acttctctct ctttatcagt cataggtaaa aactgcagta tagtccataa
     3181 gttctcttcc actgttaatt ttctgaatat agaaggttcc tggggtaaat atcctatccc
     3241 cagtctagcc cgcttatgta tcggcatagt ggtgatgtcc tgattatcca ggtatatctt
     3301 gcctgcattg ggtctaagta atcctgtaat catatagaag gtggtggtct tacctgcccc
     3361 attgggtcct aaaagcccaa ctacctctcc agatcttaac tctatagaga ccccctttac
     3421 taccaccctc tgagaaaatc tttttactaa attttctgtt cttaatctca ttataagacg
     3481 tcgcttaaag ggaccagctc taccccttta gtattccagt catagtcttt tatagttgtc
     3541 agggtatttt ctcttaaatg tcctatccct atcgcaaaac ccttccttat agagagctca
     3601 accaaatggt cgatttgaga ccttatgtac tctttttcat ccgtattatc caggaatata
     3661 tctctttttg caaatctctc cagacctatc tcccttgcta ctttttctcc gacagaattt
     3721 gaaatagtta aactatctat aaagtacaat cctttttctc ttactatacc gagaactgtt
     3781 ctcataatat tttcgtcact agttgccagt gatcccatat gattatttac tccctgcaca
     3841 taagacatct ctttaaggta gtcatctatc ctttccttta cctcttcact atccatacca
     3901 acctttataa aggtactgtc aatccacgat ttttctttcg gttccatagg tagatggagg
     3961 agtacgtccc agcctccttt tttgccagca gtactcatac tggtagtttc tctctgttta
     4021 ggcagtatag aaatattcag tgggagttta atttcctgta agattgtgtt tagtctggta
     4081 gttcttccca catcgtctat tattatagct attttcccct ttgaaggcat cttgttaaat
     4141 ttaagctcaa gaatctcttt atcattcaga tatattttag aattatctat tctgtaacca
     4201 gattcagcca atatattatt taatcttctt tctatccagg taagtgaata tattgatggg
     4261 acatcaataa cccagttttc tcctatatgg cagtttaatt ccttgaagaa agaattgagg
     4321 gtctgtgcca gttcagtatc tatag
//
LOCUS       DAOTKU010000089         4344 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_17333_length_4344_cov_3.054558, whole genome shotgun sequence.
ACCESSION   DAOTKU010000089 DAOTKU010000000
VERSION     DAOTKU010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4344
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17333_length_4344_cov_3.054558"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="P5310_03105"
     CDS             <1..192
                     /locus_tag="P5310_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HRU32384.1"
                     /translation="RNGGGVELQSIGAGAVNQAVKAIAIARGYLAPNGINLVMFPAFT
                     KVEVDGEERTAIKFSVRAI"
     gene            199..1086
                     /gene="ftsY"
                     /locus_tag="P5310_03110"
     CDS             199..1086
                     /gene="ftsY"
                     /locus_tag="P5310_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013403446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HRU32385.1"
                     /translation="MRWFDRLSQGLEKTRALIKRGFSSISSEDLEELEIALLSSDVGP
                     KTTELLLEELKTKAKGSSRDGLSGLLKSSILELFPPSSDIIDRFSQGKWIILVIGVNG
                     SGKTTTIGKLGKILKDRGKSVLFVAGDTFRAAAIEQLEIWGERAGIPVVKHREGADPG
                     AVAFDGIKAAKSRDIDIVMIDTAGRLHTKYNLMEELKKIKRVVEREKENYEIYTLLVI
                     DATMGQNAINQARMFNDALGIDGIAITKLDGTAKGGMVISIASELKIPVVLVGVGEGI
                     DDMQSFNREEFVNALFWES"
     gene            1089..2393
                     /gene="ffh"
                     /locus_tag="P5310_03115"
     CDS             1089..2393
                     /gene="ffh"
                     /locus_tag="P5310_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013401275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HRU32386.1"
                     /translation="MFDILTQRIQNIFDRIKRRGRLSEADIQEVLREIRVTLLEADVN
                     YKVVKDFLERVKAKALQESVLKSVSPGDQILKIIYEELISLLGGEVRAEKHLPTVVML
                     VGLQGAGKTTAAGKLAVYYRKRQGRPLLVACDTRRPAAVQQLEIIGNSINIPVYKEDT
                     DPVTIAVNAVKFAREKVYEPVILDTYGRLHIDNDLMEELKETYERVKPQEVFLVVDAM
                     TGQDAVNIGESFVKFVPLTGIILTKMDGDARGGSALSMRAVNGVPVVFIGTGEKFDAI
                     EPFYPDRMASRILGMGDIKTLVEKVEEAASKERAEKSLEKFTLVDFMEQLKEIRNMGP
                     LDQVMGLIPGFSKLTAKLPVSVDEKKLKRIESIIGSMTIEERENPNIINASRKRRIAS
                     GSGTTVQEVNQLLKQYEQIKNMIKQFSHLEKKGRFPLLGGRI"
     gene            2397..2654
                     /gene="rpsP"
                     /locus_tag="P5310_03120"
     CDS             2397..2654
                     /gene="rpsP"
                     /locus_tag="P5310_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004103399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HRU32387.1"
                     /translation="MVKIRLRRMGKRGRPSYRIIVADIHSPRDGKFIDTIGYYDPIPD
                     PVIINIDLEKAKDWIQKGAQPTDTVKYLLIKAGLDFNALKG"
     gene            2661..2906
                     /locus_tag="P5310_03125"
     CDS             2661..2906
                     /locus_tag="P5310_03125"
                     /inference="COORDINATES: protein motif:HMM:NF024483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HRU32388.1"
                     /translation="MDELYSTVKFLIRCLVDDVDSVDVESEVNGNRVKFIVKVPDEEM
                     GKVLGQGGKTINAVRAVVSTLARSKGKNKVQIVVESV"
     gene            2916..3668
                     /gene="trmD"
                     /locus_tag="P5310_03130"
     CDS             2916..3668
                     /gene="trmD"
                     /locus_tag="P5310_03130"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HRU32389.1"
                     /translation="MSLYIDVVSLFPEAFIGPLNFSIVKRAQQLGYIVVNIFNPRDFT
                     QDRYRKVDDYPYGGGRGMILKPEPVFESVEYIRKLRGEGTAILLSPQGIPLSQSLVED
                     LSRKNSLILICGHYEGVDERVALHLADLEVSIGDYVLSGGEVPAMVIIDAISRMFPQV
                     IDSEVIAEESFYRGIFDYPHYTRPYDFRGYKVPDVLLSGDHKKIELYRRKEALKKTLN
                     RRPELLIRSNLTELDMELLQEIFEEIAKNKLG"
     gene            complement(3660..3938)
                     /locus_tag="P5310_03135"
     CDS             complement(3660..3938)
                     /locus_tag="P5310_03135"
                     /inference="COORDINATES: protein motif:HMM:NF039800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6504 family protein"
                     /protein_id="HRU32390.1"
                     /translation="MEFICEKIKVNYNPQGFDIKSFVWRNREYIIREIISTWQDWKFP
                     DTLSGKRTWLQRYHRNYFVVKTDTDEVFELYLDRKGNRRDWVLLKKLS"
     gene            complement(3992..>4344)
                     /locus_tag="P5310_03140"
     CDS             complement(3992..>4344)
                     /locus_tag="P5310_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32391.1"
                     /translation="ASGNLSGSVYVTYNPGTDNEVALPQAIISSESSTDNFSAEITAE
                     GTNTGDDVVVNCGDTTSEFKIPAGEDFTLKFNIPHFYACRGGSLTELVGTYVLKDKSG
                     NTIDSGAINLTKTE"
BASE COUNT         1395 a          664 c         1058 g         1227 t
ORIGIN      
        1 agaaacggtg gaggagttga actacaatca atcggtgcag gggcggtaaa tcaggcggtt
       61 aaagctatag ctatagctag aggttattta gctcctaatg gaattaatct ggtaatgttt
      121 cctgcgttta ccaaggttga ggttgatgga gaggagagaa ctgctataaa gttttctgtc
      181 cgagccatat aagataaaat gagatggttt gacaggttaa gccagggatt agagaagacc
      241 agggcattaa taaagagagg atttagcagt atatcatcgg aagacttaga agagctagaa
      301 atagctcttc taagttctga tgttgggcca aagactactg aattactatt agaagaactt
      361 aagacgaaag ctaaaggttc ttccagggat ggactatctg gtctccttaa gagtagtata
      421 ctggaacttt tccctccctc gtctgatatt atagatagat tttcacaggg gaaatggatt
      481 atactggtta taggagtaaa tggaagcggt aaaactacca ccataggtaa attgggtaag
      541 attcttaaag ataggggaaa atctgttcta tttgttgcag gcgatacatt cagggcggct
      601 gctatagaac aattagaaat ctggggagaa agagcaggga ttcctgttgt aaaacataga
      661 gaaggggctg acccaggggc ggttgctttt gatggtataa aggctgctaa atcgagggat
      721 atagatatag tgatgataga tactgctggt agacttcata caaagtataa tcttatggaa
      781 gagctgaaga agataaaaag agttgtggag agagaaaaag agaactatga gatttatact
      841 ttactggtaa ttgatgcgac tatgggacag aatgctataa atcaggcacg tatgtttaat
      901 gatgctttgg ggatagatgg gatagctatc accaaacttg atggaaccgc aaaaggtggt
      961 atggttataa gtatagcctc agagttgaag ataccagttg ttctggttgg agttggagaa
     1021 ggaattgacg atatgcagag ttttaataga gaagagtttg tcaatgcttt attctgggag
     1081 agttaattat gtttgatatc cttactcagc gtatacagaa tatttttgac agaattaaga
     1141 gacggggaag actcagtgag gcagacatac aggaagtttt gagagagata agagtgactc
     1201 tccttgaggc agatgtaaac tataaagtag taaaagattt tctcgaaaga gttaaggcta
     1261 aggctcttca ggaatctgta ttgaagagcg tatctcctgg cgatcagatt ttgaagatta
     1321 tatatgaaga gcttatatct ttacttggag gggaagtcag ggcagagaaa catctgccca
     1381 ccgtagttat gctggtaggg cttcagggag caggtaagac caccgctgcc ggtaagctgg
     1441 cggtgtatta taggaaaagg cagggtcgtc ccctactggt tgcctgtgat accagaagac
     1501 cagcagctgt tcagcagtta gaaataatag ggaatagtat aaatatcccc gtttataaag
     1561 aagatactga cccagttacc atagctgtaa atgcagttaa gtttgccaga gaaaaggtct
     1621 atgagcctgt tatacttgat acatatggaa gacttcatat agataatgac ctgatggaag
     1681 aattaaaaga gacatacgaa agagttaaac ctcaggaggt ctttcttgta gttgatgcta
     1741 tgacaggaca ggatgcggta aacataggag aatcgttcgt taagtttgtc ccccttacgg
     1801 gaataattct caccaaaatg gatggggatg ccaggggtgg ttctgctctc tctatgaggg
     1861 ctgttaatgg agtcccggtg gtatttattg ggacaggaga aaagtttgat gctatagaac
     1921 ctttttaccc agatagaatg gcaagtagaa tccttggtat gggggatata aagacgctgg
     1981 tagaaaaggt tgaagaggca gcttctaaag aaagggcaga aaaatctctg gaaaaattca
     2041 cattggttga ttttatggag caacttaaag agatcagaaa tatgggacct ctagaccagg
     2101 ttatggggct tatccctggg ttttctaagt tgactgccaa attacccgta tctgtagacg
     2161 aaaagaaact taaaaggatt gagtcaatta taggttctat gactatagaa gagagagaaa
     2221 atccaaatat aattaatgcg agcaggaaga gaagaatcgc cagtggtagc ggaacaacag
     2281 tgcaggaagt aaatcagctg cttaaacagt atgaacagat aaaaaatatg attaagcaat
     2341 tctctcattt agagaagaag ggaagatttc cattattagg aggtagaata tagaatatgg
     2401 taaagataag gcttagaaga atgggtaagc ggggcagacc aagttacagg attatagtgg
     2461 cagatataca tagccctaga gacggtaagt tcatagatac aataggatat tatgatccga
     2521 ttcctgatcc agtgattata aatatcgatt tggagaaggc taaagactgg attcagaagg
     2581 gagctcagcc taccgataca gtgaaatatc tgcttataaa agcgggatta gacttcaatg
     2641 ctctaaaggg gtaatggaag atggatgagc tttatagtac ggtgaagttt cttataagat
     2701 gccttgttga tgatgttgat agtgtggatg tagaatctga agttaatggt aacagggtaa
     2761 agtttatagt gaaagtccct gatgaagaga tggggaaagt attggggcaa ggggggaaaa
     2821 ctataaatgc tgtgagagca gttgtcagta ctttggctcg tagtaagggg aagaacaagg
     2881 tccagatagt ggtggagtct gtctgattta tagatatgtc tctttatata gatgttgttt
     2941 ctcttttccc cgaagccttt attggtcctc taaattttag catagtaaag agggctcaac
     3001 agttaggtta tatagtggta aatatattta accccagaga ttttactcaa gacaggtata
     3061 gaaaagttga tgactatcca tatgggggag gcagaggtat gatacttaag cccgaacctg
     3121 tttttgagag tgtcgagtat ataagaaaac ttagagggga agggacagca atacttcttt
     3181 cccctcaagg tataccactc agccaatctc tggtagagga tctgagcaga aaaaactctt
     3241 tgatacttat atgtggtcat tatgaggggg tagatgaaag ggtagctctt catctggcag
     3301 atttagaggt atctataggg gattatgtcc tctctggtgg agaggtcccg gctatggtaa
     3361 ttatagatgc tatctccagg atgttccctc aggttattga tagtgaagtg atcgctgaag
     3421 agtcttttta taggggaatt ttcgattatc cccattatac tagaccttac gattttagag
     3481 gatataaagt cccggatgtt ttactgagtg gagaccacaa aaagatagaa ctttatagaa
     3541 ggaaagaggc attaaagaaa acacttaata gaagacctga attgcttata aggtctaatc
     3601 tgacagagct tgatatggaa cttttacagg agatttttga agagatagca aagaataaac
     3661 taggataact tttttaaaag gacccaatcc cttctattac cctttctatc cagatatagt
     3721 tcaaaaactt catccgtgtc ggtttttacg acaaagtagt ttctgtgata cctctgtagc
     3781 caggtgcgtt taccagaaag agtatctgga aatttccagt cttgccaggt agaaataatt
     3841 tccctgatga tatactctct attcctccag acaaagctct ttatatcgaa tccttgagga
     3901 ttatagttaa ccttgatctt ttcacatata aattccatag atggtaataa aagcccagaa
     3961 gattataatt cttccgggct ttttcttcta attactctgt ttttgtcaga ttaatagcac
     4021 cggaatctat agtattaccg gacttatcct taagtacata tgttccaacc agttcagtta
     4081 ggcttccacc tctgcaggca taaaaatggg gtatattgaa cttaagagta aaatcttcac
     4141 cggctgggat tttaaattct gaagttgtat caccgcaatt tacaaccacg tcatctcctg
     4201 tattagtccc ctcagccgta atctctgctg aaaagttatc tgttgaactc tcactactaa
     4261 taatagcctg tggaagtgca acctcattat cagtaccagg attataggtc acatataccg
     4321 aaccggatag attcccggat gcat
//
LOCUS       DAOTKU010000090         4306 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_17507_length_4306_cov_3.754411, whole genome shotgun sequence.
ACCESSION   DAOTKU010000090 DAOTKU010000000
VERSION     DAOTKU010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4306
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17507_length_4306_cov_3.754411"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="P5310_03145"
     CDS             complement(<1..264)
                     /locus_tag="P5310_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010689036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRU32392.1"
                     /translation="MDNRELTPKEIVRELDRYIIGQDQAKRAVAIAIRNRIRRMKLPE
                     ELRREVSPKNILMIGPTGVGKTEIARRLSQVVDAPFVKVEATKF"
     gene            complement(295..1770)
                     /locus_tag="P5310_03150"
     CDS             complement(295..1770)
                     /locus_tag="P5310_03150"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase family protein"
                     /protein_id="HRU32393.1"
                     /translation="MDNRVFLAFDLGAESGRAVVGSLNENRITLREVHRFMNRPVRIL
                     GRLYWNVTEMFQELKQGLISGVREFPDIESIGIDTWGVDFGFISTNNELVGLPVCYRD
                     SRTNGMPEKVFEIIPREKLYQLTGIQIMQINSIFQLYSMKLENSPLFNSIHKLLFMPD
                     LFNFMFTGKVSTEFSIATTSQLYDPINNRWSRDIFDALDLPMEIMPEILDTGADLGDI
                     YPDIREELGVRGITIVAPAEHDTGSAVAAVPAEGDNWAYISSGTWSLMGVEVKEPIIT
                     KESMDANFTNEGGINRTFRFLKNITGLWLLQGCRRSWEREGDNLSYNEIVRLAEESKP
                     FKFFIDPDDSSFLNPPDMPSAIKDFCIKTGQGVPETRGEIARCIFESLAFRYKDVFET
                     LKSLIKKDIDTLHIVGGGSQNTLLSQFTADALEVPVYTGPVEATAMGNIMVQAMAKGA
                     IKDLEEGRRLIKNSFETVVYYPKDVNLWEKEYERFKNVIIR"
     gene            1863..3056
                     /gene="rhaI"
                     /locus_tag="P5310_03155"
     CDS             1863..3056
                     /gene="rhaI"
                     /locus_tag="P5310_03155"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HRU32394.1"
                     /translation="MKNYEKRYELLKESLGERGISIDEVKEKLKSLRIETPSWGYGDS
                     GTRFYVFKQKGAARDVWEKIQDAAQVHKLTGVCPTVALHIPWDYTENWEELLEYTKSL
                     GVKPGAINPNLFQDDDYKLGSLCNPDKRIREKAIAHILECIDIASKLGSRDISLWISD
                     GTNHPGQDDIKERKHRLEESLMEVYKNLGNNMRLLIEYKFFEPAFYLTDVGDWGMAYL
                     LSTKLGPKAMVLVDLGHHPLGTNIEQIVAYLIDEKRLGGFHFNNKKYADDDLTVGSIN
                     PYELFLIYNELVAAEYDGMKLDVAYMIDQSHNIKQKIEEMLQSVENIQKAYAKALIVD
                     RKFLKKYQLEGDIVSAEKILTDAFETDVMPIIYKAREELGVPLDPISSLRDSGYLEKI
                     RKERV"
     gene            complement(3081..3461)
                     /locus_tag="P5310_03160"
     CDS             complement(3081..3461)
                     /locus_tag="P5310_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRU32395.1"
                     /translation="MMKKVISTDKAPKAIGPYSQAIDAGNFIFISGQIPVDPVTGEIK
                     SDIKGATVQIMENIKAILENAGLNLSSVIKTTVFLTDMEDFKEFNSVYGSFFEKDPPA
                     RSTVAVEELPRGVRIEIEAVAYKG"
     gene            3546..4070
                     /locus_tag="P5310_03165"
     CDS             3546..4070
                     /locus_tag="P5310_03165"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRU32396.1"
                     /translation="MQIEELVRSTLKFEGRIIKVKVDEVRIPDGSIVTREVVVHRGAV
                     GILPIMDNKVFLVRQYRHPAGEILWEIPAGIFGESETPEECAIRELREETGLFPLNLV
                     RLGEVFVSPGCSTEKIYLFYADKFKSDPLPKDEDENIEVGEFDIDTFRDMIEEGKIRD
                     GKTLASFCLYLLKR"
     gene            4067..>4306
                     /locus_tag="P5310_03170"
     CDS             4067..>4306
                     /locus_tag="P5310_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00375 family protein"
                     /protein_id="HRU32397.1"
                     /translation="MKIFCDLHIHIGSTSRGEAVKVTGAKNLTFGNIARECKERKGVQ
                     VVGIVDCASPVVLKDIDELIESEEMVELQDGGLLYR"
BASE COUNT         1322 a          884 c          863 g         1237 t
ORIGIN      
        1 aaatttagtt gcttcaacct tgacaaatgg ggcatctacc acctgagaca atcttcttgc
       61 tatctcagtc ttacctactc cagtaggacc tatcataagt atattcttgg gggagacctc
      121 tcttctgagc tcttctggca gtttcatcct ccgtattcta ttccttatag ctatagcaac
      181 agctcttttc gcctggtcct gtccaattat atatctatca agttctctga ctatctcttt
      241 gggtgtaagt tctctattgt ccaaatcaat tcacttccct tttaataaat tctctcatct
      301 tattataaca tttttaaatc tctcgtattc tttttcccac aggttgacat cctttggata
      361 gtacactacc gtctcaaaag aattttttat caatcttctc ccctcttcca gatccttaat
      421 agcaccctta gccatagcct gaaccataat atttcccata gcggtagctt ccaccggacc
      481 agtatatacc ggtacttcca acgcatcagc tgtaaactgg gataatagag tattctgaga
      541 accaccaccc actatatgca gggtatctat atccttcttg ataagactct tcagtgtctc
      601 aaaaacatcc ttatatctaa atgcaaggct ctcaaagatg catctcgcga tctcccccct
      661 tgtctcaggg actccctgac ctgtctttat gcagaaatct tttatagcag atggcatatc
      721 aggaggattc aagaaggaag aatcatcagg gtctataaag aacttaaaag gcttggactc
      781 ttcagctaac ctgactatct cattatagga taaattgtcc ccttccctct cccaggacct
      841 tctgcatccc tggagaagcc atagacccgt tatattttta aggaatctaa atgtcctgtt
      901 aatccctcct tcattggtaa agttggcatc catagattct ttagtaatta taggttcctt
      961 tacctctaca cccattaaag accatgttcc ggaactgata taagcccaat tgtctccctc
     1021 agctggaaca gcagctacag ctgacccagt atcatgctcg gctggtgcaa caatagttat
     1081 acctcttaca ccaagttctt cccttatatc aggatatatg tctccaagat ctgcccctgt
     1141 atccaaaatc tcaggcataa tctccatggg tagatcaagg gcatcgaaaa tatctctact
     1201 ccacctgtta ttaatagggt cgtataactg agaagtggtg gcaatagaaa attcagtact
     1261 cactttacct gtaaacataa agttaaacag gtctggcata aaaaggagct tatgtataga
     1321 attgaaaagg ggggaattct caagtttcat actgtagagc tgaaagatag aatttatctg
     1381 cataatctga atcccggtca actgatacag tttctctctc ggtattattt caaatacctt
     1441 ctctggcata ccatttgtcc tggagtccct ataacatacc ggcaatccca ccagttcatt
     1501 gtttgtactt ataaaaccaa agtccacacc ccaggtatct attcctatac tctctatatc
     1561 cggaaattct ctgaccccac ttatcaatcc ctgttttaat tcctgaaaca tctcagttac
     1621 attccagtag agtcttccca atattctcac cggtctattc ataaacctgt gaacctctct
     1681 gagtgttatc ctgttctcat ttaaagaacc gaccactgcc cgaccactct ccgctccaag
     1741 atcaaaggcg aggaatactc tgttatccat agatatctcc ctccttgttt tttattcggt
     1801 atcttaatta taacagatta tggtaatatt agtattaaat tatatgctgg gaggataaag
     1861 atatgaagaa ttatgagaaa aggtatgaac tgctgaagga atctctagga gagagaggaa
     1921 tatccataga tgaggtaaaa gaaaagttaa agagtctcag gatagagacc ccaagctggg
     1981 gatacggaga ttctggaacc agattttatg tgttcaaaca aaaaggagcc gccagggatg
     2041 tatgggagaa aatccaggat gcagcccagg ttcataaact cactggagtc tgtcccactg
     2101 tcgccttaca tataccctgg gactatacag agaactggga agaactgctt gagtatacga
     2161 aatcacttgg ggtaaaaccc ggagcaataa atccgaatct atttcaggac gatgactata
     2221 aattgggaag tctctgtaac cctgataaaa ggataaggga aaaggctata gcccatatcc
     2281 tggaatgcat tgacatcgcc agtaaattag gctccagaga cataagtcta tggatatctg
     2341 atgggacaaa ccatccaggt caggatgata taaaggagag gaaacatcga ctggaagaat
     2401 ccttgatgga ggtttacaag aacctgggta ataatatgag acttctcata gagtataaat
     2461 tctttgaacc tgccttttac ctgaccgacg ttggagactg gggaatggct tatctactct
     2521 caacaaaatt gggaccaaag gcgatggtgt tggtagacct tggacatcat cctctgggga
     2581 caaacataga acagatagtt gcctatctaa tagatgagaa aagattgggt ggattccatt
     2641 ttaacaacaa aaaatatgcg gatgatgacc ttacagttgg ttcaataaac ccctatgaac
     2701 tgttccttat ctataatgag ctggttgccg cagagtatga cgggatgaaa ctggatgtag
     2761 cttatatgat agatcagagt cataatataa agcagaagat agaagagatg ctgcagagtg
     2821 tagagaatat tcaaaaagcc tatgctaagg ctctgatagt ggatagaaaa ttcctgaaga
     2881 agtatcaact tgaaggagat atagtcagtg cagaaaagat tctcacagat gcattcgaga
     2941 cagatgttat gccaataata tacaaagcca gagaagaact aggagtaccc cttgatccga
     3001 tatcctcatt aagagatagt ggatatctgg aaaagataag aaaggaaagg gtttagagag
     3061 cggggtctac ccgctcttat ttacccctta taagccaccg cctctatctc tatcctgact
     3121 cctctgggaa gttcctctac cgctaccgta cttctggcag gtggatcttt ttcaaaaaag
     3181 cttccataaa cagaattaaa ttctttgaaa tcttccatat cagtaagaaa aacagtggtc
     3241 tttattactg aacttagatt taacccggca ttttccagta tggcttttat attctccatt
     3301 atctgaactg tagctccttt tatatctgac ttgatttcac ctgtaaccgg atcaactggg
     3361 atctgtcctg atataaatat aaaatttcca gcatcaatag cctgagaata cggtccgata
     3421 gcctttggtg ccttatcggt cgatatcact tttttcatca ttcatcctcc tgcaagatat
     3481 ttttagttat tttatcactt tccagaatat ttgtataatc ttttcttttg ctgtataatt
     3541 aagctatgca gatagaagag cttgtaaggt caacactcaa atttgaagga cggattataa
     3601 aagtaaaggt agatgaggta agaattcctg atggaagcat agtaaccaga gaggttgtcg
     3661 ttcacagagg agcggtagga atcctcccca tcatggacaa taaggtattc ctggtaagac
     3721 agtatagaca tccagctgga gaaatcctgt gggagatacc tgctggaata ttcggagaaa
     3781 gtgaaacccc tgaggaatgt gccataagag agttgagaga agaaacggga ctatttccat
     3841 taaatttggt aaggttgggg gaagtatttg tatctcctgg atgcagtacg gaaaagatat
     3901 acctcttcta tgcagacaag tttaagtcag acccacttcc aaaggatgag gatgaaaata
     3961 tagaggtggg agaattcgat atagatacct tcagagatat gatagaagag ggaaagataa
     4021 gagatggtaa aacgttagcc tctttctgtc tctacctgtt aaagagatga agatattctg
     4081 cgaccttcat atacacatag gaagtacatc aaggggagag gcggttaagg taacaggggc
     4141 gaagaacctt acctttggca atattgccag ggagtgtaaa gagagaaagg gggtacaggt
     4201 cgttggtata gtggactgtg catccccggt cgttctaaaa gatatagatg agcttataga
     4261 atcagaggag atggttgaac ttcaagatgg agggctctta tacagg
//
LOCUS       DAOTKU010000091         4306 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_17508_length_4306_cov_3.413550, whole genome shotgun sequence.
ACCESSION   DAOTKU010000091 DAOTKU010000000
VERSION     DAOTKU010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4306
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17508_length_4306_cov_3.413550"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="P5310_03175"
     CDS             <1..541
                     /locus_tag="P5310_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32398.1"
                     /translation="NSPRALGYKRGIIESIPDRLTIVISDRPWAKRFVDYTPGKRVIL
                     EPLPGAKRRDFQVRKERRESLLKEYFSTNLTLFYASIYLFYFPYPLLSLKECLTDMLK
                     YKWTIREEDLNGLIVGLKDRDGRFLGLGRIIEMTGNNLLLETPVNFIREIRFIELSKI
                     RVDGQYKETGKLLIFQKEE"
     gene            545..1546
                     /gene="gap"
                     /locus_tag="P5310_03180"
     CDS             545..1546
                     /gene="gap"
                     /locus_tag="P5310_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HRU32399.1"
                     /translation="MKVGINGFGRIGRQVFKALIDRYPDIEVVAINDITDITTNVHLL
                     KYDSNYGRFPGEVKVDGNDMVVNSRKVKVFAEKDPANLPWKDLGVDLVVESTGLFTDA
                     DKASAHIKGGAKKVIITAPAKGEDITIVLGVNEDKYDPGKHHIISNASCTTNSLAPVS
                     KILNDKFHIISGLMTTTHSYTNDQRILDLPHKDLRRARAAALNIIPTTTGAAKAIGAV
                     IPELKGKMNGVALRVPTPTVSITDLVCVVEKEVTVEEVNKAFEEAASGRMKGILDITY
                     EPLVSMDFKGTTYSTVVDGLSTMAIGNLVKVLAWYDNEWGYSCRVADLVAFVRDKGI"
     gene            1559..2758
                     /locus_tag="P5310_03185"
     CDS             1559..2758
                     /locus_tag="P5310_03185"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020155097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HRU32400.1"
                     /translation="MNKLTIKDIDLKGKKVLTRVDFNVPLADGKVADDTRIRAALPTI
                     NYMREKGAKVILVTHLGRPKGVTEGLRLDPVAQRLKELGVPVKKLNDCIGDEVKKAVS
                     EMKEGEVILLENVRFYPEEEKNDPKFAQELASLADIYVNDAFGTAHRAHASTEGVARI
                     LPAVAGFLMEKEITFFSKVLESPERPFVAVLGGAKVSDKIGVIKNLLDKVDIILIGGG
                     MAYTFLKSLGYEVGKSICEIDKVSLAKELLNLAKEKKVELILPVDVVVADKFAPDANT
                     KIVPVSQIPPDWQGLDIGPETRQLFSQKVSQAKTIVWNGPLGVFEMPAFSEGTKAVGI
                     AIANTDALKVVGGGETAEAVEKFGLTDKMTHVSTGGGASLEFLEGKVLPGVAILDDSE
                     SIKRKLK"
     gene            2755..3504
                     /gene="tpiA"
                     /locus_tag="P5310_03190"
     CDS             2755..3504
                     /gene="tpiA"
                     /locus_tag="P5310_03190"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013638193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HRU32401.1"
                     /translation="MRRRLIAGNWKMNGTVSDAIALVQRLKILISDIQDRDVAVCPPF
                     TLLDTVSKMIIDTRIFLGAQDMFWEEKGAFTGEISPLMLKEFNCTFVILGHSERRWII
                     GETDEFISKKVDSAIRNGLTPILCVGERLEDREADRTRDVVISQLNGSLSLIKDPESL
                     VIAYEPVWAIGTGKPAYPEDAVEVISMIRDWYRERFGKEKGESVRILYGGSVTPSNID
                     SFMKEEEIDGALVGGASLNADSFARIVKYLM"
     gene            3516..3734
                     /gene="secG"
                     /locus_tag="P5310_03195"
     CDS             3516..3734
                     /gene="secG"
                     /locus_tag="P5310_03195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HRU32402.1"
                     /translation="MKTTFMIIQLVIAIVLSLVILLEPPRGEGLGAIGGSAKLFHVGK
                     EKERRLDMIVAIAGGVFFVLAGILAWAM"
     gene            complement(3803..>4306)
                     /locus_tag="P5310_03200"
     CDS             complement(3803..>4306)
                     /locus_tag="P5310_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003542608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HRU32403.1"
                     /translation="PVRIAGRCTVFAESDMIHKQQLGHRQEDILFGLCEALVRNYLSN
                     VGRGKEIRPPILFQGGVAANKGIKVALERALGVEIIIPPYFSLMGAIGAALLAQEYGI
                     KRTRFKGFETADQNITTRGFECKGCSNLCEITNVLIEGKLIARLGGRCGKWENLSEED
                     LSLTIDS"
BASE COUNT         1320 a          742 c         1042 g         1202 t
ORIGIN      
        1 aaattctccc agggctcttg gttacaagag aggtataatt gagtctatac ctgatagact
       61 gacaatagtc atatctgata gaccctgggc taaaagattt gtcgattata ccccggggaa
      121 aagagtgata ttagaacctc taccaggggc aaaacggaga gatttccagg ttagaaaaga
      181 acgaagagag tctttattaa aagaatactt ttctaccaat ctcacccttt tttatgcaag
      241 catatatttg ttctattttc cttacccgct tctctcttta aaggagtgtt tgactgatat
      301 gctaaaatat aagtggacta tcagagaaga agaccttaac gggttaattg tggggttaaa
      361 ggatagggat ggcaggtttt taggtctggg aaggataatt gagatgactg gaaataatct
      421 tttgttagag actcctgtaa attttataag agagataaga tttatagaat tatcgaagat
      481 aagggttgat ggtcaatata aagaaacagg aaagttatta atttttcaaa aggaggaatg
      541 aagaatgaaa gtaggtataa acggttttgg cagaatagga agacaggttt tcaaagcact
      601 tatagataga tatccagata tagaggtagt agctataaat gatataacag atataacaac
      661 aaatgtacat ttactcaagt acgattctaa ctatggaagg ttccctggtg aagtaaaggt
      721 agatggaaat gatatggtgg ttaattccag gaaggtaaag gtttttgctg aaaaagaccc
      781 ggcaaacctc ccctggaaag atctgggtgt ggatctggtt gtagaatcta ccggactctt
      841 tacagatgcg gataaagctt cagcccatat taaaggagga gcaaagaagg ttattattac
      901 agctcctgct aagggggaag atataacaat agtccttggg gtaaatgagg acaagtatga
      961 tccgggtaaa catcatataa tatcaaacgc ttcctgcacc accaacagtc ttgctcctgt
     1021 ttctaagatt ttaaacgaca aattccatat aataagcggt cttatgacca ccacacattc
     1081 ttatacgaat gatcagagaa ttctggattt gcctcataaa gacctcagga gggcgagagc
     1141 agctgcattg aatattatcc caaccactac cggtgcggca aaagcaattg gtgcagtgat
     1201 tccagaatta aaaggtaaaa tgaacggagt ggcattgaga gtaccaactc caacagtttc
     1261 aattacagac ctggtatgtg tggtggagaa agaagtaact gttgaagaag tgaataaggc
     1321 ttttgaagag gcagcttctg gtcgtatgaa gggaattctg gatattacct atgaacctct
     1381 tgtctctatg gatttcaagg ggacaacata ctccacagtg gtcgatggac tttccactat
     1441 ggctatagga aatctggtaa aggttcttgc ctggtatgat aatgagtggg gttattcttg
     1501 ccgggtagct gacctggtgg cattcgtaag ggataaggga atctaaggag taaccgttat
     1561 gaataaactt actattaaag atatagacct gaaagggaaa aaggttctga ccagggtgga
     1621 ctttaacgtc cccctggctg atggaaaggt ggctgatgat accagaataa gggctgccct
     1681 tcccaccata aattatatga gagagaaggg ggctaaagtt atccttgtta ctcatcttgg
     1741 aagacccaag ggtgttaccg agggactgag attggaccct gtagcccaga gactaaaaga
     1801 gcttggagtt ccagtgaaaa aactaaatga ttgtatagga gacgaagtaa agaaagctgt
     1861 atcagagatg aaagaaggag aggtaattct tcttgagaat gtaaggttct atcctgagga
     1921 agagaaaaac gatccaaaat tcgcccagga attggcaagc cttgcagaca tctatgtaaa
     1981 cgatgccttt ggaactgcac acagggctca tgcatctact gaaggggttg ctagaatact
     2041 ccccgcagtt gcaggatttc ttatggaaaa agagattacc ttcttttcca aggtgctgga
     2101 atctccggaa agaccatttg tagcggtatt gggtggggca aaggtatctg acaagatcgg
     2161 ggttattaag aatcttctgg acaaagttga cattatcctg ataggtggag gtatggctta
     2221 taccttctta aaatccctgg gttatgaggt gggtaagtct atctgtgaga tagacaaagt
     2281 ctcactggca aaagaacttc tgaatttagc taaggaaaag aaggtagaac ttatacttcc
     2341 agtagatgtg gttgtcgctg ataagtttgc tcctgacgcc aatacaaaaa ttgtacctgt
     2401 atctcagata ccacctgact ggcaaggtct ggatatagga ccagagacaa gacagctctt
     2461 ttctcaaaaa gtgtctcagg ctaagactat agtatggaat ggacctctgg gggtattcga
     2521 gatgccggct tttagtgaag gcacaaaggc tgttggaatt gccatagcaa atactgatgc
     2581 actgaaggta gttggtgggg gagaaactgc agaggcagtg gaaaagtttg gacttacaga
     2641 taagatgacc catgtctcca ctggtggcgg tgcaagttta gaattcctgg agggtaaggt
     2701 tctcccagga gtagcaatac tggatgatag tgagagtata aagaggaagc ttaaatgaga
     2761 agaaggctta tcgcggggaa ctggaagatg aatggtactg tatcagatgc tattgctctg
     2821 gtacagcgcc tgaagatatt aatttctgac attcaggata gggatgtcgc tgtatgccca
     2881 ccatttactc ttttagatac ggtatcaaag atgataattg atacaaggat atttcttggt
     2941 gctcaggata tgttttggga agaaaaggga gcttttaccg gtgaaatatc cccattgatg
     3001 cttaaagagt ttaactgtac atttgttatc ctcggacatt ctgaaagacg ctggattata
     3061 ggggagacag acgaatttat atctaagaag gttgattctg ctatcagaaa tgggcttact
     3121 cctatcctct gtgtcgggga acgtctggaa gatagagagg ctgatagaac cagggatgtg
     3181 gtgatctcac agttaaatgg aagcctctcc ttaattaagg accctgaatc tctggttatt
     3241 gcatatgagc cagtgtgggc tatcggtacc gggaaacctg cctatcctga agatgcagta
     3301 gaagttatat ctatgataag agactggtat cgagagcggt ttgggaaaga gaagggagaa
     3361 agtgtaagga ttctctatgg aggaagtgta actccttcca acatagatag ctttatgaaa
     3421 gaagaagaga tagatggtgc actggttggt ggggcaagtt taaatgcgga tagttttgct
     3481 agaatagtga agtatttgat gtagatggag gtcttatgaa gacaacattt atgataatac
     3541 agcttgtaat agccatagtg ttaagtctgg tgattctatt agaacctcca agaggagagg
     3601 gtctgggagc tataggtggt tctgctaaac tttttcacgt tggaaaagaa aaagaaagac
     3661 ggctggatat gattgtggct attgcgggtg gggtattttt tgtactggct ggaatcctgg
     3721 cttgggctat gtagacaggt agacagataa aggaggcgga attccgcctc cttatttttt
     3781 tgtttttata catactgata cactaactat ctatagtcaa agataaatct tcctcactta
     3841 agttctccca ctttccacat cttcccccca acctcgctat aagtttcccc tctataagga
     3901 cattggtaat ctcacaaaga ttagagcatc ctttgcattc aaatcctctt gtggtaatat
     3961 tctggtcagc tgtctcaaac cccttaaacc ttgtcctttt tattccatat tcctgggcta
     4021 atagtgcagc ccctatagca cccataagag aaaaatatgg tggtattata atctctaccc
     4081 ccagggctct ttcaagagct acctttatcc ccttattagc cgctacgcca ccttgaaaga
     4141 gtataggtgg tcttatttcc tttcctcttc cgacattact caagtaattt cttaccagag
     4201 cttcacagag accaaacagg atatcttctt gcctgtgacc aagttgttgt ttgtgtatca
     4261 tatctgactc tgcaaaaaca gtgcatctac cagctattct taccgg
//
LOCUS       DAOTKU010000092         4299 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_17545_length_4299_cov_3.329642, whole genome shotgun sequence.
ACCESSION   DAOTKU010000092 DAOTKU010000000
VERSION     DAOTKU010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4299
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17545_length_4299_cov_3.329642"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..716
                     /locus_tag="P5310_03205"
     CDS             <1..716
                     /locus_tag="P5310_03205"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002850542.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HRU32404.1"
                     /translation="HAKAYREISLLLKRPPGREAYPGDIFYLHSRLLERSAKLNDYLG
                     GGSLTALPIVETQQGDISAYIPTNLISITDGQIYLDSSLFYEGVRPAVDIGLSVSRVG
                     GSAQIKAMRQVSGRLRLDLAQYREVETFAQISTEIDSSTRAQLERGSRIREILKQPQY
                     SPMDVIDQVIILFTVTRGYIDEIALEEILHFQNNLLEYINKNYKELKEKIRTEGLTEE
                     NIKELTRAIFEFKRTFSSE"
     gene            716..1579
                     /locus_tag="P5310_03210"
     CDS             716..1579
                     /locus_tag="P5310_03210"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0042777 - proton motive force-driven plasma
                     membrane ATP synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FoF1 ATP synthase subunit gamma"
                     /protein_id="HRU32405.1"
                     /translation="MLSRQRLRRQISNVSHITDIIYSTELTSASRLRKSQSRLRSITP
                     YSESIERLLQDFAGNIDRENVKGFTLLESFNTDRELLLVIGGERGLCGGYIIDIVRKC
                     EDSIKSNTDIIVYGTRTGKTLYKRRFNVIEVNALNPLISFESIQLISKNIQDRYISGR
                     YGRVKAIYLSPKTAFTSIPAEVTLIPLEALKRGSRDIENYIIEPSSEEILRRLLPMGF
                     TMRLYRIFLEAITSEYSARRIAMHQAYENAKELLKNLNVTYQKLRQESITREIIEVST
                     SKVALEGERYE"
     gene            1563..2966
                     /gene="atpD"
                     /locus_tag="P5310_03215"
     CDS             1563..2966
                     /gene="atpD"
                     /locus_tag="P5310_03215"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015919341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HRU32406.1"
                     /translation="MKDMSKGYVLQVFGPVIDVQFQSIEDVPKVKDILILDKGTGEKL
                     SLETSIIMNNTIARCIALGPPEGIAKGMEVTNTKKSLHVPVGDKLLGRILNLFGEPID
                     SLGPIESEESWSIYTEAPPLERITPPNEIFETGIKAIDLLAPYPRGGKIGLFGGAGVG
                     KTVLIMELIHNVAVKHGGISIFAGVGERTREGNDLWLEMQRSGVIKNAVLIFGQMDEP
                     PGVRFRLPNTALTIAEYFREKKSTDVLLFIDNIFRFIQAGSEVSALLGRLPSAVGYQP
                     TLSTEVGELEERIVSTTKGAITSVQAVYIPADDPTDPAPATVFSHLDATIMLSREIAE
                     MGIYPAIDPLNSSSRLLNPYYVGERHYRIAREVQRIIEHYYRLRDIIAILGIEELSEE
                     EKEIVARARRIQRFLSQPMFTAEQFTGISGKYLTIEETLDSFEELLTGNLDTIPEQAF
                     FMMGNIDEVKEKAKKSQ"
     gene            2963..3370
                     /gene="atpC"
                     /locus_tag="P5310_03220"
     CDS             2963..3370
                     /gene="atpC"
                     /locus_tag="P5310_03220"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HRU32407.1"
                     /translation="MKLNVRVVTPDRLVYEGEADSVVIPGYDGYFGVLPNHAPMLSML
                     DMGELKIRDGVQEQYYAIDGGFCEISKNKVIIITPSAQRADEIDDNQVMATLRSARER
                     LHSKAEEKELLDLQITIRKASVLLEVSKHKLRR"
     gene            3375..>4299
                     /gene="murA"
                     /locus_tag="P5310_03225"
     CDS             3375..>4299
                     /gene="murA"
                     /locus_tag="P5310_03225"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HRU32408.1"
                     /translation="MRLRIEGGIKLKGEVDIIGAKNSALPIMAATLLTSGENIIYNVP
                     DLKDVHTMMNMLENFGAKLRFEDNTLYITIDEIQEVNPPEDQVKQMRASFLVAGPLLA
                     RLGKIKIPLPGGCQIGSRPVDLHLKGFQALGARVSSEHGYVELEGKNLKGTSIYLDIP
                     TVTGTENIIMAAVLAKGKTEIINPAKEPEVVDLADVLNKMGAKIKGAGSDRIEIEGVD
                     SLLPFNHTVIPDRIEAGTFLVAGAITGGEVTIRNVRKDHLGAVIGKLEEMGHRIETDE
                     DKIRIIAKNTGKACDITTAPYPGFPTDMQALF"
BASE COUNT         1451 a          725 c         1003 g         1120 t
ORIGIN      
        1 aacatgccaa ggcttataga gaaatatccc ttcttttgaa aagaccgcct ggaagagagg
       61 cttatcctgg agacatattc tatctgcact cgagactttt agaacgttct gcaaaattga
      121 atgattatct gggaggaggc tctctcactg ccttaccaat agtagaaaca caacagggag
      181 atatatcagc ctatatacct acaaacctca tctctataac tgacggacag atatatcttg
      241 attcatcgct cttttatgaa ggagtaagac cagcagttga tataggccta tctgtctcaa
      301 gggttggggg tagtgcacag ataaaggcta tgagacaggt ctccggaaga ctgaggttag
      361 acctcgctca gtatcgtgaa gtagagacat ttgcacagat atctactgag atagactcat
      421 ctaccagggc tcaactggag agaggaagtc gtataagaga aatattaaaa cagccacagt
      481 acagtccaat ggatgtaata gatcaggtta ttatcctgtt tacagttacc aggggataca
      541 tagatgagat agctttagaa gagatattgc acttccagaa taatctattg gaatatataa
      601 ataaaaacta taaagagcta aaagagaaaa taagaaccga aggattaaca gaagagaata
      661 ttaaagagct gaccagggct atatttgaat ttaagagaac cttcagtagc gaataatgtt
      721 atccagacag agattaagaa gacagataag taatgtcagt catataacag atataatata
      781 ttcaacagag ttaacctcgg cttctcgatt aagaaaaagt cagtccaggt tgaggagtat
      841 taccccctat agcgaatcta tagaacgttt actccaggat ttcgcaggga atatcgatag
      901 agaaaatgtc aagggtttca cacttttgga atctttcaat actgataggg agcttctact
      961 ggttataggt ggagagagag gactctgtgg aggatacata atagatattg tgaggaaatg
     1021 tgaagattct attaaatcta atactgacat tatcgtgtat gggacgagaa ctgggaagac
     1081 tctttacaaa agaagattta atgttataga ggtaaatgca cttaatcctc tgataagctt
     1141 tgaatccatt caacttatat ctaagaatat tcaggataga tatataagtg gtagatatgg
     1201 cagggtaaaa gctatatatc tttcgcctaa gacggctttt acctctatcc cggcggaagt
     1261 tactctgata ccattagaag cattaaaaag aggatcaagg gatatagaga actatataat
     1321 agagccttcc tcagaggaga tattaagaag attattgcca atggggttta ctatgagatt
     1381 atacaggata ttcttggaag ctattacaag tgaatattct gctaggagaa ttgctatgca
     1441 tcaggcatat gaaaatgcga aggaattatt aaaaaatctt aatgttacat accagaagct
     1501 cagacaggaa agtataacca gagagataat agaagtttct accagcaagg tagctcttga
     1561 gggtgaaaga tatgagtaaa ggatatgtcc tgcaggtatt tggacctgta atagatgtac
     1621 agtttcagtc aatagaagat gtcccaaagg ttaaagatat actcatcctg gataagggaa
     1681 caggagagaa attgtccctt gagacatcca taataatgaa taacactatt gctagatgta
     1741 tagcacttgg accacctgaa gggatagcca agggtatgga ggtaactaat actaaaaaat
     1801 cccttcacgt acctgtaggt gataaactct tgggaaggat tttaaatctc tttggtgagc
     1861 ccatcgatag tcttggaccc atagaatcgg aggaatcctg gtctatctat acagaggctc
     1921 cccctttgga aaggataact cctccaaatg agatatttga gacaggaatt aaagctatag
     1981 acctgttagc tccctatccc agaggtggaa agataggatt gtttggaggt gccggggtag
     2041 ggaagactgt gctgattatg gaacttatcc ataacgtagc ggtaaaacat ggtgggatat
     2101 ccatttttgc tggagtgggt gaaaggacca gagagggaaa tgatctctgg ctagaaatgc
     2161 agagatcagg ggtaataaaa aatgctgtac ttatctttgg tcagatggat gagccacccg
     2221 gagttagatt cagacttccc aatacagctc ttacaatagc agaatatttc agagagaaga
     2281 aatccaccga tgttctcctc tttatagata atatcttcag gttcatacag gcaggtagtg
     2341 aggtatccgc tcttttaggc agactccctt cagctgtagg atatcagccc actctctcta
     2401 ctgaggtagg agagttagag gaaagaattg tatctacaac taaaggtgca ataacttctg
     2461 ttcaggcagt ttatatacca gcagatgacc ctacagatcc ggctccagca actgttttta
     2521 gccatctgga cgccactatt atgctctcca gggagattgc tgaaatgggg atatatcctg
     2581 ccatagaccc actgaattct tcttcaagat tattaaatcc atactatgtg ggagaaagac
     2641 attacaggat agccagagaa gttcagcgga taatagaaca ttactacaga ttgagagata
     2701 taatagcaat actagggata gaagaactct ctgaagaaga gaaggagata gtagccaggg
     2761 caagacgtat acagaggttt ctatcacaac caatgtttac cgccgagcag tttaccggaa
     2821 tttcgggtaa atatctcacc attgaagaga ccctggatag ttttgaagaa ttgttaactg
     2881 gtaatctgga tactattcca gaacaggcat tctttatgat gggtaacatt gatgaggtta
     2941 aagaaaaggc taaaaagtca caatgaaact gaacgttaga gttgttactc cagacagatt
     3001 agtatacgaa ggtgaagctg atagtgtggt cattccagga tacgatgggt attttggtgt
     3061 cctgcctaac catgcaccta tgctctccat gctggatatg ggtgagttaa aaataagaga
     3121 tggggttcag gagcagtact atgctataga tggtggtttc tgtgagatca gtaagaataa
     3181 agttattatt atcactccgt ctgctcagag ggcagatgag atagatgata atcaggttat
     3241 ggcaaccctc aggtcagcca gagagagatt gcattcaaaa gcagaggaga aagaattgct
     3301 ggatttgcag attactataa gaaaggcttc tgttttactt gaagtctcta aacataagct
     3361 aaggagataa aaagatgaga ttgaggatag aaggtggaat aaagttaaaa ggagaagtgg
     3421 acataatagg agctaaaaat tctgccttac caataatggc agcaacccta cttaccagtg
     3481 gagaaaatat tatttacaat gtcccggatc tgaaagatgt ccatacaatg atgaacatgc
     3541 tggagaactt tggtgctaaa ctgaggttcg aagataatac cctgtatata actatagatg
     3601 agatacagga agtgaaccct ccagaagatc aggtaaaaca gatgagagct tcctttcttg
     3661 tagcaggacc cttgcttgct agattaggaa agataaaaat tcctcttcca ggaggatgtc
     3721 agatagggtc cagaccggta gacctgcatc taaagggatt tcaggctctt ggtgccagag
     3781 tctccagtga gcatggttat gtagagcttg aggggaagaa cttaaaggga acttccatat
     3841 acctcgatat tccaaccgtt actggaactg aaaatattat tatggcggct gtattagcca
     3901 agggaaaaac agagataatt aatccggcta aagaacccga agtggtggat ctggcagatg
     3961 tattaaataa gatgggagcg aagataaagg gtgctgggag tgacaggata gaaatagaag
     4021 gagtagactc cctcttaccc tttaatcaca ctgtaatccc tgataggata gaagctggaa
     4081 cttttctggt tgcgggagct atcaccggag gagaagttac catcagaaat gtcagaaaag
     4141 accaccttgg agctgtgata ggcaaactgg aagaaatggg gcacaggata gaaactgatg
     4201 aagataagat aagaattata gctaaaaata caggtaaggc atgtgatatt acaactgctc
     4261 cctacccagg attccctacc gatatgcagg cactttttg
//
LOCUS       DAOTKU010000093         4299 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_17546_length_4299_cov_3.067625, whole genome shotgun sequence.
ACCESSION   DAOTKU010000093 DAOTKU010000000
VERSION     DAOTKU010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4299
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17546_length_4299_cov_3.067625"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /locus_tag="P5310_03230"
     CDS             <1..727
                     /locus_tag="P5310_03230"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HRU32409.1"
                     /translation="ISFVDDTRREATLELMHCDEYVDLLIPRGGANLIQTVRRESTVP
                     VIETGVGNCHVYVDDSADLSMAEEITFNAKVQRPGVCNAIETLLVNEKVSREFLPEML
                     ERLRSAEVEVRGCQKTREIVPWVLPATEEDWYTEYLDLIIAVKVVEDVDSAIAHINKY
                     GSHHSESIVTRDYFNARKFLNEVDAACVYVNASTRFTDGFEFGLGAEIGISTQKLHAR
                     GPMGLEEITTTKYIIYGEGQIRG"
     gene            724..1509
                     /gene="proC"
                     /locus_tag="P5310_03235"
     CDS             724..1509
                     /gene="proC"
                     /locus_tag="P5310_03235"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HRU32410.1"
                     /translation="MSYNTGLIGLGNMATALIEGWIKKGRNPKEIIGFDIKRERCEFL
                     KRRFDIEIADRIEEISAKMVIIVIKPQDFSTLNLDLPVDTLIVSIMAGVPIRKIEEVF
                     DKNPVIRVMPNLLCSINKSFIPFACNEKVSKSDREFFIPIFRDFGEVVEIKESLMNIM
                     TALNGSGPGFVAYLIEAFTEAGVYNGLAYDTALKLTLSIFEGTASLLRENDISPNSFR
                     IDVTSPGGTTSRGVWVLEKSGLKGAIYDMLRVSGERAKELENS"
     gene            1533..2531
                     /locus_tag="P5310_03240"
     CDS             1533..2531
                     /locus_tag="P5310_03240"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRU32411.1"
                     /translation="MLRVGIIGAGVMGRTHAECWSRLEDVKIQVVCDVQVERAEALSR
                     TYNCDVSTSADDVIKRSDIDIVDICTPTPFHSEIAVSALEKGKHTLCEKPVARNLESA
                     RKVVKTAREVDVKFMTAHVVRFFPEYVTARNLIDQGAIGIPRMARTKRGGQYPLGRWN
                     DWFSDVDMSGGCLVDTGIHDFDYLRWIFGDVERVYASALTWRRLEHKDYGLVTLRFKS
                     KAMAQVEIIWAQPPGTPFNTGFELIGTDGLIAYNSQDSSSLRIMVSNGDNIVQRAESP
                     LTESPYLTEIRHFVSCIEENKEPSVTPEDAYKALEVAIASLESARINRPVSVGGDI"
     gene            2531..3520
                     /locus_tag="P5310_03245"
     CDS             2531..3520
                     /locus_tag="P5310_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016483099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRU32412.1"
                     /translation="MKGAILSFAHMHAYSYIECLKKISDVEISAIADEDTDRGKSLAD
                     RYGTRFYKDYQDLLKEEDVDFVVVCSENVKHRDMVIASAQAGKHILCEKPISVDTPSA
                     KEMIDAVNRYGVQLQIAFPVRFSPSVRRVKDMIDRKELGNILAFNCTNHGKMPGGWFV
                     EKSLAGGGAIMDHTVHVMDILRWYLGSEAEYVYAEGDTLLHNIPAEDTGLLSIIFENG
                     IFATIDASWSRPKSFPIWGDVNIEMVTEKGVVEMEVFSQTLEFYNDKSMSASWIDWGA
                     NMDLLMIQNFISSIKNGKPVEVTGYDGLKAMEVALAAYRSLELKEPVKLPLEV"
     gene            3522..>4299
                     /gene="nagA"
                     /locus_tag="P5310_03250"
     CDS             3522..>4299
                     /gene="nagA"
                     /locus_tag="P5310_03250"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HRU32413.1"
                     /translation="MFGLRGNIVANGNILNNWILVIEDDHIYDVIRETDKIPVENLSD
                     CYIMPGFVDIHTHGAVGYDVMDADPEGIVKMAEFLATQGVTSFLPTTTTLDIDTTLRA
                     ISSVETAKNLNKDGARILGIHLEGPFINPMHKGAQNEKFIIEPTKSVLDQLTSSGIIK
                     LITIAPEIPGAIEAIRLLRENGIYVSLGHSDATYEDTVKAIMAGASQVTHLFNGMRPF
                     HHREPGIIGAGLAEEALRCQIIADGIHLHFSSIRLVYKAKG"
BASE COUNT         1320 a          695 c         1093 g         1191 t
ORIGIN      
        1 gatatctttc gtcgatgata ccagacgaga ggcaaccctg gaacttatgc attgtgatga
       61 gtatgttgac cttttaatcc ccaggggagg ggcaaatctc attcagacag tgagaagaga
      121 gtctaccgta ccggttatag aaacaggggt gggaaattgt cacgtatatg tagatgatag
      181 tgccgatcta tctatggcag aagaaattac ctttaatgct aaggtccaga gaccaggtgt
      241 ttgtaatgcg atagagaccc tcctggtaaa tgaaaaggta agtagagaat ttctccctga
      301 gatgttagaa agacttagat ctgctgaggt ggaggtcaga gggtgccaga agacgagaga
      361 gatagtacct tgggttctcc cggcaacaga agaagattgg tatactgagt atcttgacct
      421 tataatagcg gtaaaagtgg tggaagatgt ggattcggct atagcccata taaataagta
      481 tggaagtcat cactctgaga gtattgtaac gagagactat tttaatgcca gaaagtttct
      541 caacgaagtt gatgctgcct gtgtatatgt taatgcatct accaggttta ctgatggatt
      601 tgaattcggt ttaggggcgg agataggtat aagtactcag aagttacatg ccagaggtcc
      661 tatgggatta gaagagataa ctacaactaa atatataatc tacggagagg ggcagataag
      721 aggatgagct ataatacagg tcttataggt ctaggaaata tggcaactgc acttattgag
      781 gggtggatta aaaagggaag gaaccctaaa gagattattg gttttgatat aaaaagagag
      841 agatgcgaat tcctgaagag gagatttgat atagagattg ctgacaggat agaggagatc
      901 agtgctaaaa tggtcattat tgttatcaag ccacaggatt tcagcacact caatcttgat
      961 cttccggtag atactcttat cgtctccata atggctggag ttcctataag gaagatagag
     1021 gaagtatttg ataaaaatcc agttataagg gttatgccca atcttctatg cagtataaat
     1081 aagagtttta ttccatttgc ctgtaatgag aaggtcagta agtctgacag ggaatttttt
     1141 atccctatct tcagagattt tggagaggtc gtggagataa aagaatcact tatgaatatt
     1201 atgactgcac ttaacgggag tggtccaggt tttgttgcct atcttataga ggcttttaca
     1261 gaggcaggtg tctataatgg acttgcatat gacactgctt taaaacttac actgagtata
     1321 tttgaaggaa ccgcatcttt actcagagaa aatgatatat ctcccaactc attcaggatt
     1381 gatgtaactt ctcctggcgg aaccacatcc agaggggtct gggttctgga aaaatctgga
     1441 ttaaagggag ccatatatga tatgcttaga gtcagtggtg aaagagctaa agaattagaa
     1501 aattcctgag agatagaaag gaagggagga ttatgttaag agtaggaatt attggagctg
     1561 gagtgatggg cagaactcat gcagagtgct ggtccagatt agaggatgtt aagattcagg
     1621 ttgtgtgtga tgtccaggtt gagagagcag aagcactgtc cagaacgtat aactgtgatg
     1681 tttccacctc ggctgacgat gttataaaaa ggtctgatat agatatcgta gatatatgta
     1741 cacccacgcc attccattca gaaattgccg tatcagcatt agaaaagggt aagcatacat
     1801 tatgtgaaaa gcctgtagcc aggaacctgg aatcagccag aaaggttgtt aaaacagcaa
     1861 gagaggtgga tgtgaagttt atgaccgccc atgttgtgag attcttcccg gaatatgtta
     1921 cagccaggaa tctgatagac cagggagcaa taggtatccc cagaatggca cggacaaaac
     1981 gtggaggaca gtacccttta ggaaggtgga acgactggtt ttcagatgtt gatatgagcg
     2041 gaggttgtct ggtggatact ggcatacatg acttcgatta tttaagatgg atatttggag
     2101 atgttgaacg tgtatatgcc agtgctctta cctggagaag gttggaacat aaagattatg
     2161 gattggttac cctcaggttt aaatctaagg cgatggctca ggtagaaatt atctgggcac
     2221 agcccccagg aaccccattt aatactggtt ttgaactgat aggtaccgat ggactcatag
     2281 cgtataacag tcaggactct tcttctttaa gaataatggt aagtaatggg gacaacatag
     2341 tgcaaagggc tgagagccct ttaactgaaa gcccatacct gacagagata aggcattttg
     2401 tctcctgtat agaagagaat aaggaaccat cagtgacccc tgaagatgcc tataaggctc
     2461 tggaggtcgc catagccagt cttgagtcag ccagaatcaa tagaccggta tctgttggag
     2521 gtgatatata atgaagggtg ctatattaag ttttgcccat atgcatgcct acagttatat
     2581 agaatgttta aagaagattt ctgatgtgga gatctctgcg atagctgatg aggatacaga
     2641 taggggaaag tctctggcag atagatatgg gaccagattt tataaggatt atcaggactt
     2701 attaaaagag gaagatgtgg attttgttgt tgtgtgttct gagaatgtaa agcatagaga
     2761 tatggtgata gccagtgctc aggctggtaa gcatatacta tgtgaaaaac caatctcggt
     2821 ggatacaccc tctgctaaag agatgattga tgcagtgaac agatacggtg ttcaattaca
     2881 gatcgcattt cctgtaagat tctctccctc tgtaagaagg gtaaaagata tgatagacag
     2941 gaaagaattg gggaatatcc ttgcctttaa ttgcaccaat catgggaaaa tgccgggtgg
     3001 ctggtttgtt gaaaaatctc tggcaggtgg tggggctata atggaccata ctgttcatgt
     3061 aatggacata ttaagatggt atcttggatc tgaggcggaa tatgtgtatg cagaagggga
     3121 cacactgctc cacaatatac cagcagaaga taccggactc ttaagtatta tctttgaaaa
     3181 tggaatattt gccactatag atgcaagctg gtccagacca aagtctttcc ccatatgggg
     3241 agatgtaaat atagaaatgg taacagagaa aggtgttgtt gagatggagg ttttcagtca
     3301 gaccctggaa ttttataatg ataaatctat gtctgcttcc tggatagatt ggggggctaa
     3361 tatggacctg cttatgattc agaattttat ctcttctata aagaatggaa agccggtaga
     3421 ggttacaggt tatgacggtc tgaaggctat ggaggttgcg ttagctgcct atcgatcctt
     3481 ggaattaaaa gaaccggtaa aactccctct ggaggtttag gatgtttggt ctgagaggaa
     3541 atattgtagc taatggaaat attcttaata attggattct ggttatagag gatgatcata
     3601 tatatgatgt tataagagag actgataaga taccggtgga gaatcttagc gattgctata
     3661 ttatgcctgg cttcgtagat atccatacac atggagcggt cgggtatgat gttatggatg
     3721 ctgatccgga gggtatagta aagatggcgg aatttctcgc cacccagggg gttacttctt
     3781 ttttacccac aaccaccacc cttgatatag atactacttt gagggctatc tccagtgtgg
     3841 agacggctaa aaatttaaat aaggatggag ccaggatact gggcatacat ctggaaggac
     3901 catttataaa tcccatgcat aaaggggcgc agaatgaaaa gtttataata gaacccacca
     3961 aatcggtgct ggatcagctc acatcttcag gtataattaa acttatcacc atcgctcctg
     4021 agattcctgg cgctatcgaa gccataaggc tcttaagaga gaacggtata tatgtctctc
     4081 ttggacactc tgatgccacc tatgaggata ccgttaaggc gataatggct ggagcaagcc
     4141 aggttaccca tttatttaat gggatgagac cttttcatca tcgtgaacct ggtataattg
     4201 gggctggact agctgaagaa gccctgaggt gtcagataat agctgatgga atacatctcc
     4261 atttctcttc tataagactt gtatataagg ctaaaggtt
//
LOCUS       DAOTKU010000094         4285 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_17611_length_4285_cov_3.675414, whole genome shotgun sequence.
ACCESSION   DAOTKU010000094 DAOTKU010000000
VERSION     DAOTKU010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4285
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17611_length_4285_cov_3.675414"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(186..262)
                     /locus_tag="P5310_03255"
     tRNA            complement(186..262)
                     /locus_tag="P5310_03255"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(226..228),aa:Arg,seq:tcg)
     gene            complement(271..348)
                     /locus_tag="P5310_03260"
     tRNA            complement(271..348)
                     /locus_tag="P5310_03260"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(311..313),aa:His,seq:gtg)
     gene            complement(352..428)
                     /locus_tag="P5310_03265"
     tRNA            complement(352..428)
                     /locus_tag="P5310_03265"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(392..394),aa:Arg,seq:tct)
     gene            complement(432..509)
                     /locus_tag="P5310_03270"
     tRNA            complement(432..509)
                     /locus_tag="P5310_03270"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(472..474),aa:Pro,seq:tgg)
     gene            complement(675..1643)
                     /locus_tag="P5310_03275"
     CDS             complement(675..1643)
                     /locus_tag="P5310_03275"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HRU32414.1"
                     /translation="MEVETFKGPVKSIGINGHPSMWNGDLKLFEDSLAFFESTGFTHI
                     EIPPHGLDIIMGGKINRERLERIRGIVQRFNLNYTVHAPDFLNLMDTVNSELHRSVFD
                     ASLEFTAEIGGKLLVYHAGRVPAEYAEDEVLIEELKAKERELLVEFSDLASRSDILIG
                     VENPNVDSDIVFRKVYTYGAFIDKLVDQVSKIARPNVGITFDFGHARVASTHFGFDYF
                     SSIELASTYINSLHVYDSFGRPNNVDRELPYMFQLIYGLGDLHLPPGWGNLPLEKSFS
                     NLKISQAFMTLEIHPGYKDEYAEGLKTAKYLASLINNNHMGEVRRG"
     gene            1914..2324
                     /locus_tag="P5310_03280"
     CDS             1914..2324
                     /locus_tag="P5310_03280"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HRU32415.1"
                     /translation="MKFEKEEPEKNFKPVEEWFKQAEYDLETAKAMFSTDRFIYTVFM
                     CHLTIEKALKGLYTKKFKSNAPKTHDLIYLVKKIELELLPSYRDFLETLNDLSVPTRY
                     PDDLERLLNQYRREKTEEILLKTEELFLWIRGNL"
     gene            2303..2638
                     /locus_tag="P5310_03285"
     CDS             2303..2638
                     /locus_tag="P5310_03285"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HRU32416.1"
                     /translation="MDKRKFIESIKESLIELLKERGISVELIVVFGSYATGKERDDSD
                     IDIIVVSKDFRDKDIFEIVEQTKDIHGELVERFMKPFDIMYYSDLDWEEGHSLIINSA
                     KEEGEIIYL"
     gene            2871..3686
                     /locus_tag="P5310_03290"
     CDS             2871..3686
                     /locus_tag="P5310_03290"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003370065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase subunit"
                     /protein_id="HRU32417.1"
                     /translation="MIEIDLSNFSGIVTGGASGIGKAVVKRLLEAGAEVVSADVNEKT
                     GEELLREFSEYRDRLEFVKADVTDKKSIDYMVSFTKERFKKINYLVNNAGINIPRLLV
                     DPQGKEEVTEEIFDKITVVNQKGAVLCAQAVAREMLKDGIKGVIVNMASESGLEGSEG
                     QSIYAGTKAALYSYTRSWAKELGKYGIRVVGLAPGILEATGLRTLEYERALAYTRGIT
                     VEELRRSYEAVSIPLKRVGKLEEVANLVCFLISDLASYITGTVVNISGGKSRA"
     gene            complement(3960..>4285)
                     /locus_tag="P5310_03295"
     CDS             complement(3960..>4285)
                     /locus_tag="P5310_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32418.1"
                     /translation="VEVASVLSDICKGGLWQRRLKTVEDIPRYWDDIIQLYKAKPVAF
                     FKGGGDGDVDYKPLWRTLKTQGGLNERTCARYGIGERAIVFHKNNGVSNAGGFIQNLI
                     SHSTG"
BASE COUNT         1347 a          859 c          895 g         1184 t
ORIGIN      
        1 acccgcgacc ctcagcttgg gaagctgatg ctctaccaac tgagctattc ccgcatccta
       61 gtaataatta tatttataaa ttatatccct gtcaagggga gtgtcaatag attatttcct
      121 gaaatataaa taaagctaac tccacgcaaa aatttacaga atcacagagt aattgataaa
      181 aaacttggcg cgcctgagag ggctcgaacc cccaacaagc ggatccgaag tccgctgctc
      241 tatccagttg agctacaggc gcaacaaaaa tggggtgaat gaggggactc gaacccccaa
      301 catctggatc cacagtccag cgctctgacc aagttgagct acattcacca atggcgcgcc
      361 cagcaggact cgaacccgcg gcccacagat tagaagtctg ttgctctatc cacctgagct
      421 atgggcgcaa atggtcgggg cgaaaggatt tgaaccttca gcctcttggt cccaaaccaa
      481 gcgcgctagc caagttgcgc tacgccccgc ctaacaataa atagtataac agatttttta
      541 ataaaatcaa tagcacgtag gttttttcta taaattaatt cacgtcctga atgattttca
      601 ttcatatagt gaataatatt acaaataccg gcaaaagtca agagctgatc tgcaatccat
      661 atggttgagc tattttatcc tctccgaact tcacccatat gattgttatt tatcaatgaa
      721 gctaggtatt ttgcagtttt tagaccttca gcatattcat ccttatatcc tggatgtatc
      781 tcaagcgtca taaaggcttg agatatctta agattcgaaa agctcttctc cagaggaaga
      841 tttccccagc caggtggaag atgaaggtcg cccagtccat agataagctg gaacatatat
      901 ggtagctctc tatccacatt attgggtctt ccaaaggaat cgtagacatg cagactattt
      961 atatatgtag atgcgagttc tatagaagaa aaataatcaa aaccaaagtg agtggatgcc
     1021 actctggcat gtccaaaatc gaaagtgata cccacgttgg gtcttgctat cttagatacc
     1081 tgatcgacca gcttatctat gaatgcacca taggtgtata ccttcctaaa gacaatatca
     1141 gagtccacat tggggttctc aacccctata aggatatcgc ttctggaagc taaatcagaa
     1201 aattccacca ataactctct ctccttagcc ttcagctctt cgataaggac ttcatcctca
     1261 gcatactctg ccggaactct acctgcatga taaaccagga gttttcctcc aatttcagct
     1321 gtaaattcca gggatgcatc gaagactgac ctgtgtaatt cagaatttac agtatccata
     1381 agattaagaa aatcaggggc atggacagtg taattcagat taaacctctg aacaatcccc
     1441 ctgatcctct ccaatctctc tctattgatc tttcctccca taattatatc caacccatgg
     1501 ggaggaatct ctatatgagt gaaacctgta gactcaaaga atgccagcga atcctcaaat
     1561 agttttaaat ctccattcca cattgaggga tgcccgttta tccctatact cttaacagga
     1621 cctttaaaag tctcaacttc catattcaat ttcctcctct ctcaatactg ttaaaattgc
     1681 ccaacttaaa gataatagga tagaaatggg aaaaatacaa gccactgtca atagtcactt
     1741 atataaaagg tggctgggag agggaagaag agaatttccc gttacctgtc acgcgtgacg
     1801 tgtcacggct tttatgtcac ccatcacgcg ttacgcgtga cttgttaata taatattgtt
     1861 ttaggtctca tattctgata ttattaatac gagaaagggg tgcaggcatc tctatgaaat
     1921 tcgagaaaga agaacctgaa aagaatttta aaccagtaga agagtggttt aaacaggctg
     1981 agtatgactt agagacggct aaagcaatgt tcagcacaga tagattcatc tataccgtct
     2041 ttatgtgtca tttgacaatt gaaaaggcac ttaaaggtct ttatacgaaa aaatttaagt
     2101 caaatgcacc taaaacccac gatctaattt atttagttaa gaaaattgag ttagagttat
     2161 tgccgtcata tcgagatttc ttggaaactc taaatgactt gagtgttcct accagatatc
     2221 ctgatgatct ggaaaggctc ttaaatcaat atagacgaga aaaaacagag gaaatactgc
     2281 ttaagacaga ggagctattt ttatggataa gaggaaattt atagagagca ttaaagagtc
     2341 tttgatagag ctattaaagg agaggggtat tagtgtagaa ttaatagttg tttttggctc
     2401 ttatgctaca ggaaaagaga gagatgatag cgacattgat atcatagtag tttcaaaaga
     2461 cttcagggat aaagatatct tcgaaattgt cgaacagaca aaagatattc atggcgagtt
     2521 ggttgagaga tttatgaagc cctttgatat tatgtactat tcagatttag attgggaaga
     2581 agggcactca ttaataataa attcagcaaa agaagaggga gagattatat acctgtaagg
     2641 agaaattatc tgacaccctc ggttttttct tccccacctc ggggaagcag cctctatctt
     2701 ccttctctat ttcggggaag caatctcttt ttcttcctcc cccacttcgg gggaggatag
     2761 aggtgggggc gtcacgcgtc acatttttag aaagtattag aaaggcagat tattcttgtg
     2821 gtatactatc cctgtattaa gattaatacc ttacttcagg aggcgaattt atgatagaaa
     2881 tagatttaag taatttctct ggtatagtta ccggcggagc aagtggaata ggtaaagcgg
     2941 tggtaaaaag gttactcgaa gcaggagcag aggttgtgag tgcagatgta aatgagaaaa
     3001 caggagagga actgctcagg gaattttcag agtacagaga tagattagaa tttgttaaag
     3061 cagatgtgac ggataaaaaa agtatagatt atatggtcag ctttacaaag gaaaggttta
     3121 aaaagatcaa ctatcttgtc aataatgctg gcataaatat cccaaggctc ttggttgacc
     3181 cacagggaaa ggaggaggta accgaagaga tctttgataa gataactgtg gtaaaccaga
     3241 aaggtgcagt cctctgcgcc caggcggttg ccagagaaat gctaaaggat gggataaaag
     3301 gggtaattgt aaatatggct tcagaatctg gactggaagg ttcagagggt cagagtatat
     3361 atgcaggcac aaaggctgcc ctttactcat atacaagaag ctgggcaaaa gagcttggaa
     3421 agtatggaat aagagtggtg gggctcgctc ctggaatact ggaggcaact ggacttagga
     3481 cattagaata tgagagagcc cttgcttata caagaggtat cacagtagaa gaattgagaa
     3541 ggagctatga agctgtatct attcctctta aaagagttgg aaagttagaa gaggtggcaa
     3601 atctggtatg cttcttaatc tcagacctgg ctagctatat aaccggaact gtcgtgaata
     3661 tatcaggcgg aaagtcaagg gcgtgaaatg aagggaaaga ctcatatgcc tcatctgcat
     3721 atctatttcc ccagacacag ctttaagatt agccatctgt tatcgttctg tgatattgtc
     3781 atgggtaaat agttcttgag aaattgtagg ggcaattcat gaattgcccc tacgggatat
     3841 aaagatgatt gatccatatc cttcatacta ttctgaacat ctctaacttt tactaccatt
     3901 aagttgttat tcaactcgac aatagcaacc tcctggcttt ctattctgta tagcttatat
     3961 cacccagtag aatgactaat caagttttga atgaatcctc ctgcattgga gacaccatta
     4021 tttttgtgaa agacaattgc ccgctcccca atcccatacc gggcacaggt tctctcattc
     4081 aagcctcctt gagtttttaa ggttctccat aagggcttat agtcaacgtc gccatcacct
     4141 ccacccttga aaaatgcaac cggctttgcc ttatagagct ggataatgtc gtcccagtat
     4201 ctggggatat cttccaccgt cttcagtctc ctctgccaca gccctccctt acagatatca
     4261 gaaaggacac tggcaacctc aaccc
//
LOCUS       DAOTKU010000095         4273 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_17662_length_4273_cov_3.547890, whole genome shotgun sequence.
ACCESSION   DAOTKU010000095 DAOTKU010000000
VERSION     DAOTKU010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4273
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17662_length_4273_cov_3.547890"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="P5310_03300"
     CDS             <1..229
                     /locus_tag="P5310_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32419.1"
                     /translation="EELKEYLGTERIDLLDLLAVSPFSACHILKTGKLIYSRGKDIEN
                     NYEMKIIRECCDLDVLRKKQMELIKEEFYGV"
     gene            305..2863
                     /locus_tag="P5310_03305"
     CDS             305..2863
                     /locus_tag="P5310_03305"
                     /inference="COORDINATES: protein motif:HMM:NF016071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HRU32420.1"
                     /translation="MRKKVFVLVIVFSVLINVNLLVLAESSERRSSYFQSNGDLINGW
                     YWLRDTALGHYAEWIFEDIPPGSEELILDITALATDRPGGGRGFDAKFKLIYGFPGNA
                     NMGGVLETKVVTLPNISSPDDPSGYTCHGQVTVDRAFISGATTILFRIERDSVWDNHI
                     AFKKDSIVLFPAGMEEIEEEQLSEGNQLPETDTLEGAILIQPGIYIGSLGDKDQEGHR
                     DNNDYYQINLKKGQLITLRLMVPGNANFNLSLLDSNRNSRGSSITQGNVESLGYVADS
                     TGSWYIKVSRSSGEGEYQLLVDIQNQNDAASGQDAGSSYQEAIPISAGTFNGFLKAGD
                     NEDYYSIGVVKGQLITLQLAIPGDASFNISLLDSNRNSRGSSVVQLNTKTLDYVADST
                     GIWYVKASRSSGEGWYQLLVNIQNQNDAASGQDAGSSYQEAIPISAGTFNGFLKAGDN
                     EDYYSINLQKGQQISLKLTIPGNASFNISLLNPNRNSRGSSITQGNMKTLDYIADSTG
                     TWYIKITRSSGEGEYQLSINAPDIHITQKPKLELSISNEGRGLISNLPIFVNESERGS
                     EIDLDGDGILQEFEDQAMDFINPKFELDEDEDWLKHPEHHVVNFVRVFPYPDKENHKY
                     VIFTYCITWSRDYGRTHLLGHDGDVERVIMAYEVISEGGKNLQLRYVFTSAHGGENDH
                     SAVWDAWNRSCSTCGISLWPFDEEMCGQLVFENNILKLQISEDKHAIYPTKDCCEGVH
                     LVHVKGVVFVMEDCVGGGTWRFNCYNAGEPDCYLIDDLDNPSSWKGLSEGKRTSLANL
                     FPQEQVWSGNKSHPGKFCGGLTGEGSKSDLWEDSPDQIGNKLKSLPGSLTDKLR"
     gene            3007..3759
                     /locus_tag="P5310_03310"
     CDS             3007..3759
                     /locus_tag="P5310_03310"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HRU32421.1"
                     /translation="MRFKKIGSRKIYIEVIEQIKNLIDSGEIKPGDQLPSERDLAEYM
                     GISRVSVRQALAVLEAIDVVDIKHGEGTFVSINKDSINNLNLFLSNISKESDPIDIID
                     ARKIIEVEIAGVAAKQRDENDLLSMRNLLSDMQQKIEKGEQTSTVDLSFHLAIATATH
                     NSVLLSVMNQIASLMRQNLWSIAKGLSLMTPGRAEKYLKQHRVIYGYIEDQDFLNAKG
                     AMRDHLLSIEQDLLGESKSNSKEEFYEKRFKR"
     gene            3737..>4273
                     /locus_tag="P5310_03315"
     CDS             3737..>4273
                     /locus_tag="P5310_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009255260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HRU32422.1"
                     /translation="MKKDLKDKTLGLIHASSITISIVKPFIQEIIPEVQVMHLCDDTI
                     QRDNIAAGVGVIPKVNYFKFATYAHFLEEAKVDLIMLMCSTFNRAVEFARPMINVPML
                     QIDRPMMELAVNTGKKIGIIATLPTTVPSSERLLKLVAEEAKKDIEVKTVLCSTAFKE
                     LEKGNVERHNEILLEEIDK"
BASE COUNT         1332 a          715 c          985 g         1241 t
ORIGIN      
        1 agaggagctt aaggaatatc tgggtactga aagaatagat cttcttgatc ttttagcggt
       61 ttctcctttt tcagcctgtc atattctaaa aacaggtaag cttatatact ccagaggaaa
      121 agatattgag aataactatg agatgaagat tataagagag tgttgtgacc tggatgtgct
      181 caggaaaaaa cagatggaac tgattaaaga ggagttctat ggagtttgat gtaaatctaa
      241 taattattaa ggagatatca gtttatattt tttagaaaat agtaaataag aggagggatg
      301 tattgtgaga aaaaaagttt ttgtgttggt tatcgtcttt tccgtcctga ttaatgtaaa
      361 tttattggtg cttgcagaaa gtagtgaaag gcggtccagt tatttccagt ccaatggtga
      421 tttaataaat ggctggtact ggcttcgaga cactgctctt ggtcattatg ctgaatggat
      481 ttttgaagat attcctccag ggtctgaaga attgatttta gatataactg cactggctac
      541 tgatcgacca ggtggaggca gaggttttga tgcaaaattc aaattaattt atggattccc
      601 cgggaatgct aatatgggag gcgtgcttga gaccaaagta gttacccttc ccaacatatc
      661 atctccggac gacccctcag gttatacctg ccatgggcag gtaactgtgg atagagcatt
      721 tatttccggt gcaaccacta tcctttttcg tatagaacgg gattcagtct gggataatca
      781 tattgccttt aaaaaagata gtattgttct attcccagca ggtatggaag aaatagaaga
      841 ggagcaactt tcagaaggaa accaattacc tgagacggat acattagaag gggcaattct
      901 tatacagcca ggtatttata tcggcagtct tggagataaa gaccaggaag gtcatcggga
      961 taataatgat tactatcaga ttaaccttaa aaaagggcaa ttaataaccc tgcgacttat
     1021 ggtccctggg aatgctaatt ttaacctttc cctgttagat tccaatcgca attccagggg
     1081 gtcttccatt actcaaggaa atgtggagtc attaggttat gtggctgatt ctacaggttc
     1141 ctggtatatc aaggtctctc gttcttccgg agaaggagag tatcagctgt tggttgacat
     1201 tcagaaccag aatgatgcgg caagcggaca ggatgctggc agttcttacc aggaagctat
     1261 tcccatatcc gccggtactt ttaacggatt tttaaaagca ggagataatg aggattatta
     1321 tagcattgga gtagtaaaag gacagttaat aaccctgcaa ctcgccatcc cgggggatgc
     1381 cagttttaac atctctctgt tagattccaa tcgcaattct cgaggttctt ctgttgttca
     1441 gttgaataca aagacactcg actatgttgc cgattccacc ggaatctggt atgtcaaggc
     1501 atcccgttct tcgggtgaag gatggtatca gctactggtt aacatccaga accagaatga
     1561 tgcagcaagc ggacaggatg ctggcagttc ttaccaggaa gctattccca tatccgccgg
     1621 tacttttaac ggatttttaa aagcaggaga taatgaggat tattacagta ttaacttaca
     1681 gaagggtcag caaatcagtt tgaagctgac tattccggga aatgccagtt ttaacatctc
     1741 tctgttgaac cccaatcgta attcccgtgg ttcttccatt acccagggaa atatgaagac
     1801 cctggattat atagctgatt ctacaggtac ctggtatatt aaaattactc gttcttccgg
     1861 cgaaggagaa tatcagctga gtataaacgc tccggatatc cacatcactc agaaacccaa
     1921 actggaatta agcatatcga atgagggaag ggggcttatc agtaacctgc caatttttgt
     1981 aaatgagtcc gagaggggtt ctgaaattga tttagatggt gatggaatat tacaggaatt
     2041 cgaagatcaa gctatggatt ttataaatcc taaatttgaa cttgatgaag atgaagattg
     2101 gcttaagcat ccagagcatc acgtggtaaa ttttgtccga gtatttcctt atcctgataa
     2161 agaaaaccat aagtatgtaa tatttactta ttgcatcacc tggagtaggg attatggaag
     2221 gacacatctt cttggacatg atggagatgt agaaagggtt atcatggcgt atgaagtaat
     2281 ttcagaagga ggtaagaatt tacaattaag atatgttttt acctcagctc atgggggcga
     2341 aaatgaccat tcagcggtgt gggatgcctg gaatcgaagc tgttccacct gtggaatttc
     2401 tctctggcca tttgatgaag agatgtgtgg gcaattagtc tttgagaata atattctaaa
     2461 gttacaaata tctgaggata aacatgctat ctatcctacc aaggattgct gtgaaggtgt
     2521 acatcttgtt catgttaaag gggtagtgtt tgttatggaa gattgtgtcg gcggaggtac
     2581 ctggaggttt aattgttata atgctggaga acctgactgc tacttgattg acgatctgga
     2641 taatccttct tcctggaaag ggctttcaga aggaaagaga acatcactgg ctaatttatt
     2701 cccacaagag caggtctgga gtggtaataa aagtcatcct ggtaaatttt gtggaggttt
     2761 aactggtgaa ggttcaaaat cagatctttg ggaggattct cctgaccaaa ttggcaataa
     2821 acttaagagt ttacctggtt cacttaccga taaactacga tgaactattt tataccctta
     2881 ctgaatctga aatatgaaga aacatatttc cccttgttta acattacaat atttttctat
     2941 tgacaaattt tttattactg ttatactgtc tccagtggtt agtccactaa ccactggaag
     3001 ggagaaatga gatttaagaa gataggttct agaaagatat atatagaagt gattgagcag
     3061 ataaagaact tgatagattc tggtgagata aaacccggag atcagcttcc ttcagagcgt
     3121 gaccttgcag aatatatggg tataagcagg gtctcagtaa gacaggctct tgcagtatta
     3181 gaggcgatag atgttgtgga tataaaacat ggagagggaa ctttcgtctc tataaacaag
     3241 gacagtatca ataatttaaa tttattccta tctaatatca gtaaagagag tgacccaatt
     3301 gatataattg atgccagaaa gataatagag gttgagattg ctggggttgc tgcaaagcag
     3361 agagatgaaa atgacctcct ttctatgagg aatctactct ctgatatgca gcagaagata
     3421 gaaaaagggg agcagacctc tactgtagat ctgagctttc atctggctat agcaactgca
     3481 acacacaact cggtgttatt atctgttatg aaccagatag cctcacttat gaggcagaat
     3541 ctatggagta tagcaaaagg tctcagtctt atgactcctg gtagggctga gaaatatctg
     3601 aagcaacaca gagttatcta tggatacatt gaagaccagg atttcctgaa tgccaagggg
     3661 gcaatgaggg atcatctttt gagtatagag caagacctgc ttggggagag taaatcgaac
     3721 agtaaggagg aattttatga aaaaagattt aaaagataag acattaggat tgattcatgc
     3781 ctcatcaata acaatatcta tagtaaagcc atttatccag gaaattatcc cagaggtaca
     3841 ggtgatgcat ctctgtgatg atacaatcca gagggataac attgctgcag gggttggagt
     3901 catcccgaag gtcaattatt ttaagtttgc tacctatgcc cactttctag aagaggcaaa
     3961 ggtagatctt attatgctga tgtgttcgac ctttaatagg gctgttgaat ttgctcgtcc
     4021 tatgataaat gtccctatgc ttcagataga tagaccgatg atggaattag ccgttaatac
     4081 tgggaagaag ataggtatca tagctacact gccaacaact gttccatcat cggaaaggct
     4141 tctgaaactt gtggctgaag aagcgaaaaa agatatagag gtaaagaccg tcctctgttc
     4201 taccgctttc aaagagcttg aaaaaggtaa tgtagaaagg cataacgaga tactgctgga
     4261 agagatagat aaa
//
LOCUS       DAOTKU010000096         4263 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_17696_length_4263_cov_2.529468, whole genome shotgun sequence.
ACCESSION   DAOTKU010000096 DAOTKU010000000
VERSION     DAOTKU010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4263
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17696_length_4263_cov_2.529468"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="P5310_03320"
     CDS             <1..212
                     /locus_tag="P5310_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32423.1"
                     /translation="SSYFSLDVKIGDIEGMVVPRELIKEENNKSYILILNKDSIEKRI
                     VKILGGNRERVVLDNIKEGEILVRN"
     gene            250..660
                     /locus_tag="P5310_03325"
     CDS             250..660
                     /locus_tag="P5310_03325"
                     /inference="COORDINATES: protein motif:HMM:NF016363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HRU32424.1"
                     /translation="MGLIDKLKRFLGLVDEDEFEAIDESLDTSSKVFPITSRKKKEGE
                     PVTVFIYGLNNFSEVQRVVNEVKLSKIVLVNFESASKEEAVKALDFVSGAIYALEGRL
                     EKIGDYVFLAVPKGIDVEDSTLTQDTYPHNTSRI"
     gene            711..1961
                     /gene="hisS"
                     /locus_tag="P5310_03330"
     CDS             711..1961
                     /gene="hisS"
                     /locus_tag="P5310_03330"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HRU32425.1"
                     /translation="MKPKYTRLRGVRDILPEELIHWRDVELKAREIFRRYNYKEIETP
                     IIEPAEIFIRTVGEETDIVRKEMYIFKDRGDRLVALRPEGTAPVVRAFLENKLYTPGK
                     VSKFYYIGDMFRYDRPQAGRQREFHQIGIEALGEASYILDVEVISLLLSFLKELGLKN
                     ITLLLNSVGCPNCRTVYSSALREFFTSRVEMLCEDCKERLERSPLRILDCKLDTCRDI
                     AGDAPSIMDYLCEDCKEHFDKVKEALTSAGIPYILDNRLVRGLDYYTKTAFEVKAEGF
                     DFSIGGGGRYDGLVKELGGPDIPGIGFAIGMERLLEVLEGSGLLLSKMEVPDYFIAYE
                     PDLVRDAIIFAERLRSRGFTVEISYTSKALKSQLREADRSKSRKAVIIKNELWINNRV
                     ILKDMTTGEQQEVESIKLEVCKFE"
     gene            1954..3714
                     /gene="aspS"
                     /locus_tag="P5310_03335"
     CDS             1954..3714
                     /gene="aspS"
                     /locus_tag="P5310_03335"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HRU32426.1"
                     /translation="MNKEFKRTEYCGELNIDDVGRQVVLNGWVNRRRDHGGLIFVDLR
                     DRTGIVQLVFDPKHNNDSFELAQQLKNEWVIGIKGKINKRPEGSENLKIPTGEIEILV
                     DKLTVFSRSNILPFNVNEEEDVDEYTRLRYRFLDLRRPRLQKNIELRHRVSQIVRKYL
                     SDSGFWEVETPMLTRSTPEGARDYLVPSRVHKGKFYALPQSPQLFKQMLMVSGIDRYF
                     QIARCFRDEDLRADRQPEFTQIDLEASFIDEEDIFSIIEGLMYSIFKGALDIELTLPF
                     PRMSYREAYTRFGTDRPDIRYDTELYDVSDILNKISFPPFQDKEVIAIKVSGRGDITR
                     KGIDELTDSARALGAKGLAWLSLQDEPRGPFSKFLNGHILDRLKTITNSDKGDLLLFI
                     ADEPGIARVTMGRFRLEINRRFFQPNTQYAFLWIVDFPLFSFNKDENRIEAEHHPFTS
                     PKDEDIHLLETEPLKVRANAYDLVLNGVELGSGSIRNSDPEMQKRVFSILGIDETDAY
                     KKFGFLLEALSYGAPPHGGMALGLDRIVAIMAGEESIREVLAFPKTQNAFCPLTEAPS
                     EVDENQLKELGISIRKEEVL"
     gene            3718..>4263
                     /locus_tag="P5310_03340"
     CDS             3718..>4263
                     /locus_tag="P5310_03340"
                     /inference="COORDINATES: protein motif:HMM:TIGR01944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HRU32427.1"
                     /translation="MNYILLPSLLLGVIGVGVGIMLSLAHKAFFVETSELQQRIRAAL
                     PGANCGACGYAGCDGLAEAISKGIAPANSCPVGGENAAEAIASILGIEVSSVQETKLI
                     FLCSGDKDKVSRKFEYTGVEDCSARLILWNGEISCQYGCLGGGTCVKSCPFSALSMGE
                     NGLPVIDYLKCKTCGICVQVCP"
BASE COUNT         1334 a          665 c         1067 g         1197 t
ORIGIN      
        1 agtccagtta tttttctctg gatgttaaga ttggagatat agaaggtatg gttgttccca
       61 gagaattgat aaaagaagag aataacaaat cctatatcct tatactgaat aaggattcta
      121 tagagaagag gattgtgaag atactgggag ggaatagaga gagagtagtt ttggataata
      181 taaaggaagg ggagatactg gtaagaaatt gaatgttaaa tccgcagata aaggaggtaa
      241 gataacaaaa tggggttaat tgataaactc aagagatttt taggtcttgt agatgaagat
      301 gaatttgagg ctattgatga gtctttagat acttcaagta aggttttccc gataacctcc
      361 aggaagaaaa aggaggggga acctgtcact gtatttatat acggtctgaa taacttcagc
      421 gaagttcagc gtgttgtaaa tgaggtaaaa ctatccaaga tagtgcttgt taatttcgaa
      481 tctgcatcta aagaggaggc tgtaaaagct ctggattttg ttagtggagc tatatatgcc
      541 ttggagggaa ggcttgagaa gataggggac tatgtatttc ttgctgtccc caaaggtata
      601 gatgttgaag actcaactct aacccaggat acctatcctc ataatacttc aaggatatga
      661 tataattttc aggcaggacc ttttatcgat attggaggga tattaagcct atgaaaccta
      721 aatatacacg gctaaggggg gtaagagata tattacccga agaactgata cactggagag
      781 atgttgaact taaggctcga gagatattca gaagatataa ttataaggag attgaaacac
      841 ccataataga acctgccgaa atatttatcc ggactgttgg agaagaaact gatatagtaa
      901 ggaaagagat gtatatcttt aaagatcgtg gagatagatt ggtggcttta agacctgaag
      961 gtaccgctcc tgtcgtcagg gcttttctcg aaaataaact ttatacccct ggtaaggtgt
     1021 ccaagtttta ttatataggt gatatgttca gatacgatag accccaggct ggtaggcaga
     1081 gagaattcca ccagataggt atagaggcat tgggagaggc ttcttatatc ttagatgtgg
     1141 aggttatatc tctcctgctg tcatttttaa aagagctggg attaaaaaat ataactctct
     1201 tattgaatag tgtgggttgt cccaactgca gaacagtata tagcagtgcc ttaagagaat
     1261 tctttacctc cagagttgaa atgttatgcg aagactgtaa ggagagatta gagagaagtc
     1321 ctctcaggat tttagattgt aagcttgata cctgcagaga tatagcaggt gatgctccat
     1381 caataatgga ttatctctgt gaggattgta aggaacattt cgataaggta aaagaggcat
     1441 taacttctgc aggtattcca tatattcttg ataatagact tgttagaggg ttagactact
     1501 ataccaaaac tgcatttgag gtaaaggcag aaggatttga tttctctatt ggtggtggag
     1561 gaaggtatga tggtctggta aaggagttag gaggtccgga tatccctggt atcggttttg
     1621 ccatcggaat ggagagactt ttggaagtcc ttgaaggtag tgggctccta cttagtaaaa
     1681 tggaagtccc tgattatttc atagcatacg aacctgacct ggtaagagac gccattatct
     1741 ttgcagagag attaagaagt agaggattta cagtggagat cagttataca tctaaagcct
     1801 taaaatctca actcagagaa gcagatagga gtaagtccag aaaggcagta ataattaaga
     1861 atgagttatg gattaacaac agggttatac tgaaagatat gactaccgga gaacagcagg
     1921 aggtagaatc cattaaattg gaggtatgta agtttgaata aggaatttaa gaggacagaa
     1981 tactgtggag agttgaatat agatgatgtt ggtaggcagg tagttttaaa tggatgggtg
     2041 aatagacgta gagaccacgg tggacttatc tttgtagatc tgagagacag gacaggtata
     2101 gtacagcttg tttttgatcc taaacacaat aatgactcat tcgaactggc ccagcagtta
     2161 aagaatgaat gggttatagg cataaaaggg aagataaata agagacctga aggttcagaa
     2221 aatttaaaga ttcctaccgg tgaaatagag attctggtag ataaacttac cgtattctcc
     2281 aggtcgaata tcttaccatt caacgtgaat gaagaggaag atgttgatga gtataccaga
     2341 ttaaggtata gattcctgga tctgagaaga ccaagactgc agaaaaacat agagttaaga
     2401 cacagggtca gtcaaattgt gaggaaatat ctttctgatt ccgggttctg ggaagtggaa
     2461 acccctatgc ttaccagaag cacccccgaa ggtgccaggg attatttggt tcccagcaga
     2521 gtccataagg ggaaatttta tgcactgcca caatcacctc aacttttcaa gcagatgctg
     2581 atggtttcgg gtatagatag atacttccag atagcaaggt gttttagaga cgaggactta
     2641 agggctgata gacagcctga atttacccag atagatttgg aagcttcttt catagacgaa
     2701 gaagatatct tcagtataat tgaaggtctg atgtactcaa ttttcaaggg ggctctcgat
     2761 attgaactta ccctgccatt ccccaggatg agctaccggg aggcttatac aaggtttggt
     2821 acagatagac cagatatcag gtacgataca gagttatatg atgtttcaga tatactcaat
     2881 aagataagtt ttcctccatt ccaggataaa gaggttatag ctataaaggt atccggtaga
     2941 ggggatataa ccagaaaggg tatagatgaa cttaccgatt ctgccagagc ccttggtgca
     3001 aagggattag cctggctcag tctacaggat gagcccagag gaccatttag taagtttctc
     3061 aatggtcata tcctggatcg attaaagact atcaccaatt ctgataaagg agacctcctc
     3121 ttatttatag ccgatgagcc cggtatagcc agggtgacta tggggaggtt cagattagag
     3181 ataaatagga gattctttca gccaaatacc cagtatgcgt ttctctggat agttgatttc
     3241 cctcttttct cgttcaataa agatgagaac aggatagagg cagagcatca tccgtttacc
     3301 tctcctaaag atgaagatat ccacctttta gagacagaac cattgaaagt aagagctaat
     3361 gcgtatgatc tggtattaaa tggggtcgag ctgggcagtg gaagtataag aaatagtgat
     3421 ccagaaatgc agaaaagggt cttctctatt ttaggtatag atgagacaga tgcttataaa
     3481 aagtttggat ttcttctgga agcactctct tacggtgctc cacctcatgg agggatggct
     3541 ttgggtcttg acaggatagt agcaattatg gctggtgagg agagtataag agaggttctg
     3601 gcattcccta aaactcagaa tgccttttgc cctcttacag aggctccttc agaggttgac
     3661 gaaaaccagc ttaaagaact gggtatctcg ataagaaagg aggaggtctt atagggtatg
     3721 aattacattc ttttaccgtc cttattgctc ggtgttatag gggttggggt tggtattatg
     3781 ttatctttag cccataaggc attttttgta gagacatctg aacttcaaca gagaataaga
     3841 gctgctctgc ctggtgcaaa ttgcggcgcc tgtggatatg ctggttgcga cggactggca
     3901 gaggcaatat ctaaaggtat agcccctgct aatagctgtc cggttggagg agaaaacgct
     3961 gcagaagcga tagcctctat tcttgggata gaggtatcct ctgttcagga gaccaaactg
     4021 atatttttat gttctggaga taaagacaag gtctccagaa agtttgaata tacaggggtt
     4081 gaagattgtt ctgcccgtct gatactctgg aatggtgaga ttagctgcca gtatgggtgt
     4141 cttggtggtg gaacgtgtgt aaaaagttgt cctttctctg cattgagtat gggggaaaat
     4201 ggactcccag taatagatta tctgaagtgt aaaacctgtg gtatctgtgt tcaggtctgc
     4261 ccc
//
LOCUS       DAOTKU010000097         4189 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_18045_length_4189_cov_3.502419, whole genome shotgun sequence.
ACCESSION   DAOTKU010000097 DAOTKU010000000
VERSION     DAOTKU010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4189
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18045_length_4189_cov_3.502419"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="P5310_03345"
     CDS             <1..292
                     /locus_tag="P5310_03345"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRU32428.1"
                     /translation="GLGQMIAMKYGTIPVVRGVGGLKDTVKEGETGFVFFDYTYTALL
                     ESIDRALNLYWNDRDGFYNMRKRVMLEDHSWDSSAKKYMELYSKILDLKRGV"
     gene            296..1315
                     /gene="galT"
                     /locus_tag="P5310_03350"
     CDS             296..1315
                     /gene="galT"
                     /locus_tag="P5310_03350"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HRU32429.1"
                     /translation="MSELRKDPVTRRWVIIATERAKRPSDFVKPKPDSSAIPEYLDKC
                     PFCEGNENQTPPELMSYRHAGTQKDSKGWWIRVVPNKYSAVSLDTHLERRGIGMYDMS
                     TGYGSHEIIVESPYHNRTMANMSEREIEEVLWAYRDRILEQARNPEIKYAIIFKNYGP
                     EAGASLEHPHSQLIALPVVPKRVLEEYEGARDYFAYKERCPYCDIIRQDKSDGERVIY
                     ENELFIALAPFASPSPYEVDILPKQHASTFEKIGKVEIMELSKIFKVIFLAISTVLSD
                     PPYNFILHNIPFRGNGTEFFHWHFNIVPRITKLAGFELGSGFYINPVKPEDAARTLRE
                     EINSL"
     gene            1327..2946
                     /locus_tag="P5310_03355"
     CDS             1327..2946
                     /locus_tag="P5310_03355"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HRU32430.1"
                     /translation="MNSVLYVALIWHHHQPTYKHPESGKYMLPWTRMHAVKDYFYMAN
                     LLAYFPRVNYTVNITPVLISQLNDYIYYYASDLYLDVTNKPVEELTLEDKRLIIENSF
                     VLNPEYFIKPYPAFYSLYRKVNSLGMERAIEELTDQDFLDLQVWGNLVWFDPHFRRDD
                     PVVKRLISEGHFTERHKSLLVRKQLEVMSLTLLRYNELVDLGQAEIITSPFYHPILPL
                     LIDRDVARETGINSLPELKFSYPGDARLQLESGIDLYKSSFGSRPSGIWPSELAVSND
                     ALNIIKSSGINWVIADEDILAKTFGKNLRDNECRLINPELLYRPYLVNTSSGKITMVF
                     RDKLLSNLISFDYSRWNSQNAAIDLIRRLEEIRFSLPSDKKYLVVIALDGENCWSYYP
                     ENGWEFLVSLYERLSSTAGLKAVTVSNFMEIDNNYDVLNGISPGSWINMSFDTWIGQP
                     ATNKAWDYLTRARQDLEDYRDKIDEKSYESALRELLIAEGSDWFWWYNRYHNVKEDKL
                     FDELFRAHLREVYKRINKQVPNYLYNPIEKS"
     gene            2973..>4189
                     /locus_tag="P5310_03360"
     CDS             2973..>4189
                     /locus_tag="P5310_03360"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HRU32431.1"
                     /translation="MSRKFNVKWTTSLLVVLSLLFLLPAVTFSSEVDSLTILHTNDTH
                     AHLDGVARRATLVKQIKKEVGIDNVLLLDAGDVFSGTPYFTIYKGSADVRFMNYIGYD
                     AMTIGNHEFDLGPEVLANFIGKARFPVLCANFDFSKEEPLKDSVKSWVIIEKGNEKYG
                     VFGLTTPETAEISSPGKNIVIRDYMDVAESIVKEMESAGIDKIIALTHIGWDNDVQLA
                     REVEGIDIIVGGHSHTLPNPYPMVVADDSTPTVVVQANEYGNYLGRLDVIFSETGVIQ
                     SWKGSLIPVDNKVPEENLCKIKLEDYKKPVTRLMNTAVGESLTDLDGDRNRVRSQETN
                     LGNLIADAMLEKAKIVNATIAIQNGGGIRASIPKGKITLGQIMTVLPFGNYLVTVDVT
                     GQQIIEALENGVS"
BASE COUNT         1303 a          695 c          990 g         1201 t
ORIGIN      
        1 cggacttgga cagatgatag cgatgaagta tggaactatc cctgtagtca ggggggtcgg
       61 aggattgaaa gatacggtga aagaagggga aaccggtttt gtcttcttcg attatacata
      121 taccgctctg ctagagagta ttgacagagc tctcaattta tactggaatg atagagatgg
      181 attctataat atgaggaaga gggtgatgct tgaagatcac tcctgggaca gttctgcgaa
      241 aaaatatatg gaactttatt cgaagatttt agatctgaaa agaggtgtat aatctgtgtc
      301 cgaactacgg aaagaccctg taactagaag atgggtgatt atagcaacag agagggctaa
      361 gagacccagc gattttgtaa aacctaagcc tgattcttcg gctatccctg aatatctgga
      421 taaatgtcct ttctgtgaag ggaacgagaa ccagaccccc ccagagctta tgtcctacag
      481 acatgctggt actcaaaaag attctaaggg ttggtggata agagttgtac ctaataaata
      541 ttccgcggta agtcttgata cacatttaga gagaaggggt atcggtatgt acgatatgtc
      601 caccggttat gggtctcacg agataatagt agagagtcca taccataacc gcacgatggc
      661 aaatatgtct gagagagaga tagaggaggt tctatgggca tacagggata gaattctgga
      721 gcaggcaagg aacccggaga ttaaatacgc tataatattt aaaaactatg gtccagaggc
      781 aggtgcttct ctggaacatc ctcactcaca actcatagcg ctgccagtgg ttcctaagag
      841 agtgctagaa gaatatgaag gtgccagaga ttatttcgca tataaagaaa ggtgcccata
      901 ctgtgatatt ataaggcagg ataagagtga tggagaaagg gtgatatatg agaatgaact
      961 ttttatagct cttgcacctt ttgcctcgcc atctccatac gaggtggata tcctgccaaa
     1021 acaacatgcc tctacgtttg aaaagattgg taaggtggag attatggaat tgtccaagat
     1081 atttaaggtt atatttctgg ctatttcgac tgtcctcagt gaccctccat acaatttcat
     1141 actgcataac atacccttta gaggtaatgg tacagagttc tttcactggc atttcaatat
     1201 tgtcccaagg attacaaagt tagccggttt tgagttaggc tctggatttt atataaatcc
     1261 ggtgaaacct gaagatgctg ccaggactct aagggaagag ataaattctc tataaggaga
     1321 gatataatga attcagtgct ttatgtagct ttgatctggc accaccatca acccacctat
     1381 aaacatccgg agagtgggaa atatatgctt ccctggacga ggatgcatgc agtgaaagat
     1441 tatttctata tggctaatct ccttgcctat ttccccaggg ttaactatac tgtaaacata
     1501 actccagttt tgatatccca attaaacgat tacatatact attatgccag tgacctttat
     1561 ctggatgtaa ctaataagcc agtagaagag ttgaccctgg aggataaaag acttataatt
     1621 gaaaattcct ttgtcttaaa ccccgaatat tttataaaac cctatccagc tttttactct
     1681 ttatatagga aggtaaattc tctcgggatg gagagggcta tagaggaact tacagatcaa
     1741 gattttttag accttcaggt ctgggggaac ctcgtctggt ttgatccaca ttttagaaga
     1801 gacgaccctg tagtaaagag attaataagt gaaggacatt ttactgaaag acataagagc
     1861 cttttagtaa ggaaacagct ggaagtaatg agtttgacat tactcagata taatgaactg
     1921 gtagatttag gtcaggcgga gattatcaca tctccctttt atcatccaat cttaccttta
     1981 ctcatagata gagatgtggc gagggaaact ggtataaata gtcttccaga attaaaattc
     2041 agctaccccg gggatgccag actgcagctt gaatcaggta tagatttgta taaatcttca
     2101 tttggatcaa gaccttctgg tatatggcca tctgaactgg cagtcagtaa tgatgcgttg
     2161 aacataataa aatcatctgg aattaactgg gtgatagcgg atgaggatat tctggcaaag
     2221 acttttggaa agaacttaag ggataatgag tgcagattaa tcaaccctga acttttatac
     2281 agaccttatc tggttaatac atccagtggt aagatcacta tggttttcag ggataaactt
     2341 ttatctaacc ttataagttt tgattattcc aggtggaact ctcaaaatgc agcgatagat
     2401 ctaatcagaa gattggagga gatcagattc tcccttcctt cagataagaa atatctggtg
     2461 gtgatagctc tggatgggga aaattgctgg agttattatc ctgagaacgg ctgggaattt
     2521 cttgtctctc tatatgagag attatccagt actgcaggat taaaagctgt aactgtctct
     2581 aactttatgg aaatagataa taactacgat gtattgaatg gtatctctcc cgggtcatgg
     2641 ataaatatgt cttttgatac atggataggg cagcctgcta caaataaggc ctgggactat
     2701 ttaaccagag ctagacaaga tcttgaagat tacagggata aaatcgatga aaagtcctac
     2761 gaatcagctc ttagagaact gttaattgca gaggggagtg attggttctg gtggtataat
     2821 agataccata atgtcaaaga ggataaactc tttgatgaac tttttagagc tcatctgaga
     2881 gaggtttata agaggattaa taaacaagtc cctaattatt tatacaatcc tatagagaaa
     2941 agttagattc ataatactgg aggtgaagaa ttatgagcag aaagtttaat gtcaagtgga
     3001 ctacctctct tttggtagtt ctgtctctcc tgttcctgtt accggcggtt acattcagtt
     3061 ctgaggtgga tagtcttact atactccata ccaatgatac tcatgcccat ctggatggtg
     3121 tggctcgtag agctacgctt gtaaagcaga tcaaaaaaga ggtgggtatt gataatgtac
     3181 ttttactgga tgctggagat gtcttctccg gaaccccgta tttcacaatc tataagggtt
     3241 ctgctgatgt aagatttatg aattatatag gttatgatgc tatgaccata gggaatcatg
     3301 agtttgatct gggacctgag gttctggcga attttattgg aaaggctaga ttccctgtat
     3361 tatgcgctaa ttttgatttt tctaaagagg aaccacttaa agatagtgta aagagttggg
     3421 tcattataga gaaagggaat gagaagtatg gtgtctttgg gttaaccact cctgagacag
     3481 ctgagatatc cagcccggga aagaatattg ttatcaggga ttatatggat gtggcagaga
     3541 gtatagtcaa agagatggaa tcggctggta tagataagat tattgctctt acacatatcg
     3601 gctgggataa tgatgttcag cttgccagag aagtggaagg gatagatatt attgttggtg
     3661 ggcattctca taccctgcca aatccttacc caatggtggt ggcagatgat agtacaccaa
     3721 ccgtggtggt tcaggctaat gagtatggaa attatcttgg acgtttggac gtgattttca
     3781 gcgaaaccgg ggtgatacag agctggaagg ggagtttaat accggttgat aacaaagttc
     3841 cagaagaaaa tctctgtaag atcaagctgg aagattataa aaaaccggtt accagactga
     3901 tgaatacagc cgttggtgaa tctctaactg acctggacgg agatagaaat agagtaaggt
     3961 ctcaggagac gaatcttgga aatcttatag cagatgctat gctggaaaag gcaaagattg
     4021 ttaatgcgac tattgcgata cagaacggtg ggggtataag ggcttccata cctaagggaa
     4081 agataacctt gggacagata atgacagtat tgccatttgg gaattatctg gttactgttg
     4141 atgttaccgg acaacagatt attgaagccc tagagaatgg ggtaagtca
//
LOCUS       DAOTKU010000098         4183 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_18083_length_4183_cov_4.233285, whole genome shotgun sequence.
ACCESSION   DAOTKU010000098 DAOTKU010000000
VERSION     DAOTKU010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18083_length_4183_cov_4.233285"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="P5310_03365"
     CDS             <1..367
                     /locus_tag="P5310_03365"
                     /inference="COORDINATES: protein motif:HMM:NF013012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HRU32432.1"
                     /translation="SGLKTAVIRLLKGKENIDLPSVAYSFQEAVVDVLVDKSLKACTN
                     YKINRLAISGGVAANVRLREKFLDEAKNYGIDVYFPSIKMCTDNATMICSATYYRYLA
                     GHRSDLTINAESYCGYPDG"
     gene            360..914
                     /locus_tag="P5310_03370"
     CDS             360..914
                     /locus_tag="P5310_03370"
                     /inference="COORDINATES: protein motif:HMM:NF025539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaC family protein"
                     /protein_id="HRU32433.1"
                     /translation="MDSLWEELEKRVRSYNLGELEREALLNIHQAREYLLALGDTSTL
                     TGPLYLFYYMVSLARVIYVCKKRSPFKEVLHGLTTRREETCVTVKANGTFPIFHSIIS
                     GKKIKPGTRFCIEELLGMIPWVSNVENTPLPAISASYLLGFLLSMLSRYEPVIWDRFR
                     EDHNINLFLKETPRMFLQEVIKNL"
     gene            complement(1010..1207)
                     /locus_tag="P5310_03375"
     CDS             complement(1010..1207)
                     /locus_tag="P5310_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32434.1"
                     /translation="MIDFAEGLFRTYQEGDEYIEYVEGIDSLRMERYQIWATSYIEWE
                     GLKYHLRVAKVIEEHLGGKYK"
     gene            1897..2883
                     /locus_tag="P5310_03380"
     CDS             1897..2883
                     /locus_tag="P5310_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRU32435.1"
                     /translation="MLNYIIRRIIYSIFLLFLVSIVGFVVIQLPPGDFLTVRIQELRN
                     RGDLSAEIYAEQLRARYGLDKPIWMQYIIWITNFVKGDFGWSFYYEKPVSELIGERLA
                     LTITISVITLIFTWALAIPIGVYSATHQYSWGDNIFTFLGFIGLSVPSFLLALLLMFI
                     SVFYFNQSVGGLFSPEYINAPWNFAKIIDLFRHLWIPVIIIGLSGTAGLIRIMRGNLL
                     DILGQQYILTARAKGLKESIVIWKHAVRVAINPLISSLGMSLPGIISGESLVSIVLNL
                     PTTGPLFLEALTRQDMFLAGSFLMFLTTALVAGNFIADLALAWADPRIRYEE"
     gene            2897..4027
                     /locus_tag="P5310_03385"
     CDS             2897..4027
                     /locus_tag="P5310_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRU32436.1"
                     /translation="MSKRERLEVSIEREPVRKSPTYWRLMWYKFKKNKRAVVGGIILI
                     LLYLICVVFTEFFAPYPLEYATDYISAPPQVLHFRDENGRFSIIPFVYGYKEEINYQL
                     FTREFKIDTSKKYRVKLFTKGYPYKLLGLFPADIHLFGTEEPDGVIFLFGTDRLGRDL
                     FSRVLYGGRISLTIGLLGVFLSLIFGSVLGTVSGYYGGTTDMIIQRTTELLMAFPNIP
                     LWMALATAVPPTWSPLAVYFAITIILSFLNWGGLARQIRGMVLSIRELDFVMAAKSFG
                     ADDRRIIFRHLIPNCMSHIIVISTLSIPGMILGETALSFLGLGLRPPMTSWGVLLYET
                     QNVRALRYTPWLIIPVFFVIITVLCYNFLGDGLRDAADPYSR"
     gene            4033..>4183
                     /locus_tag="P5310_03390"
     CDS             4033..>4183
                     /locus_tag="P5310_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009062748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRU32437.1"
                     /translation="MTENNLLLEVRDLRTYFPTYGGLVHAVDGVSFSMVKGKNLGVVG
                     ESGCGK"
BASE COUNT         1220 a          744 c          873 g         1346 t
ORIGIN      
        1 cagtggattg aagaccgctg tgataagact attgaaggga aaggagaaca ttgaccttcc
       61 atcggtagct tacagctttc aggaggcggt tgtggatgtg ctggtagata aatctctgaa
      121 agcctgcact aattataaga taaacaggct ggctatctct ggtggtgtcg ctgccaatgt
      181 aagactcaga gaaaaattcc tcgatgaagc taaaaattat ggcatagatg tctactttcc
      241 atccataaag atgtgtacag acaacgctac tatgatatgt tctgccactt actacaggta
      301 tctggcagga cacagaagcg accttaccat aaatgcagag agctactgtg gttatccaga
      361 tggatagcct gtgggaagag ctggagaaaa gggtcaggtc ttataatctg ggtgagcttg
      421 agcgagaggc tcttttaaat atacaccagg caagagagta tcttcttgcc cttggagata
      481 cttctacatt aacaggtccc ttatatctct tctattatat ggtaagtctg gcaagagtta
      541 tctatgtctg caaaaaaagg tcaccattta aagaggttct ccatgggctt accactagaa
      601 gagaggaaac ctgtgtcact gtaaaggcga atggaacctt ccccatattc cacagcatca
      661 tctctggcaa gaagatcaag ccagggacga ggttttgtat cgaagagctc ctgggtatga
      721 taccctgggt ctccaatgta gaaaatactc ctctccctgc tatttcagcc agttatctgc
      781 ttggtttttt gttgagtatg ttgtccagat atgaaccagt gatctgggat agattcagag
      841 aggaccataa tatcaatctc tttttaaaag agacccccag gatgtttctc caggaagtta
      901 ttaaaaactt gtaaatccct actcagagat accaccggct catagaagtt gagacaagtt
      961 tggggttaca aactcatcaa atacaggcat tcttctaaaa acgatgacat catttgtact
     1021 tacctcctaa atgctcctct attaccttcg ctaccctgag atgatatttt aatccttccc
     1081 attctatgta agatgttgcc catatctgat atctctccat ccttaacgag tctattcctt
     1141 ctacatattc tatgtattca tccccttcct ggtatgtcct gaataatccc tcagcaaagt
     1201 ctatcacttg tagcctctcc tcatctgtct ttactactcc atgacagcct agcttctcaa
     1261 tacaaaaact aaagaagttt ctattagcat atagtccatc agcaactgta tagtctacaa
     1321 atccctttcc atatgtcttt ctacaatacc caccttagct tttcctactt aaggtagttc
     1381 agctttaccc ttccctgaat taataacttt tagagcgata cttttaagta ttagccttag
     1441 agggtgtaga gacatgagat taagggttct agcgaaggta gaatcactgg caggctgaat
     1501 agatggtaga agtcttttac ttctaagtaa gaggtgaaag aagatagaga cacaagatat
     1561 cctgggtttc ttcctgttat ccgataccca tttggctagg aatcccaaga ggaagttctt
     1621 attacaaaaa tctataaaat gatgtatact tatttttagt ttttttggat gtgcatattt
     1681 aaattttaac tctcctatag aggataaata aagcctaagt ccctatagga gagttatttg
     1741 ttatagacta aaaagttttt gagtaatttc tgtgataatc catagtatct tgaagacctt
     1801 aactgattta tgatataatt ttttcagagt tgggtgtaat aggggtcagg aaaactataa
     1861 tgagatatta tagaatgctg gtaagaggtg ttatatatgc ttaactatat tattcgtaga
     1921 attatatatt ctatcttttt acttttctta gtttcaatag tgggttttgt tgttattcag
     1981 cttcccccag gagattttct aacagtaaga atacaggaat tgagaaatcg aggagacctt
     2041 agtgcagaga tctacgcaga gcagttaaga gctaggtatg gcctggataa accaatctgg
     2101 atgcagtata taatatggat aactaacttc gtaaagggtg attttggttg gtctttttac
     2161 tatgaaaaac cggtaagcga acttattggt gaaaggttag ctcttaccat aacaatatcc
     2221 gtgataaccc ttatttttac ttgggcatta gcaattccaa tcggtgtgta ttcagcaaca
     2281 catcaatatt cctggggaga taatatattc acatttttag gttttatagg attatctgtg
     2341 ccaagttttt tgttagcact cttattaatg ttcatatcgg tcttttactt taatcaatcc
     2401 gttggtggat tattttctcc agaatatata aatgcaccat ggaattttgc aaagataata
     2461 gacctctttc gacatctatg gattccggta ataattatag gtctttctgg aacggcaggt
     2521 ctgatccgta taatgagggg gaatctttta gatatcttgg gacaacagta tatccttacc
     2581 gctagggcta aagggcttaa agagtctata gtaatctgga agcatgcggt aagagtggca
     2641 ataaatcctc ttataagctc tttagggatg agtttacctg ggattatatc tggagaatca
     2701 ttggtatcaa tcgtactgaa tcttcctacc actggtcctc tattcttaga agctctcaca
     2761 agacaggata tgtttttggc tggaagtttc cttatgtttc tgactactgc ccttgtagca
     2821 ggtaatttca tagctgacct tgcattggct tgggctgacc ccaggataag atatgaggag
     2881 taatcggagg tagagcatgt ctaaaagaga aagattagag gtaagcatag agagagaacc
     2941 ggtaagaaaa agtcctacat actggagatt aatgtggtat aagttcaaga agaataaacg
     3001 ggctgtggtt gggggaatta ttctaattct tttatacctc atatgtgttg tttttactga
     3061 attctttgca ccataccctt tagagtatgc taccgattat ataagtgctc ctcctcaggt
     3121 cttgcatttt agagatgaaa atggcaggtt tagtattatt ccctttgttt atggatataa
     3181 agaagaaata aactatcaat tatttacaag agagttcaag atagatactt caaaaaagta
     3241 tcgtgtaaaa ctttttacca aagggtaccc ctataaactt ctcggtctat tccctgctga
     3301 tatacattta tttggtactg aagagcctga tggtgtaatc ttcctgtttg gaaccgatag
     3361 attgggaagg gacttatttt caagggtact ctatggtgga agaatatctc tgactatagg
     3421 attattaggc gtatttttaa gtctcatctt tggtagtgtt ttggggactg tttctggata
     3481 ctatggtgga accaccgata tgattattca gagaactaca gaacttctaa tggcatttcc
     3541 aaacatacca ttatggatgg cattagctac tgcagttcct cctacctggt caccattagc
     3601 agtatatttt gctataacta taattctttc attcctcaac tggggtggct tagccagaca
     3661 gataagaggg atggttttat ctatcagaga gctggacttt gttatggctg ctaaaagttt
     3721 tggggcagat gatagaagga taatctttcg tcatcttata cccaattgca tgagtcatat
     3781 catagtgata agtacactta gtattccagg gatgattctc ggcgaaaccg ctttgagttt
     3841 tctaggttta gggttacgtc ctccaatgac aagctggggt gtattactat atgagacaca
     3901 aaatgtaaga gctctcaggt acaccccctg gttaattata cctgtattct ttgtgataat
     3961 aactgttcta tgttacaact tcttggggga tgggttgaga gatgcagctg atccatattc
     4021 caggtgatat ctatgacaga gaataatcta ttactagaag ttagagattt aagaacgtat
     4081 ttcccaacat acggtggtct ggtacatgcg gtagacgggg tctctttcag tatggtaaag
     4141 ggcaaaaatc tgggggttgt tggtgagagt ggctgtggaa aga
//
LOCUS       DAOTKU010000099         4146 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_18279_length_4146_cov_3.272794, whole genome shotgun sequence.
ACCESSION   DAOTKU010000099 DAOTKU010000000
VERSION     DAOTKU010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4146
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18279_length_4146_cov_3.272794"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..901
                     /locus_tag="P5310_03395"
     CDS             <1..901
                     /locus_tag="P5310_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HRU32438.1"
                     /translation="ILSAIIIALIWEWKKDIPQHLWYVIMLYFLVYIIFVWYGLLRIK
                     ATERELDGHYVASKLLSKSLQLPIIVLSIVIISSIILPWGYSPLSLTSLGNFLARLFQ
                     PKPVDYGTPLPNSLFWRFRTSFLRPKMSLPPSGKSIYIPPYVLWILAGTGLIFVLLFL
                     IKKGFFSRVVSILKEGFTRLLYSVVEITKDLRSLGKINVSDVRMPRLPVREKPTTLMG
                     WIIYYYKVSLNLMAKKNLGKEVWETPYEYARRLGVLRPDIGDSYRELTEIFVTSRYKR
                     ITPKKDWISTMKNKIKEVKEKLG"
     gene            898..2316
                     /locus_tag="P5310_03400"
     CDS             898..2316
                     /locus_tag="P5310_03400"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HRU32439.1"
                     /translation="MREIFVGLDLGTQGVRTTAVDKNGNIVSSSVENFIDGTRDPELW
                     WDNCKKSFSSTVSRLNSQDRILSLVVTSTSGTLICVDDNGNPLADALMYNDPRGVEES
                     EILNKKLDWLTEKLGYKFNSSFGLSKVLWVKNNLPDIYEKTRYFLSPTDFINFKFTGE
                     LGITDHTNALKFGFDLIDYKWSEELEELGIDLLKLPGVVKPGEFLGNLRKELLIELGI
                     TYDIPLFTGLTDGTGGQIASGAVRVGESSTTLGTTLVIKGVTSMLVKDPLGRFYSHLH
                     PEGYGWFPGGSSNVGGEALEKIFPGEDYRELDSRASSIIPTSLIIYPLVREGERFPFV
                     EPRAKGFVAGEPSSRDELYAGYLEGIAYVERLCYDTLSKMSIDFGNMVYTVGGGSRSL
                     IWLKIRASVMNKVLKRPEIASSGMGAAILGASRTFYSSLNTAVSRMVRIELEVEPERY
                     LVSRYESNYKRFIEELSERGYL"
     gene            2313..3917
                     /locus_tag="P5310_03405"
     CDS             2313..3917
                     /locus_tag="P5310_03405"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRU32440.1"
                     /translation="MIDINILDGHSVIGGNKILVEGEDGAIMLDFGINFSSWGKYFEE
                     FLSPRSGLMVKDLLKLGLLPRLDIYRSDFAYDSDLPKVKKDILFTFLSHVHTDHMGFV
                     GLLKENIPILSSAETLAMAIAINEIHSEEKSRLILERRTPGEYPIRSDVLVKSRAKEK
                     RELVKRTLIYSGQNKISALPISVKDIEETFEEVNVEDSLAANLVEAKILPVYHSVIGS
                     SSLYFKLDGVRFLYTGDLRDSPSPDEERLLLEMGKNRLELANSTRKMIDLVKGKVDVL
                     IVEGTRVKGSHSNITEATLYGNILEEIEKSRGKLVIADFPLRHLERFHTFLKVANETG
                     REFVVLPKDYLLLSTMAVINSDWKFQRYVNCIRVYHQGKSSWNGWENKFLNGKIFNDP
                     PLEKLIIHPSEIEKDPGSYILNLGYFELSNLLDFSTSTLNGGIYIHSNSETYTEDQVI
                     DSNRLLNWLEYFNIEPKGIERGEDKNELSFAGNYHASGHISPEGLENLIEEVNPAIII
                     PVHSESPEWFSERWKEKVRLDHEVYI"
     gene            3921..>4146
                     /locus_tag="P5310_03410"
     CDS             3921..>4146
                     /locus_tag="P5310_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HRU32441.1"
                     /translation="MVKDFSFQWHITDLCNLRCRHCYQDRFDKERELPVEDCERIISD
                     MVTSLEVSGYESLSINITGGEPLISPLLYPI"
BASE COUNT         1271 a          707 c          961 g         1207 t
ORIGIN      
        1 cattttatcc gcaataataa ttgctttgat atgggaatgg aagaaggata ttccacagca
       61 tctttggtat gtcattatgc tttatttctt ggtgtatata atatttgtat ggtacggact
      121 tttaaggata aaagcaacag aaagggagct agatggtcac tatgtcgcca gtaagctcct
      181 aagtaaaagt cttcagcttc ccattatagt cctgagtata gtcattatat ccagtataat
      241 cctcccctgg ggatattctc cactctcttt aaccagtctg ggcaactttc ttgccagact
      301 cttccaacca aaacctgtag attacggaac acctttacct aactctcttt tctggaggtt
      361 cagaacctca tttctgagac caaaaatgtc tcttcctcct tctggaaagt cgatatatat
      421 accaccatat gtactctgga ttctggctgg tacaggttta atttttgtat tactctttct
      481 tataaaaaag ggctttttct ccagggttgt atctatatta aaagagggtt ttacaagatt
      541 gttatattct gtggtggaaa taacaaaaga tttaaggtct ctgggtaaga taaatgtatc
      601 tgatgtaaga atgcccaggt tacctgtgag agaaaaacca actactctga tggggtggat
      661 tatctactac tataaagtct ctctgaatct tatggctaag aagaatctgg gtaaagaggt
      721 atgggagact ccatatgagt atgccaggag gcttggagtt ttaagaccgg atatcggtga
      781 ttcttaccgt gaacttacgg agatatttgt tacctcaaga tataagcgga ttactccaaa
      841 aaaagactgg atatctacta tgaaaaacaa aataaaagag gtgaaagaaa aactgggatg
      901 agagagatat ttgttggatt agaccttgga actcaggggg tcagaactac cgcagtggat
      961 aaaaatggta atatagtttc ctcatctgta gagaatttta ttgatgggac aagggatcct
     1021 gagttatggt gggataattg taaaaagtcg ttctcttcta ctgtatccag gttaaatagt
     1081 caggacagga ttttatccct tgtcgttaca tctacctctg gcacactgat ctgtgtggac
     1141 gataatggga atcccctggc tgatgcttta atgtataatg acccgagagg ggttgaggaa
     1201 tctgagatct tgaataaaaa attagactgg cttaccgaaa aactcggata taagttcaat
     1261 tcctcttttg gactttccaa ggttctatgg gtgaaaaata atctccccga tatctatgag
     1321 aagacaaggt atttcctctc ccccaccgat tttataaact tcaaatttac cggagagctt
     1381 ggtattactg accataccaa tgcgctgaaa tttggttttg atctgataga ctacaaatgg
     1441 tcagaagagt tagaagaact tggcattgac ctgctcaaat tgcctggagt tgtgaaacct
     1501 ggtgaattcc tgggaaattt aaggaaagaa ctgctgattg agctggggat aacgtatgat
     1561 ataccccttt ttacaggact taccgatgga acaggtgggc agatagcttc gggggcggtt
     1621 agggttggtg aatcttccac cactcttggg accactctgg tgattaaagg ggtaacctct
     1681 atgttggtta aggaccctct cgggagattt tacagccatc tccatcccga aggatatggg
     1741 tggtttccag gaggttcaag taatgttgga ggagaggcgt tggagaagat cttccctgga
     1801 gaagattaca gagagctgga tagcagggct tccagtataa taccaacatc tcttatcatt
     1861 tatcccttag tcagagaggg ggagagattt ccatttgtag aaccaagggc taaaggtttt
     1921 gttgccggag aaccatcttc cagggatgaa ctctatgccg gatatctaga aggtatagcc
     1981 tatgttgaga gattgtgtta tgataccctt tctaagatga gtatagactt tggtaatatg
     2041 gtttacacgg taggaggagg ttccaggagt cttatatggc ttaagataag agccagcgtt
     2101 atgaataaag tcttaaagag acctgagata gcctcttctg gtatgggggc agcgatactt
     2161 ggggcgagca gaacattcta ttcctccctg aatacagctg tatccaggat ggtcaggata
     2221 gaactggagg tggagcctga gagatatctg gtttcacggt acgaatccaa ttataaaaga
     2281 tttatagaag aactctcaga gagaggatat ctatgataga tataaatatc cttgatggtc
     2341 acagtgtgat aggtggtaac aagatcttgg tggaaggaga agatggggct attatgctcg
     2401 attttggcat aaacttttct agctggggaa aatattttga agaatttctc tcccccagaa
     2461 gtggattgat ggtgaaggac ctcttgaaac ttggacttct tccacgactt gatatataca
     2521 ggagtgattt tgcatatgat tcagaccttc caaaggtaaa aaaagatatc ctgtttacat
     2581 tcctttccca tgttcataca gaccatatgg gctttgttgg gctcttaaaa gagaatatcc
     2641 ctatcctttc cagtgcagag acactggcta tggctattgc tataaatgag attcattccg
     2701 aagagaagag cagattgata ctggagagaa ggacgcctgg agaatacccc ataagaagtg
     2761 atgtactggt taaatccaga gctaaggaaa agagagaact tgtcaaaaga acacttatct
     2821 attcagggca gaataaaatc tctgcacttc caatatctgt aaaggatata gaggagactt
     2881 ttgaagaggt aaatgtagag gactccttag ctgcaaatct ggtggaggct aagattttac
     2941 ctgtatacca ttcagttatc ggttcttcca gtttatactt taagttagat ggggtaagat
     3001 ttttgtacac gggagacctg agggatagcc cttctcctga tgaggaaaga ctcttactgg
     3061 aaatgggtaa gaacaggctt gaactggcaa attccacaag aaagatgata gaccttgtaa
     3121 agggtaaggt ggatgtactt atagttgagg ggaccagggt gaaagggtct catagcaata
     3181 ttacagaagc caccctttat ggtaatattc ttgaagagat agaaaagagc agaggtaaac
     3241 ttgtaattgc agattttccc ctcagacacc tggagagatt ccataccttt ctcaaggtag
     3301 ccaatgaaac agggagagag tttgtcgttc ttcctaagga ttatctattg ctaagtacta
     3361 tggcggtcat aaattcagac tggaaatttc agagatatgt aaactgtata agggtttatc
     3421 accaggggaa gagcagctgg aacggctggg agaataaatt tttaaatgga aagatattta
     3481 acgatccacc gctcgaaaag cttattatac acccatcaga gatagaaaag gacccaggtt
     3541 cctacatatt aaacctcggt tactttgaac tatcaaatct cctggacttt tctaccagta
     3601 cattaaatgg aggtatctat atacattcta atagcgaaac gtatacagag gaccaggtta
     3661 tagactctaa caggctttta aactggttag aatatttcaa tatagaacca aagggtatag
     3721 agaggggaga ggataaaaat gaactctctt ttgctgggaa ttaccatgcc tccgggcata
     3781 tatcgcctga agggctggaa aatctcatag aagaggtgaa tccagctatt ataatccctg
     3841 ttcattcaga aagtcccgaa tggttttcag agagatggaa agaaaaagtg aggttagatc
     3901 atgaggtgta tatctaaaat atggtaaaag atttttcttt tcagtggcat attacagatc
     3961 tgtgcaattt aagatgcaga cattgttatc aggatcgatt tgataaagag agagagctac
     4021 ctgtagagga ttgtgagaga attatctctg atatggttac ctctttagag gtctctggat
     4081 atgaaagctt gagtataaat attactggag gagagccgtt aatatcacca cttctttatc
     4141 ccattc
//
LOCUS       DAOTKU010000100         4137 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_18331_length_4137_cov_3.407398, whole genome shotgun sequence.
ACCESSION   DAOTKU010000100 DAOTKU010000000
VERSION     DAOTKU010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4137
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18331_length_4137_cov_3.407398"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="P5310_03415"
     CDS             <1..666
                     /locus_tag="P5310_03415"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA prenyltransferase family protein"
                     /protein_id="HRU32442.1"
                     /translation="PLKSKRPLASGRLKVSYARLAVAILLPLSLIGSYLLHRRFFMVV
                     AGYILLQILYTLYIKDIPILDVLSISFGSLLRVVAGTVVIDVSISSWLIICTILLALL
                     LALSKRRYELINLEDAGVKRKVLKDYDLQLLDQMINITTSSVLIAYTLYTMSQRTIER
                     FDTSDLLFTVPFVLYGLFRYLYLIYRRGFGESPEEAFIIDKPLMIDIILWIIIVFVII
                     YIL"
     gene            680..1756
                     /locus_tag="P5310_03420"
     CDS             680..1756
                     /locus_tag="P5310_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013194683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HRU32443.1"
                     /translation="MARSTVVVLKTGPKGVLDDYGRLMRLSRYGDFVRRDIDIILKLN
                     LSWTRYFPACSSTPWQVEGVIKTLLDDGYERERIFPVENRTVVTNPIKGAINNKWLPV
                     LERYGLHFIPLTTVRWIKYETRTKLLRLNEIFPEGFEIPEMFIGKNIIHLPTVKTHGH
                     SITTGSIKNAFGGLLKEVRHYCHKYIHEVLVDLMIIQREIHPAIFTVMDGTVCGDGAG
                     PRTMIPRIENCILAGFDPVAIDAVSAKMMGFNPLDIPYIGMCNDMKLGVGDLSNIDII
                     GEDISEVNFGFKTKRSFVIWGDQMVRKGPLRFLEKPLLYSPLMVLPTLASNIYHDYIW
                     YPVIGRSRIKDFMKTEWGELFKRY"
     gene            1774..3558
                     /locus_tag="P5310_03425"
     CDS             1774..3558
                     /locus_tag="P5310_03425"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRU32444.1"
                     /translation="MDYILKERKLFLFALIISILFLYGSINISYNMYDEGIVVYGASR
                     ILKGDMPYRDFWTMYAPGQFYTVAFLFKIFGENLFVTRVYSATINLLIVLLVYFIVRK
                     VSDERLALLSLVLSTLWMGGWGLFYSSPTPAGTFWSLLGIYFVIDFLSTEKLSSLFTG
                     GIITGIGAIFRHDIGGYTFIASTIVILFYAYLKISDRSIKDTIKLWGRYLAGTVAGFL
                     PFAIYFLVNVPFGEIIYDLIIFPGKVFLRVRDLPYPGFGFNLYSFLFYFPIIIYLISG
                     IFIVLNIFKLKYFKGNEWVNLLFLILGLFFFNQAWVRSDVPHLLPTIIPAIMLLSSLY
                     KSELIKILGRREIIIKILLLLMGFILTLALYLNALKGLVIAVYALPLVLGTVISIIIY
                     IPVQFRRFYRDIPAKATLQVVSLVLSVFVIFSCFRDFKGVSIQRIFSFKIPGLVTSQF
                     ERAKGIYVFQGQETYLEPAIKIVRANTKPDELIFVGNSRHDRIFINDIMFYFLCERHS
                     ATKYHELHPGLANTRKVQLEIIDELKRNNVKIIVLWNGAENVVEPNESSVSTGVNDLD
                     DFIRLNYATIMYLGPYRILQRITGGEIG"
     gene            3555..3899
                     /locus_tag="P5310_03430"
     CDS             3555..3899
                     /locus_tag="P5310_03430"
                     /inference="COORDINATES: protein motif:HMM:NF013089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="HRU32445.1"
                     /translation="MIYILLVLSLISNALANVFIKAGMRDYTGGILHIFHHLLRSPLV
                     LAGLLFFGLAFIGYSFVLSRLELSIAYPIMTGAGFAIVSIFSFILFGESFSPVKIIGI
                     IFVFIGIILLSR"
     gene            4035..>4137
                     /locus_tag="P5310_03435"
     CDS             4035..>4137
                     /locus_tag="P5310_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013913965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HRU32446.1"
                     /translation="MKLGVFAVLFQDLPVEEALDKIVSYGLSAVEVGC"
BASE COUNT         1146 a          662 c          900 g         1429 t
ORIGIN      
        1 cctttaaagt ctaaaagacc acttgcttcc ggtagactta aggtttctta tgccaggctt
       61 gcagtggcta tcttgctgcc attatctctg ataggctctt atcttttgca tcgtagattt
      121 ttcatggttg tggcaggcta tatattactt cagattttat ataccctcta tatcaaggat
      181 attccgatat tagatgtact ttccatctca tttggctcac tcctcagggt ggttgcagga
      241 acagtggtta tagatgtctc catatcatcc tggttgatta tctgtacgat actcttagca
      301 ttactccttg cccttagtaa gaggaggtat gagttaatta atcttgaaga cgctggggtg
      361 aaacgtaagg tcctcaagga ttatgattta cagcttttag accagatgat aaacataacc
      421 acttcatctg ttcttattgc gtatacactt tatactatgt cccagaggac catcgaaaga
      481 tttgatacca gtgatcttct atttactgtg ccgttcgtcc tgtatggtct cttcagatat
      541 ctgtatctga tatatagaag aggatttggg gagagccctg aagaagcgtt tattattgac
      601 aagccattga tgatagatat tattctttgg attataatag tctttgtgat aatctatata
      661 ttgtgaggga gagcagttta tggcaagatc aactgttgtg gttttaaaga ctggtccaaa
      721 gggtgttctg gatgattatg gcagattaat gaggttatcc aggtacggtg attttgtcag
      781 aagagatatt gatattattc taaagctcaa tctctcctgg acaagatatt tcccagcatg
      841 ttcttctacc ccctggcagg tggagggtgt tataaaaaca ctcttagatg atggatatga
      901 gagggaaaga atatttccag tagagaacag aacggttgta acaaacccga taaagggagc
      961 tattaataat aagtggttac ctgtattaga gaggtacgga cttcacttta taccgctgac
     1021 tacagttaga tggattaagt atgagaccag gaccaaacta ttaaggctta atgagatttt
     1081 ccctgaaggt tttgagatac cggagatgtt tatagggaag aatattattc atctaccaac
     1141 cgttaagact catggtcatt ctataactac cgggtctata aagaatgcat ttggtggact
     1201 tctgaaagag gtcaggcatt actgccataa atatatacat gaggtccttg tcgatttaat
     1261 gatcatccag agagagattc atcctgctat atttaccgtt atggatggaa cggtctgtgg
     1321 tgatggggct ggaccaagga cgatgatccc cagaatagag aattgtatcc ttgccggttt
     1381 tgatccggtc gctatagatg cagtgagtgc caagatgatg ggttttaatc cgttagatat
     1441 accttacata ggtatgtgca acgatatgaa attaggtgtg ggggatttga gtaacataga
     1501 tataatcggt gaagatatat ccgaggtgaa tttcgggttt aaaacgaaga gaagctttgt
     1561 catatggggg gatcagatgg tgagaaaagg acctttgaga tttttagaaa agcctctttt
     1621 atattctccg ctgatggtgc tccccacact ggcatctaat atttaccatg attatatctg
     1681 gtatcctgtt atcggtagat caaggataaa ggactttatg aaaacggagt ggggagagtt
     1741 atttaagaga tattgacttg gggaggatct ataatggatt atattctaaa agagaggaaa
     1801 ttgtttttat ttgctttaat aatcagcata ttattccttt acggcagtat caatatcagt
     1861 tacaatatgt acgatgaggg tatagtagtt tatggggcaa gcagaatact taaaggagat
     1921 atgccgtaca gagatttctg gactatgtat gcccctggtc agttttatac ggtggcattt
     1981 ctatttaaga tctttggaga aaatcttttt gtcacaaggg tttacagtgc tactataaat
     2041 cttctgatag ttttgctggt atattttatt gttagaaaag tctcggacga aaggttggcg
     2101 cttctctccc tggttctttc caccctttgg atgggcggtt ggggtctgtt ttactcttct
     2161 ccaaccccag ccgggacatt ctggagtctc ttgggcatat actttgtgat tgattttctc
     2221 tctacagaga aactctcaag tctatttact ggtggtatca ttactggtat aggtgctatc
     2281 ttcaggcatg atataggtgg ttatacattt atagcttcaa ctatagtgat actgttttat
     2341 gcgtatctaa agatatctga taggagtatt aaagatacca taaaactctg gggtagatat
     2401 cttgccggta ctgtagcagg atttctgcca tttgccatct attttttagt taacgttcca
     2461 tttggagaga tcatctatga ccttataatc tttccaggta aagttttcct cagggttaga
     2521 gatttacctt atccgggttt tggatttaat ctatactctt ttcttttcta tttcccaata
     2581 ataatatatc taatttctgg gatttttata gttttaaata tatttaaatt gaaatatttt
     2641 aaagggaatg agtgggttaa tttactcttt ttgatacttg gtcttttctt ctttaatcag
     2701 gcgtgggtaa ggtcagatgt tcctcattta cttccaacca taattccagc gattatgtta
     2761 ttatcttctc tttataagag tgaacttatc aaaattctgg gaaggagaga aattattatc
     2821 aaaattcttc tccttcttat ggggtttatc cttacccttg ctctatatct taatgcttta
     2881 aagggtcttg ttatcgctgt atatgcactt cctcttgtgt tggggacggt aatatccatc
     2941 attatatata tccctgttca gttcaggaga ttctatagag atatccctgc taaggctact
     3001 ttgcaggtag tctctctggt gttatctgtt tttgtcattt tcagttgttt tagagatttt
     3061 aagggtgttt ctatccagcg catcttctct tttaagatac cagggcttgt aacatctcaa
     3121 tttgaaaggg ctaaagggat atacgtcttt caggggcagg agacttacct tgaacctgcc
     3181 attaaaatcg ttcgggcaaa cacaaaacca gacgagctta tatttgtcgg aaattcaaga
     3241 catgacagaa tctttattaa tgatataatg ttttactttc tctgtgagag acatagtgcc
     3301 acaaaatatc atgaacttca tccaggattg gctaatacca ggaaggttca gcttgaaatc
     3361 atcgatgagt taaaaaggaa taatgtgaaa attatagtct tatggaatgg ggctgagaat
     3421 gttgtagaac ccaatgagag ttcagtcagt acaggcgtaa atgacctgga tgactttatc
     3481 agattaaact atgctactat tatgtacctg ggaccatata gaatcttaca gagaataaca
     3541 ggtggtgaga taggttgatt tatatactgc ttgtactttc tcttatatcg aatgcactgg
     3601 ctaatgtatt tataaaagct gggatgagag attatactgg gggtatactt cacatatttc
     3661 atcatttgtt aaggagccct ctcgtacttg cagggctttt attttttggt cttgccttta
     3721 tcgggtacag ttttgtcctt tccagattag agctgagcat agcatatcca ataatgacag
     3781 gtgctggttt tgcaattgtc tctatattct ctttcatact gttcggtgaa agttttagtc
     3841 cggttaaaat cattggtata atctttgtct ttattggtat aatccttcta tccaggtaga
     3901 ttgtggtata attaagttaa ggttaatcca gattgacgga aggagggaag atctatgagt
     3961 gataagtcgt tgagagtagg tgttatagaa gaagtgataa gtcttccatt aaaaaataat
     4021 aggaggtggt ttgattgaaa ctaggagtat ttgctgttct gtttcaagat ctaccggtag
     4081 aagaagccct tgataagatt gtttcttatg ggctatcagc ggtagaggtg ggatgtg
//
LOCUS       DAOTKU010000101         4122 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_18404_length_4122_cov_3.343005, whole genome shotgun sequence.
ACCESSION   DAOTKU010000101 DAOTKU010000000
VERSION     DAOTKU010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4122
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18404_length_4122_cov_3.343005"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..2498)
                     /locus_tag="P5310_03440"
     CDS             complement(57..2498)
                     /locus_tag="P5310_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013430729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HRU32447.1"
                     /translation="MKRINLDRSWECLESPVSLINVLGLMKSDFNWKPVNLPHDVAIE
                     KERDKDNPSGSGEGYTAGSSLFYKKELFLDEKWRGKNLILEFEGIMGIAEVFVNGRLV
                     AKHFNGYTSFLVDITKHVNFDDKNTIFVHVENTHKPNSRWYAGTGIYRHVWLYVGNKV
                     YIKPWQLHVETKAIDDGTAILEIRSAVANSSSETASRVMRFQIFSASGELLLSNKQGF
                     DIGRNEGKEIIKTLELKPFRYWDIEDPYLYEIQADIIRDEDIEDSASTMFGIRTISVS
                     PKEGLKLNGKSIKLKGGCTHHDNGPLGSASYDRAEERKIELLKASGFNAVRLTHNPFS
                     PGFLDACDRLGMLVIEEFFDVWTGGKVSFDYHLFFDKYWEEDIENTIKRDYNHPSLIM
                     WSIGNEITWGAGIDVNDKDGYSNIYTWCRRLSLKVKAMDSTRPVTSAFCAIPSELINR
                     SSLEDGNIVVTMLPPEEVDPANDKWGEVTEIFFEPLDVAGYNYKVGRYSYDSVRFPNR
                     IICGTETHPYTLFKNWTETVKNPNVIGDFVWTAIDYLGEAGIGRFWLDGNGMKMFGGE
                     FPWLLANCGDIDICGEKRPQSFYRDIIWGNRSDPYIAILPPNLYGKKLYIRAWGWEPV
                     ERSYTFPGCEGMPITVYVYTDADEVELFINGRSLGRKTSGFNTQFKTTYDATYEPGVI
                     EVVAYKNGIEIGRDRMESASEPVSIKLLPDRDIISSSYNDLCYIKIIAVDKNGKESPY
                     ADNRITVEVDGAGEFVVLGTADPLSTELFVNRERKLYKGKALAIVKSIGEKGEISFKA
                     KGEGLEGAQIAIKCI"
     gene            complement(2524..2868)
                     /locus_tag="P5310_03445"
     CDS             complement(2524..2868)
                     /locus_tag="P5310_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014769353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HRU32448.1"
                     /translation="MKRNYKLFLKDILEAMESIEKFTEGMSFEEFKTNDLVSSAVIRK
                     FEIIGEATKQIPQDIRNKYLDIPWKEMAGMRDKLIHFYFGINHELVWKTVKNRIPQIK
                     SLIKKILIELDE"
     gene            complement(2865..3155)
                     /locus_tag="P5310_03450"
     CDS             complement(2865..3155)
                     /locus_tag="P5310_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013467327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HRU32449.1"
                     /translation="MKKEDILDILRSLQIEIEEKYNAEVIGIFGSFVRGEEKETSDID
                     VLVDFNESADLFDLVGLSLFLEEKLHRKVDVVPIDGIREELKETILKEVVYT"
     gene            complement(3380..>4122)
                     /locus_tag="P5310_03455"
     CDS             complement(3380..>4122)
                     /locus_tag="P5310_03455"
                     /inference="COORDINATES: protein motif:HMM:NF013377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HRU32450.1"
                     /translation="LYWYEDSLPIDSRRWESGYDLSPRWDYVENGPFPCIDINCQMAL
                     YFQCVSHLSYLCNDSVTAYRFKEEYLSLKSLIRENLLNKELKIFTDNNSDSRNAKKTI
                     AMFWSLVSGVAEDEEIEYMVRLLEDKDEFCAPYGLPTVSMSESSFELDCWRGPMWMSQ
                     VFWVALGLQRYGLIKKASWLAQRSLEVVRKVSEDTGKIWEFYNPIDGDMGKLRRKGRN
                     TGPFPDYLGHNPLLSLERIAEEESICPF"
BASE COUNT         1204 a          951 c          576 g         1391 t
ORIGIN      
        1 ttgatttttt gatggagggt gtcagactac ctgcaacttt cagtttctct ttcttatcag
       61 atacatttta tggcaatctg tgcaccctcc agtccttctc ctttagcttt aaagctaatt
      121 tctccctttt ccccgatgct ttttactatt gctagtgctt tccccttgta gagctttctc
      181 tctcgattaa caaataattc agtcgataac ggatcagctg tccctaaaac tacaaattcc
      241 ccagccccat caacctcaac agttatccta ttatctgcat atgggctctc ttttccgttc
      301 ttatctacag caattatctt tatatagcac agatcattat aggaagaaga tatgatatct
      361 ctatcaggta aaagttttat tgacactggt tcactagctg attccatcct gtctctaccg
      421 atctctatac catttttgta tgctactacc tctattactc ctggctcata ggtagcatca
      481 taagtcgttt taaactgagt attaaaccca gatgtctttc ttcctaaact tcgaccattt
      541 ataaataatt cgacctcatc agcatcagta taaacataga ctgttatagg catcccttca
      601 catcctggga atgtataact acgttcaacc ggctcccatc cccaagctct tatgtataac
      661 tttttaccat aaaggttggg aggaagtatc gcaatataag gatcacttct gtttccccaa
      721 ataatatccc tataaaatga ctgtggacgt ttttccccac agatatcaat atcgccacaa
      781 tttgctaaaa gccatggaaa ttctccacca aacatcttca tcccgtttcc atctaaccag
      841 aatcttccaa tcccagcctc tcctaaatag tcaatagctg tccatacaaa atcgccaatt
      901 acattgggat ttttcaccgt ttctgtccag ttcttaaata gcgtataggg gtgtgtctca
      961 gtaccgcata tgatacgatt tggaaatctt acagagtcat atgaatacct tcccaccttg
     1021 taattatagc cagctacatc taacggttca aagaagattt cagtgacttc cccccactta
     1081 tcattagcag gatctacctc ttctgggggt aacatggtta ccacaatatt tccatcttca
     1141 agtgaactcc tattaatgag ttcggaaggg atagcacaga aagctgaagt cactggtctt
     1201 gttgaatcca tagcttttac ttttagcgat aaacgtctac accaggtgta tatgttagaa
     1261 tagccatctt tatcattcac atcaattcca gctccccagg tgatttcatt cccgatagac
     1321 cacataataa gagatggatg attataatcc cttttaatag tattttctat atcctcttcc
     1381 cagtatttat caaagaatag atgataatca aaactcacct tgcctcctgt ccacacatca
     1441 aaaaattcct caattactag catccctaat ctgtcacagg catctaaaaa tccaggtgaa
     1501 aaagggttat gtgttagtct taccgcattg aacccggagg cttttaaaag ttctatcttc
     1561 ctctcttctg ccctgtcgta gcttgcactt ccaagaggtc cattgtcatg gtgggtacat
     1621 ccacccttca actttattga ttttccgttt agtttaagtc cttccttcgg actaactgaa
     1681 attgttctaa tcccaaacat cgtagaggca gaatcctcaa tatcctcatc ccttataata
     1741 tctgcctgaa tctcatacag ataagggtct tctatatccc agtatctgaa tggttttagc
     1801 tctaaggttt taattatctc tttcccttca tttctaccaa tatcaaagcc ctgtttgtta
     1861 ctaagtagta gttcaccgct agctgagaat atctgaaatc tcataactct gctggcagtc
     1921 tcactagagc tattagccac tgctgatcta atctctaata tcgcagttcc atcatcaata
     1981 gctttggttt caacatgcaa ctgccatggt tttatgtaca ctttgttacc aacatagagc
     2041 catacatgtc tatagatacc agtgcctgca taccatcttg aatttggttt atgagtattc
     2101 tctacgtgaa cgaagatagt atttttatca tcaaaattta catgttttgt tatatccact
     2161 aagaaacttg tatatccatt aaaatgttta gcaactaatc taccatttac aaagacctca
     2221 gctatcccca taattccttc aaattcaagt atcaggtttt tacctcgcca cttttcatcc
     2281 aagaataact cctttttata gaacaggctg ctcccagcag tatagccctc gccagaacca
     2341 gatggattat ctttatccct ttccttttca attgctacat cgtgtggtag atttacaggc
     2401 ttccagttaa aatctgactt catcaagcct aatacattaa tcaatgaaac agggctctct
     2461 aaacattccc atgatctgtc aagattaatt cttttcatat aaaacctccc tggagattat
     2521 attttattca tctaattcta tcagtatctt ctttattaaa gactttatct gagggattct
     2581 atttttgaca gtcttccaaa caagttcgtg gttaatacca aaataaaaat ggattaactt
     2641 atctctcatc cctgccatct ctttccatgg gatatcgaga tatttattcc gtatatcctg
     2701 aggtatttgt tttgtagctt ctcctattat ttcaaatttt ctgataaccg cacttgatac
     2761 aaggtcattt gttttgaatt cttcaaaaga catcccttct gtaaattttt ctatgctctc
     2821 catagcttcg agtatatcct taagaaatag tttatagttc cttttcatgt ataaactact
     2881 tctttgagga tagtttcttt taattcttct cgtatcccat caatagggac aacatctacc
     2941 tttctgtgta gtttctcctc aagaaaaaga gaaagcccaa caaggtcaaa taaatctgcc
     3001 gattcattaa aatccacaag cacatctata tcacttgtct ctttctcctc tcctcttaca
     3061 aaagagccaa agatacctat aacctctgca ttatatttct cttctatctc tatctgcaag
     3121 cttctcaaga tatcgagtat atcctctttt ttcatacctt taccaccctt atattcaagt
     3181 tatatctgaa actttcatag atattttaac atggtattta agattcatac catctgatta
     3241 tcccattata aagaaatcct ccaagagtct ttttgtctga ctgaatgata ttacacagga
     3301 gatagaaagt caaggaattt cagcagccgt atttgatatt tttggatatc agaattatgc
     3361 tgaaggaaaa actctttaac tagaagggac agatagattc ttcctcggct attctttcaa
     3421 gacttaagag aggattatgt cctagatagt ctgggaatgg accagtattt cttcccttac
     3481 gacgtagttt tcccatatct ccatcgatag gattataaaa ttcccatatc ttaccagtat
     3541 cttctgaaac cttcctaaca acctcaaggg aacgttgagc taaccaggaa gccttcttaa
     3601 ttaatccata tctttgtaaa cctagagcca cccagaatac ctgagacatc cacataggac
     3661 ctctccaaca atcaagttca aagctagatt cactcataga tacagtaggt aatccatatg
     3721 gtgcacaaaa ttcatcctta tcttcaagaa gtcttaccat atattctatt tcttcatcct
     3781 ctgccactcc acttactaaa gaccaaaaca tagcgatagt tttcttcgca tttctactat
     3841 ccgaattatt atcagtaaat atctttagtt ccttatttaa aagattctct ctaatcaagc
     3901 tcttaagtga taaatattcc tctttaaatc tataagcagt aactgaatca ttacataaat
     3961 aagaaagatg gcttacacat tggaaataga gcgccatctg acaattgatg tctatacagg
     4021 ggaatggtcc attttctaca tagtcccagc gtggagatag atcatatccc gactcccacc
     4081 tcctcgagtc aataggaaga gaatcttcat accaatagag tt
//
LOCUS       DAOTKU010000102         4090 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_18568_length_4090_cov_3.652788, whole genome shotgun sequence.
ACCESSION   DAOTKU010000102 DAOTKU010000000
VERSION     DAOTKU010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4090
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18568_length_4090_cov_3.652788"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1535
                     /locus_tag="P5310_03460"
     CDS             <1..1535
                     /locus_tag="P5310_03460"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRU32451.1"
                     /translation="YLRKGLKWSDGEPFTADDIMFWYKDILLNKELTPVPPKWLTSEE
                     EVAKVEKLDNYTVRFSFKKPAGLFLPMLATTSGRNLITPSHYLKQFHPNYVTKDKLEE
                     LIKKEKLNTWINLFSNKNDPWMNPELPVLNAWKVVTPIGTGTRVSLERNPYYWKVDTA
                     GNQLPYIDRVTADIVESGEVSKMKALNGEIDMQSRGIGQNIADYPLFMENREKGDYRL
                     IRQTHTSMNQHTIIFNLNHKDPVLRKLFNDRRFRIALSHAINRQEIIDLVFLGLAEPW
                     QISPQRGTPLFHEAAAKNYTEYDPKKANQLLDEIGLTKRDRDGFRLRPDGKTLEITIE
                     ISGIPGDIWEMVRKYWQAVGIKVAVKGEDRTLWDIRTAAAEYDVTSWWGDSGTGYEPL
                     IMPYWYFPWMYPDSRQAPQWALWYQSGGKSGEVPPPEVRHQMALYDQITVATDPAKQN
                     QLFKQILDVQAKNLYVMGICTIPPQFIITKNNFRNVPEAYESGQFPTHAPVNTCQFFI
                     KK"
     gene            1592..3610
                     /locus_tag="P5310_03465"
     CDS             1592..3610
                     /locus_tag="P5310_03465"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HRU32452.1"
                     /translation="MTDILEIIKNPDRVELILSRENGKKVYLRITSSKANVWRLQSSD
                     VKGGFEDLGAVQLLKEEFNEPSNENILPLDVKEKSDTSFQIESAVNSTSIQINLSPFH
                     ILFLDKTGKTKCTIDDISFDEGSITISGSLKNNEFTYGLGERFNGANQRGKSAHIWSE
                     DRWCQTEGNSYVPIPFVLSTEGYALFMNRYEYSFFDLGENTPDRWSITLKDAPLDLYV
                     FIEDSPKEILRDYAELSGFSPMPPEWSFGIFVSRHGRLREFATIEGIREMARKMEEND
                     LPWTTVIIEGWNTYDTSTYEDLKKIVDELHKKGKKVLVYERCGRLDKEYWEAHKAKED
                     FFVKDSSGNTLIKEAPLYNPVDAPDRRLSCFLDLTNPETLDWWQNYVWKRLLVDIGLD
                     GAKIDFGEEFPEDENLKLFSGRSIKGMHHYHAVKYGTMMYNLYQKMRPEGGICWARGG
                     GIGAQRYPYLWCGDQLREFRFLKAILSAILSSGVSGIPFMCHDLAGYMPARDKERNIE
                     EEVFIRGTELACFTVNMETHGTVTRPYDFSPEIIDIYRLYSKIHYALIPYLIEQARIS
                     SGTGVPIVRHLYLEFPEDEKVRGIEDEYMLGDGLLVAPVLEDSNKRDIYLPSGEWNDL
                     WNEKIFKGPYEFSKYTAPLERIPIFIYSDTRSEVLDKVVENIRSILKS"
BASE COUNT         1358 a          686 c          901 g         1145 t
ORIGIN      
        1 tctatctccg aaaaggatta aagtggtctg atggagagcc attcacagct gacgatatta
       61 tgttctggta taaagatatc ttactaaata aagaactgac tcctgttcca ccaaaatggc
      121 tcaccagtga agaggaagta gcaaaggtag aaaaacttga taattacact gttagattca
      181 gctttaaaaa gcctgcagga ctctttctac caatgctcgc cacaacctca ggaagaaatt
      241 taatcacccc ttctcactat cttaaacaat ttcatccaaa ctatgtgact aaagacaagc
      301 tagaagaact tataaagaaa gaaaaactca atacctggat caacttgttt agcaacaaaa
      361 atgacccctg gatgaatccg gaactccctg tcttaaatgc ctggaaggtt gttaccccca
      421 ttggtacagg tacaagggtt tctcttgaga gaaatcctta ttactggaag gttgatacag
      481 caggaaatca acttccatat atagacaggg tcaccgctga tatagtggaa agtggtgagg
      541 tttcgaagat gaaagccctt aatggagaaa tagatatgca aagtaggggg attggacaga
      601 acatagctga ctatcctctc tttatggaga atagagaaaa aggggattat agacttataa
      661 ggcagacgca tacttctatg aatcagcaca caataatatt taacctaaat cataaagacc
      721 ctgtcctaag gaaactattt aatgatagga gattcagaat agccctttct catgctataa
      781 acagacagga aattatagac ttggtctttt taggactagc tgaaccttgg cagatatctc
      841 cgcaaagggg aactccactt ttccatgagg ccgctgctaa gaattatacc gaatatgacc
      901 ctaagaaggc caatcaactc ttagacgaga taggtctaac taaaagagat agggatggtt
      961 ttaggctgcg tccagatgga aagactctag agataactat agaaatatct ggtattcctg
     1021 gggatatttg ggagatggtt agaaaatatt ggcaggcggt cgggataaaa gtagcagtaa
     1081 aaggagaaga cagaactcta tgggatataa ggactgccgc cgctgagtat gatgttacaa
     1141 gttggtgggg agattctgga acaggatatg agccattaat aatgccatac tggtatttcc
     1201 catggatgta tccagattcg agacaggcgc cccaatgggc actttggtac caatctggtg
     1261 ggaaatctgg agaagttcca cctccagagg taagacatca aatggctctt tatgatcaga
     1321 ttacagttgc tacagatcca gcaaaacaaa atcagctatt taagcagatc cttgatgtgc
     1381 aggctaagaa tctctatgtg atgggtatat gtacgattcc acctcaattt ataattacta
     1441 agaacaattt taggaatgtc ccggaggctt atgagagtgg gcaattccca actcatgctc
     1501 ctgttaacac ctgtcaattc tttataaaga agtaaatatc acaaaggggg gcatctttta
     1561 gatgcccccc ttataatagg aggtaagata tatgacagat attctggaga tcataaaaaa
     1621 ccctgataga gttgagttga ttctttccag agagaatggg aaaaaagtct atcttaggat
     1681 tacatcctct aaagctaatg tatggaggtt acagagttca gatgttaaag gaggattcga
     1741 agatttagga gcagtccagc ttttaaagga agaatttaat gaaccatcga atgaaaatat
     1801 cttaccctta gatgtaaaag aaaaatctga taccagtttt cagatagaga gtgctgtaaa
     1861 tagtacatct atacaaatta atttatctcc ctttcatatt ctttttttag ataaaacagg
     1921 aaaaactaaa tgcacaatag atgatatatc ttttgatgaa ggttctatta ccatctctgg
     1981 ttcattaaaa aacaatgaat ttacttatgg gttaggagaa agattcaatg gtgctaacca
     2041 gagaggaaaa tctgctcata tatggtctga agatagatgg tgtcagacag aaggtaacag
     2101 ctatgtaccg attccatttg ttctgagtac agaaggatat gcccttttca tgaatcgtta
     2161 tgagtattca ttttttgatt tgggagaaaa tactcctgat agatggtcca tcactcttaa
     2221 ggacgccccg ttagatctgt atgtatttat cgaagattct cctaaggaga ttctaaggga
     2281 ctatgctgaa ctatcagggt tctcacctat gcctccagaa tggagtttcg gaatctttgt
     2341 tagcagacat ggaagattgc gagagtttgc cactattgaa ggaattagag agatggcaag
     2401 gaagatggaa gagaatgatc tgccctggac tactgtaatt attgaagggt ggaacaccta
     2461 tgacacatca acctatgaag atctgaaaaa gattgtcgat gaacttcata aaaaggggaa
     2521 aaaggtcctc gtctatgaga gatgtggaag acttgataaa gaatactggg aagctcataa
     2581 ggcaaaggaa gacttctttg tcaaagatag ttcggggaat actcttatta aagaagctcc
     2641 actttataat cctgttgatg cgccagatag aagattaagc tgctttttag accttacaaa
     2701 tcctgagacc ttagattggt ggcaaaatta tgtatggaag agactcctag tagacatagg
     2761 attggatgga gcaaagattg actttggtga agaatttccc gaggatgaga atcttaagct
     2821 tttttcggga aggtctatta aaggaatgca ccattatcat gctgtgaaat atggtacaat
     2881 gatgtataac ctctaccaga agatgcgtcc agaaggtgga atctgctggg ctagaggagg
     2941 aggaattggt gctcagagat atccgtactt atggtgtgga gaccagttaa gggaatttcg
     3001 ctttttaaag gctatcctgt ccgccatatt gagttctggg gtctctggaa ttccatttat
     3061 gtgtcatgac ctggctggtt atatgccagc aagagataaa gaaaggaaca tagaagaaga
     3121 ggtctttata agggggacag aattggcgtg tttcactgta aatatggaaa cacatggcac
     3181 agtaacaaga ccttatgact tctctcctga aataatagat atctacaggc tttattcaaa
     3241 gattcattat gcattgattc catatctgat agagcaagcc cgtatttcct ccggtactgg
     3301 agtccccatt gtaagacatc tgtacctaga attccctgaa gatgagaaag ttagagggat
     3361 cgaagatgag tatatgcttg gagatggatt attagttgcc cctgtactcg aagattccaa
     3421 taaacgcgat atttatttac cttctgggga atggaatgac ctgtggaacg agaaaatatt
     3481 taaaggtcca tatgaattct ccaagtatac tgccccgcta gagcgtatac ctatctttat
     3541 ttattctgac acaagatcag aagttctaga taaggtggtg gagaatataa gatcgatatt
     3601 aaagtcatag cagaagtaag ttacgctagc caatcatttc caacgattaa tatcgaaagg
     3661 ttcttgagaa agaggcttat ctactctata atagatagca tctatatcct gtacgaagtt
     3721 aagtatccct ctttatgggg taccttatta tcaaaaatta ggtatacctt tacttataag
     3781 tttccagagt agtttatggt acattgaggc ttgaaaggta atgcaggcac acatcgatag
     3841 atgaacatag aatgaaaggg gtgatgcgtg aaaagaagag catagaagac atcttgtcat
     3901 aaataatatt cgtagggatc ggatgtactc tgaggataag gattatctac cctttacaag
     3961 gaggatgaaa tgaaagaatt aaaaggtttg agcgatttat tagaaactgc aagaagtata
     4021 cttcccttag ttttcactct gattttcttt cagctggtgg tattaaagaa acccatcgaa
     4081 aatgttaagg
//
LOCUS       DAOTKU010000103         4065 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_18697_length_4065_cov_3.945387, whole genome shotgun sequence.
ACCESSION   DAOTKU010000103 DAOTKU010000000
VERSION     DAOTKU010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4065
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18697_length_4065_cov_3.945387"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            26..1162
                     /locus_tag="P5310_03470"
     CDS             26..1162
                     /locus_tag="P5310_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013296781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRU32453.1"
                     /translation="MQYRKFGRLDFNVSALGFGCMRLPIIGGDEGNVDEDLTIKMIRY
                     AIDNGVNYVDTAYGYHRGKSEIVVGKALKDGYRERTMLATKLPVWHVNAREDMDRLLN
                     EQLKKLDTDHIDFYLLHSLHKDTWDKVYNLGVLDWIESIIKRGLIRHIGFSFHDDLDT
                     FKRIVDSYDWDFCQIQYNYLDIEHQAGMAGLKYASNKGLGVVVMEPLRGGKLANPPDR
                     VREVFDSFPIKRSPVAWALLWLWNQPEVSTVLSGMSAFEQVKENIEIADKSGINILTE
                     EELSLIERVREAYLGFKAIDCNECGYCMPCPNGVDIPKNFHLYNEGVRYNDFNGPRWH
                     YRNEMQESERASSCIKCGTCEPQCPQHLPIRELLDKVKAAFENA"
     gene            complement(1199..2635)
                     /locus_tag="P5310_03475"
     CDS             complement(1199..2635)
                     /locus_tag="P5310_03475"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRU32454.1"
                     /translation="MSKKIPYGRFILLTVLIISGILMGSIAYSYVSRCICAINYNQPL
                     DYGEGPILNQVRYILQGRNYYKDINSPPYIIGNYPPVFNLLSLLLVPIFGVSFATGRF
                     LAFLAVIGTSYIIYKIVLKFTENKMIAFLTALFYLAIDYVYVWGIYMRIDTLSIFFNM
                     LGIYWVIQYEDNHKKLRWCILFFLLALYTRQSMIAGVAGAYIYLLTKDRKLAVKLIGH
                     TALYGLLTFIFLNIITKGQFYMHIVKYNINTFTIRQLWFFLSKELFTYTTLIIISLIA
                     SIFWLKDQKRRIISLYFIFAFLVQFTAGKVGSMLNYLIEPVGVMGIIFGIIIGDTLKK
                     KNQLINTGVAVLVLLQLANYYYGPNTLSWGWKPSPPLTALDRYVEESKGLILAENLGI
                     LPAHNRDIYFDPFIFTQLYYQGIWDQSKIIKDIEGKKFDIIMLEFDLYYDHWTDSDRW
                     SKEMKQAMYENYYLIDTQGYIRGYAPIR"
     gene            2736..3487
                     /locus_tag="P5310_03480"
                     /pseudo
     CDS             2736..3487
                     /locus_tag="P5310_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003382615.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
     gene            complement(3535..>4065)
                     /locus_tag="P5310_03485"
     CDS             complement(3535..>4065)
                     /locus_tag="P5310_03485"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRU32455.1"
                     /translation="KLLLSMGMEPSAQVILAEPTFKEVKIDIENLKKQAQNNLPTVIQ
                     ARNGYNLAKLQYDLANNEYTPQSQIRSAQLSLASAQDSLKQVIDNLDTTIDSAYNNVV
                     NTLNQIKVQEKNLDIARKNLDTDTARLKVGTITKLQLMSTESSLISAENNYKSAVYNY
                     LKALDSLSLAVGAPVF"
BASE COUNT         1271 a          698 c          865 g         1231 t
ORIGIN      
        1 attctacctt aacaaggagg tctatatgca gtataggaag tttggaagat tagactttaa
       61 cgtgtccgct ttaggtttcg gatgtatgcg attgcctatc attggagggg atgagggcaa
      121 tgtagatgaa gacctgacaa taaagatgat aagatacgcc attgataatg gagtaaacta
      181 tgtagatacc gcttatggat atcacagagg gaaaagtgag atcgtagtag gaaaagcatt
      241 gaaagacggt tatagagaga ggactatgct ggcaaccaag ttaccggtct ggcatgtaaa
      301 tgcaagagaa gatatggata gacttctaaa tgaacagctg aagaaacttg atacagacca
      361 tatagatttc tatctattac attcactgca taaagatacc tgggacaagg tatataatct
      421 tggagtcctg gattggattg aaagtattat caaaagggga ctgataagac atataggatt
      481 ttctttccat gatgatctgg acacatttaa acgtattgtt gattcttacg actgggactt
      541 ttgtcagatt cagtataact atctagatat agaacatcag gcgggtatgg ctggtcttaa
      601 gtatgcaagt aataagggtt tgggagttgt ggtaatggaa cctcttagag gtggaaagtt
      661 agccaatcct cctgataggg taagggaggt atttgatagt tttccgataa aaagaagccc
      721 ggttgcctgg gctcttctct ggctctggaa tcagcctgag gtttctacag tgctgagcgg
      781 tatgagcgct tttgaacagg ttaaagagaa tattgagata gcagataagt ccggaataaa
      841 catacttact gaggaagaac tgagcctgat agagagagtg agagaggctt atcttggctt
      901 caaggctata gattgtaatg agtgtggata ctgtatgccc tgtcccaatg gtgttgatat
      961 acccaaaaac ttccatcttt ataatgaagg ggtaagatat aatgacttca atggacctag
     1021 atggcattat agaaatgaga tgcaggagtc tgagagggca agtagctgta taaaatgtgg
     1081 gacatgcgag ccacagtgtc ctcaacattt accgattaga gaactgctgg ataaggtaaa
     1141 agctgcattc gagaatgcat aattgaaaaa ctcggggagg actctcctcc cttatttttt
     1201 atcttatcgg cgcgtagccc cttatataac cctgagtatc gataagatag tagttttcat
     1261 acatagcctg tttcatctct ttagaccacc tgtcagagtc agtccagtgg tcatagtata
     1321 aatcgaactc cagcataatg atatcaaact ttttcccctc tatgtctttt attattttac
     1381 tctgatccca tataccctga tagtatagct gggtaaatat gaatggatca aagtatatgt
     1441 ctctattgtg tgcaggcagt atacccagat tctctgccag aataagccct ttggattctt
     1501 ctacatatct atcaagagca gttaaaggag gggatggttt ccaaccccag cttagcgtat
     1561 ttggtccata ataatagttg gctaactgca gtaataccag aacagcaacg cctgtattga
     1621 ttagctgatt tttcttcttg agtgtatcac ctataattat accaaagatt atacccatta
     1681 cccctaccgg ttcaataaga tagttaagca tagaaccaac ctttcctgcg gtaaactgta
     1741 ccagaaaggc gaagatgaag tacaggctga ttattctcct cttctgatcc tttaaccaga
     1801 agattgaagc gattaagctt ataattatga gagtggtgta tgtgaacaac tcttttgaca
     1861 ggaagaacca gagctgtctg atggtgaatg tattgatgtt atattttact atgtgcatat
     1921 agaattgtcc ttttgttatg atgttaagaa agataaacgt tagcagacca tacagtgcgg
     1981 tgtgtcctat aagtttaacc gctaactttc tatcctttgt cagcagatat atgtaggcac
     2041 cagcaactcc agcaatcata gattgtctgg tatagagggc aagtaaaaag aacaatatac
     2101 accatctgag ttttttatgg ttatcttcgt attgtatgac ccagtatatc ccaagcatat
     2161 tgaagaatat agatagggta tctattctca tatagatccc ccacacataa acgtaatcta
     2221 tagccagata gaacagggca gttagaaatg ctatcatctt gttctctgta aacttcagga
     2281 cgattttata gattatatag gaggttccta taactgccag aaatgccagg aaccttcctg
     2341 tggcgaatga tactccaaag attggaacaa gtaataggga gagcagatta aaaactggtg
     2401 ggtaattccc tataatatag ggcggagagt ttatatcctt gtaataattc ctcccctgca
     2461 atatgtatct tacctgattt agtattggac cttccccata atccaggggt tgattatagt
     2521 tgattgcaca gatacatcta ctcacataag agtatgctat agaccccatc aagataccag
     2581 agatgattaa aactgtaaga aggataaacc ttccgtatgg aatctttttg gacaatctct
     2641 caccccgcta tgttaagttt tacaatcttt ctataaaatc ctattataat ataatagata
     2701 ataagacaaa agttaagtct tttggaggta agtggatgag tcctttagaa aaagcggtgg
     2761 agtttataaa gtcttctaac aatatagtgg cttttaccgg agcgggggca tcaactgaga
     2821 gtaatatacc tgattttaga tcagatcagg gtctttacag caaggaaaaa gaagaatatg
     2881 gatatccgcc tgaagtaatg ctcagtcata gtttctttat gagacatcca gataaattct
     2941 atacattcta taagaaggag attgtacata gggatgcgga gccaaatccc tgtcacctgg
     3001 cattagccaa attggaagag ataggaaagt taaaagcgat tataacccag aatatagatg
     3061 gattgcatca aagggcaggt tccagaaatg tcatggaact acatggaacc atccataaaa
     3121 attactgtct atcctgtgga aaattctttg acctaactat atcttagata gtaaagaatt
     3181 ggtacctaaa tgtgatgagt gtaatggaat tataagacct gatattgttc tgtatgaaga
     3241 gcctctggat tctgatgtat taaatgattc ggtaacctat ataagtgagg cagattgttt
     3301 aattgtcata ggtacgagtc ttgttgtata tccggcagct gcacttatag attactataa
     3361 gcaggagaaa atgataataa taaataagac cccaacaccg tatgattgga aggcaagcgt
     3421 tataattaga gataatgctg gagttactct gaagaagata ttgaaggagc tgggtatctg
     3481 ggaatgaaat agaaagacgg ggttttaatt cctaaccccg tcttaatcta tagcctaaaa
     3541 tactggtgca cctactgcaa gcgacaaact atccagtgct tttagatagt tatatactgc
     3601 cgatttataa ttattctctg cacttatcag agatgactct gtactcatca gttggagctt
     3661 agtgattgtt cctaccttga gcctggcggt atcagtatct aaatttttcc tggcaatgtc
     3721 cagattcttt tcctgaactt ttatctgatt taaagtattg accacattat tgtaagcaga
     3781 atctatagta gtgtctaaat tatctatcac ctgttttaaa gaatcctgag cactggctaa
     3841 agatagctga gcacttctta tctgactctg tggggtatat tcgttgttag ccagatcata
     3901 ctgaagttta gccaggttat agccattcct ggcttgaatt actgtaggca gattattctg
     3961 agcttgcttc tttagatttt cgatatctat cttcacctct ttaaatgttg gttcagcaag
     4021 gataacctgt gcagatggtt ccatccccat cgataatagc agttt
//
LOCUS       DAOTKU010000104         4060 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_18721_length_4060_cov_3.453184, whole genome shotgun sequence.
ACCESSION   DAOTKU010000104 DAOTKU010000000
VERSION     DAOTKU010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4060
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18721_length_4060_cov_3.453184"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>790)
                     /locus_tag="P5310_03490"
     CDS             complement(<1..>790)
                     /locus_tag="P5310_03490"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HRU32456.1"
                     /translation="PWFARGYEYYINPKSKSLHHIRAEEITYEAINWLKAHCNEDFFL
                     FLHYWDPHSPYLPPDRYKDIFYRGKDPFDPDNKSMETARKQIPYPFFKRWHYDLLGNP
                     TDADYIIAQYDGEIRYVDDSIGELLEYLRSLGIYEDTLIILTSDHGENMTEHNFFFDH
                     QGLYDATIHIPLIIKIEDKFRGRKVSPLVQNIDIMPTILDLLDMEKDINLDGQSLIPL
                     INSECEYLYREIISTECTWRAGISIRTEGWKFIKTIDQGLYEKPS"
     assembly_gap    791..800
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<801..1105)
                     /locus_tag="P5310_03495"
     CDS             complement(<801..1105)
                     /locus_tag="P5310_03495"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRU32457.1"
                     /translation="MKILLIDIDTLRADHLGCYGYGVNTSPNIDRLSQDGVVFLNAFA
                     AGIPTTPSHTSIFTGLHSITHRIVAHGSDSIIDPDIPLLPEILRDNGYRTAAVDNLY"
     gene            complement(1122..1952)
                     /locus_tag="P5310_03500"
     CDS             complement(1122..1952)
                     /locus_tag="P5310_03500"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRU32458.1"
                     /translation="MRLAEFKHDLPFHIVIVILVFISIYPFIFMLISSFKTTEEVFHN
                     FFGLPSSLEYRNYLDAWKEVSPFLLNTIIVSVITMFGVLVLSSIDAYIFTRFDFPGKD
                     ILFFLILSLLMIPGILTLVPRFLVVRDLKLLNTYWALILPYIAGGQVMPIFILRSFFS
                     SIPKELFESAKIDGASETQCFLKITIPLSKSILFTVGLMNFLNIWNEYIWPLVTVTEP
                     KLFPITVGLVVFQSRYAGSAAWGPLFAGYTIASIPIVILFLFTMKYYIAGITSGALKA
                     "
     gene            complement(1952..2848)
                     /locus_tag="P5310_03505"
     CDS             complement(1952..2848)
                     /locus_tag="P5310_03505"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRU32459.1"
                     /translation="MKKLFREIIKNRNCYLLLLPTFILLFVFEYYPALSAVYYSFFEW
                     DGFTPPRYVGLENFIQLIFKDTVMAISAKNITILTLFRVIIAITVPLFAAELIFGIFN
                     TKMAYIYRTLLVIPMVVPSMVNLLVWKFVYNPQYGLLNQFLKAIGLSNLCRAWLGDFS
                     TALYAILFLGFPWISSLWFLIFLAGLQNISTDIFDAAKIDGVTGLKRIRFIDIPLILG
                     QIKLILILSTINALQGYVGIMVLTNGGPGNSTMVPGLYLYNNAFYFNKMGYACSIGMI
                     LFSILLVLTYINLKYIRSSEEG"
     gene            complement(2866..>4060)
                     /locus_tag="P5310_03510"
     CDS             complement(2866..>4060)
                     /locus_tag="P5310_03510"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRU32460.1"
                     /translation="LTGGTAPEISEQLFSEVNRNAYKGWYVDLTSFLEKPNPYVKDNK
                     KWKDIFLEIAIATGKAPDGKSQYVLPTGITGTAIFYNKDIFKKVGVRPPSTWKEFMDI
                     QEKIKRAGYIPFAFPMAGEKMRANWSLRTIQDMILDSKMAEIKGVKGKIKRTAIEASA
                     ISQKELVQAIKRGVYSARDPQWQEQLRLFKEWSKYWEDGYLGVDSQGAYMLFINGQAA
                     MMYESAAQIKPVEVDPLRKFEYGLFYFPRITKESSPYATGISAAAVGGVTGAGSWIIP
                     YTTSNKRLVDETIDWLMFITAPQNYIPMANDLGAAAPALKARKGLDKRLEPFVKSLER
                     GVFRIESFYRGLTREYAEAFYQILQQYLADKLTLQQACEQIQVEMEKAANALIDTHPE
                     WGIKK"
BASE COUNT         1166 a          859 c          797 g         1228 t
ORIGIN      
        1 ggatggtttc tcatataaac cctggtctat cgtctttata aacttccatc cttctgtccg
       61 gatagatatt ccagctctcc aggtacattc tgtggagatg atttccctgt atagatattc
      121 acattcacta tttatcagtg ggataaggga ttgaccgtct agatttatat ccttttccat
      181 atccagcagg tccagaatgg ttggcattat atctatattc tgaacaaggg gagagacctt
      241 tctacctcta aacttatctt cgatttttat tataagtggt atatgaatgg tagcgtcata
      301 cagtccctga tggtcaaaga aaaagttatg ctctgtcata ttctctccat ggtctgaggt
      361 gagaattatc agtgtatcct cgtatatacc cagagacctg agatattcca gcaattctcc
      421 tatcgaatca tcaacgtatc ttatctctcc atcatactgg gctattatat agtctgcatc
      481 tgttggatta cccaataaat cataatgcca tctcttaaag aaggggtatg gtatctgctt
      541 tcttgcagtc tccatactct tattatccgg atcaaacggg tctttacccc tgtaaaatat
      601 atctttgtat ctatctggag gtagatatgg tgagtgaggg tcccagtagt gcaggaagag
      661 gaaaaaatcc tcattacaat gagctttaag ccaatttata gcttcgtaag ttatctcttc
      721 agctcttata tgatggagac ttttcgattt gggatttata tagtactcat acccccttgc
      781 aaaccatggt nnnnnnnnnn ttgtacaggt tgtcgaccgc tgcagttctg tacccattat
      841 cccttagaat ctccggtagt agagggatat ctggatcaat gattgagtct gaaccgtgag
      901 caactatcct gtgagttata ctgtgcaaac cggtaaatat gctggtatga gatggggtag
      961 tgggaatacc agctgcaaaa gcgttgagaa agaccacacc atcctgtgat agtctatcta
     1021 tgtttgggct tgtattgacc ccatacccat agcaaccaag gtgatcagcc ctcagtgtat
     1081 ctatatcaat taatagaatc ttcatctgtc tcctcccatt cttatgcctt tagggcaccg
     1141 cttgtaatac ctgcaatgta gtacttcata gtgaagagaa acagtattac tatggggata
     1201 ctggctatag tgtaaccggc aaagagagga ccccaggctg cacttccagc atatctgctt
     1261 tgaaaaacta ccaagccaac ggttataggg aaaagttttg gttctgttac agttacaaga
     1321 ggccatatat attcgttcca tatattgagg aagttcatta gtccaacggt aaagaggata
     1381 gatttcgata gagggatggt tatctttaga aaacattgtg tctctgatgc cccatcaatc
     1441 ttggcagatt caaacagctc tttaggtatg gaagagaaga agcttctaag tataaatatc
     1501 ggcataacct gtcctccagc tatgtatggc aggataagtg cccagtaggt attcagcagt
     1561 ttgaggtctc tgaccaccag gaatctaggg acaagcgtca atatgcctgg aatcataagt
     1621 agagagagta tgaggaaaaa gaggatatct ttaccaggga agtcaaatcg ggtgaatata
     1681 taggcatcaa ttgaagataa gaccaggact ccaaacatgg taatcacact gacaattatg
     1741 gtgttcaaca aaaaaggaga gacctctttc caggcatcaa gataatttct atactccagg
     1801 gaagagggga gtccaaaaaa attatggaag acctcctctg ttgttttaaa agaggagatt
     1861 aacataaaga taaatggata tatacttata aaaaccagaa tgactatgac tatatgaaag
     1921 ggcaggtcat gtttaaactc agcaagtctc atcagccttc ctccgaagac cttatatatt
     1981 tcaggtttat ataagtcagt accaggagta tagaaaaaag tatcatccct atgctgcagg
     2041 cataccccat cttattaaag tagaatgcat tgttataaag gtacaaacct gggaccatgg
     2101 tactgttacc tggtccccca tttgtcagca ccattatacc tacgtatccc tggagagcat
     2161 ttatggtact gagtataagg attaacttga tctgtcccag tatcagaggt atatctataa
     2221 atcttatcct tttcaaccct gtaaccccat caatcttggc agcatcaaat atatctgttg
     2281 aaatgttctg taacccggcc aggaatatca aaaaccacag actggatatc caggggaaac
     2341 ccaaaaatag aatagcgtat agtgcggtgg aaaaatctcc taaccaagct ctgcagagat
     2401 tggagagtcc tatagccttt aaaaattgat tcaaaagtcc gtactgagga ttatagacaa
     2461 acttccatac cagcaagttt accatagatg gaacaaccat cggtataaca agcagtgtcc
     2521 tgtagatata tgccatcttt gtattaaaga tgccgaagat caattctgct gcgaataggg
     2581 gtacagtaat agctattatt acccgaaaca gggtaaggat agttatgttt ttggcagaga
     2641 tagccataac tgtatcttta aagattagct ggataaaatt ttctagcccc acgtatctgg
     2701 gaggagtaaa gccatcccat tcaaagaaag aataatacac tgctgagagt gcaggatagt
     2761 attcaaagac gaaaagtaga ataaatgtag ggagcagtaa taaataacaa tttctattct
     2821 taattatctc tctaaaaagt tttttcattg ttcccccagg gctgattatt tctttattcc
     2881 ccattccggg tgggtatcta tgagagcgtt ggctgccttt tccatctcta cctgaatctg
     2941 ctcgcatgcc tgctgtagtg ttagtttatc ggctaaatac tgctgtagta tctgatagaa
     3001 agcctcggca tactctctgg tgagacccct atagaatgat tctatcctga aaactcctct
     3061 ctccaaagat ttgacaaatg gctccaatct tttatctaat ccctttctcg cctttaatgc
     3121 cggagcagca gctccaaggt catttgccat agggatataa ttttgagggg cggttataaa
     3181 catcagccag tctatcgttt catcaaccag tcttttatta ctggttgtat atgggataat
     3241 ccagctacca gccccggtta ctcctccaac cgcagcggca gagattccag ttgcataggg
     3301 agagctttcc ttagtgattc ttggaaagta aaaaagtcca tattcgaact ttctcagagg
     3361 gtccacctct acaggtttta tctgtgcagc actctcatac atcatcgccg cctgcccatt
     3421 tataaagagc atataggcac cctgggagtc cactccaagg tatccatcct cccagtattt
     3481 ggaccattcc ttaaatagtc ttaactgttc ctgccattgc ggatccctgg cactatatac
     3541 tcctctcttt atagcttgaa ccagctcctt ctgacttata gcggaggctt ctatggcggt
     3601 tctttttatc ttccctttaa ctccttttat ctcagccatt ttggagtcca gaatcatatc
     3661 ctgtattgtc ctgaggctcc agttagctct catcttctct cccgccatag gaaatgcaaa
     3721 tggtatatag ccagcacgct ttatcttctc ctgtatgtcc ataaattcct tccatgtaga
     3781 tggtggtctt accccaacct tcttaaaaat gtccttgttg taaaagatag ctgttccggt
     3841 gatacctgtg ggaagaacat actgactctt tccgtcagga gccttacctg ttgctatggc
     3901 tatctccagg aagatatcct tccacttttt attatctttc acatatggat tgggtttttc
     3961 aagaaatgaa gttagatcta cataccatcc cttgtaagca tttctattga cctcagagaa
     4021 taattgttct gatatctctg gagctgtacc tcctgtcagc
//
LOCUS       DAOTKU010000105         4038 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_18833_length_4038_cov_4.113231, whole genome shotgun sequence.
ACCESSION   DAOTKU010000105 DAOTKU010000000
VERSION     DAOTKU010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4038
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18833_length_4038_cov_4.113231"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="P5310_03515"
     CDS             complement(<1..284)
                     /locus_tag="P5310_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32461.1"
                     /translation="MKVRSLTALILGIILFILGIVILVSSGSAGGVIPVLIGGALSYL
                     GWRGGRIALIVFGHTCIVLGCFLITWGIYLLPYSKPTLSHIFTRPLFWGL"
     gene            complement(532..1404)
                     /locus_tag="P5310_03520"
     CDS             complement(532..1404)
                     /locus_tag="P5310_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013414019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32462.1"
                     /translation="MSLTLFLKNKDVKDAFKKEFKIPRFKLQGELLAPPLTNHYALVG
                     TAFDYLLRFYIKRLNPNAIEQPWISEVSLDLLKEAQLDNLGLDNLFEMVEESPVYKKV
                     VEIIDYSKLVYGEFIKNGILNSDLFKAVLLLAQVDVIYRAGIVDENLGTVDKKDIEDL
                     RNLISIVKPEHFKAKKVCILNPTFGKASQLVGGADCDLVIDDTIIDIKTTKKLELTRS
                     YFDQLIGYYILYKIAGIDGMASSCEIKRLGIYFSRHAYFHSIDVRDIMKEDTFPNFVE
                     WFKERADEEYGYHI"
     gene            complement(1469..3202)
                     /locus_tag="P5310_03525"
     CDS             complement(1469..3202)
                     /locus_tag="P5310_03525"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HRU32463.1"
                     /translation="MDLKVTLADESYKAAYGIDSYGEDMVFEDSLNNFISCRGDWVAF
                     QVLIKLDEDFYLNVGRRANFSPKGPIRNLRVECNVEELPVEISPIGFIEDDDRVYKAD
                     LILNDEVIFVEKDKIQPVWVEISIPRDASSGEYNGRVCIYSQCMFDNEELIKTLDFTI
                     TVKDVTIPEPKDFRFYLDLWQHPSNIARKHEVHLWSGEHFKVLENYVKSLADLGQKAI
                     TVIASEIPWSGQRCYRVKNYPSDLFEYSMVRIERDSSGYHYDFSAVERYIDLCMRYGI
                     DEEIEVFGLINIWLDPEYGYTGIAEDYPDGIRLRYYDREDGCYKYIRSGNDIKAYIKA
                     LEQFFIRKGLIDKVRIVADEPGDIEKYRKSLETIREVADSFKFKAAINHPEFIDEFKD
                     TITDFVPVLPGVCEKWELLKELQKKINGRLLWYVCCWPPYPNTFISSPLVEAKLIGIL
                     TAFMGLDGFLRWNYTVWPENPRERISYRYPHWKAGDTNFVYPGRDGRPVLTLRYKSLK
                     RGIEDYELIMMLKDKSKEIPDELWNKIFKTDRIEEFHPNSGKGPSLLYSLDSKDYEEF
                     RRLVIEALESS"
     gene            3462..>4038
                     /locus_tag="P5310_03530"
     CDS             3462..>4038
                     /locus_tag="P5310_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HRU32464.1"
                     /translation="MESLRRGIRKNLVFFVLSILFSILALSLDMFNPRIVQEIIDGVI
                     IGGRMEDLSRLLLFLSIITLSRVLLGYGRGYFMDLGGSALASDIRVDLLKHLQTLPLS
                     FFDQINTGELMSRIKEDIDNVWFTFGFALMFFVEQVFYFIIAGIILFGINRELTLLIV
                     LLLPIIGYIAYKLEVRIDKVYGEISDQGVRLN"
BASE COUNT         1171 a          864 c          665 g         1338 t
ORIGIN      
        1 aataaacccc agaaaagggg acgcgtaaaa atatgcgata aagtaggttt ggaatatggc
       61 aaaagatata tcccccaagt gatcaaaaaa catcccagca caatgcaggt atgaccgaaa
      121 acaataagtg caatgcgacc acctctccat cccagataag acagtgcacc accgatgagc
      181 acagggatta ccccgccggc actaccacta cttacaagta taactatccc cagaataaat
      241 agaattatcc ctaagattaa agcagttagg ctacgaacct tcatcctttt ccccctgttc
      301 attttcgatt taatcataag catcttcaga tacccccaat actatataac tgataagtcc
      361 aacattaatc tgtctccgct ttttgccaat ggcagctaac ggctgcagac atacgaaggc
      421 gttcctgaaa gggccgattt ggatggagcg cagtgaagta ttttatccgc tgttagatgt
      481 cctgaaaggc aaggttgcca ggtaactgta gagtttagag attcagtttg attatatatg
      541 atatccatat tcttcatctg ccctttcttt aaaccattct acaaaatttg ggaacgtatc
      601 ttctttcatg atatctcgca catcaataga gtgaaaatat gcatgtctag agaaatatat
      661 tcccaatcgt tttatttcac aacttgatgc cataccatca atacccgcaa ttttgtagag
      721 gatataatag cctatcaatt gatcaaaata acttctcgtc aattcgagtt tttttgttgt
      781 ttttatatct attattgtat catctattac tagatcacaa tctgctcccc cgactaattg
      841 agaggccttt ccaaatgttg gattcaatat acatactttc tttgctttga agtgttctgg
      901 ttttactatt gatatcaaat tccttaagtc ttctatgtct tttttatcga cagtacctaa
      961 attttcatct acaattccag ctctataaat aacatccacc tgagccaaca acaatactgc
     1021 tttaaataaa tcagaattca ggattccatt tttaataaat tccccataaa caagttttga
     1081 atagtctatt atctctacaa cttttttata aaccggagat tcctctacca tctcgaacaa
     1141 attgtccaaa cctaaattgt ccaactgtgc ctctttcaat aaatcaagag aaacctcact
     1201 aatccaaggt tgctcgatgg catttggatt tagccgtttt atataaaaac gaagtaagta
     1261 atcaaatgct gtcccaacaa gtgcataatg atttgtcaaa ggaggtgcta ataattcgcc
     1321 ttggagttta aatcgtggta ttttaaattc ttttttgaaa gcgtctttta cgtctttatt
     1381 tttaaggaac aatgttaaac tcataatcca agcctccaaa tttcacttga ctcattcata
     1441 gacgaaattt tccccctggc attttacctt atgaactttc taatgcctct attacaagcc
     1501 ttctgaattc ctcatagtct ttactatcta aactatacag taggcttggt cccttgcctg
     1561 aattcggatg gaattcttct atcctgtcgg tcttaaatat cttattccac agctcatctg
     1621 gtatctcttt agatttatct ttcagcatca ttatcagctc gtaatcctct atccctcttt
     1681 taagactttt atacctgagc gtgagtacag gtcttccatc cctgccagga tatacaaagt
     1741 ttgtatctcc agccttccag tgggggtatc gataggagat tctctctctg ggattctctg
     1801 gccatacagt gtagttccat ctcaggaagc catcaagtcc cataaatgcg gtaagtatac
     1861 ctataagttt cgcttcaact aatggtgagc ttataaatgt atttggatag ggtggccagc
     1921 aacatacata ccacaataaa cgaccattta tcttcttttg aagttctttc aatagctccc
     1981 atttttcaca tacccctgga agtacaggta caaaatcggt tatcgtatcc ttgaactcat
     2041 ctataaactc aggatgattt atcgccgcct tgaatttaaa actgtccgca acctctctta
     2101 tcgtctccag actttttctg tatttctcta tgtctccagg ttcgtctgcc actatcctga
     2161 ccttatctat aagacccttt cttataaaga attgttccag tgcctttata tatgctttta
     2221 tatcatttcc acttcttata tacttataac atccatcctc tctgtcatag tatcttagcc
     2281 ttatcccatc aggataatct tctgcaatac cggtatatcc atattcaggg tcaagccata
     2341 tatttataag accaaagacc tcaatctctt cgtctatgcc gtatctcata cagaggtcta
     2401 tgtatctttc aactgcagag aaatcatagt gataacctga actatccctc tcaatcctca
     2461 ccatactata ttcaaataga tctgaggggt aattcttgac cctgtaacac ctctgtcctg
     2521 accagggaat ctctgatgct ataacagtta tagccttctg acctaggtct gcaagtgact
     2581 tgacgtagtt ttccagcact ttaaagtgtt cgccgctcca tagatgcacc tcatgtttac
     2641 gtgctatatt tgatggatgc tgccataaat ccagataaaa tctaaaatct ttgggctcag
     2701 ggatagttac atcttttaca gtaatggtaa aatcgagtgt ctttataagc tcctcattgt
     2761 caaacataca ttgagagtat atgcataccc tgccattata ctctccggaa gaggcatctc
     2821 tgggaataga aatttcaacc cagacaggtt gtatcttatc tttctctaca aagatcacct
     2881 catcgtttaa tatcaggtct gccttgtata ccctgtcatc gtcctctata aatccgatag
     2941 gagaaatttc caccggtaac tcctctacgt tgcattctac ccttagattt ctgatcggac
     3001 ccttcggaga aaaatttgct ctcctgccaa cgtttagata gaaatcttca tccagtttta
     3061 taagaacctg gaatgctacc cagtctcccc tgcaggagat aaaattattt aggctatctt
     3121 caaagaccat atcctcacca taagagtcta tcccataagc agccttatat gattcatctg
     3181 caagagtaac tttcagatcc attattaacc tcccactaat tcaatattaa aatctatttt
     3241 accacaaata tagaaagacg ttttgagaaa tgtcaatagt ctatgaaaat gtcaggggaa
     3301 ggaaatgaaa catatgagat taatttgtat ttcgtagggg caattcatga attgccccta
     3361 caatactgtc acctgtcacg gcatttatac gttaacaggg aattgacttt ttactgatta
     3421 ttctatacaa tactcttaca atctaaacag gggtgatgga cttggaaagc cttagaaggg
     3481 gtatcaggaa gaatcttgtc ttttttgttc tttccatttt attttctatc cttgctcttt
     3541 cactggatat gtttaatccg aggatagttc aggagattat agatggtgtt attattggag
     3601 ggagaatgga agatttatca aggcttctct tatttctctc tataataaca ttgtcccgtg
     3661 tattattggg atatggaaga gggtacttta tggatctggg cggttctgcg ctagccagtg
     3721 atataagggt tgatcttctc aaacatctgc agacccttcc tctatcgttc tttgatcaga
     3781 taaataccgg tgaattaatg tcaaggataa aagaggatat tgataatgtt tggtttacct
     3841 ttgggtttgc tcttatgttt tttgtggaac aggtgttcta ctttattatc gccggtatca
     3901 tattatttgg cattaacagg gaactgacct tattaattgt tctattgttg ccaattatag
     3961 gatatattgc atataaactg gaggtcagga tcgataaggt ctacggagag atctctgacc
     4021 agggggtaag gctgaata
//
LOCUS       DAOTKU010000106         3983 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_19153_length_3983_cov_3.066955, whole genome shotgun sequence.
ACCESSION   DAOTKU010000106 DAOTKU010000000
VERSION     DAOTKU010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3983
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19153_length_3983_cov_3.066955"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1491)
                     /locus_tag="P5310_03535"
     CDS             complement(<1..1491)
                     /locus_tag="P5310_03535"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HRU32465.1"
                     /translation="MEERWLKKKVEELLEKMTLEEKIAQLGSIPSGKLVENGRFSREK
                     AQELLKNGIGQITRVAGYAEREPEESVEIINEIQKFLKEETRLGIPAIIHEECLSGVM
                     TKGATTFPQAIGMASTFEPSYIEKMTSIIRKEMRAFGVHQGLSPVLDIPRDPRWGRTE
                     ETFGEDPYLVSKMAESYINGLQGDDWKDGIIATAKHFTAYGISEGGRNLGPARVSERE
                     LREVFLFPFEVAVRSAKVGSIMNAYHEIDGIPCASSRFLLTKILREEWDFKGFVVSDY
                     HAIDMLHTFHKVANSLKTAAIKALEAGIEIELPEIKCYGEPLFEAVREGLISMSVIDT
                     AVARVLRAKILLGLMDDNIYADPVKINSILDSPEHRMFARELARKSMVLLKNDSVLPL
                     KKDIKSIAIIGPSADVTKNLHGDYSYTAHIAGVKDGVKTVTVLEGIKNKVSSETTVYH
                     AAGCDLAGESREGFKEAVDVANKSEVIIAVMGENSGLFKRGLSGEGN"
     gene            complement(1503..2474)
                     /gene="pdxA"
                     /locus_tag="P5310_03540"
     CDS             complement(1503..2474)
                     /gene="pdxA"
                     /locus_tag="P5310_03540"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012011222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HRU32466.1"
                     /translation="MLIAITMGDANGVGPEILLKSFANGSIKESFIVIGDYSVLSFAS
                     EFLNYNIPVRKVDSTEDVKDGYLNVLDMEILDRDDIDIGKVSKKAGYASIMYVEKATR
                     LALEEKLNAIVTLPINKEAVRLTFPDFTGHTEFIANLCGDKDYSMMLASEKLIVTHLT
                     THIPLRSAIDMVKEENVYKLILLTYRTVERFIRNPKIAVAGLNPHAGEHSSFGREDEE
                     EIKPAVDRARRDGIDVEGPVPPDTVFWKAYNGSYNAVVCMYHDQGHIPVKLLDFEGGV
                     NITIGLKIVRTSVDHGTAYDIAYKGVASTRSLENAFRFAVRLATKNN"
     gene            complement(2574..>3983)
                     /locus_tag="P5310_03545"
     CDS             complement(2574..>3983)
                     /locus_tag="P5310_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32467.1"
                     /translation="LFDYNIGYLYNVKKITGKVEKDPQVLMAEGYFLEPSIRWEQRID
                     NGYPNVFYDEAYKEYRCYYTCFIKDQASTEFRLDQRINLEYPLHRYPPRTAGILMATS
                     KDGIHWIHPSFGICEYEGGRDNNILLTNVHGASVFRDEHETDPARRYKMAMRHDAHKK
                     VAVSFSTDGIHWCDPIDLPNYNAPGDTHNFAFWDDAIDRYVLITRLWAQGVIRVVARS
                     ESEDFINWSHPVEIYRGDGFDDQLYSMPVFKLRDVYYGLGSVYHGGDRASKDFDCVDC
                     ELLYSLDGLNWERAVRGESFIPRGMGRYGDDVPDCGCIYASIPVEQNGRYIFYYFGSN
                     GQHTNFRESSLMCASINPNKLAGYAAKSSEGGYLGTCQVKITGDRLIVTADISDGGSL
                     RAAVCRPGSLHSPINPIDGYQFENSIVEEHTDGEYHISFKTGKLSQLPEEPLIVVFHL
                     DKTVLYGYDGDIEPIRRKK"
BASE COUNT         1023 a          981 c          718 g         1261 t
ORIGIN      
        1 gttaccctct ccagataaac ctctcttgaa gagcccgcta ttttctccca tcaccgctat
       61 tatcacttca gatttatttg caacatccac agcctctttg aacccttccc ttgattctcc
      121 tgccaggtca caacctgcag cgtggtatac agtggtttca gaagatactt tattctttat
      181 cccttctaaa acagtaactg tttttacccc atcctttact ccagctatat gagccgtata
      241 gctataatct ccatgtaaat tctttgtgac atcggcactt ggacctataa ttgcgatact
      301 ctttatatcc tttttcagag ggagtacact atcgtttttc agtagcacca tagattttct
      361 ggctaattct ctggcgaaca tcctgtgttc agggctatca agtatagagt ttatcttaac
      421 tggatctgca tatatattat catccatcag acccaggagg attttagccc tcaagaccct
      481 tgctaccgct gtatctatta cagacattga tatgagacct tctctgaccg cttcaaaaag
      541 aggttctcca taacatttta tctctggtag ttctatctca attccagcct ccagggcttt
      601 tatagctgct gtttttaaac tatttgcaac cttatggaat gtgtgaagca tatcaatagc
      661 atgatagtct gagactacga atcctttaaa gtcccactcc tctctgagta ttttggttaa
      721 gagaaacctg gaggatgcgc aggggatacc atctatctca tggtacgcat tcattataga
      781 acccaccttt gctgatctta ccgcaacctc aaacgggaat aaaaagacct ctcttagctc
      841 tctctcagat actcttgccg gtccaagatt ccttccacct tcagatatac cgtacgctgt
      901 aaaatgtttg gcggtagcta ttatcccatc cttccagtca tcgccctgga gtccatttat
      961 atagctttca gccattttag acaccagata tgggtcttca ccaaatgttt cctctgtcct
     1021 tccccatctc gggtctcttg gtatatctag gacaggagac agaccttggt gaacaccaaa
     1081 tgccctcatc tcttttctta ttatggatgt catcttctct atatatgaag gttcaaatgt
     1141 gcttgccata cctatagcct gtggaaatgt agttgctcct tttgtcataa ctccagagag
     1201 acattcttca tgaattattg caggtatacc aagtcttgtt tcttccttca gaaatttctg
     1261 tatctcattt attatttcta ccgattcttc tggttctctc tctgcatatc ctgccactct
     1321 ggttatctgc cctataccat ttttaagtaa ctcctgcgcc ttttctctgg aaaaccttcc
     1381 gttttcaacc aatttaccgg atggtataga cccgagctgt gcaatctttt cttccagtgt
     1441 catcttctcg agtaactctt ctactttctt cttcagccat ctttcttcca tacgaaacct
     1501 cctcaattat tttttgttgc cagccttaca gcaaatctga aggcattctc aagacttctg
     1561 gtgcttgcta cgcctttata cgctatatca taggctgtac catggtcaac agatgttctc
     1621 actatcttta aacctatagt tatattcaca ccaccttcaa aatctagtag ttttacaggt
     1681 atgtgtccct gatcatggta catacagacc acggcattat atgaaccgtt atatgctttc
     1741 cagaataccg tgtctggagg cacaggtcct tccacatcta taccatccct tctggctcta
     1801 tcaactgcag gttttatctc ctcctcatcc tctctaccaa aagaggaatg ctcacccgca
     1861 tgggggttta atccagcaac agcaatcttt ggattcctga taaacctctc aaccgttctg
     1921 taggtaagta gtattaactt atagacgttt tcttctttta ccatatctat ggcagacctt
     1981 aaggggatat gggtggttag atgagtaact ataagttttt ctgaggcaag catcattgag
     2041 taatctttat caccgcagag atttgctata aactcggtat gcccggtaaa atccgggaag
     2101 gttaatctga cagcctcttt atttatgggg agtgtgacta tcgcatttag tttttcttca
     2161 agagccagcc tggtagcttt ttccacatac ataatggagg catatcctgc cttcttggag
     2221 acttttccga tatctatatc atctctgtcc agtatctcca tatcaagcac attcagatag
     2281 ccatctttaa catcttcagt ggagtccact tttcttacag ggatattgta attgagaaac
     2341 tcactggcaa aagagaggac actgtaatcg cctataacaa taaaactctc ttttatacta
     2401 ccattagcaa aacttttaag aagtatttcc ggtccaaccc catttgcatc acccatggta
     2461 atagctataa gcatcctgac acctcaaagt ttaagtttat aaaaaatatt ataacatctg
     2521 agggtcagaa attcttacct gtctctttta taaggggcat atacaatatg cccctacttc
     2581 tttctgcgaa taggctctat gtctccgtca taaccataaa gcactgtttt gtctagatgg
     2641 aatacaacta tcaacggctc ttccggaagc tgactgagtt tacctgtttt aaaactgata
     2701 tggtattctc cgtcagtatg ttcttcgacg atgctattct caaattgata accatcgatg
     2761 gggttgattg gactgtgcag tgaacctggt ctgcacaccg cagccctgag tgatccacca
     2821 tctgagatgt ccgctgtaac gatcagtcta tcgccggtaa tcttcacctg gcacgtaccg
     2881 aggtaaccac cctctgagga tttcgctgcg taacctgcaa gtttattggg attgatggat
     2941 gcgcacataa gggaactttc cctgaagttt gtgtgctgac cattgctgcc gaaataatag
     3001 aagatatatc ttccattctg ttctacaggt atacttgcat agatgcagcc acagtcaggg
     3061 acatcgtctc catatctacc catacctctg gggataaatg attcccctct tacagctcgc
     3121 tcccagttca aaccatccag actgtatagt aactcacagt ccacgcaatc aaagtctttg
     3181 ctggcacggt ctccaccatg atagaccgac cccaatccgt agtagacatc ccttaactta
     3241 aaaaccggca tagagtagag ctgatcatca aatccatcac cccgatagat ctctactggg
     3301 tgactccagt tgataaaatc ctcactttca gatctggcaa ctactcgaat tacaccctgt
     3361 gcccacaggc gagtaatcag tacataccgg tcgatagcat catcccagaa ggcgaaatta
     3421 tgggtatcac caggggcgtt gtaattgggg aggtcgatcg ggtcacacca gtgaatacca
     3481 tccgtagaaa agctaacagc cactttctta tgagcatcat gccgcattgc catcttgtac
     3541 cgtctggcag ggtcagtttc gtgctcatcc ctgaatacgg atgccccatg gacatttgta
     3601 aggaggatat tgttatccct gcctccctca tactcgcaga taccaaacga tggatggatc
     3661 cagtggattc cgtcctttga agtggccatc agtattcctg ctgtcctggg tggatatcga
     3721 tgaagaggat actccagatt tatccgttgg tcaagacgga actcagtgct ggcttgatct
     3781 ttgataaagc atgtatagta acagcggtat tctttatatg cctcatcgta gaatacgttt
     3841 gggtatccat tgtcgatacg ttgttcccag cgtattgaag gctctaaaaa gtagccttct
     3901 gccataagaa cttgcgggtc tttttcgact ttccctgtta tcttcttaac attgtacagg
     3961 tatcctatat tataatcaaa taa
//
LOCUS       DAOTKU010000107         3976 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_19187_length_3976_cov_3.726600, whole genome shotgun sequence.
ACCESSION   DAOTKU010000107 DAOTKU010000000
VERSION     DAOTKU010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3976
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19187_length_3976_cov_3.726600"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1541
                     /locus_tag="P5310_03550"
     CDS             <1..1541
                     /locus_tag="P5310_03550"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HRU32468.1"
                     /translation="DKLMDRPMAKLILVTATNPTPKGEGKTTTTIGLGDSLTKLGKKT
                     IICLREPSLGPVFGIKGGAVGGGKAKVFPESDINLHFTGDIHAISSAHNLLSAIIDNH
                     IYQGNDLGIDPLQIYWKRCVDMNDRQLRSIVSGLGGKSNGIPREDGFEITAASEVMAI
                     LSLSKDISDLKERLGSIIIGVNKEGKPVFSKNLKVEGAMSVLLKDAISPNLVQTLEGT
                     PAFIHGGPFGNIAHGCNSIIATKLASLLADYVVTEAGFGVDLGGEKFFDIKCRGGNFH
                     PATVVLVTSIRSLKFHGGVNQKELEIENVDVLDKGLPNLLHHIEVLKDVFKVPVVVAI
                     NKFPADTEGEITFLTEKLKGIGVRFAISNVYELGSDGGLELAREVLNSISSDKNLFEY
                     LYNLDAPYEEKIERIARMVYGADGVNFTDEAKIDLDRLYRWGIKNLPVCIAKTQYSLT
                     DNPKLLGRPRGFHINIQRLKASLGAGFIVAYAGSILTMPGLPKHPSAVKMDIDREGKI
                     TGIG"
     gene            complement(1538..2590)
                     /locus_tag="P5310_03555"
     CDS             complement(1538..2590)
                     /locus_tag="P5310_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRU32469.1"
                     /translation="MTSRERVRMTLEHREPDRVPIHDSVWAATVSRWHKEGLPEEIPV
                     SEYFDYEIVGFGADLSPRFPVKVLERNETYIVETTPYGGIRRNFRDYSTTPEIIDWPV
                     KSKEDWYKIKDRLQPNITRVNWVSSLNDYSRTYGDGKFVTYSAAMGYDQFQSYIKTEE
                     LLILLVEDPEWAKDMFRTHAILLIEMAKIMMEYGFKFDGAFLYNDMGYRNGLLFSPRT
                     YQEVLQETDAMVCEFFHKNNMPVILHSCGNVKELIPYLIDAGFDCLQPLEVKAGMDLL
                     ELKPEYGDKISFMGGIDVRKMTDPVAIEEEISRKFEVAKKGGGYIYHSDHSIPKNISF
                     EDYNRVISLVKKYGKY"
     gene            complement(2609..3436)
                     /locus_tag="P5310_03560"
     CDS             complement(2609..3436)
                     /locus_tag="P5310_03560"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRU32470.1"
                     /translation="MNTDKWEYVKICITALVAFSVVLSSYLYRSKIHIAGLEKDLTLA
                     YTKIQDLKRLEAINNKQRSEIVRLNSEAEKLKKKVEDVEKLTKQVQILTEKTFETSLS
                     SRRDASKYASRGGASLDEVYKKFNSLEKDIDNQKEQLVQVRSHLEILYERKLSVPAGY
                     PVSGYISSGFGWRRGSFHSGVDICVNRGNPVMATANGTVSYAGWKGGYGLTVIIRHNF
                     GFSTLYAHLSKVSVNAGKRVARGDVIGYSGMTGYATGPHLHYEVMVNDKPVDPRNYL"
     gene            complement(3449..3823)
                     /locus_tag="P5310_03565"
     CDS             complement(3449..3823)
                     /locus_tag="P5310_03565"
                     /inference="COORDINATES: protein motif:HMM:NF025935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4446 family protein"
                     /protein_id="HRU32471.1"
                     /translation="MMDNTVLFIIGAIIILNIVLLIILLILRSNLKKIEEKQKGELIL
                     SSFSIGGTKYDAFQDTSGKLSFSLAFVAGDEKGIVLTSIHTREQSFLYLKPYNKDKSN
                     LTEEEKRAIEVALGEGYTVKEL"
BASE COUNT         1244 a          824 c          713 g         1195 t
ORIGIN      
        1 ttgataagtt aatggataga ccgatggcaa agttaattct tgttaccgct actaacccta
       61 ctccaaaagg ggaaggaaag actacaacca ctattgggtt gggggattcc cttacaaaac
      121 tgggtaaaaa gaccattatt tgcttaagag aaccatctct tggtcctgta ttcggtatta
      181 agggtggagc tgttggagga ggaaaggcta aagtattccc tgagtcagat ataaatcttc
      241 actttacagg ggatatacat gcaatatctt ctgcacacaa cttattatca gctataatag
      301 ataatcatat ataccagggt aatgacctgg ggatagaccc tctccagata tactggaaaa
      361 gatgtgttga tatgaatgat agacagttaa gatctatagt gagtggattg ggaggaaaga
      421 gtaacggaat acccagagaa gacgggtttg agataacagc tgcatcagaa gttatggcta
      481 tattatcatt gtcaaaagac atttcagatt taaaagaacg tcttggcagt ataattattg
      541 gggtaaataa agagggaaaa cctgtgttta gtaaaaatct gaaggtagag ggggcgatgt
      601 cagttcttct aaaagatgca atctcgccaa atctagttca gactctggag ggaactccag
      661 cattcatcca tggcggacca tttggtaata tagcacacgg atgtaattct ataatagcaa
      721 ctaaactggc cagcctgctg gcggattatg tggtcacaga ggcaggattt ggtgtagact
      781 tagggggaga aaagttcttc gatataaaat gcaggggagg taatttccat ccagctacgg
      841 tggtacttgt aacatcgata aggtcattaa aattccatgg aggagtaaat caaaaagaac
      901 tggagataga aaatgtagat gtattagata aaggtctacc aaatctatta caccatatag
      961 aggtgctgaa ggatgtattt aaagtaccgg tagttgtcgc cataaacaaa tttccggcag
     1021 atactgaggg agagataact tttctaacag aaaaacttaa gggtattggt gtaagatttg
     1081 ccatatccaa tgtgtatgaa cttggatctg atggtggatt agagctggct agagaggtac
     1141 taaattctat ctctagcgat aaaaatttat tcgaatatct atataactta gatgcccctt
     1201 acgaagagaa gatagaaagg atagcaagaa tggtctatgg tgcagatggg gtaaatttta
     1261 ccgatgaggc aaaaatagac cttgacaggc tatacagatg gggtatcaaa aatcttcccg
     1321 tttgtatagc aaaaacacag tactccttaa cagacaatcc aaaactttta ggtagaccca
     1381 gaggttttca tataaacatt caaagactaa aagcatcact gggagcagga tttatagttg
     1441 cctacgcagg ctcaattctc acaatgccag ggctacccaa acatccttca gcggtaaaga
     1501 tggacatcga tagagaggga aaaattacag gtataggcta atacttacca tatttcttca
     1561 ccagacttat aacacgattg taatcttcga aacttatatt tttagggata gagtggtcgc
     1621 tatgataaat atatcctcct cccttctttg ccacctcaaa ttttctgctt atctcctctt
     1681 ctatagctac agggtcagtc atcttacgga catctattcc acccataaaa gaaatcttat
     1741 ccccatactc aggttttaat tccagaaggt ccattccagc cttaacctcc aatggctgga
     1801 ggcagtcaaa ccctgcatca ataagataag ggattaactc tttcacattc ccacatgaat
     1861 gcaatatcac aggcatatta tttttatgga aaaactcaca taccatagca tcagtctctt
     1921 gaagaacctc ctggtacgtt cttggcgaga ataagagtcc attcctgtat cccatatcat
     1981 tataaaggaa cgccccatca aacttgaaac catattccat cataatttta gccatctcta
     2041 taaggagtat ggcatgggtc ctaaacatat ctttagccca ttccggatct tctacaagaa
     2101 gaataagtaa ttcctctgtt tttatgtagg actgaaattg atcataaccc atagctgcac
     2161 tgtaggttac aaatttacca tctccataag tcctgctgta gtcgtttaaa gaagaaaccc
     2221 aattaactct ggttatatta ggctgaagcc tgtcctttat cttataccag tcctctttgg
     2281 actttacagg ccaatctatt atctctggtg tagtagaata gtctctaaaa tttcttctta
     2341 tccctccata aggagtcgtc tcaactatat aggtctcatt cctctctaaa acctttaccg
     2401 gaaatctcgg agaaaggtct gctccaaatc caactatctc ataatcaaag tactcagaga
     2461 caggtatctc ctccggtaat ccttctttat gccatctact aacagtagca gcccatacac
     2521 tatcatgtat gggaacccta tctggctccc tatgttctaa ggtcatcctc actctctctc
     2581 tgctggtcat ttaaatctcc ctcctatttt ataaataatt cctcggatct acaggcttat
     2641 cgtttaccat cacctcataa tgaagatgag gtccagtagc atatccggtc attccagaat
     2701 agccaataac atcacctctt gcaaccctct tacctgcatt aactgatact tttgacagat
     2761 gagcgtataa ggtggagaag ccaaagttat gccttatgat tacagtaagt ccatagccac
     2821 ccttccaccc tgcatatgaa actgttccat ttgcagtagc cattacagga tttcctctat
     2881 tcacacatat atctacaccg ctatgaaaac ttccccttct ccatccaaaa ccagaagata
     2941 tataacctga tacaggataa ccagcaggga cagaaagctt tcgctcatac agaatttcta
     3001 agtgtgacct tacttgaact aattgttcct tttgattatc tatatctttt tctaaagagt
     3061 taaatttttt ataaacctca tctaaagatg caccgcctct tgatgcatat ttagatgcgt
     3121 ctcttctgga agacaggcta gtttcaaaag tcttctctgt caggatttga acttgtttag
     3181 tgagtttctc cacgtcttca acttttttct tcagtttttc tgcctcagaa tttaatctga
     3241 ctatttcaga tcgctgttta ttatttatcg cctctaacct ctttaaatcc tgaatcttcg
     3301 tataggcaag ggtaaggtct ttttcaagac cagcgatatg tattttggat ctatataaat
     3361 aggaagataa tactacagaa aaagcaacta aagcagttat acaaatcttc acatattccc
     3421 acttatctgt attcaatatt aaccctcctt atagttcctt tactgtataa ccttctccca
     3481 gggctacctc tatcgctctt ttctcttcct cggtaaggtt actcttatcc ttattgtaag
     3541 gcttaagata aaggaaagac tgttctcttg tatgaataga tgtaagaact atacctttct
     3601 catcacctgc tacgaaagcc aaagaaaaac tgagtttgcc tgatgtatcc tgaaatgcat
     3661 catatttagt cccaccgata gaaaaagaag agagtatcag ctcccctttc tgcttttctt
     3721 ctatcttctt taggttggac cttaaaataa gcagaataat aagaagaaca atatttaata
     3781 ttatgatagc tccgataata aagaggacgg tattatccat catagagctt tctcctcttt
     3841 taatagaacc aagcctggct ttataaagtg tccttgcccc tcttttccaa agactactat
     3901 gctaccacct ctttttaata aaggaaaaac catcggtttc agataatcca tatcactgac
     3961 agccctggaa agaacc
//
LOCUS       DAOTKU010000108         3968 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_19228_length_3968_cov_2.749808, whole genome shotgun sequence.
ACCESSION   DAOTKU010000108 DAOTKU010000000
VERSION     DAOTKU010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3968
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19228_length_3968_cov_2.749808"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..854
                     /locus_tag="P5310_03570"
     CDS             <1..854
                     /locus_tag="P5310_03570"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRU32472.1"
                     /translation="SLSASDYSNLEGLTRYIEESLVPSHISVSIPSLRIDTVSGDFYK
                     AIGEIKRGLTFAPEAGTQRLRDVINKNITEDDIFKTVELVSREGWKAVKLYFMIGLPT
                     ETEEDILGIADLLNNIKKMYKKIELNVSVSVFVPKPHTPFQWAGQITPEEAKKKITLI
                     RKNLVRRDINMSWHDPRVSLLEGILSRGGAELGDIIIKSWRKGVRFDAWTDQFNWDIW
                     DSSFIEEGIDFIGYLKEKTFLDPLPWDFVDAGVRKEFLWDEYISGLSGVRREDCRKAG
                     CYNCGVC"
     gene            844..1431
                     /locus_tag="P5310_03575"
     CDS             844..1431
                     /locus_tag="P5310_03575"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HRU32473.1"
                     /translation="MFARVEYKKYGPLVFISHLDLIRCWDRITRRSGIPFSFTEGFHP
                     RPRFSFAPPLPVGFEGERELLDIALESPYSSIKEAINKVVPYGIYVNNVYFIEHSDLG
                     DSIIGGLYNFQWMGMVKEEDLGEGLKSSGIKDWIYSSEPLNQDLYGFSGTKNSLSIPI
                     LFVKGSPSPKRIVELIDGFGENIWLKLSRSLLLEK"
     gene            1470..2354
                     /locus_tag="P5310_03580"
     CDS             1470..2354
                     /locus_tag="P5310_03580"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRU32474.1"
                     /translation="MGTLPFASYRDNRKDFLSLVSYACSLGINLFDTAEIYDNYELLG
                     RALKGFEDVFVITKSYAITREDVDRSLLRARRLMQRDADIFLLHEQESSLTLKGHMEA
                     LEYLNKLKENGDLRATGISTHRVGGVKGALEYSDLIDIIMTILNFSGLGITDGNRDDM
                     EMAIKDCFSFDIGTIGMKILGGGHLLRDINRAFSYVRRLDFIHSFLIGVEDREELEMD
                     IRLINGLISGDTISVVKGKERYIEIEPWCTGCGLCVDRCSQGAISIVNCKAEVDKEKC
                     VLCSYCAQVCPDFSIKVV"
     gene            2351..2785
                     /gene="ruvX"
                     /locus_tag="P5310_03585"
     CDS             2351..2785
                     /gene="ruvX"
                     /locus_tag="P5310_03585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HRU32475.1"
                     /translation="MRYLGIDFGLKRIGLAISDESNKIAFGLNTLENRGPKDFFNNIK
                     LLINKYNISKIVIGFPTNMDGTPGNLSRELSKFIDTLKKIPEVEIITWDERLTSVEGG
                     KIARELGKKDKGTIDRIAATLILQSYLDYINRLTTPEGPSTL"
     gene            complement(2757..3635)
                     /locus_tag="P5310_03590"
     CDS             complement(2757..3635)
                     /locus_tag="P5310_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009223885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRU32476.1"
                     /translation="MDKFSGNERYLIINADDLGMCHSSNEAIFKLFEDGFISSSTLMT
                     PCPWVKEVAEYYRSHPDIDIGVHLTFTSEWKYYKWGPVTKNKIVDSLITEEGYFPERS
                     EDVELRANREDVREEIKNQIDLAVKLGINPSHLDNHMGSLYGLTFGNSFLDIIFEFCQ
                     RYNFPFRLPKILDEEMKQGLPEAAISNYEKMIDIADQNGIKLIDHLISYSFSLEDGED
                     YTSFKERVISILRNLKPGINELYIHPGLNTEELKAINPHWEKRYMEFKLYQDEKLWKV
                     LQEEGIKLIRWKDLRG"
     gene            complement(3640..>3968)
                     /gene="nadE"
                     /locus_tag="P5310_03595"
     CDS             complement(3640..>3968)
                     /gene="nadE"
                     /locus_tag="P5310_03595"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013931348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HRU32477.1"
                     /translation="VRYGDDASDIAPISHLYKTEVMEFAKFLNLPEKIIEKEPTPDLI
                     PGITDEFSIGISYGDLDRILKAFEEGKTPEEIEGVDKDMLNLIYDQYMWIKKQEKKPL
                     HLERRV"
BASE COUNT         1210 a          714 c          845 g         1199 t
ORIGIN      
        1 tatctctgag cgccagtgac tatagtaatt tagagggctt gaccagatat atagaggaat
       61 cattagtccc ctcacacata tctgtatcga taccttctct aaggatagat actgtgtcag
      121 gtgacttcta caaggctata ggcgaaataa aaagaggact tacatttgcc ccggaggctg
      181 ggactcaaag gttgagggat gtaataaata aaaatataac cgaggatgat atatttaaga
      241 ctgtagaact tgtttccaga gaaggatgga aagctgtgaa gttatacttt atgataggac
      301 tccccactga gacggaagag gatattcttg gtatagctga ccttctcaat aatataaaga
      361 aaatgtataa gaaaatagaa ttaaacgtga gtgtctctgt gtttgttccc aagccccata
      421 cccctttcca gtgggcaggg cagataaccc cggaagaagc gaagaagaag attacactta
      481 taaggaagaa tttagtcaga agagatatta atatgagctg gcatgacccc agagtaagtc
      541 ttcttgaggg tatactatca agaggtggtg cagaacttgg agatataatt ataaagtcat
      601 ggagaaaagg tgtaagattt gatgcctgga cagaccagtt taactgggat atatgggata
      661 gttcctttat agaagaggga attgatttta taggttatct gaaagagaag acttttctgg
      721 acccattgcc atgggatttt gttgatgcgg gagtaaggaa agagtttctc tgggatgagt
      781 atatcagtgg actttccggt gtaagaagag aggactgcag aaaggcagga tgttataact
      841 gtggtgtttg ctagggttga gtataaaaaa tatggacctc tggtatttat ctctcatctg
      901 gaccttatac gttgctggga caggataact agaagatcag gaattccatt cagttttact
      961 gagggattcc atcccagacc cagattctca tttgccccac ctcttccggt aggatttgag
     1021 ggagaaagag aactactgga tatagcactg gaatctcctt attccagtat aaaagaggct
     1081 ataaataagg tggttccata tgggatatac gtaaataatg tatactttat tgaacattca
     1141 gacctggggg attcaatcat aggaggatta tacaacttcc agtggatggg aatggtaaaa
     1201 gaagaagatc tgggtgaggg attgaaaagt tctggtatta aagactggat atactcttca
     1261 gagcctctta atcaagatct gtatggattt tccggtacaa agaattccct ctctattccc
     1321 attctttttg taaaggggtc tccttcacca aagagaatag tggagttaat agatggtttt
     1381 ggcgagaata tatggttaaa actctctagg agcctcttat tagaaaagta atactgggta
     1441 gatctaatat tccggtctca aatatagcaa ttggcacatt gccatttgca tcttacaggg
     1501 ataacaggaa agatttttta tcccttgtct cttacgcctg cagtttggga ataaacctct
     1561 ttgataccgc tgagatttat gataactatg aacttctggg gagagccctg aaggggtttg
     1621 aagatgtctt tgtcatcacc aaatcttatg ccataaccag agaggatgtt gataggagtc
     1681 ttttgagagc cagaagatta atgcaaagag atgcagatat atttcttctt cacgagcagg
     1741 aatcttccct tactcttaag ggtcatatgg aagcgctgga gtatttgaat aagttaaagg
     1801 aaaatggtga cctcagagca accgggatat caacccacag ggttggcggt gtaaaggggg
     1861 ctttagaata ttcagattta atagatataa ttatgactat tttgaatttc tccgggcttg
     1921 gcataactga tgggaataga gatgatatgg aaatggcgat taaagattgt ttctctttcg
     1981 atatcgggac tatagggatg aaaattttgg gagggggaca tctcctgaga gatataaaca
     2041 gggcattttc ctatgtgaga aggctggact tcatacattc tttccttata ggggtggaag
     2101 atagagaaga attggagatg gacatacgtc taattaatgg attaatatca ggcgatacaa
     2161 ttagtgtagt aaaggggaaa gagagataca tagagataga gccctggtgt accgggtgcg
     2221 gtctatgtgt ggacagatgt tctcagggag cgataagtat agtgaattgt aaagctgaag
     2281 tggataagga gaaatgcgtt ctatgcagtt attgtgctca ggtatgtccc gatttctcta
     2341 taaaggtagt atgagatacc tgggaataga ctttggttta aagagaatag gtctggctat
     2401 aagtgatgag agtaataaga tagcctttgg tctgaataca cttgagaata gaggaccaaa
     2461 ggactttttt aacaatatta aacttttaat caataagtat aatatatcca aaatagtaat
     2521 cggttttccc accaatatgg atggtacacc tggaaatctc tccagagaac tgtctaaatt
     2581 tatagatacc cttaaaaaga tacctgaagt ggaaataatt acctgggatg aacgattaac
     2641 ttcggttgag gggggaaaaa tagcaagaga actcgggaag aaagataagg gcacaataga
     2701 caggattgca gctacgttga tcctgcagag ttatcttgac tatataaatc gtttaactac
     2761 ccccgaaggt ccttccacct tataagtttt atcccctctt cctgtaagac cttccacagc
     2821 ttttcatcct ggtatagttt aaattccata tagcgttttt cccagtgggg atttatagcc
     2881 ttcagctctt cagtatttag ccctggatga atataaagct catttatccc tggctttaga
     2941 tttctcaaaa tactgatcac tctctcctta aaagatgtgt aatcctcccc atcttcaaga
     3001 gaaaaagaat aagaaatcag atggtctatt aattttatcc cattttgatc tgctatgtcg
     3061 atcatctttt catagttaga aatagctgct tcaggtaacc cctgcttcat ctcctcatcc
     3121 aagatttttg gtaatctgaa tgggaaatta tacctctgac agaactcaaa tattatatct
     3181 aggaagctat tcccaaacgt cagcccatag agactcccca tatgattatc cagatgggag
     3241 ggatttatcc caagttttac tgcaaggtct atctgattct taatctcttc tctcacatct
     3301 tccctgttag ctctgagttc tacatcttcg gacctctccg ggaaataccc ctcttcagtt
     3361 atcagagaat ccacaatttt attctttgtt accggtcccc acttataata cttccattca
     3421 cttgtgaatg ttaagtgcac tccgatatct atatctggat gagaacggta atactcagcc
     3481 acctctttga cccatggaca gggagtcatc aaagtactag aagatataaa tccatcttca
     3541 aacaacttaa aaattgcctc atttgaggaa tgacacatcc caaggtcatc cgcattgatt
     3601 atcagatatc tctcatttcc ggaaaactta tccataatct tatactctcc tttccaaatg
     3661 aagaggtttc ttttcctgtt ttttaatcca catatactgg tcatatatta aattcagcat
     3721 atctttatcc acaccctcta tctcctctgg agtttttcct tcttcaaaag ccttcagtat
     3781 cctgtccaga tctccatacg atatacctat ggagaattca tcagtgatac caggtatcag
     3841 gtctggtgtt ggctctttct ctattatctt ctccggtaga ttcaggaact tggcaaattc
     3901 cattacctct gtcttataca agtgacttat tggagctata tcagaggcat catctccata
     3961 tctgacat
//
LOCUS       DAOTKU010000109         3932 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_19445_length_3932_cov_3.309776, whole genome shotgun sequence.
ACCESSION   DAOTKU010000109 DAOTKU010000000
VERSION     DAOTKU010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3932
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19445_length_3932_cov_3.309776"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..768)
                     /gene="gltX"
                     /locus_tag="P5310_03600"
     CDS             complement(<1..768)
                     /gene="gltX"
                     /locus_tag="P5310_03600"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HRU32478.1"
                     /translation="MGVRVRFAPSPSGSLHIGNVRTALFNWLFARKNNGIFILRIEDT
                     DIERTYESAYSGILEDLAWLGLNWDEGPYKQSDRLDIYKSYAERFLKEGLAYKCFCSP
                     EELEREREQALKEGKPPRYSGKCANLTGEEISRLESERRSCSIRFRIPEDACFVVEDI
                     TRGKIEFDVNAITGDFIILRSDGMPTYNFAVVIDDHLMKITHVIRGEDHLSNTPRQLL
                     IYRALEAEPPVFAHLPMIVGSDHSKLSKREGSFSVKEL"
     gene            complement(759..1238)
                     /gene="ispF"
                     /locus_tag="P5310_03605"
     CDS             complement(759..1238)
                     /gene="ispF"
                     /locus_tag="P5310_03605"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012474060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HRU32479.1"
                     /translation="MRVGIGYDVHPIVRGRRLFLGGIEIPCEFGLGGHSDADVLLHAI
                     SDALLGAAGLSDIGTFFPNTPAYKDISSCVILEGVRKLIKGYTIINIDSTVITEKPKL
                     SPYISSMREKISAILEINNNSIGIKATTNEKLGFIGREEGIAAIAVVLIDERRIQWV"
     gene            complement(1235..1915)
                     /gene="ispD"
                     /locus_tag="P5310_03610"
     CDS             complement(1235..1915)
                     /gene="ispD"
                     /locus_tag="P5310_03610"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:TIGR00453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HRU32480.1"
                     /translation="MKLGVIIASAGQSKRLGHNKVLMELGSKPVLLYSLEKFLAIKTL
                     SELVIVIRAQDEMDVRDILSRAGYSHSKIKVVQGGKERQDSVHIGLKSLVDSDIVAVH
                     DGARPFISLDLILKLIKEAEDNPAVIPALPVKETIKTVKNGYVESTPDREFLWSVQTP
                     QVFKYEILINAYERAYKDNFYGTDDSSLVERLGYKVKVVEGEYTNMKITTKEDLLLAR
                     RYLELLGL"
     gene            complement(1912..2997)
                     /gene="disA"
                     /locus_tag="P5310_03615"
     CDS             complement(1912..2997)
                     /gene="disA"
                     /locus_tag="P5310_03615"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018978394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning diadenylate cyclase DisA"
                     /protein_id="HRU32481.1"
                     /translation="MLDLSRSLKLTAPGTLLREGLDRILSAKMGALIVVGGNLEQLNS
                     IISGGFSLNCDFTPEAVYELAKMDGAIVISEDLKKILYANVQLSPDISISTVEPGTRH
                     KTAERVAKQISSLVIAISERREIITLYIGNTRYVLQDIRNLLVKANQGLQVLERYRLI
                     LDQAMSNLTTLELEDSVTLFDILWSLYRIEMVVRIAQEVERYIIELGIEGRLVKMQLD
                     ELIGGVQEEEILLIKDYIGEGNSPEAVRDEIRKKNLNEILNANAIASLLNITDFTGPT
                     DTNLRCKGYRILSYISRLPSSIIENLVSHFGCLQNILKADIPQLDEVEGIGEIRAKNI
                     KDGLRRLREQGNRGIPPFRLGDMGRTR"
     gene            complement(2981..>3932)
                     /locus_tag="P5310_03620"
     CDS             complement(2981..>3932)
                     /locus_tag="P5310_03620"
                     /inference="COORDINATES: protein motif:HMM:NF024931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HRU32482.1"
                     /translation="EGKNLMIISESSLDSAISLYEEMKPDLVIIDSIQAVRDESLEAQ
                     PGTVSQIKLCTSRIIDIAKRDNSVVFITGHVTKEGMLAGPKTLEHMVDGVFYFEGERD
                     GMIRLLRSVKNRYGDTNEVAFMEMTSEGLKEINNPSLFFIREGLGTTPGATIGVVMEG
                     TRPILVEVQALVTRSVYTMPKRVVQGIDFNRFSFIIAVLEKLIQLTLFNKDIFLNIPG
                     GFNIKDPAIDLPVALAIISSTIEIPLSSEIFSFGEIGLTGEIRQVMWYEKRIETALRL
                     GYSEGIAPLQRVTHSKMKGVRTLREVYELIKNARSGVRSI"
BASE COUNT         1041 a          923 c          617 g         1351 t
ORIGIN      
        1 gagttcttta acagaaaaag aaccttctct tttggataac ttggaatggt cgcttcccac
       61 tatcataggt agatgggcaa acactggggg ttctgcttct aaagccctgt atatcagtaa
      121 ttgccttgga gtattcgaca gatggtcttc tccccttatc acatgtgtaa tcttcataag
      181 atgatcgtct ataactactg caaaattata ggttggcatc ccatctgaac gcagtatgat
      241 aaaatctcct gttatagcat tcacatcgaa ttctatttta cctctggtaa tatcttctac
      301 tacaaaacag gcgtcttctg gtatcctgaa ccttatagag caggaccttc gctcagattc
      361 taacctggat atctcctctc cggttagatt tgcacacttt ccactgtaac ggggaggttt
      421 tccctctttt aaagcctgtt ctctttctct ctccagttct tctggtgaac aaaaacactt
      481 atacgccaat ccttctttta aaaatctttc ggcataactt ttatatatgt ctaatctatc
      541 actctgttta tatggtccct catcccaatt cagtcctaac caggcgaggt cttctaggat
      601 accagaatag gctgattcat aagtcctctc tatgtcggta tcttctatcc ggagtatgaa
      661 tattccgtta ttcttccttg caaatagcca gttaaatagg gcagttctta cattacctat
      721 atgcaagctt ccactgggac ttggggcaaa tcttactctt acacccattg gattctcctt
      781 tcgtcaatca atacaactgc aatagccgct ataccctctt ctcttccaat aaatccgagt
      841 ttttcatttg ttgttgcctt tatgccgata ctgttattgt ttatttccag tatagcagat
      901 atcttttctc tcatcgaact gatatatgga ctgagcttcg gtttttctgt aataacagta
      961 ctgtctatat ttattatagt atagcctttt atcagttttc ttacaccttc cagtataaca
     1021 cagctagata tatctttata tgcaggagta tttgggaaga atgtaccgat atctgacagt
     1081 ccagctgctc ctaacagcgc atctgagatg gcatggagca atacatctgc atctgaatgt
     1141 cctccgagac caaattcaca gggtatttct atacccccaa gaaagagtct tcttcctcta
     1201 actataggat ggacatcgta ccctatacca actctcatag acctaacaat tcaagatacc
     1261 tgcgagctaa aagaaggtct tctttggtgg taattttcat attagtatat tctccctcaa
     1321 ccactttaac cttatatcct agtctttcta ccagtgatga atcatctgtt ccatagaaat
     1381 tatctttata cgccctttca taagcattta tcaggatttc gtatttaaag acctgtgggg
     1441 tctgaacact ccataagaat tccctgtcgg gagtagactc aacataacca ttttttactg
     1501 tttttatcgt ctcttttaca ggtaaggcag gaattacagc aggattgtcc tctgcctctt
     1561 ttattagttt caggataagg tcaagactta taaatggtct tgccccatca tgaacagcta
     1621 ctatatcaga atctactaga gacttaagac ctatatgaac agaatcctgt ctttccttac
     1681 caccctggac cacctttatt ttagaatggc tataccctgc cctggaaagt atatctctaa
     1741 catccatctc gtcttgagcc cttataacga taaccagttc tgatagagtc tttatggcga
     1801 gaaacttttc cagagaatat aaaagtacag gtttgctacc cagctccatc aataccttat
     1861 tatgtccaag ccttttagat tgaccagcag aagctattat tactcctaat ttcacctagt
     1921 ccttcccata tctcctaatc taaatggtgg aattcctctg tttccctgct ccctgagtct
     1981 tcttaatcca tcttttatat tcttagctct tatctctcct attccttcta cctcatccag
     2041 ttgtgggatg tctgctttta gtatattctg cagacatcca aaatgactga caaggttttc
     2101 tataatagaa gatggtagtc gagagatata actcaagatt ctatacccct tacatctcag
     2161 gtttgtatca gttggacctg taaagtcggt tatatttagt agacttgcta tagcatttgc
     2221 atttaatatc tcatttaagt ttttctttct tatctcatct ctgacagcct caggactatt
     2281 cccttcacct atataatctt ttatgagtaa aatttcctcc tcctgaaccc cacctataag
     2341 ttcatcaagt tgcatcttta ctaatcttcc ctcaatgccg agttctataa tatatctttc
     2401 tacctcctgg gctatacgca cgaccatctc aattcgatat aaactccata atatatcaaa
     2461 cagtgtgaca ctatcttcca gttccagtgt ggtaagattt gacatagcct gatctaatat
     2521 tagcctgtat ctttccagca cctggagccc ctgattagcc tttaccagta gattcctgat
     2581 atcctgcaag acgtatcgtg tatttcctat atacagcgtt attatttctc ttctttctga
     2641 gatggcaata accaatgaac ttatctgttt tgctacccgt tccgctgttt tatgtcttgt
     2701 ccccggctct actgttgata tagatatatc tggagaaagc tggacattcg catagagtat
     2761 cttctttaaa tcttctgaga ttacaatagc cccatccatt tttgccagtt cgtatacagc
     2821 ttccggagta aaatcacagt ttaagctaaa tcccccactt ataatactgt tgagttgttc
     2881 caggttacct ccgacaacta taagagctcc catctttgca ctcagtatcc tgtctaagcc
     2941 ctctctcagc agggttcctg gagcagtaag ttttaatgat ctagatagat ctaacaccgg
     3001 atctggcatt ttttattaat tcatacacct cccttaatgt ccttactcct ttcattttag
     3061 agtgagtcac tctttgtaat ggagcgatac cttcgctata tcccaatctc agtgctgttt
     3121 ctattctttt ttcgtaccac ataacctgtc ttatttcacc tgtaagtcct atctctccaa
     3181 agctgaagat ctcagaagaa aggggaatct ctatagtgct agatattatt gctaaagcca
     3241 caggcagatc tatggccggg tcctttatat taaaaccacc agggatattc agaaatatat
     3301 ctttgttgaa gagagttaat tggatgagct tttctaatac cgctattata aatgaaaatc
     3361 tattaaaatc tataccctgt accacccttt taggcattgt atatacagaa cgtgtgacta
     3421 atgcctgaac ttctacaagt atcggtcttg ttccctccat tactaccccg atagtggcac
     3481 ccggagtagt gcccaagcct tctctgataa agaatagaga tggattattt atctctttaa
     3541 gcccttcgga agtcatctcc ataaatgcaa cttcgtttgt atctccgtat ctattcttta
     3601 ccgaacggag gagtcttatc attccatctc tctctccttc aaagtagaag actccatcaa
     3661 ccatatgttc taatgtctta ggacctgcta acatcccctc tttggttaca tgtcctgtta
     3721 tgaagaccac actattatct cttttggcta tatctataat tcttgatgtg cagagtttta
     3781 tctgacttac agttccaggc tgagcttcca gagactcatc ccttacagcc tggatagaat
     3841 ctataataac gaggtcaggt ttcatctcct catagaggct tatagctgaa tcaaggctac
     3901 tttcacttat tatcataaga tttttgccct ct
//
LOCUS       DAOTKU010000110         3924 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_19499_length_3924_cov_3.546911, whole genome shotgun sequence.
ACCESSION   DAOTKU010000110 DAOTKU010000000
VERSION     DAOTKU010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3924
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19499_length_3924_cov_3.546911"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1171)
                     /locus_tag="P5310_03625"
     CDS             complement(<1..1171)
                     /locus_tag="P5310_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRU32483.1"
                     /translation="MRIKSIRTRTLVYILPLTIAIMIALSALSFISFRGVIDEEMDDK
                     LKYQIGQVAEMTNKLLIQHSRIPEVLARFVESMGNKLTKEEYISILKKIVTTNADTFG
                     TGIWFEPYRYSKDVKYFGPYVYKEGDEIKVTLEYEEEWYDYPSWPWYKMAVNTKKSIE
                     WSEPFYDETTGVTMITASAPFYDQEGKLLGLTTGDIDLAHLQDMIAGMKVGNTGYVFL
                     LSKDGQYIASPDKELVMKRRITDSENKSLADLGMQIIKNREGIGYYTEGDEKIRVCYT
                     LLPETGWFVCAAIPESELFAPVQGLLIRLLLIMLISILAIVYAVFHYSSYISRNILKV
                     NEFSKKIALGDLSSGLSLKSEDELGEMAGNLNTMAESMRSIIKNILNSTDQLLQTG"
     gene            1424..2725
                     /locus_tag="P5310_03630"
     CDS             1424..2725
                     /locus_tag="P5310_03630"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HRU32484.1"
                     /translation="MVNLKELFKYPDKNYRSIPFWSWNDRLDPDELDRQIREMKSQGM
                     GGFFMHSRDGLETPYLGEEWMTCIRRAVQTAKEAGINAWLYDEDRWPSGFAGGLVAKE
                     IGDEGRAKALTLEAVRGEIDPDRSLASFVVVLENNFISRLKRIKEKTRLADNELGLIF
                     KREICRKSERGDAYGDNLNPKSVEAFIKTTYERYLKEVGEEFGKTIPGIFTDEPNIFA
                     WRGDKRALPWTDILPEFFRSRRGYNILEELPYLFFDGEKSCRIRYDYWRTISELFLES
                     YTKQICRWCQEHNLLFTGHYLYENEFYRGILRTGSFMPYYIYQGVPGIDILSEFITET
                     LTVKQCSSVANQFGKKYVLSELYGCTGWEFTFEGQKWVGEWQYALGVNLRCQHLALYT
                     LRGCRKRDYPPSFNYNTTRMATDCVLTVNLLPSLFMCMQIF"
     gene            2707..2850
                     /locus_tag="P5310_03635"
     CDS             2707..2850
                     /locus_tag="P5310_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32485.1"
                     /translation="MHANLLTTIDMMKLVTDYWKNLGIKAEVKPETNVLVGAKVQANE
                     FEM"
     gene            3046..3225
                     /locus_tag="P5310_03640"
     CDS             3046..3225
                     /locus_tag="P5310_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32486.1"
                     /translation="MLKEAMRLHSDNLWIIGTVGVSWMMAIVKNNLRNVPESGFTWHP
                     AAPGVFRLEQFFFKK"
     gene            3358..3810
                     /locus_tag="P5310_03645"
     CDS             3358..3810
                     /locus_tag="P5310_03645"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HRU32487.1"
                     /translation="MLSSSCMEDDNREELEPVNDFKYIPIYRKIPFDLPKEEWKNYIV
                     DYLPIPYTPENAFALIVPDNAMSNDDIIEGSTVVVIESLDFDRFNGRVVALKLKDSEV
                     TLRRLYFLNSEVILAASNPDYDTFIVSDDDLENINIIGLVSYLVKNMG"
BASE COUNT         1183 a          735 c          826 g         1180 t
ORIGIN      
        1 gtccagtttg cagcaattga tccgtactgt ttaaaatatt tttaattatg cttctcatgg
       61 actcagccat agtatttaga ttccctgcca tctcacccag ctcatcttca ctttttaaag
      121 agagtccaga tgatagatcc ccgagggcaa tctttttgga aaactcattt accttcagga
      181 tatttctgct tatgtagcta ctatagtgga agacggcata gacaattgcc agtatggaga
      241 tgagcattat cagcaaaagt cttataagca gcccctgaac aggtgcaaaa agttcacttt
      301 ctggaatagc tgcacacaca aaccatcccg tctcaggcag cagagtatag cacacccgta
      361 ttttctcatc accctcggtg tagtatccta tgccttcacg attttttata atttgcatac
      421 ctaaatctgc taacgattta ttctcgctgt ccgttatcct tctcttcatc accaactctt
      481 tatcaggaga ggctatgtac tgtccgtcct tgctcaataa gaaaacatat ccagtattgc
      541 cgaccttcat acctgctatc atatcctgta ggtgagccag atcaatgtca cctgtggtaa
      601 gcccaagcaa tttcccttcc tggtcataaa aaggagcact tgctgttatc atcgtaacgc
      661 cggtggtttc gtcataaaaa ggctcagacc attctatact ttttttcgta tttacagcca
      721 tcttatacca gggccagctt gggtagtcat accattcttc ttcatattcc agggtaacct
      781 ttatttcatc cccttctttg tatacatagg gtccaaaata cttgacatct ttactatagc
      841 ggtagggctc aaaccatata cctgttccaa aagtgtcggc atttgtggta actatctttt
      901 tcaagataga tatatactct tcttttgtaa gcttattccc catcgattct acaaaacgag
      961 ccaatacttc gggtattcta ctatgctgta tcagcagttt atttgtcatt tctgcaactt
     1021 gtcctatctg atacttcagt ttatcgtcca tttcttcatc aataacaccc ctaaaagaga
     1081 taaagcttaa agcagataaa gctatcatga tagctatcgt caggggcaaa atatatacca
     1141 gagttctcgt tcgaatactt ttaatacgca tcttttataa cctcctcttt acagtattta
     1201 atatccctta tggagtcagt atctaagcct cttagatttg gctctccggt aatacttcct
     1261 tattatacaa aaaaatctca taatgattaa ctctattcct gaagggatat ataccgtaaa
     1321 ggataaaacg tcaattcatt ctcagcagtc attcataaat attcaattat acggctatat
     1381 gttagaatga tggttggata taaaagcgtt ctggaggtta ataatggtta atttaaagga
     1441 attattcaag tatccagata agaattacag gagtatccct ttctggtcat ggaatgatag
     1501 gcttgaccct gacgaattag ataggcagat aagggagatg aaatcccagg ggatgggagg
     1561 attcttcatg cactcaaggg atggtcttga gactccatac cttggtgaag aatggatgac
     1621 ctgtatcaga agggctgttc agaccgcaaa agaagcagga ataaatgcat ggctatacga
     1681 tgaggatcga tggccatcag gttttgccgg cggattggta gctaaagaga taggagatga
     1741 gggtagagct aaggctttaa ccttagaagc ggtaagggga gaaatagatc ctgacagaag
     1801 cctcgcaagt tttgtggttg tactggagaa taactttata tccaggttaa aaaggattaa
     1861 agaaaagacc cgtcttgctg ataatgagct tggtttaata tttaaaagag agatctgccg
     1921 gaagtctgaa aggggagatg catatggtga taatctgaat ccaaagagtg tagaggcatt
     1981 tataaagaca acctacgaga gatatttaaa agaggttgga gaagaattcg ggaagaccat
     2041 acctggaata tttactgatg aaccgaatat tttcgcttgg agaggtgata aacgagcttt
     2101 accctggaca gatatattac ctgaattttt caggtcaaga agaggctata atattcttga
     2161 agaacttccc tatctgtttt ttgatggaga aaaatcttgc aggataagat atgactactg
     2221 gaggacaatt tctgagttat ttcttgaatc ctatacaaaa cagatttgta gatggtgtca
     2281 agaacataat cttctcttta ctgggcacta tctgtatgag aatgaatttt acagaggaat
     2341 cctcagaacc ggttccttta tgccatatta tatatatcag ggggttcccg gtatagatat
     2401 cctgagcgag tttatcaccg agactctaac ggtaaaacag tgcagcagtg ttgccaatca
     2461 gtttggcaag aaatatgtac tttcagaatt gtatgggtgt actggatggg aatttacatt
     2521 tgagggacag aagtgggttg gtgagtggca gtatgcattg ggggtaaacc tcagatgcca
     2581 gcatctagcc ctctataccc tcaggggttg cagaaagaga gattatcctc catcatttaa
     2641 ttacaataca acaaggatgg ctacagattg cgtcctgacg gtaaacctct taccctcact
     2701 atttatgtgc atgcaaatct tttaaccacc atagatatga tgaaactggt tactgattac
     2761 tggaaaaatc tcggcataaa ggctgaagta aagccagaaa caaacgttct tgttggagca
     2821 aaagtccagg cgaacgaatt tgagatgtag ggatttacta tcagtgttgg tgctgatgga
     2881 tactctggtg atcttacacc caataacaac tggtgtccgc tatggtacag atggctgagt
     2941 actaatggga aatcaggaga agagccacct aaagagataa agcgactctg gcagatattt
     3001 aacgatgaat tttactccac caccagtgag gctaagagaa ccgctatgtt gaaagaagct
     3061 atgagacttc actcagataa cctctggatc ataggaacag ttggggtctc atggatgatg
     3121 gctatagtga agaataacct caggaatgta ccggagtcag gttttacgtg gcatcctgct
     3181 gctcctggag tcttcagatt agaacagttc ttcttcaaaa aataaattcc agcttgttaa
     3241 agccctggca attgtcaggg cttttttatt tcttctcaga atctagaggt aaatggagtc
     3301 ctagaaggtt tgctggaacc tgcgggaatt ttttggcttg tcaaatcctg ggttcgtatg
     3361 ttatcatctt cttgtatgga agatgataat agagaagagt tagaacctgt taatgatttt
     3421 aaatatatcc cgatctatag aaaaattccc tttgacctcc ccaaggaaga gtggaaaaat
     3481 tatatagttg actatcttcc tatcccatat acgcctgaga atgcgttcgc ccttatagtc
     3541 cctgataacg ccatgagcaa tgatgatatt atagagggaa gcactgttgt ggtgatagag
     3601 agcttggact ttgacagatt taatggcaga gtggtggctc ttaaacttaa agattcagag
     3661 gtaactttaa gaaggctcta ttttctcaat tcagaagtca ttcttgctgc ttcaaatccc
     3721 gattatgata catttatagt atctgacgat gacctggaaa atataaacat tattggtttg
     3781 gtgtcatatc tggttaagaa tatgggataa cttctggtag ccttttaaag gctatcagta
     3841 attcatcaag gttatcagga ggtgaaaaga tgatctgtaa gaatatccct gtatgggaag
     3901 gaaatcagga gatccatatg gaga
//
LOCUS       DAOTKU010000111         3878 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_19775_length_3878_cov_4.355218, whole genome shotgun sequence.
ACCESSION   DAOTKU010000111 DAOTKU010000000
VERSION     DAOTKU010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19775_length_3878_cov_4.355218"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..754)
                     /locus_tag="P5310_03650"
     CDS             complement(<1..754)
                     /locus_tag="P5310_03650"
                     /inference="COORDINATES: protein motif:HMM:NF012669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HRU32488.1"
                     /translation="MSITVIREKRKEQIVDFIRKCGIVSIKELAESLNLSAATIRKDV
                     RELEENGLVSRHWGGVSLKEGQVKELSFDEKTIINLVEKKAIAQRAITLVAQNDTIGI
                     SAGTTAYEFARLLDQYPNVQVVTYGLNIAYLIARMGGKVVVPGGFLRDNSYALVGEDT
                     VSFFKKIFLDKCFMGVDGLDIEAGLTDVNMAEAEVNRAMIESSGQLIIIADHTKLGQR
                     KLVPIAPIDRIDVLITDSLADKGILKILEDKGI"
     gene            918..3464
                     /locus_tag="P5310_03655"
     CDS             918..3464
                     /locus_tag="P5310_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32489.1"
                     /translation="MEKIIQPGGTIGQWLALGPIPWGRGEDIVEDSSKILTSSHHKGE
                     IFDSPSGKNYWEPVCRDEKYIAFGSDYESLLLWNKGYIPRRYAAVFLSNVIIPERAGA
                     TGFKVVTFTDIRAIIAVNGSIIYDKEISRDDNLYFEDQIEVMLNGGENTFNVCLLDAG
                     KAGHLGIMLELIDNIVEVTIPGNPESSRELEEQVEKEIKSLRLEKDLFYPEDRIKLHS
                     DTGISCKLSLLSRSGESILEKDLINEKDINLCYGKELEDGNYKIICIWKDDRGNYIAS
                     TSFNIIKLTPANPIKGAENLETREKLTLEYFASNPVWGRDEIWAQVARYKLDLDVDEE
                     IIKRACEYIKIRRDCSDFIMQAILRLMYWEKEKPRLSPRIRELMKEAILGFRYWIDEP
                     GERTMYMDTENHRFLFHTAEWLAGILFPTKEFTNSQQNGLYHSLKGRMYLAEWLKERT
                     RFGFDEWHSNSYYPVVFAGLANIYDFAPKEEYKIKIMAKHILDYIFFILAQDTFHGVF
                     GTTHGRCYGTRIKYPDCDESSSLCWLLYGEGNLCGGGMAGVSVATSTYRIPELVLDIA
                     SDQNTIVESYERQGLISYRDLSANLVVYKTPDYMISSVQDFQKGEYLDLIREAEILKP
                     GVAMYWSFPYTQMHVAQVTLENKVVIFWSCPVTSEEGTSLRPDYWAGNIVLPRAIQYR
                     NVLALIWKQGKFSWMTHCLFEKDRFDEVYMDDRWAFARVKDGYVGIYSQNGFNPGERG
                     QYTGRELICYAGENIWLAECGRKEDWGSFSNFIESIKRADIESVDGGLVYNSPSAGRF
                     LVGWDIEPSVNGEKIKIKDYPLLESPYGYSAFGSGHITLRFKDQIEEIFS"
     gene            complement(3504..>3878)
                     /locus_tag="P5310_03660"
     CDS             complement(3504..>3878)
                     /locus_tag="P5310_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRU32490.1"
                     /translation="RNVAVLSDPGRLLGTTTDGPWAPLYALWYTSGGKSGEEPKGDIR
                     KIYQFWDKVKVTADEKIRDRYFKEIINLHKRNIWIIGLVGELPQPAIAKNNLRNVPDG
                     IIWDDSVRSPKNARPEQFFFKK"
BASE COUNT         1211 a          733 c          833 g         1101 t
ORIGIN      
        1 caattccttt atcttcaagg atctttaaaa tccccttgtc tgccaggcta tctgtaatta
       61 acacatctat cctatctatg ggagctatgg gaactaattt tcgctgtccc aattttgtat
      121 ggtcggctat aattattaac tgcccggatg actctatcat cgccctgtta acctctgctt
      181 ctgccatatt gacatctgta agtccagcct ctatatcaag cccatccaca cccataaaac
      241 atttatccag aaatatcttc ttaaagaaac ttaccgtgtc ttctccaacc agtgcatagg
      301 aattatctct caagaaacct cctgggacaa caacctttcc ccccatcctg gctatcaggt
      361 atgctatatt taaaccatat gtaactacct gtacatttgg gtattgatcg agaagccttg
      421 caaattcata tgctgtagtc cctgcactta taccgatggt atcattctga gctaccaggg
      481 ttatagccct ttgagctata gccttcttct cgactagatt aattatagtc ttttcatcaa
      541 acgataattc ctttacctgt ccttctttta aagatactcc accccaatgt ctgctgacta
      601 gcccattttc ttccaattct cttacatcct tacgaatagt agcagcagat aaatttaaac
      661 tctcggctag ctctttgatt gagactatgc cgcattttct aataaaatcg actatttgtt
      721 cttttcgttt ctctcttata actgtaatag acatttatca atttttaatc actttcatct
      781 catattttaa ttaaattaaa tcatattatt tcaaatatgt caacaataac atcttctgag
      841 gttgtaagct ttaaaaagat atccattatg ctaaaatcaa tcctgatcac ctaattctaa
      901 ttaacaggag ggaaaaagtg gaaaagataa ttcagcctgg aggaacgata ggacagtggt
      961 tagccctggg accaattccc tggggaagag gagaagatat tgtagaagac tccagtaaga
     1021 tcttaacctc cagccatcat aaaggagaga tctttgattc tccatcaggt aaaaactact
     1081 gggagccagt ttgcagagat gagaaatata tagcttttgg cagtgattac gagagccttc
     1141 tattatggaa taaaggatat atacctaggc gttatgcagc ggtatttttg agtaacgtga
     1201 taatcccgga aagagcaggg gctacagggt ttaaagtcgt cacatttact gatatacggg
     1261 caataatagc tgtaaatggc tccatcatat atgataagga gatctccaga gatgacaatc
     1321 tatactttga agatcagatt gaagtgatgt taaacggggg tgagaataca tttaatgtct
     1381 gtcttttaga cgctggaaaa gctggacacc ttgggattat gctggagtta attgacaata
     1441 ttgttgaggt gaccatccct ggtaacccgg agtcttcccg tgaactggaa gaacaggtag
     1501 aaaaagagat aaaaagtctg aggttggaga aagatctctt ctatcctgaa gacaggataa
     1561 agctacattc agatacaggt atctcctgca aattatccct gctttcaagg agcggagaaa
     1621 gtatattaga aaaggactta ataaatgaaa aagacataaa cctgtgttat ggtaaagagt
     1681 tagaggatgg aaactacaag atcatctgca tatggaaaga cgatagggga aactatatag
     1741 ccagcaccag ctttaatatc attaagctca cacctgctaa tccaataaag ggtgcggaga
     1801 atcttgagac cagagagaaa ctcactctgg aatattttgc atccaatcct gtttggggtc
     1861 gtgatgagat atgggctcag gtggcaaggt acaaactgga cttagatgtg gatgaagaga
     1921 taataaagag agcctgtgag tatataaaga taagaagaga ctgctctgat ttcatcatgc
     1981 aggctatcct gaggcttatg tactgggaaa aagagaagcc gaggttgagt cctaggataa
     2041 gggaattaat gaaagaagca atcctgggct tcagatactg gatagacgaa cctggagaga
     2101 ggaccatgta tatggatact gaaaatcacc ggttcctctt ccatacagca gaatggctgg
     2161 caggaatact cttcccaact aaagaattta caaacagtca gcagaacggg ttatatcact
     2221 ctctgaaggg gaggatgtat ctggcagaat ggttaaaaga gagaaccaga ttcggatttg
     2281 atgaatggca ttccaacagc tattatccgg tagtctttgc agggttggca aatatatatg
     2341 attttgcacc taaagaggag tacaaaataa agataatggc aaaacatatt cttgattata
     2401 tattctttat acttgcccag gacacatttc atggggtatt tggaacaaca catggacgat
     2461 gctacgggac gaggataaaa tatccagact gcgatgagtc ttcctccctc tgctggttgt
     2521 tatatgggga agggaatctc tgtggtggag gaatggcagg ggtatcagta gctacaagta
     2581 cgtacaggat accggaatta gtccttgata ttgccagtga ccagaataca atagtagagt
     2641 catatgaaag acagggactg atatcgtaca gagatttatc agctaatctt gttgtatata
     2701 aaactccaga ttatatgatt tcatctgtcc aggattttca aaagggggag tatctggatt
     2761 tgatcagaga ggcagagata ttgaaacccg gagttgccat gtactggtct tttccctata
     2821 ctcagatgca tgttgcccag gtgacattag agaataaagt ggtaatattc tggtcctgcc
     2881 cagtgacatc agaagaggga acaagtttaa gacctgatta ctgggcaggg aatatagtgt
     2941 taccgagggc tatccagtac aggaatgtgc ttgcactgat atggaaacag gggaaattct
     3001 cctggatgac acactgtctc tttgaaaaag atagatttga cgaggtatat atggatgata
     3061 gatgggcttt tgcaagggtt aaagatggat atgtgggaat ctactctcag aatggtttta
     3121 atcctggaga gagaggacag tatacaggaa gagagctgat atgctatgca ggggaaaata
     3181 tatggctggc agaatgtgga agaaaagagg actggggctc atttagtaac tttatagaga
     3241 gtatcaaaag ggcagatata gaatctgtag atggaggact tgtatataac tctccatctg
     3301 caggaagatt tctggtaggc tgggatatag aaccttcggt aaacggtgaa aagattaaaa
     3361 taaaagatta tccactgctg gaaagcccat acggatattc agcctttgga agcggacata
     3421 taactctcag gtttaaagat cagatagaag aaatcttctc ttagaatatt aaggggcagg
     3481 agagattcct gcccccagat aagttacttc ttaaagaaga actgctctgg acgagcattc
     3541 ttaggagatc tcacactatc atcccaaata ataccgtcag ggacattcct gaggttattc
     3601 tttgctatcg ccggctgagg cagttctcct acaagcccta ttatccagat atttctttta
     3661 tgcagattta taatctcctt aaaatacctg tcccttatct tttcatcagc ggtaaccttt
     3721 accttatccc agaattgata tatcttcctt atatcaccct ttggctcctc accgctctta
     3781 ccaccactgg tataccaaag tgcatacagt ggagcccagg gaccatcagt tgttgttccg
     3841 agaagtcttc ccggatcaga taatacagca acattcct
//
LOCUS       DAOTKU010000112         3855 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_19926_length_3855_cov_4.543421, whole genome shotgun sequence.
ACCESSION   DAOTKU010000112 DAOTKU010000000
VERSION     DAOTKU010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3855
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19926_length_3855_cov_4.543421"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            97..1119
                     /locus_tag="P5310_03665"
     CDS             97..1119
                     /locus_tag="P5310_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011916667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRU32491.1"
                     /translation="MKYRRLGRTNIEVFPVAFGGIPIQRVGMDEAVKVIRRAIELGIN
                     LIDTARAYSDSEIKIREALKGVDKRVYISSKSQNRTRDGVLEDVKISLKNLGVEKIDI
                     YQLHGVNDRDAFERVLAPDGAYYGLLDAKEKGLIDFIAVSSHSTDILEELIRTDKFDV
                     IQLCYNFIETKVEERVFPMALEKDIGIIGMKPIGGGVMPNPALSLKYVLQKEFLVPDP
                     GMEAIKEVEENVRVAMELTEITSEEYEEIERIRKELGSTFCRRCEYCQPCPQGIPITS
                     IMRAKDFMKRLPKENLRNGWFYEAYLKAQNCTKCETCLPRCPYKLPIPDLIEENVKLI
                     KEFFGE"
     gene            1122..1604
                     /locus_tag="P5310_03670"
     CDS             1122..1604
                     /locus_tag="P5310_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HRU32492.1"
                     /translation="MPNVLIAYFTWSGNTRRIAELIHSKVDGTLVEIQPEVSYPRSYN
                     VVVNQAKREISMSHKPPLKTKIEHIESYDIIFVGSPNWWGTIAPPVATFLLGYDLRGK
                     TIVPFSTHGGGGQGRISVDIAKLCPGSTILKGFSIYGGELGRAEAMISAWLREIGILM
                     "
     gene            1615..1776
                     /locus_tag="P5310_03675"
     CDS             1615..1776
                     /locus_tag="P5310_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32493.1"
                     /translation="MDRVERSKEKLKQLIGDATYDSIRNAPQDQKHIQEYLSAHFVLV
                     TFTHVVDLI"
     gene            1865..2245
                     /locus_tag="P5310_03680"
     CDS             1865..2245
                     /locus_tag="P5310_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008399232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRU32494.1"
                     /translation="MTISEVSKKCEISADTLRYYERIGLIPKIYRNGNGIREYTEEDC
                     RWIEFVKCMRGAGIQVEALIEYIKLFQEGDSTIEARKKILIEQRDHLIDNIEKMQKAL
                     ERLNFKIKRYETRILPVENSLKPF"
     gene            2627..2941
                     /locus_tag="P5310_03685"
     CDS             2627..2941
                     /locus_tag="P5310_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32495.1"
                     /translation="MKTEEILNQIQGLEREKERIVTEARNKAQEIIETAKKEAAENIN
                     AARLNIEEERKLAIDKTRKEIEKLKEQMVRENALCIDKFNALIKERYQDLISEIKEKV
                     IG"
     gene            2942..>3855
                     /locus_tag="P5310_03690"
     CDS             2942..>3855
                     /locus_tag="P5310_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32496.1"
                     /translation="MAILPMLRLHIVGHKSEEEDVLEVLQELGVLEVERINLENSLPL
                     DFLPSKLSDLSKELSRIKTDINFLKGYVNTGGSIIDLFVPQKLSLSGDERDKSIREFN
                     DNKGYETIEAWQSSLEEIKTSINNLMQDRELLHPFESLNFSIRDIKGFKLTDVFFCQM
                     RNADFQKFTEEIEVLPAVVSRVLQLPQYTFFIVASHREVAGEIRGIFSRYNVTTFNLD
                     KFYGIPREELSGINSRIEGLKRKEEEILREIGKNGDKLLKSLYVIYDIYQNDYTRYEH
                     LNKVVHTDSMFLITGWIREKDKKTVEETL"
BASE COUNT         1339 a          557 c          915 g         1044 t
ORIGIN      
        1 gagtcacatt cctcagtttg tcaggtattg acttggagtt aactccaggt tatatactct
       61 tatagagtat ataatatcta taaggagtga taaaaggtga agtatagaag attgggaaga
      121 acaaacatag aagttttccc tgtagcattt ggcgggattc ctatccagag agtaggtatg
      181 gatgaggcgg taaaagtaat aagaagagct atagaactgg gaataaatct tatagatact
      241 gcaagagcct atagcgatag cgagataaag attagagagg cattgaaggg tgtagataag
      301 agggtatata tctcttcaaa gtcacaaaac aggacaagag atggagtact ggaagatgta
      361 aagattagtc taaagaattt aggggtagag aagatagata tataccagct ccatggggta
      421 aatgatagag atgcttttga gagagtgctt gctcctgatg gggcttatta tggacttctt
      481 gatgcaaaag agaaagggtt gattgatttt attgctgttt ccagccatag taccgatatt
      541 ctggaagaac ttatcaggac tgacaaattt gacgtaatcc agctctgtta taattttata
      601 gaaaccaaag tggaagaaag agtattccct atggctctgg agaaagatat tggaattatt
      661 ggcatgaagc caataggggg aggagtaatg ccaaatccag ccctctctct aaagtatgtc
      721 cttcagaagg aatttctggt tcctgatcca gggatggaag ctataaaaga ggtggaggaa
      781 aatgtaagag ttgcgatgga gcttacagaa attacctctg aggagtatga agagatagaa
      841 aggataagaa aagagcttgg ttctactttc tgcaggagat gcgaatactg tcagccgtgc
      901 cctcagggga ttcccataac atcaatcatg agagcaaaag actttatgaa gaggcttccc
      961 aaagaaaatc tgagaaatgg gtggttctat gaagcatacc ttaaagctca aaattgtaca
     1021 aaatgtgaga cttgtcttcc tagatgtcca tataagctac ctattccgga tttaatagaa
     1081 gagaatgtaa aacttataaa ggaatttttt ggagaataga catgccaaat gttcttattg
     1141 cgtattttac ctggtccgga aatacacgca ggattgcaga gttaatccac agtaaagttg
     1201 atgggactct ggtagaaatc cagcctgaag tatcttatcc caggtcgtac aatgtggttg
     1261 taaaccaggc aaaaagagaa atatctatga gtcataagcc tccattaaaa acaaagatcg
     1321 agcacataga atcatacgat ataattttcg ttggttcacc aaactggtgg ggtacaatag
     1381 ctcctcctgt tgccacattt ttattaggat acgatttgag aggaaagact attgtacctt
     1441 tctccacaca tggtggaggt ggacagggaa gaataagcgt tgatattgca aaactatgtc
     1501 ctggctcaac catcctgaaa ggattttcga tatatggagg tgagctggga agggcagaag
     1561 ctatgatatc tgcgtggctg cgtgagatag gaattcttat gtaaggaggc tgatatggat
     1621 agagttgaaa gaagtaaaga gaaactcaaa cagcttattg gtgatgcaac ttatgacagc
     1681 ataaggaatg cacctcagga tcaaaagcat atacaggaat atctctctgc tcattttgtt
     1741 ttggtgactt ttacacacgt ggtggacttg atttaaaaac acgggagtta ctaattttga
     1801 cacttaacgc tctttcctgt atcaacgaag taattccaga acaataagag gaggtgcaat
     1861 tgctgtgacc atctcagaag taagcaaaaa atgtgagatt tcagcagata ctctccgtta
     1921 ctatgagcgt attggattga ttccaaagat atatcgcaat ggaaatggca ttcgggagta
     1981 tactgaggaa gactgcagat ggattgaatt tgttaaatgt atgcgtggtg caggtatcca
     2041 ggttgaagcg ctgatcgaat atataaagtt attccaggag ggggattcaa ccatcgaagc
     2101 aaggaagaag attctgattg aacaacgaga ccatctcatt gataatattg agaagatgca
     2161 gaaggcactg gaaagactta acttcaaaat taaacggtat gagacgagaa ttctgcctgt
     2221 agaaaacagt ctgaagccat tttaatgtta aaaatataaa ctttggtact gaatttttcc
     2281 tgtaaaaggc tggttttctt tacactgaaa gattcctgag ataaattaat agatgaaact
     2341 taagtttttc caggaggtag cagatggaaa atgaaagatc atagagtcca atctagtttg
     2401 actaatcaaa gctgctatgg tagaatattt tcctgagtgg aagagatggt tggtcaatca
     2461 ctgccgcctg tagaggcagg aattctgatc agctgtcctc gccgcagtat ggttatatta
     2521 gcctgatcac agcactgtaa ggtattattg gatattatgt tagaaaggag gaagagcaca
     2581 tcatctccat cttagaggtg gagcctcctg tgcttgatat aacaatatga aaacagaaga
     2641 gattctgaat cagatacagg gtctggagag agaaaaggaa aggatagtaa ctgaagccag
     2701 gaataaggct caggagatta tagaaacagc caaaaaagag gcggctgaaa atataaatgc
     2761 agccaggtta aatatagagg aagagagaaa gttagctata gacaagacca ggaaagagat
     2821 agaaaaatta aaagagcaga tggtaagaga aaatgcctta tgtatagata aattcaatgc
     2881 gctgataaaa gagaggtatc aggacttaat atcagagata aaagaaaagg tgatcggata
     2941 aatggctatt ctgccaatgc ttagactcca tattgttggt cataagagtg aggaagaaga
     3001 tgtattggag gtattacagg agctgggagt tttagaggta gagagaataa accttgagaa
     3061 cagtttaccg ttagatttct tgccttcaaa gctttctgac ctcagtaaag aactctcgag
     3121 aataaaaaca gatataaatt tcctgaaagg atacgtaaac actggcggaa gtattataga
     3181 tttatttgtc cctcagaaat taagcttatc aggtgatgaa agggataaat ccataagaga
     3241 atttaatgat aataagggct atgagactat agaagcctgg cagagttctc tggaagagat
     3301 taaaacttca ataaataatt taatgcagga tagagaatta ttacatccat ttgaaagttt
     3361 gaacttctcc ataagagata taaaaggctt taaattgaca gatgtattct tctgtcagat
     3421 gagaaatgca gattttcaga aatttactga agagatagaa gtgttgccag ctgttgtatc
     3481 tagggtttta cagttgccgc agtatacatt ttttattgtt gcctctcacc gggaagtggc
     3541 tggtgagata agaggtatct tctccaggta taatgtaacc acttttaacc ttgataaatt
     3601 ctatgggata cctagagaag agctgtctgg gataaattcc aggatagaag ggttaaagag
     3661 gaaagaggaa gagattctaa gagagatagg taaaaatggg gataaactcc ttaaatcttt
     3721 gtacgttatc tatgatatat atcagaatga ttatactagg tatgaacatc ttaataaggt
     3781 ggtccatacc gatagtatgt ttctgataac aggttggatc agggagaaag ataaaaaaac
     3841 tgtagaggaa acact
//
LOCUS       DAOTKU010000113         3852 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_19942_length_3852_cov_3.161180, whole genome shotgun sequence.
ACCESSION   DAOTKU010000113 DAOTKU010000000
VERSION     DAOTKU010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3852
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19942_length_3852_cov_3.161180"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..737
                     /locus_tag="P5310_03695"
     CDS             <1..737
                     /locus_tag="P5310_03695"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRU32497.1"
                     /translation="LGMGDPAYKLLPQSMWEDKNLRSLYEYNPAMANQLLDELGLKKD
                     KSGYRVRPDGKPLTLTILVNLAYPIHPDVAEIVQKQWEAVGIKVAIDSISGALWWPRI
                     NTSEYQIVAYELDYTTDRYFLTYSRSFIPNANSTYWAPLWGLWYASDGKAEGSEKPTG
                     DALKLIALFDKIKVTTEEKQKAKLTADAVSLCAKDLFAIPTIAGYPQPCIVKNNFKNV
                     PKNAIYAYPLLSPGYTNIEQYFIKQK"
     gene            894..1613
                     /locus_tag="P5310_03700"
     CDS             894..1613
                     /locus_tag="P5310_03700"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HRU32498.1"
                     /translation="MKNNQFLLALVIGLSIIISASIVSYTFYRIKGMDNTLSVTGSTR
                     IRVNSDIVKWVGEFSRTVPVDSLKAGYQEMGKDLKVVLEFYKKNGIDPEDLIISPVFL
                     EQLGLYGDRQTPREYLLRQTVEIQSSDLEKIGELSKNLQGLIDSGVAFRTQSLEYYYS
                     KLPELRVNLLTEAMNDAKARAEKIAQSSGQGVGSLRSASMGVVQVLEPNSTEVSDYGS
                     YDTSTIEKEVMVTVRATFLLK"
     gene            complement(1654..2718)
                     /locus_tag="P5310_03705"
     CDS             complement(1654..2718)
                     /locus_tag="P5310_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRU32499.1"
                     /translation="MPEGSMTSRERWEAVLRGQKPDRLPMDYWATTEATERLMRYLNC
                     SNPRELYKKLHIDRPVDVGPVYTGPKLEPQTDMYGCIYRDVDYGTGVYSECIYHPLAK
                     YDTVEEIEKNYVWPTVDWFDYSVIPEQIKGMEEYPIRGGGSEPFLTYKNLRGQEQAFI
                     DLVLNPEIVDYCLDKLFGFCYENTLRIYEQIPGKITFSYVAEDMGGETDLMFSPEHIR
                     RFLLPRMKRMVELAHRSGVYAFHHNDGAIRRIIPDLIGIGIDILNPIQWRCKGMDREG
                     LKRDFGDKLVFHGGVDNQYTLAFGSVDEVKKEVIENIKILGYDGKYIIAPCHNIQSIS
                     PPENIVTMYETGYEYGWMRE"
     gene            2837..3271
                     /locus_tag="P5310_03710"
     CDS             2837..3271
                     /locus_tag="P5310_03710"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRU32500.1"
                     /translation="MSYIMMNEEIDILLNNVMALIKSRNRRLMESYGITPLQYRALSL
                     IKDKNPTMGDLCNLLYLSSSSVTDLVDRIESNRLARRIRSSEDRRVITLEITEEGISL
                     LEEIREKESEVLSKSLNLLDEETKIRIKYALEELYNVLKKED"
     gene            3273..>3852
                     /locus_tag="P5310_03715"
     CDS             3273..>3852
                     /locus_tag="P5310_03715"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRU32501.1"
                     /translation="MEEKGTLETEESILQEEAGTTILPEYLDRKHIRSRVISLSLPAL
                     LNMFLVTFVGMADIIMVGRLGASAIAAVGIVNQPIFLVVSVFIALTVGTTALVARFIG
                     AKDINSAKDVSKQSLVMSIFFGIAILGFLYSFATQITRAMGAEPDVVILGATYLKIVA
                     LGMPFNIVSMIIAAILRGSGDMKSPLISDIVAN"
BASE COUNT         1203 a          749 c          786 g         1114 t
ORIGIN      
        1 atctggggat gggtgatcca gcttataaac tgttacctca gtctatgtgg gaggacaaaa
       61 acttaagaag cctctatgag tataatccag caatggcaaa tcaacttctg gatgaacttg
      121 gattaaagaa agataagagc ggctatagag taagaccaga cggtaaacct cttactctaa
      181 ctatactggt aaatctggca tacccaatac atccagatgt agcagaaata gtacaaaaac
      241 aatgggaagc agttggaata aaggttgcca tagattccat aagtggagct ttatggtggc
      301 ccaggataaa taccagtgaa taccagatag tagcatatga gctggactat acaacggaca
      361 gatatttctt aacctactct cgtagtttta taccaaatgc taattctacc tactgggctc
      421 ctctatgggg gctatggtat gcttcagatg gtaaagcaga agggtcagaa aaaccgacag
      481 gcgatgccct taaactgata gctctctttg ataagataaa ggttaccact gaagaaaagc
      541 agaaggcgaa actaacagct gatgcagtta gcctgtgcgc aaaggatcta tttgctattc
      601 ctaccatagc tggataccct caaccatgca tagtgaaaaa taactttaaa aatgttccta
      661 agaatgccat atatgcatat ccattattat ctccaggata tacaaatatt gaacagtatt
      721 ttataaaaca gaaatagtat gggataagtt aggattgcgg gggcaattta taaattgccc
      781 ccgcaggata caaaaacgat caatctcttc tatgagaaga agatagtctc ctatctttta
      841 ttcttcaccg gaaagtgata agattgagag aaagatgaag agggagggga tatgtgaaga
      901 acaaccaatt tctattagca ttggtgatag gtttaagtat tatcatcagt gcatctattg
      961 tttcctatac attttacaga ataaagggta tggataatac cctcagtgta acaggttcaa
     1021 ccaggataag ggtgaattct gatatcgtaa aatgggtggg ggaattcagc aggactgttc
     1081 ctgtagactc tctcaaggcg gggtatcagg agatgggtaa agaccttaaa gtggtcctgg
     1141 agttttacaa aaagaatggc attgaccctg aagacctgat aatttcacct gtctttttag
     1201 aacagctggg gctttacggt gaccgtcaga ctcccagaga gtaccttctc aggcagacag
     1261 tggaaatcca gtccagtgat ctggaaaaga tcggtgagtt atcaaaaaat cttcagggat
     1321 tgattgatag cggagtagca ttccgcactc agtctcttga gtactattac tccaaacttc
     1381 cagaattaag ggtgaacctg ctcacagagg ctatgaatga tgccaaggca agggcagaaa
     1441 agattgctca gagcagtggg caaggggttg gcagtctgag atctgccagt atgggagtag
     1501 ttcaggtatt ggaaccaaac tctacagaag taagtgacta tggatcttat gatacatcaa
     1561 caatagaaaa agaagtgatg gttacagtaa gggcgacgtt tttactgaag taataagtaa
     1621 agggggtggg atattccacc ccctttttgt tttttattcc cgcatccatc catattcata
     1681 accggtctca tacatagtaa caatattctc cggtgggctt atggattgaa tattatgaca
     1741 gggagcaatt atatacttcc catcatatcc aagtatcttt atattctcta taacctcttt
     1801 cttcacctcg tctaccgaac cgaaggctaa agtatactga ttgtcaacac ctccatgaaa
     1861 caccagttta tctccaaagt ctctctttaa tccttctcta tccataccct tacatctcca
     1921 ctgtatcgga ttcagaatat ctatgcctat tcctatcagg tccggtatta ttctccttat
     1981 tgcaccatca ttatgatgaa aggcgtatac acctgaccga tgcgccagct caaccatccg
     2041 tttcatacgg ggaagaagaa accgtcttat atgttcagga gaaaacataa ggtcagtctc
     2101 tcctcccata tcctcagcca cataggaaaa agtaatctta ccaggtatct gctcatatat
     2161 tctcaatgtg ttttcataac agaatccaaa gagtttatcc agacaataat ccacaatctc
     2221 agggtttaat accaggtcta taaatgcctg ttcttgacct ctcaaattct tatatgtcag
     2281 gaatggttcc gacccaccac ctctgattgg atattcctcc atacctttta tctgttcagg
     2341 gattacgctg taatcaaacc agtcaacagt tggccagaca taatttttct ctatctcttc
     2401 cactgtatca tacttagcta aaggatggta tatacactct gagtataccc ctgtaccata
     2461 gtcaacatct ctgtagatac aaccgtacat atcagtttga ggctccagct tcggacctgt
     2521 atatacaggt cctacatcca caggtctatc tatgtgtaat tttttgtaaa gctctctcgg
     2581 gttgctacag tttagatatc tcatcaatct ttcagtagct tcagtggtag cccaataatc
     2641 cataggaagt ctgtcaggct tttgtcccct taataccgcc tcccacctct ctcttgacgt
     2701 catagatcct tctggcataa ttttctcctc cgttaatctc taatctatat cacccatttt
     2761 agcataaaaa taccccagaa ccactttaca tctatttcgt tttagggtaa aatacttcaa
     2821 gatacgaaat atctgtatgt cgtatattat gatgaacgaa gagatagata tattactgaa
     2881 taatgtaatg gctttaataa agagtaggaa cagaagattg atggagagtt atggcattac
     2941 tcccctacag tacagggctt taagtcttat aaaagataaa aaccctacta tgggagacct
     3001 ctgtaatcta ctttatctct cctcaagttc agttaccgat cttgtagata ggattgaaag
     3061 taatagactg gctaggagga tacggagtag tgaagatcga agagttataa cccttgagat
     3121 aactgaagaa ggcatatctc tgcttgaaga gattagagag aaagaaagcg aggtattatc
     3181 caagagtctg aatcttctag atgaagagac aaagatcagg ataaagtatg ccctggaaga
     3241 actctacaat gtattaaaga aggaggatta aattggaaga aaaaggaacg ctggaaactg
     3301 aagaatccat attgcaagaa gaagctggta ctaccatatt accagaatat ctggacagaa
     3361 agcatatccg ctccagagta atttccctct ctttaccagc tcttttgaat atgttccttg
     3421 taacttttgt aggtatggcg gatataatta tggttggaag gttaggtgct tcggctatag
     3481 ccgccgtagg gatagtgaat caaccaattt ttcttgttgt ctcggtattt atagctctta
     3541 ctgttggtac cactgcactg gtagccagat ttataggtgc aaaagatata aatagtgcga
     3601 aagatgtctc aaaacagagt cttgtaatga gtatcttttt tggtatagcg atacttggct
     3661 tcctatattc atttgctact cagataacga gggctatggg tgcagaaccg gatgtagtaa
     3721 ttcttggagc aacttatctg aagatagtag ctttagggat gccatttaat atagtaagta
     3781 tgattatagc tgcgatactg agaggttcgg gggatatgaa gtctccactt atatccgata
     3841 tagtagctaa tc
//
LOCUS       DAOTKU010000114         3827 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_20103_length_3827_cov_4.215801, whole genome shotgun sequence.
ACCESSION   DAOTKU010000114 DAOTKU010000000
VERSION     DAOTKU010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3827
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20103_length_3827_cov_4.215801"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(228..1370)
                     /locus_tag="P5310_03720"
     CDS             complement(228..1370)
                     /locus_tag="P5310_03720"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRU32502.1"
                     /translation="MKTKHTYFFISTFLFWVSLYLYVPILSPYAENLGSSLQMIGFIV
                     GSYGFTQFILRIPLGIWSDKIQKRKPFIILGFIFSILSGLGLAISKSPTGLLIFRGLS
                     GVSATTWVAFTTLFSSYFDPSRVIASLSLLNFFTQAGQLVATLLGGFIAQNMGWTFPF
                     YFAALTALLGLIPALFIKEDSYKPASISIAELLSVGKNTLLLVASLLAILVQYFSFVT
                     TYGFLPVYATSIGLSKSALGILTFLSSVPAAIASLLNSGPLFSKINKKYLLTSGFILA
                     MVSSLFTPFTKSFLILGILQCLGGIARGINYPVLMGLSIIEIPVEKRATAMGFFQALY
                     GIGMFGGPFIGGLIGSIWGINSIFISTAALMLFSAILTLLLVKIDS"
     gene            complement(1390..1476)
                     /locus_tag="P5310_03725"
     tRNA            complement(1390..1476)
                     /locus_tag="P5310_03725"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1440..1442),aa:Ser,seq:tga)
     gene            complement(1478..1819)
                     /locus_tag="P5310_03730"
     CDS             complement(1478..1819)
                     /locus_tag="P5310_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH938/NDUFAF3 family protein"
                     /protein_id="HRU32503.1"
                     /translation="MNIEQYEFGKITIDGISYFSDIIIYPDHIEDEWWREEGHELHTV
                     DIMEIVETKPKVLIVGTGAYGMLRVLPEAEKYLKDNNIQLIAELTGRAVEIYNQTGDK
                     SKVVCALHLTC"
     gene            complement(1816..2826)
                     /locus_tag="P5310_03735"
     CDS             complement(1816..2826)
                     /locus_tag="P5310_03735"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HRU32504.1"
                     /translation="MGYNYLFSPATIRGLHFKNRIVMPPMATNFGIVTEESIAYYTKR
                     ALGGVGSIIVEGIPTHLFHNKKFQERLKLLSNGIHRGGANAFVQLTIKSNPQPRRDVT
                     DIPLDEIKSIIDDFIFAGKVILDAGFDGVEPHGAHGFIMNRFFSPIFNRRNDEYGGDL
                     ERRMKFGFDVVSGLKSIAGDKFLVLYRHTPAEFRAGGYTIEDTILFGKRLKEAGLDIF
                     DISPSTAPGSYDYGALARQVRAGVDIPVIMVGKMYVPELADSRIRDGYADFVAIGRSI
                     LSDPFLPKKIQDGRFDIINSCKMCNIKCHGYLRRGVPISCTMNPSVGREYLEKGLWRA
                     LR"
     gene            complement(2836..3792)
                     /locus_tag="P5310_03740"
     CDS             complement(2836..3792)
                     /locus_tag="P5310_03740"
                     /inference="COORDINATES: protein motif:HMM:NF014610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HRU32505.1"
                     /translation="MRALGLYSGGLDSVLAIKIMMSHGIDVIPVAFFTPFFSPDTGYG
                     EKVLGRAIEQIDITEEFIDILLSPRYGYGKNMNPCIDCHSLMIKKAKELLPLYGASFI
                     ITGEVLGERPMSQNKQALEKVVRRASAEGLVVRPLSGALLPGTIPEEKGWIKREWLYA
                     ISGRKRDRQMELAATFGIEEYKTPAGGCLLTDMNFSKRLRFLIGIHPRPAREELELLK
                     VGRHFYLDGCWIIIGRNERENKILRESNAWEYVFFTIDYPGPTGIIVEGSYIDQAVRI
                     LIRYSDAPKDRSTRVRVIENSGKSYILESLALEDEEIERIRL"
BASE COUNT         1088 a          909 c          699 g         1131 t
ORIGIN      
        1 ctcagttgca tgacctatgt atcttgagaa ctcatactca aaggaaatcc cccaggttct
       61 cagcctttcc aggatcttct ctgtaagact cctccctctt ttccctcctg ctaccgggtt
      121 tattattaat ttaaaacgca tagacctcct ctacatggat atatatactt atttaaaaat
      181 attttcaggc atctattata tcacagagat aggaataaga gtcaatgtta agaatctatt
      241 tttactagaa gtagtgtcaa tatggcagag aaaagcatca gagctgctgt agagatgaat
      301 atactattta taccccagat ggaccctata agtcctccta taaaaggacc accaaacatc
      361 cctatcccgt acagagcctg gaagaatccc atagcggtag ctctcttttc taccggaatt
      421 tctattatgc ttaaacccat aagtacagga tagtttatcc ctcttgctat tccgcccaga
      481 cactgaagaa taccaagaat taagaaactc tttgtaaagg gggtaaatag agaagagacc
      541 atcgcaagga taaatcctga agttaaaaga tacttcttat tgattttgga gaagagcggt
      601 ccactgttga gtaaagatgc gatagctgca gggacagatg agaggaatgt caatataccc
      661 agagcacttt tagataaccc tatactggtg gcatatacag gcaagaatcc atatgttgtg
      721 acaaaactga aatactggac aagtatagct agaagtgaag ccaccaggag aagagtgttt
      781 ttccccacgc tgaggagttc tgctatagaa atactggcag gtttatagga gtcctctttt
      841 ataaaaagag cagggattaa tcccaaaaga gcagtgagag cggcgaagta gaaggggaat
      901 gtccaaccca tattctgggc tataaatccg ccaaggagtg tggctacaag ttgtccagcc
      961 tgtgtaaaga aatttaaaag gcttaaagat gcgatcaccc tggaggggtc gaagtagctt
     1021 gagaacaggg tagtaaaagc tacccaggtg gtagcggata caccgcttaa tcctctgaat
     1081 atcaatagcc cagttggaga ttttgatatg gctaatccaa gaccactgag gatagagaat
     1141 ataaaaccca gtataatgaa tggttttctc ttttgaatct tatctgacca tatccccagg
     1201 ggtatcctca ggatgaactg cgtaaaaccg taagaaccaa caataaaccc tatcatctga
     1261 agagaactgc caagattttc tgcatacgga gaaagtattg gcacatagag atagagagag
     1321 acccagaaga gaaaggtaga gataaagaag tatgtgtgtt ttgtcttcac ctttttatct
     1381 tttcttttat ggcggagggg gagggattcg aacccccggg cctcatcagc caacggtttt
     1441 caagaccgcc gcaataaacc actctgccac ccctccacta acaggtaagg tgcagggcac
     1501 acaccacttt actcttatct ccagtttgat tatatatctc aaccgccctg ccagtcaact
     1561 ccgctataag ctggatgtta ttatccttca gatacttctc cgcctctggc agtactctca
     1621 gcatgccata agcccctgtt cctactatga gcaccttggg ttttgtctct actatctcca
     1681 taatatcaac tgtgtgtagc tcatgtcctt cttctctcca ccactcatcc tctatgtggt
     1741 caggatatat aattatatca gaaaaatatg atattccatc tattgtaatc ttaccaaact
     1801 catattgttc tatattcatc ttaatgctct ccataaacct ttctcaaggt attctctacc
     1861 cacagaagga ttcattgtgc atgaaattgg tacccctctt ctcaggtatc cgtgacactt
     1921 tatattacac atcttacaac tgttgataat gtcaaatcta ccatcctgaa tctttttggg
     1981 aaggaatggg tcagatagaa tgctccttcc tatggcaaca aagtctgcgt aaccatctct
     2041 tatcctgcta tctgcaagtt ccgggacata catcttgcca accataataa caggtatatc
     2101 cactccagct ctaacctgcc tggcaagtgc tccatagtca tacgagccgg gcgctgtgct
     2161 tggagatata tcaaatatat ccagtcccgc ctctttcagc cttttcccaa ataatatggt
     2221 atcttctatt gtatatccac cggctctgaa ctctgccgga gtatgcctgt agagtaccag
     2281 aaacttatct cctgctatgg attttaatcc acttaccaca tcaaatccaa acttcattct
     2341 tctttccagg tctccgccat attcatcatt acgtctattg aatatcggtg agaagaaccg
     2401 attcattata aaaccgtgag ccccatgagg ctcaactcca tcgaatcctg catccagaat
     2461 aaccttcccg gcgaatataa aatcatctat tatggacttt atctcatcca gaggtatatc
     2521 ggttacatcc cttctcggtt gcggattact ctttatagtt aactggacga aggcgtttgc
     2581 tccgcccctg tgtatgccat tggataagag tttcagtctc tcctggaatt tcttattgtg
     2641 aaaaagatgg gttgggatac cctccactat aatgctccca actccaccca gggctctctt
     2701 tgtataatat gctatggact cctctgtcac tataccaaaa ttggtagcca taggaggcat
     2761 aactatcctg ttcttaaagt gtaaacccct gattgtagca ggagaaaaca gataattgta
     2821 acccataaat tcacctcaca gtcttattct ttcaatctcc tcatcttcta gggcaagaga
     2881 ttctaagata taacttttgc ctgaattttc tataactcta actctggtcg atctatcctt
     2941 tggagcatca ctgtatctta ttaaaattct aactgcctgg tctatataac taccctctac
     3001 tattatacca gtaggtcctg ggtaatcaat agtaaagaag acatattccc atgcatttga
     3061 ctctcttaat atcttattct ccctctcatt gcgtccgatt ataatccagc atccatccaa
     3121 gtagaagtgt cttccaactt taagaagttc aagctcttct cttgcgggtc ttggatggat
     3181 accgatgaga aatctcagcc ttttagagaa gttcatatct gtgaggagac atcccccggc
     3241 tggtgtctta tattcctcta ttccaaatgt tgccgccagc tccatctgtc tatccctctt
     3301 tctccctgag attgcatata gccattctct ctttatccac cccttttctt ccggtatggt
     3361 acccggaagt agtgcccctg atagaggtct aaccaccagt ccttctgctg atgcacgtct
     3421 gacgaccttt tctaaagcct gtttattttg ggacataggt ctttcgccaa ggacctctcc
     3481 ggttattata aagcttgccc catagagagg tagaagttcc ttcgcctttt taatcataag
     3541 actatgacaa tctatacagg gattcatatt ctttccatac ccatacctgg gggataagag
     3601 tatatctata aattcttcag tgatatctat ttgttcaatg gctctgccga ggactttttc
     3661 cccataccct gtatccggtg aaaaaaatgg tgtaaaaaat gcgacaggga ttacatcaat
     3721 cccgtgactc atcattatct ttatcgctag gacgctatca agcccaccag agtacagccc
     3781 taatgccctc attcttcaag cattctcctg taagactcca gaagacc
//
LOCUS       DAOTKU010000115         3784 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_20376_length_3784_cov_2.903459, whole genome shotgun sequence.
ACCESSION   DAOTKU010000115 DAOTKU010000000
VERSION     DAOTKU010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3784
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20376_length_3784_cov_2.903459"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="P5310_03745"
     CDS             complement(<1..524)
                     /locus_tag="P5310_03745"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HRU32506.1"
                     /translation="MGEEVFLIDWILLNLTGRYRLIRELFSRGISTEDIISSLELGQE
                     KLSLLRKKAGEEYKKAVDSGIELIPLSSDDYPYLLREISDPPALLYLLGERVWLNGSF
                     VSVVGTRKPSRYGINITKKIVSKLVENGIGIVSGFAYGIDTVAHMEALNGGGYTIGVL
                     GTGVDIVYPGVNKG"
     gene            complement(503..2035)
                     /locus_tag="P5310_03750"
     CDS             complement(503..2035)
                     /locus_tag="P5310_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HRU32507.1"
                     /translation="MLAKVISATLSGIEGVPVDVEVDISNGLPSFTIVGLPDQSVQES
                     RERIRSAIKNSDLNFPVKRITVNLAPADIKKEGPAFDLPIAIGILIASEALAPKMEEP
                     FLIVGELSLDGTIRPVNGIISIAIMARDLKIPVMFVPYENSSEAALIPGIKVYGIKNL
                     TDLVQFLNGAISLSPSEPARSIKINEFSGDFNEVKGQESAKRALEIAAAGFHNVIMVG
                     PPGSGKTMLAQRLPTILPPLTEEEALEVTRIYSSAGLLPKGGGIIEERPFRSPHHSIS
                     LAGMVGGTSNPKPGEISLAHNGVLFLDEFPEFRRDVLEALRQPMEDGSLVISRAHGSI
                     RFPARFMLVASMNPCPCGYLGDTKKNCTCTQYQIEKYFSKISGPILDRIDIQIEVPRL
                     ETDTLIRNTPGEGSNFIRDRINRVREFQIDRFKDGFRFNGLMTPREIKKYCRLNPPVQ
                     SLLREIIDKFHLSARSYDKLLKVARTIADLNESEFIREEDILEAIQYRILDRKYWEKK
                     FS"
     gene            complement(2025..2417)
                     /locus_tag="P5310_03755"
     CDS             complement(2025..2417)
                     /locus_tag="P5310_03755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HRU32508.1"
                     /translation="MKNKRALGNLGEKKALEYLRNLGYDILAVNFYSQRGEIDIIAED
                     KGTLSFVEVKTRTGKKFGDPEESITLKKRERIVRTALYYLHSRGWPRLSCRFDVLTVK
                     IDPKTGDTEFNLTRDAFQLEGDTYRYVG"
     gene            complement(2407..3048)
                     /locus_tag="P5310_03760"
     CDS             complement(2407..3048)
                     /locus_tag="P5310_03760"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HRU32509.1"
                     /translation="MASRKNKLNKVRPNQGIELSLYSNGIYPVCGIDEAGRGALAGPL
                     VAAAVILPRRCRIPLNDSKKLSPEEREVIALRLKEEAVDIGLGIVESEEIDRYGLSWA
                     NKEAFKRAVRDLTVEIKGMVTDALHIDMDIFQINLINGDEICSSVSAASIIAKVTRDH
                     IMEYYDKIFPQYNFSRNKGYPTPDHKERIRIFKPSVIHRVSFSPVREVMNYEE"
     gene            complement(3015..3536)
                     /gene="lepB"
                     /locus_tag="P5310_03765"
     CDS             complement(3015..3536)
                     /gene="lepB"
                     /locus_tag="P5310_03765"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HRU32510.1"
                     /translation="MNEKEKALVRDLIESVIIAVVLAFGIIRPFIIQPYAIPSGSMEP
                     TLLVGDKLFVNKFIYRFKPIQRFDVIVFKYPHNPKVDYIKRVIALEGETVQLIDGKVY
                     INGQPVEENHKMYSSYDNFGPITVPKGNLFVLGDNRGNSEDSRYFGFVPVENVIGKAF
                     LIYWPPGRISLIK"
     gene            complement(3529..>3784)
                     /gene="rplS"
                     /locus_tag="P5310_03770"
     CDS             complement(3529..>3784)
                     /gene="rplS"
                     /locus_tag="P5310_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HRU32511.1"
                     /translation="EGGRERLQFFEGTVISIKGSGVNRAFTLRKISYGVGVEKVFPLY
                     SPIIDRVEVIRKGKVRRAKLYYLRSKIGREARIKEANINE"
BASE COUNT         1052 a          939 c          622 g         1171 t
ORIGIN      
        1 aaccccttat ttacccctgg atacactata tctacccctg ttcccagaac acctatcgta
       61 taaccccctc cgtttagcgc ttccatatgg gcaacggtat ctatcccata ggcaaaacca
      121 ctgacgatac ctatcccatt ttcaaccagc ttagaaacta tctttttggt aatatttata
      181 ccatacctgc ttggcttacg agtccccacc acacttacaa agcttccatt tagccagacc
      241 ctttcgccca gaagatatag aagagctgga ggatcagaga tttctctcaa aaggtatgga
      301 taatcatcag atgataatgg tatcaattca atccctgaat ctacagcctt tttatactcc
      361 tctcctgcct tttttctgag aagagagagt ttctcctgtc caagctccag agagctaata
      421 atatcttcag tagaaatacc tctggagaat aattctcgta ttaatctgta ccttccggtc
      481 agatttagaa ggatccagtc tattaagaaa acttcttctc ccaatacttc ctgtccaaaa
      541 tcctgtattg aatagcttcc aggatatcct cttcccttat gaactcactt tcgttcaggt
      601 cagcaatagt ccttgccact tttaaaagct tatcataaga acgagcactg agatgaaact
      661 tatctattat ctccctcagg agagactgaa cagggggatt aagcctgcag tacttcttta
      721 tctctctggg ggtcataagc ccattaaacc tgaaaccatc cttaaatcta tctatctgga
      781 actctctcac cctgtttatt ctatctctta taaaattaga accttcccca ggtgtgtttc
      841 ttataagtgt atccgtctcc aacctcggaa cttctatctg tatatctatt ctatcaagga
      901 taggaccaga aatcttactg aaatacttct ctatttggta ctgggtacag gtacagttct
      961 ttttggtatc tccaagatat ccacagggac agggattcat actcgcaaca agcataaacc
     1021 tggcagggaa tcttatactt ccatgcgctc tggatataac cagactccca tcctccatag
     1081 gctgtctcaa tgcttctagg acatctcttc tgaactcagg gaattcatcc agaaagagaa
     1141 caccattatg agccaaactt atctcccctg gcttcgggtt gctcgttcca ccaaccatac
     1201 cggcaagtga aatggaatgg tgaggagacc tgaatggtct ctcctcaatt attcctcctc
     1261 ccttggggag taatcctgca ctgctataga tcctggtaac ttccaatgct tcttcctctg
     1321 tgagaggagg aagtatggtt ggtaatctct gagccagcat ggtcttcccc gacccgggag
     1381 gaccaaccat tattacatta tgaaaaccag cagcggcaat ctccaacgcc ctcttggcag
     1441 attcctgtcc ttttacctca ttgaaatctc ctgaaaattc atttatcttt atagacctgg
     1501 caggttcaga tggagaaagg gatattgctc cattcagaaa ctggacaagg tcggtaagat
     1561 ttttgatacc atacaccttt ataccaggta taagagcagc ttccgagctg ttttcatacg
     1621 ggacgaacat aacaggaatc tttaaatccc ttgccattat agctatagat ataataccgt
     1681 taacaggtct tatagtacca tctaaggaaa gttcgccaac tataagaaat ggttcttcca
     1741 tctttggagc taaagcctca gatgcgataa gaatccctat agctattggc aggtcaaacg
     1801 ctggaccttc tttttttata tcagctggag ccagatttac agtaattctc ttaacaggaa
     1861 agtttaaatc agaattcttt atcgcagagc gtatcctctc tcttgactcc tgaacagact
     1921 gatcaggaag ccccactata gtaaagctag gaagtccatt acttatatcc acttctacat
     1981 ctaccggtac accctctata cctgagaggg tagcgcttat taccttagcc aacatacctg
     2041 taagtatccc cttcaagttg aaaggcatcc ctggtaagat tgaattcagt atctccggtc
     2101 tttggatcta tcttcactgt aaggacgtca aatctgcagc ttaatctggg ccatcccctg
     2161 gaatggagat agtaaagagc agtcctcact atcctctccc tttttttcaa tgttatggac
     2221 tcctctggat ctccaaactt cttaccggtt ctggtcttta cctctacaaa gctcagggtt
     2281 cctttatcct ctgctataat atctatctct ccacgctgag agtagaaatt tacagcaagg
     2341 atatcgtacc ccaaattcct taaatactcc agagcctttt tctcccccaa atttcccaga
     2401 gctcttttat tcttcatagt tcatcacctc tcttacaggt gaaaaagaaa ccctgtgaat
     2461 tacagatggc ttaaatatcc ttatacgctc tttgtggtca ggggtaggat accctttatt
     2521 acgagaaaaa ttatactggg gaaaaatctt atcgtagtat tccattatat gatcccttgt
     2581 tactttggct ataatagatg ctgcagaaac agaagagcat atctcgtctc catttatgag
     2641 atttatctgg aatatatcca tatctatgtg taaggcgtcg gtcaccatcc cttttatctc
     2701 tacagttaaa tctcttacag cccttttgaa agcctcttta ttagcccagg agagaccgta
     2761 acgatcaatt tcttcagatt caacgatacc taaacctata tctacagctt cttcttttaa
     2821 tctgagagct ataacctctc tctcttctgg agatagcttt ttagaatcat ttaggggtat
     2881 tctacatctt ctgggcagaa taacagccgc cgccactaaa ggtccagcaa gagcccctct
     2941 tcccgcctca tctattccgc atacaggata gattccgttt gagtacaggc taagctctat
     3001 cccttgattt ggtcttactt tattaagctt attcttcctg gaggccaata tatcaaaaat
     3061 gccttaccta taacattctc aaccgggaca aaaccaaaat atctgctatc ctcgctgtta
     3121 cccctgttat cacccaagac gaagaggttc cccttcggaa ctgtaatagg tccaaaatta
     3181 tcataagaag aatacatctt gtggttctcc tctacaggtt gcccatttat ataaacctta
     3241 ccatctataa gctgaacggt ctctccttca agagctataa ccctttttat atagtctacc
     3301 ttgggattat gtggatactt aaatactatt acatcgaagc gttggatggg tttaaatctg
     3361 tagataaact tatttacaaa aagcttatcc cctacgagaa gagtaggctc catagatcca
     3421 cttggaatag catagggctg gataataaag ggtcttatta taccaaaagc cagaactaca
     3481 gctattatta cggattctat caaatctctt accagagcct tctccttctc attcatttat
     3541 atttgcttcc tttattctcg cctctctacc tatcttactt ctcagatagt aaagcttagc
     3601 cctccttacc ttaccttttc tgataacctc taccctatca attattggag aatagagagg
     3661 gaataccttc tctaccccaa caccataaga tatctttctc agggtaaaag ccctgttaac
     3721 tccactgccc tttattgaga ttacggttcc ttcaaaaaac tgtaatctct ctcttccacc
     3781 ttct
//
LOCUS       DAOTKU010000116         3777 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_20423_length_3777_cov_2.905427, whole genome shotgun sequence.
ACCESSION   DAOTKU010000116 DAOTKU010000000
VERSION     DAOTKU010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3777
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20423_length_3777_cov_2.905427"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..346)
                     /locus_tag="P5310_03775"
     CDS             complement(50..346)
                     /locus_tag="P5310_03775"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32512.1"
                     /translation="MKDRDIEKIIEILRESVKEWKQPVVTQIAERDRDPFRILISTIL
                     SLRTKDEVTKIASERLFSLARTPEEMLRLEEEEIERAIYPVGFYRNKAKTIKEI"
     gene            complement(353..1684)
                     /locus_tag="P5310_03780"
     CDS             complement(353..1684)
                     /locus_tag="P5310_03780"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HRU32513.1"
                     /translation="MRRFLVITLCILFSLSLLSDYSIANTLIYADISPPNEYINETLI
                     ALVNMGSLYVDSTPRGASIYLDNIYEGETPRRIDSITTGIHTLRLSKDGYRDYVQQVR
                     IDAQQTANIQISLTPGYSLTVNSTPRGAMVYIDGIFKGITPVSTPITPGYHSVRLVLE
                     GYLDYQDSIRVTGDTNLNISLQIGEGSLVVFSEPSSADVYVDDKYYGRTPITIQKIPA
                     GKRMVTVRLSGYADWSDEVVIEPGKIGQVNVKLKLAGTLQVSSSPSGAKVYLNGKYIG
                     ETPLSNSNIPEGTHNLSVELFGYKPWQGIIEVSPGQITNISARLESASIGISDIDISP
                     QPYKVKTLFKRDLSIKFNITRPAMVTVEIRDVNDKLIATPMAGFKASTPKLDVKWRGD
                     LSPGNYKVVVIAIDENGNQVGREAIFRAEEESFLIWILVGLLGILVLWLLF"
     gene            complement(1701..2213)
                     /locus_tag="P5310_03785"
     CDS             complement(1701..2213)
                     /locus_tag="P5310_03785"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HRU32514.1"
                     /translation="MDEKLKRLIEQAIQMEKDGITFYEDLSAKVTYESAEKMVLSFAD
                     DERRHLRILNDIFSNILSMDIEKYIAENLPGEKVKTIFDDVKGKVDVKDVSDELSALK
                     LAMDMEERSYKLYDEALSLETDESLKKLWKRLADEERRHYDIFWNTYDFIANPEEWVL
                     REERGLLDGG"
     gene            complement(2271..2828)
                     /locus_tag="P5310_03790"
     CDS             complement(2271..2828)
                     /locus_tag="P5310_03790"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010867811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HRU32515.1"
                     /translation="MNELELLRQEVEACTKCDLYKTRTRVVFGDGNPNAKLLIVGEAP
                     GYYEDKRGLPFVGSAGKLLDKLLEEIGLNRDKVYIANVIKCRPPNNRDPNIDEVSACS
                     PYLFAQISYISPRVIITMGNHSTRLLAETDLGMTKVHGRVFKKGDKVVLPTFHPAACL
                     YHPEWLKLSREDLAKIPDLLSKKDL"
     gene            complement(2794..>3777)
                     /locus_tag="P5310_03795"
     CDS             complement(2794..>3777)
                     /locus_tag="P5310_03795"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRU32516.1"
                     /translation="MMGRNPYVSRFRGETGSDEDIVREAMRLTNTIDFADRPITELSG
                     GEKQRVILARALAQQPKILLLDEPTSHLDISYQIEMLDLIKRLVKEKGISVLSALHDP
                     NLASQFCDKIILMKDGTIYDFGPPRDVLTSANLKEVFNIDVIVRQHPVYDGIYILPLS
                     RSSGGYRDLDIKVHIICGGGTGAKILYLLHRYFDLSCGVVNLLDSDADVCNELGIPAV
                     FEAPFSPISDENYQKNLNMIDRSDVVVISSLPFGKGNLANLKSFGYAIEKGKLTIMID
                     DPPIEDRDFTDKEATCLWNSSREKSIVLRSNEIEKIPEIIYERAGVIKTRG"
BASE COUNT         1016 a          903 c          613 g         1245 t
ORIGIN      
        1 catctatatc atccggaact ctaccattat atctctctat caaaaccttt catatttctt
       61 ttatagtttt agccttattt ctatagaacc ctacaggata tatagccctt tctatctcct
      121 cttcctctaa tctgagcatc tcttcaggtg tcctggctaa tgagaagagt ctctcactcg
      181 ctatttttgt tacctcatcc tttgtcctaa ggctcagtat ggtagatata agtatgcgga
      241 aagggtctct atccctttcc gctatttggg tcacaactgg ttgcttccac tcttttacag
      301 attctctcag tatctctatt atcttctcta tatctctatc tttcacagta aactaaaaga
      361 gtaaccagag gacgagaatt cccagtagac caacaagtat ccagataaga aagctctcct
      421 cttctgctct aaatatagcc tctctaccca cctgattccc attttcatcg atagctatta
      481 ccacaacctt atagttacca ggtgatagat cccccctcca cttgacatct aatttcggag
      541 tagaagcttt gaatccagcc ataggggtag ctataagttt atcattaaca tctcttatct
      601 ctaccgtaac catagctggt ctggttatat taaattttat agaaaggtct ctcttaaata
      661 gagtctttac cttgtatggc tgaggagata tatctatatc cgagatacct atagaagctg
      721 actcaagcct tgcggatata ttagttattt gtcctggtga aacttctatg ataccctgcc
      781 agggtttgta accaaagagt tccacactga gattatgggt tccttcaggg atattagagt
      841 tagataaagg ggtttctcct atatattttc cattgagata aacttttgcc cctgacggac
      901 tactggatac ctgcagggtg ccagccagtt tcaatttaac gtttacctga cctatctttc
      961 caggctctat tacaacttca tcagaccaat ccgcataacc tgataatctt acagttacca
     1021 tcctcttgcc tgcaggtatc ttctgtatgg ttataggagt tctcccgtaa tatttgtcat
     1081 ccacatatac atctgcactt gaaggttctg agaataccac cagggaacct tcccctatct
     1141 ggagacttat attcaggttt gtatctccgg ttacccttat cgaatcctga taatcgagat
     1201 atccttcaag caccaatctt acactgtgat atccaggagt gataggggta gaaacagggg
     1261 ttatcccttt aaatattcca tcgatataga ccattgcacc tctcggggta gaatttaccg
     1321 tcaagctata tcctggggta agacttatct gtatatttgc tgtctgctga gcatctatac
     1381 gtacctgctg gacataatcc ctgtatccat ctttggagag tcttaaggta tggattcctg
     1441 tggtgataga atcaatcctt cttggagttt ctccctcata gatgttatcc aggtatatac
     1501 ttgcccctct cggagtagaa tctacgtata gagagcccat attcacaaga gctattagag
     1561 tctcatttat atactcattg ggaggactta tgtccgcata tatcagtgta ttcgcaattg
     1621 aataatcaga taataaactt agggaaaata atatgcacag cgttataacc agaaaccttc
     1681 tcaaaaaatc acctccttat ttagcctcca tcaaggagac ctctctcctc tctcaacacc
     1741 cactcttctg gattggctat aaaatcatag gtattccaga atatatcata gtgtcttctc
     1801 tcctcatcgg cgagtctttt ccatagcttc tttaaactct catccgtctc taaacttaaa
     1861 gcctcgtcat agagtttata acttctctcc tccatatcca tggcaagctt aagagcagag
     1921 agttcatctg agacatcttt tacatccacc ttccccttga catcatcaaa gattgtcttt
     1981 accttttcac ctggcagatt ctcagcaata tatttttcta tatccataga taggatatta
     2041 ctgaagatat cattcagaat tctaaggtgt ctcctctcgt catcagcaaa tgacaggacc
     2101 atcttctctg ctgactcata tgtcaccttt gcgcttaggt cctcataaaa agtaatacca
     2161 tctttttcca tctggatagc ctgttcgata agtctcttta atttctcatc catcaataat
     2221 cacctctctt ttaaagtcct ttttcaaact ataccataaa gaccttataa ttacaaatct
     2281 ttcttagata ataaatccgg tatctttgcc agatcttctc tcgaaagttt taaccactct
     2341 ggatggtaaa ggcaggcagc cggatgaaat gttggcagaa ctactttatc acccttttta
     2401 aataccctac catgtacctt tgtcatacct aaatctgtct ctgccagtag tcttgttgaa
     2461 tgattcccca ttgttattat tactcttggg gatatataag atatctgggc aaaaaggtat
     2521 ggactgcagg cacttacctc atcaatattt gggtctctat tgtttggagg tctacatttt
     2581 ataacatttg ctatataaac tttgtctcta tttaatccga tctcttcaag gagtttatcc
     2641 agtaacttcc ctgccgagcc gacaaaggga agaccccgtt tatcttcata gtaccctgga
     2701 gcttccccca caataagtaa tttagcattg ggattaccat ccccaaagac caccctggtc
     2761 ctggttttat aaagatcgca cttcgtgcag gcttcaacct cttgtcttaa taactccagc
     2821 tcgttcatat atgatctccg gtatcttttc tatctcatta ctccttaaaa ctatactctt
     2881 ttcccttgag ctattccaca ggcaggttgc ctctttatct gtaaaatccc tgtcctctat
     2941 cggtggatca tctatcatta tggtaagctt gcctttctca atggcatacc caaaggattt
     3001 aagatttgcc aaattcccct ttccaaatgg taaagaagat ataactacca catctgacct
     3061 atctatcata tttaaattct tttggtagtt ctcatcagat atcggtgaga atggagcttc
     3121 aaatactgca ggaataccaa gttcattaca cacatctgca tcgctatcca gtagattcac
     3181 caccccacag gatagatcaa aatatctgtg caaaagatag agtatctttg cccccgtacc
     3241 cccaccacat attatgtgga cctttatatc caggtctcta taccctccgc tggatctaga
     3301 cagtggtagt atatatatac catcatatac tggatgctgt ctcactatga catctatatt
     3361 gaagacttct ttcaagttgg cgctggtcaa gacatccctg ggaggtccaa aatcatagat
     3421 cgtgccatct ttcatcagga taatcttatc acaaaattga gatgcaagat ttgggtcatg
     3481 gagggctgag agcacactta tacccttttc ctttacaagt ctttttataa gatcgagcat
     3541 ctctatctga taagagatat ccagatgtga tgtgggctca tccagaagca gtatctttgg
     3601 ctgctgagct agagcccttg ccagtattac tctctgtttt tctccaccgc tgagttccgt
     3661 tatgggtcta tcggcaaaat ctatagtatt ggttaacctc atagcctctc tcactatatc
     3721 ttcatcagac cccgtttctc ctcgaaacct ggagacataa gggtttcttc ccatcat
//
LOCUS       DAOTKU010000117         3754 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_20566_length_3754_cov_3.675588, whole genome shotgun sequence.
ACCESSION   DAOTKU010000117 DAOTKU010000000
VERSION     DAOTKU010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3754
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20566_length_3754_cov_3.675588"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1125)
                     /locus_tag="P5310_03800"
     CDS             complement(1..1125)
                     /locus_tag="P5310_03800"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRU32517.1"
                     /translation="MLLVANVSRDLYNFRIGLMRALKSEGFEVIAVAPEDNFSKRFIE
                     EDIRFIPINLKRGGRNPLTELILILKLYEIYKKEKPSLVIHYTIKPNIYGNIASRLAK
                     TKSISVVTGLGSLFVSKSVLQEMVSFLYRFSFKFCDRVFFLNSDDRNYFINRGIVQPE
                     KTVLIKGEGIDTEYFSKEKCLNIPEEDNNIVFLLISRMIRDKGIWEFVESARIVKRIY
                     PSTNFWLLGPIDKENPSGITIETIRGWEEKGLVSYLGVADDVRPLICKSDVVVLPSYR
                     EGIPQSLLEAMSMEKPIITTDSIGCRDVVEDGKNGFLVPVKNPEALADAMIKMIEMTK
                     EEREEMGKYGRNKVLREFDEKIIIRVYLEEIGKILSFQED"
     gene            1213..1314
                     /locus_tag="P5310_03805"
     CDS             1213..1314
                     /locus_tag="P5310_03805"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HRU32518.1"
                     /translation="MNRAFDWLKQAQRDLEQAKDSHKTGRYEWAYSR"
     gene            1329..1694
                     /locus_tag="P5310_03810"
     CDS             1329..1694
                     /locus_tag="P5310_03810"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HRU32519.1"
                     /translation="MPVRSLNSSILKWPNREKVEEELKKWIEEITQKRKDIIRIGYFG
                     SYARGDFGVGSDLDLIIILEDSSLPFERRGIEWDVITLPVPVDLLIYTLKEWEKLNEE
                     NSPFHKRVNKEVIWIFSRE"
     gene            complement(2158..2493)
                     /locus_tag="P5310_03815"
     CDS             complement(2158..2493)
                     /locus_tag="P5310_03815"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HRU32520.1"
                     /translation="MTDLSLWEEKLVREYEERELKRKKLLEEIIPKLREYFRDKKVDM
                     VFIFGSILEEGLFYNFSDVDIACGGLKENYFRVFSDLEDLVGRDIDLVELEYCKFREE
                     ILKRGIRIK"
     gene            complement(2563..2901)
                     /locus_tag="P5310_03820"
     CDS             complement(2563..2901)
                     /locus_tag="P5310_03820"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012895957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HRU32521.1"
                     /translation="MRVFYPKLNREEVVELLLQKFKILEKKLPIIRVVLFGSYAKGNY
                     TVGSDVDILVIYDGEKRNDDYAIVKKTLNIPRLEPHIYTKEEYEKIKDVLEKMTKDGI
                     LLFYKDLSFL"
     gene            complement(2873..3268)
                     /locus_tag="P5310_03825"
     CDS             complement(2873..3268)
                     /locus_tag="P5310_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HRU32522.1"
                     /translation="MERSQDWINEARWDLEHAKNDLATGFYNWACFSSQQSAEKAVKA
                     VFQKIGAEAWGHSVADLLAELSKRYQVSEELIGWALELDKAYIPARYPDAHPSGSPRN
                     RYTKEEARRLISYAEEIVRFCESLLPEIK"
     gene            complement(3506..>3754)
                     /locus_tag="P5310_03830"
     CDS             complement(3506..>3754)
                     /locus_tag="P5310_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012582968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32523.1"
                     /translation="WDDKVIINETKSTPRIDYINDFIDVLKEITDYFPEYRDKQIIPI
                     FSSLYLPEDIVDYLTRNKIYAMGMNDDTMDLLNFEKVK"
BASE COUNT         1151 a          813 c          536 g         1254 t
ORIGIN      
        1 ttaatcctcc tggaacgata agatcttccc aatttcttct aaatatactc tgataataat
       61 cttctcatca aattctctta aaaccttatt tctgccatac ttccccatct cttccctctc
      121 ctctttagtc atctctatca tctttatcat agcatcagct aaagcctctg gatttttaac
      181 cggaacaaga aatccatttt tcccatcttc cacaacatct ctgcatccga tggaatctgt
      241 ggtaattatt ggcttttcca tagacatagc ttctaaaagt gattggggta tcccttcacg
      301 ataggagggt aaaacaacca catcgctctt acagataagt ggacggacgt catctgctac
      361 tcccagataa ctgaccaagc ctttctcttc ccagcccctg atagtttcaa tggtgatacc
      421 tgaaggattt tctttatcta taggtcctaa taaccagaaa ttagtggaag gatatatcct
      481 ctttactatt cttgccgact ctacaaactc ccaaatgcct ttatcccgga tcattctgga
      541 tatgagaaga aacactatat tattatcttc ttcagggata tttaagcatt tctcctttga
      601 gaaatactca gtatcgatac cttcaccctt tattaatact gtcttctctg gctggactat
      661 tcctctatta ataaaatagt ttctatcatc actgttcaaa aagaataccc tatcacagaa
      721 cttaaatgag aatctgtata aaaaacttac catctcctgg agcacacttt tgctaacaaa
      781 aagagaccct aatccagtta ccacagagat tgattttgtt ttagctaatc ttgaggcaat
      841 atttccatat atattaggct taatagtata gtgtatcacc agagatggct tttccttttt
      901 ataaatctca taaagcttta agatcagtat cagttctgtt agtggatttc taccgcctct
      961 tttcagatta atagggataa atctgatgtc ctcttctata aaccttttac tgaagttatc
     1021 ctcaggggct acagcaataa cttcaaagcc ttcactcttt aatgccctca taagcccaat
     1081 tctaaaattg tacaggtcac gggatacatt ggcaactaaa agaattttcc tatttttcag
     1141 atatcaaaaa tcctctcaat tttaatttct ttcaattata ccataatttc gtatatctgt
     1201 tataatagat atatgaatag agcttttgat tggttaaaac aagctcagag ggatttagag
     1261 caggcaaaag attcccacaa aacaggaagg tatgaatggg catattcaag gtgaggaggc
     1321 tataaaatat gccagtgaga tccttgaatt cgtcaattct aaaatggcca aatcgtgaga
     1381 aagtagagga agaattaaaa aagtggatag aagaaataac acagaagaga aaagacatta
     1441 taagaatagg gtactttggg tcttatgccc gtggagattt cggtgtggga agtgaccttg
     1501 acctgataat aatcttagaa gattcatctc tcccatttga aagaagagga atagaatggg
     1561 atgttataac tcttccagtt cctgtagatc ttttgatata taccctgaaa gagtgggaaa
     1621 aacttaatga ggagaattct ccctttcata agagagtaaa taaagaagtt atatggatat
     1681 tctccagaga ataaatcttc ttttatactc cctgagaaga attcctttca gttatctcct
     1741 tataaaatct tacttaaact taactgttac tttcttcaaa actctctacc agctttatat
     1801 cccgtagggg caattcatga attgccccta caatctcgtt tttcatgata ataatcccat
     1861 atacttttac cgtcaaaaat gttagaatac atctgtacga actttgagga ataaaatttt
     1921 tagaggggaa ggcggaatga atcgatagac tcttttctaa gagaacttta taagcttgca
     1981 tctctcgata gagcaagcca gcttttcttc taatttagat actctggctt aaccagttct
     2041 taaattcatc taaatctctt ttaatatttc cccaatttgt aatgagctta tcctttaagt
     2101 ccttgacctt ttcaggaaaa atttcataag tgtaagcatg tctgatgctt tccttcctca
     2161 tttgattctt atacctctct ttaagatctc ctctctaaac ttacaatact ccaattccac
     2221 aagatcaata tcccttccca ccagatcttc tagatcagaa aatactctaa aatagttttc
     2281 tttcaatccc ccacaggcta tatctacatc agaaaaatta tagaatagac cctcttctaa
     2341 tatggaccca aagataaaaa ccatatccac ctttttatcc ctgaaatatt ccctaagctt
     2401 aggtataatt tcttccagaa gctttttcct ctttaattcc ctctcttcat attcccttac
     2461 aagtttttct tcccaaagag ataaatcggt cataaaaacc ccctaaatta tgctattccc
     2521 aaaaaccctc tacactctta tataaacgat accctgatta aactaaagaa aggataaatc
     2581 tttatagaat aacaatatcc catctttagt cattttctcc aaaacatctt ttatcttttc
     2641 gtactcttct tttgtataga tatgaggctc aagacgtgga atattaagag ttttctttac
     2701 tatagcatag tcatcatttc tcttctctcc atcataaatt acaaggatat ccacatcact
     2761 ccccacagta tagttccctt tagcgtaaga accgaaaaga actaccctga taattgggag
     2821 ctttttctca agtattttga atttttgaag aagcagttcg acaacttctt ctctatttaa
     2881 tttcgggtaa aagactctca cagaacctga caatctcttc tgcataagag ataagtcttc
     2941 tcgcctcctc cttagtatac ctatttcgtg gagacccgga aggatgagca tcaggatacc
     3001 ttgcaggaat atatgcctta tctaactcta aagcccaacc tataagttcc tctgagacct
     3061 ggtatctctt agataactca gctaacaggt cagctaccga atgtccccaa gcctcagctc
     3121 ctatcttctg aaagacagcc ttcactgcct tctctgcgga ctgctgagag gaaaaacatg
     3181 cccagttata gaatcctgtt gctaaatcat ttttagcatg ctctaaatcc catcttgcct
     3241 cgtttatcca gtcctggctt ctttccatag ttaaattata tcatcccttc gatagagtta
     3301 aaaacctttc catcacaccc tgattccctt atctataatt acctgcttta gactttcttt
     3361 gcaactctca atatctacaa gatctatctc aaagtcttca ctcatagagg tgacttcagc
     3421 tacggcttta aaatataagt tatcagggat tcccaatatc tttcagcttc ctctggggtt
     3481 aaatccttaa atgtctttga catagttact taaccttttc aaagttcaga aggtccattg
     3541 tgtcatcatt cattcccatg gcataaatct tattcctggt taagtaatct actatatctt
     3601 caggaagata tagggatgaa aatattggga ttatctgctt atccctgtac tcagggaaat
     3661 aatcggttat ctcctttaga acatctataa agtcattgat gtaatctatc cttggagtag
     3721 attttgtctc atttattatc actttatcgt ccca
//
LOCUS       DAOTKU010000118         3749 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_20598_length_3749_cov_2.943692, whole genome shotgun sequence.
ACCESSION   DAOTKU010000118 DAOTKU010000000
VERSION     DAOTKU010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3749
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20598_length_3749_cov_2.943692"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="P5310_03835"
     CDS             complement(<1..276)
                     /locus_tag="P5310_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HRU32524.1"
                     /translation="MIKLGVNSVLFGGFDFATACKYIALAGYDGVEISAIKGMCEHLE
                     LDRWQEQTDMVKSTVRDNGLEILSMEVASLDEDRLIKAFEASKELGNP"
     gene            complement(276..1274)
                     /locus_tag="P5310_03840"
     CDS             complement(276..1274)
                     /locus_tag="P5310_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016483090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRU32525.1"
                     /translation="MTLKVGIVGMGNIGNIHAGVYKENPKTEIVAVCDIIREKADRAA
                     EKYGAKAFYSVKEMLASGILLDACSMATAGKENGGDHFVPTMELLEGGIPVLGEKPIS
                     NDIECAKKMVALAKSKQIPYGINLNHRFTPAARRARQWMEDGRLGSINMINMRMWINN
                     PNESSPWFHLRALHPHSFDVMRYFCGDVKRVAAFMKKGEGRTIWSNTQILLEFKNGVI
                     GTLTGSYDAGGSYGLERCEVVGSKGRFVLEDACEHLHFYNRFSIETESYSYLGGMRSF
                     NETFKDRIGVWIDQIESGARYDEIEASGEDGLKAQLIIEAAIKSWETGSIVDVEEV"
     gene            complement(1421..2344)
                     /locus_tag="P5310_03845"
     CDS             complement(1421..2344)
                     /locus_tag="P5310_03845"
                     /inference="COORDINATES: protein motif:HMM:NF014212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aryldialkylphosphatase"
                     /protein_id="HRU32526.1"
                     /translation="MIMTVRGPVEDSNPGKTLTHEHVIIDFRGAEYTSNNDYEMSNVI
                     DIVYPFLEEIKDLGYKYLVDCTPQFFGRDVPVLRKLSELLDIHILTSTGCFAAGNDKH
                     IPSFFYSKDERGIAKIWVEEWKNGIDETGIKPGIIKVAVDKGPLSEIDNKIVSAACIT
                     YHETGLTIMCHTGEKECAMQVLEIIKKYKVPASKLIIAHADSIEDKNTIFKLADEGCY
                     VEYDWIGIKPIGYHVQLIKETIEKGFTAQILLSQDAGQYSVGEKDGGKEKFRPYTYVE
                     KQLIPELIKELDIRIIDKIQKVNPLYALSIQ"
     gene            complement(2405..3466)
                     /locus_tag="P5310_03850"
     CDS             complement(2405..3466)
                     /locus_tag="P5310_03850"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRU32527.1"
                     /translation="MDYSIQERLENYLRAINFKNPRWIPCRVSLMPATWRKYRGKLED
                     IVLSHPVIFPDYREGSIDFDAIKDPGYRKGGFTDSWGCVWENIEEGLDGMVVKFPLDD
                     WGKLDSYIPPDPIVQEERGGTPDWDKIRESLKERRSQGKLATGGLPHGSMYMRLFYLR
                     GFENLMIDIATDDPRLEKLISMVLDYNIKFINKWLELGVDLMYFGDDLGNQDRLPISP
                     EKFRKYLKPCYKKMFGLCREKNVYVYLHSDGHIIEIIDDLIECGVNILNPQVRANTVE
                     GLVKNAKGKVCIDLDLDRQLFPFATPEEIRRHIKDAVEKLNSPEGGLMLIAECEPDVP
                     LENIETICSVLEEVGGPSV"
     gene            3551..>3749
                     /locus_tag="P5310_03855"
     CDS             3551..>3749
                     /locus_tag="P5310_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011229210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRU32528.1"
                     /translation="MISKKKKISIIDVARVAGVSPATVSRVLNKTARVSPELSGRVIS
                     AVKELNYVPDPFAHGMRTKRTK"
BASE COUNT         1030 a          930 c          549 g         1240 t
ORIGIN      
        1 tggatttccc agttctttgc tggcttcgaa agcctttatc aatctatcct catctaaaga
       61 ggctacctcc atggagagaa tctccagacc attatctctt actgtacttt tgaccatatc
      121 ggtctgttcc tgccacctgt ccagttccag gtgctcacac atacctttta tagcagagat
      181 ttcaacccca tcgtagcctg ccagtgcgat atacttgcag gcggtagcaa aatcgaaccc
      241 gccaaataaa acagaattta ctcccagttt tatcattaaa cctcctccac atccacaata
      301 ctgccggttt cccaggactt tatagccgcc tctattataa gctgagcctt taatccgtcc
      361 tctcctgatg cctctatctc atcatacctt gctccagatt ctatctgatc tatccataca
      421 ccgatacggt ccttaaatgt ctcattgaaa gaacgcatac caccaagata actgtagctc
      481 tctgtctcga tactgaatct gttatagaaa tgcagatgct cacaggcatc ctccaggaca
      541 aaacgaccct tcgaacccac aacttcacat ctctccagac catagctacc accggcatca
      601 taactccctg taagtgttcc tatcacacca ttcttgaatt caagtaatat ctgagtattt
      661 gaccatatcg ttctaccttc accttttttc ataaaggcag ctaccctttt cacatctcca
      721 cagaagtatc tcatcacatc aaatgaatgg ggatgaagag ctcttaagtg aaaccagggg
      781 gaagattcat tcggattatt aatccacata cgcatattta tcatatttat gctaccgagt
      841 cttccgtctt ccatccactg tcttgccctg cgtgcagctg gagtaaatct atggtttaga
      901 ttaatcccat aagggatctg tttactctta gccagtgcta ccatcttttt agcacattct
      961 atgtcattag aaataggctt ttctccgaga acaggtatac ctccctctaa caattccatt
     1021 gtcggaacaa aatggtctcc cccattttct tttcctgcgg ttgccatact gcaggcatcc
     1081 aataatatgc cactggctaa catctctttt acactgtaga atgccttcgc cccgtatttt
     1141 tctgcagccc tatcagcttt ttctctgata atgtcacata ctgctacgat ctctgtcttt
     1201 ggattctcct tgtagacacc tgcatggata ttccctatgt ttcccattcc aactattcca
     1261 acctttagag tcatttcaga cctcctcttc tattttttga tgtaactata agattatatc
     1321 atcttatcaa taaatttcct tctaaatacc ccataatcat cattctgaat ttaaaaacta
     1381 caaaaacaga aatcttgcat actcttaatt atattagaag ctattgtatt gacagggcat
     1441 acaggggatt taccttttga attttatcta taattcttat atccaattct ttaattagtt
     1501 ctggaattaa ttgtttttcg acatatgtat agggacgaaa tttttcttta cctccatctt
     1561 tttcccccac actgtactgc ccagcatctt gagagagaag aatctgagcg gtaaatccct
     1621 tttcaattgt ttctttaata agctgaacat gatatcctat cggctttata ccgatccagt
     1681 cgtactctac atagcatccc tcatcagcta atttaaatat tgtattttta tcctcaatac
     1741 tatctgcgtg ggcaataatc aatttgctgg ctggtacttt atactttttt ataatttcaa
     1801 gtacctgcat tgcacattct ttttctcctg tatgacacat aattgttaag ccagtctcat
     1861 gataggtgat acatgcagca cttacaattt tattgtcaat ctcacttaga ggtcctttat
     1921 ctaccgctac cttaattatc ccaggcttta ttccagtttc atctatacca tttttccatt
     1981 cttcaaccca aatttttgct atcccccttt catccttact atagaaaaac gaaggtatat
     2041 gcttgtcatt gcctgcggca aaacatcctg tacttgtaag tatatgtata tccaaaagct
     2101 ctgataattt tcttaatacc gggacatctc ttccaaaaaa ctgaggggta cagtctacaa
     2161 gatatttata tcccaaatct tttatctcct ccagaaatgg ataaacaata tctatcacat
     2221 tagacatctc atagtcatta tttgaagtgt actctgctcc tctaaaatca ataattacat
     2281 gttcatgagt caaggtcttc ccaggattgg aatcttctac tggacccctt actgtcatta
     2341 tcatagtcta aatctctcct cgaaagtaga attaagggcg aggacaccat atttaaaatc
     2401 acttttatac cgatggtcct ccaacctctt caaggacact gcatatagtt tctatgttct
     2461 caagagggac atcgggttca cattcggcta taagcatcag ccctccttca gggctgttta
     2521 atttctccac agcatctttt atatgtctcc ttatctcctc tggagtagca aatgggaata
     2581 actgtctatc aaggtccaga tctatgcaca ccttaccttt tgcatttttt accagcccct
     2641 ctactgtgtt tgccctcacc tggggattta atatgttaac tccacactct ataaggtcat
     2701 ctatgatctc aatgatatgt ccatctgaat ggagatatac atatacattt ttctccctgc
     2761 ataaaccaaa catcttttta tagcagggct tcagatattt tctaaatttc tccgggctta
     2821 tcggtaatct atcctgattc cccaggtcat ctccaaaata catgaggtct acccccaact
     2881 caagccattt atttatgaac tttatattat agtccagaac catactgatc agcttttcca
     2941 gtctcggatc atcggtagct atatctatca ttagattctc aaaacctcta agatagaaca
     3001 gcctcatata catagagcca tgaggtaagc ctccagtagc tagtttaccc tgagatcttc
     3061 tctccttaag agactctctt atcttatccc agtctggcgt tcctcccctt tcctcctgaa
     3121 ctatcgggtc aggaggaata tacgaatcaa gttttcccca atcatcgagg gggaacttca
     3181 cgaccattcc atcaagacct tcctctatat tttcccagac acatccccag ctatcagtaa
     3241 atccaccctt cctgtaacca gggtctttta tagcatcaaa atctatactt ccctctctat
     3301 agtccggaaa tattacaggg tgactcagga ctatatcttc caattttccc ctgtactttc
     3361 tccatgttgc aggcataaga ctcaccctgc aaggaatcca tcgaggattt ttaaaattaa
     3421 tagctctaag ataattttcc aatctttcct gtatagaata atccattaat cttctcctcc
     3481 tttctttccc cttagtatat caaaatttat gttattatat tagtgtcatt aaaataaagg
     3541 agttttgcct atgatttcta aaaaaaagaa gataagtata atagatgttg ccagggtggc
     3601 aggtgtatct cctgctactg tatcccgtgt acttaataag accgcaaggg taagtcccga
     3661 gctttctggc agagttatct ccgctgtgaa ggagctgaac tatgtccctg atccttttgc
     3721 ccatggtatg aggacaaaaa ggacaaaaa
//
LOCUS       DAOTKU010000119         3738 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_20679_length_3738_cov_3.402389, whole genome shotgun sequence.
ACCESSION   DAOTKU010000119 DAOTKU010000000
VERSION     DAOTKU010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3738
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20679_length_3738_cov_3.402389"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="P5310_03860"
     CDS             <1..509
                     /locus_tag="P5310_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32529.1"
                     /translation="GQLPCSGSAQLGLLMVIFAIQMLASGSTPIGPFPRNWLMIGFGL
                     LFAALGIISSIIPGVMVKPLTIVIGLLNILGGIISLVKIVLPRLKKTGQPGGQVPPIL
                     QKLFITQLIMGLLSIMFGTSMLVSHLLPGLVVGVVLFANGCVLLYLMSILLTLDEMIS
                     QKLRGSTV"
     gene            918..2651
                     /locus_tag="P5310_03865"
     CDS             918..2651
                     /locus_tag="P5310_03865"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRU32530.1"
                     /translation="MKKSLISLLSVILVFVLVSSAIAAGQFNGAWPYSPPPVGHFNTY
                     ATNNISLGMYYDLMEQPLAMYLWATGKWMPLLATKWSLNGKTNTFRVYLRKGVKWQDG
                     KTFTAKDVLTTFYLGYLYNWAVWRYVDKVTAVDDYTVDFHMAVPCAPMVLERYILRTQ
                     IRSDAVYGKYASQVIALLNQGKKSDSDEMKKLRAEFDQFRPERIVGTGPFILDPKNLT
                     ESEATLTRFDGYWDSEKIQFDKIKVYNGETPVVTPLVLSKEVDYATHGFPPATEKQFI
                     QLGVKIIRPPTYSGPAIYFNHNIYPFNLKEVRQAIAYAIKKEENAFVSLGDSAKRQIY
                     MTGISDNLVPLWISEAVQKRLNKYDYNLDLAAKLLTNLGFKKGADGIWVTDKGTKMEY
                     ELLVPAEYADWSAAAENAAEQLTKFGIKTTVRGITYAQHPTEVRAGRFQMAIAAWGSA
                     HPHAHFSYYADLITYNYPSGLGPGMNFPLVQTTKSAGKVDLDKLITQSAEGYNTLEQK
                     KIVEKLALAFNELLPIVPLWERYGNNPALDGVRVTGWPKDSDPIYKNSPYADNAVAIM
                     LLNGTLKPVTK"
     gene            2726..3721
                     /locus_tag="P5310_03870"
     CDS             2726..3721
                     /locus_tag="P5310_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRU32531.1"
                     /translation="MSRVKRLLSNYIFIRVVRAILTVFIVATLTFFIVRLMPGNPIEI
                     YIGQLMSQYGMTFQDAKNQAAALFSLDLDAPLFEQYIIYMKNLLRGDLGMSFLSQGTP
                     VSLLIASYLPWTIFSVGISILISFTLGILLGMISAYKRGGIFDHIISTIAAILSAIPN
                     YLIGILLLIIIGVQLGLIPVSSMRGTVSPGITPGFNWTFIKDIFSHASLPILTYVVST
                     VGGWTLSMKSSTLNTISEDYVTVAKARGLPESRITLSYVGRNAILPLFTSLTMQMGFL
                     MGGSTLIESIFVYKGIGWALGSSIAQRDYPVMQAVFLIITIAVVVANFIAELLII"
BASE COUNT         1053 a          668 c          868 g         1149 t
ORIGIN      
        1 tggggcagct tccttgttca ggaagcgctc agcttggact gttaatggtc atctttgcca
       61 ttcaaatgct ggcatctggc agtacaccga ttggtccttt tccccggaac tggctgatga
      121 ttggatttgg acttttgttt gccgctttgg gaattatatc cagcattatc cctggggtta
      181 tggtaaaacc tttgactatt gttattggat tattgaatat ccttggcggt attataagtc
      241 tggtgaaaat agttttgcct cgtcttaaaa agacggggca accaggcggt caggttcctc
      301 cgattttaca aaagctattt attactcaat tgatcatggg cttactgtca attatgtttg
      361 gaacttcgat gctggtttct catctacttc ctgggctggt ggttggcgtg gttctttttg
      421 ccaatggatg tgtccttttg tatctgatgt ctattctcct gaccctggat gagatgataa
      481 gtcaaaagct acgagggtca acagtataag tagcgtataa gtagtataaa taattcgaga
      541 ataacctatt ttatcctccc ccatctcggg ggggataaaa taggaggctg ggagagggga
      601 atacttctga ctcgtagaag agatctatta tctttgtatt ctgtaggggc aatttatgaa
      661 ttatccctac aatcctgtta cgcgtcaccc atcacgcgtt acgtgttata cctgacaata
      721 tcacagagcg ttaacatgca tcttgaacat attctcacta atatgataaa atatagttgt
      781 gttaaatctt tatgaagaaa ggtggtgatc ttggagaaga tatctaaaag tttcataagg
      841 gttgtggtag tttccacaat taggtgaggg attttaggga gagcattaga gaaagagaat
      901 ttaagaagga gggatttatg aagaagagtt taattagttt gcttagtgtt atcttggtat
      961 ttgtcttagt ttcgtccgct attgcggcgg ggcagtttaa tggtgcctgg ccatattcac
     1021 caccgccagt tggacatttc aatacatatg ctaccaataa tatctcttta ggtatgtatt
     1081 acgatcttat ggagcagcct ttagccatgt atctttgggc tactggaaag tggatgcctc
     1141 ttcttgccac taaatggagt cttaacggaa agactaatac gtttagagtc tacctgagaa
     1201 aaggtgttaa atggcaggat ggtaagactt tcacagctaa agacgtgctt actacatttt
     1261 accttggtta tctttacaac tgggctgtct ggagatatgt ggataaggta accgcagtgg
     1321 atgactatac tgtagatttc catatggcag tcccctgtgc tcctatggtt ctggagagat
     1381 atatacttag gacgcagatt cgttctgatg cagtgtatgg aaaatatgcc agtcaggtga
     1441 ttgctcttct caatcaaggg aagaagagtg attccgatga gatgaaaaag cttagagcag
     1501 agtttgacca gtttagaccg gaaaggattg tggggacagg tccattcata ctggacccaa
     1561 agaatttaac tgaatctgaa gcaaccctta ccaggtttga tggatactgg gattcagaga
     1621 agattcaatt tgataaaatc aaggtctaca atggagaaac cccggtagta actccactgg
     1681 tcttaagtaa agaggtagat tatgcaaccc atggttttcc gcctgcaaca gaaaagcagt
     1741 ttatccagtt gggtgtaaag attataagac cacccacata ctctggtcct gcaatatact
     1801 ttaaccataa tatctatcca ttcaatctaa aagaagtgag gcaggcgata gcctatgcta
     1861 taaagaagga agagaacgct tttgtatccc tgggtgactc tgctaagagg cagatctata
     1921 tgaccggtat cagtgataac ttggttcctc tatggatttc cgaggcggta cagaagagac
     1981 tgaataagta tgactataat cttgatctgg cagcaaagct gttaaccaat cttggattca
     2041 agaagggtgc agatggtata tgggtgacag ataaaggaac aaagatggaa tatgaattgt
     2101 tagttccagc tgaatatgca gactggtcag cagctgcaga gaatgccgct gagcagttga
     2161 caaagtttgg aataaagacg actgttcgtg gtattaccta tgcacagcat cccacagagg
     2221 taagagcagg gagattccag atggctatag ctgcatgggg ttctgctcat ccgcatgctc
     2281 acttctctta ttatgctgac ctgattacat acaactatcc tagtggttta ggacccggga
     2341 tgaacttccc actggtccag accacgaagt ctgctggaaa agttgatcta gacaagctta
     2401 ttactcagag cgctgagggt tacaatacct tagagcagaa gaagatagtg gagaagttag
     2461 cccttgcatt taacgagtta ctccctattg tcccactctg ggagagatat gggaacaatc
     2521 cagctctgga tggggtaagg gtaacaggat ggcctaaaga tagtgatcca atatataaaa
     2581 atagtcccta tgcagataat gctgtagcaa taatgcttct taatgggaca ctaaagcctg
     2641 taaccaagta aatagtaacg gaattattta ggggcgcctg aaccttaggc gtccctaaag
     2701 tttatattac taggaggcaa gagacttgag tagggtaaag agacttcttt ctaactatat
     2761 atttatcagg gttgtaaggg caattttaac tgtatttata gttgcaacac ttacattttt
     2821 cattgtcaga cttatgccgg gaaatcctat agagatatat atagggcaac ttatgtctca
     2881 gtatggaatg acttttcagg acgcaaaaaa tcaggctgct gcgcttttct cccttgattt
     2941 agatgctcca cttttcgagc agtatatcat atatatgaag aatcttttaa gaggagatct
     3001 ggggatgtcc ttcttatctc aggggactcc agtttcacta ttaattgcgt cttatctgcc
     3061 ctggactatt ttcagtgtag ggatatctat tcttataagt tttactttag gaatcttatt
     3121 agggatgatc tctgcttata agaggggagg gatctttgat catattatat ctaccattgc
     3181 tgctatatta agtgccatac ctaattatct cataggaatt cttctcctta ttattatcgg
     3241 tgtccagttg gggctgattc ctgtaagttc gatgaggggg acagtctctc ccggaataac
     3301 tcctggattt aactggacat tcataaagga tatattttct catgcatcac ttcctatcct
     3361 tacatatgtg gtaagtacag ttggtggatg gacactgagt atgaagagca gtactctaaa
     3421 tactataagt gaggattatg taactgttgc aaaagccagg ggacttcctg agagtcgaat
     3481 cactctctcc tacgttggta gaaacgcaat tttacctctg tttactagtc tgaccatgca
     3541 gatgggattc cttatgggag gttctacact tatagagagt atatttgttt ataaaggtat
     3601 tggatgggca ttggggtcca gtatagccca gagggactat ccagtaatgc aggcggtatt
     3661 tttgataatt acaattgcag ttgtagtagc taattttata gcggagttac taattatctg
     3721 atctataagc ttacggct
//
LOCUS       DAOTKU010000120         3730 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_20732_length_3730_cov_3.584218, whole genome shotgun sequence.
ACCESSION   DAOTKU010000120 DAOTKU010000000
VERSION     DAOTKU010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3730
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20732_length_3730_cov_3.584218"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..883
                     /locus_tag="P5310_03875"
     CDS             <1..883
                     /locus_tag="P5310_03875"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HRU32532.1"
                     /translation="DYLFRSSASRLIFPGYQIVSGKEEEEDEIPLEKLDRDKPVELLE
                     LLPSQHFTQPPPRYTEASLIKLLEERGIGRPSTYATIVSTILDRNYVIIDDHFLKPTP
                     VGMTVSDLLTKHFPNIMDPNFTANMEKDLDEIEEGKEDWERLIVNFYQDFEKQVEEAK
                     EKAEINNILGNCPICGKPLVEKRSRFGIFIGCSGYPDCRYTRPVTRSTGVKCPKCGGD
                     IVGLKSKSGKYYYRCSNYPDCDFVAWGRPTSAKCPKCGYPLVTVRSKKGNSYRKCSNP
                     DCDYVLLGRRRSKSPKE"
     gene            887..2182
                     /gene="trmFO"
                     /locus_tag="P5310_03880"
     CDS             887..2182
                     /gene="trmFO"
                     /locus_tag="P5310_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HRU32533.1"
                     /translation="MRVTIVGGGLAGVESAYYLANRGIEVILYEMRPYISTEIHKTGY
                     LAELVCSNSLKSNVPYDASWLLKEEMRLLNSIVVETASVYSIPGGKSLVVDRELFSMG
                     ITERLLTNKNVYVIREECKDIPDDRPLIIATGPLTSEMFSNSLREKLGEDFLYFYDAT
                     SPVVTRESIDENECFWASRYNYGGADYLNCPVDEDTYNSFYDALVSAETVPLQDFEKD
                     IFYEACLPVEEIAKRGRDALRFGPFKPKGLIDPRTGKMPYAVLQLRREDSEGRLLSLV
                     GCQTRLRWKEQERVFRIIPALRNAEFVRFGVMHRNTFINSPTLLSPSLSLKLEPGIFF
                     AGQITGVEGYLESAATGIVAGINAYRLLIEKELVVFPDETAIGSLIRYITTHSSEDFQ
                     PMGINYGIMPELKHPSRKERRRIIAEMAIDSLRRFLNEL"
     gene            2172..3053
                     /locus_tag="P5310_03885"
     CDS             2172..3053
                     /locus_tag="P5310_03885"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HRU32534.1"
                     /translation="MSSEEYIDMFLRYMKSERGSSKNTLKAYQKDLVQFLNFIREDGI
                     EILSVSKDNIRDFISFLTEKRYNRNSIIRKLSTLRHFYSFMVKRGYTDKNPLVSLFSP
                     KIIKTLPGFLTQVEVEDLITSIPLDNPLGIRDRAIIECLYSTGVRIGELVQLDISNIK
                     EGNQVLIKGKGNKERIVFLGEYAIKSLKIYLEESRPKLLKNDREKALFLSKYGTRLTS
                     RSIERMMEKYGFLMGKDISPHTIRHSFATHLLENGADLRAVQELLGHSDLSTTQKYTH
                     ITLERLKEVYRKSHPRA"
     gene            3066..>3730
                     /locus_tag="P5310_03890"
     CDS             3066..>3730
                     /locus_tag="P5310_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014454046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRU32535.1"
                     /translation="MRIIVTGGAGFIASHVVDAFIKEGHTVGVVDNLSSGKRENINPN
                     AMFFEVDIRDREKLEEVFDIFKPDIIDHHAAQINVRYSVEDPVYDASVNIIGSLNLLE
                     LSRKYQVKRIIFASTGGAIYGDAGGLADESTPTAPISPYGASKRSVEIYLGYYNSVFG
                     LEYVALRYANVYGPRQDPFGEAGVVAIFSERILSGKPCVIYGDGEQTRDYVYVLDVVN
                     ANV"
BASE COUNT         1178 a          603 c          920 g         1029 t
ORIGIN      
        1 tgattatctc tttagatcaa gcgcatccag gcttatattc cctggctatc agattgtgag
       61 cggaaaggaa gaggaggaag atgagatacc tctggagaag ttagatagag ataagcctgt
      121 cgagttatta gaactcctac cctctcaaca ctttacacag ccacctccac gttatacgga
      181 ggcatctctt ataaaattac ttgaggagag aggaatagga cgtcccagta cgtatgccac
      241 tatagtgagc acaatattgg atagaaacta tgtaattatt gatgaccatt ttttaaagcc
      301 tacccctgta ggtatgacag tatctgacct tttgaccaag cattttccca atataatgga
      361 ccccaatttt accgccaata tggaaaaaga cctggatgag atagaagagg gtaaagagga
      421 ttgggaaaga ttgatagtga atttctatca ggattttgaa aaacaggtag aagaggcaaa
      481 ggaaaaggca gagataaata atatattggg aaattgtcct atctgtggaa agcctctggt
      541 ggagaagaga agcaggtttg gtatatttat tggatgttca gggtacccgg actgtagata
      601 taccagacca gttacaaggt ctaccggggt gaagtgccca aagtgtggag gggatattgt
      661 aggtttaaaa tctaaaagtg gtaagtatta ttatagatgt tctaattatc cagattgtga
      721 ttttgttgcc tggggtagac ccacaagtgc caagtgccca aagtgtggtt atccgctggt
      781 cacggtacgt tctaaaaagg ggaattctta caggaaatgc agtaatcctg actgtgacta
      841 tgtgctgctg ggtagaagaa ggagcaagtc ccctaaggag tagtctatga gggtgactat
      901 tgttggtggc ggattggcag gggtcgaatc ggcatattat ctggctaaca ggggtataga
      961 ggttatcctt tacgagatga gaccttacat ttctactgag atccacaaga caggatatct
     1021 tgcagaatta gtctgcagta attccctgaa atcaaatgtc ccatatgatg ccagctggct
     1081 attaaaagag gagatgagac tgctaaactc tatagttgtg gaaaccgcaa gtgtatactc
     1141 tatccctgga ggaaaatctc tggtggtcga tagagaactt ttcagtatgg gtattactga
     1201 aagattattg accaataaga atgtttacgt gatacgtgaa gagtgtaaag acataccgga
     1261 tgatcgacct cttatcatag ctacgggacc tctaactagc gaaatgttct ccaactctct
     1321 tagagaaaag cttggtgaag atttcctcta cttttacgat gccacaagcc ctgtagttac
     1381 cagggagtcc atagatgaga acgaatgctt ctgggcttcc agatacaact atggtggggc
     1441 agattatttg aactgtcctg tagacgaaga cacatataat tcattctatg atgcccttgt
     1501 atctgctgaa acggtacctc ttcaggattt tgagaaggac attttctatg aggcatgtct
     1561 tcctgtagaa gagatagcta aaagaggtag agatgctctg aggtttggac catttaagcc
     1621 caaaggtctc atagacccca ggaccggtaa gatgccatat gcggttttac agttaagaag
     1681 agaagacagt gagggaaggt tactgagcct ggtaggatgt cagaccaggc ttaggtggaa
     1741 agagcaggag agggtattca gaattatacc agcccttaga aatgccgaat ttgtcagatt
     1801 tggtgttatg catcgaaata cattcataaa ttctcccacc cttttatctc cttctttgag
     1861 cttaaagctt gaacccggaa tattctttgc agggcagata acaggtgttg agggatatct
     1921 ggagtctgca gctaccggta ttgtggcagg gataaatgcc tataggctgt tgatagagaa
     1981 agaactggtt gtatttccag atgagaccgc tattggaagt ttgataaggt atataacgac
     2041 ccatagttca gaagattttc agcctatggg tataaattat ggtataatgc cagagttgaa
     2101 acatccttcc cgtaaggaga gacggaggat tatagcagaa atggcgatag atagcctgag
     2161 gaggttccta aatgagctct gaagagtaca tagatatgtt tttaagatat atgaagtctg
     2221 agagaggatc aagtaaaaat acattgaagg cttatcagaa agatctggtt cagttcctca
     2281 attttatacg ggaagatggt attgaaatct tatctgtctc taaagataat ataagggatt
     2341 ttatatcatt ccttacagag aagaggtata atcggaattc gattataagg aagctttcca
     2401 cgttaaggca tttctatagt tttatggtaa agagaggata caccgataaa aatccgctgg
     2461 tcagtttatt ctctccaaaa attataaaga cactgccagg ttttcttacc caggtagaag
     2521 tggaggacct gataacgtct atccctcttg ataatcctct tggcataagg gatagagcta
     2581 taatagagtg tctatattcg acaggggtaa ggattggaga actggtccag cttgatatat
     2641 caaatattaa agagggaaat caggtactga taaaagggaa ggggaataaa gagcgtatag
     2701 tctttcttgg tgagtatgcc attaaaagtc tgaagatata tcttgaagag tcaagaccaa
     2761 agttactgaa aaatgataga gagaaggctc tatttttaag caagtatggg acgagactta
     2821 ccagtagaag tatagagaga atgatggaaa aatatggatt tctaatgggt aaagatataa
     2881 gtccacatac gataagacat tcatttgcga cccatctgct tgagaatggt gcagacctca
     2941 gggctgttca ggagctatta ggacattctg acctgtcaac tacacagaaa tatacacata
     3001 ttaccctgga aagattaaaa gaggtttata gaaagtcaca tcccagggct taaaaggagg
     3061 aaaatgtgag gataatagta acaggaggag caggatttat agcgtcacat gttgtagatg
     3121 catttataaa ggaggggcat accgttggag tagtagataa tctatccagt ggaaaaaggg
     3181 agaatataaa tccaaatgcg atgttctttg aggttgatat aagggataga gagaaattag
     3241 aggaggtctt tgatatcttt aagcctgata ttatagacca ccatgcggca cagataaatg
     3301 taagatactc tgtggaagac ccggtctatg atgcaagtgt gaatataatc ggttctctta
     3361 atctgctgga gttatccagg aaatatcagg ttaagaggat aatctttgcc tctactggtg
     3421 gagctatata tggtgacgct ggtggtctgg cagatgaatc tactccaacc gctcctatat
     3481 ctccctatgg ggcaagtaaa aggtcagtag aaatatacct cggatattac aattctgtat
     3541 ttggattaga gtatgttgcc ctgagatatg cgaatgtata tggtcccaga caggacccat
     3601 ttggtgaggc tggagtggta gcgatatttt ctgaaaggat actgtccggt aagccctgtg
     3661 tgatatatgg tgatggagaa cagaccagag attatgtgta tgtgctggat gtagtaaatg
     3721 caaatgtaaa
//
LOCUS       DAOTKU010000121         3718 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_20803_length_3718_cov_3.523069, whole genome shotgun sequence.
ACCESSION   DAOTKU010000121 DAOTKU010000000
VERSION     DAOTKU010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20803_length_3718_cov_3.523069"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1179)
                     /gene="miaB"
                     /locus_tag="P5310_03895"
     CDS             complement(<1..1179)
                     /gene="miaB"
                     /locus_tag="P5310_03895"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HRU32536.1"
                     /translation="MRFYIWTIGCQMNEKQTEELSSLLKMRGDIEVDSPESADIIILN
                     TCSVRRRAEVRIEGRIGEFKRLKEKNNPIIVVAGCYAQAHREKILERFPYVDKVVGPF
                     DFMNIPELLNDSGKLYTDEKRTDWKIILPERAKVSSFVRIMEGCNNFCSYCIVPYVRG
                     RERSKPSSMVIEEVAGLVERGAKEVVLLGQNVNSYGKDIPGEVSFAQLLYKLNDVEGL
                     ERIRFTTSHPKDMSDELIEAMAELPKVCEHLHLPVQGGNDRILKLMNRGYTKAQYRAL
                     VDKIRDKIPGIALTTDIIVGYPTETEEEFQELLEFIREIRFDQVYSAIFSRRPMAKAS
                     ELPDLPYEIKQKRYEIFNQVQNEISREINEKFIGTIQEVLIEEAKTKFPDQPKGRTRT
                     N"
     gene            complement(1176..3179)
                     /gene="glyS"
                     /locus_tag="P5310_03900"
     CDS             complement(1176..3179)
                     /gene="glyS"
                     /locus_tag="P5310_03900"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HRU32537.1"
                     /translation="MRTFLLEIGTEELPARIFPDILAQMGDKARGLLDKYRVNYKDIE
                     VFATPRRLLVFIYGLDETQRDEIRKVKGPPRSVSLDNNGNPTRALIGFAERQGCSIED
                     IKFESTPQGDYAFAYIKEGGRPVREILGELSGELITSLEFQRSMYWEETKFLFARPVR
                     WILSILGDEVVPFTIAGIDSNRYTFGHRFLSPGVIEIKDVNRYKDMLESAYVMFDHKK
                     RREYILEKVGEKSREISANPILTDELLDEITFLVEYPYIILGEFSGDFLQIPREILTT
                     VMIHHQRYIPLEKDGVLYPGFIIVSNMDGRAEENIKKGNERVISARFSDAKFFYTRDT
                     AIPLGERVKDLEKISLPGERGTLRDKVDKMRLVLNYIIDTLGISHMRDDLNVALTLCE
                     ADRTTEMVKEFPELHGVIGGIYASLNEKEEIWKSIYHHEDENPPLFGAKLIAIAERMY
                     DIVSHFNEGYIPSGSQDPYGLRKSAYSLVSILGEGNIDIELGPIMDILECKNKKDVWD
                     FISQRLFNYLLAKGLKRDLVESAIGLDSTSIARITETAVTLQELSARESFPHFITSAV
                     RCFRITRDLNNLPECDPRYFQEEDERILYDFVVNFPQNENLVLAERYERLLPLVDILD
                     RFFKNVFVMVDDERIKNNRLSLLKETYKLFSPFGDLTRVEERL"
     gene            complement(3176..>3718)
                     /locus_tag="P5310_03905"
     CDS             complement(3176..>3718)
                     /locus_tag="P5310_03905"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014904722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HRU32538.1"
                     /translation="MLHDIRFVEDNWESPTLGAWGLGWEVWLDGMEITQFTYFQQMGG
                     LPVDVVSAEITYGLERIAMYVQEKDNVFDLLWNEDITYGEVRFQEEVEFSKYALLNGD
                     NKTLRDFFDAYEREAKRLLDISLILPAYDYTLKCSHIFNLLDAGGMLSYTDRVSYIGR
                     VRALSRDCAKLYLEMKGVTV"
BASE COUNT         1003 a          918 c          629 g         1168 t
ORIGIN      
        1 atttgttctt gtcctcccct tgggctgatc gggaaatttg gttttagcct cttctatcag
       61 gacctcctga atagttccaa tgaacttttc atttatctct ctggatattt cattctgaac
      121 ctgattgaat atctcgtatc ttttctgctt tatctcgtat ggaaggtctg gtaattctga
      181 tgccttagcc ataggtcttc ttgaaaagat agcagaatag acctggtcaa atcttatctc
      241 tcttataaat tccagaagct cctgaaattc ttcctctgtc tcggtaggat aaccgacaat
      301 tatatctgta gtgagcgcta tgcctggtat cttatcccta atcttatcaa ccagtgctct
      361 atactgtgcc tttgtatatc ctctattcat aagctttaag attctgtcgt ttccaccctg
      421 gacgggaagg tgtaaatgct cacacacctt agggagttca gccatagctt ctataagctc
      481 atcagacata tcttttggat gagatgtggt aaaccttatc ctttcaagcc cctctacatc
      541 attgagttta taaagaagtt gagcaaaaga gacctctcca gggatatctt taccgtatga
      601 attaacattc tgtccaagca aaacaacctc tttagctcct ctttcaacaa gccctgctac
      661 ttcttcgata accatactgc tcggcttact cctctccctt cctctcacat atggcactat
      721 acagtagcta cagaaattat tgcaaccctc catgattctt acaaatgagg agacctttgc
      781 cctctcaggg agaattatct tccagtctgt ccttttctcg tcagtataaa gtttaccgga
      841 atcgttcaac aattcaggta tattcataaa gtcaaatgga ccaacaacct tatcaacata
      901 tgggaatctt tcaagtatct tttctctatg agcctgcgca taacaaccgg ctaccactat
      961 tatagggtta ttcttctctt tcaacctttt aaactctccg attcttccct cgatacggac
     1021 ctctgcacgt ctcctcaccg aacaggtgtt taaaatgata atgtcagcag attcaggaga
     1081 atccacctct atatctcctc tcatcttcag gagagaagaa agttcttccg tctgtttttc
     1141 attcatctga catccgatag tccagatata aaacctcata acctttcttc taccctggtg
     1201 agatctccga atggagaaaa tagtttatag gtctctttaa ggagtgataa cctgttattt
     1261 tttatcctct cgtcatcaac cattacaaag acgttcttga agaatctatc aagtatatcg
     1321 accagcggta agagcctctc atatctctca gcaagaacta agttctcatt ttgaggaaaa
     1381 ttcaccacaa agtcatataa aatccgctca tcctcttcct gaaaatatct ggggtcacac
     1441 tctggaagat tattcaggtc tctggttatc ctgaaacatc ttacagcaga ggttataaaa
     1501 tgagggaaag actctctggc agacagctct tgaagtgtaa ctgcagtctc agtaatcctg
     1561 gcgatagagg tgctatcaag tccgattgca gattcaacca ggtctctctt tagaccttta
     1621 gcaaggaggt agttgaatag cctctgggat ataaaatccc agacatcttt tttatttttg
     1681 cattctagta tatccataat tggaccaagt tctatatcta tattaccctc tcccagtatg
     1741 gatacaagac tgtaggctga tttcctcagt ccataagggt cctgtgatcc ggaagggatg
     1801 tagccctcgt taaaatggga cactatgtca tacatcctct cagctatagc tataagcttg
     1861 gcaccgaata gaggagggtt ctcatcctca tgatgatata tcgatttcca tatctcctct
     1921 ttttcattaa gacttgcata tataccacct ataaccccat gcagctcggg aaattctttc
     1981 accatctctg ttgtcctgtc agcctcacag agagtgagag cgacattcaa atcatctctc
     2041 atatgagaaa tcccaagggt atctattata tagtttaaga ccagcctcat tttatccacc
     2101 ttatctctaa gggtacccct ctctccgggg aggcttatct tttccagatc ttttaccctc
     2161 tccccaagag gtatggctgt atctctggta tagaagaact ttgcatcgct gaatctggca
     2221 gatatcaccc tctcgttacc cttttttata ttctcctcag cccttccatc catattactc
     2281 actatgataa atcctggata gagtacgcca tctttttcca atgggatata cctctgatgg
     2341 tgtatcataa cggtcgtaag gatttccctg ggaatctgga gaaaatctcc agaaaactca
     2401 ccgagtatta tatatggata ttcgacaagg aatgttatct catctaaaag ttcatcggtt
     2461 agtatggggt tagccgaaat ctccctggat ttctccccga ccttctctaa tatatattct
     2521 cttctctttt tatggtcgaa cataacataa gcagactcca gcatatcttt atatctgttt
     2581 acatctttta tctctataac gccagggctt aaaaatctgt gcccaaatgt atacctgtta
     2641 gaatctattc cagctatagt aaaaggaaca acctcatccc caaggatgga gagtatccac
     2701 cttacaggtc tggcaaagag gaattttgtc tcttcccagt acatactcct ctgaaactca
     2761 agagaagtta taagctctcc tgacagctcc ccaaggattt ctcttaccgg tcttccccct
     2821 tcctttatat atgcgaatgc ataatccccc tggggtgtgc tttcaaattt tatatcttct
     2881 atactgcatc cctgtctctc ggcaaacccg atgagagctc tggttggatt tccattatta
     2941 tccagtgaga cactcctggg aggacccttg acttttctta tctcatccct ctgagtctca
     3001 tctaaaccat agataaacac taagagtctt ctcggcgttg caaagacctc tatatcttta
     3061 taattgaccc tgtatttatc cagcaatcct ctggctttat cccccatctg agccagaata
     3121 tctgggaata ttctggctgg aagttcttct gtccctattt caagaaggaa ggtcctcata
     3181 ccgttactcc tttcatctct agatatagct tggcacaatc tctggagagg gctctcactc
     3241 tccctatgta agatacccta tcggtatatg agagcatacc cccagcatcg agtagattaa
     3301 atatatgaga acacttaagg gtataatcat aggcggggag gatgagagaa atatcaagca
     3361 atcttttagc ctctctctca taggcatcaa aaaaatctct caatgtctta ttatcaccat
     3421 ttaacagggc atattttgag aactccacct cttcctgaaa tctgacttca ccataagtga
     3481 tatcttcatt ccagaggagg tcaaagacat tatccttctc ctggacatac attgcaatcc
     3541 tctccagacc ataagttatc tcagcggaga ccacatcaac cggtaatccc cccatctgtt
     3601 gaaaatatgt gaactgggta atctccatcc catcgagcca gacctcccag cccaatcccc
     3661 aggcgccgag ggtgggtgac tcccagttat cctcaacaaa tcttatatcg tgtaacat
//
LOCUS       DAOTKU010000122         3703 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_20892_length_3703_cov_5.455044, whole genome shotgun sequence.
ACCESSION   DAOTKU010000122 DAOTKU010000000
VERSION     DAOTKU010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3703
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20892_length_3703_cov_5.455044"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..351)
                     /locus_tag="P5310_03910"
     CDS             complement(115..351)
                     /locus_tag="P5310_03910"
                     /inference="COORDINATES: protein motif:HMM:NF041551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HRU32539.1"
                     /translation="MSRVTQLITLSIPPEMYEKIRELMKKENRTRSELIREAIRRYIE
                     EKEWEEIYRYGEKKAIEKGITEDQVEDIIDARRK"
     gene            524..847
                     /locus_tag="P5310_03915"
     CDS             524..847
                     /locus_tag="P5310_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32540.1"
                     /translation="MLLSIIQNLPLHKKSKEVLAIIVMSLIETEIPSLRGMAKKAINK
                     KFVDPERYSFKESLVNRIRRSFNLHKFDYIKAQLALAEFILNRVNRRRINIHVLTDFT
                     YLKDD"
     gene            complement(1015..1326)
                     /locus_tag="P5310_03920"
     CDS             complement(1015..1326)
                     /locus_tag="P5310_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32541.1"
                     /translation="MQLNIPDSAVKAEVIFHVGAHYLLDDNGDAVDQNDGIEALLKNV
                     YLGTNKVTGWEVASYDNLGVYQYIGTIEEDETLRGRIVGGEMMLVDQGFVTFSAEVPL
                     T"
     gene            2294..2704
                     /locus_tag="P5310_03925"
     CDS             2294..2704
                     /locus_tag="P5310_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32542.1"
                     /translation="MTLKEACKRIGVNYNTAKTYISELKKSGLTIGRKIGNRLDISEE
                     ELRILKEISETSVKEFLKKNKVMDIEPVKNEKDKTELQLLSKFTEEIERQNNEIKRLN
                     EELDLLKERLLKLESEVSNKDTFWTRLKKFLFPG"
     gene            3067..3669
                     /locus_tag="P5310_03930"
     CDS             3067..3669
                     /locus_tag="P5310_03930"
                     /inference="COORDINATES: protein motif:HMM:NF025934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047487 - oligogalacturonide lyase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligogalacturonate lyase family protein"
                     /protein_id="HRU32543.1"
                     /translation="MSSGHGKSFHIEFEKQKDKDTGVIVTRLTDGMGNSIHPYFTQPL
                     VSNDSRILLIESTRSGRWQLFSLELDTGLMVQLTDDPGIRPHSSCLDPNRLIAYYWDG
                     KILKSVDLNTLKTDQLYFVPSGFHTGILSLTADGRYLAFVYSEDLEISTGTGAQYSEM
                     LEHLYRRPSSVIMRIDLESQRIEAAWGEREWISHVTSSHH"
BASE COUNT         1183 a          703 c          692 g         1125 t
ORIGIN      
        1 ggagagatat aaacttcgat ctttttctcc cagattaaat ctaaaacttc tctgggagtt
       61 cctctaaaat ttaatccaga gatgaagacg tttgtatcta taactaccct gagtttattt
      121 tcttcttgca tcgattatat cttcgacctg atcctctgta atcccctttt ctatagcctt
      181 cttttctcca tagcggtaaa tctcttccca ctctttctct tcaatatatc ttcttatagc
      241 ctctcttatc agttcactcc ttgttctatt ttcttttttc attaattccc tgatcttttc
      301 atacatctca ggaggtatag agagggttat taattgagtt actctggaca tatagcctcc
      361 tttaatacta agtattaata agattatgat atcaaaagat ggaagggtct gtcaagattg
      421 tgctattaac tggtctgtaa tcactttctt gaaaagaaac tcctttccgg ctagaattat
      481 attcaatcat tactaaatcc taaccagaaa ggagtgattc tttatgttat tgagtattat
      541 acaaaattta ccactgcata aaaagagtaa agaggtttta gctataatag tgatgtctct
      601 tatagaaact gaaatcccct ctttaagggg aatggctaag aaagctataa ataaaaagtt
      661 tgttgaccca gaaagatact catttaaaga atctctagtt aacagaataa gaagatcctt
      721 caatctccat aagtttgact acatcaaagc ccaattagct ttagctgaat ttatcctaaa
      781 tagagtaaat agaagacgta taaacattca tgttctcaca gatttcactt atttaaaaga
      841 tgattgaaga atcttaaatt ttgctattcc tgtcggaggg tatctaaaag tgattacgag
      901 gggggctttc ccgtcgggag gtcgtaagca accctctccc cttctattgt tcctgatgga
      961 tatcctccaa cacaagagta gaggggagaa gaaacttctc ccctctactg gatgctacgt
     1021 tagaggaact tctgcggaaa atgtaacaaa accctgatct acaagcatca tttctccacc
     1081 aacaatccta cccctaagcg tttcatcctc ttctatggta cctatatact gatatacccc
     1141 taaattatca taactagcta cttcccatcc ggtaacttta ttagtcccta ggtaaacatt
     1201 ctttaagagt gcctctatac catcattctg atcaaccgca tcaccgttat catcaagcag
     1261 ataatgtgcc cctacgtgga aaatgacctc agcttttact gcgctatcag gtatattaag
     1321 ttgcacagaa tctctcctaa agaaatttac agcactggct ggagggaact gttctgaaag
     1381 ctctccaaaa atagtatccc cccttatcac ttttaccaga tcgttcctaa ctttgccaat
     1441 ttcagcatcc tgggagtcat agtagtgtac ctctatatag caaagaaggg caactctgtc
     1501 attttcattg tctgcagtgc agtatggtac cttatagtca tatgaaagtg aagaaggact
     1561 gggaccacac tgacatccag aagtccctgc ctatccagta gcaataagac caagaagtac
     1621 taagagtata ccaagagaaa tcttttttct aatcacttca atcgcctcct aatatatttt
     1681 gtgatacttc taaaactcta aaactctcta caaccttacc tctctttata tgttcacctc
     1741 ctttccaaac tatccctaac atattatatt tttaaagctc atctaaagtt cgtcggatca
     1801 taaagcaatt gaaatcatgt atttttgtca cattaatagt acaatcttga gagtttcttc
     1861 catctgaagt tttagattta atctgggaga aaaagatcga agtttatatc cctcctttaa
     1921 tgggtaacaa gtgaaacatg acgcttgatg gacgaaatat ccccctctcc cttgagggag
     1981 agggcagggg tgagggtgaa atttaaacat atattaataa aaaaatcggt tgagaaggtt
     2041 ggttgtgagt tggttgaggc gtatcaaatc ttttctaatc aatatatatt taactcaacc
     2101 aagcgattgg ttgggttgag ttggttgtga taaatattat ttataattta atatcttgtt
     2161 ggttggttga gttggttgta agttggttga gatgggtata actatcttat agaaatactt
     2221 aattgcttca accgacttgt tggttgagga aggttggttg acaggaaata aacttagtga
     2281 taggcttatt tgtatgacac ttaaggaagc ctgtaagagg attggagtaa attataacac
     2341 tgccaagact tatatatctg agttaaagaa atcaggatta actattggca ggaaaatagg
     2401 taacaggtta gacatatctg aagaagaact caggattctg aaagagatat ctgagacatc
     2461 cgtaaaagag tttctcaaga agaacaaagt gatggatata gaaccagtaa aaaatgagaa
     2521 agataaaact gagctccagt tactcagcaa atttactgaa gagatagaga gacaaaataa
     2581 cgagataaaa agactcaacg aagagttaga cctgttaaaa gaaaggttat taaagctaga
     2641 atccgaagtt tctaacaagg acactttctg gaccaggcta aagaaatttt tgtttccagg
     2701 ttaaccctga caaccggaat cttccaccag catttttctc gatcagactg ttttacagaa
     2761 tgcccaaaat atccccaaaa gaagcccgat aaggggggtt ggggggtaaa gtaatataaa
     2821 ttatcgtaac gcgtagtaaa tgacgcgtag cagatgtttt ttgcccatta cgcgtcactt
     2881 attccatgcc tctttgcttt agattaatat tctgttattt gatgaagata gcaaaggtaa
     2941 tctgataagt agtgagaaca tacacttaca cgtcaccatt atcacagaac gatgacaaat
     3001 ccttgacaat tctttattgg ggaatagaat tatagataaa ttcataaaag aggaggttat
     3061 aaaaatatgt cttctggaca tgggaaaagc tttcatatag agtttgaaaa acagaaggat
     3121 aaagatacag gggttatagt taccagactt accgatggga tgggaaacag tatacatcca
     3181 tactttaccc agcctcttgt ctcgaatgat tcaaggatac tcctgataga atctaccagg
     3241 agcgggagat ggcaattatt cagcctcgaa cttgacacag gtcttatggt ccagcttaca
     3301 gatgatcctg gcatcagacc acacagttcc tgcctggatc cgaatagact tattgcctat
     3361 tactgggatg ggaaaatatt aaagtctgtc gaccttaata ccttaaagac tgaccagctt
     3421 tattttgttc cttcaggatt ccatacggga atcctctctc taacagctga tggaagatat
     3481 ctggcatttg tctattctga agatctggag ataagtacag gaacaggggc acagtattca
     3541 gagatgctgg agcatctata caggagacct tcaagtgtga taatgagaat tgatctggaa
     3601 tcccagagga tagaagcagc ctggggagaa agagaatgga taagccacgt gacatcctcc
     3661 caccactaaa gtggtgggct tcctccttca ccgaggtgac tta
//
LOCUS       DAOTKU010000123         3697 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_20942_length_3697_cov_3.132619, whole genome shotgun sequence.
ACCESSION   DAOTKU010000123 DAOTKU010000000
VERSION     DAOTKU010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3697
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20942_length_3697_cov_3.132619"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1780
                     /locus_tag="P5310_03935"
     CDS             <1..1780
                     /locus_tag="P5310_03935"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HRU32544.1"
                     /translation="SVALCLTSAPFNGPIGSVRVGLVDGEFVFNPLSSQLEEGELDLL
                     VVGTRENIVMIETSAKEIPEDKYIEALHKAIGYIRENISIQEEFIDMAKGEVTPVNAT
                     IIQPDPELVDFIKRTTYDRINEILRIGKKKERESRMSKEPSELIEKAFQEMGEEGLAL
                     KEKFDSNPVMVKMIFKAMEREILERMVLDERIRADNRKFNEVRPISCEVGVLPRTHGS
                     AIFSRGETQVLTVVTLGSKGEEQIIDDLGIEESKRYMHHYNFPPYSVGEIRPLRGPGR
                     REIGHGALAEKALVPVIPEEDAFPYTIRVVSDVLESNGSTSMASTCGSTLALMDAGVP
                     IKAPVAGIAIGLVSRSDDEWVTLTDIQGLEDAYGEMDFKVAGTERGITAVQLDTKRFN
                     GVPIYILEKALEQAKEAREMILERITETIPEPRKTLSPYAPRIISLNVSPDKIGEIIG
                     PGGKVIKKIIEETDAKIDIEQDGRIYIYSQDEASGNVARNRIEEIAKEVEAGQVYEGK
                     ITRVTDYGLFVEVLPGKEGLLHVSQMERLKAPINKVFKPGDSVLVKIYEIDNLGRINL
                     TRKGLFPPEKSEKEDTGRFGNQRRKQ"
     gene            1816..3012
                     /locus_tag="P5310_03940"
     CDS             1816..3012
                     /locus_tag="P5310_03940"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRU32545.1"
                     /translation="MGFWYFTGSRIEREGEKGYSHLIEHLIFQGTEDKDAKAIALAFD
                     RLGTEANAFTERDSTCFYVKVLKKYALPAVDLLAQMLSYPAFRKKDVEKEKKVVIEEF
                     KSYNDSPEQKIHDMGLKSIWGDHPLSYSPLGKVDDLLDADPDKLRSFWEENYTSDNLL
                     IAITGDIDREDVMNALSKYPLLNRYRKTLRDLSSPSVNPTLISEDDDTEITHIAIAGP
                     GVSYMEPYLYAANALTIILSNSMSARLFQKIREEKGLAYSIYSYLLPFKDAGIFVSYV
                     GTGSNSNEEVLSIILDEYKALCNEPVTEEELDIAKAHLASSMILGTEGTFGRMEYIAR
                     TFYSYGKIISLDEVLNKIQVITPEDIQEISPRLFKDKLGIAVLGKNGRKIGENLWEIL
                     KSKLKD"
     gene            2988..3419
                     /gene="dut"
                     /locus_tag="P5310_03945"
     CDS             2988..3419
                     /gene="dut"
                     /locus_tag="P5310_03945"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012513193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HRU32546.1"
                     /translation="MKIQVKRLKDNTALPAYMTEGSSGLDLYSAEEKIIPPGKWEVIS
                     TGIAVEIPYGYEGEIRSRSGIAKDHGVFVLNSPGTVDSDYRGEVRVILMNLGDKSFNI
                     NTGDRIAQLVISPVVQVEVMEVDELSDTGRNAGGFGHTGRR"
     gene            3423..>3697
                     /locus_tag="P5310_03950"
     CDS             3423..>3697
                     /locus_tag="P5310_03950"
                     /inference="COORDINATES: protein motif:HMM:NF013293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32547.1"
                     /translation="MVRVMVCGAYGRMGQEVIKALSEQKDMMIVGAIDVRGVGESVFG
                     ITVRDNLEEVIKETRPSAIVDFTKREAALEDIKTGVNGQDLVGLVFHT"
BASE COUNT         1172 a          577 c          956 g          992 t
ORIGIN      
        1 ttctgttgct ctatgtctta catctgctcc atttaatggt cccatagggt cggtaagggt
       61 aggattggtt gatggagaat ttgtatttaa tcccctttct tctcagctag aagagggtga
      121 gctggacctt cttgttgttg gcacaagaga aaacatagtg atgatagaga catcagccaa
      181 agaaatcccg gaggataaat atatagaagc tctgcataaa gccatagggt acataagaga
      241 gaacatatct attcaggaag agtttataga tatggctaaa ggagaggtca ctccggttaa
      301 tgctaccatc atacaacctg atcccgagct ggtagatttt ataaagagga ccacctatga
      361 taggattaac gagatactta gaataggtaa aaagaaagaa agagagtcca ggatgtcgaa
      421 agagccttca gagcttatag agaaggcttt ccaggagatg ggagaggaag gactggcgtt
      481 aaaagagaag ttcgattcta atcctgtgat ggttaagatg atattcaagg ctatggaaag
      541 agagattctg gaacggatgg ttctcgatga acgtatccgg gctgataaca ggaagtttaa
      601 tgaggtcaga cctataagct gtgaggttgg ggtattaccc aggactcatg gtagtgctat
      661 attttcaagg ggagaaactc aggtccttac agttgtaact cttggctcca aaggtgaaga
      721 gcagattata gatgaccttg gtatagaaga gagtaaaagg tatatgcatc attataattt
      781 cccaccgtat tctgttgggg agatcagacc acttagaggt ccaggtagaa gagagattgg
      841 acatggagct ttagcggaaa aggctctggt accggttata ccggaagaag atgccttccc
      901 ctatactata agagttgtct ctgatgtgct ggagtctaat ggttctactt cgatggccag
      961 tacctgtggc agtacacttg cccttatgga tgcgggagtc cctattaaag caccagtcgc
     1021 aggcatagcg attggacttg tgtccagaag tgatgatgag tgggttactc ttacagatat
     1081 acagggattg gaagatgctt atggagagat ggattttaaa gtggcaggaa ccgaaagggg
     1141 tataactgcc gtacagcttg ataccaagag atttaatggg gttccaatat atatccttga
     1201 gaaagctctg gagcaggcta aagaagccag agagatgata ctggagagga ttacagagac
     1261 tatccctgaa ccaaggaaga ccctctcccc atacgcccca aggattatct ctcttaatgt
     1321 ctcaccagat aagataggag agattatagg acctggggga aaagtcataa agaagattat
     1381 tgaagaaaca gatgcaaaga ttgatataga gcaggatgga aggatatata tttattctca
     1441 agatgaagcc tctggcaatg ttgcccgcaa caggatagaa gagattgcca aagaggtgga
     1501 ggctggtcag gtatatgaag gaaagattac cagagttact gactatggtc tttttgtaga
     1561 ggttctgcca ggtaaagaag gattgttaca tgtctctcag atggaaaggt taaaggctcc
     1621 gattaataaa gtgtttaagc ccggggattc tgtcctggta aagatctatg agatagataa
     1681 ccttggaaga ataaacctta ccagaaaagg tttattccct cctgaaaaat ctgaaaaaga
     1741 agatactggg aggttcggga atcaacgtcg gaaacagtaa tcctggatag aattcagggg
     1801 gcaaaatctg tcagtattgg tttctggtat tttactggtt ccaggataga aagagagggg
     1861 gaaaagggct actctcatct tatagaacat ttgatatttc aagggaccga agataaagat
     1921 gctaaggcta tagctttagc gtttgataga ttggggacag aggcaaatgc ttttacagag
     1981 cgagacagta cctgctttta tgtcaaggtg ttgaagaaat atgctctacc tgcagtggat
     2041 ttgctggctc agatgttgag ctatcctgcc ttcaggaaaa aggatgtgga gaaagagaag
     2101 aaagtagtta ttgaggagtt taagtcctac aatgatagtc ccgagcagaa gatacatgat
     2161 atgggactta aatctatatg gggagaccat cccctgagct attctccgct aggaaaggta
     2221 gatgacctct tagatgccga tcctgataaa ttgcggtcct tctgggaaga gaattataca
     2281 tcagataatt tattgattgc cataacgggg gatattgata gagaagacgt gatgaatgca
     2341 ctctctaaat atcctctgtt aaatagatac agaaagactt taagggattt atcatctcct
     2401 tctgtaaatc caacccttat aagcgaagat gatgatacgg agattactca tatagctatt
     2461 gctggaccag gtgtttctta tatggaacca tacctctatg cagccaatgc attgactata
     2521 atactatcta atagtatgtc tgccagactc tttcaaaaaa taagagagga gaaagggctg
     2581 gcttattcta tatattctta tcttttaccc ttcaaagatg caggtatatt tgtctcttat
     2641 gtggggacag gaagtaactc aaacgaagag gtgctcagca tcatactgga tgaatataaa
     2701 gctctctgta atgaacctgt aactgaagaa gaattagata tagccaaggc tcatctcgct
     2761 tcgagtatga ttctgggtac agagggaacg tttggcagaa tggaatatat agcaaggact
     2821 ttttattctt acggaaagat tatttctctg gatgaagtcc tgaataaaat tcaagttatt
     2881 actcctgaag atatacagga gatttccccc agattattta aggataaact tgggatagca
     2941 gtcctgggga aaaatggtag aaagattgga gaaaatctat gggaaatatt aaaatccaag
     3001 ttaaaagatt aaaagataat acagctctcc ctgcttatat gactgaaggc tcttcgggtt
     3061 tggatctgta ctctgctgag gagaagatta tccccccagg gaaatgggag gtgatatcta
     3121 caggtatagc ggtagaaatc ccttatggat atgagggtga gatcaggtcc agaagtggaa
     3181 tcgctaagga tcatggcgtt tttgtcctga atagtcctgg gactgtggac agtgattata
     3241 gaggagaagt aagagttata cttatgaatc ttggagataa atcttttaat ataaatacag
     3301 gggatagaat agcgcaactg gtaataagtc cagttgtgca ggtagaggta atggaggtcg
     3361 atgaactctc agacaccggg aggaatgctg gtggttttgg gcatactggt aggaggtaaa
     3421 atatggtaag agtaatggtt tgtggtgctt atggcaggat gggacaggaa gttataaagg
     3481 ctttatctga gcagaaggat atgatgatag taggggctat agatgtaaga ggggtagggg
     3541 agagtgtatt cggaataact gtgagagata atcttgagga ggtcataaaa gagaccagac
     3601 cctctgctat tgttgatttc actaaaagag aagctgcatt agaagatata aaaacaggtg
     3661 ttaacggcca ggatctggtg ggtttggttt ttcacac
//
LOCUS       DAOTKU010000124         3694 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_20966_length_3694_cov_2.569112, whole genome shotgun sequence.
ACCESSION   DAOTKU010000124 DAOTKU010000000
VERSION     DAOTKU010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3694
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20966_length_3694_cov_2.569112"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="P5310_03955"
     CDS             <1..455
                     /locus_tag="P5310_03955"
                     /inference="COORDINATES: protein motif:HMM:NF013813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HRU32548.1"
                     /translation="EDIPVLLDFSPVIDFPLVAGDRTFNATILSTGTTHTVIFFKDLP
                     DEIFLAYSPRIENHSIFPERTSVIWVTEEDEDELRVRIWERGVGETLSCGTGACAAVV
                     AKILKNGNRTNYRVTSKGGTLEVNWRDKKDILLSGKVESVFRGNLELR"
     gene            complement(473..1273)
                     /locus_tag="P5310_03960"
     CDS             complement(473..1273)
                     /locus_tag="P5310_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRU32549.1"
                     /translation="MVEDRELLRVENLTRIFGSGKSQVRAVDNVSFSIKEGEIVSLLG
                     ESGSGKTTTARLILKLLPPTSGKIIFREKDITYVSSRKELLNYWREVQAIFQDPFSSF
                     NIFYSIRRVLNNALRLVSESISREEREEMIKEVLLSVGLNPAEVLDKRPYELSGGQRQ
                     RIMLARVLLIRPKLLVADEPTSMIDASSRAGILNLLMELRNAYGMSIIFITHDIGLAY
                     YTSDRLFIMNSGKIVEEGDAEEIINNPKDTYTKKLLEDVPVIHRKWEL"
     gene            complement(1266..2255)
                     /locus_tag="P5310_03965"
     CDS             complement(1266..2255)
                     /locus_tag="P5310_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013304256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRU32550.1"
                     /translation="MGNLLTATGLKSYYRTVQGEEIKAVDDVSLEVREGEVLGIAGES
                     GCGKSTLASVLSFTVLPPLYILGGEVRADGNNILVMDRETLRREIKGKYISIVPQGAM
                     NSLNPTMRIKNFVIDVLEEHFPEISKEEALQRARERFEALSLPSRVLDAYPHQLSGGM
                     KQRTVTVISTLLNPNVLIADEPTSALDVSSQKIVIKLLVELLRRKIIKSVVFITHELP
                     LLRHFADRIAIMYAGKIVEIGPMEDIIFNPFHPYSRLLTSSVLVPEPGMKQKKIEAIP
                     GVPPNLKNPPSGCRFHPRCPSYMEGLCNIEIPPLIEVKEDHRVSCFLYGGKNG"
     gene            complement(2258..3097)
                     /locus_tag="P5310_03970"
     CDS             complement(2258..3097)
                     /locus_tag="P5310_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRU32551.1"
                     /translation="MKDIFILLFRSKRFIVGFVLFLAVLSFAIIGPMLNPKNPFQMVG
                     GLFTPPSDKFILGTDNLGRDVFTQLMYGTRTSLLIGCTAGVIATSIGVTIGSIAGYKG
                     GIVEEILMGITNVFITIPSIVVLILLSIAIKTRSAAVMGLVIGVTSWPWTARAVRAQT
                     ASLKTREHIDIARITGFSSIEMIVLEILPYMFSYIFMAFILQFSGGILNEATLSMLGL
                     GPYQTVSLGVMLHWSLLWEAVRTGAWWAFIPPVICLSMTTFSLMSMNSSMDEVFNPRL
                     RRS"
     gene            complement(3099..>3694)
                     /locus_tag="P5310_03975"
     CDS             complement(3099..>3694)
                     /locus_tag="P5310_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007130875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRU32552.1"
                     /translation="DSVAFTLSLVFSNIPYYCLAIVFLYLLAVKFAIFPVGGGYSFGT
                     VPTLSWRFIVDAIHHYILPYCSMVLVAIGGQAIGMREMSIYELTSDYVNYARNLGIRD
                     EKIIRYVFRNAMLPQITGLAISFGTMMGGALVTESVFSYPGVGSLLFTAIRQTDYPLI
                     QGCTLIITIAVLVANFLVEIAYGFIDPRIRAVQLGEK"
BASE COUNT         1120 a          829 c          714 g         1031 t
ORIGIN      
        1 cggaagatat acctgtattg ctggactttt ctcccgtgat agattttccc cttgtagctg
       61 gagacaggac attcaatgcc accatcctct caacgggaac cacacatact gtaatattct
      121 ttaaagactt accagatgag atattcctgg cttacagtcc caggatagag aatcattcaa
      181 tcttcccgga aaggacaagt gtaatctggg ttacagaaga agatgaagat gagttaaggg
      241 tacgtatatg ggaaagaggc gtgggagaaa cattgagctg tgggaccgga gcctgtgccg
      301 cagttgtggc aaagatattg aaaaacggaa atagaacaaa ctacagggta acatcaaaag
      361 gtgggacgct tgaggtaaac tggcgggata agaaggatat acttttatcg ggaaaggtag
      421 aaagtgtatt caggggcaac ctggaattga gataaaaata aagaggggag ccctatagct
      481 cccactttct atggattaca ggaacatctt cgaggagttt tttcgtataa gtatctttgg
      541 gattgtttat tatctcttct gcgtctccct cctcgacaat tttaccgcta ttcataataa
      601 ataatctatc gcttgtataa taggctaatc ctatatcgtg cgtgatgaag attatagaca
      661 taccataggc atttctgagt tccataagca aatttaaaat tccagccctg gaagaggcat
      721 ctatcatcga cgtgggttcg tctgccacaa ggagcttcgg tcttataaga agaactctgg
      781 caagcattat tctctgtctc tgcccaccac tcagttcata tggacgttta tcaagaactt
      841 ccgctggatt taagccaaca ctcaacagga cctcttttat catctcttct ctctcttctc
      901 tggaaataga ttcagaaacg agccttaagg cattgttcaa tacccttctt atagaataga
      961 agatattgaa agatgaaaat gggtcctgga atatagcctg tacctctctc cagtagttca
     1021 ataattcttt ccttgaggaa acgtaagtga tatctttttc tctgaatata atctttccac
     1081 tggttggtgg cagcaatttt aatatcagcc tggcggtggt cgtcttacca ctgccactct
     1141 ctcctagaag tgaaactatc tccccctctt taatagaaaa agatacatta tctactgctc
     1201 ttacctgaga tttcccggat ccaaaaatcc tcgttaaatt ttcaaccctt agaagttctc
     1261 tatcctcaac cattctttcc tccgtacaga aaacaactga ccctgtgatc ttcttttacc
     1321 tctattaagg ggggaatctc aatattacat agtccctcca tatatgaagg gcatctggga
     1381 tggaatctgc atccagatgg aggattcttt aaattaggtg ggacaccagg gatagcctct
     1441 atcttcttct gcttcattcc aggttccggt accagaactg aagatgtaag aagcctgctg
     1501 tatggatgaa aaggattaaa gataatatct tccataggac caatctccac tatctttccg
     1561 gcatacataa ttgctattct atcagcgaaa tgtctcaaga gtgggagttc atgggttata
     1621 aagaccacac tctttataat ctttcttctt aaaagttcca ccagtaactt tattacaatc
     1681 ttctgactcg agacatcaag agcagaggtg ggttcatctg ctatcaacac attcggattt
     1741 aataatgttg aaattacggt gactgttctc tgtttcatac ccccactaag ctgatgggga
     1801 taggcatcca gcacccttga gggtaatgaa agggcttcaa atctctctct ggctctctgc
     1861 aatgcctctt ctttagatat ttcaggaaaa tgttcttcca gaacatctat aacaaaattt
     1921 ttaattctca ttgtaggatt cagagaattc atagctccct gaggaacaat tgaaatatat
     1981 ttacctttga tttctctcct gagggtctct ctatccataa ccaggatatt atttccatct
     2041 gcccttacct ctccacccag tatgtacagt ggaggaagaa cagtaaaaga taagaccgat
     2101 gccagggtag actttccaca ccccgattct ccagctattc caagaacctc accttctctt
     2161 acctcaagag agacatcatc taccgccttt atctcttccc cctgtacagt tcggtagtag
     2221 gacttcaaac cagttgcggt caagagattc cccataatca gctccttctc aacctgggat
     2281 tgaagacttc atccatgctg gagttcatac tcataagtga aaaggtcgtc attgacaggc
     2341 atattactgg ggggataaaa gcccaccatg cacctgtcct tacagcttcc cagagaagcg
     2401 accagtgtaa catcacaccc agagatactg tctggtaagg accaagacca agcatactca
     2461 atgtagcctc attcaatata ccacctgaga actgaagtat aaaagccata aatatgtaag
     2521 aaaacatata gggaagtatt tccagaacga tcatctctat agagctaaaa ccggttatcc
     2581 ttgctatatc tatatgctct ctggttttca ggcttgctgt ctgagctctt accgctctgg
     2641 ctgtccaggg ccaggaggtt acccctataa ccagacccat cacagctgct gacctggttt
     2701 taatagctat agaaagaagg atcagtacaa ctatagaggg tatcgtaata aacacgttgg
     2761 ttatccccat caatatctct tccactatcc cccccttata accagcaatg gaaccaattg
     2821 taacacctat actggtagca ataacaccgg ctgtgcatcc aatgagaaga gaagttctgg
     2881 ttccgtacat aagctgggta aaaacatctc tccccagatt atctgtaccg aggataaact
     2941 tatcagatgg aggagtgaag agtccgccta ccatctgaaa tggatttttg ggatttaaca
     3001 tcggacctat aatagcaaaa gaaagcactg caaggaatag aacaaaaccg actataaatc
     3061 gtttggatct gaacagtaat ataaatatgt ctttcatatt atttctcccc cagttgaacc
     3121 gctcttatac gcgggtctat aaatccgtag gcaatctcaa ctaaaaagtt tgccacaaga
     3181 accgcaatgg ttattataag agtacagccc tgtatgagcg gataatcggt ctgtcttata
     3241 gcggtaaata ataagcttcc cacccctgga taggagaaaa cagattctgt taccagcgcc
     3301 cctcccatca ttgtaccaaa ggagatagca agccctgtaa tctgcgggag cattgcattc
     3361 ctgaaaacat acctgataat tttctcgtct cttatgccaa gatttctagc ataattaaca
     3421 tagtctgaag taagttcata tattgacatc tctctcatcc ctatagcctg accacctata
     3481 gccacaagga ccatagaaca gtaaggaagt atatagtgat gaatggcatc cacgataaat
     3541 ctccacgata gagtggggac tgtaccaaaa ctgtaacctc caccaacagg aaagatggca
     3601 aatttaactg ccagcagata caggaaaact atagcaagac agtagtatgg gatatttgag
     3661 aatacaagag atagggtaaa ggctacgcta tcaa
//
LOCUS       DAOTKU010000125         3689 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_21006_length_3689_cov_3.049257, whole genome shotgun sequence.
ACCESSION   DAOTKU010000125 DAOTKU010000000
VERSION     DAOTKU010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3689
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21006_length_3689_cov_3.049257"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="P5310_03980"
     CDS             <1..185
                     /locus_tag="P5310_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011916695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HRU32553.1"
                     /translation="MHFELIDFSLKSVTTGKDALGESLVKLKKNGEIFTGRGTSKNVL
                     EASAIAYINAINRALV"
     gene            222..1478
                     /gene="leuC"
                     /locus_tag="P5310_03985"
     CDS             222..1478
                     /gene="leuC"
                     /locus_tag="P5310_03985"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HRU32554.1"
                     /translation="MGMTLTEKIISTHTDREEVHSGEFVVAKVDFSLANDITAPLAIK
                     EFQKIGVDKVFDEERIALIPDHFTPNKDIASAEQCKILREFARKMNIKYYYEVGRTGI
                     EHALLPEEGLVVPGDVFIGADSHTCTYGALGAFSTGVGSTDIANVWATGDIWLRVPES
                     IKFVLYGRPGRWVTGKDIILNIIGRIGVEGANYMSMEFSGEALKYISMDSRFTMCNMA
                     VEAGAKNGIIPFDDITAEYEKGRNKRPYRIYLPDEDAYYRDVMEIDVSAIPLEVALPH
                     LPSNVKAVEDAGDIYIDQVVIGSCTNGRMEDLRIAAQILKGRKVHPYVRLIVIPATQE
                     IYRQALKEGLIEIFISAGASVSTPTCGPCLGGHMGILAKGEKALATTNRNFIGRMGHP
                     ESEVYLCGPAVAAASSIEGKIVAPYD"
     gene            1489..1704
                     /locus_tag="P5310_03990"
     CDS             1489..1704
                     /locus_tag="P5310_03990"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HRU32555.1"
                     /translation="MLKTGERGKIVRIEGGMMVIKRLTDLGFVPEQEVEVIRSAPRGP
                     VAIKIKGAVYALGRGIADKIYVEEVHE"
     gene            1682..3646
                     /gene="feoB"
                     /locus_tag="P5310_03995"
     CDS             1682..3646
                     /gene="feoB"
                     /locus_tag="P5310_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012269720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HRU32556.1"
                     /translation="MLKKSTSRSLDSHISKKEITIALAGNPNAGKSVVFNQLTGGNQI
                     IGNWPGKTVERTEGWIRYRDRRIRIVDLPGIYSLSTYTEEELIAREFIISGIPDVIIN
                     VIDASLLERSLFLTLQLLEMEKPLVLAVNQLDVAESKGLKTDIDALSKLLSIPAVGVI
                     ATRGTGLNKLLTVAIESADKQDSVAPPLYGGEIEQSIEELEKILDNAYPEYPVRWLAI
                     KIIENDEDIKRLHLPEEVLERADVIRRRLEETHGEPIEVILSSERYALASRIARTSQR
                     YEVGRKTLLQRLDSVLLHPVFGYISMGFFLFVAFIVLFKIGSIGSSLLEGLFSRLAPM
                     VNSLALPETVKELLWDGLLSGIISGITIALPYILPFYIFLSFLENSGYLARIAFLTDN
                     IMHKLGLHGKGAIPIIMGYGCNVPAILGTRILESRRERVIASLLATLIPCSARTVVIL
                     GLVGAFMGLKWAFVIYLLDLVIVYMAGFLMNRYYPGETPGLIMEVPDIRIPPLKPSLR
                     QSWFRIKEFLYIALPYIMLGSVILELIKIFGWFDTINRILSPVIVNWLGLPPITGVVL
                     IFGLLRKELTLILLSTFSGTTNFSTILNPRQMFVFSFFVTLYVPCLATVAALKKELGN
                     ITSTLIVIGEILISLILAGLLNHIWIYMAG"
BASE COUNT         1060 a          636 c          901 g         1092 t
ORIGIN      
        1 aaatgcactt tgaacttata gatttctctc tcaagtctgt taccacagga aaggatgcac
       61 tgggagagtc tctggtgaaa cttaagaaga atggagagat attcaccggt agaggtacca
      121 gcaagaatgt acttgaagcc agtgctattg cttatataaa tgctataaat agagcacttg
      181 tctaattctt atttgattga tataattatg agggaaagac tatgggaatg acacttactg
      241 aaaagattat ttcaacacat actgataggg aagaggttca cagcggggag tttgtagtcg
      301 ctaaggtaga tttttctctt gcgaacgata ttacagcccc tttagctata aaggagtttc
      361 aaaagatagg tgtagataaa gtttttgatg aggagaggat tgctcttatc cctgaccatt
      421 ttacacccaa caaagatata gcaagcgctg aacagtgtaa aatattgaga gaatttgcac
      481 gtaagatgaa tatcaaatac tactatgagg tagggcggac aggtattgag cacgcccttc
      541 ttcctgaaga gggacttgtt gtccctggag atgtatttat tggcgcagat tctcacacct
      601 gtacttatgg tgctttggga gccttttcta caggggttgg aagtacagat atcgctaatg
      661 tgtgggcaac tggtgacatc tggctcaggg ttcctgagag tataaaattt gtcctttatg
      721 gtagaccggg aagatgggtt accggtaagg atatcatatt aaacattata ggtcgtattg
      781 gtgtggaagg ggcaaattat atgtctatgg aattttctgg agaagcactg aagtatatat
      841 caatggattc aaggtttacc atgtgcaata tggctgtaga ggctggtgcc aagaatggca
      901 ttataccatt tgatgatatt acggcagaat acgaaaaggg tagaaataag agaccgtaca
      961 ggatatatct tccagatgag gacgcctact atagagatgt aatggagata gatgtttcgg
     1021 ctattccttt agaagtagca cttccccatt taccatcgaa tgtgaaagcg gtggaggatg
     1081 ctggagatat ttatatagac caggttgtta taggctcctg taccaatggc aggatggaag
     1141 atctcaggat agccgcccag atcttgaagg gaagaaaggt acatccatat gtaagactta
     1201 tagttattcc ggctacccag gagatttatc gtcaggcttt aaaggaagga cttatagaga
     1261 tctttatatc tgctggtgcc agcgtttcta cacctacctg tggtccctgt ttggggggac
     1321 atatggggat cctggctaaa ggagaaaagg ctttagccac tacgaataga aactttatag
     1381 gaaggatggg gcatccagaa agtgaagtct atctctgtgg tccggctgtt gcagctgctt
     1441 cgagtataga gggaaagatt gtagcccctt atgattaaac ctctatccat gttaaagaca
     1501 ggagagagag gaaagatagt caggattgaa ggcggtatga tggtaataaa gagattgact
     1561 gacctgggat ttgtccctga acaggaggtt gaagttataa ggtctgcacc cagaggtcct
     1621 gttgccataa agataaaagg ggcagtgtat gcccttggca gagggatagc agacaaaatc
     1681 tatgttgaag aagtccacga gtagaagttt agattcacat atatcgaaaa aagagataac
     1741 aatcgctctt gccggtaacc ctaatgctgg caagagcgta gtttttaatc agcttactgg
     1801 tggtaatcag attataggca actggccagg taagacagtg gagaggactg agggatggat
     1861 tagatacagg gatagaagaa taaggatagt agaccttccc ggaatttata gtctctctac
     1921 atataccgaa gaagaattga tagccagaga gtttataatc tccggaatcc cggatgtgat
     1981 aattaatgtt atagatgcct cccttctgga gaggagtctc ttcctgaccc tgcaacttct
     2041 ggagatggaa aaacccttgg ttcttgctgt aaatcaactt gatgtggcag aatcaaaggg
     2101 gttaaaaact gatatagatg ccctttccaa acttcttagc atcccggctg ttggagttat
     2161 agccaccagg gggacgggat taaataaact tttgaccgtc gccattgagt cagcagataa
     2221 acaggatagt gttgccccac ctttgtacgg tggagagata gagcagagta tagaagagct
     2281 ggaaaagata ctggataacg cctatccaga atatcctgtc aggtggctgg ctataaagat
     2341 tatcgaaaat gatgaggata taaagagact ccatctacca gaagaagttt tagaaagagc
     2401 agatgtaata aggagaaggc ttgaagaaac tcatggggag ccgatagaag ttatattatc
     2461 ttcagagaga tacgctctgg ctagccgtat agcgaggacc tctcagcgct atgaagtggg
     2521 taggaaaacc cttcttcaga ggctggattc agtcctgctt catcctgtct ttggatatat
     2581 ctctatgggg ttcttcctct ttgtagcgtt tatcgtcctt tttaaaattg gttctattgg
     2641 ttcttctctt ctggagggtc tcttctccag attagcccca atggttaata gtcttgcact
     2701 tcctgaaact gttaaagagc tcctctggga tggtctttta agtgggatta tatctggtat
     2761 aacgatagcc ctcccctata tcctgccatt ctacatattt ttatcttttc ttgagaatag
     2821 tggttacctt gctcgtattg cgtttttaac agataatatt atgcacaaac ttgggcttca
     2881 cggtaaggga gctataccca ttattatggg ctatggttgc aatgtcccgg ctattttggg
     2941 tacgagaatt ctggagagta gaagagagag ggtcatagcc agtcttcttg ctacacttat
     3001 accctgttct gccaggacgg ttgttatcct ggggcttgta ggtgccttta tggggttgaa
     3061 gtgggctttt gttatatatc tcttagattt agttatagtt tatatggctg ggtttctgat
     3121 gaatagatac tatcccggtg agactccagg gctcattatg gaagtccctg atataagaat
     3181 accgcctctc aagccaagtc taagacagtc ctggtttaga ataaaggaat tcctatatat
     3241 agctctcccc tatataatgt tggggtctgt gattttagaa ttgataaaga tttttggatg
     3301 gtttgatact ataaatagga tactgagtcc tgttatagtg aattggcttg gacttcctcc
     3361 aataactgga gttgttttga tatttgggct gttaaggaag gaacttaccc tgattttact
     3421 ctccacattc agtggaacta caaatttctc aacgatatta aaccccaggc agatgttcgt
     3481 tttttccttc tttgtaacac tttatgtacc ctgtctggct acggtggcag ccctgaaaaa
     3541 agagttgggt aatattactt ccaccttaat tgttattgga gagatactta tctctttgat
     3601 cctggctgga cttttaaatc atatctggat ctatatggct ggatagataa ggaggtgaat
     3661 tgtttgtcaa tagtaacaat atctagaga
//
LOCUS       DAOTKU010000126         3645 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_21306_length_3645_cov_4.108635, whole genome shotgun sequence.
ACCESSION   DAOTKU010000126 DAOTKU010000000
VERSION     DAOTKU010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3645
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21306_length_3645_cov_4.108635"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            315..683
                     /locus_tag="P5310_04000"
     CDS             315..683
                     /locus_tag="P5310_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32557.1"
                     /translation="MRRILSILIITGILTGYFCLTSFACEMRLTPDRFTAKVGEKITF
                     TLERIQSHKNCVLPLEATKIEVKGGKVVDPGKWIKKDNADILRFTVQFTTPGEAVVTV
                     IRDCPKEGIMRVEARGTVLK"
     gene            complement(700..1932)
                     /locus_tag="P5310_04005"
     CDS             complement(700..1932)
                     /locus_tag="P5310_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRU32558.1"
                     /translation="MKISIRLTLIYSLILIFFIIFSAVFFYWNTERLLLDEIRKELLR
                     GPSIEDFLTRGTGYGKLSSLYIVSKSVVVQDPFGLGFKDKEGIHKVNGIYVFLVERED
                     FLVGKDITPTILALNRLKSNIILLSLFSVVVSLIVGYILSQRFLSPLRDMIRTARDIN
                     VRDLDKRIGLPGVKDELFDLALTINNMLERVSSAYKMQEQFILDVSHELRTPLTSLLG
                     HIRLLQRWGKEDKKILEESLESIRETAEDMERLINTLLETLKTQEDVSLGPIDLESFL
                     EKRKEYYEKLYPDFQFDIILDDPKREIYSSLPILELVFNILVENAVKNSPDKKVVELG
                     WERDSFFVRDYGRGIPQEERERIFERFYRLDDSRSDGGYGLGLSLAKKLVQILNFKIY
                     VESEEGKGSTFYIVTVER"
     gene            complement(1929..2600)
                     /locus_tag="P5310_04010"
     CDS             complement(1929..2600)
                     /locus_tag="P5310_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRU32559.1"
                     /translation="MKKVLVVEDDPKISRVLELELSHEGYITKVVDDGYDALVESEDF
                     KPDVVILDILIPGINGKEVAKRLRNRNPLLGIIMLTALGDIRDKLEAFRYGADDYLVK
                     PFNIEELVARIEAISRRKTSIKETSLSLYGIELFPDQHRLLIDGKDVELSKTEFSLLY
                     LLMANEGIVLSKDRILEVVWEDYDGGRENLVEVYINYLRKKLGEKGKYIKTIRGVGYT
                     FRGDA"
     gene            2723..3478
                     /locus_tag="P5310_04015"
     CDS             2723..3478
                     /locus_tag="P5310_04015"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRU32560.1"
                     /translation="MIQLIELLSRYQLWIIFLIAGIILYLLKLHPIRKVMLFLSVIVL
                     GFYLGGCPCPSGSVFHLFTGVGSFLFIISTITTLLFGRVYCGWVCPLGAVQELIFTGR
                     YRIRIPENFDRYSKYVKFLILATFLYLTISTSTYTWGKYEPFKVLFNFGGNRIAFILL
                     LITMGLSMFIERVFCRYICPMGAVFSIISRFSIFGLRINKDRCVNCLRCTRDICPMGA
                     IETTHDKIKIDKRECIDCLKCKDICSKGAPTCK"
BASE COUNT         1137 a          762 c          593 g         1153 t
ORIGIN      
        1 gtcaatacct taatgaatga gtttttaatc tttaccctga aatcagtcct ggaactcttt
       61 ttactgcagg attctcactc agaacatacc agagtcctcc agcatggtcc tggtgttcgt
      121 gagaaataac aatgtatttt aatcttttta tatcaatatt taagctggag aaatttttct
      181 tgaggataat ataggtggag ataaactaaa ggtctaagca aatcttaagt tttttcttat
      241 tttcttctaa gaccatcatt ttaaagttga tatagaagag tcaaaaataa aaattaagca
      301 tggaggcact aaaaatgaga aggatattgt ccatattaat tattacaggt atattaacag
      361 gctacttttg cctgaccagt tttgcctgcg agatgaggct aacaccagat agatttacag
      421 ctaaagttgg agaaaaaatt acatttacac tcgaaaggat tcaatctcat aaaaattgtg
      481 tcctcccatt agaggcaaca aagatagaag ttaagggtgg taaggtggtg gacccgggca
      541 aatggataaa aaaggataat gcagacatat taaggtttac cgttcaattc accaccccgg
      601 gggaggcggt tgtaactgta attagagatt gccctaaaga aggtataatg agggtagaag
      661 ccagagggac agtgttaaag tgacatcaaa gagcaccttc tatctctcca cagtaacaat
      721 atagaaggtg cttcctttcc cctcttcact ctccacataa atcttaaagt ttaaaatttg
      781 aacaagtttt ttagcaagag aaagacccag tccataaccg ccatcgctcc tggaatcatc
      841 aagcctataa aatctctcga aaatcctctc cctttcttcc tgagggatac ctctaccata
      901 atccctgaca aaaaaactat ctctctccca tccaagctct acaactttct tatcaggact
      961 attctttact gcattttcaa ccagtatatt aaatacaagt tctaatatgg gtaaggaaga
     1021 ataaatttct ctcttaggat catccagaat gatatcaaac tggaaatcag ggtaaagctt
     1081 ttcgtagtat tcttttctct tctccagaaa agattccagg tctatcggtc ccagagatac
     1141 atcctcttga gtttttaacg tctctaaaag cgtatttatc agtctctcca tatcctcagc
     1201 cgtctctctg atagattcaa gggattcctc cagtatcttc ttatcctctt ttccccacct
     1261 ctgtaaaagt ctaatatgtc ccaaaagaga agtcagtgga gtcctgagct cgtgagacac
     1321 atctaatata aactgttcct gcatcttata agcactgctt actctctcaa gcatattatt
     1381 tatagttaaa gccagatcga aaagctcatc tttaacacca gggagcccta tccttttatc
     1441 aaggtctctt acatttatat ctctggctgt ccttatcata tccctaagtg gagacaggaa
     1501 tctctgagag agtatatacc cgacaattag agaaactact acactgaaaa gagaaagcaa
     1561 tataatatta ctttttaacc tattaagagc caggattgtt ggtgttatat cttttccaac
     1621 taaaaagtct tctctttcga caagaaagac atatatacca ttaaccttgt gtatcccttc
     1681 tttatcctta aatcccaacc caaatgggtc ctgcacaact acacttttag agacgatata
     1741 aagagaagac aacttaccgt atcctgtccc cctggtaaga aagtcctcta tagaaggtcc
     1801 tctcaagagt tctttccgaa tttcatccag aagtaatctc tctgtattcc agtagaagaa
     1861 aacagcagag aaaataataa aaaatatcag tatgagtgaa tatataaggg taagtcttat
     1921 agatatcttc aagcatcacc tctaaaagta tatccgaccc ctcttatagt ctttatatat
     1981 ttaccctttt ctcccaattt ctttctgaga tagttaatat aaacctctac caggttctcc
     2041 cttcctccat cataatcttc ccagaccacc tccagtattc tatctttact caggacaatt
     2101 ccttcatttg ccatcaatag atataaaaga gaaaattctg ttttagaaag ttctacatcc
     2161 ttcccatcaa ttaacaatct atgctgatca gggaaaagtt caatgccata aaggctcaag
     2221 ctggtctcct ttatagatgt tttcctgcga gatatcgcct ctattcttgc cacaagctct
     2281 tctatattaa atggcttcac cagataatca tccgccccgt atctaaatgc ttcaagttta
     2341 tctcttatat ctcctaaagc ggtaagcata atgataccca aaagaggatt tctatttctc
     2401 agtctcttag caacttcttt cccatttatt ccaggaatca ggatatccag gataactaca
     2461 tctggcttga agtcttcgct ttctacaagg gcatcatatc catcatcaac cactttagtt
     2521 atgtatccct catgggagag ttcaagctcc agaacccttg atatcttagg gtcatcttca
     2581 acaacaagaa ctttcttcat actcttcctc caagattatt ataacatcaa atcaacaatt
     2641 ctaaggattc tttaagatta tccttagtaa atactaagtt ctcaatttta tactctaaca
     2701 aaactaaaac tggaggtcaa ttatgatcca actcatagaa ttattgagca gatatcagtt
     2761 atggataata ttcttaattg caggtataat tttatattta ttaaaattac atcccattag
     2821 aaaggtaatg ctctttctaa gcgttattgt ccttggattt tatcttggag gctgtccctg
     2881 tccaagcgga tctgtatttc acctttttac aggggtggga agttttcttt ttataatctc
     2941 cacaataacc actttactgt ttggaagggt ctactgtgga tgggtatgtc ctctaggcgc
     3001 agtccaggaa ttaatcttta caggcaggta caggatcaga atacctgaga attttgatag
     3061 atactcgaaa tatgtaaaat ttcttatcct ggcgactttt ctctatctta caatctccac
     3121 gtcgacatac acctggggta aatatgagcc atttaaggtc ctgttcaact ttggaggtaa
     3181 caggatagcc ttcatacttc tattaataac catgggacta tctatgttta tagaaagagt
     3241 cttctgcaga tatatatgcc cgatgggagc agtattctcc attatttcca gattctccat
     3301 ttttgggctg aggataaaca aagatagatg tgtaaattgt ctgcgttgca ccagggatat
     3361 atgcccaatg ggtgcaattg aaaccactca tgataaaatt aaaattgata agagagagtg
     3421 tatagattgc ctgaagtgta aggatatatg ctcgaaaggg gccccaacgt gcaaatagag
     3481 cccctctatc tttctcagga taatctgatc gcctgattta ataactggat actggccagt
     3541 atcccctctt cagaaggtgc tttcccctca aattcgatag agagataacc tttataatca
     3601 gcatctttaa gctttctaac tattcttata tagtcaggat taagc
//
LOCUS       DAOTKU010000127         3643 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_21321_length_3643_cov_3.018952, whole genome shotgun sequence.
ACCESSION   DAOTKU010000127 DAOTKU010000000
VERSION     DAOTKU010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3643
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21321_length_3643_cov_3.018952"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="P5310_04020"
     CDS             <1..186
                     /locus_tag="P5310_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32561.1"
                     /translation="YEYFKFGDWKDDLSKLGVSKGLIEIKRIEESSSTIDNLLSVRIS
                     VEWGKDKSYEITTYISE"
     gene            332..646
                     /locus_tag="P5310_04025"
     CDS             332..646
                     /locus_tag="P5310_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32562.1"
                     /translation="MSLERIIRESRGAKGNITIESANPDREGFKFQKANGTEFIGFYR
                     YKNDDKIRRYVDNKAGNIIATNVASFSVTKKDNGSYEIKIEFNQDRDSYTVSGVFTPR
                     NK"
     gene            660..1730
                     /gene="pilM"
                     /locus_tag="P5310_04030"
     CDS             660..1730
                     /gene="pilM"
                     /locus_tag="P5310_04030"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HRU32563.1"
                     /translation="MRLRGRKSMVGIDLGTYSLKAVEVESREDGFYLNRATSIFYPEK
                     VLEGGEFQNVPLFTQLIDKLWKNGDFGTRNVSISWKMENGFFRVLTLPLLPDKELFEA
                     VKLEIGSRYDVDASLISFDYIILDKDEKNQEIKVLTAGIPRVTANTVYDVLKEVNLTL
                     KVLEPEFICQLNVLPPVEESFMLLNVGAYATILYMGMMDTINVIRTLRFGGNTATDVI
                     SSTLNISFEEAEIWKKDRFSEEVGYAKTSVGNALLDTFAVFLREVNRSIDYFYQVTNS
                     YPTRIILDGGGAFLLGLADYISRGLSLPVEMLDMGKVVKKNVDLENLNFYSVAIGAAL
                     SGLDYKCPYKFFERLELGRIVV"
     gene            1730..2263
                     /locus_tag="P5310_04035"
     CDS             1730..2263
                     /locus_tag="P5310_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32564.1"
                     /translation="MLDLTREFKREESKLKLQSLLQTILILVFIVNALVTYYILWLRG
                     NIMRAEIEGIDNQLTLLLPIEERINKKTAELNSLIAKRREIENKIETPKMNLDLIRNV
                     SIYAPSDFWLSNLSISSTDIIFDGYGLSIRGIKNFIQSIVDNTQGSSLSRTSIQKVKL
                     GGTECYNFHIEISRGKL"
     gene            2260..2784
                     /locus_tag="P5310_04040"
     CDS             2260..2784
                     /locus_tag="P5310_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32565.1"
                     /translation="MKINIRDWRIIILISVVFYVLWMSFLVYPQGLKVQEMQQELNEK
                     RLKLSRAQFYPVRERQLDSAISQEKTLIEELSVRLYSYTVIANLLKDIEDISRKYGIE
                     ILSISGPNKLEDRVSFNFTLKGNYTEFVPWAYELSMSKTLNITQLNAKIEGDNIIYSG
                     LLETTPFQEVKVGQ"
     gene            2774..3403
                     /locus_tag="P5310_04045"
     CDS             2774..3403
                     /locus_tag="P5310_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32566.1"
                     /translation="MGSRIIFIVIISLVCISLAGTVIFINRFTKEEFQEIPSIPPPVP
                     ELPEKTVQSVFYYPPEIYRDPFKVPIRIKPLEEMVKTEVPQQETSQKVSAPEEESSPA
                     VTPVQKVQPVVKSKPETVQTEGKVPVVTRTEGEKIQQKISLPKVIVTGIIYDDEPFAI
                     LEFEGRSGIFEKGDKLSGDLSVKKIYADSVDIDWKGRVYNIKLGGKYEN"
     gene            3393..>3643
                     /locus_tag="P5310_04050"
     CDS             3393..>3643
                     /locus_tag="P5310_04050"
                     /inference="COORDINATES: protein motif:HMM:NF023169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HRU32567.1"
                     /translation="MKTNIVLILCLLLVLSSVNISLGKSLADVSWHIEGDKLIINLKA
                     SDKIAYTTFTLSDPIRFVIDVYNVTIPDPKSIEINKPPV"
BASE COUNT         1221 a          501 c          788 g         1133 t
ORIGIN      
        1 tatgagtatt ttaaatttgg tgactggaaa gatgaccttt caaaactagg ggtaagtaag
       61 ggtctaatag agataaagag aatcgaagag agtagctcaa ccattgataa tctccttagt
      121 gttagaatta gtgtagagtg gggtaaagat aagagttatg aaatcaccac gtatatatca
      181 gaataataga aaaggtttta cccttattga attgttgatt gttttactcc ttttttcttt
      241 tgtaattgct ttgatatata atatatataa tatggggatt tttgtttgga aaagcactga
      301 aagaagatta tggactgtac aggaggccag gatgtcgctg gagaggataa tcagggagtc
      361 cagaggggca aagggaaata tcactattga aagtgccaat cctgatagag agggatttaa
      421 atttcagaaa gcaaatggta ctgaatttat aggattttat agatataaga atgatgataa
      481 gattcgaaga tatgtggata ataaagctgg taatattata gctacaaatg tagcttcttt
      541 ctcggtaact aagaaagata atggttcata tgaaattaag atagaattta atcaggatag
      601 agattcttat acagtttcag gggtatttac ccccagaaat aaataatagg gagtggttta
      661 tgagactaag aggacgtaag agtatggtgg gaattgatct aggaacgtat tctttaaagg
      721 cggtagaggt agagtctagg gaagacggat tttatctgaa cagggcgacc tctatctttt
      781 atcctgaaaa ggtcctggaa ggtggagagt tccagaatgt ccctcttttt acccagttaa
      841 ttgataaatt atggaaaaat ggagattttg gtaccagaaa tgtttctatc tcctggaaga
      901 tggagaatgg tttcttcagg gttctaaccc tgccattatt gccagataaa gaattatttg
      961 aggctgttaa attagagata ggtagcagat acgatgtgga tgcgagttta atatcgtttg
     1021 actatattat cctggataaa gatgaaaaaa atcaggagat aaaagtttta acggcgggaa
     1081 ttcctagagt tactgctaat actgtttatg atgttcttaa agaggttaat cttactctca
     1141 aggtccttga gcctgaattt atttgtcaac ttaatgtcct tccccctgta gaagagagtt
     1201 ttatgctact taacgtgggt gcttatgcta caatcttata tatggggatg atggatacga
     1261 taaatgttat aagaaccctc agatttgggg gaaatactgc tactgacgtt atttcctcaa
     1321 ctttaaatat atcttttgaa gaggctgaga tatggaaaaa ggatcgattc tcagaagagg
     1381 ttggatatgc caaaacatca gtgggtaatg cactcctgga tacttttgca gtgtttttaa
     1441 gggaagtaaa caggtcgata gattatttct accaggttac aaactcttat cccacccgga
     1501 ttattttaga cggaggggga gcttttcttt taggattagc tgattatata tctagaggac
     1561 tttcacttcc agttgagatg cttgatatgg gtaaggttgt aaaaaagaat gtagatttag
     1621 aaaacttaaa tttctattct gtggctatcg gagctgcatt gagtggactt gattataaat
     1681 gcccatataa attttttgaa agacttgagc tggggaggat agttgtctga tgttagacct
     1741 gactagagaa tttaaaagag aagagagcaa gttaaagtta caatccctcc tgcagacaat
     1801 tcttatcctt gtttttatag taaatgcact tgttacatac tatatactct ggctcagagg
     1861 gaatattatg agggcagaaa tagagggtat agataatcag cttacattgc tcttacctat
     1921 cgaagagaga ataaataaaa agacagcgga attaaatagc cttatagcaa agaggaggga
     1981 gatagagaac aagatagaaa ctcccaaaat gaatcttgat cttataagaa acgtatccat
     2041 atacgcacct tctgatttct ggctttctaa tttatccatt tcgtccacag atataatttt
     2101 tgacggatat ggcttatcta taagagggat aaagaatttc atccagtcaa tagtagataa
     2161 cactcagggg agtagtcttt caaggaccag tatacagaaa gtaaaactgg gtggaactga
     2221 atgttataat tttcatatag aaatttctag gggtaagtta tgaagataaa tattagggat
     2281 tggaggatta taatacttat atctgtggtg ttttatgtgt tgtggatgtc ttttctagta
     2341 tatccacagg gtctaaaggt tcaggaaatg caacaggaat taaatgagaa gaggctaaaa
     2401 ttatccaggg ctcaatttta tcctgtcagg gaaagacaac ttgatagcgc aatatctcag
     2461 gagaagactc ttatagaaga actgagtgtt agactttact cttatactgt aatagcaaat
     2521 ctactgaagg atatagaaga tattagtagg aaatatggca tagagattct tagtattagt
     2581 ggtcctaaca aattagaaga cagagtaagt tttaatttta cattaaaggg taattataca
     2641 gaatttgtcc cctgggctta tgaattgagt atgtccaaaa cacttaacat tacgcagcta
     2701 aatgcaaaga ttgagggtga taatatcata tacagtggtc tcctggagac gactcctttt
     2761 caggaggtta aagttgggca gtagaattat tttcattgta attatttcct tagtatgtat
     2821 cagcttagca ggaacagtta tttttataaa tagatttact aaagaagaat ttcaagagat
     2881 tccatctatt cctcctccag tccctgaatt gccagaaaaa acagtccagt cagttttcta
     2941 ttatcctcct gaaatataca gagatccatt taaggttcca ataagaataa aacctcttga
     3001 agagatggta aagacagaag tccctcaaca ggaaacttct cagaaggtat cagctcctga
     3061 ggaggaatct agtcctgcag ttactcctgt gcaaaaagtt cagcctgtgg taaagtcaaa
     3121 gcctgaaact gttcagactg agggtaaagt accggtggta accaggactg aaggagaaaa
     3181 gatccagcag aagatatctt tacccaaggt tatagtaaca ggtattatct acgacgatga
     3241 accatttgct atactggaat tcgaaggaag gagcgggata tttgaaaagg gggataaatt
     3301 atctggagat ttatcggtta agaagatata tgctgattca gtagatattg actggaaagg
     3361 tagagtctat aatataaaac tagggggtaa atatgaaaac taatattgtt cttattttat
     3421 gtttattatt agttctttct tcagttaata ttagtcttgg aaagagttta gctgatgtta
     3481 gctggcacat agaaggcgat aaattaataa taaatttaaa ggcttcagat aaaattgcct
     3541 atactacttt tactctgtct gatcctatcc gttttgtaat tgatgtttat aatgttacta
     3601 ttcctgaccc taaaagtatc gagataaata agcctccagt aaa
//
LOCUS       DAOTKU010000128         3621 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_21454_length_3621_cov_3.685081, whole genome shotgun sequence.
ACCESSION   DAOTKU010000128 DAOTKU010000000
VERSION     DAOTKU010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3621
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21454_length_3621_cov_3.685081"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(87..1076)
                     /gene="floA"
                     /locus_tag="P5310_04055"
     CDS             complement(87..1076)
                     /gene="floA"
                     /locus_tag="P5310_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005841555.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HRU32568.1"
                     /translation="MENTVIAYWLPIVVIVIALILFFQFVPLGLWISAIAASVKVTIV
                     TLIGMRLRRVPPHKIVLPLIKATKAGLDLNINQLEAHYLAGGNVDRVVDAIIAAERAG
                     IPLNFTRAAAIDLAGRDVLEAVQMSVNPKVIETPIVAAVAKDGIELKVKARVTVRANI
                     ERLVGGAGEATIIARVGEGIVTTIGSSETHKQVLENPDKISRTVLEKGLDAGTAFEIL
                     SIDIADVDVGANIGARLQIDQAEADKRIAQAKAEQRRAMAVAREQEMKAYTQEMRAKV
                     VEAEAEIPKAIAQALREGKLGVFDYYNLRNIQADTSMRESFSKMTPPSEGEQK"
     gene            complement(1092..2375)
                     /locus_tag="P5310_04060"
     CDS             complement(1092..2375)
                     /locus_tag="P5310_04060"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HRU32569.1"
                     /translation="MKKVLISFILIILAILGSNSAYSNNDTVHIIHISGMINEGVYTL
                     VRRGINKAIENNARYIVVEIDTFGGLVDSAVKIRDALLDSPITTVAYVKNRAWSAGAL
                     IAIACEKIAFTEAGSMGAAEPRPTDEKTVSALRAEFSATAERRKRDPKIAEAMVDQSI
                     EIEGLKPKGKLLTLTASQAKKYKYADLIANSYDELWNELKIKNPRVMEEYLNWSERLS
                     QITADPTYSFLFLLVGMILLLAEFQVPGFGILGALGITSIALTFIGNYLAGLAGIESI
                     LLLIGGLILLFIELHTPGFGIPGIAGIISLFTSLYMVLGGGINATRSIAMAGILILGV
                     IILFITYLPKSFAWKRLALKEAEKTETGYTAVTVEENLVGREGIAVTLLRPAGVVEID
                     NKKYDCLTEGEYIPAGTKVTVTKVIGNRIFVRKID"
     gene            complement(2411..3223)
                     /locus_tag="P5310_04065"
     CDS             complement(2411..3223)
                     /locus_tag="P5310_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006772716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRU32570.1"
                     /translation="MEIMFLGTAAAEGWPGVFCSCDYCKKARELGGKNIRTRSSVLIE
                     KDILVDIPPDTYMHGLRYNIDLSQIKHLFVTHSHQDHFYPQELHMRADPFAHIPEEAI
                     LNIYGNSKVTKIIDDHINIERAKIRLHTAHLFETLGEEGFTLTPLPADHAREEECFLY
                     LINIEGKTIFYGHDSGWYPEKTWEYLSGIYINLAIFDCTFGKNDERNGHMGIPAIISA
                     KERLEKFGSIDSNSISIATHFSHNGGLLHEELEDELSRYGFIVAYDGMVLNI"
     gene            3305..3601
                     /locus_tag="P5310_04070"
     CDS             3305..3601
                     /locus_tag="P5310_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32571.1"
                     /translation="MEKLSLDGEWLFKSDSDSEWLPALVPGDVHMDLLRNKKIDDPLY
                     GTNAEKCRWIEEKIWMYKKIFNIDKNFIHKRVELVFNGLDLDGEIFLNGIKTKT"
BASE COUNT          955 a          839 c          599 g         1228 t
ORIGIN      
        1 ctttttattc tctcttatcc ctcttatctc ttctttatac tctaggctta tcccctcttg
       61 agtttcatcc tgggttattt ctttactcat ttctgttcac cttctgatgg tggggtcatc
      121 tttgagaaag attctctcat agatgtgtcc gcctgaatat ttctgagatt ataatagtcg
      181 aatacaccaa gtttaccttc tctaagtgcc tgagctatag cttttggaat ctctgcctct
      241 gcctccacca ctttagccct catctcctgg gtatatgcct tcatctcctg ttctctggct
      301 accgccatag ctcttctctg ttccgccttt gcctgggcta tccttttatc agcctcagcc
      361 tggtctatct gaagccttgc tcctatatta gcccccacgt ccacatctgc tatatctata
      421 gataatatct cgaatgctgt tccggcgtct agccccttct ccaggacagt cctggatatc
      481 ttatctggat tttccaggac ctgtttgtgg gtttctgaag acccgatggt ggtaactata
      541 ccttctccaa ctctggctat tatggttgcc tctccagctc ctcctacaag cctttctata
      601 ttagctctta ccgtaactct tgcttttacc ttcagttcta tcccatcttt agctaccgct
      661 gctactatag gggtctctat tacctttgga tttacactca tctgaacagc ttccaggaca
      721 tctctccccg ctaaatctat agctgctgct ctggtaaaat ttagcgggat gccagctctt
      781 tctgcagcaa taatggcatc tacaactcta tccacatttc ctcccgccag ataatgagct
      841 tccagctgat ttatgtttaa atccaatcct gcttttgttg cttttataag aggtaacact
      901 atcttatggg gtggaaccct ccttaacctc atccctataa gtgttactat tgtgactttt
      961 actgatgctg ctatggcaga tatccatagt cctaaaggga caaattggaa aaaaagtatc
     1021 aatgctataa ctattacaac tattggtaac caataggcaa taactgtgtt ttccataaaa
     1081 accacctcct cctaatcgat ttttcttaca aaaattctat taccaatcac tttagttact
     1141 gttacctttg ttccagctgg tatatattct ccctctgtaa gacaatcata ttttttatta
     1201 tctatctcca caaccccggc tggtcttaag agagttaccg ctattccttc tctacccacc
     1261 agattctctt caactgtaac tgcagtataa cctgtttctg ttttttcagc ctcctttaag
     1321 gctaatctct tccatgcgaa acttttaggg agatatgtaa taaaaagtat tattactccc
     1381 agtatgagta tacctgccat tgcaatagac ctggtcgcat tgatcccgcc acccaatacc
     1441 atatataaac ttgtgaatag tgaaattatc ccagctatac caggtatacc aaacccagga
     1501 gtatgtagct ctataaagag aagtataagc cctcctatca gaagtagtat ggactctata
     1561 cctgctaaac cagcaagata gttccctata aatgtaaggg cgatagaagt tatccctaat
     1621 gccccaagta taccaaatcc tggaacctgg aattctgcaa gtagcaggat cattcctaca
     1681 aggagaaata aaaaactgta ggtgggatct gccgttatct gtgaaagtct ttctgaccag
     1741 ttcagatatt cttccattac tctggggttc tttatcttaa gctcattcca gagttcatcg
     1801 taactattgg ctataaggtc tgcatactta tatttcttag cctgactggc ggtaagggtt
     1861 agcaatttac cttttggttt tagcccttct atctctatac tttgatcaac catagcctca
     1921 gctattttcg ggtctctttt tctcctctct gccgtagcag aaaattcagc cctcaatgct
     1981 gacacggttt tttcatcggt aggacgtggc tcagctgcac ccatagaacc agcctcagta
     2041 aatgcaatct tctcacaggc tatagctatc aatgctccag cagaccatgc cctgtttttt
     2101 acataggcta ctgtagttat tggactatca agcagtgcat cccttatttt caccgcagag
     2161 tccacaagtc ctccaaaggt atctatctct accactatat acctggcatt attctctata
     2221 gccttgttta tccctctacg gacaagcgta tatacccctt cgtttatcat ccctgagata
     2281 tggatgatat ggacagtatc gttattgctg tatgcactgt tacttcccag aatagcaagg
     2341 attatcagta taaaagatat aagtaccttc ttcaactcta cctcctctgt ttacattata
     2401 ccatttttat ttatatattt aagaccatac catcgtatgc aactataaat ccatatctgc
     2461 ttaactcatc ttccagctct tcatgtaata atcctccatt atgagaaaaa tgagtcgcta
     2521 tacttatgct gttggaatct atacttccaa acttttctaa tctctctttt gcagaaatga
     2581 ttgctggtat ccccatgtgc ccatttcttt catcgttctt accaaatgtg cagtcaaaga
     2641 ttgcaagatt tatgtagata cctgatagat actcccacgt cttttcagga taccatccag
     2701 aatcatgtcc ataaaatata gtttttcctt cgatatttat cagatacaag aaacactcct
     2761 cttcccgtgc atggtctgct ggaagaggag tcagagtaaa accttcttcc cctaatgttt
     2821 caaaaaggtg agctgtgtgc aaccttatct ttgctctttc aatatttata tggtcatcta
     2881 ttatttttgt cactttggaa ttaccatata tatttaagat tgcttcttct ggtatatgtg
     2941 cgaatggatc agctctcata tgtaattcct gggggtagaa gtggtcctga tgagagtggg
     3001 taacaaatag atgtttaatt tgactcaggt ctatattgta tctgagacca tgcatatagg
     3061 tatctggtgg tatatctacg agaatatctt tctctatgag aacagatgac cttgtcctta
     3121 tattcttccc tccaagttcc ctggctttct tacaataatc gcaactacag aatacccctg
     3181 gccacccctc agcagcagct gtcccaagaa acattatttc catatctatt cctcctatct
     3241 tgacacatat ttagtttatt ctaaaataat agcataaatc ttgttgttct ttggaggtga
     3301 caggatggag aaattatctt tagatggaga atggttattt aaatcagatt cagatagtga
     3361 gtggttacca gctttagtcc caggagatgt tcatatggac cttctgagaa ataagaaaat
     3421 agatgaccct ctctatggta ccaatgcgga aaagtgcagg tggattgaag agaagatttg
     3481 gatgtacaaa aagatattca acatagataa aaatttcatt cataagagag tagaacttgt
     3541 atttaacggg cttgatttgg atggggagat ttttcttaat gggataaaaa ctaaaaccta
     3601 acatctcttt gttttcttca a
//
LOCUS       DAOTKU010000129         3617 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_21476_length_3617_cov_3.463504, whole genome shotgun sequence.
ACCESSION   DAOTKU010000129 DAOTKU010000000
VERSION     DAOTKU010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3617
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21476_length_3617_cov_3.463504"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..161)
                     /locus_tag="P5310_04075"
     tRNA            complement(86..161)
                     /locus_tag="P5310_04075"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(126..128),aa:Thr,seq:ggt)
     gene            complement(168..243)
                     /locus_tag="P5310_04080"
     tRNA            complement(168..243)
                     /locus_tag="P5310_04080"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(208..210),aa:Gly,seq:tcc)
     gene            complement(246..331)
                     /locus_tag="P5310_04085"
     tRNA            complement(246..331)
                     /locus_tag="P5310_04085"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(295..297),aa:Tyr,seq:gta)
     gene            complement(334..409)
                     /locus_tag="P5310_04090"
     tRNA            complement(334..409)
                     /locus_tag="P5310_04090"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(374..376),aa:Thr,seq:tgt)
     gene            complement(526..1848)
                     /locus_tag="P5310_04095"
     CDS             complement(526..1848)
                     /locus_tag="P5310_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRU32572.1"
                     /translation="MGEKKVNVLVFFTDQQRWDTVGVYGSAMNLTPNLDALAREGTKF
                     DLAFTNQPVCAPARACIFTGQYATTHKVWRNGFGLKAGDNTLAHCFKKAGYEVGYIGK
                     WHLSPDELGPGYVPSEYRGGFEDYWEAANLLEFTSHPYDTNLYDRDGNLIHISGYRVD
                     ALTERVIKFINQSHSNPFFLVVSYLEPHHQNDLNRFVAPDGYREKFSNPFVPYDLRTF
                     PGDWQEQLPDYYGCIARLDEDFGSIVDTLKEKGMFEDTIVVFLSDHGCHFRTRNTEYK
                     RSCHESSIRVPLIFHGPGFNKSLVIPEPVSLINFAPTILDAVNIPVPDTMQGGSAMPL
                     LDRKIKDWKNEIFIQISESCVARAIRTERWKYSVIAEDKDGWLDSNSDTYTDYQLYDL
                     YSDPYELVNLAGRREYKGIIEELRERLTAKIEEVEGVKPGILERRFYP"
     gene            complement(1863..3293)
                     /locus_tag="P5310_04100"
     CDS             complement(1863..3293)
                     /locus_tag="P5310_04100"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HRU32573.1"
                     /translation="MNVILVVSDTLRRDFLGCYGNSWIHTENIDKFAKDAVIFDNAYA
                     GSFPTVPNRLDLFTGKFTFTYRDWAPLPQNEVVLAEVLSKSGYVTMLIADTPHILKDG
                     YNFQRGFYGWEWIRGQENDRHSTDPVDIKFPCDPKKLRNPYDAVVHYLKNTYWRRHES
                     DYFVAQTMDTAIRWLERNYKQDRFFLYVDTFDPHEPWDPPRWYVDMYDPGYEGEEIIY
                     PRYGPADYLTEKELKHMRALYAGEVTLVDRWVGRLLAKIEDLELFDNTAIIFTTDHGF
                     YHGEHNLTGKSIIIDEKNYHGLAPLYEEVAHIPLIVRLPDTDKSWRCSTFVQPPDIMP
                     TILDLLRIEDTGTMHGKSFLPVIKGDVKSIREFAVTSPSIIYGPVAGQRITVTTDEWR
                     LIYAGNPEEIRGSAVTSIVDGIPREQHPLGEIRSELYNIKEDPQERINLIDKYPDVAT
                     DIHSKLLDFLKSTGTQEKYLRFWDRI"
     gene            complement(3368..>3617)
                     /locus_tag="P5310_04105"
     CDS             complement(3368..>3617)
                     /locus_tag="P5310_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRU32574.1"
                     /translation="KRALKLWETVQTTLDEKEAISAGKEMLKLFVENLWGIGTVGLQP
                     WPVIVKNNLVNVPEGGLLAWDWVYLARYHPEQFFLKGK"
BASE COUNT         1025 a          872 c          609 g         1111 t
ORIGIN      
        1 atttatatgg ggcttggtcc tttcaaactt ctgcttagcc atatttactt atcttcctcc
       61 tacagttatt tacttaggtt atctatggag cccacaaccg ggatcgaacc ggtgacctta
      121 tccttaccaa ggatacgctc tacctactga gctatgtggg ctctaattgg agcgggaaac
      181 gggactcgaa cccgcgacac ccagcttgga aggctggcgc tctaccaact gagctattcc
      241 cgctctggtg gggagggaga gattcgaact cccgaaggcg ggcgccaact gatttacagt
      301 cagtcccctt tagccacttg ggtacctccc cagtggagcc gacgatccga cttgaacgga
      361 caaccagctg attacaaatc agccgctctg ccggttgagc tacgtcggct caatgaactg
      421 atactaatta tacattaaga atctgctaaa tgcaataggc ttatgaaaaa aatcttccat
      481 ggcaaagtca tctatagaat aagattcata tctccctctt tctctctatg gatagaatct
      541 tctctccaat attcctggtt ttacaccttc aacttcttct atcttagctg ttaatctttc
      601 tcttaactct tctattatac ccttatactc tctcctgcct gcaagattca caagttcata
      661 tgggtcagag tagaggtcgt ataactgata atcagtatat gtatcactat ttgaatccag
      721 ccatccgtct ttatcctctg caattacaga gtatttccac ctctcagtcc ttatagcacg
      781 ggctacacag gattcactta tctgtataaa aatttcgttc ttccaatcct taatctttct
      841 atccagtaat ggcatagcac ttcctccctg catagtatct ggaacaggaa tatttacagc
      901 atccagaatg gttggagcaa aatttattaa acttacaggc tcagggataa caagagattt
      961 gttaaatccg ggaccgtgga atattaaagg tactcttata gaagattcgt gacaacttct
     1021 cttgtactcg gtatttctag tcctgaaatg acaaccatgg tctgagagaa acactactat
     1081 agtatcttca aacatccctt tttctttaag ggtatctact atactgccaa aatcctcatc
     1141 taacctggca atacatccat aataatccgg gagctgttcc tgccagtctc ctggaaaagt
     1201 tctgagatca tacggaacaa atgggttaga gaacttttct ctataaccat ccggggcaac
     1261 aaatctattc aggtcattct ggtgatgagg ctcaagatat gagacgacaa gaaaaaaagg
     1321 attactgtga gactgattaa taaattttat aaccctctct gttaaggcat ctaccctata
     1381 tccagatata tgtataagat ttccatctct atcgtagaga tttgtatcat atggatgaga
     1441 tgtaaactct aaaagatttg cagcctccca gtagtcttcg aatcctcccc tgtattctga
     1501 aggaacatat ccaggaccaa gctcgtcagg agaaagatgc cactttccta tatatccaac
     1561 ttcataaccc gccttcttaa aacaatgggc taaagtatta tctcctgctt taagcccaaa
     1621 accatttcgc cataccttat gtgtagtagc atattgaccg gtaaagatac aagctctggc
     1681 tggtgcacac actggttgat tggtaaaggc aagatcaaac ttagttccct ctctagctaa
     1741 agcatcaaga tttggggtca gattcatagc acttccatac acacctactg tatcccacct
     1801 ctgttggtct gtaaaaaata ccagaacatt tactttcttc tctcccatat atcctccctg
     1861 acttatatcc tgtcccagaa tctcaaatat ttctcctgag ttcctgtgga tttaagaaaa
     1921 tccaacaact tagaatgaat atctgtagcg acatccggat acttatctat aagatttatt
     1981 ctctcctgag ggtcttcctt tatattgtac agttcagacc tgatctctcc tagtggatgc
     2041 tgttctcttg gtataccatc aactatactt gtaacagccg accctctaat ctcttctgga
     2101 tttccagcat atataagtct ccactcgtct gtagttactg ttattctctg ccctgctacc
     2161 ggtccataga taatgctggg cgatgtaaca gcaaactctc ttatggattt aacatctccc
     2221 tttattaccg gtagaaaact cttaccatgc atggtcccgg tatcttctat cctgagcagg
     2281 tccagaattg taggcattat atctggtggc tgtacaaaag tactgcatct ccagctctta
     2341 tcagtatcgg gtagtctaac tatcagaggt atatgagcta cctcttcgta tagtggagcc
     2401 agtccatgat agtttttctc atctattata atactcttac cggttaggtt atgctccccg
     2461 tgataaaaac catggtcagt tgtaaatata atagcggtat tatcaaacag ttcaagatct
     2521 tctatcttgg ctaagagtct tcccacccat ctgtctacca gtgtaacctc tccagcatag
     2581 agagccctca tatgtttgag ctctttttca gtaagataat cagctggacc ataccttgga
     2641 tagattatct cctctccttc atatcctgga tcatacatat ccacatacca tcttggtggg
     2701 tcccagggct catgagggtc aaaggtatcc acatagagga agaatctatc ctgcttataa
     2761 tttctctcaa gccaccttat agcagtatcc atcgtctgag caacaaaata atcagactca
     2821 tgccttctcc agtacgtatt cttcagataa tgcaccaccg catcgtacgg atttctcaat
     2881 tttttagggt cacagggaaa ttttatatct acaggatcgg tagaatgcct atcattctcc
     2941 tgacctctta tccactccca tccataaaaa cctctctgaa agttgtaacc atccttcaaa
     3001 atatgaggtg tatcagctat aagcatcgtt acataacctg atttactgag aacttcagcc
     3061 agtaccactt cattttgtgg tagtggtgcc caatccctgt aggtaaaagt gaatttccct
     3121 gtgaaaaggt ccaatctatt aggtaccgtt gggaaagacc ctgcataagc attatcaaag
     3181 ataacagcat ctttagcaaa cttatcaata ttctccgtat ggatccaact atttccataa
     3241 catcctaaaa aatctcttct aagagtatca gaaacaacca gaataacatt catctaaaat
     3301 tcctcctctt acagacatta tgaaggggat aagagacaat gaaatcttct tatccccaat
     3361 tacattttta cttccccttc aagaagaact gctctggatg atatctggca agatataccc
     3421 aatcccatgc caataaacca ccttctggaa cattcactaa attattcttg actattacag
     3481 gccatggctg aagtcctacc gtaccaattc cccagagatt ttctacaaag agttttaaca
     3541 tttccttccc agcacttata gcttctttct catctaatgt cgtctgaact gtctcccaga
     3601 gtttaagagc tctttta
//
LOCUS       DAOTKU010000130         3606 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_21561_length_3606_cov_3.473388, whole genome shotgun sequence.
ACCESSION   DAOTKU010000130 DAOTKU010000000
VERSION     DAOTKU010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3606
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21561_length_3606_cov_3.473388"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="P5310_04110"
     CDS             <1..545
                     /locus_tag="P5310_04110"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRU32575.1"
                     /translation="GRDNIYPVATYDPRAYPRDIEYLEDLISRGFLAIAFFPRMQGWT
                     VDFAPFRMVLDIIEAESFPVVIHTGGYGEITKISILAEEIENPVIISGTGYSNLSEAI
                     AVAQEVDNIYIETHLLNSPDALEFCRRYIGIERFVFGSGAPVLSFETAYNVVEKSSLS
                     DDDKEMIFYKNLEDLVGVRL"
     gene            542..1279
                     /locus_tag="P5310_04115"
     CDS             542..1279
                     /locus_tag="P5310_04115"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRU32576.1"
                     /translation="MIIDIHCHYGLWSFAIDSFEAEKVKEYMDRFDVKRAVFSSAKGI
                     VYDFREGNRDLMNFLEEDDRFLGYVVLNPNYLEESIEEVERYKENNKIVGIKLHPSYS
                     RVPISDEKTFKIVEYFSNTKLIYLIHTWGVSMSTQLARLAEKFPDVRFIMAHTGGDTW
                     SGCLDVVRPYPNIYVEPCSSYADRDKLRVSIDTVGINRVLFGSDFTLINPAFVIGMVE
                     GGGLTVEEKQTVYYKNAIKLLGGDIDA"
     gene            1272..2105
                     /locus_tag="P5310_04120"
     CDS             1272..2105
                     /locus_tag="P5310_04120"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HRU32577.1"
                     /translation="MPEIGALGLSMGRYTFEEFLQYASENGFDCIEISTSPGVHKNSM
                     PMTFENIPEIKKLLAKYNLKPFAVSGYNDFVQKDTEELARQIEKLENTCKLAREVSAK
                     VVRAFGGEVKEGINKKDTVKLIIEGFKEATKRAEKLGIILGLENHGEITNDARVELDI
                     LEGVGSDYLRLTVDTSNYYWYGNKLEDIEEFFKLVAPYTVHTHLKDGSTKNGIRDNYI
                     SLALGEGEINLKLFLTELKDNGYSNPLCIEYEGIEDPKIGLKKGFIWLKNTLKEIGWE
                     V"
     gene            2108..3307
                     /locus_tag="P5310_04125"
     CDS             2108..3307
                     /locus_tag="P5310_04125"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRU32578.1"
                     /translation="MKLAIKGGNPVRTKPFTKWPVFDEREENALIEVLRSGEWGRISG
                     KKNEEFEEKFSQFVGARFGVTCVNGTAALEIALRAMGIGRGDEVIVPAYTFIATASAC
                     ILVGAEPVFVDIDPDTYNIDPKQIESAITAKTKAIIPVHIGGCPANMDEIMRIARKYN
                     LLVLEDSAQAHGAEWKGAKVGSIGDMATFSFQASKNINAGEGGIVTTNNKKFADMAWS
                     LMNVGRVKEGGWYDHPILGWNYRMTEFQAAILLVQLERIEEQMKKRERNAGYLAEGLK
                     RIGGVEPLVPDSGVTRNAYHLFIFKYNKNEFKGVKRDKFVKALNAEGIPASTGYVPLY
                     KFPAILDWPYLERNYRDVYLPVTEKACYEEGIWLPQNVLLGPREDMDDILSAVTKVKE
                     NIEELID"
     gene            3304..>3606
                     /locus_tag="P5310_04130"
     CDS             3304..>3606
                     /locus_tag="P5310_04130"
                     /inference="COORDINATES: protein motif:HMM:NF024241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRU32579.1"
                     /translation="MRGLKINNEAIYFLIGFSLFLGSGATQPFIIPHLEGLGYSSLVS
                     SLVIAFIYISQIPTRFFVNKFEKFLGQVPVVFIGIAGYSLYAIILAFSTQLWQFLMA"
BASE COUNT         1152 a          558 c          886 g         1010 t
ORIGIN      
        1 tcggcaggga taatatatat cctgttgcca cctatgatcc aagggcttat ccaagagaca
       61 ttgaatatct tgaggattta atctccagag gatttctcgc catagcattt ttccctcgta
      121 tgcagggctg gaccgtggat tttgctccat tcaggatggt cctggatatc atagaggctg
      181 aatcttttcc tgtagttatt catacaggag gttatggaga gataacaaag atatctatac
      241 tggctgaaga gatagaaaat cctgttataa tatcaggtac agggtactca aatctttctg
      301 aggctatagc ggtagctcag gaggtagata atatatacat agaaacccat ctcttaaaca
      361 gcccggatgc cctggaattt tgcaggaggt atataggtat tgaaagattt gtatttggtt
      421 ctggtgctcc agtgttatcg tttgagaccg cctataatgt tgtggaaaaa agctcactct
      481 cagacgacga taaggaaatg atattctata aaaacttaga ggatttagta ggagtaagat
      541 tatgattata gacatacatt gtcattatgg tctctggtcg tttgccattg atagttttga
      601 ggcagagaag gttaaggagt atatggatag gtttgatgta aaaagagcgg tattctcttc
      661 agcaaaaggc atagtctatg actttagaga gggtaacaga gatttaatga actttttaga
      721 agaagatgat agattcctcg gctatgtggt tctgaaccca aattatcttg aagagtccat
      781 cgaagaggta gaaagatata aagaaaataa taagattgtt ggtataaaac ttcatccatc
      841 ttattccaga gtcccaatca gtgatgagaa aacatttaag atagttgaat acttttcaaa
      901 cacgaagctt atatatctta tacatacctg gggcgtatct atgtctactc agctggcaag
      961 gttagcagaa aagttccctg atgttcgatt tattatggcg cataccggcg gcgatacctg
     1021 gtccggatgt ttagatgtgg taagaccata tcccaatata tatgttgaac cctgttctag
     1081 ttatgctgac agggataaat taagggtcag catagataca gtgggtatta atagagttct
     1141 ttttggctca gattttaccc tcataaatcc agcttttgtt attggtatgg tagaaggtgg
     1201 tggacttaca gtggaagaga aacagacagt gtactataaa aatgctataa aacttttagg
     1261 aggtgatata gatgcctgaa ataggagcct taggtttatc tatgggaagg tatacgtttg
     1321 aggagttttt acagtatgcc agtgagaatg gttttgactg tatagagatt tccacatcgc
     1381 ctggcgtcca taaaaacagt atgcctatga cgtttgagaa tataccggag ataaagaaac
     1441 tgctagctaa atataatctt aagccctttg ctgtctctgg ttacaacgat tttgtccaga
     1501 aagatacaga ggaattggca agacagatag aaaaactgga aaatacctgt aagttagcca
     1561 gagaagtctc agctaaagtt gttagggctt ttggtgggga agtgaaggaa gggataaata
     1621 agaaagatac tgtaaagctg ataatagaag gatttaaaga ggcaactaag agggctgaaa
     1681 aactcggaat aatcttaggg ctggagaacc atggtgagat aaccaatgat gccagagttg
     1741 agcttgatat ccttgaaggg gttggctcgg attatctgag gcttacagtg gatacgtcta
     1801 actattactg gtatggtaat aagcttgagg atatagaaga gttttttaag ttagttgctc
     1861 catacactgt tcatacccat ctgaaagatg ggagtacaaa aaatggaatc agggataatt
     1921 atatatcgct agcccttgga gagggagaga taaatttaaa attattcctt acagaactta
     1981 aagataatgg ttacagtaat ccactctgta tagagtatga ggggattgaa gatccaaaga
     2041 ttggtttaaa gaagggtttt atctggttaa agaatacatt aaaagagatt ggatgggagg
     2101 tatagatatg aagttggcta taaaaggtgg taatcctgtc aggacaaagc catttacaaa
     2161 gtggcctgta tttgatgaga gagaagagaa tgcgttaata gaggtcttaa ggagtggaga
     2221 atggggtaga atatctggca aaaagaatga agagtttgag gaaaagttca gtcagtttgt
     2281 tggagccaga tttggtgtta cctgtgtaaa tggaacagcc gctctggaga tagctttaag
     2341 agctatgggg ataggcagag gggatgaggt gattgtacca gcatatacct ttatagctac
     2401 tgcaagtgcc tgtatactgg taggagcaga acccgttttt gttgatatag accctgatac
     2461 atataatata gacccaaaac agatagaatc tgctataaca gcaaaaacaa aggctataat
     2521 tcctgttcat ataggtggct gccctgccaa tatggatgag attatgagga tagcaaggaa
     2581 gtataacctc ctcgtgctgg aggattctgc ccaagcccat ggggcagagt ggaaaggggc
     2641 aaaggttggg tctataggag atatggctac cttcagtttc caggcgtcaa agaatataaa
     2701 tgcaggagaa ggtggaatcg ttacaactaa caataagaag tttgccgata tggcatggtc
     2761 actgatgaat gtcggaaggg taaaagaagg aggatggtat gaccatccga tcctgggatg
     2821 gaactataga atgacagagt ttcaggctgc tatacttctc gtccagctcg agaggataga
     2881 agaacagatg aaaaaaagag agagaaatgc cggatatctg gcagagggac tgaagaggat
     2941 aggtggagtt gaaccgctgg ttccggattc tggagttaca aggaacgcat accatctctt
     3001 tatattcaaa tacaataaga atgaattcaa gggggtaaag agagataagt ttgtaaaggc
     3061 attaaatgca gagggcattc cagccagcac aggatacgtc ccactttata agttccctgc
     3121 catattggat tggccatatc tggagagaaa ctacagggat gtatatcttc ctgtaactga
     3181 gaaggcatgc tacgaagagg ggatatggtt acctcagaat gtcttattgg gtcccaggga
     3241 agatatggac gatatcctgt cagctgtaac caaagtaaag gagaatatag aggaactgat
     3301 agattgaggg gacttaaaat taacaacgaa gccatttact ttttaatagg cttctccctt
     3361 tttttaggct caggagccac tcagccattt ataataccac acctggaggg tctgggatat
     3421 tcatcactgg taagtagcct ggtaatagct tttatataca tatcccagat cccaaccaga
     3481 tttttcgtca ataaatttga gaaatttctc ggacaggttc ctgtagtatt tatcggtatt
     3541 gctggatact ctctctatgc aatcatcttg gcatttagta cccagctctg gcaattttta
     3601 atggca
//
LOCUS       DAOTKU010000131         3595 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_21629_length_3595_cov_3.761299, whole genome shotgun sequence.
ACCESSION   DAOTKU010000131 DAOTKU010000000
VERSION     DAOTKU010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3595
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21629_length_3595_cov_3.761299"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1076)
                     /locus_tag="P5310_04135"
     CDS             complement(<1..1076)
                     /locus_tag="P5310_04135"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HRU32580.1"
                     /translation="MEKLEHAIARLELRLGELNTFKLVKRFNLDEWLFSTDKSTWKTI
                     HIGEKWTTQSFPCYFKKEFSIPEEFKGLPVRLQIWVEGEGIVYINDKPVGGLDPNHRE
                     FRLLDSASGGEKFSILIEAVPRGPFGSYVADPHLAQSRLVSIDESVESAIFDIYAVLD
                     AAKNLSDENELKEILVEALDNALSLVVFPSETESYLTSVTENPFGSRMVFRVWNPPVP
                     KGDFVSPEETRKSLVSAQKKLQELLEEIKDQYPPVGNITLTGHSHIDLAWLWPMAETR
                     RKIRRTSSSVISLMNKYSDFKYNQSSAQIYAFLKEDEPYLYEKAKERIKAGQWEPIGG
                     MWVESDCNLTSGESLVRQMLYAQK"
     gene            complement(1082..2284)
                     /locus_tag="P5310_04140"
     CDS             complement(1082..2284)
                     /locus_tag="P5310_04140"
                     /inference="COORDINATES: protein motif:HMM:NF016083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF401 family protein"
                     /protein_id="HRU32581.1"
                     /translation="MSALLKISIAFIVVVFLLKRKVKLGYCLLAGSLLLGIFFRIQPD
                     GYLRFTLLAFEDPANQKLILTLFFILVLANVMGETGQLERIVLLAKSVIKNPKIVIAI
                     MPAFIGFLPIPGGALFSAPLVEKTDMDSEISPPMRSFINYWFRHVWEYFFPLYPEVIL
                     AVSITGMKMSEFIVMQLPLSIVAIAIGFIAMSRIRLSNNTVEDSSIGDWKTLVLYTLP
                     ILLPIILILLGLASWLSIMIGILFALLINKKDSLSIVSGILKRFPWDVILNVVGVVIF
                     KSFIENTGGIPQIVQFFTQHNLPFILVVTIAPFLVGLFSGMGIVPIGVTFPLIIPFTT
                     PANFNTSIMLAFACGFMGVMLSPAHLCLVVSNQYFKADVEKVYRLLAAPVMSIILTAL
                     LIFYVKGG"
     gene            complement(2304..3263)
                     /locus_tag="P5310_04145"
     CDS             complement(2304..3263)
                     /locus_tag="P5310_04145"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HRU32582.1"
                     /translation="MELFRYVFIFILSLLGTYFLTPVFRRLAIKIDLLDRPNPRKIHS
                     EPIPIAGGFGFLFIFITLSLIFLPVDRKLLSIIGAVLVIFLTHLVDDLFKSKSKDFPA
                     LPKFLGQVTGALILISGGVSISVIKNPFTGGYLQFSTPVSTILTVVWVVGVSNIINFA
                     DGLDGLASSITAIASGSLAIVSHFLNRDYATTMAVILLGISLGFIRHNMSSTNKVFMG
                     DSGAYLLGFILGTIAIEGTMKTTTAIAVLVPFIILGQPIIDTGLKFIKKTIRREPFYK
                     ADREHLHFKLLNWGLTSTQALIFISLINICFSLVALVIIFAGK"
     gene            complement(3273..>3595)
                     /gene="rplT"
                     /locus_tag="P5310_04150"
     CDS             complement(3273..>3595)
                     /gene="rplT"
                     /locus_tag="P5310_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HRU32583.1"
                     /translation="RHKKIMKLASGYRGARGRRFRTANETLLKALSYSYRDRRARKRE
                     FRRLWIARINAGVHEMGMTYSQFMHGLKLAGVSLNRKVLAELAVSDKEEFRRIVELSR
                     SALK"
BASE COUNT         1110 a          811 c          666 g         1008 t
ORIGIN      
        1 aacttctgag catataacat ctgcctgacc agcgattctc cagatgtaag attgcaatca
       61 ctctccaccc acataccacc tattggctcc cactgtccag cctttatcct ttctttagcc
      121 ttctcataga ggtatggttc atcttccttt agaaaggcat agatctgggc actgctctga
      181 ttatacttaa aatcagaata cttattcata agagagatta cacttgagga tgttcttctt
      241 atctttctcc tagtctcagc catcggccag agccaggcaa gatctatatg tgaatgacct
      301 gtcagcgtaa tattacccac tggtggatac tgatctttta tctcttccag taattcctga
      361 agcttctttt gagcagatac cagactcttt ctggtctctt caggacttac aaaatccccc
      421 ttcgggacag gtggattcca gaccctaaat accatcctgc ttccaaatgg gttctcggta
      481 acacttgtaa gatagctctc agtctcagat gggaatacta caaggcttag agcattatcc
      541 agtgcctcaa ccagaatctc tttcagctca ttctcatcgg agagattttt ggcagcatcc
      601 agaacagcat aaatgtcaaa tatggcggat tccactgact catctatgct gacaagtctg
      661 gattgagcca gatgagggtc tgctacataa ctgccaaaag gtcctcttgg aacagcctca
      721 ataaggatag agaacttttc tcctccgctg gctgaatcaa gaagtctgaa ctcccggtga
      781 tttgggtcca accctcctac aggtttatcg ttaatataga cgataccctc accctctacc
      841 catatctgta acctcaccgg cagtccttta aattcttccg ggatactgaa ctctttctta
      901 aaatagcagg ggaaggattg agtcgtccat ttttctccga tatgtatcgt cttccaggta
      961 ctcttatcgg tactgaataa ccattcatcc agattgaatc tcttaaccag cttaaatgta
     1021 tttaactctc caagccttag ctccaatctg gctattgcat gttctaattt ttccaattct
     1081 attagcctcc tttcacataa aatataagca gggctgtcaa tataatcgac attaccgggg
     1141 ctgcaagaag cctgtatacc ttctccacat cagccttaaa atactgatta ctcacaacga
     1201 gacatagatg tgccggggat aacataacac ccataaatcc acaggcaaat gccagcataa
     1261 tactggtatt aaagtttgcc ggggtggtaa atggaataat tagaggaaag gtgacaccga
     1321 tagggactat acccataccg ctaaaaagac caaccaggaa tggcgctatg gtgactacaa
     1381 gaatgaatgg aaggttgtgc tgtgtgaaga attgaacgat ctggggaatt ccacccgtat
     1441 tctctataaa actcttgaaa ataacaaccc caaccacatt caggattaca tcccatggga
     1501 acctcttcag tattccagaa acaatggaaa gagaatcttt tttattgata agcagggcga
     1561 acaggatacc tatcattatg ctgagccagg atgctagacc caataggata agaattatgg
     1621 gtagaagtat tggaagggta tataagacaa gagttttcca gtcaccaata gaagaatctt
     1681 ccactgtatt attactcaac ctgatcctgc tcatagcgat aaaacctata gcgatagcaa
     1741 ctattgagag aggtaactgc atcacgataa actcagacat cttcattccg gttatactca
     1801 cagccagtat cacctcagga tagaggggga aaaaatactc ccatacatgt ctgaaccagt
     1861 agtttataaa agaacgcatg ggaggactga tttcagaatc catatctgtc ttctcaacca
     1921 gtggtgctga gaatagagct cctccaggta tcggtaaaaa gcctataaat gccggcatga
     1981 ttgctataac tatcttcgga ttttttatca cacttttggc tagaaggact atcctctcga
     2041 gctgtccagt ttcccccatc acgttggcaa gtaccagtat gaagaataat gtaagtatta
     2101 atttctgatt ggcggggtct tcaaacgcca ggagggtaaa tctaagatat ccatctggct
     2161 gaattctgaa aaaaattcca agtaataatg aacctgctaa aagacagtat ccaagtttaa
     2221 ccttacgttt taacaggaat acaacaacta taaaagcaat agaaatcttt aatagagcag
     2281 acatctaata tctaagatgt cacctacttt ccagcaaata ttatcacgag agccacaaga
     2341 gagaaacata tatttattag agagataaaa ataagtgcct gagtactggt cagtccccag
     2401 ttgaggagct taaaatgcaa atgttctcta tcagccttat agaatggctc tcttcgaatg
     2461 gtctttttta taaatttcaa tccggtatct attataggtt gtccaaggat tataaaagga
     2521 acaagaacgg ctatagctgt tgtggtcttc atggtgccct ctatagctat cgtcccgagg
     2581 atgaaaccga gcagataggc gccgctatct cccataaaaa ccttatttgt agaactcata
     2641 ttatgtctta taaatcccag ggaaattcct agaagtataa cagccatagt tgtcgcatag
     2701 tctctattta agaagtggct aactatagcc agagatccac tggctatagc agttatactt
     2761 gatgctaaac catctagccc gtcggcaaaa tttataatgt tactcacccc aaccacccat
     2821 actactgtaa gtatggtgga aacaggagta gagaattgca gataaccccc tgtaaatgga
     2881 ttttttataa cagagatact tacacctccg gagattagaa tcagagctcc agtcacctgc
     2941 cccaggaatt taggaagagc aggaaaatcc ttcgactttg acttaaataa atcgtctacc
     3001 aggtgggtaa ggaatatcac cagcacggca ccaattatac tgagtaattt cctgtctaca
     3061 gggaggaaaa tcagtgaaag tgtaataaat ataaataaaa agccaaatcc cccggctatg
     3121 gggattggct cagaatgaat cttcctggga ttaggtctat ctaaaagatc tatctttata
     3181 gccagacgtc tgaagacagg ggtaaggaaa taggtaccaa gcaaactgag gataaaaata
     3241 aaaacatatc taaagagttc catacccccc tcttatttca gggctgacct ggaaagttct
     3301 actatccttc taaactcttc tttatctgaa accgccagtt cagcaagtac cttacgatta
     3361 agagaaaccc cagccaattt caatccatgc atgaattgag aataagtcat tcccatttca
     3421 tgaacaccag cgtttattct ggctatccat aaccttctaa actctctctt tctggctctt
     3481 ctatccctgt atgaataact gagagccttg aggagagtct catttgcagt tctaaatctc
     3541 ctacctcttg cccctctata cccactggca agtttcatta tcttcttatg tcttt
//
LOCUS       DAOTKU010000132         3541 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_22028_length_3541_cov_3.393287, whole genome shotgun sequence.
ACCESSION   DAOTKU010000132 DAOTKU010000000
VERSION     DAOTKU010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22028_length_3541_cov_3.393287"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1468
                     /locus_tag="P5310_04155"
     CDS             <1..1468
                     /locus_tag="P5310_04155"
                     /inference="COORDINATES: protein motif:HMM:NF019368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HRU32584.1"
                     /translation="QGLFQNAKAGGNMVKELALKAMVGEVDTDAGCKVKNVPILVFNP
                     LPWKRTDLVVADIYYAFGEKDRLSLIDDAGRVIPVQYKEYSFFQKALRATFVAEDIPG
                     YGYKTYYLKYAEDEEITSQVSVTDMYTGQVTESGVRASSKYKLENQFYRLTLNAGLGG
                     ITSIVDKATGKEILPKGGVAGLLQILYEAPHPASAWEIGQIIRTINLDSGATLEIVES
                     GPSRVIVRSRRKYNESEFILDIILSNNVPRIDFRLEIFWLERGSKEIGVPMLKVGFPV
                     DITKGKARFDIPFGSIERIPDGNEVPSQKWVDLTGIDSGGDKMGITLLNNCKYGFDVT
                     ENTIRMTLLRSSYEPDPVPERGKYEFQFSLCPHIGELKPAKLIKLGNEFNYPLVPYVT
                     DIHRGRLDKSIEIINVEPENAMVSIMKKAEDNDDIILRIYETDGVETRAKINLNLSCG
                     SYVETDLLEQPVGEEKSLIGRTIQVTLKPYEIKTLRIK"
     gene            1496..3238
                     /locus_tag="P5310_04160"
     CDS             1496..3238
                     /locus_tag="P5310_04160"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRU32585.1"
                     /translation="MMKKPDIIVLLILISAVTLGFVSARFISDMFIAIIVTVCILCIG
                     GFLAVKSLQSRENISEGYEDTDRTTQKFIDLANHIEEIAREGFTRFIEARDNGDIKKI
                     ADSFNYLLRSTRDFVRELDKMSEDVLGASRELSKAIQSTSDSMEEVNSTLQRLAETTE
                     ELNQNIEEIDEGAKEVGNLTQEGLRYIDGLGKQMKEIIQTAEETASSISELNNVSEEI
                     RKILYVISEIAEQTNLLALNATIEAAHAGEQGRGFAVVASEIRQLASRTQRSLEDIRQ
                     FINRFSEETSRAITSIKNNNRQISDGEKILKDTSDAFSVIASNIEGIVSSISQIARAS
                     GEITSGTREISASTEQQTVTISEISSMANRLAEIAGELKEKLAESQFGVSEIELDLDK
                     YDREIKNLDPKKLESLRDELGIRNKFVISVIARLEPVKGHRFFINGIKQLFTKYKNLA
                     CLIIGDGSLEGELKEIVAREGLKDRIKFLGYRKDIPYILNISDLIVLPSEKEGVPPKI
                     IAEAMAISKPVVATDVRGSRYLVKDGETGMLVRYGDVNKLASAVEFFVKNPEMGKDYG
                     RNGRRRIENLVRLN"
     gene            3336..>3541
                     /locus_tag="P5310_04165"
     CDS             3336..>3541
                     /locus_tag="P5310_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HRU32586.1"
                     /translation="MNSIELLLRAIHNESVERIPWVPFVGCHAGNLIGVTAEEYFKNS
                     DNIIRGAIKAFEEYKPDGLPVTFDL"
BASE COUNT         1233 a          507 c          881 g          920 t
ORIGIN      
        1 ccagggactt ttccagaatg caaaagccgg cgggaatatg gtaaaagagt tagcattaaa
       61 ggcgatggtg ggagaggtag atacagatgc aggctgcaaa gtaaagaatg ttcctatatt
      121 agtttttaat ccattaccct ggaagaggac agatctggtg gtggcagata tatattacgc
      181 cttcggggaa aaagacagac tgtctcttat cgatgatgca ggaagggtta ttcctgttca
      241 gtataaagag tattctttct tccaaaaggc attgagagct acattcgtag cagaagatat
      301 acctggatat gggtataaaa cgtactatct gaaatatgct gaagatgaag agattacttc
      361 acaggtatcg gtaaccgata tgtatactgg acaggtaaca gagagcggag taagggcaag
      421 ctccaaatat aaattagaga atcaattcta tagacttacc ttaaatgcag gtttaggtgg
      481 aataacaagt atagtagata aagctactgg gaaagagata cttcctaaag gtggggtagc
      541 aggtctattg cagatactat atgaagcccc gcaccctgcg agtgcctggg agataggaca
      601 gataataagg acaataaacc tggatagtgg agcaactctg gagatagtag agagtggacc
      661 ttcaagggtt atagtgagga gccgtagaaa gtataacgaa tcagaattta tccttgatat
      721 tatactgagc aataatgttc cgaggataga ttttagactt gagatattct ggcttgaaag
      781 aggaagtaaa gagattggtg ttcctatgtt aaaggtagga tttcctgtag atattacaaa
      841 gggtaaagcg aggtttgata taccatttgg aagtatagaa agaattcctg atggtaatga
      901 ggtcccatct cagaagtggg ttgaccttac cggtatagat agtggtggag ataagatggg
      961 aataactctt ttaaataact gtaagtatgg gtttgatgta actgaaaata cgatcaggat
     1021 gacactgctg agaagttctt atgaaccaga cccggtgcca gaaagaggta aatatgagtt
     1081 tcaattctcc ctgtgtcccc acatagggga actaaaacca gcaaaactta taaaactggg
     1141 taatgaattt aactatccat tggttccata tgtgacagat attcacaggg gcagattgga
     1201 taagtctata gagattataa acgtagagcc tgaaaatgct atggtatcca taatgaaaaa
     1261 ggcagaggat aatgatgata ttatactaag gatatatgaa acagacggag ttgaaacccg
     1321 ggctaagata aacctcaacc tgtcctgtgg atcttatgtt gagaccgatc ttttagaaca
     1381 gcctgtggga gaagaaaaat cattaatcgg gagaaccatc caggtaactc ttaaacctta
     1441 tgagataaag acattgagga taaaataggg ttgtattgca cttaggagat agaaaatgat
     1501 gaagaagcct gatattatcg tattattgat ccttatatca gctgttaccc ttggttttgt
     1561 ctccgccagg tttatctcag atatgtttat tgcaattata gttaccgtat gtattctctg
     1621 tatcgggggc tttcttgcag tcaaaagttt gcagagcaga gagaatatat cggaaggcta
     1681 tgaagatacg gatagaacaa ctcagaaatt tatagatcta gccaatcata tagaagagat
     1741 agccagggaa ggttttacca gatttataga ggcaagggat aatggagata taaagaagat
     1801 agcagactct tttaactatc tgttgagaag taccagagat tttgtcagag aactggacaa
     1861 aatgtcagaa gatgtgcttg gggcaagcag agagctgagt aaagctattc aaagtacatc
     1921 ggactctatg gaagaggtaa atagtacatt acagagactg gcagagacaa ctgaggaatt
     1981 gaaccagaat attgaagaga tagatgaagg ggcaaaagag gtaggtaatc taacccagga
     2041 agggctccgt tatatagatg ggttggggaa gcagatgaag gaaataatac agacggcaga
     2101 agagactgcc agcagtatat cggaattaaa taatgtctct gaagagataa ggaagatact
     2161 ctatgttata tctgagatag ccgaacagac aaatctgctg gcgttaaatg ccaccataga
     2221 agcagctcat gctggagagc agggaagagg ttttgctgtg gtagccagtg agataagaca
     2281 actggcatca cgcacacagc gatctctgga agacataagg cagtttataa atagattctc
     2341 cgaagagacc tcccgcgcaa ttacttctat aaagaacaat aacagacaga tttctgatgg
     2401 agaaaagatt ttaaaagata catctgatgc tttcagcgtg atagcctcta atatagaggg
     2461 tatagtttca agtataagtc aaattgctag ggctagtgga gagataactt ctggaaccag
     2521 agagatatca gcttctacag aacagcagac ggttaccata tctgaaataa gtagtatggc
     2581 aaatcgttta gctgagatag ctggtgaatt aaaggagaaa ctggcagaat cccagtttgg
     2641 ggtctctgag atagaactgg atttagacaa atatgatagg gagataaaaa acctcgaccc
     2701 gaagaaatta gaaagtctca gagatgagct gggtataaga aataaatttg ttataagtgt
     2761 tatagccagg ttagagcctg taaaaggaca tagatttttt ataaatggga taaaacaatt
     2821 atttacgaaa tataaaaacc tggcatgcct gataatagga gacggttctt tagaaggaga
     2881 gcttaaagag attgtggcaa gggaagggct aaaagatagg ataaaattcc tcggttatag
     2941 gaaggatata ccatatatac tgaacatatc ggacctgatt gtccttccct cagaaaaaga
     3001 gggtgttcca ccaaagatca tagccgaggc tatggctata tcgaaaccag ttgtagctac
     3061 agatgtgagg ggaagtagat atttggtgaa agatggagaa acaggtatgc tggtcaggta
     3121 tggagatgtg aacaaactgg cgagtgctgt agagttcttt gtaaaaaatc cagagatggg
     3181 taaagattat ggtagaaatg ggagaaggcg tatagagaat cttgtaagac ttaattaaga
     3241 gagtattgct tgaatttctt gacagtaggg agaggtaacg tgtaaaatta taagaaactt
     3301 tatatacttg gtaaagtttt taatgaggtg agctaatgaa ttcaatagaa ctattgttaa
     3361 gagctataca caatgaatcg gtggaaagga taccgtgggt tccattcgtg ggttgtcatg
     3421 ctggaaacct tataggtgtt acagcagaag aatattttaa aaacagtgat aatattataa
     3481 gaggagccat taaggctttc gaagaatata aaccagatgg gttacctgtg acttttgacc
     3541 t
//
LOCUS       DAOTKU010000133         3510 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_22289_length_3510_cov_4.815919, whole genome shotgun sequence.
ACCESSION   DAOTKU010000133 DAOTKU010000000
VERSION     DAOTKU010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22289_length_3510_cov_4.815919"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1520
                     /locus_tag="P5310_04170"
     CDS             <1..1520
                     /locus_tag="P5310_04170"
                     /inference="COORDINATES: protein motif:HMM:NF024601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA-like domain-containing protein"
                     /protein_id="HRU32587.1"
                     /translation="FAPYVHNFTPIYTWYVPTMATDGIRLFVNPKFANDLTWEQKIFV
                     IIHEIMHCVLLHMERIKGRDPELYNMAADLEINPIIIDTLNDFDEAFIRDLNGLYDKK
                     YLNQPVEAIYEDLKKNPPMAGNSFQDNKGGKEGESGKSGESGDSGQPGKGGKKGKGGG
                     VEIEVGTKVMIRSTGEKGIVTAINPDGTYEVDIINESFYPKLSNILFESYRREELVPI
                     IEGEPPADATKNSPGGVSDPAYEVIYEKMREYDPAGTGGIMTKDIGEKIAEKSGYDVE
                     SGEAGPDIDPKDKWTSESAKMLKDIERSKEYGSGKGRALLTALSRLHRGEVNWKNLFS
                     RFVSTALSPEVYQKIGNKKHLGKEYLKYGEKNKMDALENIVILVDVSGSMSMESLERI
                     LGEINSIIFSKRVKTINIAFFDDGVDEESVQRITRTSGRPWIPKNVKGGGGTDFQKAL
                     DWVKEKFRDRISLLVFFTDGYAPMPEKPIYWNKFIWVVYGNPTFKQPFGKLVNLS"
     gene            1552..3096
                     /locus_tag="P5310_04175"
     CDS             1552..3096
                     /locus_tag="P5310_04175"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRU32588.1"
                     /translation="MRKIVSESVFEFKKRKSLNEGIFRNLGNFIKKVFKRVKKFFWLV
                     IGDRAEPVILPVNIGIMVKDGILSPAISYVPNDDDLLLEPGLRTESPKSVIEKRRLIT
                     NESLNEARVPLEHPDKNVPNINRDELYMEIEMAIRDPEGKPLMIWGAPGIGKTEIVKA
                     VLEANSKGRLIDVQTSKMAPDDWNLPAIVKMEAGEVKALDIPKSWLPVYKPTGNREID
                     AKLDRIANGGTEDEDGPGGVIFLDELSRASSSVQNTCLKLIGERIIGDAKLGSKWAII
                     SASNRAGDDPDSVQNFSTALGNRFQQVNYVPDFQSWKEWGMGKIDQRILDFLEFNQEY
                     FYNLDDDPEKSIFASPRTWAAASDSIARVMQYAKDKGIRVTVPMLTRAVGKNVGLDIA
                     TEFSTFLRLLESFRKEDIEKVFNSPEEAPLPKKAGSGYDQSEANAIISLLVSSTRGRE
                     LTPEEFTNFCKYLVRLDNPSLATKGLKMMFDAHKYMHKELGEVPGHDKYKEGVDIFIE
                     KYKDIF"
     gene            3096..>3510
                     /locus_tag="P5310_04180"
     CDS             3096..>3510
                     /locus_tag="P5310_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32589.1"
                     /translation="MKKIVHENLYQFLKEWVDPYEDKWADREIDLYQKRKSGIIPEEN
                     PQDILKYPEDIPIPKDTRTAIKNIIDYFKDKDDISSEDFKNLCIWISRLYNDFDSVVE
                     KLELILSYFPYIIREIKTAKYDSKYREGLEYIINKF"
BASE COUNT         1261 a          449 c          761 g         1039 t
ORIGIN      
        1 tatttgctcc atatgttcat aattttactc ctatttatac atggtatgtt ccaacaatgg
       61 caacagatgg gataagatta tttgttaatc ctaagtttgc aaatgactta acatgggagc
      121 aaaagatatt tgttattatc catgagatta tgcactgtgt gcttcttcat atggaaagga
      181 taaaaggaag agatccagaa ttatataata tggcagcaga cttagagatt aaccctatta
      241 taattgatac tttaaatgat tttgatgaag cctttataag agatcttaat ggtctatatg
      301 ataagaaata tcttaatcag cctgtagaag ctatctatga agatttaaag aagaaccctc
      361 ctatggcggg taattctttc caagataata aaggtgggaa agaaggagag tctggaaaaa
      421 gtggagaatc tggtgatagt ggacagccag gaaaaggcgg aaaaaaagga aaaggtgggg
      481 gtgtagagat agaggttggg actaaagtta tgataaggag tactggggag aagggaatag
      541 ttactgctat aaacccagat ggaacgtatg aagttgatat tataaatgaa agtttttatc
      601 caaagctatc taatatatta tttgaaagtt atagaagaga agaattagta ccaattattg
      661 aaggagaacc tcctgctgat gctaccaaaa attcccctgg aggggttagt gatccagcat
      721 atgaagttat atacgaaaag atgagagagt atgatcctgc tggtacaggt ggtattatga
      781 ctaaggatat tggtgagaag atagcagaaa aatctggcta tgatgtagaa agtggagaag
      841 ctgggcctga tatagatcct aaagataaat ggacatctga gtcagcaaag atgcttaagg
      901 atatagagag gtctaaagaa tatggatctg gtaaaggaag agctttactt actgcactat
      961 ctagacttca tagaggggag gttaactgga agaatctatt tagtagattt gtctccacag
     1021 cactctctcc agaagtatat cagaagatag gtaataagaa acatttaggt aaagaatatc
     1081 ttaagtatgg tgaaaagaat aagatggatg ctttagaaaa tattgttatt ttagtagacg
     1141 tttccggtag tatgagtatg gaaagtttgg aaaggatatt aggagagata aacagtatta
     1201 tattttcaaa gagagtaaag actattaata tagctttctt tgatgatggg gttgatgaag
     1261 aaagtgttca aagaataact agaacttctg gtcgtccatg gattcctaaa aatgttaaag
     1321 gaggtggagg tacagatttc caaaaagcat tagactgggt aaaagaaaaa tttagagata
     1381 ggatttcact tttagtcttc tttacagatg gatatgctcc tatgcctgaa aagcctatct
     1441 attggaataa gtttatatgg gtggtttatg gtaatccaac ttttaaacag ccatttggta
     1501 aattagtaaa tttaagttaa atattttaga ataaatataa aaaaaatgtc tatgagaaaa
     1561 atagtaagtg aatctgtttt tgagttcaaa aagagaaagt ccttgaatga aggtattttt
     1621 agaaatttag gaaacttcat aaaaaaggta tttaaaagag taaaaaaatt cttttggctc
     1681 gttattggtg atagagcaga acctgttatt cttccggtga atattggtat tatggttaaa
     1741 gatggcatac ttagtcctgc tatatcatat gttccaaatg atgatgacct acttcttgaa
     1801 ccaggtctaa gaaccgaaag tcctaaaagt gtaatagaga agagaagact tattactaat
     1861 gagtcattaa atgaagctag agttcctcta gagcatcctg ataagaatgt cccaaatatt
     1921 aatagggatg agctctatat ggaaatagaa atggctataa gagacccgga aggaaaacct
     1981 cttatgatct ggggagctcc tggtatagga aagactgaga ttgttaaagc tgttctagaa
     2041 gctaattcta aaggaaggct tattgatgtc cagacttcta agatggctcc agatgactgg
     2101 aatttaccag ctattgttaa aatggaggct ggtgaggtaa aagcccttga tattcctaag
     2161 tcttggcttc ctgtttataa acctacaggt aacagagaga tagatgctaa gctagataga
     2221 atagctaatg gaggcacaga agatgaggat gggcctggtg gagttatctt ccttgatgag
     2281 ctttcaaggg cttcatcgtc tgttcagaat acatgcttaa aacttattgg tgagaggatc
     2341 attggagatg ctaagcttgg atcaaaatgg gctattatct ctgcatctaa tagagctgga
     2401 gatgaccctg actcagtaca gaacttctct actgctttag gtaatagatt ccagcaagtt
     2461 aactatgttc ctgacttcca gagctggaaa gagtggggta tgggaaagat agatcagagg
     2521 atattagatt tcttggaatt caatcaggag tatttttata atcttgatga tgatcctgaa
     2581 aagagtattt ttgcatctcc tagaacttgg gcagctgctt ctgactcaat agctagggtt
     2641 atgcaatatg ctaaagataa ggggataaga gtaacagttc ctatgctaac gagggcagta
     2701 ggtaagaacg taggacttga tattgctaca gaatttagta cattcttaag acttcttgaa
     2761 agctttagaa aagaggatat agaaaaggta tttaatagcc cagaagaagc tcctctaccg
     2821 aaaaaagctg gttcaggata tgatcagtct gaagctaatg ctattatatc tctccttgtt
     2881 agttctacaa gaggaagaga acttacccct gaagagttta caaatttctg caaatatctt
     2941 gtaagacttg ataatccttc tctagcaaca aaaggtctta aaatgatgtt tgacgcacat
     3001 aagtacatgc ataaagaatt aggagaagtc ccaggacatg ataagtacaa agaaggagta
     3061 gatattttta tagaaaaata taaggatata ttctaatgaa aaagatagta catgaaaatt
     3121 tatatcaatt tctaaaagaa tgggttgatc cttatgaaga taaatgggct gatagagaga
     3181 tcgatctata tcaaaaaaga aaaagtggga taatccccga agaaaatcct caggatattc
     3241 taaaatatcc tgaggatatt cctatcccta aggatacaag aactgctatt aagaatatca
     3301 tcgattattt taaagataaa gatgatatat catcagaaga ttttaaaaat ctttgtattt
     3361 ggatatccag attatataat gatttcgata gcgttgtaga aaaactagag cttattctat
     3421 catatttccc ctatataatt agagagataa aaacagctaa atatgatagt aaatatagag
     3481 agggtttaga atatattata aataagttta
//
LOCUS       DAOTKU010000134         3501 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_22364_length_3501_cov_3.348230, whole genome shotgun sequence.
ACCESSION   DAOTKU010000134 DAOTKU010000000
VERSION     DAOTKU010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3501
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22364_length_3501_cov_3.348230"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="P5310_04185"
     CDS             complement(<1..206)
                     /locus_tag="P5310_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HRU32590.1"
                     /translation="MTYINNFLISILDFFYNMTGNYGVALILFTVLVKLVLYPLTHQQ
                     FKALKEQQKIQPEQNKLREKYKDDP"
     gene            complement(227..448)
                     /gene="yidD"
                     /locus_tag="P5310_04190"
     CDS             complement(227..448)
                     /gene="yidD"
                     /locus_tag="P5310_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019878572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HRU32591.1"
                     /translation="MLRIGRIVALKLIGFYKIFISPLLGRNCRFYPTCSDYTYQAIEK
                     YGLIKGVYLGLKRILRCNPFNPGGYDPLI"
     gene            complement(432..755)
                     /gene="rnpA"
                     /locus_tag="P5310_04195"
     CDS             complement(432..755)
                     /gene="rnpA"
                     /locus_tag="P5310_04195"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HRU32592.1"
                     /translation="MLPREKRLSKSKDIKNILKNGKRVSMNGMSLYVLEGRGRIGFIT
                     TRGFKNAVCRNRARRRVRGAFESEKISLDNYDLLFVIRPEVSNLDFSEIQETVRGLIS
                     DVKNR"
     gene            complement(755..895)
                     /gene="rpmH"
                     /locus_tag="P5310_04200"
     CDS             complement(755..895)
                     /gene="rpmH"
                     /locus_tag="P5310_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015716485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HRU32593.1"
                     /translation="MKKTAFQRNRKPRKTTHGFLVRMSSPGGRNVIKRRRAKGRYRLA
                     PV"
     gene            complement(968..2002)
                     /locus_tag="P5310_04205"
     CDS             complement(968..2002)
                     /locus_tag="P5310_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012582635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HRU32594.1"
                     /translation="MDKEKSLRWLKVGLGLTILSSTVPFNIKRENVPDLKIASQINLT
                     GTLVSQTKEVHSIANVSIVKESSIRESVRKAISMAGDLDFIKPGDTVLVKPNVNSDDP
                     YPGTTNPEVIRELVSMCYEKGAKKVIIADRSGVSWQNTLRNMEKTGIAQAGRSVGADV
                     LALDNEEWIMVKPEKTKYWVNGFRIPKIVTEVDHLINVAVVKTHSIADFSMSLKNFVG
                     FIHMSDRMFMHSSGNLKEMIAELNLAFNPKLNILDASKVFVSGGPDRGEVKIPMMIIA
                     GDDRVAVDITGLALLRSLGTTPAIQSRDIWSHPQIKRAIELEIGIKNHSSIKFTGSNV
                     DIAQIEHLSE"
     gene            complement(2069..2500)
                     /locus_tag="P5310_04210"
     CDS             complement(2069..2500)
                     /locus_tag="P5310_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005988897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HRU32595.1"
                     /translation="MAYQKVSQELMEMLNQAIAREIQVSIQYMWQHIQWVGVKSFAVK
                     DEFRSIAIDEMKHAEIIAERLFYLGGTPTTKPSPINVGDNLKEMLEQDVKDEENAIDL
                     YKKIISAAETEGDVTTAKIFKDILEDEEEHHDTFSSLLEEV"
     gene            2719..>3501
                     /gene="aroE"
                     /locus_tag="P5310_04215"
     CDS             2719..>3501
                     /gene="aroE"
                     /locus_tag="P5310_04215"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF001319.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HRU32596.1"
                     /translation="MEINGSTRIAVVIGHPIRHSLSPVMHNAAFKYLGLNNCYVAIDI
                     SPGGIEHFIRGLRYTNIIGVNVTIPHKAAVIPYLDELSEEAKILEAVNTIKITEEKVI
                     GYNTDVYGFEKCLEDIDVPSCSALLIGAGGAGKAVAYTLSKLNVKSLYITNRTEERAK
                     QLIQFINNVFNRKIEFIPFNQLETLKPVDIIVNATPLGMEGIKEELEIPSSIIREGTT
                     AIDLVYNPPVTNFLKTASSLGAKTLNGIKMLVYQGITSFKIWT"
BASE COUNT         1099 a          735 c          537 g         1130 t
ORIGIN      
        1 ggatcatcct tatacttctc tctcagctta ttctgctccg gctggatctt ctgttgttct
       61 ttaagagcct tgaactgttg atgggtaagt ggatacagga ctaactttac aagaacagta
      121 aaaagaatca gcgcaactcc ataattacct gtcatattat agaagaagtc aagtatactg
      181 ataagaaaat tatttatata agtcataaca tctaaccttc cttaatttat attagtggat
      241 cataaccacc agggttaaat ggattacatc gaaggattcg ttttaatccc agatacacac
      301 ctttaattaa gccatacttt tctatagcct gataagtata atcagaacag gtaggataaa
      361 atctgcaatt tctccccagt aaaggggaga taaatatttt ataaaatcct attaacttta
      421 aagctactat tctacctatt cttaacatct gaaataagtc ccctaactgt ttcctgtatc
      481 tcactaaaat caaggtttga tacctcaggt cttatcacaa aaagtaaatc ataattatcc
      541 aaagagattt tttcagattc aaaggctccc cttactcttc ttctggctct attccggcag
      601 actgcattct taaatcctct ggtagtaata aagccaatcc tacctcttcc ctctaaaaca
      661 tataaactca taccattcat acttaccctt ttaccatttt ttaatatatt ttttatatcc
      721 ttagactttg ataatctctt ttccctgggg agcattacac cggagctagt ctatatcttc
      781 ccttagctct ccttctcttg ataacattcc tacctccggg ggagctcatt ctaactaaaa
      841 acccgtgggt agtcttccta ggttttctat ttcgctgaaa tgctgtcttc ttcattatct
      901 acctccttcc atccttcgaa tgaaaataat aacataattg aatgataatt acaagaagct
      961 ttaactatca ctctgatagg tgttcaatct gggctatatc cacattactg ccggtaaact
     1021 ttatactgga atggttttta ataccaatct caagctctat agccctttta atctgtgggt
     1081 gagaccatat atctctactc tgaatagctg gagtagttcc taaacttctc aatagagcca
     1141 gacctgttat atccacagca actctatcat ctccggctat tatcatcatt ggaattttta
     1201 cttcacctct atctggtccg ccacttacaa agactttact agcatcgagt atattcagtt
     1261 ttgggttaaa agcaaggttc aattcggcaa tcatttcttt taaattcccg ctactatgca
     1321 taaacattct atcagacata tgtatgaaac ctacaaagtt cttcagactc atggaaaagt
     1381 cagctatcga atgtgtttta acgacagcaa catttataag atgatccacc tctgtaacta
     1441 ttttgggaat cctaaaacca ttaacccaat acttagtttt ttcgggtttt accattatcc
     1501 actcttcatt atctaacgcc aatacatctg ccccgacact cctacctgcc tgagcgatac
     1561 cggtcttttc catatttcgc aaggtattct gccacgacac accagaacgg tctgcaatta
     1621 taaccttttt agcacccttt tcgtaacaca tggacaccaa ttcccttata acttcaggat
     1681 ttgttgttcc aggataaggg tcatcagagt ttacgttagg ttttactaac acagtatctc
     1741 caggctttat aaagtccagg tcacctgcca tagaaatagc ctttctaaca gattctctaa
     1801 tactggattc ttttacaatg cttacattag ctatcgagtg aacctctttt gtctgagaga
     1861 ccaaagtgcc tgttagatta atctgactag ctatttttaa gtccggtaca ttttcccttt
     1921 taatattaaa tggaactgta gaagaaagaa tcgtcaaacc caaccctact ttaagccatc
     1981 ttaaagattt ctctttatcc ataaaactta ccccctaata taattagggg cttacaaaat
     2041 gtaagccccc tgtaaatatt ctgaaaatct agacttcttc cagaaggcta ctaaatgtat
     2101 cgtgatgttc ctcttcatct tctaatatat ccttaaatat ctttgcagtg gttacatctc
     2161 cttctgtttc tgctgcagaa attatcttct tataaagatc tatggcgttc tcctcatcct
     2221 tgacatcctg ctccagcatc tctttaaggt tatcaccaac atttattggg ctgggtttag
     2281 tggtaggtgt cccaccaagg tagaataatc tctccgctat aatctccgca tgcttcattt
     2341 catctattgc gatacttcta aactcgtcct taacagcgaa tgatttaaca cctacccact
     2401 gtatgtgctg ccacatatac tgaatagaga cctgaatctc cctggctata gcctgattga
     2461 gcatctccat gagttcctga ctaacctttt gatatgccat aaaaacacct cctaattaat
     2521 aatttgttta tataaatttt atcattttat aagaacctat gcaagtaatc agaatgaaaa
     2581 aggcatagac gatatattat cttaccaagc tacttatcta agatagatat gatgatagag
     2641 tagcatatat ctaaagtgat tccagattat tttctatatt acttgaaaat tctctaaatt
     2701 taattataat aaaatgctat ggagattaat ggttctacaa gaatagcagt cgtgataggg
     2761 catccaatca gacatagttt atccccggtg atgcataatg cagcctttaa atatctggga
     2821 cttaataact gttacgttgc tatagatata tccccaggag gtatagaaca ttttatcagg
     2881 ggactcagat atacgaatat aattggagtt aatgttacca taccacacaa agcagcagtt
     2941 attccttacc tggatgagtt atcagaagag gcaaagattc tagaagcggt aaatacgata
     3001 aagataacag aagaaaaagt tatcggctac aatacagatg tgtatggttt tgaaaaatgt
     3061 ctggaggata tagatgtgcc cagctgttct gcccttctca taggagcagg aggagctggc
     3121 aaagcagtag catataccct cagcaaactc aatgtaaaaa gtttatatat taccaataga
     3181 acagaggaaa gggcaaaaca acttatacaa tttataaata acgtattcaa cagaaaaata
     3241 gaatttatac cattcaatca actggaaaca ttaaaacctg tagatataat agtaaatgct
     3301 actccattag gtatggaagg gataaaagaa gagttggaga ttccatcttc aataataaga
     3361 gaggggacta ctgcgataga ccttgtatat aatccacctg tcactaattt cttaaaaaca
     3421 gcctcttctc tgggcgctaa gaccttaaat gggataaaga tgctagtcta tcagggtatt
     3481 acctctttca agatatggac a
//
LOCUS       DAOTKU010000135         3488 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_22473_length_3488_cov_2.936207, whole genome shotgun sequence.
ACCESSION   DAOTKU010000135 DAOTKU010000000
VERSION     DAOTKU010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3488
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22473_length_3488_cov_2.936207"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..155)
                     /locus_tag="P5310_04220"
     CDS             complement(<1..155)
                     /locus_tag="P5310_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family peptidase"
                     /protein_id="HRU32597.1"
                     /translation="MKELIKKLTETNSPSGRESNIREVILKELDGYIDGYEVDRLGNL
                     VVWKRGKT"
     gene            complement(152..1162)
                     /locus_tag="P5310_04225"
     CDS             complement(152..1162)
                     /locus_tag="P5310_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015919983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HRU32598.1"
                     /translation="MYLKELTELSGVSGYEKRVREFIKDKIKDRVSHIEEDVLGNLIA
                     RKEGKTDKRIMLCAHMDEVGFIVSNIEDDGKLCIMPLGGIDPKVVVGKRVTVGEKEIP
                     GVIGYKAIHLQRGDVYTPPKMDEIRVDIGVTKKSDAEGKVRIGDFVAFSVESMVHGDW
                     FSGKALDNRGGCSILIDIINNNIELYYDTYFVFSVQEELGLRGAGISAEKINPDFSIV
                     VETTTSGDNPEFSSYRRSTELGKGPAITFAHSGYVIDERLFKWIIDTAKEHNIPYQIK
                     RRTAGGTDARRIATTLAGIPSAVISIPARYIHSPMSVLNLEDYNNTLGLISILLTKEV
                     QL"
     gene            complement(1165..2172)
                     /locus_tag="P5310_04230"
     CDS             complement(1165..2172)
                     /locus_tag="P5310_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRU32599.1"
                     /translation="MLTDLLERLSSIDGPSGYERDVIECMRKEAGMIPGVFTEVDILG
                     NFIVRKEGKGKKIALFAHADEIGFVLTRREEGNYWRFSTIGGIDPKAIISQRLKIYTR
                     DGDIAWGIVGMIEPHLQTDEGREKVPSLDDLFLDVVEGGERVNTGDIGVFSSKPFSLS
                     EKYFTGKALDNRASCATLLYTLELLNNFTSKSDLFFVFNIREEDGSPGAKTSAYRLKP
                     DLSIVLDVTHGDISIPSMPKIETGKGPALGVGPVIDYGYLEKLKNLADKISVSYQLEP
                     INGRSGTDTDLIQITRGGIPTLLLSLPLRYMHTPYEVVNIRDIEETARLLSYFLARGG
                     E"
     gene            2347..2583
                     /locus_tag="P5310_04235"
     CDS             2347..2583
                     /locus_tag="P5310_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32600.1"
                     /translation="MSRVVKRTISLPYDLSLEVERIAKDEGKTVSAVIQEALRILSKK
                     RLEEEFYEIQGYWSKKAKEKGIITEEDLERYLNE"
     gene            2580..2972
                     /locus_tag="P5310_04240"
     CDS             2580..2972
                     /locus_tag="P5310_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HRU32601.1"
                     /translation="MRVVFDTNIYTSALVIPGSQGEKAILKVIREEDLLIISQEIIDE
                     LIRVLVKKFSRNREAISRTILYLKSIAEVVTPVEKINIFEDEPDNRIIECALSGKADA
                     IVTGDKRMLELKEYKEIKIISLREYLSI"
     gene            3091..>3488
                     /locus_tag="P5310_04245"
     CDS             3091..>3488
                     /locus_tag="P5310_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019005569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HRU32602.1"
                     /translation="MINVTVWNEFRHEKIHEEVAKVYPDGIHNAIGNFLKKQPGLEIR
                     TATLDEPEHGLTQEVLDNTDVLIWWGHMAHNEVKDEIVDRVYNRVLNGMGLIVLHSGH
                     LSKIFRKLMGTGCNLKWREAGEKERIWVVEP"
BASE COUNT         1095 a          721 c          612 g         1060 t
ORIGIN      
        1 gtctttcccc tcttccacac aacgagatta cccaacctgt caacttcata tccatctata
       61 taaccatcca gctccttaag aataacctca cgtatattag attcccttcc acttggggaa
      121 tttgtctctg taagtttctt aataagttcc ttcataattg aacctccttg gttaaaagta
      181 tggagataag tccaagagta ttattataat cttccagatt tagaacagac attggagagt
      241 ggatatatct tgcaggtata gaaataacag cagatggaat ccctgccaaa gtagtggcaa
      301 tcctcctggc atctgttcct ccagctgtcc tcctcttaat ctgatacggt atattatgct
      361 ctttggcagt atctattatc cacttgaaaa gcctctcatc gattacatat ccactatgcg
      421 caaatgttat agcaggaccc ttccctaact ctgtagacct tctgtaggat gagaactctg
      481 gattatctcc agatgtcgta gtctctacca ctatagaaaa gtcaggattt atcttttcgg
      541 cagagatacc tgcccctctt agaccaagct cttcctggac agaaaagaca aaataagtat
      601 cataatataa ttcaatatta ttattaatta tatctatcag aatagagcag ccccctctat
      661 tatctaaagc cttaccagag aaccagtctc catgaaccat agattctact gaaaaggcta
      721 caaaatcacc tatcctgacc ttaccttccg catccgactt cttagttaca cctatatcca
      781 cccttatctc atccatcttt ggtggtgtat agacatctcc tctctggaga tgaattgcct
      841 tataacctat aaccccaggg atttcttttt ccccaactgt tactcttttc ccaacaacaa
      901 cctttggatc tattcctccc agaggcataa tacagagttt tccgtcatcc tctatattcg
      961 atactataaa accaacctca tccatatgcg cacacagcat aatcctctta tctgtctttc
     1021 cttcttttct ggctataagg ttcccaagca catcttcctc tatatgagaa actctatctt
     1081 ttatcttatc ctttataaac tctctaaccc tcttttcata accagaaacc ccactaagtt
     1141 ctgtcaactc tttcagatac aaagctattc gcctcctcta gccaggaaat aactgaggag
     1201 cctggctgtc tcttctatat ctcttatgtt aaccacctca tagggggtat gcatatatct
     1261 taaaggaaga gagagaagca gagttggtat tccacctctg gtaatctgta taaggtcagt
     1321 atctgttcca ctccttccat ttataggttc cagctgataa ctgacagata tcttatctgc
     1381 taaattcttc agcttctcaa ggtatccata atcaattaca ggtcctacac ccagagcagg
     1441 acctttcccg gtctctatct ttggcataga tggaatagaa atatctccat gagtaacatc
     1501 cagaactata gataaatcag gttttaatct atatgcagaa gtttttgcgc caggacttcc
     1561 atcctcttct cttatattaa agacaaaaaa caagtccgac ttagaggtaa aattattcaa
     1621 aagctctagc gtatagagta aagtagcgca acttgccctg ttatcaagag ctttcccggt
     1681 aaagtatttt tcagatagag aaaatggctt cgatgagaac actcctatat cccctgtatt
     1741 taccctctct cctccctcaa ccacatccag aaacaggtca tctaaagatg gaaccttctc
     1801 tctaccctca tctgtttgta aatgaggttc tatcatacca acaatccccc aggctatatc
     1861 cccatctctg gtatatatct tcagcctttg agatattata gcctttggat ctattccacc
     1921 aatagtggag aatctccaat agttaccctc ttctctcctg gtaagaacaa accctatctc
     1981 atctgcatgg gcaaaaagtg ctatcttttt cccttttccc tcctttctca cgataaaatt
     2041 tcccaggatg tcaacttcgg taaaaacccc agggatcatc ccagcttctt ttctcataca
     2101 ttctattaca tccctttcat aaccggatgg tccatcaatt gaagaaagtc tctctaaaag
     2161 atcagttaac accaatttag cctcctagac tctttcagta atgaattctt atcttcacat
     2221 aaaatttttc tctaattatc tctaattata caaatttcta tttattaaat tcaatagcag
     2281 attctatctt gattttacca gttatttatg gtattatttt ttcatactaa aataaggagg
     2341 gatggaatgt ctagggttgt aaaaaggacg atatcactcc cctatgatct atctcttgag
     2401 gttgaaagga tagcaaagga tgaaggtaaa actgtaagtg ctgttattca agaagctcta
     2461 aggatacttt ctaagaaaag gctggaagaa gagttttatg aaattcaggg atattggagt
     2521 aaaaaggcaa aagaaaaggg gattattact gaggaagatc tggaaaggta tcttaatgaa
     2581 tgagagtagt attcgataca aatatctata cttctgccct tgttattccg ggtagtcagg
     2641 gagagaaggc gatattgaaa gtcataaggg aggaagactt acttataatc tcacaggaaa
     2701 taatagatga gctgataagg gtactcgtta aaaaattcag caggaacaga gaagctataa
     2761 gtaggactat actttacctt aaaagtattg cagaggttgt aacaccagta gagaaaatta
     2821 atatttttga agatgagcct gataacagga taattgagtg tgcattatcg gggaaagcag
     2881 atgctatagt tactggagat aagagaatgc tggaacttaa agagtataaa gagataaaaa
     2941 ttataagtct gagagaatat ctgtccatat aaactagaat tttacctttt aaggtgaggg
     3001 tatttttcag ccagttcttg ctattccgtt aagagaaaat atatagtata attcaataaa
     3061 attgagaagg aaggtgataa ggtgggagag attattaatg taactgtatg gaatgaattc
     3121 cgacatgaaa agattcacga agaagtggca aaagtctatc ctgacggaat acataatgct
     3181 ataggaaatt ttctaaagaa acagcccgga ttagagataa gaactgcaac acttgacgag
     3241 cctgaacatg gtcttactca ggaggttctg gataatacag atgtactgat atggtgggga
     3301 catatggcgc acaatgaggt aaaggatgag atagtagaca gggtatataa tagggtattg
     3361 aatggaatgg ggcttattgt cctacattct ggacatctct ccaagatatt cagaaaactt
     3421 atgggtaccg gatgcaatct taagtggcga gaggctggag aaaaggaaag gatatgggtt
     3481 gttgaacc
//
LOCUS       DAOTKU010000136         3487 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_22478_length_3487_cov_2.828671, whole genome shotgun sequence.
ACCESSION   DAOTKU010000136 DAOTKU010000000
VERSION     DAOTKU010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3487
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22478_length_3487_cov_2.828671"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="P5310_04250"
     CDS             complement(<1..626)
                     /locus_tag="P5310_04250"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HRU32603.1"
                     /translation="MNYSFLDALRERILVFDGAMGTMLMRYGLTSGACSEEWNISKPE
                     RIEEIHRSYIAAGADCIETNTFGANRFRLSAHHLDDRVIELNIAGARIAKKASQGRAF
                     VVGSVGPTGKILEPFGDLSFEDAYNAFKEQVMALEEGGVDAIIIETMGDLQEARVALL
                     ACKENTELPVICQMSFSENLRTTSGTPPEVSALVLWSLGADVVGANCS"
     gene            complement(607..969)
                     /gene="rsfS"
                     /locus_tag="P5310_04255"
     CDS             complement(607..969)
                     /gene="rsfS"
                     /locus_tag="P5310_04255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HRU32604.1"
                     /translation="MKSTLDLIYTAISAAVEKKGENIVLLDLQELFTITDYFLIITSQ
                     TSHHTSAIVESIDEVMSKEGLPPIRIEGEYEGGWVILDYKSFVIHIMEPDVREFYNLE
                     RLWARAKKVNLPDELLVS"
     gene            complement(953..1555)
                     /gene="yqeK"
                     /locus_tag="P5310_04260"
     CDS             complement(953..1555)
                     /gene="yqeK"
                     /locus_tag="P5310_04260"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR00488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HRU32605.1"
                     /translation="MDYIENRDFYISFLRENLTGERFLHTLAVEREAILLAERYNVDP
                     GKASLAGLLHDCAKCFSDEELIRLARSYNIPLSIWDIYSPQILHAPVGSIFAREHFNI
                     KDGDVLGAIAKHTTGDREMSTLDKIIFIADYVEYHRDFPGVDYLREILKDDLDNAVSF
                     AINLTIEYIMRNKSVVHPRTIETWNSLIKEGKKLIDEIYS"
     gene            complement(1536..2135)
                     /gene="nadD"
                     /locus_tag="P5310_04265"
     CDS             complement(1536..2135)
                     /gene="nadD"
                     /locus_tag="P5310_04265"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015327544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HRU32606.1"
                     /translation="MNSDKIGLMGGTFNPIHYGHLVAAEEARHQFNLDQVIFIPSGQP
                     PHRTPRSVIPAEDRYIMTALAIATNPYFNISRVEIERPGPSYTIDTVRAFKEIYPKSE
                     LFFITGLDAILEILTWRDPYELVKLTHFIAVTRPGYNPDCLKYLDKKILDGVSLLEIP
                     GYAISSTLIRERVAQGRPVRYMVPEPVEAYIRKHGLYRE"
     gene            complement(2125..3300)
                     /gene="obgE"
                     /locus_tag="P5310_04270"
     CDS             complement(2125..3300)
                     /gene="obgE"
                     /locus_tag="P5310_04270"
                     /inference="COORDINATES: protein motif:HMM:TIGR02729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HRU32607.1"
                     /translation="MFVDEVNIIVSGGDGGPGALSFHREKYLPKGGPDGGDGGKGGDV
                     YITGDRDLETLNFYLSKKRLSAGSGSQGQGNRKSGKDGEDLYLRVPLGTCVYNSENGR
                     FLGEVLFQDDKILVAKGGRGGRGNSNFLSNKYRAPRVVERGETGEKAKITLVLKSIVD
                     VAILGFPNSGKSTLLSLITNAHPKVGDYPFTTTEPVFGVLSDKVRPVKFVEIPAIVDG
                     SHKGLGLGNKFLRHAERASVLLIMFNEEKEIEIVRNELSYYSEDLLKKSTVTFPKNDL
                     FIENKIEELKEEILKLLDYSKEVFSSKEDKQSDVELQPLYIEKIENTFYVSSNDLIRA
                     IRRIDFRQRDYHLLLNPLIRKFGLLDALKNSGAKDGDKVIIGGIPFTMLEGRIRCEL"
     gene            complement(3302..>3487)
                     /locus_tag="P5310_04275"
     CDS             complement(3302..>3487)
                     /locus_tag="P5310_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015542711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HRU32608.1"
                     /translation="RSDGQFVVAGNIIVRQRGTRIHPGQNVGMGKDFTLFALTDGIVS
                     FRTKKDKKMVSVIPVQS"
BASE COUNT          980 a          871 c          602 g         1034 t
ORIGIN      
        1 atactgcaat tcgccccgac gacatctgcc cccagactcc agagaaccag agcggatacc
       61 tctggtgggg tcccagatgt tgtacggagg ttctccgaga aagacatctg acagataaca
      121 ggtaattcgg tattctcttt acaggcaaga agtgctaccc tggcttcctg aaggtctccc
      181 atagtttcaa tgataatggc atctactcca ccttcttcta atgccatcac ctgttcctta
      241 aatgcattat aggcatcttc aaaagacaaa tctccaaatg gttccaggat tttacccgtc
      301 ggtcctaccg aaccaaccac gaaagctctc ccctgagatg cctttttagc tatccttgcg
      361 ccagctatat ttaattctat aaccctgtca tcaagatgat gtgcagagag tctgaacctg
      421 tttgcaccaa aggtattggt ctctatacaa tcagcccctg cagctatata tgacctgtgt
      481 atctcctcaa ttctttcagg tttagaaata ttccattcct cggagcatgc accagacgta
      541 agaccatacc tcataagcat ggtgcccatg gcaccatcaa ataccagtat cctctctctt
      601 aatgcatcaa gaaacgagta attcatctgg aagattcacc ttctttgccc ttgcccatag
      661 tctctccaga ttatagaact ctcgcacatc tggttccatt atatgtatca cgaaagactt
      721 ataatccagg ataacccagc ctccttcata ctccccctct atacgaatgg gagggagtcc
      781 ctctttagac ataacctcat ctatgctctc cacaatagcg gatgtatgat gtgatgtttg
      841 agatgtgatt attaaaaaat aatctgtaat agtaaagagc tcctgaaggt caagcagaac
      901 aatattttcc cccttctttt ctaccgctgc gcttatggcg gtatagatta aatcaagagt
      961 agatttcatc tataagtttc tttccctcct ttattaaaga attccaggtt tcaatcgtcc
     1021 ttggatgtac tactgactta ttcctcataa tatactctat ggtaagatta atcgcaaacg
     1081 aaacagcatt atccagatca tctttcaata tctcacgcag atagtcaaca ccgggaaaat
     1141 ctctgtgata ttcgacataa tcggcaataa aaataatctt atccagagtg gacatctccc
     1201 tatcaccagt ggtatgtttt gctatagcac caagaacatc cccatccttt atattaaaat
     1261 gttctctggc aaagatactc cccacggggg catgaagaat ctgaggagag tagatgtccc
     1321 agatggacag aggtatgtta taagaccttg ccagccttat aagttcttca tcagagaaac
     1381 attttgcaca atcgtgtaaa agacctgcca gagatgcctt cccggggtca acattatatc
     1441 tttcagcaag caagatggct tctctctcta cagccagagt atgcaagaat ctttcgccag
     1501 taagattttc cctcaagaag gagatataaa agtctctatt ctctatataa tccatgcttc
     1561 cttatataag cctctacagg ttcaggaacc atatacctta caggtctacc ctgagctacc
     1621 ctttccctta tcagagtgga ggatatcgca taaccaggta tctcaagtag agaaacgcca
     1681 tcaagaatct ttttatcaag atactttaag caatcaggat tataacctgg acgggtcaca
     1741 gctataaagt gggtcagctt tacaagttcg tatggatctc tccaggtaag aatctcaagt
     1801 atcgcatcaa gccctgtgat gaaaaatagc tctgactttg gatatatctc tttgaaagcc
     1861 ctcacagtgt ctatcgtgta agacggacct ggacgctcta tctctaccct ggaaatatta
     1921 aagtaaggat tggttgctat agccagagct gtcattatat acctatcctc agccgggata
     1981 acagaccggg gggttctatg gggtggctgt cctgaaggta taaatatcac ctgatcgagg
     2041 ttaaattgat gcctggcttc ctctgccgct acaagatgtc cataatgtat gggattaaat
     2101 gtccctccca ttagtccaat cttgtcagag ttcacacctg atccttcctt cgagcatcgt
     2161 aaatggaatc cctcctataa taaccttatc accgtctttt gccccactat tttttaaggc
     2221 atctaacaga ccaaattttc ttatgagggg attcagaagg aggtgatagt ccctctgtct
     2281 aaaatctatt cttcttatag ctcttatgag atcattgctg gatacataaa aggtattctc
     2341 tatcttctct atgtatagtg gctgcaattc tacatccgat tgcttatcct ctttagagga
     2401 aaaaacctct ttagaatagt caagtaactt taaaatttct tccttcaatt cttctatttt
     2461 attttcgata aacaggtcat tcttaggaaa agtcaccgta gactttttca gaaggtcttc
     2521 actgtaatat gacaattcat ttcttactat ttctatctct ttctcttcat tgaacatgat
     2581 aagcaaaaca gaagccctct cggcatgtct cagaaatttg tttccgagtc ctaaaccctt
     2641 atgagaacca tctacaattg ccggaatctc aacaaacttt acaggtctca ccttatcact
     2701 taaaactcca aatacaggct cggtggtagt aaatggataa tctccaactt tcggatgggc
     2761 atttgtgatc agagagagca gagtcgactt acctgagttt ggaaatccca ggatagcaac
     2821 atccactata gacttcagca ccagggtgat cttagccttc tccccagtct ctcctctctc
     2881 aactacccga ggggctctat acttattgga caggaagtta ctgtttcccc ttccccctct
     2941 gccacccttt gctactagaa tcttatcatc ctgaaagagg acttcgccta aaaatcgacc
     3001 attttcagag ttatagacac aggtaccaag aggaactcta aggtacaggt cttccccatc
     3061 ttttccactc tttctgtttc cctgcccctg agagccagac cctgctgaca acctcttttt
     3121 ggagaggtaa aaattaaggg tctccagatc cctgtcccct gttatataga catctccccc
     3181 cttacctcca tctccaccat caggaccacc tttcggaaga tacttttccc tgtgaaaact
     3241 taaagcacca ggtcctccat cccctccact gactatgata tttacttcat ctacaaacat
     3301 actaagactg aacaggtatc acactcacca tcttcttatc tttttttgtt ctgaaggaga
     3361 caataccatc cgtcagggca aagagggtaa aatctttacc catcccaaca ttctgtccgg
     3421 gatggatcct ggttcctctc tgtcttacaa taatatttcc tgccactaca aattgaccat
     3481 cagaacg
//
LOCUS       DAOTKU010000137         3486 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_22482_length_3486_cov_4.325270, whole genome shotgun sequence.
ACCESSION   DAOTKU010000137 DAOTKU010000000
VERSION     DAOTKU010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3486
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22482_length_3486_cov_4.325270"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..979)
                     /locus_tag="P5310_04280"
     CDS             complement(<1..979)
                     /locus_tag="P5310_04280"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011099248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HRU32609.1"
                     /translation="MEGNKGYIIRVSGPLVVARGIPRARMFDVVRVGELGLIGEIIEL
                     RREDIYIQVYENTSGLGPGEPVETTGFPLSVELGPGLIGAIFDGIQRPLRSVAERVGE
                     YITRGIDVPSLDRDKRWHFKPLVKRGARVESGDIIGEVKETETITHRIMIPPGIRGTV
                     EEIKEGDFTINEVIAEVRDGEKNYSVTMLQRWPVRLSRPIKEKLPPREVLVTGQRVID
                     TFFPVAKGGTACIPGPFGSGKTVVQHQLAKWADADIVVYVGCGERGNEMTDVLLEFPH
                     LTDPRTNRPLMERTVLIANTSNMPVSAREASVYTGITIAEYFRDMGYNVA"
     gene            complement(969..1280)
                     /locus_tag="P5310_04285"
     CDS             complement(969..1280)
                     /locus_tag="P5310_04285"
                     /inference="COORDINATES: protein motif:HMM:NF014092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HRU32610.1"
                     /translation="MAYRVAVVGRKEFLSIYEVLGIDIFEARTKEEVIRKVKDLKDYG
                     LVLIDEEFADVLSVEREERLPILGVIPTQGGSDIKDFFSNFVIRAVGTKIFSEGNNNG
                     R"
     gene            complement(1270..2235)
                     /locus_tag="P5310_04290"
     CDS             complement(1270..2235)
                     /locus_tag="P5310_04290"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HRU32611.1"
                     /translation="MQVTFKENLDFASVVGKIRALSARLFTAGDWERLLTAKSGSDLM
                     RLIAETHYGRVVSGEDDFDKLSSKLVHNFLSEINEIEKMCPENYFLYYFKYKIDLGNI
                     KKVLYGILSKKDVTLYDGGLIPLEVLERFINNGEIELVKTLPYPWDILKYIDEEPVLW
                     TYYLEQAYLGGLIELSREYDYNLLESLIATQIDLENFKIALRIKTVNYPEKYREFFYK
                     GGSIDIEKYIELLSIPLKEWKGSDLIERLGLVESLDDPILLEKVIDEKLINLLSISKY
                     TAFGYEPVLDYFFRKEIEHRNILFLFSALEAHLPADILRSGIRGL"
     gene            complement(2226..2816)
                     /locus_tag="P5310_04295"
     CDS             complement(2226..2816)
                     /locus_tag="P5310_04295"
                     /inference="COORDINATES: protein motif:HMM:NF014093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033178 - proton-transporting two-sector
                     ATPase complex, catalytic domain [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E family protein"
                     /protein_id="HRU32612.1"
                     /translation="MGFNELRDRIISEAEKTSREIIKEANIEANNILEEALLEAENIK
                     SSGLRSIERDINMLRSQSLASIKLEMQKNILGEVQSILEELVSKASEEIINSPEYFSY
                     LLEGISDLELKGDEEILLSRYDIQRFGDRLINEIRKLKGAINLTISEGEIKGGFILKG
                     REFDINNSLEISLQNIRPEIEQMVWQIFREAEAECR"
     gene            complement(2829..3299)
                     /locus_tag="P5310_04300"
     CDS             complement(2829..3299)
                     /locus_tag="P5310_04300"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HRU32613.1"
                     /translation="MLGTALAILGSAIAVFGGGIGSSVGVGIVGQAAAGLLSEQPEKF
                     GNLLLLAAIPGTQGIYGFLAGFLVISKLGLLGGTPVTPDLYQGFQILFACLPVAVSGL
                     VSGIHQGKVSAAGVSMVAKRAEEAGRALILAVLVETYAILGLLATILLLNGIKL"
     gene            complement(3300..>3486)
                     /locus_tag="P5310_04305"
     CDS             complement(3300..>3486)
                     /locus_tag="P5310_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009247448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32614.1"
                     /translation="GVVIFIGGHIFNLVINAFGAFVHSSRLQFVEFFGKFFEGGGREF
                     NPLKKTGTYTIEKEVTE"
BASE COUNT         1001 a          891 c          481 g         1113 t
ORIGIN      
        1 gagcgacatt atatcccata tctctaaaat attctgctat agttattcct gtatatactg
       61 aagcctctct ggcagagact ggcatattgg atgtgtttgc tataagaacc gttctttcca
      121 ttaagggacg attcgttctg gggtctgtaa ggtggggaaa ttctaggaga acatctgtca
      181 tctcgttacc acgttctcca catcctacat acaccactat atcggcatca gcccatttag
      241 ccagctgatg ttgaactacc gtcttaccgc ttccaaatgg acccgggata caggcagttc
      301 caccctttgc taccgggaag aacgtatcta taaccctctg acctgtcacc agaacctctc
      361 tgggtggaag tttttccttt ataggtctgg aaagtcttac cggccatctc tgtaacattg
      421 taacactgta attcttctct ccatccctta cctcagctat aacttcattt atagtaaaat
      481 ctccctcttt tatctcttct acggtccctc tgatccctgg aggaatcatt atcctgtgtg
      541 ttatggtttc agtctctttc acctcaccta taatatctcc actctctact ctggctcccc
      601 ttttaaccag gggtttaaaa tgccatcgtt tatctctatc cagagacggc acatctatcc
      661 ctctggtgat atactcccct accctttctg ccactgatct taaaggtctc tgtataccat
      721 caaaaatagc ccctataagt ccaggaccta actccacact gagaggaaac ccggtagttt
      781 ccactggttc tccaggtcct aaacctgaag tattctcata gacctgaata taaatatcct
      841 ctcttctaag ctctataatc tctccgataa gccctaattc tccaaccctt acaacatcaa
      901 acattctcgc cctgggaata cctctggcta ctaccagtgg accagacact cttataatat
      961 atcccttgtt accttccatt attatttccc tccgaaaaaa tcttagtacc taccgctctt
     1021 atcacaaagt tggagaagaa atcttttata tcacttccac cctgcgtagg aatcacacca
     1081 agaatgggaa gtctttcttc tctctccaca gataacacat cggcaaactc ttcgtctata
     1141 agtaccagac cataatcttt taaatctttc acttttctga taacttcctc ttttgtcctt
     1201 gcttcaaata tgtcaatccc caagacttca tatatggata gaaattcctt tctaccaact
     1261 acagctactc tataagccac gtatcccact ccttaatata tcggctggaa gatgagcctc
     1321 caatgctgaa aataagaaaa ggatatttct gtgttcaatc tcttttctaa agaaataatc
     1381 aagtacaggt tcgtatccga acgcagtata tttacttata cttaaaagat ttataagttt
     1441 ttcatctata accttttcca gtaatatagg gtcatccaga gattctacta acccaagcct
     1501 ttctataaga tcggacccct tccattcctt aagaggaata ctcaataatt caatatattt
     1561 ctcgatatct atacttccac ctttatagaa gaactcccta tacttttcag gatagtttac
     1621 cgtctttatc ctcagagcta ttttaaaatt ctccaggtct atctgggtag cgataagaga
     1681 ctctaataaa ttataatcat actccctgct taactctata agacccccaa gatatgcctg
     1741 ctccagataa tatgtccata aaacaggctc ctcatctata tacttcagta tatcccaggg
     1801 atatggtaag gtctttacca gttcaatctc cccgttattt ataaatctct ccaatacctc
     1861 aagggggatt aaacctccat cgtatagagt aacatctttt ttgctcagaa tcccatataa
     1921 gaccttcttt atattcccca ggtctatctt atacttaaag taatagagaa aataattctc
     1981 aggacacatc ttctctattt cgtttatctc tgaaagaaaa ttatgtacca gtttggatga
     2041 taacttatca aaatcatctt caccagatac aaccctacca tagtgagttt cagcaattaa
     2101 cctcattaag tctgaaccac tctttgcagt cagtaatctc tcccaatcac cagctgtaaa
     2161 taatctggca gaaagagctc ttattttacc tactacagag gcaaaatcca gattctcctt
     2221 aaatgttacc tgcattctgc ctctgcctct ctgaaaatct gccagaccat ctgttctatc
     2281 tctggtctta tattctgcag ggagatctct aaagagttat ttatatcaaa ttctctacct
     2341 ttcaatataa atccgccttt tatttcaccc tcggaaatag tcagattaat ggctccttta
     2401 agtttcctta tctcatttat taaacgatct ccaaatctct gaatatcata acgtgaaagt
     2461 aatatctcct cgtcaccctt taattctaaa tcagatatac cttctaaaag ataggaaaag
     2521 tattccggac tatttataat ctcctctgaa gctttagata caagttcttc cagtatactc
     2581 tgaacctctc caagtatatt tttctgcatc tccaatttga tactggctaa actctgactc
     2641 cttaacatat taatatctct ctctatactt ctcaatccag aagattttat gttctccgcc
     2701 tctaatagag cttcctccag tatattatta gcctctatat tcgcctcttt tattatttct
     2761 ctcgaagtct tctctgcttc agagattata cggtccctaa gttcattaaa acccattgtc
     2821 ctatctcctt ataactttat accgttaagt agaaggatag tagctaaaag tcctaatata
     2881 gcgtaagttt caacaagaac agccagaatc aatgcccttc ctgcttcctc tgctctttta
     2941 gccaccatac ttactccagc agccgaaacc ttaccctgat ggataccgga gacaagtcca
     3001 cttacagcaa ctggaagaca ggcaaacaaa atctgaaaac cctgatataa atctggtgtt
     3061 accggtgtgc ctcccaaaag accaagcttg gagatcacta agaatccagc taaaaaaccg
     3121 tatataccct gtgtacctgg aatagcggca agtaggagta ggttcccaaa cttttctggt
     3181 tgttcactta aaagtccagc agctgcctgt cctactattc ctaccccaac actggaccct
     3241 atgccaccac cgaaaactgc tatcgcagat ccaagaattg ccaaagctgt tcctaacatc
     3301 tattctgtta cctccttttc tattgtgtat gttcccgtct tttttagagg attaaattct
     3361 cttcctccac cctcaaaaaa ttttccaaag aattctacaa actgtaatct gctcgagtgt
     3421 acaaaagctc caaaagcatt tattaccaga ttgaagatat gtccacctat aaagattacc
     3481 acccca
//
LOCUS       DAOTKU010000138         3470 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_22602_length_3470_cov_4.065007, whole genome shotgun sequence.
ACCESSION   DAOTKU010000138 DAOTKU010000000
VERSION     DAOTKU010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3470
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22602_length_3470_cov_4.065007"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="P5310_04310"
     CDS             <1..186
                     /locus_tag="P5310_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32615.1"
                     /translation="RLIRDNIQVVRKKKVEVGGISEGEAEITSGLNQFERVVTYSTRP
                     LNDYDRVIVIEREKWSE"
     gene            174..1232
                     /locus_tag="P5310_04315"
     CDS             174..1232
                     /locus_tag="P5310_04315"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRU32616.1"
                     /translation="MVRIAQGSRIVVSIMLISIILGSLESIRFALGRDIFTYGMDIFA
                     VSPYLTEEMNLEQLTWEDARAIADIPGVRSVDSIKISRAEVLIYDTQTNMNITYTTPD
                     FIDISGRKITDGRNFRSTDFSKSYRVVILPENTRDLLYSKDVNPLGSRLYINGEDFTV
                     IGTLSQSFIEKIVNRFKVLRDYLPMISQLVDLIYPKRELIVPLYYNPYDKLDAIIITV
                     DAKHSRGTMKAVLKDEIVKTLRFRHLQESDYEVFNVHERLLVLKVYLGLILGILFLVS
                     ILLIISGLKKTRSMDYRDKVLFLLTSVSIGELLGIILSRVITLEIDFPPAPLWVFFIG
                     LIIPLYTCGVYEIKKLKL"
     gene            1207..2103
                     /locus_tag="P5310_04320"
     CDS             1207..2103
                     /locus_tag="P5310_04320"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRU32617.1"
                     /translation="MKLKSLNYKKKNILMSYIAISPWLIGFAVFFAFPLLFSIVLSFL
                     KWSFNGSTFIWLDNYKEALFNDGDFWYSLGVTLGYGAISIPFTILFSFLLSLLINQKV
                     KGIGFFRMVYFLPVVAASDVVVAKIGPEIFSRMVNVNVTLESFGIQLSQNMQNFISLF
                     STMLTLGLWRVGIQMLIFLAGLQSIDPQVYEAAEIDGASWWKSLWSITIPLLGPFTII
                     NIVFTVIESFTNVFTIARVLAQGRSVTPFFMDYVYRLSFEEYRLGYGAAVSWIFLIIV
                     ISVVLLVYYYSQRYLIPTQRGE"
     gene            2103..2996
                     /locus_tag="P5310_04325"
     CDS             2103..2996
                     /locus_tag="P5310_04325"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRU32618.1"
                     /translation="MQLKSSSFLYKVRKVIPKIILYLLLIDGAFIFLYPLMRSAITSV
                     MTVEDYLDPSVNWIPNKLNFKNYIDVLEPMGFSNALSNTVKMTLFCVLGQTLSSALVG
                     YGLSRGKIPGKEIIMMLILATLVIPPQTLMIPYYILYHKLNLLDSLNVFIIPSFFSSG
                     IYAPLCIFLFRQVFNTLPTELEDAAIIDGADFFRCFWYIYLPLSRTAVITVGLFTFVW
                     YWNEYARGIIFFNNIRTLPIALSLLFSTGPEFMGFGYTNVVTYPMMMAAVMLTMLPCL
                     ILYLFTQRYYLTAIEQVGIKG"
     gene            3202..>3470
                     /locus_tag="P5310_04330"
     CDS             3202..>3470
                     /locus_tag="P5310_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008826793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRU32619.1"
                     /translation="MMNAILGLFHREYMQIKALDNICFEIEEGELVGYIGPNGAGKST
                     TIKILSGILVPDAGYCSVSGRIPWENRIVHVKNIGVVFGQRTQLW"
BASE COUNT         1019 a          637 c          721 g         1093 t
ORIGIN      
        1 aggctgataa gagataacat tcaagtggtg aggaagaaaa aggttgaagt tggaggtatc
       61 agtgagggtg aggcggagat taccagtgga ctgaaccagt tcgaaagggt tgttacttat
      121 agcaccagac ccctcaacga ttacgacagg gttatagtga tagagagaga aaagtggtca
      181 gaatagctca gggctccaga atagtggttt ctattatgct tatctcgatt attctcgggt
      241 ctttagaatc tataagattc gctcttggaa gggatatttt tacctatgga atggatatat
      301 tcgcagtttc tccctatctt acagaggaga tgaatctaga acagcttacc tgggaggatg
      361 ccagagccat agcagatata cctggggtga gatcagttga cagcatcaag atatctaggg
      421 ctgaggtttt gatatacgat actcagacca atatgaatat aacatatact acaccagatt
      481 tcattgatat ctctggtcgt aagataactg atggcagaaa ctttagaagt accgacttct
      541 ctaaatccta cagagtggtc attttgccag aaaataccag agatttatta tacagtaaag
      601 acgttaatcc cctggggagc agattatata taaatggaga agattttacc gttattggca
      661 cactctccca atcatttata gaaaagatag ttaacaggtt taaagtattg agagattatc
      721 tacctatgat atctcaactg gttgacctga tataccccaa aagggaactg atagttccac
      781 tctactataa cccttatgat aaactggatg ctataataat aacagttgat gctaaacata
      841 gcaggggaac tatgaaagct gttttaaagg atgaaatagt gaagacctta cggttcagac
      901 atcttcagga gagcgattat gaggtgttta atgtccacga gagattactt gtactaaagg
      961 tatatcttgg tttgatcctg ggaatcttat ttttagtatc aattctactt ataatctccg
     1021 gcttgaaaaa gaccagatct atggattaca gagacaaggt cctcttctta ttgacatctg
     1081 tctctattgg agagcttctc ggtattatac tatccagggt gatcacactt gagattgatt
     1141 ttccccctgc cccactgtgg gtcttcttta tcggtttgat tatacctcta tatacgtgtg
     1201 gtgtatatga aattaaaaag cttaaattat aaaaagaaaa atatactgat gagctatata
     1261 gccatatctc cctggctgat cggttttgca gtcttctttg ccttccccct tttattctca
     1321 atcgtcttga gctttctaaa gtggagcttt aatgggagta cgtttatatg gctggacaat
     1381 tacaaagagg cattattcaa tgatggtgat ttctggtaca gtctgggggt tactctgggt
     1441 tacggggcga tcagcatacc attcaccatc ctattctcat ttctcttatc ccttcttatc
     1501 aatcagaagg taaagggtat aggatttttc aggatggtat attttcttcc tgttgtagct
     1561 gcttcagatg tagttgtggc taaaatagga ccagagatat tcagcaggat ggttaatgtt
     1621 aatgtaaccc tggagagttt cgggatacag ttaagtcaaa atatgcaaaa ctttatctct
     1681 ctcttttcca ccatgcttac acttggtcta tggagggttg gcatacagat gcttatattc
     1741 ctggctggac ttcagagcat cgatcctcaa gtgtacgaag cagcggagat agatggagct
     1801 agctggtgga agagcctatg gtccataacc ataccacttc tgggtccgtt taccatcatt
     1861 aatatagtct ttactgttat agaatctttt acaaatgtat ttacaatagc aagagtcctt
     1921 gcccagggga gaagtgtcac tcctttcttt atggattatg tctatcgatt aagttttgaa
     1981 gagtacagac ttgggtatgg ggctgcggta agctggatat ttttaattat agttatttcc
     2041 gttgttttat tggtgtacta ttattctcag agatatctta tccctacgca gagaggtgaa
     2101 tagtgcagct taaatcttct agtttcctct ataaagttag aaaggttatt ccaaaaatta
     2161 ttctctatct tctcctcata gatggggcat ttatatttct ctatcccctt atgagaagtg
     2221 ctattacatc tgttatgacc gtagaagatt atctggaccc atctgtaaac tggattccca
     2281 ataagttgaa ctttaaaaat tatatagatg tcctggaacc tatgggcttc tctaacgccc
     2341 tatcaaacac tgtaaagatg accctcttct gtgtcctagg gcagacactc tcttcagccc
     2401 tggttggtta tggactttct agagggaaga tccccggcaa ggagatcatt atgatgttaa
     2461 tattagccac cctggttata cctccccaga cgttgatgat tccgtattat atcctgtatc
     2521 ataagctgaa cctgctggac tctctcaatg tatttataat tccatcattc ttctcttctg
     2581 gcatatatgc ccctctctgt atattcctct tcagacaggt attcaataca ttgccaactg
     2641 agcttgaaga tgcagcgata atagacggtg ccgatttctt cagatgcttc tggtacattt
     2701 atctgcctct atccaggaca gcagttatta ctgttggact ttttaccttt gtctggtact
     2761 ggaacgaata cgccagaggt atcatcttct ttaataacat cagaaccctt cccatagctt
     2821 tatcattact gttctctacc ggtccagagt ttatggggtt tggttacaca aatgtggtta
     2881 catatccgat gatgatggct gcagttatgc ttaccatgct accctgcttg attctctacc
     2941 tatttactca aagatattac ttaacagcta tagagcaggt gggtataaaa ggatagccca
     3001 tctataattg gtacagagat aaatctgtag aaaacagagc attatcctgt acttataagg
     3061 tgtccccgcg atagagaaaa taaagaataa tggtataatt atcatatact gatgcgaaaa
     3121 taatccaaag gagaagattt gtctcaaata tcggtaaaaa acataaataa aaccttcacg
     3181 atttcaaaaa gagaaaaggg aatgatgaat gccattctgg gtcttttcca tagggaatat
     3241 atgcaaataa aagcactaga taatatctgt tttgaaatcg aagagggaga actggtggga
     3301 tatattggac ctaatggggc aggaaaatca accacgatta agatattaag tgggatactc
     3361 gtccctgatg ctggatactg ttctgtttca ggaagaatcc cctgggagaa tagaatagtt
     3421 cacgtaaaaa atataggggt cgtctttggt cagagaactc aactctggtg
//
LOCUS       DAOTKU010000139         3437 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_22840_length_3437_cov_3.593436, whole genome shotgun sequence.
ACCESSION   DAOTKU010000139 DAOTKU010000000
VERSION     DAOTKU010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3437
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22840_length_3437_cov_3.593436"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            42..1421
                     /locus_tag="P5310_04335"
     CDS             42..1421
                     /locus_tag="P5310_04335"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HRU32620.1"
                     /translation="MLDERVDAIGIRTVHLERTETAEDGIFRFIINGIPVRARGSNWV
                     PLDALHSRDKERTIPVLELFDDLGCNIIRCWGGGVYESEEFFNFCDSHGIMVWQDFAL
                     ACARYPQREEFQKVLEEETRKVIRRLRNHPSLILWAGDNECDYAYNLENLLPSDNILT
                     RKVLPSVCKQLDPYRPYLPSSPYLSDIVVRSKGKLSPPEQHLWGPRDYYKSRFYTENN
                     AEFISEIGYHGCPNLESLKRFIPEEYLSTWDNDYWRAHSVEHWRTRRRSYNRNELMAN
                     QIKELFGFIPEKIEDFILASQISQAEAKKFFIERARFSDNIWGIIWWNVMDCWPQISD
                     AIVDYYFSKKLAYSYIKRVQEPFFIMISEPEGWYVKVVGINDTLSRKEGTFIISDADG
                     GEKVIDGNYSIEPLSKKVLGMIRVSRGEQKLFIINWQLDGKSYKNHYLLGNPPFSLER
                     YTDWMGKIL"
     gene            1437..>3437
                     /locus_tag="P5310_04340"
     CDS             1437..>3437
                     /locus_tag="P5310_04340"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HRU32621.1"
                     /translation="MSKKFNLRMIGNAHIDPVWLWKWEEGLETTRATFKSAIDRLEEY
                     PEFVFTASSACLYRWIEETDPELFEKIREKVKEGRWCITGGWWIEPDCNIPSGEALAR
                     QGLYGQKYFEEKFGVKAKVGYNPDSFGHNWMIPQILKKMGIDYYVFMRPGPHEKELPG
                     DIFYWESPDGSKVLTYRIPFSYATWGKDVEKQVRKVAEKLDENISSLMCFYGVGDHGG
                     GPTRENIENIIELNRREDIPELIFSSPDRYFERLISKKREFPVVKDDLQHHASGCYSV
                     LSWIKKTNRKLEHLLLNSEKIAVLGNLIKNRPYPRKEITSGWKTLLFTQFHDILAGTS
                     IPEAYEEIKAMHGAVEDNTTSIVIYTTQSIAKEIDTSGEGEPIIVFNTTSFERNVPVE
                     IELPWREENLSILDENKLPVISQLSRPSATTAGGRVKVSFIADLPPLGYKVYRAVPAD
                     ISIPYKHSLNIKENILENDWLYLEIESDTGYIKRLYDKENKRDIFRGEAAIPVVINDT
                     SDTWSHNVFKFKEEIGSFSNAKIKITENGPDKGTIRVENFYNSSTVIQEFTLYRELKS
                     VEVKVTVNWNEKLKMLKLRFPINIEEPNATFSIPYGFISRPCNGEEEPGGEWVDAGNG
                     NYGLSLINDAKYSYDMDGNVLSLTVLRSPVYAHHIPRELDPNE"
BASE COUNT         1159 a          567 c          832 g          879 t
ORIGIN      
        1 gacctttaca gagtgaagtg tgagctttta tacaatggga agtgctggac gagagagtag
       61 atgcgatagg catcagaaca gttcatctgg agaggacaga gacagcagag gatggaatat
      121 tcagatttat tataaatggt atcccggtga gggcgagagg ttccaactgg gttcctctgg
      181 atgccctcca ttcaagagat aaggaaagaa ctatacctgt tcttgagtta ttcgatgacc
      241 tgggatgtaa tattatcagg tgctggggtg gaggggttta tgagtctgaa gaattcttca
      301 acttctgtga tagccatgga ataatggtct ggcaggattt tgcccttgcc tgtgcaaggt
      361 accctcaaag agaagagttt caaaaagtgc tggaggagga aactagaaaa gttataagga
      421 gattgagaaa ccatccaagc ttaatcctat gggcaggaga taacgaatgt gactatgcat
      481 ataatctgga gaatctctta ccatctgata atatacttac caggaaggtg ttaccatctg
      541 tatgtaagca gttagatcca tatagaccct atctaccttc ttctccttat ctatcagata
      601 tagttgtaag atcaaagggg aaactctctc cacctgaaca gcatctctgg ggaccaaggg
      661 attactataa gagtagattc tatacagaaa ataatgcaga atttataagt gagataggtt
      721 atcatggatg tccaaatctg gagagtctga agagatttat ccctgaagaa tatctatcaa
      781 cctgggacaa tgattactgg agagctcact ctgtagagca ctggaggaca agaaggagaa
      841 gctataacag gaatgaactg atggctaatc agataaagga gctatttgga tttattccag
      901 aaaagataga ggattttatc ctggcaagtc agatctctca ggctgaggca aagaagtttt
      961 tcatagaaag ggcaaggttt tcagataata tctggggtat tatctggtgg aatgtgatgg
     1021 actgctggcc tcagatctcc gatgcaatag tggattatta tttcagtaaa aaacttgctt
     1081 attcttatat aaagcgggtc caagagccat tctttataat gataagcgag ccagaaggct
     1141 ggtatgtaaa ggtggtagga ataaatgata cactctcaag aaaagaggga acatttatca
     1201 tatcggatgc agatggaggg gaaaaggtca tagatggaaa ttactctata gagccacttt
     1261 ctaaaaaagt actgggaatg ataagggtaa gtagagggga acagaaatta ttcatcatta
     1321 actggcagtt agatggaaag agctataaaa atcattatct tttagggaat ccaccattca
     1381 gtctggaaag atatacagat tggatgggta agatattatg aaaccaggag gaatctatga
     1441 gtaagaaatt caatctccgt atgattggta atgcccatat agaccctgtg tggttatgga
     1501 aatgggaaga ggggttggaa accaccagag ccacatttaa gtctgccatc gacaggctgg
     1561 aagaatatcc agaatttgtc tttactgcat ccagtgcctg cctgtaccga tggatagaag
     1621 agaccgaccc ggaacttttt gaaaagatca gagagaaggt aaaggagggg aggtggtgta
     1681 ttacaggtgg atggtggata gaacctgact gcaatatacc atccggggaa gcactggcaa
     1741 ggcagggtct ctatggacag aaatattttg aggaaaagtt cggagtaaag gctaaagttg
     1801 gctataatcc agactctttc ggtcacaact ggatgatacc tcagatactt aaaaagatgg
     1861 ggatagatta ctacgtattt atgagaccag gaccgcacga gaaagaatta ccaggcgaca
     1921 tattctactg ggaatcccct gatggatcaa aggttcttac ctacaggatt ccattctcct
     1981 atgcaacctg gggtaaggat gtggaaaagc aggtaagaaa agtggcagaa aagcttgatg
     2041 agaatatcag ttctcttatg tgcttctatg gggttggaga tcacggtgga ggacctacaa
     2101 gggagaatat agagaatatc attgagttga atagaagaga agatatacct gaacttatct
     2161 tcagttctcc tgataggtat tttgagagac tgatctctaa aaagagagaa tttccagttg
     2221 taaaagatga tcttcagcat catgcaagtg gatgttactc tgtattatcc tggataaaaa
     2281 agacgaacag aaaactggaa catctactct tgaacagcga aaagattgca gtactgggaa
     2341 accttataaa aaatagacct tatcccagga aagaaatcac ttcaggctgg aagacattac
     2401 tatttaccca attccacgat atacttgccg gaacaagtat accagaggcg tatgaagaga
     2461 taaaagccat gcatggagcc gtagaggata atactaccag tatagtgata tacactactc
     2521 agagtatagc taaagaaatt gatacctcag gagagggaga accgataatt gtattcaata
     2581 cgacatcgtt tgagagaaat gtgccagtag agattgaact accctggaga gaagagaatt
     2641 tgagtattct agatgaaaat aagttgcctg taatatccca gctttccaga ccatcagcaa
     2701 ctaccgctgg aggaagggtc aaagtttcat tcattgcaga ccttccaccc ctgggttata
     2761 aggtatacag agcagtaccg gcagatattt ctataccgta taaacactct ctcaacataa
     2821 aagaaaatat cctggagaac gactggttat atctggagat agagagtgat acaggttata
     2881 taaaaagact ctatgataaa gagaataaga gagatatatt cagaggagaa gcagcgattc
     2941 cggtggtgat aaatgataca agcgatacct ggagtcataa cgtttttaaa ttcaaggaag
     3001 agattgggag cttttccaat gcaaaaatta aaataacaga gaacggtccc gataagggaa
     3061 caatcagggt agagaacttc tacaatagct ccaccgttat acaggaattt acactgtaca
     3121 gagaattgaa atctgtcgag gtaaaggtta ctgttaactg gaatgagaaa ctcaagatgc
     3181 tgaagctcag atttccgatc aatatagagg aacccaatgc caccttttct atcccatacg
     3241 gatttatctc cagaccctgt aatggggagg aagaaccagg tggagagtgg gtagacgcag
     3301 gtaatggtaa ttatggatta agtctgatta atgacgctaa gtacagttat gatatggatg
     3361 gtaatgtcct gtctcttaca gtactgagga gtcctgtata tgctcatcat ataccgagag
     3421 aattagaccc caatgag
//
LOCUS       DAOTKU010000140         3418 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_22969_length_3418_cov_3.759738, whole genome shotgun sequence.
ACCESSION   DAOTKU010000140 DAOTKU010000000
VERSION     DAOTKU010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3418
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22969_length_3418_cov_3.759738"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..880)
                     /locus_tag="P5310_04345"
     CDS             complement(<1..880)
                     /locus_tag="P5310_04345"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HRU32622.1"
                     /translation="MSRTVIKYGKVVIPGEGIVGEKDILIEDGVIKEISVGIPSDDTQ
                     VIDAENCYVIPGIVDIHVHLRDPDGDEEGITSGTSACSKGGVTTACCMPNTNPCIDNP
                     AIVRYIVLSAEKYGKIRVYPIGAMTVGREGKILAPYERMKSEGIVAVSDDGNWVEDPL
                     VMKSVLEMSNMEGLLPISHCEDKRVSLDGVVNEGIASFKLGLPGIPPLAETLAVARDT
                     VIARETGARLHIAHISLKGSLDIIRIARSSGVKITCEVSPHHFILTDDLLLEGGIGLK
                     VNPPLREEMDKLALIDG"
     gene            complement(877..2406)
                     /locus_tag="P5310_04350"
     CDS             complement(877..2406)
                     /locus_tag="P5310_04350"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HRU32623.1"
                     /translation="MLEYCIGIDAGTTNVKVILFDIDGNIVSSVSNPTPVWIDGKHYY
                     FLPDKIWEIIKDSIRKVSAEVNGNIASISATSVAESMVPIDENGDILFPAIAWYDERT
                     VEQLEWLLEKIDPQTIYSITGLRVQPIYGINKILWMKQYKPDIYRRAKVWLPVSDFIT
                     YMLSGEIATDYSQASRIMAFDLRSLRWSNFILDKAEIPASILPRPVPSGTLLGRVRNP
                     EEIGVKPDTVVAVGGHDHVCGAFINGCFRKGIGLDSMGTAESFQLSTEVPPLHLEVKE
                     RADITVGAHVSRGKYYIHVAIPFSGGLIEWSAKLMNSLGNMGDKILFDEFSSLAESSP
                     VGSNGLFCLPHFLGSSVPRRDPQARGVFLGLKREHLPGDFARSVLEGLSFEARLAFNT
                     LEEFGSIEKVIGIGGGTKNLTWLKIKSTVIDKVIEVPQVTEGTAMGAAFLGALGAGLF
                     KSEDEVVDRTYKVKIAVEPDKKLVPVYKELFDNIYTKIFYAVNDIDHLIDQEARKLWS
                     K"
     gene            complement(2426..2953)
                     /gene="hslV"
                     /locus_tag="P5310_04355"
     CDS             complement(2426..2953)
                     /gene="hslV"
                     /locus_tag="P5310_04355"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012963915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HRU32624.1"
                     /translation="MRHTTILAIKKGNTVAMGGDGQVTAGETVIKKRARKVRKIRDGK
                     VLVGFAGSAADGIALLDRFEAKLNEYGGNLARAAVELAKEWRLDRALRRLDALLVVAD
                     TNNLLLISGTGDVIEPDDGIIAIGSGGNYALAAARALIKYTDLSCEEIVRESLSIAAD
                     ICIYTNNEFIIETLS"
     gene            complement(2955..3383)
                     /locus_tag="P5310_04360"
     CDS             complement(2955..3383)
                     /locus_tag="P5310_04360"
                     /inference="COORDINATES: protein motif:HMM:NF024947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HRU32625.1"
                     /translation="MENIINRASLELNFLTQGRYSFSLASDDSADIMVKDNWYPERSR
                     KTYSLSGGESFLASIALAIAIAEEIRGKRSLDCLFIDEGFGSLDDTGLDSIVSALAEL
                     ENSGIMIGVITHNRELASRFPYRIEVEKDERGSRIKEGGW"
BASE COUNT          916 a          915 c          584 g         1003 t
ORIGIN      
        1 atccatctat aagagcaagc ttatccatct cctctctcag cggaggattt acctttaaac
       61 cgatcccccc ttcgaggaga aggtcatcag ttaatatgaa atgatgtgga gagacctcgc
      121 aggtaatctt tacccctgat gacctggcta tccttataat atccagggac cccttaaggg
      181 atatatgcgc tatatggagt ctcgcacctg tctctctggc tataactgta tcccttgcaa
      241 cagccagcgt ctctgctaac ggaggaatac caggtagacc cagcttaaaa gatgctatcc
      301 cctcattaac aacaccatcg agagagaccc ttttatcctc acagtgagag atgggaagta
      361 atccctccat atttgacatc tccagcactg acttcataac cagcgggtcc tccacccagt
      421 ttccatcatc ggatacagcc acaatccctt ctgatttcat cctctcataa ggggcaagaa
      481 tctttccctc tcttcccact gtcatagctc cgataggata cactcttatc ttcccatatt
      541 tttctgccga aaggacaata taccttacta tggcaggatt atcaatgcag ggattcgtat
      601 ttggcataca gcaggcggtg gtcaccccac ccttactgca ggcagatgtt ccagaagtta
      661 taccctcttc atctccatcc gggtctctca aatgcacatg aatatcaact atgcccggga
      721 ttacatagca attctcagca tctatgacct gtgtatcgtc tgacggtatg cctacagaga
      781 tctcctttat aactccatct tctatcagga tatctttttc tccaacaata ccttctccag
      841 gtatgaccac cttaccatac tttataactg tcctgctcat tttgaccaca gctttcttgc
      901 ctcctggtct ataagatggt caatatcatt aacagcatag aatatcttcg tataaatatt
      961 atcaaagagc tccttatata ccgggaccag ttttttatca ggttctactg ctatttttac
     1021 cttatatgtc ctgtccacca cctcatcctc actcttaaat aacccggcac ccagagcccc
     1081 taaaaaggca gcccccatag cggttccctc tgttacctgt ggaacttcta taaccttatc
     1141 aatcactgtc gattttattt tgagccaggt taaattcttt gtccctcccc ctatccctat
     1201 aactttctct atagacccaa attcctccag ggtattaaag gcgagcctcg cctcaaaaga
     1261 aagtccttca aggacacttc tagcaaaatc accaggtaga tgttccctct ttagcccaag
     1321 gaaaacccct cttgcctgtg ggtctctcct ggggacactg ctaccaagga aatgtggtag
     1381 acaaaagaga ccgttacttc caacaggaga gctctctgcc agagaagaaa attcatcaaa
     1441 cagaatttta tctcccatat tacccagaga attcataagt ttagctgacc attctataag
     1501 cccgccagaa aatggaatgg ctacatgaat ataatacttt cccctggaaa catgagcacc
     1561 tacagtgata tctgccctct cctttacctc aagatgtaag gggggaacct cagtactcaa
     1621 ctgaaaactc tccgcggtac ccatagaatc aagcccaatc cctttacgaa aacatccgtt
     1681 tataaaggca ccacagacat ggtcatgtcc gccaactgcc actacagtat caggtttgac
     1741 tccaatctct tccggattcc tcaccctgcc gagtaaggtt ccactcggca caggtctggg
     1801 gagtatgctg gcaggtatct ctgccttatc cagtataaaa tttgaccatc gaagtgacct
     1861 caaatcaaaa gccattatcc ttgaagcctg agaatagtca gtagctatct caccagagag
     1921 catatatgtt ataaagtctg atacgggaag ccataccttt gccctcctgt agatatcagg
     1981 tttatactgt ttcatccata gaatcttatt tataccatat atgggttgaa cccttaaacc
     2041 agttatgctg tatatggtct gagggtctat cttctccaat agccattcta actgttcgac
     2101 cgtcctttca tcataccagg cgatagcagg gaagagtata tctccatttt catctatggg
     2161 gaccatcgat tctgccacac tggtggcaga tatacttgca atatttccat ttacctctgc
     2221 cgatactttc cttattgaat ctttgataat ctcccatatc ttatcaggta aaaaataata
     2281 atgcttgcca tcaatccaga ccggagttgg attagaaaca gaagagacta tattgccatc
     2341 aatgtcaaag agtatgacct ttacattggt ggtaccggca tctatgccta tacagtattc
     2401 caacatcctc acctctttct atgaattacg atagagtttc aattataaac tcattattgg
     2461 tatatataca tatgtctgca gctatcgata gtgattccct cactatctcc tcgcaggata
     2521 agtcagtata ttttatcagg gctctggctg cagccagagc ataattgccc cctgaaccaa
     2581 tggctattat accgtcatct ggttctataa catcccctgt accggagatt aagagcaggt
     2641 tatttgtatc ggctaccacc agtaatgcat ccagccttct cagggctcta tcaagtctcc
     2701 actctttagc cagttccaca gcagccctgg ctaaatttcc tccatactcg tttaatttag
     2761 cctcaaatct atcgagcagt gctatcccat cagctgctga accagcaaaa cctaccagaa
     2821 ctttgccatc ccttattttt ctgaccttcc ttgccctttt ctttataacg gtctcgccag
     2881 cggtaacctg tccatctcca cccatagcta ccgtgtttcc cttttttata gccagaattg
     2941 tagtgtgtct cacactacca gcctccttct tttatcctgg agcctctctc gtccttctct
     3001 acctctatcc ggtatgggaa tctacttgcc agctctctat tatgtgttat aactccgatc
     3061 ataataccag agttctccag ctccgccagg gcagagacta tgctgtcaag tcctgtatca
     3121 tccagagagc caaaaccctc gtctataaaa aggcaatcca gagacctttt acctcttatc
     3181 tcttctgcta tagctatagc cagtgctata gacgcgagaa aactctcccc tcctgaaaga
     3241 gagtacgtct tacggcttct ctcaggatac cagttatctt ttaccatgat atcggcagag
     3301 tcatctgagg ctaaagagaa agagtatctt ccctgtgtga ggaaatttag ctctaaagag
     3361 gctctattta tgatattttc cattagaagg tgcatttata tatatgtatc cacctagt
//
LOCUS       DAOTKU010000141         3416 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_22987_length_3416_cov_2.845582, whole genome shotgun sequence.
ACCESSION   DAOTKU010000141 DAOTKU010000000
VERSION     DAOTKU010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3416
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22987_length_3416_cov_2.845582"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(87..962)
                     /gene="truB"
                     /locus_tag="P5310_04365"
     CDS             complement(87..962)
                     /gene="truB"
                     /locus_tag="P5310_04365"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HRU32626.1"
                     /translation="MVNVVKPPLITSFKVVEIIKRKFKLRHVGHLGVLDPIATGLLQI
                     AVGSATKLFEYFTGLERSYRAELLLGISTDTQDAAGRPIKISEDVDISNLEEIIDSFT
                     GEITQIPPMYSSLHYKGKRLYELARDGIEVPRTPRNVLIKHIDIIDIKKDRYPRIILD
                     VVCQRGTYIRTLCHDIGEKLGVGAHMLSLVRTRIGNIDLSQAYSLTEILGRGNSIAEY
                     FMPWGMLSEIEKIDLIVKKQRDDFIKGKPILLYKTYYSRREFVSVWYNNECIGVGRCF
                     QDVLYPIAVENLNVS"
     gene            complement(968..1930)
                     /locus_tag="P5310_04370"
     CDS             complement(968..1930)
                     /locus_tag="P5310_04370"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HRU32627.1"
                     /translation="MKAEYIPNQSKIIKLIQEKKSFLIVSHVYPDGDAIGSVLGISNA
                     LISLDKKVVAYIADRIPRQFSSLPGIDLVRTKYNVEETDATFVLDASNIERLGEAGNF
                     ISGSIINIDHHPDNSLFGDVNLVIPTASSTSEIVFNLLKYFPLNKEAVSALYYGLLSD
                     TGGFMYNNTGEGTFKTALDMIRYGVPAGEIAYKVFYSIPYATMKIYIDVLKDIHIDKE
                     VNLVWCILPLEVMESEAFQNNTSPLLDMLLKIEEADTIVLFKEHKDKYRVSLRSKSKD
                     VGAIARALGGGGHRSASAFDLLNKQESAIIDTLNKIKTLLGGQN"
     gene            complement(1920..2270)
                     /gene="rbfA"
                     /locus_tag="P5310_04375"
     CDS             complement(1920..2270)
                     /gene="rbfA"
                     /locus_tag="P5310_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HRU32628.1"
                     /translation="MKPSRLERLEALVKEEVSDIILKMKDPRIKFVTVTDVTISKDVK
                     YAKIFISIMGEEEEKKNSFSAIKGATGFIRTELAHRLEIRRIPELSFVLDDSIERGVR
                     LANYIEKIVHSDES"
     gene            complement(2292..2573)
                     /locus_tag="P5310_04380"
     CDS             complement(2292..2573)
                     /locus_tag="P5310_04380"
                     /inference="COORDINATES: protein motif:HMM:NF016351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HRU32629.1"
                     /translation="MMIGVCKIELYIPGALSLKDKRRYLQGLIKRLRNNFNVSVVEID
                     YKDKWQRTLIGIACIDERQKEIDMNFSKMINYISKERDIEVVDYSIEMF"
     gene            complement(2579..>3416)
                     /locus_tag="P5310_04385"
     CDS             complement(2579..>3416)
                     /locus_tag="P5310_04385"
                     /inference="COORDINATES: protein motif:HMM:NF023413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HRU32630.1"
                     /translation="MYGKVRALLNDKGERIKKATPSMPVQIVGLPRLPVAGENFTIVE
                     NERMAKELISQWGEETQQQREVLSLENIRLRIDKGELKDLNLVLKADTQGSLEAIEKF
                     LKEINIEGVTLSIVRRGVGNISEGDVLLAKASKAIVVGFNVETELGILKLAENEGVSI
                     KIYNIIYELIDDITLALKGLLKPEKQELILGRAEVRQVFKSSRIGSIAGCYVLDGKIV
                     RNSNIRIKRADNVIYEGRLTSLKRFKEDVREVTSGYECGIFIEGFEDYKEQDVIESYT
                     FE"
BASE COUNT          945 a          768 c          488 g         1215 t
ORIGIN      
        1 atagtatggc ttgatgtcct ttatgaaccc catcaaatac acccagagta ataacagtag
       61 ggtcttttat ctgtgcagta tcccattcaa ctaacattta aattctcaac tgctatcggg
      121 tatagtacat cctggaaaca tctacctacc cctatacact cattgttata ccatacagag
      181 acaaactctc ttctggagta atatgttttg tatagcagta tgggtttacc ctttataaaa
      241 tcatcacgtt gtttctttac tataaggtct attttttcta tttcacttaa catcccccag
      301 ggcataaaat actcggcaat agagtttccc cttccaagta tttctgttaa ggaataagct
      361 tgagatagat ctatattccc tattctggtt cttaccagag agagcatatg tgccccaact
      421 cctaatttct ctcctatgtc atggcatagt gtccttatat atgttcctct ctggcatacc
      481 acatcaagta taatcctggg atatctatct ttctttatat ctattatgtc tatgtgtttt
      541 ataagaacat ttctaggtgt tcttggaact tcaataccat ctctggcaag ttcatacagt
      601 cttttacctt tatagtgcaa tgaagaatac atgggaggaa tttgagttat ctctcctgta
      661 aagctatcta taatttcctc cagatttgaa atatcaacat cctcggatat tttaattggt
      721 cttccagcag catcctgagt atcggtagat atacctaaaa gcagctctgc tctataggac
      781 ctttccagcc cggtaaaata ttcaaatagt tttgtggctg accctacagc tatctgtaat
      841 aatcctgtgg ctatagggtc aagcactccc agatgtccta catgtctgag tttgaattta
      901 cgttttataa tttctaccac cttgaaagat gtaattagtg gtggtttaac tacattaacc
      961 accccgttca attctgccct cctaatagag tcttaatctt atttagagta tcaataattg
     1021 cagattcctg tttgtttaaa agatcaaaag ccgaagcact tctatgtccg cccccaccta
     1081 aagctctggc tattgctcca acatccttac ttttactcct caggcttact ctatatttat
     1141 ctttatgttc tttgaaaagg actattgtgt ctgcttcttc tatttttaga agcatatcaa
     1201 gtagaggaga tgtattgttc tggaatgcct cactttccat aacttctagt ggaagtatac
     1261 accacactaa gtttacctct ttatctatat gaatatcttt aagcacatcg atatagatct
     1321 tcatggttgc atacggtata gaatagaaga ctttatatgc gatttctcct gcaggaaccc
     1381 catatcttat catatccagg gcagttttga atgtcccttc acctgtgttg ttatacataa
     1441 aaccacctgt gtctgagagt aaaccataat acagggcaga taccgcctct ttattcagcg
     1501 ggaaatattt taaaagatta aagactatct cactggtact tgaagctgtg gggataacca
     1561 ggtttacatc accgaacagt gaattgtcag gatggtggtc tatattaatt atcgatccag
     1621 aaataaagtt gccagcctct ccaagccttt caatattact tgcatccaaa acaaatgttg
     1681 catctgtctc ttccacgtta tatttagtcc ttacaagatc tatacctgga aggctggaaa
     1741 attgccttgg aatcctgtcc gctatatatg ctactacctt tttgtcaagt gatataaggg
     1801 catttgaaat acccaaaaca gaaccgatag catctccatc gggatacaca tgagatacta
     1861 tcagaaagct cttcttctcc tgtatcagtt taataatttt tgattggttt ggtatgtatt
     1921 cagctttcat cactgtgtac aatcttctct atataattgg ccagtcttac ccctctttca
     1981 atcgaatcat ccagtacaaa agataactca ggtattcttc ttatctctaa cctatgggca
     2041 agttcagttc ttataaagcc agtggctcct tttattgcgg agaacgagtt tttcttttcc
     2101 tcctcttccc ccataatact gatgaatatc tttgcatact ttacatcctt agatatggtt
     2161 acatctgtaa ctgtgacaaa ttttattctg gggtctttca tctttaatat tatatcgctt
     2221 acttcttctt ttaccaatgc ctctaatctt tccaatctag agggtttcat aatacatcct
     2281 ccattaagtt attaaaacat ctctatagaa taatctacaa cttcgatgtc tctttctttc
     2341 gagatataat tgatcatttt agagaaattc atatctatct ctttctgtct ttcgtctata
     2401 caggctatgc ctataagtgt tctttgccat ttatctttat agtctatttc aaccacagat
     2461 acattaaaat tatttctcag cctcttaatg agaccctgaa gatatctcct cttatcttta
     2521 agacttaacg caccaggtat gtataattct atcttacaca cccctatcat catatagctt
     2581 attcaaacgt ataactttct ataacatcct gttctttata atcttcgaaa ccttctataa
     2641 atatgccaca ctcatacccg gaggttactt cccggacatc ctctttaaat ctttttaaag
     2701 aggtaagtct cccttcatag attacattat ctgctctctt tatcctgata ttactatttc
     2761 ttacaatctt tccatctaaa acatagcaac ctgctatact tcctatcctt gaagacttga
     2821 agacctgacg gacctctgct cttcccaaga ttaattcttg cttctcaggt tttagaagcc
     2881 ctttaagggc aagagttata tcatctataa gctcatatat aatattatat atctttatag
     2941 atacaccttc attctctgcc agtttaagaa ttcccaattc cgtttctacg ttaaatccaa
     3001 ctactatagc cttagaagcc ttggcaagaa gcacatcccc ttcagatata ttaccaaccc
     3061 ctcttcttac tatagaaagg gtaacccctt caatatttat ttcttttagg aatttttcta
     3121 tagcttctaa agaaccctgg gtatctgctt tcagtacaag atttaaatcc tttaactctc
     3181 ctttatctat tcttagcctt atattttcca gagagagaac ttctctctgt tgctgtgttt
     3241 cctcccccca ctgggatatt aattcctttg ccatccgttc attttccact attgtgaaat
     3301 tttccccagc aacaggaagc ctgggtaacc cgactatctg cacaggcata gatggggtag
     3361 ccttcttaat tctctcccct ttatcattga gaagagctct tacctttcca tacatt
//
LOCUS       DAOTKU010000142         3399 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_23136_length_3399_cov_3.816687, whole genome shotgun sequence.
ACCESSION   DAOTKU010000142 DAOTKU010000000
VERSION     DAOTKU010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23136_length_3399_cov_3.816687"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..610)
                     /locus_tag="P5310_04390"
     CDS             complement(<1..610)
                     /locus_tag="P5310_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011916701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRU32631.1"
                     /translation="MIQFWGDSIPGYDQSINDPEAPSLEPYLLDNGRENSVIIVFPGG
                     GYTKRAAHEGTPVALWLNSIGVSSFVLHYRVAPYRYPYPLLDAKRAIKFIRFNSDTFK
                     INPDRIGILGFSAGGHLACMCATLPDYGENNPDDPVEKVGSKPNALILCYPVISFKSF
                     YHKGSMVNLLGENPPDELRELLSGENNVSKDTPPTFIWHTSDD"
     gene            771..2078
                     /locus_tag="P5310_04395"
     CDS             771..2078
                     /locus_tag="P5310_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013180932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HRU32632.1"
                     /translation="MIWSEYEKLSRREYKDLQLENLKRTVERVYENVPFYRKKFDEIG
                     LKPHHIKKLEDIRHIPFTTKDDLRNNYPYGLFAVPLTKIVRLHASSGTTGKPTVVGYT
                     KRDMEIWAEVVARMVTEAGVREYDIAQIAFGYGLFTGAFGLHQGLEKVGATVIPVSSG
                     NTEKQIMIMQDFGVTVLVCTPSYALYMDEVASELNVDRDKIKLRIGLFGAEGSTVEMR
                     REIEKKWGLFATENYGLSEIIGPGISGECEYRDGLHINEDHFYPEIIDPDRGEILEEG
                     EEGELVLTSITKEGMPLLRYRTHDITSLIYEPCRCGRTNVRMTAVKGRTDDMLIIRGV
                     NVFPSQIESALIGIEGIGPHYQLVITRNGYLDDLEVHVELVDGKLLERYSELEKLEEK
                     IKHRIYTFLGLNVKVKLVEPKTLERTTGKAKRVIDLRHGGQKL"
     gene            2075..>3399
                     /locus_tag="P5310_04400"
     CDS             2075..>3399
                     /locus_tag="P5310_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015907959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRU32633.1"
                     /translation="MKRLMLGNHAIARGCFDAGVRVATAYPGTPSTEITEYISQYDEI
                     YSEWAPNEKVALEVALGAAIFGKRALCSMKHVGLNVASDPLFTASYTGVNAGLVVAVA
                     DDPGMHSSQNEQDTRNIAKAAKIPVLEPSDSQECLDFARRGFEISEMFDTPVVLRSTT
                     RVSHSQSIVEIDERKEVDFEYKKDVEKYVMMPAMARKRHEIVEEREKRLREFAETVEL
                     NRIEEGTSNMAFITSGISYQYVKEAYPDACILKLGMVYPLPEKLVRGFCGRFEKVYVV
                     EELDPFLEENIKVMGVNNIIGKEIFKRTGEYSPSFIKTSIENCKLEIPYRIDEELPAR
                     PPVLCPGCPHRGVFYSLRRIKNAVITGDIGCYTLGALPPFNAMDTCICMGASIGMAHG
                     ISKVSDPSIKTIAVIGDSTFIHSGITGLIDAVYNKSNILILILDNITTG"
BASE COUNT         1083 a          569 c          814 g          933 t
ORIGIN      
        1 gatcgtctga agtatgccat ataaatgttg gcggagtatc ctttgaaaca ttattctctc
       61 cggatagaag ctctcttaac tcatctgggg gattttctcc aagaagattc accatggagc
      121 ctttatgata aaaacttttg aaacttatga ccggataaca gaggataagg gcgtttggct
      181 ttgaacccac tttttctaca gggtcatctg gattattctc tccatagtca ggaagggttg
      241 cacacataca tgccagatgt cctccggcag aaaatccaag gattcctatc ctgtcaggat
      301 ttatcttaaa cgtatcactg ttaaatctga taaattttat ggctctttta gcatctagta
      361 gagggtaagg atacctatat ggagctactc tatagtggag gacgaatgaa gaaactccga
      421 tactgttaag ccagagtgca acaggtgtac cctcatgagc agctcttttt gtatatcctc
      481 ctccaggaaa tacgataata acactatttt ctcttccgtt atccagaagg taaggttcaa
      541 gagatggggc ttcagggtca tttatacttt gatcataacc ggggatacta tcaccccaaa
      601 attgtatcat aaaatcacct ctttcgatta aggtcaagaa atctcctgta tattatacca
      661 gaagggagtc caattagttt gaaaataaat tagatttaca ctataataga ttccacttgc
      721 gaggacttaa aaatataaga tattacagat aggaaggaag gcaagctggt atgatctggt
      781 cagaatacga aaagcttagc agaagggaat ataaagacct tcaacttgaa aatttaaaga
      841 ggacggtaga aagagtctat gaaaacgtcc ctttctaccg taagaaattc gatgagattg
      901 gtttgaaacc acatcatatc aagaagctgg aagatatccg tcatattcca tttacaacaa
      961 aggatgatct gagaaataat tatccttatg ggttatttgc agtacctctt acaaagattg
     1021 taagattgca tgcctcttcc ggtacaacag gtaaacccac cgtggttggt tatacaaaac
     1081 gggatatgga gatctgggca gaagttgttg caaggatggt tacagaggca ggggtaaggg
     1141 agtatgatat tgcccagatt gcatttggtt atggactttt tacaggtgca ttcgggcttc
     1201 atcaggggtt agagaaagtt ggtgcaactg taattccagt ctcaagtgga aatactgaga
     1261 aacagattat gattatgcag gattttggtg taactgttct tgtctgtact ccatcctatg
     1321 ctctctatat ggatgaggtt gcttctgaat tgaatgttga tagagataag ataaaactca
     1381 gaattggtct ttttggagca gaaggatcaa ctgttgagat gagacgtgag attgaaaaga
     1441 aatggggtct ctttgcaaca gaaaattatg ggctttctga gattatcgga ccaggtatct
     1501 ctggcgagtg tgagtataga gatggacttc atataaatga ggaccacttc tatccagaga
     1561 taatagatcc ggatagagga gagatactgg aagaagggga agagggagag cttgttttaa
     1621 cttcaattac aaaagagggt atgccacttc tgaggtatag gacacatgat ataacttcat
     1681 tgatctatga accctgcaga tgtggacgta caaatgtaag gatgacagct gtaaaaggac
     1741 gaaccgatga tatgctgatt attagaggtg taaatgtatt cccctcacag atagagagtg
     1801 ccctgattgg gatagaaggg attggtcctc actatcaact ggtaataacc agaaatggct
     1861 atcttgatga cctggaggtc catgtagagc tcgtggatgg taaattactc gagaggtatt
     1921 cagaacttga gaaactggaa gaaaagataa aacacagaat ttataccttt cttggtctaa
     1981 atgtaaaggt aaaacttgtt gagccaaaga cactggaacg gacaacaggt aaggcaaaaa
     2041 gggttatcga tctgagacat ggaggacaga agctatgaag agacttatgc ttggtaatca
     2101 tgcgattgca agaggatgtt ttgatgctgg ggtcagagtg gcaactgcat atcctgggac
     2161 accttcgaca gagattacag aatatatctc ccagtatgat gagatttaca gcgaatgggc
     2221 accaaatgaa aaggttgcat tggaggtagc tcttggtgca gctatcttcg gtaaaagagc
     2281 tctctgctca atgaagcatg taggtcttaa tgtcgcatca gacccgttat tcacagcatc
     2341 gtatacaggc gtcaatgctg gtctggtagt tgcagttgcc gatgaccccg ggatgcattc
     2401 gtctcagaat gaacaggaca caagaaacat agcgaaggct gcaaagatac ccgttttaga
     2461 accatctgat agccaggaat gtctggactt tgccaggcgc ggatttgaga taagtgagat
     2521 gtttgacact cctgttgtcc tgcgatctac aacaagagtt tctcattcac agtcaatcgt
     2581 ggagattgat gaaaggaaag aggtcgattt tgaatataaa aaggatgtag agaaatacgt
     2641 aatgatgccg gcaatggcaa gaaaaagaca tgagattgtg gaggaaagag agaaaaggtt
     2701 aagagagttt gcagagacag ttgaactgaa tagaatagaa gagggaacat caaatatggc
     2761 tttcattaca tcaggaataa gctatcagta tgtgaaggag gcttatcctg atgcctgtat
     2821 tttgaagctt ggtatggtct atccgctacc agaaaagctg gtaagaggtt tctgcggtag
     2881 atttgagaag gtgtacgtag ttgaggagct tgatccattt ttagaggaaa atattaaagt
     2941 aatgggggtc aataatataa tcggtaagga gatatttaaa aggacaggtg aatattcacc
     3001 atcttttata aagacctcaa ttgaaaactg taaacttgag ataccgtaca ggatagatga
     3061 ggaattacca gcaagacctc cagtactgtg tccgggttgt ccacatagag gggtcttcta
     3121 ttccctcaga aggataaaaa atgctgtgat tacaggagat atcggatgtt atacgctggg
     3181 tgcccttcca ccattcaatg ctatggatac ctgtatatgt atgggggcaa gcatagggat
     3241 ggctcatgga atctcaaagg tatctgatcc gagtataaag accatcgcag tgataggaga
     3301 ttctaccttt atccattccg gtataactgg acttatcgat gctgtctata ataaatccaa
     3361 tatcctgata ttgattttag ataacataac aaccggaat
//
LOCUS       DAOTKU010000143         3386 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_23258_length_3386_cov_3.143801, whole genome shotgun sequence.
ACCESSION   DAOTKU010000143 DAOTKU010000000
VERSION     DAOTKU010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3386
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23258_length_3386_cov_3.143801"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(189..1163)
                     /locus_tag="P5310_04405"
     CDS             complement(189..1163)
                     /locus_tag="P5310_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRU32634.1"
                     /translation="MSFELPEDRTLVIPLLGAPGVKLSNTTLKENLTDEDIQLRTIKL
                     LAEKFQPDGIFPFMDLTVEAEALGLDINFSENENPAVRDHSIKTEEDLESVRLRYRGI
                     SGRMPLFIKVVEGLSKDLSIIKGAYTIGPFTLAGELMGVGELSMNVILNPDLAHKFIE
                     FTTSVIKDYAEALLDAGADIIAVLEPSAVMLSPKQFEEFSGRYFSRLVRELKVPLILH
                     ICGDTTHLLEKMSETGAVGLSLDSLVDLKEASRVVPGSIALIGNLDPVGVFLRSTPEE
                     VAIATRNLLEKMRGVNNFILSSGCDLPIDTPLENIEAFMRVGRDSGVR"
     gene            complement(1167..1805)
                     /locus_tag="P5310_04410"
     CDS             complement(1167..1805)
                     /locus_tag="P5310_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HRU32635.1"
                     /translation="MVNLQEISEALIVGNAKKVRELVEQALKEGVSPKEILDNALVPG
                     MNVVGEKFKNNEYYVPEVLIAARAMHAGMDLIKPLLTQTGEAYKGKVVIGTVRGDLHD
                     IGKNLVAMMLEGAGFQVIDLGVDVPPDKFVDTAKNEGANVIAMSALLTTTMLAMKDTI
                     NVLKEAGIRDRVKVMIGGAPVTQRYADEIGADGYAPDAATAVEKAKELLGLN"
     gene            1914..2930
                     /locus_tag="P5310_04415"
     CDS             1914..2930
                     /locus_tag="P5310_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HRU32636.1"
                     /translation="MRALVYYGARDIRVSEVEEPEPGKGDILIRVSACGICGSDVHGY
                     LGITGRRIPPMIMGHEFTGIVADTGEDVNDISKGDRVTVYPTRFCGSCSFCKTGLTNL
                     CINRTVLGVMDVNGAMAEYVVVPKNTIIKLPNNIDPIQGTMLEPLGVVYRAVKNAPNI
                     LNNSVLIAGAGTIGLLLLQLVKLAGANPVIVTDINESRLDIAKKLGADIVLDPEREDI
                     KDALSKLNIDGVDVAFEAVGAESSVNQVLNSVKNRGTSILIGNSQRDITVNMQNIVTR
                     ELKIFGSYTYTIEEFKATMDVINRIDTESIISKVISLEESPRVFEELAQKSSNLLKVV
                     IRLD"
     gene            2991..3269
                     /locus_tag="P5310_04420"
     CDS             2991..3269
                     /locus_tag="P5310_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32637.1"
                     /translation="MAEILSLDINPTSGKQGDMVTAVVKATPDTKSVYGRIPEYGYML
                     QFRKEGDVFKLSMQIPYGAPPGVYMVSVYAVDNEGNRGEEKRFNFQVL"
BASE COUNT         1012 a          700 c          665 g         1009 t
ORIGIN      
        1 ggtttcttta aagacttaca ggaaggtgca gatcatatgg taaagataaa gagtgttata
       61 gatccaaatt tgaagaataa agatatttat aatttttact ttgataaata caggaaaact
      121 tatgatcaac tgaaagaact tatgcatgag atggtaagat ttaccactta ggataacaac
      181 tcgggggctt atcttacccc cgagtctctt cctacacgca taaaagcttc tatgttttcc
      241 agtggcgtgt ctatcggtaa gtcacaacca gaacttaaga taaaattatt aactcccctc
      301 atcttttcca gtaaatttct ggtagctatg gctacttctt ctggagtaga ccgtaagaac
      361 acccctacag ggtccaggtt tcctatcaga gcaatactgc caggtacaac tcttgaagct
      421 tccttcaggt caaccagact atccaggctt aatccaaccg ctccagtttc ggacattttc
      481 tctaatagat gagtggtatc tccacatatg tgaagtatga ggggaacctt taactctctg
      541 acaagacgag aaaaatacct tcctgaaaat tcttcaaatt gctttggtga tagcatcacc
      601 gcagagggtt ccagtaccgc gataatatct gccccggcat ccaggagagc ttcagcgtaa
      661 tcttttatca ctgatgtggt gaattctata aacttatgcg ccaaatcagg attcagtata
      721 acattcatgc ttagctcacc tacacccata agttcaccag ccagggtgaa tggtcctata
      781 gtataagccc ctttaattat agagagatct ttggacagac cttctaccac tttgataaat
      841 agaggcattc taccagagat acccctgtat ctgagtctaa cagattccag gtcttcctct
      901 gtttttatgc tatgatctct tacagcagga ttttcattct cagagaagtt tatatctaaa
      961 cccaacgcct ccgcctctac ggtaaggtcc ataaagggga atatcccatc aggctgaaac
     1021 ttctctgcca gcaattttat agtcctaagc tggatgtctt catcggtcag gttctctttt
     1081 aacgttgtat ttgaaagttt tacacccggc gctcctaaca gcggaattac taatgttcta
     1141 tcttcaggta attcaaaact catatattaa tttaagccta ataactcctt tgccttctcc
     1201 accgctgttg ctgcatccgg ggcatagcca tctgcaccta tctcatctgc atatctctgt
     1261 gttaccggag cgcctcctat cataactttt accctatctc ttatcccggc ttctttaagc
     1321 acattaattg tatccttcat agcaagcatt gtggtggtca aaagagctga cattgctatt
     1381 acatttgcgc cctcattctt ggctgtatcc acaaatttat ccggaggcac atccaccccc
     1441 aaatctatta cctgaaatcc agcgccttct aacatcatag ctaccagatt ctttcctata
     1501 tcgtgtaaat ctcctctaac tgtccctata actacctttc ctttataggc ttctcctgtt
     1561 tgagtcaaga gaggctttat caggtccatt cctgcatgca tagccctggc agctatcaaa
     1621 acctctggca catagtactc attattctta aacttttctc ccacaacgtt cattccggga
     1681 acaagcgcat tatccaggat ctctttagga gatacaccct cttttaacgc ctgttcgacc
     1741 agctctctca ctttcttagc attccctaca atcaacgctt cagatatctc ttgtagattt
     1801 accatctatt tacacctcca agggtatttt tcagaaattc taccatatat ataaagaaat
     1861 taaaagtgct ataatatcct gaaagtgttt aatctcaaaa ggcaggtgaa aaaatgagag
     1921 ccctggtata ctatggagca cgggatataa gagtttctga agttgaagaa cctgaaccag
     1981 gaaagggaga tattcttatc agagtaagcg cttgtggtat ctgtgggtct gatgtgcacg
     2041 gttatcttgg tattacagga agacgtatcc caccaatgat aatgggtcat gaatttaccg
     2101 ggatagttgc agatactgga gaggatgtca atgacatctc caaaggagat cgggtaaccg
     2161 tctatcctac caggttctgt ggtagctgta gtttctgtaa gactggttta acaaacctct
     2221 gtataaatag aactgtcctg ggtgtaatgg acgttaatgg ggctatggca gagtatgtgg
     2281 ttgttcccaa aaataccatt ataaaattac ctaataatat cgatcctatc cagggtacca
     2341 tgctggaacc actgggggta gtttacaggg cagtaaaaaa tgccccaaat atcctgaata
     2401 atagtgtcct catagctgga gctggaacca ttgggttact tctccttcag cttgtaaagc
     2461 tggcaggggc aaatcctgtc atagttacag atataaatga gagtcgtctt gatatagcca
     2521 agaagttagg tgctgatata gttttggatc cggagagaga ggatataaaa gatgctttat
     2581 ctaaattaaa tatagacgga gtagatgtcg cattcgaggc tgtaggagca gagtcatctg
     2641 ttaaccaggt attaaattct gtaaagaata ggggaacttc tatattgata ggtaattctc
     2701 aaagagatat tactgttaat atgcagaaca tagttaccag agaattaaaa atctttggaa
     2761 gctataccta tactatagaa gaatttaaag ctactatgga cgttataaat agaatcgata
     2821 ctgagagtat tataagcaag gttatttcat tagaagaatc tcccagggta ttcgaagaat
     2881 tagcccagaa gagcagtaat ttactcaaag tggtaatcag gctggattga caaagaaaaa
     2941 atgagatgat aatataaggt acagaaaact gattgggagg aggtagtatt atggcagaaa
     3001 tattaagttt ggatataaat ccaacatctg ggaaacaggg agatatggta accgcagtag
     3061 ttaaagccac tccagacaca aagtctgttt atggcaggat ccctgagtat ggatatatgc
     3121 ttcaatttcg aaaagagggg gatgtcttta aattgagtat gcagatcccc tacggagcac
     3181 ctcctggggt atatatggtc tccgtatatg ctgtagataa cgaaggtaac agaggcgagg
     3241 agaagaggtt taattttcag gtcctgtaaa ttcagataaa gatatcccaa gagccttaag
     3301 ggattgaaat atctctcctg ctctctcttc ttttttacat aattcaatct cttttctgat
     3361 cctggcaatt cccactgtcc ttaagg
//
LOCUS       DAOTKU010000144         3373 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_23393_length_3373_cov_3.574744, whole genome shotgun sequence.
ACCESSION   DAOTKU010000144 DAOTKU010000000
VERSION     DAOTKU010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23393_length_3373_cov_3.574744"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="P5310_04425"
     CDS             <1..325
                     /locus_tag="P5310_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HRU32638.1"
                     /translation="RATRYGGTIVPTSFYHGEAEELNLGEEWHLNRQVMVSGARVESE
                     PYRDYPRWDRTRVYNTVIELFLLKKLSVEGFLHIVDFEDVLEAYQTLEKEPNKWIKLG
                     VRYGR"
     gene            543..1319
                     /locus_tag="P5310_04430"
     CDS             543..1319
                     /locus_tag="P5310_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRU32639.1"
                     /translation="MGKIIAVANQKGGVGKTTTVINLGFALSELGKRVLLVDGDPQGN
                     STSGIIQKRNIKPNLYDALICDVSVKEVILPLKDGKTSIRDNISLIPSNLELAGAEIE
                     LVPVFLRELRLKKVLEPVKDDYDYIFIDCPPSLGLLTVNALSASQYVLIPLQCEYYAL
                     EGISQLLKTIELIKKDLNPALEILGILLTMYNRTILAQQVLDETKRFFKDKVFNTLIP
                     RNVRLSEAPGFSLSIFEYAPDSSGAGAYRELARELMNIGK"
     gene            1309..2124
                     /locus_tag="P5310_04435"
     CDS             1309..2124
                     /locus_tag="P5310_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HRU32640.1"
                     /translation="MENKRLGRGLSALIPSEEKKKGVEEIEVDRIVPNPNQPRESINE
                     ENIRELAESIREKGILQPILVRRKDDRYYEVVAGERRYLSAKLLGLNRVPAIILDIDE
                     KEAYEISLVENIQREDLNPIEKAEAFKDYIDKYRVTHEELAGKIGISRTEITNLLRLL
                     QLPQEIKEEVKKGTLTYGHARTLLSLESIDSQKEIAEKIINERLSVRETEELVSKKRE
                     IREIPEIRALESKLQNYLETRVKIQPQSPERGRITIEYKSLEELERIIGKFLE"
     gene            2121..2984
                     /locus_tag="P5310_04440"
     CDS             2121..2984
                     /locus_tag="P5310_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRU32641.1"
                     /translation="MNEKLAGLLAVVLACIFWGTSFSVSKMLLTQVSPFELAFTRFLL
                     ASIFSFFLFFPKVRTAPLKWEYQKHLFMAGFLGVTLYFIFENWGLKFTSASEGALLVG
                     SFPALSLMLEMFKDKKSPSLNRVLGIALSLLGVTFIVGNAGIKLNIDNLFGDFLILLS
                     GLSWILYNRQIKRVNHIYPYEVLTTFQMIYGTILFIPLLSFTGLSFPKGTEGILGVLY
                     LAFFCSALGYLLYNYGLRRLELTQVVNTLNLIPLFGAIWGVIFLRETLGVWEILGGVL
                     IILGVSLTTWR"
     gene            3053..>3373
                     /locus_tag="P5310_04445"
     CDS             3053..>3373
                     /locus_tag="P5310_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32642.1"
                     /translation="MPKGIKFLSVFITLCIILSTGASGFAQKKTIRMDARDYYPRERL
                     STDRWNPPTYLWKLEKEYERLHPDIDIQFIRPPEGMDADVWLTTQLTGGTAPEISEQL
                     FSEVN"
BASE COUNT         1119 a          563 c          743 g          948 t
ORIGIN      
        1 aagagctaca agatatggtg gaacaatagt acctacctct ttctatcacg gtgaggcaga
       61 agagttgaat cttggagaag aatggcatct taaccgtcag gttatggttt caggagccag
      121 ggtagaaagt gaaccctata gagattatcc ccgttgggat aggacaagag tttataatac
      181 agtcatagag ctttttcttc tgaaaaagct ctccgttgaa ggattcttac atattgtaga
      241 ttttgaagat gtactggaag cttaccaaac actggagaaa gaaccaaata agtggataaa
      301 gctgggagta agatatggga gataggtaat aaagccatat cccttattct atccaattta
      361 actcttgcca gactgtgtcc attcttcagc gattttgaga tattaccctt cgtttattta
      421 agaattatat tctctatccc ttaccaacca aaagtgcaac ggtcactatg agtatgttat
      481 aatatccaaa taaaacagaa gggaatttag tatatgtttc acgtgaaaca ttggagagtt
      541 gtatgggaaa gataatcgct gttgcgaacc agaagggtgg agtagggaaa accaccacag
      601 ttataaattt gggatttgcc ctatccgaac ttggtaagag ggtccttctg gtagatggag
      661 accctcaggg aaattccacc agcggtataa ttcagaagag gaatataaaa ccaaatctat
      721 atgatgcgct gatctgtgat gtctctgtaa aagaggtgat actccccctt aaagatggaa
      781 agacttctat aagagataat atcagtctga taccttcaaa cctagaactt gcaggggcag
      841 agattgaact tgtgccggta tttttaagag agttaagatt aaaaaaagta ctggaaccag
      901 taaaagatga ctatgactat atctttattg actgtccccc atctttagga cttcttaccg
      961 ttaatgcgct atctgcatca caatacgttt taattcctct ccagtgcgag tattatgcac
     1021 tggaaggtat atctcaatta ctgaagacta ttgagcttat taagaaagac ttgaacccag
     1081 cattagagat tcttggtatt ctactgacca tgtataatag aacgatcctg gctcagcagg
     1141 tattggatga aacgaagaga ttctttaagg ataaagtctt taatacccta atcccaagga
     1201 atgtaagact aagtgaagct ccaggttttt ccctctctat ctttgaatat gcgcctgatt
     1261 cttcaggggc tggtgcgtat agagagctgg caagggagtt gatgaatatt ggaaaataaa
     1321 aggttaggca gaggtctctc agcgctaatc cctagcgaag aaaagaaaaa aggggtggaa
     1381 gagatcgaag tcgacaggat agtccctaat cctaaccagc ccagggaatc tataaacgaa
     1441 gagaacatta gagaattggc agaatcaata agagaaaaag gtatcttaca acccatactg
     1501 gttagaagaa aggatgatag atattacgag gttgttgctg gagaaaggag atatctttct
     1561 gcaaaattat tagggctgaa tagagtacct gccataatct tagatattga tgagaaagag
     1621 gcatatgaaa tatctctggt agagaacata cagagagagg acctaaatcc aatagaaaaa
     1681 gcagaggctt tcaaagacta tatagataaa tacagagtaa cacacgaaga attggcaggg
     1741 aaaatcggta taagcaggac agaaataacc aatctgttaa gacttcttca acttccccag
     1801 gaaataaagg aggaggtgaa gaaagggact cttacatatg gacatgctag gacactctta
     1861 agtttagaaa gtattgattc ccagaaagaa atagcagaaa agatcatcaa tgagaggcta
     1921 tctgtgagag aaaccgaaga actggtaagc aaaaagagag agattagaga gataccagag
     1981 ataagagctc ttgaaagtaa gctccaaaat tatttagaga ccagagtaaa aatacagcca
     2041 cagtctcctg agaggggaag aataactata gagtacaaga gcttagaaga attagagagg
     2101 atcataggga aatttctgga atgaatgaaa aacttgctgg attacttgct gttgtactgg
     2161 catgtatttt ctggggtacc tccttttcag tgtcaaagat gttattaaca caggtctctc
     2221 catttgaact ggccttcaca agatttttgt tagcatctat attctctttc tttttatttt
     2281 tcccaaaggt aaggacagct cccctaaaat gggaatatca gaaacattta tttatggctg
     2341 gatttttagg tgtcaccctg tattttatct ttgaaaactg gggactaaaa tttacttctg
     2401 catcagaagg agcattgctt gtaggttctt tcccagcgtt gagtcttatg ttagagatgt
     2461 ttaaagacaa gaaaagtccc tcattaaata gggtattggg gatagcgcta tctcttcttg
     2521 gtgtaacttt tatcgtagga aacgcaggta taaaattaaa tatagacaat ttatttggag
     2581 attttctgat acttctttct ggactgtcct ggatactgta taatcggcag ataaagaggg
     2641 ttaatcatat atatccgtat gaggtactta ccactttcca gatgatatat ggaacaatac
     2701 tctttattcc ccttttatcc tttacaggac tgagttttcc caaagggaca gaaggtatcc
     2761 tgggcgtact ttatttagca ttcttctgtt cagcactggg atatttgctc tataattatg
     2821 gacttagaag attggaatta actcaagtag tcaatacgct caacctgatt ccacttttcg
     2881 gcgctatatg gggtgtgata ttcttaagag aaacgctggg agtctgggaa attctaggag
     2941 gagtactcat tatcctgggg gtcagtttaa ctacctggag gtagaaggat ttgggattta
     3001 ttcagattaa cctggataca gaattaaaat taaaaagagg aggtatcgta aaatgcctaa
     3061 aggtataaaa tttctctctg tttttatcac cctgtgcatt atattaagca caggtgcgtc
     3121 tggatttgca cagaaaaaga ctatcaggat ggatgccagg gattattatc ccagagaaag
     3181 actcagcact gacaggtgga atcctccaac ttatctctgg aaattagaga aagagtacga
     3241 gagactgcat ccagatatag atatacagtt tataagacca cctgaaggta tggatgcaga
     3301 tgtatggtta accacccagc tgacaggagg tacagctcca gagatatcag aacaattatt
     3361 ctctgaggtc aat
//
LOCUS       DAOTKU010000145         3373 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_23396_length_3373_cov_3.169379, whole genome shotgun sequence.
ACCESSION   DAOTKU010000145 DAOTKU010000000
VERSION     DAOTKU010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23396_length_3373_cov_3.169379"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /locus_tag="P5310_04450"
     CDS             <1..712
                     /locus_tag="P5310_04450"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRU32643.1"
                     /translation="EGAEVLGVLEKLPSPGGPTRSVVQCLEDYGIPLHLNHTISEIKG
                     KYRVEGVVAEDILNNKKFCMDVDTILLSVGLIPENEISKKIGVQLDPATGGPVVDQDR
                     ETNVSGVFACGNVLLVHDLVDEVTREAYIAAEGAISYTSGKKRRERLIQLKPGENVRY
                     VVPQKISLPLEKPPYIYFRPREPLVNCQILGRADGKESLLGTTEYARPGEMIKLRLKR
                     DLLNSNLDIEISLTGEKE"
     gene            709..1056
                     /locus_tag="P5310_04455"
     CDS             709..1056
                     /locus_tag="P5310_04455"
                     /inference="COORDINATES: protein motif:HMM:NF019507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1667 domain-containing protein"
                     /protein_id="HRU32644.1"
                     /translation="MKILTCIVCPVGCKIKIDEDGNIQGARCDRGVGFVKRELLSSER
                     ILTTTVKIEGGIVRVIPVKSSRPVSKNKLRDIVKRISNISVPAPINRGDVVYEDNDIK
                     LVATRSVPEREDV"
     gene            complement(1067..1759)
                     /locus_tag="P5310_04460"
     CDS             complement(1067..1759)
                     /locus_tag="P5310_04460"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HRU32645.1"
                     /translation="MQNEFASQFMKSTLIFIYLPLILVLLIFVIHNLIEKQKLVKLGI
                     SDIDKLDGKMFEKYLEVLFEKLGYKVERTRYIGDYGADLIIQKDNVKTVVQAKRYKGK
                     VGVRAIQEAVAAKGYYNCDKAMVITNSHYTHQAEELADRNGVELWNRKDLIRNLLRIK
                     DENEIKVGQKISEDIPDNSKNKCAICGVTVSDKVRQYCLSHPERFGGKIYCYNHQKEI
                     GISKDQNTDKDY"
     gene            complement(1843..>3373)
                     /gene="nuoF"
                     /locus_tag="P5310_04465"
     CDS             complement(1843..>3373)
                     /gene="nuoF"
                     /locus_tag="P5310_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HRU32646.1"
                     /translation="GLQEEIKVLETGSLGSSTDGVVMVVYPEGIYYRNVKPEDVPVIV
                     EEHLLKGRPVRRLMMPSVTPGVVTKETTKFDVSKQPRIVLKNVGIINPESIEEYIGVG
                     GYEALGKALLEMTPQDVIQEIKSSGLRGRGGAGFPTGLKWELAYKAKGSPKYIICNAD
                     EGEPGTFKDRLILEGDPHKTIEGMIIAGYAVGANRGYIYVRGEYELSIERLEKALSQA
                     REYGFLGENIFGTDFSFDIYIKKGAGAYICGEETALIESLEGKRGEPRFKPPYPTDAG
                     LWGKPTVINNVETLANVPSIIQNGASWFRSFGTEKSPGTKVYSILGHIVNAGLIEVPM
                     GVTLREIIYGYGGGMRSGKFKIAQIGGTAGALLGEDALDIPMDFDSLKPLNATLGSGA
                     ILIMDDSTCIVEVLKSVVAFFKRESCGKCTPCRAGVSKLYNMVNNIHGSGPEIVDKIL
                     EISTYMQQTSFCALGQSPIMPVSSAVKYFKDEIIEHTCGECRTGRCHFEKVPEELELV
                     V"
BASE COUNT          914 a          739 c          663 g         1057 t
ORIGIN      
        1 ggaaggagca gaagtgctgg gtgtccttga gaagctccca tctccaggag gacctaccag
       61 aagtgttgtt cagtgcctgg aggactatgg aatcccgctc catctgaatc ataccatatc
      121 agagataaaa gggaagtaca gggtagaggg tgtagtggca gaagatattc tcaataataa
      181 aaaattctgt atggatgtgg atacaatact tctctctgtt ggacttatac cggagaatga
      241 gatctccaaa aagattggtg ttcagttaga tccagcgacg ggaggtccgg tggtagacca
      301 ggacagagaa acaaatgtga gtggtgtatt tgcctgtggt aatgtactgc tggttcatga
      361 cctggtggat gaagttacta gagaggctta tattgcagct gagggtgcga taagttatac
      421 ctctggtaaa aagaggagag agaggttgat acaactgaaa cctggagaga atgtgaggta
      481 tgttgtgcct cagaagatat ctcttccatt ggaaaaacca ccatacatat acttcagacc
      541 cagggaaccg cttgtcaatt gtcagatact gggaagggct gatgggaagg agtctctact
      601 tgggactaca gagtatgcca gaccaggaga gatgataaag ctcagactga agagagattt
      661 gcttaatagt aatctggata tagagataag tttaacggga gaaaaagaat gaagatactt
      721 acatgtatcg tttgccctgt tgggtgcaag ataaagatag atgaagatgg aaatatacag
      781 ggggcgaggt gtgatagagg ggttggtttt gttaaaagag agcttttaag ttcagaaagg
      841 attctgacta ccacagtcaa gatagaaggg ggtattgtca gggttattcc tgtaaaatcc
      901 tcaagaccag tctctaaaaa taaactcaga gacatagtga agaggatttc aaatatatcg
      961 gtacctgctc ccattaatag gggggatgtt gtgtatgaag ataatgatat aaagcttgtg
     1021 gctacaagga gtgtcccgga gcgagaagat gtatgatttg taacctctaa taatctttgt
     1081 cagtattttg atcctttgag ataccaatct ctttttgatg gttataacaa taaattttgc
     1141 ctccaaatct ttccggatga ctgagacagt actgccggac tttatcgctt acagttacac
     1201 cacagatagc acacttattt ttagaattat ccggtatatc ttctgaaatt ttttgaccga
     1261 cttttatctc attttcatct ttaattctga gcagatttct gatcaaatcc tttctattcc
     1321 atagctctac cccatttcta tcggcaagtt cttcagcctg atgtgtgtag tgactattag
     1381 ttataaccat cgctttatcg cagttataat atccctttgc agcgacagct tcctgtatcg
     1441 ctcttactcc aaccttacct ttataccttt ttgcctgaac cacagttttg acattgtctt
     1501 tttgaataat gagatctgcc ccataatctc cgatatatct ggtcctctct actttataac
     1561 caagtttctc aaacaggact tccagatact tctcaaacat tttgccatcc agcttatcta
     1621 tatctgatat ccctaacttt accaatttct gtttctctat aagattgtgg atgacaaaga
     1681 taaggaggac aaggattaaa ggtaaatata taaatattag tgtggatttc ataaattgag
     1741 atgcaaactc attctgcata atactctgct cctcttagaa ataaataggg ctcgccagaa
     1801 gtactacttt agtagccttc catgcgagcc cctctcagtg tattaaacta ccagctcaag
     1861 ttcttcaggt actttttcaa aatggcatct gcctgttctg cattctccac aggtatgttc
     1921 tattatctca tctttaaaat acttgaccgc tgaagataca ggcataattg gagattgtcc
     1981 cagtgcacaa aaacttgtct gctgcatata tgttgaaatc tccagtattt tatctactat
     2041 ctctggtcca cttccgtgta tattgtttac catattgtaa agtttcgata ccccagccct
     2101 gcaaggtgtg cattttccgc aggattctct cttgaagaat gctactacag acttaaggac
     2161 ctcaactata caggtagaat catccattat gaggatagca ccagaaccca gtgtggcatt
     2221 tagaggttta agactgtcaa agtccatagg tatgtcaagg gcgtcttctc ctaataatgc
     2281 accagctgtt ccccctatct gggctatctt gaattttcct gatctcattc ctccgccata
     2341 cccgtagatt atttctctca atgtaacccc cataggtacc tctataagtc ctgcattgac
     2401 tatatgccca aggatggaat aaacctttgt ccctggagac ttctcagttc caaaacttct
     2461 gaaccagctg gcaccattct ggattatgct gggcacattg gcgagggtct ctacattatt
     2521 tattacggta ggttttcccc atagccctgc atctgttgga tatggtggct taaatcttgg
     2581 ttctcctctt ttcccctcaa gagattctat aagagcggtc tcttctccac atatataagc
     2641 tcctgccccc ttctttatat atatgtcaaa actgaagtca gttccaaata tattttcccc
     2701 caggaatccg tattctcttg cctgagacag agccttttcc aatctttcta ttgagagttc
     2761 atactcacct ctgacatata tatatcccct gtttgcacca acagcataac ctgctataat
     2821 catcccttca attgtcttat gggggtctcc ctcaaggata agtctatctt taaatgtccc
     2881 tggttcccct tcatccgcat tacagattat atatttaggg ctacctttgg ctttataagc
     2941 cagttcccat tttaaacctg tcgggaatcc tgctccacct ctaccccgca gaccggagct
     3001 ctttatctcc tgtattacat cctgtggtgt catctccagg agggctttac ccagtgcctc
     3061 atatcctcca acacctatat attcctctat tgattccggg tttataatcc caacattttt
     3121 caaaactatt ctgggctgct ttgaaacgtc aaactttgta gtctcttttg taaccacccc
     3181 tggtgttacc gatggcatca taagtctcct gactggtctt cctttaagca ggtgttcttc
     3241 aactattact ggaacatcct ctggtttaac gttcctgtag tatatacctt cagggtatac
     3301 taccattaca actccgtcgg tgctgctacc cagtgaacct gtttccagga cctttatctc
     3361 ctcctgcagt ccc
//
LOCUS       DAOTKU010000146         3367 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_23448_length_3367_cov_3.498490, whole genome shotgun sequence.
ACCESSION   DAOTKU010000146 DAOTKU010000000
VERSION     DAOTKU010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23448_length_3367_cov_3.498490"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            102..482
                     /locus_tag="P5310_04470"
     CDS             102..482
                     /locus_tag="P5310_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015536166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HRU32647.1"
                     /translation="MDNRDLIRVAKETRKPMKLREDFSSGDVGCAILSGSGIVYTGVC
                     IDLACGLGFCAEVSAIADMLKNGETRIIKLAVAFPEDKIGVPCGRCREMMIQIDKENM
                     DTKIILGEDKEITLKELLPLHWLD"
     gene            complement(517..1563)
                     /locus_tag="P5310_04475"
     CDS             complement(517..1563)
                     /locus_tag="P5310_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32648.1"
                     /translation="MMGLTKEEIKEFALSAGLDLIGVANIERFENAPKDMHPATIFPE
                     ARSVIVVGKRILRGGWRGIEEGTYWPSYTYFDYHGLLNTVFIPYPLYELACFIEDHGW
                     EAVPYYPGVPENKSVGGPLRKDGIPPDVNLAIRIAGIAAGLGEIGWSKVFLTKKFGPR
                     QRLHAIITDMPIEPDPLVEPGSICKRDMACVAGCPGAIPHIKENRSIKIQIEDKIYEW
                     GDIDLGKCTLVYHGGDPRVSPFIHKDIPGFNFDVTEQEMNEETAYKFCWTLSTAKWRV
                     TREFPSGYVIEGHALLQKWGEAGSYGVCASRGCMRSCFDYLEKAGIIEQTFESGRFIR
                     RPRWLLPSKVEKKR"
     gene            complement(1563..2372)
                     /locus_tag="P5310_04480"
     CDS             complement(1563..2372)
                     /locus_tag="P5310_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32649.1"
                     /translation="MLRTEDVKNFAREIRVDLIGIADIERFNDIPPEHNPLSIFPETR
                     SVIVIGRRIPRGALRGIEEGTQFANYSLYGRDWLNNRILAMSTFKVSEFLEDNGWEAV
                     PVPNLPPEVPPSGIPVREGQPAPNVMIDIEDAAIKAGLGEIGHCGFFLTPEFGPRQRF
                     QIILTDLRLEPDPLFNGKVCDYPSCQSFCPLNAITAAGVIDYKKCNICENGVFPNPYH
                     SSGKPDRLGAICARSCLDYLERHNLIKNTFQNPFRKRPSWKVVSERRIEEG"
     gene            complement(2408..>3367)
                     /locus_tag="P5310_04485"
     CDS             complement(2408..>3367)
                     /locus_tag="P5310_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRU32650.1"
                     /translation="LTNEKIKDFAKKSGADLVGIANIERFDKAPPELHPRTIFPETRS
                     VIVLGCRILRGSFRGIKEWTEWSSYWIFGYGAGIYGVLDEATDMTRKFIESYGYEAVD
                     VPGIATLPEAGPLRPPVSPDRYPPNISISFRLAAAAAGLGELGYSKVFLTPEFGPRQR
                     FAIILTDARLEPDPIFRGELCTRCMRCVRECPAGAISKDKTISVEIEGRIFEWADLDY
                     GKCKLTHWGFNRNASPFVKKDLPGLNLDISKQNMSWKEAYDFGWTLANRVKYISLVSK
                     GISEIEQSGRPGSICGARGCIQACFDQLEKTGVIPKFYTPFIV"
BASE COUNT          922 a          775 c          625 g         1045 t
ORIGIN      
        1 gccacctcct ttattatttt attgcttcta gtatatctca attgcttaat tttgtaaatg
       61 agatatacta gaatactatc atatttagga aggtggtatc tttggataat agagatttga
      121 taagggttgc aaaagaaact cgaaaaccta tgaaattgag agaggatttt tcttctggtg
      181 acgttggttg tgccatcctg tctggctctg gaatagttta taccggtgtc tgtattgacc
      241 ttgcctgtgg tttgggattt tgtgccgagg tttcagctat tgcagatatg ttaaagaatg
      301 gcgaaaccag aataattaaa ttggctgtag cctttcccga agataagata ggggtaccct
      361 gtggaagatg cagagagatg atgatacaga ttgataaaga gaatatggat acaaagataa
      421 tccttggtga agataaagag atcacattaa aagagttact accattacat tggctggatt
      481 agaagaaatt cggggcaggg tatcctgccc cgagaatcat ctctttttct ccactttgga
      541 tggtagaagc catcttggtc ttctaataaa tctgccgctc tcaaatgtct gttctataat
      601 cccagccttt tccaggtaat cgaaacagct tctcatacaa cccctagagg cacagactcc
      661 gtagctgcca gcttctcccc atttctgtaa taatgcgtgt ccttctataa cgtatccaga
      721 tggaaattct cttgtaacac gccacttcgc agtggaaagt gtccagcaga atttataggc
      781 tgtctcctca ttcatctcct gctctgtaac atcaaagtta aagccaggga tatctttatg
      841 gataaaagga gagacccttg ggtcccctcc atgatataca agtgtacatt ttccaagatc
      901 tatatctccc cattcgtaga tcttatcttc tatctgtatc tttatacttc tgttttcctt
      961 tatatgaggg attgccccag gacatccagc tacacaggcc atatctcttt tacagatact
     1021 tccaggttcg actagagggt ctggttctat aggcatatct gtaattatcg catgtaatct
     1081 ctgtcttggt ccaaactttt tagtcaaaaa gaccttggac cagccaatct cgcccagccc
     1141 agctgctatt ccagctattc ttattgcaag attaacatct ggagggattc catctttcct
     1201 taaaggtcct cctacagatt tattctccgg aacaccagga tagtatggaa cagcttccca
     1261 tccatggtct tctataaagc aagctagttc gtacagaggg tacggtataa atacagtatt
     1321 caaaagccca tgataatcga agtatgtata ggatggccag tatgttccct cttcgatccc
     1381 ccgccatcca cccctgagaa tcctctttcc aacaactatc actgatcttg cttcagggaa
     1441 tatggtagcg ggatgcatat ccttaggggc attttcaaac ctctctatat tggctacccc
     1501 aatcaaatct agccctgcag aaagagcaaa ttcctttatc tcttcttttg taagtcccat
     1561 cattaaccct cttcaatcct tcgctctgag actactttcc aggaaggtct ttttctaaaa
     1621 ggattctgga aggtgttttt tatcaggtta tgtctttcca gataatccag acaagatctg
     1681 gcacatattg cacctagacg gtcaggttta ccagaactgt gataaggatt tggaaataca
     1741 ccattctcac atatattgca cttcttataa tcaatcactc ctgcagctgt tattgcattt
     1801 aaggggcaaa agctttgaca tgaaggataa tcacatacct ttccgttaaa gagaggatca
     1861 ggttctaacc tgagatcggt aagaataatc tggaatctct gtctgggtcc aaattcaggt
     1921 gtcaggaaaa atccacaatg tccaatctct cctaagccag cttttattgc ggcatcttca
     1981 atgtctatca ttacattagg agccggctgt ccctctctta cagggattcc tgaaggagga
     2041 acttcagggg ggagattggg tactggtaca gcttcccacc cattatcctc cagaaattca
     2101 gacactttaa aggtgctcat tgccaggatt ctattattta gccagtctcg tccatagagg
     2161 gaataatttg caaactgagt tccctcctct attcccctta atgctcctct cggtatcctt
     2221 ctccctatca ctattaccga ccttgtttca gggaatattg aaagtggatt gtgctctggg
     2281 ggaatgtcat taaacctctc tatatcagct atccctataa gatctactct tatctctctt
     2341 gcaaaattct ttacatcttc agtcctaagc atttaatttc ctcctatctt aaaactttag
     2401 ttaagaatca tactataaaa ggggtataga atttcggtat aaccccagtt ttttctagct
     2461 gatcaaaaca agcctggata catcctcttg ctccgcatat actgccagga cgcccagatt
     2521 gctctatctc actaataccc tttgatacta aagatatata tttgactcta ttagccaggg
     2581 tccaaccaaa gtcataagcc tctttccagc tcatattttg tttagatatg tcaagattta
     2641 atccaggtag gtccttcttt acaaaaggag aggcatttct attaaatccc caatgggtta
     2701 acttgcattt cccatagtct aaatctgccc actcaaatat cctgccctca atctctactg
     2761 agatggtctt atccttactg atagcaccgg caggacattc tctcacacaa cgcatacatc
     2821 ttgtgcatag ctcacctcta aatattgggt caggttctag tcttgcatcg gttagaataa
     2881 ttgcgaatct ctgtctggga ccaaactctg gtgttaaaaa gacctttgag taccctagct
     2941 ctcccagtcc agcggcagct gctgctagtc tgaatgagat agaaatattc ggtggatatc
     3001 tatctggaga tacaggaggt cttaagggtc cagcctcagg gagggtagct atccctggaa
     3061 catctacagc ttcgtaacca taggactcga taaacttcct ggtcatgtct gtagcctcat
     3121 caagtacccc atagataccg gcgccatagc caaatatcca atatgaagac cattctgtcc
     3181 actcctttat acctctaaaa gagcctctta agatcctgca tcctaataca ataacagacc
     3241 tggtttcagg gaaaatagta cggggatgta actccggtgg agccttatcg aatctttcta
     3301 tattggctat cccaactaaa tctgccccag atttcttggc aaaatctttt atcttttcat
     3361 ttgttaa
//
LOCUS       DAOTKU010000147         3351 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_23590_length_3351_cov_2.713289, whole genome shotgun sequence.
ACCESSION   DAOTKU010000147 DAOTKU010000000
VERSION     DAOTKU010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3351
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23590_length_3351_cov_2.713289"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="P5310_04490"
     CDS             complement(<1..596)
                     /locus_tag="P5310_04490"
                     /inference="COORDINATES: protein motif:HMM:NF012449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HRU32651.1"
                     /translation="MRKTKIVATIGPASDSPEIIKYFIQAGVEVFRLNFSHGDQGYHA
                     NIFNLIREISSSLKRPVAILQDLQGPKLRVGTLAQDSVILQQGEEVILKPGETSDDKN
                     IIPIKERELIEALRPGASIMLSDGLIELKVTEKRENGVACRVVNGGELKSKRGINVPN
                     ITINIPSVTSKDIEDLMFGIKLGVDFIAVSFVRKAADIL"
     gene            complement(593..1870)
                     /gene="eno"
                     /locus_tag="P5310_04495"
     CDS             complement(593..1870)
                     /gene="eno"
                     /locus_tag="P5310_04495"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HRU32652.1"
                     /translation="MSIITEIRGREILDSRGNPTVEVEVYLESGYVGRASVPSGASTG
                     VHEAVELRDGDASRYLGRGVKNAVKNVDEIIAPELIGRDAINQKSIDKILIELDGTPN
                     KGRLGANAILAVSLAVAKAAANFLEIPLYRYIGGTNTCTLPVPLMNIINGGKHADNPL
                     DFQEFMIVPAGAPSFREALRMGVETFHTLRKVLQNRGLNTNVGDEGGFAPNLSTNESA
                     IEAIIEAIQKAGYTPGKDIFVALDVAASNIYEDGKYRFGGKDYSVDELISYYETLLEK
                     YPIISIEDGLAEDDWDGWKIFTQRLGTKVQLVGDDLFVTNIKRLAKGIEMGVANSILI
                     KVNQIGTLSETSEAVEMAGRSGYTAVISHRSGETEDTTISDLAVATGCGQIKTGAPSR
                     TDRVAKYNQLLRIEEDLGPVATYLGVKAFSSRI"
     gene            2360..2533
                     /locus_tag="P5310_04500"
     CDS             2360..2533
                     /locus_tag="P5310_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32653.1"
                     /translation="MLPLLDRRSKRADWIRGEHIDFPDEIERLPKSLHWLFDNPEDFG
                     QVNKNTTVTLDTR"
     gene            complement(2680..3282)
                     /locus_tag="P5310_04505"
     CDS             complement(2680..3282)
                     /locus_tag="P5310_04505"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRU32654.1"
                     /translation="MENTWLDIYDAPEKLESLIDILVEMNLIAIEKYKVSGADGFIIA
                     DDWGLQNKLMISPSAWRKIWEPRYARIFEAAHNAGMFTFLHSCGYIVDILDDLIEIGL
                     DVIHMDQQENMGLELLGRRFGGRITFFSPVDIQNTMVYGTLDEIRSYCRNMVRTLARL
                     PNGGFIPRWYTDPEGAGHRQEAIEAMCEEFLKISKEIYGF"
BASE COUNT          901 a          807 c          587 g         1056 t
ORIGIN      
        1 agtatgtctg ccgcctttct cacaaatgat accgctataa aatctacccc taacttgatg
       61 ccaaacatca ggtcttctat atctttagag gtgaccgatg gaatattgat ggtaatattg
      121 gggacattta ttcccctctt gctctttaat tcgcctccat ttactactct gcatgccacc
      181 ccgttttctc ttttctctgt gacctttagc tcaataagcc catctgatag cataatagat
      241 gcccctggac gaagtgcctc tattaattcc ctctctttta tcgggataat atttttatca
      301 tcacttgttt ctccaggttt aagtatgact tcttctcctt gctggaggat aacactatcc
      361 tgagctaaag tgccgaccct aagtttgggt ccttgtaaat cctgaagaat agctaccggt
      421 ctttttagag aactggatat ctctcttata aggttaaaga tattggcatg atatccctgg
      481 tctccatgag aaaagtttag cctgaatacc tccacccccg cctgaatgaa atatttaata
      541 atctctggag aatcacttgc cggaccaatt gtggcaacta tctttgtctt tctcatattc
      601 tgctggagaa tgccttaacc cctagataag tggctacagg acctagatcc tcttctatcc
      661 ttaacagttg attatactta gccaccctgt ctgttctaga gggggcacct gtttttatct
      721 gtccacatcc tgtagctaca gccagatctg agatggtagt atcttcagtt tctccagacc
      781 gatgtgagat gacagctgtg taaccagatc ttcccgccat ttctaccgcc tctgaagttt
      841 cactgagagt ccctatctga tttaccttaa taaggatgga gtttgctacc cccatctcta
      901 ttcctttagc cagacgcttt atattggtaa caaacagatc atcacccaca agctgaacct
      961 ttgttcccag tctctgggtg aaaatcttcc agccatccca gtcatcttca gccagcccat
     1021 cttctatgga gatgattgga tatttttcaa gcagggtctc gtagtaagat ataagctcat
     1081 ctactgaata atctttccct ccgaagcgat acttcccatc ctcgtatata ttgctggcag
     1141 caacgtctaa cgccacaaat atatcttttc caggggtata tcctgccttc tgtatggctt
     1201 caattattgc ctctatcgca gactcatttg ttgatagatt tggtgcaaat cctccttcat
     1261 cccctacgtt ggtattgagc cctctgtttt gaaggacttt cctcagcgta tggaaggttt
     1321 ccactcccat tctcagggct tctctaaaag atggtgcacc agctggtact atcataaact
     1381 cctgaaaatc tagtgggtta tcagcatgct tccccccatt aatgatgttc ataaggggta
     1441 ctggaagtgt acatgtattt gttccaccga tatatctata gagaggtatc tccagaaaat
     1501 tagctgccgc tttagccacc gctagagata ccgccagaat agcatttgca cccaatctgc
     1561 ccttatttgg tgttccatct agctctataa gaatcttatc tatactcttt tgatttatag
     1621 catcccttcc tatgagctca ggggctatta tctcatctac attctttacc gcatttttta
     1681 cccctcttcc gagatatcta ctggcatctc catctcttag ctcaacagcc tcatgaactc
     1741 cggtggatgc cccggaagga acactggctc ttccaacata tccactttcc agataaactt
     1801 caacctcaac ggttggattc ccccgtgaat ccaggatttc tcttcctctt atttcagtga
     1861 tgatactcat tattttcttc ctccatttta ttttgttaac tgtgtggatg gttaaatttt
     1921 aattaattcc ttagctattg tcaagagtat gtgttaacgt tctgtgatat tgtcataggt
     1981 aaatagttct gacctatctc cctcccccca cctctatcct cccccgagat gggggcggaa
     2041 gaaaagagaa cgttcccctg atggactccc ctatttccct ctcccttgac gggagagggt
     2101 aggggtgagg gtgagaagta agagcgttcc cccgagatgg gggagggagg ataaaggcat
     2161 ggtgttttta ttataaaaga cgaaattgta ggggcaattc atgaattgcc cctacgggat
     2221 acaactcata tgtttcattt ccttcccctg acattttcac agactattga cagttaatct
     2281 atggttagta gacccattgt ttctgatgct agtcccataa tcatttttgc acgttccaat
     2341 catctatctg ttttgaaaga tgttaccgct tttagataga agaagtaaaa gggcagattg
     2401 gattagaggg gaacatatag atttcccgga cgaaatagaa agactaccca aaagcttaca
     2461 ctggcttttc gacaatcctg aagattttgg gcaggtaaat aaaaatacaa cagttacctt
     2521 agatacgaga tgacttccgt ccagtctgct gccggaaaag tactggataa tagcgtcatt
     2581 cagcaataaa tcaaaaataa gtattcactc tggtctattc ctttatatac tatcctgctg
     2641 catctacctg taaaacgatg atgaaaacct cttttctggt taaaacccat atatttcctt
     2701 actaattttt aaaaattcct cacacatagc ctctatagcc tcttgtctat gtcctgcacc
     2761 ttctggatct gtataccacc tggggataaa acctccattg ggtagacgag ccagggttct
     2821 taccatattt ctacaataag accttatttc atcaagagta ccatatacca tggtattttg
     2881 aatatcaaca ggagagaaga acgttatcct gccaccaaag cgtctaccta aaagttccag
     2941 ccccatattt tcttgctggt ccatatgaat aacatccagt cctatctcta tcagatcatc
     3001 cagaatatct acaatatagc cacaactatg cagaaaagta aacataccag cattatgtgc
     3061 agcttcaaaa atacgagcat aacgaggttc ccagatttta cgccatgcac taggagagat
     3121 cattaatttg ttctgtaatc cccaatcatc ggctataata aatccatcag ctcctgagac
     3181 cttatatttc tcaattgcaa taagattcat ctctaccaag atatctataa gagactccaa
     3241 tttttctggc gcatcatata tatctaacca ggtgttttcc aaaccgcgga taaaatgtac
     3301 cctctcatag atggaaatac cagaagctaa taagaattta tctccagcct t
//
LOCUS       DAOTKU010000148         3308 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_23979_length_3308_cov_3.639717, whole genome shotgun sequence.
ACCESSION   DAOTKU010000148 DAOTKU010000000
VERSION     DAOTKU010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3308
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23979_length_3308_cov_3.639717"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            47..1318
                     /gene="der"
                     /locus_tag="P5310_04510"
     CDS             47..1318
                     /gene="der"
                     /locus_tag="P5310_04510"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HRU32655.1"
                     /translation="MLPIIAIVGKPNVGKSTLFNRILGVRRAVTSPIPHTTRDRLYEV
                     SNWNGVSFTLVDTGGFTVKSDDPFQDEINFELKKTLEEAEKILFVVDAKSLITSDDLE
                     LADIIRNYGRKVILVVNKVDNFQQKDIILSDFYSLGFEEVIPISAYHGLNVDELLDKI
                     VDSLPSGSTEKTELLRVVLAGNVNVGKSSLFNKLIGKDRAIVDEIAGTTRDIIEEELD
                     LNGEKVFITDTAGIRRRWKEGSIVEKIAVSNTLRVINRGDLVLFVLDIGEGLTGFDKR
                     IAQSIIEMEKPVIVVWNKMDLLEKSGIRLPSKFPLIPYAPVCYTSSLTGRGIKRLKET
                     ISSTISAGRVEIKKSQLDSALSGLTLPGEDGKIIKVYYGKQIESLPPKFTLFVNSTKG
                     INERTQQEVIKRIRSIYPFTGNPIKIEWRKS"
     gene            1315..1908
                     /gene="plsY"
                     /locus_tag="P5310_04515"
     CDS             1315..1908
                     /gene="plsY"
                     /locus_tag="P5310_04515"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF014690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HRU32656.1"
                     /translation="MIVFLLLLFSFILGSIPFGYLISKYFYNIDIRSKGSGNIGATNV
                     LRTLGILPGITVLVTDMLKGAIPVIIGRSAGMPQEIFLLAGAMSILGHCFSPFLKFKG
                     GKGVSSTFGMILAFDPVVAILSFLVEAGVVLIWNYVSLGSIAAVISMVIFLIIRRHSV
                     ISIIIMILVATLISVRHRDNIKRLLEGKENRFTLKVH"
     gene            1910..2671
                     /locus_tag="P5310_04520"
     CDS             1910..2671
                     /locus_tag="P5310_04520"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HRU32657.1"
                     /translation="MPIPKALTSRINSLKSGIGEAADKYFLGEGWKVVKEGIEAGWLP
                     RWLILSGESLERYKEIFPLISSSVEIYVINSKEMEKLSTLETPPGIMGIFPRKDFAFP
                     SGDIIVLIDGVQDPGNLGTIIRTADAVNASSVIVLKNSVDPYNHKVVRGSMGSIFHIP
                     VFKWDRVEDLLTRIEKRYYIVGTSSHSGDLFYNFKWRFPLAVIFGNEGKGLSEEIEKI
                     CDATVKIPILGKAESLNVAVACGVILYEIIRNMIK"
     gene            2705..2932
                     /locus_tag="P5310_04525"
     CDS             2705..2932
                     /locus_tag="P5310_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32658.1"
                     /translation="MAIKVRYISREAKDLPDLYSVIPNDHPYVFDSPSQARQKAKSLT
                     LETGESYIVVKHLGYMEVLPETKKRGRKPKE"
     gene            3060..>3308
                     /locus_tag="P5310_04530"
     CDS             3060..>3308
                     /locus_tag="P5310_04530"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HRU32659.1"
                     /translation="MHGGIVTTLLDEAMAYALEYKNYRGVTARLHVKFIKPLRPEERY
                     RISGYLDRVRGKVAKTHSEILDEMGNTIAKASGIFILND"
BASE COUNT         1079 a          543 c          755 g          931 t
ORIGIN      
        1 ccccggagtg ggttatagaa aatgtgctgg agaagattaa agaattatgc tcccaataat
       61 agctattgtt ggcaaaccca atgttggtaa gtcgacactc ttcaacagaa tattaggggt
      121 aagaagagcc gtaacaagcc ccataccaca tactacaagg gatagattgt acgaagtcag
      181 caattggaat ggagtctcat ttacccttgt agatacaggt ggctttacgg taaaatctga
      241 tgacccattt caggatgaga taaactttga gcttaagaaa actctggaag aggcagaaaa
      301 gatactcttc gttgtggatg ccaagagttt aataaccagt gatgacctgg aacttgcaga
      361 tataataaga aactatggca gaaaggtgat actagtagta aataaagtgg ataacttcca
      421 gcaaaaagat attatactat ctgatttcta ttcacttgga tttgaggagg ttatacccat
      481 ttctgcctat catgggctaa atgtcgatga actcctggat aagattgtag attcccttcc
      541 ctctggtagt acagaaaaga cggaactgtt aagagtagta ctggctggca atgtcaatgt
      601 aggaaaatcc tccctattca ataaacttat aggtaaagat agagcaatag tggacgaaat
      661 agctggaaca accagggata tcatagaaga ggaactggat ttgaatggag agaaggtttt
      721 tattactgat acggctggta taaggagaag atggaaagag ggatctatag tagaaaaaat
      781 agctgtctcc aatactctga gggttataaa tagaggagat ctggtattgt ttgtactgga
      841 tataggcgaa ggattaacag gtttcgataa aagaattgct cagagcataa ttgagatgga
      901 aaaaccagta atcgttgtct ggaacaagat ggacctgctg gaaaagagtg gaattcgtct
      961 tccatccaaa tttccgctaa tcccatatgc ccctgtctgt tacacctcct ctctcacagg
     1021 aagaggtata aagagattaa aagaaacaat ctcttctact atctctgctg gaagagtgga
     1081 aatcaaaaag agccagttag acagtgcttt atctggtttg actcttcctg gggaggatgg
     1141 aaagataata aaagtatact atggcaaaca gatagaatct ctcccaccaa aattcacatt
     1201 atttgttaac agtacaaaag gtataaatga gaggactcaa caggaggtaa taaagagaat
     1261 aagaagtatt tatccattta ctggaaatcc cataaaaata gagtggagga aaagttgata
     1321 gtttttctat tacttctatt cagttttata cttggcagta tacctttcgg ttatctcata
     1381 agcaaatact tctataatat agatataaga agtaaaggaa gcggtaatat cggagcaacc
     1441 aatgtgctga gaacacttgg aatcttacca ggcataacag tgctggtaac cgatatgctt
     1501 aaaggagcta tcccggtgat cataggcagg tctgcgggaa tgccccagga aatctttctt
     1561 cttgctggag ctatgtctat tttaggtcac tgcttttcac catttttaaa attcaagggg
     1621 ggaaaagggg tcagttctac atttggtatg atacttgcct ttgacccggt tgtggctatt
     1681 ctttcttttc tggttgaggc tggagtcgta ctcatatgga actatgtctc tttaggctcg
     1741 atagcggcag taatatctat ggttatattc ctcattataa gaagacatag tgtaatatcc
     1801 ataatcataa tgatacttgt cgctacactt atctcagtcc gacatagaga taatataaaa
     1861 cgacttctgg agggtaaaga aaacagattt acactgaagg tccactagta tgccaatccc
     1921 aaaggctttg acttccagga taaactctct aaaatccggt ataggtgaag cagcggataa
     1981 gtattttctt ggtgaaggat ggaaggtggt taaagagggg atagaggcag gttggctacc
     2041 cagatggctc atcctcagtg gagaaagcct ggaaagatac aaagagatat ttcccctgat
     2101 atcctccagt gtggagattt atgttataaa ctcaaaagag atggaaaaac tctctactct
     2161 ggagaccccc ccaggtataa tggggatatt tccaagaaaa gactttgcct ttccttcagg
     2221 agatatcatt gttcttattg atggggtcca ggatccgggg aacctgggta ccattataag
     2281 aaccgctgat gcggtaaatg ccagtagtgt aattgtctta aaaaacagtg tggaccctta
     2341 caaccataaa gtggtacggg ggagtatggg gtctatattt catataccag tgtttaaatg
     2401 ggatagggtt gaggaccttc ttaccaggat agaaaagaga tattacatag tgggaacaag
     2461 ttcgcactct ggagacctgt tctataattt taagtggaga tttcccttag ccgtaatctt
     2521 cggtaatgag ggtaagggtt tatctgaaga gatagaaaaa atctgtgatg ctaccgttaa
     2581 aattcccata cttggaaagg cagaatctct taatgtagct gtagcctgtg gagttatact
     2641 gtatgaaata atacgtaata tgataaaata aagtgttaat tattttatca taggaggaat
     2701 ttgtatggct attaaggtgc gatatataag tagagaagct aaagacttac cagacttata
     2761 ctctgtaata ccaaatgacc atccttatgt gtttgatagc ccttcccagg ctagacagaa
     2821 ggctaaatca ttgacactag aaacaggaga gagttacatc gtagtgaagc atttgggtta
     2881 tatggaggtc cttcctgaaa ccaaaaaaag aggaagaaaa ccaaaggagt aatttcccat
     2941 actgttttgc ctgcggtaat ttaaatccta tagggcttca tctggagttt acagataagg
     3001 gacattatgt agaggcttta tttttacctg atatttttca tcagggctat ccagggataa
     3061 tacatggggg tatagttacc accctccttg acgaagctat ggcatatgca ctggaatata
     3121 aaaattatag aggggtaact gccaggcttc atgtgaagtt tataaagcca ttgagacctg
     3181 aagaaagata cagaatctca ggttatctgg atagggtgag gggcaaggtg gctaagactc
     3241 attcggaaat attagacgag atgggtaata ctatcgctaa ggcttctgga atatttattt
     3301 taaatgat
//
LOCUS       DAOTKU010000149         3280 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_24223_length_3280_cov_3.665116, whole genome shotgun sequence.
ACCESSION   DAOTKU010000149 DAOTKU010000000
VERSION     DAOTKU010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24223_length_3280_cov_3.665116"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..986)
                     /locus_tag="P5310_04535"
     CDS             complement(<1..986)
                     /locus_tag="P5310_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HRU32660.1"
                     /translation="MAKVIGIDLGTTNSVVAFVEGGKPTVIPNAEGSRLTPSAVAFTQ
                     DGQILVGELAKRQAILNPERTILSIKRHMGTNYKIRIDNKEYTPQEISAMILRKLKED
                     AEAYLGEKVEKAVITVPAYFNDAQRQATKEAGVIAGLDVMRIINEPTASSLAYGLDKE
                     GTQTILVYDLGGGTFDVSILEIGEGVFEVKATSGDTHLGGDDYDQRIINFLVEEFKRK
                     EGIDLSQDRMVLQRLKEAAEKAKIELSSLMATEINLPFITATNSGPKHMNITLTRAQF
                     EAMTKDLTERTREPIYRALSDAKLDPSRIDKVLLVGGSTRMPAVQRLVKEIF"
     gene            complement(990..1481)
                     /locus_tag="P5310_04540"
     CDS             complement(990..1481)
                     /locus_tag="P5310_04540"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HRU32661.1"
                     /translation="MEEELKENLPAEDIAMIKEQYKELEDKFLRLAADFDNYRKRMAR
                     EIEELKDVIRIELIRSLLPLLDDIERAFSSIPADRDDGIRDGIEMIMKNFQDWLTSQG
                     VKTIEISDDIFDPKYHEVVVVEEDENIDKDKVEELRTGYTLNDRVIRPTMVKIIKKVK
                     EGN"
     gene            complement(1485..2504)
                     /gene="hrcA"
                     /locus_tag="P5310_04545"
     CDS             complement(1485..2504)
                     /gene="hrcA"
                     /locus_tag="P5310_04545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HRU32662.1"
                     /translation="MGDDGSMLTEREKIILKAVVEEYINTGEPISSKVVMSKYCPYVS
                     SATVRNDMGVLEEMGYITQPHPSAGRIPSPKGYKYYVENLLDDCKLSPQQRMRIRKIL
                     SGLESEIRDLREMIRTTSWLIAQLSKLMAFSYGPISSGRHIKKLWLTPVNGESVLLVY
                     MTNVGEVNHKVISAKYNQYNLERLSNIFSSKLENEDMDRLDINEIVTLLPEEEPLIKE
                     VFEHLRREKYTTAESYIEGTDYIDKIPEFNNPEILKNLISIINKEDLVMEQINAAISS
                     GDVWTSIGEKGELTFIVVPYFMEDFPIGGAGVVGPVRMNYKYIIPLLNYLKNCISDML
                     SVRWR"
     gene            complement(2651..>3280)
                     /gene="hflX"
                     /locus_tag="P5310_04550"
     CDS             complement(2651..>3280)
                     /gene="hflX"
                     /locus_tag="P5310_04550"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HRU32663.1"
                     /translation="GPGQRKLETDRRHIERRIEFLGQEIERIKRHRTLIRSNRERNNV
                     PVIALVGYTNAGKSTLMNALTHSDVLVEDKLFATLDPTTRILWLDDIKVLLTDTVGFI
                     HKLPHHLVEAFSSTLEEIRYADIILNIIDASDPFFYEHIRVVDEMLKYLESYNKTIIR
                     VYNKIDLLQNQDDIIPVTEDTGVFVSAITGENLDKLKSLLKDQVKCISR"
BASE COUNT          924 a          767 c          484 g         1105 t
ORIGIN      
        1 ttgaatatct cttttaccaa tctttgaaca gccggcattc tggtagaacc accaaccagt
       61 aaaaccttat ctatcctgga agggtccagt ttagcatcag acaaagccct gtatataggc
      121 tctctagttc tttctgtaag gtccttagtc atagcctcaa attgagctct ggtaagagtg
      181 atattcatat gctttggtcc actattagta gcagttataa atggaagatt aatctctgta
      241 gccataagtg aagagagctc aatcttagcc ttttcagctg cctctttaag tctttgaagg
      301 accattctgt cctgactcag atcaattccc tctttcctct tgaattcctc aacaagaaag
      361 tttattatcc tctggtcgta atcatcacca cccagatgcg tgtcaccact ggtagcttta
      421 acttcaaata caccttctcc tatctccaga atagatacat caaaagtccc cccacctaaa
      481 tcataaacca gtatagtctg cgttccttct ttatccagac catatgccaa agaagacgcc
      541 gttggctcat tgataattct cataacgtca agtccagcta tgacccctgc ctctttcgta
      601 gcctgacgct gcgcatcgtt aaaatatgca gggacagtta taactgcctt ttctaccttt
      661 tctcccagat acgcctctgc atcctctttc aatttcctca gtatcattgc agagatctct
      721 tggggtgtat attccttatt atctatcctg atcttataat tagttcccat atgacgcttt
      781 atggaaagta tagttctttc aggattgagg atagcctgtc tcttagccag ctctcccaca
      841 agtatctgcc catcctgagt aaaagctaca gcagacgggg taagtctact accctctgca
      901 ttgggaataa ctgtaggctt tccgccctct acaaaagcta caactgagtt ggtagttcct
      961 aaatcaattc caataacctt agccatattt taatttccct cctttacctt cttaataatc
     1021 tttaccatag taggtcttat taccctgtca ttaagagtat aacctgttct taactcttca
     1081 accttgtcct tatctatatt ctcatcttct tcaactacta caacctcatg atatttagga
     1141 tcaaaaatat catcagaaat ctctatagtt tttactccct gactggtcaa ccaatcctgg
     1201 aaattcttca ttatcatctc tattccatct cttatcccat catccctgtc agcaggaata
     1261 gaagagaatg ccctctctat atcatctaat aggggaagca agcttctgat aagctctatt
     1321 ctgattacat cttttaattc ctctatctct cgagccattc tcttcctata gttatcaaaa
     1381 tctgctgcaa gcctcaagaa tttatcctct agttctttat attgctcttt tatcatcgct
     1441 atatcttcag caggaagatt ctccttcaac tcttcttcca tagttcacct ccaacggact
     1501 gaaagcatat cagatatgca gtttttcagg tagttcaaaa gtggaattat atatttgtaa
     1561 ttcatcctga ccggacctac cactccagct ccccctatgg gaaaatcctc cataaaatat
     1621 ggaactacta taaaggtgag ttcccccttc tctccaatac ttgtccatac gtctccggag
     1681 ctgatcgccg catttatctg ttccataact aaatcttcct tatttattat actgataaga
     1741 tttttaagaa tctctggatt attaaactct ggtatcttat caatataatc tgttccttct
     1801 atatatgatt cagcagtggt atacttttct cttctaaggt gttcaaagac ctcttttatt
     1861 aaaggctcct cttctggtag aagagttaca atctcattga tatcaagcct gtccatatct
     1921 tcattttcca gtttactgga gaatatattg gaaagcctct ccagattata ctggttatat
     1981 ttagcagaaa tgaccttatg attaacttca cctacattag tcatatatac aaggaggaca
     2041 ctctctccat tcactggtgt aagccagagc ttctttatgt gtcttccgga agaaattggt
     2101 ccatacgaaa aagccataag ttttgataac tgagctatca accaggatgt agtccttatc
     2161 atctctctca agtctcttat ctctgattct aaaccggata atatctttct tatcctcatc
     2221 ctctgctgag gagaaagttt acagtcgtcc agcagattct ctacataata tttataaccc
     2281 tttggactgg gtattctccc agccgaagga tggggttgag taatataacc catctcctct
     2341 aaaacaccca tatcatttct aacggtagca gaactgacat agggacaata tttagacatc
     2401 acaacctttg agctaatagg ctctccggtg tttatatact cttcaactac cgcctttaaa
     2461 attatctttt ctctctctgt aagcatactt ccatcatccc ccattagcac tccagagctt
     2521 taagtgctaa ttttatataa aaagtttatc atttactttg gtctttgtca agaaaaagct
     2581 tttattatcc cctgattaaa atcagaggta cttccagtgg aaattataga tttataagca
     2641 gaacacttaa ttatctagag atacatttaa cctgatcttt aagaagagac ttaagtttat
     2701 ccaagttttc tcctgtaatg gcagatacaa atacacctgt atcttctgtg accggtatta
     2761 tatcatcctg attttgtaag agatcaatct tattatagac tctgattata gtcttattat
     2821 aagactcaag atatttaagc atctcatcta ccacccttat atgctcgtag aaaaatgggt
     2881 cacttgcatc tataatattc agtataatat cagcgtatct tatctcttcc aaggtggaag
     2941 aaaaagcttc caccagatga tgcggaagtt tatgtataaa tccaacagta tcggtcaata
     3001 aaacctttat atcatcaagc cataatattc tggtggtagg gtccaaagta gcaaaaagct
     3061 tatcctctac cagcacatcg gaatgtgtaa gagcattcat taaggtggat ttacctgcat
     3121 tggtataacc aacaagtgcg atcacaggaa cattatttct ttctctattc gaccttataa
     3181 gcgtcctatg tctcttaatc ctttcaatct cctgccctaa aaattctatc ctgcgttcaa
     3241 tatgccgtcg atctgtttcc aattttcttt gtccaggtcc
//
LOCUS       DAOTKU010000150         3276 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_24258_length_3276_cov_3.862465, whole genome shotgun sequence.
ACCESSION   DAOTKU010000150 DAOTKU010000000
VERSION     DAOTKU010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3276
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24258_length_3276_cov_3.862465"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="P5310_04555"
     CDS             complement(<1..579)
                     /locus_tag="P5310_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HRU32664.1"
                     /translation="MEKNKLDIKALENWLWEAACKIRGPIDAPKYKDYILPLVFLKRL
                     SDVFDDEINRLSQKYGSRKIVEQLLAQDHNLVRFYLPEKTRWEEIAKQTTNVGEYLTD
                     AVRTISRENQKLQGVIDTVDFNATAAGQRIISDENLKGLIDVLSRYRLGLNDVDADIL
                     GRAYEYLLRKFAEGSGQSAGEFYTPGEVAILMS"
     gene            complement(842..1831)
                     /locus_tag="P5310_04560"
     CDS             complement(842..1831)
                     /locus_tag="P5310_04560"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HRU32665.1"
                     /translation="MNNNYTRQEIEDQPGVWENLINNPPRLEEILDKDNVIFFGSGTS
                     HYLALSAASFTQHYTGIVSRGLPSQEIAFFPEATMPDRDTLYVGISRSGETSEVVIAT
                     ETIKERGKGNIIGITTEIDKPLARLAERSIVIGEAKEKSVVMTKSFTSMLLAIQLGVL
                     STVNPSAIDTMKDEFSRSSRKILTIAEAWAKGEAKNNYKEIIFLGSGPLYGIACESAL
                     KVREMSASTATAFHSLEFRHGPKASLTKDSFVVMFISDSAREEELKIAKEIEDVGSKC
                     TIIGYDLPLSLSEFLRMILYMPFSQYLGYYKALEKGINPDTPINLTRVVRIEE"
     gene            complement(1846..2586)
                     /locus_tag="P5310_04565"
     CDS             complement(1846..2586)
                     /locus_tag="P5310_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011067811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HRU32666.1"
                     /translation="MSKIEEYFDEVEKILAKVRETQIKGIEEAANAIAEAVLKGNNIF
                     IFGASHSGMMAEELVYRAGGFMLINPIFAPGLSLSERPVNRTSKLERLEGFAKILLES
                     YPVKKGDVLIIASVSGRNPVPVEMALEAKDKGLTVITLTSLDYSKNVTSRHKSGKKVY
                     ELADIVLDDCCPKGDAVLQIGKFPQKVAPTSTIAGAYILNSMIARVVEILAERGIEPP
                     VFISGNLDGGDEYNSRKLAEYKDRIFYM"
     gene            complement(2598..>3276)
                     /locus_tag="P5310_04570"
     CDS             complement(2598..>3276)
                     /locus_tag="P5310_04570"
                     /inference="COORDINATES: protein motif:HMM:NF002805.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar isomerase domain-containing protein"
                     /protein_id="HRU32667.1"
                     /translation="STQSENIQEAASLIADGLMDGKAVYLYDHGGHSLQFEGIGRAGG
                     PMFLQPFTFNLNMNIPVHPLRKPKEVLEPYELADLILKYNNLEEGDTIIMGSVSGRNP
                     IPVELAIKARERGIRTVGITSITYSKAFPSRHPSGKRLFEVVDIAIDNCGVIGDAVLE
                     VEGIDVNICPTSGITGIFILWLLMAEVIEILLSKGVRPHIYKSINFDDGREYNEKALA
                     EYKKTGV"
BASE COUNT          849 a          783 c          550 g         1094 t
ORIGIN      
        1 agacatcaat atagcgactt ctccgggggt ataaaactct ccggcactct gtccggaacc
       61 ttcggcaaac tttcttaaga ggtattcgta agcccttccc aaaatgtcag catctacatc
      121 gtttaaacca agtctatatc tgctcaaaac atctatgagt cctttcaaat tctcatcgct
      181 gatgattctc tgccctgcag cagtggcatt aaaatctact gtgtctataa cgccctgaag
      241 tttttggttt tctcttgaaa tcgtgcgaac agcatcggtt aaatattctc cgacattggt
      301 ggtctgtttg gcaatttcct cccatcttgt cttttcaggt agataaaagc ggacgaggtt
      361 atggtcttgg gctaataact gttcaacaat cttcctggag ccgtactttt gggagagcct
      421 atttatttca tcatcgaata cgtcggaaag cctttttaaa aatacgaggg gcagaatata
      481 atccttatac ttaggtgcat caattggtcc tctaatttta caggcggctt cccatagcca
      541 gttttccaat gcctttatat ccagtttgtt tttctccaca ctactttaac ctccgtatca
      601 aaataatcaa ccaaatatat tataatcttt tccaggtact aaatgttctc tttatacgct
      661 gcagattatg atacaagata ctttccgaca caacagtctc aactatattg tgcttactcc
      721 cctgattctg caggaaggca tctgggcgaa cgaggtaagc cgtatacctg tagaaatacc
      781 ccttatctct aagattactt tgtagtgttc tatattcctt gaactgctct aaatctattc
      841 tctattcttc tatccttaca acacgcgtta agtttatggg agtatctgga tttatcccct
      901 tttccagagc cttatagtat cccagatatt gagaaaaagg catatagaga atcatcctca
      961 gaaactcact gagagatagt ggaaggtcat acccgatgat cgtgcattta cttcccacat
     1021 cttctatctc tttggctatc tttagctcct cttctctggc agaatcagag atgaacatta
     1081 ctacaaaaga gtcttttgtc agacttgcct taggtccatg cctaaattcg agtgaatgaa
     1141 atgcggtagc cgtagaggct gacatctctc tcaccttcag ggctgattca caggcaattc
     1201 catacaatgg acctgaacct aaaaagatga tctctttata attatttttt gcctctccct
     1261 ttgcccaggc ttcagcgata gtaagaatct ttcttgagct tctactgaat tcatctttca
     1321 ttgtatcaat agctgaaggg ttaactgtac taagtacacc cagttgaatg gctaacagca
     1381 ttgaggtaaa agatttcgtc atcaccacac tcttttcttt tgcctcccct atcactatcg
     1441 atctttcggc aagtctagcc agaggcttgt ctatctctgt agttatccct atgatattcc
     1501 cctttcctct ttcttttatc gtttcagttg caataactac ctctgaggtt tctcccgatc
     1561 tggatatacc aacataaagg gtatctctat ccggcattgt agcttctggg aagaatgcaa
     1621 tttcctgtga aggtaatcct ctggaaacta tcccggtata gtgctgtgta aaggatgcag
     1681 ctgacagggc aagatagtgc gaggtaccac taccgaaaaa tataacattg tctttatcta
     1741 aaatctcttc taacctggga ggattattta ttagattctc ccaaactccc ggctgatctt
     1801 caatttcctg tctggtataa ttgttattca ttctgtaata cctccttaca tatagaatat
     1861 cctgtcttta tactcagcta gtttcctgct gttatactcg tctcctccat ccaaattacc
     1921 acttataaat actggcggtt caatccctct ttctgcaaga atctctacca ccctggctat
     1981 catagaattg agtatatatg ctccagctat tgttgaagtt ggagccacct tctgagggaa
     2041 tttacctatc tgcaatacag catcaccttt agggcaacaa tcgtccagta ctatatcagc
     2101 caattcatat acctttttac cgctcttatg cctggaagtt acattctttg aataatccag
     2161 tgaggtaaga gttattactg taagcccctt atccttagcc tccaatgcca tttcaactgg
     2221 tacaggattt ctcccagata cagatgctat tatcaagaca tcacctttct ttaccgggta
     2281 tgattcgagc aggatctttg caaaaccttc tagtctttca agtttacttg tcctatttac
     2341 aggtctttca gatagggata accctggagc aaaaattgga tttataagca taaaaccccc
     2401 agccctgtat accagttcct ccgccatcat ccctgagtgg cttgctccaa agataaatat
     2461 attattccct tttaatacag cttctgctat tgcattggct gcctcttcta tgccttttat
     2521 ctgagtctct cttacctttg ccagaatctt ttctacttca tcaaaatatt cttctatctt
     2581 gctcatactt cctcctttta tacacctgtt tttttgtatt ctgcaagagc cttctcatta
     2641 tactctcttc catcgtcaaa gtttatgctc ttgtatatgt gaggtcttac tcctttagat
     2701 agtaatatct ctatcacctc tgccataaga agccacagta taaatatgcc ggtgattcca
     2761 ctggtaggac atatatttac atctatccct tctacctcaa ggacggcatc tccgataact
     2821 ccacagttat ctatcgctat atctactacc tcgaatagtc tcttaccact gggatgtctt
     2881 gagggaaagg cttttgaata agttatggag gttatcccta cagtcctgat acctctttct
     2941 cttgctttta tagccagttc taccggtatg ggatttctac cggagaccga ccccataata
     3001 atggtatctc cttcctccag gttgttatat ttaagtatta gatcagccaa ttcatagggt
     3061 tctaaaacct ctttcggttt acggagtgga tgtactggta tgttcatatt cagattgaat
     3121 gtgaatggct ggagaaacat aggtcctcca gctcttccaa taccttcaaa ctgcaacgaa
     3181 tgtcccccat ggtcatagag atacaccgcc tttccatcca tcaatccatc agcgatcaga
     3241 gatgctgcct cttgtatatt ctccgattgg gtactg
//
LOCUS       DAOTKU010000151         3267 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_24347_length_3267_cov_2.958593, whole genome shotgun sequence.
ACCESSION   DAOTKU010000151 DAOTKU010000000
VERSION     DAOTKU010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3267
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24347_length_3267_cov_2.958593"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /locus_tag="P5310_04575"
     CDS             complement(<1..557)
                     /locus_tag="P5310_04575"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HRU32668.1"
                     /translation="MSTVLTPADIESLGRELLKIAIRDDAIDLYRDSFRRLIDEVAYN
                     LELRAVWTNEDAGLKKKLILEHLSDRFPERLVEFVYKIIDLKAENFLQQIYLHFIELV
                     NTNQYSKVDIHLPFPIPEERLEEIDKILSEILSKEVICSVKIDSGIINGLKIDLEEEV
                     IDLSLKTQLDILKERLELIQARDSI"
     gene            complement(554..1048)
                     /gene="atpF"
                     /locus_tag="P5310_04580"
     CDS             complement(554..1048)
                     /gene="atpF"
                     /locus_tag="P5310_04580"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HRU32669.1"
                     /translation="MISFSFPTFIFETINFIITLYLIYRFIYRPLSSYITKRQESIQN
                     ELETARLEKEKASQLRAELEEKLQEAQKEAEAIINKAKEQAEVERAQILDRAQREIQA
                     LMTKTQNELLLERSHAIEKFKDEVTSIVVDVTSKLIMSELNNKDEVREKIFDNYLKEI
                     ETYR"
     gene            complement(1049..1315)
                     /gene="atpE"
                     /locus_tag="P5310_04585"
     CDS             complement(1049..1315)
                     /gene="atpE"
                     /locus_tag="P5310_04585"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005952243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HRU32670.1"
                     /translation="MLTGKALVIIASIISAGFGMGIAVLGGALGEGNAVSKAMESLGR
                     QPEAQSSIMTILLLGLAFIESLVIFMLVVALVLLFVNPFVGMVQ"
     gene            complement(1336..1992)
                     /gene="atpB"
                     /locus_tag="P5310_04590"
     CDS             complement(1336..1992)
                     /gene="atpB"
                     /locus_tag="P5310_04590"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HRU32671.1"
                     /translation="MEEVTPRVVFSILGIPVTDVVTTTWIVMAIIVFVGWILTRSLST
                     KTSKMQVLLESLIEFYENIAVQVMGEEGKKFVPLAISIGLFILFSNWIEVIPGFHAPT
                     RDLSTTIALAIVAVSSTYIAGIRKKGLHFFKKYIEPNPIFLPINILEELVRPVSLAFR
                     LFGNVLGEEIVIAILVMIVPLILPIPMILLGLFTGLIQAFIFSLLTLLYVAGAVGEET
                     "
     gene            complement(2333..2548)
                     /locus_tag="P5310_04595"
     CDS             complement(2333..2548)
                     /locus_tag="P5310_04595"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HRU32672.1"
                     /translation="MSSNPRGPFRVWQYLGIALSLGFTIVASIFIGLYIGGFLDRKLL
                     SGYSFRVLFMFLGAITGLRSVYRQFKK"
     gene            complement(2532..>3267)
                     /gene="wecB"
                     /locus_tag="P5310_04600"
     CDS             complement(2532..>3267)
                     /gene="wecB"
                     /locus_tag="P5310_04600"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HRU32673.1"
                     /translation="NPFPEEAYRRFISVISSLNLAPTRKAKENLIKEGIKEESILVTG
                     NTIVDAVYWISKDISDIISDKRIILVTAHRRENWGEPMRELVLAIKELTGMFSDIRFV
                     IPLHKNPLVRETFSSILSEEGRVDLIEPLDYPSLIRVMKSSFLILTDSGGIQEEAPTF
                     GKPVLVLRETTERPEGIEKGVAKLIGMRKENIVNNVKALLTDESLYLAMSKAGNPYGD
                     GNAGRRVIKAIMYFLGMGSKPDEFES"
BASE COUNT          989 a          714 c          510 g         1054 t
ORIGIN      
        1 tagatagaat ctctggcctg gataagttcc aatctttctt ttagaatgtc taactgtgtc
       61 tttagagaga ggtctattac ctcctcctcc aaatctatct ttaatccatt tattataccg
      121 ctgtctatct ttaccgaaca tataacttcc ttactaagga tctctgaaag gatcttatca
      181 atctcttcaa gcctctcttc agggatagga aatggcagat gaatatctac tttggagtac
      241 tgattggtat ttaccagttc tataaaatgg agatatatct gttgaagaaa attctcagcc
      301 ttgaggtcta taatcttata gacaaactca accagcctct ctggaaatct atctgagaga
      361 tgctctagga taagcttctt tttcaatccg gcatcttcat ttgtccaaac agctcttaat
      421 tctaaattat aggcaacttc atctataagt cttctgaatg aatccctgta taaatctata
      481 gcatcatctc ttatcgctat tttcagcagt tctctaccca gagactctat atcggctggg
      541 gttaataccg tactcatcta taggtttcta tctccttaag atagttgtca aatatttttt
      601 ctctgacctc atctttatta ttcaattcac tcattataag tttagaagtg acatcaacca
      661 ctatggatgt aacctcatct ttaaatttct ctatcgcatg gcttctctca aggagaagtt
      721 cattttgagt cttggtcatc agtgcctgaa tctctctctg agctctatct aaaatctgag
      781 ccctttctac ttcagcctgt tccttggctt tatttattat ggcttctgcc tccttctgag
      841 cctcctgtaa cttttcttca agttctgctc ttaattgaga agccttttcc ttttcaagtc
      901 tagctgtttc aagctcattc tgtatactct cctgtctctt agttatatat gaagatagtg
      961 gtctataaat aaatctatat atgaggtaaa gagtaattat aaagtttata gtctcaaata
     1021 taaaggttgg aaatgagaaa cttatcattt attgaaccat ccccacgaaa ggatttacaa
     1081 agagaagtac taaggcgacc accagcataa atatcaccaa ggattctatg aatgccagcc
     1141 ccaataaaag tattgtcata atagaacttt gagcttcagg ctgtcttccc agagattcca
     1201 tagccttgga tactgcattt ccctctccta aagctccacc caatacagct atccccatcc
     1261 cgaatccagc agatataata gatgctatta taaccagagc cttaccagtt aacatataat
     1321 attatcacct cctagttaag tttcttctcc cactgcaccg gccacatata atagtgtgag
     1381 gagagaaaat ataaatgcct gaataagccc ggtgaaaagt ccaagtaata tcattgggat
     1441 cggtaagatt aacgggacta tcattacaag tatagctatt actatctctt caccgaggac
     1501 atttccaaaa agacggaaag ccagagatac aggtcttacc agttcctcta atatatttat
     1561 aggtaagaag ataggatttg gttctatata ttttttgaag aaatggagac ctttcttcct
     1621 tatacctgct atataagtag acgaaacagc aactattgct agggctatcg tcgtactgag
     1681 gtctctggta ggagcatgga atcctggtat tacctctatc cagttagaaa aaaggataaa
     1741 taatcctatc gaaattgcca gtgggacaaa cttctttcct tcctctccca tcacctgcac
     1801 cgctatattc tcataaaatt ctataagact ttcgagtaac acctgcatct tgcttgtttt
     1861 tgtggacagg cttcttgtca ggatccagcc tacaaaaact ataatcgcca taactatcca
     1921 tgtagtggta acaacatctg tcaccggtat tccaaggata ctaaatacta ctctgggtgt
     1981 tacttcttcc atcttaaaat cctaattcct cctataaata gcactattgg atacactatt
     2041 ataccaaata tcaatccaac tatatttaac ctattaatta taccataaat agatagaagt
     2101 aataatatca gaataacaat aagaatcctc aatagcatat tccagataac ccctgtatag
     2161 gataaagatt ttcttttttt aaatgagata tagagaaggg agaaatcaac cagtccaatc
     2221 gttaacccaa ttagataccc tagaagatat cttgtattgt acagggataa gaaaaagccc
     2281 aagatcaaag ataagtagat atagattctc aagatataag agaatatttt attcacttct
     2341 taaattgcct gtaaacactc ctcagtccgg taatcgctcc gagaaacata aagagtactc
     2401 tgaaggaata tccactgagt aattttctat caaggaatcc tcctatatat aatccgataa
     2461 aaatagaggc tactatagtg aatccgagag aaagtgctat acccaaatac tgccataccc
     2521 tgaatggtcc tctaggattc gaactcatca ggtttacttc ccattcccag gaagtacatt
     2581 attgccttta taactcttct accggcattc ccatctccat aaggattacc cgccttactc
     2641 attgccaggt aaagtgattc gtctgtaagt aaagccttta cattattcac aatattctct
     2701 tttctcatcc ctataagttt tgccacacct ttttctatcc cctccggtct ctcggtagtc
     2761 tctctcagaa caaggaccgg tttaccgaac gttggagctt cttcctggat acctccggaa
     2821 tctgtaagta tgagaaatga gctcttcatt actctaatga gcgatggata atccaatggc
     2881 tctataagat ctactcttcc ctcttctgaa aggatacttg agaatgtttc tcttaccaga
     2941 ggattcttat ggaggggtat aacaaacctt atatcagaaa acataccagt gagttctttt
     3001 atagccagaa ccagctccct catcggttct ccccagtttt ctcttctatg ggcagttacc
     3061 agaataatcc tcttatcgga aataatatca gagatatctt ttgatatcca gtaaactgca
     3121 tccacaattg tatttccagt aaccagtata ctttcctctt ttatgccctc ttttataaga
     3181 ttttccttag ccttcctggt aggagctaaa ttaagactgg atattaccga gatgaatctc
     3241 ctgtaagcct cctcaggaaa ggggtta
//
LOCUS       DAOTKU010000152         3259 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_24411_length_3259_cov_3.095194, whole genome shotgun sequence.
ACCESSION   DAOTKU010000152 DAOTKU010000000
VERSION     DAOTKU010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3259
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24411_length_3259_cov_3.095194"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1145)
                     /gene="purD"
                     /locus_tag="P5310_04605"
     CDS             complement(<1..1145)
                     /gene="purD"
                     /locus_tag="P5310_04605"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HRU32674.1"
                     /translation="MKVLIVGSGGREDAIAYKVSQSPLLSELYCIPGNGGMAQRGKIL
                     EGKVEDVPEIAKDLGIEFVIVGPEAPLVDGIVDRCREYNISIFGPDSRGAQLEGSKIF
                     AKKFMKKNNIPTAEFYIPSDYNDAVRYIKENGLKAIKADGLAGGKGVVIPEDLDSASS
                     VIYRFMVERVLGKSGEMIVLEDILVGREISCIVITDGSNYCSLPIAQDYKRAYDGNRG
                     PNTGGMGSIAPIYIGEEIRNKIKDRIIERTLEGLRREGITYRGVIYLGLMLVDNDPYV
                     LEYNVRLGDPETQAIIPLLKDDILEIFYETATSNLSLSKLNLKPCYTCCVVLVSGGYP
                     GHYEVGKEITGLDDISECILFHAGTKLVDGRFYTAGGRVLNVVGSGET"
     gene            complement(1142..>3259)
                     /gene="polA"
                     /locus_tag="P5310_04610"
     CDS             complement(1142..>3259)
                     /gene="polA"
                     /locus_tag="P5310_04610"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HRU32675.1"
                     /translation="DKNVRVIVPRKGVTEVEVFDIGAVEEKFGISPHKIPLFKALAGD
                     SSDNIKGIEGIGKSSAVEILRDIDTVDELIANIQYLPNRIRVKLEGKEDEVRENLFLA
                     TLKRDVPVEIDLNDIAVRDPDLDKVKSIAQELGMKSILKRFSIETPDYNHNTGTAAEK
                     KGIKDILDIIDSSSYLSLFSDLDLSIYPVKLKSISISIEDKTYRLDEDEDVLKNFIRD
                     LFSERRTIAVYDMKSLWKVLGIYERELTSEILDVLILGYLIDPSQEVSLNKLSDRYLG
                     MNLPESCAIYKLGLILKEEIDKNDMNKLYQEIELPLTMVLADMELKGVLIDAEYLKEL
                     SKMLDLSLSQIEREIYNLAGEEFNINSPKQLSLILFEKLKLPPVKKTKTGYSTDAEVL
                     SQLASESEICARLLEYRELTKLKSTYVDALPKLINPHTGRLHTTFTQTGTSTGRLASS
                     DPNLQNIPVRSEIGKEIRRAFIAPKGYKLISADYSQIELRILAHLSGDPLLIDAFNKG
                     VDIHAQTAAKILGVEQEEVTPNMRRLAKTINFGIIYGMSDYGLSKELGIHRREAKEYI
                     ENYFNTYSGVKEFLEKLKEEAREKGYVTTIFNRRRYIPQIRSSNKAERELGERLAVNT
                     PIQGSAADLIKLAMVRLYDILRQKGLSASIILQIHDELLIETKEDMVEEVAHIVKDTM
                     ENVYKMKIPITVDVKIGDNWCEI"
BASE COUNT          921 a          757 c          484 g         1097 t
ORIGIN      
        1 gtctcacctg aaccaacaac atttaaaact ctgccccctg cagtataaaa tctaccatca
       61 accagcttgg ttccagcgtg aaataagata cactcagaaa tatcatctaa tcctgttatc
      121 tcttttccga cctcataatg tccaggataa cctcctgata ccagaacaac acaacaagta
      181 taacaaggtt tcaggttcag tttagacaga gataaattag aggtagcagt ctcgtaaaat
      241 atctctaaaa tatcatcttt caataaagga ataatagcct gggtctccgg atcaccaagc
      301 ctgacattat actctaacac gtaagggtca ttatctacca gcataagtcc caaatagata
      361 acacctctat atgttatccc ttctcttctt aaaccctcca gggttcgttc tattattcta
      421 tctttaatct tattccttat ctcttcccct atatatatgg gagctatact ccccatacca
      481 cctgtatttg gacccctgtt accgtcataa gctctcttat aatcctgtgc tatgggcagg
      541 gaacagtaat tacttccatc ggtaattaca atgcaggaga tctctcttcc aaccagtata
      601 tcctctaaaa ctatcatctc tccagactta cccagaactc tctcaaccat aaatctataa
      661 atgacactgc tggcagaatc taaatcctcc gggataacca caccttttcc accggcaagt
      721 ccatcagcct ttatcgcctt aagtccattc tcttttatat atctgaccgc atcattataa
      781 tcagagggta tgtaaaactc cgcggtgggg atattatttt ttttcataaa ttttttggcg
      841 aagatcttgc taccttcaag ttgagcccct ctggaatcgg gtccaaatat agagatatta
      901 tattccctac atctatccac tattccatct accaggggag cttcaggtcc tactattaca
      961 aactctattc ccaagtcttt ggctatttca ggaacatctt ctaccttccc ctctaaaatc
     1021 tttcccctct gtgccatacc tccattgcca ggaatacagt acaattcaga aagtaaaggg
     1081 ctttgagata ccttgtaagc tatagcatct tcccttccac cactaccaac tataagaact
     1141 ttcatatctc gcaccaatta tctcctatct ttacatctac tgttattggt atcttcatct
     1201 tataaacatt ctccatagta tctttaacta tgtgtgccac ttcctccacc atatcctctt
     1261 ttgtttcaat gaggagttca tcatgaatct gcaatattat gctggcagag agacctttct
     1321 gtcttaatat gtcatacagt ctgaccatgg ctaattttat cagatcagct gcactaccct
     1381 gtataggagt atttacagcc aatctttctc ccaactctcg ttcagcctta ttactgctcc
     1441 ttatctgggg tatatatctt cttctattga aaatagtggt tacataacct ttctctcttg
     1501 cctcttcctt taatttttcc aaaaattcct tgaccccact gtaagtattg aaatagttct
     1561 ctatatattc ttttgcctct ctcctatgta tgccaagctc tttcgaaaga ccataatcac
     1621 tcatcccgta tattatacca aaatttatgg ttttagctaa tcttctcata ttaggagtaa
     1681 cttcttcttg ttcaactcct aatattttgg cagccgtttg agcgtgaata tccacaccct
     1741 tattaaaggc atcgataagt aatgggtctc cacttagatg ggcgagaatc ctcaactcta
     1801 tctgagaata atcagcagat ataagtttat aaccctttgg ggcgatgaaa gctcttcgta
     1861 tctctttacc tatctcactt ctgacaggta tattttgaag attggggtca ctgctggcta
     1921 atcttcctgt agacgtgcct gtctgagtga atgtagtgtg taatcttcct gtatgaggat
     1981 ttataagttt aggcagggca tctacatatg tactcttcag ttttgttaat tctctgtact
     2041 ctaaaagtct ggcacagatc tcgctctcac tggctaattg acttagcact tctgcatcgg
     2101 tagaatatcc tgtcttagtt ttctttacag gaggaagctt caatttttca aacagtataa
     2161 gggagagctg tttaggagaa tttatattga attcttcacc agcaagattg tatatttctc
     2221 tttctatctg agagagggag aggtctaaca ttttagagag ttccttaaga tactcggcgt
     2281 cgattaaaac tcctttaagc tccatatctg caaggaccat ggtaagtggt aattctatct
     2341 cctgataaag cttattcata tcgtttttat cgatctcttc ttttaatatt aaacccagct
     2401 tatatatagc acaggattct ggtaaattca taccaagata tctatccgaa agcttattta
     2461 gagatacctc ctgagatggg tctattaaat atccaaggat caagacatcg agaatctcag
     2521 aagtaagttc tctctcgtaa atccctaaaa ccttccaaag agacttcata tcatatacag
     2581 caatagttct tctttcagaa aaaaggtctc ttataaaatt cttcagtacg tcttcgtcct
     2641 catctaacct gtaagtctta tcctctatag atatggatat agacttaagc ttgactggat
     2701 aaatagagag gtctaaatcg gagaacaggg acaggtaaga tgaactatca attatatcga
     2761 ggatatcttt aatacccttt ttttctgcag ctgtacctgt attatgatta taatccggag
     2821 tttctataga gaatcgcttg agtatggact tcataccgag ttcctgagct atagacttga
     2881 ctttatcaag atcaggatct ctaacagcaa tatcattcaa atcaatctct acaggaacat
     2941 ccctttttaa tgtagccaag aatagatttt ctcttacctc atcctcttta ccttcaagct
     3001 ttaccctgat tctatttgga agatactgta tatttgcaat aagttcatct acagtatcta
     3061 tatctcttaa tatctctaca gcactgctct ttcctatccc ttctatacct tttatattgt
     3121 cagaagaatc tccggctaac gccttaaaga gcggtatctt atgaggtgaa atgccaaatt
     3181 tttcttccac agcccctata tcaaagactt caacctctgt tacccctttt ctcggcacga
     3241 taactcttac atttttatc
//
LOCUS       DAOTKU010000153         3238 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_24604_length_3238_cov_3.270185, whole genome shotgun sequence.
ACCESSION   DAOTKU010000153 DAOTKU010000000
VERSION     DAOTKU010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3238
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24604_length_3238_cov_3.270185"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..162)
                     /locus_tag="P5310_04615"
     CDS             complement(<1..162)
                     /locus_tag="P5310_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009431895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HRU32676.1"
                     /translation="MGYILVLDQGTTNSRAIIFDESGNQLGDGKKELPQIFPKPGWVE
                     QNPDDIWSTI"
     gene            complement(228..303)
                     /locus_tag="P5310_04620"
     tRNA            complement(228..303)
                     /locus_tag="P5310_04620"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(268..270),aa:Val,seq:cac)
     gene            complement(404..1006)
                     /locus_tag="P5310_04625"
     CDS             complement(404..1006)
                     /locus_tag="P5310_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HRU32677.1"
                     /translation="MKKKFNQVYQFKITLRDVKPPVWRRIQVPETYTFWDLHVAIQDA
                     MGWEDAHLHEFEMRHPFEGEKVRIGIPDNDHISFVEIIPERKEKIADWFSMDNRVAEY
                     TYDFGDNWKHQIRLEKILPREEGVTYPVCISGKRACPPEDCGGAWGYARFLEAIRNPE
                     DEEHEEMLEWIGGDFDPEYFDPEEVIFDDPDERRKFAFGY"
     gene            complement(1034..1675)
                     /locus_tag="P5310_04630"
     CDS             complement(1034..1675)
                     /locus_tag="P5310_04630"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HRU32678.1"
                     /translation="MKVIDLSLPVYNEMPVYPGDPKVFFMRHRSIDPDGYNLTQILMG
                     SHTGTHLDVPLHFVEGGDSIDKFPPERFMGEAYVIDLSYKKAGEDITPEDLSPYADKI
                     QPGSRILLRTDWYKVLPDRRYFKEQPRISYELALWLVEKKISILGLETPTINPVDNKK
                     LHQTLLSGGIILVEALAHLDEIKNDKVFFIALPLKIMNGDGSPVRAVAIEDLT"
     gene            complement(1677..2456)
                     /locus_tag="P5310_04635"
     CDS             complement(1677..2456)
                     /locus_tag="P5310_04635"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HRU32679.1"
                     /translation="MESLRLIVLGMIQGLTEFIPVSSSAHLILVRNLLGWEDMGLSVD
                     MVLHLGTLLALIVYMWRDWYEILTEKRNILVLVIIATIPGAILGFLLEDMAESYFRSL
                     LVIGLAMSIFGIILFEADRKSLHNRTLNMMGLKDAIAIGLSQAMAIIPGISRSGATLT
                     GAFLCNIKREDSARFSFLLSVPMIGGAGLVSLLKMFKGVSSSVSASSMLLGLVSSFIF
                     GYLAVHFMLSYLKRYNTNIFVYYRLVLGIFLIIVSLFKPGG"
     gene            complement(2444..>3238)
                     /gene="trxB"
                     /locus_tag="P5310_04640"
     CDS             complement(2444..>3238)
                     /gene="trxB"
                     /locus_tag="P5310_04640"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HRU32680.1"
                     /translation="IENYPGFPEGISGPELAERLEAQAGKFGAKIITSQPVLNISRRE
                     EGFEIKTEMESFLGKAVIVATGTSPRKLNVPGEEEFTGRGVSYCAVCDGAFYRDRVVA
                     VVGGGDSAMDESIYLTRFASKVFVIHRRNQLRAEKILQERAFSNPKISFIWDTVVQSI
                     EGDRKVELLKLKNVKTGEISELKVDGIFVYIGSTPNSSMVKDLVELDENGFIITDNCM
                     KTSVPGLFAAGDVRNTNFRQLATAIGDGAIAANSAERYLGELSWKA"
BASE COUNT          919 a          816 c          544 g          959 t
ORIGIN      
        1 aatcgtactc cagatatcat ctggattctg ctcaacccaa cctggttttg gaaatatctg
       61 aggtagctct tttttaccat cccctaactg attacctgac tcatcaaaga tgatggctct
      121 actgtttgta gttccctgat caagaaccaa tatgtacccc ataattcatc tcctaacagc
      181 cataataaaa gctacgcctt aaattgcgta gctttaaaac tctcaaatgg tgggcgaaac
      241 aggacttgaa cctgtgaccc cctgcgtgtg aagcaggtgc tctaaccgct gagctattcg
      301 cccatttttc tttacagttt atattataac agtgaaaagc agagtttgca agacggatgg
      361 atactgagcc tgtggaaatg attaagaaat cttctcgttt tacttaatag ccaaatgcaa
      421 atttccgacg ttcatcaggg tcatcaaata ttacctcttc cggatcaaag tattctgggt
      481 caaaatctcc accaatccat tctagcatct cttcatgttc ctcatcttcg gggtttctta
      541 tagcttccag aaatcttgca tatccccacg ctcctccaca atcctcagga ggacacgccc
      601 tttttccaga tatacacacc ggatatgtaa ccccttcttc ccggggaagt atcttttcta
      661 accttatctg atgtttccaa ttatctccga agtcataagt atattctgcc actctattat
      721 ccatagagaa ccaatctgca atcttctcct tacgttctgg gataatttcc acaaaggaaa
      781 tatgatcatt atccggaatt cctatcctca ccttttctcc ctcgaaggga tgtctcatct
      841 caaattcatg gaggtgggca tcttcccagc ccatagcatc ctggatagca acgtgcaggt
      901 cccagaatgt gtaggtttct gggacctgta tccgacgcca gactggtggt ttaacatccc
      961 tcagtgtaat cttgaactga tatacctgat tgaatttttt cttcatcatt acctcccact
     1021 attgattcta gcctcaagta agatcttcta tggcaacagc ccttaccgga gacccatctc
     1081 cattcattat cttaagaggt aaggctataa agaatacctt atcatttttt atctcgtcta
     1141 aatgggcaag agcctctacc agtattatgc ctccggagag taaagtctga tgaagctttt
     1201 tattatcgac cggattgatg gttggagtct ctaatcccag tatagatatc ttcttttcta
     1261 ccagccagag ggcaagttca taactgatac ggggctgttc tttgaaatat ctcctatccg
     1321 gtagtacctt ataccagtct gttcttaaca gtattctgct ccctggctgt atcttatctg
     1381 catatggaga gaggtcttcc ggggttatat cctctcccgc cttcttataa ctcaggtcta
     1441 tcacataagc ttcacccata aatctctcgg gaggaaattt atctatggag tctccccctt
     1501 caacaaaatg caatggaaca tccagatgtg taccggtgtg tgaacccatt aagatctgag
     1561 taagattata accatcaggg tctatactcc tgtgtctcat aaagaaaacc ttgggatcac
     1621 cgggataaac aggcatctca ttataaactg gtagagaaag atctattact ttcatattaa
     1681 cctcctggtt taaataaact tactataata agaaatatcc ccagcactaa cctgtagtat
     1741 acaaagatat tggtattgta tcttttcaga taactcagca taaagtggac cgccagataa
     1801 ccaaatataa aacttgaaac taaacccagc agcatactgg aagctgaaac tgagctggat
     1861 acacctttaa acatctttag aagggatact aaacccgccc ctcctatcat agggactgaa
     1921 agtaaaaagg agaacctggc tgagtcttcc ctcttaatgt tacagaggaa ggctccagta
     1981 agggtagctc cagaacggga tatccccggg attatagcca tagcctgact gagacctata
     2041 gctattgcat ctttcaagcc catcatattc aatgtcctgt tgtgaagact tttcctatct
     2101 gcttcaaata gaatgatacc aaaaatactc atagccaaac ctatcaccag taaagaacgg
     2161 aaataactct ctgccatatc ctccaggaga aatcccaaaa tagccccggg gatagttgct
     2221 ataataacaa gtaccagtat atttctcttc tctgttaaga tctcatacca gtctctccac
     2281 atatagacta taagtgccag aagggtaccg agatgaagta ccatatcaac acttaagccc
     2341 atatcctccc atccaaggag atttcttaca aggatcagat gggcagaact gctcaccggt
     2401 ataaactcgg tcaacccctg aatcatcccc aaaactataa gtctcaggct ttccaagaga
     2461 gttctcccag atatctttcc gcactgttgg cagctatagc cccatcccct atagcagtgg
     2521 caagttgtct gaagttagta tttctcacat cgccagctgc aaataatcca ggaacactgg
     2581 tcttcataca gttatcggta ataatgaatc cattctcatc cagctccacc aggtccttca
     2641 ccatagaaga atttggagta cttcctatat atacaaatat accatcgacc ttgagctcac
     2701 tgatttctcc tgtttttaca tttttaagtt ttaacaactc cacctttcta tctccctcta
     2761 tactctgaac caccgtgtcc cagataaatg atatttttgg attagaaaaa gctctctcct
     2821 gtaatatctt ttctgccctc agctgatttc gtctatgtat tacaaaaacc ttactggcaa
     2881 acctggtaag gtaaatagat tcatccatag ctgagtctcc accgccaacc acagccacca
     2941 ccctgtctct ataaaaagcc ccgtcacaga cggcacaata agaaacccct ctacctgtaa
     3001 attcttcctc tccaggaaca ttcagctttc ttggagaagt gcctgttgct actataacag
     3061 ctttacccag gaacgattcc atttctgtct ttatctcaaa tccttcttct ctcctagaga
     3121 tattcaggac aggttgactg gttataatct ttgccccaaa tttacctgcc tgtgcctcca
     3181 gtctctctgc aagttctggt ccacttatac cctctggaaa tccagggtag ttctctat
//
LOCUS       DAOTKU010000154         3235 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_24628_length_3235_cov_3.322327, whole genome shotgun sequence.
ACCESSION   DAOTKU010000154 DAOTKU010000000
VERSION     DAOTKU010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24628_length_3235_cov_3.322327"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..771)
                     /locus_tag="P5310_04645"
     CDS             complement(<1..771)
                     /locus_tag="P5310_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRU32681.1"
                     /translation="MMLVTFLGAAGEVTGSCYLLETDRYKYLVDCGMFQGKDEERNNA
                     SFSFLPSSIESVILTHAHLDHSGRIPKLVKDGFRGKIYATIPTIELCEILWLDTVKLM
                     REETERINRKNKRAGKSMTEPLYTDEDVQLALSLFEPVPYDSIINLKDIRVRFRDASH
                     VLGSSSIEVWDEETKIVFSGDIGQWEGVMNDSPALIEQADYVVIESTYGDRLHRSLED
                     TRKEFKDTIQSAILERGKVLIPSFVVDRAQRVIYELKLL"
     gene            922..1653
                     /locus_tag="P5310_04650"
     CDS             922..1653
                     /locus_tag="P5310_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013429603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HRU32682.1"
                     /translation="MHIKEILTEKNTDYSSNSIPTIVFLGDSVTHGCFEVIEGNNKPL
                     EVICDFEAVYHNQLKKLLNMVFPFTPINTINAGISGDTAAGGMQRLDRDVLSFNPHLV
                     VICYGLNDSNRGREKLHEYRDSLLGIFSKLKANNTEIVFMTPNMKNTYISPLIKNPLL
                     REMARINMETQLDGTFNEYINCVREICRKEEVTICDCYSKWQKMYRDNIDTTILLSNL
                     INHPTREMHKLFAWSLFELIVFSDK"
     gene            1673..2662
                     /locus_tag="P5310_04655"
     CDS             1673..2662
                     /locus_tag="P5310_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013291127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRU32683.1"
                     /translation="MKICIIGSSGHYGYALNGIRADGQSDIAGLSKGDKDENIEGLIK
                     QVREAGYQPEVYDDPVEMIDELKPDIAVINTFFHKNSKLAIEAMKRGINVYIEKPVAL
                     TLEELDRLIEVYNQTQVKLSTMLGIRYSPHFWTAHNLVKNNKIGEIRLIHAQKSYKLG
                     VRPEFYKRRETYGGTIPWVGIHAIDWIYWFSGKKFKNVYASHSSLFNNNNGELEVTAV
                     CNFTLEDEVLATVNIDYLRHSSAPTHGDDRVRIVGTEGVVEVINEKVFLLGQDTDGQK
                     EVPPEQGPVTFLDFIWEVRGIGKCLVSAEDSFYVTRASLLARESADRNEIISF"
     gene            2674..>3235
                     /locus_tag="P5310_04660"
     CDS             2674..>3235
                     /locus_tag="P5310_04660"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HRU32684.1"
                     /translation="MDLGLPGLSEEIESILEKIVEVFQDTLKDDLIGIYLHGSLAMGC
                     FNPEKSDIDFLVVVKKKLSAEIKRSVVKSTLNLANSDGIPEKGLEFSIIQEKYLRDFI
                     HPMPFEFHYSKDWRKAYEEDKVDLNKENTDRDLAAHITVIINRGICIYGKSIKEVFPY
                     RIPVKYYIDSLLYDAESIKEDIHRNPV"
BASE COUNT         1098 a          585 c          640 g          912 t
ORIGIN      
        1 caagagcttc aactcgtata taaccctctg agctctatcc accacaaagg agggaataag
       61 gacctttccc ctttctaaga tcgcactttg gatagtatct ttaaactcct tcctcgtatc
      121 ttccagtgac ctgtgaagtc tatcgccata tgttgactct ataactacat aatcagcctg
      181 ttctatcagg gcaggagagt cattcataac cccttcccac tgccctatat ctccagaaaa
      241 aactatttta gtctcctcat cccatacctc tatagaagaa gagcctagaa catgagaagc
      301 gtctctgaat ctgactctaa tatctttcaa gtttatgata ctgtcatacg ggactggctc
      361 aaatagactg agagcaagtt gaacatcctc atcagtatac agaggctctg tcatagactt
      421 ccctgccctc ttattctttc tgtttatcct ctcggtctct tccctcatta actttactgt
      481 atcaagccat aagatttcac ataattctat tgtgggtata gtagcgtata ttttacctct
      541 aaaaccatcc ttaacaagtt taggaattct accagaatga tcaaggtgag catgagtcaa
      601 aattacagac tctatagaag atgggagaaa actaaaggag gcattgtttc tctcttcatc
      661 tttgccctga aacataccac aatctactaa atacttgtat ctatctgtct ctagcagata
      721 acaggagcct gtgacttctc ctgctgctcc caaaaaagtg accagcatca ttcatcctcc
      781 aataaaaatt taatattctt ctatattata acatcgataa atgtatattc tcagtttgac
      841 atctctcttt tttgtagtat agtaattaat aacaaaatat aaataaaaga cactgattcc
      901 gtaaagggag atgattttac aatgcacata aaagaaattc tgacagagaa aaatacagat
      961 tacagctcaa attcaatccc taccattgtt ttcttagggg atagtgtgac acatggatgt
     1021 tttgaagtga tagaagggaa taataaacct ctggaagtta tctgcgattt cgaagcggtc
     1081 tatcataatc agttaaaaaa gcttctcaat atggtattcc cgtttacccc aataaatact
     1141 ataaatgctg gtataagcgg tgatactgca gcagggggaa tgcagagact tgatagagac
     1201 gtattgtcat ttaatcctca cctggtcgtc atctgctatg gattaaatga cagcaataga
     1261 ggcagagaaa aactccacga gtatagagac tcactactcg ggatattctc aaaattaaag
     1321 gcaaataata ctgagattgt atttatgact cctaatatga agaacacgta tatctcgccc
     1381 cttataaaaa atcccttatt gagagaaatg gcaagaataa atatggaaac tcagctagac
     1441 ggaacgttca atgagtacat aaactgcgta agagagatat gcaggaaaga agaggttacc
     1501 atttgtgatt gttacagtaa atggcaaaaa atgtatcggg ataatattga tacaactata
     1561 ctcctctcta acctgattaa tcatccaacc agagagatgc ataagctttt tgcgtggtca
     1621 ctgtttgagc tcatagtgtt ttctgacaaa taatggaaag gatgtgatat gagtgaagat
     1681 atgtattata ggttcaagcg gacattatgg atatgcttta aacgggataa gagctgatgg
     1741 acaatcagat attgctggtt taagtaaagg ggataaagac gaaaatatcg agggattgat
     1801 taagcaggtc cgggaggctg gatatcagcc tgaagtttac gatgaccctg tggagatgat
     1861 agatgagctg aaacctgata tagctgtaat aaatacattt ttccataaga actctaaact
     1921 cgcaatcgaa gcgatgaaaa gggggattaa tgtatatata gaaaagccag ttgctctgac
     1981 actggaagag ctggacaggt taatagaagt ttataatcaa actcaggtta aactatcaac
     2041 tatgctcgga ataagatatt cccctcattt ctggacagcc cataatcttg tgaaaaacaa
     2101 taagataggt gagatcagac taatacatgc acagaaatcc tataaattag gggtaagacc
     2161 tgagttttat aaaaggagag agacctatgg cggtacaatt ccctgggtag gaatacacgc
     2221 aattgactgg atctactggt ttagcgggaa aaaatttaaa aatgtatatg cctcacattc
     2281 aagcctgttt aacaataata acggagaact tgaagtaaca gctgtctgca attttaccct
     2341 cgaggacgag gtcctggcaa cagtcaacat agactatctg agacattcaa gtgcacctac
     2401 ccatggtgat gatagggtca gaattgtagg gacagaagga gtggtggagg taataaacga
     2461 aaaggtattt cttttaggtc aggatacaga tggacaaaaa gaagttccac ctgagcaggg
     2521 tccggttaca tttttagatt ttatctggga agtgagagga atcgggaaat gtctggtaag
     2581 tgccgaagat agtttctacg ttacaagagc cagtcttcta gcccgtgaat ctgcagatag
     2641 aaatgagata atttcattct gaaggaatta actatggatt taggacttcc aggtctttct
     2701 gaagagatag aatcaatact ggaaaagatt gtggaggttt ttcaggatac actcaaagat
     2761 gatttaatcg gtatatatct tcatggctca ttagctatgg gatgtttcaa cccagaaaaa
     2821 agtgatatag acttcctggt ggttgttaaa aagaaattat cagcagagat aaaaagatct
     2881 gttgtaaaat caactctaaa cctggcaaac tcagatggaa ttccagaaaa ggggttagag
     2941 ttcagtatca tacaggaaaa atatctgagg gattttattc atcctatgcc tttcgaattt
     3001 cactactcaa aggactggcg caaagcctac gaagaagata aagtagacct taataaagaa
     3061 aacacagaca gagaccttgc tgcccatatt acagttatca taaacagagg gatatgtatc
     3121 tatggaaaat ctataaagga agtcttccca taccggatac cggtcaaata ttatatagat
     3181 tctcttctct atgatgcaga atcaattaaa gaagatatac atagaaatcc ggtat
//
LOCUS       DAOTKU010000155         3230 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_24674_length_3230_cov_2.881890, whole genome shotgun sequence.
ACCESSION   DAOTKU010000155 DAOTKU010000000
VERSION     DAOTKU010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24674_length_3230_cov_2.881890"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1713
                     /locus_tag="P5310_04665"
     CDS             <1..1713
                     /locus_tag="P5310_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004083312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRU32685.1"
                     /translation="VHGDIALVEAALSVRCFFRLVDIGVPFPHNSYGEYVGYKTDHDP
                     RQRATSAGPLTSKYMTERLQEEVERKGIKVFDGYQIIGILTDRSRTRTVGLLALNLNN
                     LENPDKRYVLFNCTNIIYATGGPAGMYRSYVYPESQTGSTGIALEVGVRGRNLTESQF
                     GIASIKFRWNLSGTYQQVLPRYVSTNQDGGDEKEFLTEYFSDTGKMLTAIFLKGYQWP
                     FDPRKINNFGSSLIDILVHYERVVKGRRVFLDFTKNPLDKDFRFDLLEKEAYEYLEKS
                     NALFGTPIERLSHMNQPAIELYKNHGIDLSKEYLEIAVCNQHNNGGLAGNIWWESNIK
                     HFFPVGEVNGSHGIYRPGGSALNSGQVGSTRAVEYISACYREDPMHIEEFLSTVKDQI
                     GKKVELGESFLSNLSEGSNIYEIRRDIGERMDMVGGIIRSLESARKAAKEVKDTLNRI
                     KDTTRISSVRELPYAYQNYDLLLTQYVYLSAIANYIENRGRSRGSYLVYDREGELPLP
                     VLPEAFRFSLDDGRLSEIIQEVAYRDGECYFEWIPVRPIPKGDDWFENVWNNFRRGEI
                     YRKE"
     gene            1720..2943
                     /locus_tag="P5310_04670"
     CDS             1720..2943
                     /locus_tag="P5310_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012003179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRU32686.1"
                     /translation="MEKISVLLIGIGGYGNNYVRAMLEEGEAHNAEIAGVVDPYPESC
                     RYLNELRNRRIPIFSRLEDFYANNRADLAVISSPIQFHCRQTCMSLNYGSNVLCEKPA
                     AATVQEVDQMIEARDKSGLTVAVGFQWAYDPPFQSLKRDVMSGLLGRPERLRTIVLWP
                     RDTAYYKRVWAGKKRDSDGSWILDSVANNATAHFLHNMLHLLGEKEDASTRPVEVTAE
                     LYRANPIENFDTSAIRVLTDANAEILFYASHAVKNLFGPAFLYEFEDAMVAYNEPGLP
                     ESSNKLVARFKDGRLKEYGSPEHGSVRKLWVIMDAIRGLTSIPCPLESAKAHTLCING
                     AQDSMPDIVDFPSEFIRREGDPVINWVEGLEDVLKGCYNKGTLPSEDNIVWAKSGRTI
                     SLIDYNYFPKDAVDK"
     gene            2986..>3230
                     /locus_tag="P5310_04675"
     CDS             2986..>3230
                     /locus_tag="P5310_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32687.1"
                     /translation="MYREVIFFTLLALTVILALPRSAFAWGGHREVTYYTVKDIAWIN
                     SYGKIKITPFIYEDQGKRKTPYSIPYKEGYIGDTTDAL"
BASE COUNT         1040 a          540 c          786 g          864 t
ORIGIN      
        1 gtacacggag atatcgcact tgtcgaagct gctctttcag ttagatgttt ttttagactg
       61 gtagatatag gtgtcccttt cccccataat agttatgggg aatatgttgg ttacaagaca
      121 gaccatgacc cacgccagag agctacatct gcaggacccc ttacttcgaa gtatatgact
      181 gagagattac aggaagaggt cgaaagaaaa ggtattaagg tctttgatgg atatcaaata
      241 atagggatac ttacagacag gtccagaaca aggactgttg gattattggc tcttaatctg
      301 aataacctgg agaaccctga taaacgttat gtgttattta actgtaccaa tataatatac
      361 gctaccggtg gaccggcagg tatgtacagg tcatatgtat atccggaaag tcagacaggg
      421 tctactggta tcgccctgga agtaggggtc agggggagaa atttaactga atctcaattt
      481 ggaatagcct ctataaaatt cagatggaat ctctcaggaa catatcagca ggtgctcccc
      541 agatatgtat cgaccaacca ggatggtggc gatgaaaaag aatttcttac agaatacttc
      601 tccgacacgg gtaagatgct taccgccata ttcctcaaag gttatcaatg gccatttgat
      661 ccaagaaaaa taaataactt tggatcatct cttatagata tcttggttca ctatgaaagg
      721 gtagtgaaag ggaggagggt atttctggat tttacaaaaa atccccttga taaagacttc
      781 aggtttgacc tcctggagaa agaagcatat gaatatctgg agaagtcaaa tgcgttattc
      841 gggacaccca tagagagact atcgcatatg aatcagccgg cgatagaatt atataaaaat
      901 cacgggatag atctatctaa agagtatctt gagatagcgg tatgtaacca gcacaataat
      961 ggtggattag ctgggaatat atggtgggag agtaatatta agcatttctt ccctgtaggg
     1021 gaagtaaatg gctctcatgg gatttatcgt ccaggaggaa gtgcattaaa ttcaggtcag
     1081 gtcggcagta ccagagcggt agaatatata tctgcctgct acagagaaga ccccatgcat
     1141 atagaagaat tcttaagtac agtaaaggac cagataggaa aaaaggtaga attgggagag
     1201 agttttctat ccaatttaag tgagggtagc aatatctatg aaattagaag agatatcggt
     1261 gaacgtatgg atatggtagg aggtataata cgatctctgg agtctgctag gaaggctgca
     1321 aaagaggtta aagataccct gaatagaata aaggatacta ccagaatctc ttctgtcaga
     1381 gaacttccat atgcctatca aaactacgac ctgcttttaa cccagtatgt ctacctctct
     1441 gcaatagcaa attatataga gaatagaggg agaagcagag gttcgtatct ggtatatgat
     1501 agggagggag agttacctct acctgtcctt ccagaagcct tcaggttctc tctggatgat
     1561 ggaagactct ctgaaattat acaggaggta gcttataggg atggagaatg ttattttgaa
     1621 tggattcctg ttcgtccgat accaaaaggt gatgactggt tcgagaatgt ctggaacaat
     1681 tttagaagag gagaaatcta cagaaaggaa taattaacta tggaaaagat atcagttttg
     1741 ttgataggga taggcggtta cggcaataat tatgtaagag ccatgttaga ggaaggagag
     1801 gctcacaatg cagagatagc aggcgtagta gacccatatc cagagagttg tagatatcta
     1861 aatgaattga gaaatagaag gattcccata ttttctcgtc tagaagattt ttatgccaat
     1921 aacagggctg atctggcggt catatcatca cccatacagt tccactgcag acagacatgt
     1981 atgagtctaa actatggaag taatgtatta tgtgagaaac ctgcagctgc aactgttcaa
     2041 gaggttgatc agatgataga agccagagat aaaagtggac ttactgtggc tgttggtttt
     2101 cagtgggctt atgaccctcc atttcaatca ttaaaaagag acgtaatgag tggtttactg
     2161 ggcagaccag agaggctcag aacaattgtt ctctggccca gggatacggc gtactataaa
     2221 cgggtatggg ctggaaagaa gagggacagt gatggaagct ggatactgga tagtgtggct
     2281 aataatgcta ctgctcattt cctccataat atgctgcatc ttctggggga aaaggaggat
     2341 gcaagtacca gacctgtaga agtgacagct gagctgtaca gagccaatcc tatagagaac
     2401 tttgacacct cagcgatcag ggttttaact gatgctaacg ctgagatact tttctatgcc
     2461 agtcatgcgg taaagaacct atttggacct gcatttttgt atgagtttga agatgctatg
     2521 gttgcctata acgaaccagg gcttcccgaa agttcaaata aacttgtagc caggtttaaa
     2581 gatggaagat tgaaggagta tggaagtcca gagcatggaa gcgtaagaaa actctgggta
     2641 ataatggatg caattagagg gttaacgagt atcccctgcc ctcttgaatc cgccaaggct
     2701 cataccctct gtataaatgg tgctcaggat tcaatgccag atatagtgga ttttcccagt
     2761 gaatttataa ggagagaggg agatccggtt ataaactggg tggaaggttt agaagatgta
     2821 ctgaaaggct gttacaataa gggaacactt ccttctgaag ataatattgt atgggcaaaa
     2881 tctggtagaa cgatctcgtt gattgactat aattatttcc ctaaagacgc tgttgacaaa
     2941 tagtatcaca ctaatataaa cttatagaaa aatagagagg ctcatatgta cagagaggta
     3001 atatttttta cacttttagc tttaaccgtg atactggctc tgccaagatc tgcctttgcg
     3061 tggggagggc atagagaggt tacatattat actgtaaagg atattgcatg gataaacagt
     3121 tatggtaaga taaaaattac cccgtttatc tatgaggacc aggggaagag aaagacaccc
     3181 tatagtatac cttataagga aggctatata ggagatacta cagatgcact
//
LOCUS       DAOTKU010000156         3223 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_24731_length_3223_cov_3.008838, whole genome shotgun sequence.
ACCESSION   DAOTKU010000156 DAOTKU010000000
VERSION     DAOTKU010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24731_length_3223_cov_3.008838"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1346)
                     /locus_tag="P5310_04680"
     CDS             complement(<1..1346)
                     /locus_tag="P5310_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32688.1"
                     /translation="MTEKRGIYKKVSPMYKLRVFFVLMILSLSLFSQYASSQVGVDIN
                     FELGFDGMVKIGRWNPVRVTVRTYSVPFKGSIEIEITEGSIILANITSTRLKSDVDLP
                     VYSEREFLFPVPLSDVRHPVKVSVLSPDGRVIKSTSYDIKNLDMRLPILCLVGSRSLP
                     YRGSGARVLALGADVLSGKPFWILDPFDALVLDYSSFKSLKDILDKWSLWGGKIIGEG
                     DTIEAVSYRGDTYPGDVDLSSLVSDIIDEELIPLPSKLYASLTLFFYILFFVFLFYYS
                     KRSFLQKILIYLLVSLAFSCFLFITTLNVKEKSDISIQINIMESDKNYPYAKVYSGLA
                     IFSPYDKVIRFGYNPGDFVFWTGRKSKDIAETTFILKEEDIFAELNLGPDKIGLLRGW
                     GAVNFDLKGYISGENLNLVNSSPYTVEDAYLLYRGGYIHLGDIGVGDKTIKISNER"
     gene            1441..2481
                     /locus_tag="P5310_04685"
     CDS             1441..2481
                     /locus_tag="P5310_04685"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRU32689.1"
                     /translation="MESRIENWIKTHLSCVRTTSAELTYERMDSQAYHNLPVIYQPLD
                     VNNKSHWYDIAICSAFSYAVRGSRIILDVGPGDGWPSLRVAHNFEKVVGIDPSHRRVS
                     IQRENARRLGIRNVEFLEMDAINMIFPDNSFDGVIAANSIEQSNNPSLALEEVFRVLK
                     PGGKLAMIFENYDHYIPSKDGDALLWAEIIANECILFYSLKEKNPPREVKYALFLDAE
                     WLMQEHDLRRKINELSEKKYSLYNDERFGTSFFERLAPFVIQTKYYELSHFTSNLLQE
                     TLSKTGFINIRGVDVNIPQLSSFFDIAKNEGKLESLEPMMETIGEIFGIMAVESSVPM
                     VLDFVIAQKPPL"
     gene            complement(2476..>3223)
                     /locus_tag="P5310_04690"
     CDS             complement(2476..>3223)
                     /locus_tag="P5310_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32690.1"
                     /translation="DEVLDQNGFGFIEHPFFYGNHHIHPFNILPNPWTDWNVRGFTGM
                     SVFNYTSDGGERMRLYNFLIYYTFPGLDKDRPNARTIEKWDALNLSRKTVGIGTADAH
                     LYRTKAGPLHISVWPFSYYFNSIRTHILSSSPLSPDTVYSALKSGHCYISHNYLGNSR
                     GFLFWAENKDSRVIMGDEIELKGDTELHIYSPLTAKIKLLKNGVTLIERVTDQLDYPV
                     ESPGVYRVETSIYHGFFWKPWVYTNPIYVL"
BASE COUNT         1006 a          651 c          659 g          907 t
ORIGIN      
        1 aatctttcat tgctgatttt aatagtttta tccccaaccc caatatctcc aagatggata
       61 tatccacccc tgtataagag ataggcatcc tccaccgtat atggagaact gttgactaga
      121 ttaaggttct caccagagat atagcccttt aaatcaaagt taaccgctcc ccaccctctg
      181 agaagcccaa tcttatcagg accaagattt aactcagcaa agatatcctc ttccttcagg
      241 atgaaagttg tctctgcaat atctttgctc tttctccctg tccagaagac gaagtcccca
      301 gggttatatc caaatcttat aaccttatca taaggagaaa agatggcaag acctgaataa
      361 actttagcgt atggatagtt tttatcagac tccattatat taatctgtat ggatatatca
      421 ctcttttctt taacatttag agttgtaata aataagaagc aggaaaaggc aagactgacc
      481 agaaggtata tcaggatttt ctgtaagaat gatctttttg aatagtagaa gagaaaaacg
      541 aagaaaagta tataaaagaa cagggtgaga gatgcatata atttagaggg taacggtatc
      601 agttcttcgt caatgatatc agacaccaga gaggacaggt caacatctcc aggataagta
      661 tcaccacggt aagagaccgc ttcgatggta tctccctcac ctattatctt tccaccccat
      721 aatgaccatt tatcaagaat atctttcaag cttttaaaag aagagtaatc taataccagt
      781 gcatcaaacg ggtccagtat ccagaatggt ttaccagaaa gtacatcagc ccctaaagcc
      841 aggaccctgg caccgctacc tctgtacggt aagctacggc taccaaccag gcataatatg
      901 ggtaacctca tatccaggtt ttttatatca taactggtag actttataac tcttccatct
      961 ggagaaagga cggaaacctt taccggatgt cttacatccg agaggggaac aggaaagaga
     1021 aattccctct cagaatatac aggcaggtcc acatctgact tcaacctggt agatgtaata
     1081 tttgccaaaa ttatgcttcc ctctgttatt tctatttcta tactaccttt gaaggggaca
     1141 ctgtaggtac gtacagttac tcttacagga ttccatctac ctatcttaac cataccgtca
     1201 aaaccaagtt caaaatttat atctaccccc acctgagagg aggcatattg agagaataga
     1261 gataaagata aaatcatcag gacaaaaaag accctgagtt tgtacattgg actcaccttc
     1321 ttatatatac ctcttttttc agtcatctat aattataata cctgttaata gatgacatca
     1381 atcgtttctg tgggggcatc acctgtcgca gaacgttaac aatatgctat aatgtagact
     1441 atggagagta gaattgaaaa ctggattaag acgcatctat cctgtgtaag gaccaccagt
     1501 gcagagctga catatgagag gatggattcc caggcttatc ataatctccc agttatatac
     1561 cagccgcttg atgttaataa taaatcacac tggtatgata tagctatctg cagtgcattc
     1621 agctatgctg taagaggctc ccggataata ctggatgtag gaccgggaga cggctggcct
     1681 tctctcaggg tggctcataa ctttgaaaag gtcgtgggga tagacccatc tcacaggagg
     1741 gtcagtatcc agcgagaaaa tgccaggaga ctgggtataa gaaatgtgga attcctcgag
     1801 atggatgcga taaatatgat cttcccggac aatagcttcg atggggttat cgctgctaac
     1861 tctatagagc agagtaacaa tccatcactg gcattggaag aggtattcag ggtattgaaa
     1921 cctggtggga aattagccat gatttttgaa aattatgacc actacattcc atcaaaggat
     1981 ggggatgcgc ttctatgggc tgagattata gccaatgagt gcatactatt ctactcgtta
     2041 aaggagaaga atccacccag agaggtaaaa tatgcactgt tcctggatgc agaatggctt
     2101 atgcaggagc atgatttaag gagaaagatt aacgagttgt ctgagaaaaa atatagcctt
     2161 tataatgatg aaagatttgg tacctcattc tttgagaggt tagctccgtt tgttattcag
     2221 acaaaatatt acgaactctc ccattttaca tcaaatttgc tccaggaaac cctctccaag
     2281 actggtttta taaatataag aggggtggat gtaaatatac ctcaactctc gagtttcttc
     2341 gatatagcta aaaatgaagg gaaattagag agtctggaac ctatgatgga gacgatagga
     2401 gagatatttg gaataatggc tgtagaatcc agtgttccta tggtactgga ctttgtaata
     2461 gcccaaaaac cgcctctata agacatagat cgggttggtg tatacccagg gtttccagaa
     2521 aaatccgtga tatatactgg tttctaccct gtatactccg ggtgattcga ccggataatc
     2581 cagctgatct gttactcttt caatgagagt cacaccattc tttagcaatt taatcttagc
     2641 cgttaggggt gaatagatat ggagttcagt atctccctta agttctatct catctcccat
     2701 aattaccctg gaatctttat tttcagccca gaacaaaaaa cccctggagt ttccaagata
     2761 gttatgggat atataacagt gcccactttt aagggcagaa tatactgtat ccggacttaa
     2821 tggagaactg gacaatatgt gtgtccttat agagttaaaa tagtaagaga atggccagac
     2881 agaaatatgg aggggacctg cctttgtcct gtatagatgt gcatctgccg tacctatacc
     2941 aaccgtcttt ctggagaggt taagtgcatc ccacttctct atggtcctag cattgggacg
     3001 gtccttatct aacccaggga atgtatagta tataagaaag ttatacagtc tcatccgctc
     3061 cccgccatcg gaggtatagt taaagacaga cataccggta aaacccctga cattccaatc
     3121 tgtccatgga tttggaagta tattgaatgg gtggatatgg tggtttccgt agaagaatgg
     3181 atgttctata aaaccgaagc cattctggtc caatacctca tct
//
LOCUS       DAOTKU010000157         3207 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_24883_length_3207_cov_3.318528, whole genome shotgun sequence.
ACCESSION   DAOTKU010000157 DAOTKU010000000
VERSION     DAOTKU010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3207
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24883_length_3207_cov_3.318528"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(108..1547)
                     /locus_tag="P5310_04695"
     CDS             complement(108..1547)
                     /locus_tag="P5310_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012582455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HRU32691.1"
                     /translation="MERSYIFLFNLATKYLFPDLKVIIEHSYHKSLYGRFKDYIPTIE
                     DLSKIEEKMREIAEEDIPIKKEVYSRESAIKLFEERQEEDKIGLLKYYTGKEVILYHI
                     DDYYEYSYLPVVPSTGYLKTFALKLYQPGFLIILPEDKDINKVAEFKEVVKLFQAFYE
                     YKNWLSILGLNNVSSLNSAIENGEISEIIKVAEALHEKKIAQIADEICSRLPDLKLVL
                     IAGPSSAGKTTFTKRLQIQLKVNGIKPIVIEMDDYFLPPQEVARDEYGNYDFDSPNAL
                     NINMLAEHLNILMNGGEVELPKYNFQKKRSERSGRYIKIDKNSLLVMEGIHALNPQIT
                     PEIPGYMKYHIFVSALTHLNLDNQNRVPTTDARLIRRMVRDSIFRGHGVLDSIRQWPS
                     VRRAEDLYIFPNQEKADAMFNSALVYEFSVLKNFIEPILKAIPPYYREYPEVKRILDI
                     LSHFIPLSPTEVPMTSILREFIGGSSFVY"
     gene            1859..2257
                     /locus_tag="P5310_04700"
     CDS             1859..2257
                     /locus_tag="P5310_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HRU32692.1"
                     /translation="MEAKVTWQKEGLRFVGHIDGSPDVIMDSENGPTPMELVLMALGG
                     CTGMDVISILQKMRQDVKDFEVNLSAERAQEHPKIFTRINLEYVVYGSNIEENSLKRA
                     IELSQEKYCGVGNMLKKAAEITYSWKIVNS"
     gene            complement(2283..>3207)
                     /locus_tag="P5310_04705"
     CDS             complement(2283..>3207)
                     /locus_tag="P5310_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HRU32693.1"
                     /translation="IGIIVIAATNRSDILDPSLLRPGRIDRKVVVDNPDLEGRLEILK
                     IHARGKPLAEDVNLQVIAQRTPGFSGADLANLLNESALLAARRGKNKITMEELEEAID
                     KVIAGPERKSRVLSEKEKELIAYHEVGHALVTKFLPNADKVHRISIIPRGMALGYTLH
                     LPTEDKHILTKAELLDKIVTALGGRAAEEIIFNETTTGAQNDLEVATEIARRMVTEFG
                     MSEELGPLTFGKKQGQVFLGRDLIESRNYSEQVAFMIDREIRRIIESCYDKAKDILVK
                     NKETLIKIASILKEKETIEAEEFDKLIAQPA"
BASE COUNT          850 a          672 c          572 g         1113 t
ORIGIN      
        1 gttctggagt cagtggtctt actgctaaaa atgttatgta tatgtcatct tcaaaaacta
       61 tatatccttc tgcatcacct tttataatct ttcagaatat acattctcta gtagacaaaa
      121 gagctccctc ctataaattc tctaaggatt gatgtcattg gcacttctgt tggtgagaga
      181 gggataaagt gagagagtat atccagtatc ctctttactt ccgggtactc tctgtaatat
      241 ggggggatag ctttcagtat gggttctata aagttcttca gtacggaaaa ctcgtaaact
      301 aatgctgagt tgaacattgc atctgctttt tcctggtttg gaaagatata caggtcctct
      361 gctcttctca ctgaaggcca ttgacgtatg gaatccaata ctccatgccc tctgaatata
      421 ctgtctctta ccatacgtct tatgagtctg gcatcggtag tggggactct attttgattg
      481 tctagattga ggtgcgttag agcactgaca aatatatgat acttcatata tcctggaatt
      541 tctggcgtta tctgtggatt cagtgcatga atcccctcca taacgagaag actattctta
      601 tcaatcttta tataccttcc agacctttca cttctcttct tctgaaagtt atacttcgga
      661 agttccactt ctccaccgtt cataaggata ttgagatgtt ctgcgagcat atttatattt
      721 aatgcgtttg gactgtcgaa atcatagttt ccatattcat ccctggcaac ctcctgtgga
      781 ggaagaaaat agtcgtccat ctctataact ataggtttta ttccatttac ctttaactgt
      841 atctgaagtc ttttggtaaa agttgtcttt cctgctgatg aaggtcctgc tataaggaca
      901 agctttaaat cagggagtct tgagcatatc tcatcagcta tctgagctat cttcttttca
      961 tgtagagctt ctgccacctt tattatctca gagatctccc cattttcaat tgcagaattc
     1021 agagatgata cattattaag tcccagtata ctgagccagt tcttatattc gtagaaagcc
     1081 tggaacagtt taactacctc tttaaactct gcaaccttat ttatatcttt atcctcaggc
     1141 agtattataa gaaatccagg ctgatataac ttcagggcaa atgtcttcag ataaccagtc
     1201 gagggtacca cagggagata agaatattca tagtagtcat ctatatggta tagtattacc
     1261 tctttaccgg tataatattt cagtaatcct atcttatcct cttcttgcct ctcttcaaag
     1321 agttttatgg cagactctcg actgtaaacc tcctttttga taggtatatc ttcttcggca
     1381 atttctctca ttttctcttc tatttttgac aggtcttcta tggttggtat atagtctttg
     1441 aacctcccat aaagactctt gtggtacgag tgctctatta tgacctttag atctggaaac
     1501 agatattttg ttgccagatt aaaaagaaat atataacttc tctccaatac tctccttccg
     1561 taattatttt ttacccctat caggtcaatt gaaatatcct catctaaaat gtaatttaaa
     1621 tcttcaattc tatcgcctat ctgaaccgct acagggatat catcttctcc taaaagagta
     1681 acctctacct gtggaaattt ctccaggaac tctcttactg ataatgggct atctacttct
     1741 agatttatag cgcctcttaa tcttatctct atttctattc atctccttta aattccagtt
     1801 ttatagatag attataccat tttattgtat aattataaaa aatcatcagg aggttcttat
     1861 ggaggctaag gtcacttggc agaaagaagg gttaagattt gtcggacata tagatgggag
     1921 tcctgatgtt atcatggata gtgaaaatgg tccgacacct atggaactgg ttcttatggc
     1981 tctgggtgga tgcaccggga tggatgtgat atcaatattg cagaaaatga gacaggatgt
     2041 aaaagatttt gaggtaaatc tatcagcgga gagagcccag gagcatccaa agatatttac
     2101 caggataaac cttgaatatg tagtttatgg ctctaatata gaagagaact ctctaaaaag
     2161 agccatagaa ttatcacagg agaagtactg tggtgtgggg aatatgctaa agaaggcggc
     2221 tgaaattacc tatagctgga agatagtgaa cagctaaaaa gagcaggggc tccaagcccc
     2281 tgctaagctg gctgagctat aagtttatca aattcctctg cttctatcgt ctctttttct
     2341 ttcaggatac tggctatctt tatcaatgtc tctttgtttt taacgagtat atccttagcc
     2401 ttgtcgtagc aactttcaat aattcttctg atctctctat ctatcataaa tgctacctgc
     2461 tcactgtagt ttctactttc tataaggtct ctacctaaaa agacctgccc ttgtttcttc
     2521 ccaaaggtta atggaccaag ctcttcactc ataccaaact cggtaaccat tctcctggca
     2581 atctctgtcg ctacttccag atcgttctga gcaccggtag tggtttcgtt aaatattatc
     2641 tcctcggcag ctctacctcc cagagctgtt actattttat ccagtaactc tgctttggtg
     2701 aggatatgtt tgtcttctgt gggtaaatgg agcgtatatc ccagagccat tcctctgggt
     2761 attatggaaa ttctatggac tttatccgca tttggcaaga attttgtaac cagtgcatgc
     2821 ccaacctcgt ggtaggctat caactccttt tccttctctg agagcactct actttttctc
     2881 tccggcccgg ctattacttt atctatagcc tcttccagtt cttccatagt tattttattt
     2941 ttacctctcc ttgctgcaag cagtgctgat tcatttagaa gatttgccag atctgctcca
     3001 gagaatccag gtgtcctctg tgctattacc tgtagattta catcttctgc caggggtttt
     3061 cccctagcgt gaatctttaa tatttctaat ctaccttcta agtctggatt atctaccact
     3121 acttttctat ctatccttcc tggtctcagt agagaaggat caagtatatc agatctatta
     3181 gttgcagcta tgactatgat tcctata
//
LOCUS       DAOTKU010000158         3196 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_24997_length_3196_cov_2.943012, whole genome shotgun sequence.
ACCESSION   DAOTKU010000158 DAOTKU010000000
VERSION     DAOTKU010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24997_length_3196_cov_2.943012"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /locus_tag="P5310_04710"
     CDS             <1..704
                     /locus_tag="P5310_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HRU32694.1"
                     /translation="ELFRQEGVRNIQGYNLKKKDSPLYYIVVIVDELADLMMVAPKEI
                     ERLICRLAQMARATGIHLIIATQRPSVDVITGLIKANFPSRIAFAVSSQADSRVILDI
                     GGAEKLLGKGDMLYSPIWAMRPVRVQGAYVSDEEIEKVLNFWKLQGTPNYAVTQEELE
                     SEDIISSSDSESNDEYLEEAKKILKQKGGISTSLLQRMLRIGYGRAARIIDLLEAEGY
                     VERGEDGRVRVVRLD"
     gene            738..1724
                     /locus_tag="P5310_04715"
     CDS             738..1724
                     /locus_tag="P5310_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018963163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HRU32695.1"
                     /translation="MKKFLVGILVLTLVFGFSSFSYAAKAKVGIVYDVGGRGDKSFND
                     AAYKGLQDAVKKLDIEGKDVEPGEGGANREELLRLLAEQGYDLVIGVGFLFTDALVKV
                     AKDFPNTKFAIVDGVIEDLPNVRSLVFKEHEGSFLVGAIAGLMTKTNKVGFVGGMDMS
                     LIHKFEVGYRAGVKYVNPKAEIFVNYIGVTGDAFKDPVKGKELALSQFKQGADIVYHA
                     SGASGEGVIEAAKEMKKFAIGVDSNQNWIAPGYVLTSMLKKVDVAVYKTIEDLVNGKF
                     TGGVKVYGLRENGVGVAYDKYNEKLIPESVRRRVAQIRLDIIAGKIKVPETK"
     gene            1727..>3196
                     /locus_tag="P5310_04720"
     CDS             1727..>3196
                     /locus_tag="P5310_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018753040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRU32696.1"
                     /translation="MSEEFILETEGITKHFPGVLANDDVSIQIREGEIHSIIGENGAG
                     KTTLMNIIYGIFRPDRGKIFYKGREVEINNPSTAISLGIGMVHQHFMLVPPFTVWENI
                     ILGREVLKRGVLDKDSVSEDLNKLMSDYRLSVPLEEKIEDLPVGICQKVELIKLLYRG
                     AEVFILDEPTALLVPQEIEELFEILNNLKRQGKTIIFISHKLKEVLRISDRITVMRRG
                     KVVGVLERDEADEEILARLMIGKTIEPPRIEIHERGEEILRLEDIKLSPRLKGINLKL
                     YRGEILGLAGVEGNGQRELLEVITGLLAPDSGKVFYKDKEIERLDPRSVREIGIGFIP
                     EDRQMTGLILDFSVAENIILGRHYKEPYTVNGRLNVPVIRDIANKIVNTYDIRTSSID
                     KYVRFLSGGNQQKVILGRELWSNPELLIASQPTRGLDIGATEMIHNILLSARKNGMAI
                     ILHSSDLDEIISLSDRIAVIYNGEIVSELSPKGIDETILG"
BASE COUNT         1020 a          469 c          851 g          856 t
ORIGIN      
        1 atgaattgtt cagacaggaa ggggtaagga atatacaggg ctataacttg aagaagaagg
       61 actctccgct gtattatata gtcgttattg tggatgaact ggcagacctt atgatggtgg
      121 ctcctaaaga gattgaaaga cttatatgca gattagccca gatggccagg gctaccggga
      181 tacatcttat catagcaacg cagagacctt cggtggatgt gattaccggc ttgataaagg
      241 ctaatttccc ttcaaggata gcatttgcag tatcttctca ggcagattca cgtgttatac
      301 ttgatatagg tggagcagag aaacttttag gaaaagggga tatgctatat tctcccatat
      361 gggctatgag acctgtaaga gttcaggggg cgtatgtctc tgatgaagag atagaaaaag
      421 tattgaactt ctggaaattg cagggaacac caaattatgc agttactcag gaggagcttg
      481 agagtgagga tattatctct agcagtgatt cagagagtaa tgatgagtat ctggaagagg
      541 ctaagaagat actcaaacag aagggtggca tatctaccag cttattacag aggatgctca
      601 gaattggcta tggcagggcg gcaaggataa ttgaccttct ggaagctgaa ggttatgttg
      661 agaggggaga agacggaagg gtaagggtag ttagattaga ttgagataaa aaataatttc
      721 tacaggaggg atcaataatg aaaaagtttt tagtggggat cttggtttta actctggtat
      781 ttgggttcag ttcattcagt tatgcagcta aagcaaaggt agggattgtg tatgatgttg
      841 gaggaagagg cgataaaagt tttaacgacg ctgcatacaa gggacttcaa gatgcagtaa
      901 agaaacttga tatagagggc aaggatgtgg aaccaggaga aggtggagct aacagggaag
      961 agttacttag attgttagca gaacagggat atgacctggt aataggtgta ggatttctct
     1021 ttacagatgc ccttgtaaag gtagccaaag actttccaaa caccaagttt gctatcgtag
     1081 acggggtaat agaagacctc cccaatgtcc gatctcttgt atttaaagag catgaagggt
     1141 cattcctggt tggagctata gctggtctta tgaccaagac aaataaagtt ggatttgttg
     1201 gtgggatgga tatgtcactc atccataaat ttgaggttgg atatagagct ggggtaaaat
     1261 atgtaaatcc aaaggcagag atttttgtaa actatatagg tgttaccggt gatgccttta
     1321 aggacccggt gaagggtaag gagttagcat tatcccagtt caagcagggt gcagatattg
     1381 tttatcatgc atcaggtgcc tctggagaag gtgttataga agcagcaaaa gagatgaaaa
     1441 aattcgctat aggtgtggac tctaatcaaa actggattgc tccgggatat gttcttacca
     1501 gcatgttaaa gaaggttgat gtagcagtgt ataaaactat cgaagacctg gttaatggca
     1561 agtttactgg tggtgtaaag gtttatggtc ttagagagaa tggggttggg gttgcatatg
     1621 acaagtataa cgagaagctc ataccagaat ctgttcgaag aagggtggct cagataaggt
     1681 tggatataat cgcaggtaag ataaaggttc ctgaaaccaa gtagagatgt cagaagagtt
     1741 tatactggaa acagaaggta taacaaagca tttccctgga gtcctggcta acgacgatgt
     1801 gagtattcag attagagaag gagagataca ttctatcata ggagagaacg gggcggggaa
     1861 gaccacgttg atgaatataa tctatgggat tttccgcccc gacaggggta agatattcta
     1921 taaggggaga gaggttgaga ttaataaccc ttccacagct ataagcctgg ggatagggat
     1981 ggtgcatcag cattttatgc ttgttccacc gtttaccgtc tgggaaaata taatcctggg
     2041 cagggaagtt ttaaagagag gagtcctcga taaagactct gtatctgaag acctgaataa
     2101 acttatgagt gattacaggc tttcagtccc tctcgaagaa aaaattgaag acctgccggt
     2161 aggtatatgc cagaaagtag aactgataaa gcttttatat agaggagcgg aggtatttat
     2221 tttagatgaa cccaccgctt tacttgtccc tcaggagatt gaagaacttt ttgaaatact
     2281 aaataattta aagcggcagg ggaaaacgat tatttttatc tctcataaac tcaaagaggt
     2341 gctcaggatt tctgatagaa ttactgttat gagaagaggt aaggtggtag gtgtactaga
     2401 aagagatgag gcagatgagg agatactggc tcgacttatg ataggcaaga ctatcgaacc
     2461 acccaggatt gagatacacg agaggggaga ggagatatta agacttgagg atataaagtt
     2521 atctcccaga ttgaagggta tcaatctcaa gttataccgg ggtgagatac ttggtttagc
     2581 tggtgtggag ggtaatggac agagagagct attagaggtg attacagggc tattagctcc
     2641 tgattctggg aaggtcttct ataaagacaa ggagatagaa cgtctggacc ccaggagtgt
     2701 aagggagatt ggcataggat ttattccaga agatagacag atgactgggc ttatacttga
     2761 tttttcggta gctgagaata ttatccttgg cagacattac aaagagccat atactgtgaa
     2821 tggtagactg aatgtacctg taattaggga tatcgccaat aagattgtaa atacgtatga
     2881 cataaggacc tcttctatag ataagtatgt caggttcctc tctggaggaa accagcagaa
     2941 ggttattctt ggtagagaac tctggtcaaa tccagagtta ctcattgcct cacaaccaac
     3001 cagaggatta gatataggtg ctactgagat gatacataat atccttttgt cagcacgtaa
     3061 gaatgggatg gctataattt tacattcttc tgatctggat gaaataatat cactgagtga
     3121 tagaatagca gtaatatata atggagaaat agtgagtgaa ttatctccca aagggataga
     3181 tgaaacaatc ctgggt
//
LOCUS       DAOTKU010000159         3189 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_25052_length_3189_cov_3.739949, whole genome shotgun sequence.
ACCESSION   DAOTKU010000159 DAOTKU010000000
VERSION     DAOTKU010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3189
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25052_length_3189_cov_3.739949"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..1228)
                     /locus_tag="P5310_04725"
     CDS             complement(23..1228)
                     /locus_tag="P5310_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32697.1"
                     /translation="MYNPHREHKETVEIEGTIPLQAFDCKGKGILTVNLMEDGKICSI
                     FDVTLPSYGYTTIFFREKEKVDEANWEIGDSIENSNITLARRDKRVVLKTAWRDYELS
                     LQPFMIKEVLNGTLISRSPIKGGDILSWIRMVPYPQLKIAMRLDWNIMFVETFSILDD
                     VVLCNMEFEFLRPTLIGEGDWNPNGLIFGVQSKNKGTIYYDIPFGVVAHQNPKESFIP
                     ALRFATLQDEESGVAIISKSGNHSFKVSGDNGFIGIGLGASTLGGPARPALLDVDRAK
                     SSVLHYNNWEYEYFNGKYQSEFFIYPYRGDWQDRDIPHISRQKTESDYLVDISKRTGV
                     SNIDPQKSFIEIQPDKGVEISTLELQGDKLFVRLYETSGRGTDFILSVGGKVLKGEIS
                     PFGIFEGWI"
     gene            complement(1465..2442)
                     /locus_tag="P5310_04730"
     CDS             complement(1465..2442)
                     /locus_tag="P5310_04730"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32698.1"
                     /translation="MKTVYFSLLYHGNMNYDRYPKRVIRKYFPEIYEIVIDAMKKYHP
                     LKLNLQISGMSIESLKICAPYVLEDFRMLAREGRLIFTGTYYSEPVNVTTDGETDFRC
                     AWLGTEIVRRNLGEPYGFFPQEQSYHPQIPWIVKSLGLTWVTVPGNPKIVNPFVIVGL
                     DGTKIYGIPTFGMNHNNLEYYFDISDDNGLIVGAGDLEIPHNFDLLMSKIEELNNNGK
                     KLEIVTIPEYLEKFQPEKEIFLPPCAWQKENVLENPSFSRWISDPQDIIVHKATMKAM
                     SDTESATVFNTIWNLHNNSNCDIEVENAKTVVPLDPIMADIESAEELQI"
     gene            complement(2469..>3189)
                     /locus_tag="P5310_04735"
     CDS             complement(2469..>3189)
                     /locus_tag="P5310_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32699.1"
                     /translation="AHFIVDYAIEEAKKTNFNSRITIPVVPFYHTYYNIDSTGLIWRD
                     LNGKVLSFQGSYIEIETDDEVEEIGYILNFDYTKVHSEHWLKSGYPGKRVSPMIISKT
                     IDRGKSIYCATDFEIYSLEYGDPESIEVLTNLVKYVGGEEIELVKIKAPGTVEVNSYV
                     SENSLVIFLLNHTTNQLTTGKAVRYIIPIKDITIEVNWNKRLESAFCLSKSNLDLKRE
                     ESKIILTLPYLEEYDVIVLKE"
BASE COUNT          948 a          685 c          468 g         1088 t
ORIGIN      
        1 gtttttctta attctaaata gcctatatcc atccctcaaa tataccaaaa ggagatatct
       61 ctcctttaag taccttcccg cctacgctta atataaagtc tgttccccta cctgaggttt
      121 catataatct cacaaagagc ttatccccct gtaactctaa ggtagatatc tctacccctt
      181 tatcaggttg aatctctatg aatgacttct gaggatcgat attacttact ccagtcctct
      241 tggatatatc aactagataa tcagattcag tcttctgcct tgatatatga ggtatatccc
      301 tatcttgcca atcacctcta tatgggtata taaagaactc agattgatac ttaccattaa
      361 aatactcata ctcccaatta ttataatgta agacactact tttagcccta tcaacatcca
      421 aaagtgcagg acgagctggt ccccctaaag ttgatgctcc taaccctatc cctataaaac
      481 cattatcacc agaaacctta aaagagtggt ttccactctt agaaattatt gcaactccac
      541 tttcttcgtc ttgaagagtc gcaaatctta atgcaggaat aaaggattcc tttggattct
      601 ggtgtgcaac taccccaaaa ggaatgtcat agtatatggt acccttattc ttactctgca
      661 ctccgaagat taaaccattt ggattccaat ctccttcccc gataagtgta ggtctcagga
      721 attcaaattc catattgcac aatacaacat catctaagat ggaaaaagtt tctacaaaca
      781 ttatattcca atccaatctc atggcaattt ttagctgagg ataggggacc attcttatcc
      841 aacttaaaat atccccacct tttataggtg acctggagat aagggtccca tttaatactt
      901 cctttatcat aaacggctgc agtgaaagct cataatctct ccaggctgtc tttaaaacta
      961 ctcttttatc tcttctagct aatgtaatat ttgaattctc aatactatct ccaatctccc
     1021 agttcgcttc atctactttc tctttctctc tgaagaagat agtagtatat ccgtaggaag
     1081 ggagtgttac atcgaaaatc gagcatatct ttccatcctc catcaaattt acagtcagta
     1141 tcccctttcc tttacaatca aatgcttgta gtgggattgt accttctatc tcgacagtct
     1201 ctttatgctc tctatgaggg ttatacatta ttaagcctct agtataatcg tctggaactg
     1261 ttaggtaaga agagatctct cctagaatgg tatttattac ctctctagag aatagacttg
     1321 ccttattaaa cgaaaggatc ctttctctcc tcttttctat aaggggatac cagcctgtag
     1381 catcactatt tactccccat aaaactaaat gccaactttt agataaggcg gttaccttac
     1441 ctcccctggc taaatattct tcttctatat ttgtaattcc tctgcagatt ctatatccgc
     1501 cataattgga tccagaggaa ctactgtttt tgcattttca acttctatgt cacagttact
     1561 attattatgt agattccata tagtattaaa tactgtagca ctttcagtat ctgacatcgc
     1621 cttcatcgtt gctttatgta caattatatc ctgaggatca gatatccatc ttgaaaagga
     1681 tggattctcg agcacatttt ccttttgcca tgcacatgga ggcaaaaaga tttctttttc
     1741 aggctgaaat ttctctagat attccgggat agtaactatc tctagctttt tcccattatt
     1801 atttaattcc tctatctttg acataagaag atcgaaatta tgtggtattt caaggtctcc
     1861 agctcctaca ataagtccat tatcatcaga gatatcaaag taatattcta gattattatg
     1921 attcatccca aaggtcggaa taccatatat ttttgtacca tcaagtccaa ctatcacaaa
     1981 aggattgaca attttggggt tccctgggac agtaacccag gtaagtccaa ggcttttaac
     2041 tatccatgga atttgagggt gataagattg ttcttgaggg aaaaaaccat aaggttcacc
     2101 tagatttctt ctcacaattt cagttcccag ccaggcacat cgaaaatcag tttcgccatc
     2161 cgtagtaaca tttacaggtt ctgaatagta agtccctgta aaaataagcc ttccttctct
     2221 ggctaacatc ctgaaatctt caagtacata tggagcacat attttaagag actctatact
     2281 cataccagaa atctgtagat taagcttgag aggatggtat tttttcatag catcgattac
     2341 aatctcataa atctctggga aatattttct tattacccgt ttagggtacc tatcatagtt
     2401 catattccca tgatataaaa ggctaaaata taccgtcttc atagtcaaaa acctcctaat
     2461 actgtattct attcttttag tacaataaca tcatactctt caaggtaagg aagagttagg
     2521 ataattttac tttcttctcg tttcaaatct aagttacttt ttgaaagaca gaaagctgat
     2581 tctaatcgtt tattccagtt tacctctatc gttatatcct taattggtat tatatatcgc
     2641 accgctttac cggtagttaa ttggtttgtt gtatgattaa gtaaaaatat caccaggcta
     2701 ttctcagaaa cataagaatt aacttccact gttccaggag ctttgatttt tactaattct
     2761 atctcctctc ctcctacgta tttgactaga ttagtcagaa cttctataga ttctggatct
     2821 ccgtattcta aagaatagat ttcaaaatct gtagcacagt atatactttt acctctgtct
     2881 attgttttag agataatcat agggctaact ctttttccag gataaccaga cttcagccag
     2941 tgttcactgt gcacctttgt ataatcaaaa tttaggatat accctatttc ttctacttca
     3001 tcatctgtct ctatctctat atagcttccc tggaaagaaa gcaccttacc atttaaatct
     3061 ctccatataa gaccagtaga atcaatatta tagtatgtat gataaaaggg aacaacaggt
     3121 atcgtgatgc gtgagttgaa gtttgtcttc ttagcctctt ctatggcgta atccactata
     3181 aaatgagcc
//
LOCUS       DAOTKU010000160         3177 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_25167_length_3177_cov_3.444266, whole genome shotgun sequence.
ACCESSION   DAOTKU010000160 DAOTKU010000000
VERSION     DAOTKU010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25167_length_3177_cov_3.444266"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            37..2052
                     /locus_tag="P5310_04740"
     CDS             37..2052
                     /locus_tag="P5310_04740"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HRU32700.1"
                     /translation="MRETLTVKQCSSVANQFGKKRLLSELYGCSGWEFTFEGQKWVGD
                     WQYVLGVNMRCQHLTLYSLRGCRKRDYPPSFNYNTTWWKYNKIVEDYFARLSLILSEG
                     KVVRDILLIHPIESAWMVFDGSNSEEVSKIGDEFQGIADTLLALHRDFDLGDESIIEG
                     YGKIVNGLFTINQASYKLVVLPPMKTIRGKTVNLLKEFLDSGGNVIVLKPYPEMVLAQ
                     EEDLSWLFEHPNVSILNDKRELSSKLEELLKPRIRIQETPGEEDGSLLYMEREIEGKD
                     VFFIVNTDRNNPHTVEIIIENNGKVEEWDALTGEIREVNSIQGEGVTRIKTTFGPAES
                     RLYVIDPHSRPAAEEPAELHYKRGLYIGPVTEFTRSNPNVLTLDFCQYRFKGDKWSDI
                     KPVWKAQLEIRRKLGMRDIHVNGIEQRWRWIYQPHPNDNTPVEFRFTFNVRDIPKTPV
                     SLVVENAEDFGIYLNGEAISNKPDGWYLDRSFDRIKLPALKKGENEIILACLYKNRME
                     VEDCYIIGDFGVDILTREIIKEPDRLHFGDWCMQGYPHYAGSISYREELDIDLEDNER
                     ILIKIGKHSAITTAIWINGKLAGYIPWKCADGLDVTPYMVKGKNLIEIEVVASPRNML
                     GPLHQKSGKRPWTDSRSFRTEGDEFTPDYVLMPFGLFDQVKLEVFSK"
     gene            complement(2112..>3177)
                     /locus_tag="P5310_04745"
     CDS             complement(2112..>3177)
                     /locus_tag="P5310_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32701.1"
                     /translation="HTHTGPVTTGLLAGEIDSTYIEVLVKKIASSIAMAKKNLKEASV
                     RAGRGKEQNLVFNRRYVMKDGWVKTNPGKLNPDIIKPAGPVDPDILVILLEDNTKSPI
                     ALLVNYANHVDTIGGTGISADYPGILSNTIKRFLGDIPVLFLTGAEGDINHIDVMDSN
                     PQGGYEEAKRIGITLAGEVLKILVKLSLINGRLGIKSSFLNVPVRKPDQVEIDNAKKL
                     LEETVEISPGELTAFDLAKGSLDVERVYAQEVIALSQLDRDFEELELQAISLGELVFL
                     GVPGEPFAEIGLAIKRESPFTYTGIVSLASGYSGYIPTEKAFNEGGYEIRLARSSKLD
                     RATEKTIIREGITLINSIYK"
BASE COUNT          976 a          626 c          686 g          889 t
ORIGIN      
        1 tagggagttc ccggaataga tatacttact gagtctatta gagagacatt aacggtaaaa
       61 cagtgcagca gtgttgccaa tcagttcggg aagaagagat tactatcaga attatacgga
      121 tgctcaggat gggagtttac gtttgaagga cagaagtggg ttggtgactg gcagtatgta
      181 ctgggtgtaa acatgaggtg tcagcatcta acactttata gcctaagagg atgcagaaaa
      241 cgagactatc caccttcatt taactataat acaacctggt ggaagtataa taagatagtg
      301 gaagattact tcgccagact ctctctcata ctctcagagg gaaaggttgt gagagatata
      361 ctccttatac atcctataga aagtgcatgg atggtatttg atggatcaaa ctctgaagag
      421 gtttcaaaaa taggagatga gtttcagggt atcgcagata cactcctggc tcttcacagg
      481 gactttgacc tgggagatga atccatcatt gagggatacg gaaaaattgt aaacggacta
      541 ttcaccatta accaggccag ctacaagctg gtagtcctcc cacctatgaa gacgataaga
      601 ggaaaaacgg taaatcttct caaggaattc ttagatagtg gggggaatgt tatagtcctt
      661 aagccttacc cggagatggt tttggctcag gaagaagacc tgtcctggct atttgaacat
      721 ccaaatgtat cgatactgaa tgataaaaga gagttgtcca gtaaattgga agaactctta
      781 aaacccagga tcaggataca agagacacca ggagaagagg atggttctct cttatatatg
      841 gagagggaga tagaaggtaa ggatgtattc tttatagtaa atacagacag gaacaatccc
      901 catacagttg aaataataat agagaataac ggtaaagtcg aggaatggga tgcccttaca
      961 ggtgagataa gagaagtgaa cagcatacag ggagaagggg tgaccaggat aaagaccacc
     1021 ttcggacctg cagaatcaag gctatacgtg attgacccac attccagacc agcagctgaa
     1081 gagccagcag aactccacta taagagaggt ctttatatag gaccggtgac cgagtttacc
     1141 agaagtaatc cgaatgttct taccctggac ttctgtcagt atagatttaa aggagataaa
     1201 tggtctgata taaagccagt atggaaggct cagctggaaa tcagaagaaa acttgggatg
     1261 agagacatac atgtaaatgg gatagagcag agatggagat ggatttatca gcctcatcca
     1321 aatgataata ctcctgtaga gttcagattt acattcaatg taagagacat ccctaaaaca
     1381 ccggtaagcc ttgtagtcga aaatgcagag gactttggga tatatctcaa tggagaagct
     1441 atatctaaca aacctgatgg ctggtatctg gatagaagtt tcgaccggat aaaactacca
     1501 gcattaaaga aaggcgagaa cgagataata cttgcctgtc tctataagaa caggatggaa
     1561 gtggaggatt gttatataat aggggatttt ggggtagata tactgaccag ggagatcata
     1621 aaagaacctg atagacttca ttttggtgac tggtgcatgc agggataccc gcactatgcg
     1681 gggagtatct cctacagaga agagctggat atagatctag aagataacga acgtatcctt
     1741 ataaaaattg gtaaacattc tgcaataact actgccatct ggataaacgg taaacttgct
     1801 ggatatatcc cctggaaatg tgctgatgga cttgatgtta caccttatat ggtgaaaggg
     1861 aagaatttga tagagataga ggttgttgcc agcccgagaa atatgcttgg tccacttcat
     1921 caaaagagcg gtaaaagacc ctggacagat tccagatcct tccgaacaga aggggatgaa
     1981 tttacaccgg attacgtgct gatgccattt ggactctttg accaggtaaa actcgaggtc
     2041 ttctcaaagt aaaaataagg aggcggaatt ccgcctcctc tactacttga aaaacagctc
     2101 tattcctaga attatttata aatactatta atcagtgtta tcccttctct tattatagtt
     2161 ttttctgtcg ctctatccag tttactgctc ctggcaagcc ttatctcata tcctccctca
     2221 ttaaatgctt tttctgtcgg tatataacct gagtatccac tagctagaga tactatacct
     2281 gtataggtga aaggactttc tctctttatt gccagtccta tctctgcaaa tggttctccc
     2341 ggtacaccta agaataccaa ttcacccagg gagattgcct ggagctcaag ttcttcaaaa
     2401 tctctatcca gctgtgaaag agctataacc tcctgggcat agactctttc tacatctaag
     2461 cttccctttg ccagatcaaa ggcagtgagt tctccaggag atatctcaac cgtctcttct
     2521 aaaagttttt tagcattatc gatctctacc tgatctggct ttcttactgg cacattcaga
     2581 aaagagcttt ttattcccag acgcccattt attagactta gttttaccag aatttttaac
     2641 acttcacctg ccagtgttat tccaattctt tttgcctctt catatcctcc ctgaggattt
     2701 gaatccatca catctatatg gttaatatcc ccttctgctc cggtaagaaa aagcacagga
     2761 atatctccaa gaaatctctt tatggtgttg gaaagtatac cgggataatc ggcagatatc
     2821 cctgtacctc ctattgtatc cacatgattg gcataattta cgagtaaagc tattgggctt
     2881 ttcgtattgt cttctaatag tatcaccagg atatcagggt ctacaggacc agcaggtttt
     2941 ataatatctg gattaagctt ccctgggttt gtctttaccc aaccatcttt cataacatat
     3001 ctgcgattaa atactaggtt ctgttcttta ccccttccag ctcttacact ggcttccttc
     3061 agattcttct tagccatagc aatactgctt gcaatctttt ttaccagcac ctctatatag
     3121 gtagaatcta tctctcctgc cagaagtcct gtggtaacag gtccagtatg agtatgg
//
LOCUS       DAOTKU010000161         3176 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_25176_length_3176_cov_3.343159, whole genome shotgun sequence.
ACCESSION   DAOTKU010000161 DAOTKU010000000
VERSION     DAOTKU010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3176
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25176_length_3176_cov_3.343159"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="P5310_04750"
     CDS             complement(<1..533)
                     /locus_tag="P5310_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013433557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRU32702.1"
                     /translation="MKTLYESDFLVIRDETRCIRCKVCVRQCANEVHQYDEEDDRIIS
                     DNSKCVGCHRCVTMCPTKAITIMRNPLEFRENYQWTERTIKDIYKQSETGGILLTGMG
                     NDKNYPVYWDRLLINASQVTNPSIDPLREPVELKTFIGRKPERLDFDSYGNLLTEMPP
                     QLELEIPIMFSAMSFGS"
     gene            complement(530..1642)
                     /locus_tag="P5310_04755"
     CDS             complement(530..1642)
                     /locus_tag="P5310_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015566556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase family protein"
                     /protein_id="HRU32703.1"
                     /translation="MRDINIKYIEGQIRIPSACGLLGYINTRGELTSGGHIVDAMSVM
                     RERGNGLGAGFAGYGIYPDREDYYAFHIFFEDPKAREETSSLLYNYLDVVQEEKIPTR
                     KVKNIRNAPMIWRYFVKPKEEYLEEDVSEDDFIVDLVMKINSEIKGAYVFSSGKNMGV
                     FKGVGYPEDIGEFYKLEEYNGYSWTAHSRFPTNTPGWWGGAHPFSLLDWTVVHNGEIS
                     SYGTNVRYIEMFGYKCTLMTDTEVITYIMDLLIRRHRLPVDIVVKALAPPFYSTVERL
                     DEEEKNKIKAIRAVYQSCLLNGPFSIIVGFKNGLISLTDRIKLRPLVAGKSGDFVYMA
                     SEESAIRELSPKLDRVWMPRGGEAVISMLEEKRILV"
     gene            complement(1833..>3176)
                     /locus_tag="P5310_04760"
     CDS             complement(1833..>3176)
                     /locus_tag="P5310_04760"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HRU32704.1"
                     /translation="LFFYEGKLLARQDKTLLPNYDVFDEVRYFKPARSTHPVVFNGKR
                     LGITICEDIWNDKDYWDRRLYDVDPIEDLISQGIDIIINISASPYHYSKRSLRIDMLS
                     YIAKKYKKIAVYVNQVGGNDELIFDGSSLVIDEGGCVIWEGKNFEEDFYVIDTEDIPA
                     GKESNHLKEDISFVHKALISGIRDYLAKTGFSRALVALSGGIDSSVVACLAVSALGAE
                     NVLGVGMPSRYSSKESLIDAEILAKNLDIEFRVIPIEKVFSTFLETLNSEAHPVMDIA
                     EENLQARIRGNIIMFISNRKGYLVLSTGNKSELAMGYCTLYGDMSGGLSVIGDVPKTM
                     IYELARFINSEEEIIPNRILLKPPSAELRPDQKDEDSLPPYSILDPIIKAYVEDNLSV
                     DSMVSMGFDQGLVRDVVRIIDSAEYKRRQSAPILKVTTKAFGTGRRMPIVWRRNW"
BASE COUNT          897 a          743 c          510 g         1026 t
ORIGIN      
        1 atcgaaccaa aagacatagc tgaaaacatt attgggattt cgagctccag ctgtggaggc
       61 atctctgtaa gtagattccc atagctatca aaatccagtc tttcaggttt tctccctata
      121 aaagtcttaa gttccaccgg ttctcttaaa gggtctatag agggatttgt cacctgactg
      181 gcatttatga gaagcctgtc ccagtataca ggataattct tatcattacc catcccggta
      241 agaagaatac cgccagtttc agattgctta tatatatcct tgattgttct ctctgtccac
      301 tgataatttt ctctgaattc aagtggattc ctcattattg ttatagcctt tgtggggcac
      361 atggtaacac atctatgaca gccaacacac ttagagttat cactgataat cctgtcatct
      421 tcctcatcat actgatgcac ctcgttagca cattgtctta cacagacctt gcatctgatg
      481 cacctggtct catctcttat tacaagaaaa tctgattcat atagagtctt cataccagta
      541 ttctcttttc ctccaacatt gatataaccg cctctcctcc tcttggcatc cataccctgt
      601 caagtttcgg agataactct cttatcgcag attcctcaga agccatataa acgaagtctc
      661 cactttttcc agccaccagg ggtctcagtt ttatcctgtc ggtaagtgat ataagaccat
      721 ttttgaatcc aacaattata gagaatggtc cgttcaataa acaactctga tataccgccc
      781 ttatagcctt tatcttgttt ttctcttctt cgtcaagtct ttctacagta ctgtaaaatg
      841 gtggagccag ggctttaaca actatatcta ctggaagtct gtgcctcctg ataagcaggt
      901 ccataatata ggttatgacc tctgtatcgg tcataagagt acacttataa ccaaacatct
      961 caatatatct gacgtttgtt ccataagagg atatctctcc attatggacc accgtccaat
     1021 ctaagagaga aaaaggatgt gcacctcccc accatcccgg ggtattggtg ggaaacctgg
     1081 aatgggctgt ccagctgtac ccattgtact cttcaagttt atagaactct cctatatctt
     1141 cgggataacc gacaccttta aagaccccca tattcttacc actggaaaag acataagccc
     1201 cttttatctc actgttaatc ttcataacaa gatcaacgat aaagtcatct tctgatacgt
     1261 cttcttccag atattcctct ttgggtttaa cgaaatatct ccagatcata ggggcatttc
     1321 tgatattttt tacttttctt gtgggaattt tttcttcctg taccacatcg aggtaattgt
     1381 acagaagaga agatgtctct tctctggctt ttgggtcttc gaaaaagata tgaaaggcat
     1441 aataatcttc tctatcgggg tatattccat aaccagcaaa gcctgcgcca agcccattac
     1501 ccctttctct catcacactc atagcgtcta caatatgtcc tccagaggtc agttctcccc
     1561 tggtgtttat atagcccaaa agtccacagg ctgaaggtat tctgatctgc ccctcgatat
     1621 acttaatatt tatatccctc atatctgttt cctccttcta attgggatag aataatacaa
     1681 taaagctatt cccccattgg gatataacgt tgcctgcttc ggcagaacag acattgtcct
     1741 tataagactc cttcaagaca cctttgtctt aaaggcagaa gagattgtaa aataattata
     1801 ttgaaaaaaa cttccctgtc aagagcaggg gactaccagt tccttctcca tactatcggc
     1861 attcttcttc cagttccaaa agccttggta gttaccttaa ggattggggc agactgtcgt
     1921 ctcttgtact ctgcagaatc aattattctt actacatccc ttaccaatcc ttgatcaaaa
     1981 cccatactaa ccatactatc aacagaaagg ttatcctcta cataggcttt tataatgggg
     2041 tcaagaatac tgtatggagg taatgaatcc tcatctttct ggtctggtct cagttctgca
     2101 gatggaggtt tcaaaagtat cctgtttggg attatttctt cttcactatt tataaatctt
     2161 gcaagttcat atatcattgt tttaggaacg tcacctatta ccgaaagtcc cccagacata
     2221 tctccataga gagtacagta acccattgca agttcagatt tatttccagt actcaataca
     2281 agatatccct ttctattgga gataaacatt ataatgttac ctctaattct ggcctgaagg
     2341 ttctcctccg ctatatccat aacaggatga gcttcagagt ttaatgtttc tagaaatgtc
     2401 gaaaagacct tctcaattgg tatcactcta aactctatat ctagattttt agctaagatt
     2461 tctgcatcta taagactttc tttagatgag tatcttgatg gcattcctac acccaagaca
     2521 ttttcagccc ctaatgcaga aacagccaaa caggcaacta cagatgagtc tatgccacca
     2581 cttaaagcta caagggcacg actgaaacct gtcttagcaa gataatctct tatccctgaa
     2641 atcaacgctt tatggacaaa tgatatgtcc tcttttagat gattcgattc tttccccgcg
     2701 gggatatcct ctgtatctat gacataaaaa tcttcttcaa agtttttgcc ctcccatatt
     2761 acgcatcccc cctcatctat caccaggctt gagccgtcaa agataagttc gtcgttacca
     2821 ccgacctggt ttacgtatac agcaatcttt ttatattttt tagctatata actcagcata
     2881 tctattctga gacttctttt actataatga tatggagaag cagaaatgtt tatgattata
     2941 tctatacctt gagatataag atcctctata gggtcaacat catacaatct tctatcccag
     3001 tagtccttat cattccatat atcctcacag attgttatcc ctaatctttt cccattgaag
     3061 actacaggat gggtagatct ggcaggttta aaatatctta cctcatcaaa gacatcataa
     3121 tttggcagta atgttttatc ctgtctagcc agcagtttac cttcatagaa gaacag
//
LOCUS       DAOTKU010000162         3164 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_25288_length_3164_cov_2.958186, whole genome shotgun sequence.
ACCESSION   DAOTKU010000162 DAOTKU010000000
VERSION     DAOTKU010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25288_length_3164_cov_2.958186"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /locus_tag="P5310_04765"
     CDS             complement(<1..665)
                     /locus_tag="P5310_04765"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HRU32705.1"
                     /translation="MNITTQYTMLDIAPTVSRILQVRPPANSSGAVIEEIQSKLGVTD
                     RVGLVVIDAFGVSTWQRYKHLTSNFNLLADKNLVYIKSVLPAKTPVNFATMVTGAEPE
                     VHNIKSRTDTLKVETIFHVLSEEKMKSAAIGWEASTVGILLSPLADYKGIASSNTDEE
                     VVKLATEIAKENRPNFLILQLLEVDSTGHKFGLEGEEIKEAILSADKHLGQILSTLLE
                     ERYA"
     gene            838..2196
                     /locus_tag="P5310_04770"
     CDS             838..2196
                     /locus_tag="P5310_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HRU32706.1"
                     /translation="MERDKKITLSESEIPQRWYNILPDLPEPLPPPMSPATKEPLKPE
                     ELAKIFPMELIKQELSQERWIEIPDELLKVYRIWRPTPLTRAYELEKALKTPARIYYK
                     NESVSPPGSHKPNTAVAQAYFNKKAGIKKLTTETGAGQWGSALAFACNIFGLECTVYM
                     VRVSYEQKPYRKVMMQVWGATVHPSPTNLTNSGRTILQEDPNSPGSLGVAISEAVEDA
                     VLHDDTNYSLGSVLNHVILHQTIIGLEAEKQLEIAGEKKPDIVIGCVGGGSNFGGICT
                     PFVRRRLQGEDIRFIAVEPSSCPRLTKGQYRYDYGDVAGLTPLLMMYTLGHTFMPPSL
                     HAGGLRYHGMAPIISLLARKGIIEAKSYNQNPVFESAVLFAKTEGIIPAPETAHAIKA
                     VVDEAIRCRETGEEKCILFNFSGHGHFDLSAYEAYLSGKLIDYEYPEEKILEALRDLP
                     VI"
     gene            2317..>3164
                     /locus_tag="P5310_04775"
     CDS             2317..>3164
                     /locus_tag="P5310_04775"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HRU32707.1"
                     /translation="MKKEFLMIPGPTPVPDSVLRKMSVPMVNHRGKEFAQILKNVTEK
                     MKKVYLTEGDVILLTSSGSGGMESAVVNFLSPGDKAIFCVSGTFGDRWSKIAQVYGAN
                     VIRVEVPPGEGVTLDKIEDALKKNPGVKAVYITHNETSTSITNDLSQVGKLAHNYGAI
                     AVVDAVSSLGAIELRMDEWQLDVVVTASQKALLTPPGIAFAAVNNRAWELNKSAKMPR
                     FYFDLGEAKKMAANGETPFTPAVSQVFAIDTALDILLSEGLENSWKRHRLLGKATREG
                     VKALGL"
BASE COUNT          949 a          582 c          744 g          889 t
ORIGIN      
        1 gcatatcttt cctctaaaag agtggataag atctgtccaa gatgtttatc cgccgataat
       61 atcgcttctt tgatctcttc gccttcgagc ccaaatttat gccctgtaga atcaacctca
      121 agcagctgga gtataagaaa attgggacgg ttttcttttg ctatctctgt agccaatttg
      181 acaacctctt catctgtatt ggatgatgct atacccttat aatctgctaa tggtgaaagg
      241 agtataccga cagtacttgc ctcccaccct atagcagcag acttcatctt ttcctctgat
      301 agtacgtgaa aaatggtctc aactttcagg gtatctgtac gactctttat attgtgtacc
      361 tctggttctg cacctgtaac catggtagca aaatttacag gggtctttgc tggcagaaca
      421 gatttgatat agaccagatt tttatcagct aagaggttaa aattggatgt gaggtgttta
      481 tatctttgcc aggtggagac accaaaggca tcgataacta ccaagcccac cctatcggta
      541 acgcccagct ttgattgtat ttcttctata actgcaccag aactattggc tggcggtctt
      601 acctgtaaaa tcctgcttac tgtaggagct atatctaaca ttgtatattg agttgtaata
      661 ttcatcttct gtctattcac ctccagtgtg acttttgaag ttctaaagga tattataaca
      721 cctgaggttc agaaaagttg aatgggctaa cgtgttgtgt tataattatg caataatttt
      781 ctcttagaaa ggagaaaaag atgagaagag aagagttttt aagtaaggag gagaaagatg
      841 gaaagagata agaagatcac cttatctgag agtgagattc cacaaaggtg gtataatatc
      901 ttacccgatt taccagagcc attaccacct ccaatgagtc cagctacaaa agagcctttg
      961 aaacctgagg agctggcaaa aattttccct atggaactga taaagcaaga gctcagtcag
     1021 gagaggtgga tagaaattcc ggatgaactt ctaaaggtat acaggatatg gagacctacc
     1081 cctcttacca gggcttatga gctggagaag gcattaaaga cgccagccag aatttattat
     1141 aaaaatgaga gcgtaagtcc ccctggaagc cataaaccaa ataccgctgt tgcacaggca
     1201 tattttaata agaaagcagg gataaaaaaa cttacaactg agaccggtgc tggacagtgg
     1261 ggaagtgccc tggcttttgc ctgcaatata tttgggttag agtgcaccgt ctatatggtc
     1321 agggtcagct acgaacaaaa accttacaga aaggttatga tgcaggtatg gggagctact
     1381 gtgcatccat cacctaccaa tcttaccaat tctggaagga ctatactgca ggaagacccc
     1441 aattctcctg gaagcctggg agtagcaata tcagaagcgg tagaagatgc agttctgcat
     1501 gatgatacta actactctct gggaagtgtt ttaaatcatg ttatacttca ccagacaatt
     1561 atagggttgg aagcagagaa acagttggag atagcgggag agaaaaaacc tgatatagtt
     1621 attggatgtg tgggtggcgg aagtaatttc ggagggatat gtacaccatt tgtaaggaga
     1681 agattacagg gtgaagatat aagatttata gcggtggaac catcatcctg cccaagatta
     1741 acaaaggggc agtacagata tgattatgga gacgttgccg gtcttacccc attattgatg
     1801 atgtatacac taggacatac atttatgcca cccagtctgc atgctggagg tttgcgttat
     1861 catggaatgg ctccgataat atctctactt gccaggaagg ggataataga ggctaaatct
     1921 tacaatcaga atcctgtatt tgagtctgcc gttttattcg ccaagacaga agggatcatc
     1981 cctgctccag agacagctca tgctataaag gctgtggtgg atgaagctat caggtgcagg
     2041 gagactgggg aagaaaaatg tatcctcttt aacttcagcg ggcatggaca ttttgacctc
     2101 tctgcctatg aggcttatct gagcggaaag ctgatcgatt atgagtatcc agaagaaaaa
     2161 atcctggagg cattaaggga tttacctgta atttaatcct gatatagggg gtagaatgat
     2221 tctgccccct atatataaaa attattctca tatttcttga ctgatagatt ttctcctatt
     2281 attatctata aaaattctta gtaaggagtg ataaggttga agaaagaatt tcttatgatc
     2341 cctggaccaa cacctgtacc ggatagtgta ttgagaaaaa tgtctgtgcc tatggttaac
     2401 cataggggaa aagaatttgc tcagatactc aagaatgtta ctgaaaagat gaaaaaggtt
     2461 tatcttacag agggggatgt tattctcctg acatcttcag gatctggggg gatggagtct
     2521 gcagttgtaa acttcttatc ccctggtgat aaagccattt tttgtgtctc tggtacgttt
     2581 ggagatagat ggtcaaagat tgctcaggta tatggagcca atgttatcag agtagaggtt
     2641 cctcccgggg agggtgtcac gctggataag atagaagatg cattaaagaa gaatcctggg
     2701 gtaaaagcgg tgtatataac ccacaatgag acgtcaacat cgataacaaa tgacctctct
     2761 caggttggta agcttgcaca taattatgga gctattgctg ttgtagatgc ggtaagttct
     2821 cttggggcta tagagttaag gatggatgag tggcagttag atgtggtggt aacagcatct
     2881 caaaaggctc ttttaactcc tcctggtatt gcctttgcag ctgtaaataa tagagcctgg
     2941 gaattaaata aatctgcaaa aatgccaaga ttttatttcg accttggaga ggcaaagaag
     3001 atggctgcaa atggtgagac tccatttacg cctgcggtct ctcaggtctt tgctatagat
     3061 accgccctgg acattttact ctcagaagga ttggagaatt cctggaagag acatcgcctc
     3121 cttggtaaag ctaccagaga aggggtaaag gctctggggc tgaa
//
LOCUS       DAOTKU010000163         3146 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_25456_length_3146_cov_3.239081, whole genome shotgun sequence.
ACCESSION   DAOTKU010000163 DAOTKU010000000
VERSION     DAOTKU010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25456_length_3146_cov_3.239081"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            224..1081
                     /locus_tag="P5310_04780"
     CDS             224..1081
                     /locus_tag="P5310_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019004708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HRU32708.1"
                     /translation="MEDIDISFLENKAKEIRRNIVDMIYHSGAGHIGGSLSSTDILVT
                     LYYKIMRIDPRNPVWTDRDRFILSKGHSVEGYYTILADLGFIDREELKTYCKFNSRLT
                     GHPTVKIPGVEANTGSLGHGLSLGVGMALAGKMDKKDYKVYVLMGDGEQAEGSIWEAA
                     MCARHYKLDNLIGIVDRNKLQIGGPTEYVMSLEDLSAKWASFGWEVVEVDGHNISQLV
                     TVFESVPVKYNKPTLILAHTVKGKGVSFIEDKPEWHHKIPTPEEYTRMIRELNQEDDN
                     VREDIGSAV"
     gene            1050..1997
                     /locus_tag="P5310_04785"
     CDS             1050..1997
                     /locus_tag="P5310_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013411332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HRU32709.1"
                     /translation="MSEKTSVLPCRKAFSEALFELAKEDRDIVVLTSDSRGSASVENF
                     AEHLPGQFIDVGIAEQNLISIASGLAGCGKKPFIFAPACFLSTRSIEQIKIDVAYTNR
                     DVKIIGVSGGVSYGPLGSTHHSLEDIAFMRAIPNMTVILPCDVNQTKALVKSLLSYNK
                     PAYIRMGRNPVENVYDENIFFNIGKASLLSDGADATIIATGEVVKTAYDAALILRDKG
                     IYTRVIDMHTVKPIDEEIIIESAQKTEFIVTVEEHNIRGGLGSAVAEVLIKRLPVKMY
                     MAGIPDEFSITGSQKEILEYYNITPEGISNIVLKELRRG"
     gene            2037..3134
                     /locus_tag="P5310_04790"
     CDS             2037..3134
                     /locus_tag="P5310_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013782165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine corrinoid protein 2"
                     /protein_id="HRU32710.1"
                     /translation="MRYKDNWEETKSRFVAWWRHSRIDRPMMRVIGRLEDPVEPLEII
                     EPPRTPMEYHLDVDRKVKELRNFCRTHLFLAESFPSLNINIGPGSMATYLGSEPVFSW
                     DTVWYTPCVTSDWSDWGPLRYDEDNYWWKLHLSLIRKAKELAGDDFLVNIPDIIESID
                     ILSSLRGAQKFIYDLIDSPDIVKKYIKQTDELYFKYYDAMYEIVKCTDGSSSYTVFDI
                     WGPGKTAKVQCDFSAVMSPKQFHEFFIPSLTYQCNRLDYVLYHLDGPDAIKHLPAILK
                     VEKVNAIQWTPGAGKPDGGSEDWYPVYDMVRNAGKSLWISIEEGNFDDWIKKADKIVK
                     RYGADGLYLLFPITELEVAEELIRKAEMDWR"
BASE COUNT         1042 a          525 c          729 g          850 t
ORIGIN      
        1 ttaccgccgg tagggagttg gagtaaatac tcctcaccca tccccgaagg taacactatc
       61 aacattcaat tcttgagata ttatacaata atccctgtaa catctgcaag cagattctgg
      121 tcatacaaac ctttttttac cacctcttcc tcattccttt ttatgttgcc aagcttattc
      181 atataagtgt aaaatattct aaataagata taaggagaag atgatggagg atatagacat
      241 ctcattcctg gagaacaagg caaaagagat aagaaggaat atagtagata tgatttatca
      301 ttcaggggca ggacatatcg gagggtctct ttcatctacc gacatactag ttacacttta
      361 ttataagatc atgaggatag accctaggaa tcccgtctgg accgacagag atagatttat
      421 cttaagcaag ggacatagtg tggagggata ttatactatt ctggcagatc taggatttat
      481 agatagagaa gaactgaaaa cctactgtaa attcaattcg agattaacag gtcatccaac
      541 ggtcaaaatc cctggagtag aagctaatac cggctcttta gggcatggac tctctcttgg
      601 ggtagggatg gctcttgcgg ggaagatgga taaaaaagat tataaggtgt atgtattgat
      661 gggagatgga gaacaggcag agggttctat atgggaagca gctatgtgtg ccaggcatta
      721 taaactggac aatcttattg gtatagtgga caggaataaa cttcaaattg gcggacctac
      781 cgagtatgtt atgagtttag aagatctgtc tgcaaagtgg gcttcctttg gttgggaagt
      841 ggtagaggtg gacggacaca acattagtca attagtaaca gtatttgaga gtgtgccagt
      901 aaaatataat aaacccactc tcatactggc tcatactgta aagggtaagg gggtatcatt
      961 cattgaggac aaaccagaat ggcatcacaa gattcctacc ccagaagagt ataccagaat
     1021 gatcagggaa ttaaatcagg aggatgataa tgtcagagaa gacatcggtt ctgccgtgta
     1081 gaaaggcttt tagcgaggca ttatttgaat tggctaaaga agacagggat atcgttgttt
     1141 taacttctga ctccagaggg tctgccagcg tagaaaattt tgcagaacat cttccaggac
     1201 agtttataga cgttgggata gcagaacaga acctgataag tatagcaagt ggacttgcag
     1261 gttgtgggaa gaagccattc atttttgctc cagcctgttt tttaagtaca aggagtattg
     1321 agcagataaa gatagatgtt gcctatacaa acagggatgt taagataatt ggagtaagcg
     1381 gaggtgtcag ttatggtccc ttaggttcta cccatcactc cttagaagat atagccttta
     1441 tgcgagccat tcccaatatg actgtaatac tcccctgtga tgtaaatcag acaaaagccc
     1501 tggttaaatc tcttctatct tacaataagc ctgcttatat cagaatggga aggaatcctg
     1561 ttgaaaatgt ctatgatgag aatatctttt tcaacatagg caaggctagc ctgttatcgg
     1621 atggagctga tgccactata attgccacag gagaagttgt caaaacagcc tatgatgctg
     1681 ccttaattct gagggataaa ggtatatata ctagagttat agatatgcat acagttaagc
     1741 cgatagatga agaaattata atagaatctg cccaaaaaac tgaattcata gttaccgttg
     1801 aagaacacaa tataagggga gggctggggt ctgctgtggc agaagtatta atcaagagac
     1861 ttccggtaaa aatgtatatg gcaggcatac ctgatgagtt ttcgataact ggctctcaaa
     1921 aggaaatact ggagtactat aacatcactc cagaaggtat cagtaatatc gtattgaaag
     1981 agttgaggag gggataaaaa tcactgcagg ggcaataaag ggctaggagg aaatttatga
     2041 gatataaaga taattgggaa gaaactaaaa gtagatttgt cgcctggtgg aggcactcca
     2101 ggatagatag acctatgatg agggttatag gaagactgga agaccctgta gagcccttag
     2161 aaataataga gcctcccaga accccaatgg aatatcatct agatgtagat agaaaagtaa
     2221 aagaattacg taatttttgc cgaactcacc tcttcctggc agaatcattt ccctctctaa
     2281 atataaatat aggaccaggc tcaatggcta cgtatttagg ctcagaacct gtattctcct
     2341 gggatacggt atggtataca ccatgtgtta cttctgactg gtctgattgg ggacctctga
     2401 gatatgatga agataactac tggtggaaac tccacctgag cctgatccgt aaagctaagg
     2461 agttggcagg cgatgatttc ctcgtaaata taccggatat aatagagagc atagacattc
     2521 tttcctcttt aaggggggca caaaaattta tatatgacct tattgatagc cctgatatag
     2581 taaaaaaata tataaagcag acagatgagc tctactttaa atactatgat gccatgtatg
     2641 aaatcgtaaa atgtacagat ggaagcagta gctatacagt attcgatata tggggacctg
     2701 gtaagaccgc taaagtccag tgtgattttt cagctgttat gtctccgaaa cagtttcatg
     2761 agttcttcat accgtcactt acatatcagt gtaatagact ggattatgtt ttatatcacc
     2821 tcgatggacc agatgctata aaacatctgc ctgccatact taaagtagaa aaagtgaatg
     2881 ctatccagtg gactccaggg gctggtaaac cagatggggg tagtgaagac tggtatcccg
     2941 tatatgatat ggtaagaaac gctggaaaat ctctctggat cagtattgaa gagggaaatt
     3001 ttgatgattg gataaaaaaa gcagataaaa tagtaaaacg ttatggagca gacggactgt
     3061 atctcctatt tccaataaca gaattagagg tcgcagaaga attgataaga aaagcagaga
     3121 tggattggag ataaggaaaa attaga
//
LOCUS       DAOTKU010000164         3138 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_25534_length_3138_cov_4.341550, whole genome shotgun sequence.
ACCESSION   DAOTKU010000164 DAOTKU010000000
VERSION     DAOTKU010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3138
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25534_length_3138_cov_4.341550"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /locus_tag="P5310_04795"
     CDS             complement(<1..730)
                     /locus_tag="P5310_04795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HRU32711.1"
                     /translation="MRKINTLDIAGIIEDLCKRVNYKLDETVISTIEEASRRESSPIG
                     KRVFSQMLTSYQLAEEEKTPLCQDTGMVSVFLEIGQEVFLKGIPLEEAINTGVRRAYT
                     GSFFRNSIVDDPIGTRRNTLDNTPCIINTRLISGDKINVKLLIKGFGSENATALKMFS
                     PTSSKNDIEEFILDTVRERGPNACPPLFIGVGIGGTADYAVKLSKEALLNLEPMDEWE
                     LSILDKINSLRIGPAGLGGDVTALY"
     gene            complement(738..2213)
                     /locus_tag="P5310_04800"
     CDS             complement(738..2213)
                     /locus_tag="P5310_04800"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HRU32712.1"
                     /translation="MRDLSERFIVLEGSDVDKIRAYDKLLTKFVILRRLKTTLGLGKL
                     IPLLEKITNLKDNFLKVLGYQILENGTVYSIEEIPPQGEVEPASNIKELLNITRYVGR
                     SLESINELGIRVKLPPKEELYKDEGGNYFVSPLSLIDLNNPDKPQEKGEVLNLLASFF
                     LLYAPQNLKTTVSSLIERNIICKLDNADAFVNMLNTLLIQEERMKIKTEDKKNVSEAK
                     KEKRRVNPWVIIVIVLLLVAGISVGVLYSVGKKLFFSEETVPDVIVPDIVNKSLPEAF
                     NVLKANNLNIEISGLDYSSVIPAGNVISQDPISGTKVKSGRSINVIISKGIEFVNVPN
                     IIGQELGNARKMLESVGLKIGDIKETYSRTSPDGIVISQNPKYGSITPKNSLVTVEIA
                     RHLPNTMPNLIGKTQEEAEGLIDSLGSYKLIIKEAESDTPGVVISQNPLPNTSIIQEV
                     PRIEITIGIPKRVVPQVVPSSPAETGTQEETLPAQDTLPQE"
     gene            complement(2197..>3138)
                     /gene="rlmN"
                     /locus_tag="P5310_04805"
     CDS             complement(2197..>3138)
                     /gene="rlmN"
                     /locus_tag="P5310_04805"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0002935 - tRNA
                     (adenine-C2-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0070040 - rRNA
                     (adenine-C2-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HRU32713.1"
                     /translation="KQIIEWIYKKGKLDFDGMSNLPAELREFLSCNFSILPFEIIQSY
                     KSEDTVKFLFRTDDDLIFESVVIFHPDRGILCLSTQIGCPVRCRFCASGENFYRNLRY
                     EEIIGQYIFARNYIKRDIRGIVFMGMGEPLLNEESLYRTIDVLTEIIGISPRHITVST
                     SGIPKGILNIARRNPKVRLALSLWTPEEEEREKLVPVSKIYPLSEVIESLRRYVEITK
                     NRVSIEYTLLKGINDGIGDVHKLISLFRDMPVFFNVILYNPKDEDTKIYQTSVEEALR
                     FVDLLKRSGKEAHLRVSKGGKISGACGQLRAVYERSK"
BASE COUNT          860 a          724 c          502 g         1052 t
ORIGIN      
        1 catacagggc ggtaacatct cctccaagcc cagctggacc tatcctcaag ctgttaatct
       61 tatcaaggat agaaagttcc cattcatcca taggttcaag attcagaagg gcctctttcg
      121 aaagctttac ggcataatct gccgttcctc ctattcctac cccgataaag agtggtggac
      181 aggcatttgg acccctttct ctcacagtat ccagtataaa ttcttctatg tcgttcttac
      241 tcgaagtggg agaaaacatc tttaatgcag ttgcattttc acttccaaat ccttttatta
      301 ataatttcac attgatttta tctccggaga tcagcctggt attgatgatg cagggtgtat
      361 tatctagtgt attccttcta gttccgatag ggtcatctac tatagagttt ctaaaaaagg
      421 aacctgtata ggctcttcta acacctgtat ttatagcctc ttctaaaggg attcccttta
      481 aaaatacctc ctgccctatt tctaaaaaga ccgataccat accggtatcc tgacagagag
      541 gcgttttttc ttcctctgcc agctgataac tggttaacat ttgagaaaaa acccttttcc
      601 ctatgggaga actctctctc ctggatgctt cctctatcgt agagattacc gtttcatcca
      661 gtttataatt aactctctta catagatctt ctataattcc agcgatatct aatgtgttta
      721 tctttctcat agatacctca ctcctggggt aaggtatctt gagctggtag agtctcctcc
      781 tgagtccccg tctcggcagg tgatgagggg acaacttgag gtactactct tttagggata
      841 ccaatggtga tctctatcct ggggacctcc tgtattatgg atgtatttgg aagaggattc
      901 tgagagatga caaccccagg ggtatcactt tctgcttctt taattattaa tttataagac
      961 ccaagagaat ctatcaatcc ttcagcctct tcctgcgttt taccaatcag gttaggcata
     1021 gtgtttggta gatgtctggc tatctctaca gtcaccaggg aatttttggg ggtaatgcta
     1081 ccatattttg gattctgaga aattactatc ccatcagggg aagttctact ataagtttct
     1141 ttaatatctc caatctttag ccctaccgat tccagcatct ttctagcatt tcccagttct
     1201 tgcccgataa tattggggac attaacaaat tctatcccct tgcttataat aacatttata
     1261 cttcttccag actttacttt tgtcccactt atcgggtcct gtgatattac attaccagca
     1321 ggtataacac tgctataatc taaccctgaa atttcgatat taagattgtt agcctttaaa
     1381 acattaaaag cttctggtaa actcttattc actatatctg gaactattac atctgggacc
     1441 gtctcttcag agaagaataa tttcttaccc accgagtata gtactcccac acttatccct
     1501 gctaccagga gaagtactat tacaattata acccaggggt ttacccttct cttttccttt
     1561 ttagcctcag acacattctt tttatcctct gtctttatct tcatccgttc ctcctgaatc
     1621 agtagtgtat tcagcatatt aacaaaggca tcggcgttat ccaacttgca tataatattt
     1681 ctctctataa gagaagatac agtggtcttt aaattctggg gagcgtataa cagaaagaaa
     1741 gatgccagca ggttaagaac ctctcctttt tcctgcggtt tatcaggatt gttcaggtct
     1801 attaagctga gtggggatac gaagtagttt cctccttcat ccttatatag ctcttctttc
     1861 ggtggtagtt ttacccttat accaagttca tttatactct ccagagatct acccacatac
     1921 ctggtaatat ttaacaattc ttttatatta ctagcaggct caacctctcc ctggggagga
     1981 atttcctcta tggaatatac agttccattt tccagaatct gataacccag gactttcaaa
     2041 aaattgtcct tcagattggt tattttctcc aggagaggga taagtttccc taaccctaat
     2101 gttgttttta atctccttaa tattacaaac tttgtcagta acttatcata agcccttatc
     2161 ttatcaacat cagaaccttc aagcacaata aatctttcac ttagatctct catataccgc
     2221 cctcaactga ccacatgccc ctgatatttt acccccctta ctaaccctga ggtgagcttc
     2281 tttacctgac ctcttcaaca gatctacaaa tctcaaagcc tcttccactg aagtctggta
     2341 gatttttgta tcctcatctt ttggattata caatataaca ttgaaaaata ccggcatatc
     2401 tctaaaaaga gagattaatt tgtggacatc gcctattcca tcattgatcc ctttaagaag
     2461 cgtatattca attgataccc tgttttttgt aatctccacg tatctcctta aactctctat
     2521 aacctcactt aagggataga tcttagatac aggcactaac ttttccctct cttcttcttc
     2581 tggagtccag agagaaagag ccaatctgac ctttggattt cggcgggcta tgtttaaaat
     2641 ccctttaggt atacctgaag tagagacggt tatatgcctt ggagagattc ctattatttc
     2701 tgtaagtaca tctattgttc tatagagtga ctcttcatta agtaatggct cccccatccc
     2761 cataaagact atccctctta tatctctctt tatatagttc ctggcaaata tatactgtcc
     2821 tattatctcc tcatatctta ggtttctata aaaattctct ccacttgcac agaatctgca
     2881 cctgacagga caccctatct gggtagagag acagaggata cctctatcag gatgaaatat
     2941 aactaccgat tcaaagatca ggtcatcgtc agtcctgaac aaaaatttta cggtatcctc
     3001 agacttataa ctctgaatga tctcaaatgg taatatagag aaattacagg acaaaaactc
     3061 acgcaattca gcagggaggt tgctcatacc atcaaaatct aactttccct ttttatatat
     3121 ccattcaata atctgttt
//
LOCUS       DAOTKU010000165         3136 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_25555_length_3136_cov_2.981500, whole genome shotgun sequence.
ACCESSION   DAOTKU010000165 DAOTKU010000000
VERSION     DAOTKU010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3136
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25555_length_3136_cov_2.981500"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /locus_tag="P5310_04810"
     CDS             complement(<1..731)
                     /locus_tag="P5310_04810"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRU32714.1"
                     /translation="MSYRILYILPYMNLGGTEVHTVELIKGIRDEYDVLVAGPDGPGI
                     SLLEENDISYTSLPGLTPYNIRVYKDILSRIIRDYKPDLIHIQGRYEPVLFSKKISPS
                     TPVILTCHGYGNAVSPFDYMFTAITGNRWGDRVITVCNYDKELLLKFGLNPEKVSLIY
                     NGISSIDERKPLPYSISLKIGTIASLIKRKGISYLIEAMSLITKRFKDVGLFIIGEGK
                     ERISLESLVNQLNIKNHVYFLGGLP"
     gene            complement(755..1822)
                     /locus_tag="P5310_04815"
     CDS             complement(755..1822)
                     /locus_tag="P5310_04815"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRU32715.1"
                     /translation="MRSPKVLYLLPYFELGGTERHLLELARMAIGDFSILVVSPGGDG
                     VSLLEEYGIPHIKVPTLTLFNILKYRSIIKKLILEFRPYIIHIHGAHELVFIVKNLFP
                     DIPVIFTSHGYASNFPLFDYTLSADICDRYSDKVIAVSEYERGLLLKRGLKDSKVCTI
                     YNGISPVEGNLNLSLPRDVSFKIVTVARLTRKKGIKYLIEAFGLISKKYKDTGLFIIG
                     EGEERKYLEKLVGRLNLRNVFFLGKIPDAGKYLNNFDIFVLPSLSESLGISIIEAYAE
                     KRAVIATKIGGIPELIVDGETGLLVAPGDSYALADSIEKLILDRALRDRLAYNGYNRF
                     LGYFTSEKMYEKTLNVYKSFL"
     gene            complement(1809..2945)
                     /locus_tag="P5310_04820"
     CDS             complement(1809..2945)
                     /locus_tag="P5310_04820"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRU32716.1"
                     /translation="MGKDINDKRIKLEETALALFTFFMGMGGGLLSPFIYIFYRLFLI
                     KNWHLKFDLYSILLLIGFLMSVIFSEYRIFSLQNFLVFFFMYIVYSLLRTSNIARESS
                     DRIFDYWLFGSTLLAFGGMVGYLYSGVYADTPFMGKNGIGTLLATAVPISQVKLCCRG
                     KLYDYLSFILITATLLLSMSQGAWIGLFLAEILLFVFGDKKIRKSILILALLLIVLFF
                     IFSVHSFATGNRFLDFFYTRLDMTSNSKIDRIYIWRSSIKMFLDHPITGVGLGTFSLE
                     YPDYILPGAREIIVSFAHNLPLNLLVETGIVGFLTFFLFLIYLYRRCLTAFKISRDNF
                     YLMILASLTAYMGHQLFDGTMWSLHIGIVFWLLGALILNFDEKS"
BASE COUNT         1042 a          658 c          529 g          907 t
ORIGIN      
        1 ttgggtaacc ctccaagaaa atagacatga ttttttatat tgagttgatt tacaaggctt
       61 tcgagactta tcctttcctt tccctcccct attataaata aacccacatc cttaaatctt
      121 tttgtaatca gactcatagc ctctatgaga taactgatac cttttctttt aataagacta
      181 gcaatcgtac ctatcttcag gcttatactg taaggcagag gctttctctc atctatagaa
      241 gaaatcccat tgtatatcaa ggataccttc tcgggattta agccaaattt taataaaagt
      301 tctttatcat agttgcatac cgtaattact ctatctcccc atctatttcc tgttatcgca
      361 gtaaacatat agtcaaaggg ggaaactgca tttccatatc catgacaggt cagaataacc
      421 ggagtggagg gagaaatctt cttactgaag agtacaggtt catacctccc ctgtatatgg
      481 attaaatctg gtttataatc ccttattatc ctcgataaga tatccttata gacccttatg
      541 ttatacggag taagtccagg aaggctggta taagatatat cattctcttc caggagagaa
      601 ataccaggac catccggtcc tgccactaat acatcatatt catctctaat accttttatt
      661 aactcaacgg tgtgaacctc tgtccccccc agattcatat atggaaggat ataaagtatc
      721 ctgtaagaca tagaccaatt tgtttatcct cctttcataa gaaactttta tatacattta
      781 atgtcttctc atacatcttc tctgaagtaa aatatcctaa aaatctatta taaccattat
      841 aggcaagtct atctctgaga gccctgtcca atataagttt ctctatagaa tcagccaggg
      901 cataactgtc tcccggcgca accaaaagac cagtctcacc gtctacaatt aactccggaa
      961 taccaccaat ttttgttgct ataaccgccc tcttttccgc atacgcttcg ataattgata
     1021 tacctaacga ttcagagagg gatggcagga caaagatatc aaagttattt agatatttac
     1081 cagcatcagg tatcttcccc agaaaaaaga catttctcag attcaacctg ccgactaatt
     1141 tctccaggta tttcctctct tctccctccc ctattataaa taatcctgta tccttatatt
     1201 ttttagatat aagaccaaag gcttctataa gatattttat ccccttcttt ctggtcaatc
     1261 ttgctacagt aactatttta aaagatacat ctctgggaag ggacaggttc agattaccct
     1321 ccacaggtga gataccattg tatatggtac agaccttact atcttttaat cctctcttta
     1381 aaagaagtcc tctttcatac tcagagacag caatcacctt atctgaatat ctatcacata
     1441 tatcagctga cagggtgtaa tcaaataatg ggaagttcga tgcatatccg tgtgaggtaa
     1501 atattacagg gatatcgggg aagagatttt ttactataaa taccagctca tgagctccat
     1561 gtatatggat tatataaggt ctgaattcca gtatcagttt ttttataata ctcctgtatt
     1621 ttaagatatt aaaaagagta agtgttggaa cctttatatg aggaattcca tactcctcaa
     1681 gcagagaaac cccatcacca ccgggagaaa ctaccaggat agagaaatcg ccaattgcca
     1741 ttctcgccaa ctccaacaga tgtctctctg ttcctcccaa ttcaaagtag ggaagaagat
     1801 aaaggacctt aggacttctc atcgaagttt aatataagag cccccaataa ccagaagact
     1861 atcccaatat ggagggacca catagttcca tcaaacagct gatgccccat ataggctgta
     1921 agagaagcga gaatcatcaa ataaaagttg tctctgctga tcttaaaagc agttagacat
     1981 ctcctgtaga gatagatcaa aaataaaaaa aaggtcagaa aacccacgat accggtctcc
     2041 acaaggagat ttaatgggag attatgggcg aaagaaacta taatttcgcg agctccagga
     2101 agtatatagt ctggatattc taaagagaac gttccaagac ctaccccggt tatgggatgg
     2161 tcaagaaaca tctttatact ggacctccag atatatatcc tgtcaatctt ggaattagag
     2221 gtcatatcca gccttgtata gaagaaatca aggaatctat ttccagtggc aaaagaatgc
     2281 actgaaaaga taaaaaacag cactattaga aggagagcaa gaataagtat gctcttcctt
     2341 atttttttat ctccaaagac aaacaaaagt atttccgcta agaataaccc gatccatgcc
     2401 ccctgagaca tacttaatag aagggtggca gttataagga taaacgatag ataatcgtaa
     2461 agtttccctc tacagcataa ctttacctga gatattggca cagcggtagc cagtaaagtt
     2521 cctattccat tcttacccat gaatggtgtg tctgcataga caccgctata gaggtatcca
     2581 accattcctc caaaggcaag tagagttgaa ccaaacaacc agtagtcaaa gatcctatct
     2641 gagctttctc tggctatatt actggttctc agaagagaat atacaatata cataaagaag
     2701 aagaccagaa aattctgcag tgaaaatatc ctgtattcag aaaagataac agacatcaga
     2761 aaccctataa gaagcaggat agaatacaga tcaaacttta agtgccagtt ctttatcaaa
     2821 aacaatctgt agaagatata tataaaggga ctgagtaaac caccccccat acccataaaa
     2881 aatgtaaaca gggcaagggc agtctcttcc agttttatcc tcttatcatt gatgtctttt
     2941 cccataccat aattatttta acccaatact caagtatcgt caaattagga actctgatag
     3001 aaaaggtggt agaataataa gaaacattta taaggaggct gagaagatgc gatatcttcg
     3061 tcctgttgaa ccatttctag ctctctacga tgaggggaga gtctatgaat ttaaggctca
     3121 gaccgtagag gagtgg
//
LOCUS       DAOTKU010000166         3105 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_25847_length_3105_cov_3.479344, whole genome shotgun sequence.
ACCESSION   DAOTKU010000166 DAOTKU010000000
VERSION     DAOTKU010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25847_length_3105_cov_3.479344"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..783)
                     /gene="mazG"
                     /locus_tag="P5310_04825"
     CDS             complement(22..783)
                     /gene="mazG"
                     /locus_tag="P5310_04825"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HRU32717.1"
                     /translation="MKTLGEKLEELLRIGKVLREKCPWDAEQDFDSIKYTLREEAYEV
                     IDTIEEKDFLHMKEELGDLLFNIFFMSIIAEETRLFTLEDVVDKIIDKLIKRHPHVFG
                     DIRVNSSEEALSNWEKIKREENRGVMDVPMSLPSLALAHKVQEKARRIGFDWDDYIGP
                     REKINEELGELDKAIEEKEFLHIEEEIGDLLFSVVNTARLLGIDSESALRSTVKKFIK
                     RFSRMEELAKERDISLEGLTLEEMDKLWEEIKGGE"
     gene            complement(786..>3105)
                     /gene="mfd"
                     /locus_tag="P5310_04830"
     CDS             complement(786..>3105)
                     /gene="mfd"
                     /locus_tag="P5310_04830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HRU32718.1"
                     /translation="FIIFSSHFDTSMRHLKVEIGDDILGDTPPLFSGDIEKIKKNLES
                     YLNSGYEIYLYTVQAKRLQSLMRGKKGINFYKKKLSGGVIIPSSKIMILSDRELFSYE
                     FVPAFASPVEGRVEIPFNIGDYIVHPYHGIGRFSGIETQLVEKVPREFIVLEFAENDR
                     LLVPVEQAGRLDKYRSFDDTPPPIYRLKSPEWARTKAKVEKDVKNIAKDLIEVNAKRE
                     VMPGIPFKPYPEMEKLLEESFPYDETSDQREIIERVKKSMEKSRPMDFLVCGDPGYGK
                     TEVAIRAAFRAVLNGKQVALLAPTTILAHQHYETFRERFATLPVTIAMLSRFTPTRDV
                     RRIKEGIANGSIDIIIGTHKILGKDIRFKDLGLLIIDEEHKFGVEQKEKLKGLFPLID
                     VLTLSATPIPRTLSMSLYGLKEIGIIETPPEGRIPIETYVGEYDEKVVRLAIERELSR
                     NGQIFYIHPRITGVRELESRLKDMFPELSIDILYGTMDEEEVEERFWRFYKGETKLLV
                     STAIVESGIDIPNANTLIIEEGEKFGLAQLYQLRGRIGRAGRKSYAYIFHSKSLTPDG
                     EERLRAIYENSSFGSGFNIAMRDMEIRGLGNLLGKEQHGHIIAVGFDMYLKMLEDAVR
                     ELRGEPVKWRLEPTIIEIGIPALIPDTYIPSPYKLEIYRRLSLASTLEEVEDIGSELV
                     DRFGKMPEEVENLLNVTIVKIFAEKRGIKKLSITDDRVTVVFRDANGDGSILRILGKF
                     GQTKKINDENWIIRWWEDRNRKLIKIKEAIKP"
BASE COUNT          785 a          784 c          476 g         1060 t
ORIGIN      
        1 ctactgctat atcaagcata tctattcgcc accttttatc tcttcccaga gcttatccat
       61 ctcttccagt gttaatcctt ctaaggatat atctctctcc ttagccaatt cttccatccg
      121 gctaaatctc tttataaatt ttttcaccgt acttctgagg gcagactctg agtcaatacc
      181 cagaagcctc gccgtgttta caactgagaa tagcaggtcc cctatctctt cttctatatg
      241 taagaactct ttctcctcta tagccttatc cagctctccc agctcttcgt ttatcttttc
      301 tctgggacca atataatcat cccagtcaaa tcctatcctc cttgcctttt cctggacctt
      361 atgagccagt gcaagagagg gcaggctcat ggggacatcc ataactcctc tgttttcctc
      421 tctctttatc ttctcccagt tagacagtgc ctcttctgag ctgttaactc ttatatctcc
      481 aaatacgtgg ggatgccttt ttatcagttt atctataatc ttatccacca catcttctag
      541 ggtaaataat ctcgtctctt cagctataat tgacataaag aatatgttaa agagcaggtc
      601 tcccagctct tctttcatat gtaagaagtc tttctcctct atagtatcta taacctcata
      661 ggcttcttct ctcaatgtat atttgattga atcaaagtcc tgttctgcat cccaggggca
      721 tttctctctc aataccttac ctatcctgag cagttcctct aatttttccc ctaaagtctt
      781 catattcaag gcttaattgc ctcctttatc tttatcaatt ttctattcct gtcttcccac
      841 catcttatta tccaattttc atcgtttatt tttttagttt gtccgaactt tccaagaatc
      901 ctcaatatgc ttccatcacc atttgcgtct ctaaatacca ccgttaccct gtcgtcagta
      961 attgacaatt tctttatccc tcttttttcg gcaaagatct taactatcgt tacatttaac
     1021 aggttctcca cctcttctgg cattttaccg aatctatcta caagctctga tccgatatcc
     1081 tctacttctt ccagagttga agcaagagat aatctcctgt atatttccag tttataaggt
     1141 gagggaatat aggtatctgg tatcaatgca gggataccga tctctataat cgttggctct
     1201 aacctccact tcactggctc ccctctcaac tctctcactg catcctctaa catcttaaga
     1261 tacatatcga aacctaccgc tattatatga ccatgctgtt ctttacccag cagattaccc
     1321 agacctctta tctccatatc tctcattgct atattaaaac cagagccaaa agagctattc
     1381 tcgtatatgg ctctcaacct ttcttcacca tcaggggtga gacttttact gtgaaagata
     1441 taggcatagg actttcttcc agcccttcct atccttcctc tgagctgata aagctgggca
     1501 agcccaaact tctccccctc ctcaattata agcgtattgg cattggggat atctatacca
     1561 ctctcaacta tagcagtgga tacaagtaat tttgtttctc ccttataaaa tctccagaat
     1621 ctctcttcaa cctcttcttc atccatagtc ccatagagta tgtctatgct cagttcaggg
     1681 aacatatctt tcaatctgga ttcaagctct ctgacgccgg tgatgcgggg gtggatataa
     1741 aatatctgcc cattcctgga tagttccctc tctattgcaa gtctcacaac cttctcatcg
     1801 tactctccca catacgtctc tatggggata cgtccttcag gtggagtttc tattatacct
     1861 atctctttca gcccatagag ggacatagac agtgtcctgg gaataggtgt tgccgaaaga
     1921 gttaatacgt ctataagagg aaatagccct tttaatttct ccttctgttc gactccaaat
     1981 ttatgttctt catctattat aagaagacca aggtctttaa atcttatatc tttaccaagt
     2041 atcttatggg tccctataat tatatctata ctgccatttg ctataccttc ctttatcctg
     2101 cggacatccc ttgtgggggt aaatcttgag agcatagcga tagttaccgg aagtgttgcg
     2161 aatctctccc taaatgtctc ataatgctga tgagccagta tagttgttgg tgcaagaagt
     2221 gctacctgct taccattcag aacagctctg aatgctgctc ttatagcaac ttcagtctta
     2281 ccatacccgg ggtctccaca gacaagaaaa tccatcggtc tggatttttc catacttttc
     2341 ttaactctct ctattatctc tctctggtct gatgtctcat cgtatgggaa actctcctct
     2401 aaaagttttt ccatctcggg atagggttta aatggtatac caggcataac ctccctcttt
     2461 gcatttacct ctatcaggtc ctttgcgata ttttttacat ccttttctac cttagccttt
     2521 gttcttgccc attcagggct ctttaatctg tatatgggtg gaggggtatc atcaaaactc
     2581 ctgtatttgt caagtcgtcc tgcctgttcc acagggacaa gaagtctatc attttcagca
     2641 aactcaagta ctataaattc cctggggact ttttctacaa gctgggtttc tatcccggaa
     2701 aatctaccta tcccatggta aggatgtact atataatctc ctatgttaaa cggtatctca
     2761 accctgcctt ccaccggaga ggcaaaggca ggtacaaatt catagctaaa taactcccta
     2821 tctgagagaa tcataatctt tgaagagggg ataataacac cacctgatag tttcttctta
     2881 tagaaattta tccccttttt ccctctcata agagattgca atctcttagc ctgaacagtg
     2941 tagagatata tctcataacc actattcagg taggactcta aattcttctt tatcttttca
     3001 atgtcaccag aaaagagtgg aggagtatct cccaggatat catctcctat ctccaccttt
     3061 aaatgtctca tagatgtatc aaaatgagag gagaatatta taaat
//
LOCUS       DAOTKU010000167         3102 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_25872_length_3102_cov_3.258615, whole genome shotgun sequence.
ACCESSION   DAOTKU010000167 DAOTKU010000000
VERSION     DAOTKU010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3102
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25872_length_3102_cov_3.258615"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1582)
                     /locus_tag="P5310_04835"
     CDS             complement(<1..1582)
                     /locus_tag="P5310_04835"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HRU32719.1"
                     /translation="MSSTYYSSRMRFSRDYRRNLVDMHIEDWDEKFLSQFDPKRYVEL
                     LKVANVTTAMIYANSHVGYCYWPTKIGHMHGGIKGRDILGEVIELCHKEGINVVIYYS
                     LIYNNWAYEKEPSWRIVLADGKTSREVQDRSGRYGVCCPNSGYREFALSQIDELCSNY
                     NFEGIFFDMTFWPGVCYCKSCQERFFKETGEELPKVINWEDPGWVRFQRKREEWLSEF
                     AHLATNKVKSINPNISVEHQFSTCVAAWSLGVTSEISEASDYCGGDFYGGLLQESFIC
                     KLYYNLTDNQPFEFMTSRCINLRDHTTTKSKELLEAQVYSALSNGGAFLFIDAIDPIG
                     TLNKKVYETMGNIYNTVRTYEEYIDGKLCQDIGIYFSFESKFDPKDNGRDVLESTHKQ
                     PHLDSALGVAKSLISNHIPFGIITKKNLNELSRYKVIILPNILMMDEEEVTAFREYVA
                     SGGKLYASYYTSLFDKNGNKREDFMLADVFGVSYTGETQEDVTYIAPCREVLESFGDV
                     SSQYPFTLFSSQIKTNPLK"
     gene            complement(1620..2732)
                     /locus_tag="P5310_04840"
     CDS             complement(1620..2732)
                     /locus_tag="P5310_04840"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRU32720.1"
                     /translation="MDNSWNLRKVFREVIAFHEFEYFPHFEAWGFWNETIDRWHKEGL
                     PLDKTPWEFFKLDQFMSNAPEELSYYTPNRITQIPYWPPFEYKLIEETDTYIVQRGSD
                     GIVKKQLKDKTSMPQFLDFPVKSRKEWEDLKWRLDPHIKDRYKGVKEISFSFNNRNTL
                     VPFVICGGYGFPRNLFGEVTLAYTYYDDPNLIHEIMRHWVYFYTGIADDLCPLIDFDY
                     VYLWEDMAYKTSSLISPKHFCEFIFPYYKELITYLREKYKLDIFLVDSDGNNFSLLPL
                     FIESGVNIFVPCEINAGMEPLLIREKFPDLALLGGINKQVLSRGKEEIKREVLRKVPK
                     MLESRGYFPSVDHLIPADVSFENFCYYIEVLREIEK"
     gene            complement(2803..>3102)
                     /locus_tag="P5310_04845"
     CDS             complement(2803..>3102)
                     /locus_tag="P5310_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32721.1"
                     /translation="HGLKNYNYNEEALYIAKKFYEVSINNPSLYEWYNAETGEGQGGH
                     PFVAGISILGLFLPLELELEVKTMEIVPIQEELDISPIREKLRLETLPNLAKPEE"
BASE COUNT          988 a          638 c          455 g         1021 t
ORIGIN      
        1 ttttaagagg gtttgtcttt atttgtgagc taaataaagt aaagggatat tgagaagaga
       61 cgtctccaaa gctctctaaa acttcccggc aaggagctat ataggttaca tcttcctggg
      121 tctctcctgt gtatgagact ccaaagacgt cggcaagcat aaaatcttct ctcttattac
      181 cattcttatc gaaaagtgaa gtataataac ttgcatataa tttaccacca gaagcaacat
      241 actctctaaa ggcagttact tcttcttcat ccatcattaa tatgtttggc aggataataa
      301 ccttatatct cgatagttca ttgagatttt tcttggttat tatgccaaaa gggatatggt
      361 tacttattaa gcttttagcc acacctaaag ctgagtctag atgaggttgt ttatgggtac
      421 tttctaaaac atctctccca ttgtctttag ggtcaaattt agattcaaaa ctaaaataga
      481 taccaatatc ctggcaaagt tttccatcta tgtactcttc ataagttctc actgtattat
      541 atatattacc catcgtttca tataccttct tgtttaaggt ccctatcgga tcaatagcat
      601 ctataaaaag aaatgctcct ccattggata aagccgaata gacctgagct tctaaaagtt
      661 ctttggattt tgtagtggtg tggtctctta gatttataca tctacttgtc ataaactcaa
      721 aaggctgatt atcagtaaga ttatagtata atttacagat aaatgattct tgcaagagtc
      781 ctccataaaa atctcctcca cagtagtcac tggcttcaga tatctctgaa gtcactccaa
      841 gactccaggc agcgacacag gtagagaatt gatgctctac tgaaatattt ggatttatag
      901 actttacctt attagtagca agatgagcaa attctgaaag ccattcttct ctcttgcgct
      961 gaaatctaac ccaacctgga tcttcccagt taataacttt tggaagctct tctcctgtct
     1021 ccttgaagaa tctttcttga caagatttac agtagcatac cccaggccaa aatgtcatat
     1081 caaagaaaat accttcaaaa ttgtaattac tacaaagttc atctatctga gataatgcaa
     1141 attctctata tccagagtta ggacaacaaa ccccatagcg accagaacgg tcttgaacct
     1201 ccctggaagt ttttccatca gcaagtacta ttctccagga aggttccttt tcatacgccc
     1261 agttattata tataaggcta taataaatga cgacattaat cccctcttta tggcacagtt
     1321 cgattacctc acctaatatg tctcttccct ttataccccc atgcatatgc cctatttttg
     1381 taggccaata acaataacct acatgagaat tggcatatat catagcagtg gtaacattag
     1441 ctactttcaa cagttctaca tatctcttag ggtcaaactg agataggaat ttttcatccc
     1501 aatcttcaat atgcatatct accagattac gtcggtaatc cctggaaaat cgcattctac
     1561 tagaataata agtagaactc aatagccacc tcctatcgaa taaattctcc atcttacttt
     1621 tatttctcga tttctcttaa aacttctata taataacaga aattttcaaa agatacatct
     1681 gccggaatca aatggtctac agaagggaaa tatcctctac tttctagcat cttcggtacc
     1741 ttccttagca cttctctttt tatttcttct ttccctctgc ttaacacctg tttattaatc
     1801 cctcctagaa gagctaagtc aggaaatttt tctctgataa gaagaggttc catacctgca
     1861 ttaatttcac aaggaacaaa tatattaacg ccagattcta tgaagagagg aagtaacgaa
     1921 aaattgttac catcagaatc taccaaaaat atatctagct tatatttctc tcttaaataa
     1981 gtaataagtt ccttataata gggaaagata aattcacaaa aatgtttcgg agagattaga
     2041 gatgaagtct tataagccat gtcttcccat agatatacat agtcgaaatc aattaatgga
     2101 cagagatcat cagcaatccc ggtatagaaa taaacccaat gcctcataat ctcatgtata
     2161 agattagggt catcataata agtatacgca agagttactt ccccaaatag attccttgga
     2221 aaaccatatc ctccgcatat tacaaaaggg acaagagtat tacggttatt aaagctaaac
     2281 gatatttctt ttactccttt gtatctatcc tttatatgtg gatcaagtct ccattttaaa
     2341 tcttcccact ctttcctact ttttacagga aaatctaaaa attgaggcat agaagtttta
     2401 tcttttaatt gtttctttac tatcccatca cttcctcttt gaacaatata agtatcggtc
     2461 tcttctatta gtttatattc aaagggtggc caataaggaa tttgagtaat tcgattagga
     2521 gtatagtagg aaagctcttc tggagcatta ctcataaact ggtcaagctt aaaaaactcc
     2581 caaggagtct tatccaaagg aagcccctct ttatgccatc tgtctatcgt ctcattccaa
     2641 aaaccccatg cttcaaagtg tgggaaatat tcgaattcat ggaaagctat tacttctcta
     2701 aatacctttc ttaaattcca actgttatcc atacacttaa ttgtaacctc ctgtcaaatt
     2761 catgagcctt cttacagaaa atcgtagaat agaaactata tgttattctt caggtttagc
     2821 taaattaggc aaagtctcaa gtcttaattt ttctcttatc ggtgaaatat ccaattcctc
     2881 ttgaattgga actatctcca tcgtcttaac ttctaactct aattctagag ggagaaagag
     2941 tcccaagata gatatgcccg caacaaaagg atgtccccct tgtccctctc cagtttcagc
     3001 attgtaccat tcatagagag aaggattatt gatacttact tcataaaatt ttttagctat
     3061 atataaagct tcttcattat agttataatt cttaagacca tg
//
LOCUS       DAOTKU010000168         3084 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_26080_length_3084_cov_3.298778, whole genome shotgun sequence.
ACCESSION   DAOTKU010000168 DAOTKU010000000
VERSION     DAOTKU010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3084
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26080_length_3084_cov_3.298778"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="P5310_04850"
     CDS             complement(<1..741)
                     /locus_tag="P5310_04850"
                     /inference="COORDINATES: protein motif:HMM:NF015647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HRU32722.1"
                     /translation="MLEELRAKNSRVIIGLMSGTSGDGVDAGVVRISGHWKDSEIKLI
                     HHYHYEYPEEMKRLIHKLFDFDNTSIHFLSHMNFLLGEVFAEVALETIKASKLSEKEI
                     DIISSHGQTVFHSPYPEDIGGFKVRSTLQIGEGAVIAERTGITTICDFRVRDMAAGGQ
                     GAPLVPFADWILFSSQKGNRCILNIGGIANVTILPEGGDIDDIIAFDTGPGNMVIDEI
                     IRIISSGLSDYDKDGAIAGKGKVNKQLLE"
     gene            complement(743..1105)
                     /gene="acpS"
                     /locus_tag="P5310_04855"
     CDS             complement(743..1105)
                     /gene="acpS"
                     /locus_tag="P5310_04855"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HRU32723.1"
                     /translation="MLGIDMVNVERIESAIKRRERFLYRVFSPEEIEEVKNRKKFYEA
                     LAGKFACKEAVIKALGGGLSPKDISVMHREDGSPYIILSDRARKKFESFMVNSIVVSI
                     SHDGGFAIAVAMLEKGVI"
     gene            1150..2190
                     /locus_tag="P5310_04860"
     CDS             1150..2190
                     /locus_tag="P5310_04860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HRU32724.1"
                     /translation="MDRRIIVNITTALLISISVIAIISSFMGEEVSVNEILDREVIWL
                     ILLCLAISIGSILLDGLRICIVVSALREKIRYIDAVESCLMYSFFSAITPSSMGGQPF
                     QIYFLAKKGIKSEAAANIALFRTFEYLLVVLGIDIYSVIFIIPKLSNWSVGKTMIILG
                     FASSVFSTGLTWITMKKPEIYKYLIAKLKRIKKLTNFIERWEDKTYTWIDSLKLSVNS
                     LLKNKKAVALDLFLMLISVLLYSYILFLPVIRLTGIKINFLTFFSVQTLLSSLATYIP
                     TPGASGGLEVILYSSFKSFVVSPRSLLLGITIYRIATYYIIILGGIPLILKNQKLLWR
                     EKGSAGDTDTSA"
     gene            complement(2207..>3084)
                     /locus_tag="P5310_04865"
     CDS             complement(2207..>3084)
                     /locus_tag="P5310_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32725.1"
                     /translation="AVLSLVIPIVYQVSEGVVFSTAAASVTNELKLINQKLIQSIKSD
                     VVQSAVVYDSYTSIIDLNLPTNYTSLNKNKLPVINETGSFPPEPDKVGNILFMAKYLS
                     PVEITVNGTTYRIDCYRFICYFLAKDTGSTINGKNPIILMRSQSQETYVDYSASKQKD
                     LVKALYNTGIRHAIDLKNTNFYALNSNGNINSEDNHTVQMESNPASRDFGANQLPTGK
                     VYYAIGYNNMGLIDIPKFASPDNSGDGFPNGFEVAIVGPKSSRDILVRTVVIGYFSGK
                     VLGNENTTIISVPQF"
BASE COUNT          896 a          657 c          511 g         1020 t
ORIGIN      
        1 ttccagtaac tgtttattta ccttcccctt tcctgctatg gctccatcct tatcataatc
       61 tgacaatcca ctggaaataa tccttattat ctcatctata accatattac ctggtccggt
      121 atcaaatgct atgatgtcat ctatatcccc tccttcagga agtatggtta cattggctat
      181 cccaccaata ttaagtatgc atctattgcc cttctgagaa gaaaaaagaa tccagtcagc
      241 aaaagggacg agtggtgcac cctgcccacc agctgccata tctctcactc taaagtcaca
      301 tatggtggtt atccctgtcc tttcagctat tacagcccct tcccctatct gcagagtaga
      361 tctaacttta aaacctccaa tatcttcagg atagggagaa tgaaaaaccg tctgcccatg
      421 actggagata atgtcaatct ccttttcaga cagttttgat gccttgatgg tctctaacgc
      481 cacctcggca aagacctctc ccagtagaaa attcatatga gagagaaaat ggatactggt
      541 attatcgaag tcaaacaatt tatgtatcag tctcttcatc tcttccggat actcatagtg
      601 ataatgatgg ataagtttta tctccgaatc tttccagtgt cccgatatcc ttactacacc
      661 tgcatctaca ccatctccgg atgtccctga cataagcccg attattactc tggaattttt
      721 agccctcaat tcttccagca ccttatatta cccctttttc cagcatcgct accgctatgg
      781 caaatccacc atcgtggctt atactgacca ctatgctgtt caccataaaa ctttcaaact
      841 tttttctagc cctgtcgctg aggataatat atggggagcc atcctctcta tgcatgacag
      901 agatatcttt aggggataat cctccaccga gtgcttttat taccgcctct ttacaggcaa
      961 atttaccagc gagagcctca tagaattttt tacgattctt gacctcttca atctcctcag
     1021 gggagaagac tctatagaga aatctctctc tcctttttat cgctgactct attctctcta
     1081 cattcaccat atctattccc aacataacat ctactattat acaacaatta gatgttataa
     1141 taataatcta tggatcgcag gattatagta aatattacta cagctttact aatcagtatc
     1201 tctgttattg cgattattag ctcttttatg ggagaagaag tttctgtaaa tgagatactg
     1261 gatagagaag tgatatggct aattctcctc tgtttggcta tcagtatagg ctctatactt
     1321 ctagatggac ttagaatatg tatagtggta tcagccctca gagagaagat cagatatata
     1381 gatgctgttg aatcctgtct tatgtactcc tttttttctg ccataactcc ctcttcaatg
     1441 ggagggcaac cctttcaaat ctattttttg gcaaaaaaag ggataaagag tgaagctgca
     1501 gctaatatag cactattcag gacatttgaa tatctgttgg tagtattggg gatagatata
     1561 tattcggtaa tattcataat tcccaaattg tccaactggt ctgttgggaa aacgatgatt
     1621 atacttgggt ttgccagtag cgttttttct accggtctta cctggattac tatgaaaaaa
     1681 cctgagatat ataagtatct catagctaaa ctgaaaagga taaaaaaatt aacaaatttt
     1741 atagaaagat gggaagataa aacttatacc tggatagata gtctcaaact cagtgtaaac
     1801 tccctattaa agaataagaa agcggtagct ctagatcttt ttctgatgct gatttcagta
     1861 ctcctctatt cttatatact ttttcttccc gtcataagac ttacaggtat aaagataaat
     1921 tttcttactt tcttcagtgt ccagaccctt ctctcctctc ttgctactta tataccgact
     1981 ccaggagcaa gcggaggttt agaggtaata ctctacagct cctttaagag ttttgtggtc
     2041 tctccaagat cactgcttct ggggataact atatatagaa ttgcaacata ttacatcatt
     2101 attcttgggg ggataccgct aatactcaaa aaccaaaagt tactctggag agagaagggg
     2161 agtgctggcg acactgacac ctctgcttga gggcaagaca gttttatcag aactgcggga
     2221 cacttataat ggtagtattc tcattaccaa gcaccttacc tgaaaaataa ccaattacta
     2281 ctgttcttac cagtatatct ctgctcgatt ttggacctac tatagccacc tcaaagccat
     2341 ttgggaatcc atcaccactg ttatcagggc tagcaaactt tggtatatct attaatccca
     2401 tattgttata acctatagca taatagactt tcccagttgg aagttgatta gctccaaaat
     2461 ctctggatgc aggatttgac tccatctgga ctgtatgatt atcttcagag ttgatattcc
     2521 cattactatt taatgcataa aaatttgtat tcttcaaatc aatagcgtgt cttatacctg
     2581 tattatatag agcttttacc aggtcttttt gtttggatgc actataatcc acataggttt
     2641 cttgtgattg acttctcata agtattatcg gatttttccc atttattgta gaacccgtat
     2701 cttttgctaa gaaatagcat ataaatctat agcaatctat tctataggtg gtaccattta
     2761 cagtaatctc tacaggactc agatattttg ccataaagag tatattcccc actttatctg
     2821 gttctggagg gaaactacct gtctcattta taactggaag tttgtttttg ttcaatgacg
     2881 tatagttagt tggcaaattt aagtctatta tactggtata gctatcatat actactgctg
     2941 actgaaccac atcagatttt atactctgta taagtttctg atttataagt ttcagttcat
     3001 tggtaacact ggcagcagct gttgaaaaaa ctacaccctc tgatacctga tatactattg
     3061 gaatcactaa agacaacact gcta
//
LOCUS       DAOTKU010000169         3081 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_26117_length_3081_cov_3.212492, whole genome shotgun sequence.
ACCESSION   DAOTKU010000169 DAOTKU010000000
VERSION     DAOTKU010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26117_length_3081_cov_3.212492"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="P5310_04870"
     CDS             <1..492
                     /locus_tag="P5310_04870"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HRU32726.1"
                     /translation="RKVVYAQSLLKPRKLEPIISYGTQLGVESFVLFISRRSSFKEID
                     AVRERIGRLKKLAASSAEISMNRIPEIYVIPDLISVLEKFSIYKPLLLYENAQERLNL
                     DWLKKNNLENMLVIVGPEGGFEGEEVETVRKSGGSILSLGDRIVTSEVAGFVALSLIQ
                     FGV"
     gene            499..1737
                     /gene="mtaB"
                     /locus_tag="P5310_04875"
     CDS             499..1737
                     /gene="mtaB"
                     /locus_tag="P5310_04875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HRU32727.1"
                     /translation="MKFRIYTFGCKTNQYDSEFLRRNFLQLMKEADSDEKPDLIVVNT
                     CSVTHIAERKARTLIRHLLNTYPEAKIIVTGCYAERSPDELERLGDIRVIGNMDKDKI
                     LDLFSINSSISSPDAGKGRRIRAFVKIQDGCDDFCSYCIVPYVRGPVRSRSFEEIIGE
                     IEQLVKFGYLEIVLTGIHIGKFESDGKSLPDLVRTISDNFPEIRRIRLSSLEPQEVNR
                     DIIDLFKDVSNLCPHIHLVAQSGSNKVLRDMKRKYTREEFLSIIDDFRSVMKNIEFTT
                     DIIVGFPTEEEEDFRDTISLIEEVGFVKVHIFPFSPRPGTEAFDLTPLDKKIVKEREE
                     LLKAVSLETAFNKLNKLVGNYVEILVERYSDGMAAGLTPNYVRVYLNTIEPLKPGEII
                     KVKVNGVEKAKDSVVLRGVY"
     gene            1809..2468
                     /locus_tag="P5310_04880"
     CDS             1809..2468
                     /locus_tag="P5310_04880"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HRU32728.1"
                     /translation="MDTRFKNIAVIGLGRFGFSLAVNLQQLGCDVLAIDSDPNVVEDI
                     KDSISRALVLDATDRIALEEAGIGDMDAVCISMGRDLQSSVLAALIVKKMGIGYILAT
                     AINDLHRKILEKIGVNLIISPEKDMGQRIAYRLTHPNILDRIEVSPDIEMIEIDPLDW
                     MIGKTMSEIGLPRKFGIRVFAIKRGNITMTEISGDTRIEKGDKIYIYGKHNVLERFAE
                     H"
     gene            2470..>3081
                     /locus_tag="P5310_04885"
     CDS             2470..>3081
                     /locus_tag="P5310_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012582679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HRU32729.1"
                     /translation="MQRETNPWRFIVLSFLLIILVGTIFLMLPVSSQSGKFTDPITAL
                     FTATSATCVTGLVVVDTGTYWSLFGQIVILLLIQLGGISYMTLLSFLALLLRRQVVLH
                     ERIILQETLNSWSIRGVMKLARVVLYTVVFFEGLGALLLFFVFIRDYPFITALKFSIF
                     HSISAFCNAGFDLLGGFSSLTGYVDNPLLVFTITSLIIFGGIGF"
BASE COUNT          949 a          467 c          707 g          958 t
ORIGIN      
        1 cgaaaagtgg tatatgcaca atctcttttg aaacctagga agttagaacc aataatctct
       61 tatggaacac aactcggagt agaaagtttt gttttattca tctcaagaag gtcttctttt
      121 aaagagatag atgctgtaag agaaaggata ggaagattaa agaagttagc tgcatcctca
      181 gctgagatct ctatgaacag gattccagag atatatgtaa tacctgatct tatctcagtt
      241 ctggagaaat tcagtattta taagcctctc cttttatatg agaatgccca ggaaaggttg
      301 aacttggatt ggctcaaaaa gaataatttg gaaaacatgc tggttattgt aggacctgag
      361 ggtggatttg agggagaaga ggtagagaca gttagaaaaa gtggcggtag cattctgtct
      421 ttaggggaca gaatagtaac atcagaagtc gcaggttttg ttgcactctc tcttatacag
      481 tttggagtgt aattaaatat gaagttccgt atatatacgt ttggttgtaa aactaatcag
      541 tatgactcag aatttctcag gagaaatttt ctccagttga tgaaagaggc tgattctgac
      601 gagaaaccag atctcattgt tgttaatacc tgttcggtga ctcatatagc tgagaggaaa
      661 gccaggacac ttataagaca tcttttaaac acatatcctg aagcaaaaat aatcgttact
      721 ggatgttatg cagagagaag tcctgatgag cttgaaagac ttggtgatat tagagtaatt
      781 ggaaacatgg ataaagataa gatactagat ttattctcta tcaattctag tatctcatct
      841 ccagatgctg gtaaaggaag aagaatacgt gcttttgtaa agattcagga tggatgcgat
      901 gatttttgct catactgcat agtcccatat gtcaggggtc ctgtccgatc gaggagtttt
      961 gaggaaatca taggagagat tgaacagctg gtcaagtttg gataccttga gattgttttg
     1021 acagggatac atataggtaa gtttgaaagt gatggtaaga gtctccccga tctggtaagg
     1081 actatatcgg ataatttccc agagataaga agaataagat tgagttcttt agagccacag
     1141 gaggtaaaca gggatataat agaccttttt aaggacgtca gcaatctgtg tcctcacatc
     1201 catctcgttg cacagagcgg aagtaataaa gtccttagag atatgaagag aaaatatacc
     1261 agagaagaat tcctctccat tatcgatgat ttcagatccg ttatgaaaaa cattgaattt
     1321 accaccgata ttattgttgg tttccctact gaagaagaag aggatttcag ggacactatt
     1381 tctttaatag aagaggttgg ttttgttaaa gttcatatat tcccattttc tcccagacct
     1441 ggaacagagg cttttgacct gaccccactt gataaaaaaa tagttaaaga gagagaagaa
     1501 ctgctgaagg cggtgagttt agaaactgca tttaacaagt taaacaaatt ggtaggtaat
     1561 tatgtggaga ttctagttga gagatattca gatggtatgg ctgctggttt aaccccgaat
     1621 tatgttagag tttatttaaa tacaatcgaa cctttgaagc caggggagat tataaaagtc
     1681 aaggtcaatg gtgtagaaaa agctaaggat agtgtggttt taagaggagt atactgagat
     1741 aaatctctat ttgcctatat tgcttttcta gcttttagtg gtataatcct atttctgggt
     1801 gatatattat ggatacaaga tttaagaata tagctgtaat aggattaggt agatttggat
     1861 tttctctggc tgtaaatctt cagcagctgg gatgtgatgt gctggctata gatagtgacc
     1921 cgaatgtcgt agaggatata aaggatagca tatcgagagc cttagtatta gatgctactg
     1981 atagaattgc cctggaagag gctggaatag gcgatatgga tgcggtctgt atttcaatgg
     2041 ggagagactt acagtcaagt gttctggctg ctcttattgt aaaaaagatg gggatagggt
     2101 atatcttagc tactgctata aacgatttac atcgaaagat ccttgagaag ataggtgtaa
     2161 atctgattat atctccagaa aaagatatgg ggcagagaat cgcttacaga ttgactcatc
     2221 ctaacatctt agatagaata gaagtttcac ctgatataga gatgatagag atagaccctc
     2281 tggactggat gattggaaag actatgtcag agataggact tcccagaaag tttgggataa
     2341 gggtctttgc tataaaacgt gggaatatca cgatgacaga aatctccgga gacacaagaa
     2401 tagaaaaagg ggataagata tatatctacg gaaagcataa tgtcctggag agatttgcgg
     2461 agcattgata tgcagagaga aactaatccc tggcgcttca tagttttaag ttttttatta
     2521 ataatccttg ttggaacaat ttttttaatg ttgccagtct cttcccaatc tggaaaattc
     2581 accgatccta ttaccgctct ttttactgcg acttctgcca cctgcgtcac cggacttgtg
     2641 gttgttgata cagggacgta ctggtctttg tttggacaga tagttatcct tttgttgata
     2701 cagctgggtg gaataagcta tatgaccctc ctgagttttt tagccctgct tcttcgaaga
     2761 caggtggttc tccatgaaag gattattctt caggaaaccc tcaacagttg gtctataaga
     2821 ggggtaatga aattggctcg agtagtctta tataccgtag tcttctttga aggtcttggg
     2881 gcgttattgc tattttttgt atttataagg gactatcctt ttataacagc attaaagttt
     2941 agtatcttcc acagtatttc tgctttctgt aatgcaggtt ttgatcttct tggaggattc
     3001 agtagtctta ctggttatgt ggataatcct ctcctggtat ttaccataac ctctttaata
     3061 atctttggtg gaatagggtt t
//
LOCUS       DAOTKU010000170         3056 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_26378_length_3056_cov_3.160280, whole genome shotgun sequence.
ACCESSION   DAOTKU010000170 DAOTKU010000000
VERSION     DAOTKU010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26378_length_3056_cov_3.160280"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            208..1497
                     /locus_tag="P5310_04890"
     CDS             208..1497
                     /locus_tag="P5310_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012582824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HRU32730.1"
                     /translation="MHKWVSGILVLLMCLTVFSTILPAQEKISLRVAWWGSQDRHNRT
                     LKAIELFEKKYPNIKIYPEYTAWNDYWTKLNTQAAGKNLPDVIQHDYAYLSEWATRGL
                     VLPLDEYISKGVIDLSSVAENQISPGKIGGKVYGINLGTNSLGMLYDPELFKKAGIPF
                     PKVGWTWNDFREIATRLKNKLGIYGAEAITKADAQVFKVWLMSHGYWLYSEDGKSIGY
                     KDDKLFVDWFNLLLDLQSKGAIPSIETDIARGTLGVEDMFIVTQKSAMGLLWSNQIVA
                     VSKAAKERPLRFALMPRAPIPNAKPANYLKASQFFSVTSQSKHPKEAAMFIDFFTNSL
                     EANKVLLAERGVPISSKIQKSLLPFLGSSQREMFNFVSLAEKNSLPIPPPDPPGATDV
                     VNNVWNPIVEQILYGRITPDKAAVEFRDKVTKILQQR"
     gene            1575..2465
                     /locus_tag="P5310_04895"
     CDS             1575..2465
                     /locus_tag="P5310_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRU32731.1"
                     /translation="MNKRERYNNVMGYLFISPWLIGFFIFTLFPIIASFILAFTRYDV
                     LSPPRWIGIDNWFRLLEDTRYHNSLKATFYYVFTAVPLRLIFALLVAMLLKPSTRLAG
                     LYRGIYYLPSLIGGSVAVAVVWRQIFGSEGLINFILGMMGIKGLNWLGNPSTAIWTLI
                     ILAVWQFGSPMLIFLAGLKQIPDSLYEAASIDGAKGWDQFTKITLPMLSPVILFNLIM
                     QIINGFMVFTQAYIITSGGPLDTTLFYSLYLYQKAFQSFEMGYASAMAWVLLMIIAFF
                     TALAFKSSRFWVYYESEEGK"
     gene            2474..>3056
                     /locus_tag="P5310_04900"
     CDS             2474..>3056
                     /locus_tag="P5310_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015908433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRU32732.1"
                     /translation="MAKKAKRQNIIYHIATFILGIIMIYPLLWMISSSFKENADIFTT
                     MGTLIPPKFTVENYIEGWKGFGNVSFATFFKNSFIVAGIGTIGTVISSAIVAYGFARI
                     PFKGKDLWFTAMMITLMLPFQVQIIPQYIMFNTLNWINTFYPLIVPKFFGSAFFIFLI
                     IQFIRGLPLELDESAEIDGAGRIGIFWWIILPLI"
BASE COUNT          979 a          541 c          644 g          892 t
ORIGIN      
        1 tttataataa taatcccatg cctttatcct cccgccctca tctcggggga ggatagaggt
       61 gggagagggg aataggtcag aactatttat ccatgacaat atcacagaac gataacacaa
      121 aaatattgac agtgaatgag tatatagtta taatataggc gattccatta acaatgtttc
      181 ataaaataaa acactaggag gtgttgtatg cataagtggg tttctggaat cttggttttg
      241 ttgatgtgtc taaccgtctt ttctacaatt ttaccagctc aggagaagat atctctgagg
      301 gtggcatggt ggggatcgca ggacaggcat aacaggacgc tcaaagctat tgaattattt
      361 gagaaaaagt atccaaatat aaaaatctac cctgaatata ctgcatggaa tgattactgg
      421 acgaagctca atacccaggc agcggggaag aatctccccg atgtaataca gcatgattat
      481 gcatatctat cagaatgggc tactcgaggt ctggtgttac ctctggatga atacatatct
      541 aagggagtaa tagacctctc aagtgtggca gaaaaccaga tatctcccgg gaaaataggc
      601 ggaaaggtct acggtataaa tctggggaca aattctctgg gaatgttata tgatccggaa
      661 ctattcaaga aggcaggaat accttttcct aaagtaggct ggacatggaa tgattttaga
      721 gagatagcta caagattgaa gaacaaatta ggtatatatg gagcagaagc gataacaaaa
      781 gcggatgcac aggtattcaa ggtatggcta atgtcacatg gttactggtt atactctgaa
      841 gatggaaagt caataggtta caaagatgat aagctatttg tagactggtt taatctatta
      901 ttagacctgc aaagtaaagg tgctatacca tcaatagaaa ccgacatagc cagagggacg
      961 cttggtgtag aagatatgtt tatagttact caaaagtctg caatgggatt gctctggagt
     1021 aaccagattg ttgcagtatc caaagcggca aaagaacgtc cattaagatt tgcgctgatg
     1081 cccagagccc ctattcctaa cgctaaacct gccaactact taaaggcttc acaatttttc
     1141 tccgtaactt ctcagtctaa acatccaaaa gaagctgcca tgttcataga cttctttaca
     1201 aattctttag aggcgaataa ggtattactt gctgaaagag gggttccaat atcatcgaag
     1261 atacagaagt ctctactccc gtttctggga tcatcccaga gagagatgtt taactttgta
     1321 agcctggctg aaaagaatag tcttcctatt ccaccaccag acccacctgg agctaccgat
     1381 gtggtaaata acgtatggaa cccaattgta gaacagattc tttatgggag aataactccg
     1441 gataaggcag ctgtagaatt tagagataaa gtaaccaaga tattacagca gagataaaaa
     1501 cctgtaaaac aaacccccag tagatgacct ctactggggg ataaacatca accgagaagg
     1561 tgaatgaata aaagatgaat aaaagagaaa gatataataa tgtcatgggt tatctgttta
     1621 taagtccctg gcttataggc ttttttatat ttacactatt tccaataata gcatcgttca
     1681 tccttgcttt caccagatat gacgtccttt ctcctccacg atggattgga attgacaatt
     1741 ggtttcgtct attagaagat accaggtatc ataactcctt aaaagcaaca ttctattacg
     1801 tttttacagc agtccctctg aggctgatat ttgcacttct ggtggcaatg ttattaaaac
     1861 cttcgacaag gttagcaggt ctgtacagag ggatttatta tctaccatca cttataggag
     1921 gaagtgtagc agttgctgtc gtttggagac agatatttgg atcagaaggg ctgataaact
     1981 ttatattagg gatgatgggg attaaggggt taaactggtt gggtaatccc agtactgcta
     2041 tctggacact tataatactg gctgtatggc agtttggttc ccctatgctg atctttctgg
     2101 caggattaaa acagatccct gattctcttt atgaagcagc atccatagat ggagctaagg
     2161 gatgggatca gtttacaaaa atcactcttc caatgctaag ccctgtaatc ttattcaacc
     2221 ttataatgca gataattaat ggctttatgg tatttacaca ggcatatata ataacctcag
     2281 gtggaccatt agataccacc ctattttatt cactatatct ctatcaaaag gctttccaga
     2341 gtttcgagat gggatatgca tctgctatgg catgggtatt gcttatgata atagcattct
     2401 tcactgcact ggcatttaaa tcatccagat tctgggtgta ctacgaatca gaagagggta
     2461 aataggaggt aaaatggcaa aaaaggctaa aagacagaat attatctatc atatagctac
     2521 atttatattg gggatcataa tgatctatcc tcttttatgg atgataagta gttcatttaa
     2581 agagaatgca gatatattta ccacaatggg aactcttata cctccaaagt ttacggtgga
     2641 aaactatata gagggatgga aggggtttgg aaacgtaagc tttgcaacat tctttaaaaa
     2701 ctcatttata gtagctggca taggcaccat aggtacagta atctcctctg caattgtagc
     2761 atatggattt gctaggatac cgttcaaagg aaaagatcta tggtttacag ctatgatgat
     2821 aacattaatg ttaccattcc aggttcagat aattccccag tatataatgt ttaataccct
     2881 gaactggatt aacacattct atcccttaat cgttccaaaa ttttttggct ctgcattctt
     2941 tatctttctt ataatacaat ttataagagg attaccactg gaattggacg agtctgcaga
     3001 gatagacgga gctgggagga ttggaatctt ctggtggatt atcttgcctt taataa
//
LOCUS       DAOTKU010000171         3051 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_26435_length_3051_cov_3.972630, whole genome shotgun sequence.
ACCESSION   DAOTKU010000171 DAOTKU010000000
VERSION     DAOTKU010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26435_length_3051_cov_3.972630"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..1581)
                     /gene="glpK"
                     /locus_tag="P5310_04905"
     CDS             complement(94..1581)
                     /gene="glpK"
                     /locus_tag="P5310_04905"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013782162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HRU32733.1"
                     /translation="MERYILSIDQSTSSTKAIIFNSVGKLVHRFNIEHKQYYPKPGWV
                     EHNPEEIYENTLIAINKVIQESGVAIEDIAALSITNQRETAVVWSKKTGKPVCNAVVW
                     QCQRSSEICKKLEREGLQKTIKEKTGLVLSPYFSASKIKWILDNIESARERAESGELL
                     CGTIDSWLIWKLTNGENHLTDYTNASRTQLFNIRELSWDKELLEIFTIPESMMPMVKK
                     SDEIFGYFSLGNKIRRSIPVTGITGDSHAALFGQACFEKGMAKATYGTGSSVMMNIGD
                     RLVIPASGIVTSIGWNTKDGISYVLEGNINYTGATIKWLVENLELLANSKEAGIIAST
                     VDDNNGVYFVPAFVGLGAPYWDSDARAIITGMSSNTRKAHIIRAAEESIAYQIRDIIE
                     LMTEESSITLKELRVDGGPTRDNFLMQFQADILNVPVVRVKIEELSALGSAFIAGLAT
                     GIWNNPDELTSLREIDRVFIPEMSSAERERLYGGWKQAVKLSLSE"
     gene            complement(1588..3045)
                     /locus_tag="P5310_04910"
     CDS             complement(1588..3045)
                     /locus_tag="P5310_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019006319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5605 domain-containing protein"
                     /protein_id="HRU32734.1"
                     /translation="MEESLMKVERWGIFETTLNGPSEGNPFLDISIRAEFKYKNKKVE
                     VEGFYDGDGIYKIRFMPDSEGEWSFITASNINSLDRIEGKFLCIPPSQNNHGPVRVVN
                     TFHFAYEDGTPYYPIGTTCYAWAHQGEELEKQTLDTLKKSPFNKLRMCVFPKHYEFNH
                     NEPEYYPFEGSPERGWDFSRFNVEFFRHMEVRIKDLQDLGIEADMILFHPYDRWGFST
                     MDGEFDDRYLKYVIARFSAFRNVWWSLANEYDIMRSKTLLDWERFARIIRENDPYGHL
                     RSIHNCRVMYDFTKPWVTHCSIQRVDWYKTAENTDTWRETYKKPVVIDECAYEGNIHR
                     GWGSITGEEMVRRFWEGYVRGGYVGHGETYLHPEDILWWSKGGKLYGKSPERIAFLRR
                     IIEEGPNYGINPIHMYTDIPCGGIEGEYYIIYLGFNQPAYRIFKLPSGIRFKVEIIDT
                     WDMTVEEVPGIYEGEFKICLPSKPYIAIRLKQIKE"
BASE COUNT          801 a          714 c          481 g         1055 t
ORIGIN      
        1 tataagtgag tagaaaaaga gataactgcc agatctattg aattgaccca agtcaaatat
       61 agagagaccc aagtattcag tgggtctctc ttcttactca ctgaggctaa gttttactgc
      121 ctgcttccat ccaccgtata gtctttctct ttccgctgaa ctcatctctg gtataaatac
      181 cctgtctatc tctctcagag atgtaagttc atctggatta ttccatattc ctgttgccag
      241 accggcaata aatgctgatc caagtgctga tagttcctca attttaaccc tgactaccgg
      301 aacatttaaa atatctgcct gaaattgcat tagaaagttg tctcttgtag gtccaccgtc
      361 aactctaagc tcttttaaag ttatgcttga ttcttctgtc atcaactcta taatatctct
      421 tatctgataa gctattgatt cttcggcagc ccttataata tgcgcctttc tggtatttga
      481 tgacatacct gtaattatcg ctcttgcgtc actatcccaa tatggtgctc ctaatccaac
      541 gaatgcaggg acaaagtata cgccattgtt atcatccact gtacttgcta taattccagc
      601 ctcttttgaa ttagcaagaa gctctagatt ttctaccagc cactttattg tggcaccggt
      661 ataatttata tttccctcca gaacataaga aattccatcc ttagtattcc agcctataga
      721 tgttactata ccgctggcag gaattaccaa tctatctcct atattcatca ttacagaaga
      781 gccagtgcca tatgttgctt tagccatacc cttttcaaaa caggcttgac caaagagtgc
      841 agcatgggag tctcctgtga ttcctgttac aggaatactt ctcctgatct tgtttcctag
      901 tgaaaaatat ccaaatatct catcagattt tttcaccatg ggcatcatag attctggtat
      961 agtaaatatc tctaaaagtt ctttgtccca gcttaattct ctaatattga acagctgggt
     1021 acggctggca ttggtgtaat cggtcaggtg gttttcacca tttgtaagtt tccatataag
     1081 ccaggaatca atagttccac atagtaattc tcctgattca gccctctctc tggctgattc
     1141 tatattatcc aggatccact ttattttgga tgctgagaag taaggtgata agactagtcc
     1201 ggttttttct tttatagttt tctggagacc ttccctctct aactttttac atatctctga
     1261 gctacgctga cattgccata caacagcgtt gcataccggt tttccggtct ttttactcca
     1321 gaccacagcg gtttctctct gatttgttat agataatgca gctatatctt ctatagccac
     1381 cccactttcc tggatcactt tattaatagc tataagtgta ttttcatata tctcttcagg
     1441 attgtgctct acccaacctg gctttggata atattgctta tgctcaatat taaatctgtg
     1501 aaccagtttt cccacactgt taaaaataat tgcttttgta ctggaggtgc tctggtctat
     1561 agataggata tatctttcca tagaaaccta ttccttaatc tgtttaagcc ttattgcaat
     1621 atagggcttt gatggaagac atattttaaa ctcaccctca tatatcccag ggacttcttc
     1681 tactgtcata tcccaggtgt ctataatctc aaccttgaat cttatcccgg aaggtagctt
     1741 aaatattcta tatgctggct gattaaaacc aagatagata atataatatt ctccctctat
     1801 cccaccacat ggtatatcgg tatacatatg tatcggattt atcccgtaat ttggtccttc
     1861 ctctattatt cttcttaaaa atgctattct ctctggactc ttgccgtata acttcccacc
     1921 ttttgaccac cacagtatat cctctggatg gagatatgtt tcaccatgcc ctacatatcc
     1981 acctctaaca tatccctccc agaatctcct taccatttct tcgccggtta tgcttcccca
     2041 tcctctatgt atatttcctt cataggcaca ttcatctatc accacaggtt ttttgtatgt
     2101 ttctctccag gtatcagtat tctcagcagt tttataccag tccactctct gaatgctgca
     2161 gtgggttacc catggttttg taaaatcata cataactcta cagttatgta tggacctcaa
     2221 atgtccatag gggtcatttt cccttattat tctggcaaat ctctcccaat ccaacagggt
     2281 tttggatcgc atgatgtcat actcgtttgc cagcgaccac catacatttc tgaatgcaga
     2341 aaaacgggct attacatatt tgagatatct atcatcaaat tctccatcca ttgttgagaa
     2401 accccagcgg tcataaggat gaaagagtat catatctgcc tctattccca aatcctgcag
     2461 gtcctttatc cttacctcca tatgcctgaa gaattcgacg ttaaacctgc taaaatccca
     2521 acccctctct ggagagcctt caaagggata atactcaggc tcattatggt taaactcata
     2581 atgttttggg aatacacaca tcctcagttt attaaatggt gattttttca gggtatccag
     2641 tgtctgtttc tcaagctctt ctccttgatg tgcccaggcg taacatgttg ttcctattgg
     2701 ataataaggc gttccatctt cgtaggcaaa gtgaaatgta tttacaaccc tcacaggtcc
     2761 gtggttattt tgagatgggg gtatacataa aaatttgcct tctatcctgt ccagtgagtt
     2821 gatgttactt gctgttataa aactccattc tccttctgag tcgggcataa atcttatctt
     2881 atagattcca tctccatcat aaaaaccttc aacttcaacc tttttatttt tgtatttgaa
     2941 ttctgccctt atactgatgt ctaaaaaggg attcccctca gatggtccat ttaatgtagt
     3001 ttcgaatatc ccccacctct caactttcat aagactttct tccatattaa c
//
LOCUS       DAOTKU010000172         3023 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_26718_length_3023_cov_3.004717, whole genome shotgun sequence.
ACCESSION   DAOTKU010000172 DAOTKU010000000
VERSION     DAOTKU010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3023
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26718_length_3023_cov_3.004717"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            228..1376
                     /locus_tag="P5310_04915"
     CDS             228..1376
                     /locus_tag="P5310_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRU32735.1"
                     /translation="MKRLFALVIMLSLILGSIVQVYGAETIKIGLVTPLTGSVASFGQ
                     SVDKAVRMAVDEINSKGGLLGMKIELIVEDNQSKAEESANIVRKFIEQDKVVAILGPV
                     ISSNTLAAAPIAQQSKVPLLSPTSTNPKVTQIGDYIFRACFIDDFQGMVMARFAMTGL
                     GKRPSSAAILFEKTSDYSIGLAKYFKEEFTRLGGKIVAEESFSSGDQDFSAQLTKIKG
                     RKPDVLYVPSYYDTAGLIIKQARELGMNIPILGADGFDSPQLAELAGKENLKNCYFSG
                     HFFSGSSAPQVKTFVTNHKKRYNTVPDMLGALGYDAVYMLADAIKRAGKVDREAIKNA
                     LATTKNLKLVTGTISLDENRNPIKSAVIISFDANGNQVYKTTVNPPKK"
     gene            1437..2327
                     /locus_tag="P5310_04920"
     CDS             1437..2327
                     /locus_tag="P5310_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008409942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRU32736.1"
                     /translation="MVEFIQQMINGIALGSIYALIALGYTMVYGILRLINFAHGDIYM
                     VGAFAGYFAMTLFKWGIVPSFIFSMLVCALLGVSIERFAYKPLRNAPRIAVLTTAIGV
                     SFLLENLGVIFWTPTPRAFPQVIPLKIYQGIYERFGIIINNRQVVIVSISIALMILLQ
                     FIVNKTKIGMAMRAVSSDRETAWMMGVNIDHVISFTFAIGSMLAAAGGVLVGIYFNRI
                     DPLMGVMPGIKAFVAAVLGGIGIIPGAMVGGFILGIAEILVSAFIASSYRDAIAFAIL
                     ILILLVRPSGIFGTAAREKV"
     gene            2331..>3023
                     /locus_tag="P5310_04925"
     CDS             2331..>3023
                     /locus_tag="P5310_04925"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRU32737.1"
                     /translation="MERLKNLRYLLIVIGILVIYGIGLLSPNISPYYLQIIIFSGINV
                     ILAVSLNVINGLCGQFSLGHAGFMAVGAYFSSFLTFYYRVPFPIALILGGILAGIVGL
                     GVGIPTLRLRGDYLAIATLGMGEIIRVILFNLDIVGGARGFPGIPKHTNFFWVYLAVA
                     AVLVSSYNLLRSPHGRAILSIREDEIASEAMGVDTTRFKIFAFIFGSFWAGVAGGLWA
                     HYLQFLHPSSFTF"
BASE COUNT          885 a          522 c          685 g          931 t
ORIGIN      
        1 aatctgtatc ccgtaggggc aattcatgaa ttgcccctac aatttcgtct tttatgataa
       61 taatctcgca cctctattct ttgacgcgtc acggtattta tgctacctat cacgagttac
      121 gtatccatac ccgacaatac cacagaacgt taacacaaat tgttgacaat tatataaacc
      181 tctagtagaa ttatagacaa atttaatcca taatggaggt gatttttttg aagaggttgt
      241 ttgcacttgt aataatgctt agcctaattt tgggcagtat cgtacaggta tatggagcag
      301 aaactataaa gataggactt gttactccac ttacaggttc cgtggcatca tttggtcaat
      361 ctgtagataa ggctgtaaga atggctgttg atgagattaa ctctaagggt ggattactag
      421 gtatgaagat agagttaata gtagaggaca atcagtcaaa agcagaagaa tcggccaata
      481 ttgtccgtaa gtttatagag caggataaag tagtggctat actgggtcct gtaatctcaa
      541 gtaatacatt agcagctgcc cccatcgcac agcagagtaa agttccactc ttgtcaccca
      601 catcaacaaa cccgaaagtt actcaaatag gagattatat cttcagggct tgttttattg
      661 atgacttcca gggtatggtg atggccaggt ttgcaatgac tggtcttgga aagagaccta
      721 gttcagcggc tatactcttt gaaaaaacga gcgactatag tattggatta gccaagtatt
      781 ttaaagagga atttacaagg cttggtggga aaatagtggc agaagaatcg ttcagctctg
      841 gagaccagga cttcagcgcc cagcttacca aaataaaagg tagaaaacca gatgtactct
      901 atgttccatc ttattatgat accgccggac ttataataaa gcaggcgaga gaattaggca
      961 tgaatatccc aatactgggt gcggatggtt ttgattctcc tcaacttgct gaacttgccg
     1021 gtaaggaaaa cctgaagaac tgctatttca gtggacattt cttctcaggt tcaagtgctc
     1081 cgcaagtaaa gacatttgtg acaaaccaca agaagaggta taatactgta cctgacatgt
     1141 taggtgctct gggatacgat gctgtctata tgctcgcaga tgctataaag agagcaggaa
     1201 aagtagatag ggaggctata aaaaatgccc tggcgactac taaaaatctg aagttagtaa
     1261 ctggaacaat atctctagat gaaaatagaa atcccataaa gagtgctgtc attatcagtt
     1321 ttgatgctaa tggtaaccag gtttataaaa ctactgtaaa cccaccaaaa aagtagctca
     1381 aaaaaagtta agggcaggaa gaatttattc ttcctgccct taaaagggat aggaagatgg
     1441 tagaatttat tcaacagatg ataaatggaa tagcattggg aagtatctat gccctgatag
     1501 ctttagggta tactatggtg tatgggatat tgagacttat taattttgct catggagata
     1561 tatatatggt tggagcattc gctggctatt tcgctatgac attatttaag tggggaatag
     1621 ttccatcctt tatcttttct atgctggtat gtgctctatt aggagtaagt atagaaagat
     1681 ttgcatataa gcctcttaga aacgctccac gtatagcggt tttaacaacc gctatagggg
     1741 tttcttttct gcttgaaaat ttaggagtta tattttggac tcctacaccc agagcttttc
     1801 ctcaggtgat accacttaag atatatcagg gtatatatga aaggtttgga ataattataa
     1861 ataatagaca ggtagttatc gtatctatct ctatcgccct gatgattctt ctccagttta
     1921 tagtaaataa aaccaagata ggtatggcta tgagagcggt ctcatctgat agagagaccg
     1981 cgtggatgat gggagttaat atagatcacg taatatcatt tacttttgct ataggctcta
     2041 tgctggcagc tgctggtgga gttctggttg ggatttactt taacaggata gaccctctta
     2101 tgggcgttat gccaggtata aaggcatttg tagctgctgt tcttggggga ataggaataa
     2161 tacccggagc tatggttggt ggttttattt tggggattgc agaaattctt gtctcagcat
     2221 ttatagcatc ttcctataga gatgccatag cctttgctat acttatattg atactgctgg
     2281 taagaccttc cggaatattc ggaactgccg ccagagagaa ggtgtagcgt atggaaagat
     2341 taaagaatct cagatatctt ttgatagtta ttggaatact tgttatatat gggattggtc
     2401 ttttatcacc aaatataagc ccttattatc tgcagataat catattttcc gggataaacg
     2461 tcatacttgc agtaagtttg aatgtaataa atgggctctg tggtcaattc tctctggggc
     2521 atgctggctt tatggctgtt ggtgcttatt tctcttcttt tttaacattc tattatagag
     2581 tgccattccc catagccctt atactgggag gcatactggc tgggatagtt ggacttggag
     2641 ttggaatccc cacactgaga ttacgggggg attatttggc tatagccacg ctggggatgg
     2701 gagagataat aagagtaata ctgtttaatc ttgacatagt tggaggagcg agaggtttcc
     2761 cgggtatacc aaaacatacg aacttcttct gggtgtatct tgcagttgca gccgtcttag
     2821 taagttctta taatctttta aggagtccac atggtagagc catcctctcg ataagagagg
     2881 atgagatagc ttctgaagct atgggggtag atactaccag gttcaagatc tttgccttta
     2941 tctttggttc attctgggct ggggtagcag gaggactctg ggcgcactat cttcaatttc
     3001 tgcacccaag ttcatttaca ttt
//
LOCUS       DAOTKU010000173         3012 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_26807_length_3012_cov_3.035509, whole genome shotgun sequence.
ACCESSION   DAOTKU010000173 DAOTKU010000000
VERSION     DAOTKU010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26807_length_3012_cov_3.035509"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..208)
                     /locus_tag="P5310_04930"
                     /pseudo
     CDS             complement(1..208)
                     /locus_tag="P5310_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343704.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(325..1392)
                     /gene="pheA"
                     /locus_tag="P5310_04935"
     CDS             complement(325..1392)
                     /gene="pheA"
                     /locus_tag="P5310_04935"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013886950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HRU32738.1"
                     /translation="MIEEYRSIIDRIDKEILELLNKRASLVIEIGKEKLKDNKPFIDI
                     VREEIHLRELLSSNSGPLAEDSIKNIFREIISASRMLEGTLKVAFLGPEGTFTHLAAL
                     KRFGRETFYIPARSIADVFSLVDKGEVTLGVVPIENSTEGVVNYTLDMFMVSSLNITG
                     EIIIPISHNLIAREDVKEIKRIYSHPQAFGQCKEYLEKNFPNADLIEAVSTAKAVEIA
                     SRDNESAAIGSEIAAEKFGLRVILFKIEDDPNNRTRFLIIGKSDKGRRSGKDKTMVMF
                     STAHKPGSLYNALRCFAEHNINLTMIQSRPSREALWEFVFFAEFQGHREDEDVKRALE
                     SLSEVVWSYRIIGSYPEDELV"
     gene            complement(1385..2941)
                     /gene="cimA"
                     /locus_tag="P5310_04940"
     CDS             complement(1385..2941)
                     /gene="cimA"
                     /locus_tag="P5310_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HRU32739.1"
                     /translation="MIYVYDTTLRDGAQREGISFSVEDKLKITTRLDELGVHYIEGGW
                     PGSNPKDAAYFNKAKDLKLKNARIVAFGSTRRTDKVVSEDANIRALLEADTPSIAIFG
                     KSWGLHVTEVLHTTLEENLKLISDSVGYVKSKGKEVIFDAEHFFDGFKENPQYAIATL
                     KVAQEQGADWIVLCDTNGGNLPNFIADCIEEVKKYITAPLGIHAHNDSDLAVANTIVA
                     VQKGATQIQGTINGYGERCGNANLCSCIPIIQLKLNLKCLSDESLKKLFEVSHYVDDI
                     ANNPPVDWQPFVGSYAFAHKGGIHVNAILKNPLTYEHINPELVGNRRRILVSELSGKS
                     NILYKIQELGLDIDLSSLNVSNLLEKIKQLEYQGYEFEGAEASFELLVKEMLGIYKPP
                     FEVKGFRVFIDKIDDKVTCERTVKINVDGTLEHTASEGNGPVDALDTALRKALTKFFP
                     KISAIKLTDYKVRVLEGSQGTSATVRVLIESTNGKSTWSTVGVSPNILEASYQALVDS
                     LAYGLSFAND"
BASE COUNT          796 a          719 c          528 g          969 t
ORIGIN      
        1 tatctgaatc aaagttacct gactgatatc gtgaccatga aagtcctgca tatttagcca
       61 aagaggcttg gcctttgaat ctgttgatat taccgatttc cgaaaggata ccggcagcat
      121 aaacagggcc aacgcctttt acagatgtca gagtgtttgg tatagtctcc atcagtgagg
      181 agatagattt atcaagggat tttagctgtt ctatatattc cctggttaac tttaccccag
      241 tatttaatag gatggctcca tcctgtctga atataggata accaagtata tctcccggtt
      301 tagcccaggt tatctttatc cttctcatac cagttcgtct tcaggatatg aacctattat
      361 cctgtaggac catacaacct cgcttaacga ttccagagcc ctttttacgt cttcatcttc
      421 tctgtgcccc tgaaattcgg caaagaatac gaactcccag agggcttctc tggatggacg
      481 ggattgtatc atggtaagat tgatattatg ttcggcaaaa catcttaaag cattatacag
      541 agaacctggt ttatgagcgg tagaaaacat caccatagtt ttatctttac cgctgcgtct
      601 acctttatca ctcttaccta tgatcaggaa tcttgtcctg ttatttgggt catcctctat
      661 cttgaagagg ataaccctga gtccaaactt ttcagctgct atctcggaac ctatagcagc
      721 actctcatta tctctgctgg ctatctccac cgccttcgca gtgctgaccg cttctataag
      781 atctgcattt ggaaagtttt tctccagata ctccttacac tgtccaaatg cctgcggatg
      841 agagtatatc ctctttatct ccttaacatc ttctctggct ataagattat gagatatagg
      901 gataattatc tctcctgtaa tattcagaga agaaaccata aacatatcta aagtgtagtt
      961 tactacaccc tctgttgagt tttctatagg gactacaccc agagttactt ctcccttatc
     1021 aacaagagaa aaaacatcgg ctatacttct ggcaggtata taaaaggtct ctcgaccaaa
     1081 tctttttaat gctgcaagat gggtaaaagt cccctctggt cccaaaaagg caaccttcaa
     1141 tgtcccctcc agcattcttg aggcagaaat tatctccctg aagatatttt taatactatc
     1201 ctctgctaga ggtccactat tagaagataa gagttctctc aggtgaatct cttctcttac
     1261 tatatctata aatggcttat tgtctttaag cttctcttta cctatctcta taaccaaact
     1321 ggctctctta tttagtagtt ccagtatctc tttatctatc ctgtctatta tagaccgata
     1381 ctcttcaatc attggcaaaa ctcaatccat aggccaggct atcaacaaga gcctgatagc
     1441 tggcttcaag gatattaggc gatacaccta ctgtgctcca ggtactttta ccattggtgg
     1501 attctatcag aactcttacg gtagccgatg ttccctgaga accttcgagg actctaacct
     1561 tataatcggt aagttttatt gcagagatct tggggaaaaa ttttgtcaat gcctttctca
     1621 atgccgtatc cagtgcatcc acggggccat tgccttcact ggcagtatgc tctaatgtac
     1681 catcaacatt tatcttgaca gttctctcgc atgtcacttt atcgtctatc ttatctatga
     1741 atactctgaa gcctttcacc tcaaatgggg gtttatatat acccagcatc tcttttacca
     1801 gtaactcgaa actagcttca gcgccttcaa actcataacc ctgatactct aactgtttta
     1861 tcttttccag cagattgctc acattcaggc tgcttaaatc tatatctaac cccagttcct
     1921 gtatcttgta gaggatattg gattttcctg agagttcaga gaccagtatc ctccgcctgt
     1981 tacccaccag ttccggattt atatgctcat atgttaatgg attcttcaga atggcgttta
     2041 catgtatccc acccttatgg gcaaacgcat aagatccaac aaatggctgc caatctaccg
     2101 gaggattatt tgctatatcg tcaacgtaat gagagacctc aaaaagcttc tttagagact
     2161 catcagatag acacttaaga ttgagtttta actgaataat aggaatacag gagcagagat
     2221 tggcattacc acacctctcc ccataaccat ttattgtccc ctgtatctgg gtagcaccct
     2281 tctgtacggc tactatggta tttgcgacag ccagatcact gtcattatgg gcatgtatac
     2341 ccagaggagc tgtaatatat ttttttactt cctctataca gtcggcgata aagttcggga
     2401 ggttgcctcc atttgtatca cagagaacaa tccagtctgc tccttgctcc tgtgccacct
     2461 tcagtgttgc tatggcatat tggggatttt ctttaaatcc atcaaagaaa tgctcggcgt
     2521 cgaatattac ctctttaccc ttagatttta cataacctac cgagtctgat atcaacttca
     2581 gattctcttc aagtgtggta tgtaaaactt ctgttacatg gagtccccag ctcttaccga
     2641 atatagcaat acttggagta tcggcttcaa gtagagccct tatgtttgca tcctcagata
     2701 cgaccttgtc agtccttctc gtactgccaa atgcaactat tctggcgttt ttcaacttga
     2761 ggtctttagc tttattaaaa tatgcggcat ccttaggatt ggaaccaggc catccacctt
     2821 ctatataatg gacgcccaac tcatcaagtc tggtggtaat ctttagctta tcctctacgc
     2881 tgaaagagat cccctcacgt tgagctccat ccctgagagt ggtgtcatat acatatatca
     2941 tcgcgcatca ccttctaaca tttccagtgt cttttttact atcagatctc ccatctcttt
     3001 tgtaccaacg at
//
LOCUS       DAOTKU010000174         3006 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_26866_length_3006_cov_2.960691, whole genome shotgun sequence.
ACCESSION   DAOTKU010000174 DAOTKU010000000
VERSION     DAOTKU010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3006
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26866_length_3006_cov_2.960691"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="P5310_04945"
     CDS             complement(<1..587)
                     /locus_tag="P5310_04945"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HRU32740.1"
                     /translation="MNLKPIALQMYTVRDYAEKNFIETVKKVAEIGYKGIELAGTYGV
                     KSDELKQILDSLDLKVCGSHIPIDLLEKDFDNTVKFNLELGNKNIVCPWLPEDRRNSI
                     EEWKKTAEELNSIGERLRADGLTLSYHNHNFEFEKFGDKYGIEILIENTDPENLKLEV
                     DVFWIKFAGLDPAEFLKKYIDRINLIHLKDMKADK"
     gene            976..>1298
                     /locus_tag="P5310_04950"
     CDS             976..>1298
                     /locus_tag="P5310_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010865258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32741.1"
                     /translation="MRRIFTVVLVFVLLISVVPISFSQEAKFHKQYSTPTDYYKATGK
                     RINKYSESPVLAEMVRQGKLPPVKERLPKEPIVISPVEEIGQYGGKAQVIGPVNSYGD
                     TEALRP"
     assembly_gap    1299..1398
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1399..>3006
                     /locus_tag="P5310_04955"
     CDS             <1399..>3006
                     /locus_tag="P5310_04955"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRU32742.1"
                     /translation="AQVIGPVNSYGDTEALRPHEGILRVGPDGFSIVPNIAKDWKFSQ
                     GGKVLTIYLREGMKWSDGAPFTADDIMFWYEDVLLNNELTPTKPAEWSPGGKLMKLDK
                     INNYTVRATFAVPYPVATIYLAHSAGVAGRFFLPKHYLMKFHPKYTPTDKIMEQAKKE
                     GFNSWYQYFGKYADYYGGGGMAIDAGAPTLQTYYIEKRELDHVILKRNPYYWKIDTAG
                     NQLPYIDEILVTDISNLEVVNTKIASGEVDIAEIGTTMENYPFYMENRERGNYRVLLW
                     QSIIGADVTYQLNLTYKEDPVLRNIFRDARFRRAMSLALNRDEINSILYLGLGTPRQA
                     TVIPQSPYFEESFAKAYIEYNPKEANRLLDEMGLKKGPDGYRLRPDGKRLEILLEYED
                     NLETPKGKTTEMAVHYWDAIGVKVTPKVISSSLKSQRTSANLIQFGLWHADRAGFLFT
                     TEPYYWVPIEVRSEALWCVEWGRWFASGGKAGEEPPAEIKKVRENWEKMKSAMDEKER
                     VRLGKEILKSNAENLWTIGTVGLAPHP"
BASE COUNT          930 a          516 c          610 g          850 t
ORIGIN      
        1 tttttgtctg ccttcatatc ctttaaatgg ataagattta tacggtctat atatttcttt
       61 aagaattccg cagggtcaag tccagcaaat tttatccaga aaacatccac ctcaagttta
      121 aggttttccg gatctgtatt ttctataagg atttctatac cgtatttatc tccaaacttt
      181 tcaaattcaa agttatgatt atgatacgaa agagtaagtc catcagccct taatctttca
      241 cctatagagt tcagctcttc agctgttttc ttccactctt ctatactgtt tcttctatct
      301 tcaggaagcc atggacaaac tatattctta ttccctaact ccagattaaa tttcactgta
      361 ttatcaaaat ctttctccag gagatcaatt gggatatgac tcccacatac ctttaaatcc
      421 agagaatcta aaatttgttt cagttcatca ctttttaccc cataagttcc agcaagttct
      481 atacccttat atcctatctc agctactttt tttaccgtct caataaaatt tttctctgcg
      541 taatctctca ctgtatacat ttgaagtgct atcggcttca ggttcatatt gaattacctc
      601 ctctttttat cgtacttatt acctctattt tataaaatat ttatggcaat ttcaacctac
      661 tgtaacctgc aaaccctgat aaatctcaag gtagctcaat aaatgcactt taaaaagttg
      721 aatgttcctt cttaccggga taaagacagt atcattaact caaaagtatt atagcatcta
      781 ttctggatgg tattcaaaat tatatatcag tttttctgga ctattgcatt tgaagacgtt
      841 ttcaaatata attattcaag agagaaggag gaggtgatat atggaatata taaacagaaa
      901 aatttttaag cagtttggtc caatctggtt accctaagaa ggggaaacaa ataattctaa
      961 gaaggaggat caaaaatgag aaggattttt actgtagtgt tagtctttgt attattaatc
     1021 agtgttgttc ctatctcttt ttctcaggaa gccaagtttc ataaacagta cagtactccc
     1081 acagattact ataaggctac cgggaaaagg ataaataagt acagcgaatc acctgtatta
     1141 gctgagatgg taaggcaggg aaaacttcca cctgtaaaag aaagacttcc aaaagaacca
     1201 atagtcatat ctccagtgga ggagattgga cagtatggtg gaaaggcaca ggttatagga
     1261 cctgtaaaca gttatggtga tacagaggct cttcgtccnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnag gcacaggtta taggacctgt aaacagttat ggtgatacag
     1441 aggctcttcg tccgcacgaa ggaatcctta gagttggtcc tgatggcttc tctattgtac
     1501 cgaatatcgc caaggattgg aaattctctc agggagggaa ggtccttacc atatacctcc
     1561 gtgaagggat gaagtggtca gatggagcac cttttaccgc tgatgatatt atgttctggt
     1621 atgaagatgt cctactaaac aatgaactta ccccgacgaa gcctgcagaa tggtctcctg
     1681 gcggaaagct tatgaagctg gataagataa ataattatac tgtcagggct acttttgcag
     1741 ttccatatcc agttgcaact atatatttag cccattctgc aggggttgcc ggaagattct
     1801 ttctaccgaa acactatctt atgaagttcc atcccaaata taccccaacg gataagatta
     1861 tggaacaggc taagaaggaa ggatttaata gctggtatca gtattttggt aaatatgcag
     1921 actattatgg tgggggaggg atggcgatag atgctggagc acctacactt caaacatact
     1981 atatagagaa gagagagtta gaccatgtca ttctaaagag aaatccctat tattggaaga
     2041 tagatacagc ggggaatcag ctcccttata ttgatgagat attggtaaca gatatatcaa
     2101 acttagaggt agttaatacc aaaatagcat ctggagaggt agatatagct gaaataggta
     2161 ctactatgga gaactatccc ttctatatgg aaaataggga gaggggtaat tatagggttt
     2221 tactttggca atcaataata ggagctgatg tcacctatca gttaaacctt acatataagg
     2281 aagatccggt attaaggaat atcttcaggg atgcaagatt cagaagagcg atgtctttag
     2341 ctctcaacag ggatgagata aatagcatct tatacctagg tcttgggacc ccaaggcagg
     2401 caactgttat acctcaatct ccttattttg aagagtcatt tgccaaggct tacatagagt
     2461 ataatccaaa agaggcaaat agattactgg atgagatggg gttaaagaag ggtccagatg
     2521 gataccgcct aagaccagac ggaaagagat tagagatact ccttgaatat gaagataatc
     2581 tagagactcc aaaagggaaa acgactgaaa tggcagtaca ctattgggat gccataggcg
     2641 taaaggtaac tccaaaggta ataagtagct ctttgaagag tcaaagaact tcagcgaatc
     2701 ttatacagtt tggattatgg catgcggata gggcaggttt cttgtttacc actgaaccct
     2761 attactgggt gcctatagag gtaaggagtg aagccctttg gtgtgtagaa tggggacgat
     2821 ggtttgctag tggaggaaag gcaggagaag agccccctgc agagataaag aaggtaaggg
     2881 aaaactggga gaagatgaaa tctgcgatgg atgagaagga aagggttaga ctcggtaagg
     2941 agatacttaa gtctaatgcg gagaatctat ggacgattgg aaccgtagga ttagctccac
     3001 atccaa
//
LOCUS       DAOTKU010000175         3002 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_26905_length_3002_cov_3.039362, whole genome shotgun sequence.
ACCESSION   DAOTKU010000175 DAOTKU010000000
VERSION     DAOTKU010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26905_length_3002_cov_3.039362"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(137..1048)
                     /locus_tag="P5310_04960"
     CDS             complement(137..1048)
                     /locus_tag="P5310_04960"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HRU32743.1"
                     /translation="MNSVMMYCDPDYKITRDPAVVKFMDKYYLYYTTTDGERHMIGIA
                     TSKDLINWTKAGEIHPTEEYEGKGICAPFAGIDRDGNLFILYQTNYFSLNEGIAFAFS
                     GDGINFTKPGDINPVYTVKNEWSLPRAIDADLIEVGGKRFLYFATRLKDKTSEQVIGL
                     ALSNDEKPFSFIDLDHPVLRGEYAWEKNFVEAPSSCYKRDKFIMFYGGGTNPQYVGVA
                     ISDDGVNWEKLSDEPFIKPDRIVKAGDLLWKQTDATHPFFFSDGDRDYIFFHSDYDHN
                     GEWPRASAITFCEIEWDSFYPRVIACR"
     gene            complement(1128..>3002)
                     /locus_tag="P5310_04965"
     CDS             complement(1128..>3002)
                     /locus_tag="P5310_04965"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRU32744.1"
                     /translation="PAFSQQKNRSGQYASIKEYQKAMGKKIARFNEAPQLAKLVEQGK
                     LPSVEKRLPQEPAVVEPVEEIGEYGGTWRRSLLGVSDSMGYRYLVYDPIVRWSREGLK
                     VIPNVAERWRVLDGGKTYVFYIRKGIKWSDGQPFTADDIMFWYNDILLNKDITPVFPA
                     WLRVGDTPCKVEKIDDYTVRFSFKDVYALFLENLAFQGEVFVPAHYLKQFHPKYTSAE
                     KLEAEAKRLGFQSWVQLFAQKNSYFLEPDRPVISAWKVTRAFPGDPMIAERNPYYWKV
                     DTMGNQLPYIDRILCSLSSNPEVVLMKALNGEIDMQFRHLGSSDFTLLLSNRDKGNYR
                     VLKAVFPGIAAATYFNMNCKDQVLRKIFEDRRFRIALSLAIDRNTINQLFYNGLLKPL
                     QATSVSYDPMWTKEIGESYIEYNPGQANKMLDSMGLTKRDKDGYRLRPDGKPLELTIE
                     VYDNTDVYDLVASFWSKVGIKTVVKSEERSLWTVRVTGGEHQVAGYAVEGLLWQIDPG
                     RYVPLSEFTYWAPLYGLWYATQGKSGEKPPQEILRLIELYEKFVSTPSEKARTLIGRD
                     ILRVHSRNLWMIGILGELPRPLVVNNNFRNVPADVFDDYRMQCEGKLNPEQFFIKSSK
                     "
BASE COUNT          898 a          701 c          491 g          912 t
ORIGIN      
        1 aaaatactgg ttcaaagaag agtaaaaagt caataaaatc tcagattcta aaggagaata
       61 gaggtgtggc atttaagaga ggcagatatc ccctttcatt tacctccacc tcacgctcct
      121 gttattttct aaaaagttat ctgcaggcaa taactctggg atagaatgaa tcccattcaa
      181 tctcacaaaa agtaatcgca gatgccctcg gccattctcc attatggtca taatcactgt
      241 ggaaaaatat gtaatctcta tcaccatcag agaaaaagaa ggggtgagtt gcatcggtct
      301 gcttccataa aaggtctcct gccttaacaa ttctatctgg ctttataaat ggttcatccg
      361 acaatttttc ccaatttact ccatcatcag aaatagccac tccaacatac tgtggattgg
      421 ttcctccacc ataaaacata ataaacttat cccttttata acaggagctt ggggcttcaa
      481 caaaattctt ctcccaggca tactctcccc ttaaaacagg atgatccaga tcaataaaac
      541 tgaacggctt ttcatcatta gataaagcca gaccgattac ctgctcgctg gttttatctt
      601 taagtctggt ggcaaagtat aaaaatcttt tacctccaac ctcaattaaa tctgcatcta
      661 tagccctggg taaagaccat tcatttttaa cagtataaac aggattaata tcccctggtt
      721 tagtaaaatt gatcccatct ccagagaagg caaaggctat gccttcattc agagaaaaat
      781 aattcgtctg ataaagtata aacaggttac catccctgtc tataccagca aatggtgcac
      841 agattccttt accctcatac tcctctgtgg ggtgaatctc ccctgctttt gtccaattta
      901 tcaggtcttt acttgttgct atacctatca tatgtctttc accatctgta gtggtatagt
      961 acaaataata cttatccata aactttacaa ctgcagggtc tctggtaatt ttgtaatcag
     1021 gatcacaata catcatcaca ctattcatct aattattcct ccaaaatgtt agactgtcag
     1081 gacaagactt ctaaaaccat accatcctga cagtctttaa agtgcagtta cttagaagat
     1141 tttataaaaa attgctctgg attcagtttt ccttcacatt gcattctata atcatcaaaa
     1201 acatcggcag ggacattacg gaaattatta tttacaacca atggtctggg aagttctccc
     1261 agtattccta tcatccaaag gtttctagaa tgaaccctta aaatatctct accaatcaat
     1321 gtcctcgcct tttcacttgg agtagataca aacttctcat ataactctat taacctgagt
     1381 atctcctgag gaggtttttc tccagatttt ccctgtgtag cataccagag cccatataga
     1441 ggtgcccaat aagtaaactc agacaggggg acatatcttc ctggatctat ctgccacagg
     1501 agcccttcca cagcatatcc agctacctga tgttctccac ctgttacacg gactgtccag
     1561 agacttctct cctcactctt gacaactgtt tttataccaa ccttactcca gaaagaagcc
     1621 accaggtcat aaacatcagt gttatcatat acctcgatgg tcagctctaa gggttttccg
     1681 tctggtctta atctataacc atccttatcc ctcttagtta atcccatcga atccagcatc
     1741 ttgttagctt gtccagggtt atactctata tatgattccc ctatttcttt agtccacata
     1801 gggtcgtaag atacgctggt agcttgaaga ggtttaagta aaccattata aaataactga
     1861 tttatggtat ttctatctat agctaaagat agggcaattc taaatcttct atcctcaaaa
     1921 atctttctca ggacctgatc cttacaattc atattgaaat atgtggctgc cgctattcct
     1981 ggaaagaccg ctttaagtac cctgtaatta cccttatctc tgttagaaag aaggagtgtg
     2041 aaatccgaag agcctaaatg tctgaactgc atatcgatct ctccatttag agccttcata
     2101 agaacaacct cgggattaga gctcagtgag cataatatac ggtcaatgta agggagctga
     2161 ttccccatgg tatccacctt ccagtaatac gggtttcgtt ctgcaatcat aggatcaccc
     2221 ggaaaggcac gggtaacctt ccatgctgaa ataactggac gatctggttc taagaaataa
     2281 ctatttttct gagcaaataa ctgtacccaa gattggaatc ccagtctctt tgcttcagct
     2341 tcaagctttt cagccgaggt atatttgggg tggaattgct tgagataatg ggcagggaca
     2401 aatacttctc cctgaaaggc aaggttctcc agaaatagtg catatacatc tttgaaagaa
     2461 aaacgaactg tatagtcatc aattttttct accttacaag gagtatctcc aactctgagc
     2521 caggcaggga aaacgggagt tatatcttta ttcagaagaa tatcgttata ccagaacata
     2581 atatcatcag cggtaaaggg ttgaccatca gaccatttga tacctttcct tatataaaat
     2641 acatatgtct taccaccatc taaaactctc catctctctg ccacattcgg tataaccttt
     2701 aatccctctc tggaccatct cactataggg tcatacacaa gatatctgta tcccatacta
     2761 tctgatacac ccagaagtga tcttctccaa gttccaccgt actcaccaat ctcttcaact
     2821 ggttcaacaa cagctggttc ctggggtaat ctcttctcca cagaaggtaa ctttccctgc
     2881 tctactaatt tagccagttg cggtgcttca ttaaacctgg caatcttttt acccatagct
     2941 ttctgatatt cctttataga agcatactgt cctgaccgat tcttctgctg agaaaaggcc
     3001 gg
//
LOCUS       DAOTKU010000176         2997 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_26957_length_2997_cov_2.996941, whole genome shotgun sequence.
ACCESSION   DAOTKU010000176 DAOTKU010000000
VERSION     DAOTKU010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2997
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26957_length_2997_cov_2.996941"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1723
                     /locus_tag="P5310_04970"
     CDS             <1..1723
                     /locus_tag="P5310_04970"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HRU32745.1"
                     /translation="DGLIPLAYLLNDESLIKNAEKWINWTLNSQTEEGNFGPPDNPDW
                     WTRMVMLKALIQYYEATLDPRIIEFMGRYFDYQFRNIEKRPLESWAMARGAENIYSIF
                     WLYNKTGESFLLDLADIIFGQTLDWVDLFTNFPYTRPIKFYYDFQKFREVFNKVQDII
                     NYHSHHVVNVAMGIKLPALFYLYTGEERYKKAVKSAIENLTRYHGVANGMFTGDEHLS
                     GRDPSQGTELCAVVEYMFSLQNIIRIFDNNIEYADIIEKVAYNALPATFTDNMCAHQY
                     DQQVNQVLVSVAKRNWYNNNDDSNIFGLEPNFGCCTANMHQGWAKFVKNLWFATEDNG
                     LTATVYAPSSVNIKINNTDIKVSEETDYPFNEQIKFTISCKEAVTFPLRLRIPGWCQD
                     GEIKLNGKKLSNVTPGSYYKITKEWQDGDMIELRLPMGIKINSWLNNSISVERGPIVF
                     ALKIREEWKKIAGQEPFADWEVYPESPWNYALVVDKESPEDYFSVEAGNVSNIPFNSD
                     KPPIILRCKAKRLEDWKLEDNSAGKLPVSPVYSDTELEDVTLIPYGVAKLRIAQFPYH
                     LPYIDTH"
     gene            1837..>2997
                     /locus_tag="P5310_04975"
     CDS             1837..>2997
                     /locus_tag="P5310_04975"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HRU32746.1"
                     /translation="MNRVLPVEEPITRSIAYIKESGRRLGLREEEMDAIIHPQETRIF
                     RLSCKILGKTVTFSGVLCLHNNARGPYKGGIRIAPDVDIWETVELGRLMTLKTAVSDI
                     EFGGGKTGIRVDWNYLYKLYDKKPQDREFEKIVSLDIVEYYAQIYRDLFSKHIYIPAP
                     DMGTGPDEMAFIYNETMDPASVTGKPEGTHGWLPGRKESTGYGCACVTRISVERIMGK
                     DISEVSVAIQGFGNVGSYVAKYLYESGAKIVGISDIFGGVYDEHGLDIEKLTEYSKRF
                     GTVVGYEGKSISNAELLCLPVDVLIPAAAGNVIGVEEAKKLQTKLIVEAANVPITLEG
                     MEILNEREIPVIPDIIANSGGVIASMEEYSHSLSALKTRREEVFKVLEEKLGQ"
BASE COUNT          976 a          503 c          702 g          816 t
ORIGIN      
        1 cgatggatta attccattag catatcttct taatgatgaa agcttaataa aaaatgcaga
       61 aaaatggata aactggacac tcaacagtca gactgaagag ggaaactttg gtccacccga
      121 caaccctgac tggtggacca ggatggtaat gctgaaagct ctaattcagt actatgaggc
      181 aactttagat cctcgaatca tagagtttat gggaaggtat tttgactatc agtttaggaa
      241 tatagaaaaa agacctcttg agagctgggc tatggcaaga ggagcagaaa atatatattc
      301 catcttctgg ttatacaata aaacaggaga atcattcttg cttgatctgg ctgatataat
      361 ctttggacag acactggatt gggtagatct gtttacaaat ttcccctata caagacccat
      421 caagttttac tacgattttc aaaaatttcg agaggttttt aataaggtcc aagatattat
      481 aaactaccat tcccatcatg ttgtaaatgt agctatgggg ataaaacttc ctgccttgtt
      541 ttatctctat accggagagg aaagatataa gaaggcggta aagtcagcga tagaaaatct
      601 tacccgatat catggtgttg ctaatggtat gtttaccggt gacgaacatc ttagtggaag
      661 agacccttct caagggacag aattatgtgc ggttgtagag tatatgttct ctcttcagaa
      721 tattataaga atatttgaca ataatataga gtatgcagat atcattgaga aagttgccta
      781 taatgcattg cctgcgactt ttaccgataa tatgtgtgca catcagtatg accagcaggt
      841 gaatcaggtc cttgtctcgg ttgcaaagag aaactggtat aataacaatg atgattcaaa
      901 tatattcggt ttagaaccaa actttggttg ttgcactgca aatatgcatc agggatgggc
      961 aaaatttgta aagaatctat ggtttgctac tgaagataat ggacttactg caactgtata
     1021 tgctccgtcc tctgttaata taaaaataaa caatacagac attaaagtaa gcgaagagac
     1081 agattatcca tttaatgagc agattaagtt cactataagc tgtaaggaag cggtcacttt
     1141 tccactgagg ctgaggatac cgggctggtg ccaggacgga gagataaaac ttaatggtaa
     1201 aaaactgtcc aatgttaccc ctgggagcta ttataagatt acaaaagaat ggcaggatgg
     1261 agatatgata gagctgagac tacctatggg aataaagatt aattcctggt taaataactc
     1321 tataagtgta gagagaggac ctatagtatt tgcattaaag atcagggaag agtggaagaa
     1381 gatagctggt caagagccat ttgcagactg ggaagtatat cctgagagtc catggaatta
     1441 tgcattggtg gtagataaag agtccccaga agactatttt tcagtagagg cgggtaatgt
     1501 ttccaatatt ccatttaact cagataaacc accaattatc ttaaggtgca aggctaaaag
     1561 gctggaagac tggaaattag aggataattc tgctggtaag ttacctgtga gtcctgtcta
     1621 ttcagataca gagttagaag atgtaactct aattccatac ggagttgcca agctcaggat
     1681 agctcagttt ccatatcatt taccctacat tgacactcat tgacatttta taaacttact
     1741 tatataattt ttggtaaagg cttcataata gatatataat aatataacaa atattaaaaa
     1801 ttaattttga acctttgaat tggaggtggt tgaaaaatga acagagttct tcctgtagag
     1861 gagcccataa ccaggtcgat cgcctatata aaggagagtg gcagacgtct tgggctcagg
     1921 gaagaggaga tggatgcgat aatccatcct caggagacca gaatcttcag gctttcatgc
     1981 aagatactgg gtaaaacggt aacattttca ggggtgctgt gccttcataa taatgcacgt
     2041 ggaccataca aaggaggtat ccgtatagct cctgatgtgg atatctggga gactgtagaa
     2101 cttggaaggc ttatgacact caaaaccgcc gtctcagaca tagaatttgg aggaggaaag
     2161 actggaatca gggtcgactg gaattatctc tacaaactct acgataagaa gccacaagat
     2221 agagaattcg agaagattgt ttctcttgat attgtagaat attatgccca aatctaccgt
     2281 gacctgttca gcaagcatat ctatatcccg gcgcctgata tggggacagg acctgatgag
     2341 atggcattca tatataatga aactatggac ccagcttcag taacagggaa gccagaggga
     2401 actcatggat ggctcccagg gaggaaagaa agcacaggat acggatgtgc ctgtgtaaca
     2461 aggatatccg ttgaacggat tatgggcaag gatatctctg aggtaagcgt ggcaatccag
     2521 ggctttggaa atgttggaag ttatgtagca aagtatctat acgaatcagg tgcaaagatc
     2581 gtaggaatta gcgatatatt tggtggagtt tatgatgagc atggtctcga tatagagaaa
     2641 ctaacagagt actcgaaaag attcgggaca gtcgttggat atgagggaaa atctatctct
     2701 aatgctgaac ttctgtgcct tcctgtagat gtattgatcc cggcagccgc tggaaatgtg
     2761 ataggggtgg aggaggctaa gaaacttcag actaagctca ttgtagaggc tgccaatgtc
     2821 ccgattacac ttgaaggtat ggagattctg aatgagagag aaattccagt catcccggat
     2881 attatagcca actcaggggg cgtaatagcc tctatggagg aatactccca ttctctaagc
     2941 gcccttaaga caaggcggga agaggtcttt aaagttcttg aagagaagct tggtcag
//
LOCUS       DAOTKU010000177         2997 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_26960_length_2997_cov_2.405167, whole genome shotgun sequence.
ACCESSION   DAOTKU010000177 DAOTKU010000000
VERSION     DAOTKU010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2997
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26960_length_2997_cov_2.405167"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            151..1071
                     /gene="argF"
                     /locus_tag="P5310_04980"
     CDS             151..1071
                     /gene="argF"
                     /locus_tag="P5310_04980"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HRU32747.1"
                     /translation="MLYRRDLLSIEDLTVGDIENIFNLTNYLKTEEGKNHKPLSGKTV
                     ALYFEKPSTRTRVSFEVGIFQLGGYPLYLDNKSLQISRGETLADTARVLSRYVNCIVA
                     RTLKDETVRELARYADIPVINALTDREHPCQVLGDIYTVKEKIGSYKGIKVSYVGDGN
                     NVANSLILISSKLGINFSIASPEGYEIKNEIKEKAIKYAKDSGAVIEFYNDPTLAVKD
                     ADVIYTDVWVSMGEESEEEKRNMIFQNYKIDLQLLKLAKSEVKIMHCLPAIRGKEIAD
                     EVMDGPSSVIFDQAENRLYVQKAILSLILG"
     gene            1074..2414
                     /gene="glmM"
                     /locus_tag="P5310_04985"
     CDS             1074..2414
                     /gene="glmM"
                     /locus_tag="P5310_04985"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006569551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HRU32748.1"
                     /translation="MKKLFGTDGVRGVANLDLTPELALKVAKAGGYYLSAGSDKPCFL
                     VGRDTRCSGDMIAGAVIAGLCSVGVDVIDIGILPTPGIAYLTRKLKVNAGIVISASHN
                     PIEDNGIKFFDSNGYKLSDEMEEDIEQLLDMDFPRPNGVKIGKVKVLPDAYELYVDYL
                     IDQVGVRLDGLRVVLDCAYGAAYKVAPLVYRSLGADVISINDIPLGEKINVNCGSTYP
                     GIIREEIRKYPGSIGISFDGDSDRVVLVDEEGNILDGDSILAIWSIELFKREKLRNNT
                     VVGTILSNMGLEIALLKYGIRLIRSDVGDRYVLEEMFKSNAVIGGEPSGHIIYLPGVT
                     TGDGIYTSLMVLKILKETGRTLSSFKDIMIRYPQISRNIKVKDKIGILEDMRMKNLRE
                     YLKVTLAGRGRFIIRPSGTEPYLRIMLEGEDEEYLDRLMEEITGKVREIDNAYL"
     gene            2401..>2997
                     /locus_tag="P5310_04990"
     CDS             2401..>2997
                     /locus_tag="P5310_04990"
                     /EC_number="2.1.3.15"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HRU32749.1"
                     /translation="MHISDWFSRKPKYAVLKPEKKEIPEGLWTRCPKCLEIIYNKELE
                     RNLKVCTKCGYHFKLNAWERIFQLVDNKEFYELDGYLESVDALPFDDGIYIDKLKEDR
                     EKTGLTEAIVTGKGKLEGFDILLGVFDFGFIGGSMASVVGEKVTRLFEYGVKEQLPVI
                     IVTATGGARMYEGMLSLMQMAKTNAALGRYRKTRGLFIS"
BASE COUNT          980 a          401 c          716 g          900 t
ORIGIN      
        1 gagggcttca gatacagata atattaagaa ggttccttta ctttctaata ttcctatact
       61 gggtcagctc ttccaatcca gagatacaaa acaggaggag agagagctta ttataatgat
      121 tacagtaaat atggtaaagg aggaagaata atgctatatc gtagagatct tctttctatt
      181 gaggatttaa ctgtaggaga tatagaaaat atatttaatc tcactaatta tttaaagacg
      241 gaagaaggta aaaatcataa acccttatct ggtaagactg ttgccctgta cttcgaaaag
      301 ccctctacca gaaccagagt ttcatttgaa gttggtattt tccaattggg aggatatcct
      361 ctttatttag ataataagag ccttcaaatt tcaagaggag agaccctggc tgatactgcc
      421 agagtccttt ctagatatgt taattgtata gtggccagaa ctttaaaaga tgagactgtt
      481 agagaattag ccagatatgc tgatatacct gttataaatg ctcttacaga tagagaacat
      541 ccttgtcagg ttctcgggga tatatatact gtaaaagaaa agataggctc ttataagggt
      601 ataaaggttt cctacgtggg tgatggtaat aatgtcgcta attctcttat attaatttct
      661 tccaagctgg gcataaactt ttctatcgca tcacctgagg gatacgagat aaagaatgag
      721 ataaaagaaa aagcgattaa gtatgccaaa gattcaggtg cagttattga attttataat
      781 gatccaactc ttgctgttaa agatgcagat gtgatatata cggatgtctg ggtaagtatg
      841 ggggaagaat cggaggagga aaagaggaat atgatcttcc agaattataa gatagatctg
      901 caattattga agttagctaa atctgaggta aaaataatgc actgtctacc agctataagg
      961 ggtaaagaga tagctgatga ggtgatggat gggccttctt cggtaatttt tgaccaggct
     1021 gaaaatagat tatatgtaca gaaggcaata ctttctttaa ttcttggatg aatatgaaga
     1081 agttatttgg gactgacgga gtaaggggag tagccaactt ggaccttact ccagagcttg
     1141 cattaaaagt ggctaaggct ggggggtatt atttaagtgc aggcagtgat aaaccctgtt
     1201 ttctggtagg cagggatacc agatgctctg gcgatatgat agcaggggcg gtgatagcag
     1261 gattatgttc agtgggggtt gatgttattg atattggtat attacctact cctgggatag
     1321 cctatcttac cagaaagtta aaggttaatg cagggattgt tatctctgct tctcataatc
     1381 ctatagaaga taatggaata aagttttttg actctaacgg ctacaaattg tctgatgaga
     1441 tggaagaaga tattgaacag cttttggata tggactttcc aagaccaaat ggagttaaga
     1501 tcggtaaagt taaggtctta cctgatgcgt acgaattata tgtagattat cttatagatc
     1561 aggttggggt acgtcttgat ggattgagag tagttttgga ttgtgcctat ggggctgctt
     1621 ataaggttgc tcctcttgtt tacagatctt taggcgcaga cgttataagt ataaacgata
     1681 tccccttggg tgagaaaata aacgttaact gtgggtcaac ttatcctggt attatcaggg
     1741 aagagatcag aaaatatcca ggaagtatag gcatctcatt tgatggagat tcagataggg
     1801 tggttcttgt cgatgaggaa ggaaatatac ttgatggaga cagtatcctg gctatatgga
     1861 gcatagaatt atttaaaagg gagaagctga gaaataatac agttgttgga acaattttaa
     1921 gcaatatggg gttagaaatt gccctattga aatatggtat aagacttata aggtctgatg
     1981 ttggggatag atatgtcctg gaagagatgt tcaaaagtaa tgctgtaata ggaggagaac
     2041 catctggaca tataatctat cttcctggtg ttactactgg tgatggaatt tatacatccc
     2101 ttatggtact taagatttta aaagaaactg gtaggactct ttcttcattt aaagatatta
     2161 tgattcgtta tcctcaaatt tccaggaaca taaaagtaaa ggataaaatt ggtattctag
     2221 aagatatgcg tatgaagaat ttaagagaat atttgaaggt tactctggca ggtaggggta
     2281 gatttattat cagaccttct ggtacagaac cctacttgag gataatgcta gaaggtgagg
     2341 atgaagaata tctggatcga ttaatggaag agattactgg taaagtgagg gagatagata
     2401 atgcatatct ctgattggtt ctccagaaaa cctaaatacg ctgttctaaa accagaaaag
     2461 aaagaaatac ctgaaggtct atggaccaga tgcccaaaat gtctggagat aatctataat
     2521 aaagaactgg aaagaaacct taaagtttgc actaaatgcg gatatcattt taaacttaat
     2581 gcctgggaaa ggatatttca gcttgttgat aataaagaat tttatgagtt ggatggatat
     2641 ttagaaagcg tggatgcatt gccatttgat gatgggattt atattgataa actcaaagaa
     2701 gatagagaga agacaggatt gacagaggct atagttaccg gcaagggtaa attggaagga
     2761 tttgatatcc tcctcggggt ctttgatttt ggcttcatag gtggaagtat ggcttctgta
     2821 gtaggtgaga aagttactcg actttttgaa tacggggtta aagaacagtt acctgttatt
     2881 atagttacag caacaggtgg agccaggatg tatgaaggga tgctctcttt aatgcagatg
     2941 gcaaagacaa atgctgcact gggaaggtat agaaaaacca ggggattgtt tatatct
//
LOCUS       DAOTKU010000178         2994 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_26992_length_2994_cov_3.293978, whole genome shotgun sequence.
ACCESSION   DAOTKU010000178 DAOTKU010000000
VERSION     DAOTKU010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26992_length_2994_cov_3.293978"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..831)
                     /locus_tag="P5310_04995"
     CDS             complement(<1..831)
                     /locus_tag="P5310_04995"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HRU32750.1"
                     /translation="MRETLYDMISANKIKTWIFMIVFSLLLGFIGYIIVNLFAWGTFG
                     YIGFAIFIILYNLITYYYSDRIALASVGAKPADPVQFRTLHNVVEEVALAAGIPKPKV
                     YVIDDPSPNAFATGRSPENASIAVTTGMLLLVDRSELQGVVAHEISHIRNYDMLIMTI
                     AAIMGGLIVLLRDFIFFFRPSRDNEKGSSGILYLIGILLAIISPILVALIRTAISRQR
                     EYLADASGAMIIRDPSALADALEKLLHFQKKMVNASAATAHLFIVNPFGEDRTSITGL
                     F"
     gene            complement(845..1381)
                     /locus_tag="P5310_05000"
     CDS             complement(845..1381)
                     /locus_tag="P5310_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003871013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HRU32751.1"
                     /translation="MVWLIIIALVLYVITIYNRMITLKHRVENAWAQIDTQLKRRYDL
                     IPNLVETVKGYAAHEKEIFEKVTELRYKAMSAGNISDASQANNELTQALRSLFAIAEA
                     YPELKANENFLSLQEELSNTENKIAFARQFYNDTVLEYNRALEMFPTNILARWMNLTP
                     RVYYQIPPEETGPVKVQF"
     gene            complement(1448..2755)
                     /gene="murF"
                     /locus_tag="P5310_05005"
     CDS             complement(1448..2755)
                     /gene="murF"
                     /locus_tag="P5310_05005"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HRU32752.1"
                     /translation="MKVKEIASLINGEVQGLPPETYIVSIGIDSRMIDRGGLFFALKG
                     ENNDGHNFIRQAINNGAIGAVMHNRLPYPGIIVKDTTDALFNLSSGIRERYPIPLIGV
                     AGSSGKTTSKELIYLVLGGKFRVIRSEGNQNTQFSLPIMVFKTPRCEIAVAELGIRKP
                     GDMNLLARITRPTTVVFTHLDREHMEFFGSFEDVIREELSIIEEIDGLKVIYNRDDEN
                     LKNLNGTSYGIESDSDIRGFSIKVHSEGTSFKVMYPDGEVFEVNIRAFGRHMVEDALA
                     ALAAGWVYGIPSELAIKALESFMPIWGRMEPVKLSNGTLVIFDGYNANPLSMKRALET
                     VSSLDFNRKLYILADMLELGSETEAAHRDLGYMLKYADGDIILIGESMAITHQILGER
                     SIHFKDLALALSYIKENIYKYDMVLIKGSRGMKLERIMEAVQK"
     gene            complement(2752..>2994)
                     /locus_tag="P5310_05010"
     CDS             complement(2752..>2994)
                     /locus_tag="P5310_05010"
                     /inference="COORDINATES: protein motif:HMM:NF013962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HRU32753.1"
                     /translation="KLLGFRKGARFTLHIENRVGAVADLANIFKDLGINIISIVAFEE
                     EREEEGNVVVRVDTKDTEKVVSALKSAGIKVIHFSR"
BASE COUNT          822 a          703 c          533 g          936 t
ORIGIN      
        1 gaaaagacca gttatagaag ttctatcttc tccaaatgga tttactataa atagatgagc
       61 ggtggctgca ctagcattta ccatcttctt ctggaagtgg agtaacttct ccagagcatc
      121 ggcaagcgct gatgggtctc tgataatcat agccccggat gcatcggcga gatactcccg
      181 ctgtcttgag attgctgtcc ttatcagtgc aaccagtatc ggagatatta tggcaagaag
      241 tatcccgata agatacagta taccactgct acccttttca ttgtctctgg acggtctaaa
      301 gaagaagata aagtccctga ggagcactat gagtccaccc attatagcgg cgatcgtcat
      361 aatgagcata tcgtagttcc ttatatgtga tatctcatga gcaacgacac cctggagttc
      421 actcctatcc accaggagga gcataccagt agtaactgct atagatgcat tctctggact
      481 tcttccggtg gcaaatgcat ttggagatgg gtcatctatc acatagacct tgggttttgg
      541 aatccctgct gctagagcaa cctcttccac cacattatga agggttctaa actgaacagg
      601 gtcggcaggc tttgccccga cggatgctag tgctatcctg tcactgtagt aatacgttat
      661 caggttatag aggatgatga atatagcaaa tcctatataa ccaaacgtcc cccacgcaaa
      721 gagattcact ataatatatc caataaatcc taaaaggaga ctgaatacta tcataaagat
      781 ccatgtcttt attttattag ccgatatcat atcatacaat gtttctctca cagagaatca
      841 cctcctaaaa ctgtaccttt actggtcctg tctcctctgg aggaatctga taataaactc
      901 tgggggtaag gttcatccac ctggccagta tatttgtggg gaacatctcc agagccctgt
      961 tgtactccaa tacggtatca ttatagaact gacgggcaaa tgcgatctta ttttcggtat
     1021 ttgatagttc ttcctgaaga ctcaagaagt tttcatttgc cttaagctca ggataggcct
     1081 cagcaattgc aaagagtgac cttagagcct gggtgagctc gttatttgcc tgagatgcat
     1141 cggatatatt tccagcactc atcgccttat atctgagttc tgttaccttt tcaaagatct
     1201 ccttttcatg cgcggcgtac cccttaaccg tctctaccag attcggtatc aggtcatatc
     1261 tcctctttaa ctgtgtatcg atctgggccc atgcattttc aactctgtgc tttagagtaa
     1321 tcattcgatt atatatggta ataacataga ggaccagagc gatgataatt aaccatacaa
     1381 tagccatatg ttacatcacc ttccttctat attatagtat aacactaatt aataaaaact
     1441 gtatctctta cttctgtaca gcctccatta tcctttccag tttcatgccc ctggagccct
     1501 ttataagtac catgtcatat ttatatatat tctcttttat atatgaaagt gcgagggcta
     1561 aatctttaaa gtggatagat ctttcaccca gaatctgatg tgtaatagcc atggactctc
     1621 ctatgagaat tatatccccg tctgcatatt tcaacatata tccaaggtct ctgtgtgccg
     1681 cttctgtctc acttcccagc tcaagcatat cagcaagtat gtaaagtttt ctattaaaat
     1741 ctaaagaaga aactgtttcc agggctcttt tcatagagag aggattggca ttgtacccat
     1801 caaagataac cagtgtaccg ttactcaact ttactggttc cattctaccc catataggca
     1861 taaaactttc cagggctttt attgcaagtt cactaggtat accatagacc catccagcgg
     1921 cgagagctgc gagtgcatct tcgaccatat gacgtccaaa tgccctgatg tttacttcaa
     1981 aaacttctcc gtcaggatac atcaccttga agctggttcc ctcactgtgc acctttatag
     2041 aaaatcctct gatatctgaa tctgattcaa taccataact tgtgccatta agattcttca
     2101 ggttttcgtc atctctatta tagataactt ttaatccatc tatctcttct ataatactga
     2161 gctcttctct gattacatcc tcaaatgaac cgaagaattc catatgttct ctatcaagat
     2221 gggtaaaaac taccgtggtg ggtctggtga tcctggcaag taaattcata tcacctggtt
     2281 ttcttatacc aagttcagct acagcgattt cacatctggg ggttttaaag accatgatgg
     2341 gtagactgaa ctgtgtattc tgattcccct ccgatcttat cacccggaat tttcccccaa
     2401 gaacaaggta tattaactct ttagatgtgg ttttcccact acttccagcc actcctatga
     2461 gaggtatagg atacctctct cttattccgc tggacaggtt gaacagtgca tctgtagtat
     2521 ccttaacgat gataccgggg tatggaagcc tgttatgcat taccgcccct atagctccat
     2581 tatttatagc ctgccgaata aaattatgac catcattatt ctctccctta agggcaaaaa
     2641 agagaccacc tctatctatc atcctggagt ctattccaat agaaactata taggtctcag
     2701 gaggtaaccc ctgtacctcg ccattgatca ggctagctat ctcttttact ttcatcttga
     2761 gaaatgaatc acctttatcc ctgcagactt taaagcagaa actacttttt ctgtatcttt
     2821 tgtatcaacc ctgacaacga catttccctc ttcttctctc tcttcttcaa aggcaacaat
     2881 acttataatg tttataccaa ggtccttaaa tatattggca aggtctgcta cagctcccac
     2941 cctgttttct atatgaagtg taaatctggc tccctttctg aatcctaaca gctt
//
LOCUS       DAOTKU010000179         2993 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_27004_length_2993_cov_3.166440, whole genome shotgun sequence.
ACCESSION   DAOTKU010000179 DAOTKU010000000
VERSION     DAOTKU010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27004_length_2993_cov_3.166440"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            102..1328
                     /locus_tag="P5310_05015"
     CDS             102..1328
                     /locus_tag="P5310_05015"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRU32754.1"
                     /translation="MKKLRGRSLPQVRRFNRSLVLQTIRAHSPISRIELTELTTLTPT
                     TITSLVDEFIKYGLVKEIGNIKGGVGRSRTLISINSEAYYVLGIDLARISISAGIVDL
                     AGNLLTVKRVSSDLNQHFPVTLNTLEETIHSLLDEISPQIKEKIIAIGIGSPGPLSPS
                     KGVIISPPNFTGWSNIPLKEIMEREFGLPTFIENDAKACALGERWFGCCKNTDNFVYL
                     AVGTGVGAGIIIDGDIYRGEGELAGEIGHTTIDINGPRCSCGNYGCLEIYTSVISLID
                     RIEKEEGLKSLIRDGHIETLSSFYRSAREGNIRAVEILNDYCFWVGVGVVNIINTLSP
                     SAVVLGREALINGSDLIIPRVEKVVTERSFFTTSQQTRILASSIGQDTGVIGAATIAL
                     QEFYKSPYEMIKNKNK"
     gene            complement(1330..2127)
                     /locus_tag="P5310_05020"
     CDS             complement(1330..2127)
                     /locus_tag="P5310_05020"
                     /inference="COORDINATES: protein motif:HMM:NF013355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HRU32755.1"
                     /translation="MSWNLCGIELGSPLVAASGPLGWGKEFFEIFSTDGLGLFIPKTV
                     TLKPKVGNPPPRIVETTGGLINSIGLENDGVYEWLKLEDLYRSLDIPVMASIAGGSLE
                     ELSTLGEIMEKLDYIAGVELNLSCPNVEGENWSNNTTSVLEATRAVRKTYSRLLFVKL
                     PPKDNIFPYIEASRSGGADGFTLFNTLPACDRIKGGLSGPAIKPVYIRLLKEIMEEID
                     IPVMASGGIMTEKDVRDYLYLGVKAVQIGTAFFKDPDIAKRIYQELQ"
     gene            complement(2124..2846)
                     /locus_tag="P5310_05025"
     CDS             complement(2124..2846)
                     /locus_tag="P5310_05025"
                     /inference="COORDINATES: protein motif:HMM:NF021903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HRU32756.1"
                     /translation="MPEIYSGEVLENRSAGELFLLTVSIGKSIDIIPGQFFLIRIDRE
                     DTVLRRAFSIFDKNDDRLSFLYRVVGKGTGWLSERKTGYELNIFGPLGNGFSLVEGRS
                     LLVGGGTGMAPLYYLGKNINGDVDFLLGAQRKTFWSEELLSRYRDIGNLELVTEDGSS
                     GKKGLVTQYIKNTCYTKVYACGPMEMLKSINIDIPAEVSLESVIACGFGACRGCVVET
                     VSGEYKRVCKDGPIFDLREISI"
BASE COUNT          912 a          675 c          589 g          817 t
ORIGIN      
        1 gaaagagatt ggaaggcaat cttttcgccc tgtgagataa agagtttctt aatacctaag
       61 gataaaaatc tccctgtaaa agaggtgaac atcttagaag agtgaagaaa ttgagaggaa
      121 ggagtctacc acaggtaaga agatttaata ggagtttagt actccaaact ataagagctc
      181 atagccctat atctaggatc gagcttacag aactcacaac cctgacaccc accaccatta
      241 ccagcctggt agatgaattc attaaatacg ggttggtaaa agagataggt aatataaaag
      301 gaggggtagg tcgtagcagg acattgatca gtataaatag tgaggcatac tacgtcctgg
      361 ggatagatct ggcaaggata tccatctctg ccggtatagt tgacctggct ggtaatctgt
      421 taactgtaaa gagggtaagt tctgacctga atcagcattt tccggtaacc ctgaataccc
      481 tggaagaaac gattcattct cttctggatg agatcagccc tcaaataaaa gaaaagatta
      541 tagctatagg tatcggctct cctggacctt taagtccaag taaaggggtg ataatctctc
      601 cacctaactt tacaggatgg agcaatatcc ccctgaaaga gataatggaa agagaattcg
      661 ggcttcccac cttcatagag aatgatgcaa aagcctgtgc cctgggagag agatggttcg
      721 gatgctgtaa gaatacagat aactttgtct acctggctgt tgggactgga gttggagcgg
      781 gtattattat agatggagat atatacaggg gagagggaga gctggcagga gagataggac
      841 ataccacaat agatataaat ggtccaaggt gcagttgtgg taactatgga tgtctcgaga
      901 tatacacatc tgtaatctct cttatagaca ggattgaaaa ggaggaaggg ctaaagtctc
      961 tcatcagaga tggacatata gagacattgt cttctttcta caggtcagcc agagagggga
     1021 atataagggc tgtggaaatt ctaaatgact actgcttctg ggttggtgtt ggggtagtca
     1081 atataataaa tacactcagt ccatctgctg ttgtactagg aagagaggcg ctaataaacg
     1141 gttcagacct tatcatccct agagtagaaa aggtggttac ggagaggagc tttttcacaa
     1201 catcccagca gaccagaata ctggcatcaa gtatcggaca agatacaggg gtcataggag
     1261 cagcaacaat tgcactgcaa gaattctata agtcgccata cgaaatgata aaaaataaga
     1321 acaaataatc tactgaagct cctggtatat tcttttagct atatccgggt ctttaaaaaa
     1381 agcggttcct atctgaaccg cttttacccc cagataaaga tagtccctga catctttctc
     1441 tgtcattatc ccgccagaag ccataactgg aatatctatc tcttccatta tttctttaag
     1501 cagtcttata taaacaggtt ttatagctgg tccagagaga cctcccttga ttctatcaca
     1561 ggcaggaagt gtattgaaaa gtgtaaagcc atctgcccct ccagatctgc ttgcttcaat
     1621 atagggaaag atgttatcct ttggaggaag cttcacaaag agtagcctgg aataagtctt
     1681 tcttaccgct ctggtagcct ccaaaacact ggtagtatta ttactccagt tttctccctc
     1741 cacattggga caggagagat tcagttctac tccggctata tagtccagtt tctccataat
     1801 ctcccccaga gtggataatt cttctaaact accacccgct atacttgcca ttaccgggat
     1861 atcgagagac ctgtacagat cttccagctt taaccattca taaacaccgt cattttcaag
     1921 acctatagaa tttatcagac ctcctgtagt ctcaactatc ctggggggag gattaccgac
     1981 ttttggttta agggttactg tcttggggat aaataatccc agaccatctg ttgaaaagat
     2041 ctcaaaaaat tcctttcccc atccaagagg acctgatgca gctaccagag gactcccaag
     2101 ttctatacca cataaattcc agctcatatc gatatctccc tcagatcaaa tataggacca
     2161 tctttacata ctctcttata ctcacctgag acggtctcca caacacatcc cctgcaggca
     2221 ccaaaaccac aggctataac agactccaga gatacctcgg cagggatatc tatatttata
     2281 ctcttcaaca tctccatggg accacaggcg tatacttttg tataacaggt attctttata
     2341 tactgcgtta ccagcccttt ttttcctgaa gacccatctt cggtgacaag ttccagattg
     2401 cctatatctc tgtaccttga aagcaactct tcgctccaga aggtttttct ctgagcaccc
     2461 aataaaaagt cgacatcacc attgatattt ttccccagat aatagagagg agccatacca
     2521 gttcctcccc ctacaagaag tgatctcccc tcaaccagag aaaagccatt tcccagcggt
     2581 ccaaagatat tcagctcata ccctgttttc ctctctgaca accatcctgt tccctttccc
     2641 acaactctgt acaaaaagct caatctatca tcatttttgt caaagatact gaaggctctc
     2701 ctgaggactg tatcctctct atctatcctt ataagaaaaa actgacctgg aattatatcg
     2761 atggacttac caatagatac tgtcagcaag aataattccc ctgctgacct gttttccagg
     2821 acctctccac tatatatctc tggcatacac tatctttcca ttgaccattg ttacagcagg
     2881 gaaaccgtaa agttcaaatc catcgtaggg agaattcttc cctttcgata aaaatgacga
     2941 tacatctact ctttctctat aatcaggctt aaaaattacc aagtccgcct tct
//
LOCUS       DAOTKU010000180         2992 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_27015_length_2992_cov_3.877766, whole genome shotgun sequence.
ACCESSION   DAOTKU010000180 DAOTKU010000000
VERSION     DAOTKU010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27015_length_2992_cov_3.877766"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1261
                     /locus_tag="P5310_05030"
     CDS             <1..1261
                     /locus_tag="P5310_05030"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HRU32757.1"
                     /translation="SYQNLRFGVMLFISIFIAILILLILLLRKLLSYLKILRDISIEY
                     AKGNYSKDIHTSPFREINDIIDALQVMGREIREREEKIKEEQSYLSTLLLEMGEGVFI
                     LNQDKKFEFVNERLTEMLGYTKDEITRFNPIEIFAPGYRERISKIYSEGERHRERAEL
                     VSKDGRSIPVLCSVSPINLNSSITGYLSVVTDLTEIEEREKELQSALEEIKALNEELT
                     TRSQQLEIALARLDMKLFEVERAKENAEKLAIIDPLTGLFNRRYLEEKFSNELIKAKA
                     YNSYVSVVMADIDHFKRINDTYGHKVGDEVLKNLALIFRANIREDDTVARYGGEEFVI
                     ILHNVDKYDAYEIAERIRGEIEETSFEEIGIPEKVTVSFGISSFPEDGEELTDLLIKA
                     DQALYQAKSLGRNRVEVYTKPSESIHF"
     gene            1326..1613
                     /locus_tag="P5310_05035"
     CDS             1326..1613
                     /locus_tag="P5310_05035"
                     /inference="COORDINATES: protein motif:HMM:NF015602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HRU32758.1"
                     /translation="MENKPIDSISENIKALVLNPRPKIKPSETDMNEVKGFEFIDMHF
                     RFKLHKPTNEVIVQIVDSQTGEVLKEFPPEKILNMVSEFLKMSGFIIDKKV"
     gene            1623..>2992
                     /gene="fliD"
                     /locus_tag="P5310_05040"
     CDS             1623..>2992
                     /gene="fliD"
                     /locus_tag="P5310_05040"
                     /inference="COORDINATES: protein motif:HMM:NF018852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HRU32759.1"
                     /translation="MADSLFNIWGLASGIDTNSIIESMVTVAKTPVIRYQNQQSEIQW
                     KQEIWSTINTRLQNLQSAVSILTDKNSIVGKRGISSNDNVLSVSTTGAAPLGTYTFEV
                     VDIAMAHTVTSNVYASRSEKISFSDGLITINGKTISVGTQASLLDIAQAINQDVDIGQ
                     KVYASVVQVSSSDFRLVITSKNTGTTNSITFGGESNDVLISLGIVGEAKSNTYASYTE
                     PISFETGEITINGETINIGTSASLQDIVDAINNDLEISTKVKARITQEESSYRVSLIS
                     LTGSIETLDFSGGNDVLKTIGVLDSSGTLVNPNNTGGATTQLAKDLELTVSGISGTII
                     RQSNTVTDLFEGVTLNIKNTGTSTVTVVYDTSQAISNIENFVNVYNDTLSYIRTKLTE
                     EREEGIDFLSEADKEKMTSFEIEQHNEKLKVGLLRNDSTLIEIEDQLRSIVTGMVKND
                     TNTPLTS"
BASE COUNT         1046 a          491 c          682 g          773 t
ORIGIN      
        1 ttcttaccag aatttaagat tcggggttat gctatttata tcaatcttta tcgcgatact
       61 gattcttctg attctccttt taaggaaatt gctatcctat cttaaaatcc tcagggacat
      121 atcaatagaa tatgcaaagg gaaactactc taaggatatc cacacctctc ccttcagaga
      181 gataaacgac ataatagatg ccctccaggt aatgggaaga gagataaggg aacgggaaga
      241 aaagataaag gaagagcaga gctatctttc aaccctttta ctggaaatgg gagagggggt
      301 atttatatta aatcaggata aaaagtttga atttgtaaat gaaagactga cggagatgct
      361 aggctacaca aaagatgaga taactagatt taatcctata gagatctttg ctcctggtta
      421 tagagaaagg atatcaaaaa tttactctga aggggagaga cacagggaga gggcagaact
      481 ggtctctaaa gatgggagga gtatcccggt attatgttct gtaagcccaa taaacttaaa
      541 tagcagtata acaggttatc tatcggtagt taccgatcta accgagatag aagagagaga
      601 aaaagaactt caaagcgcac tggaagagat aaaagctctc aatgaagagt taaccacccg
      661 gagtcaacag ctggagatag ccctggcaag actcgatatg aaactgttcg aggtagaaag
      721 ggcaaaggag aatgcagaaa aactggctat aatagacccg ttaactggtc tgtttaacag
      781 aagatacctg gaagagaagt tcagtaatga gttgataaag gcaaaggctt ataatagcta
      841 tgtatctgtg gtaatggctg acatagacca ttttaaaagg ataaacgata cctacgggca
      901 taaggtgggg gatgaagtct taaaaaatct ggcgcttata ttcagggcta atatacggga
      961 ggatgacaca gttgccagat atgggggaga agagtttgta ataatattac acaacgtgga
     1021 taaatatgac gcatatgaaa ttgccgagag gataagaggg gagatagaag agacatcatt
     1081 tgaagagata ggcattccgg agaaagtcac cgtaagtttt ggtatatcaa gtttccctga
     1141 ggatggagaa gaacttacag acctcctgat aaaggcagat caggcattat atcaggcaaa
     1201 atcacttgga agaaacaggg ttgaagtata taccaaacca tctgagtcca tacatttcta
     1261 atattaaagt tttttaattt aaatccgatt ataaagttat tgaataaaga ttgtgggggt
     1321 ttattatgga aaataaacca atagatagta taagcgagaa tataaaagcg ctagttttaa
     1381 atcccagacc gaagataaag ccatcagaga cagatatgaa tgaagtgaaa ggctttgaat
     1441 ttatagatat gcacttcagg tttaagcttc ataagcctac caacgaggta attgtccaga
     1501 ttgtagactc tcagacagga gaggtattaa aagagtttcc accagagaaa atactgaata
     1561 tggtatcaga gtttttaaag atgtccggtt ttattataga caaaaaagta tagggggtat
     1621 ttatggctga ctcattattt aatatatggg gtttagcttc agggatagat accaatagca
     1681 ttatagagtc tatggttaca gttgcaaaga ctccggttat cagatatcaa aatcaacagt
     1741 cagagataca gtggaaacag gagatatgga gtacaataaa tacaaggctt cagaacctcc
     1801 aatcggctgt atctatcctt accgacaaga atagtattgt cggcaaaaga ggcatctcct
     1861 ctaatgataa tgtattatca gtctctacta caggcgctgc tcctctaggg acatatacat
     1921 ttgaagttgt tgacatagcc atggctcata cagttaccag caatgtctat gcaagcaggt
     1981 ctgagaagat ttcattctca gatggactta taacaataaa tggtaagaca atctctgttg
     2041 gtacccaggc ttcactttta gatatagctc aggctataaa tcaggatgtg gatataggac
     2101 aaaaggtgta tgccagtgta gtacaggtat cttccagtga cttcagactg gtcataacat
     2161 caaagaatac cggcactaca aacagtataa catttggggg tgaaagtaac gatgttctta
     2221 tctctctggg gattgtgggt gaggctaaga gtaataccta cgcatcttat accgagccca
     2281 tctcatttga gaccggcgag ataacgataa atggagagac cataaacata ggaacatctg
     2341 catccctgca ggacatagta gatgcaataa ataatgatct ggagataagt actaaggtaa
     2401 aagcaaggat aactcaggag gagtcttcat acagggtctc tcttatatct cttacaggct
     2461 ccatagagac gcttgacttc tctgggggta atgatgtatt gaagacaata ggcgttcttg
     2521 actcatccgg gacattggtc aatccgaata atactggtgg ggcaacaaca cagctggcaa
     2581 aggatttaga gcttacagtc tccgggatat ccggtacaat tataaggcag agtaatacag
     2641 tgacagacct gtttgaaggg gtaactttaa acataaaaaa taccggaaca tctacagtta
     2701 cagtggtcta tgatacatct caagctataa gcaatataga gaacttcgtg aatgtgtata
     2761 atgataccct ttcctatatc aggacaaagc ttaccgaaga gagggaagaa ggtatagatt
     2821 ttctctccga ggcggacaag gagaagatga caagctttga aatcgaacag cacaatgaaa
     2881 aattaaaggt gggtcttttg aggaatgact cgacattgat agagatagag gaccagttaa
     2941 ggtccattgt taccggcatg gtaaagaatg atacaaatac tcctcttacc tc
//
LOCUS       DAOTKU010000181         2988 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_27054_length_2988_cov_2.895329, whole genome shotgun sequence.
ACCESSION   DAOTKU010000181 DAOTKU010000000
VERSION     DAOTKU010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27054_length_2988_cov_2.895329"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="P5310_05045"
     CDS             <1..277
                     /locus_tag="P5310_05045"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRU32760.1"
                     /translation="RGSLIDEASLIERLARGDIFACLDVTDPEPPDINSPLRRLPNVI
                     LTPHIAGGNSVTDRWRQGRYIIDQIYKFITQGTMDYEVTKDMLTRIA"
     gene            287..1027
                     /locus_tag="P5310_05050"
     CDS             287..1027
                     /locus_tag="P5310_05050"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HRU32761.1"
                     /translation="MREKTLKLNLRSRVRVAEDIWEERIDAKIFSIDKTAILLCDIWD
                     NHWCSGAKKRVDILAPRINRVVSVARENGIQIIHSPSDTMDFYKGTVYRKRILEVPRI
                     EPVELLSFYEPPLPIDDSDGGCDSGEKPWYKAWTREHPAIEIRGNDVISDNGIEIYSF
                     LRYLNIDTLIYIGFHTNMCILKRPFGIRNMKRYGINCILVRDLTDAMYNPDMPPYVSH
                     EKGTELVIQHIEKYLCPTILSEDLEKLY"
     gene            1039..1833
                     /locus_tag="P5310_05055"
     CDS             1039..1833
                     /locus_tag="P5310_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HRU32762.1"
                     /translation="MEKKYVFSVFTKPWQNMPVKELGEFVSKLGFDAIEFPVRPGYQV
                     EPENVKEGLPKLARELKSFGIDIASIAGPTDEDTFIACATAGIPVIRVMCRVEEDGYL
                     ASEKKARKDLESLLPLCEKYNVKIGVQNHYGKFVSPNSAGLMRLVEKFDPNYIGIIWD
                     AAHNALGGEEPEMGLDIVWSHLCMVNLKNAFWRRKTGPELEAEWEIYWTSGKQGLASW
                     RRVAEYLKKRNYRGVICLTAEYTTKDEVDRLIAEDIKFARSLFYED"
     gene            1835..2803
                     /locus_tag="P5310_05060"
     CDS             1835..2803
                     /locus_tag="P5310_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018189321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRU32763.1"
                     /translation="MEDSKVRIAFIGCGVMANKVHYPSLTSFSDVEIVAICDVDTERL
                     NTTGDKYNIEKRYADYKKMIEETSPDAVYAIGQPHIMYDIWMWCLKEGLNLYIEKPMG
                     ITIHQARMLAYMAEKKGCITQVSFQRRAVPLLVRLKEECLKHGPIVHAVCEFYKYNIE
                     PYLEARDHMMDDGVHAIDTLRWICAGEVKRILSVTRNVLVPDINLISAILEFDNGSIG
                     VMINSWTSGRRIFRVQMHAPGIWVEADPEDKGRSYINGDTNGIEYDTKEVAGSSEFYV
                     YAGFQAKNREFIDCLKLKKQPGSNFSDALKTMEVAEKILAKALLED"
     gene            2806..>2988
                     /locus_tag="P5310_05065"
     CDS             2806..>2988
                     /locus_tag="P5310_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32764.1"
                     /translation="MEYVKPVKIELYFIPVKTRIPLKFGPEILTSVNCARVHMFVSDN
                     KGKVMDGWGETPLSVQW"
BASE COUNT         1026 a          450 c          708 g          804 t
ORIGIN      
        1 aagaggttca ttgatcgatg aagcctctct catagagagg cttgctaggg gagatatctt
       61 tgcctgtcta gatgttacag accctgaacc tccagatatc aatagtccat tgaggagatt
      121 acccaatgtc attcttactc cacatatagc aggaggaaat tcagtgacag atagatggag
      181 acagggtaga tatattatag atcagatata taaatttatt acccagggca ctatggatta
      241 tgaggttacg aaagatatgc ttacgagaat agcttaggac tttaagatga gagaaaagac
      301 tcttaagctt aacctcagaa gtagagtaag agtagccgaa gatatctggg aagagaggat
      361 agatgcgaag atttttagca tagataaaac agctatactt ctctgtgata tatgggataa
      421 tcactggtgc agtggagcta aaaaaagagt agacatactt gcccccagga ttaatagagt
      481 agtcagcgta gcaagagaaa atgggattca gatcatacat tctccatcag atactatgga
      541 tttttataag ggtactgtgt atagaaagag aatcttagag gtacccagaa tagaaccagt
      601 ggagttattg tctttttatg agcctcctct tcccatagat gactcagatg ggggatgtga
      661 ttctggagaa aaaccctggt ataaagcctg gacaagagaa catccagcga tagagataag
      721 aggaaacgat gtgatatctg ataatggtat agaaatatac agctttctaa gatacttaaa
      781 catagatacc cttatctata taggttttca cacaaatatg tgtatactca agagaccttt
      841 cggaataaga aacatgaaaa gatacggtat aaactgcatt ttggttagag accttacaga
      901 tgctatgtac aatcccgata tgcctccata cgtatctcat gaaaaaggta ctgagttagt
      961 aattcagcat atagaaaaat atctctgccc cactatattg agcgaagact tggagaaatt
     1021 atattaggag ggataaaaat ggaaaagaaa tatgtctttt cggtatttac aaaaccatgg
     1081 cagaatatgc cggtgaagga attgggggag tttgtaagta aactgggttt tgatgccatc
     1141 gagtttcctg taagaccagg ttatcaggta gaacctgaga atgtaaaaga aggattaccg
     1201 aaacttgcca gagagttaaa gagttttgga atagatatag ccagtatagc aggacctaca
     1261 gatgaggata cctttatagc ctgtgcaaca gccgggatac ctgtaatcag ggttatgtgt
     1321 agagtagaag aagatggcta tcttgcatct gaaaagaagg caagaaagga ccttgaatcg
     1381 cttctacctt tatgtgaaaa gtacaatgta aagataggag ttcagaatca ctatggtaag
     1441 tttgtgtctc caaattcagc tggacttatg agacttgtag agaaatttga tccaaattat
     1501 ataggaatta tctgggatgc agctcacaat gcactgggtg gagaagagcc agagatggga
     1561 cttgatatag tatggtcgca tctctgtatg gttaatctaa aaaatgcttt ttggagaaga
     1621 aagacaggac ctgaattaga agcagagtgg gaaatatact ggaccagcgg gaagcaaggt
     1681 cttgccagct ggagaagggt tgctgaatat cttaagaaga ggaactacag aggagttata
     1741 tgtttaactg cagagtatac aactaaagac gaggtggata gactgatagc tgaagatata
     1801 aaatttgccc gatcactatt ttatgaggat taagatggaa gactcaaagg ttagaatcgc
     1861 ctttataggt tgtggagtaa tggctaataa agttcattat ccatctctaa catctttttc
     1921 tgatgtggag attgttgcca tatgtgatgt agatacagaa agacttaata ctactggcga
     1981 caaatataat atcgagaagc gatacgcaga ttataaaaag atgatagaag aaacttcccc
     2041 cgatgctgta tacgccatag gtcaaccaca tataatgtac gatatctgga tgtggtgtct
     2101 aaaggaggga cttaatttat acatagaaaa acctatgggt ataactatac atcaggcaag
     2161 aatgcttgcc tatatggcag agaagaaagg atgtattact caggtaagct tccagagaag
     2221 agctgttcct ctacttgtaa gacttaaaga ggagtgtcta aaacatggac ctatagttca
     2281 tgcggtctgt gaattttata aatataacat agaaccatat ttagaggcta gagaccatat
     2341 gatggatgac ggtgtgcatg caatagatac actcagatgg atatgcgcag gagaagtaaa
     2401 gaggatactc agtgtaacca ggaacgtttt agtccctgat atcaacctta tatcagcaat
     2461 acttgaattt gataatggtt ctatcggagt tatgataaat agctggacaa gtggaagaag
     2521 gatatttaga gtccagatgc atgctccagg gatatgggtt gaagcggacc ctgaggataa
     2581 aggacgttcg tatataaatg gtgatacaaa tggaattgaa tatgatacaa aagaggtggc
     2641 aggaagtagc gagttctatg tctatgcagg ctttcaggct aagaacagag aatttatcga
     2701 ttgtttaaaa ctaaagaaac agccaggctc aaacttttct gacgccctta agactatgga
     2761 ggtagcagaa aaaatattgg caaaggcgct attggaggat taaatatgga gtatgtaaag
     2821 ccggtaaaga tagaattata ctttatacca gttaaaaccc gtattccgct taaatttgga
     2881 ccagaaattt tgacatctgt taactgtgcc agggtccata tgtttgtctc tgataataaa
     2941 ggtaaggtta tggatggctg gggagagaca cctctgagtg ttcagtgg
//
LOCUS       DAOTKU010000182         2984 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_27102_length_2984_cov_3.332878, whole genome shotgun sequence.
ACCESSION   DAOTKU010000182 DAOTKU010000000
VERSION     DAOTKU010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27102_length_2984_cov_3.332878"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="P5310_05070"
     CDS             complement(<1..437)
                     /locus_tag="P5310_05070"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein"
                     /protein_id="HRU32765.1"
                     /translation="MKGYFMLLLHTHLPFVKGEGVWPFGEEWLYEVMYSSYIPLIKTL
                     EELHDEGIKLNVTLSITPVLLSQLMMPECIDGFRSYMLRKMELISADRDYFKHTGEID
                     LYNLTFYYRDILEERLRYFEELGGNIVNKISELAKNGAIELITS"
     gene            complement(451..975)
                     /gene="cobO"
                     /locus_tag="P5310_05075"
     CDS             complement(451..975)
                     /gene="cobO"
                     /locus_tag="P5310_05075"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011024816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HRU32766.1"
                     /translation="MWEGLVEVYTGDGKGKTTCAFGLALRALGRGKKVIIFQFIKSIS
                     SPSGEVIALKKAFPELEIIPGGMGRFIIGEPTEEDLSLAKDLYNRILLTVSSDKYDMV
                     IADEIFPAYRAGLISLEEVIELINKKSAGTELVLTGRGAPEEIIRLAHLVTEMKKIKH
                     PYEQGIKARIGIEY"
     gene            complement(960..2075)
                     /locus_tag="P5310_05080"
     CDS             complement(960..2075)
                     /locus_tag="P5310_05080"
                     /inference="COORDINATES: protein motif:HMM:NF018613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRU32767.1"
                     /translation="MRILFLTASIGTGHNKAIESVANALNIISKERCKEDVEYKIVDV
                     YNYINIALGKLVSKSYERIVNIAPNLYGYFYTLGEDSTTLKDFLHHIGVSRVKKLVED
                     YNPDVIVSSHESPSGMVAHLKQEYGLKKELIGIVTDFRAHPFWVYKEIDKYMVPNQFT
                     MSYLIKEGVSPEKIYVTGIPVDLRFTESYDKIKLRDKFHLDHNRFTILLMGSWLDIGI
                     DTLFEFLNETSLPIQVIAVSGKNKNLQEKLEKIRDKASIPVIVFGFINNVYEMMYSSD
                     IIITKPGGLISSETLAAGLPMIIVNPIPGQEEFNALYLVTEGAGIRVNKLDEVPIIVE
                     LLLNYPERISEMSNNARRIGRPRASFDIAEIILCGKD"
     gene            complement(2077..>2984)
                     /locus_tag="P5310_05085"
     CDS             complement(2077..>2984)
                     /locus_tag="P5310_05085"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HRU32768.1"
                     /translation="KSVEEVSEYLNIPPSKLIKTIIIKADGRNVALMVRGDSEVNLNK
                     VEKLLNAREISLLEDTEGSLPLGFVGPVGLENIEIIADPEVKGIKNGVVGANIQDYHY
                     KNVNIGRDFIPDRFYPIRKVKDRDLCPRCGKELVVSKGIEAGHIFKLGTKYSEPIGAM
                     FQTPTGELKPVIMGCYGIGVSRLVAASIEQNNDRNGIIWPRNIAPYDIYILPTDWANE
                     SIRNFAETLYKKLLERGYEVILDDRDERAGTKFVDADLLGIPLRITIGNKFVKSDIVE
                     IKERKSSEVEEIPSEELILKLKEVL"
BASE COUNT          882 a          706 c          418 g          978 t
ORIGIN      
        1 gatgttatga gctctatagc accgttcttt gctagctctg aaatcttatt aactatattt
       61 ccacctaact cttcaaaata tctaagcctt tcctccagta tgtctctgta gtaaaaagta
      121 agattgtata gatctatttc tccggtatgt ttaaagtaat ctctgtcagc actgataagt
      181 tccatctttc tcaacatata acttctaaaa ccatctatac attctggcat cattaactgt
      241 gagagtaaaa ctggggttat actcagagta acattcaatt ttatcccttc atcatgaagc
      301 tcttccagcg tctttatcaa tgggatataa gagctgtaca ttacctcgta aagccattcc
      361 tccccaaagg gccaaacccc ttcccccttc acaaatggga gatgagtatg aagcaaaagc
      421 ataaagtatc ccttcactag gatcacctcc ttaatattct ataccaattc tagcctttat
      481 cccctgttca tatggatgtt taattttctt catctcagta acaagatgag ccaatcttat
      541 tatctcttca ggagcccctc taccggtaag gacaagctct gtaccggcag atttcttatt
      601 tatcaattct ataacctctt ccaaagagat aagacccgcc cgataagctg gaaatatctc
      661 atctgcaata accatatcat atttatcgga agaaacggtt agaagtattc tattatatag
      721 atctttagct aaagataaat cttcttcagt tggttctcca ataataaatc ttcccatacc
      781 accaggtata atttctaact ctggaaacgc cttttttaag gcgatgactt ccccagaagg
      841 ggaagagata gattttataa actgaaatat tataaccttc ttccctctac ctaaagccct
      901 tagggcaagt ccaaaagcac atgtagtctt tccctttcca tccccagtat atacctctac
      961 taatccttcc cacatagaat aatctctgct atatcaaaac ttgctctggg tctcccaatt
     1021 ctcctggcat tattggacat ctctgatatc ctctcaggat aattcaaaag taactctact
     1081 attataggca cctcatcaag tttattaacc cttataccag ctccctcagt aacaagatat
     1141 agcgcattga attcttcttg ccctggtatg ggattaacta taatcatagg aagcccagcc
     1201 gccagagtct cagaggagat aagccctcca ggcttagtaa ttattatatc tgaagaatac
     1261 atcatctcat acacattatt tataaagcca aatacgatta ctggaatact cgctttatct
     1321 cttatctttt ccaatttctc ctgaaggttt ttattctttc ccgaaaccgc aataacctga
     1381 ataggcaagg atgtctcgtt caaaaattca aaaagggtat ctattccaat atctaaccag
     1441 cttcccataa ggagtatagt aaacctgtta tggtcaagat gaaatttgtc tctaagcttt
     1501 atcttatcat aactttcggt aaatctcaag tccacgggaa taccggttac atatattttt
     1561 tctggtgaaa caccctcctt tataagatag gacatagtaa actggttagg aaccatatat
     1621 ttatcgattt ctttgtatac ccagaaggga tgtgccctaa aatctgtcac tattccaatt
     1681 aattcttttt tcaatccgta ttcctgtttt agatgggcaa ccatccccga gggagattca
     1741 tgagaagaga ctatcacatc tggattataa tcctctacaa gctttttcac tctacttacc
     1801 cctatatgat gtaaaaagtc cttgagagta gtggagtctt cccccagggt atagaaataa
     1861 ccatacagat tgggggcaat atttactatc ctctcataac tcttagacac aagtttaccc
     1921 agggcgatat ttatatagtt atacacatct actatcttat attcaacatc ttccttacat
     1981 ctttccttgc taattatatt cagggcatta gctacagact ctatagcctt attatgccct
     2041 gtccctatgc tggcggtgag aaagagtatc ctcatattag agaacctctt tcaactttaa
     2101 tattaattct tctgaaggaa tctcttccac ttcggaactt ttacgttctt taatctctac
     2161 tatatccgac ttaacaaatt tattaccaat agtaatacgg agaggtattc caagaaggtc
     2221 agcatctaca aacttggttc ctgctctttc gtctctatca tctaaaataa cctcatatcc
     2281 cctctctaac aattttttat acagggtctc ggcaaagttc cttatagatt cgttagccca
     2341 atcagtgggc aatatatata tatcatatgg agctatattc ctcggccata taataccatt
     2401 tctatcatta ttttgctcta tactggcagc aaccaatctg ctcactccta tcccataaca
     2461 tcccataata actggtttta attctcccgt tggagtttga aacatggcac ctataggctc
     2521 actatattta gtacccagtt taaatatatg accagcctct atcccctttg acactacaag
     2581 ttctttacca catctcggac ataaatctct atcttttact ttccttatag gatagaatct
     2641 atccgggatg aaatctctcc ctatattcac attcttatag tgataatcct gaatattagc
     2701 tcccaccaca ccattcttaa taccctttac ctccggatca gcgataatct ctatattctc
     2761 cagacctaca ggtcctacaa aacccagagg taaagagcct tcagtatctt ccaaaagaga
     2821 aatctccctg gcattcaata gcttttccac tttattgagg tttacctcac tatcccctct
     2881 aaccatcaga gcaacatttc taccatctgc ctttattatg atggtcttaa taagcttgga
     2941 tggagggatg tttagatatt cagacacctc ttccacactc ttta
//
LOCUS       DAOTKU010000183         2979 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_27153_length_2979_cov_3.418947, whole genome shotgun sequence.
ACCESSION   DAOTKU010000183 DAOTKU010000000
VERSION     DAOTKU010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2979
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27153_length_2979_cov_3.418947"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="P5310_05090"
     CDS             <1..482
                     /locus_tag="P5310_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32769.1"
                     /translation="IEAFLSGLAYIGFHGYPITDRYGVRQRFISIVTDLPLSNDPVYD
                     GEILCEQCDKPCINACPTSAITEDSLKLIHFEGREFKIPNLNTFACDWAKRYCLVGEE
                     GPSYWNVDINIPVPKEESVEDVLNSVSKYPWGVQKLHINIVEECLRRCRAVGRSEKG"
     gene            637..1734
                     /locus_tag="P5310_05095"
     CDS             637..1734
                     /locus_tag="P5310_05095"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRU32770.1"
                     /translation="MPSYVFCISIDDYNYMTEDDRYRSKNSLKEAIAQILQGKEYSLI
                     FAHQNCYYLNGHITEIQLGEESVENYSYILLNLARKIRGKVKEIKEISLTTGIDFCPH
                     PSIQKWKEISQHAIVAQRRKFFEGKDRDYFFLSEGSNNSPSFSLLSKSYFEVQQKLLN
                     AIITGDLKEAEELSVGLVEDIFKYNIGRLFYLRIRLIEAGILTVCNLIELGLPEPEIF
                     QILIEFSEKINSLHDFINLAETFYSLTRNLTELAVKLDYKLNPIILKIKELVESSEDL
                     SDLNLNKVSRMVNANYSYVSRLFKKELGVSFTEYINRERMKRAIPLIFDKQKSIEDIA
                     INAGFRDMQHFERVFKNFYNCSPLHYRKNNL"
     gene            complement(2163..>2979)
                     /locus_tag="P5310_05100"
     CDS             complement(2163..>2979)
                     /locus_tag="P5310_05100"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HRU32771.1"
                     /translation="LSLIKGTGPGGRIVEEDVKDYIESHKERVTTQPPPVSTPVVEVT
                     PLSKEEPISSVRREIASKLSKSYREAVHITLEFSADVTGIVEIRERLKEKAEKNKLPV
                     PTFNDIFVRIVSLVLKDYPTLNANFDGEKLTIFNTVNMGVAVAVKEGLLVPVVKEAEK
                     KNVFEIAKITGDLVNRTREGKILPDELSGGTFTITNLGMFGIDFFTPIINPPQVAILG
                     IGRIKEVLKPGNAVVSEVVLSLSVDHRVVDGAPAARFLKDIGEALKDPLLFLI"
BASE COUNT          936 a          527 c          578 g          938 t
ORIGIN      
        1 taattgaggc atttctaagt ggattagctt atataggttt ccatgggtat cctataactg
       61 ataggtatgg agtaaggcag agatttatct ctatcgttac cgatttacct ttatctaatg
      121 atcctgtata tgatggagaa attctctgtg aacagtgcga caaaccatgt atcaatgcct
      181 gtcccacttc tgcgataaca gaggatagct taaaacttat acactttgaa ggaagagagt
      241 ttaaaattcc taatctaaat acttttgcct gtgactgggc aaagaggtac tgtttggttg
      301 gagaggaagg tccatcctac tggaatgttg acattaatat tccggtacct aaagaagaga
      361 gtgtagaaga tgtcttgaat tctgtaagta agtacccatg gggtgtccaa aaattacata
      421 ttaacattgt agaggaatgt ctaaggagat gtagagctgt gggtagatca gaaaagggat
      481 agaagaagat gaattatcat ggggattaaa ttagagagtc tagcattatc taatttttat
      541 caaattgttt gggatatatt tatatacggt caatactata gtggtttaga taagttggac
      601 cttgagggac taccggatct attaggatta agcaaagtgc ctagctatgt attttgtatc
      661 tctatagatg actataacta tatgacagaa gatgatcgct atagatcaaa aaattctctc
      721 aaggaagcta tagcccagat attacaagga aaggagtatt cccttatatt tgcccaccaa
      781 aattgctact atctgaatgg gcatattaca gaaattcagc taggggaaga aagtgtggaa
      841 aactatagct atatcttact gaatttggca agaaagataa ggggtaaagt aaaagaaatt
      901 aaagagatat ctcttacaac aggtatagat ttctgccctc atccttcgat acagaagtgg
      961 aaagaaatat ctcaacatgc aatagtggca cagagaagaa aattctttga gggaaaagac
     1021 agagattatt tcttcttatc agaagggtct aataactctc cttctttctc cttactatct
     1081 aaatcatatt tcgaagttca acagaagctt ctcaatgcta tcatcactgg tgacttaaaa
     1141 gaagctgaag aactttcagt tggattggta gaagacatat tcaaatataa tattggaaga
     1201 ctattctatc tccggataag actcatagag gcgggcattc tgacagtatg taacttgata
     1261 gaattaggac tgccagagcc tgaaatcttt caaatattaa tagaatttag cgagaagata
     1321 aattctctac acgactttat aaaccttgca gaaacatttt atagtttaac aagaaattta
     1381 acagaattag cagtaaaact tgattacaaa ctgaatccaa ttatattgaa gattaaggaa
     1441 ctggtcgaat cttctgagga cctttctgat cttaatttaa acaaagtatc aagaatggtc
     1501 aatgcaaact actcgtatgt aagtcgccta tttaaaaagg agttgggagt ttcgtttacc
     1561 gaatatatta acagagaacg aatgaagaga gctatccctc tcatctttga taaacagaag
     1621 agtatagaag atattgctat aaatgctgga ttcagggata tgcagcactt tgaaagagtg
     1681 ttcaaaaatt tctataactg ttcacctcta cattatcgta aaaacaatct ctagacttaa
     1741 gggcttagtt taaagatttg acgagtaatc tctaagacta aagatcctct tcctcgtcgt
     1801 atattgaata ctgaggaagg ggatcctctt ttgcctctat aaccttaggc ttttcaaatt
     1861 cggaataatc tttaattata tcctttctca ttcttcccaa agtagtgacg cgtgataggt
     1921 gagacgtaag gatgatgtgt aattaacagg attaatttca tatgtctcat tttcctctac
     1981 atcctgaggg agaagtaaga gtataaaata gatttagaaa atccattaac gctggtaatc
     2041 ttttttatct gttgtattta tagtaattga caattttaat ttatgatata atttacaggg
     2101 gactatcctg agcccgactt taataaaaga ttgatgggcg tggatggtcc ccctttaatt
     2161 acttatataa gaaagagtag cggatctttc agtgcttcac caatgtcttt taaaaatctt
     2221 gctgctggag cgccatccac cactctgtga tcgactgaga ggctcagcac aacctcgctg
     2281 actacagcat taccaggttt aagtacctcc ttaatcctgc caatacccag gattgcaacc
     2341 tgaggtggat ttattatcgg tgtaaagaag tctattccga acatcccgag gtttgtaatt
     2401 gtaaatgttc ctccggataa ttcatccgga agaatcttcc cctcccttgt tctatttacc
     2461 aggtccccgg ttatctttgc tatctcaaag acatttttct tctcagcctc tttgaccaca
     2521 gggacaagga gaccttcctt taccgcaacc gctactccca tatttacagt attgaatatt
     2581 gtaagttttt caccatcaaa attggcattt aaggttgggt agtctttaag cacaagggag
     2641 actatcctta caaatatgtc attgaatgtc ggaactggga gtttattttt ctctgccttc
     2701 tctttaagcc tctctctgat ctcgacaata cctgttacat cagcagaaaa ctccagcgtg
     2761 atgtgaacag cctctctata actcttcgat aattttgatg ctatctctct ccttactgag
     2821 gagattggtt cttcttttga aagtggagtg acttcaacta caggtgtaga gactggaggg
     2881 ggttgagtgg taaccctttc tttgtgggat tctatataat ctttgacatc ctcttctaca
     2941 atcctccctc ctggtccggt cccctttata agggacagg
//
LOCUS       DAOTKU010000184         2967 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_27282_length_2967_cov_3.620879, whole genome shotgun sequence.
ACCESSION   DAOTKU010000184 DAOTKU010000000
VERSION     DAOTKU010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27282_length_2967_cov_3.620879"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            326..2281
                     /locus_tag="P5310_05105"
     CDS             326..2281
                     /locus_tag="P5310_05105"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRU32772.1"
                     /translation="MKNSFKLISILVVLGIIFSGFSTAFAQKAPSVFQYSTPIEYQKA
                     TGKRIAVFQEAPQLTEMVKSGKILPVKERLPKEPLVIVPVEEVGRYGGTWRRAAIGIA
                     DASIIETKLTAEGLVRTNPDGSKIVPNLAKRWEISRDGRVYTFYLREGLRWSDGEPFT
                     ADDITFWFNDIISNKELTPITPAWLTTAGKPVSVEKVDKYVVRFTFQQPNGVFLWQLS
                     YDGIYNKPFAPAHYLKQFHPSYVSKEELKKKAKEAGFNFWYQLFSNKNSWQNTEKPTI
                     NAWMVSRITSTQVICERNPYYWKVDTAGNQLPYIDKIVMDIVQDAEILNMRATAGELD
                     MQERHIYLSNYTLFMENREKGNYRVLKWTTASGADPMLYVNQTVQDPVLRKIFQDVRF
                     RQALSLAINREEINKLFYFGLGKPRQASLISQSPYYDPEWEKAYAEYDLERANKLLDE
                     MGLKKGPDSYRIRPDGKTLAITLEFTAFPGATTADVIALIAKYWEKLGIKVAVKQIDR
                     SLWETRYSGNQLEASAWSLDKSLNPLLNITWFVLVGSQQWAPAWGTWIATGGKSGMEP
                     PADIKKISQLWEKAKTTTNSDIRDRYLKEIFNIHKKNLWMIGTVGELPQILIVKNNFR
                     NVPEGLISDDSLFSPANAHPEQFFIKE"
     gene            2322..>2967
                     /locus_tag="P5310_05110"
     CDS             2322..>2967
                     /locus_tag="P5310_05110"
                     /inference="COORDINATES: protein motif:HMM:NF025851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HRU32773.1"
                     /translation="MSDEVNTDLLTKRLLYRQPARRWLEALPIGNGRLGGMIFGGILE
                     DRIQLNEDSVYYGGPKDRNNPFAFKNLDRIRELLKEKEISEAESLIRTTMLGIPRYNT
                     AYQPLGDLFIRSLGYKKEPESYLRELDLNLGIVRVIYKIDGVTYIREYFSSAVDDVLV
                     IRLSADKPEMISSLLNIVRRPFDSGTRILPDNRLMLKDKCGDGVEFCLIVQAIAE"
BASE COUNT          980 a          486 c          644 g          857 t
ORIGIN      
        1 aagatagtaa acaagaaata tcataactat tataaatcca gtaactctta ataatatttt
       61 gtctctcatt acttactgag gtataccacc ggagactatc ttttactcta tccggtcttg
      121 ctggactaac tatattcaac ccttcaataa tatttatccc tctcaaatat attatatcat
      181 gtgttatcgt tctgtgatat tgtcacacgg gtaacataaa tgccgtaacg cgtgataggt
      241 gatgcgtggc gaatagaatt tgacttttaa tattattgtt gtataatgtt actgataaca
      301 taaattgatg tgagggggtg aacgtatgaa aaattcattt aagttaattt ctatccttgt
      361 agtgcttggt attatcttta gtggattttc taccgctttt gctcaaaagg ctccttccgt
      421 ttttcagtat agtactccta tagaatatca gaaagctacc ggaaagagaa ttgcagtatt
      481 tcaggaggca ccgcagttaa ctgaaatggt aaaatcgggt aagatcttac cagtaaaaga
      541 gaggctacca aaagaacctc ttgttattgt tccggtagaa gaggtcggtc ggtatggagg
      601 aacctggaga agagctgcta taggcatagc agatgcttct attattgaaa caaaactaac
      661 agctgaaggt ttagtaagaa ctaacccgga tggaagtaag atagttccga accttgctaa
      721 gaggtgggag atatcacgtg atggaagggt atatacattt tatctcagag agggactaag
      781 atggtctgat ggggagcctt ttactgcgga tgatataacc ttctggttta atgacatcat
      841 atcaaataag gaattaactc ccattactcc tgcctggtta accacagcag gaaaacctgt
      901 ttcggtagaa aaagtagata aatatgttgt caggtttact tttcaacagc caaatggagt
      961 tttcttatgg cagttatctt atgatggtat ctataacaaa ccttttgccc cagcccatta
     1021 ccttaagcaa ttccatccca gttatgtttc taaagaagag ttaaagaaaa aagctaaaga
     1081 agcaggattt aacttctggt atcaactctt cagtaataaa aactcctggc aaaatacaga
     1141 aaaacccacc ataaatgcct ggatggtcag cagaataaca agtacccagg ttatatgtga
     1201 aaggaatcct tactactgga aggttgatac cgccggcaat cagctcccat acatagataa
     1261 aattgttatg gatatagtac aggatgctga gatcctgaat atgagagcga cagctggaga
     1321 acttgatatg caggagaggc atatatacct gtcgaattat acgttattta tggaaaatag
     1381 agaaaaaggt aattataggg tgttaaaatg gacaactgca tctggggctg atcctatgct
     1441 ctatgtaaac cagacagtgc aggatccggt attaagaaag atcttccaag atgttagatt
     1501 caggcaagca ttgtcactgg ctataaatag agaggagatt aataaacttt tctattttgg
     1561 tttaggaaaa ccaagacagg cttcgcttat aagccaatct ccctactatg accctgagtg
     1621 ggaaaaggcg tatgcagagt atgatctgga aagagctaac aaacttctgg atgagatggg
     1681 acttaagaaa ggtcctgata gttatagaat aagaccagac ggaaagactt tagctattac
     1741 attagagttt actgcatttc caggagctac aaccgctgat gtaatagcat taattgctaa
     1801 atattgggag aaacttggaa tcaaagtggc tgtaaaacag atagatcgtt ctttatggga
     1861 aacccgttat tctggaaacc agttagaagc tagtgcatgg agtcttgata agagtctgaa
     1921 ccctctactt aatattacat ggtttgttct tgttggttca cagcagtggg ctcctgcatg
     1981 ggggacatgg attgctaccg gaggtaaaag tggaatggag cctcctgcag atataaagaa
     2041 gatatcgcaa ctatgggaaa aggcaaagac aactacaaat agtgatataa gggatagata
     2101 tctcaaagag atatttaata tccataagaa gaatctctgg atgataggaa ctgttggaga
     2161 gcttccccaa atccttatcg taaagaataa cttcagaaat gttcctgaag gtctgatatc
     2221 agatgattct ctcttttcac ccgcaaatgc tcatcctgaa cagttcttta taaaagaata
     2281 atataagggg ggtgaatcct ccctcttagg aggttaatta gatgtctgat gaggtaaata
     2341 cagatcttct tacgaagaga ctcttgtaca ggcaacctgc aagacgttgg ttagaggcgc
     2401 ttcccattgg gaatggaaga cttgggggga tgatatttgg aggaatttta gaggatagga
     2461 ttcagctaaa tgaagattct gtgtattatg gaggtccaaa ggatagaaat aatccttttg
     2521 ccttcaaaaa tctggatagg ataagagagc tattgaaaga aaaagagatc tctgaagctg
     2581 agtcccttat aagaactact atgctgggta ttccaaggta taacacagct taccagccat
     2641 taggagatct ttttataaga tccttaggct ataaaaaaga accagaatca tatctaagag
     2701 agcttgatct caacctaggt atagtacgtg tgatctataa aatagatgga gttacttata
     2761 taagagagta tttctccagt gcagtggatg atgtcctggt tataagatta agtgctgata
     2821 aacccgagat gatatcctct cttttgaata tagttagaag accatttgat tcaggaacaa
     2881 gaatccttcc agataataga ttaatgctta aagataaatg tggggatggg gtagaatttt
     2941 gtctcattgt tcaagctatt gcagaag
//
LOCUS       DAOTKU010000185         2960 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_27358_length_2960_cov_3.237177, whole genome shotgun sequence.
ACCESSION   DAOTKU010000185 DAOTKU010000000
VERSION     DAOTKU010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27358_length_2960_cov_3.237177"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="P5310_05115"
     CDS             <1..391
                     /locus_tag="P5310_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HRU32774.1"
                     /translation="VNRNKRVDLTEGIESVVSTDEIKYVRSAYNDVKISEEVMDYLLD
                     IIEKTRNMDMVQLGVSPRGTIALFKASQAYAAINGRDYIIPEDIKRMAPYVLNHRVIL
                     KGLTKDRNIYSFINSVLETVRVPVEEV"
     gene            395..1543
                     /locus_tag="P5310_05120"
     CDS             395..1543
                     /locus_tag="P5310_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRU32775.1"
                     /translation="MAYLILFLVVSGIVLNRLSRRYTLLGVTYSREISKRLLEIGEDF
                     HITTVVENRKFLPVTFLQIIERFPAGIEYRFRADQSKSREFLYHTITMFLLPFQRIKR
                     VYTATCNKRGRYIFEDVSIIGGDLLGLNTFVKSIRYSQEIVVLPEPVDLENELIPYGN
                     YNGNLSVQRWIIDDPTMIIGIKEYTGLESAKTIHWPSSLRTGNLMVKKFDFTMENTAM
                     IILNVECDKPFWVGVKPDDIERCISITRGVMEKFESMGIPYGFATNSILYGLPASRNI
                     IYPSSGPAHLEELLECLGRVSYSISLTFEELLLSLNTRDIKCSTYVIITPEVLESYVE
                     PINNLGSELEEVILISIKDVNLEFISQDVLKYLDGGVEYVYTIPQDNK"
     gene            1512..2792
                     /locus_tag="P5310_05125"
     CDS             1512..2792
                     /locus_tag="P5310_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32776.1"
                     /translation="MYIRYLKIISNLSLAFGLFGFVFSLIGVLSNTELLYLWLILSAI
                     GLHRLSSKKIFTVILSILIFLPLFFFRGTLDRFFIIVAGVYSLYLISRLIRRVTYGGV
                     KEEFEKATPIVIILTLLSIALSFFFPGKSSLFNNNVFPYLVVYLVSSVVLLRTLRYAE
                     YSPEGGKEMNKINMVYSFSIIGVSFLLSLPVIRSVIWHIISSAFTYVTGAFVFAVTWI
                     VYAVFFLFDKIMALFHRIFPARDGELSGMNPGELPPGMMNMPNLGKAKELVEDKAESS
                     SLMDHILIIIAGIVILLVLAYVISILLRRYEYRQKPKEEDYIETKEFITPGKDGDSNI
                     VKKILARLRPKDPVEKIRLYYKRYLLNCKEKGISLDDSDTTLDIYSKSKESFNKDIIS
                     KMREIYIQVRYGRINPDSKTVKYFITLYRKLNSR"
BASE COUNT          928 a          493 c          628 g          911 t
ORIGIN      
        1 agtaaataga aacaagagag tggacctcac tgaagggata gagagtgtgg tctctactga
       61 tgaaatcaaa tatgttagaa gtgcttataa tgacgtaaag atctctgaag aagttatgga
      121 ctacctcttg gatatcatag aaaagacaag aaatatggat atggtccaac tgggggtaag
      181 tccaagggga accatagctc tgtttaaagc gagtcaggct tatgcagcga taaatggaag
      241 agactatata atccctgagg atataaagag gatggctcca tatgtactga atcatagagt
      301 tatactgaaa ggtcttacca aagatagaaa tatctattca tttataaatt ctgtcctgga
      361 aacagtaaga gtaccggtgg aagaggtatg attggtggct tatctcatac tctttcttgt
      421 agtttcaggc attgtactga atagattatc caggagatat actctcctgg gtgtcacata
      481 tagcagagaa atctccaaaa gactcctgga gataggagag gattttcata taactaccgt
      541 tgtagagaac agaaagtttc tccctgtaac ctttttacag attatagaga gatttcctgc
      601 aggtatagag tacagattta gagcagacca gtctaagagt agagagtttt tgtatcatac
      661 aattaccatg tttttgctac ctttccagag gataaagagg gtttatacag caacctgtaa
      721 taagaggggt agatatatct ttgaagatgt atctataatt ggcggggatt tattgggatt
      781 aaacaccttt gtaaagagca taagatacag tcaggagatt gtagttttac ctgagcctgt
      841 agacctggag aatgaactta tcccttacgg aaactacaat ggaaatctat ccgttcagag
      901 atggattata gatgacccaa cgatgataat aggtataaaa gagtataccg gtttagagtc
      961 agcaaagacc atacactggc catcctcatt gagaacagga aatttgatgg taaagaaatt
     1021 tgattttacc atggagaata ctgcgatgat aatcctgaat gtggagtgtg ataagccttt
     1081 ctgggtaggt gtaaaacctg acgatataga aagatgtatc tccataacca gaggtgttat
     1141 ggagaagttc gagtctatgg ggataccata cggtttcgcc acaaactcca ttttgtatgg
     1201 attacctgca agcagaaata taatctatcc atcttctggt ccagcccatc tggaggagct
     1261 gttagaatgt ttgggtagag tttcttacag tataagccta acatttgagg aactgctatt
     1321 gagcttaaat accagagata taaaatgttc tacatatgtg attataacac cagaggtcct
     1381 ggagtcttat gttgagccta taaataatct tggctcagaa ttagaagagg ttattttgat
     1441 atcaattaag gatgtaaacc tggaatttat ctctcaggat gttcttaaat atctggatgg
     1501 tggagttgag tatgtatata cgatacctca agataataag taacttgtca ctggcatttg
     1561 gcttatttgg ttttgtcttt agtcttatag gtgtactcag taatactgaa ctcctctatc
     1621 tctggttaat attaagtgct attggcctac atagactttc ctccaaaaag atatttactg
     1681 ttatcctctc catactgata tttcttcccc tattcttttt tagaggcact ttagacaggt
     1741 tttttataat tgttgcaggt gtatactcgc tgtatctaat atctagattg ataaggagag
     1801 tgacatatgg aggtgtcaaa gaagaatttg aaaaggctac ccctatagtt atcatactga
     1861 cgcttctctc aatagcttta tcttttttct ttcctggtaa atccagtcta ttcaataata
     1921 atgtatttcc atacctggtg gtatatcttg tgtcttctgt cgttcttttg agaacattga
     1981 gatatgcgga atatagccca gaaggtggga aagagatgaa taagataaac atggtatact
     2041 cattctctat tatcggtgtc tcttttctgc tgagtttacc tgttataagg tccgttatat
     2101 ggcatataat atcttctgct tttacctatg ttacaggtgc atttgtcttt gcggtaacct
     2161 ggattgtata tgctgtgttt ttcctctttg ataagattat ggcactgttt cacaggatat
     2221 ttccggcaag ggatggagaa ctatcaggga tgaatcccgg ggagcttcct cccgggatga
     2281 tgaatatgcc gaatctggga aaagctaaag agctggtgga ggataaagct gaatcttctt
     2341 ctctaatgga ccatatcctt ataatcattg ccggtatagt aatactgctt gttctggcat
     2401 atgtaatatc tatactcctc aggagatacg aatatcgaca gaaaccaaaa gaggaagatt
     2461 atatagagac aaaggaattt ataaccccgg gaaaagatgg ggactcaaat attgttaaaa
     2521 aaatcctggc aaggctcaga cccaaagacc cggtagaaaa gatcagactc tactataaaa
     2581 gatatctact gaattgtaaa gaaaagggga tatcgctgga tgatagcgat accacacttg
     2641 atatatattc gaagtctaaa gaatcattta ataaagatat tatttccaag atgagagaga
     2701 tttatataca ggtaagatat ggcaggataa atcctgacag taagactgta aaatacttta
     2761 tcaccctata caggaagctt aactcaagat aacgtttcta aattctgcct tctattttta
     2821 aaatagcctg tttcctgcta tcgttctgtg ataggtacgt agcgcgtgac gcgtgatgag
     2881 taacgggaac ttaccctttt ccctctcctg tcccccacct ctatcctccc ccgaaatagg
     2941 ggaggaagaa aatggattat
//
LOCUS       DAOTKU010000186         2903 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_27987_length_2903_cov_3.476475, whole genome shotgun sequence.
ACCESSION   DAOTKU010000186 DAOTKU010000000
VERSION     DAOTKU010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27987_length_2903_cov_3.476475"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="P5310_05130"
     CDS             complement(<1..581)
                     /locus_tag="P5310_05130"
                     /inference="COORDINATES: protein motif:HMM:NF013296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HRU32777.1"
                     /translation="MEITLKDILPSAYREHRAILHCNIHTLEDIEGVVEAGYELDLPV
                     IVACTPRVLNTIGGRSIVGVYRSLADYCSTPVVLHLDHTTELRDIWRAISSGFTSVMI
                     DGSRLPYEENVQLTRTVVEVARAAGISVEGEIGAVPGKEDDLKDYSKDLTDPDMAYSF
                     VLETEVDALAVSIGSAHGFYKELPKLDFERLGS"
     gene            complement(565..1491)
                     /locus_tag="P5310_05135"
     CDS             complement(565..1491)
                     /locus_tag="P5310_05135"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018212683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HRU32778.1"
                     /translation="MEVLRSKDLLDTDALTEEEIRLIIETAFRIKNEGLKKLPLSNDI
                     ISLLFFEPSTRTRNSFEIAGKKLGAIVVSMDTSGSSLVKGESALNTVRTIQAMGVNYF
                     VIRHRESGFPYPIAEKVSASIINAGDGMRGHPTQALLDAMTMIEARGDIKNRKVIIVG
                     DILHSRVARSNIWLLQKLGAKVTLAGPPEFLPEDFRQFDVRISHSLDDELKDTDIIMG
                     LRIQRERMEKMNISVDGYVKRYQINSERVSRAKPDLIILHPGPVNVGIEMTADVLYSE
                     KSLVERQVTNGVFIRMAILSLLKETRDYGDNT"
     gene            1642..2673
                     /locus_tag="P5310_05140"
     CDS             1642..2673
                     /locus_tag="P5310_05140"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HRU32779.1"
                     /translation="MLAAVLEGVRQMELKDVPVPGIKRNEVLVRVKACGICQTDYSAY
                     TGRRTNWTPPIILGHEISGVVEDVGDEVDSWKLGDEVILSPVISCGECDNCRLGLGHY
                     CRNGKVIGGEGQKIVLPGGFAEYVAVPTSVLYKKPANATFESAALSEPLAGSYKGMIE
                     YSNLRLGEDVVIIGAGAMGLLLLELAVAGGAGNTIVIDVVDARLKKAKELGATHTINS
                     KEVDPREAVYDIIPNGPDIVFEAAGVLEAAKLSFGLTRRGTRVNMFGVIIPGEIPVSP
                     AEIHFNETRVDASFSVNPRVMTKAISLIEKGKVDPGKIVTHRFNLTEIDKALEAMELP
                     DRIKVVINP"
BASE COUNT          835 a          609 c          610 g          849 t
ORIGIN      
        1 atactgccta atctttcaaa gtctaattta ggtagctcct tataaaatcc gtgggcagaa
       61 ccgatagata cagctaacgc atccacctct gtctctaaga caaaagaata agccatatcc
      121 ggatcagtca aatctttgct gtaatctttt aaatcatcct cttttccagg gactgctcct
      181 atctccccct ccacagaaat accagccgcc ctagcaacct ccaccacagt gcgtgtaagc
      241 tgcacattct cctcatacgg taacctggaa ccatcaatca tcaccgatgt gaaacctgaa
      301 cttatagccc tccatatatc tctgagctct gtggtatgat ccagatggag tactactggt
      361 gtactgcagt aatcagctaa agatctgtat acgcctacta tactcctccc accaatggta
      421 ttgagtactc ttggtgtgca ggctactata accggcaggt ccaattcgta gcctgcctct
      481 acaaccccct ctatgtcttc cagcgtatgt atattacaat gcagtatcgc cctgtgttct
      541 ctgtatgcag aggggagtat atctttaagt gttatctcca taatccctag tctcctttaa
      601 aagacttaat atagccattc ttataaagac accatttgtt acctgcctct ccaccagaga
      661 cttttcagag taaagtacat ccgctgtcat ctctatccca acatttaccg gtccaggatg
      721 aagaattata aggtctggtt ttgccctgga gactctctcg ctatttatct ggtatctctt
      781 cacatatccg tctacagaga tattcatctt ctccatcctt tccctctgga ttcttaatcc
      841 cataatgata tcggtatctt ttaactcatc gtcaagagag tgacttattc taacatcaaa
      901 ctgcctgaaa tcttcaggca aaaactctgg tggtcctgca agagtcacct tggctcccaa
      961 tttttgtaga agccatatgt ttgatctggc aaccctgcta tggagaatat ctcccacaat
     1021 aataaccttt ctattcttta tatctcccct tgcctctatc attgtcatag catccaggag
     1081 agcctgagta ggatgccctc tcatcccatc tcctgcattt attatagatg cagacacttt
     1141 ttccgctatt gggtagggaa atccagactc tctatgtctt attacgaaat agtttacccc
     1201 catagcttgg atggttctta ccgtatttaa tgcggattct cctttgacca gactgcttcc
     1261 cgaggtatcc atagatacca ctatggcacc aagttttttc cctgctatct caaaagaatt
     1321 cctggttctt gtactgggtt caaagaagag gagagaaata atatcattgg aaaggggaag
     1381 tttcttaagt ccttcattct taatcctgaa tgcggtctct attataagtc ttatctcttc
     1441 ttctgtaagg gcatccgtat ctaataagtc cttactccgt aatacttcca ctaggatcac
     1501 ctcactcttt aggcgatccg gcaaaacctt ttcggactca ccggaccgat taaaaggtta
     1561 agggtataat aacaaaaaaa gacaaaaaag tcaaaaacct gtataattta acttagaatt
     1621 aactgaaagg aggagattga aatgttagca gctgtattgg agggagtaag gcagatggaa
     1681 ctgaaagatg ttcctgtgcc aggtattaaa agaaatgagg ttcttgttag agtaaaagcc
     1741 tgtggaatct gtcagacgga ctattctgcc tatacaggga gaagaactaa ctggacccca
     1801 cccataattc ttggtcatga gatatcaggg gtggtggaag atgtgggtga tgaggtggat
     1861 agctggaaac taggggatga agttatatta agtcctgtta tctcctgcgg agagtgtgat
     1921 aactgcagac tgggtctggg acactactgc aggaatggga aggttatagg tggagaaggt
     1981 cagaagattg ttctgccagg agggtttgca gagtatgttg ctgttcccac cagtgtcctc
     2041 tataagaaac cagcaaatgc aacctttgag tctgccgcac tttctgaacc tctcgccggt
     2101 tcgtataaag gcatgattga gtattccaat ctgagattag gagaggatgt agttatcatt
     2161 ggagctggag caatgggatt acttcttctt gagcttgcag tggctggtgg agctggaaac
     2221 acaatcgtta tagatgtggt tgatgcaaga ctaaagaaag caaaagagct gggggctaca
     2281 catactatta attctaagga ggtagacccc agagaggctg tctatgatat tatacccaat
     2341 ggtccggata tagtatttga agcggcaggg gtgttagaag cggctaaact gtcttttgga
     2401 ctaaccagaa gaggaaccag agtaaatatg tttggtgtca ttataccagg tgaaattcct
     2461 gtatctccag ccgagatcca ctttaatgag accagagtag atgcgtcctt ctctgtaaat
     2521 ccaagagtta tgacaaaggc tatatctctc atagagaaag ggaaggtgga ccctgggaaa
     2581 atagttacgc atagattcaa tcttacagag atagataaag cattagaagc tatggaacta
     2641 ccagatcgta taaaggttgt tataaatccg taaataaaaa aatagagccc cgagggggct
     2701 ctatagggag gtgggttatg agaggttacc tggtgttggt gtcttttgac atttatattc
     2761 tagacgtgta gtgttaagaa attattaaga atttattaat cttctgttaa gattttatat
     2821 gctgattgaa gtgtatacca gaagggatta accaccaggg atatacaaaa ctacataatg
     2881 gagatataca gatataatct ttc
//
LOCUS       DAOTKU010000187         2897 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_28055_length_2897_cov_4.772343, whole genome shotgun sequence.
ACCESSION   DAOTKU010000187 DAOTKU010000000
VERSION     DAOTKU010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28055_length_2897_cov_4.772343"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            16..543
                     /locus_tag="P5310_05145"
     CDS             16..543
                     /locus_tag="P5310_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRU32780.1"
                     /translation="MWIWIAVFFASIVLFFVWILWTELFGAGWSPTPVETVKAMLRLG
                     DVKPGDMLYDLGAGDGRIVTIAARDFGARAIGYEIDPIRFIISWLRIVFSGVSGKATV
                     RFKNFYNADLSPATVVTLFLRQHTNENLREKLERELAPGTRVLTYYWTFDKWKPVKVD
                     RRVDIYLYIIGISNS"
     gene            complement(733..1170)
                     /locus_tag="P5310_05150"
     CDS             complement(733..1170)
                     /locus_tag="P5310_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007062923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HRU32781.1"
                     /translation="MSKVDSVIETFKSGFNCCQSIISVYGPDLGLDKDLALLISSGFG
                     GGLGRSGEVCGAVTGAIMVLGLKSGYTTFSDEAKEEIYGIVRDFIARYKRKNNSILCR
                     DLLGVDMGTPEGKKLVHEKGLTATLCPKFIRDSAEILEEMLGK"
     gene            1294..>2897
                     /locus_tag="P5310_05155"
     CDS             1294..>2897
                     /locus_tag="P5310_05155"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HRU32782.1"
                     /translation="MLNEKEYYDKVYGCWLGKNAGGTLGTPLESGWGKEEMFDVWWYP
                     KLQEGGLPNDDLELQLIWLQALEDRGLDITARDLAEYWLDCIAYNFDEYGLNKTNLKK
                     GLVPPVSGYFNNWFKHCMGSPIRSEIWACIAPGLPNVAARYAYEDAIVDHAGGESVYG
                     EIFNAVLESSAFFISDKIKLLEIGLSFIPESCETAKAIRNAMIAYDRGMDWKEARNFV
                     LKNSYSPIAQYSPVNLGFQTIGLLYGDDFGDAICKAVNCGYDTDCTGATLGSILGIIL
                     GKDGLPSKWIEPLSDKIATNASWGGIKNVREPKDLDELTRRVCRIGKKIMALHGEESD
                     SFNPEEETKKLWYIPPTRVDYDLKTVVISLDYIDNPAIQFEKAKVFNLILKNPHPETI
                     EAKISCRFPEGWDINPEDKKIIIQPDEEITIPYTVSVRDIKILGTSNRGSISISVKER
                     PQIPDIPVVYTGSNRWLISQRFDGDINTKTPIEDNLNPGGISDGWMITNFDDNELLIE
                     PHFEGKPGIVYLRHYIYSAFPRPVRLG"
BASE COUNT          879 a          527 c          649 g          842 t
ORIGIN      
        1 cttgaggaat aaactatgtg gatctggata gcagtatttt ttgcctccat agtcctgttt
       61 tttgtatgga tattatggac agagcttttt ggtgctggat ggagtccaac acctgtagaa
      121 acggtaaagg ctatgttgag gttaggggat gtaaaacccg gtgatatgct ttatgacctg
      181 ggagcagggg atggcaggat agtcaccatt gctgctagag attttggagc cagagctata
      241 ggttacgaga tagacccaat ccggtttatt ataagctggc tcagaattgt cttttctgga
      301 gtctcgggta aggcaactgt aagatttaag aatttctata atgcagatct cagtcctgca
      361 accgtggtaa ctctattttt gagacagcac accaacgaaa atctgagaga gaaactggag
      421 cgagagcttg cacccgggac tcgtgtgctc acttattact ggacttttga taagtggaag
      481 ccggtaaaag tggataggcg tgtggatata tatctgtata taattggtat atctaactct
      541 taatcctctt aagcaagagt ctattgttaa ccttggtaga attgatgatc ttggcacttc
      601 taagctttac gtaagtttga caccttagac gtaggcacgt gaatctattc tattttatct
      661 atacctataa tcttcggtgg aactctaccc ccctttctgg ggtctctttt ccggaccggt
      721 aagttatcct tcttactttc ccaacatctc ttccagtatt tctgccgaat ccctgatgaa
      781 cttagggcat aaagtagcag ttaatccttt ttcgtgaact aattttttgc cttctggagt
      841 acccatgtca acaccaagca gatctctgca gagaatggag ttgtttttcc ttttatacct
      901 ggcgataaaa tctctaacta ttccatagat ctcttcttta gcctcatcgc taaaggtagt
      961 ataaccagat ttcaatccca ataccataat tgcccctgtt actgccccgc agacctcacc
     1021 tgatctgccg agtcctcctc caaatccgct ggatattaaa agggcgaggt ctttatccag
     1081 cccaagatca ggtccatata ctgatattat ggactgacaa cagttgaatc cgcttttaaa
     1141 tgtctcaatc actgaatcta ctttactcat cagagaacct ccttgataaa tgagttgttt
     1201 ttattataac agagatatat catagttact agaaacaaga gaaatagaat agttttctca
     1261 actgaaagaa aaacttttac gaggaggtta aaaatgttaa atgaaaaaga gtactatgat
     1321 aaagtttatg gttgctggct gggtaaaaat gcaggaggga ctcttggcac cccacttgag
     1381 agtgggtggg gaaaagaaga gatgtttgat gtctggtggt accctaaact gcaggaaggt
     1441 ggactaccca atgatgacct ggagttacaa cttatctggc ttcaggcttt agaagacagg
     1501 ggattagata taactgccag agatttagca gagtactggc tggactgtat agcctataat
     1561 tttgatgagt atggtctgaa taaaacgaat ctcaaaaaag gactcgtacc tccagtatcc
     1621 ggttatttta ataactggtt taaacactgt atggggagtc cgattcgttc tgagatatgg
     1681 gcatgtattg ctccagggtt accaaatgta gctgccagat atgcctacga ggatgcgata
     1741 gtagaccatg ccggtggaga gtctgtatat ggagagatat tcaatgccgt cttagaatcc
     1801 tcagcattct ttatatccga taagataaaa ttacttgaga taggactctc ctttatacct
     1861 gagagctgtg agaccgccaa agctataaga aatgctatga ttgcatatga tagaggaatg
     1921 gactggaagg aggcgaggaa ttttgtgctg aagaactcct atagtccaat agcccaatat
     1981 tctccagtta atcttggttt tcaaaccata ggtcttttat atggagacga ttttggagat
     2041 gccatatgta aggcggtaaa ctgtggctac gatacagact gtacaggagc aacactgggg
     2101 tctatcctgg gtattatact gggtaaagat ggactcccaa gtaaatggat agaaccatta
     2161 agtgataaga tagctacaaa tgcatcctgg ggagggataa agaatgtgag agagccgaaa
     2221 gatctggacg agcttaccag gagagtatgc cgtataggta aaaagataat ggctctacat
     2281 ggagaagagt cggattcatt taaccctgaa gaagagacta agaagttatg gtatatccct
     2341 cctacaaggg tagattatga tttaaagacg gtggttatat ctctggatta tatagataat
     2401 cctgctattc agtttgagaa ggcgaaggtc tttaacctca tattaaagaa tccacatcct
     2461 gagactattg aagcaaagat ttcctgtaga tttccagaag gatgggatat aaatcctgag
     2521 gataagaaga ttataattca accagacgaa gagattacga tcccatatac cgtatcggtt
     2581 cgtgacataa aaattttagg gacatcaaat cgcggaagta tctccatatc tgtaaaggag
     2641 agacctcaga taccagatat tcctgtcgta tatacaggtt caaatagatg gttaatctct
     2701 caaagattcg atggagatat aaataccaag actcctatag aagataatct aaatccaggc
     2761 ggaataagtg atggttggat gataacaaat tttgatgata atgaacttct aattgagcct
     2821 cattttgaag gtaaaccagg tatcgtttac ctgagacact acatatacag tgcctttcca
     2881 agaccggtca ggttagg
//
LOCUS       DAOTKU010000188         2892 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_28118_length_2892_cov_3.660204, whole genome shotgun sequence.
ACCESSION   DAOTKU010000188 DAOTKU010000000
VERSION     DAOTKU010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28118_length_2892_cov_3.660204"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="P5310_05160"
     CDS             <1..235
                     /locus_tag="P5310_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015907501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional 5,10-methylene-tetrahydrofolate
                     dehydrogenase/5,10-methylene-tetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HRU32783.1"
                     /translation="AVGKPRFITKDMVKEGAVVIDVGINRVENKLVGDVDFEGVASIT
                     SLITPVPGGVGPMTVAMLMKNLIAAYNIQSNKK"
     gene            232..786
                     /locus_tag="P5310_05165"
     CDS             232..786
                     /locus_tag="P5310_05165"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HRU32784.1"
                     /translation="MNITKLKIRGKVKRLFDSYPRELLDRWGDIIQDEVLKIVEPYNV
                     IMSYVSLPREVATFKILRKVLEDNKILLVPKVSGEDIISVRIKSLDELSPGTFNVLEP
                     ASNIPYDTSKIEVIIVPGIAFNTKLYRIGHGKGHFDRFLKSLPDNVLKIGLAYEFQII
                     EEEFTDEWDEKMDIIVTEKRRLEE"
     gene            783..2135
                     /gene="lpdA"
                     /locus_tag="P5310_05170"
     CDS             783..2135
                     /gene="lpdA"
                     /locus_tag="P5310_05170"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HRU32785.1"
                     /translation="MNVVIIGGGPGGYVAALRGAQLGLNVTLIEDDKVGGVCLNRGCI
                     PTKALLSASEKLVDIKSSNDFGIEVGDYKIDINKIFNRKDQVVNQLVKGVEYLLSTRG
                     VKLVKGKARLVSQRDVQISTGEIISGDVIIIATGSLPITSIGNLKIDNDRVISSDYAL
                     STPHIPREIVIIGAGAIGVEFATFYKDMGADVSIVEMMSRVVPNEDEEISKTLERVLK
                     RKGIKLYLNNTVKEITEDGVILESGELLRAEKVLVAVGRKPNTDGLGLENAGIKTNNR
                     GYIEVDEYLKTNVPNIYAIGDVTGIALFAHLASHQGIIAIENILGKKKSIDYNAVPRA
                     TFCEPQIGSVGLTEGQARANGINPVIGRFPFRALGYALAIGKWEGMVKLIADESKELI
                     GAHIIGPYASNLLGEVTLAVKEKMRLEDIAETIHAHPTLPEAIMEASFSGLNLPLHIV
                     "
     gene            2145..2552
                     /locus_tag="P5310_05175"
     CDS             2145..2552
                     /locus_tag="P5310_05175"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HRU32786.1"
                     /translation="MRKSRILKSIKDRGLKITPQRQLIAEVLEGNKEHPSAEDVYKKV
                     IEIAPTVSIATVYKTLNSLADLGIIQRLDVGDSKAHFDPDTKSHSHFVCIKCRKIFDV
                     YEELSIPEIDNGFKVLKGQAIYHGLCKDCENSQ"
     gene            complement(2544..>2892)
                     /locus_tag="P5310_05180"
     CDS             complement(2544..>2892)
                     /locus_tag="P5310_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HRU32787.1"
                     /translation="LISDSMSATGLSDGDYELGGLNVTVKSGAPRLPDGRLAGSTLTL
                     GVAVKNMVTRGLIPLPFVSEMASDVPAKSIGETATGSFSYGKKADIVILDKFLRVVRT
                     YIDGRAVYESESL"
BASE COUNT          985 a          475 c          699 g          733 t
ORIGIN      
        1 tgctgtgggt aaacccagat ttatcactaa agatatggta aaagaggggg cagttgtgat
       61 agatgtgggg ataaatagag tagaaaacaa gttggtcgga gatgtagatt ttgaaggagt
      121 ggcaagcata acctctctca taactccggt cccgggtggg gtcggtccaa tgacagtggc
      181 tatgctgatg aagaatctaa tcgccgctta taatatccag agtaataaaa aatgaacata
      241 accaaactaa agataagagg gaaagtaaag aggctctttg attcatatcc aagagagcta
      301 ttagatagat ggggagatat aatacaggat gaggtgctaa agatagtaga accgtataat
      361 gtaattatga gttatgtaag ccttcccaga gaggtagcaa cctttaagat attgagaaag
      421 gttctggaag ataataagat acttttagta ccaaaagtat caggggaaga tataatctct
      481 gtaaggataa agagtctaga tgaactttct ccgggcacct ttaacgttct ggaaccagcc
      541 agtaatatcc cttatgacac atccaaaatt gaggtaatta tagtaccagg aattgctttc
      601 aatacaaaac tttacaggat aggtcacgga aaagggcatt tcgaccgttt tctgaaatct
      661 ctcccggaca acgtattaaa aataggtctt gcctatgaat ttcagataat agaagaggag
      721 ttcaccgatg agtgggatga aaagatggat ataatagtca ctgagaaaag gaggttggaa
      781 gaatgaatgt agtcataatt ggcggaggac caggagggta tgtagctgca ctgagagggg
      841 ctcagctggg gcttaacgta acactgatag aagatgataa agttggaggt gtgtgcctta
      901 atagaggttg tatcccaacc aaggcgcttc tatctgcaag tgaaaagctg gtggatataa
      961 aatcctccaa tgacttcgga atagaggtcg gagattataa aatagatatt aataagatct
     1021 tcaatagaaa ggaccaggtt gtaaatcaac tggtaaaagg ggtagagtat ctgttatcga
     1081 caagaggagt gaagctagta aaagggaagg ctcgactggt aagtcaaaga gatgtgcaga
     1141 tatctacagg agagataatc tctggagatg taattataat agcaaccgga tctcttccaa
     1201 taactagtat agggaatctc aagatagata atgacagggt tatatccagc gattacgcct
     1261 tatccacacc acatataccc agagagattg taataatagg ggcaggagct ataggggtgg
     1321 aatttgccac cttctataag gatatgggag cagatgtaag tatcgtagaa atgatgtcca
     1381 gagttgtccc caatgaggat gaagagatat caaagactct ggaaagggtt ctcaaaagga
     1441 aagggataaa actatacctg aataataccg taaaagagat aacagaagac ggagtaatac
     1501 tggaaagtgg agaattgtta agggctgaaa aggttctggt tgcggtggga agaaaaccaa
     1561 acacagatgg gcttgggctg gaaaatgcag ggataaaaac aaataacaga ggttatatag
     1621 aggttgatga gtatctgaag acaaatgttc caaatatata tgcgatagga gatgtaacag
     1681 gcatagccct atttgcacat ctagcctctc atcagggaat aatagctata gagaatatac
     1741 tgggtaagaa aaagagtatc gattataatg cagtaccaag ggcaaccttc tgtgaaccgc
     1801 agataggaag cgttgggcta actgaaggtc aggcaagggc taacggtata aatcctgtta
     1861 ttggaagatt tccattcagg gcattgggct atgcgttagc cataggaaaa tgggaaggta
     1921 tggtaaagtt aatagctgat gaatctaaag aacttatagg ggcacatata ataggtccat
     1981 atgcatcaaa tcttttagga gaggtaactc tcgctgtaaa agagaagatg cgtctggaag
     2041 atatagcaga gaccattcat gctcatccaa cactaccgga ggctataatg gaggcaagtt
     2101 tttctggttt aaatttacct ctacatatag tataattaga agatatgagg aagagcagga
     2161 ttctcaaaag cataaaagat aggggtctta aaattacccc gcagagacaa cttatagctg
     2221 aggtattgga ggggaataag gaacatccca gtgcagaaga tgtttacaaa aaggtcatag
     2281 agatagcccc cacggtatct atagccaccg tatataagac tttaaacagt ctggcagatc
     2341 tggggattat tcagaggtta gatgtgggag atagcaaggc tcattttgac ccggatacta
     2401 aatctcacag tcactttgtc tgtataaaat gtcgaaagat cttcgacgta tatgaagagc
     2461 tttctatacc agaaatagat aatggattta aggtcttaaa ggggcaggct atctaccatg
     2521 gtttatgtaa ggactgcgaa aactcacaat gattcagact catatacagc tctcccatct
     2581 atataagtcc ttacaactct taagaactta tcaagtataa ctatatcagc ttttttaccg
     2641 taggagaaac tgccagtagc ggtttctcct atactttttg ccgggacatc tgatgccatt
     2701 tctgatacaa atggcagtgg aatcagccct ctggttacca tattctttac tgcaacgcca
     2761 agtgtcaggg tgcttccggc aagtctacca tccggaagtc tgggggcacc actcttaaca
     2821 gtaacattga gccctcccag ttcatagtct ccatctgata gtcctgtagc actcatacta
     2881 tcacttataa gg
//
LOCUS       DAOTKU010000189         2878 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_28316_length_2878_cov_3.318810, whole genome shotgun sequence.
ACCESSION   DAOTKU010000189 DAOTKU010000000
VERSION     DAOTKU010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28316_length_2878_cov_3.318810"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="P5310_05185"
     rRNA            <1..335
                     /locus_tag="P5310_05185"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            383..501
                     /gene="rrf"
                     /locus_tag="P5310_05190"
     rRNA            383..501
                     /gene="rrf"
                     /locus_tag="P5310_05190"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            545..1285
                     /locus_tag="P5310_05195"
     CDS             545..1285
                     /locus_tag="P5310_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012962822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRU32788.1"
                     /translation="MSGHSKWHNIRETKQKMDASRGKVFGKVTRQIIIAVREGGPDPE
                     KNSKLRLALEKAREVNMPSDNIKRAIQRGTGEISGESYEEVIYEGYGPGGVAIMVEAT
                     TDNRNRTTAELRHTFQRNGGSLGESGCVSWIFSRKGYILVSPEEKEDTVYNVALEAEV
                     EDIRNTEDGYEIITSVENFEKVKDNCEKAGLKVIRAETTYLPNNTIKVESDAARQLLR
                     LLEQLEELDDVQKVYGNFDIPDEILINA"
     gene            1278..1784
                     /gene="ruvC"
                     /locus_tag="P5310_05200"
     CDS             1278..1784
                     /gene="ruvC"
                     /locus_tag="P5310_05200"
                     /inference="COORDINATES: protein motif:HMM:NF014166.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HRU32789.1"
                     /translation="MHSDVIIGVDPGSNITGYGILESGGNVFIPLSYGTINVPDLNSR
                     PEDLKCIFKSLSSLIETFNPQVLSLEKVFYYRNVRSSLVLGEVRGIILLLAGLYNIKV
                     MEFTSTQIKSSLTGYGRAGKTQVRFMVKKLLSIQDEIELDASDALALSLCAGLELSRD
                     RCLPILKV"
     gene            1760..2329
                     /gene="ruvA"
                     /locus_tag="P5310_05205"
     CDS             1760..2329
                     /gene="ruvA"
                     /locus_tag="P5310_05205"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HRU32790.1"
                     /translation="MFAYLEGIVSQIKDNFLVLDVKGIGFYLYIPPSLCKKLTVGKTY
                     KIFTSFLLNSEVPALFGFLSEEDRELFELLISISGIGPKCGLALISELGYNGVVRGIK
                     NEDTDTLGGVPGVGKKKAKKLILELKEKLAEIGESSQENVRLLTETLKKLGYNKKDIR
                     EAIEGIDMENNSLEDLIKISLKRLYKKDG"
     gene            2322..>2878
                     /locus_tag="P5310_05210"
     CDS             2322..>2878
                     /locus_tag="P5310_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015907620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRU32791.1"
                     /translation="MDKDIFSPVVLPEERIIESSLRPKSLEDFIGQERVKAQLDIFLR
                     ASIKRGETLDHVLFYGPPGLGKTTLAYIVSSELKVPIQVTSGPAIEKAGELVAILTSL
                     DEGSVLFIDEIHRLGKSVEEILYPAMEEREIDIVIGKGTGARAIRIRLPRFTLIGATT
                     RFGLISPPLRDRFGIVERLDYYSPE"
BASE COUNT          905 a          450 c          725 g          798 t
ORIGIN      
        1 gcggtacgtg agctgggttc agaacgtcgt gagacagttc ggtccctatc cgtcgtgggc
       61 gtaggatact tgagaggagt tactcctagt acgagaggac cggagtgaac agaccactgg
      121 tgtaccagtt gttccgccag gagcagaagc tgggtagcca agtctgggag ggataagcgc
      181 tgaaagcatc taagcgccaa gcccacctca agatgaggta tcccaccggt aaaaccgggt
      241 aaggttccag ggagaatacc tggtagatag gctggaggtg taagggcggt aacgtcttga
      301 gcctaccagt actaatagac cgaggtcttg atattgcccg ctatacagtt ttgaaggtgg
      361 agttaagagt tttaaggaag gactcggtgg taaaagagcg gaggggaagc acccgttccc
      421 atcccgaaca cggcagttaa gccctccagc gccgatggta ctgcagggga gaccctgtgg
      481 gagagtaggc cactgccggg tccgcttttt tgttatctaa actggtttgt attgaggtga
      541 ttttatgtct ggacattcta agtggcacaa tataagagaa accaaacaga agatggatgc
      601 atctaggggt aaggtttttg gcaaagttac ccgtcagatt ataatagcgg taagagaggg
      661 tggacctgat cctgagaaga attctaaact cagattagct ttggaaaagg caagagaagt
      721 taatatgcct tcggataata taaaacgagc tatccagaga ggtaccggag aaatttctgg
      781 agagtcttat gaagaagtta tttatgaggg ttatggtcct ggaggagtag ctattatggt
      841 agaggctact actgataata ggaatagaac taccgctgaa ctcaggcata ctttccagcg
      901 taacggtggt agtttaggag aatccgggtg tgtctcctgg atattttcca gaaaaggtta
      961 tattctggta agtcctgagg agaaagaaga tacagtatac aatgtggctc ttgaagcgga
     1021 agtagaagat ataagaaata ctgaagatgg atatgaaata ataacttctg tggagaattt
     1081 tgaaaaggtt aaagataact gtgaaaaagc aggacttaag gttataaggg cagaaactac
     1141 atatctaccc aataatacga taaaagtaga aagcgatgca gccagacagt tgcttagatt
     1201 attagaacag ttagaggaac tggatgatgt tcaaaaggta tatggtaact ttgatatacc
     1261 tgatgagatc ttaattaatg catagtgatg ttattattgg tgtggatcca ggatccaaca
     1321 taactggcta tggtatatta gagtcaggag ggaatgtctt cattcctcta tcttatggta
     1381 caataaatgt cccggattta aattctcgtc cagaagatct gaagtgtata tttaaaagtt
     1441 tatcttctct tatagagacc tttaatccac aggttttatc tctggagaag gtattctatt
     1501 atagaaatgt aaggagttct ctagtacttg gagaagtccg aggtattata ctccttttag
     1561 caggtttata caatataaaa gttatggaat ttacatcaac tcaaataaag agttctctta
     1621 caggttatgg tagagcaggg aagactcagg tgagatttat ggtaaagaaa cttctctcta
     1681 tccaggatga gatagaatta gatgcaagtg atgccctggc tctttctctg tgtgcgggct
     1741 tggagctctc aagggataga tgtttgccta tcttgaaggt atagtttctc agataaagga
     1801 taattttctc gttttagatg taaaggggat aggattttat ctttatatcc ccccatcact
     1861 gtgtaaaaag ttaactgtag gcaagacata caagatattc acctctttcc ttttaaatag
     1921 tgaggttcct gccctctttg gcttcttatc ggaggaagat agagaactat tcgaattgct
     1981 tatcagtatt tctgggatag gtccaaagtg tggattagca cttataagtg agctcggcta
     2041 taatggtgta gtaagaggaa taaagaatga ggatacagat actctgggag gagttcctgg
     2101 agtagggaag aaaaaagcaa agaaattgat actggagtta aaagaaaaat tagctgaaat
     2161 aggcgaatca tcccaggaaa atgtgagact ccttactgaa accctcaaga aattaggtta
     2221 taacaagaaa gatataagag aggctataga aggcatcgat atggagaata actctctaga
     2281 agatttgatt aagatctctt taaagaggtt atacaagaaa gatggataag gatatcttct
     2341 cccctgtggt gttaccagaa gagagaataa tagaaagttc tcttagacct aagtctctgg
     2401 aggattttat aggacaggag agggtgaaag ctcaattaga tattttttta agggcttcta
     2461 ttaaaagagg cgagacactg gaccatgtat tattctatgg accacctgga cttggtaaga
     2521 ctactctggc atatatagta tccagtgaat taaaagttcc tatccaggtt acttctggac
     2581 ctgctataga gaaggctggg gaattggttg ctatactgac ttctctggac gaaggtagtg
     2641 ttctctttat agatgagata catagacttg gtaaatctgt tgaagagata ctttatcctg
     2701 ctatggagga gagagaaata gatatagtta taggtaaagg gactggtgcc agagctataa
     2761 ggataagact cccaagattt actctgatag gagctacgac caggtttggg cttatatctc
     2821 ctcctttaag agatagattt ggaatagtag aaagattaga ttactattct ccagaaga
//
LOCUS       DAOTKU010000190         2872 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_28414_length_2872_cov_2.389066, whole genome shotgun sequence.
ACCESSION   DAOTKU010000190 DAOTKU010000000
VERSION     DAOTKU010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28414_length_2872_cov_2.389066"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="P5310_05215"
     CDS             <1..361
                     /locus_tag="P5310_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016821430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRU32792.1"
                     /translation="RQFFFSIPYELDEAAYMDGANPLKVLWSIVVPLSKPAIITVAIF
                     NFIGVWNDLLGPIIYLSDPDKYTLAIGLSVFRSMYTAQWHYLMAASVVVLLPVIIVFF
                     IGQRYFIQGITLTGLKG"
     gene            384..2237
                     /locus_tag="P5310_05220"
     CDS             384..2237
                     /locus_tag="P5310_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HRU32793.1"
                     /translation="MSSTFVINNTKSKYCALKSIPVESVKLTDKFWSERIKTMEEVTL
                     PLQYELLEETHRIDNFRRASGKISGDFVGFFFNDSDVYKWIEAVAYLLSYKKNEKLEG
                     LVDSVIEEIRLAQDEDGYLDTYFTFEKKQDRWTNLKDMHELYCAGHLIQAAVAYHRAT
                     GKASLLNIAIKFADHIARIFGPHGREGTPGHPEIEMALVELYRETNDKKYLDLARFFI
                     DERGKGVIGGSSYHIDHKPFRELDEIVGHAVRSLYLNSGATDLYMEIGDETLLDALER
                     LWSNLVEKKMYVTGGAGARKEGEAFGEEYELPNETAYAETCAGIANLMWNFRMLQTLG
                     EARFADIMELCLYNGVLSGISLDGKEYLYVNPLADRGNHRRQRWFACACCPPNIARTI
                     TAIPGYMYSVSSDGIWVHLYASSILKLDINSSSVTVKEETDYPWDGNIEFVLEANSHF
                     KLFLRKPGWCEKFSVSINGEKVNPILENSYIVIEKDWQEGDTVRLNLVMNPELLESHP
                     KVKDNVGRVAIRRGPIIYCVEAEDNPWGDVWDLEISEGTGLVEEYRDILGGLVVVKGE
                     GYVNDPESWNGLYLTRGNARALKREIEFVAIPYYAWANRTPGPMEIWIKRS"
     gene            2534..>2872
                     /locus_tag="P5310_05225"
     CDS             2534..>2872
                     /locus_tag="P5310_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008193671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32794.1"
                     /translation="MKSFRSLLVLILCLGLVLGLVQMSFAQKYPTPSQYSTVADYKKA
                     TGKDIKSFNEAPMLAELVKQGKLPSVDKRLPAEPLVVIPVEEIGKYGGDWRTVTLGVA
                     DNAWFQRTVDY"
BASE COUNT          883 a          434 c          698 g          857 t
ORIGIN      
        1 gaggcagttc tttttttcca tcccatacga gctggatgaa gcagcatata tggatggggc
       61 taatcccctt aaggttcttt ggagtattgt tgttccactg tctaaaccag cgattataac
      121 tgtagctata tttaatttta taggtgtatg gaatgattta ttgggtccga taatatatct
      181 cagtgatccg gataagtata cccttgctat aggtctttct gtttttagaa gtatgtatac
      241 tgctcagtgg cattatctta tggctgcatc tgttgtggtt ttactcccag taataatagt
      301 cttctttata gggcagagat attttattca gggtatcact ttaacaggtt taaagggata
      361 atttaaacga agaggaggat cttatgtcca gtacttttgt tattaacaat actaagagta
      421 agtattgtgc gctaaagtct attcctgtag aatcagtaaa acttacagat aaattctggt
      481 cagaaagaat aaagactatg gaagaggtaa ctcttccctt gcagtatgaa cttctggaag
      541 agacccacag gatagataat tttagaagag catctggtaa gatatctgga gattttgtag
      601 gcttcttttt taatgactct gatgtttata agtggataga agctgtggct tatcttttaa
      661 gttataagaa gaatgagaag ctggaaggtt tagttgatag tgtaatagag gagatcagat
      721 tagctcagga tgaagatggt tatctggaca cctattttac ctttgagaaa aaacaggatc
      781 gctggacaaa cctgaaggat atgcatgaac tctactgtgc tggtcacctg atacaggcag
      841 ctgttgcata ccatagagct acaggaaagg caagtcttct taacatagct ataaaatttg
      901 cagatcatat agcccggatt tttggtcctc atgggagaga aggaacacct ggacatcctg
      961 agatagagat ggctcttgta gaactttaca gagagacaaa cgacaaaaaa tatttagatt
     1021 tagccaggtt tttcatagat gagagaggaa agggtgttat tggaggaagc tcctatcata
     1081 tagatcacaa gccatttaga gaattggatg agattgtagg acatgctgtg aggtctctat
     1141 atctgaactc tggtgctaca gacctctata tggagattgg agatgagact ttacttgatg
     1201 ccctggagag actctggtct aacctggtag agaagaagat gtatgtaaca ggtggagctg
     1261 gagcaaggaa agagggcgaa gcttttggag aagagtatga attaccgaat gagacggctt
     1321 atgcagaaac ctgtgcaggt atagcaaacc ttatgtggaa tttcaggatg cttcagacac
     1381 tgggtgaggc tagatttgct gacataatgg agctctgtct ctataatggg gttctctctg
     1441 gtatatctct tgatggaaaa gaatatctct atgtaaatcc ccttgctgat agaggtaacc
     1501 acagacgaca gagatggttt gcctgtgctt gctgtccacc caatatagcc aggactataa
     1561 ctgctattcc aggttatatg tattcagtct cttctgatgg aatttgggta catctctatg
     1621 ccagcagtat actgaagcta gatattaaca gttccagtgt gactgtaaag gaagagacag
     1681 attatccctg ggatggaaat atagagtttg ttctggaggc aaattctcac tttaagctct
     1741 tcttgagaaa gccgggatgg tgtgaaaaat tctcagtaag tataaatgga gaaaaagtta
     1801 atccaatttt ggaaaatagt tatattgtta tagagaaaga ttggcaagaa ggcgacactg
     1861 taagattaaa tctggtaatg aatccagaac ttttagaaag tcatcctaaa gtaaaggata
     1921 atgtaggacg ggttgcaata agaagaggtc ctatcatcta ctgtgtggaa gcagaagata
     1981 atccctgggg tgatgtctgg gaccttgaga tatcagaagg tacaggtctg gtagaggaat
     2041 acagggatat cctgggtgga cttgttgtag ttaaagggga agggtatgtt aatgatcctg
     2101 agagctggaa tggtctctat cttacaagag gtaatgcaag agcgttaaag agagaaatag
     2161 agtttgtagc gataccatac tatgcctggg cgaatagaac ccctggacct atggagatct
     2221 ggataaaaag atcataaaag atcagtatcc tgttatcagg tatagagaat aaatccccat
     2281 tctggttaga tgtatatata cttggtgtaa aactctaact agaaggggga tttctctttg
     2341 aaagtggtta cacctaattg atttatccca gtcttttaga tataatataa atgctattta
     2401 ttaaaccggg ttttatctgg agttgctaag cctggtggag aggaggtgaa gagaactgca
     2461 tctataattt tgattgggga gtagatgagg ttttcttggt attgaaattt tttatgagga
     2521 ggcttgaaag agtatgaaga gttttagaag tttattagta ttaattttgt gcctgggctt
     2581 agtattggga ttagtccaga tgtcttttgc tcagaaatat ccaaccccat ctcagtacag
     2641 taccgtagca gattacaaaa aggctactgg aaaggatata aaatcattca atgaagcgcc
     2701 tatgcttgct gaacttgtaa aacagggcaa acttccctca gtagataaaa gacttcctgc
     2761 tgaacctctc gttgtcatac cggtggaaga gatagggaaa tacggtggag attggaggac
     2821 agtcacttta ggtgttgctg ataacgcatg gttccagaga acagtcgact at
//
LOCUS       DAOTKU010000191         2870 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_28437_length_2870_cov_3.334281, whole genome shotgun sequence.
ACCESSION   DAOTKU010000191 DAOTKU010000000
VERSION     DAOTKU010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28437_length_2870_cov_3.334281"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="P5310_05230"
     CDS             <1..359
                     /locus_tag="P5310_05230"
                     /inference="COORDINATES: protein motif:HMM:TIGR04085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HRU32795.1"
                     /translation="IPYKAFSIDLKENNILGALCNLGDESMCLMPDGIVYPCRRLPVP
                     AGDLKIDSFDRVLRELKFFKEKINRSILKGKCGHCQLDDCIGCRALAYATSGDIYSED
                     KQCPKDRYGRITLESK"
     gene            381..1391
                     /gene="purM"
                     /locus_tag="P5310_05235"
     CDS             381..1391
                     /gene="purM"
                     /locus_tag="P5310_05235"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HRU32796.1"
                     /translation="MDEIDYKSSGVDIDRGLAFVDIIRKKNLYKPPYILDSIGGFASL
                     VSISSYKDPVLVSSTDGVGTKLLIAQRVNIHSTVGIDLVAMGVNDVLVYGAKPLFFLD
                     YIACGRLNLDVMGDVIQGIVTGCEEAECPLVGGETAEMPDFYPEDRYDLAGFAVGIVE
                     REEIINGKSIKEGDIILGLPSSGLHSNGFSLIRKLIKDRGLSLDGIYPPFNRPLYEEL
                     LIPTRIYWKQLKVLLENRIKIKGIAHITGGGMVDNIPRILPEGVFAYINTENIPHKPI
                     FTFIDKMLKLPREEMYRTFNMGIGMILIVEPEDREKAFGLLPELIELGEIRSGKRGVT
                     IG"
     gene            1384..2010
                     /gene="purN"
                     /locus_tag="P5310_05240"
     CDS             1384..2010
                     /gene="purN"
                     /locus_tag="P5310_05240"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HRU32797.1"
                     /translation="MDKKLKIGVLASGRGTNLQAMIDGIQRGELSAEIAIVISDNRDA
                     MALERARKANIPTVYVKPGKKSEFEREVINILEENNAELIVLAGFMRILSPNFVNHFP
                     LKIINIHPSLLPSFPGLEAQRQAIEYGVKITGCTVHFVNEMVDAGPIILQEAVPVYDD
                     DTVESLSDRILEKEHKILVEAVRIFSENKLKVIGRRVFIDKTGSDKCL"
     gene            1979..>2870
                     /locus_tag="P5310_05245"
     CDS             1979..>2870
                     /locus_tag="P5310_05245"
                     /inference="COORDINATES: protein motif:HMM:NF013931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HRU32798.1"
                     /translation="MIKQALISVYDKKYIVDFARFLSSKGIKLISTSGTAKLLRDNGL
                     EITDVSAITGFREILGGRVKTLHPSILAGILARREDQLEELYSLGIEPIDIVVVNLYP
                     FEGVVKRGAELEEALENIDIGGVTLIRASAKNFKDVVIITDPEDYGTVEKEIESTGDV
                     SYEARKSLAIKAFAVTSRYDSIISNYLDGSRTPKYFLVGGERSLELRYGENPHQIASA
                     YTTSGISVLSAKQIQGKELSYNNILDLDIAWSLSRSFEKPCVSVIKHNAPCGVAVAED
                     ILSAFNNAWQADPVSAFGSIL"
BASE COUNT          891 a          457 c          717 g          805 t
ORIGIN      
        1 ttattccata caaggcattt tctatagacc tgaaagagaa taatatactt ggagctctct
       61 gtaacctggg tgatgaatct atgtgtctta tgcctgacgg aatagtgtat ccctgtagaa
      121 gattacctgt ccctgcagga gacttaaaga tagatagctt tgacagggtt cttagagaac
      181 tcaagttttt caaagagaag ataaacaggt ccatactcaa ggggaaatgt ggacactgtc
      241 agttagatga ttgtatagga tgtagagcac tggcttatgc taccagtggt gatatttatt
      301 cggaagacaa gcaatgtcct aaagacaggt atggtagaat aaccctggaa tcaaaataaa
      361 aataaattgg agtgaaaggt atggatgaga tagattacaa gtcttcgggg gtagatatag
      421 acagaggatt ggcttttgta gatataatca gaaagaaaaa tctctataaa cccccttata
      481 tactggatag tataggtggt tttgcatctc tggttagtat ttctagttat aaagaccctg
      541 tcctggtatc atccaccgat ggtgttggta caaagcttct tatcgctcag agggtcaata
      601 tacacagcac ggttggtata gaccttgtcg ctatgggtgt taatgatgta ctggtatatg
      661 gggcaaaacc tttattcttt ctggattata tagcctgtgg cagattaaac ctggatgtta
      721 tgggggatgt tatccagggt atcgtgaccg gttgtgagga ggcagaatgt cctcttgtag
      781 gcggagaaac ggcagagatg ccagacttct acccagaaga taggtatgac ctggcaggtt
      841 ttgctgtcgg tatagttgag agggaagaga taattaatgg aaagagtata aaagagggag
      901 atatcatact ggggttacca tcatctggac tccacagtaa tggcttttct ctcataagaa
      961 agctcattaa ggacagaggt ctttctctgg atggaatata tcccccattt aatagacctc
     1021 tctatgaaga actgctcata cccacaagga tatactggaa acagctgaag gttcttctgg
     1081 aaaataggat aaaaattaaa ggtatagcac acataaccgg tggggggatg gtagataata
     1141 taccgaggat acttcctgaa ggagtgtttg catatatcaa tactgaaaat attccacata
     1201 aaccaatttt tacctttatt gataagatgt taaaacttcc gagagaagag atgtacagga
     1261 catttaatat gggaataggg atgatcctta tagtagaacc tgaagataga gagaaggctt
     1321 ttgggcttct tccagagctg attgaactgg gagagataag gtctggaaag aggggtgtga
     1381 caattggata aaaagctgaa gataggtgtt ttagcttcag ggagaggaac gaatctgcag
     1441 gcaatgatag atggtattca aaggggagag ttatctgcag agatagctat agtgataagc
     1501 gataaccggg atgctatggc tcttgagagg gctagaaagg ctaatatccc aactgtctat
     1561 gtgaaaccag ggaagaagag tgagttcgag cgcgaggtca taaatatact ggaagaaaat
     1621 aatgcggagt tgatagtcct ggctgggttt atgagaatct tatccccaaa ttttgtcaat
     1681 catttccccc tgaagataat aaatattcat ccatctttac ttcccagttt ccctggactt
     1741 gaagctcaga gacaggcgat cgaatatggg gtgaaaataa ctggatgcac agttcatttt
     1801 gtgaacgaga tggtcgatgc cggtccaatc attctgcagg aagctgtccc tgtatatgat
     1861 gatgatacag ttgaaagcct atctgacagg atattggaga aagaacataa gatactggtt
     1921 gaagctgtaa ggatttttag tgaaaataaa ttgaaggtaa taggaaggag ggtctttatt
     1981 gataaaacag gctctgataa gtgtttatga taagaaatat atagttgatt ttgccagatt
     2041 tttatcttca aaggggataa aacttatctc cacgtcaggt actgcaaagc ttttgaggga
     2101 taacggtttg gaaattacag atgtatctgc aataaccggt ttcagagaaa ttcttggagg
     2161 cagggttaag actcttcatc cctctatact tgctggtata cttgccagga gagaagacca
     2221 gctggaagag ttatactctc tgggtataga acctattgac atagttgtgg tgaacctcta
     2281 tccctttgaa ggggtggtaa agagaggggc agagttagag gaggctttag agaatattga
     2341 tataggtgga gtaacactga taagagcatc agccaagaat tttaaggatg tggtaattat
     2401 tactgatccg gaggattacg ggactgtaga aaaagagatt gaaagtactg gtgatgtatc
     2461 ttatgaagcc agaaaatctc ttgccataaa ggcatttgca gtaacttcaa gatatgattc
     2521 tataatatct aactatcttg atggctccag gactccgaag tactttctgg tcggtggaga
     2581 aaggtctctg gaactgaggt atggagaaaa tcctcatcag attgcttctg cgtataccac
     2641 atctggtatc agtgtgcttt ctgcaaaaca gatccagggt aaggagctat cctacaataa
     2701 catactggac ctggatatag cctggtctct atccagatct tttgaaaaac cctgtgtgag
     2761 tgtgataaaa cataatgccc cctgtggggt ggcagtagca gaggatatct tatcggcatt
     2821 taataatgca tggcaggctg acccggtttc tgcctttggc agcatactgg
//
LOCUS       DAOTKU010000192         2868 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_28472_length_2868_cov_2.823676, whole genome shotgun sequence.
ACCESSION   DAOTKU010000192 DAOTKU010000000
VERSION     DAOTKU010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28472_length_2868_cov_2.823676"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="P5310_05250"
     CDS             complement(<1..477)
                     /locus_tag="P5310_05250"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HRU32799.1"
                     /translation="MSFLKVDGPYIKDETGKKIFLRGVCFGGWLNMENFITGYPGAES
                     SIRQSIKEELGEERYQVFFSSLLDSFITEGDFKFLKEIGATAVRIPFNYRHFEDDMNP
                     GNYSSSGFYYLDRAIEWAKKYNIYIILDLHAAPGWQNQGWHSDNPYGISLFWNDRHY"
     gene            complement(496..1476)
                     /locus_tag="P5310_05255"
     CDS             complement(496..1476)
                     /locus_tag="P5310_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRU32800.1"
                     /translation="MGQEIKLLDIKNLVKVFTIGSGFVKSRITAVDNVSFDLESGRSE
                     IFTLAGESGSGKTTLARIILGFIEPTSGKILYKGKNITEIKGRKEEMWFMREVQPIFQ
                     NPFETFNPLKKVDSYLYETVVNYGIANKREDINSAIDEALNLVGISFQEIQGRYPHEF
                     SGGQLQRISIARALITKPSLLIADEPVSMVDASLKMSICNLFKDLKEKQKVNVIYITH
                     DLATSYYVSDRIAIMFRGTIVEIGPVEKILIDPKHPYTKILRDSVPEPDPEKRWKEKI
                     KLSSFEEREFLSIGCKFADRCPYVMDICKKEAPNDIILDGVTVKCHLYSA"
     gene            complement(1460..2461)
                     /locus_tag="P5310_05260"
     CDS             complement(1460..2461)
                     /locus_tag="P5310_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRU32801.1"
                     /translation="MSVLKVKDLKSYYISEVFGIKRTVRAVDGVYMEINRDEVFGIAG
                     ESGCGKSTLIKTLLGMIKPPLTIVDGEIYYDFGDKKFNILSLNPDESRKIRWEYISYI
                     PQSSMSVLNPVRRIRDTFKSFLGAHKKFEKDELEGLVKEHLSALGLPTEVLNAYPHQL
                     SGGMRQRVTIALASILKPKVILADEPSTALDVVVQRGVIQLLRNIQEEQKNIIIIVTH
                     DMGIHANICDRIAIMYAGKIIEEARTDEIFKHPLHPYTTYLIGSLPKIGDKSYRVSAP
                     GSPPPLSNLPNGCSFHPRCPYVMEKCKKEFPSLLNVEDEHRVACFLVNKEDDNGARD"
     gene            complement(2466..>2868)
                     /locus_tag="P5310_05265"
     CDS             complement(2466..>2868)
                     /locus_tag="P5310_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRU32802.1"
                     /translation="RVIRSQILSLREREFTYTARLSGMKTSSIILKEYLPFIVPLIMA
                     TAMNNMLWAIGMEVTLAILGLSSLEIPTLGTMIHWSVNSQSLLLGIWWWILTPVIICI
                     FLFISLYMLSVSISEFLDPRSRLKRIEVMKE"
BASE COUNT          817 a          608 c          476 g          967 t
ORIGIN      
        1 ataatgtcta tcattccaga acagtgatat tccgtaagga ttatcagagt gccacccctg
       61 gttttgccat cctggtgcag catgaaggtc aaggatgata tatatgttat acttttttgc
      121 ccactcaata gcccggtcaa ggtagtaaaa tccagaagaa ctgtaattgc ctggattcat
      181 atcatcttcg aaatgcctgt agttaaatgg aattcttact gcggtggctc ctatctcttt
      241 aagaaattta aagtcaccct cagttataaa tgagtctaac aggctagaga agaatacctg
      301 atatctttct tccccaagct cttcctttat agactgtcta atagaacttt ctgcaccagg
      361 atatccggtg ataaagttct ccatatttaa ccatccgcca aaacatactc ccctcaggaa
      421 tatcttctta ccggtctcgt ccttaatata aggaccatct acttttaaaa aactcataaa
      481 ttcatctcct tcctattaag ctgagtaaag atgacattta acagtaactc cgtccaatat
      541 gatgtcattg ggtgcttctt tcttacatat atccatcaca taagggcatc tatcagcaaa
      601 cttgcaacct atacttaaaa attctctctc ttcaaatgat gagagcttta ttttttcttt
      661 ccatctcttc tccgggtcag gttctggaac agaatctctt aagattttgg tatatggatg
      721 ttttggatct ataagaatct tttcaacagg acctatctca actatggtac ctctgaacat
      781 tatggctatt ctatcactga cataatagga ggtagcgagg tcatgagtta tatatattac
      841 attgaccttc tgcttttctt tgaggtcctt gaatagattg catattgaca tcttcagcga
      901 ggcgtccacc atcgaaacgg gctcgtcggc aattaagaga gatggtttgg tgataagcgc
      961 tctggcaata gatatccttt gaagttgtcc accagaaaat tcatgaggat atctcccttg
     1021 aatctcctgg aatgatatac caactaaatt gagtgcctca tctattgcgc tgttgatatc
     1081 ttctcttttg ttggcaattc catagttaac tacagtttca tagagataag aatcgacttt
     1141 ttttagggga ttaaatgtct caaatgggtt ttggaatata ggttgaacct ctctcataaa
     1201 ccacatctct tcctttctcc cctttatttc tgtgatattt tttcctttgt ataggatttt
     1261 acccgaggta ggttcaataa aacccaatat tatccttgcc aatgttgttt taccgcttcc
     1321 actctcccct gccagagtaa atatctctga tctaccagat tcaagatcaa atgatacgtt
     1381 atctactgca gttattctgc ttttgacaaa tccgctacct attgtaaata cctttaccag
     1441 attctttata tccagaagtt taatctcttg ccccattatc atcctccttg ttaacgagaa
     1501 aacatgctac tctgtgttcg tcttcaacat ttagcaaaga tggaaactct tttttacatt
     1561 tctccattac ataagggcat ctaggatgaa aactgcatcc atttggaaga ttactaagag
     1621 gtggaggaga gcccggggca ctaactctat aacttttatc tcctattttt gggagagaac
     1681 ctatcagata ggtcgtatag ggatgtaatg gatgcttgaa tatctcatcg gttcttgcct
     1741 cctctattat tttacctgca tacatgatgg ctatcctgtc acatatattg gcatgaatac
     1801 ccatgtcatg ggtaactatt atgatgatat ttttttgttc ctcctgaata ttcctgagaa
     1861 gctgtataac gcctctttgc acaactacat caagggcagt ggaaggttca tcagcaagaa
     1921 tgactttggg tttgagtata gatgcaaggg caatagttac tctttgtctc attccacctg
     1981 aaagctggtg gggatatgca tttaatactt ctgttggtag tccgagtgca ctgaggtgtt
     2041 cctttaccag accttcaagt tcatcttttt caaatttttt atgggcaccc aggaaacttt
     2101 tgaaggtatc tctgattctt ctaacaggat taagaacact catagaactt tgaggtatat
     2161 aagaaatata ttcccatctt atttttctcg attcatctgg atttaaggag agtatattga
     2221 atttcttatc tccaaaatca tagtatatct ctccatctac aattgttaga ggtggtttta
     2281 tcatcccaag aagagttttt ataagagtac ttttcccgca tccagattcc cctgcaattc
     2341 caaagacctc atctctatta atctccatat ataccccatc caccgctctg acagtccttt
     2401 taataccaaa tacctctgag atataatacg attttaaatc tttgacttta agtactgaca
     2461 tatcattact ccttcataac ctcaatcctc tttaatctgc tcctaggatc gaggaattcg
     2521 cttatactta ctgagagcat atatagagat atgaataaaa atatacagat tataactggt
     2581 gttaatatcc accaccatat accaagcaac aatgattgcg aattaaccga ccagtgtatc
     2641 attgttccca gtgttggaat ttcaagactt gataatccaa gtatagctaa tgttacctcc
     2701 ataccgatag cccagagcat attattcata gcggtagcca taattaacgg cacaataaat
     2761 ggaagatatt cttttagaat tatactggat gtcttcatcc cggataatct tgcggtatat
     2821 gtaaactccc tctctctaag gcttaggatt tgcgacctaa tgaccctc
//
LOCUS       DAOTKU010000193         2865 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_28501_length_2865_cov_3.460498, whole genome shotgun sequence.
ACCESSION   DAOTKU010000193 DAOTKU010000000
VERSION     DAOTKU010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28501_length_2865_cov_3.460498"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1163)
                     /locus_tag="P5310_05270"
     CDS             complement(<1..1163)
                     /locus_tag="P5310_05270"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRU32803.1"
                     /translation="MKRVLRLSNYLFKSLVRDGSNLFWLLAFPCILLVIMMLIFSPLY
                     EPKNITFNLTLISYGGTFSEIIERVFNEVSSGENRIFQLEKFPGSGENLEKGLERLKD
                     KKTDLVLEIPEDFDARMLNWFMMKNMGITPPAPTINVYSLKHQAASQSAYVIVKNIFQ
                     KLELEFLKGMGYQMEIIPVKTEIVGTEDTFSYIDFIYPGMVIFTVFMTGLFGIGVDMT
                     WLKEKGIMRRINVTGLANSEFIISYIISRFYLIILQIALITIIAKFIFKTNVNPFAPA
                     FLLYTLLTILCLSSLGFFIAAISKTTNSANAISQVLNFPLQFLGGLYFPLINVPWVIR
                     WIVIINPITYLTSGIRDSLGIMKSPYPDIVTLLVPVFWTVFFLFFTVKRFGKE"
     gene            complement(1160..2086)
                     /locus_tag="P5310_05275"
     CDS             complement(1160..2086)
                     /locus_tag="P5310_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012582519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRU32804.1"
                     /translation="MSNFILEVEDLKRYYGQVKAIDGVNFKVKQGSVFTLLGPNGAGK
                     TTTIEIIEGLRIPDSGKIRFLGKEVSKIGREEKAYIGVSLQETNLIPYLTVRETLDMF
                     KNIFERSLDADEVLKMVSLEDKSSTYIKNLSGGQKQRLVIGLAMINDPLILFLDEPTT
                     GLDPQARRNTWDILLDLKRNGKTIILTTHYMEEAEILSDWVCIMDLGKIIKEGDPHSL
                     IRDLGKESLIEVELDVNSVMLEELNNSGLDPVYDERHKRLLLKTNDILSTIDLLLRLS
                     RENALQIKDVTIRQPNLEDVFLSLTGKSLREE"
     gene            complement(2086..2550)
                     /locus_tag="P5310_05280"
     CDS             complement(2086..2550)
                     /locus_tag="P5310_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012582556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HRU32805.1"
                     /translation="MLLEKINRDYIEAKKKRDTLRESVLNLLKSNIKYKEIDAKGKGK
                     TLEDNDIIDIIRQEIKKRRESIELYKQGGRLELAEKEAEELKILQGYLPEELSEDEIR
                     VIINRIVESTGASSLKDLGNVMKETMKELRGKAEGGLIRKIVEDVLREKEET"
     gene            complement(2590..>2865)
                     /locus_tag="P5310_05285"
     CDS             complement(2590..>2865)
                     /locus_tag="P5310_05285"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HRU32806.1"
                     /translation="EADRRFHQILREITEDKRLSYFLSILDAQLKLALPVINSLSGRV
                     ESSLQEHKKILSSLEKKNIQNAEFYSKEHMRLEREAVIRELKFRGFK"
BASE COUNT          858 a          640 c          439 g          928 t
ORIGIN      
        1 tcctccttac caaatctttt tacagtaaaa aacaggaaga acactgtcca gaatactggc
       61 acaagtaaag ttacaatatc aggatatggg gatttcatta tgcccagaga atctctgata
      121 ccagatgtta aataagtgat cgggttgata atcacaatcc atcttataac ccatgggaca
      181 ttaataagag gaaaatataa tcccccaaga aactgtaatg ggaaatttaa cacttgactt
      241 atagcattgg cactgttagt ggtctttgaa atagcagcta taaaaaatcc aagagaggat
      301 agacacagta ttgtaaggag cgtatatagt agaaatgccg gagcaaatgg gttgacatta
      361 gtcttaaata taaattttgc gatgatagtt ataagtgcta tctgcaggat gataagataa
      421 aacctggaga taatataaga gataataaat tcagagtttg ccagaccagt aacatttatc
      481 ctgcgcataa tacccttctc ttttagccag gtcatatcta cgccaatacc aaagagacct
      541 gtcataaaaa cggtaaaaat aaccatcccg ggataaataa aatctatata tgaaaaagta
      601 tcttcagttc cgacaatctc agtttttaca ggtattatct ccatctgata ccccatgcct
      661 ttaagaaact caagctccag cttctggaat atattcttca ctatgacgta tgccgactgg
      721 gaggctgcct gatgttttag agaatacaca ttgatagtag gagcaggagg agttataccc
      781 atatttttca tcataaacca gttcagcatc ctggcatcaa aatcttcagg gatctccaat
      841 acaaggtccg tctttttatc ctttaacctc tccagtcctt tttccagatt ctctcctgaa
      901 cctggaaatt tttcaagctg gaagatccta ttctctccac tgctgacctc attgaagacc
      961 ctctcaataa tctctgagaa tgtccctcca taagagatca gggtaagatt aaatgttata
     1021 tttttgggct cataaagtgg agaaaatata agcatcataa ttacaagtaa tatacaggga
     1081 aaggctaaaa gccagaataa gttagaacca tctcttacca gagatttaaa tagataattg
     1141 gatagtctta agaccctctt cattcttctc tcagtgactt tccagtaaga gataaaaaga
     1201 catcctccag atttggctgt cttattgtaa catcctttat ctggagcgca ttctccctgg
     1261 aaagtcttaa caacaggtca atggtagaga gtatgtcatt cgtttttaac aaaagtctct
     1321 tatgtctctc atcatacaca gggtctaatc cagaattatt taactcctct aacattactg
     1381 aattaacatc aagctctacc tcaataagac tctccttacc cagatctcta ataagagagt
     1441 gagggtctcc ctctttaatt atcttaccca ggtccattat acagacccag tcagaaagga
     1501 tctcagcctc ttccatataa tgagtggtga gaataatcgt cttaccattt ctcttaaggt
     1561 caagaagtat atcccaggta tttctccttg cctgagggtc aagaccagtg gtaggctcat
     1621 ccaaaaataa tatcaatggg tcattaatca tggctaatcc gataacaagt ctctgtttct
     1681 gacctccgga aagatttttt atatatgtac tggacttatc ctccaaagat accatcttca
     1741 ggacttcatc cgcatcgagc gacctttcaa atatgttctt aaacatatcc agggtctctc
     1801 tgacagtaag gtacgggata agattggtct cctgcaagga aacaccaata taagccttct
     1861 cttctctccc tatttttgat acctctttgc caagaaatct gatcttacca ctatcaggta
     1921 tcctgagccc ttctattatt tcaatagttg tagtcttccc cgccccattg ggacctaaaa
     1981 gagtaaatac tgaaccctgc tttactttaa aattaacacc atcaatcgcc ttaacctgcc
     2041 catagtatct ctttaggtct tcaacttcaa gtataaaatt actcattagg tttcctcttt
     2101 ctcccttaaa acatcctcca ctatcttcct tataagtcca ccttctgctt tccctctgag
     2161 ctctttcata gtctccttca taacattacc taaatccttc aaactgctgg ctcctgtact
     2221 ttcgacaatc ctgtttataa tgaccctgat ttcatcttct gaaagctcct ctggaagata
     2281 accttgaagt atctttaact cctcagcctc tttctctgcc agctctaatc tgccaccctg
     2341 tttatagagc tctatggatt ctctcctctt ctttatctcc tgtcttatta tatctataat
     2401 atcgttatct tctaatgtct tacctttacc cttagcatct atctctttat acttgatatt
     2461 agacttgaga agattcaata cactctctct caatgtatcc cttttctttt ttgcctcaat
     2521 ataatctctg tttatctttt ctaagagcat acgcccctcc ttaattacag aattatatta
     2581 aatttttatc tatttaaatc ctctgaactt gagttctctt ataacagctt ctctctccaa
     2641 tctcatatgc tctttggagt aaaactcggc attttggata tttttcttct caagagaaga
     2701 aagtatcttc ttatgttcct gaagggatga ctcaactctt ccagatagag aatttataac
     2761 gggtaaagcc agtttgagtt gagcgtccag tatggaaagg aaatagctga gtcttttatc
     2821 ctctgtaatc tctctcaaga tttgatgaaa tctcctatct gcctc
//
LOCUS       DAOTKU010000194         2849 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_28703_length_2849_cov_3.351467, whole genome shotgun sequence.
ACCESSION   DAOTKU010000194 DAOTKU010000000
VERSION     DAOTKU010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28703_length_2849_cov_3.351467"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="P5310_05290"
     CDS             <1..425
                     /locus_tag="P5310_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HRU32807.1"
                     /translation="ITGRDYISLRNESLSILSREAELREITRLVGVEAISIKERFLLE
                     IARSIREDFLLQNAFNDLDTYTSLDKQLHILSVILEFYHMGLSALDSGVSLEDILNLP
                     VREAISRMKYIPEQEKMRFIEIEDRIKEEFSKLTGARV"
     gene            427..1770
                     /locus_tag="P5310_05295"
     CDS             427..1770
                     /locus_tag="P5310_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HRU32808.1"
                     /translation="MIKEYFSIREIAGPLMLVEGVEGAKYDEMVEVELPDGDVRRGRV
                     LEINEDKALVQLFEGATGLELARSKVRFTGRGIELGVSLDMLGRIMSGFGRPIDGTPP
                     YIPEARLDINGNPINPFNRDYPSEFIQTGISSIDLMNPLVRGQKLPIFSASGLPHNRV
                     AAQIARQAKILTAEESFAIVFAAMGITFEEAEFFIDDFRRTGALETAIERIATPRLAL
                     TAAEYLAFEKDMHVLVLLTDMTNYCEALREVSAARKEVPGRRGYPGYLYTDLASMYER
                     AGRIRGKKGSITQMPILTMPEDDITHPIPDLTGYITEGQIILDRSLHRRGIYPPVNVL
                     PSLSRLKDKGIGAGKTRDDHADISNQLFAAYARGEEVKELAVILGEGALSDVDRRYIE
                     FANRFENELVKQGEYEDRSIEDSLNIAWDLVRILPREELKRIRDKYLEEYLDARD"
     gene            1757..2377
                     /locus_tag="P5310_05300"
     CDS             1757..2377
                     /locus_tag="P5310_05300"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016471 - vacuolar proton-transporting
                     V-type ATPase complex [Evidence IEA];
                     GO_function: GO:0008553 - P-type proton-exporting
                     transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HRU32809.1"
                     /translation="MPEIRVRPNRMELLKLNRRLNTARRGHKLLKDKQEELMRQFLNR
                     IRELESLLSELIVSLEDVNRAFQLASALGDPFLFDRSLVEPLSRMEIDVKWSSVVNVP
                     VPGIDVTYPGPLLYGFLGVSMELDSAYRQLMDSLDKLIHLIELEESVIELGLSLESTR
                     RRVNALEYELIPQLESAIRFISMKLEEMQLSTTTRLMRIKDIIRGR"
     gene            2454..2621
                     /gene="rpsU"
                     /locus_tag="P5310_05305"
     CDS             2454..2621
                     /gene="rpsU"
                     /locus_tag="P5310_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011244628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HRU32810.1"
                     /translation="MPEVQVQENESIESALKRFRREIQKSGILSEIRKHEHYEKPSEK
                     KKREASRKRKR"
     gene            2669..>2849
                     /locus_tag="P5310_05310"
     CDS             2669..>2849
                     /locus_tag="P5310_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32811.1"
                     /translation="MQGGGGDPRARVTHKGVLGIISGQIKGKSVASADIFGKGGFATD
                     IDAILRKMKPKTRYNI"
BASE COUNT          917 a          467 c          739 g          726 t
ORIGIN      
        1 gcattaccgg tagagattat atatctttaa gaaatgaatc cctgagtata ctgagtagag
       61 aggcggaact cagagagatt acaagattgg taggggtaga ggcgatctct ataaaggaga
      121 gattcctgct tgagattgct cgatctataa gggaggattt tctcctgcaa aatgccttta
      181 atgatctgga tacatacact tcactggata agcaacttca tattttgagt gtaattctgg
      241 aattctatca tatgggatta tcagctttag atagtggggt aagtcttgag gatatactta
      301 acctgcctgt tagagaagct atctcccgta tgaaatatat acctgaacag gaaaagatga
      361 gatttattga aattgaagac aggataaagg aagaattttc taaactgacg ggagcaagag
      421 tctgaaatga taaaggaata ttttagtatc agagaaatag caggacctct tatgctggta
      481 gagggggtag agggggctaa atatgatgag atggtggagg tagaacttcc tgatggtgat
      541 gtcaggagag gaagagtatt ggagataaat gaggataagg ctctggttca attatttgaa
      601 ggggctaccg gattagagct agccaggagt aaagtaagat ttaccgggag agggatagag
      661 ttaggagtct ctcttgatat gcttgggaga ataatgagtg gatttggtag acccatagat
      721 ggtactccac catatatacc agaggcaaga ttagatataa atgggaaccc tataaatcca
      781 ttcaacagag attatccttc agagtttatc caaacaggaa tatcgtccat agatttgatg
      841 aatccattgg taaggggaca gaagcttcca atattctcgg cttctggtct tcctcataat
      901 agagtggcag cacagatagc ccgtcaagcc aaaatattaa ccgctgagga aagcttcgct
      961 atagtctttg cggcaatggg tataacattt gaagaggcgg aattctttat agatgatttc
     1021 agacgtaccg gagctctgga gacggctatt gagcgtatag ccaccccaag attggctctt
     1081 acagctgcag aatatctggc attcgaaaaa gatatgcatg tcctggtatt gcttacagat
     1141 atgacgaatt actgtgaggc attgagagaa gtatcggcag caagaaaaga ggtccccggg
     1201 agaaggggat accccggata tctgtatacc gacttggcaa gtatgtatga aagggctggg
     1261 aggataaggg gtaagaaggg ttctattacc cagatgccca tacttacaat gccagaagat
     1321 gatataacac atccaatacc ggacttaacc ggatatataa cagaggggca gataatcctg
     1381 gacaggtcct tacatagaag agggatatat ccgccggtaa atgttttacc atctctttcc
     1441 aggttgaagg ataaaggaat aggtgctggc aagacgagag atgaccatgc agatatctct
     1501 aaccagttat ttgcagcgta tgccagggga gaggaggtaa aagaacttgc ggtgatactt
     1561 ggagagggag ctcttagcga tgtggataga aggtatatag aatttgccaa tcgttttgaa
     1621 aatgagcttg ttaaacaggg agaatatgag gataggtcta tagaagactc attaaatatc
     1681 gcttgggacc ttgtcaggat acttcccaga gaagagttaa aacgtataag agataaatat
     1741 ctggaggagt acttagatgc cagagattag ggttcgtccc aacaggatgg aactcctcaa
     1801 gctgaataga agactcaata ccgcaaggag agggcataaa ctgttaaaag ataaacagga
     1861 agagctaatg aggcaattcc tcaaccgtat aagggaactt gagtcccttc tgtcagaact
     1921 tatagtatct ctggaagatg ttaatagggc ttttcaactg gcaagtgccc ttggagaccc
     1981 gttcctcttt gacaggtctc ttgtagaacc gcttagcagg atggagatag atgtaaaatg
     2041 gagctctgtt gtaaatgtcc ctgtaccagg catagatgtg acatatccag gaccgctact
     2101 ttatggcttc ctgggggtat ctatggagct ggattctgcg tatagacagc tgatggattc
     2161 tctcgataag cttatacatc ttatcgagct ggaggagtcg gtaatagaac ttggtctctc
     2221 tctggagagt accaggagaa gggtcaatgc attggagtat gaacttatac ctcagcttga
     2281 atcggctata aggtttatat ctatgaaact cgaggagatg cagttgagca ccactacaag
     2341 gcttatgagg ataaaggata ttattagggg taggtaagac tattgaaaaa tttgcataag
     2401 aagtttataa ttcaattgat taaagtttaa attgaaagga ggggctaaat gtaatgcctg
     2461 aagtccaggt tcaagaaaat gaatcaatag aaagtgcatt aaagaggttt agaagggaga
     2521 ttcaaaaatc aggtatactc tctgagattc gtaagcatga gcattacgaa aaacctagcg
     2581 aaaaaaagaa gagagaggct tccagaaaga ggaagagata aagactgaca aaaagaagct
     2641 caaacccaag aatcaaggtg ccggtggtat gcagggtggt ggaggagatc ctcgtgcccg
     2701 tgttacccac aaaggtgtgc ttggtattat ctccggacag atcaagggta aatctgtcgc
     2761 gtccgcggat atcttcggta aaggtgggtt cgccactgat atcgatgcga ttctgcgaaa
     2821 aatgaaaccc aaaacgcggt acaacattc
//
LOCUS       DAOTKU010000195         2845 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_28735_length_2845_cov_4.116846, whole genome shotgun sequence.
ACCESSION   DAOTKU010000195 DAOTKU010000000
VERSION     DAOTKU010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2845
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28735_length_2845_cov_4.116846"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1893
                     /locus_tag="P5310_05315"
     CDS             <1..1893
                     /locus_tag="P5310_05315"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HRU32812.1"
                     /translation="IYVALLIVMFVTMSGTFAISSDKIKISGIEVQGNSRISKDEIIK
                     LLELPDEVDEEILKAGLQKLIDTGYFYNVDTSIKVVDNKYILVLNVVELPAFKGVEVT
                     GNREISTDEIKNLISLKPGDAINLTILRSDLEKIANLYKERGYVGANFQFSMTADGLV
                     TVNINEGPVVSDFEFKGNEVLKSEELGKALDKYKGRTLSINLLKEMVTCIQELYNKNG
                     YPACVILNAYSNDSGVVTFEIGEGKISKIEIKGNNKTKDYVILREMETKVGSVLNADK
                     LQKDLQKIFNLGYFSDIKADIHASETPGEIILTIEVVEQLTGQANGGLAYSTRDGLSL
                     LLGISDSNLLGTGRKLGLSLNIGLTARDISLNYTEPYIFGDLSTLDCLLISRQAENSD
                     IIGTSTVKYNEDRKTLDLTLSRPIDSNLRGTVGVSCNDIIYSPDEDGTTLPDILQSGL
                     SNAILLGVARDTRDVVFNPTKGDYYGTSIKFGGGILGGDFSFTKLTLDMRWYKPVTDK
                     ETVAINYTWNFGSGNLPYIEKSSVGGVNSVRGLPENWKKSDNTEILSFEYRIKIQENV
                     SGVVFLDFGNGWDSDETINPTDLYTGIGLGLRIPLPPMGILRLDYGWGSYDWQGRFYF
                     SIGHKF"
     gene            1911..2378
                     /locus_tag="P5310_05320"
     CDS             1911..2378
                     /locus_tag="P5310_05320"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HRU32813.1"
                     /translation="MKNKILIISVLVVGIAGIVLWGLGIPRYGFALDTFKVGYIDYNK
                     VIESHPRMEKAKKDLSDFAQKTQTDYQKQLDEAVKNKTANEAQQIKAEYESRLNETVN
                     KRWQELLNPIINDVKKAVSDVAKKNGIDVVLRGDIVITGGIDITDDVIKVIKK"
     gene            2375..>2845
                     /locus_tag="P5310_05325"
     CDS             2375..>2845
                     /locus_tag="P5310_05325"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32814.1"
                     /translation="MKYTLEEIAKRINGKLSGPPDLVIEGINTPELAGEDEICVVFDE
                     NLISSAENGSAGAVVIKDELSVAKPYITVEDPRSVLPTLLKLFLEKRELAGIHSTVIT
                     GANCNISNNCYIGPYVVIGDNVKIGEKVNIMAGARIGSNVVVGEETFIGYNVVIE"
BASE COUNT          962 a          411 c          686 g          786 t
ORIGIN      
        1 atatatgtag ccttgttgat tgtaatgttt gttactatgt ctggtacatt tgctatttca
       61 agtgataaaa tcaagatatc cggcattgaa gtacagggaa acagcaggat ttctaaagat
      121 gaaattataa agttactgga actcccggat gaagttgatg aagaaattct taaggctggc
      181 ctgcaaaaac ttatagatac tggatatttt tataatgttg ataccagcat aaaagtggtg
      241 gataataaat atatactggt attgaatgtg gtggagcttc ctgcatttaa aggggtcgag
      301 gtaacaggaa acagggagat aagcacagat gagataaaaa atcttatctc cttaaagcct
      361 ggagatgcta taaatcttac cattttaaga tctgatttgg aaaagattgc aaatctttac
      421 aaagaaagag gatatgtggg ggctaatttc cagttcagta tgacagcgga tggattagta
      481 accgtaaata tcaatgaggg tcctgtggta tctgactttg aatttaaagg aaacgaagtc
      541 cttaaatctg aggagcttgg aaaggcatta gataaatata aaggcagaac actttctata
      601 aatcttctta aggaaatggt aacctgcata caggagttat acaataagaa tgggtatcct
      661 gcctgtgtaa ttttaaacgc atacagtaat gattcgggag ttgtgacctt cgagatagga
      721 gagggaaaga tcagtaagat agaaattaaa ggtaacaata agactaaaga ttacgtaatc
      781 ttaagagaaa tggagaccaa agttgggagt gtcttaaatg ctgataagtt acagaaagac
      841 cttcagaaga tatttaacct tgggtatttc tcagatatta aagccgatat tcatgcaagc
      901 gaaacccccg gagagattat cctgactatt gaggtagtgg aacagcttac cggacaggct
      961 aatggtggac ttgcctatag tacgagagat gggctttctc tgctgcttgg aataagtgat
     1021 tccaatctac ttggaacagg tagaaagttg gggctgtcac tcaatatagg gcttaccgct
     1081 cgtgacatct ctcttaacta tacagaacca tacatattcg gagacctttc aaccctggac
     1141 tgtcttctta tctcgagaca ggcagaaaat tcggatataa tcggtacctc tacggttaaa
     1201 tataatgagg atagaaagac cttggatttg acattgagca gaccgattga tagtaatctc
     1261 cgaggtaccg ttggtgtatc ctgtaatgat atcatatatt ctccagatga agatggaact
     1321 actctcccag atatactcca atctggttta tcaaatgcta ttcttcttgg agttgccaga
     1381 gacaccaggg atgtagtctt caatccgact aaaggtgatt attacggcac aagtataaaa
     1441 tttggaggag gaattttagg tggagatttt agttttacca aacttaccct ggacatgcga
     1501 tggtataagc cagtaacgga taaagaaaca gtagcgataa attatacctg gaattttgga
     1561 tctggcaatc ttccatatat agaaaagtca agcgtcggtg gagtaaactc agtcagggga
     1621 cttccggaga attggaagaa atctgacaat acagagatat taagtttcga atataggatt
     1681 aagatacagg agaatgttag tggggtagta ttcctggatt ttggaaatgg atgggatagt
     1741 gatgaaacta taaatcccac tgacttgtat acagggatag gactgggatt gaggattcca
     1801 ttacccccta tgggaatctt gagactggac tacggatggg gcagttatga ctggcaggga
     1861 agattctatt tcagtatagg acataaattt taactatagg aggaagaaag atgaagaaca
     1921 agatacttat catctcagtg ttagttgttg gaatagcagg aatcgtgtta tggggattag
     1981 gtatccctag gtatggattt gccctggata cgtttaaggt cgggtatata gattacaata
     2041 aggtgataga aagtcatccc aggatggaaa aggcaaagaa agacctgagt gattttgcac
     2101 agaaaactca gaccgattat cagaagcagc tggatgaggc agtaaaaaat aagactgcta
     2161 acgaggctca acagattaag gctgagtatg agtctagatt aaatgaaacc gtaaataaga
     2221 gatggcagga gttattaaat ccaataatca acgatgttaa aaaggcagta agtgatgtgg
     2281 caaagaagaa tgggatagat gtcgtcttaa ggggagacat agttataaca ggtgggattg
     2341 atattaccga tgatgtaata aaagtaataa agaaatgaaa tatactctgg aagagatagc
     2401 taaaaggata aatggtaagt tgtctggtcc accagatctg gttatagagg gtataaatac
     2461 ccctgagcta gctggtgaag atgagatatg tgtagtcttt gacgaaaatc tcatcagttc
     2521 ggcagagaat ggttcagctg gagctgttgt aatcaaagat gaactatctg tggctaaacc
     2581 ttatataact gtagaggacc cgagatccgt actaccaact ttacttaagc tatttctgga
     2641 gaaaagagag cttgctggta tacacagtac tgtaataaca ggtgctaatt gtaatatttc
     2701 taataactgc tatataggac catatgtagt tataggggac aatgtaaaga tcggagaaaa
     2761 ggtcaatatc atggctggtg ccaggattgg aagtaacgtg gttgtaggag aagagacttt
     2821 tattggctat aatgtggtga tagag
//
LOCUS       DAOTKU010000196         2845 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_28737_length_2845_cov_3.046953, whole genome shotgun sequence.
ACCESSION   DAOTKU010000196 DAOTKU010000000
VERSION     DAOTKU010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2845
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28737_length_2845_cov_3.046953"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1402)
                     /locus_tag="P5310_05330"
     CDS             complement(<1..>1402)
                     /locus_tag="P5310_05330"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRU32815.1"
                     /translation="PIIEKCKSAKVSQDGYGVIIRDDGLILAYPEESEILKTNLLQSD
                     SESLKKMIEKALNGEMGVSRYDYEGIRKIGAYTPLGINKWVFWVQEPETSIFAQASAM
                     LNFILIVFIVTTLAVLIVSYLVGVNIADPIVKLAGIADSIAQGNLATKIEGNFRSETA
                     RLAESLAKMVDNFSAIIITAEETSNDISSTGNQINDFTNQTVQAIQQISATIQGIANG
                     AQETARNIQEVSQAVESMSERVVGLAKDAETVSNSNNETVRLVQEGQLVVDDLNKGFN
                     DISSATGSVVDATSELERLANEIGRIVEAITAISSQTNLLALNAAIEAARAGEAGRGF
                     AVVADEVRKLAEESSRSAQQIAAFIEQVRSQIKRTAESINNTLAIMDRQVEIGSRVTD
                     TFVNIREGAESALRAVQGITLTISSLTEESRRISESVQSIAAIAEENAASTEEVSAAV
                     EEVNASVEEINKSISNL"
     assembly_gap    1403..1412
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1413..1896)
                     /locus_tag="P5310_05335"
     CDS             complement(<1413..1896)
                     /locus_tag="P5310_05335"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32816.1"
                     /translation="MFELGRKGSLKTNLIVWFLILSLVPLSLLGYLGYSRFKSSLEDV
                     NVKALSNLSYTQAEAIRNLLEENLSKLEMISGRTDLIMSRLEEYAKRNPNWESIFMTD
                     NRGMGLTTNGTSIDVSDRDYFKTVMSTAKSMISSDISPDTKTPVIVLAAPIVDPSGNP
                     Y"
     gene            complement(2060..2578)
                     /gene="pyrR"
                     /locus_tag="P5310_05340"
     CDS             complement(2060..2578)
                     /gene="pyrR"
                     /locus_tag="P5310_05340"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HRU32817.1"
                     /translation="MIKAYIMDEESIRRALLRITHEILEHNEDIKNIALVGIRTRGVY
                     LAQRIAENIKAIEEVDIPVQFLDVTPYRDDVTRELPKQNLYLDVVNKNVIIVDDVLYT
                     GRTVRAAMDALIDYGRPATIQLAVLIDRGHRELPIRPDYVGKNVPTSRKERVTVHLEE
                     VDGKDEVLIEEE"
     gene            complement(2575..>2845)
                     /locus_tag="P5310_05345"
     CDS             complement(2575..>2845)
                     /locus_tag="P5310_05345"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015591536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HRU32818.1"
                     /translation="GIDISKQRSKTINEFKGITFDYVVTVCDNAKERCPFFPGKKILH
                     KSFIDPTEFKGEGEEKLKIFREVRDQIREWIIETFGKEIKGGDIL"
BASE COUNT          793 a          679 c          433 g          930 t
ORIGIN      
        1 caaattactt atactcttat ttatctcttc tacagaggca tttacctctt ctactgcagc
       61 ggaaacctct tcagtggagg cagcattctc ctctgctata gctgcgatac tctgcacaga
      121 ttctgatatt cttctacttt cttcagtaag agaggagatc gtcagagtta taccctgaac
      181 agctctaagg gctgattcag ccccttctct tatgttaaca aacgtgtcag taactctgga
      241 acctatttct acctgtctat ccattatagc caatgtatta tttatactct cagctgttct
      301 ctttatctga gacctcacct gctcaataaa agctgctatc tgctgagcac tcctactaga
      361 ttcttcggca agttttctta cctcatctgc tactacggca aatccacgtc cagcctcccc
      421 tgccctggca gcctctatgg ctgcattgag agctaaaaga ttagtctgag aacttatggc
      481 agttatagcc tcaacaatac gacctatctc attggcaagt ctctcaagtt cagatgtggc
      541 atcaaccaca gaccctgtgg cagaggagat atcattgaac cctttattta aatcgtccac
      601 tacaagctga ccttcctgaa ctaaccttac agtttcattg ttactattcg aaactgtttc
      661 agcgtcttta gccagtccaa ctaccctctc actcatgctc tctacagcct gtgatacctc
      721 ctgtatattt cttgctgtct cctgcgcccc attcgctata ccctgaatgg tagctgaaat
      781 ctgctgaatt gcctgaacag tttgatttgt aaaatcattt atctggttgc ctgtggaact
      841 tatatcattg gatgtctcct ctgctgtgat aattatagcg gaaaaattat cgaccatttt
      901 agccagactt tctgcaagtc tggctgtttc acttctaaaa tttccttcta ttttggtagc
      961 aagattaccc tgggcaatcg aatcagcaat accggcaagc ttaactattg gatcagcaat
     1021 gtttaccccc acaagataag aaactataag aaccgccagg gttgttacaa taaaaacaat
     1081 aagaataaaa ttaagcattg cactggcctg agcaaatata ctcgtctctg gttcctgtac
     1141 ccagaatacc catttattaa tacctaaagg ggtataagca cctatcttgc gtatcccctc
     1201 ataatcatat cttgaaaccc ccatttctcc atttaaagcc ttctcaatca tctttttcag
     1261 actttcagaa tcagactgca acaaatttgt cttcaaaatc tctgactcct caggataggc
     1321 aagaatcaat ccatcatccc tgataataac cccatagcca tcctgagaaa ccttagccga
     1381 tttacacttt tcaattattg gtnnnnnnnn nncatacgga ttaccgctgg gatcaacaat
     1441 aggtgcggca agaacaataa caggggtttt agtatccggt gatatatcac ttgatatcat
     1501 agatttagct gtactcatta cagtcttaaa gtaatctcta tccgatacat ctatactggt
     1561 tccattggtg gttagcccca tccctctgtt gtcagtcata aaaatactct cccaatttgg
     1621 attccttttt gcgtattcct ccaatctgct cattataagg tctgttctcc ccgaaatcat
     1681 ctccagcttt gacagatttt cttcgagaag attccttata gcttcagcct gagtataaga
     1741 caaattagag agagctttta catttacatc ctccagagaa cttttaaatc tggaatatcc
     1801 aagataacct agcagagata gagggacaag agaaaggata aggaaccaca caataagatt
     1861 ggtctttaat gaacccttac gccccagttc gaacattacc agtcacccct ttccacattt
     1921 ttggatatta tatcataaaa atagaacttg gaaaggtaga atggagctta gttttgctgt
     1981 attatcttaa taaagacatc aagaccctgc tagaattttc ctgaaaatat tggagactaa
     2041 gatagagtag gggtatcccc tactcttctt ctattaaaac ttcatcttta ccatctactt
     2101 cttctaaatg tactgttact ctttctttcc tggaagtagg gacattcttt ccgacataat
     2161 caggtcttat gggaagttct ctatgccctc tatcaataag gacagctagt tgaatagttg
     2221 caggtctacc ataatctata agggcatcca tagctgctct taccgtcctt ccagtgtata
     2281 atacatcatc cacaataata acattcttat tgactacatc cagatagagg ttctgtttgg
     2341 gtaattctct cgtcacatcg tctctgtatg gagttacatc taaaaattga accggaatat
     2401 ccacctcctc tatagccttt atattttctg ctatcctttg agctagatat acccctcttg
     2461 ttcttatacc gacaagagca atatttttta tatcttcatt atgttcaagg atttcatgag
     2521 taatcctgag taacgctctc cttatacttt cttcatccat tatatacgcc tttatcataa
     2581 aatatcacca cctttaattt ctttgccaaa tgtctctata atccattctc taatctgatc
     2641 tcttacctct ctaaaaatct taagcttctc ttctccttcc cctttaaatt ctgtcggatc
     2701 tataaaactt ttatgtagaa tcttcttgcc ggggaaaaat ggacacctct ctttagcgtt
     2761 atcgcaaact gtgaccacat aatcaaatgt gattccttta aactcattaa tagtctttga
     2821 tctctgctta gatatatcta tccct
//
LOCUS       DAOTKU010000197         2824 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_29009_length_2824_cov_2.707114, whole genome shotgun sequence.
ACCESSION   DAOTKU010000197 DAOTKU010000000
VERSION     DAOTKU010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29009_length_2824_cov_2.707114"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="P5310_05350"
     CDS             <1..501
                     /locus_tag="P5310_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015740086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRU32819.1"
                     /translation="FAILVSTTFTEPFKEPISYAKYIARLANLLGGGVIVQRLGDLLS
                     GRRSTEDRIKKGIVRPTLSSATPGDLSFVLPYRHLTGILEMLKALDELTPGIYSRYTL
                     LYGVEVKFYSVRPRLNNHLETQIKNLFAIGDGAGVTRGLVQASMAGVISAREIAKRIG
                     VERWEL"
     gene            489..1736
                     /locus_tag="P5310_05355"
     CDS             489..1736
                     /locus_tag="P5310_05355"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013450504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HRU32820.1"
                     /translation="MGAIAVIGLQWGDEGKGRIVDLLVKNCDGVVRYNGGPNAGHTVV
                     VDNEIFKLHLLPSGILHKDKANIVANGVIIDPGVLIDEIENLKQRGFFNGELFISDRA
                     PLLFPYHKILDEIEESLRGSFNLGTTKRGVGPGYTDEVNRSALRITDLLSDSFGEKLR
                     RLWDFKVRLFPVLEGRIEIDRVIDEYHNHLEYLKPYIVDTVDLLHKLLSEGKKLLLEG
                     AQGFLLDIDFGTYPYVSSSSTSIGGVFSGSGISQKQLESVYGVAKAYMTRVGGGPFPT
                     ELKDDTGNYLREKGKEYGTATGRPRRTGWLDCVATRYSCTVNGIDGIYLTKMDILDGL
                     PEIKVCVAYEYEGNRIDYFPASLDILENCTPIYETFPGWRMSEAKLPPDAKSYIKYIE
                     DFLGVNVLGVSIGPEREAFIDLC"
     gene            1760..1835
                     /locus_tag="P5310_05360"
     tRNA            1760..1835
                     /locus_tag="P5310_05360"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1793..1795,aa:Pro,seq:cgg)
     gene            1857..2267
                     /locus_tag="P5310_05365"
     CDS             1857..2267
                     /locus_tag="P5310_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015590386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HRU32821.1"
                     /translation="MKTYTEVSNLRSQKRIEIINVSSQVRQVVERSGVRDGIILIYSL
                     HTTTGLYVNQSERNLETDLETLLGKLVPQQNDYKHNAIDNNADAHLRSILLGNYVALP
                     LTNGDVDLGVWQSILFVEFDGPRRRAFITKIIGE"
     gene            2291..>2824
                     /locus_tag="P5310_05370"
     CDS             2291..>2824
                     /locus_tag="P5310_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HRU32822.1"
                     /translation="MAISEERLKALEGAIAKIEKDHGKGAIMKLGEATSKLQIEVIPT
                     GSLGLDIALGIRGIPRGRITEIFGPEASGKTTLALHIIAEAQKLGGIAAFIDAEHALD
                     PIYARNLGVNIDEMWISQPDSGEQALGIADTLASSGAVDIVVIDSVAALVPKAELEGD
                     IGDQYIGLQARLMSQALR"
BASE COUNT          833 a          461 c          749 g          781 t
ORIGIN      
        1 tttgctatcc ttgtaagtac aacatttact gagccattta aagaaccgat atcctatgca
       61 aagtatatag ccaggctggc aaatctgctg ggtggagggg ttattgttca gagactgggg
      121 gatctcctct caggtagaag gtcaaccgaa gacaggataa aaaaaggtat agtaagacca
      181 acattgtctt ctgcaactcc aggtgacctg agttttgtcc ttccatacag gcatcttaca
      241 gggatactgg agatgctgaa ggctttagat gagcttaccc ctggaatata ctccagatat
      301 acactgctct atggggtaga ggttaaattc tattcagtaa gacccaggct taacaatcat
      361 ttagaaactc agataaagaa tctttttgcc ataggtgatg gggcaggagt gacaagggga
      421 cttgtacagg catctatggc aggagtaatt tctgcaagag aaatcgccaa gagaatagga
      481 gtagagagat gggagctata gcagtaatag gattgcaatg gggagatgag ggaaagggca
      541 ggatagtaga ccttctggtc aagaattgtg atggggtggt aaggtataat ggtggtccga
      601 atgctggaca cacagtggtg gttgataatg agatctttaa gcttcatctt ctcccctcag
      661 gaattcttca taaagataag gctaatatag tggctaatgg ggtgataata gacccagggg
      721 tattgataga tgagattgaa aatcttaaac agcgaggatt ctttaatgga gaacttttta
      781 tctctgatag agcccctttg cttttccctt accataagat tcttgacgag attgaagagt
      841 ctctgagagg ttcttttaac cttggaacga caaaaagggg ggttggacca ggttatactg
      901 atgaggtgaa tcgttctgca ttaagaatta cagaccttct ttctgattcc tttggggaaa
      961 aacttagacg attatgggat tttaaggtca gattatttcc tgttttagaa ggcaggattg
     1021 agatcgatag agttatagat gagtaccata accatctgga gtatctgaag ccatatatag
     1081 tagatacagt ggatctcctc cataagcttc tctctgaagg gaaaaagctc cttttagaag
     1141 gagctcaggg attcctttta gacatagatt ttgggacata tccatatgtg agttcatctt
     1201 caactagtat aggtggtgtt ttttctggtt ctggaataag ccaaaagcaa cttgaaagtg
     1261 tttatggggt agctaaagct tatatgacca gggttggagg aggacctttc cccactgaat
     1321 taaaagatga taccggtaat tatctgagag aaaagggtaa ggagtatggt actgctaccg
     1381 gtaggcccag gaggactgga tggttggatt gtgtggctac aagatactcc tgcacagtaa
     1441 acgggataga tggtatatat cttacaaaga tggatatcct ggatggtcta cctgagatta
     1501 aggtatgtgt ggcttatgag tatgaaggta ataggattga ttatttccct gcttctcttg
     1561 atattttaga aaactgtacc cccatatatg agacattccc gggatggagg atgagcgaag
     1621 caaaacttcc tcctgatgca aagagctata taaaatatat agaagatttt cttggggtta
     1681 atgtccttgg ggtaagcata ggtccagaga gagaagcttt tatagattta tgttaaattt
     1741 tctgatataa ttatcttggc ggggcgtagc gcagttggga gcgcgcctgt ttcgggtaca
     1801 ggaggtcgaa ggttcgagcc ctttcgcccc gaccaattta gattaggagc ttaaggatga
     1861 agacttatac agaggtgtcg aacctcaggt cccaaaaaag gatagagatt ataaatgtaa
     1921 gctctcaggt aagacaggtg gtggagagaa gtggtgtaag ggatgggatt atacttatat
     1981 attccctcca taccactaca ggtttatatg taaatcagag tgaaagaaac ttagaaacgg
     2041 atctggaaac acttttaggg aagctggtgc cacagcagaa tgactataag cataatgcta
     2101 ttgacaataa tgcagatgcg catctcagat ccatactgtt aggtaattat gtggcgctac
     2161 ctctaactaa tggtgatgtt gacctgggtg tctggcaatc tatactcttt gtggagtttg
     2221 atggtcccag gaggagggct tttatcacca agataatagg tgaataatag ggggagagtg
     2281 aagatataag atggctatct cagaggagag attaaaggca ttagagggag ctatagcgaa
     2341 gatagagaag gaccatggga agggcgctat aatgaagctt ggcgaagcta ccagcaagct
     2401 tcagatagag gttataccta ccggttccct gggacttgat attgctcttg gcataagagg
     2461 gattccaaga ggcaggatta ctgagatctt tggtcctgag gcttcaggta aaacgacact
     2521 ggcactccat ataattgctg aagcccagaa gctgggtggt atagctgcat ttatagatgc
     2581 agaacatgct ttggacccta tctatgccag aaatctgggt gttaacatag atgagatgtg
     2641 gatttcacag cctgatagcg gtgaacaggc gttgggtatt gctgatacgc tggcatccag
     2701 tggggcggta gatattgtgg tgatagattc ggtagcagct ttagtcccca aagcagagtt
     2761 agagggagat attggggatc agtatattgg tcttcaggca agacttatgt ctcaagcact
     2821 cagg
//
LOCUS       DAOTKU010000198         2820 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_29058_length_2820_cov_2.968174, whole genome shotgun sequence.
ACCESSION   DAOTKU010000198 DAOTKU010000000
VERSION     DAOTKU010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29058_length_2820_cov_2.968174"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..969
                     /locus_tag="P5310_05375"
     CDS             <1..969
                     /locus_tag="P5310_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH116 family glycosyl hydrolase"
                     /protein_id="HRU32823.1"
                     /translation="GLLYLGALKTCAEIAEKLGEGENAREYLKIFENGRKLLAEELFN
                     GEYLIQKYDRGKDYKYQYGDGCLSDQLLGQWLADTIGLGDLIPGEYIEKTLESIFKYN
                     WRDDLSEHSNCQRTYALNDEKGLLLCTWPRDNREKFPFPYSDEVWTGIEYQVASHLIY
                     RGFLKEGLAMVKGLRGRFDGLRRNPWDEPECGHHYARAMSSWALLLALSGYQYDGRIS
                     FIGFSPKLSTSRFRSFFSTATGWGIFSQDIADNSCKFNISSLYGNIRIRLIKLDIPER
                     FSPEFSVKLITGNGDREIDFKFKQGVIELENTIELKEGEALEVIVF"
     gene            complement(1038..2381)
                     /locus_tag="P5310_05380"
     CDS             complement(1038..2381)
                     /locus_tag="P5310_05380"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HRU32824.1"
                     /translation="MALNSFPDTEDEILKYVKENNIYFIEVWFTDILGYLKSFAITEH
                     ELKKAFEEGLGFDGSAIRGFTRIDESDMMAIPEPETFTVLPWKPEERKVARIFATIVE
                     PNGTPYEGDPRNVLKKALQRAKNMGFNFYVGPELEYFYIKTPFSAPELLDFGGYFDLI
                     PRDEAIDIRHQTVIMLEKLGIDVESSHHEGAPSQHEIDFRYDDAMSMADAVMTARVAV
                     KEAAYMNGVYATFMPKPFSNYNGSGMHVHMSLFGNGHNAFFDQNDKFHLSSMAKYFIA
                     GLMRHAPEITLITNQWINSYKRLVPGYEAPVYISWALRNRSDLIRVPMYKVGKEEATR
                     VEYRAPDPSCNPYLAFAVILSAGLEGIEKEYPLPDPVEENVYFMSEEERKARGIDNLP
                     GSLIEAIEITENSELVKKTLGEHVFKNFIDNKKIEWDNYRKQVTSYELKTYLPLL"
     gene            complement(2469..>2820)
                     /gene="rplM"
                     /locus_tag="P5310_05385"
     CDS             complement(2469..>2820)
                     /gene="rplM"
                     /locus_tag="P5310_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HRU32825.1"
                     /translation="RLASKIASILRGKHKVYFSPSVDTGDFVIVINADKVKVTGKKYT
                     DKLYYRHTLYPGGLKVKSLREFMEEDPSKAIEHAVRGMLPHNSLGEMQFKKLKIYTGG
                     EHPHKAQQPELLEF"
BASE COUNT          831 a          550 c          550 g          889 t
ORIGIN      
        1 ggattgttat acctgggagc tcttaaaacc tgtgcagaaa tagccgaaaa acttggagaa
       61 ggggagaatg cgagagaata tctgaaaatt tttgaaaatg gaagaaaact tctggctgaa
      121 gagttattta acggtgaata ccttattcag aagtatgata gaggaaagga ctataagtat
      181 cagtatggag atggatgtct ttctgatcag cttttaggtc agtggcttgc agatactatt
      241 gggttaggag acctcatacc tggagagtat attgaaaaga ctctggaatc tatttttaaa
      301 tataactgga gagatgacct ttcagaacac tctaactgtc agaggactta tgcactgaat
      361 gatgaaaaag gactcttgct ctgtacctgg ccgagggata acagggagaa atttccattc
      421 ccatattcag atgaggtatg gacggggatt gaatatcagg tagcaagtca tttaatatac
      481 agaggatttt taaaagaagg gcttgcaatg gttaagggac taagaggacg ttttgacgga
      541 ttaaggcgca atccctggga tgaaccggaa tgtggacatc actatgccag agccatgtca
      601 agctgggctt tgctattagc tctttctggt tatcaatatg atggaaggat atcgtttata
      661 ggtttttctc cgaagttaag tacgtccagg ttcagaagct tcttctctac ggctaccgga
      721 tggggaatat ttagtcagga tattgcagat aactcctgca agtttaatat ctcaagttta
      781 tatggcaata taagaattag gcttattaag ctagatatac cggaaagatt ctcccctgaa
      841 ttctcagtaa agcttattac aggtaatggg gatagagaaa ttgattttaa gtttaaacag
      901 ggagtaatag aacttgaaaa tacgatagag ttaaaagaag gggaagcgct ggaggtgatc
      961 gtcttttaag tgttccacag tggaaatatc gctgaaatga aagagggggg agataacagt
     1021 ctccccacct cttaattcta taataaaggt agatatgtct tcagttcata acttgttacc
     1081 tgcttacgat aattatccca ctctatcttt ttattatcta taaagttttt aaagacatgt
     1141 tctcccagtg tcttctttac cagttcacta ttttcagtaa tctctatagc ctctataaga
     1201 ctaccaggaa gattatcaat tcctcttgct tttctttctt cttcagacat aaaatataca
     1261 ttttcttcta cagggtctgg caatggatac tctttttcta tcccttcaag accagcagat
     1321 agtattactg caaatgccag gtatggatta catgatggat caggagctct atattctact
     1381 cttgtggctt cttcttttcc tactttatac atgggaaccc ttatgaggtc tgatctgttt
     1441 ctgagagccc aggatatata tacaggtgcc tcatatccag ggaccagtct cttataagaa
     1501 tttatccact gatttgttat aagagttatc tcaggagcat gtctcattag tcctgctatg
     1561 aaatatttag ccatgctgga tagatgaaat ttatcatttt gatcgaagaa tgcattatgc
     1621 ccattcccga acaggctcat atgaacatgc atacctgaac cattgtagtt tgaaaaaggc
     1681 tttggcatga atgtggcata aactccattc atataagcag cttcttttac agcaaccctg
     1741 gcagtcatta cagcatcagc catactcata gcatcatcgt atctgaagtc tatctcatgc
     1801 tgagatggag ctccttcgtg atggcttgat tctacatcta taccgagttt ctccagcatt
     1861 ataactgttt gatgccttat atctatcgcc tcatccctgg gaattaaatc aaaatatcct
     1921 ccaaagtcaa gaagttcagg cgcagagaat ggggttttta tataaaaata ttccagttca
     1981 ggtccaacat agaagttaaa ccccatattt ttagccctct gtaaagcctt tttgaggaca
     2041 tttcttgggt caccctcgta cggagttcca ttaggctcca ctatagtggc gaatatcctt
     2101 gccacctttc tctcttcagg tttccatggc aggactgtaa aggtctctgg ctcaggtata
     2161 gccatcatat cgctctcatc tattctggta aagcctctta tggctgagcc atcaaaccca
     2221 agtccctctt caaatgcttt tttcagttca tgctcagtaa tggcaaaact cttcagatag
     2281 ccaagaatat cggtaaacca gacttctata aagtatatat tattctcttt tacatacttg
     2341 aggatttcat cctcagtatc tggaaaacta tttaaagcca taccatcctc ctataataaa
     2401 taattacggg cttactagcc cgtaattata ccacgaggaa tatggacaga caagttagaa
     2461 tctaaaaatt aaaactcaag aagctcaggt tgttgagcct tgtgaggatg ttcaccacca
     2521 gtgtatattt ttagcttttt aaattgcatc tctcccaggg aattatgagg aagcataccc
     2581 ctgaccgcat gttctatagc cttactcggg tcctcttcca taaattctct gagagacttt
     2641 acctttaatc cacctggata tagggtatga cgatagtaca gcttatctgt atatttctta
     2701 cctgtaactt tcaccttgtc tgcgttaata actataacaa aatctccagt atctacgctt
     2761 ggagagaaat ataccttatg tttacctctc agtatactgg cgatcttact tgccaatcta
//
LOCUS       DAOTKU010000199         2812 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_29167_length_2812_cov_3.338411, whole genome shotgun sequence.
ACCESSION   DAOTKU010000199 DAOTKU010000000
VERSION     DAOTKU010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29167_length_2812_cov_3.338411"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            151..621
                     /locus_tag="P5310_05390"
     CDS             151..621
                     /locus_tag="P5310_05390"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HRU32826.1"
                     /translation="MSVNGSSIEIEKISEVLKPIFMEYPYIAAAYLFGSQVKGKTTPM
                     SDIDIAILIKDNAPKGRELIHEEDYLSYRIEKVLKVGEIDLVELNSQGLIFQHNVLKT
                     GRLIYDGDPEFRIRFESRVISNFCDFEVTLRFIEKHHTEGRMARCKRLFKIVQG"
     gene            660..983
                     /locus_tag="P5310_05395"
     CDS             660..983
                     /locus_tag="P5310_05395"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HRU32827.1"
                     /translation="MNIKEIPVLTELKVKLKELLGSRPFSIILYGSKARGDYDSDSDT
                     DVAIIVEGLTRELKDQILDVVTALELEYLTPLSTFIISKEEFEHLKNRERRIALDIER
                     EGIEI"
     gene            1013..>2812
                     /locus_tag="P5310_05400"
     CDS             1013..>2812
                     /locus_tag="P5310_05400"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HRU32828.1"
                     /translation="MDFPIIKTDIANSKRIAIILAAGKGKRMGDPGRNKVCYPIAGIP
                     VINRIIETYKHCGFQDIIVVVGYRYEDVISVVNERFSDINFIYQEQPLGTGNAVKCAS
                     RYLESVGFNGDIFISAGDKLVDGNLLVEMRETFESGDYDLLFATGTSSGGEFGRVIRD
                     DTGRIVSIVEAKDIELLKKQGREDILDLVEKSDETNQSLYFIKAPLLYSALKEIKSNN
                     AQQEEYITDIVNILSKAGAKIDTFLVTDKDKILSFNTPQELLEIEMYFQRIKLKELNG
                     NLRNPANYKMVSEWIEIFTRKDRNLLKLLADIYRDDTSIIDKKINMYLKVLDEFRKRF
                     GPDRYVSIIRSPGRLNIMGRHIDHRGGAVNIIAIDKEIIMVAEPRKDDIINLYNIQED
                     KFPPREFSISKEMSKLDWSSWLNAINSDGLKEELNKNSGDWANYVKASALKIQERFRD
                     IKILGMNAVVLGDIPIGSGLSSSSSMVVASAEAITLVNQLNISPEEFVKLCGEGEWYV
                     GTRGGSGDHAAIKFGMKGNISQIKFFEFEFAGSAPLPKGYSIVFCYSQEQAKKAENAK
                     NVFNQKVACYELGTMILRKVFPELSSKMRYLRDI"
BASE COUNT          972 a          408 c          647 g          785 t
ORIGIN      
        1 acttgatttt gataataata tggcaacctg tagagactgt ggaaagaagt ataagttaga
       61 agatgggaat gtaacaaggc ttatgatata attatcaatg aaactgaaag attataaaac
      121 aattgaaggg ggatcagaga aactacaatt atgagtgtga atggttcaag tatagaaatt
      181 gagaagattt ctgaagtcct gaagcctatc tttatggaat atccatatat agcggcagca
      241 tacctttttg gttcacaggt aaaaggcaag acgactccta tgagtgatat agatatagcg
      301 atacttatta aggacaacgc tccaaaggga agagaattga tccatgaaga agattatctt
      361 tcctatagaa ttgaaaaggt gctgaaagta ggagaaattg atctggttga gcttaacagt
      421 caggggctta ttttccagca caatgttctg aaaacaggta gacttatata tgatggtgac
      481 cctgaattta gaataagatt tgagtcccgg gtaatctcta atttctgtga ctttgaggta
      541 actcttagat ttattgaaaa acatcatact gaaggtcgta tggcaaggtg taaaagacta
      601 tttaagatcg ttcagggttg aatagctagt gaataatgaa gaataagacc tttttagata
      661 tgaatattaa ggagatacca gttcttactg agctcaaggt taaactgaaa gaactactgg
      721 gttccagacc tttttctata atcctctatg ggtctaaagc acgaggagat tatgatagtg
      781 attccgatac tgatgtggct atcatagttg aaggtctaac gagagagctt aaggatcaaa
      841 tacttgatgt ggtgactgct ctggaacttg aatatctgac tcctctttct acttttatta
      901 tttcaaaaga agagtttgag catcttaaaa acagggaacg aagaatagct cttgatatag
      961 aaagagaagg aatagagata tgaaagaaga agacatatgg aggtggtgac ttttggattt
     1021 tccaataata aaaactgata tagccaattc taagaggatt gctataattc ttgctgcagg
     1081 aaaagggaag aggatggggg accctggcag aaacaaggta tgctatccta tagcaggtat
     1141 ccctgttata aacagaatca tagaaactta taaacactgt ggttttcagg atataatcgt
     1201 tgtggttggt tatagatacg aagatgtaat ctctgtagta aacgagagat tcagtgatat
     1261 aaactttatc tatcaggaac agcctcttgg cactggaaac gcagttaaat gtgcctccag
     1321 atatctggag tctgtcggat ttaatggaga tatatttatc tccgctggcg ataaactggt
     1381 ggatggcaat ctgcttgtag agatgagaga aacttttgaa tcaggtgatt atgatcttct
     1441 cttcgctaca ggcacatcca gtggtggtga gtttggcaga gtgataagag atgatactgg
     1501 cagaatagta agtatagtgg aagccaaaga tatagaactt ctaaaaaagc agggaagaga
     1561 agatatctta gacctggtag agaaatctga tgagacaaat cagtctcttt attttataaa
     1621 agcaccatta ctctacagcg cactaaagga gataaaaagt aataatgcac agcaggaaga
     1681 gtatattaca gatatagtaa acatattgtc taaagctggt gcaaagatag atacctttct
     1741 ggtaacagat aaggataaga ttctcagttt caatacccct caggagttac tggagataga
     1801 gatgtatttt cagagaataa aattgaaaga attgaatgga aatctcagga atcctgccaa
     1861 ctacaagatg gtatcagagt ggatagaaat ctttaccaga aaagaccgta atctgttaaa
     1921 gctccttgct gatatatata gagatgacac atccataata gataaaaaga taaatatgta
     1981 tcttaaggtt cttgatgagt tcagaaagag atttggaccc gatagatatg tatccataat
     2041 aaggtctcct ggcaggctga atattatggg acgtcatata gaccacaggg gaggagcggt
     2101 aaatatcata gccatagata aagagataat aatggtagct gaacccagaa aagacgatat
     2161 aataaacctc tacaatatac aggaggataa gtttccccca agagaatttt ctatatctaa
     2221 agagatgtca aaacttgact ggtctagctg gcttaatgct ataaatagtg atggtcttaa
     2281 agaagaatta aataagaatt cgggcgactg ggcaaactat gtaaaggcat ccgcattaaa
     2341 gatacaggaa cgatttagag atataaagat tctaggaatg aatgctgtgg tccttggaga
     2401 tatacccata ggttctggtt taagttcttc atccagtatg gttgtggcat ctgcagaagc
     2461 tatcaccctg gtcaaccagc tcaatatatc tccggaagag tttgttaaac tctgtgggga
     2521 aggggaatgg tatgttggca caaggggagg ttctggagat catgcggcga taaagtttgg
     2581 tatgaagggg aatatcagcc agataaagtt ctttgaattt gaatttgctg gaagtgctcc
     2641 attaccaaaa ggttacagca tcgtcttctg ctattctcag gagcaggcaa agaaggctga
     2701 gaatgcaaag aatgtgttta atcagaaggt tgcctgctat gaactgggaa ctatgatttt
     2761 gaggaaggtc tttcccgaac tctcatctaa aatgaggtat ctaagagaca tc
//
LOCUS       DAOTKU010000200         2811 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_29176_length_2811_cov_4.124456, whole genome shotgun sequence.
ACCESSION   DAOTKU010000200 DAOTKU010000000
VERSION     DAOTKU010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29176_length_2811_cov_4.124456"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            121..750
                     /locus_tag="P5310_05405"
     CDS             121..750
                     /locus_tag="P5310_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HRU32829.1"
                     /translation="MEIITLFFTSFLVGFSGASTPGPLMTLVLAQSSIGGWKKSLEII
                     SGHAILEGILLILLLFGLQPILEHPIFLRSFTLLGSVFLIYMGITLFISILNNRIEIK
                     NSDPVKIPLTLAGALTSLGNPYWLLWWITIGVSFITQAKSLLLPGILSFYTGHILADY
                     TWYIFIGILGQGLSLPFWGKVYRYVLYFSSLFLFGFGLYFIYYAIFKIS"
     gene            complement(737..>2811)
                     /locus_tag="P5310_05410"
     CDS             complement(737..>2811)
                     /locus_tag="P5310_05410"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HRU32830.1"
                     /translation="SGTSNVQFKLKIDNPHLSSIDDPYLYRLEIKVYSGSELSDRLVK
                     KVGLREVRYSGSNILINDSPVYIRGILHWGWYPDTVAPIPTEEVIREEISKLKEAGFN
                     LIKHCLYVPIEEYFDIADEMGMLIWEELPLWVPKIDDKIKEKVYKQYNEIVKSIRHHC
                     SIIIWTLGCELDETADYQFLNNLYNMVKGLTDGLIRDNSGSGECYGGLLNENADFYDY
                     HFYTDPPFYKDLLDSFAAQWRKEKPWLFGEFCDSDTIRDIKRLIEVYGELPWWIFPKG
                     FGINWNISYHRQIEKIESNNLSDKLPYLVKSSIDKSLVYRKLVLELVRQYKNISGYVI
                     TGMRDTPISTSGIFNDFGELKYELYLIRTINSDTVVTIQWDNRRRWINGGDRLIRWDR
                     FNYQSGSIIRPHIVISHFGKDHIKNARILWTLEDGKDIIDEGEEYIECNLSPGMVNEI
                     ATLEINTPEVSDITKLTFRVKIYDTDLEEVIAENFWAFWLYPKISINKYKEFSVCLID
                     HCNIFQDVKDSIFSKFNLTSDISNCEVIITTTLNQELIGCIGEGKRVLYIQQGGGYIP
                     VERKPFWRESIQIMEKHPIIRGFDNEKFCSQQWYSLATDTVFTSEGLDRLAGNNGKWY
                     PILRRLDARNFDLDEYLVEVVLGKGLIIASTLRFQGGLGDEANGISLNPAGEYLVFQI
                     LNFLLKIS"
BASE COUNT          775 a          594 c          439 g         1003 t
ORIGIN      
        1 ccctacggga tacagctcat atgttctatt tccttcccct gacattttca cagactattg
       61 acatattttt atcctgtcct ggagctttat gatataattt taatataaaa gagatggatt
      121 atggaaatta taacattatt ttttaccagt tttctggttg gcttttcagg ggcatctact
      181 ccaggtccat tgatgaccct ggtattagct cagagtagta taggtggatg gaagaaatct
      241 cttgagatta tctccgggca tgctatcctg gaggggatct tattaatcct ccttcttttt
      301 ggtcttcaac ctatacttga gcatcctatc tttctcaggt cttttaccct tttaggtagc
      361 gtttttctca tatatatggg tataacactc tttatctcca ttttaaataa caggatagag
      421 ataaaaaact ctgatccggt taaaattcct ttaaccctgg caggagcgtt aacttcactg
      481 gggaatccat actggcttct ctggtggatt accatagggg taagttttat tactcaggca
      541 aagagtctat tacttcctgg aatcctgagt ttttacacag ggcatatact ggctgattat
      601 acctggtata tatttatagg tattttaggt caggggttat cccttccatt ctggggaaag
      661 gtctatagat atgttttata tttctcttct ctgttcctct ttggttttgg tctgtacttt
      721 atatactatg caatatttaa gatatcttaa gcagaaaatt taatatttga aaaactaggt
      781 attcacctgc cgggttaagc gagattccat ttgcctcatc tcctaaccct ccctgaaatc
      841 gaagggtaga tgctattatg agcccttttc ccagcaccac ctctaccaaa tactcgtcca
      901 aatcaaaatt tctggcatct aacctcctta aaataggata ccactttcca ttatttcctg
      961 ccaatctatc taagccttca gatgtaaaga ctgtatcagt agcaagtgaa taccattgtt
     1021 gtgaacagaa cttctcgtta tcaaatcctc taattatagg atgtttttcc ataatttgga
     1081 tcgattctct ccagaacggt tttctttcta ctgggatata tcctcccccc tgctgtatgt
     1141 acaggactct ctttccctct cctatacatc ctattaactc ctggtttaaa gtggtggtaa
     1201 ttataacctc acagttagag atgtcagatg tgagattaaa tttactgaat attgaatcct
     1261 taacatcctg aaatatatta cagtggtcta ttagacatac agaaaactcc ttatacttat
     1321 taattgatat cttaggatat agccagaacg cccagaaatt ttccgctatg acctcttcaa
     1381 gatcagtatc atatatcttg accctgaagg taagtttggt aatatcggat acttccgggg
     1441 tatttatttc taacgttgct atctcgttta ccattccggg agacagattg cattctatat
     1501 attcctcccc ctcatctata atatccttcc catcctccag tgtccataat attctggcat
     1561 tctttatatg gtctttccca aaatgggata tcactatatg tggtcgaata atacttccac
     1621 tctgatagtt aaatctatcc cacctgatga gcctatctcc tccatttatc catcttcttc
     1681 gattatccca ttgtatagta acaactgtat ctgaattaat ggtgcggatt aaatatagtt
     1741 catattttaa ttctccaaaa tcattaaata taccactggt tgaaattgga gtatctctca
     1801 tccctgttat tacatatcct gatatattct tatactgcct gactaattct aataccagtt
     1861 ttctgtatac aagtgattta tctattgaac tttttaccag atatggaagc ttatctgaca
     1921 ggttattgga ctctatcttc tctatttgcc tgtggtagga tatattccaa tttattccaa
     1981 agccttttgg aaatatccac catggcaatt ctccatagac ttctattaac ctctttatat
     2041 ctcttatggt gtcactatca cagaattcac caaagagcca aggtttttct tttctccatt
     2101 gggcagcaaa gctatccaga aggtctttat agaatggcgg gtctgtgtag aagtgataat
     2161 catagaagtc agcgttctcg ttaagcaatc ctccatagca ttcacctgaa ccactgttat
     2221 cccttataag tccatcggtg agacccttta ccatattata tagattattc agaaattgat
     2281 aatcagcagt ttcatctaat tcacaaccca atgtccatat aataatagaa cagtgatgtc
     2341 ttatagattt tacgatttca ttatactgtt tgtagacctt ctcctttatc ttatcatcaa
     2401 tcttaggaac ccataaagga agctcttccc atattaacat acccatttca tcagctatat
     2461 caaaatattc ttctatgggc acatatagac agtgttttat aagattaaag cctgcctctt
     2521 ttaattttga tatctcttct cttataactt cttcagtagg tattggggct acggtatctg
     2581 gataccatcc ccaatggagg attcctcgta tatacactgg agaatcgttt attaagatat
     2641 tacttccact ataccttacc tctcttaacc cgaccttttt aactaaccta tctgatagct
     2701 ccgaaccgct gtataccttt atctccagtc tatagagata tgggtcatct atagacgaga
     2761 gatgaggatt atcaatcttg agtttgaact gtacattact ggtgccagaa t
//
LOCUS       DAOTKU010000201         2805 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_29252_length_2805_cov_3.269091, whole genome shotgun sequence.
ACCESSION   DAOTKU010000201 DAOTKU010000000
VERSION     DAOTKU010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29252_length_2805_cov_3.269091"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..131)
                     /locus_tag="P5310_05415"
     CDS             complement(3..131)
                     /locus_tag="P5310_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32831.1"
                     /translation="MGRLKPVTHKELIRKLRKFGFEGPYSGGKQANIKREEWENSE"
     gene            complement(132..380)
                     /locus_tag="P5310_05420"
     CDS             complement(132..380)
                     /locus_tag="P5310_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004078661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HRU32832.1"
                     /translation="MLSEYIEEALKRAHYEFIEDEESFYGEIKELPGVWATGQTLEEC
                     RENLRGVLEEWIVLSLRRGIPIPKIGDFTICEFQELNI"
     gene            complement(399..1676)
                     /locus_tag="P5310_05425"
     CDS             complement(399..1676)
                     /locus_tag="P5310_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HRU32833.1"
                     /translation="MGDIILVERERGIKEGDLIEALEASLEDYRPLKRVLLLPPDITR
                     KYSFAGRITALYYNLLKSSCHIDIMPALGTHMKMSKEEINEMFGSSIPEDRFIAHNWR
                     NDVVKIGEIPGDFVNGVSEGIIDEPIEVEINRRLLDKDYDLIISIGQVVPHEVSGMAN
                     YTKNIFVGCGGKRTIDKTHLLGAFYGLERILGRIDSPVRKVFDYGEENFLKNIPILYV
                     LTVTTQIDNSSTLWGLFIGRDRKIFEEASELSWEKNITFVERPIKKIVTFLEEREFKS
                     TWLGNKSVYRTRLAIDSGGELIIIAPGVRQFGEDRENDRLIRKYGYAGRDRVIELFRE
                     NRDLRENQSVVAHLIHGSSDGKFTITYAVKHLTQEEVEGVNYRYMPLEEAYNKYKPEG
                     AKEGFNITEEGEEFFFIRNPALGLWISRDRFGN"
     gene            complement(1685..2629)
                     /locus_tag="P5310_05430"
     CDS             complement(1685..2629)
                     /locus_tag="P5310_05430"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HRU32834.1"
                     /translation="MGLRVLVTSRSFGRVSDEPVELLKENGFEVDIISEPTEERILEI
                     IDRYDAIIVGNDKITGDILEKAKNLKIICKHGVGVDNIDLKSAKESGIRVTNVPATNS
                     DAVADLTFGLILDLARRITSTFIRIKSGSWNPLIGVDVHHKTLGIIGLGAIGKRVARR
                     ANGFDMLVLAYDPYIEVLPEEYSFVSLVTIGEVLKNADFLTIHTPLTEKTRGLIGKKE
                     IEMMKNGSFIINAAREGIVDESAVYDFLKSGHLGGAGFDVIDKDSPVKDLLLSLDNVI
                     ILPHMGMYSGEALNSVSMVCARNIIKQFKGEEPENIVV"
BASE COUNT          755 a          715 c          407 g          928 t
ORIGIN      
        1 gtttactcag aattttccca ttcctctctc tttatattcg cctgtttacc accactataa
       61 ggtccttcaa atccaaattt ccttaacttt cttataagtt ctttatgtgt gacaggtttt
      121 aatcttccca tttatatatt taactcttga aattcacata tagtaaaatc tcctatctta
      181 gggataggaa tgccccttct caagctgagc acaatccact cctcgagaac tccccttaaa
      241 ttttctctgc attcttcaag agtttgccct gttgcccaaa ctccaggtaa ttcttttatt
      301 tccccataaa aagattcctc atcctctata aactcataat gtgctctttt caaggcttct
      361 tctatatatt cactaagcat aatcttgcta cacctccttc agtttccaaa cctatctctg
      421 gatatccaga gaccaagggc agggtttctt ataaagaaga actcctcccc ctcctcggtt
      481 atattaaacc cctctttagc cccctcaggt ttatacttat tataagcctc ctccagaggc
      541 atatatctat aatttacccc ctcaacctcc tcctgcgtga gatgttttac tgcgtaggta
      601 atggtaaact ttccatcgga agaaccatgt ataagatggg ctactacaga ctgattttcc
      661 cgcaggtctc tgttctctct aaaaagttca atgaccctat cccttccagc ataaccatac
      721 tttcttatca atctatcgtt ttctctatcc tcaccaaact gcctcacccc gggagctatt
      781 ataataagct ctcctccact atctatagcc agtctggtcc tgtatactga cttattaccc
      841 agccaggtac ttttgaattc tctctcctct aaaaaggtaa ctatcttttt tatcggtctc
      901 tctacaaaag ttatattttt ctcccagctc agctctgacg cttcttcaaa tatctttcta
      961 tccctcccga taaatagacc ccacagggta ctagaattat caatctgtgt tgtaactgta
     1021 agcacataga ggatggggat attttttaaa aaattttcct ctccatagtc aaacaccttc
     1081 cttacaggac tatctatcct gccaagtatc ctctcaagac cgtagaatgc tcccaaaaga
     1141 tgagttttat ctatagtcct tttccctccg cagccaacga agatgttttt ggtgtagttt
     1201 gccattccgg acacctcatg tgggaccacc tgtcctatcg atattataag gtcataatct
     1261 ttatccagaa gccttctgtt tatctctacc tctatcggct catcaattat tccctctgag
     1321 acaccgttca caaaatctcc agggatttca cctatcttta ccacatcatt tcgccagttg
     1381 tgagctataa acctatcctc agggatcgaa ctaccaaaca tctcattaat ctcttcttta
     1441 ctcatcttca tatgtgtccc aagggctggc attatatcta tatgacagct tgatttgaga
     1501 aggttatagt acagtgcagt tatcctccct gcaaaggaat actttctcgt tatatccgga
     1561 gggagtagaa ggactctctt taaaggtcta taatcctcca gagatgcctc cagggcttct
     1621 atcaggtccc cctcttttat ccctctttct ctttctacca gaattatatc tcccatacaa
     1681 tcacctacac cactatattt tcaggttctt cacctttaaa ttgtttgatt atattcctgg
     1741 cacataccat actgacagag tttagcgctt cccctgagta catacccata tgggggagta
     1801 ttattacatt atccagtgac agtaagaggt ccttcacagg agaatcttta tctatcacgt
     1861 caaaacctgc ccctcccaga tgtccacttt tcagaaaatc atatactgca gactcatcaa
     1921 caataccttc tctggcagca tttatgatga aactgccatt tttcatcatc tctatctctt
     1981 tcttacctat caatcctctg gtcttctcag ttagaggtgt atgaattgtc aggaagtctg
     2041 cattcttcag aacctcccct atggttacca ggcttacaaa tgagtactct tcaggaagaa
     2101 cttctatata ggggtcatat gccagaacca gcatatcgaa accattagct cttctggcta
     2161 cccttttacc tatagctcca agacctatta tacccagcgt cttatggtgg acatccacac
     2221 cgatgagggg gttccaggag ccacttttta tccggataaa agtgctcgtt attcttcttg
     2281 ccaggtccag tataagtcca aacgttaaat cggctaccgc atcagaattt gtagcgggaa
     2341 catttgtaac tcttatacca gattctttgg cacttttcag gtctatattg tccaccccaa
     2401 caccatgttt acatatgatt ttaagatttt ttgccttttc aaggatatct ccggttatct
     2461 tatcattccc cacaataatg gcatcatacc tatcgattat ttcaagtatc ctctcttctg
     2521 ttggttcact gattatatcc acctcaaatc cattttcttt tagaagctct actggctcgt
     2581 cacttaccct tccaaatgac ctcgatgtta caagaaccct taaacccata gataagaccc
     2641 tccaaaagta tgctattgat attaattata gcaagatttg agctataatt aaactgattt
     2701 agataactca ggaggatgat atctatgatt gagtatacga atattaaaag cgcatccaga
     2761 gaggaattca ttgatataac cggaaggatt cagaaaatta tagac
//
LOCUS       DAOTKU010000202         2801 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_29301_length_2801_cov_3.248725, whole genome shotgun sequence.
ACCESSION   DAOTKU010000202 DAOTKU010000000
VERSION     DAOTKU010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29301_length_2801_cov_3.248725"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /locus_tag="P5310_05435"
     CDS             complement(<1..701)
                     /locus_tag="P5310_05435"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015907831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HRU32835.1"
                     /translation="MELPQVDIGIFGGSGFYSFLDDLKPYKIETPYGAPSSQIMIGEV
                     SGKRVAFIPRHGEQHQYPPHMINYRANLYAFKLLGVKRIIGPCASGSLKPEIKPGDIV
                     ICDQFVNLTSGRKDTFYDGPITTHISCADPYCPQLRNIIIDCCKRLGFSFHPTGTVVV
                     IQGPRFSTRAESKWFRSIGWETINMTQYPEAVLARELEMCYANISIITDYDTGLEDDP
                     DIKPVTHDEVIKIFQG"
     gene            complement(726..1190)
                     /locus_tag="P5310_05440"
     CDS             complement(726..1190)
                     /locus_tag="P5310_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012963025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HRU32836.1"
                     /translation="MDYKFRKAKMPDVPDIQQMINIYAKEGKMLPRSLNELYENIRDY
                     WVVTIDEAEVVGCCALHPVWEDIGEVRSLAIKEEHTGKGLAKKLVEICLKEASDLSLK
                     RVFALTYIPEFFAKIGFIQVSKEELPHKVWNDCIRCIYFPNCNEVAVVHEVK"
     gene            complement(1193..1537)
                     /locus_tag="P5310_05445"
     CDS             complement(1193..1537)
                     /locus_tag="P5310_05445"
                     /inference="COORDINATES: protein motif:HMM:NF015721.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HRU32837.1"
                     /translation="MKGEVNVDISPNVIVSIASKAAMEVKGVYGLETPSNVSFLRRPQ
                     PHGVKVGISDNKIRLEIYIIAEEGFYLPEVGLNLQEHIKEEVERTTGMNVERVDVYIQ
                     GIHFSKSEEVEE"
     gene            complement(1503..>2801)
                     /locus_tag="P5310_05450"
     CDS             complement(1503..>2801)
                     /locus_tag="P5310_05450"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF006367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HRU32838.1"
                     /translation="KTVLVFSEADRYSLGVKHADEAFCIGPPQTSKSYLNIPNIIAAA
                     EISGADAIHPGYGFLAENADFAEVCESCGIKFIGPSSRVISLMGDKIAARKVMYEAGV
                     PVIPGTFEPVEAEKSALSVAKEFGFPLIIKAAGGGGGRGMRLVRDENELKGALMVAKS
                     EAEAAFNNGSVYMEKYIEGARHIEVQVLGDGRGNVVHFFERDCSIQRRHQKLIEESPA
                     TGIPQRVKEKILESAVIGAKAINYESAGTLEFLVDENDNFYFMEMNTRIQVEHPVTEM
                     VTGHDLVKYQILVAGEESLPIKQEDIVQRGHAIECRINAEDPYNSFLPSTGTLKLFSL
                     PGGPGIRIDTAIYNEIEITSYYDSLIAKLISWGLNRDDAIARMRNALGEFLIEGLKTT
                     IPLYLQIMDDPEYISGNISINFLEERGFLNEGRSERRYLS"
BASE COUNT          796 a          682 c          425 g          898 t
ORIGIN      
        1 ccctggaaga tcttaattac ctcatcatgg gtaactggtt ttatatccgg atcatcctca
       61 agtccagtat cataatcagt aatgatggat atattggcat agcacatctc taactctctt
      121 gccaagacag cctcaggata ttgtgtcata ttaatagttt cccagccaat acttctaaac
      181 cacttactct ctgcccttgt actgaatctg ggaccctgaa ttaccaccac cgttccggtt
      241 ggatgaaagg aaaatcctaa tctcttacaa caatcaataa ttatattcct tagctgcgga
      301 cagtatgggt ctgcacagct gatatgagta gttatcggcc catcgtagaa tgtatccttt
      361 cttccactgg taagatttac gaactgatca catataacaa tatcccccgg ttttatctct
      421 ggtttcagac taccagatgc acagggtcca atgatacgtt ttacccctaa aagcttaaaa
      481 gcataaaggt ttgcccgata gtttatcata tgtggaggat actgatgctg ctctccatgc
      541 cgtggtataa aagccaccct tttcccggat acttctccaa tcattatctg ggaacttggc
      601 gcaccgtatg gtgtttctat tttgtaaggc ttaagatcat ccagaaatga atagaatcca
      661 gaaccaccaa atatacctat atctacctga ggaagctcca ttaaatatcc ctcctttaat
      721 cgactctact ttacttcatg cactaccgct acttcattac agtttggaaa atatatacat
      781 cttatgcagt cattccatac cttatgagga agttcttcct ttgaaacctg aataaagcct
      841 attttggcga agaattcggg tatataagta agtgcaaata cacgttttaa agaaagatca
      901 ctggcttctt taaggcagat ctctactaac ttctttgcca gaccttttcc ggtatgctct
      961 tcttttatag ctaaagacct cacttctcct atatcttccc atactggatg taacgcacaa
     1021 caacctacta cttctgcttc atctattgta acaacccagt agtctctgat gttttcataa
     1081 agctcattaa gagatctggg caacatcttc ccttctttag catatatatt tatcatctgc
     1141 tggatgtctg gaacatctgg cattttagcc tttctgaatt tatagtccat actcactcct
     1201 ctacctcttc tgatttagaa aaatgaatcc cctggatata gacatctaca cgttctacat
     1261 tcataccagt agtcctttca acctcctctt ttatatgttc ctgaaggttg agccccacct
     1321 ctggtagata aaatccctcc tccgctataa tgtagatttc aagtctaatc ttgttatctg
     1381 aaatacctac ctttactcca tgtggttgag gtcttctcaa aaaagataca ttggatggag
     1441 tttctagacc atatacccct ttaacttcca tagcagcttt gctggctata gaaacaataa
     1501 cattaggaga gatatcgacg ttcacttctc ccttcattta aaaatcccct ttcttcaagg
     1561 aaattaatcg atatattacc tgagatatac tcaggatcat ccattatctg gagatataaa
     1621 ggaatagtag tttttagacc ttcaatgaga aactctccga gtgcattcct catacgagct
     1681 atagcatcat ctctatttaa tccccacgag ataagtttag ctatgagaga gtcataataa
     1741 gacgtaattt ctatctcatt gtagatagcg gtatctattc ttatacctgg acctcctgga
     1801 agagagaata actttaaagt acccgtactt ggtaagaaac tgttataagg atcttctgca
     1861 tttatccgac actcaattgc atgtcctctt tgaacaatat cttcctgttt tatcgggagt
     1921 gattcttcac cggctaccaa tatctgatac tttaccagat catgccctgt aaccatctcg
     1981 gttacaggat gttcaacttg aatcctggta ttcatttcca taaaatagaa attatcattc
     2041 tcatctacca gaaactctaa agtacctgca ctctcataat ttatagcctt agctccaatc
     2101 actgcagact ccagtatctt ctctttaact ctctgaggaa tacctgtagc tggactctcc
     2161 tcaataagct tctggtgtct tctctggata gaacaatctc tctcaaagaa atggacaaca
     2221 tttccccttc catccccaag aacctgaact tctatgtgcc ttgctccttc tatatacttt
     2281 tccatataaa cagaaccatt attaaaagca gcctctgctt cgctttttgc gaccatcaat
     2341 gcccctttta attcattctc atctctaaca agtctcatcc cccttccccc ccctcccccg
     2401 gcagctttta ttataagagg aaatccaaat tctttagcaa cagacagagc tgatttttca
     2461 gcttctacag gctcgaatgt tccgggtatt acagggacac cagcttcata catcactttt
     2521 cttgcggcaa ttttatcacc catcagtgag ataacccttg aggatggtcc gataaatttt
     2581 ataccacagc tctcacacac ctcagcaaaa tcagcatttt cagctaaaaa tccataccct
     2641 ggatgtatag catccgctcc agatatctct gcagcagcta taatatttgg tatatttaaa
     2701 taactcttac tggtctgagg aggtcctata cagaaagctt catcggcatg ttttacccct
     2761 aatgaatatc tgtctgcctc agaaaacact aaaactgttt t
//
LOCUS       DAOTKU010000203         2800 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_29318_length_2800_cov_3.198179, whole genome shotgun sequence.
ACCESSION   DAOTKU010000203 DAOTKU010000000
VERSION     DAOTKU010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29318_length_2800_cov_3.198179"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1248
                     /locus_tag="P5310_05455"
     CDS             <1..1248
                     /locus_tag="P5310_05455"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HRU32839.1"
                     /translation="ETSNIDSIAEESLLFRNYFATAPLCSPSRGSIMTGRYPGTTGFM
                     GLVNRGWELSLNEKTLPQYLKEAGYSTYLFGFQHERRDPVTLGYDYVAGWDEPPHLAR
                     KITPKVVSFLEGDIKEPFFACVGFFEVHRPWEFYQKIDPKIVNVPDYLPDTPEVREDL
                     AMFYGSILETDYYTGKILETLKRSKIEDNTIFIFTTDHGIAFPRAKSTLYDPGIKTTL
                     ILRWVGGDLTGGKVYDQLLSNVDLLPTILDILEIDIPESIQGISFKPLLYGKDYKPRS
                     EIFAQKDWHDIYDPIRCIRTDRFKYIRNYVEGVWPYLPLPLDIEESLSRKSLGNSHIM
                     RTREKEELYDLSVDPLEINNLSSKPEYRDILDELRSRLYRWQREINDPILQGPIPVPG
                     RGFVQDPVPMFEKKDDGVTGKMI"
     gene            1295..2671
                     /locus_tag="P5310_05460"
     CDS             1295..2671
                     /locus_tag="P5310_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HRU32840.1"
                     /translation="MARSPLDHPNIILINCDDLGYGDLGCYGSKYNRTPFLDKMAKNG
                     VRFTDFYMASPVCSPSRGAMLTGCYPRRVGFGSFEGLLVLFPGQPVGLNTEEITIARV
                     LKQADYATKIVGKWHCGDQPEFLPTRHGFDSYYGLPYSNDMGCQVGAFQYPPLPLLRD
                     EEVIQEQPDQASLTERYVEESVRFIREHKDEPFFLYLAHMYVHLPIYVPWRFLKSSIN
                     GPYGAAVECIDWSVGVLLYELRRLGLEENTLVIFTSDNGSRDRSEGGSNAPLRGTKST
                     TWEGGQRVPCIMYCPGVIPAGITCPELATAMDFLPTLARLAGVKIPEDRIIDGKDILD
                     LMRGIPGAHSPYDAFYYYYMDNLEAVKSGPWKLHISRNRQPVCELYNLEKDIGETADV
                     AGSYTDIVKMLMEKADLIREDIGDAITGVTGNNCRPIGRVDKSRPLTQYDEENPYIMA
                     LYDLPYVG"
BASE COUNT          861 a          485 c          646 g          808 t
ORIGIN      
        1 gaaacttcaa atatagactc tatagctgag gaaagtcttt tattcagaaa ctattttgct
       61 accgctcccc tgtgcagtcc aagcaggggg agtataatga cggggagata tcctggaact
      121 accgggttta tgggacttgt aaatagagga tgggagttat ctctcaatga aaagacatta
      181 cctcagtatc tgaaagaggc aggctactca acatatcttt ttggcttcca gcatgagagg
      241 agagaccctg tgacattggg ttatgactat gtcgcaggat gggacgaacc tccgcatctg
      301 gcaaggaaga ttacccctaa ggtggtatcc tttttagaag gagatattaa agaaccgttt
      361 tttgcctgtg tgggtttttt tgaggttcat agaccatggg agttctatca aaaaatagac
      421 cccaaaattg taaatgttcc ggattatctt cccgatacac ctgaggtaag agaagacctg
      481 gctatgttct atggttccat cttagaaaca gactattata caggtaagat attggagacg
      541 cttaagagga gtaaaataga agataacaca atttttatat ttactacaga tcatggtata
      601 gcttttccaa gagccaagtc gacactatac gatcctggaa taaagacaac cctgatactg
      661 agatgggttg gaggggacct aactggtgga aaagtttatg accaacttct gagtaatgtt
      721 gacctcttac ccacaatcct ggatattctg gagatagata tccccgaatc aatacagggt
      781 ataagtttta agccccttct ttatggaaag gattataaac ctcgtagcga aatctttgct
      841 caaaaagact ggcacgacat atatgaccct attagatgta taagaacaga cagatttaaa
      901 tatataagaa actatgtaga aggcgtatgg ccatatctcc cccttcccct ggatatagag
      961 gaaagccttt ccagaaagtc tctgggcaat tctcatataa tgagaaccag agaaaaggaa
     1021 gagttatacg atttatccgt tgatccgctg gaaataaata atctatcctc aaaaccagaa
     1081 tatagagata tacttgatga attaagatcc agactttaca gatggcagag agagattaat
     1141 gaccctatac ttcagggacc tattcctgtc ccgggtagag gctttgtcca agaccctgta
     1201 ccgatgtttg aaaagaagga tgatggggtt acagggaaga tgatatagga ataaattata
     1261 attcaacttt ttgagacttg aaggaggaga ttttatggct cgttcaccac tagaccatcc
     1321 caacattatt ttgattaact gtgatgattt gggttatgga gatctgggtt gttatggttc
     1381 caaatataac aggactccat tcctggataa aatggcaaag aatggcgtca gatttaccga
     1441 tttttatatg gcatctccgg tatgttcccc atctcgaggt gcaatgttaa caggatgcta
     1501 tccaaggaga gttggttttg gaagtttcga gggattgctg gtccttttcc caggacagcc
     1561 agtaggatta aacactgaag agataactat tgctagagta ctcaaacagg cagattatgc
     1621 tactaaaatt gtaggaaaat ggcattgcgg agatcaacca gagtttctac ctaccagaca
     1681 tggtttcgat agttattatg gcttacctta cagcaatgat atgggatgcc aggttggagc
     1741 atttcagtat cctccactac cacttttacg ggatgaagag gttatacagg aacagcctga
     1801 ccaggcttcg ttaactgaga ggtatgttga ggagagtgtt cgttttatcc gtgaacataa
     1861 ggatgagcca ttctttttat atctggctca tatgtatgtc catctgccaa tctatgtacc
     1921 ctggcgtttc ttgaaatcat cgataaatgg accttatgga gcagctgtgg agtgtattga
     1981 ttggagtgtt ggagttttac tctatgaact cagaaggttg ggtttggaag agaatacgct
     2041 tgttatcttc acttctgaca atgggtcaag agatagatca gaaggtggaa gtaacgcacc
     2101 attacgaggt accaagagta caacgtggga aggtggtcaa agagttccct gtataatgta
     2161 ttgtcctggt gttataccag ccgggataac ctgtcctgaa ttagcgactg ctatggattt
     2221 tttgcctacc cttgcaaggt tagctggggt gaaaattcca gaagatagaa ttattgatgg
     2281 taaggatatc cttgacctga tgagaggtat acctggcgca catagccctt atgatgcatt
     2341 ctattattac tatatggata atttagaggc ggtaaagtca ggaccatgga agcttcatat
     2401 ctcaagaaat agacaacctg tatgtgagtt atacaatcta gagaaggata ttggtgaaac
     2461 tgctgatgtc gctggaagct atactgatat tgttaagatg ttaatggaaa aagcggattt
     2521 aattcgagaa gatataggag atgctattac aggagttaca ggtaataact gtcgtccaat
     2581 tggacgtgta gataaatcca gacctttaac ccagtatgat gaggaaaatc catatattat
     2641 ggctctctat gacctgccat atgttgggta ataggagcca ggatcaaata taaacatggt
     2701 tcttgactgc cagttccttc tgttgtaaaa tatatgagac ttagatatca tatatgatac
     2761 ttaagagagg ttaaatggaa aaaagtttag ttcctggctt
//
LOCUS       DAOTKU010000204         2789 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_29466_length_2789_cov_2.716898, whole genome shotgun sequence.
ACCESSION   DAOTKU010000204 DAOTKU010000000
VERSION     DAOTKU010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29466_length_2789_cov_2.716898"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="P5310_05465"
     CDS             <1..289
                     /locus_tag="P5310_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32841.1"
                     /translation="KASELAKRIHNIIYTRSTPEARKILSLVQQAQRTTDPKKRLELY
                     RQANKIRDINMYSSFPLVQPMSARLVKSNFEVPFNKYLVGFPVGFAYIRTK"
     gene            356..1330
                     /locus_tag="P5310_05470"
     CDS             356..1330
                     /locus_tag="P5310_05470"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRU32842.1"
                     /translation="MTQYIIKRIGWIIFNLLVLSLIAFILIKNVPGSFLELQILIGQA
                     GTMGTGLTISEVEKVRGVETEMMKRWGEDVPLWQQYLTFIKGLVTLDMGPSFRWPTQT
                     IEEIIANTFPVSFTIAILAVTIAIIIGIPLGVLAALKQNSWIDYLVSALVMVVSAIPN
                     YVMAVFVIFLFSFWIPILPTYGWGQPKNYILPVLAMSFGPIGSIAGYMRTSLLDALKQ
                     DYIRTAWAKGGTERHVIFGHAMKNSLIPLVTILGPMISHLILGTVFVEGMFGVPGMGQ
                     YFSQAAGSRDYPLMMAQTVIFGLILMIMNLIVDIIYFFLDPRIRVSRR"
     gene            1337..2194
                     /locus_tag="P5310_05475"
     CDS             1337..2194
                     /locus_tag="P5310_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRU32843.1"
                     /translation="MGNKKRIGRSYWSNIWEKFKRNKLAVFGLIFVCILILLGISAPW
                     LAPYPYDEVHYEAVWTPPCREFLAGTDQLGRDIFSRLLYSLRNAIIVGLGAQSLIVVI
                     GLTLGLIAGFYGGKTDNILMRIVDIVYSFPSYLLNIILVLVLGRGYFSIFVAIAATGW
                     AGMARLVRGQTLSTKSRDFVEAARASGAGNNRIMFRYILPNIIGPVIYSIFSGIPGVM
                     LVESGLSLIGLGVRPPMPSWGLMISEGTGQFLYNPYMLLAPAGMLVLTILSFNFVAFG
                     LRDALSARE"
     gene            2203..>2789
                     /locus_tag="P5310_05480"
     CDS             2203..>2789
                     /locus_tag="P5310_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019908032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRU32844.1"
                     /translation="MNLLLSIDNLNVHFYRSEGVLKAVDGISYNLYKGEVLGIVGESG
                     SGKSVSVLSIVRLLPANGKIVDGSISFDGKDMLEISRDELQDIRGKDIGFVFQDPMSS
                     LNPVITVGQQVIEPLLWHNLCSREEAYKRAKNMLGMVGIPSPEERMKEYPFQFSGGMR
                     QRVMIAMALICNPKLLIADEPTTSLDVTTQAQIIRL"
BASE COUNT          827 a          471 c          653 g          838 t
ORIGIN      
        1 gaaggcgtca gaacttgcca agaggataca taatataatc tacaccagat ccactccaga
       61 ggcaaggaaa atattaagtt tagttcaaca ggctcaaaga actacagatc cgaagaagag
      121 attagagcta tatagacagg ctaataagat aagagatata aatatgtata gttcattccc
      181 cttagttcaa ccaatgtcag caaggttggt taaatctaat tttgaggttc cattcaacaa
      241 gtatctggtc ggatttcctg taggctttgc atatattagg accaagtaaa aactgtttta
      301 agtatatcgg ggaagctata agcttccccg atatctggat agaaggagtg ataggatgac
      361 tcaatatata ataaagcgta tcggctggat aatctttaat ctattagtat taagtctcat
      421 agcttttatc cttatcaaaa atgtccctgg aagtttttta gaacttcaga tactgattgg
      481 tcaggcaggt acgatgggaa caggacttac aatttctgaa gtggaaaagg ttagaggggt
      541 agaaacagag atgatgaaaa ggtgggggga ggatgtccct ttatggcaac aatacctgac
      601 ctttataaag ggtttggtca ctctggatat gggaccatcc tttcgatggc ctacccaaac
      661 catagaggag ataatcgcca acaccttccc cgtatcattt acaatagcta ttctggctgt
      721 cactatagct attattatag gtatcccttt aggggtccta gctgcactta aacagaatag
      781 ctggatagat tatctggtat ctgcactcgt tatggttgtg agcgccatac ctaactatgt
      841 aatggcagta tttgtcatct tcttattcag tttctggatt ccaatattgc ctacttatgg
      901 atgggggcaa ccaaagaact atatccttcc tgtcctggct atgtcctttg gacccatagg
      961 aagtatagct ggctatatga gaacatctct attagatgct ttaaagcaag attatatcag
     1021 gaccgcctgg gctaaaggag gaacagaaag gcatgtgata tttggtcatg caatgaagaa
     1081 ctctttaata ccactggtta ctatccttgg tcctatgata agccacctaa tattggggac
     1141 agtctttgtt gagggtatgt ttggtgtccc gggtatggga cagtatttct cgcaggcggc
     1201 agggagccgg gattatccat tgatgatggc tcagacagtt atctttggtc tgatactgat
     1261 gataatgaac cttatagtag atataatata cttcttcctt gatccaagga tcagggtgtc
     1321 caggaggtaa atttatatgg gaaataaaaa gagaataggt agaagttact ggtctaatat
     1381 atgggaaaaa tttaaaagaa ataaattggc tgtatttggt ttgatctttg tctgtattct
     1441 tatactcctg ggtatatctg ctccttggtt agctccatac ccttacgatg aggttcatta
     1501 tgaagcggta tggactccac cctgtagaga atttttagca ggaacagacc agctggggag
     1561 agatatattt agtcgtctat tatatagcct aagaaatgct attatagtcg gtcttggtgc
     1621 gcaatcttta atcgttgtta tcgggttaac actggggctc atagctggat tctatggagg
     1681 aaaaacggat aatatactta tgagaatagt agatatagtt tactctttcc ccagctatct
     1741 tttaaatatt attctggtcc tggtattggg gagaggttat ttttctatat ttgtagctat
     1801 tgctgcaact ggatgggctg gtatggcaag actggtcaga gggcagacac tttctaccaa
     1861 gagtagagac tttgtagaag ccgcaagggc ttctggagca ggcaataata gaattatgtt
     1921 caggtatata ctgccgaata taattggacc agtaatatat tcaatattca gtggtatccc
     1981 cggagttatg ctggtcgagt ccgggttaag tctgataggt ctgggtgtaa gaccaccaat
     2041 gcccagctgg ggacttatga tatctgaggg aacgggacaa ttcttatata acccttatat
     2101 gttactcgcc cctgcaggta tgctggtttt aacaattctg agttttaact ttgtagcatt
     2161 tgggctgaga gatgctcttt ctgccagaga gtaggagggt atatgaattt actgttaagt
     2221 atagataatc tgaatgttca cttttatcga tcggaggggg tcttaaaagc agttgatggt
     2281 atctcttata acctgtacaa aggggaagtc ctgggaatag taggagagag tgggagtgga
     2341 aaaagtgtca gtgttctttc catagtgaga cttctacctg ccaatggaaa aatagtagat
     2401 ggctcaatct cttttgacgg aaaagatatg ctagaaatat ccagggatga acttcaggat
     2461 ataagaggaa aagatatagg gtttgtattt caggacccaa tgagttcatt aaatcccgtt
     2521 attaccgttg gacaacaggt tatagaacca ttactctggc ataacctgtg cagtagagaa
     2581 gaagcttata aaagagcaaa gaatatgctt ggtatggtag gtatcccctc acctgaggag
     2641 agaatgaagg agtatccatt tcagtttagt ggtggaatga gacagagggt tatgattgca
     2701 atggcactta tctgtaatcc aaaacttctg atagcagacg aacctaccac ttctctcgat
     2761 gttactactc aggctcagat aataaggct
//
LOCUS       DAOTKU010000205         2768 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_29733_length_2768_cov_3.776262, whole genome shotgun sequence.
ACCESSION   DAOTKU010000205 DAOTKU010000000
VERSION     DAOTKU010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29733_length_2768_cov_3.776262"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(211..1638)
                     /locus_tag="P5310_05485"
     CDS             complement(211..1638)
                     /locus_tag="P5310_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012582502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HRU32845.1"
                     /translation="MTQKITIQPVTRIEGHAKITIYVNDDGKVDNAYFHVDQFRGFEK
                     FSEGRPFYEMPIITPRICGICPVSHHLAAAKACDAIISAQIPRPAKLLRELMHMGQVI
                     QSHSMHFFELAGPDLILGWDADPKIRNVVGLIEANPELAIKAVKLRKFGQEVIAKLGG
                     RRIHPNYAVPGGVNNTLSIQDRDELLRDIDTMIPYIQEGVALAKDWIEKNRTMVEEFG
                     VFSTGYMGLVNEDGGLELYDGKVKLIGKDGNLLELFDRSEYLNYIGEHVEDWSYLKFP
                     FYKKLGWPEGVYRVGPLARLNVATKINTPLAQEEFKNFKSINGGKPVEGSLYYHYARL
                     IEDLYAAERAKELLLDQDILSDDILVTTDKILPEGVGVIEAPRGTLFHHYWVDERGSI
                     TRANLIVATGHNNWAMSKGVEEVAKRYVDGKNITEGMLNRVEGLIRCYDPCLSCSTHA
                     VGKMPMVIDIVSKSTGEVIKSIKRE"
     gene            complement(1651..2193)
                     /locus_tag="P5310_05490"
     CDS             complement(1651..2193)
                     /locus_tag="P5310_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012582501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP oxidoreductase"
                     /protein_id="HRU32846.1"
                     /translation="MKRITVATDWLDGCSGCHMSFLDMDERLVELIKMGVELNATPIT
                     DLKEPTEIVDIGILEGTVSTNHHIEVVKNMREKCKILIAMGDCAVFGGISTMRNYFSI
                     EELLRRGYIETESTVDGKIPTSPEIGKHIRFAKSVNQIVKVDFYIPGCPPRADAIYYM
                     LRELVEGRRPALIGDLLRYD"
     gene            complement(2206..>2768)
                     /locus_tag="P5310_05495"
     CDS             complement(2206..>2768)
                     /locus_tag="P5310_05495"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRU32847.1"
                     /translation="NFGVVGSYVKKRVFNGGELILVDDNSNSFGKYARAFYQTKDLDK
                     AIGSALMGNNPVVIYSKTEEGITDSLIRISGKAKFIWLTPSSNYLGAKKIGLNGSFVA
                     RKNAYIVGPCIEKLPEGLDRVIVQTSYYTPAINSADVVLPVSTWLEEEGKYINIEGRE
                     QKLVKVLNPPKEVKGNREIIDILLAR"
BASE COUNT          719 a          665 c          430 g          954 t
ORIGIN      
        1 tctttgattt cataatcatt ttcctggtca attccagcat caataaaaaa aacaagctcg
       61 tatcctgcaa tatcttctga aatttcagga ccgagctcct gaacattgat aacatccaca
      121 acttctaaat cttcattcag tctctctgtt atatattctc caataccatc atcctgtctg
      181 gcagggtttc catatcctat aacgaggatt ttattctctt ttgatacttt taatcacctc
      241 tccggtggat ttagaaacta tatctataac catcggcatc ttgcccactg catgtgtgga
      301 acaggagaga caggggtcat aacatcttat caatccttct accctattga gcataccttc
      361 agtaatgttc tttccatcca cgtatctctt ggctacttcc tctacacctt tactcatagc
      421 ccaattgtta tgcccggtag caactattag gttagccctg gtaatgcttc ccctttcatc
      481 tacccagtag tgatggaata gtgtacccct tggtgcctct attacgccaa caccctctgg
      541 aagtatcttg tcggtagtta ccaggatgtc atctgataat atatcctggt ccagtaagag
      601 ttcttttgcc ctttcggctg catagaggtc ttctataagt ctggcataat gataatacag
      661 tgaaccctct actggcttac ctccgtttat actcttgaag tttttaaatt cttcctgagc
      721 aagaggagta ttaatctttg ttgccacatt taatcttgcc agaggtccga ccctgtatac
      781 accttctggc catccaagtt ttttgtaaaa tgggaacttc agataagacc agtcttccac
      841 atgttcccct atatagttta aatattctga cctatcgaat aattctaaca gatttccatc
      901 cttaccaatc agttttactt taccgtcgta caattccaat ccaccatctt catttacaag
      961 tcccatatat ccggtagaga atacaccaaa ttcttctacc attgtcctgt tcttctctat
     1021 ccagtcctta gccagagcta caccttcctg aatatagggg atcatagtat ctatatctct
     1081 gagcagttca tccctatcct ggatagataa tgtattattt acccctccag gaacagcata
     1141 gtttggatgt attcttcttc ctcccagttt tgctatcact tcttgcccaa attttctcaa
     1201 tttgacagcc tttattgcta attctgggtt agcctctata agtccaacta catttcttat
     1261 cttggggtct gcatcccagc ccagtattaa atctggtccg gcgagttcaa agaagtgcat
     1321 ggaatgcgac tgtatgacct gtcccatatg cataagctct ctgagaagtt ttgccggtct
     1381 gggtatctgt gcagatataa tagcatcaca agccttggct gctgccagat gatgacttac
     1441 aggacatatt ccacatatac ggggagttat gattggcatc tcatagaagg gtcttccttc
     1501 actgaacttt tcaaatcctc tgaactggtc tacatgaaaa tatgcattat ctacctttcc
     1561 atcatcgttt acatatatgg ttatctttgc atgaccctct attctggtta ctggttgaat
     1621 tgttatcttt tgagtcattc ctgcctcctt ttaatcatac cttaataaat cgccaataag
     1681 agcaggtctg cgtccttcta ctaattctct cagcatataa tatattgcgt ctgctctggg
     1741 tggacatcca ggtatataaa aatctacttt tactatctgg tttacagatt ttgcaaaacg
     1801 tatatgttta cctatctctg gagatgtcgg aatctttcca tcaacagtac tctctgtctc
     1861 aatatatcca cgcctcaata actcttctat agagaaatag tttctcatag ttgagatacc
     1921 accaaatact gcacagtctc ccatagctat taaaatctta cacttctctc tcatattctt
     1981 tacaacttct atatgatggt tggtactgac agttccttcc aggatgccta tatcaactat
     2041 ttctgtgggt tcttttaagt ctgtaattgg agtagcattc aactctaccc ccatctttat
     2101 aagctcgact aatctttcat ccatatccag gaaagacata tggcaaccag agcatccatc
     2161 gagccaatct gttgctacag taattctctt cataatgtcc tccttttatc ttgccagaag
     2221 tatatctata atctccctat tcccttttac ctctttaggg ggattcaata cctttacgag
     2281 tttttgctct cttccttcaa tatttatgta tttcccttcc tcttctaacc aggtagatac
     2341 aggtaatacc acatcagctg aatttatagc tggtgtgtaa taactggtct gaactataac
     2401 cctatctaga ccttctggaa gtttctctat gcaaggacca actatatagg cattctttct
     2461 agcaacgaag ctgccattaa gacctatctt tttggctcca agatagttag aggatggggt
     2521 tagccatata aattttgcct ttccgctgat gcgtattaag ctatcagtta taccctcttc
     2581 tgttttgctg tatatgacta cagggttatt tcccatgagt gcactaccta tagccttatc
     2641 taggtctttt gtctgataga atgccctggc atatttacca aaactattgg aattatcatc
     2701 taccaggatg agttctccac cattaaagac cctctttttt acataggaac ctacaacccc
     2761 aaaatttt
//
LOCUS       DAOTKU010000206         2761 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_29828_length_2761_cov_2.928677, whole genome shotgun sequence.
ACCESSION   DAOTKU010000206 DAOTKU010000000
VERSION     DAOTKU010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29828_length_2761_cov_2.928677"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1067
                     /locus_tag="P5310_05500"
     CDS             <1..1067
                     /locus_tag="P5310_05500"
                     /inference="COORDINATES: protein motif:HMM:NF013747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRU32848.1"
                     /translation="EKFIGMIGELRGNSTMINCWIALLRRDDYSVQSKAINALIKIGK
                     PAVKPLISNLNDKDNSFRMAAIETLGKIKDPAAVGPIINLLKDEDSSVRMRAAEALGN
                     IGDKRAVEPLIALIKDEDSWVRMAVVEALGKIKDPRVIEPLIAALKDEDSWVKMKAIE
                     ALRDIKDQRALEPLIASIKDSNASVRTRAAEALGALKNPKAVPALIERLKDKEADVRM
                     RSAEALGNIGDKRAVKPLIASLKDEDWTVRLRSAEALGKIGDSNAVKPLIEALNDKEW
                     TVRSSAAEALGKIKDTRAIDPLIKVLADKDIAVRLSVIEALGEIGDKKAIEPLKKVAS
                     NDSSPIVKEKAKEILNILQR"
     gene            complement(1079..2218)
                     /locus_tag="P5310_05505"
     CDS             complement(1079..2218)
                     /locus_tag="P5310_05505"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRU32849.1"
                     /translation="MKRNSGFTILALSVFLLNLSMGLHTSVFNNYAAQELNLHPEELG
                     ILESFRETPGLLIAFISAFLGFLSKQYIAMLSFILASIGFSFYSKVSNLSGLIYAGVT
                     WSLGIHLWMTLSPIFTLSFAEGGERGRRLGQINSITAFSSLTAMVLVFLTGNFIPFRK
                     YYLIAGILPIIGAFLVLKVPYKSRVDVDPPSLVFKKDYMLYYVLNFLEGSRRQVFATF
                     ALFVLTRVYGVPIRHITLLLIINGIANILVAPRAGKLIDRVGERIVLLLSYTGALLIF
                     IGYATTRSVTVLSILYCLDNIMINFSLALTTYIDRISILGDLSPNISMGVTVNHIAAV
                     LMPLVGGILWERFGYQTMFMLGALIVLISIFFANKIKPREESITV"
     gene            complement(2219..>2761)
                     /locus_tag="P5310_05510"
     CDS             complement(2219..>2761)
                     /locus_tag="P5310_05510"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRU32850.1"
                     /translation="EVIFMKRKLPCTSILGAISSALGLWLLTGLYNFNMNYGDFLVLI
                     CALCFASQIIVVGICSPNSNTGVISTTQVIVVAILSWISIPIFGERLNTPISANAIWA
                     IIITAIFATALAFYIQALAQRSINPTTVALIFATEPVFGGIFGYLLLGEILSPSALFG
                     AGLILLGMILTQIPERRRKE"
BASE COUNT          977 a          523 c          584 g          677 t
ORIGIN      
        1 acgaaaaatt cataggtatg ataggagagc tccgaggtaa ttcaacaatg ataaactgct
       61 ggatcgcatt actaagaagg gacgattatt ctgtccagtc taaagcgatc aatgccctta
      121 taaagatagg gaagccggca gtaaaacctc ttataagtaa tcttaacgat aaagataatt
      181 ctttcaggat ggctgccatt gagactcttg gaaaaataaa agaccctgca gctgtaggac
      241 cgattataaa tctactcaag gacgaggaca gttctgtcag gatgagagca gcagaagcat
      301 tgggcaatat cggagataaa agggctgtag aacctctcat agctcttatt aaagatgagg
      361 atagctgggt tagaatggct gtagtagagg ctttagggaa gataaaagac cctagagtaa
      421 tagaaccact tatagctgcc cttaaggatg aggatagctg ggtcaagatg aaggctatag
      481 aagcattaag agatattaag gaccagagag cgctagaacc ccttatagct agcattaaag
      541 atagtaatgc ctctgtaaga acgagggcgg cggaggcgct aggagcatta aagaacccaa
      601 aagcggtacc cgcccttata gaaaggctca aagataaaga agctgatgtc aggatgagat
      661 cggcagaggc attgggtaat attggagata aaagggctgt aaaacctctt atagccagtt
      721 taaaagatga ggattggaca gtgagattga gatcagcaga ggcgttaggt aagattggag
      781 actcaaatgc ggtaaaaccg cttatcgaag ctcttaacga taaagaatgg acagtcagat
      841 caagcgctgc agaagctctt ggaaaaataa aagacaccag ggcaatagac cctcttataa
      901 aagtccttgc tgataaggat atcgctgtta gattaagcgt aatagaggca ttgggagaaa
      961 taggagataa aaaggctatc gaaccattga aaaaagtagc cagtaatgac tcttctccga
     1021 tagtaaaaga aaaggcaaaa gagatattaa atatcttaca gagatagctc cttaaaaact
     1081 ataccgtaat actttcttcc ctgggcttaa tcttattggc aaaaaatata gagattaaga
     1141 ctattaatgc ccccagcata aacatagttt gatagccaaa tctctcccac agaatccctc
     1201 caacaagagg cataagaaca gccgctatat gattaacagt aacacccata gagatattgg
     1261 gagataaatc acccagaata gaaatcctat ctatataggt agtaagagcc agactaaaat
     1321 taatcataat gttatctaaa cagtaaagta tagagagtac tgttaccgat ctggtagtgg
     1381 catagccgat aaatataagt aacgctccgg tgtaacttaa cagcaggaca atcctctccc
     1441 ccaccctatc tatgagctta cctgctctgg gggctacaag tatattcgct attccattta
     1501 ttataagtaa tagagtaatg tgtcgtattg gaactccgta tactctggtc aaaacaaaaa
     1561 gggcaaaggt ggcaaagacc tgccttctgg aaccctccag gaaattcaat acataataga
     1621 gcatatagtc tttcttaaag accagagagg gtgggtctac atcaaccctg gacttatatg
     1681 gaacttttaa taccagaaat gctccaataa ttggaaggat acctgctatc agatagtatt
     1741 tcctgaatgg tataaaatta cctgtgagaa atacaaggac catagcggta agacttgaaa
     1801 atgcagttat agagttaatc tgtccaagtc ttcttcctct ttctcctcct tctgcgaagc
     1861 tcaaagtgaa gataggactt aatgtcatcc aaagatgtat ccccagactc caagtaactc
     1921 cagcgtatat aagtccagaa agattactga ctttagaata aaagctgaac cctatactgg
     1981 caaggataaa agagagcata gcaatatatt gtttacttaa aaaacctaaa aaggcactta
     2041 taaaagcgat aagcaatccc ggggtctctc tgaatgactc taaaatacct aattcttcgg
     2101 ggtgtaaatt taactcctgg gctgcgtaat tgttaaatac agaggtatgt aaccccatac
     2161 tcaaatttaa aagaaataca gacagagcaa gtatagtaaa tccagaattt ctcttcatct
     2221 actccttcct tctcctttcc ggaatctggg taagtatcat tcccaataat atcaaacctg
     2281 cgccaaagag tgctgacgga ctcaaaatct ctcccagcag tagataacca aatataccac
     2341 caaagaccgg ttcagtggcg aagataagcg ccacagtggt agggttaata ctcctctgtg
     2401 ctaatgcctg tatatagaag gctaaagcag tggcaaatat agcggtgatt ataatcgccc
     2461 atatggcatt agcagagata ggggtattta acctttctcc aaagataggg atagatatcc
     2521 agctgagaat agccacaact ataacctgag ttgtagagat tacaccggta ttactgttag
     2581 gagaacatat cccaaccact attatttgag aggcaaaaca tagtgcacag ataagtacta
     2641 agaagtcacc atagttcatg ttaaaattat ataatccggt gagaagccag agaccgagag
     2701 cagaagatat agcgccaagt atagatgtgc agggaagctt cctcttcata aaaatgacct
     2761 c
//
LOCUS       DAOTKU010000207         2758 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_29871_length_2758_cov_3.016648, whole genome shotgun sequence.
ACCESSION   DAOTKU010000207 DAOTKU010000000
VERSION     DAOTKU010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29871_length_2758_cov_3.016648"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="P5310_05515"
     CDS             <1..586
                     /locus_tag="P5310_05515"
                     /inference="COORDINATES: protein motif:HMM:NF028352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotide-binding domain containing protein"
                     /protein_id="HRU32851.1"
                     /translation="IKITAGCAGFARTLANLIYPEKRDRSIHLELKARPLILVCGSIN
                     EVSLRQVEYAKRNYQALDIVLSLEDIINGTIDLPDINGEEIILIRTTDKHLDINSYKD
                     LPVIIQERLGEITSRFLQLTKSNTLVVFGGDTLLGITEKLGISLLYPLIEIEDGVVLS
                     EASSFFFITKAGGFGKEDIIDRIISFIRGHLPEV"
     gene            complement(558..1325)
                     /locus_tag="P5310_05520"
     CDS             complement(558..1325)
                     /locus_tag="P5310_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018153219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRU32852.1"
                     /translation="MILKVDGIEFSYNSHPVLSNISFEVNRGEILSILGNNGAGKTTL
                     LKCISRILKPVRGVVFVDGKSLSDISATEIARKISYVPQRQDVSRFTVFDTVLLGRKP
                     YIRWDVSKKDLLIVEEILDRLGLKTLALKYLDQLSGGELQKVSIARALAQDPEILLLD
                     EPTNNLDLKNQIEAIRIIRDITRSGNIASIVVIHDLNLAMRFSDKFLLLKENTIFAYG
                     DMEVMTPENISSVYGVKVTVEKINGIPVVIPLEDVLL"
     gene            complement(1322..2377)
                     /locus_tag="P5310_05525"
     CDS             complement(1322..2377)
                     /locus_tag="P5310_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRU32853.1"
                     /translation="MLNTWEKYLEYTRTKIIIIIIISLLIFLISIYAINAGSSGLSVR
                     EVLMTLLGRGDNRTNIIVWNIRLPRVLSAIVAGIGLSIAGCVTQTILRNPLASPFTLG
                     ISQGAAFGVALAVTILGTDAIHSTSIDAAMVNNPYLTTLCAFIGAMSATMIIMALSKR
                     GRVSPESMVLAGVAISSLFSAMTTVVQYFADELDVSAMVFWTFGDMGRANWRDLKIMT
                     LVVLLSGIYFLINRWNYNALAGGEEMAKSLGVPVERLRVISMFIASLVTSVIVSFLGV
                     IGFICLVGPHIARRLVGSDHRFLISGSIAIGGVLLLLADTFARTIIAPVVLPVGAVTS
                     FIGAPLFLYLLTKGYKR"
     gene            complement(2381..>2758)
                     /locus_tag="P5310_05530"
     CDS             complement(2381..>2758)
                     /locus_tag="P5310_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HRU32854.1"
                     /translation="TGREGSIMIEKEKLLIWDPDIIFIDLGNYNLVKQDYHKNPQFYQ
                     ALKAVKNGNVYGQLPFNFYNTNIDTAIVDAYWAGKVIFPEQFRDIDPVKKANEIYRFF
                     LGKSLYEELTRTYGSLTGINIGE"
BASE COUNT          851 a          618 c          506 g          783 t
ORIGIN      
        1 gatcaaaatc actgccggtt gtgctggctt tgcaagaaca cttgccaatc taatatatcc
       61 agagaaaaga gacaggtcca tacatttaga gctgaaagcc agacctctta tactggtgtg
      121 tggaagtata aatgaggtct cactcagaca ggtagagtat gcgaaaagaa attatcaggc
      181 gcttgatata gtgctatcac ttgaggatat aataaatgga actatagacc tgcctgatat
      241 aaatggggag gagattattc taataagaac tacagataag catcttgata taaactcata
      301 taaagatcta ccagttataa tacaggaaag acttggggaa attacttcaa ggttccttca
      361 actaacaaaa tccaatactc tggtagtctt tggaggagac accctcctcg gaataactga
      421 aaaattagga ataagtctcc tctatcctct tatagagata gaggatggag tcgttctatc
      481 ggaagccagt tcgttcttct tcatcacaaa ggcaggtggc tttggaaaag aagatataat
      541 cgacaggata atctctttta taagaggaca tcttccagag gtataaccac agggatacca
      601 tttatcttct ccacagttac tttaactcca tatacagagg agatattctc aggtgtcata
      661 acttccatat ctccatatgc aaatatggtg ttttctttca gtaggagaaa tttatcggaa
      721 aatctcatgg caagattcag gtcatgaata acaactatac tggcaatgtt accgctcctg
      781 gttatatccc tgatgatacg gatagcctca atctgatttt tcaggtccag gttattcgtg
      841 ggctcatcca ggagaagaat ctccggatcc tgagccaggg ctctggctat agagactttc
      901 tgtaattcgc caccactcaa ctggtccagg tatttcagcg caagggtttt caatccaaga
      961 cggtccagaa tctcttccac aataagaagg tcttttttag agacatccca cctgatataa
     1021 ggctttcttc caagcagcac cgtatcaaat acggtaaatc tggaaacatc ctgtctctga
     1081 gggacatagc ttatcttccg ggctatttcg gtagcagaaa tatcggataa gctctttcca
     1141 tcaacaaaaa ccacacctct aactggtttc agtatcctgc ttatacactt cagcagtgtt
     1201 gtctttcctg ccccgttatt tcccagaata gagagaattt ctcccctatt gacctcaaaa
     1261 ctgatattac ttaacactgg gtgactatta tagctgaact ctattccatc aaccttcaat
     1321 atcatctctt gtaacctttt gtgagtaaat ataaaaagag tggagcacct ataaaagatg
     1381 taactgctcc tacgggtaat accaccggtg caataatcgt cctggcaaat gtatctgcca
     1441 gtagaagcag gactccacct atagctatag aaccagagat tagaaatcta tgatcactac
     1501 cgactaacct tctcgctata tgtggtccta ccaggcatat aaaacctatt actcctaaaa
     1561 aagagactat taccgaagta accagagagg ctataaacat acttataact cttaacctct
     1621 ccacaggcac acccagactt tttgccatct cttctccacc agccagtgca ttataattcc
     1681 atctatttat aagaaaatat atccctgaga ggaggacaac cagtgtcata atcttcaggt
     1741 ctctccaatt tgccctcccc atatccccaa acgtccagaa gaccatagca gaaacatcca
     1801 gttcatcagc aaagtactgc accacagtag tcatagcaga aaagagagaa cttatcgcca
     1861 ctccagccag gaccatagac tcaggagaaa ctcttccacg tttagagaga gccattatta
     1921 tcatcgttgc actcatagct ccgatgaatg cgcagagcgt ggttaaataa gggttattca
     1981 ccattgcagc atctatagaa gtgctgtgaa tagcatcagt ccccaaaata gtaactgcta
     2041 gggcaacacc aaaggctgcc ccctgagata tcccgagggt aaatggagat gccagaggat
     2101 ttctgagtat agtctgagtg acacatccgg caatggatag cccgatcccg gctacaatag
     2161 ctgataatac tcttggaagt cttatattcc atacgataat atttgttcta ttatctcccc
     2221 tgcccagaag tgtcataagt acctctctta cagaaagacc ggaagaacct gcgtttatag
     2281 cataaatact tattaggaat atcaatagag agattattat aataataatg atcttcgtcc
     2341 tggtatactc cagatatttc tcccaggtat ttaacatctc ttattcacct atattgatac
     2401 cggtaagact accataagtc ctggtcagct cttcgtataa agatttgccc aggaaaaacc
     2461 tgtaaatctc gttggctttt tttacagggt ctatgtccct aaattgctcc gggaagatga
     2521 ccttacctgc ccagtaggca tcaactatag cggtgtcgat atttgtattg tagaaattaa
     2581 atggtagttg tccatatacg ttcccatttt ttaccgcctt cagcgcttga tagaactgag
     2641 gattcttatg ataatcctgt tttaccagat tataattacc aaggtcaata aatattatat
     2701 cagggtccca gattagaagt ttttcttttt caatcataat actcccctcc ctgcctgt
//
LOCUS       DAOTKU010000208         2740 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_30116_length_2740_cov_3.755307, whole genome shotgun sequence.
ACCESSION   DAOTKU010000208 DAOTKU010000000
VERSION     DAOTKU010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30116_length_2740_cov_3.755307"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /locus_tag="P5310_05535"
     CDS             <1..705
                     /locus_tag="P5310_05535"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HRU32855.1"
                     /translation="NRVVGVSTYCNYPEEAKTREKIGTFQDPNIEKVLALKPDLVIST
                     GGVQRQFIERLEKMGIPVYTSYPHNLDEVLKSIYKIGLVTGAEKEANDLIFDLKFRIA
                     RVTSKVGKVKNKPKVFFELWHEPLMSAGPGSFIDDLIKKAGGINLAGDAKTAYPIFSL
                     EQLIKEDPDVIIAAESSMGGSLSDIAKRPGWGMLKAVKNQKIFTINDDIIFRAGPRLV
                     IALETIAKYLHPELFK"
     gene            702..1220
                     /gene="cobU"
                     /locus_tag="P5310_05540"
     CDS             702..1220
                     /gene="cobU"
                     /locus_tag="P5310_05540"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: protein motif:HMM:NF014351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HRU32856.1"
                     /translation="MSLTLILGGARSGKSKFAQKLCENKEAVVLIATALPLDDEMEER
                     IKRHRAERSPHWKTVEEPEDIFNAIRNSSYEDYIIVDCLTMWVSNLLEKYTDEEVARI
                     AEEISAYSRGLKGEVIVISNEVGLGIVPEYPLGRRYRDLLGRVNQIFAGNANKVLFMI
                     AGIPMEVKNESK"
     gene            1207..2265
                     /gene="cobT"
                     /locus_tag="P5310_05545"
     CDS             1207..2265
                     /gene="cobT"
                     /locus_tag="P5310_05545"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HRU32857.1"
                     /translation="MNLNEAISNIKPLDRHVQERVWSRWDSLTKPRRSLGRLEEIVVQ
                     LAGITGSTTPEIEERYIVVMAGDHGVAISGVSAYPQEVTVQMVHNFLSGGAGINAIAS
                     VARAKVLVVDVGVAEKIETTSPYFRDYNVARGTEDFSKGPAMSREAAIGSIEVGIKVA
                     EELNNSGFDIAGTGEMGIGNTTPSSAILSVFSPSTSLDILIGPGTGLDGEGIRRKREL
                     IERAIEINRPDPNDPIDVLAKVGGLEIGAIAGFIIGCARVRKPVMIDGFISTVGALLA
                     YRIAPGVLPYMIPSHLSQEPGHKIALEMMGLKPLLTLDMRLGEGTGSAIGMLIIDCAL
                     SAYYNMKTFEEAGVSRNA"
     gene            2258..>2740
                     /locus_tag="P5310_05550"
     CDS             2258..>2740
                     /locus_tag="P5310_05550"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:NF014685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HRU32858.1"
                     /translation="MLEFIEALRFLTILPLRAPFNESALRKSPRVFPLAGLFIGIVTS
                     ISFLIISRISTREIASLITLFVWEVITGGIHLDGFADTLDGIMSRRDREGILEIMRDS
                     RIVTFGATGIFFMLGLKYLAISNSGVPVYTLLLSPVIGRFLITLSIYLFPYARITGKG
                     S"
BASE COUNT          840 a          474 c          692 g          734 t
ORIGIN      
        1 aacagggttg ttggcgtaag cacatactgt aactatccag aagaggctaa aaccagagaa
       61 aaaatcggta cttttcagga cccgaatata gaaaaagtcc tagcattaaa acctgacctg
      121 gtaatttcaa caggtggtgt gcagagacaa tttatagaga gattagagaa gatgggtatt
      181 cctgtatata cgtcataccc gcataatctg gatgaggtat taaagagtat ctataaaatt
      241 ggacttgtaa ctggagcaga gaaagaggct aatgacctga tatttgattt gaaatttaga
      301 atagccaggg ttacaagtaa ggtcggtaag gtaaaaaata aacccaaggt cttctttgaa
      361 ctctggcatg agccacttat gagcgctggt ccaggaagtt ttatagatga tttaataaaa
      421 aaagctggag ggataaacct ggctggggat gccaagaccg catatccaat atttagcctt
      481 gaacaactta ttaaggaaga ccctgatgtc ataatcgctg cagaatccag tatggggggc
      541 agcctttcgg atatagcgaa acgccctgga tgggggatgc tgaaggctgt aaagaatcaa
      601 aagatattca caataaatga tgacataatc tttagagcag gtcccagact ggttatagcg
      661 ctagaaacta tagctaaata tctccatccg gagctcttta aatgagttta acacttatac
      721 ttggtggagc cagaagcggt aagagtaagt ttgcccagaa actctgtgag aataaagagg
      781 cggtagttct tatagctacc gccctgcctc tagatgatga gatggaagaa cggataaaaa
      841 ggcacagggc agagaggtca ccacactgga agacggtaga agagcctgaa gacattttta
      901 atgccataag aaactcctct tatgaagatt atatcatagt tgattgtctt acaatgtggg
      961 tgtcgaatct tttagagaaa tataccgatg aagaggtggc aagaatagct gaagaaatct
     1021 ccgcttactc tagggggctt aagggtgagg taattgtcat atccaacgag gttggactcg
     1081 gtatcgtccc tgagtatcct cttggtagaa ggtacaggga cctgcttggt agagtaaatc
     1141 aaatatttgc aggtaatgct aataaggtac tttttatgat tgcagggata cctatggagg
     1201 taaagaatga atctaaatga agctatttct aatataaaac ctctggatag acatgtgcag
     1261 gaaagggtct ggtccaggtg ggatagtctt acaaaacccc gtagaagtct tggaagattg
     1321 gaagagattg tggtacagct tgcgggtatc accggtagca ccacccctga gatagaagag
     1381 agatacattg tggttatggc tggagaccat ggtgtagcca taagtggtgt aagtgcatac
     1441 ccgcaggagg taactgtaca gatggtacat aactttttat caggtggagc ggggataaat
     1501 gcaatcgcct ctgtagccag ggcaaaggta ttagttgttg atgttggagt tgcagagaag
     1561 atagagacca cctctccata ttttagagat tataatgtag ccagaggcac tgaggatttt
     1621 tctaaaggac ctgctatgag cagagaggct gctataggtt caatagaggt agggataaag
     1681 gtagcagaag agctgaataa ttctggtttt gatatagcag gaaccggcga gatgggtata
     1741 ggtaacacta caccgagcag tgcaatactc agcgtatttt caccatcaac atccctggat
     1801 atactgatcg gtccaggtac aggtctggat ggagagggga taagaagaaa gagagaattg
     1861 atagaaaggg ctatagagat aaacagacct gatccgaatg acccaataga tgtcctagca
     1921 aaagttggtg gattggagat cggagcaata gctggattta taataggctg tgccagagta
     1981 agaaagccgg tgatgattga tggatttatc tcaacggtag gggcattgct tgcatacagg
     2041 atagcccctg gtgttctccc atatatgatt ccctctcatc tatctcagga acctggtcat
     2101 aagatagcac tggaaatgat gggtctcaag cctctactta cactggatat gagattggga
     2161 gaagggacag gttctgctat aggtatgctt attatcgact gtgcactcag cgcctactat
     2221 aatatgaaga catttgaaga ggctggtgtc tcaagaaatg cttgagttta tcgaggctct
     2281 cagattttta accatattac ctctaagagc cccctttaat gaatctgctt taaggaaaag
     2341 tccaagggtc tttccactgg ctggtctttt tataggaatt gtcacttcta tatcttttct
     2401 tatcatatcc aggatatcta ccagagagat tgcctccctc ataactcttt tcgtctggga
     2461 ggttataaca ggtggcattc acctggatgg ttttgctgat actttggatg gtattatgag
     2521 cagaagagat agagagggta tcctggagat aatgagagac agcaggatag tgacatttgg
     2581 agcgacaggg atattcttta tgttgggatt aaagtatctt gccatcagta attcaggtgt
     2641 ccccgtctat acccttttac tctctccagt aatcggcaga tttttgatta ccctctccat
     2701 atacctgttt ccatatgcta ggataacagg taaggggagt
//
LOCUS       DAOTKU010000209         2740 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_30122_length_2740_cov_2.707263, whole genome shotgun sequence.
ACCESSION   DAOTKU010000209 DAOTKU010000000
VERSION     DAOTKU010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30122_length_2740_cov_2.707263"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            66..1073
                     /gene="secF"
                     /locus_tag="P5310_05555"
     CDS             66..1073
                     /gene="secF"
                     /locus_tag="P5310_05555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HRU32859.1"
                     /translation="MGYFEDSKINIIKHSSIWLGVAVLVIISTVLLFFTRGLNFGLDF
                     TGGTLIEYKLGKSFDNVGEIRPLLAELNVESSFIQKTDENGVIIRVKELTNEQRDAIT
                     KKLSDNFDSAELIRQEFIGPVIGQELRRTAIIATILGLLAQVIYIGVRFKFDMALAAD
                     VALLHDICLVVLVFIITQKEVNTPFIAILLTIVGYSINDTVVIFDRIRENLKLKPKDS
                     RETFPQLVNRSLLETMTRSINTVLTTVIAVLAVYLFGGTAIRDFAFGLMIGIATGGYS
                     SIFIAAPVLVLMRKKEWTREMENIKETPVTPAAPTSRATDAGQPAAPKGKVSTTKRKR
                     R"
     gene            1079..1396
                     /locus_tag="P5310_05560"
     CDS             1079..1396
                     /locus_tag="P5310_05560"
                     /inference="COORDINATES: protein motif:HMM:NF018058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HRU32860.1"
                     /translation="MNYVYLVIVFIVAFVIALFVQINNYPVNLNYFFGTTEISFALAL
                     FLSFVVGFLLAVIARIPQDLVNRRKVRNLEREIIDKENRLRNLYTSQSQSPQGEDKGE
                     SKT"
     gene            1406..>2740
                     /gene="recJ"
                     /locus_tag="P5310_05565"
     CDS             1406..>2740
                     /gene="recJ"
                     /locus_tag="P5310_05565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HRU32861.1"
                     /translation="MQFVLNDSPEWETLYRGLGVLPSIAKVLVRRGISSPVSAERFIS
                     SQVKDVSNPFLLPDIEPALSRIKMASIDKEKICLYGDYDVDGLTSVAVVLHTMKGLGF
                     DVSYYIPNRLSEGYGLNINAIDRIKEGGSSLIITLDCGVSSVSAIDYAKSSGIDVIVV
                     DHHNPPATLPPAIAIVDPKKEDSIYPFKDLSGVGVAWQFARAIINSLLEEDTYLEETL
                     DIVALGTIADVVPLLDENRVIVRRGLSRLKFSPRVGLSALMKRAGIEGRMMNAELLAM
                     TLIPRLNSAGRLGSAELAMDLLLTVDPVNARLISERLEENNQKRRGLVDEVLDEVLKR
                     LEDKVIASTILEYSETWHSGVLGIVASRLVDMFNRPVFIGKKENDTVKFSVRSPEEID
                     IYSILAGLKDTFLKFGGHRYALGFTIRESEIGRLLDLLEESIKIGDHEELVEI"
BASE COUNT          805 a          460 c          659 g          816 t
ORIGIN      
        1 ttcaactgtt cagtcagctg aagattatga agagccctaa gacgtttggg atataggagg
       61 aacttatggg atactttgaa gattctaaga taaacataat aaagcactct tctatatggt
      121 taggtgtagc tgttttagtc atcatctcta cagtactgtt attctttacc agaggactta
      181 attttggtct tgatttcacc ggtgggactc tgatagagta taaactcggt aaaagttttg
      241 ataatgttgg agagataaga ccgttactgg cagagttaaa tgtagaatct tcctttatcc
      301 agaaaacaga tgaaaatggt gtaataataa gggtaaagga gcttacaaat gaacagagag
      361 atgctataac taaaaagcta tcggataact ttgacagtgc tgagctgata agacaggaat
      421 ttataggtcc agttataggg caggaactga gaagaactgc catcatagct accatacttg
      481 gactattggc tcaggttatt tatataggcg taaggtttaa gtttgatatg gctcttgcag
      541 cagacgttgc tctccttcat gatatatgtc tggttgtact tgtatttatc attacccaga
      601 aagaagttaa taccccattt atagccatac tgcttacaat agtcggttac tctataaacg
      661 atacggttgt tatcttcgac aggataaggg aaaatctaaa gttaaagcct aaagattcca
      721 gggagacttt ccctcagctg gtaaatagaa gtctattaga aacaatgacc aggagcataa
      781 atactgttct tacaactgta atagcggtcc tggcggttta tctgtttggt ggaactgcta
      841 taagggattt cgcctttggg cttatgattg gaatagccac aggaggttac tcgtctatat
      901 ttattgcagc tccagtatta gtacttatga gaaagaagga atggactaga gagatggaaa
      961 atattaagga gactcctgtt actcctgctg cccctacttc cagggcaaca gatgcaggtc
     1021 agcctgcagc ccccaaagga aaggtgtcca caaccaaaag gaagaggagg tgaaatgggt
     1081 gaattacgta tatctggtta tagtatttat agttgccttt gttatagctc tttttgtcca
     1141 gattaataat tatcctgtta atctgaatta tttctttggc accacagaga taagttttgc
     1201 cctggctctt ttcctctctt ttgtagtggg gtttctcctt gcggttatag caaggatacc
     1261 tcaggacctt gtaaatagaa gaaaggttag aaatttagag agagaaatta tagataaaga
     1321 gaataggttg agaaaccttt atacctctca gagtcagagc ccacaaggag aggataaggg
     1381 agagagtaaa acataatatc tctaaatgca gtttgtcctt aatgatagtc cagagtggga
     1441 gaccctttat agaggtctgg gtgttctccc atccatagct aaggttttgg tcaggagagg
     1501 aatttcctct cctgtttctg ctgaaagatt tatttccagc caggttaaag acgtctctaa
     1561 ccctttttta ctccctgata tagagccagc tctctcgagg ataaagatgg cttctattga
     1621 taaggagaag atatgtctct atggagatta cgatgttgat gggcttactt cagtggcggt
     1681 ggtgcttcat acaatgaagg gtcttggctt tgatgtatct tattatattc ccaacaggct
     1741 ttctgaaggc tatggtctca atataaatgc gatagacagg ataaaagaag gtggatcttc
     1801 tcttattata actctagact gtggtgtgtc ttctgtctca gcaatagatt atgccaagtc
     1861 atcagggata gatgtgatag ttgtcgatca tcataatcca cctgcaactt tacctccggc
     1921 tatcgctatt gttgatccaa agaaggagga ttccatatac ccatttaagg acctctccgg
     1981 tgttggggtt gcctggcagt ttgccagggc tattataaac agtcttttag aagaggatac
     2041 atatctggag gagactctgg acattgtcgc tttaggaaca attgctgatg tggtcccatt
     2101 actcgatgag aatagagtca tagtaaggag agggctctcg agattaaaat tttctccaag
     2161 ggttggactt tctgccctga tgaaaagggc gggcatagag ggcaggatga tgaatgctga
     2221 acttttagcc atgacattaa tcccccggtt gaactctgcc ggaagattgg ggtcagcaga
     2281 acttgcaatg gaccttttac tcacagtgga ccctgtaaac gccaggttga tatctgagag
     2341 attggaggag aataatcaga agaggagagg tcttgttgat gaggtcctgg atgaggtttt
     2401 gaaaaggctt gaggataaag tcattgcatc tacaatactg gaatattctg aaacgtggca
     2461 ttccggtgtc ctcgggattg tggcatctag actggttgat atgtttaaca gacctgtttt
     2521 tataggtaag aaagagaatg atacagttaa gttctcggtg agaagcccgg aggaaatcga
     2581 tatatattcg atacttgcag gtctgaaaga tactttttta aagtttggag gacaccgtta
     2641 cgcattgggt tttactataa gagaatcgga gatagggaga ttactggacc tgctggaaga
     2701 gagtataaag ataggtgatc atgaagaact ggtggagatc
//
LOCUS       DAOTKU010000210         2729 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_30295_length_2729_cov_3.023186, whole genome shotgun sequence.
ACCESSION   DAOTKU010000210 DAOTKU010000000
VERSION     DAOTKU010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30295_length_2729_cov_3.023186"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..903
                     /locus_tag="P5310_05570"
     CDS             <1..903
                     /locus_tag="P5310_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32862.1"
                     /translation="REKLEKQIGELSAIVAELNADRKKLKEEEENLRKEMNSMGKEVA
                     SLRAEKEKLVTQVDTYKRELSELKEQIISYRAQSEMLKKDIAKKEEELRFLRNQKIIF
                     QGREVLLVETVKGPKSEQEARDILQYLIEKTKDIVRYRYLTIDENPPKEFSFIYDPED
                     IEFAVESLKKDQQRLIRIRVKNNTLIGEPINLSIESLKSKLVFSKNEVITFKFISAKL
                     PRNKIIEELNLLLKDVEREGLRRGMLPDPITGPLGSVPYESLLNLSVRIKVQYSKTGS
                     ALVVAKASSDIYTAGPLSLKFELR"
     gene            complement(1028..1294)
                     /locus_tag="P5310_05575"
     CDS             complement(1028..1294)
                     /locus_tag="P5310_05575"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HRU32863.1"
                     /translation="MKRKNLEKIKGEIESLILYAKKRENIEAIYLFGSSGTEYETAFS
                     DIDIAILYGTYITLEEELSVECDICKIFSITSKSFLNFIFIRIK"
     gene            complement(1315..>2729)
                     /locus_tag="P5310_05580"
     CDS             complement(1315..>2729)
                     /locus_tag="P5310_05580"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HRU32864.1"
                     /translation="IIAHPELKYNVIGFVDDSETKLGGFIHDVKVIGKVEDIPDIAKQ
                     YKIDTIIIAIPSASGKDMSRIYSYTSKTRAKVFVVPGLYDLIGSKVSISTLRPFGLQD
                     LLTRDPVRLDLNMARGYLGGKKVLVTGACGSIGKIICQQLISLGVELIALDNNETGIF
                     DLEKDLSLYSKITPVIGDIRFKERLKSIFTTYKPEIVFHTAAYKHVPLMEIYPEEAIL
                     TNVVGTLNLIEVCQESRVKQMINISTDKAVDPVNVMGMTKRFTEIMSIKANGDGTQFS
                     TVRFGNVLGSRGNVIEVWKKELEQGQPLSITSPNMKRFFMLTEEAASLVIEASCMAAP
                     GELLVLDMGEQIPILELAKIFCETQGYQLNTDINVKYIGLRPGEKLEEKLWADNEKVD
                     KTTHSKIMKVISPVPDLSWNEIRSIVRRMEALIREGKIEKAKEELKKMTSNSKNKFLK
                     KEEDIQPFPKMMEEGKRFNP"
BASE COUNT          886 a          542 c          448 g          853 t
ORIGIN      
        1 agagagaagc tggagaaaca gataggagag ctctcagcga tagttgcaga attaaacgca
       61 gatagaaaaa aactgaaaga agaagaagaa aatctcagaa aagagatgaa ctctatggga
      121 aaagaggtgg catctttaag agcagagaaa gagaagttgg taacgcaggt agatacttac
      181 aagagagaat tatctgagtt aaaggagcag ataatttcat atcgggctca atcggagatg
      241 cttaagaaag atattgctaa aaaagaagag gaattaagat tcttgagaaa tcagaagatt
      301 atttttcagg ggagagaggt tcttcttgtt gaaacagtaa aaggtccaaa gagtgaacag
      361 gaagcaagag atattcttca atatcttatt gagaaaacaa aagatatagt gagatataga
      421 tatctcacta tagatgaaaa tccacctaaa gaattttctt ttatttatga tcccgaagac
      481 attgagtttg cagttgaatc cctgaaaaag gaccagcaga gattgatacg tataagagtt
      541 aaaaataata cacttatagg agaacccatt aatctcagta tagaatctct aaagagtaag
      601 ttagtcttca gcaaaaatga agttataacc ttcaaattta tttctgccaa actccccagg
      661 aataagatta tagaagagtt aaatcttctc ttaaaagatg tagaaagaga agggctaaga
      721 agaggcatgt taccagaccc tataactggt cctttgggaa gcgtcccata tgaaagcctt
      781 cttaatcttt cggtcaggat aaaggtacag tactctaaaa ctggaagcgc actggtagtt
      841 gccaaagcta gcagcgatat atatactgca ggtccattga gcctgaaatt tgagctgcga
      901 taaagattaa ttaattgagt cggataatgt atcttatgta aagtaaaaaa atttaaacat
      961 ttctaataga ctctaaagct ttaactttta aattacaaaa ccttttaatt tacaaaattc
     1021 acaaggatta ttttattctt ataaaaataa aatttaaaaa agattttgag gttatggaga
     1081 agattttaca gatatcacat tctacagaaa gctcctcttc taaagttata taagtaccat
     1141 acaatatagc gatatctata tcactgaatg ctgtctcata ctcagttcca gaagatccaa
     1201 atagataaat agcctcaata ttctcccgtt ttttggcata taaaattaaa ctttctatct
     1261 cgcccttaat tttctccaaa ttcttccttt tcatatattt ctaaagccat ctcttcaggg
     1321 attaaacctc ttcccttcct ccatcatctt ggggaaaggc tggatatcct cttctttctt
     1381 caagaattta tttttcgaat tactagtcat cttttttaac tcttccttag ccttctctat
     1441 cttcccttcc ctgataagag cctccattct tctcactatg gagcgtatct cattccagga
     1501 aaggtcaggc actggggata taaccttcat aatcttggaa tgagtagtct tatctacttt
     1561 ctcattatct gcccagagct tctcctccag cttctcccct ggtctcaatc cgatgtattt
     1621 tacatttata tcagtgttta actggtagcc ctgcgtttca cagaagattt tcgccagttc
     1681 cagaatagga atctgttctc ccatatcaag tacaagaagt tctcctggag ctgccataca
     1741 gctagcctca attaccagag atgctgcctc ttctgtaagc ataaagaatc tcttcatatt
     1801 aggacttgtt atacttaatg gttgtccctg ttccagttct ttcttccaaa cctctatgac
     1861 atttcccctg ctccccagca catttccaaa ccttacagtt gaaaattgag tcccgtcacc
     1921 atttgccttt atagacataa tctcagtaaa tctctttgtc atccccataa catttacagg
     1981 gtcaacagcc ttatcggtag atatatttat catctgtttt actcttgatt cctggcatac
     2041 ctctatcaga ttcaaggtcc ccacaacatt agttaatatt gcctcctcgg gataaatctc
     2101 catcaggggc acatgcttat aagcagctgt atgaaagact atctcaggtt tatatgtagt
     2161 aaatatgctt tttaatcttt ctttgaatct tatgtcccca attaccgggg taatcttact
     2221 gtataaactt aaatctttct ccaggtcaaa gatccctgtt tcattattat ccagagctat
     2281 caactccaca ccaagtgata taagttgctg acagatgatc ttacctatag aaccacaggc
     2341 tccagtaacc aaaacctttt taccacccag atatccccgt gccatattca aatctaacct
     2401 taccgggtct ctggtaagaa ggtcctgtaa tccaaagggt ctcaacgtgg atatggaaac
     2461 tttggaacct ataaggtcat acaatcctgg aacaacaaaa actttagccc tggtctttga
     2521 ggtataagaa tagattctgc tcatatcctt tccagaagca ctgggaatgg caattataat
     2581 agtatctatc ttatactgtt tggctatatc tggaatatct tccactttac ctatgacctt
     2641 tacatcatgt ataaatccac ctaatttagt ttcactatca tctacaaaac caataacatt
     2701 atattttagt tctggatgag ctattattt
//
LOCUS       DAOTKU010000211         2717 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_30482_length_2717_cov_2.845980, whole genome shotgun sequence.
ACCESSION   DAOTKU010000211 DAOTKU010000000
VERSION     DAOTKU010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30482_length_2717_cov_2.845980"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /locus_tag="P5310_05585"
     CDS             complement(<1..858)
                     /locus_tag="P5310_05585"
                     /inference="COORDINATES: protein motif:HMM:NF013314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRU32865.1"
                     /translation="MKIFDVIVVGAGHAGIEAALSANRLGCSVLLLTGNLDTMAWMPC
                     NPAIGGPGKGHLVREIDALGGEMGRATDATYMHIRVLNSGKGIAVRAYRAQVEKKFYH
                     IYMKEKLENTPNLILRQAQVTDILFDEDRVIGVRTRTNQEFYSQTVIVTTGTFLGGRI
                     FIGTDVSYPAGRAGEPPSNELTESLRKLGFRIGRFKTGTPPRIDGRTIDFSKMQEQPP
                     SEVPLRFSYRTELAEVAGRKQLSCWLTFTTERTREIVLENIDRAPMYAGQIKGTGTRY
                     CPSFETKVVH"
     gene            complement(855..2210)
                     /gene="mnmE"
                     /locus_tag="P5310_05590"
     CDS             complement(855..2210)
                     /gene="mnmE"
                     /locus_tag="P5310_05590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HRU32866.1"
                     /translation="MNDTIVALSTPYGESGIGVIRLSGPLSLDLAQRIFKSRKRLSDV
                     PSHRAVFGYIIDPKTGDRLDECLLLVLRFPHSYTGEDTVEISVHGSPILEELIIKILI
                     ELGARLALPGEFTKRAFLNGKLDLSRAEAIIDIIQSRSEVGLKSSYIQLQGKLSEELR
                     PIIDMLKDLIAELEAGIDFPEEVDFSYEKLEDKIEYIVERLCKILNGAYFTRLYRRGL
                     YCPIVGRTNVGKSSLLNVLLGEERAIVTPIPGTTRDTIIESISIEGIPVTLVDTAGIR
                     DTQDLVESIGVDRTLSVIENADIVIWVIDVTESLREDDLEIERLTREKKRILVLNKID
                     VNNRVIYTDDIRDLGPVIEISCKTGEGIDGLKLKLKEILGEVPSPNQVIPINSRHEAL
                     IKRCIDVLSDVLNGIRSRLPVDVFAGDIWESYNILGEITGETADIDIVEEIFSRFCVG
                     K"
     gene            complement(2203..2679)
                     /locus_tag="P5310_05595"
     CDS             complement(2203..2679)
                     /locus_tag="P5310_05595"
                     /inference="COORDINATES: protein motif:HMM:NF024483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HRU32867.1"
                     /translation="MLRQKPSVDSLAKMFINQLLAPYKFKYDISLELKDNIYWVKLQG
                     EDISFLIGKRGVILYSLQHLLRLYVRRSIDEEVLLILDINGYREDKERKLITQADRAA
                     LRVKRTGRKFVFKPMPAALRRVIHKALENSPDVITYSEGKEPNRRVVVALKQKRDE"
BASE COUNT          751 a          669 c          407 g          890 t
ORIGIN      
        1 atgcacaacc ttggtctcaa aagagggaca gtatctggtg ccagtaccct ttatctgtcc
       61 tgcatacatg ggtgccctat cgatgttctc taaaactatc tcccttgtcc tctcagtggt
      121 aaatgtaagc cagcaggata actgttttct cccagcaact tctgccagtt ctgtacgata
      181 agaaaatctc aatgggactt cacttggagg ctgttcctgc attttggaga aatcaatcgt
      241 ccttccgtct attcggggag gagttccagt tttaaatcta ccaattctaa agcctaactt
      301 tctgagagat tccgtcagtt cattacttgg aggctcccca gccctaccag ctggataaga
      361 gacatcggta cctataaaaa ttcttcctcc gagaaaagtc cctgtggtta caattactgt
      421 ttggctataa aattcctgat tagtcctggt cctcacacct atcactctat cctcatcaaa
      481 taagatatca gtaacctgag cctgtctcag aataagattt ggagtatttt ctaacttttc
      541 tttcatatat atatgataga attttttctc tacctgagcc ctgtaagccc tcactgctat
      601 acccttacct gagttgagga cccttatatg catataggta gcatctgtag ctctacccat
      661 ttcgccacct aaggcatcta tctctctgac aagatgtcct tttcctggtc ctccaatagc
      721 aggattacag ggcatccatg ccatagtatc tagattccct gttaaaagca aaacactgca
      781 tcccaatcta ttagcagaca gggctgcttc tattccagca tgccctgcac ctacaacgat
      841 aacatcaaat atcttcattt tcccacacaa aacctcgaaa atatctcttc tactatatct
      901 atatcagctg tctctcctgt aatctctccc aatatattat aactctccca tatatctcct
      961 gcaaaaacat ctacaggcag tctagatctg ataccattca agacatctga gagtacatct
     1021 atgcatctct ttattaaagc ctcatgcctg gagttaattg gaattacctg atttggagac
     1081 ggtacttctc ccagtatctc cttcaatttt agtttcaacc cgtctatccc ttctccagtt
     1141 ttacaggata tctctattac aggtcctaag tctctaatat catcagtata tataactcta
     1201 ttattaacat ctattttatt taaaactaat atccgctttt tctctctggt caatctctca
     1261 atctccagat catcctctct caatgattcg gttacatcta taacccatat gactatgtct
     1321 gcattctcta tcacagatag tgttctatct acaccaatgc tttccaccag gtcctgcgta
     1381 tctctaatac cggctgtgtc aactagagta acaggaatac cttctataga tatggattca
     1441 attatagtat cccttgtcgt tccaggtata ggggttacta tagctctctc ttcacccaaa
     1501 agtacattga gaagactaga ttttcccaca tttgttctgc caactatagg acagtaaagc
     1561 ccccttctgt aaagtctagt gaaatacgca ccatttaaaa tcttacaaag tctctcgact
     1621 atatattcta tcttatcttc cagtttttca taagagaaat caacttcttc aggaaaatct
     1681 atccctgctt ccagctctgc tattaaatct ttcagcatat ctataattgg tcggagttct
     1741 tcgctcaatt tgccctgtaa ctgaatatat gaagacttga gcccaacttc agatctggac
     1801 tgtataatat ctataattgc ttctgctctg gagaggtcga gttttccatt taagaaggct
     1861 cttttagtga attctcccgg taaggctaat ctggcaccaa gttctataag aattttaata
     1921 atcaactctt ccagtatagg gctcccatgg acagaaattt ctaccgtatc ttccccggta
     1981 taactgtgag gaaacctcaa aaccagcaag aggcattcat ccagtctgtc tccagttttt
     2041 ggatcaatta tatatccaaa aactgccctg tgagatggga catcacttag tctcttcctg
     2101 cttttaaata ttctctgagc aaggtccagg gagagaggac cactgagtct tatcacccct
     2161 atgccagatt ctccataagg ggtagagaga gctacaatgg tatcattcat ctctcttctg
     2221 ctttaaagct accacaaccc tcctgtttgg ctccttaccc tcactataag ttataacatc
     2281 aggactattc tccagagcct tatggattac tctccttaac gctgctggca taggcttaaa
     2341 gacaaacttt cttccggttc ttttaaccct taatgcagcc ctatcagcct gagtaataag
     2401 ttttctttct ttatcctctc tatatccgtt aatatctaaa attaataaaa cctcctcgtc
     2461 tatacttctc cttacatata gccttaatag gtgttgcagg gaatacagta ttacacccct
     2521 tttgccgata agaaaagata tatcttctcc ctgtaacttc acccagtata tattatcttt
     2581 caattctaac gatatatcat acttaaattt atacggagct aataactggt taataaacat
     2641 cttggcaaga ctatctacac taggcttctg tctcagcata tatacctcca ttgattaagg
     2701 attattttaa taccttc
//
LOCUS       DAOTKU010000212         2702 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_30673_length_2702_cov_2.862108, whole genome shotgun sequence.
ACCESSION   DAOTKU010000212 DAOTKU010000000
VERSION     DAOTKU010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30673_length_2702_cov_2.862108"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            190..1017
                     /locus_tag="P5310_05600"
     CDS             190..1017
                     /locus_tag="P5310_05600"
                     /inference="COORDINATES: protein motif:HMM:NF025224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HRU32868.1"
                     /translation="MKRYPGFLVFLLGISLFIVNAGYGKDSEIISFADPNLDRMIRNV
                     LGIPQEQAITKEKLLKLTELKAGSEGIKNLSGLEYAINLQSLYLEDNEIVDLAPLSEL
                     TRLQQLSLGLNKIKDLTPLAELASLRWLGLGSNQIEDISPLTNLKNLQELWLPINQIS
                     NITPLSSLTSLQRLDLSHNQIIDINPLAGLTNLEELNLRNNQISNISSLINLTRIRIL
                     DLSNNWINDITPLVSNPGLGQGDIVYLQNNNLDIASNHSNLLNIQLLQNRGVKVYYE"
     gene            1072..>2702
                     /locus_tag="P5310_05605"
     CDS             1072..>2702
                     /locus_tag="P5310_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32869.1"
                     /translation="MSIEHYQKAVRALDSYLPEIISKQVDDPNRFDYGGIEKPSLGIA
                     DSEYGINELLSCYFCPDSKYYRDIDLRERVIKAVKFLVRTQHDDGTIDLYETNFHSPP
                     DTSFRVWVYAPWVEYLRKIKLNNSELEILSLLETFLKRTIPALKSGGFHTPNHRWVQA
                     SALARLGVLFDDKECKLLAEEYLEEGIDCNEDGLFYEKSLAIYNPVCGMAMLWLAEDL
                     GKEELIKYTKKVLDFAIHFLEKDGTVLTTFSIRQDRGLRVNLDTRYYYLYKKMSILEN
                     NGTYAEASDCIFNGNLNRLGHGFNPLYLFLFYPEFKEERIERKPLPRFGEYFFKNSGI
                     VRINSDKYSLTFTKGSDEFLTLVLGAADIRFRYLTSFFGKGPFIGEDMEGVEDGYILS
                     QKLEWGYLDLLPEEERGKKVMWNEMNHSLRRWIKMQNIETRIKLCMNNNLQSGNISLS
                     TNGCRDVLTMLEVSVPKDGEISFKGEVTQVRENTFLLKDGLVRYQKGSISFELSPGSG
                     NHKLIDYRGDRATKEAGHLYIVMTFRVPFEHKLEFS"
BASE COUNT          934 a          424 c          553 g          791 t
ORIGIN      
        1 ttaaaaatat aaagctatgc gttggtggaa tcgtagttct taacaacaag gtattatttg
       61 tccgtcagac atatgggaac cttaaaaatg ttttactgaa caaaactgaa acacaataat
      121 atgctcttta atccagtctc cttatatagg agaccatatg tgaaaggatg atacttagga
      181 ggcattatta tgaaaagata tccaggtttt ttagtctttc tgctaggcat cagtctattt
      241 atagtgaatg cgggatatgg gaaagattca gagataatat catttgctga tcctaatctt
      301 gatcggatga taagaaatgt tttagggata cctcaagaac aagctattac aaaggagaag
      361 ctcttaaaat taactgaatt aaaagctggt tctgaaggta taaaaaattt atccgggctt
      421 gaatacgcaa ttaacttaca gtctttatat cttgaggata atgagatagt tgatcttgcc
      481 cccctatctg aacttaccag attacaacaa ttgagtctcg ggttaaataa gataaaagat
      541 ttaactcctc tagctgaact tgccagtttg cgatggctgg gtcttggatc gaatcagata
      601 gaagacatca gccctttaac aaatcttaaa aacttacaag aattatggct tccaattaat
      661 cagataagta atattacacc tttaagcagc cttaccagct tacaacgttt agaccttagc
      721 cataaccaga taatagatat taatccccta gccgggctta ctaatttaga agaattaaat
      781 cttcgtaaca atcaaataag taatatcagc tctctaatta atctgacaag aataagaata
      841 ctggacctga gcaataactg gataaatgat atcacgcctc tagtcagtaa tcctggattg
      901 ggacagggag acatagttta ccttcagaat aataatctag atatagcttc aaatcatagc
      961 aatctgttaa atattcaatt actccagaac agaggcgtaa aggtatacta tgagtaggat
     1021 cagtattata tccatattta acgcaaatat taaagatatg ggaggactta tatgtcaatc
     1081 gagcattatc aaaaagctgt aagagcatta gattcatatt taccagagat tataagtaaa
     1141 caggttgatg atccgaaccg tttcgattac ggggggatag aaaagccaag ccttggtata
     1201 gcagattcag aatatggaat aaatgaacta ctaagctgct acttttgtcc tgattctaag
     1261 tattatcgtg atattgatct gagggaaagg gtaatcaaag cggtcaaatt tctagtcaga
     1321 acccagcatg acgatggaac tatagactta tatgaaacga attttcactc tcctccagat
     1381 acatctttca gggtatgggt ctatgcccca tgggtcgagt atcttagaaa aataaaactg
     1441 aacaattctg agctggagat tctttccctt ttagaaactt tcctgaaaag aacaatacct
     1501 gcattaaaaa gtggaggatt tcatacccca aaccatagat gggtccaggc ttctgcactg
     1561 gctagacttg gagtactttt tgacgataaa gaatgtaagc ttttagctga agaatatctt
     1621 gaagaaggta tagattgtaa tgaggatggt cttttttatg agaagagcct tgccatatac
     1681 aatcctgtat gtggtatggc tatgctctgg ttagctgaag acttgggtaa agaagaactt
     1741 ataaagtata caaagaaggt tttggatttt gctattcatt ttctagaaaa ggatgggact
     1801 gttctcacca ctttttcgat tagacaggat agaggattga gagtaaattt ggacacaaga
     1861 tactattacc tctataaaaa gatgagtatc cttgaaaata acggtaccta tgcagaagcc
     1921 agtgactgta tctttaatgg taatttaaac agactgggtc atggatttaa tcccttgtac
     1981 ctatttttat tttatccaga atttaaagaa gagcgtatag agaggaagcc tctgccaaga
     2041 tttggagaat atttctttaa aaatagtgga atagtcagaa taaactcgga caaatatagc
     2101 ctgactttca caaaaggcag cgatgaattt ctaacattgg tattgggagc agctgatatc
     2161 agatttcgat atctcacgtc tttcttcggg aaaggtccat ttattgggga agatatggaa
     2221 ggggttgaag atggatatat attaagtcaa aaacttgaat ggggttatct agaccttctc
     2281 cctgaagaag agaggggaaa gaaggttatg tggaatgaga tgaaccactc attaagaaga
     2341 tggataaaga tgcaaaatat agagaccagg ataaaactct gtatgaataa taatcttcag
     2401 agtggtaata tcagcctgtc tacaaatgga tgtagggatg tacttacaat gttagaggtc
     2461 tcagtgccaa aagatggaga gatatcgttc aaaggagaag taactcaggt aagagaaaat
     2521 accttcttgt tgaaggatgg actggtaaga tatcaaaaag gcagcatctc ttttgaattg
     2581 agcccagggt caggaaatca taagcttatt gattacagag gagatagagc cacgaaagaa
     2641 gccggacatc tatatatagt tatgaccttt cgtgtaccat ttgagcataa attagagttc
     2701 tc
//
LOCUS       DAOTKU010000213         2693 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_30794_length_2693_cov_3.719864, whole genome shotgun sequence.
ACCESSION   DAOTKU010000213 DAOTKU010000000
VERSION     DAOTKU010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30794_length_2693_cov_3.719864"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1875)
                     /locus_tag="P5310_05610"
     CDS             complement(<1..1875)
                     /locus_tag="P5310_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HRU32870.1"
                     /translation="MEQKAGYLNPELPIEERVEDLLSRMTLEEKVGQLKAKMLHVWKK
                     LADIFEGLDEEQVRKFDKVFSELFRDRELVEAISSNLWKKQWREIIMEGDKEKYPIGE
                     LSCALRPFSPKDGAELANEIQKHILENSRIKIPVLIHDECLHGCMAKGSTSFPQSIAI
                     ASTWNPELMHEVATAIGKETRARGIHQALSPTINIARDPRCGRTEETYGEDPYLASKM
                     AVSFIRGLQEQGVITTPKHYAANFVGDGGRDSNAVHFSERLLREIYFPAFKAAIEKGD
                     ALSIMSAYNSLDGIPCSSNKWLLTDILRKEWGFKGYVVSDYGAVIHLMTKHRVASSKA
                     EAAQKALEAGMDMELPESDCFEEVLGLVKEGKLSEDVLNEAVRRILRVKFWLGLFDNP
                     FIDPEYAEKVCDCKEHRELALKVARESIVLLKNEGILPICKEIKSIAVIGPNANVPRL
                     GGYSGYGIKVVTPLEGIENKVGKNVEVYFAEGCKLNDPSRDGFNEAIDIAKKSDVAIL
                     FMGNSVPETEGEQKDRCNLDLPGAQEDLIKEICDTGTPVIVVLINGSAITMTKWIDGV
                     KAILEAWYPGEEGGNAIADILFGDYNPGGKLPITFPRYTGQLPLYYNYKPSGRVDDYV
                     D"
     gene            complement(1899..>2693)
                     /locus_tag="P5310_05615"
     CDS             complement(1899..>2693)
                     /locus_tag="P5310_05615"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HRU32871.1"
                     /translation="EAIRNTDRKHIIFIEGNRHSQVFEGLDEPFDDNLVYSSHNYTIA
                     THRASRYPGYVGDVYTDKNWMEKIFLERNRWILEHKRPSWVGEFGALFDGPVNSPTNA
                     DKARLLALKDQLEIFNKYEQHWTIWTYKDVGVQGLVVPKEDSEYMRRIRPVSELKKKL
                     GLDPWTSRGYGLLRGETARMVETIAQAVSEIVKDYSLDYTSLTRELGDRLLSGAISSF
                     LAPLYAFQFMDMTEEEIEKMHHMAFEFSNCEKRLYLIEVLESALKN"
BASE COUNT          677 a          672 c          444 g          900 t
ORIGIN      
        1 gtctacataa tcatcaaccc ttccggaagg cttgtagtta tagtataggg gtaattgtcc
       61 tgtatacctg gggaatgtaa tggggagttt ccctccagga ttatagtctc caaagagtat
      121 atctgctatg gcattacccc cttcctctcc tggataccag gcttccagta tagcctttac
      181 cccatcgatc catttagtca tagtgatagc gcttccattt atcaggacta ctattacagg
      241 tgtaccggtg tcgcatatct ctttgatcag gtcctcttga gctccaggta gatctagatt
      301 gcatctgtct ttctgttctc cctctgtctc agggacagaa ttacccataa atagtattgc
      361 aacatcgctc ttcttagcta tatctatagc ttcattaaag ccgtcccttg atgggtcatt
      421 caatttacat ccttcagcaa aatatacctc tacgtttttc ccaaccttat tctctatccc
      481 ttccagagga gttactacct ttataccgta cccactgtaa ccgcctaacc ttggaacgtt
      541 tgcatttgga cctatcacgg cgatggattt tatctcctta catatgggca gtatcccctc
      601 attcttaagt aagacaattg attctctcgc cactttcaat gctaactctc tgtgttcttt
      661 gcaatcgcaa accttttctg catattctgg gtcaataaat ggattgtcaa agagacctaa
      721 ccagaatttt accctaagga ttcttctgac agcttcgttt aatacatctt ctgagagttt
      781 tccttcctta accaagccca ggacttcttc aaaacagtca ctttctggaa gttccatatc
      841 cataccagct tccagggctt tttgggcagc ttcagcctta gaacttgcaa ctctatgttt
      901 tgtcatcaga tgaatcaccg ctccatagtc agacacaacg taccctttga atccccactc
      961 ctttctcagg atatctgtta gtagccattt gttagaagag cagggaattc cgtccaggga
     1021 attgtatgca gacattatag aaagagcatc tcctttttct atagcagctt taaaagcggg
     1081 gaagtatatc tctctcaata gcctttctga gaaatgaacc gcattactat ctcttccccc
     1141 atctccaacg aagtttgccg catagtgttt aggtgtggta ataactcctt gttcctggag
     1201 acccctgata aatgatactg ccattttact agccagataa gggtcttcgc catatgtctc
     1261 ttctgtcctt ccacatcgcg ggtctcttgc tatgtttatc gtaggagaga gagcctgatg
     1321 aatacctctg gctcttgtct cttttcctat agcggtagcc acctcatgca ttaattctgg
     1381 attccaggta cttgctatag ctatagactg tgggaaactg gtagagcctt tagccataca
     1441 gccgtggaga cattcatcat gaatcaatac gggtatcttt attctactat tctccagtat
     1501 atgtttttgt atctcattag ccagctcagc tccatcttta ggagagaatg gtctgagagc
     1561 acagcttaat tcaccgatag gatatttttc tttatccccc tccatgatta tttctctcca
     1621 ttgttttttc cagagattac tggagatggc ttcaaccagt tctctgtctc tgaataactc
     1681 ggagaatacc ttgtcgaact ttcttacctg ctcttcatct aacccctcaa agatgtctgc
     1741 cagtttcttc cagacatgta gcatctttgc cttgagctga cccaccttct cttccagtgt
     1801 catcctggag aggagatcct caactctctc ttcaattgga agctctggat ttagataacc
     1861 tgccttttgt tccataataa cctcctgctg atatattttt aattttttaa agcactctct
     1921 aacacttcta tcaggtagag tctcttctca cagttagaaa actcaaatgc catatgatgc
     1981 atcttttcta tctcttcttc agtcatatcc ataaactgaa atgcgtatag tggtgcaagg
     2041 aagctactaa tagcaccact cagtaatcta tcccccaatt ccctggtaag tgaggtataa
     2101 tcaagactat agtcttttac aatttcagat accgcctgag ctatggtctc taccatcctt
     2161 gcagtttcgc ccctcagtaa tccataacct cttgatgtcc aggggtcaag ccctaacttc
     2221 ttcttcagtt cagagacagg tctgatcctt ctcatatatt cactgtcttc cttaggaact
     2281 actaatcctt gaacccccac atccttataa gtccagattg tccagtgttg ttcatactta
     2341 ttaaatatct ccagctggtc ttttaatgct aaaagtctgg ctttatccgc atttgtaggt
     2401 gaatttacag gtccatcaaa cagagcgcca aactcaccta cccaagaagg tcttttatgt
     2461 tctaaaatcc atctattcct ctcaagaaat atcttttcca tccaattctt atctgtatat
     2521 acatccccaa cataacctgg atatctactt gctctatgag ttgctattgt ataattgtga
     2581 gaactgtata ctaaattatc gtcaaacggt tcatctaatc cctcgaagac ctgagaatgt
     2641 cgattaccct caataaatat tatatgtttc ctatccgtat ttcttattgc ttc
//
LOCUS       DAOTKU010000214         2684 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_30927_length_2684_cov_2.287181, whole genome shotgun sequence.
ACCESSION   DAOTKU010000214 DAOTKU010000000
VERSION     DAOTKU010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2684
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30927_length_2684_cov_2.287181"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..166
                     /locus_tag="P5310_05620"
     CDS             <1..166
                     /locus_tag="P5310_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-amino-4-imidazole-
                     carboxylate carboxylase"
                     /protein_id="HRU32872.1"
                     /translation="AVPTSTGYGASFKGLSALLTMLNSCVPGIAVVNIDNGFGAGYLA
                     SIINHMGEKE"
     gene            163..1335
                     /gene="larC"
                     /locus_tag="P5310_05625"
     CDS             163..1335
                     /gene="larC"
                     /locus_tag="P5310_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HRU32873.1"
                     /translation="MKIIYFDCFNGISGDMILGALFDLGLDKSLWDRELSKLGISGYT
                     VRVEKKRKGPIMGTDAEIVIEDKVTHRHAKEIIDTVRDSSLDSDIKERSIRILTRIAE
                     AESAIHNEPLEEVHLHELGGIDTIVDVVGTVTGLKILGVERVYSSPLPLGEGFIDTAH
                     GRLPVPAPATAELLKGVPVYSNGMEGELVTPTGAGIISTLSSSFGPIPAVKIERIGYG
                     SGKRDFPIPNMLRAIMGEVNEKKQRDTNTVIDVNIDDMNPQICGYVMDKLFNAGALDV
                     FFTPIYMKKNRPAIKLTIIAPDYAINTLVDIIFKETTSIGVRAYQVEKIMLPREIIDI
                     DTPWGDVRVKVSYINGTPKIMPEYDDCRVIAEKTGLSIQDIMREVKGLAAERIKLA"
     gene            complement(1338..2171)
                     /locus_tag="P5310_05630"
     CDS             complement(1338..2171)
                     /locus_tag="P5310_05630"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HRU32874.1"
                     /translation="MRVTFIGHLSKDINIMKDKEQIIPGGGVYYGAFVTRSFGAETYV
                     YTKLSSKDTELFKDMESYGIRLVKFTSKVTTTIQNLYPTDNPDDRISKMVERADPFTQ
                     EDIKIIEKSDVLHISGLWHGEIPENLIESLREKTDLLGLDAQGFLRNVDESGDMIYKD
                     WQDKERYLPLIDVFKVDSRESEILTGEKDMEKALGKIARFGPKEIILTYKDGIKLWIQ
                     GEILEEKFGGWTLEGRTGRGDTCMAGYLVHRHTGYRNALRKAAEIATEKMKRPGPYRK
                     D"
     gene            2278..>2684
                     /locus_tag="P5310_05635"
     CDS             2278..>2684
                     /locus_tag="P5310_05635"
                     /inference="COORDINATES: protein motif:HMM:NF016114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HRU32875.1"
                     /translation="MKKAIIINTIMKDWEYIHTVAKAALLYYMEEKTQEEIAEQLGIS
                     RPTVSRMLSYARKSGIVSIRLDIPNEVFPELEDKLEKIFSLEEVTIVSSSDNLSLLKQ
                     NLGRGGADLLKRRIKGIDSLGISWGSTLFEVVN"
BASE COUNT          800 a          525 c          551 g          808 t
ORIGIN      
        1 agctgttccc acaagtacag gttatggggc aagctttaaa ggtttatcag cactgttgac
       61 catgctcaac agttgtgtcc cgggaatagc ggtggtgaat atagataatg gctttggagc
      121 aggttatctt gccagtataa taaatcatat gggagaaaaa gaatgaaaat aatatatttt
      181 gactgtttta acggtataag tggtgatatg attctgggag ccctctttga ccttgggctt
      241 gacaaatctc tttgggatag agagttatct aaacttggca tatctggata tactgtaagg
      301 gttgagaaga aaagaaaagg tcccattatg ggtaccgatg ctgaaatagt cattgaagat
      361 aaggtcacac atagacatgc aaaggagata atcgatacag ttagagatag cagtttagac
      421 agcgatatta aagagaggag tattaggatt cttacacgta tagcagaggc agaatcggcg
      481 attcacaatg aacctctgga agaggtccac ctccatgagc ttggaggaat agataccata
      541 gttgatgtgg ttggtactgt taccggcctg aaaattttag gggtagaaag agtctacagc
      601 tcccctcttc ctttagggga aggttttatt gatacagccc atggaagact ccctgtccct
      661 gcaccagcca cagcagaatt gctaaaaggc gtccctgtat actccaatgg tatggaggga
      721 gaacttgtca caccaacagg ggctggtata atatctaccc tttcatcatc ctttggacca
      781 atcccagcag tcaaaataga aaggataggt tacggttcag ggaaaaggga ttttccgata
      841 ccaaatatgc tgagagccat tatgggagag gtaaatgaga aaaagcagag ggatacaaac
      901 acagtaatag atgtaaatat agatgatatg aaccctcaga tctgtggcta tgttatggat
      961 aagttattca atgccggtgc tctcgatgtc ttcttcacac ccatatatat gaaaaagaat
     1021 agacctgcga taaagcttac cattattgca ccagattatg ctataaatac cctggtggat
     1081 ataatattca aagagacaac atcgattggt gtaagagcct atcaggtgga gaagattatg
     1141 cttcccaggg agattataga catagatacc ccctggggcg atgtcagagt aaaggtttct
     1201 tatataaatg gaacccccaa gataatgcct gagtatgacg attgcagggt tatcgctgaa
     1261 aagacaggat tatctatcca ggatataatg agagaggtaa aaggactggc agctgagaga
     1321 attaagctgg cataaattta atcttttcta tatggaccag gtcttttcat cttctccgtg
     1381 gcaatttcag ctgccttcct cagagcgttt ctgtatcctg tatgtctgtg gacaaggtat
     1441 cctgccatac aggtatctcc tcttcctgtt ctcccctcta aagtccatcc tccaaatttc
     1501 tcttcaagga tttccccctg aatccagagt tttatcccat ccttataggt aaggataatt
     1561 tcttttggtc caaaccgggc tattttcccc agagcctttt ccatatcttt ttcgccagtt
     1621 aagatttctg attccctcga atccacctta aagacgtcta tcaaaggtag atatctttct
     1681 ttatcttgcc agtctttata aatcatatct ccactctcat cgacattcct caagaatccc
     1741 tgtgcatcca atcccaagag gtctgtcttt tccctcagag attctataag attctccgga
     1801 atttctccat gccataatcc tgatatgtga aggacatcgc tcttctctat aatttttata
     1861 tcttcctgcg taaatggatc agccctctca accatttttg atatcctgtc atccggatta
     1921 tcggtgggat agaggttttg aatggtggtg gtgacttttg aggtaaattt tacaagcctt
     1981 atcccatagc tttccatatc tttaaaaagt tctgtatctt ttgaagaaag tttcgtatac
     2041 acataagtct ctgccccgaa tgatctggtt acaaatgccc cgtaataaac tcctccacca
     2101 gggattatct gttctttatc tttcattata tttatatctt ttgataaatg acctataaag
     2161 gttactctca tattgtcgat ctccttcctt aatattttct ccagttatta tacctctgca
     2221 tctatactaa ataaacaacc tgtagtttcg ttgaacaaag tttcaaatat tgaaattttg
     2281 aaaaaagcta ttataataaa tactattatg aaagattggg aatatataca tacggtagca
     2341 aaggcagctc ttctctacta tatggaggag aagacccagg aggagatagc tgagcaactt
     2401 ggtatttccc gtccaactgt ctcaagaatg ctatcatacg caagaaaatc cggaatagtt
     2461 tcaataaggc tggacatccc aaatgaggta ttccctgagc ttgaagataa actggaaaag
     2521 atattctctc tggaagaggt tacgattgtc tcttcaagtg acaacctttc tcttttaaaa
     2581 cagaatcttg ggagaggtgg cgctgattta ctaaaaagga ggataaaggg gatagattct
     2641 cttggtatct catggggctc taccctgttt gaagttgtca ataa
//
LOCUS       DAOTKU010000215         2681 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_30967_length_2681_cov_2.928408, whole genome shotgun sequence.
ACCESSION   DAOTKU010000215 DAOTKU010000000
VERSION     DAOTKU010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30967_length_2681_cov_2.928408"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1344)
                     /locus_tag="P5310_05640"
     CDS             complement(<1..1344)
                     /locus_tag="P5310_05640"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRU32876.1"
                     /translation="MDKELDIIIIGGGVVGTAIARELSRYKLDVLLIEKESDVGWGTT
                     KSNSAVVHTGYDPKPGTLMAKLNAKGARLFPSICSELDVPYKKPGSMVLTPDEDGFKE
                     LEELKNRGKINGVTNLEIISQDELFALEPNISRDIKGALFVKDTAITSPVELAIALRE
                     NAEQNGVRFLFDTEVTGFEMENRRIISVKTSRGNLRSRWIINAAGIFSDEIARLAGDD
                     SFTITPVKGEYALFDKEVGNMVNHIIFPLPTETTKGILVLPTVDGNLLVGPNEHITTK
                     DDTTVTEEGLNEVLSFGKKEFPNLPLNMIITIFAGLRAKDLNRKDFIIESSPAVENLI
                     NVGGIQSPGLSSAPSISIYVTEILRDRGLDLIPDPGFTPERNGITRFSNLSWDERAEL
                     IAKDQNYGEIICRCEYVTVGEIIDAIRRGATTLDGIKFRTRAGSGRCQGGFCGPRL"
     gene            complement(1329..>2681)
                     /gene="glpK"
                     /locus_tag="P5310_05645"
     CDS             complement(1329..>2681)
                     /gene="glpK"
                     /locus_tag="P5310_05645"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: protein motif:HMM:NF000756.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HRU32877.1"
                     /translation="QNPDDIWSTIQEAIHLAIKKSEIDSREISAIGITNQRETTILWD
                     KITGKPLYNAIVWQCRRSADICEELRDRGLEPLIREKTGLLCDAYFSSTKIMWLLRNI
                     PELKDLIRENRVAFGTVDSWLLWNLTGGKVHATDTTNASRTMLFNITKCDWDDDLLDA
                     FDIPRSILPDVVDSAQVSGYTEDFPGRGIPVSGIVGDQQGALVGQCCFSPGSLKVTYG
                     TGCFLLEPMERFLISEKGMLTTIAWSINRSLVYAIEGSVFTGGSVVQWLRDNLGIIPD
                     SASSEVFARAVDDTGDVYFVPALTGLGAPYWDMYARGMIIGISRSTGREHIVRAALEG
                     IAYQIRDLVNTIEDVTEVKVQELRVDGGATGNSFLLQFQSDLLGIPVIRPRMIETTSL
                     GAFILASIGIGVFKDLEEAGKIWKVDRVFLPERNKDEMDRLYDRWKEAVRRSLGWTKN
                     "
BASE COUNT          750 a          691 c          431 g          809 t
ORIGIN      
        1 aagtctcggt ccacagaatc caccctgaca tctaccagaa ccagccctcg ttctaaactt
       61 tataccatcc aaagtagttg ccccacgtct tatcgcatca ataatttctc caacagtaac
      121 atattcacat ctgcatatta tttcaccata attctggtct ttagctataa gttcagccct
      181 ttcatcccag gaaagattag aaaatctagt tataccgttt ctctccggag taaatcctgg
      241 atcagggatc agatccaaac ctctatctct cagtatctca gttacatata ttgatatact
      301 tggtgcagag cttaatcccg gtgactgaat acctcccaca tttatcagat tctctacagc
      361 aggggaagac tcgataataa aatcttttct atttaaatct tttgccctca atccggcaaa
      421 gatggtaata atcatattga gcggtagatt gggaaactct tttttaccaa aagagaggac
      481 ctcattcaga ccctcttcag ttacggtggt gtcatcttta gtagttatat gctcattggg
      541 tccaaccagg agatttccat ccaccgttgg taagacgagg ataccctttg tggtctctgt
      601 tggtagcggg aatattatgt gatttaccat attcccaacc tctttatcaa agagggcata
      661 ctcacctttt acaggagtta tggtaaaaga atcatcacca gccaatctgg ctatctcatc
      721 ggaaaaaatc ccggcagcat ttattatcca tctggacctt aagttacctc tgctggtctt
      781 taccgatatt attcttcgat tctccatctc aaagcctgtc acttcagtat caaacaagaa
      841 ccttacccca ttctgttcag cattttctct caaggctata gctagctcta ccggagaggt
      901 aatggcagta tcctttacaa aaagagcccc ctttatatcc ctggagatat ttggttcaag
      961 ggcaaaaagt tcatcctgac taataatttc aagatttgta acaccattta tcttccccct
     1021 atttttcaat tcctccagtt ccttaaatcc atcctcatca ggagttaaca ccatagaacc
     1081 gggtttttta taagggacat ccaactccga acatatagaa ggaaataatc tggctccttt
     1141 tgcgttgagt ttagccatta aagtgccggg cttaggatca tatcctgtat gaaccacagc
     1201 gctgttcgat tttgttgtcc cccatcccac atccgattct ttttctatca gaagtacatc
     1261 caatttatat ctggaaagct ccctggctat tgcagttcct actactccac caccaattat
     1321 aatgatatct aattctttgt ccatcccaga ctgcgcctta cagcctcctt ccatctatca
     1381 taaagtctat ccatctcatc tttgtttcgc tctggaagaa acaccctgtc aaccttccat
     1441 attttacctg cctcttccag gtctttgaag acacctatac ctatagaggc aagaataaaa
     1501 gcaccaagag aagttgtctc tatcatcctg ggtcttataa caggaattcc aagaagatcg
     1561 ctttgaaatt gaaggaggaa gctattacct gttgcccctc catccactct cagctcctga
     1621 accttaacct ctgttacatc ctcaatggtg tttaccagat ctcttatctg ataggcaatc
     1681 ccctcaagag cagctctcac gatatgttct ctaccagtcg acctgcttat tccaattatc
     1741 atccctcttg catacatatc ccagtatgga gccccaagtc cggtaagagc tggaacaaaa
     1801 tatacatctc ctgtatcatc caccgccctt gcaaagactt cagaactagc cgaatccggg
     1861 ataataccaa gattatctct gagccattga actacggagc ccccagtaaa tacactcccc
     1921 tctatagcat agaccaaact tcgatttata ctccaggcta tagtggtcag catccctttt
     1981 tctgagatca aaaatctctc cattggttca aggagaaaac atccggtacc atatgtaacc
     2041 tttaatgagc caggagaaaa acagcactga ccaactaaag ctccctgctg atctccaact
     2101 atccctgata cagggattcc tctcccggga aaatcttctg tataacctga tacctgtgca
     2161 gaatcaacca catctggaag tatggaacgt ggtatatcaa acgcgtccag caggtcatca
     2221 tcccaatcac attttgttat attgaataac attgtcctgg aggcattggt ggtatcggtc
     2281 gcatggacct taccaccggt aagattccaa agtaaccagc tatcaaccgt tccaaatgct
     2341 accctgtttt ctctgattaa atcctttaac tctgggatat ttctcagtaa ccacataatc
     2401 ttcgtactgg aaaaatatgc atcacagaga agtccagttt tctctcttat aagaggctca
     2461 agccctctat cccttaattc ctcacatata tcagcacttc ttctgcactg ccagactata
     2521 gcattgtaga gaggttttcc agtaatctta tcccacagta tagtcgtttc cctctgattt
     2581 gttataccta tagcagaaat ttcacgagaa tctatctctg atttttttat cgcaagatga
     2641 atcgcttcct gaatcgtact ccaaatatca tctgggttct g
//
LOCUS       DAOTKU010000216         2675 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_31072_length_2675_cov_3.312977, whole genome shotgun sequence.
ACCESSION   DAOTKU010000216 DAOTKU010000000
VERSION     DAOTKU010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31072_length_2675_cov_3.312977"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="P5310_05650"
     CDS             complement(<1..341)
                     /locus_tag="P5310_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020034825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRU32878.1"
                     /translation="MEFREEFKTIFDRLRAEISKVIVGYEDIIEQVIIALFSGGHVLL
                     EGLPGLGKTLMVKTLADVLDLTFSRIQFTPDLMPSDIIGTNIMERDRAFRFQPGPVFA
                     QIVLADEINRAT"
     gene            complement(344..1690)
                     /locus_tag="P5310_05655"
     CDS             complement(344..1690)
                     /locus_tag="P5310_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32879.1"
                     /translation="MRELNKFISRVRLKIRSVLFFNWLLELYIYLSAIYIVNYFFHLD
                     VSYYYLLIPVFFAIFLSLVRPIDNRDVAIIIDKDLGLDERVITSLEFNGLDSPIVKRL
                     ISETVDMLRNLNLKNVYPFRFRKRLKYLLLIPPLLFLSLSLYQNVFYPGEVSPQVEES
                     GVSRISMDLITLQQKLVPERPDLAKRLEVLRKEMEEKRITSKEGINILEEITKEIDNT
                     PAGNMDSNARADIKKTLELVMEGMNDKGMSPATDNENSRKEEPQDSSGEGTGGKGMEA
                     GGDKPVDEEGGKTSPSYTGEEEDTSGAMSNEQGEQTGINEEEKSGADSAGGQIDGSAG
                     DSGEEVEKEGSLAGSDTVEEGGTLPGKGERDNKLGEETGRNQVQNILEYVPGIAKEEG
                     GIKLKIKGSGKETSPAVEGGTQGAPVKSVEDPVEGESIPYEYKEMIRIYFERLKGE"
     gene            complement(1674..2369)
                     /locus_tag="P5310_05660"
     CDS             complement(1674..2369)
                     /locus_tag="P5310_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32880.1"
                     /translation="MITIVLKDIEAKIDSPGMVKFWGIALFILFLVFVIASPGPQRVG
                     NSFIPKLFLVTGISEFFIFLLFTIYSTTGNSLVEEEKTIRDLILGGNFSVGSVVWGKL
                     ISFLLHFLILLVSTLPFNLASVNLGINGDMKSFYLIMITVDISLSCWGFFISTFKEKL
                     FGIILLWAGIAVLLILSARLPHPWNLINPLILIVNSKTDGLFYYLFIGISGLILTCWR
                     ATQLRRSKDERAE"
     gene            complement(2375..>2675)
                     /locus_tag="P5310_05665"
     CDS             complement(2375..>2675)
                     /locus_tag="P5310_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32881.1"
                     /translation="NLLNRSPYILEDAHLLYRGGYIHLGDIGIGDKTIKIGNERLSTI
                     KEEGVEGTILSWLKNNGIILSGDKNYILGWIHIPLPDFSLESSNSKFFTLCIVEI"
BASE COUNT          751 a          641 c          431 g          852 t
ORIGIN      
        1 gtagccctgt tgatctcatc tgccagcact atctgggcaa agacaggacc tggctgaaaa
       61 cggaatgccc tgtcacgctc cataatgttt gtccctatta tatctgaagg cataaggtca
      121 ggggtgaact gaatcctcga gaatgtaagg tccagtacat ccgccagtgt ctttaccatc
      181 agtgtcttac caagaccagg taatccctcc agtaatacat gtccaccact gaaaagtgct
      241 atgataacct gttctataat atcttcatat ccaactatca ctttagatat ctctgctctt
      301 aatctatcaa atatggtctt aaactcttct ctaaattcca tacctattct cctttcaatc
      361 tttcaaagta aatccttatc atctctttat actcatatgg aattgactcc ccttcgaccg
      421 ggtcttccac cgattttacg ggggcaccct gggttcctcc ttctacagca ggagaggtct
      481 cctttcctga gccctttatc ttcagcttga taccaccctc ttctttggct ataccaggta
      541 catactccag tatattctgg acctgattcc tgccggtttc ttctccaagc ttattatccc
      601 gttcaccttt cccgggtaat gttcctcctt cttcaacggt atcactgccg gctaaagacc
      661 cttctttttc tacttcttct cctgaatctc cagctgaccc gtcaatttga ccacctgcgg
      721 aatcagcacc tgacttctct tcttcattta tacctgtctg ttctccttgc tcattactca
      781 tagctccaga agtatcttct tcttccccgg tataactggg actggtcttt ccaccttcct
      841 catcgacggg tttatctcca ccagcttcca ttcctttccc acctgttcct tctccggaac
      901 tatcctgagg ctcttctttc ctggagtttt cattatcggt agcaggagac atccctttat
      961 cattcatacc ctccataacc agttctaatg tcttctttat atctgccctt gcattactgt
     1021 ccatattccc agccggggta ttatctatct cttttgttat ctcctccagg atatttatcc
     1081 cttctttcga tgtaattctc ttttcttcca tctctttcct caagacctct aatcttttgg
     1141 caaggtcagg tctctcggga accagtttct gctgtaatgt gataaggtcc atactgattc
     1201 tgctgactcc agattcttcc acctgaggtg atacctctcc gggatagaat acattctgat
     1261 agagagatag agagagaaat aatagagggg ggataagtaa tagatacttt agcctctttc
     1321 tgaacctgaa aggatagaca tttttaagat ttaaattcct cagcatatcc accgtttcag
     1381 atataagcct ctttactatt ggagaatcca gaccgttaaa ctccaaactg gtaatgaccc
     1441 tctcatcaag ccccaggtcc ttatcaataa taatagccac atccctgttg tctatcggtc
     1501 ttacaagaga gagaaaaatg gcaaaaaata ccggtattaa tagataatag tatgagacat
     1561 cgagatggaa aaagtaattt actatatata tggctgataa atatatatat agctccagga
     1621 gccagttaaa aaagagcact gatctgattt ttaatcttac tcttgatata aatttattca
     1681 gctctctcat cttttgatct cctcaattgg gttgccctcc agcatgtgag gattaaaccc
     1741 gatataccaa taaagagata gtaaaaaaga ccatcagttt tagaattgac tataagtatc
     1801 agcggattta tgagattcca aggatgaggc aaccttgcag atagaatgag taacaccgct
     1861 atacctgccc agaggagtat aattccaaac aatttctcct taaatgttga gataaaaaat
     1921 ccccagcagg acaggcttat atctaccgtt atcattataa gataaaaact cttcatatct
     1981 ccgtttattc caagatttac cgatgcaaga ttgaagggga gtgttgatac cagcaatatc
     2041 aagaagtgta ataagaatga gatcagtttc ccccagacca cactacctac agagaagtta
     2101 cctcccagaa tcaaatctct tatggtcttt tcctcttcta ccagagagtt gcctgtagtt
     2161 gaatagatgg taaataacag gaatataaaa aattctgaga tacctgtaac aaggaatagt
     2221 tttggtataa aactattccc cactctctgt ggacccggag atgctattac aaatacgaga
     2281 aagagtataa atagagctat accccagaac ttaaccatac ctggactatc tatctttgcc
     2341 tctatgtctt tcaatactat agtaatcata tctttcaaat ctccacgata cagagtgtaa
     2401 agaatttaga attagaagac tctagactga aatccggtaa cggtatatgt atccatccta
     2461 atatataatt cttatcacct gataatatta taccattatt tttaagccag gataaaattg
     2521 ttccctccac cccttcttcc tttatggttg acaatctttc attgccgatt ttaatagttt
     2581 tatccccaat cccgatatct ccaagatgga tatatccacc cctgtataag aggtgggcat
     2641 cctctaatat atatggagac ctgttgagta gatta
//
LOCUS       DAOTKU010000217         2650 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_31429_length_2650_cov_3.769171, whole genome shotgun sequence.
ACCESSION   DAOTKU010000217 DAOTKU010000000
VERSION     DAOTKU010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31429_length_2650_cov_3.769171"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1167)
                     /locus_tag="P5310_05670"
     CDS             complement(<1..1167)
                     /locus_tag="P5310_05670"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HRU32882.1"
                     /translation="MELGNVNEKVSEILSAIADFLEMKGENVYRVMAFRKAARTIAGL
                     PRRIESKEDVIVLPGIGKAIGDIVDEILRRGTSSLYEELIEEIPEGVREFLQIQGVGP
                     KTAFSIYKSTGASSLEELYELISTGRLKGTGNLKEKTIEKIYSGIEKAMEKKKLLRLD
                     QGYTIYTAVKDMLKNIPYVEDITLVGGLRRGKEINSDADILIASKEEERLKAIIDNMD
                     SKIDIKIVPPEMFPYYTFLYTGNNIHTKRIEEMIRDKRIFNSEEEIYHTIDLQYVPPE
                     LREGIGEIELASEYNLPALISPSDIKGDLHIHSLWSDGTANIIDMASACIKRGYRYMA
                     ITDHSASLKVAGGLSSEDIKEQHRELELIKDKLDGFTILKGIEVDILPDGTLDMP"
     gene            complement(1154..1639)
                     /locus_tag="P5310_05675"
     CDS             complement(1154..1639)
                     /locus_tag="P5310_05675"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HRU32883.1"
                     /translation="MSGSKISIDIVSVIFILLGLLWGIRKGFLRSSFFLIGILAGIYI
                     GNLCTRFFTADISNRFNISRDIAGAIVFIIVFFLALSLINGLGLLIKRPKRGLGKFID
                     RLGGGVIGIFWGFGICGFLGIILSRYEATKNLMPSTIIIGIAEFWVRKIVEGLLSYGT
                     W"
     gene            complement(1629..>2650)
                     /locus_tag="P5310_05680"
     CDS             complement(1629..>2650)
                     /locus_tag="P5310_05680"
                     /inference="COORDINATES: protein motif:HMM:NF036799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32884.1"
                     /translation="RRDISLEEDLYEEVVRIIGYDKVPSILHERITGVVSTSPAWEKR
                     KILRRKITSLGCSEVITSSFVPVQFDKYFREFYADDISSISVINPLREEESVLRWLLS
                     QSLLSLAINNVRWGYNDFNLFEIGKIFFKDGESFMEKEHLGIVQSGRIIYSWDKEVNA
                     SFFSTKGLLEKLFKHELTFVPAYYSVLHPGRTANILYREKVTGFIGELHPDVARILKL
                     PRIYIAEMDVDEFPLEFFSIGQVKEPSRLPKLKRDIAIVVPETLPSVEVTSILEEVFK
                     DTLEDYELFDLYKGPNIPEGTVNLAFSLNLRAKDRTLTQEEVEEKIELLKNKLADIGG
                     RLREW"
BASE COUNT          762 a          583 c          404 g          901 t
ORIGIN      
        1 aggcatatcc agcgtaccgt cagggagtat atcaacctct atacccttaa ggatggtgaa
       61 tccgtccaat ttatctttta tgagttcaag ttctctatgc tgttctttta tatcctcgga
      121 acttagtcct ccggcgactt ttaaagatgc ggagtgatct gttatagcca tatacctgta
      181 tccacgtttt atacaggcgc ttgccatatc tataatattg gcagttccgt cactccagag
      241 ggagtgaata tggaggtccc cttttatatc tgaaggagat ataagagctg gtagattata
      301 ttctgaagca agttctatct ctcctattcc ttctcttaat tcgggaggta catattggag
      361 gtctatagta tgatagatct cttcttcaga attaaaaatt cttttatctc taatcatctc
      421 ctctattctc tttgtatgga tattattacc cgtatataag aatgtataat acggaaacat
      481 ctcaggagga acaatcttaa tgtctatctt gctgtccata ttatctatta tagcttttaa
      541 cctctcttct tctttagagg ctataagtat gtcagcgtca ctatttatct ctttgcctct
      601 tctcagtcct ccaaccagag taatgtcctc aacataaggg atatttttaa gcatatcttt
      661 tactgctgta taaattgtgt aaccctgatc gagtctcaat aacttttttt tctccatcgc
      721 tttttctatt ccagaatata tcttttcgat agttttctct ttgaggttgc ctgttccctt
      781 taatctcccc gttgagatta attcataaag ttcttctaaa gatgaagccc ccgtagattt
      841 atatatgcta aatgcagttt tcggtcctac tccctgtatc tgaagaaact ctcttactcc
      901 ttctgggatc tcttcaatta attcttcata aagggaggat gtacctctcc tcaatatctc
      961 atccacaata tccccaattg ctttaccaat tccaggcaga acaatgacat cttctttact
     1021 ctctattctt cttggcaagc cagctatagt tctggcagct tttctgaacg ccataacgcg
     1081 atatacgttt tctcctttca tctccaggaa atctgctata gcagatagta tctctgatac
     1141 tttctcattt acgttaccaa gttccataag aaagaagccc ctccactatc tttctaaccc
     1201 agaactcggc aatcccgatt attatagtgc tgggcataag gtttttagta gcctcatatc
     1261 tggaaagaat gatccccaaa aagccacata ttccaaatcc ccagaaaata ccgataactc
     1321 cacctccaag tctgtctata aatttaccaa gtcctctctt aggtctttta ataagaagac
     1381 caagaccatt tatcagactt agggcgagaa agaatacaat tataaataca atagctccag
     1441 caatatctct agagatatta aatctattac tgatatcagc tgtaaaaaat ctggtacaga
     1501 gatttccaat atatatacca gctaagatac ctataagaaa gaaagaggaa cggaggaaac
     1561 ccttccttat cccccataat aatccgagaa gtataaaaat tacactgact atatcaatgc
     1621 taattttact accactcacg taatctcccg cctatatctg ccagtttatt ctttaatagt
     1681 tctatttttt cttctacctc ttcctgtgtt agtgttctat ctttggctct gagatttagt
     1741 gagaaggcaa gattaacagt cccttccggt atattgggac ctttatataa atcaaacaat
     1801 tcataatcct ccagggtatc tttaaatacc tcttccagaa tactggtaac ttctactgaa
     1861 ggcagtgtct ctgggactac aattgctata tctctcttta attttggaag tcgtgatggt
     1921 tctttcacct gtccaatcga gaaaaactcc agaggaaatt catctacatc catctcggcg
     1981 atatagattc tgggtagttt taatattctg gctacatctg gatgtaattc tcctatgaaa
     2041 cctgtgactt tctctcgata taaaatatta gctgttcttc ctggatgaag aacagagtaa
     2101 taagcaggga caaatgtcaa ttcatgctta aatagtttct ccaataatcc cttggtagaa
     2161 aaaaagctgg cattaacctc tttatcccag gaataaataa ttctccctga ttggacgata
     2221 cccagatgtt ccttctccat aaagctctct ccatccttga aaaatatctt gcctatctca
     2281 aagaggttaa aatcgttata tccccatctc acattgttga tagctaatga gagaagagat
     2341 tgggatagta gccatcttaa tactgattcc tcttctctaa gaggatttat aacacttata
     2401 ctgcttatat cgtctgcgta aaattctcta aagtacttat caaattgaac tggaacaaaa
     2461 ctggatgtaa taacttcact acatccgaga gaggtaattt tccttctcaa tatcttacgc
     2521 ttttcccatg ctggagatgt tgacactacc ccagtaattc tttcatgcag gatagatgga
     2581 accttatcat aaccaattat tcttactacc tcttcgtata gatcttcttc cagtgatatg
     2641 tctcttcttg
//
LOCUS       DAOTKU010000218         2646 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_31488_length_2646_cov_3.094172, whole genome shotgun sequence.
ACCESSION   DAOTKU010000218 DAOTKU010000000
VERSION     DAOTKU010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31488_length_2646_cov_3.094172"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(668..1897)
                     /locus_tag="P5310_05685"
     CDS             complement(668..1897)
                     /locus_tag="P5310_05685"
                     /inference="COORDINATES: protein motif:HMM:NF012870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HRU32885.1"
                     /translation="MVINVPKGVYTMKGKMSIIKQNASLYQKSKKKEKSIILDELTAI
                     LPLSRKHLAFLLRNTGKTIYIRGKKVKVDLELTQLSNRGRKKAYTEEVIKPLLELWRI
                     SGYISSKHLVKFIKLNEDKLSNFFDKLNPDVRERLLKISASTVDRLLKPLRDKVKLKG
                     RYKRNPFSSNLKKSIEVEPWFDKPRIPGYVEIDLVHHSGASDKGDFIYTFTATEITTG
                     WTELRALKNNAMVWTKKALKDIMDDMPIKVRALHSDNGSEFINAHIQKFCMEEGIEFT
                     RSRPYRKNDAPYVESKNWSLVRAYTGWRRYDTEEEEEVLGRLLELISLRHNLFIPTMK
                     LSIRERIGGKIRRQHSIDTPFNRVIEIDEVSKESKERLKAKRESIDLITLSLEIEKLG
                     EELDRIYERKIRRYNHE"
     gene            1952..>2646
                     /locus_tag="P5310_05690"
     CDS             1952..>2646
                     /locus_tag="P5310_05690"
                     /inference="COORDINATES: protein motif:HMM:NF012576.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRU32886.1"
                     /translation="MSILKKQTILGKIIFEERRTIDKIDFALYYVDMRLKTILGKEKP
                     VNIKDVAELAGVSPATVSRVINNRTWVKEETRIKVSKAIESLGYVPNNSARNLSRMKT
                     DIIGVIIANPHDSLYTDITFSEIIKGIGEIIDQEGYGLLLSKCNGRYINFKSLPNMVN
                     KRFIDGLILGGYPIEDRYIKALCSQISPLVVIGRYLREGEIYRILIDNVGGGFKATEH
                     LINLRYKRIGIIIG"
BASE COUNT          784 a          474 c          453 g          935 t
ORIGIN      
        1 taagagagat aatgagatta cattctgaaa atctttggat tataggtacg gttgggatgc
       61 catggatgat ggcagtggta aaaaataact tgagaaatgt acctgaagaa gcggctgttt
      121 ggtggccggc gtctccaggt gttacacgtc cagaacaatt ctttttcaag aaataagata
      181 gaggcggggg agaaaccccc gccttgtttg tattttaaga caaaaggaga aaactgtatt
      241 tttgtcgaat atcttgtagt gatttttgcc ttttcagatt tagatggtaa tgctaatttc
      301 tgactttgat atttcagcca gatagaatct tcctcaaatc tggaattata aaatcaaaat
      361 acgatatttt gccactacaa tgcctctgat agtttagatt gacgtctatt ccatcagagt
      421 tttgttgctt agatctgccc tggtccagac atattggacc ttcaaatgca aaaagggtta
      481 aagaaccttt aatttcgccc ttgaagattg atttgcttct tgagtctgca ataactttaa
      541 ttttatcata tatctggtac tgccaagatt ctttaataaa aagatgtata ccgaaggtta
      601 tttctagaag aagatgagtg acccccgtct ttcctcaaaa taaaatcctc ctgaaatatc
      661 tctaatatta ctcatggtta tacctcctaa tcttcctttc atatatacgg tcaagttctt
      721 ctcctagttt ctctatctct agggaaagcg ttattaggtc tattgactct ctctttgctt
      781 ttagtctttc cttactttct ttagacactt catctatttc tataacccta ttgaatggag
      841 tatctatgct atgttgcctt cttattttac cacctattct ttcccttatg ctaagtttca
      901 ttgttggtat aaataagttg tgccttaatg agattaattc taacagtcta ccaagcactt
      961 cctcctcttc ctctgtatca tatctcctcc atcccgtgta tgcccttact agtgaccaat
     1021 tcttactctc tacatatgga gcatcatttt ttctgtatgg cctagacctg gtaaattcta
     1081 tcccttcttc catacagaac ttctggatat gagcattgat aaactctgaa ccattgtctg
     1141 agtgtagtgc ccttaccttt attggcatgt catccattat atcctttaat gccttctttg
     1201 tccaaaccat tgcattgttc ttcaatgccc ttaattctgt ccatcctgta gttatttctg
     1261 tcgctgtaaa ggtatagata aaatctccct tgtcagatgc tccagaatga tgaactaggt
     1321 ctatctctac atatccaggt attctgggtt tatcaaacca gggctctacc tctatagact
     1381 tctttagatt agaactaaat gggtttcttt tatatctacc ttttaacttt accttatctc
     1441 ttaaaggctt tagtaacctg tctactgtag aggcacttat ctttagaagc ctttctctta
     1501 catcaggatt gagtttatca aagaagttag aaagtttatc ttcgttgagc tttataaact
     1561 ttacaagatg tttagatgaa atatatccag agattctcca tagttctagt agtggtttta
     1621 taacttcttc agtataagct ttctttctac ctctatttga gagttgagta agctccaagt
     1681 ctaccttgac cttctttccc cttatgtaaa tagtcttacc agtattcctg agaaggaacg
     1741 ctagatgttt tctactaagg ggtagtatag cagttaattc atcgaggata atagatttct
     1801 ctttcttttt agacttttga taaagactag cgttttgttt aattatagac atcttgcctt
     1861 tcatagtata tactccttta ggtacgttga tgaccatcct gtgacctcct ttaatataaa
     1921 tttcttatag gttacaggat ggttacttct tttgtcaatt ttgaagaaac aaaccatttt
     1981 ggggaagatt atttttgaag aacgtcggac tattgacaaa attgattttg cattatacta
     2041 tgtagacatg agactaaaaa cgattttagg aaaagagaag cctgtaaata taaaagatgt
     2101 ggcagaattg gcaggagtat ctccagccac tgtctctcga gtaataaaca ataggacttg
     2161 ggttaaagaa gaaactcgaa taaaagtttc taaggctatc gaatctttgg gctatgtacc
     2221 taataattct gctagaaatc taagtagaat gaaaacagac ataatcggtg tcattattgc
     2281 taatccccat gattctctct atacagatat tactttctca gagattataa agggtatagg
     2341 agaaataata gatcaggaag gatatggttt actcctttca aaatgtaacg gaagatacat
     2401 caactttaaa agtcttccca atatggtaaa caaaagattt atagatggct taatcttagg
     2461 aggatatcca attgaagata gatatattaa agcattatgc tctcaaatat cccctcttgt
     2521 agtaattgga agatacttaa gggagggaga aatatatcga attcttattg ataatgtagg
     2581 aggaggattt aaagctactg aacatcttat aaatcttaga tacaagagaa taggaattat
     2641 aattgg
//
LOCUS       DAOTKU010000219         2633 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_31714_length_2633_cov_4.577580, whole genome shotgun sequence.
ACCESSION   DAOTKU010000219 DAOTKU010000000
VERSION     DAOTKU010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31714_length_2633_cov_4.577580"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            186..1268
                     /locus_tag="P5310_05695"
     CDS             186..1268
                     /locus_tag="P5310_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HRU32887.1"
                     /translation="MEYFNGLDMNLGNLPRLSKAITRSISPENFTGEKGKGGMATDGT
                     GALCARDLGRGWKISPSVRIKAGETFTLADIQDSGSIQHIWMTPTGHWRHSILRIYWD
                     DSEIPSVECPVGDFFACGWGQYAQVSSLAVCVNPGSGLNCYWPMPFRKRCRITMQNRA
                     QEDMVLYYQIDYTLTEVPEDVAYFHAQFRRVNPLPYKQVYTILDNVTGEGHYVGTYLA
                     WGVHNNGWWGEGEIKFYMDGDDEFPTICGTGTEDYFCGSYDFDNPMTGEYQEFTTPYS
                     GLAQVIRPDGHYKSQMRFGMYRWHIMDPIRFQHDIRVTIQALGWMSGRRYLPLQDDIA
                     SVAYWYQSLPTNPFPPLPNPDYLEII"
     gene            complement(1482..>2633)
                     /locus_tag="P5310_05700"
     CDS             complement(1482..>2633)
                     /locus_tag="P5310_05700"
                     /inference="COORDINATES: protein motif:HMM:NF018682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HRU32888.1"
                     /translation="AYSEKKAGEIDEEVVTRLLKYVDPSLLKIISRSDSTLRGHFGLE
                     VKVLLDSLGPFDGVIVVPYFKEGGRFTLFDTHYVLQGEDLVEAHRTEFASDPVFSFKN
                     SYLPAWIEEKSGGIWKEEDVISISIDDIRIGGIDRVYEVLKDVRDTKPVIVNCLDDED
                     LEVFILGLLKAESEGKRFLYRTAASFVKIRAGIEDRETVRSFLMKKGIIIVGSFVKKT
                     TEQLEALLEDFPLDRVELKISKILSEERTDYLKGVVERMNEALLQDKSIVVYTERDYA
                     IPDLGKEEQLRSGETISLFLADIVKNLKVSPDFLIAKGGITSYIVAKEGLGVKVADVI
                     GQIAPGVPVWKLRGGCRFPDLFYIVFPGNVGDRLTLSRIFSTLIDISKA"
BASE COUNT          746 a          577 c          549 g          761 t
ORIGIN      
        1 tttctggtgg tccttagact cctcggaaac ctgcggcatt tatagtatag attaggctgg
       61 aaaatctgtc aataccccat aggggtatac cggattatca gcagatattc agagtgacca
      121 aaatatgata tcattagaca aagctcttgt ctttaactga taaattaaat tctgggggtg
      181 atattttgga gtattttaat ggtctggata tgaatttggg gaacctgccg aggctttcaa
      241 aggctatcac tcgatctata agtcctgaga atttcactgg agaaaagggt aagggtggta
      301 tggctaccga tggtacaggg gcactatgcg caagagatct ggggagagga tggaagatat
      361 ccccctcggt taggataaaa gctggggaga catttacact ggcagacatc caagactcag
      421 gctcgataca gcatatatgg atgacgccta ccggtcactg gaggcacagt atactcagaa
      481 tctactggga tgattcagaa atcccatcgg tggaatgtcc tgttggcgat ttttttgcct
      541 gtggttgggg acagtatgct caggtttctt cccttgctgt atgtgtaaat ccgggtagcg
      601 gtcttaactg ttactggcct atgcccttca ggaaaagatg caggattacc atgcagaaca
      661 gagcccagga agatatggtg ctgtactacc agatagacta tacattaact gaggttccag
      721 aagatgttgc atatttccat gcccaattca ggagagtaaa tcccctgcct tacaaacagg
      781 tatacactat tctggataat gtaacaggtg aaggacacta tgtgggcaca tatcttgcct
      841 ggggtgtgca taacaatggc tggtggggag agggtgagat aaagttttat atggatggtg
      901 acgatgaatt ccccacgatt tgtggaacag gtactgagga ttacttctgc ggatcttacg
      961 actttgataa tccaatgacc ggagaatatc aggagtttac aacgccatac agtggtcttg
     1021 cccaggtgat acggccggac ggacactaca aatctcagat gaggtttggg atgtacaggt
     1081 ggcatataat ggacccgata cgcttccagc atgatatcag ggttactata caggctctgg
     1141 gatggatgtc agggagaaga tatcttccgc ttcaggatga tatagcttct gttgcatact
     1201 ggtatcagtc acttcccaca aatccatttc cacctctacc taatccagat tatctcgaga
     1261 taatatagag aacctgtcag gataccgatt ttgcaggggt tcgagttttt gaacccctgc
     1321 aattccaggt atattgttgt cctctatttt cattatactg atttaaagct cgaaaaggca
     1381 gagacgattt tattaaagag aatggaactt tcaaaatatg taaaaatcca caagaaaatg
     1441 ggtcgttcag gcacggaagc caagtcgata ctaggaatct tctaggcttt agaaatatca
     1501 attaatgtgc tgaagattct gcttaatgta agtctatcac ccacatttcc tggaaagact
     1561 atatagaaaa gatccgggaa tctgcatcct cccctgagct tccacaccgg gactccagga
     1621 gctatctgtc caataacatc tgctactttt acacctaacc cctcttttgc aacaatataa
     1681 gatgtaatcc cccctttggc tataaggaag tctggagaga ccttcaggtt tttcactata
     1741 tctgccagaa aaagagaaat cgtctcacca gatcttagct gttcttcctt gcccaggtcc
     1801 ggaatagcgt aatccctttc agtatatacc actatagatt tgtcctgtaa aagagcctca
     1861 ttcatccttt ccaccacgcc cttaagataa tctgtccttt cttcagagag aatctttgag
     1921 atcttgagtt ctaccctgtc caggggaaag tcctcaagaa gagcttctaa ctgctctgtc
     1981 gttttcttta caaaagaacc aactattatg ataccttttt tcatgaggaa agaccttaca
     2041 gtttctctat cttcgatacc ggctcttatc tttacaaaag aagcggctgt tctatataaa
     2101 aatctcttcc cttcagactc tgctttaagc agtcccaaaa taaatacttc caggtcttca
     2161 tcatcaagac aattcactat aactggtttt gtatctctta catccttcag aacttcgtaa
     2221 accctgtcga ttcctccgat ccttatatca tctatcgata tggaaataac atcctcttcc
     2281 ttccatatac ctccagattt ctcttcgatc catgcaggta gataggaatt tttgaatgaa
     2341 aatacagggt cactggcaaa ttcagttcta tgagcttcta ccaggtcttc gccctggagt
     2401 acataatggg tatcaaagag agtaaatcta cccccttctt taaagtatgg aacaactata
     2461 accccgtcaa atggacccaa tgaatctagc aagactttta cctccagacc aaaatgcccc
     2521 ctcaatgtgg agtcactcct gcttataatc ttcagcagag aagggtcaac gtatttcaga
     2581 agtctggtta ccacctcttc atcgatctct ccagcctttt tttcagagta tgc
//
LOCUS       DAOTKU010000220         2615 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_31993_length_2615_cov_2.637109, whole genome shotgun sequence.
ACCESSION   DAOTKU010000220 DAOTKU010000000
VERSION     DAOTKU010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31993_length_2615_cov_2.637109"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            51..>2615
                     /locus_tag="P5310_05705"
     CDS             51..>2615
                     /locus_tag="P5310_05705"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HRU32889.1"
                     /translation="MFSLENPNPIELEGWDAWVKGNHRKVIQNWERLIADNPDCYSSL
                     SYWYLLSELYDEEGLYREAISFIDSIIDSVKSPDLLSSLYWEKALNLLKLGEYKSAED
                     CISRLGLVNEWLIIGPFDNVGRSGFYKVFPPEEGIDLNKIYTGKSGMEVKWFPSKTTY
                     GVMELGNLLYPSSSAVAYALTFLYSDSEQEVFIRSGSTGALAIWVNDTVVYSQDTYRT
                     MYMDQDVIKVRLHKGWNKLLVKVCEDEGTWKFVLRVTDDNGNSIPTIKYSLTPVEFTK
                     GSEYKLLEEDTPQDYWSLMYAGIIYRKRGLWDKAEKSFLKAIDIFDSALPRYFLGITY
                     LDEGESDRGINELKRVISSIPDFVKAYVDLGWNRYGKGLYEEAIRFFQDSIKYSTDYP
                     QIYSYLAWTYLARGWIKDAEDVISEALSINQNHPLSNYVQGLIYEQRGWYDDAVNIYK
                     KLISSNRTFVSASSNLSSIYKNSGRYEDAISVTKERLEKEPYNITLYLYLADLYTRSK
                     MFVEAISLLESASEISPFYPAIYEYLGDLYYELEQKEQAIIYWEKAIEIEPGNVALKD
                     YLSFIKEESYTLADISNLITSTPTQKDYPDASAIIILDSGERIVKPDGTSSLTYHKVI
                     KILNERGREMYGEVYIPYNLSGERVKILRARTIKPDGTEVEATAINDVSLAEGYHLYS
                     DAMAKVISMPALENNAIIEYAYTVDELGRGPLGRHFQDEFLFQDIDPMMISRYSLTVP
                     KGMEIYYKVYNGDISPEIEEKGNSVTYIWEAKNTPQIIQEDYMPPLADIVPRLEITTF
                     KDWQEVAGWYYDITTPKTKPDEAIKAKVIELTKDKKTLEDKVKAIYYFVTNEVRYVG"
BASE COUNT          820 a          456 c          616 g          723 t
ORIGIN      
        1 ttttattact ggttattcca aacatctctt ttgcccagga gaaagggcta atgttttcct
       61 tagaaaatcc caaccccatc gaacttgaag gctgggatgc ctgggtaaag gggaatcatc
      121 ggaaggttat acaaaactgg gagaggttaa tagctgataa tccggattgc tactcttctc
      181 tgagttactg gtatctgctc agtgaacttt acgatgagga aggattatac agagaagcta
      241 tctcctttat agatagcata attgacagtg ttaaaagtcc agatttattg tcctccctct
      301 actgggagaa ggctttgaac ttactgaaac ttggcgaata caaatctgca gaggattgta
      361 tctccaggct tggtcttgtc aatgaatggc tcataatagg tccatttgat aatgtaggaa
      421 gatcagggtt ttataaagta ttccctccag aagagggaat cgaccttaat aagatatata
      481 caggcaaaag tggaatggag gttaagtggt tcccatcaaa aaccacttat ggtgttatgg
      541 aattaggaaa tctactgtat ccatccagtt ctgcggttgc ctatgctctg acatttttat
      601 acagtgattc tgaacaggag gtctttatca ggtctgggag taccggcgct ttggctatct
      661 gggtgaatga tacagtggtt tactctcagg atacatacag gaccatgtat atggaccagg
      721 atgtgataaa agttaggctg cacaagggat ggaataaact tcttgtcaag gtatgtgaag
      781 atgaaggtac ctggaaattt gtcctcagag ttacagatga taatggcaac agtatcccca
      841 caataaagta ttctctcact cctgtggaat ttactaaggg aagtgagtat aaacttctgg
      901 aagaagatac cccccaggat tattggtcgc tgatgtatgc tggaataatc tataggaaga
      961 gaggactctg ggataaagca gaaaagtcct tcctcaaggc tatagatatt ttcgattccg
     1021 cccttcccag atacttcctt gggataacat atctggatga aggagaatca gatagaggga
     1081 taaacgagtt aaaaagagtc atatcttcta tccccgactt tgttaaggct tatgtggatc
     1141 tgggatggaa caggtatggt aagggattat atgaagaggc tattcggttt ttccaggata
     1201 gtataaaata cagtactgat tatccacaga tctattctta tctggcatgg acttatctgg
     1261 caaggggatg gataaaagat gctgaggatg ttatatctga agcactgagt atcaatcaga
     1321 atcatcctct cagtaattat gtacaggggc tcatctatga gcagaggggg tggtatgatg
     1381 atgcggttaa tatatacaag aagcttatct cctcaaaccg tacatttgtc agtgcttcca
     1441 gcaacttgag ctcaatatat aagaatagtg gaaggtatga agatgcaata tcggtaacaa
     1501 aggaaagact ggaaaaggaa ccgtataata taacccttta tctatatctg gcagacctat
     1561 atacgaggtc taagatgttt gttgaagcaa tctccctcct tgagagtgcg agtgagatct
     1621 caccatttta tcctgcaata tatgaatatc tgggagatct gtactatgag ctggaacaga
     1681 aagaacaggc aataatatac tgggaaaagg ctatagaaat agaacctgga aatgtagctc
     1741 taaaggacta tctgagtttt attaaggaag agagctatac ccttgcagat atctcaaatt
     1801 tgattaccag tacccctacc cagaaggatt atccagatgc ctcagccata attatccttg
     1861 atagcggaga aaggatagtt aaacctgacg gaacatcctc tcttacgtat cataaggtga
     1921 taaagattct gaatgaaaga ggaagagaga tgtacggaga ggtttatatc ccatataacc
     1981 tttcagggga gagggtgaag atactcagag ccaggactat aaaaccagat gggactgaag
     2041 tggaggctac tgctataaat gatgtgtcac tggcagaggg ctaccatctt tattctgatg
     2101 ctatggctaa ggtaatctct atgcctgccc tcgagaataa tgccatcata gagtatgcct
     2161 ataccgtaga tgagcttggt agaggtcctc tgggaaggca ctttcaggac gaatttctct
     2221 tccaggacat tgatccaatg atgatatcca gatattccct gaccgttcca aaggggatgg
     2281 agatttatta taaggtatat aatggcgata tatctcctga gatagaggaa aaggggaatt
     2341 ccgttacata tatatgggag gcgaaaaata ctcctcaaat tattcaggag gattatatgc
     2401 cacctctagc tgatattgtt cccagactgg agataactac ttttaaggac tggcaggagg
     2461 tggctggctg gtattatgat ataaccaccc ccaaaaccaa acctgacgag gctataaaag
     2521 ctaaggtgat tgagcttaca aaagataaga agacgcttga ggataaggta aaggcgatat
     2581 actattttgt caccaatgag gtcagatatg tgggg
//
LOCUS       DAOTKU010000221         2614 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_32002_length_2614_cov_3.536147, whole genome shotgun sequence.
ACCESSION   DAOTKU010000221 DAOTKU010000000
VERSION     DAOTKU010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32002_length_2614_cov_3.536147"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            446..2131
                     /locus_tag="P5310_05710"
     CDS             446..2131
                     /locus_tag="P5310_05710"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRU32890.1"
                     /translation="MGGDKDFELELKKIYQAIEQASDWVVITDRDGNIEYANRVVENI
                     TGYTRNELIGKNPRVFKAGLHDREFYKKMWDTILSGETFYGSIVNRKKNGETFELFHT
                     ITPLFDENGNITHFISTAKDISYEKKLREKIEGISFIDLVTNLYNRSFFINRITQAII
                     EARGRDVHLAIIGLDIDSFTSINYQYGYQMGDMILKEVGNILKSIVPDSYTVARIGGD
                     DFGILCIDIKDTRKLDSLVGKIIERFSDPVVVAGLSIPIDVDMGIAVYPQDGDNTHIL
                     VANMGLALNVAKRDNLKKSKYVFFSEELNKRAERYFTVQEHVLREMRKDTLIIRYQPY
                     FDIKTMRPVGMEALSRCSHVDAKILTVEELFSFLEESGMIVSVGEWIIERIVEDIKRW
                     RGIKMPVSINISPVQFKDRYFVERLEDNIRSSGINPELIGIEMTERVFIEDMETVKEK
                     LTRLKSMGMSISIDDFGKGYSSLIYLKRLPIDNIKIDISFIRDIDNDPDDASIVSAII
                     SMAHELGIKTIAEGVEDKEQLKILRLLRCDMVQGYLLSLPLLPEELEKQFNLL"
     gene            complement(2137..>2614)
                     /locus_tag="P5310_05715"
     CDS             complement(2137..>2614)
                     /locus_tag="P5310_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRU32891.1"
                     /translation="VSELACRIARKMGIAEDIIERIRIAGLLHDIGKVSVPGEILNKP
                     SKLNSLEFEIVKIHSRAGYDIVKKIDLFSDMAEIILQHHERLDGSGYPRGLKNGEILL
                     EARILAVADVVEAMSSHRPYRPALPEEEIKSELMSNKGKLYDPEVVDAYLATVPLR"
BASE COUNT          838 a          447 c          576 g          753 t
ORIGIN      
        1 tatgctgact atccacaatg tttatccctg tttccaaatt tttattccag gatagcgcca
       61 tagttccctc catccaacaa ttttttatta aatattatta ttattattat cttaaattat
      121 atattttcca ggatatataa gtcaaattac tggtcatctg agagaagagt ccctactttt
      181 gataattcag atgagataga aatattctga gtctggcagg ctgatgaatc gagtcaagat
      241 ttctctctaa catctccatg gacatagaga gtgattcaga ttataataat cttgaaaaat
      301 caaaaggcga tagaagatga gagagacaga ttttatctta tggaaagagg ataggtatta
      361 tcattttccg tagattctgg atataataga tatattctac aaatcctgcc ggtacctgaa
      421 tagctggaaa aggagtgtaa gagacttggg tggagataaa gactttgaac ttgagcttaa
      481 aaagatatat caggctatag aacaggcatc agattgggtg gtcatcaccg atagagatgg
      541 aaatatagaa tatgcaaaca gggtggtgga gaatatcact gggtatacca ggaatgaact
      601 cattggtaag aatccgagag tattcaaagc aggtctgcac gacagagaat tctataagaa
      661 gatgtgggat accatcctgt ctggtgagac attctacggc tctatagtca acaggaagaa
      721 gaatggcgag acatttgaac tgtttcacac cataacaccc ctgtttgacg aaaatggaaa
      781 tataactcat tttatatcga cagctaaaga tatatcatac gaaaaaaaac tcagggaaaa
      841 gatcgaaggc atctctttta tcgatctggt tactaacctt tacaatagaa gtttctttat
      901 aaacaggata actcaggcaa ttatagaagc caggggcagg gatgtccacc ttgccattat
      961 aggtttagat atagacagct ttaccagtat caactatcag tacggatatc agatgggaga
     1021 tatgatttta aaagaggtcg ggaatatact gaagagtata gttcctgata gctatacagt
     1081 ggcaagaata ggtggagatg actttgggat actttgcata gatataaaag atactagaaa
     1141 gctagattcc ctggttggta agattataga gaggttctca gatccggtag tggtggcagg
     1201 tttatcgata ccgatagatg tagatatggg tatagcggtg tacccgcagg acggcgataa
     1261 tacccatata ctggttgcca atatgggact tgcattgaat gtagcgaaga gagataacct
     1321 gaaaaagagt aaatatgtat tcttctcaga ggagttgaat aaaagagcag agagatactt
     1381 tactgttcag gaacatgttt taagggagat gagaaaggat acactgataa taagatacca
     1441 gccatacttt gatataaaga ctatgagacc tgttggtatg gaagcccttt caagatgttc
     1501 tcatgtagat gcaaaaatat tgacagtaga ggaactcttt agtttcttag aagagtcagg
     1561 gatgattgta tctgtaggtg aatggataat agagaggatt gttgaggata taaagagatg
     1621 gcgggggata aagatgcctg tctccataaa tatatcccct gtccaattca aggatagata
     1681 ctttgttgaa agacttgagg ataatataag gtcttcaggg ataaacccag aactgatcgg
     1741 tatagagatg acagagaggg tattcataga agacatggag acggttaaag aaaagctgac
     1801 caggctgaaa tctatgggga tgagtatatc tatagatgat tttggcaagg gttactcgtc
     1861 acttatctat ctgaagaggc tcccgataga taatatcaag atagatatat catttataag
     1921 agatatagac aatgaccctg atgatgcaag tatagtctct gccataatat ctatggctca
     1981 tgagcttggg ataaagacga tagctgaagg ggtagaggat aaagaacaac tgaagatctt
     2041 aagactctta agatgtgata tggttcaggg atatctcctc tctcttcccc tcttaccgga
     2101 ggaattagag aaacaattta atctattata ggaatattac cggagtggaa cagtggctag
     2161 atacgcatct accacttccg gatcgtacag ctttccctta ttgctcatca gttcgctttt
     2221 gatctcttct tcaggcagtg ctggtcggta tggtctgtga gagctcatcg cctccacaac
     2281 gtctgcaaca gcaagtatcc ttgcctccag cagtatctct ccgtttttca gcccccttgg
     2341 ataacccgag ccatcaagtc tctcatggtg ttgcaggatt atctctgcca tatcggagaa
     2401 aaggtcgatc ttcttaacta tatcgtaacc agcccgtgag tgaatcttta ctatttcaaa
     2461 ctccagagaa ttcaatttcg atggtttatt gagtatctct cccgggacag atacctttcc
     2521 tatatcatgg aggagtcctg ctatccttat tctctctatt atatcttcgg ctattcccat
     2581 cttcctggcg attctgcaag ctaactctga aaca
//
LOCUS       DAOTKU010000222         2586 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_32433_length_2586_cov_2.801659, whole genome shotgun sequence.
ACCESSION   DAOTKU010000222 DAOTKU010000000
VERSION     DAOTKU010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32433_length_2586_cov_2.801659"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="P5310_05720"
     CDS             complement(<1..279)
                     /locus_tag="P5310_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRU32892.1"
                     /translation="MLFYEILKMAFDSFRLNKLRTVLTALGIVIGVAAVVALMALGEG
                     TRASIEGQFSSLGANLLIVRPGGRVGGPGLVRTGPRPSLKYEDYESLLK"
     gene            complement(293..1588)
                     /locus_tag="P5310_05725"
     CDS             complement(293..1588)
                     /locus_tag="P5310_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRU32893.1"
                     /translation="MKKRWLILVVIFLAVAAGAYAFISSRNKENPQGPPIETYTVTSG
                     DLIVSASGSGILEARETVDITSEIGGTVTWVVEEGSKVKKGDLLCKIDPHDYEVALSR
                     AELSYKNALLKLDQTRLNLEEQKKQLQKNLQDAKISRDNAYIEYQRAKRQLNDSETLY
                     KANAISLSELQTDKDSYNKALNAYNQAVENLRLVESSLSNQIVQLEKDASLAEISVEQ
                     AKIDLDNAKLNLSKTVITAPIDGIVANVSVKKGGQNIAKETNLMSILNTSVMRVSLEI
                     DEVDIPKVSVGLPVRVTCEAFGDREFQGKVSLISPSARISNNIAIFDVRVDIPNSTGE
                     LRAGMTAEGEIILKEDYNVILVPIKAVRRTEGKAYVNVLNDKGEIEVREVILGEDDGV
                     DIVVKSGLQEGDKIVTSAITSSSGTPGPTPGGPVIRIGR"
     gene            complement(1602..2294)
                     /locus_tag="P5310_05730"
     CDS             complement(1602..2294)
                     /locus_tag="P5310_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRU32894.1"
                     /translation="MEELILIQDIYKVYKMDSVETVALNGVSLNVNKGDFKAIMGPSG
                     SGKSTLMHIIGCLDRPSSGKVLWEGKDVSTLSDDELAMIRNRRIGFVFQSFYLLPRYT
                     ALQNVELPLIYRGLTAKERREKARRLLELVGLEDRIDHRPTQLSGGQQQRVAVARALA
                     VDPLILLADEPTGNLDSKSSHEIMDLISNLHNQGLSIVLVTHEYDIAGYANEIVKMAD
                     GKIIDIEKKSKG"
     gene            complement(2331..>2586)
                     /locus_tag="P5310_05735"
     CDS             complement(2331..>2586)
                     /locus_tag="P5310_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32895.1"
                     /translation="NNDTGIGLQTFKYSREERFKYPQELFLNAVESTKSLPKTILVRE
                     EDSYEALIDFTRQVGIGIEVPENIEILDSLRDLFLEHLRY"
BASE COUNT          720 a          652 c          395 g          819 t
ORIGIN      
        1 ctttaacaga gattcataat cctcatattt taaagaaggt cttggacctg tccttacaag
       61 acctggtcca cctaccctgc cacccggtct aactataaga agattagccc caagggaaga
      121 gaactgtccc tctatacttg cccttgtacc ttcccccagt gccataagag ccacaactgc
      181 cgctacccca attactatac ccagggcagt gaggactgtt ctgagtttat tgagtctaaa
      241 actatcaaaa gccatcttta atatctcata aaagagcata cttattctcc ccttatctgc
      301 caattctaat aacaggacca ccaggagtag gaccaggagt tcccgagcta gaggtaatgg
      361 cagatgttac tatcttatca ccctcctgta atccactttt cacaacaata tctactccat
      421 catcttcacc caatattacc tcccttacct ctatttcccc tttatcgttt aaaacattca
      481 cataagcctt accctctgtt ctccttaccg cctttatagg gactaaaatc acattatagt
      541 cttctttcag aatgatctcc ccctcagcag tcataccagc tctgagttct ccagtagaat
      601 ttggaatatc aacccggaca tcaaaaatag cgatgttatt ggatatcctt gccgagggag
      661 aaattaaact cactttacct tgaaactctc tatctccaaa agcctcacag gttaccctta
      721 caggtaaacc taccgatacc ttaggtatat caacctcgtc tatctctaaa cttaccctca
      781 ttacactggt attaagtatg gacataaggt ttgtctcttt tgctatattt tgtccaccct
      841 ttttaacaga aacattggca actataccat ctataggagc agtgataact gttttactta
      901 aatttaactt cgcattatcc agatcaatct ttgcctgctc aacagaaatt tcagccagag
      961 aggcatcctt ctctaactgt actatttgat tagatagcga agattcaacc aaacgtagat
     1021 tttccactgc ctggttgtat gcattcaggg ctttgttata actatcttta tcagtttgaa
     1081 gttcactgag agagatagca ttagccttat aaagggtctc cgaatcattc agctgtctct
     1141 tagccctctg gtactctata taagcattat ccctgctaat cttagcatcc tgaagatttt
     1201 tctgtaattg cttcttctgc tcctccagat taagcctggt ctggtccagc tttaagagag
     1261 catttttata actgagttct gctcgagaga gagcaacttc ataatcatga gggtctatct
     1321 tacagagaag gtcacccttt ttaacctttg acccctcttc cacaacccag gtaaccgttc
     1381 cccctatctc acttgttata tcaactgtct ctcttgcctc caggattcca ctcccactgg
     1441 cagagactat taaatcgcca ctcgttacag tataggtttc aattggaggt ccctgaggat
     1501 tttctttgtt tctggaggaa atgaaggcat acgcccccgc agcaactgcc agaaaaataa
     1561 caactaatat taaccatctt ttcttcatct ttatctctcc cctaaccttt ggactttttt
     1621 tctatatcta ttatctttcc atcagccatc tttactatct cattggcgta accagctata
     1681 tcatactcgt gggtgacaag gactatgctc aatccctggt tatgtaaatt tgatataaga
     1741 tccataatct catgactgga cttactgtct aaattcccag taggctcatc tgccagaagt
     1801 attaacgggt ctacagctaa tgccctggct actgcaaccc tctgttgctg tcccccagat
     1861 agctgggtag gtcgatggtc aattctatcc tctaacccta ctagctctaa aagtctcctt
     1921 gctttttctc ttctctcttt agctgtaagt cctctatata tgagaggaag ctcaacattc
     1981 tgcagagcgg tataccgtgg caacagatag aaactctgaa agacaaaacc tattcttctg
     2041 ttccttatca tagcaagctc atcatcagat agtgttgata catctttccc ttcccagagg
     2101 acctttccag aactaggtct atccagacat cctataatat gcatcagtgt agatttgcct
     2161 gatccggatg gacccattat agccttaaaa tcccccttat ttacgttaag acttacccca
     2221 tttaaagcta ccgtctcaac actatccatt ttatacacct tatatatatc ctgaataaga
     2281 atcaattctt ccataacttc taattagacg caaggggaga taattttatt ctaatagcga
     2341 agatgttcca aaaaaagatc cctcaggcta tcaagtatct caatattctc aggtacctct
     2401 atacctatac caacctgtct ggtaaagtct atcaatgcct cataagaatc ttcttctctt
     2461 accagaatag ttttgggtag agattttgta gactcaacag cattcaggaa taactcctgg
     2521 ggatacttaa atctttcttc tcttgaatat ttaaaagttt gaagaccaat cccggtatca
     2581 ttatta
//
LOCUS       DAOTKU010000223         2585 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_32452_length_2585_cov_3.202767, whole genome shotgun sequence.
ACCESSION   DAOTKU010000223 DAOTKU010000000
VERSION     DAOTKU010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32452_length_2585_cov_3.202767"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            480..2156
                     /locus_tag="P5310_05740"
     CDS             480..2156
                     /locus_tag="P5310_05740"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRU32896.1"
                     /translation="MIFSVGITVSTVASAFIMGRIVDSAISGDMSGLLDNLYMILVIM
                     AVHTTSSLFHNYFKGRFAEYALFDLRERTAKHIGKLSIPCIDKHHSGELISSLTNDIV
                     VVQNFLQNNLGNILVEPMLFIISLFYLCSISWNLTLFSISIAPILMFLVMKMSKPIER
                     LTGEQQEFLSEVNTTAQDSISGLDVVKSFNLENTMEEKFSYRVDRAVSRGLEIALTQA
                     ILNPIGTIMGMSPFILTFLYGGYLVINGAFSFGNLISFIQVLNYVVNPLTSFPRLLST
                     LRSAVSALSRIFDVWELELEREDGDAFPRREDVPVISFKDVSFSYNTSGEVVSGLNLE
                     VKNGETIGIVGPSGSGKSTIVKLILGFYKPTRGDIYLYGERLMDWKLDSARSLISLVS
                     QDTYLFPESIYNNIAFGKKNATRDEVINASRLAGIHEFIESLPQGYDTFVGERGVRLS
                     GGERQRIAIARAFLKDTPILILDEPTSSLDADSEKRIQESLEKLMAGRTTIVIAHRLS
                     TVMMSDRIIVMNQGKIIDVGTHSELLERDSLYRQLYMNQINESKQEDVRR"
     gene            2153..>2585
                     /locus_tag="P5310_05745"
     CDS             2153..>2585
                     /locus_tag="P5310_05745"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HRU32897.1"
                     /translation="MRRFFKENILFKLLSLDKPRIFPYTIGLIGNSFSALAFNYLAAI
                     GIKHLADGSVNKDIEQLLYGIRFMGIGFLIFSIVFSILGYLFDSSVKKMTGNIRKRLF
                     SHTLRLPMGYIESKHSGDIISRLTNDVQIAENAYSWQLLMPM"
BASE COUNT          836 a          421 c          584 g          744 t
ORIGIN      
        1 cctctccctt gacgggagag ggcaggggtg agggtgaaag gaagaagatg cccccacctc
       61 tatcctcccc cgaggtgggg gaggaaggat aagggcatgg ggttttatca taaaagacga
      121 gattgtaggg gcaattcatg aattgcccct acagggatat aaaaaatgtt attgccagaa
      181 ataagatagg tataggtacg ataaatcgtg cccaatgatt cagggtttga tttatcaaac
      241 ccctacaatg atattatcat taaaaatata acgattacca tcaatattta tattgacaaa
      301 tttaaaaaat tttatataat cttttaaaga tatcaattta gaataaaatt tatcgaaggt
      361 gagttacgga tagggtcttt aaaaagaaat atagagatga gaatgagata aatagctgga
      421 agataatctc tcaatttatc cattttacca gaactcatcg atgggatcta atactgagta
      481 tcatcttttc cgtaggaatt acagtctcta cggtagcttc ggcattcata atgggaagga
      541 tagtggattc ggctatatcg ggagatatgt caggattact ggataatcta tatatgatac
      601 tggtaataat ggcagttcat accacatcga gcctgtttca taattacttt aaaggaagat
      661 ttgcagagta tgcactgttt gatctgaggg aaagaacagc taaacatata ggtaaactct
      721 cgataccctg tatagataaa caccactcag gggaactgat atccagtcta actaacgata
      781 ttgtcgtggt gcaaaacttc ttacagaata acctgggaaa tatcctggta gaaccaatgc
      841 tatttatcat atctctattt tacctatgca gcataagctg gaatttaacc ctattttcaa
      901 tatcgatcgc tccaatcctt atgtttcttg ttatgaagat gagtaagcct atagaaagat
      961 taacagggga acagcaggag ttcctctctg aggtaaatac cactgcacag gatagtatat
     1021 ctggattaga tgttgttaaa tcgttcaatc tggaaaatac tatggaggaa aaattttctt
     1081 acagggtaga tagggctgtc tctagaggtc tggagatagc tttaacgcag gctatcctca
     1141 atccaatagg gacgattatg gggatgtctc catttatact gacctttctc tatgggggat
     1201 atctcgttat aaatggggct tttagttttg gcaatctgat atcctttatt caggtattaa
     1261 actatgtcgt aaatccattg acctcatttc cgaggttact ctccacatta aggtctgcag
     1321 tctctgccct cagtcgtata tttgatgtat gggaactgga gctagaaaga gaggatgggg
     1381 atgccttccc aagaagagaa gatgtaccgg tcatatcgtt taaagatgta tcattctctt
     1441 ataataccag cggagaagtg gtctcagggt tgaatcttga ggtaaaaaat ggggaaacta
     1501 tcggtatagt tggtccaagt ggaagtggaa agagtaccat agtaaagttg atattaggct
     1561 tttataaacc aaccagggga gatatatatc tgtatggtga aaggttaatg gactggaagc
     1621 tagattctgc cagaagcctt atctctctgg tctctcagga tacatatcta tttccagaga
     1681 gcatatacaa caatatagcc ttcggtaaga agaatgctac cagagatgag gttataaatg
     1741 cgtccagact ggcagggatc cacgaattta tcgagagcct gccgcaaggt tacgatacct
     1801 ttgtaggaga aaggggtgtg aggctctcag gtggtgaaag acagaggata gcgatagcca
     1861 gagcatttct taaggataca ccaatattaa tactggatga accaacctcc tcactggatg
     1921 cagattcgga gaaacggata caggaatctt tagagaagct tatggctgga agaaccacta
     1981 tcgtaatagc gcacaggtta tctacagtaa tgatgtctga taggataata gttatgaatc
     2041 aggggaaaat aatagatgtg ggaacgcata gtgaattgct ggagagagac agcctgtata
     2101 gacagctcta tatgaaccag ataaatgaaa gcaagcagga ggatgttagg agatgaggag
     2161 gtttttcaag gaaaatatac tatttaagtt attatctctt gataaaccca ggatattccc
     2221 gtatacaatt ggtcttattg gtaacagctt ctctgccctg gctttcaatt atcttgccgc
     2281 tatagggatt aaacaccttg cagatggaag cgtaaataag gatatagaac agttgttata
     2341 tgggataagg tttatgggga taggattctt aatattcagt atagtttttt ctatattggg
     2401 atatctcttt gactcttctg taaaaaagat gactggaaat ataagaaaaa gacttttttc
     2461 ccatacattg agactaccta tgggttatat agagagtaaa cacagcgggg atatcatatc
     2521 aagattaaca aacgatgtac agatagcaga gaatgcctat agctggcagt tacttatgcc
     2581 tatga
//
LOCUS       DAOTKU010000224         2560 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_32843_length_2560_cov_3.947305, whole genome shotgun sequence.
ACCESSION   DAOTKU010000224 DAOTKU010000000
VERSION     DAOTKU010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32843_length_2560_cov_3.947305"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="P5310_05750"
     CDS             complement(<1..656)
                     /locus_tag="P5310_05750"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRU32898.1"
                     /translation="MDLVTRDWHLFSIRGEHFLFNSLGSLYQLDEEARSAMDTLYRGG
                     SLDISEDVYGEIEEILSSYKPLEIYEPEDLRLKSLCLNVSHSCNMSCSYCFATHNLSY
                     RMSLMPLEIACRAIDFLIDSSKDIKNIEIDFFGGEPLLNLEVVKNATIYGKTRALNSG
                     KNLRFTLTTNGLLLDSKTLDLLDELEISIVLSLDGGEDTHNKFRRLLNGGGSFKTILP
                     "
     gene            complement(644..811)
                     /gene="scfA"
                     /locus_tag="P5310_05755"
     CDS             complement(644..811)
                     /gene="scfA"
                     /locus_tag="P5310_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014453942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HRU32899.1"
                     /translation="MKKHIRVILDSPIAKKEEIEKCQECQTPCRSACKTSMTVGNLVC
                     EVKKSVGKWTW"
     gene            940..1599
                     /gene="phoU"
                     /locus_tag="P5310_05760"
     CDS             940..1599
                     /gene="phoU"
                     /locus_tag="P5310_05760"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HRU32900.1"
                     /translation="MEDNTYLDLIKSLTLEMLSVSKKNIKDGIDSLVNKDEILADRVV
                     EGDKYVDELELKIEDMCIKSLRNLDKEEDIRFISGIWKIITDAERIGDYATHVADSAK
                     VLASKPTLKPLIDIPAMAENVVDMISRCESALESYDLSYIDSIWELDREIDALYDQVF
                     RELLSYILETPRLITNVIYLTQVARYLERAGDHVTNIGERIFYIVTGKRIKRGNIQSE
                     S"
     gene            1618..2430
                     /locus_tag="P5310_05765"
     CDS             1618..2430
                     /locus_tag="P5310_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HRU32901.1"
                     /translation="MKLGVVVSLVEGIEPAIKKVKDLGLSTCQVVSWNTELFTGDNAR
                     ALRESADRWGVEITHLWVGWPGPRVWNFIEGPLTLGLVPPEYRYVRLEALKRGSDFAK
                     LIGVENIVTHVGFIPENPNDPLYPGLISLLKEIVRYCDYNGQYFCFETGQETPTTLLR
                     TIEDIGMKNLGVNLDPANLLMYGKANPVDALDIIGRFVRGVHAKDGEYPTGGRSLGME
                     KPLGEGRVNYPEFIARLKMCGYDGALTIEREISGENQIRDILRAKEFLSRLI"
BASE COUNT          768 a          469 c          549 g          774 t
ORIGIN      
        1 tttggaagta tagtcttaaa acttcctcca ccatttaaaa gccgtctaaa tttgttgtgt
       61 gtatcttctc ccccgtcaag gctcaatact atacttatct ccagttcatc taataggtct
      121 aacgtcttac tgtctaaaag tagtccgttg gttgtgagcg tgaatctcaa gttttttcct
      181 gaatttaacg ccctggtctt tccatatata gtggcatttt tgactacttc cagatttagc
      241 agcggctctc ctccgaaaaa atctatctcg atattcttta tatctttgga agaatctatt
      301 aaaaagtcta tagccctaca ggctatctct agaggcatca gggacatcct gtaagagagg
      361 ttatgagtgg caaagcaata gctgcaagac atattgcagg agtgagacac gttcagacag
      421 agagatttta atcttaaatc ctccggttca tatatctcca gaggtttata tgaggacaat
      481 atctcttcta tctctccata tacatcttct gagatatcaa gagatcctcc tctataaagg
      541 gtatccatag cactcctggc ttcttcatct aactgataca gagaacctaa gctgttgaat
      601 agaaaatgtt ccccccttat agagaataga tgccagtctc tcgttaccag gtccatttcc
      661 ctactgattt ctttacctca cataccagat ttccaaccgt catactagtt ttacatgcag
      721 acctgcaagg ggtttgacac tcctgacatt tctctatctc ttctttcttt gctattggcg
      781 aatctaatat taccctaatg tgttttttca tacatccctc cagtatcgta tcttttaatg
      841 tatctattat aacataactc caaactgtca agcttgccta tacagttctc ttctgatata
      901 ctactaggtg tagaaagacc acttaaggag ggagcttcta tggaggataa tacttattta
      961 gatttgatta agagtcttac tctggagatg ctatcagtat ctaaaaagaa tatcaaagac
     1021 ggaattgact ctctggtaaa taaggatgaa atccttgccg atagagttgt ggagggggat
     1081 aaatatgtag atgagcttga attgaagata gaggatatgt gtataaagtc tctcagaaac
     1141 cttgataagg aagaggatat tagatttatc agtggtatat ggaagattat aacagatgca
     1201 gaacgtatcg gagattatgc aacccatgtg gcagattctg ctaaagtcct tgccagtaaa
     1261 cccaccctta agccccttat cgatataccg gctatggcag aaaatgtagt cgatatgata
     1321 tctagatgcg agtcagctct ggaaagctac gatctttcgt atatagattc tatctgggag
     1381 cttgacaggg aaatcgatgc tctctatgac caggtcttca gggaactttt aagctatatc
     1441 ctggaaaccc caagacttat taccaatgtg atctatctca ctcaggttgc cagatactta
     1501 gaaagagcag gtgaccatgt tacaaatata ggggagagga tattttacat agtaactggt
     1561 aagaggatta agaggggaaa tattcaaagt gaatcataaa acaggaggga tgaagttatg
     1621 aagctaggag tagtagtatc tctggtagaa ggtatagagc cagcaataaa gaaagtaaaa
     1681 gatttaggtt tatccacctg tcaggttgta agctggaaca ctgaactctt tacaggagat
     1741 aatgcccggg cacttagaga atctgcagat aggtggggag ttgagattac tcatctctgg
     1801 gtaggatggc ctggaccccg tgtatggaac tttatagaag gtccgctgac tcttgggtta
     1861 gttccccctg aatatcgtta tgtcaggcta gaggcattga agagaggttc tgattttgcc
     1921 aaattgatag gagtagaaaa tatagtaaca cacgttggtt ttattccaga gaatccaaat
     1981 gaccccttgt atcctggtct tatctctctg cttaaagaga tagtgagata ttgtgattat
     2041 aatggacagt atttctgttt tgagacggga caggagactc caaccacgct tctcaggact
     2101 atagaagata taggtatgaa aaacctgggt gtaaacctgg acccggctaa tctattaatg
     2161 tatggtaagg ctaatcctgt agatgccctg gatataattg gtagatttgt aagaggagtc
     2221 catgctaaag acggggaata cccaacagga ggaaggtccc ttggtatgga gaaaccatta
     2281 ggggagggaa gagttaacta tcctgaattt atagccaggt taaagatgtg tggctacgat
     2341 ggcgccttaa ctatagagag ggaaatctca ggagagaacc agataagaga tatcttgagg
     2401 gcgaaagaat ttctcagtag attgatttga gatgagcaga agaaagatta tagcgttttt
     2461 tattgccctt tcagtcatct cttttatagt attccttctg ggaatttcta tcggaaccgt
     2521 ttattttcca ttttcagaag taataaagag tatcatcttt
//
LOCUS       DAOTKU010000225         2558 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_32877_length_2558_cov_2.990811, whole genome shotgun sequence.
ACCESSION   DAOTKU010000225 DAOTKU010000000
VERSION     DAOTKU010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32877_length_2558_cov_2.990811"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="P5310_05770"
     CDS             complement(<1..250)
                     /locus_tag="P5310_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRU32902.1"
                     /translation="MIRTENLCKKYEKVEAVKDLSISVEPGEIYGFLGPNGAGKTTTI
                     MMILGILKPTSGKILVFNKDLYSDYFGIKRRVGVVSEVQ"
     gene            complement(319..987)
                     /locus_tag="P5310_05775"
     CDS             complement(319..987)
                     /locus_tag="P5310_05775"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HRU32903.1"
                     /translation="MNDVDIRAQLVKWGKRLVDNGLVVAAGGNISARVGDTMFISPSG
                     YSIADATEEDYVPVEISTGKILDDFPLKPSSEVLMHLRCYRVRSDIRAIVHVHPPILV
                     GIISAGGMIQPFTPDSVALLGKPAILDYILPTTDVLARAVEEKVKEADVILLRNHGVV
                     TVGTNLREACYKAEVAEDTAKSFLASRIIGTLKLFTESELEAINGLSSEQYRLEIMRK
                     MQEA"
     gene            complement(1005..2042)
                     /locus_tag="P5310_05780"
     CDS             complement(1005..2042)
                     /locus_tag="P5310_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007931523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent dehydrogenase"
                     /protein_id="HRU32904.1"
                     /translation="MKAQVFYGPQDMRLEDVPIPSPKEGEMLIKVEACAVCGTDVRIY
                     YSGHHNVKPPQVIGHEIVGTIAEIGSGIEGYDVGEKITVVTPVGCGHCKYCISGKVNL
                     CSSFRAIGYNFPGGFAEYMILPQEAVRQGNAIKLPSNANLEGSALIEPLSCVLNGQEY
                     LNIGFTDTVVVIGSGPIGCMHTAVARAKGASKIILADIQAKRLEMAKPFGADIFVDSS
                     KEDLVDLVRKETGGIGADVVITAAPSGQAQEQALQMVAKRGRISFFGGLPGDRATITL
                     NSNIVHYNEVSIFGAYASNRYNYYEAAVLLSSGRVNLEKLITHTLPLEDLVKGIELVR
                     QGEALKVIIKP"
     gene            complement(2051..>2558)
                     /locus_tag="P5310_05785"
     CDS             complement(2051..>2558)
                     /locus_tag="P5310_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32905.1"
                     /translation="EYWEDIYKNIEKFKDEGLLFAEDKILPSEIDKSINLIMDERYRR
                     ILERLLSNIGVGLDNCRIIIDDYRIGKGLEQFLHKLEDNGAEVIAVSRSEDRYLETRV
                     ASVIAKWIRSEELREIGILGTGNTGDKRILEWLEEWYSSYKSWPWFVKRTFRTIREIE
                     NSINIKGR"
BASE COUNT          684 a          665 c          380 g          829 t
ORIGIN      
        1 attgaacctc tgagactacc cctactcttc tctttattcc aaaataatca ctgtacaggt
       61 ccttgttgaa caccagaatc ttaccactgg tcggttttaa aataccaagg atcatcataa
      121 tagtggtagt cttccctgcc ccattcggac ccaaaaagcc atatatttct cctggttcta
      181 ccgatatact gaggtctttt accgcttcaa ctttttcata ctttttgcag agattctctg
      241 tcctgatcat ccattccctc cttccctcta aatgagttaa atatactata ataagaatga
      301 attttcaaca gcaaaagatt atgcctcctg catctttctc attatttcaa gcctgtactg
      361 ctcagagctt aagccattta tagcttccaa ctcagactcc gtaaatagct tcagagtccc
      421 tattatccta ctggccagaa aagacttggc agtatcttca gcaacctctg ccttataaca
      481 cgcctctctc aagtttgtac caactgtaac caccccatga tttctgagca gtatcacatc
      541 agcctcctta actttctctt caacagctct ggccaggacg tctgtagttg ggagtatata
      601 gtcaagaata gcaggtttac ctaacagggc aaccgaatca ggtgtaaatg gctgtatcat
      661 tcctcctgca cttataattc ctacaaggat aggagggtgg acatgaacta ttgcccttat
      721 atctgacctt accctgtagc atctgagatg cataagcacc tctgatgatg gcttcagagg
      781 aaaatcgtcc agaatttttc cggtgcttat ctctacagga acataatctt cttctgtagc
      841 atcggctata gaatatccac ttggagagat aaacatggta tcacctactc tagcgctgat
      901 attccctcca gctgccacaa ccagtccatt atctaccagt cttttccccc actttactaa
      961 ctgagctctt atatctacat cattcatttg atctttctcc tttcctatgg ttttattatt
     1021 acttttaacg cttctccctg tcttacaagt tcaataccct ttaccaaatc ctctaagggc
     1081 aaggtatgag ttataagctt ttccagatta accctgccac tggataatag taccgctgct
     1141 tcatagtaat tatatcgatt agatgcatat gcaccaaaga tagagacctc attatagtga
     1201 actatattac tgttcaacgt tatggtagca cgatctccgg gcaagccacc aaagaaactt
     1261 attctacccc ttttagctac catctgcagg gcttgctctt gagcctgacc tgaaggtgct
     1321 gccgttatta ctacatctgc tccgatacca ccggtctctt tccttaccag atctaccagg
     1381 tcctccttag aagaatcgac aaatatatca gcaccaaagg gtttagccat ctccaatcgt
     1441 tttgcctgaa tatctgccag gattatctta cttgcacctt tagctcttgc cacagcagta
     1501 tgcatacaac caataggacc agacccgatt acaaccacag tatcggtaaa acctatattg
     1561 agatattcct gtccatttaa cacacatgag agtggttcta taagagcaga tccttctaaa
     1621 tttgcattgg atggcagttt tatagcattt ccctgtctta ccgcctcctg gggtagtatc
     1681 atatactctg caaatccacc cgggaaatta tatcctatag ccctgaagct ggaacataga
     1741 tttactttac cggagataca atacttgcaa tgtccgcatc caacaggggt taccactgtg
     1801 attttttctc caacatcgta accttctata ccactgccaa tctctgcaat agtacctacg
     1861 atctcatgcc cgattacctg tggtggtttt acattatgat gcccggaata atagattctt
     1921 acatctgtac cacataccgc gcatgcttct accttaataa gcatctcacc ctctttgggt
     1981 gatgggattg gaacatcttc taatctcata tcttgaggtc cataaaaaac ctgcgccttc
     2041 attacactct tcacctgcct ttaatattta tagaattttc tatctctctt atcgttctga
     2101 aagtcctctt tacaaaccat ggccagctct tataagagct ataccattct tccagccact
     2161 ctaatatcct tttatctcct gtattcccgg ttccaaggat accaatctct ctgagctctt
     2221 cagatcttat ccacttggca ataactgatg ccaccctggt ttcaaggtat ctatcctcag
     2281 acctgctaac agctattact tctgccccgt tatcctccaa tttatggagg aattgctcta
     2341 atcctttacc tatcctgtaa tcatctatta ttattctgca attatccagt cctacaccta
     2401 tattagacag taatctctcg agaattcttc tgtatctctc atccataata aggtttatac
     2461 tcttatctat ttcagaaggc agtatcttat cttctgcaaa taataatcct tcatccttaa
     2521 atttctctat atttttatat atatcttccc agtactct
//
LOCUS       DAOTKU010000226         2553 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_32965_length_2553_cov_3.086069, whole genome shotgun sequence.
ACCESSION   DAOTKU010000226 DAOTKU010000000
VERSION     DAOTKU010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32965_length_2553_cov_3.086069"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            274..1275
                     /locus_tag="P5310_05790"
     CDS             274..1275
                     /locus_tag="P5310_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32906.1"
                     /translation="MINFLDLKTPSFIKLFDPKDSIPVASPPEKGPGYWAGATSVLYD
                     KEIGKFYLYYRLRRPRLVENGKEIGRGYECFIAESTDGIDFKTLWKVDKSSFGGISLE
                     KASLIKTLDGIYRLYISYVDPEDNRWRIDMLEAKRIEDLNPVEKVKIFTPSELQIEGV
                     KDPEVIIIGGLYYMFTSYAPSPEKITPELKVKMHESADIYNTGITKSCTGLAISSDGI
                     NFRWIGDILSPSLKGWDAYATRITSILYSPPIFYAFYDGSRDVSENYEEKLGLAISLD
                     LMRFERVTAEGPLIVSPYSSGSLRYLNVTPVDGKLYYYYEISTEDGSHELRVNIVES"
     gene            1265..>2318
                     /locus_tag="P5310_05795"
     CDS             1265..>2318
                     /locus_tag="P5310_05795"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HRU32907.1"
                     /translation="MNLRGVNHQLVKIKNRNLILRLIYERGSISRQEISRLIGLTPAT
                     VTNITGELLEKGFITELSNFREKSSAGRKAVPLAINPTAGYVIGVDIRPKIVRMSISD
                     LLGRLSNIESFPYNSNRDELIETLVEKLQVLTEDRDILGIGIGVPGLVDSSSGEVKIS
                     PNLGWKNLNLSGPIEKSLRIPVTVENNVRVMALGERWFGKGKKSDNFILVYVSLGVGS
                     GIVISGKIYKGINNSAGEIGHTVVSEDGELCNCGKKGCLETFASARAIVKNFKGTSLD
                     EDSDIEEIKSAIEKGDKRAIEIVNRAGHYLGVGISNLVNILDPEMIILAGRVIRLGKT
                     LLFPMRESLESRLFSTN"
     assembly_gap    2319..2328
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          870 a          394 c          597 g          682 t
ORIGIN      
        1 atgataataa tcccatgctt ttcttctcct cccacatctc ggggaattaa cctctatccc
       61 ccccaatctc aggggaggat agaggtgggg gctgggaggg gggaatagga tgatttccaa
      121 gcaaggagag aaagaaataa aatcaatcat attttttacc gattacgcat cacgcgtcac
      181 gtgttacgcc tcactgcttt taacagaaat cttgacagga atcttttgtg tcgtataata
      241 attaatacag tgaattaatt aaaaggagaa gagatgataa attttttaga cttaaaaaca
      301 cccagcttta taaaactctt tgatccgaaa gatagcattc ctgttgccag cccaccagaa
      361 aaaggtccag gatattgggc aggagctacc agtgttttat acgataagga gataggaaag
      421 ttctatctat attacaggct gaggagacca agactggtag aaaatgggaa agagattggg
      481 agaggctacg aatgttttat agctgaaagc accgatggga tagactttaa aactttatgg
      541 aaggtggata agtcctcctt tggaggtata tcactggaaa aggcttctct cattaagaca
      601 ttagatggga tatatagact ctatataagc tatgtagatc cagaggataa caggtggcga
      661 atagatatgt tagaagctaa aaggatagaa gaccttaatc cagtagagaa ggttaagata
      721 tttacccctt ctgagctcca gatagaagga gtaaaagatc cggaagttat tatcatcggt
      781 ggtctttact atatgtttac aagctatgcg ccatccccag aaaaaataac accagagtta
      841 aaggtgaaga tgcatgaatc agctgatata tataatactg gaatcactaa atcctgcaca
      901 ggattagcaa taagttctga tgggataaac ttcaggtgga taggcgatat actatcccct
      961 tctctaaaag ggtgggatgc ctatgcaacg agaataacaa gtattctcta ttctcctcca
     1021 atattctacg ccttctatga cggaagcagg gatgtatctg aaaattatga ggaaaaactt
     1081 ggattagcta taagcctcga tctcatgaga tttgaaagag taactgctga aggtccatta
     1141 atagtttctc catattcttc tgggagtcta agatatctta atgtgactcc tgtagatgga
     1201 aaactttatt actactatga aatatctaca gaagatggtt ctcacgaatt gagggtgaat
     1261 atagttgaat cttagagggg taaatcatca gctggtaaaa ataaagaaca ggaatctcat
     1321 ccttagactt atatatgaaa gaggctcgat aagcagacag gagatatcca gattaatagg
     1381 gctaacccca gccacagtaa caaatattac tggagaactt ctggagaagg gatttataac
     1441 agagttgtca aacttcagag aaaagtccag tgctggcaga aaagcagttc ctcttgccat
     1501 aaatccaact gctggttatg tgataggtgt agacataaga ccaaagatag taaggatgtc
     1561 catcagtgac cttttaggac gtctatccaa tatagagagc ttcccataca atagtaacag
     1621 agatgagtta atagagacac ttgtagaaaa attacaggtt ctaacagagg atagagatat
     1681 cttagggata ggtataggag ttccagggct tgtagattca tcttcaggag aagtaaagat
     1741 ctcaccgaat ctgggttgga agaatctaaa cctttcaggt ccgatagaga aaagcctgag
     1801 aattcctgta acagtagaaa ataacgttag agtgatggca ttgggagaga ggtggtttgg
     1861 aaagggcaaa aaatcagaca attttatctt agtttatgtg agtttagggg taggctctgg
     1921 gattgtaata tctgggaaga tatataaagg aattaataat agtgctggag agatagggca
     1981 tactgtagtc tctgaagatg gagaactctg taactgtggg aaaaaaggat gtctggagac
     2041 ttttgcttct gccagggcga tagtaaaaaa ctttaaagga acatctttgg atgaagattc
     2101 agatattgag gagataaaat ctgctataga aaagggcgat aaaagagcta ttgagatagt
     2161 taaccgtgct ggacactact taggggttgg tatatcaaac cttgtaaaca tccttgatcc
     2221 tgagatgata atactggctg gtagggtaat tagactgggt aagacccttc tttttcctat
     2281 gagagaatct ctagagagca gactcttctc aactaatann nnnnnnnnaa ggatagaaat
     2341 ttcagagctg ggtaaagaga ttggactata cggggctagt gccttagccc ttgaaaaatt
     2401 tttctatgat aaggaggaat tatgaatata ggtatagtag gagcaggtag gattgctaaa
     2461 gcccattttg agaggctgag taaattgaaa gatatcaaga taaaaggggt agcagacata
     2521 atattcgtaa gagccaaaga gatggctgaa aag
//
LOCUS       DAOTKU010000227         2548 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_33050_length_2548_cov_2.995187, whole genome shotgun sequence.
ACCESSION   DAOTKU010000227 DAOTKU010000000
VERSION     DAOTKU010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33050_length_2548_cov_2.995187"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(238..313)
                     /locus_tag="P5310_05800"
     tRNA            complement(238..313)
                     /locus_tag="P5310_05800"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(277..279),aa:Glu,seq:ctc)
     gene            361..1281
                     /locus_tag="P5310_05805"
     CDS             361..1281
                     /locus_tag="P5310_05805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HRU32908.1"
                     /translation="MSRDMIERKSLWDIDYIIVDFETTGSLPSLDSIIEVGAVIFSNG
                     RIVDSFSSFVYPERNIPPFITFLTGITHEMLRSAPQASDVIPKLMKLIDGKVFVAHNA
                     KFDLSFLNSQLEKLGMPQYAGDVLCTLHLARRLLKLPRRGLDSLAEYFGIDIPHRHRA
                     LYDAEATALILERLFKLLNNEGIFTWKELQKKLDGEYDIPKNFLKLKPILEYVPHAPG
                     IIIFLDKLGDVLYVTKSKDIKNKISNYFYIQDNAPKNRKDLLISTYSLEYITTNTEFE
                     AYLLEKELLQILEPRFNRIYKKGTSSQTSS"
     gene            complement(1286..1501)
                     /locus_tag="P5310_05810"
     CDS             complement(1286..1501)
                     /locus_tag="P5310_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HRU32909.1"
                     /translation="MSMLGKILILFGIVFILLGIFLIFFQRIPYLGKLPGDIIIRRGN
                     TVIYFPIVTCILLSIIFSLILNIFFRR"
     gene            complement(1507..2058)
                     /locus_tag="P5310_05815"
     CDS             complement(1507..2058)
                     /locus_tag="P5310_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009064234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HRU32910.1"
                     /translation="MKLLLHICCARCAIYPTERLKSEYEIIGYYFNPNIQPATEYLLR
                     GEALIGYARNKEIELIVGEYDPYTHIRSVVNRLDDRCDVCYKLRLTEAARKAKELGCS
                     YFSTTLLSSPYQNHTRIKEIGEYISREIGDIEFYYEDFRGKWREVQKMADEMGIYKQK
                     YCGCIFSEYERFQKKTKRLTSLR"
     gene            complement(2055..>2548)
                     /locus_tag="P5310_05820"
     CDS             complement(2055..>2548)
                     /locus_tag="P5310_05820"
                     /inference="COORDINATES: protein motif:HMM:NF037140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Holliday junction DNA helicase RuvB C-terminal
                     domain-containing protein"
                     /protein_id="HRU32911.1"
                     /translation="LRDRFGIVERLDYYSPEEILKIIERAAKILNIKYEKEALREISF
                     RARGTPRIANRIIKRIRDYSDVKGNGFISRSLALEALALFEIEEHGLDREDIRILETI
                     AYKFGGGPVGIETLSSAIGESAETIEEIYEPYLIKMGYIERTPRGRTITENGLDILKG
                     KER"
BASE COUNT          816 a          519 c          414 g          799 t
ORIGIN      
        1 acgagacttt cgtagcttac tatgaggttt ttccaggaca gacaactaat attaacatat
       61 cactaaaccc tataagatag tcagaataac taggtgagta atcgcaaggt gtgatgatgc
      121 caccttacaa agtagagccc ctgttaacag gggctctact aaatagagct atacaataat
      181 ttaatcttca acttttgcat gggaaaaaca agaataactg ggaaataata aaaaatctgg
      241 tagcccctag gggagtcgaa cccctgtctt cgagctgaga acccgacgtc cttggccact
      301 agacgaaggg gccagtcaat tgactaaatg cagtaattat attacaatat attagactat
      361 atgtcaagag atatgataga aagaaagtct ttgtgggata tagattatat tatagttgat
      421 tttgagacta ccggtagcct accgtcactt gacagcataa tagaggtagg tgctgttata
      481 ttcagtaacg gtagaatagt tgattcgttc tccagttttg tctatcctga aagaaacatc
      541 ccacctttta taacattttt aaccggaatc actcacgaaa tgttaagatc tgctccccaa
      601 gcttcagatg taattccaaa gttaatgaaa ttgatagatg gcaaggtatt tgttgcccat
      661 aacgcaaaat tcgatctcag ttttttaaac tctcaactgg aaaaactagg aatgcctcaa
      721 tatgctgggg atgttctttg cacattgcat cttgcacgtc gcttacttaa attaccaaga
      781 agagggttag atagcctcgc tgaatatttt ggtatagata tacctcacag acacagggct
      841 ttatatgatg cagaggcaac ggcattgatc ctagagagac tatttaaatt actcaataat
      901 gaaggtatct ttacctggaa agaattgcaa aaaaaacttg atggagaata tgatatcccc
      961 aaaaacttcc tgaaacttaa accgattctt gagtatgttc ctcacgcacc gggaatcatc
     1021 atattcctcg ataagctggg tgatgtgtta tatgttacaa agtcgaaaga tataaagaat
     1081 aagatatcaa attactttta tattcaggac aatgctccaa aaaatagaaa agacctcctt
     1141 atcagtactt attctctgga atatataaca acaaatacag aatttgaggc ttatctacta
     1201 gaaaaagagc tattacagat attagaacca agattcaaca gaatatacaa aaaggggaca
     1261 tccagtcaga cgtcttcctg atctgttatc ttctaaaaaa gatattaagt attaatgaga
     1321 agataatgct cagcaatata caggtaacta tgggaaagta aattaccgta tttcctcttc
     1381 ttattattat gtctccagga agtttaccga ggtatggtat acgctgaaaa aatattagaa
     1441 aaataccaag taaaataaat actatcccaa aaagaattaa tatctttccc agcatactca
     1501 tctgcatcac ctcaaagagg taagtctttt agtcttcttc tgaaatctct catactcact
     1561 gaatatacat ccacaatact tctgcttata gatacccatc tcgtctgcca ttttctgtac
     1621 ttctcgccat tttcctctaa aatcttcata gtagaactcg atatctccta tttctctgga
     1681 tatatattct ccaatctcct ttattcttgt atgattctga tatggactgg ataaaagagt
     1741 ggtggaaaaa taagaacaac ccaactcttt agcctttctg gcagcttcag ttaatctaag
     1801 tttataacag acatcacatc tatcatcaag acgattaacc accgatctta tatgagtata
     1861 agggtcatat tctcctacta taagttctat ctctttattt cttgcatatc ctatgagggc
     1921 ttctcccctc aacaggtact ccgtcgcagg ctggatatta gggttaaaat aatatcctat
     1981 aatttcatac tcagacttta atctttctgt cggatatatt gcgcacctgg cacaacatat
     2041 atgaaggagt aacttcatct ttcttttccc ttaagtatat ccagaccatt ctctgtaata
     2101 gttctacccc taggtgtcct ctcaatatac cccatcttta taagataagg ctcgtatatc
     2161 tcctctatag tctcggcaga ttcccctatg gctgatgata gagtttctat gccaaccggt
     2221 cctcctccaa acttatacgc gatagtttcc aggatcctta tatcttctct atcaagtccg
     2281 tgttcttcta tctcaaaaag tgcaagggct tccagagcta aagaacgtga gataaaacca
     2341 ttacccttta catccgaata atctcttatt ctctttataa tcctgttggc aatacgaggt
     2401 gttccccttg ccctgaaaga gatctctcta agggcttcct tttcatactt tatattgaga
     2461 atcttagctg ctctttcaat tatcttcagg atctcttctg gagaatagta atctaatctt
     2521 tctactattc caaatctatc tcttaaag
//
LOCUS       DAOTKU010000228         2546 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_33073_length_2546_cov_4.187876, whole genome shotgun sequence.
ACCESSION   DAOTKU010000228 DAOTKU010000000
VERSION     DAOTKU010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33073_length_2546_cov_4.187876"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            216..1511
                     /locus_tag="P5310_05825"
     CDS             216..1511
                     /locus_tag="P5310_05825"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRU32912.1"
                     /translation="MKKLLVLILSIVFLMTFLNTGLFAQQQKVIKLNAMGWVQAEWPL
                     KDLAAQYEKLHPNIKIVYEDVGIDLKKYQQLVKEGKFEWNALLGGWPATDTAPGFISG
                     MYQHMDTYVKKSKEKDAPLILKDMLPSVLAESKFRGRTLASLPITIDIDALLYRKDVL
                     KSVGYEKPPVTTWQDINTLAEKIVSAFKPKGIWGISTSVRVFHRGPAAIQQTFTKKPF
                     LPSGLMDVAGDGMIKTMQLLKYWIDKGLSMPDSMWYPFPGDAFAEPYLKGQYGMFVYQ
                     HSAGIWAYQTVGKENCGLTRLPKETAGGTVFWSTPAYLYKDAPYPQETVDFLVWAFGP
                     NNDFTWRKVFESGKLPPYRTPYTKIAKGNPDFEWALDMVNVLEEATPIPNTTNFGAQL
                     DIVRPWAEKYLSGQIKDPLEAMKGAKKELDKQLSKLIFK"
     gene            1580..2449
                     /locus_tag="P5310_05830"
     CDS             1580..2449
                     /locus_tag="P5310_05830"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRU32913.1"
                     /translation="MKSKYYSNFIYILPALSFILIFNIWPIIWGVVLAFMDFRGVTLT
                     GEWVGLGNFKELISDERVWQGLSISAKYVLYSYPATQLLALVSGWMISELRAKTVGKI
                     YRVILYLPVVIPVSAGMTMFKALYESSGYLNIMLTKLGLSPVGWLTDPNVAIYSLAIA
                     SIWRNFGASMFYYLIGFYNVPLEVKESALIDGASLWTRFWKVDLPAIKNMLFIIFIQS
                     AGALTGGLAEVTALTAGGPARATAILPYYSAEISIWGEQRLGYAAAINLFLSIFTLIL
                     ASVVIRLVKVEKE"
BASE COUNT          779 a          429 c          597 g          741 t
ORIGIN      
        1 ccatagctcc catagatagg atagatgtat tgattacaga tagcctggca gacaagagga
       61 ttttaaaaat ccttgaagat aaagggattg aagttctgat tggggaggtg ggagattaga
      121 ataatggtaa gagaagattg aactatatga atagtgatta acagggatga ggaaataaca
      181 gggggaagat ttatgaaaaa atggaggagg gatttatgaa aaaattattg gtacttatct
      241 taagcatcgt gtttttaatg actttcctga atactggact cttcgctcag cagcagaagg
      301 tcattaagct aaatgctatg ggttgggttc aggcggaatg gccacttaaa gatttagctg
      361 ctcaatacga gaaattacat cctaatatta agattgtcta tgaagatgtc ggtatcgacc
      421 tgaagaagta tcaacagctg gtaaaagaag gaaaattcga gtggaacgct ctgcttggtg
      481 gttggccagc aaccgataca gctcccggtt tcatctccgg tatgtaccaa catatggata
      541 cttatgtaaa gaagtctaaa gaaaaagatg cccctcttat cttaaaagat atgcttccat
      601 ctgttttagc tgagagcaag ttcagaggac gtactttagc gagtctacct ataactatag
      661 atatagatgc attactttat agaaaagatg tgcttaaatc agtagggtat gaaaaacctc
      721 cggttacaac ctggcaggat ataaataccc ttgcagaaaa gattgtaagc gcatttaaac
      781 caaaaggtat atggggaatc tctacaagtg taagggtgtt ccatcgaggt ccggcagcta
      841 ttcagcaaac cttcaccaag aaaccattcc tgccgagtgg acttatggat gtcgctggcg
      901 atgggatgat aaagaccatg caattattga agtactggat agataaagga ctttctatgc
      961 ctgatagtat gtggtatccg ttccctggcg atgcatttgc agagccatac ctgaaaggtc
     1021 agtatggcat gtttgtttat cagcattccg cagggatatg ggcatatcag actgtgggta
     1081 aggaaaactg tggacttaca agacttccta aggagacagc aggaggaaca gtattctgga
     1141 gtacacctgc ttatctttat aaagatgcac catatccgca ggaaacggta gattttctcg
     1201 tctgggcatt tggaccaaac aatgatttta cctggaggaa agttttcgaa agtggaaagc
     1261 tccctccata cagaactcca tacacaaaaa tagccaaagg taatccagac tttgaatggg
     1321 ctcttgatat ggttaatgtt ttagaggaag ctacgccaat tcctaacact accaactttg
     1381 gagctcagct agatatagtc agaccatggg ctgaaaaata tctaagtgga cagattaagg
     1441 atcctctcga agccatgaaa ggtgcaaaga aagagctgga taaacagtta tctaaactaa
     1501 tctttaaata agttttcatt cctgggggta gaaaaatttc ttatccccag gaatatctat
     1561 atctggtgga ggattcttta tgaaatctaa atattatagt aattttattt atatcttacc
     1621 tgcattatct tttatcctta tattcaatat atggccaatt atatgggggg tagtattagc
     1681 ttttatggac tttagagggg tcactcttac aggggaatgg gttggcctgg gtaattttaa
     1741 agagttgatc tctgatgaga gagtgtggca gggtctatct atctctgcca aatatgtatt
     1801 atatagttat ccagccacac agctgctggc tttagtcagt ggatggatga tatcagagtt
     1861 gcgagccaag accgttggga aaatatacag agtgattctg tatttaccgg tagtaatacc
     1921 ggtctcggca gggatgacca tgttcaaggc tctttatgaa agcagtggat atctaaacat
     1981 tatgttaact aaattgggct tatctcctgt tggatggtta accgatccca atgtagctat
     2041 ctactcttta gctattgcaa gtatatggag gaactttgga gccagtatgt tctattatct
     2101 gataggtttt tataatgtcc ctttagaagt aaaggaatcg gcacttatcg atggtgcaag
     2161 tttatggact cgattctgga aggtagacct gcctgcgata aagaatatgc tttttataat
     2221 atttattcag tctgcaggag cactgactgg aggtttagct gaggtcaccg ctctgaccgc
     2281 tgggggacct gccagggcaa ctgcgatact accatactac tctgctgaaa tatctatatg
     2341 gggcgagcag agattaggtt acgcagctgc cataaatctc ttcttatcta tatttactct
     2401 gatactggct tctgtggtaa taaggctggt gaaggtagag aaggaataag aatgaggagg
     2461 acaatatgtt ttctctgaaa agaagggaga ttttcaggca acaggttact tttaatatta
     2521 tcctggcagt tcttgccata gtagta
//
LOCUS       DAOTKU010000229         2545 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_33094_length_2545_cov_3.284739, whole genome shotgun sequence.
ACCESSION   DAOTKU010000229 DAOTKU010000000
VERSION     DAOTKU010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33094_length_2545_cov_3.284739"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /gene="rlmB"
                     /locus_tag="P5310_05835"
     CDS             <1..534
                     /gene="rlmB"
                     /locus_tag="P5310_05835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HRU32914.1"
                     /translation="IPSLDAKQGILAYIFPVQPRDIEDFLDKEFVLLADGIEDPHNLG
                     AIIRTAECAGVDGIVIPKHRSALISQGLINSSAGAIFHLPFAVVNNTAQAVDKFLNAG
                     FSVLGADPEGETIFEVDFKFPVLLIIGGEDTGLRPIMKKRCDRLIGIPMRGKITSLNT
                     SVSAGIIIYEIVRRLWR"
     gene            539..1378
                     /locus_tag="P5310_05840"
     CDS             539..1378
                     /locus_tag="P5310_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019908012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HRU32915.1"
                     /translation="MKKGINIWSFPQGMSIEECMRVAKSAGFEGIELSINETGELGLE
                     SNRNDVLKYKKMAEDTGIEISGLASGLYWKYPFTSNSREIREKAKDICKKQIEFANIL
                     GVDTVLVVPGYVGVDFIPGSEVIEYDRAYDNALEAISELSRFAESSNVCIGVENVWNK
                     FLLSPLEMRDFIDKINSKNVGAYLDVGNIIYTGYPEHWVKILAHRIKKVHVKDYRRQG
                     CGLSGFVDLLAGDVDFPKVISALKDIGYNDYLNAEMSAYKHYPLQMIYNTSRSLDKIL
                     GRD"
     gene            1380..2405
                     /locus_tag="P5310_05845"
     CDS             1380..2405
                     /locus_tag="P5310_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020620914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRU32916.1"
                     /translation="MIKTGLIGIGFMGRGHLDNYIRLESEGFPVKLVAVCDIDPNKFE
                     NKFLPGNIDVGNQKYDFSKYRLYTDIEEMLNKEELDYVDICLPTYLHAEATIKALNRG
                     LNVLCEKPMALNSRECSEMIEAAKRNNRKLMIAHCLRFWPAYEYLKECVETKRFGEFL
                     GGYFYRGGSPPRWSYKNWLLTREKSGGCMLDQHVHDVDTINWLFGKPRAVSTIARNVI
                     PGSGYDLLSTNYIYPNGKVINAQDDWTLQGDYGFEMLFRANFEGGNLVFQNNILKVNP
                     NDAKGFIPELSKDIGYYREIKYFVDCLSSNKPVTVAPPESSKETIEIVEAEVRSADKD
                     GEITPVL"
BASE COUNT          843 a          411 c          608 g          683 t
ORIGIN      
        1 atcccttctc tggatgcaaa gcagggtata cttgcatata tatttcctgt ccagcccaga
       61 gatattgaag attttttgga caaagaattt gtcctcctgg ctgatggtat agaagatcct
      121 cataatctgg gggctataat aaggactgct gagtgtgcag gagtagatgg gatagtaatt
      181 cccaaacacc gctctgctct catctcccag gggttgataa attcttcagc aggagcaata
      241 tttcatcttc cctttgcagt tgtcaacaat acagcccaag cggtagataa atttctaaat
      301 gcagggtttt cagttttagg ggcagaccct gaaggagaaa ccatctttga ggtggacttc
      361 aagtttcctg ttctattaat aataggtgga gaagatacag gactgagacc gataatgaag
      421 aagaggtgtg acaggcttat tggtatccct atgaggggta agatcacatc tctcaatact
      481 tcggtatctg ctggtattat tatatatgaa atcgtaagaa ggttatggag gtgaaaaagt
      541 gaaaaagggg attaatatct ggtcatttcc acaggggatg agcatcgagg agtgtatgag
      601 ggtagccaaa tctgccggtt ttgaaggtat agagttatcc ataaatgaga cgggagagtt
      661 gggtctggag agtaatagga atgacgtttt aaagtataaa aagatggctg aagatacagg
      721 tatagagata agtggactgg catctggtct atactggaaa tatccattta ccagtaactc
      781 cagagagatc agggaaaagg caaaagatat ctgtaagaaa cagatagagt ttgccaatat
      841 actgggtgtt gatacagtac tggtggtccc cggatatgtt ggggttgatt ttataccagg
      901 atcagaggtc atagaatatg atagggctta tgataatgca ttagaagcta taagcgaact
      961 ctccagattt gcagaatcta gtaatgtatg tataggggta gaaaatgtgt ggaataaatt
     1021 tctcctttca ccgctagaga tgagagactt tatagacaaa ataaactcta agaatgtcgg
     1081 agcctatctg gacgttggta atatcatcta tacaggctat ccagaacact gggtgaagat
     1141 tctggctcac aggataaaga aggtccatgt aaaggactac aggagacagg gatgtggtct
     1201 atcaggattt gtagaccttt tagctggaga tgtagatttt ccaaaagtaa tctctgcact
     1261 caaagatatt ggatataatg actatctcaa cgcagagatg tcagcataca aacattatcc
     1321 tctccagatg atttataaca catccagatc tttagataaa atacttggga gggattaata
     1381 tgataaaaac tggattaatt ggaataggat ttatgggaag agggcattta gacaactata
     1441 taagacttga atcagaaggt tttcctgtaa aactggtggc ggtatgtgac atagacccga
     1501 ataaattcga aaacaagttt cttccaggaa atatagatgt tggaaatcaa aaatatgatt
     1561 tcagcaaata cagactctat acagatattg aagagatgct gaataaagag gagttagatt
     1621 atgtggatat atgtcttccc acatatcttc atgcagaagc cactataaaa gcactaaata
     1681 gaggattaaa tgtcctatgc gaaaaaccta tggctttaaa ctccagagaa tgcagtgaga
     1741 tgatagaggc ggcaaagaga aacaacagaa aacttatgat agcccattgc ctaaggttct
     1801 ggcctgcata tgagtatctg aaagaatgcg tggagacaaa aagattcgga gaattcttgg
     1861 gtggatactt ctatagagga ggtagtcccc caagatggtc ttataaaaac tggttactta
     1921 ccagggagaa gagtggcggc tgtatgctgg accagcacgt gcatgatgtg gataccataa
     1981 actggttatt tggaaaaccc agggctgttt ctacaatagc cagaaatgta attccaggaa
     2041 gcgggtatga cctcctttct acaaattata tatatccaaa tggcaaggtg ataaacgctc
     2101 aggacgactg gacattacag ggcgattatg ggtttgagat gctcttcagg gcaaattttg
     2161 aaggaggaaa tctggttttt cagaataaca tcctgaaggt taatccgaac gatgcgaaag
     2221 gctttattcc ggaattatcc aaagatatag gatactacag agagataaaa tatttcgtag
     2281 actgtctaag tagcaataaa ccagttacag tagctccacc ggaaagcagt aaggagacca
     2341 tagagatagt agaggcagaa gtcaggtctg ccgacaaaga tggggagata accccagtat
     2401 tatagaatta gagccccggt attagccggg gctctatcaa atcctttcca gaacatcggt
     2461 agcttcttct aaaagtaaat catttatctc ttgtgaaaat ggaaatacat taaagatctt
     2521 gggggcttga taacaggcat aatct
//
LOCUS       DAOTKU010000230         2544 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_33108_length_2544_cov_2.898353, whole genome shotgun sequence.
ACCESSION   DAOTKU010000230 DAOTKU010000000
VERSION     DAOTKU010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33108_length_2544_cov_2.898353"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="P5310_05850"
     CDS             <1..612
                     /locus_tag="P5310_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32917.1"
                     /translation="NSSPTSQLVRLHLPGIKRVGVLFKDAGSDVFTDISLDMLSLVLA
                     PEQMVLLGLGDGTNFELLGECADEPVPVEERPAGIVFHRISRDEFEGLWNPTKDQLLR
                     ITVQTFDRDIALRLKAQKEGMDGKGPAIQDSISVTAYQNDEILPLYNWEPNRAVWSGC
                     SWINVLYNTTSIENSKPVKIKVKLNVDTPVIPEISSWVLNYRL"
     gene            complement(622..2154)
                     /locus_tag="P5310_05855"
     CDS             complement(622..2154)
                     /locus_tag="P5310_05855"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HRU32918.1"
                     /translation="MEEELILTVDVGTGSIRAIVFNSDGEVVAKRSVKSNLYQEHFNW
                     AVQKPEEILDNTVNVCRSVINEIKREKKGNLIGWTISTYYHSFLLVDKDNRPVTEILT
                     WADLRASRQAEEIKKRYNSSIIYQKTGCPVHPMYPLSKLIWFMENYPELKDFRVSSAK
                     DYLIYSLFGDWIIDETIASTLGFLNINSLKWDEDILSLIGIKKDKLSDILSPLTIIDG
                     KRLKIDVEDLKDIPFILGSGDGALSSLGMGAINQGDVGVMVGTSGAVRSFSDKPVLDE
                     KERIWCYYFASGLWLIGGAINNGGIALRWLRDGFFQDLVREADALNVDPYDLITKIAE
                     KIPPGADGLIVLPFLTGERSPFWNDKAQGAAIGFNLNHNRADLARAIMESVCFRLFSV
                     YSALKDTFNVDKFNDVRAGGGFSRSNLWLQIMSDVFGEGLTVPALEENTALGAFILSL
                     VALGKLESFGKAKDMVKIRKMVEPDMNNHSIYSELYDIYLDAYWSLNNIYQRLYSFKE
                     KI"
     gene            complement(2157..>2544)
                     /locus_tag="P5310_05860"
     CDS             complement(2157..>2544)
                     /locus_tag="P5310_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010935934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HRU32919.1"
                     /translation="NIKSASREEFIDITGRIQKIIDNSGIKEGLCVVYIPHTTAGITI
                     NEDADPDVINDIKNYLDRMVPNAEYFKHLEGNSPAHIKSSIIGHSITVPVSEGRLLLG
                     RWQGIFFCEFDGPRSRQIIVKIIRED"
BASE COUNT          675 a          594 c          485 g          790 t
ORIGIN      
        1 aactcatctc caacctctca acttgtgagg cttcatctcc cggggataaa gagagttggt
       61 gtgctgttta aagatgcagg gagtgatgtg tttacagaca tctctcttga tatgttatct
      121 ctggttctgg ctcctgagca gatggtcctt ttaggtctgg gagacggtac caattttgaa
      181 ttgctcggcg agtgtgcgga tgaacctgta cctgtggaag aaagacctgc cgggatagta
      241 tttcatagaa tatccagaga tgagtttgaa ggactgtgga atcccacaaa agaccagctt
      301 ttaaggatta cagtacagac ttttgataga gatatagccc ttagactcaa ggctcaaaag
      361 gaagggatgg acggtaaggg accagcgatt caggattcca tatctgtaac cgcatatcaa
      421 aacgatgaga tacttcctct gtataattgg gaaccgaatc gtgcggtctg gtcagggtgt
      481 tcctggataa acgtactgta taatactacc tcaatagaaa attcgaagcc ggtaaaaata
      541 aaggtaaagc ttaatgtgga tacccctgtt atccctgaga tttcttcctg ggtgcttaat
      601 tacagacttt aagttattct cctatatttt ctccttaaag ctgtatagcc tttgatagat
      661 attatttaaa ctccagtagg catccaggta tatatcgtat agctcactgt agatagagtg
      721 gttattcata tccggctcta ccatctttct gattttgacc atatcctttg ccttaccgaa
      781 agattctaat ttaccaagtg ctaccagact gagtatgaag gctcccaggg cagtattctc
      841 ttctagggct ggaacggtta atccttctcc aaatacatcg gacataatct gaagccagag
      901 gtttgatctt gagaatcctc ctcccgccct gacatcgtta aacttatcca cattgaatgt
      961 atcttttaac gcagaatata cgctgaacag tctgaagcat acactttcca ttatcgctct
     1021 ggcgagatca gccctgttat ggttcagatt aaatcctatg gcagctccct gggctttatc
     1081 gttccagaat gggcttctct caccggtcaa gaatggaagg actatcagtc catcagcccc
     1141 tgggggaatc ttctcagcta tctttgtaat caaatcataa gggtccacat tcagtgcgtc
     1201 tgcctctctt actaggtcct gaaagaatcc atccctgagc catctcagag ctattccccc
     1261 gttatttatc gctccaccta taagccatag accgctggcg aaatagtagc accatatcct
     1321 ctctttttca tcaagtacag gtttatctga aaaacttctc accgctccac ttgttccgac
     1381 cataacaccg acatcaccct gatttatagc ccccatcccc agacttgaaa gggcaccatc
     1441 tccagaacca agtatgaacg ggatgtcttt taaatcctct acatctatct ttaacctttt
     1501 tccgtcgata attgtaagcg gagagaggat atccgataat ttatcctttt ttatcccgat
     1561 caggctgagg atatcttcat cccactttaa cgaatttata tttaaaaatc caagggtgga
     1621 agctatggtc tcatctatta tccagtctcc gaagaggcta tagatcagat aatctttggc
     1681 actggaaacc cgaaaatcct tcagttcagg ataattctcc ataaaccata taagcttgga
     1741 tagaggatac atcgggtgca caggacagcc cgtcttctgg tagataatag aactgttata
     1801 tcttttttta atctcttctg cctgcctgct agccctgagg tctgcccagg taaggatttc
     1861 tgtaacaggt ctattatctt tatctaccag gaggaacgag tgataatacg tagatattgt
     1921 ccatccaata aggttaccct ttttttctct tttaatctcg tttataacag atcgacatac
     1981 attaacagta ttatccagaa tctcttcagg tttctgcacc gcccagttaa agtgttcctg
     2041 gtagaggttg ctcttcaccg agcgctttgc cactacctct ccatctgaat taaaaacgat
     2101 agcccttata cttcctgtac ccacatccac tgtcagtata agctcctctt ccatcgctaa
     2161 tcctccctta ttatcttcac tatgatctgt cttgaccttg gtccatcgaa ctcacagaag
     2221 aatatcccct gccatctacc gaggaggagt ctcccttcgc tgacaggaac ggtaattgaa
     2281 tgaccgataa tactgctttt tatatgtgca ggtgagtttc cttccaggtg tttaaaatat
     2341 tcggcgtttg ggaccatcct gtccagataa ttttttatat catttataac atcagggtcg
     2401 gcatcctcat ttatggttat acctgcagtg gtatggggta tatataccac acatagtcct
     2461 tcctttatcc ctgaattgtc tataattttc tgaatccttc cggttatatc aatgaattcc
     2521 tctctggatg cacttttaat attt
//
LOCUS       DAOTKU010000231         2539 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_33196_length_2539_cov_3.422303, whole genome shotgun sequence.
ACCESSION   DAOTKU010000231 DAOTKU010000000
VERSION     DAOTKU010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33196_length_2539_cov_3.422303"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="P5310_05865"
     CDS             <1..239
                     /locus_tag="P5310_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32920.1"
                     /translation="PLMVNTKQGLDQVLSGAFKYCQEDAEHTVFGFYKLQSVWTSETD
                     PLWLGKKGVKEVLDTMIVKVNQAIEENLKGIVVK"
     gene            309..1214
                     /locus_tag="P5310_05870"
     CDS             309..1214
                     /locus_tag="P5310_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRU32921.1"
                     /translation="MKTRMSPMAKREERYFYLCISPWIAGFLIFTAGPIVASLFFSFC
                     NYDVVTPPDWIGIQNYKRLLSDPLFWQSLKVTSYFSLASVPLGIALSLIIAILLNQNI
                     KGLTWFRTIYYMPSVISGVAVALLWMWIFNPEFGVLNFLLWKLFHIQGPAWLMSEEWV
                     IPALIIMSLWGIGGDIVIYLAGLQGIPTELYEAAEIDGANSFNKFLNITIPMMSPVLL
                     FTLIMGIINSFQVFTQAYVMTRGGPHYASLFYVLYIYQNAFQFFSMGYASALSWILFL
                     IILALTFLIFKSSPLWVYYESTRER"
     gene            1230..2081
                     /locus_tag="P5310_05875"
     CDS             1230..2081
                     /locus_tag="P5310_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRU32922.1"
                     /translation="MYLLRSRRKREVLIKSFVYVVLMVTSIIILIPIFWMLSTALKED
                     SEVYLFPPEWIPQKPQFGNFKRALTFIPFGRYFTNTSVITFATMVGSLLSSSIVAYGF
                     ARLRARGRDILFMILLSTMMIPSQITMIPVFVLFKLLNWTDTFKPLIIPNFFGGAFFI
                     FLLRQFYMTIPIELDDAAKIDGCSYLGIFSRIILPLTKPALATVAIFSFMWSWNDFMG
                     PLIYLNSRDKLTLTLALSRFTGMYGMTAWNLLMAASLVVALPCFVLFFFAQRYFIQGI
                     VVTGLKG"
     gene            2193..>2539
                     /locus_tag="P5310_05880"
     CDS             2193..>2539
                     /locus_tag="P5310_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007614651.1"
                     /note="catalyzes the fromation of N-acetyl-D-glucosamine
                     and N-acetyl-D-glucosamine-6-phosphate from
                     diacetylchitobiose-6-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HRU32923.1"
                     /translation="MTEGIVLAVIGGGSSYTPELVEGILKNAEEFPVRTLRLMDIDET
                     KLNIVGSLVGRMIEKENLPIRLELTEDRRTALKGADFIVTQIRVGGIKARIRDEKIPL
                     EFNVIGQETTGAGG"
BASE COUNT          740 a          454 c          555 g          790 t
ORIGIN      
        1 gtcctcttat ggtaaataca aagcaaggac tagatcaggt attgagtgga gcattcaaat
       61 actgtcagga agatgccgag catactgtct ttggatttta taagttgcag tctgtctgga
      121 catctgagac tgatcctctt tggctaggaa agaagggagt taaagaggtc ctggatacta
      181 tgatagtgaa ggttaaccag gcaatagagg aaaatcttaa gggtatagtt gttaaataat
      241 atcaaccctt ttaagggggc aagtagatcc ttgccccctt aaatagatag gatgatggag
      301 aatagagtat gaagacaaga atgagtccta tggctaaaag agaggaacga tatttttatc
      361 tgtgcatttc cccctggata gcaggatttc taatctttac cgctggaccg atcgttgcct
      421 ctctattttt tagcttctgt aattatgatg tggttactcc accagattgg attgggatac
      481 agaactataa aaggctgctc tctgacccat tattctggca gtcattgaag gttacctctt
      541 acttttctct tgcaagtgtc cctttaggga tagcactctc tctaattata gcaatccttc
      601 tgaatcaaaa tataaaagga ttaacctggt tcagaacaat ttactatatg ccatcagtaa
      661 tctcaggggt agcagtagct cttctgtgga tgtggatatt taatccggag tttggagttc
      721 tcaatttctt actctggaag ttattccata tacagggtcc agcctggctt atgagtgagg
      781 agtgggtaat tcctgcactt atcattatga gtctttgggg aataggagga gacattgtaa
      841 tatatctggc agggcttcaa ggtatcccta ccgaactcta tgaagcagca gagatagatg
      901 gggctaattc cttcaataag tttttaaata taacaatccc aatgatgagt ccggtcttac
      961 tatttacact tataatgggt attatcaact catttcaggt atttactcaa gcctatgtaa
     1021 tgaccagagg gggtccacat tatgcctcat tattctatgt attatacata taccaaaatg
     1081 cattccagtt cttcagtatg ggatatgcat cagccttatc ctggatcctg tttctaataa
     1141 tactagccct gacattcctg atattcaaat cttctcctct ttgggtttat tatgaaagta
     1201 ccagggaaag gtaaggagga catctcgata tgtatctatt aaggagtaga cgaaaaagag
     1261 aagtgctgat aaagagtttt gtatatgtgg ttctcatggt aacaagtatc atcatactga
     1321 ttcctatctt ctggatgctt tctactgctt taaaagagga ctctgaggta tatcttttcc
     1381 ctcctgagtg gataccacaa aaacctcaat ttggtaattt caagagggct cttacattta
     1441 ttccatttgg aagatatttt acaaatacca gtgtgataac atttgctact atggtagggt
     1501 cgcttctctc atcatccatc gtggcttatg gttttgcaag gcttagagct agaggtcgtg
     1561 acatcctctt tatgatactc ctctctacta tgatgatccc ttcacagatc actatgatcc
     1621 ctgtctttgt cctctttaaa ttgctaaatt ggacagatac attcaaacct cttatcatcc
     1681 caaacttctt tggaggagcc ttctttatat ttctattgcg tcaattctat atgaccatcc
     1741 ctatagaact ggatgatgca gccaagatag acggatgcag ttatctgggt atcttctcaa
     1801 ggataatact cccccttacc aaacctgccc tggcaacagt cgccatattc tcttttatgt
     1861 ggagctggaa tgactttatg ggtccattga tctatttaaa tagccgtgac aaattgacct
     1921 taactcttgc cttaagtaga tttaccggga tgtatggaat gactgcatgg aatctgctga
     1981 tggcagcatc tctggtagta gctctaccct gctttgtatt attcttcttt gcccagaggt
     2041 atttcattca ggggattgtg gtaactggac ttaaaggtta agatatcact ggggagtatc
     2101 taaaaaccgc cagttgggag agatactctt tgaaaagggc ggaatataag tagcctgacc
     2161 tcaaattaaa tagtttgagt gataggagca ttatgacaga aggaattgtt ttggcagtaa
     2221 ttggaggtgg aagtagttat acaccagagt tagtagaagg aatattaaag aatgcagaag
     2281 agtttcctgt gcgcacatta agacttatgg atatagatga aacaaaactt aatatagttg
     2341 ggtctctggt gggaaggatg attgaaaagg agaatctacc tataaggctt gagcttacag
     2401 aggatagaag aacagctcta aagggcgcag attttattgt tactcagata agggtgggag
     2461 ggataaaggc aaggataaga gacgaaaaga ttcctctaga atttaacgtg atagggcaag
     2521 aaacaactgg ggctggagg
//
LOCUS       DAOTKU010000232         2539 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_33199_length_2539_cov_2.950081, whole genome shotgun sequence.
ACCESSION   DAOTKU010000232 DAOTKU010000000
VERSION     DAOTKU010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33199_length_2539_cov_2.950081"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="P5310_05885"
     CDS             <1..266
                     /locus_tag="P5310_05885"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HRU32924.1"
                     /translation="GEKLLEEMVDYDYNHPSVIIRTIINENWGLDLVNSKEDREWLKR
                     TYEWLKKRDKTRLVVDNSACFPNFHVKTDIEDFHNYFGFPDKF"
     gene            270..1532
                     /locus_tag="P5310_05890"
     CDS             270..1532
                     /locus_tag="P5310_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32925.1"
                     /translation="MRNWVNDLSSHPAWTFGPDMERKGDEPVVVSEFGNWGLPNITKL
                     RAQYGGDPWWFQQRDSRTATKPLGVEERFWGYGLDKVFDNIENLTEAFQELQHQALRF
                     QIEEIRRHNNIKGYIITEFTDLYWECNGLLDITRGKKSYFNELKNINSLDLIFPKERP
                     TGVWGGEGVRIPILFSHLSGSPLENVKISWEMEGTEIKGSFTLKSINYGLGEIGNIEF
                     VVPKVLEPKLFRLNLKATTRNRTISENIINIFVVPKDLVKDRFSLLDGSGAETTSTIY
                     TTAFSDELESLAKQGKTVILELSEETEFSHFGYTLEKRKGITEGRWVSGLGIFSPKLA
                     GRVFRERIMDYRFMDDLPAYYLDGEFKFGSKTLAGMIIGWLYYPMNFMVEVPLGKGRI
                     IMHTFPVINRLSSSPVMSALLYQILERR"
     gene            1645..>2539
                     /locus_tag="P5310_05895"
     CDS             1645..>2539
                     /locus_tag="P5310_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009253518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRU32926.1"
                     /translation="MERPNLIYVFADQLRYDACGYTGNAKAKTPNIDALSREGVSLIN
                     AVSGHPVCAPYRASLFTGKYTTSTGMVINEIRMNPNHVCFAHVLTQSGYKTAYIGKWH
                     LWANELGNHYDPKNSYVPPGPYRLGFDGFWATYNFHHEYYKGYYHTDSLVKIPIEGYE
                     PDGQTDMAINLIREYALSQSPFALFLSYGTPHDPWSLDNVPEEYYKMFKDVKFTLPPN
                     YKEEDDPYADQWGRLSPWERKELTEWMKVYYAMVANLDWNIGRLLKTIDESGLRDNTI
                     FIFTSDHGEMFGSHGRRAKNIF"
BASE COUNT          842 a          358 c          587 g          752 t
ORIGIN      
        1 gaggagaaaa gctattagaa gagatggttg attatgacta taaccatcca tctgtaatta
       61 taagaactat tattaatgag aactggggat tagatttagt aaatagtaaa gaagatagag
      121 aatggcttaa gagaacttat gagtggttaa aaaagaggga taagacaaga ttagttgttg
      181 ataattctgc ctgtttccct aattttcacg ttaaaacaga cattgaagac ttccataact
      241 attttggttt cccggataag ttttaagaga tgagaaactg ggtaaatgac ctttctagcc
      301 atcccgcctg gacatttggt cctgatatgg aaagaaaagg ggatgagcct gtagttgtct
      361 cagaatttgg taactgggga cttccaaata tcacaaaatt aagagctcag tatggaggag
      421 acccctggtg gttccagcag agagattcca gaacggcaac gaagcctctt ggggtagagg
      481 agaggttctg ggggtatggt ctggataagg tcttcgataa tatagaaaat ttaactgaag
      541 catttcagga acttcaacat caggcattga gatttcagat agaagagata agaagacata
      601 ataatattaa gggttatatt attacagaat ttaccgacct ctactgggag tgtaacgggc
      661 tattagatat aacaagaggt aagaagagtt actttaatga gctaaagaat ataaatagcc
      721 tagatttaat ctttcccaaa gaaagaccga ccggagtatg gggtggagaa ggagtaagga
      781 ttcccatact gttctctcat ctctctggta gtcctctaga gaatgttaag atatcctggg
      841 aaatggaagg gacagagata aagggaagtt ttaccttaaa gagtatcaat tacgggttag
      901 gtgagatagg caatatagaa ttcgtagttc ctaaagtctt agagcctaag ctttttaggc
      961 ttaatttaaa ggctaccaca agaaatagaa cgataagcga gaatataatc aacatatttg
     1021 tagttcctaa agatttagta aaagatagat ttagtctttt agatggaagc ggtgcagaaa
     1081 ctaccagtac tatttatacc acagctttta gcgatgaatt agagagtctt gccaaacagg
     1141 gtaagacagt tatacttgag ctttcagaag agactgagtt ctctcatttc ggatataccc
     1201 tggaaaaaag aaaaggtatt actgaaggta gatgggtgag tggacttggt atctttagcc
     1261 caaagcttgc tggaagggtg ttcagagaga ggataatgga ttatagattt atggatgatc
     1321 ttcctgcata ttatcttgat ggagaattta agtttggatc aaagactctg gcaggtatga
     1381 taatcggatg gttatactat cctatgaact ttatggttga agttccgtta gggaagggaa
     1441 gaatcattat gcatactttt cctgttataa ataggctatc cagctcacca gttatgagcg
     1501 ccctgctgta tcagattctt gagaggaggt gagaggggaa aggaaataca tagaagataa
     1561 aaatttttag atacgatctt aggggaagag aagaggttag ttttaaaagc gtgatacctg
     1621 aggatattga taggaggtta aattatggaa agaccaaatc ttatctatgt ttttgctgat
     1681 cagctaagat atgatgcttg cggttataca ggtaatgcta aggctaaaac tcctaatata
     1741 gatgctttat cgagggaagg agtgagtctt ataaacgcag tatcaggtca tccagtatgt
     1801 gctccttata gagcaagttt atttactggt aaatatacaa ctagtactgg gatggttata
     1861 aatgaaatta gaatgaatcc taatcatgtg tgttttgccc atgtcttgac acaaagtgga
     1921 tataaaactg cctatattgg gaaatggcat ctctgggcga atgagctggg taatcactat
     1981 gatccaaaga attcttatgt tccaccagga ccttatagac tgggttttga tgggttctgg
     2041 gcaacttata atttccatca cgagtattat aaaggatatt accatactga tagtctagtc
     2101 aagataccaa tagaaggtta tgaaccagat ggacaaactg atatggctat aaatcttatc
     2161 agggagtatg ctttatctca gagtcctttt gctcttttcc tttcatatgg cactcctcat
     2221 gatccatgga gtttagataa tgtccctgaa gaatattata aaatgtttaa agatgtaaaa
     2281 tttactttgc ctccaaatta taaagaagaa gatgacccct atgcagatca gtggggtaga
     2341 ctttctcctt gggagagaaa agaattgact gaatggatga aggtttacta tgctatggtg
     2401 gcaaatttag attggaatat aggtagatta ttgaagacta ttgacgaatc aggattaagg
     2461 gataatacta tttttatctt tacctcagac catggagaga tgtttggatc tcatggtagg
     2521 agagcaaaaa atatctttt
//
LOCUS       DAOTKU010000233         2534 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_33289_length_2534_cov_3.127471, whole genome shotgun sequence.
ACCESSION   DAOTKU010000233 DAOTKU010000000
VERSION     DAOTKU010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33289_length_2534_cov_3.127471"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /locus_tag="P5310_05900"
     CDS             <1..704
                     /locus_tag="P5310_05900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HRU32927.1"
                     /translation="DLIIVNGENSAGGFGITRGVAEELFSLSVEVITSGNHIWDKKEG
                     MELIIEEERILRPLNYPPEVPGRGYTILNLPNKEKIGIVNLMGRVFTGVSLDCPFRKG
                     MEVVEELHNDGVKSILIDFHAEATSEKIALWRYLDGMVSAVLGTHTHVQTADEEISLL
                     GTAYISDVGMVGSADGIIGEDKDIIIERFLTSLPRRFKVAKGRTLFNGVLLEIDTSSG
                     KAISIERIRKYYKED"
     gene            707..970
                     /locus_tag="P5310_05905"
     CDS             707..970
                     /locus_tag="P5310_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HRU32928.1"
                     /translation="MTTLKVSAKSNPNSVAGALAGVIREEGTVEMQAIGAGAVNQAVK
                     AIAIARGFLAPSGLEIICIPAFVNVEIDNEERTAIKFIVEPRK"
     gene            967..1746
                     /gene="surE"
                     /locus_tag="P5310_05910"
     CDS             967..1746
                     /gene="surE"
                     /locus_tag="P5310_05910"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF001492.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HRU32929.1"
                     /translation="MTISSPTILVSNDDGLGTTGLSVLGRCLSRLGRVVVVSPDRQRS
                     AGGHSITLREPIRMKRLDLEIETWVTTGTPADCILLGIYEIIKDRVDLVVSGINHGAN
                     VGIDITYSGTVSAAMEGAICGIPSIAVSLATHKDVSGNIFEMAGDFTTKLAELVMKKG
                     LPGDTLLNVNIPPVERPLGVKFTYQGSKRYKPKILKRFDPSGEPYYWLDASFVDNDED
                     GSDLTAIREGYISITPVKIDMTDYNNLEEISSRYKEIESFL"
     gene            1743..2264
                     /locus_tag="P5310_05915"
     CDS             1743..2264
                     /locus_tag="P5310_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32930.1"
                     /translation="MRYAVVVSVILCIALLSWWLWMSSPEREEEEVVSGPKIVIEKGE
                     IIGMYEGRRSFILKADDFKTKTETVAIVDGKIEGTVFDENGEIIASFEGLGGEVDLRN
                     SNFKIYSGGRIKGEGFEVVANTITWNNSSNMFEANGDVNVHLNSYRVKCRDMKADFAA
                     QVIELSGSPVLEF"
     gene            2278..>2534
                     /locus_tag="P5310_05920"
     CDS             2278..>2534
                     /locus_tag="P5310_05920"
                     /inference="COORDINATES: protein motif:HMM:NF015901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HRU32931.1"
                     /translation="MRRLFIFTLCLILIAPTFIYAQKQEASTQGGKLYGDKFIYDMKT
                     KLLKVEGNVRVELKDGPKISGNKLEFNQDKQLMVMPGAINL"
BASE COUNT          778 a          362 c          690 g          704 t
ORIGIN      
        1 tagacctgat aatagtaaat ggggaaaact ctgcaggagg tttcggaata acaagagggg
       61 tagcagagga gctcttttcc ctctcagttg aggtgattac ctctggaaac catatctggg
      121 ataaaaagga aggtatggag ttaattatag aagaagagag aattttaaga ccattgaatt
      181 atccccctga ggtgccaggc agggggtata ctatattaaa cttgccgaat aaagaaaaga
      241 ttggtattgt taatcttatg ggaagggttt ttaccggtgt atctcttgac tgtccattca
      301 gaaaagggat ggaggtagtt gaagagttac ataatgacgg tgtaaaatcc atattgatag
      361 atttccatgc cgaagcgact tcggaaaaga tagccctctg gagatatctg gatggaatgg
      421 tgagtgcagt ccttggaacc catacccatg tccagacagc agatgaagag atatctctac
      481 ttgggactgc atacatatca gatgttggta tggtcgggtc agcagatggg attataggtg
      541 aagataagga tattattata gagaggtttt tgacctctct tcctaggaga tttaaggtag
      601 caaaggggag gaccctcttt aatggtgtgt tgctggagat agatactagt tcaggtaagg
      661 ctatatctat agaaagaatc agaaaatatt ataaggagga ttgaaaatga caacattaaa
      721 ggtttctgca aaatctaatc caaactcagt tgccggagct ctggcagggg tgataagaga
      781 ggaaggtaca gtagaaatgc aggcgatagg ggcaggagcg gtgaatcagg cggtaaaggc
      841 gatagctata gctagagggt tcctggctcc cagtggattg gaaatcatat gtatacccgc
      901 atttgttaat gtggagatag ataatgaaga gaggacagcg ataaagttta tagtagaacc
      961 ccgtaaatga cgatatcctc ccctactatc cttgtatcta acgatgatgg gcttgggact
     1021 accgggctgt ctgtattggg gaggtgtttg agcagattag gaagggttgt agttgtttcg
     1081 cctgataggc agagaagtgc tggtgggcat tctatcactc tcagagaacc tataaggatg
     1141 aagagactgg atctggagat agagacctgg gtaactaccg gaacaccagc tgattgtata
     1201 cttctgggga tttatgagat tataaaagat agggtagatt tggtggtctc agggataaat
     1261 catggtgcga atgtaggcat agatatcacc tattccggaa ctgtttcggc tgctatggag
     1321 ggagctatat gtggaattcc ttctatagcg gtttctctgg ctactcataa ggatgtaagt
     1381 ggcaatattt ttgagatggc tggtgatttt acaactaaat tggctgagct ggttatgaaa
     1441 aaggggttac ccggggatac cctgttaaac gtaaatatac cccctgtaga gagacctctg
     1501 ggggttaagt ttacatatca gggttcaaag aggtataagc caaagatact gaagagattt
     1561 gacccgtccg gagaacctta ctattggctt gatgcttctt ttgttgataa tgatgaggat
     1621 ggcagtgatc ttaccgctat aagagaaggg tatatatcta taactccagt taaaatagat
     1681 atgacagatt ataataatct ggaggagata tcatccaggt ataaagagat tgaatcattt
     1741 ttatgagata tgcagtagtg gtatctgtta tcttatgtat agccttactg agctggtggt
     1801 tatggatgtc ttctccagaa agggaggaag aagaggtggt atctggacct aagattgtga
     1861 tagaaaaggg tgagattata ggtatgtatg agggtagaag aagcttcata cttaaggcgg
     1921 atgattttaa gactaaaaca gagactgttg ccattgtgga tggtaagata gaagggacgg
     1981 tctttgatga aaatggggag ataatagcct ctttcgaagg tcttggtgga gaagtagacc
     2041 tgagaaacag taactttaag atctattccg gggggaggat aaagggagag ggattcgaag
     2101 ttgtagcgaa taccataacc tggaataata gcagtaatat gtttgaggca aatggtgatg
     2161 taaatgttca ccttaattct tatagggtaa agtgcaggga tatgaaagcg gattttgcag
     2221 cccaggtgat agaattatcc ggcagtccgg tattagaatt ctagttaagg aggatttatg
     2281 cgcaggctat ttatctttac actgtgtttg atattaatag ctcctacctt tatttatgca
     2341 cagaaacagg aagccagtac tcagggtggt aagttatacg gggataagtt tatctacgat
     2401 atgaagacga agcttttgaa ggttgagggt aatgtcaggg tagaacttaa agatggtccc
     2461 aagatctctg ggaataaact ggaattcaat caggataaac aattgatggt tatgcctggt
     2521 gcaataaatc tgga
//
LOCUS       DAOTKU010000234         2527 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_33422_length_2527_cov_3.849110, whole genome shotgun sequence.
ACCESSION   DAOTKU010000234 DAOTKU010000000
VERSION     DAOTKU010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33422_length_2527_cov_3.849110"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /gene="rseP"
                     /locus_tag="P5310_05925"
     CDS             <1..662
                     /gene="rseP"
                     /locus_tag="P5310_05925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HRU32932.1"
                     /translation="VPTGINTVLPGSPAEMAGIKPGDRIISVNGKEVTLSEEITQAIQ
                     SSKDKVILEIERGGERLTIELIPKYDEVQKRNLIGITIPAFTDVRYPVGEAFIMSLKE
                     FSTIIVGTLEAFGGLITGKLGSEAISGPIGVARITGAAAQMGIKVFLQLMAFLSIQLG
                     LFNLLPIPALDGGRIFFLILNLGKKIKISPEKENLVHYIGFLVLLILMIIVTIGDVSR
                     F"
     gene            662..1714
                     /gene="ispG"
                     /locus_tag="P5310_05930"
                     /gene_synonym="gcpE"
     CDS             662..1714
                     /gene="ispG"
                     /locus_tag="P5310_05930"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HRU32933.1"
                     /translation="MWKRDDTLPIKVGNLTIGGGNPVAVQSMTNTKTEDVSATVSQIK
                     RLEDAGCEIVRVAVPTREAALKLREIKNNISIPLIADLHFDLSLGFIALEMGVDKIRI
                     NPGTVPSREILRSLVLEAKDRNIPVRVGVNKGSLPDGYQPTKDGMVQCVLDYVKFIED
                     LGYNNLVVSAKSSDPEETIEANKILSSQVRYPISLGVTEAGGGWRGIVKSTVGLALAL
                     NDGIGDTVRVSLTGDPVMEVKVAYEVLKNLKLRRYGVNLIACPTCGRCQVDLESYYLE
                     VENALEDVIIPVDVAIMGCSVNGPGEAKLADCGVAFGKDKAIFFIKGESMGTFSPGDA
                     VKRLIDYVKEEDKRSR"
     gene            1717..>2527
                     /gene="proS"
                     /locus_tag="P5310_05935"
     CDS             1717..>2527
                     /gene="proS"
                     /locus_tag="P5310_05935"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HRU32934.1"
                     /translation="MSQLFYYPQKEIPKDVEAISHILMVKSCMVERVASGIYDFLPLG
                     FRVLKKIEDIIRREMLSIGAQEVLLPALHPSELWKITGRWDDYGEELFKLTDRHNRTL
                     CLGPTHEEIITTLVKAKINSYRQLPFMLFQIQTKFRDEPRPRFGIIRCREFIMKDLYS
                     FHADEDSLNDGYYKVYYAYKNIFDAIGANYKIVEAASGPIGGDVSHEFVIIAESGEDE
                     IAYCSSCSYSATTEMADYNPVIVEDNESELPLEKIHTPGLKSVEEVSGYLNV"
BASE COUNT          782 a          390 c          626 g          729 t
ORIGIN      
        1 ttgtccctac aggtataaat acagttttgc caggttctcc agctgaaatg gcaggaataa
       61 agcctggaga taggattata tctgtcaatg gtaaagaggt taccctctct gaggagataa
      121 ctcaggcaat acagagttca aaagataagg tgatactgga gattgagagg ggtggagaaa
      181 gattaaccat tgaattgatt ccaaaatacg atgaagttca gaagagaaac cttattggaa
      241 taacaatacc tgcttttact gacgttagat atcctgtagg tgaggcattc ataatgtctc
      301 tgaaagaatt ttctactata attgttggca ctttagaggc ttttggaggc ttaattaccg
      361 gtaaacttgg ctcagaagct atcagtggac ccataggagt tgccagaatt acaggggcag
      421 cagctcagat ggggataaaa gtattcttgc aacttatggc atttttgagc attcaattgg
      481 gtttattcaa tcttctaccc atacccgcac tggacggggg tagaattttc ttcttaattc
      541 tgaatcttgg caagaagata aagatttccc cagagaaaga gaatcttgtt cactacatag
      601 gttttttagt cctgctgatc ttgatgataa tagttactat aggagatgtg agccgcttct
      661 gatgtggaaa agggatgata ctcttcctat aaaagttggg aatctgacta taggtggagg
      721 aaatcctgta gcggttcagt cgatgaccaa taccaaaacc gaggatgtat cagctacagt
      781 tagccagata aagagattgg aagatgctgg atgtgagatt gtaagagtag ctgtaccgac
      841 aagagaagct gcccttaaac tcagagagat aaagaataat atatctatac ctcttattgc
      901 tgaccttcac tttgatctat ctcttggttt tatagcccta gaaatggggg ttgataagat
      961 caggataaac ccagggactg ttcccagtag agagatatta agaagcctgg tcctggaggc
     1021 taaagataga aatattcctg tcagggttgg tgttaataag ggttccttac cagatggata
     1081 tcagcctact aaagacggga tggttcagtg tgtgctggat tatgtaaaat ttatagagga
     1141 tttgggatat aataatcttg tggtttctgc aaaatcttca gatccagagg aaaccataga
     1201 ggctaataag attttatctt ctcaggtcag ataccccatt tctcttggtg ttacagaggc
     1261 tggaggagga tggagaggta tagtgaagtc tactgtaggg ctggctctgg ctttgaatga
     1321 tggtatcgga gacactgtaa gagtatcgct tacaggagac cctgttatgg aggtaaaagt
     1381 tgcctatgag gtccttaaga atttaaagct gagaaggtat ggtgtaaatc tgattgcctg
     1441 tcctacctgt ggaagatgcc aggtagattt ggagtcttac tatctggagg ttgaaaatgc
     1501 cctggaagat gttatcatac ccgtagatgt tgctattatg ggttgtagcg taaatggacc
     1561 aggggaagct aaacttgccg attgtggagt tgcatttgga aaggataagg caatattctt
     1621 tataaaagga gaatcaatgg gaacatttag tcctggagat gctgttaaaa ggcttattga
     1681 ttatgtaaag gaggaagata aaaggtccag atgagaatgt cgcagctttt ttactatcca
     1741 caaaaggaga ttcctaagga tgtagaggct ataagccata tccttatggt taaaagttgt
     1801 atggtagaga gggtggcttc agggatatac gattttttac cgttaggttt tcgggttctt
     1861 aaaaagatag aagatataat caggagagag atgttatcga ttggtgctca ggaggttctc
     1921 ctacctgccc ttcatccaag cgagttatgg aagataaccg gaagatggga tgactatgga
     1981 gaagagctat ttaaactcac agatagacat aatcgtacat tatgtctggg tcccacgcat
     2041 gaggagatta taaccaccct ggtgaaggct aagattaatt cttaccggca acttccgttt
     2101 atgttatttc agatacagac taaattcagg gatgagccca gaccaaggtt tggtattata
     2161 agatgtagag agttcataat gaaagatttg tattcttttc atgcagatga ggacagtctt
     2221 aatgatggat attataaagt atattatgcc tataaaaata tattcgatgc gataggagct
     2281 aactataaga ttgtagaggc agcaagtgga cctataggtg gagatgtatc ccatgaattt
     2341 gtaataatag cagaaagcgg ggaagatgag atagcgtact gttcttcctg tagttactca
     2401 gccaccaccg aaatggcaga ttataatcca gtgatagttg aagataatga gtcagagtta
     2461 cctttagaaa agattcatac tcccgggtta aagagtgtag aagaggtatc tggatatcta
     2521 aacgtcc
//
LOCUS       DAOTKU010000235         2527 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_33425_length_2527_cov_2.687702, whole genome shotgun sequence.
ACCESSION   DAOTKU010000235 DAOTKU010000000
VERSION     DAOTKU010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33425_length_2527_cov_2.687702"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="P5310_05940"
     CDS             <1..196
                     /locus_tag="P5310_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HRU32935.1"
                     /translation="VVSAFGTVGLSTGITPSLSTVGRIVIILVMYLGRVGLVVFGLSF
                     LYPLRRKSHIELPYGEVSIG"
     gene            193..864
                     /locus_tag="P5310_05945"
     CDS             193..864
                     /locus_tag="P5310_05945"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HRU32936.1"
                     /translation="MKFPWFQDFNKPIQYAVIGIGRFGTSLVETLIGLGYEVLAIDPE
                     EKNVERVRNIATYSAVIDVTDEKELHDAGITNVDVVVVCLSDLEMNILATLIVKELGI
                     KYVIARGLNDNHVEILKLIGADWIVCPEKEMGKKIAQKMVIPGIIDLMDAIPEARLIE
                     IVPTPDLVGKRIRELTLSRNGILVLGIRRDGNAIIVPNPEEIVRASDRIYLFGSPSVL
                     EDFII"
     gene            982..1668
                     /locus_tag="P5310_05950"
     CDS             982..1668
                     /locus_tag="P5310_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HRU32937.1"
                     /translation="MRISNREIALGGLFGGITLLLGFTYLGYIPLPTPAGAATIMHIP
                     VIIAGLLLGPRFGFIVGSLFGLSALYYFLSVAPPWVLFPARPFIGVVPPLVYYSMLRL
                     LEGKEKSYIRWISLIISSILLVITLGLTYTKLGISFWFILLCLLLIVIILFWVRSSDP
                     KIVSLAVASFLGSMTNTIGTLGLATIFKIYPFSVFISLSILQGIPEALLAVIICTPIV
                     IAIEGRVKKV"
     gene            1665..2429
                     /locus_tag="P5310_05955"
     CDS             1665..2429
                     /locus_tag="P5310_05955"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HRU32938.1"
                     /translation="MIIAIDVGNTNIDVAFFQGYKLLRHFEFSTNRQATSYEYAINLS
                     WLINNNGLKADDVEGAALASVVPPVTVELERAIRYLFDIKPFIVVPGIRTGLIIDVEN
                     PKEVGADIICNAVSLVEDYRSSGIVIDFGTAITVLGVKERKLLGVAIAPGIWTASSAL
                     WERAAKLSPIDIFNPPESVLGKNTVTAMQSGIIYGFIGLVDKLVSLELEEMEIEPFVV
                     ATGGWSERIARYCNTINLVDPYLTLRGLCRLYMKNI"
BASE COUNT          733 a          411 c          566 g          817 t
ORIGIN      
        1 ggttgtatct gcatttggta cggttggttt atctacaggt ataaccccat ctctttccac
       61 agtgggaaga atagtaataa tcctggttat gtatctgggt agagttggtt tagtagtctt
      121 tggcttatca tttttatacc ctttaagaag aaaatctcat atagagttac cttacggtga
      181 ggtgagtata ggatgaagtt cccttggttt caggatttta ataaacctat ccagtatgca
      241 gttataggta taggaagatt tggaacatca cttgtagaaa cactgatagg tttgggttat
      301 gaggtcctgg ctatagatcc tgaagagaag aatgtagaaa gggtaagaaa tatagctact
      361 tattcagcgg tgattgatgt taccgatgag aaagaattgc atgacgcagg aatcactaat
      421 gttgatgtgg ttgtggtctg tctctcagac ttagagatga atatacttgc gacattgata
      481 gtaaaagagc ttggaataaa atatgtaata gccagaggac tcaatgataa ccatgtggag
      541 attttaaaac ttatcggtgc agactggatt gtatgtccgg aaaaagagat gggaaaaaag
      601 attgctcaga agatggttat tccggggata atcgacctga tggatgctat cccagaagcc
      661 agacttattg agattgttcc cactccagat ctggttggta aaaggatcag ggagcttaca
      721 ctttccagga atggaatact tgttttaggg ataagaagag atggaaacgc aataattgtt
      781 cctaaccctg aagagattgt aagagctagt gatagaatat acctatttgg atctccttct
      841 gtacttgaag attttatcat ttaatggtat attattagct caagtggctt gtacctgata
      901 gacaagacca ctctgtttat ttaagattac aaattaaaat tcgtccggta ccatatcgga
      961 ctggaacgga ggtgttttta aatgcgtatc tctaatagag agattgcttt aggcgggtta
     1021 tttggaggta tcactcttct tcttggattt acctatctgg ggtatattcc attacccact
     1081 ccagctggag cagctactat catgcatatc cctgttataa tagcaggttt acttctggga
     1141 ccgagatttg gatttatagt tggttctcta tttggtttat ctgccctcta ttactttttg
     1201 agtgtagctc ccccctgggt cctgttcccc gccagaccat ttataggtgt agtaccacct
     1261 ctagtctatt attctatgct tagacttctt gagggaaagg aaaagagtta tataagatgg
     1321 atctctctta tcatctcttc catcctgtta gtaattacct taggactcac ctatacaaag
     1381 ctgggcataa gtttttggtt tatactcctc tgcttgcttc taatagtaat tattcttttc
     1441 tgggtgagaa gttcagaccc caagatagtt tctcttgctg tagcctcttt tctaggtagt
     1501 atgaccaata caattgggac attgggttta gcgacgatct ttaagatata tccattttca
     1561 gtatttatct ctctatcgat tcttcagggt ataccagagg cccttctggc agtaataatc
     1621 tgtacaccaa tagtaattgc aattgaaggg agagtgaaga aggtttgata atagcgattg
     1681 atgtggggaa taccaatata gacgtcgctt ttttccaggg gtataaacta ttaaggcatt
     1741 ttgaattttc taccaacagg caagctacaa gttatgagta tgctatcaat ctatcctggc
     1801 ttataaataa taatgggttg aaggctgatg atgttgaagg tgccgcttta gcatcagtcg
     1861 ttcctcctgt aacggtagaa ttggagagag caataagata tttatttgat ataaagccct
     1921 ttatagttgt tcctggcata agaacaggat tgattataga tgtagagaat cctaaagagg
     1981 taggagcaga tataatttgt aatgcggtat ctcttgttga agattataga agttccggta
     2041 tagtaataga ttttggtacc gctatcactg tcctgggtgt taaagagaga aaattactcg
     2101 gagtagccat agcaccgggt atatggactg caagttctgc cctatgggag agagctgcca
     2161 agctctctcc catagatatc tttaatccac ctgaaagtgt ccttggtaaa aatacagtca
     2221 ccgctatgca gtcaggtata atatatggtt tcattggatt ggtagataaa ttagtttctt
     2281 tagaactgga agagatggag atagaaccct ttgttgtggc tactggtggc tggtcagaga
     2341 gaatagcgag gtactgcaat actattaatc ttgtcgaccc ttatcttact cttagagggc
     2401 tttgcagatt atatatgaaa aacatataga tgattgactt atcctattcc tttttcatat
     2461 actaaaggag tggatataga gacatttaga cagggaaaaa aatggaaaga gtcaatagct
     2521 cttagga
//
LOCUS       DAOTKU010000236         2503 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_33837_length_2503_cov_3.787173, whole genome shotgun sequence.
ACCESSION   DAOTKU010000236 DAOTKU010000000
VERSION     DAOTKU010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33837_length_2503_cov_3.787173"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            14..109
                     /locus_tag="P5310_05960"
     CDS             14..109
                     /locus_tag="P5310_05960"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HRU32939.1"
                     /translation="MYISKKREIPVMREVKKVFDPNGIMNPGKIF"
     gene            410..1633
                     /locus_tag="P5310_05965"
     CDS             410..1633
                     /locus_tag="P5310_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HRU32940.1"
                     /translation="MRAINIKSMHESNISLILNIIRTSDGISRYEISKITRLSPSAVS
                     NVVDLLIEEGFVSETPGETKNVGRRPMRLSLNNDVHFPVGIEIENDKITGVVINLAGE
                     MVKQTTKYISTDMDHNKILEEVIDVFYLLSQDFSPEQIMGLGIAIPGIVDSKNGISIL
                     SENLGWRNVAVKEILKTKIDVPMFIEQNIRAVTLGELWYGSGIGKNNLICVRVGSGVS
                     AGFVINGSIYRGSAGIVGELGHTVVESDGNRCRCGSQGCLESYVSTQVLISKVLEGLN
                     NRIYSQVSLEGKNRDEQLEEIFSAGKKGDRFILNIFEEMGKYLGIGISNLINLFNPEL
                     VVIAGGLSRAEELLLEPVIRTVKLHTFPPIPEFSIPEITISKLGAFTCATGVATFVIE
                     DVFLHPEKKLRRIKV"
     gene            1630..2313
                     /locus_tag="P5310_05970"
     CDS             1630..2313
                     /locus_tag="P5310_05970"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HRU32941.1"
                     /translation="MIENWQRILVFSAHPDDEILGVGGTIAKLSKSGKEVYVVTFAIG
                     ETSYSSIEMKEKMRELRLKEAEECNKVLGIKERIILYKPNQGITNDRETYQECVKIIR
                     KYKPDVIFTHYKEDKHRDHRAVWEITDEARWKAAENVLADFGTPWYTPELYYYEVIEL
                     FTYPSLLIDITDTLERKVEAMKTQSSQFPVLPGVIEYIEGLGKVRGYLRGTKYAEAFL
                     SSNFIARLE"
BASE COUNT          869 a          353 c          594 g          687 t
ORIGIN      
        1 aaaaaagaag gcgttgtata tatcaaagaa gagagagatt cctgtgatga gagaggtcaa
       61 aaaggttttt gaccctaacg gaattatgaa tccagggaag atcttctaga taatatatct
      121 cattttttct ttttaagaaa atataggtac tgtcaatagt ctgtaaaaat gtcaggggaa
      181 ggaaataaaa catatgagat taatttgtat cccgtagggg caattcatga attgccccta
      241 caatttcgtc ttttataata aaaaccccat gcctttatcc ttcctccccc acctcggggg
      301 aggggaatag gtcagaacta tttacccatg acaatatcat agaacgttaa caggtacttg
      361 acactggtaa aaaatcgata tataatttct ttgtgaggaa aaattatata tgagagcaat
      421 aaatattaaa agtatgcatg aatcgaatat atcactgatc cttaacataa taaggactag
      481 tgatggtatc tctcgctatg agatttcgaa aattacaaga ttaagtcctt cagcagtatc
      541 caacgttgtt gatcttctta ttgaagaagg ttttgtctca gaaactcccg gggagacgaa
      601 aaatgtaggt agaagaccta tgagactatc tttaaataat gatgtacact tccctgtggg
      661 tatagagata gagaacgata agataacagg agtagtgata aacttagctg gagagatggt
      721 aaagcagact acaaaatata tttctacaga tatggatcat aataaaatat tggaagaagt
      781 aattgatgtg ttttatctcc tatcacaaga tttttctcct gagcagatta tgggtctggg
      841 gattgctatc ccaggcattg tggacagtaa aaatggcatc tctatacttt cagaaaatct
      901 aggttggcgg aatgtagctg taaaggaaat tcttaaaaca aagatagatg tccccatgtt
      961 tatagaacaa aatataagag cggttactct tggagaatta tggtatggat caggcatagg
     1021 gaagaataat cttatatgtg ttagggttgg tagtggggta agtgcaggct ttgtaataaa
     1081 tggaagtatc tatagaggtt cagctgggat agtgggtgaa ttaggtcata cggttgtaga
     1141 atcagatgga aatagatgca gatgtggcag tcagggatgt ctagaaagtt acgtctccac
     1201 tcaggttctt atcagtaagg ttctggaagg attaaataac aggatctatt ctcaggtttc
     1261 attagaaggt aaaaatagag acgagcaact ggaagaaatt ttttctgcgg gtaaaaaagg
     1321 agacaggttt atcctgaata tattcgaaga gatgggtaaa tacctgggta taggtatctc
     1381 taatcttata aatctgttta accctgagtt agttgtaata gcaggaggtc tatcaagagc
     1441 agaggaactt ttacttgaac cagttataag aacagttaaa ctccacacat ttcctcctat
     1501 tccagaattc agtatacctg agattaccat atctaaactg ggagctttta cctgtgccac
     1561 gggtgtagct acttttgtca tagaggatgt atttttacat ccagagaaaa aactgaggag
     1621 gattaaggta tgatagaaaa ctggcaaagg atacttgtat ttagtgccca ccctgatgat
     1681 gagatactgg gggttggagg gacaatagct aaactctcta aatcaggcaa agaggtctac
     1741 gtggttacct ttgctatagg agagacaagt tatagctcta ttgaaatgaa agagaagatg
     1801 agagagttga gattaaaaga agctgaagaa tgtaataagg tcctggggat aaaggagagg
     1861 ataatactat ataaaccaaa tcaggggatt acaaatgata gagaaacgta tcaggaatgt
     1921 gtaaagataa taagaaaata caagcctgat gtaatcttta cacattataa agaagacaaa
     1981 catagagacc atagagcggt ctgggagatt acagacgagg ccagatggaa ggcagccgaa
     2041 aatgttcttg cagactttgg aactccctgg tataccccag agctctacta ctatgaagta
     2101 atagaactct ttacatatcc gtcacttctt atagacataa ccgatactct ggagagaaag
     2161 gtagaagcta tgaagacgca gagtagtcaa ttccctgttc ttccaggggt tattgagtat
     2221 atagagggtt taggaaaagt acgaggttac ctgaggggga caaaatatgc agaggcgttt
     2281 ctgtcttcaa actttatagc aaggctggag taggggggga aaacctacag attgacttgt
     2341 agggattaca gggataggag ggttgaaaag agttagatag gcgtatcttg acaagtccag
     2401 taagaaagag aggtgatgtt tagagaaaaa gatataaaag atataaagga ataaaaaggt
     2461 cagggggttt aattctgggg ggaagaagtg tgggtaaggc tat
//
LOCUS       DAOTKU010000237         2491 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_34048_length_2491_cov_3.414204, whole genome shotgun sequence.
ACCESSION   DAOTKU010000237 DAOTKU010000000
VERSION     DAOTKU010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34048_length_2491_cov_3.414204"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1120
                     /locus_tag="P5310_05975"
     CDS             <1..1120
                     /locus_tag="P5310_05975"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRU32942.1"
                     /translation="TNKINISRAKAISATFIVLESILIIISFSNSKNEFPDIYYRGMY
                     ALILAAMIVYLLVFSKLEGNISANKTIIEVSGISFACILLYWCMGISLLDQLSYGQII
                     VYISALLAIAVVPLFSPLVSLFLYCSSQILFIALLPYFQQSSSVLYGHYVNSSTLAVL
                     SWIISRIRYVSFVEDFEYKRVIQEKNDELAKVNKELEEANRILKELSQIDGTTGIFNR
                     SVFDQVMLTEWNRCRRHSIPLSLIIIDIDFFKTFNDNYGHLAGDDCLRQVAKLLKSCA
                     KRSSDTVARYGGDEFTIILPHMEKEDAVNFAEQLRKRVLKEITIPHEYPSILTHLTIS
                     LGVATIIPSSESSIKEFINAADQALYEAKKERNKVVVM"
     gene            complement(1122..2480)
                     /locus_tag="P5310_05980"
     CDS             complement(1122..2480)
                     /locus_tag="P5310_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32943.1"
                     /translation="MSIKILLWYDVEDYITTESEDALLELIKMMDLRDIRGTFKLVGE
                     KVRRLKEHGRTDILERLKRHEIGYHTDFHSQHPVVSEYLEHSGFKDGAIEFERRELQG
                     LKDIINITGMMPVTYGQPGSAWAPQVYPALLKWDIHVYLDVYDFISVDKKPFWFGGVL
                     NITDPQGVLRMELVEDGLEKAKRSYDILYEKAPEDRTEIVSIYYHPCEFATKDFWDKY
                     NFSYGINTPEEKWKKPPLRSKEEMRFYIERLGDFLDYIISKGDVDFVTAGKILHYEIK
                     NETPVTGEEVKRLASNIYNELSFDISDNRTLSASEIFSIFSRYLNGQELTPELIYGPE
                     NQVDTEVNERINVHKLIEATNIEYPDVFGHKQLPDYFITDKGRINPVDMTCTLAKIIR
                     EDLREDDEISIIRGTLKSSHYINEDDNLGKKWIIFPTEFRAPNIIRTAKLQSWTLKPA
                     MF"
BASE COUNT          707 a          510 c          424 g          850 t
ORIGIN      
        1 tacaaataag attaatataa gcagagcaaa agcaatttca gccactttta ttgttttaga
       61 aagtatactg atcatcatat ctttctctaa cagcaagaac gaattccccg acatttacta
      121 tcgcggtatg tatgctttaa tacttgccgc gatgattgtt tatctattgg tatttagtaa
      181 attggagggg aatatatctg ccaataagac aattattgag gtcagtggta tatcctttgc
      241 ctgtatttta ttgtactggt gtatggggat atctttgtta gaccaattat cttacggaca
      301 gattatagtc tatatttcag cactgttagc gattgcggta gtccctcttt tttcacccct
      361 ggtttcactc ttcctatatt gttctagcca aatccttttt atcgctctcc taccctattt
      421 ccagcaatct agctcagtcc tctatggaca ttacgtaaac agctctacct tagcagtact
      481 atcatggatc atatcgcgta taaggtatgt tagttttgtt gaggacttcg aatataaaag
      541 agttattcag gaaaagaacg atgagctagc aaaagtaaac aaagagttag aggaggcaaa
      601 tcgaatctta aaggagcttt cccaaattga cggaacaacc ggcatcttca atcgctctgt
      661 ttttgatcag gttatgctca ccgagtggaa taggtgcaga cgtcattcta tacctttatc
      721 cctaataata atagacattg attttttcaa aacatttaat gataactatg gacatctggc
      781 cggagatgat tgcctcaggc aggttgcgaa attactgaaa tcctgtgcaa aacgatcctc
      841 agatactgtg gcaagatacg ggggggatga attcacaatc atacttcctc atatggagaa
      901 agaagatgca gtaaactttg ccgaacaatt aagaaaaagg gtcctgaagg agattactat
      961 tcctcatgaa tacccctcca tactcacgca tttaaccatc agcttagggg tagcaacaat
     1021 aatcccttct agcgaatctt caattaaaga atttattaat gccgctgatc aagcgcttta
     1081 tgaagccaaa aaagagcgca ataaagttgt ggttatgtaa cctaaaacat agcaggtttc
     1141 agcgtccagg actgtaattt ggctgttctt ataatgttag gggctctaaa ttctgtggga
     1201 aagattatcc attttttacc caaattatcg tcttcattta tataatggct ggatttaagt
     1261 gtccctctaa ttatgctaat ctcatcatct tctcttagat cttctcttat aatcttagct
     1321 aaagtacagg tcatatctac aggatttatt ctccctttat ctgttataaa ataatctgga
     1381 agttgtttat gaccaaagac atcaggatat tctatattag ttgcttctat aagtttatgg
     1441 acattgattc tttcattgac ttctgtatct acctggtttt caggtccata tataagctca
     1501 ggggttaatt cttgcccgtt tagatatctg gagaatatag aaaatatctc tgaggctgat
     1561 agcgtacgat tatcacttat atcaaagctc agttcgttgt atatattgga ggctaatctt
     1621 tttacctctt ctccagttac aggagtttca tttttaatct cgtaatgaag aattttacca
     1681 gcagtaacaa agtctacatc acctttcgag attatgtaat ctaggaaatc tcctaatctt
     1741 tctatataga atctcatctc ttctttagat cggaggggtg gttttttcca cttttcttct
     1801 ggagtattta tcccataact gaagttatac ttatcccaga agtctttcgt cgcaaactca
     1861 cagggatgat agtaaatgct tactatttca gttctatctt caggagcctt ttcatacagg
     1921 atatcgtagc tcctttttgc cttttctaac ccgtcttcta ccagctccat ccttaatact
     1981 ccctgtggat ctgtgatatt caagacacca ccgaaccaga atggcttttt atctactgat
     2041 atgaagtcat atacatcgag atagacatgg atatcccatt taagtaaagc tggatagacc
     2101 tgaggtgccc acgcagaacc cggctgccca taagttacag gcatcatacc tgttatattt
     2161 atgatatcct tcagcccttg cagttctctc ctttcaaatt ctatggctcc atctttaaat
     2221 cctgaatgtt ccagatactc gcttacaaca ggatgctgac tgtgaaaatc tgtatggtat
     2281 ccaatctcat gtctctttag tctttcaagt atatctgtcc tcccatgctc tttcagtctc
     2341 ctgacctttt ctcctaccag tttgaatgtt cctctgatgt ctcttaaatc catcatcttg
     2401 atcaattcaa gaagtgcatc ttccgattca gttgttatat agtcttcaac atcataccag
     2461 agaagtattt ttatactcat atctttctcc t
//
LOCUS       DAOTKU010000238         2489 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_34082_length_2489_cov_4.106409, whole genome shotgun sequence.
ACCESSION   DAOTKU010000238 DAOTKU010000000
VERSION     DAOTKU010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34082_length_2489_cov_4.106409"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="P5310_05985"
     CDS             complement(<1..352)
                     /locus_tag="P5310_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004239372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRU32944.1"
                     /translation="MTNKKKVTVKYIAEKAGVSYATASLSLSGKGRISESTRKKVLEV
                     ASNLGYIAEDRPASNIPLMAFIDTNSHMGGFYISLIEGVIERLKEDNYAVILDICGEF
                     GPDKIEERKKILERL"
     gene            complement(601..798)
                     /locus_tag="P5310_05990"
     CDS             complement(601..798)
                     /locus_tag="P5310_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32945.1"
                     /translation="MDICKGGLWQRRLKTVEDIPRYWDDIVQLYKAKPVAFSKGGGDG
                     CKPDVDYKILMENFKNSRRLE"
     gene            complement(944..1456)
                     /locus_tag="P5310_05995"
     CDS             complement(944..1456)
                     /locus_tag="P5310_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32946.1"
                     /translation="MRVSLIVSSATNQLGVIKNSTRYLSNTLGITEKELEDRLGQLSD
                     KVLYIKEQNIIYIKNFVKYQAGSVKHINFVKAVKKAFRELPPEVMRKVIEFDPSLESI
                     VVEDDKNKTVSDGEVGNSKYPMDRVSENEKNVGRVSAENKNVDLSVTESVPESVPVSE
                     SVTVSEAQKN"
     gene            complement(1550..2209)
                     /locus_tag="P5310_06000"
     CDS             complement(1550..2209)
                     /locus_tag="P5310_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HRU32947.1"
                     /translation="MIRENVKKILSELPDGVELVAVIKGRPVEQVLEAIDGGVSIIGV
                     NYLKDVNKLYQATGDRVKWHYIGIARFEKHDLLRRKYLEVFDMIETVDSAELAEDFDK
                     KCSQIGKVMPVLIEVNIAREPQKSGVIPENVESLIREVSGLKNIKVMGLMTMGPLVED
                     SESLRPYFRETKRLFDHIKSMEIPNVEMRYLSMGMSDSYRIAIEEGANIVRIGTKLFK
                     E"
     gene            2297..>2489
                     /locus_tag="P5310_06005"
     CDS             2297..>2489
                     /locus_tag="P5310_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HRU32948.1"
                     /translation="MTDKDSSRLKLLLSLFSVFFKIGAFTWGGGYAMLPLIKNEVVEK
                     KRWISSDDFIDGIAVSQSLP"
BASE COUNT          662 a          566 c          395 g          866 t
ORIGIN      
        1 ataaccgctc taagattttc tttctttcct ctatcttatc tggaccgaat tccccacata
       61 tatctaggat tacggcgtag ttatcctctt tcagtctttc tataacccct tctattagcg
      121 agatatagaa cccacccata tgactattgg tatctataaa agccataaga gggatattag
      181 atgctggtct atcttcagcg atatatccaa gattagatgc tacttcaagg accttttttc
      241 tggtagattc acttatacgt ccctttccag agagagatag ggatgcagtt gcatatgaaa
      301 caccagcttt ttcagctata tattttacgg taaccttttt tttattagtc atagttaaat
      361 cttcatttaa cctatttgct aaaaaattag cataatatat taactgtgtc aagcgaaatg
      421 tcgaaattaa acagcttatt tatctctata ttcatctgtc taggtacgcc tatcttatcc
      481 tttaaacctc gatacactgt aaacctgttt atattttacc tctccactgc cctaaagaca
      541 ccattatttt tgtgaaagac aattgcccgc tctccaatcc catacttggc acaggttctc
      601 tcattcaagc ctccttgagt ttttaaagtt ctccataagg atcttatagt cgacgtctgg
      661 tttacagcca tcacctccac ccttggaaaa tgcaaccggc tttgccttat agagctggac
      721 gatgtcgtcc cagtatctgg ggatatcttc taccgtcttt agtctcctct gccagagccc
      781 tcctttacag atatccaata ggaccctggc aacctctacc ctgtcagcgg gatatattct
      841 tttatctttt cagctagggt gtcgtctgta ttaccggagt tcttattcaa gatagctatt
      901 atcttttctg ttgcttcttt ctctctagtt gaaaaattaa aatttaattt ttttgcgcct
      961 ctgatactgt tactgattct gatactggta ctgattctgg tactgattct gttactgata
     1021 gatctacatt tttattttct gctgataccc ttccgacatt tttttcattt tcagataccc
     1081 tatccatagg gtattttgaa ttccccactt caccatcaga aacagtttta tttttatcat
     1141 cttctaccac aatgctttca agagaggggt cgaattcgat aacttttctc atcacttctg
     1201 gtggtagctc tctgaacgct ttcttaacag ccttgacaaa attgatatgt tttacagatc
     1261 ctgcctgata ttttacaaag ttctttatgt agatgatatt ctgttctttt atgtataaga
     1321 ccttatcaga cagttgacca agtctatctt caagctcctt ttcggttata ccaagtgtat
     1381 tagagagata tcttgttgaa ttctttatta cccccagctg atttgttgcg cttgagacta
     1441 tcagactcac cctcacccag attctttcct cctcccccat ttcgggggag gatagaggtg
     1501 ggggcttccc gtcaagggag agggagagaa atcctactcc ttaccatatt cactccttga
     1561 aaagtttcgt tcctatcctt actatgtttg ccccctcttc gatggcaatt ctgtacgaat
     1621 cgctcatacc catagaaagg tatctcatct caacattcgg tatctccata ctctttatat
     1681 gatcaaataa tctttttgtt tccctaaaat aaggtctcag actttctgaa tcctcaacaa
     1741 ggggtcccat agtcatcagt cccatcactt ttatattttt aagtccagag acctctctaa
     1801 ttaaggactc cacattttct ggtattaccc cagacttctg aggttctctg gcgatattga
     1861 cctcaatcaa tacaggcatc accttcccta tctgagaaca ctttttatca aaatcttcag
     1921 ccaattcagc agagtcaaca gtttctatca tatcaaaaac ttccagatac ttcctcctca
     1981 gaagatcgtg cttttcaaat ctggcaattc ctatatagtg ccacttcacc ctgtcacctg
     2041 tagcctgata gagtttatta acatccttga gataattcac accaataata cttacccctc
     2101 catctatcgc ctcaagtact tgctcaacag gtcttccttt tataaccgct accagctcca
     2161 ctccatctgg gagctcactt aaaatcttct tgacattctc tctaatcata gactatatta
     2221 taactcatca ataaaggtag tttgttgtat aatagataaa attatcgcag agaagaaagg
     2281 agattggagg aaatttatga cagataaaga ttcttccagg cttaagcttt tattatcatt
     2341 gtttagtgtc ttctttaaga taggtgcatt tacttggggt ggaggatatg caatgctacc
     2401 actcataaaa aatgaggtgg tggagaagaa gaggtggata agttcggatg attttataga
     2461 tggtatagct gtatcacaga gtttacctg
//
LOCUS       DAOTKU010000239         2489 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_34090_length_2489_cov_2.646261, whole genome shotgun sequence.
ACCESSION   DAOTKU010000239 DAOTKU010000000
VERSION     DAOTKU010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34090_length_2489_cov_2.646261"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..786)
                     /locus_tag="P5310_06010"
     CDS             complement(<1..786)
                     /locus_tag="P5310_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HRU32949.1"
                     /translation="MSIISPSRKEELRKLAELARYKVATYGEDIDISVFEEAEEVKEI
                     DELSKLPPEIQDAAISSGIDITEKERAGSFVQVDHSVIYQKIQSAYRGQIEIMSITDA
                     LEKHPELEEYWWKAVRVDQDKYTAHAELYPMHGYFIRVLPGQKVDRAIQACLLLQENA
                     RTQNVHNIIMMEEGSSAQIITGCSIAPKVNEGLHIGISEFYIKKNARLVFTMIHNWAD
                     GFHVRPRSGAIVEGNGIFINNYILLKPVKSIQSFPTAVLEGENS"
     gene            complement(777..1508)
                     /locus_tag="P5310_06015"
     CDS             complement(777..1508)
                     /locus_tag="P5310_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012035738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRU32950.1"
                     /translation="MLTIEDLHVEVAGSPILKGVNLEIKEGETHVLLGPNGGGKTTLL
                     MSIMGLPRYKVTGGKIYFKGEDITSLPPNERAKLGIGIMFQKPPSIRGVKLYQIGEVS
                     LRGKEDGDRIKSYAELLNLTNHIDRDLNYGFSGGELKRSELFQLLCQKPDLVLLDEPE
                     SGVDLDNISLIGNVINELLKKEDKVKSRKSSGIIVTHTGYILEYVNADKGHVLMDGRI
                     ICDGSPRDLFEEVKKHGYGRCTECL"
     gene            complement(1549..2076)
                     /locus_tag="P5310_06020"
     CDS             complement(1549..2076)
                     /locus_tag="P5310_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HRU32951.1"
                     /translation="MITIRLLLTFAKIGLFAFGGGYAILPLIEKEVVYGTHWLTHSEF
                     LNVVTISQLTPGPIAINAATFVGYKEGGILGAIFSTLGVCLPSMILVLTVIRFLKKFE
                     TNEVVNRVIKGLRPAVIALIASAAYSIISGGGISDIKGAGIAIISFLLIRTKKIDPIL
                     VLILSGVSGIVLYML"
     gene            complement(2079..>2489)
                     /locus_tag="P5310_06025"
     CDS             complement(2079..>2489)
                     /locus_tag="P5310_06025"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HRU32952.1"
                     /translation="WISSDDFIDGIAVSQSLPGAIAVNTATFVGHKISGRLGSLVATA
                     GAVLPSVITIIIVAMFFLQFKDLKVVQSFFKGANPAIAALIVSSVLDLGKSALKNRQD
                     IIISIGLLLLLIYFNIHPILAIIISAILGIILKR"
BASE COUNT          658 a          600 c          429 g          802 t
ORIGIN      
        1 cgaattttca ccttcaagta cagctgtagg gaatgactgg atagatttaa ccggttttaa
       61 cagtatatag ttgtttataa atatcccatt accctccaca atagctcctg atcttggtct
      121 tacatggaaa ccgtctgccc agttatgtat cattgtgaat acaagccttg catttttctt
      181 tatatagaac tctgatattc ctatgtgtaa accttcattt acttttggag cgatagaaca
      241 gccagttatt atctgggcgg atgagccttc ctccatcata ataatattat ggacgttttg
      301 cgtcctggca ttttcctgca gcaataggca agcctggatt gccctgtcaa ccttttgccc
      361 tgggagaact ctgatgaaat atccgtgcat cggatataat tctgcatggg cggtatactt
      421 atcctgatcc actcttacag ctttccacca gtattcttcc agctctggat gcttttccag
      481 ggcgtctgta atgctcataa tttctatctg ccccctgtag gcagactgta tcttctgata
      541 tataacactg tgatctacct gtacaaaact tcctgctcgt tctttctctg tgatatctat
      601 cccggaagag attgctgcat cctgaatctc tggaggaagc tttgacagtt cgtctatctc
      661 cttcacctct tcagcctctt caaatacaga tatatcaata tcttctccat aggtagcaac
      721 cttatatctt gccagttcag ccagttttct cagttcttct tttcttgatg gacttattat
      781 agacattctg tacaccttcc atacccgtgt ttttttacct cttcaaaaag gtccctcgga
      841 gacccatcac agatgattct tccatccata agcacatgcc ccttatcggc attcacgtat
      901 tccaggatat atcctgtatg ggtcactatg atgccggaac ttttcctgct ttttacctta
      961 tcttctttct tcaggagctc atttatcacg ttacctatca gagagatatt atccagatct
     1021 acgccagatt ccggttcatc caggagtacc aggtctggtt tctggcataa aagctgaaat
     1081 aactctgaac gcttcagctc tccaccagag aaaccatagt tcaggtctct atctatatgg
     1141 ttagtcaggt taagaagctc tgcataactt tttatcctgt caccatcttc tttgcccctg
     1201 agactgacct ctcctatctg atataacttt actcccctga tacttggagg tttctgaaac
     1261 attattccta tacccagctt tgctctttca ttgggtggta aactggttat atcctcccct
     1321 ttaaagtaga tttttcctcc tgttacctta tatcttggaa gtcccataat actcatcaaa
     1381 agggtggtct ttcctccgcc gtttggtcca aggaggacat gtgtttctcc ctcttttatc
     1441 tcaagattta cccccttcag tatcggggaa ccagccacct caacatgaag gtcttctatt
     1501 gtaagcattt ttccatctcc ttagtaattc aataaagatt atatttaatt ataacatata
     1561 aagaactatt ccagatacac ccgaaagtat cagtaccagt atcgggtcta tcttcttggt
     1621 tcttatcagt agaaaactta ttatcgctat ccctgcacct tttatatcgc ttattccacc
     1681 accgcttatt atggaataag cagctgatgc tatcagtgca atcacagcag gtcttaaccc
     1741 ctttattacc ctgttcacca cctcatttgt ctcaaatttc ttcaagaatc tgataactgt
     1801 taaaacaagg atcattgaag gcaggcagac ccccagtgtg gagaagattg cccccaggat
     1861 tcctccctct ttatatccaa cgaatgtggc tgcatttata gctataggac ctggagtaag
     1921 ctgggatatg gtaacaacgt tcagaaattc gctatgcgtt aaccagtggg taccatatac
     1981 aacctctttt tctataagag gcagtattgc atatccacca ccgaatgcaa ataatcctat
     2041 cttggcaaaa gttagaagga gacgtattgt tatcatcttc acctcttcaa gataatccca
     2101 aggattgctg agattataat ggcaagtata gggtgtatat tgaaatatat cagtaacagt
     2161 aaaagcccta tagatatgat gatgtcctgt cggttcttca gtgcagactt ccccaggtcc
     2221 agaactgaac tgactataag ggcagctatt gcagggttcg ctcctttaaa gaagctctga
     2281 acaaccttca ggtctttaaa ctgtaaaaag aacatcgcca ctattattat agttataact
     2341 gaagggagga ctgccccggc agttgcaact aacgagccca atcttccaga gattttatgc
     2401 ccgacaaatg tagcggtatt caccgctata gctccaggta aactctgtga tacagctata
     2461 ccatctataa aatcatccga acttatcca
//
LOCUS       DAOTKU010000240         2477 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_34304_length_2477_cov_3.327828, whole genome shotgun sequence.
ACCESSION   DAOTKU010000240 DAOTKU010000000
VERSION     DAOTKU010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34304_length_2477_cov_3.327828"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(337..2091)
                     /locus_tag="P5310_06030"
     CDS             complement(337..2091)
                     /locus_tag="P5310_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRU32953.1"
                     /translation="MEYLIKLVKIARPYWKYLIIAGISTLAITGVNLLGPWLIRQLVD
                     IITNIGQYPDARKNIINLTILITLSYIARIIFQFLASYLSHYAAWHLVAHMRSMVYDK
                     LQQLPFRFFQERQTGQLMSRVVNDTANFEMLIAHAVPDLFTNILIIVGVAVILFAVNP
                     TLASLSLIPIPFLVYGSTRFAKQVLPNFREAQRALADLNAELQDNISGIREIQIFNQQ
                     MRELVKIRDRIYRHISALLNALRLSAIFHPTVSFLSSIGTVIVISVGGFMALNGKVSV
                     GDIVGFILYLNMFYQPITALANVIENLEQALAGAERVFEILETEPEIKEKPNPIELKN
                     VRGRVTFEDVTFSYNQGSPVLKNISFDIEPGQMVAFVGPTGVGKTTIMYLLVRFFDPD
                     SGSIKIDGIDIRDVSLKSLHDNISMVMQDVFLFNGTIAENIAYGKEDATMEEIINAAR
                     IACAHDFIESLPDGYDTYIGERGVKLSGGQKQRLAIARAVLKDAPILILDEATSSVDT
                     ETENKIQEAINNLAGTRTILIIAHRLSTVKRADRIIVLKDGEIVEDGRHEELIALKGL
                     YYSLSSGQFVGQDLYNVT"
     gene            complement(2264..>2477)
                     /locus_tag="P5310_06035"
     CDS             complement(2264..>2477)
                     /locus_tag="P5310_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32954.1"
                     /translation="PALPIQKFFYSSVHLINQAVRYYNLGSCIYIPHFRLFQQELAVF
                     GIAVSPCPPVFSRGRTLFAAPFLMVF"
BASE COUNT          699 a          520 c          495 g          763 t
ORIGIN      
        1 agtctaataa tatcatattt tgatcactct gaatatctgc tgataattcg gtatacccct
       61 atggggtatt gacagatttt ctatcttaat ctatactata aatgccgcag gtttccgagg
      121 agtctaagga ccaccagaaa ccagaggtaa cttaagttag tagtttaagt tattaccaaa
      181 actttgtttg gtaaatttgg tagaaatact ggaataccga cagggttttg ggaagtaaat
      241 gagcaaaagc ttaagatacc gaaggtagaa tggagttccg gtggttcgtt ggagatctgc
      301 ggctaattgt ttttagtgca acaggtcctg ttctgcctat gttacattat acagatcctg
      361 cccgacaaac tgaccagaac tcaaactgta gtataaacct tttaaggcta taagttcttc
      421 atgtctccca tcttctacaa tctctccatc tttcaggacg attattctgt cagccctttt
      481 taccgttgat aatctgtgag ctataatgag gattgttctc gttcctgcca gattatttat
      541 cgcttcctgt atcttgtttt cggtctctgt atcaacactt gacgtggctt catcaaggat
      601 aagtatgggg gcgtccttca aaactgcacg tgcaattgcc agtcgctgtt tttgaccacc
      661 agaaagcttg acacctcttt ccccgatgta ggtatcatat ccgtcgggaa gactctcgat
      721 aaaatcatgt gcacaggcaa tccttgcagc atttattatc tcctccatgg tagcatcttc
      781 ttttccatat gcgatatttt ctgctatcgt tccgttaaag agaaatacat cctgcatcac
      841 catacttata ttatcgtgga gagatttcag cgagacatct cttatatcaa taccgtctat
      901 ctttatactt ccagagtccg ggtcaaagaa tctgaccaga agatacatta ttgtagtctt
      961 tcccacgcca gtaggtccaa caaatgcaac catctgcccc ggttctatat cgaatgagat
     1021 gttttttaaa acaggggaac cctgattata agaaaaagtt acatcttcga acgttaccct
     1081 gcccctgaca tttttaagct caatcggatt gggcttctct ttgatctcgg gttccgtctc
     1141 taggatttca aagacccgtt cagccccagc aagtgcctgc tccagatttt caataacatt
     1201 ggccaatgct gttatcggtt gataaaacat attgagataa agtatgaatc caacgatatc
     1261 tcccacagag accttcccat ttaatgccat aaagccacca acagagataa caattactgt
     1321 cccaattgag ctcaaaaaac tcacggttgg atggaatatt gcgctcagtc tcagtgcgtt
     1381 caaaagggca gatatgtgtc tgtagattct atctctgatc ttcaccagtt ccctcatctg
     1441 ctgattgaat atctgaatct ctcgaatgcc ggatatgtta tcctgtaatt cagcatttaa
     1501 atcggcaaga gctctctgcg cctctctaaa gtttgggagt acctgctttg caaatctcgt
     1561 acttccatac accagaaacg gtattggtat aagagataga gatgccaggg ttggatttac
     1621 tgcgaagagt atcacggcaa caccgactat tataagaata tttgtaaaga ggtctggaac
     1681 agcatgggct attaacatct caaagttggc tgtatcgttt acaaccctgg acataagctg
     1741 tccggtctgc ctctcctgaa agaatctaaa cggtagttgc tgaagtttat cgtataccat
     1801 acttctcata tgggcaacca gatgccatgc ggcatagtgg gatagatagc ttgctaaaaa
     1861 ctgaaagatt atccttgcta tgtatgagag tgtgatcaga atggtaagat ttattatatt
     1921 tttccttgcg tccgggtatt gccctatatt tgtaatgata tcaacaagct gtcttataag
     1981 ccagggacca agcaagttta cccctgttat ggctaaagtg cttatcccag caattatcag
     2041 atacttccag tatggtcttg ctatctttac cagtttgatt aaatattcca cagtttatcc
     2101 tccagaagat atcatgggtt taatacatat aatattttat cagattttat aagtttttgt
     2161 gaggaatacc cctgacttct atgagtcagg ggtctcttat tgtctgcgtt cccagcagaa
     2221 accattgacg gtttgttgca ataatgctaa attttataaa ggttcaaaaa accattaaga
     2281 atggagcagc gaagagagtc ctgcctctgg agaaaacagg aggacagggg ctaacggcta
     2341 ttccgaatac cgccagctcc tgctggaata gcctgaaatg cgggatataa atacaggagc
     2401 ccaggttgta atatcgtaca gcctggttga tcaggtgaac tgatgaatag aaaaattttt
     2461 gtatcggtaa tgctggt
//
LOCUS       DAOTKU010000241         2472 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_34383_length_2472_cov_3.538684, whole genome shotgun sequence.
ACCESSION   DAOTKU010000241 DAOTKU010000000
VERSION     DAOTKU010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34383_length_2472_cov_3.538684"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            720..884
                     /locus_tag="P5310_06040"
     CDS             720..884
                     /locus_tag="P5310_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32955.1"
                     /translation="MAGLGLDIPEQKMTWKEAYDFGWTLANRVKYISLVSKGISKVGQ
                     ENVYRLALTN"
     gene            1060..1320
                     /gene="spoVG"
                     /locus_tag="P5310_06045"
     CDS             1060..1320
                     /gene="spoVG"
                     /locus_tag="P5310_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HRU32956.1"
                     /translation="MNITDVRIKKVTSQEGKVKAFASITFDDAFVVHGLKVIDGKNGY
                     FVAMPRRDVGNGEFKDIAHPINNQMRENIQSAVLEAFNKSQD"
     gene            1327..1401
                     /locus_tag="P5310_06050"
     tRNA            1327..1401
                     /locus_tag="P5310_06050"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1359..1361,aa:Gln,seq:ttg)
     gene            1423..>2472
                     /locus_tag="P5310_06055"
     CDS             1423..>2472
                     /locus_tag="P5310_06055"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HRU32957.1"
                     /translation="MIAGVVLAGGVGERMNSSIPKLFHNLLGKPIIRYVLENLSGLAL
                     NKIIIVVKSDLKEKFQYTCGDDFVYVVQDKPLGSGDALKRAIPELENFSTVLVVNGDA
                     PLITTEILESLISSHNADNVSATVLSTVIDDPMHYGRVVKDELGNVIRIVEAKDATER
                     EKNIKEVNTGAYCFSVDDLRLVIDRMTNNNAQGEYYITQLIEILSSMGKRVNSYITDL
                     GSLALGINSRFDLSLAIEKLLTEKMEELMQGGVTIVSPENTYIEPDVKVGRDTVIEPF
                     SVLKGNTVIGENCTIGPSSYLLNDDIGDRCEVFYSVVKNSKIESDVRVGPFAHIRDGA
                     IIKSNAAIGDFVEVKK"
BASE COUNT          790 a          362 c          610 g          710 t
ORIGIN      
        1 gaaacttaag aggaaaatat ccttcattga aaccaacttg ctactgcttt tgaaatagag
       61 gctgatgctt ttcttacaaa tgatgagaaa cttaaaaaga tagaggggct aaaatcttag
      121 ttttaaagga ttatttatag taactgttgc aaaataattg acatatttag agctcttggt
      181 tgggaggtat tggatgaata ttgagcaagg actagacagg tctctgtttg aggtgagagc
      241 ccagtcaggt gaatcggaca tggtggtatg ggcgtgggga agctgaaaat ttgtggtcta
      301 taggaactgt tggtatagct cctgttccag tagtagttaa aaataatctg aggaatgttc
      361 cagaaaagag ctttcgagaa tgggtatgtt tacagattag tcataccaga acagaacagt
      421 tctttttcaa aaagtaaatg ataattttgt aataggaggt atctttgata ttaaaagatg
      481 aactaacatc atttgcaaaa gagataggat tagatttagt tggcatatct cctatagaaa
      541 gatttgatgg ggtatcacct gggatgggag attcctagag tacattcagg gacatagaag
      601 gggggacatt atggatgaga gctccaaggc agatagcccc aagatatata tatgattgcg
      661 agatttttag aagataaagg atgggaagtg gtccctgtga cccccctccc tccagagaga
      721 tggccggact gggtctggat attccggagc agaagatgac atggaaagaa gcttatgact
      781 ttggctggac cctagctaat agagttaagt atatctcttt agtatctaaa gggatcagca
      841 aagttgggca agagaatgta tacaggcttg ctttgaccaa ctagaaagag tcggggttat
      901 accagagttt cattctccac ttatggtaga tcgctaaaag attttcttca atatcaaata
      961 cctcccgtct atatattaaa acttttgatg tcgattaatt tacataatag acattttctg
     1021 ttataatatg cctagattga ataaagaggt ggtagactaa tgaatattac agatgtaagg
     1081 attaaaaaag tgacctcgca ggagggaaaa gttaaggctt ttgcatctat tacttttgat
     1141 gatgcatttg tagtgcatgg attaaaggtt atagatggta aaaatgggta ttttgttgct
     1201 atgccaagga gggatgttgg gaacggagag tttaaggaca ttgctcatcc tattaataat
     1261 cagatgagag agaatattca gagcgctgtc ctggaagcgt ttaataaatc tcaagactag
     1321 aaaaattggg gcgtggccaa gcggtaaggc atgggacttt ggatcccaga ttcgtaggtt
     1381 cgaatcctgc cgccccagcc aatatttatt aaggaggtaa aaatgattgc cggggtggtc
     1441 cttgctggcg gagtagggga gaggatgaat tcttctattc ccaagctttt tcataatctg
     1501 cttggaaagc ctatcataag gtatgtcctg gagaaccttt ctggattagc tctaaataaa
     1561 atcataattg tagtcaaatc ggatctgaaa gaaaaattcc agtatacatg tggggatgat
     1621 tttgtttatg tggttcagga taagccattg ggtagcggtg atgccctgaa aagagcaata
     1681 ccagaactgg aaaatttttc tactgtgctt gtggtaaatg gggatgcccc attaatcacc
     1741 acagaaattc tggagtcatt aatatcctcc cataatgctg ataatgtttc tgcaactgta
     1801 ttatctacgg ttattgatga ccccatgcat tacggcaggg tggtaaagga tgagctggga
     1861 aatgtaatca gaatagtcga ggctaaagat gcaactgaaa gagagaaaaa cataaaagaa
     1921 gttaatacgg gggcttattg tttctcggtg gatgacctga ggctggttat agacaggatg
     1981 actaacaata acgctcaggg ggaatactac ataacacaac ttatagaaat attgagcagt
     2041 atggggaaga gggttaattc gtatataaca gaccttggct ctcttgctct tgggataaat
     2101 tccagatttg atttatctct tgccatcgaa aaacttctga cggagaagat ggaagaactt
     2161 atgcagggag gagtgacaat tgtctctcca gagaatacat acatagagcc tgatgtaaag
     2221 gtaggaaggg atacagtaat agaaccattt tcagtgttaa aggggaacac agttataggt
     2281 gagaactgta ccataggtcc atcatcatat ctgctgaatg atgatatagg tgatagatgt
     2341 gaggtctttt attctgtagt taaaaattcc aagatagagt ctgatgtaag ggtaggtcca
     2401 tttgcccata tcagagatgg tgcgattata aagagcaatg ctgccatagg tgattttgta
     2461 gaggttaaaa ag
//
LOCUS       DAOTKU010000242         2457 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_34653_length_2457_cov_3.169858, whole genome shotgun sequence.
ACCESSION   DAOTKU010000242 DAOTKU010000000
VERSION     DAOTKU010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34653_length_2457_cov_3.169858"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="P5310_06060"
     CDS             <1..437
                     /locus_tag="P5310_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007505038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRU32958.1"
                     /translation="EEEWDEVLDTDLKGAFVCTRLIARHMIRNKDGRIVNIASVIGEV
                     GNIGQANYTAAKAGLIGFTKACAKELSRWGITVNAVAPGYIETEMTARLEGDIKNEYL
                     SKIPLGRFGRGEEVAKVVSFLVSKDASYITGQVINVDGGLVI"
     gene            450..686
                     /gene="acpP"
                     /locus_tag="P5310_06065"
     CDS             450..686
                     /gene="acpP"
                     /locus_tag="P5310_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HRU32959.1"
                     /translation="MAVLDKVKKIIAEQLGVDEEDITPDASFTDDLGADSLDLVELVM
                     AFEDGFDIEIPDEDAENIKTVADAVNYIQSKIEG"
     gene            683..1933
                     /gene="fabF"
                     /locus_tag="P5310_06070"
     CDS             683..1933
                     /gene="fabF"
                     /locus_tag="P5310_06070"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009708962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HRU32960.1"
                     /translation="MKKRIVITGIGLVTPYGVGKDVFWNGLTSGKNAIDYLSTVDTAN
                     LPVKIGGEVKDFDPESLLGKKEARRMDRFTQFAVIAGIEAIKDAGLDFDRVDRERVGV
                     IIGSGIGGIHILEQQHSLLLERGPDRISPFFIPMLISNMASAYLSIMFGLTGISFSVN
                     TACATGATAIGEAVKAIQSGECNIVITGGTDGAITPLSIAGFANMKALSTRNDPESAS
                     RPFDAQRDGFVMAEGAGILVIEDLETALSRGANIYAEIAGVGSTSDAYHLTAPEPEGR
                     LQARAMELALIYGGIEKEDVDYINAHGTSTPLNDAVETKAIKTLFGDLAYKIPVSSNK
                     SMIGHTLGAAGGIELIATALTIKEGIIPPTINYEYPDPECDLDYVPDIKREKEVSVAL
                     SNSFGFGGHNVSIALKKYYEAKVS"
     gene            1914..>2457
                     /gene="rnc"
                     /locus_tag="P5310_06075"
     CDS             1914..>2457
                     /gene="rnc"
                     /locus_tag="P5310_06075"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HRU32961.1"
                     /translation="MKLKYLEILEELDIHIPDELLNVVFTHRSFDEVNNNERLEFLGD
                     AVVGLFVAESLYKIDPPLSEGEMSKFKAKLVSRKHLANLALRLDLKDRMRVGGGEDLD
                     KGRERSTILGNALEALIGAIFLEEGFDKARIFLEKIFSIETFIEEDYKSKLQESIQSL
                     YKETPNYRVIGEEGPMHDRRF"
BASE COUNT          745 a          360 c          672 g          680 t
ORIGIN      
        1 gtgaagaaga atgggatgag gtactggata cagatttgaa gggggctttt gtatgtacca
       61 ggcttatagc cagacatatg ataagaaata aggacggaag gatagtgaat atagcgtcag
      121 ttattggaga ggtaggaaat attggacagg ctaattatac tgcagctaag gcagggttaa
      181 tcggctttac taaggcttgt gctaaagaac tcagccggtg gggtataacg gtgaatgcag
      241 tagctcccgg gtatatagaa accgagatga cggcgaggtt ggaaggggat ataaagaatg
      301 aatatttaag taaaattcca cttggaaggt ttggtagagg ggaagaagtg gctaaggtag
      361 tttcattttt ggtaagtaaa gatgccagtt atataactgg gcaggtaata aatgtcgatg
      421 gcggattagt gatataggag gtgcttactg tggcagttct tgataaggta aagaagatta
      481 tagcggagca gttaggtgta gatgaggagg atattactcc agatgcatct tttacagatg
      541 acctgggggc agactcatta gacctcgttg agctggtaat ggcttttgag gatggatttg
      601 atatagagat accagacgaa gatgcagaaa atataaagac tgtggctgat gctgtaaatt
      661 atattcagtc aaagatagag ggatgaagaa gcgtatcgtt attacaggta taggtttggt
      721 gactccttac ggggttggta aggatgtttt ttggaatgga ttgacctccg gaaagaatgc
      781 tattgattat ctttccactg tagatactgc gaatcttcca gttaagatag ggggagaggt
      841 taaggatttt gatcctgaaa gtcttttggg taagaaagaa gctcgaagga tggatcgatt
      901 tacacagttt gcagtgatag ccgggattga ggctattaaa gatgccggac ttgattttga
      961 cagggttgat agagagagag tgggggtcat tattggctcc gggatagggg gaattcacat
     1021 cctggagcag cagcattcct tgctcctgga aaggggacca gacaggataa gtcctttttt
     1081 tatccccatg cttatatcga atatggcaag tgcctatctc tctattatgt ttgggcttac
     1141 agggatctct ttctctgtta ataccgcctg tgctacagga gctactgcca taggagaggc
     1201 ggttaaggcg attcagtcag gtgaatgtaa tatagttata actggaggaa cagatggtgc
     1261 tattacacct ctatctatag ctggcttcgc aaatatgaaa gccctgtcaa ctagaaatga
     1321 tccggagagt gcgagcagac cctttgatgc tcagagggat ggttttgtaa tggcggaagg
     1381 agcaggtatc ctggtaattg aagatttgga aactgctctt tccaggggag ctaatatcta
     1441 tgccgaaata gcaggagtag ggagcacctc tgatgcttat cacctgacgg cacctgaacc
     1501 ggagggaagg ttacaggctc gagcaatgga actggctctg atatatggag gtatagagaa
     1561 ggaagatgtt gattatataa atgcccatgg cacatcaaca cctctgaatg atgctgttga
     1621 gacaaaggcg ataaagacgc tgtttggaga ccttgcatat aagatacctg taagttctaa
     1681 taaatctatg atcggacata ccctgggtgc agccggaggg atagagctta tagctactgc
     1741 tctgactatt aaagaaggta taattcctcc aactataaac tatgaatatc ctgatcctga
     1801 atgtgacctg gattatgtac cggatataaa gagagaaaaa gaggtaagtg tagcattgag
     1861 taactccttt ggatttggag ggcataacgt ttctattgcg cttaagaagt actatgaagc
     1921 taaagtatct tgaaatactt gaagagcttg atatacatat acctgacgaa cttttaaatg
     1981 tggtttttac tcatcggtct tttgatgagg tgaataataa tgagaggctc gagttcctcg
     2041 gagatgctgt tgtagggtta tttgttgctg agagtctata caagatagat cccccactat
     2101 ctgaaggaga gatgagtaag tttaaagcca aacttgttag caggaaacat ctagctaatc
     2161 ttgcattaag gcttgattta aaagatcgga tgagagttgg tggcggagaa gatttagata
     2221 aagggaggga gaggagtaca attttaggca atgccctgga agccttaata ggagctattt
     2281 ttttagaaga aggtttcgat aaggcgcgta tatttctgga aaagattttt agcatagaga
     2341 ccttcataga agaagattat aagagtaaac ttcaggagag tatacagtct ctttataaag
     2401 agactccaaa ttatagagta ataggagaag agggtcctat gcatgatcgc aggtttg
//
LOCUS       DAOTKU010000243         2450 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_34796_length_2450_cov_3.130689, whole genome shotgun sequence.
ACCESSION   DAOTKU010000243 DAOTKU010000000
VERSION     DAOTKU010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34796_length_2450_cov_3.130689"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="P5310_06080"
     CDS             complement(<1..192)
                     /locus_tag="P5310_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32962.1"
                     /translation="MIKKRALLFVSIIVITCVSVIVGGWLFLFYKSGGTIKDIAHNPS
                     RIVTFTQKMFYGPPAFNIVL"
     gene            complement(198..2267)
                     /gene="fusA"
                     /locus_tag="P5310_06085"
     CDS             complement(198..2267)
                     /gene="fusA"
                     /locus_tag="P5310_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HRU32963.1"
                     /translation="MKLDTGILRNVVLLSHGGVGKTSVTDAMLFDAKLVDRLGSVDAG
                     NSNVDSEPEEVKRKTTIMLKVFPIPWKDCKINIIDTPGYSDFIGDVLSGIKVAESAVL
                     LVCASSGVQVNTEILWNILEEQNKPRIIFINRLDREGANFLKVYTELREKLSSKIFPL
                     QIPLGEQVGFSGIINILDRKAYKFTNGTYQEIDIPAEYESQVEELREHLVEAVVENDD
                     ELLQKYLEGETLTGEEIVRGMKSAVKEGKAFPLLCGSAVKNVGIHQLLDGIKELLPSP
                     EDGKVEILNDDGSVTISNLDPSKPASAFIFKITADPFVGKLALFKVNSGILKSDVTLV
                     NITKDVEERIGHLAFPKGKTQEPVGEIITGDIGVIAKLQSVGTSDTLCDKSYMVKYTP
                     IEFPQPQYSVAVIPKSKGDEEKLGNALSRLQEEDPTFVSYYNAETHQTIVSGLGDLHV
                     EVILERLKRKFGVEVETDLPKIPYRETVTTKVKVQGRYKRQTGGRGQYGDCWIEIEPL
                     PRGGGYEFVDKIFGGAIPKNYIPSVEKGIKEAMEEGVLAGYPLVDVRVSLVDGSYHPV
                     DSSDMAFKIAGSMALKKGAAEANPVLLEPIMYIEVTVPEEFMGDCIADLNSKRARILG
                     MEPQGKYQIIKAHVPMAEILRYSIDLKSITQGRGRYKVEFSHYDVVPSKIAEEIIAAN
                     KKEKEEA"
BASE COUNT          651 a          584 c          399 g          816 t
ORIGIN      
        1 caggacaata ttaaaggcgg gaggaccgta aaacatcttc tgagtaaatg taactattct
       61 ggatggattg tgtgcgatat cctttatagt ccccccgctc ttatagaaga gaaataacca
      121 tcctccgact ataacactaa cacaggttat cactattata cttacaaaaa gtaatgctct
      181 cttttttatc ataaaggtca ggcttcttct ttttccttct tattagctgc tattatctct
      241 tccgcaatct tagatggaac cacatcataa tgtgagaact ccaccttata tctccctctt
      301 ccctgagtta tggatttaag atctatagaa taccttagaa tctcagccat agggacatga
      361 gcttttatga tctggtattt cccctgcggt tccattccta aaattcttgc ccttttactg
      421 ttaagatctg ctatacaatc acccataaac tcctcaggaa cggtaacctc aatgtacata
      481 attggttcaa gaaggaccgg attggcttca gctgcaccct ttttaagtgc catagagcct
      541 gctatcttaa aagccatatc agatgaatct accggatggt aagagccatc gacaagagac
      601 acacgtacat caactaatgg ataacctgcc aggacccctt cttccatggc ttcttttata
      661 cccttctcaa cggacgggat ataatttttg gggatggctc caccaaatat cttatctacg
      721 aattcatatc ctccacctct gggtagaggt tcaatttcta tccagcaatc accatactgt
      781 ccccgtccac cagtctgacg cttatatcta ccctggacct taaccttggt cgttacagtt
      841 tctctatatg ggatctttgg aaggtcagtt tcaacttcaa ctccaaactt cctctttagt
      901 ctctccagta taacctctac atgtaaatct cctaacccgg agactattgt ttgatgggtt
      961 tcagcattat agtaacttac aaatgttggg tcttcctcct gtaacctgct gagagcattg
     1021 cctaattttt cttcatcccc cttcgattta ggtattactg ctactgaata ctgtggttga
     1081 ggaaactcaa tgggagtata ctttaccata tagcttttat cgcataaggt atcagatgtt
     1141 cctacagact ggagttttgc tattacacct atatctccag ttattatctc acctaccggt
     1201 tcctgagttt ttcccttcgg aaaggcaaga tgtcctatcc gttcttctac atcctttgtt
     1261 atattcacta aagtcacatc ggattttagt attccggaat tgaccttaaa cagggctaat
     1321 ttcccgacaa atggatcggc ggtaatctta aatataaatg cagatgctgg cttggatggg
     1381 tccagattgg atatggttac agaaccgtca tcgtttaata tctctacctt gccatcttca
     1441 ggagagggca gtagttcttt aataccatcc aggagttgat gaatgcctac gtttttaact
     1501 gcagaaccac acaaaagagg gaaagctttc ccctctttta cagcagattt catacctctt
     1561 actatctctt cacctgttag tgtttctcct tctaaatact tctgtaagag ttcatcatca
     1621 ttttctacca ctgcttctac aaggtgctct ctcagctctt caacctgaga ttcatactct
     1681 gcaggtatat cgatctcctg atatgttcca tttgtgaact tgtaagcctt tctatctagg
     1741 atatttatta ttcctgaaaa acccacctgt tctccaagag gtatctgaag aggaaatatt
     1801 ttgctggaga gcttttccct cagttctgtg tagaccttta aaaagtttgc tccttccctg
     1861 tccagtctat ttataaatat tatcctcggt ttattctgct cttcaagtat gttccatagt
     1921 atttccgtgt tcacctgtac tcctgaagat gcacaaacaa gtaatactgc actttcagca
     1981 acctttatac ctgatagaac atctccgata aagtcagagt atccgggggt atctataata
     2041 tttatcttac aatccttcca ggggatgggg aagaccttca gcatgatggt agtcttcctc
     2101 tttacctctt ccggttcgga atctacatta ctgtttcctg catctacact tccaagtcta
     2161 tcaaccagtt tcgcatcaaa aagcatagca tcggtgacag aagtcttccc aacgccacca
     2221 tgtgatagta atacaacatt tctcagtatt cctgtatcta atttcatcta attccctcca
     2281 tatcttttta tattataaat aaaggggtat cctaattatt catccttttc ctttatcttt
     2341 gaaccagcca gatataaact ggaatccttc tctagaactc taagaagggc attaacaata
     2401 tctggatcta actgtcttcc gctgacattc agtaattctg cttttgcctc
//
LOCUS       DAOTKU010000244         2443 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_34897_length_2443_cov_3.218174, whole genome shotgun sequence.
ACCESSION   DAOTKU010000244 DAOTKU010000000
VERSION     DAOTKU010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34897_length_2443_cov_3.218174"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="P5310_06090"
     CDS             <1..613
                     /locus_tag="P5310_06090"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRU32964.1"
                     /translation="RRSPIPLYYQLKEILLDLIKTGELEGGRLPTEEELSTKYSVSRG
                     TVRKALAELESEGLIVTEAGRGTFVVKPKFEQSLLKFYSIGREMRERDEDFRTRVMIK
                     EITKPTRGIQEVLKIAEGDDLNRIVRVRYLENEPVIMESIFIPVRVCPELLEEDIESV
                     PIYDILEDKYNFKILKVRETLEPVILKREEAKVLESKVGDPAF"
     gene            829..2220
                     /locus_tag="P5310_06095"
     CDS             829..2220
                     /locus_tag="P5310_06095"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRU32965.1"
                     /translation="MNIFVIVTDSMRKDAIGVYGSNIKTPNIDSLGKDGAVFTNAFSE
                     GLPTLPTRTTWWTGRYTFPVRGWQPFEPSDLLLAEVLWDRGFTSCLVSDTYHMHKPVY
                     NCGRGFDTVVWVRGQEYDSWIVDDIPVDINKYHRLKGDETDDLWRPRFEQYLKNTSWY
                     KGEEDYCIARVFKEAIRWLEYITEKQKDKLFLWIDAFDPHEPWDPPEPYRSMYDPRYK
                     GQELIDPVPGLIEGYMTQEELNHTKSLYHGEVTFVDKWIGVFLEALKSLGLYEESIII
                     YTSDHGEPFGEHGIVRKARPWNYSELVSIPLIIKHPMLSMGKFIDSIVQTTDLMPTIL
                     DFLQIKKDTLVLNYLAPLSTGTFPQDMVSLSKNIIMEGHSLAPLMAGEVDKVRDYAFI
                     GHFKQSWTIRDLNYSFHLFLEDSRQSELYNLRVDPGEKNNMIDGEPEVAGELERRLKD
                     FASDIIEKRRKGL"
     gene            2217..>2443
                     /locus_tag="P5310_06100"
     CDS             2217..>2443
                     /locus_tag="P5310_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004214322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRU32966.1"
                     /translation="MRPNILMVVCHDLGQHLSCYENISVETSNIDSIAEEGLLFKNYF
                     ATAPLCSPSRGSIMTGRYPGTTGFMGLVNRG"
BASE COUNT          816 a          349 c          594 g          684 t
ORIGIN      
        1 taggcgaagt ccaatacctc tatattatca gctaaaggag attctacttg accttataaa
       61 gactggggaa ctggagggag ggagattacc cactgaagag gagctcagta cgaagtattc
      121 ggtaagtagg ggtacggtaa ggaaggctct tgctgaactt gaatctgagg gtcttatagt
      181 aacggaagca ggtagaggca catttgttgt taaacccaag tttgagcaga gtcttttaaa
      241 gttttatagt ataggcagag agatgagaga aagggatgaa gatttcagaa caagagtaat
      301 gattaaagag attacaaagc cgacaagggg tatacaggaa gttttaaaaa ttgcagaggg
      361 tgatgacctg aatagaatag taagggtaag atatctggag aatgagcctg taataatgga
      421 aagtatcttt ataccagtaa gggtatgtcc agaacttctg gaagaagata tagaaagtgt
      481 ccctatatat gatatcctgg aggacaaata caattttaaa attctaaaag taagggagac
      541 actagagccg gtaatactga aaagagagga ggcgaaggtc ttagagagta aggtaggaga
      601 tccagctttt taatagagag agttacatat ttaattgatg ataaaccgat agaggtaaga
      661 aagagcataa taaggggaga caggtttaag tttactactg agttgtatag gtaaaattga
      721 gagaaaggga ttagcattaa gagattttgt ttttagggat ggggtgtatt aagctcataa
      781 aaggttttaa agtttccaag agattacaat ttaatttgga ggttctggat gaatatattt
      841 gttattgtta cagatagtat gagaaaagat gccataggtg tatatggcag taatataaag
      901 accccaaata tagattctct gggtaaagat ggagctgtat tcacaaacgc ttttagtgaa
      961 ggactgccca cccttcctac aagaactacc tggtggacag ggagatacac ctttccggtt
     1021 agaggatggc aaccattcga accttcagat ctacttttag ctgaagttct ctgggataga
     1081 ggtttcacat cctgccttgt ttcagataca tatcatatgc acaaacctgt ttataactgt
     1141 gggagaggat tcgatacagt tgtatgggta agggggcagg aatatgactc ctggatagtt
     1201 gatgatatcc ctgtagatat aaataaatat catagattaa agggtgatga aacggatgat
     1261 ctatggagac ctagatttga gcaatattta aagaatacct catggtataa gggtgaggaa
     1321 gattactgta tagccagggt atttaaagag gctataagat ggcttgaata tataactgaa
     1381 aagcaaaagg ataaactgtt tctctggata gatgcctttg atcctcatga gccgtgggac
     1441 cctccggaac cttataggag tatgtatgat cccaggtata aaggtcagga gttaatagac
     1501 cctgtccctg gtctgataga aggatatatg actcaggaag aactcaacca tactaaatcc
     1561 ctttatcatg gtgaggtaac ttttgtggac aagtggatag gggtattttt agaggcgttg
     1621 aaatcattag gactgtatga agaatccata ataatatata catcagatca cggagaacct
     1681 tttggtgaac atggaatagt gagaaaggct agaccgtgga actatagcga gcttgttagc
     1741 atacctctga ttataaaaca tcctatgtta tcaatgggta agtttataga tagtatagtt
     1801 caaactaccg atctgatgcc taccatcctg gattttctgc agatcaaaaa ggatacatta
     1861 gtacttaact atctggctcc actctctacc ggcacgtttc cacaggatat ggtttctctc
     1921 tctaagaata taataatgga gggacacagt ttagctcctt tgatggcagg agaggtggat
     1981 aaagttagag actatgcttt tattggtcat ttcaagcagt cctggacaat aagggactta
     2041 aattatagtt ttcatctttt tttagaagac tccagacaat cagaactcta taatttaagg
     2101 gtcgaccctg gcgagaagaa taatatgatt gatggagaac cagaggtagc tggagaatta
     2161 gagagaagat taaaagattt tgccagtgat atcatagaaa agagaaggaa aggtttatga
     2221 gacccaatat attgatggtc gtatgccatg atctgggtca acatcttagt tgttatgaga
     2281 atatctcagt agaaacttca aatatagact ctatagctga ggaaggtctt ttatttaaaa
     2341 actattttgc taccgcacct ctatgtagtc caagtagggg gagtataatg acggggagat
     2401 atcctggaac caccgggttt atgggacttg taaatagagg atg
//
LOCUS       DAOTKU010000245         2409 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_35517_length_2409_cov_4.220051, whole genome shotgun sequence.
ACCESSION   DAOTKU010000245 DAOTKU010000000
VERSION     DAOTKU010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35517_length_2409_cov_4.220051"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..117)
                     /locus_tag="P5310_06105"
     CDS             complement(<1..117)
                     /locus_tag="P5310_06105"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HRU32967.1"
                     /translation="MLITGSSGQLGKAVVEVFFKDGMDVYPVPRSRVDITDRN"
     gene            complement(120..1163)
                     /locus_tag="P5310_06110"
     CDS             complement(120..1163)
                     /locus_tag="P5310_06110"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HRU32968.1"
                     /translation="MKAFIMAAGEGTRLRPLTYIRPKPLVPLGNKPIISRIISLLKSG
                     GITDIIINLHYKGERIKEFIGKGEDLGVDIVYSEEEVLLGTAGGVKKMEEFLPDTFVV
                     ISGDIATDINIKEMVAFHKKKKALFTLAVSIVDDPTKYGVVSLNSDGKIQKFLEKPSR
                     DRVFSPLVNAGIYIMEKEVLKDVPKDTFYDFGKQLLPILLERNAPLYGYLSSAYWKDI
                     GNITDYKKVHSDIGDGKLQIPHHGELFTDNVRIGIGTYVDKANFFGNVFIGNNVYIED
                     GVTIVGPTFIGDNAKIEKGARIEESIIWEDVCIEEYSTVVKSVIGDNTRILKESYLVD
                     GVFCRIPEGAIDS"
     gene            complement(1166..2254)
                     /locus_tag="P5310_06115"
     CDS             complement(1166..2254)
                     /locus_tag="P5310_06115"
                     /inference="COORDINATES: protein motif:HMM:NF013486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HRU32969.1"
                     /translation="MREIDIKEERIRKLLEENKSDGLLITLKYNFAWATGGKYNHVGL
                     LTEVGATSLLITNSSSYIIASSIEAPRIEKEEVGALGHYKLEVFPWYEPDEKLRIVEK
                     ITGGNFITDTPFGNARVVNLLPLRYPLTEDEVIRIKALGKSASLCMEDVCKSIMPGYT
                     ELEIAGMMSESLFSNGIYPSVLLVAGDERIFNFRHPIPTDNKVSRYAMVVICGMKDGL
                     QVAVTRLVHFGRIDSDIRKKHNAVVRIDSILIHNTMVGNRWSDILAKGIKAYEDLGYP
                     DEWKKHHQGGPTGYQSREFTVNPKTQGVVSKWQPVAWNPSITGTKSEDTILTGEESPE
                     VITPTFNWPVVDVELDGMIYKRPDILEK"
     gene            complement(2266..>2409)
                     /locus_tag="P5310_06120"
     CDS             complement(2266..>2409)
                     /locus_tag="P5310_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003540746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like YkuK family protein"
                     /protein_id="HRU32970.1"
                     /translation="IHVDIGMKGETRQLIKEVVGMITSSGYSAKIKPESFGASKVADK
                     HSK"
BASE COUNT          612 a          593 c          390 g          814 t
ORIGIN      
        1 atttctgtcg gtaatatcaa ccctggaacg cggtacagga tacacatcca taccatcctt
       61 aaagaatacc tctaccactg ctttccccaa ctgtccagaa gaaccggtta tcagcacttt
      121 caactgtcta ttgctccttc aggtatcctg caaaagactc catcgaccag ataactctct
      181 tttaatatcc ttgtattatc acctataacc gacttaacta cagtcgaata ttcttctatg
      241 cagacatcct cccatattat tgattcctct atccttgccc ctttttctat ctttgcatta
      301 tcaccgataa atgttggacc aactattgtt actccatctt cgatatacac attgtttcct
      361 ataaatacat tgccaaagaa gttcgcctta tcaacatatg tcccgatacc tatccttaca
      421 ttgtcggtga agagttcccc gtgatgtgga atctgaagtt ttccatcacc tatatcactg
      481 tgtacctttt tataatcagt tatatttcca atatccttcc agtaagcgct ggagagatat
      541 ccatagaggg gggcattcct ttccagaagg atggggagga gctgtttacc aaaatcgtag
      601 aatgtatctt ttgggacatc tttaaggact tccttttcca taatatatat cccggcattt
      661 acaagtggac tgaagaccct atcccttgat ggtttttcca aaaatttctg tatcttgcca
      721 tcactattta aagagacgac cccatatttt gtcgggtcat ctactattga taccgccagt
      781 gtaaagagag cctttttctt tttgtggaat gcaaccattt cttttatgtt tatatcggtg
      841 gctatatccc cacttatgac cacaaaagta tccgggagaa attcctccat cttcttaact
      901 ccaccggcgg tgccaagaag gacctcttct tcagaataaa cgatatctac tccaaggtct
      961 tctcctttac cgatgaattc ctttatcctt tctcctttgt aatggagatt tattatgata
     1021 tcagtaattc caccactttt gagcagagat attatcctgg atattatcgg tttattccct
     1081 aagggaacca gaggtttcgg tcttatatat gtaagaggac gtagtcttgt cccctctccg
     1141 gcagccataa taaaagcctt catctttatt tctccagaat atcgggtctt ttatatatca
     1201 taccgtctag ctctacatcc actacaggcc aattaaatgt cggggttatt acctcaggtg
     1261 attcttcgcc agttaaaatt gtatcttcag actttgttcc tgttatagag gggttccatg
     1321 ctactggttg ccacttactg acaactccct gagtctttgg atttacagtg aattctctgc
     1381 tctgatatcc agtaggtcca ccctgatgat gtttcttcca ctcatccgga taacccaggt
     1441 cttcgtaagc ctttatacct tttgccagga tatcgctcca cctgttccct accatcgtat
     1501 tatgtataag gatagaatcg attctcacca cagcattatg ttttttcctt atatcactat
     1561 ctatcctgcc aaagtgtacc agtcttgtga cagctacctg aagaccgtcc ttcatcccgc
     1621 atatcaccac catggcgtat cgggatactt tattatctgt aggtatagga tgcctgaaat
     1681 taaagatcct ttcatctcca gctaccagca gtacacttgg atatatccca ttggagaata
     1741 gactttcaga catcataccg gcgatctcca gttcagtata acctggcatt atagacttac
     1801 agacatcttc catacataaa ctggcagatt taccaagagc ctttatcctt atgacctcgt
     1861 cttcagttaa cgggtatctc aggggcaata agtttaccac cctggcattt ccaaatgggg
     1921 tatcggtgat gaaatttcct ccagtaatct tttctactat tctcaatttc tcatctggct
     1981 cataccaggg gaagacctct aatttataat gtcctaaggc tccgacctcc tctttttcta
     2041 ttctgggagc ctcaatagag ctggctatta tatatgaaga tgagtttgtt atgagtagac
     2101 tcgtcgctcc cacctctgtt aagagtccta catgattgta cttccctccg gttgcccagg
     2161 cgaagttata ctttaatgtt atcagcaatc catcagactt attttcttca aggagctttc
     2221 tgatgcgttc ttcctttata tctatctctc tcatcttttt tcctcctatt tagaatgttt
     2281 atctgccact ttacttgcac caaaagattc tggttttatc ttggcagaat aaccactgga
     2341 agtaatcatc ccaaccactt cttttataag ctgtcttgtc tctcccttca ttcctatgtc
     2401 gacatgaat
//
LOCUS       DAOTKU010000246         2397 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_35780_length_2397_cov_3.271990, whole genome shotgun sequence.
ACCESSION   DAOTKU010000246 DAOTKU010000000
VERSION     DAOTKU010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35780_length_2397_cov_3.271990"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="P5310_06125"
     CDS             complement(<1..237)
                     /locus_tag="P5310_06125"
                     /inference="COORDINATES: protein motif:HMM:NF013878.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HRU32971.1"
                     /translation="MGRSKRKSFSITLILSIIFAVICIIAVWNVKVGDHEKGLQPDYV
                     EPETETMPYESIKPPTEEKTDKIIIASDPDREGEA"
     gene            complement(224..1375)
                     /locus_tag="P5310_06130"
     CDS             complement(224..1375)
                     /locus_tag="P5310_06130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRU32972.1"
                     /translation="MRRNSIVYSLIFLLAISLAFLAGMNYRGELFAQNSIDRNLQVLS
                     QAYYFITNNYVEKVDGKNVSYKGIEGMVSSLNDPYSYFMRPEDYKAMGEEFQGSFEGI
                     GVEIGMNENKQIVVISPIDDTPAQKAGLKTGDIIIKVEDKSTEGMNLDQVSSLIRGPA
                     GTKVRLTIQREGEKNPVEITVTRAVIRLLTVSSKLLDDNIGYIKIKAFREPTTSELNN
                     ALDQILPKAKKGIILDLRNNPGGLLSSAIEVASFFIQKGTVLYAEDRNGNREQYDIIP
                     SLYRVKVPMVVLVNEGSASASEIVAGALQDYKRATVVGTKTFGKGLVQSVVQLADGSA
                     ISLTVQRWLTPKLRYIHKDGIKPDIQLDWDQKGTDIQLERAKEVLLGKK"
     gene            complement(1409..>2397)
                     /locus_tag="P5310_06135"
     CDS             complement(1409..>2397)
                     /locus_tag="P5310_06135"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRU32973.1"
                     /translation="LEQAKEKEHNLVKEINTLDQEIEIISERISSLEKHLNQVLVLKK
                     EAERRLMIAQNNLKSSRERLGEKVFFIYRYGNVSYLELLLGASDPYNFLTRYRFLSKL
                     AEEDAILIKSVQEEMENVKKWKIELNNKEKDIKDTRNELLKQKDSLTKKKEERNRLLA
                     KTSSERAEYEKALRELEESSRALETLIKRLQQTAKGPGLVGNLFWPTNNRLITSQFGH
                     RRHPIWGIDMFHSGIDIAGNYGDNIYAVSDGRVIYSGWQSGYGKVLIIDHGNGMSTLY
                     GHCSQLLVNEGATVKKGQIIGKVGSTGWSTGPHLHFEVRKNGSPINPLSMIK"
BASE COUNT          607 a          562 c          370 g          858 t
ORIGIN      
        1 ggcttcccct tctcggtccg ggtcggaagc gataataatt ttatctgttt tttcttcggt
       61 tggcggtttt atactttcat atggcatagt ttctgtttct ggttcaacgt aatcaggttg
      121 gagacctttt tcatggtctc caacctttac attccatact gctataatac agatgacggc
      181 aaatatgatg gataaaatta aggttatact aaaagatttc ctcttacttc ttcccaagta
      241 atacttcttt tgccctttct aactgaatgt ctgtaccttt ctggtcccag tctaactgta
      301 tatcaggttt tataccatct ttatgtatat atcttaattt aggggtaagc catctctgaa
      361 ccgttaaact gatagcagat ccgtcggcta attgaacaac actctgtacc agtcccttac
      421 caaatgtctt ggttccaaca actgtagctc ttttataatc ctgtaaagcg ccggcaacaa
      481 tctcagatgc gcttgcacta ccctcattta ccaggacaac catcggaacc tttacccggt
      541 aaagactcgg tattatatca tactgttctc tgttaccgtt cctgtcttct gcataaagta
      601 cagttccctt ctgtataaag aaacttgcca cttcaatcgc agaactgaga agtccccctg
      661 gattattcct taaatccaga ataattccct tcttagcttt ggggagtatc tggtccaggg
      721 cattgtttaa ttcacttgta gtcggttctc tgaatgcctt tattttaata taacctatat
      781 tatcatctaa taacttactg gagacggtta aaagtctgat caccgctctg gtaactgtga
      841 tttctacagg atttttctct ccctctctct gtattgtaag tctcaccttg gttccagctg
      901 gtccacgtat gagactactt acctgatcca gattcatacc ttcagtagat ttatcctcga
      961 ccttgatgat tatatctcct gtctttaaac cagccttttg agccggggta tcatctatag
     1021 gggaaataac tactatctgt ttattctcgt tcatacctat ctctactcca atcccttcaa
     1081 aactcccctg aaactcctct cccatagcct tatagtcttc tggtctcata aagtatgaat
     1141 atgggtcatt gagactggag accatccctt caattccctt ataggataca tttttcccat
     1201 ccaccttttc cacatagtta tttgtaataa agtagtaggc ttgcgatagg acctgaagat
     1261 tcctgtctat actattttgg gcaaagagct cccctctata gttcattcca gccaggaaag
     1321 ccaggcttat tgctaataaa aatattaaac tgtaaactat agaattacgt ctcataattg
     1381 atcctccaat cttttagtct tccagatatt attttatcat agataatgga tttataggtg
     1441 atccattctt tcttacctcg aaatgtagat gaggtcctgt agaccatccg gttgaaccca
     1501 ccttacctat aatctgcccc ttcttaaccg ttgccccttc atttaccagt aactgagaac
     1561 aatgtccgta taatgttgac ataccattcc catggtcgat aataagaacc tttccatatc
     1621 cgctctgcca accagagtat atcacccttc cgtcacttac agcatatatg ttatccccat
     1681 agttaccagc aatatctatt ccagaatgga acatatctat cccccatata ggatgtcttc
     1741 tatgaccaaa ttgtgatgtt ataagtctat tattggtagg ccagaagaga ttaccaacta
     1801 gtccaggacc tttagcggtc tgttgtagac gttttatcag ggtttccagt gccctggaac
     1861 tctcttctaa ttcccttaga gccttttcat attcagccct ttcgcttgaa gttttagcca
     1921 agagcctgtt tctctcttct tttttcttgg tcagagaatc tttttgtttt agcagttcat
     1981 ttctggtatc ttttatatct ttttctttgt tatttaattc tatcttccac ttcttgacat
     2041 tctccatctc ttcctgaaca gattttatta atattgcatc ttcttcagct aattttgata
     2101 gaaatctata ccttgtcaga aagttgtatg ggtcgctggc acctaaaagt aactccagat
     2161 aacttacatt accatacctg tatatgaaga agaccttctc accgagtctt tccctggaag
     2221 actttagatt gttctgagct atcataagcc ttctttctgc ctctttttta agaaccaata
     2281 cctgatttaa gtgtttttcc aaactactga tcctctcaga tattatttct atctcctgat
     2341 ccagtgtatt tatctctttt acaagattat gctctttctc cttagcttgt tcgagga
//
LOCUS       DAOTKU010000247         2396 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_35799_length_2396_cov_3.013669, whole genome shotgun sequence.
ACCESSION   DAOTKU010000247 DAOTKU010000000
VERSION     DAOTKU010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35799_length_2396_cov_3.013669"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(142..444)
                     /locus_tag="P5310_06140"
     CDS             complement(142..444)
                     /locus_tag="P5310_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32974.1"
                     /translation="MWYKLSGFGDEISPDFDTQLKVLKEEGIDYLELRSAFGKNVVNL
                     DDEDIDAIQKFLDKYGIKISAIASPIGKVRAREDIEGQKEKLSNAINWLISLTLHI"
     gene            complement(457..1254)
                     /locus_tag="P5310_06145"
     CDS             complement(457..1254)
                     /locus_tag="P5310_06145"
                     /inference="COORDINATES: protein motif:HMM:NF016826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HRU32975.1"
                     /translation="MESKLTRVPEGKGVLEVPVQGLEWIRLYVATLEKGERLSLGEKE
                     RELGCLLTTGEIEVNGKYVLGPRREIFNSKPWGIYVPPGKSVDIKVLEDSELVFATAK
                     SEPDGADVVVISPEEIDRRVVGVHNWKRYVTQVIDNKVGARHIIMGETVNPPGNWSSY
                     PPHKHDKHNPPYEWKMEEVYLYRLNPPGGFGLQRIYTEEGDVDVVYPVTNNTVLPITR
                     GYHPVVAAPGYSLYYLWVLGGEDPVNIPRDDTRHAWVKWTEQIVREL"
     gene            complement(1254..2009)
                     /locus_tag="P5310_06150"
     CDS             complement(1254..2009)
                     /locus_tag="P5310_06150"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HRU32976.1"
                     /translation="MMKFGCCGGIEEIELIASGGAEFIEFTVTGIMQSEIEKIKKELD
                     RVNLRAYSFNVFLPGDLHITGPDVDLKKIDEYVKEALRRVSFLGGEIIVFGSGRSRSF
                     PEGFSRDAAREQIIEFLKIVDKYAREFNIKIAIEPLNRKESNIINTTLEALEFARAID
                     SPNIGVLIDSYHADLEGEPLSNIEKIKDKLYHVHVSDRGRVAPGKNNYDFKSLFNTLK
                     AAGYNGCISIECRWEDKAKELPESLEYLRKEWE"
     gene            complement(2009..>2396)
                     /locus_tag="P5310_06155"
     CDS             complement(2009..>2396)
                     /locus_tag="P5310_06155"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRU32977.1"
                     /translation="LKEVGVDYNPDLISFNVPVSESGYKEAIKLLCTGEAPTAIFTYN
                     DVMAFGVIKAAKELGIKIPEELSVVGFDDIFFSSFTDPPLTTIKQFKEELGRMAVELL
                     FKLMEGERESLLIEPELIIRNTTSRR"
BASE COUNT          592 a          624 c          382 g          798 t
ORIGIN      
        1 catatatcct cttctcattc tcatggagga ggactgcccc ttccttttca gcaatcttta
       61 ccagttctcc aagtctttcc agtgagatct ttctcagctc ttctatgccc ataccttctt
      121 caggatagaa tgagaagatt cttatatatg gagtgttaaa gatatgagcc agtttatagc
      181 gttgctgagt ttctctttct gcccctcaat gtcttctctt gcccttacct tacctatagg
      241 ggaagctatt gcagatatct tgattccgta cttatccaga aacttctgga ttgcatcaat
      301 atcttcatcg tctaaattaa ccacattttt cccgaatgca cttctcaact ccagataatc
      361 tatcccctct tctttcaaga ctttcaattg ggtatcaaaa tctggtgata tttcatcgcc
      421 aaatccagaa agcttatacc acatttttcc cctccctcat aactctctca ctatctgttc
      481 agtccacttt acccaggcat gtctggtgtc atctcttggt atatttacag ggtcctctcc
      541 ccccagtacc cagagatagt acagtgaata tcccggtgcg gctaccacgg gatggtatcc
      601 tcttgttatg ggcagtactg tgttgtttgt tacaggatat accacatcaa catctccctc
      661 ttcggtatat atcctctgaa gaccaaatcc gccaggagga ttcaatctgt acagatatac
      721 ctcttccatc ttccattcgt aaggaggatt atgtttgtcg tgtttatgtg gtggatagct
      781 ggaccagttc ccaggtggat tcaccgtctc tcccattatt atatgcctgg caccaacttt
      841 gttatcgatt acctgggtca cgtatctttt ccagttatgg acgccaacca cccttctatc
      901 tatctcttca ggggagataa ctaccacatc tgccccatca ggctctgatt ttgccgtagc
      961 aaacacgagc tcactatctt ccagaacctt tatatctaca gatttgccgg gtggtacata
     1021 tataccccac ggtttactgt taaagatctc tcgccttggt cccagtacat acttaccatt
     1081 gacctctatc tcaccagtgg tcaatagaca tcccagttct ctctcttttt ctcccagaga
     1141 gagacgttcc cctttctcca atgtagccac atataatctt atccactcca acccctgaac
     1201 agggacttcc agtactcctt taccttccgg tactctggta agtttgcttt ccattattcc
     1261 cactcctttc tcagatattc taagctctcc ggtaactctt tagccttgtc ttcccatctg
     1321 cattcaatag atatacatcc attatatcct gctgctttaa gagtattgaa aagagatttg
     1381 aaatcatagt tattctttcc aggtgcaacc cttcccctgt ctgacacatg aacatgatag
     1441 agcttatcct ttatcttttc tatattagat agaggttctc cttcaagatc tgcatggtag
     1501 ctgtcaatca ggacacctat atttggactg tcaatggctc tggcaaattc taatgcctcc
     1561 agtgtggtgt ttataatatt actttccttt ctgttaaggg gctctattgc tatctttata
     1621 ttaaattctc ttgcatattt atctactatc ttcaaaaatt ctatgatctg ctctcgagct
     1681 gcatctcttg agaatccttc agggaaagac ctggaccttc cactaccaaa aactataatc
     1741 tctccaccaa gaaaacttac ccttctcaat gcctctttta catattcatc aatttttttc
     1801 agatccacat ctggaccggt gatatgcagg tctccaggca aaaagacatt gaaggaataa
     1861 gccctgagat tcaccctgtc aagctctttc ttaattttct ctatttcaga ctgcatgata
     1921 ccggttactg taaactctat aaattccgct ccaccagatg ctatgagttc aatctcttct
     1981 ataccaccac aacatccaaa tttcatcatt acctccttga ggtcgtattt cttatgataa
     2041 gttctggttc aatcagaaga ctctccctct ctccttccat caatttaaaa aggagctcca
     2101 ccgccattct tcccagctct tctttgaact gttttatggt ggtcaggggt gggtcagtaa
     2161 aactggagaa aaatatatcg tcaaatccca caactgaaag ttcttcaggt atttttatcc
     2221 cgagttcctt ggcagccttg ataactccaa atgccataac gtcattgtag gtgaatatgg
     2281 cggtaggtgc ctctccggtg cacaataact ttatagcctc tttataccct gattcggata
     2341 ccggtacatt gaaagatatc aggtcagggt tataatctac ccctacctct ttcagt
//
LOCUS       DAOTKU010000248         2394 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_35826_length_2394_cov_4.400171, whole genome shotgun sequence.
ACCESSION   DAOTKU010000248 DAOTKU010000000
VERSION     DAOTKU010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35826_length_2394_cov_4.400171"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /gene="purH"
                     /locus_tag="P5310_06160"
     CDS             <1..501
                     /gene="purH"
                     /locus_tag="P5310_06160"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013290408.1"
                     /note="involved in de novo purine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HRU32978.1"
                     /translation="KKNLRILVHSNFYQLPEGLEIKGIQGGFLIQEWDTGDVKEFKVV
                     TETPLEETLKDDLIFAYKVVKFVKSNAIVVAKDGKTLGIGGGQPNRVDSVRLAISHAG
                     KDSSGAVLASDGFFPFPDSVEEAGKAGIKAIIQPGGSIRDKESIEMANKYRVAMVFTG
                     MRHFRH"
     gene            658..861
                     /locus_tag="P5310_06165"
     CDS             658..861
                     /locus_tag="P5310_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HRU32979.1"
                     /translation="MVTGKVKWFNAQKGYGFITRDDGGDVFVHYSAIKGQGYRTLEEG
                     QKVEFEVVQGEKGPQAANVIVLR"
     gene            complement(904..1362)
                     /locus_tag="P5310_06170"
     CDS             complement(904..1362)
                     /locus_tag="P5310_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015312359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HRU32980.1"
                     /translation="MDKEYIRFLSRAGLYAAIYAVLTVVLAPISYGIMQIRVAEALTV
                     LPLLDRAAIPGLFIGCLIANVVGGVGFIDIGLGSIFTLIAAYLTSKVKKDWQAPIPPV
                     IINALGVGTYLSILLKTPVYWGIFWVGLGESIACFGLGLPLLYFIKRIKK"
     gene            complement(1355..2227)
                     /locus_tag="P5310_06175"
     CDS             complement(1355..2227)
                     /locus_tag="P5310_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012002323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4438 domain-containing protein"
                     /protein_id="HRU32981.1"
                     /translation="MRLNLERLVKLSVWGDISSPTIVSPYRVNSSGKGLVLPTLGGIT
                     YNIRVGDPALGWAGDHVEPGVSLRNKDRDESNALNILSCIGNRARVISGDAKGEIGIV
                     TGKHGGIEHVLIDFPERVLERLNIGDKIQIESYGQGMEILNFPEIKVMNISPDLFSLL
                     NIKEDNNGLKIGVRKVIPAELMGSGIGSASSFSGDYDIMTQDRETLRELGLEDLKLGD
                     IVGIKDMDDRYGRTYKKGARSVGVVIHCDCVQSGHGPGVTVVLSSSDNSLEFFEDGKA
                     NIAHILKIGRYGDG"
BASE COUNT          696 a          518 c          469 g          711 t
ORIGIN      
        1 aagaaaaatc tgcggatact tgttcatagt aatttctatc agctacctga aggactggag
       61 ataaagggaa tacagggcgg atttctcatt caggaatggg ataccgggga tgtaaaagaa
      121 tttaaagtgg tgacggagac tccattggag gaaactctta aggatgacct gatcttcgcc
      181 tataaggttg taaaatttgt gaagtccaat gccatagttg ttgctaagga tgggaaaacc
      241 cttggtatag gtggtggaca acctaacagg gtggattcag tgagacttgc tatctctcat
      301 gcaggaaaag attcttccgg ggcggttctt gcgtcagatg gattcttccc gtttccggat
      361 tctgtagaag aggctggtaa agcagggata aaagctatta ttcagccagg tggctctata
      421 agagacaagg aatccataga gatggcaaat aaatataggg tggcgatggt atttaccggt
      481 atgagacact tcagacatta aaaaataata tagattataa attttcgggt gtcattacat
      541 ttttccttag atgagggata tttaagaaaa tcgtgactgt tgacagggat atttgggagt
      601 agtataatat cggttggata cttagtccat ttttaaactt tttaggaggt atttgtaatg
      661 gtaacaggta aagtgaagtg gtttaacgcc cagaaagggt acggatttat cacccgagat
      721 gacggtggtg atgtatttgt tcactactcc gccattaaag gtcagggtta tcgaactcta
      781 gaagaagggc aaaaggtaga gtttgaagtt gtccagggag aaaaaggacc acaggcagct
      841 aatgtaattg tattgaggta acttaacctg gaaaagaaca ggctctggag agaatccaga
      901 gccttatttt tttattcttt taataaagta gagaagagga agtcctaatc cgaagcaagc
      961 tatcgattct cccaatccga cccagaagat accccagtat acaggagttt tcagaagaat
     1021 agagagatat gtgcctaccc ccagtgcatt aattataact ggtggaatag gagcctgcca
     1081 gtcttttttt actttagatg taagataagc agctataagg gtaaatatac tgcccaatcc
     1141 gatatcgata aatcctaccc ctcctactac attggctatt aaacaaccta taaataaacc
     1201 gggaattgcc gccctatcaa gtaggggaag gactgtcaat gcctcagcca cccttatctg
     1261 cattatccca tagcttattg gtgcaagaac tactgtaagt acggcataaa tagcggcata
     1321 taatcctgct ctggatagaa atcttatata ctccttatcc atcaccgtat ctgcctattt
     1381 tgagaatatg ggcaatattt gcctttccat cctcaaagaa ttccagtgaa ttgtcagaac
     1441 ttgagagtac taccgtaacc cctggtccat gccccgactg aacacagtca cagtgtataa
     1501 cgactccaac actccttgcc cctttcttat aagtcctgcc atatctatca tccatatctt
     1561 ttattcctac tatatccccc aactttaaat cttcaagtcc aagttctctt aatgtctctc
     1621 tatcctgagt cataatatca taatcacctg agaaactgct ggcagaacct atgccggaac
     1681 ccataagttc tgctggaata accttcctta cacctatttt taatccatta ttatcttctt
     1741 ttatattgag aagactgaac aggtcgggag agatattcat aacttttatc tccgggaaat
     1801 tcagtatctc catcccctgc ccgtaactct ctatctgaat cttatcccca atattaagtc
     1861 tttccagtac tctttcaggg aaatctatca aaacgtgctc aataccacca tgtttccctg
     1921 tgactatacc aatctctccc ttagcatcac cacttataac tctggctcta tttccaatac
     1981 aggagagtat attcagcgca ttactctcat ctctatcctt attccttaag cttactccag
     2041 gttccacatg gtctcctgcc catcccagtg cggggtcacc aactcttata ttgtatgtaa
     2101 tccctcctaa tgttggtaat actaaccctt taccacttga gttaactcga tatggtgaga
     2161 ctatcgttgg agaagagata tctccccata ctgataactt tacaagtctc tctagattaa
     2221 gcctcatggt cccgtctcct ttctacccgg gggtggggta ttgcttatac gtcccccgaa
     2281 ttcttatctg ttcagattat tcctttgcct tctgatttgc catataagca cagatagcga
     2341 taactgctcc gactataata tgtagccatg cagaaaatcc tcttacacct gcca
//
LOCUS       DAOTKU010000249         2381 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_36100_length_2381_cov_3.729579, whole genome shotgun sequence.
ACCESSION   DAOTKU010000249 DAOTKU010000000
VERSION     DAOTKU010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36100_length_2381_cov_3.729579"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..989
                     /locus_tag="P5310_06180"
     CDS             <1..989
                     /locus_tag="P5310_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32982.1"
                     /translation="KLLNLFFVLIFTVAITSQLLAADKTPSDILTTAWNIDGKINFIA
                     TETTGMYVRDKFNVTISRVFSLSENKFRREYIFPPFLVGDLLIDDGTRSYYYQSQKKV
                     LTISPSIFGPSQRESHNKLLKLILRNYNLVSSSDKYLGRDVSRISVYPADGRFPLLIL
                     WIDNQTNVILKREKYLPDGKIYEYSFYTDIDFNVIPNERLFVWIKPKEEFRVIEHKEK
                     FIPLEEAGKYGPEIPYSFQKKGYECIGIKEVPGGIACQFSDGIKNFMVFNLESLPPIP
                     PSSKKITTDGIRYSYWQIDSINGFAWNYKKESFLVIGMVERDIIEELIKSIR"
     gene            1002..2069
                     /locus_tag="P5310_06185"
     CDS             1002..2069
                     /locus_tag="P5310_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012900411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRU32983.1"
                     /translation="MRRIIALTAILAFLGGLILGYVGNRENSINSALAQTEFLSNEEA
                     IYVSIAEKLGPSVVNISTVSIVQYFFQPIPAEGAGSGVIISRDGYIITNNHVVEGAKS
                     IKVTLEDGQTLEGKLIGRDPFTDIAVIKVNMTDLPYAKFSDPSRLKVGQIAIAIGNPF
                     GLGKTVTAGIVSALERSIQPDQGVIIESLIQTDAAINPGNSGGALVGSNGEIIGINTA
                     IYQGAQGIGFAIPSNVAKKIAEDIIEKGYASHPWLGIKGETLNRRTALYYGLPIDYGV
                     IIGEVVKSSPADRAGLKVGDVIVGVDNERIDSWNTLLLKILKTKNNTVTLQIMRKSSK
                     LELKVQIDERPKQFATNGGYL"
     gene            2066..>2381
                     /locus_tag="P5310_06190"
     CDS             2066..>2381
                     /locus_tag="P5310_06190"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRU32984.1"
                     /translation="MIKSLRILLIIFLLIPGLALAGEVWNLETALKNIDNAPELKVLK
                     AQLESADSQLKQAQALFLPKLNGTVGYTRGESAVNNGTIQVDQPSATLTLTYNLSENS
                     PAG"
BASE COUNT          813 a          385 c          540 g          643 t
ORIGIN      
        1 agaaattact caatttattt tttgtattaa tctttactgt ggctataaca tcccaattat
       61 tagcagctga taagacccct tcagatatat taactactgc atggaacatt gatggtaaga
      121 taaactttat agcaactgaa actacaggaa tgtatgtccg agataaattt aatgttacca
      181 taagtagagt attctctctc tcagagaata agtttagaag agagtatata ttccctccat
      241 ttttagtggg ggaccttcta atagacgatg gcaccagaag ctactactat caatcacaga
      301 agaaggtgct aaccataagt ccttctatat ttggaccctc tcagagggaa tctcataata
      361 agcttctaaa acttatacta agaaattata accttgttag ctcttcagat aaatatctag
      421 gtagagatgt gtccaggata tctgtttatc cggcagatgg caggtttcca ctacttatcc
      481 tctggataga taatcagacc aatgtaatac ttaagagaga aaaatatctc cctgatggga
      541 aaatatacga gtatagtttt tatacagaca tagactttaa cgttattccc aatgaaaggt
      601 tatttgtatg gataaaacca aaggaagagt tcagggtaat agaacataaa gaaaaattca
      661 tacctttaga ggaagctgga aagtatggtc cagagatccc ttactctttt caaaagaaag
      721 gatatgagtg tataggtata aaagaggttc ccggaggtat agcctgtcaa ttctccgatg
      781 ggattaaaaa ttttatggtc ttcaatcttg agagtctacc acccatccca ccctcttcta
      841 aaaagattac aacagatggt ataaggtatt cctactggca gattgatagc ataaacgggt
      901 tcgcctggaa ttataaaaaa gagagctttc tagtgatagg aatggtagaa agagatataa
      961 tagaagaatt gataaaaagc atcaggtaga ggaggagtcg tatgcgcagg attatagctc
     1021 tcacagctat actggcattt ttaggaggat taatactggg atatgttggt aatagagaga
     1081 acagtataaa ctctgctctg gctcaaacgg agtttttatc aaatgaagaa gcaatttatg
     1141 tgagtattgc agaaaagctt ggtcccagcg tggtgaatat aagtacagtt agtatagtcc
     1201 agtatttctt ccagcctatc cctgcagagg gagctggctc aggggtgata ataagtagag
     1261 atggttatat cattaccaat aaccatgttg tagagggggc aaagagtata aaagttacac
     1321 tggaagatgg acaaactctg gaagggaagc tcatcggaag ggacccattt acagatatag
     1381 cggtaatcaa ggttaatatg accgatttgc catatgcaaa attttcggat ccttccaggc
     1441 ttaaagttgg acagatagct atcgccatag ggaatccatt tggacttgga aagaccgtaa
     1501 cagcagggat agtcagtgcc ctggagagaa gtattcagcc cgatcaggga gtaataatag
     1561 aaagtcttat acagacagac gctgcaataa atcctggtaa tagcggtggc gcgctggtcg
     1621 gaagtaatgg tgagataata ggtattaaca ccgcgatata tcagggggca cagggtattg
     1681 gatttgccat accgagtaat gtagctaaaa agatagctga agacattata gagaaggggt
     1741 atgcaagtca tccatggtta gggataaaag gagaaacact gaatcgaagg acggctcttt
     1801 actatggtct tccgatagat tatggtgtca ttataggaga ggttgtcaaa tccagtcctg
     1861 cagacagagc aggacttaag gttggagatg taattgtagg agtagacaac gagaggatag
     1921 attcatggaa taccttgctt ttaaagattt taaaaactaa aaataatacg gtaacgctac
     1981 agataatgag gaagtccagt aaattagaat taaaagtaca gatagatgaa agaccaaagc
     2041 aatttgctac caatggagga tatttatgat aaaaagcttg aggattttac tgataatatt
     2101 tcttttaatt ccagggctgg cattggctgg agaggtttgg aatctcgaga ccgcacttaa
     2161 gaatatagat aatgcacctg aactaaaagt tcttaaggct caacttgaaa gcgctgattc
     2221 acagctaaaa caggcacagg ctctattctt gccgaagctc aatgggacag ttggatatac
     2281 gaggggtgag agtgcagtaa ataatggaac gattcaggtt gaccagccaa gtgcaacatt
     2341 aaccctgaca tataatctca gtgaaaattc tccggcaggg a
//
LOCUS       DAOTKU010000250         2375 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_36217_length_2375_cov_2.938362, whole genome shotgun sequence.
ACCESSION   DAOTKU010000250 DAOTKU010000000
VERSION     DAOTKU010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36217_length_2375_cov_2.938362"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(90..545)
                     /locus_tag="P5310_06195"
     CDS             complement(90..545)
                     /locus_tag="P5310_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HRU32985.1"
                     /translation="MRCNEVKRSLSAYFDGELSEDKMLKIKEHLKYCNSCASELRKIE
                     RIHGLMESFPVLEAGPYFEAQVIEKIERESTRKRHHLGLKLAIGFALGGILLFLVTFK
                     YQPGQNEPAQSYASLDTYLQEYIETSGRHMAGSNPGLITTISSIGETNR"
     gene            complement(535..1122)
                     /locus_tag="P5310_06200"
     CDS             complement(535..1122)
                     /locus_tag="P5310_06200"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRU32986.1"
                     /translation="MKREEPEEILIKMAKKGDVSAFSELVKRYERYILNLVYRTLGYS
                     EDAEDIAQETFIKAYNNIKKFREESKFSTWLSKIAINLCRDKFRSKSHKEENLEDGVW
                     LTIPQNSYYDPEETAERMEIQEKIRKAVASLPEELRVVFVLRELEDLSYEEISKMLNI
                     PIGTVESRIYRARMKLKTLLSDLRTVEEVTEESEV"
     gene            1298..2350
                     /locus_tag="P5310_06205"
     CDS             1298..2350
                     /locus_tag="P5310_06205"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HRU32987.1"
                     /translation="MEIEKMFYGLTRDEVPVYQYMLINNNGLISKVIPYGCAITELWV
                     PDKNGLLEDIVLGFDTISEYENNTPYLGCIAGRYGNRIARGRFTIDGVTYQLALNEKD
                     KSNHLHGGVKGFRDVVFKAIPMKTPYGPALRMKYLSHDGEEGYPGNLDVTVTYTLTND
                     NEFRIDYFATTDKPTILNLTNHTYFNLAGNCSRDILEHELMLNADYYTPVDEYLIPTG
                     EIAPVKDLAIDFTDPRKIGERIGEYSNPPFGGYDHNFIINRKKSGINLAGILKEYTTG
                     RVMEIYTTEPAIVLYTGNWLNIKGKGGRFYNRYSGLCLETQHCPDSPNHPNFPSAILR
                     LGESFSSTTVYKFGII"
BASE COUNT          708 a          467 c          429 g          771 t
ORIGIN      
        1 tatatctgct ggggtcttat cagctgctga tagttgggat gttatagcta cagtgaagat
       61 taatacaaga aataagtcta ataatttctt cacctattag tttctcctat agaacttata
      121 gtcgtaatca gtcccgggtt actccccgcc atatgtctac ccgatgtctc tatgtattct
      181 tggagataag tatcaagact ggcataagat tgtgcaggct cattttgacc aggctgatac
      241 ttgaaagtta ccaggaagag aagtattccc cctaatgcaa aacctattgc cagtttaagt
      301 ccaaggtggt gtcttttcct cgtgctttct ctctctattt tttctataac ttgagcctca
      361 aaataaggac cagcctctaa gacggggaaa gattccatca gcccgtggat tctttctatc
      421 ttcctcagct ctgaagcaca agagttacag tatttaagat gctcttttat cttaagcatc
      481 ttatcttcag ataattcccc atcaaaatat gctgaaagtg atctttttac ttcattacac
      541 ctcactttct tctgttacct cctccacagt cctgaggtca cttaagagag tctttaactt
      601 cattctggct ctgtatattc tggattccac agtcccgata ggtatattta acatcttgct
      661 gatctcctca taggacaggt cttcaagttc tcttaataca aaaactactc tgagttcttc
      721 cggcaggctt gctaccgcct tccttatctt ctcctggatc tccatccttt ctgctgtttc
      781 ttctggatca taataactgt tttgaggtat tgtgagccag accccatctt ccagattctc
      841 ttctttatga gacttactcc taaatttgtc cctgcataag tttatagcta tctttgataa
      901 ccaggtagaa aacttacttt cctctctaaa tttctttata ttattataag cctttataaa
      961 ggtttcttgt gctatatctt cagcgtcttc tgaataaccc agagttctgt ataccagatt
     1021 taaaatatac ctttcatatc tttttaccag ttcactgaac gcagatacat cgcccttttt
     1081 agccatttta attagtatct cttcaggttc ttctctcttc atctttaacc tctattttag
     1141 atttctaaat taagagacgg aaatcagccc caaaatattc cggaatttta aaatctatac
     1201 aacagtattt attatacaac aataatgtga taatatagag ataaactgaa gagatgctgt
     1261 aatttgcata aatatttggt tataggagga aatgaaaatg gagattgaga agatgtttta
     1321 tggtttgaca agggatgagg ttcctgtcta tcaatatatg ctcataaata ataatgggct
     1381 catttcaaag gttatacctt acggttgtgc cattaccgaa ttatgggtac cggataagaa
     1441 tggattactt gaggatatag tgcttggctt tgatactata agtgaatatg aaaacaatac
     1501 cccatatctt ggttgtatcg ccggacgtta tggtaatcga atagctaggg gtagatttac
     1561 cattgatgga gtaacttatc agctcgccct gaatgagaag gataaatcaa atcatctcca
     1621 tggaggggtt aaaggtttta gagatgttgt cttcaaggct atccccatga aaactccata
     1681 tggacctgct ctcaggatga aatatttaag tcatgatggg gaagaggggt atcccgggaa
     1741 cttagatgtg actgtaacat atactcttac aaatgacaac gaattcagaa tagattattt
     1801 tgctactact gataaaccga ccatacttaa tcttaccaat catacctact ttaatcttgc
     1861 aggtaactgc tccagagata tcttagagca tgaacttatg cttaatgcag attattatac
     1921 accggttgat gaatatttga taccaacagg agagattgca ccggtaaaag atcttgctat
     1981 cgactttact gatccgagga agatagggga gagaataggc gagtatagca acccaccatt
     2041 tgggggttat gaccataact tcataataaa cagaaaaaaa agtggaatta atctggctgg
     2101 tattcttaaa gagtatacca ctggaagagt gatggagata tacactacag aaccagccat
     2161 agtactttat actggaaact ggctaaatat taaaggtaag ggtggaagat tctacaacag
     2221 atattcagga ctctgcctgg agacgcagca ttgtcctgat tctccaaatc atccaaactt
     2281 tccatctgcc atcttaagac taggagaatc attctcttcc acaaccgtat acaaatttgg
     2341 cataatctag gaaaagtatg atgaaggcgg tttat
//
LOCUS       DAOTKU010000251         2374 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_36227_length_2374_cov_2.650280, whole genome shotgun sequence.
ACCESSION   DAOTKU010000251 DAOTKU010000000
VERSION     DAOTKU010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36227_length_2374_cov_2.650280"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1172
                     /locus_tag="P5310_06210"
     CDS             <1..1172
                     /locus_tag="P5310_06210"
                     /inference="COORDINATES: protein motif:HMM:NF016472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ADP-dependent glucokinase/phosphofructokinase"
                     /protein_id="HRU32988.1"
                     /translation="TSAIRKPEDLIIDLLISIREGLAYQRMIENEETFKWTLENVGYD
                     QLRLGGTSANMANLLSPLPLKKILVYANPLTRELAELFPERDNLFILTREDGRPVLRS
                     PRKAWKEEGIFAIHWILEYGEGLKVDLNDLSFQSPRANRFIAAWNPVNCRLRVSEEFK
                     SDIFLIPDRFSHFLVSGFHILLERYPEGKTCEDFIIPVASFVGEMKEKLGVKVHYEFA
                     SIGSQMIRNSVVKNIFPVIDSLGLNEIEFATLLNDLGEQELAKELREGRFPIVKLVDG
                     LIKIMKRTTLRRIQLHTLGHYISITRDKNETEERSALLFSAILAATRSLLGKYETFDD
                     LKKGMEVPTITLTGYEGIKNIDEFGVVIVPTKVVQSPKLTVGLGDIISSSGFLLS"
     gene            1187..2161
                     /locus_tag="P5310_06215"
     CDS             1187..2161
                     /locus_tag="P5310_06215"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HRU32989.1"
                     /translation="MKVYITRRIPEPGIEMIRKEHDVEVNPYDRVLTREELLQAVKGR
                     DGILCLLTDKIDAEFFDAAGPQLKVVSNYAVGYDNIDVDEATKRGIVVTNTPGVLTET
                     TADLAWALIMSVARRIVEADKFTRAGKYEGWAPMLFLGQDVHGKTLGIVGMGRIGQAV
                     ARRAKGFNMKVLYNDIRRIPEELEKELNATFASMDEVIENSDFISLHTYLSPETYHLI
                     NEEKLKRMKKTAYLINTSRGPVIDEAALVKALKEGWIAGAGLDVYEFEPKLVPGLAEC
                     ENAVLLPHIASASVETRTKMATMAAENLLAVLAGRMPPNPVNPEVMKK"
     gene            2158..>2374
                     /locus_tag="P5310_06220"
     CDS             2158..>2374
                     /locus_tag="P5310_06220"
                     /inference="COORDINATES: protein motif:HMM:NF014634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HRU32990.1"
                     /translation="MKILVAPNAFKEALSAREAGEAIKGGLERALPDSDITVLPIADG
                     GDGTIEVLVEATSGKFIERYVTGPLGER"
BASE COUNT          755 a          427 c          616 g          576 t
ORIGIN      
        1 caacttcagc tataagaaaa ccagaggatt taattataga ccttctcatc tctataaggg
       61 aaggacttgc atatcaaagg atgatagaga atgaggagac attcaaatgg acactggaga
      121 atgttggcta tgaccagtta agacttggtg gaacatccgc aaatatggca aaccttctat
      181 cacccttacc cttaaagaag atcctggtct atgccaatcc gctcacccga gagctggcgg
      241 agttattccc tgagagggat aatctcttta tcctgacaag agaagatggc agacctgtcc
      301 tgcgctcccc taggaaggca tggaaagagg aaggaatctt tgccattcac tggatactgg
      361 agtatggtga agggttgaag gtggacctga atgacctctc tttccagtct cccagagcca
      421 acagatttat agctgcctgg aatccagtaa attgcagact gagggtcagc gaagagttta
      481 agagcgatat attcttgata ccagacaggt tttctcattt tcttgtatct ggttttcata
      541 tacttttgga gagatatccg gaaggaaaga cctgcgagga ttttataatt ccggttgcat
      601 ctttcgtggg agagatgaaa gaaaaactgg gggtaaaggt ccactatgaa tttgcatcca
      661 ttggaagcca aatgataaga aattcagtgg taaaaaatat atttccggtt atagatagcc
      721 tcggactgaa tgagattgag tttgcaacac tgctgaatga cctgggtgaa caggaactgg
      781 caaaagaact cagagagggc agattcccaa tagtaaaact agttgatggt ctgattaaga
      841 taatgaaaag aacaactctg aggaggattc agcttcatac cctgggacat tatatctcca
      901 ttacaaggga taaaaatgag acggaagaga ggtctgcact tctcttctcc gctatacttg
      961 cagcgacaag gtctctgctg ggtaaatatg agacgtttga tgacctgaaa aaaggaatgg
     1021 aggtcccgac aatcaccctc accggctatg aagggattaa aaatatagat gagttcggag
     1081 ttgtaatagt tccaaccaag gttgtacaat caccaaaact tacagtaggt ctgggtgata
     1141 taatatcatc atcaggattt ttactaagct aggaggtgta gatagattga aagtatatat
     1201 aacaagacgt ataccggaac ctggaataga gatgataaga aaagaacacg atgtggaagt
     1261 aaatccctat gacagagtgc tgaccaggga agaactactt caagcagtaa aggggaggga
     1321 cgggatactc tgcctcctta cagataagat agacgcagag ttttttgatg ctgcaggacc
     1381 acagttaaag gttgtgagta actatgcggt gggatatgac aacattgatg tggatgaggc
     1441 tacaaagaga ggcatagtgg taaccaatac cccgggagtt ttaacagaga caaccgctga
     1501 tcttgcctgg gctcttatta tgtctgtggc aaggagaatt gtagaggcag ataaattcac
     1561 tagagccggt aaatatgaag ggtgggctcc tatgctcttc ttagggcagg atgttcatgg
     1621 taagactctt ggtatcgttg gtatggggag gatcggacag gcggtagcca ggagagcaaa
     1681 aggtttcaat atgaaggtcc tgtacaatga tataaggaga ataccagaag aacttgagaa
     1741 agagctaaat gccacgtttg cctcaatgga tgaggttata gagaatagcg attttatatc
     1801 actacacacg tatctatcac cagagacata ccacctgata aatgaagaaa agttaaagag
     1861 gatgaagaag accgcctatc ttataaatac atcaagaggt ccagtgatag atgaggctgc
     1921 gctggtaaaa gcactgaaag agggatggat agctggggct ggtctggatg tatatgagtt
     1981 tgagccaaag cttgtccctg gtcttgcaga atgtgaaaat gcagtccttc taccacatat
     2041 agccagtgca agtgtggaga ccagaaccaa aatggctact atggcagctg agaatctcct
     2101 tgctgtattg gctggcagga tgccaccaaa ccctgtaaat ccggaagtta tgaaaaaatg
     2161 aagatactgg ttgctccaaa cgcatttaaa gaggctctct cagcccggga ggctggagag
     2221 gcgataaagg gagggctcga aagagctctc cctgatagtg atattacggt ccttccgata
     2281 gcagatggtg gggatggaac aatagaggtc ctggtagaag caaccagcgg aaaatttata
     2341 gagaggtatg ttaccggacc tctgggagaa agag
//
LOCUS       DAOTKU010000252         2374 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_36230_length_2374_cov_2.518327, whole genome shotgun sequence.
ACCESSION   DAOTKU010000252 DAOTKU010000000
VERSION     DAOTKU010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36230_length_2374_cov_2.518327"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="P5310_06225"
     CDS             complement(<1..410)
                     /locus_tag="P5310_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020389575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32991.1"
                     /translation="MSDDQGLFHISGVVPALSVKAGHLPARTESGIGALMPWADRLWF
                     VSYVSSGPGSGSNTGLFSIDDDFNLKKHPESVVGTYANRMIHSPSCQLIIGPYVIDTE
                     GKVRIIDSIKGHRLTATMEHLEDPEHKVYFLTMEG"
     gene            complement(508..1437)
                     /locus_tag="P5310_06230"
     CDS             complement(508..1437)
                     /locus_tag="P5310_06230"
                     /inference="COORDINATES: protein motif:HMM:NF021619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRU32992.1"
                     /translation="MPENTDKLSKLEVFIKTISKLSGMEICIYDMVYFSYQFENLMLN
                     WQIKSHNSPLCIFTKLDDERHKNCIKIEHNRLYNAINQKEGFLIERCHSGLTDVIIPI
                     SLSGNRLIGGIFFGQVFLDDVPSFSLYEDLFGKDGVIDLVDVKKVINDIPVKSYKELL
                     LWADIGKMIASFVSREIEIWELKSKKEGYLYLSRLDHLLGLSEPIQKAINMIKERSIK
                     GVNLEEISSAVGYSPSYFSKRFKKEVGLGFSEAVRLVKIELAQYLLKTTDKSICQIAF
                     EVGYADFSSFVRAFKKVTGMTPKEYLSHHVPII"
     gene            1570..>2374
                     /locus_tag="P5310_06235"
     CDS             1570..>2374
                     /locus_tag="P5310_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32993.1"
                     /translation="MVRIGSKNTIKKIYHNRDGNIDLWLGSFWNFLIYPESSLDETYD
                     PEVNVSWVYEHLLKRRYPDFVVSVSLDNLLHLPLEDRAKLICISKGIIHDLLPKDQCK
                     LLGFAHNNSLYFCGNLPMSTDEEKKELFQRLNLLIDTLRNRYSLSATIGVAFLPDYSV
                     DSWRWAAQRAVVAQREKVKRGEEKLYVYSDSPSSSLHLIPQDIIRILWDTVNSGNISE
                     VERVSEIVIQELFEKKYFPLIYLRPILQFLTAVMGWAGITAGADITRVFE"
BASE COUNT          742 a          456 c          406 g          770 t
ORIGIN      
        1 ccttccatcg ttaagaaata caccttatgt tctggatctt ctagatgttc catagttgct
       61 gtaagtctat gtcctttaat agagtctata atcctaacct taccctcagt atcaataaca
      121 tatggaccaa tgataagttg acaggatggg ctatgaatca tacgatttgc atatgtccct
      181 actacactct ctgggtgttt ttttagatta aaatcatcat cgatactaaa cagtcctgta
      241 ttagatccag aacctggtcc tgaagacaca taagatacaa accacaatct atctgcccag
      301 ggcatcaacg ctccaatccc actttcagtc cttgcaggta gatgtccagc ctttaccgat
      361 aaggcaggaa ctactccact gatatggaaa agaccttggt cgtcagacat atacactccc
      421 tctctttata tctagatttt tattctaaat taaatatata agaaaagaga ggagtactca
      481 attgcatttt ttgtgataaa tttagcatta aatgatagga acgtgatgag ataaatattc
      541 tttaggggtc atcccagtaa ccttcttaaa tgctcgcaca aaggatgaga aatctgcata
      601 accaacctca aaagctatct gacatatact tttatccgta gttttaagta gatattgtgc
      661 taattctatc tttacaagtc ttactgcttc tgagaaacca agtccaacct cttttttgaa
      721 tcttttacta aagtaagatg ggctataacc tacagcagaa gatatctctt caaggttaac
      781 ccccttaata ctcctttcct ttatcatgtt tatcgccttt tgaataggtt cagaaagacc
      841 taacagatgg tctaatctag agagatagag atatccttcc tttttagact ttaattccca
      901 tatctctatc tctctactta caaatgaagc tatcatcttc cctatatccg cccataagag
      961 gagttccttg taagatttaa cggggatatc atttataacc tttttaacat caactagatc
     1021 tataactcca tctttaccaa agaggtcctc atataaagaa aaagaaggta catcatctag
     1081 aaagacctgt ccgaaaaaga tcccacctat caatctattg ccacttaggc taatagggat
     1141 aattacatcc gttaatccac tatgacatct ctctattaaa aagccttcct tttgattaat
     1201 agcattataa aggcgattat gttctatctt aatacagttt ttatgccttt catcatccag
     1261 cttggtgaat atacataggg gagaattatg agatttaatt tgccagttga gcattagatt
     1321 ctcaaattga tatgaaaagt agaccatatc gtatatacag atttccatac cacttagttt
     1381 cgatatggtc tttataaaga cctccagctt actgagttta tctgtatttt ctggcatctt
     1441 ctctcctttt tacccttcta aaattataca atactttata ttgagtttca ttcctagttc
     1501 atattcgtaa tgtgctagat taaatattgc tgaagtgtta tacttcgagc ataagaggga
     1561 ggacatacgg tggtaagaat aggatcgaaa aatactataa aaaagattta ccataatagg
     1621 gatggtaata tagatctgtg gcttggttcc ttctggaatt ttctgatata ccctgaaagc
     1681 tcactggatg aaacatacga tcctgaagtt aatgtctcct gggtttatga gcatcttctt
     1741 aaaaggagat atccagattt tgttgtaagc gtttctcttg ataatcttct tcatcttccc
     1801 ttagaagata gggcaaaact tatatgcata tctaagggaa tcatccatga tcttctccct
     1861 aaagaccagt gtaagctttt ggggtttgcc cataataaca gtctgtattt ctgtggtaat
     1921 ctgcccatgt caaccgacga agagaaaaaa gaattatttc aaagattaaa tcttttgata
     1981 gatacgctca ggaatcgata ctctctttct gcaaccatag gagtagcttt cctaccggac
     2041 tattctgtcg atagttggag atgggcagct cagagagcag tagtagcaca gagagagaaa
     2101 gttaagagag gagaagagaa attatatgtc tattcagatt ctccatcttc aagcctacat
     2161 cttatacctc aggatataat aagaatactc tgggatacgg taaatagtgg aaatatatca
     2221 gaggtagaga gggtttctga gatagttatt caggagttat ttgagaagaa atatttccct
     2281 ttaatatatc tacgacctat acttcaattt ctcaccgctg ttatgggctg ggctgggata
     2341 acagctggag cagatattac cagggtcttt gaga
//
LOCUS       DAOTKU010000253         2368 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_36347_length_2368_cov_2.961522, whole genome shotgun sequence.
ACCESSION   DAOTKU010000253 DAOTKU010000000
VERSION     DAOTKU010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36347_length_2368_cov_2.961522"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..974)
                     /locus_tag="P5310_06240"
     CDS             complement(<1..974)
                     /locus_tag="P5310_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32994.1"
                     /translation="MKFAVGYQLPEEERESIVDIIEEFRGYIGEVYFPWLDMPSGRSP
                     MTRRDGFVDWEGQYKLEEDLIRLRSMGIELDLLLNANCYGPHSLSINFANFIYSIIEH
                     LKELVSLKIVTTTSLMIAKIVKEKFPDIEVRASVNMRIGTVKAMEYISDLFDSYYIQR
                     EYNRDFQRIEEMKEWAEDNGKKLYFLVNSGCLNFCSGQTFHDNIVAHEDEIVKMNNIK
                     DWDPSICWNYYTKRENWVKFLQNSWIRPEDIHNYDRYFSLAKLATRTHSNPRKVIEAY
                     CKGRFKGNLIDLLEPGHNPIFFPYVIDNSEFPEDWFEKTTRCDKRCHK"
     gene            complement(1002..1973)
                     /locus_tag="P5310_06245"
     CDS             complement(1002..1973)
                     /locus_tag="P5310_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32995.1"
                     /translation="MSTNLTSDLKGYAKKCGADLVGIASMDRFEGAPKQMDPRYIFPD
                     AKALIVLGFRINRGVFRGIEEGTFFAGYPTMGYGGINTVFAPIVLRQVCNFLEDEGYE
                     AVPYHNALVGQDSHFYHTGKPSENFWSHPVSPDKPAPDVFIHFRIAAFCAGLGEIGYS
                     KIFLTPEFGPRQRFVVCLTDAPLEADPLYEGPPLCDRCMLCVRECSGKAISATETIKI
                     NVAGRDLEWGKLDEYKCSVAYTGGVKETNPFLPEDAPDKFEEYSKYLYGGPHYNEFVK
                     WKEPYGYNPAIEGARGCIRACMIHLEQTGKIKNTFKNPFRKKAPWKL"
     gene            complement(1994..>2368)
                     /locus_tag="P5310_06250"
     CDS             complement(1994..>2368)
                     /locus_tag="P5310_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU32996.1"
                     /translation="RGLLPLIQPHFVFPYFWGFYTGAPLYALWYQTGGKEGEQPPPEI
                     LKIMQLYDQFKVETNPAKANSIGKQIIKLSAEQIWYIGTVGLAPTIVIAKTNLRNIPE
                     QSFREAMIGQFSHTRPEQYFFK"
BASE COUNT          671 a          533 c          397 g          767 t
ORIGIN      
        1 catttatggc atcttttatc acacctggta gtcttttcaa accagtcctc tggaaattca
       61 gagttgtcta taacgtaagg aaagaatatc ggattgtgac ctggctccaa taaatctata
      121 aggttacctt taaatcttcc tttacaataa gcttcaatga ctttccttgg atttgagtga
      181 gttcttgtag caagctttgc taatgagaag tatctatcgt agttatgtat atcttcaggt
      241 cgaatccagg agttctgcaa gaactttacc cagttttctc tctttgtgta atagttccag
      301 cagatacttg ggtcccaatc ctttatattg ttcatcttta ctatctcatc ttcatgagcc
      361 acaatattat catgaaatgt ctgtccagag cagaagttca gacaaccact gttcaccaaa
      421 aaatataact ttttaccgtt atcctctgcc cattctttca tctcttctat cctttggaag
      481 tccctgttgt attctctctg aatataataa ctatcaaaga ggtcagagat gtattccata
      541 gcctttactg ttccaattct catattaact gaagccctaa cttcgatatc tggaaatttc
      601 tcttttacta tctttgcaat cataagtgaa gtggtggtca ctatcttaag actaaccagc
      661 tcttttaaat gctcgattat tgagtatata aaatttgcaa aattaataga aagactgtga
      721 gggccgtagc aattggcatt tagaagtaaa tctagctcaa tacccatact tctcagtctt
      781 atcaggtctt cttccaattt atactgaccc tcccagtcta caaatccatc acgtcttgtc
      841 ataggagaac gtccactggg catgtctagc caggggaagt atacctcccc tatatatcct
      901 ctaaattcct caataatgtc aacaatactt tctctctctt cttctggaag ctgatagcct
      961 acggcaaatt tcatagcatt tttattccta ctatccctat gttaaagttt ccaaggagct
     1021 tttttcctga atggattttt aaatgtattc tttatcttac ctgtctgctc cagatgaatc
     1081 atacaggctc gtatgcaacc tcttgcccct tcaatagcag gattgtaacc gtatggctcc
     1141 ttccacttaa caaactcatt ataatgaggt cctccatata aatacttgct gtattcttca
     1201 aacttatcag gagcatcttc aggaaggaac ggatttgtct ccttgactcc tccagtataa
     1261 gcaaccgaac atttatattc gtccaatttc ccccattcaa ggtctctccc agctacgtta
     1321 atttttattg tttctgtggc acttatagct tttccagaac attctcttac gcatagcata
     1381 catctatcac agagtggagg accttcatat agaggatcag cctcgagagg agcatctgtt
     1441 aggcagacaa caaatctttg cctaggtcca aactcaggag tgagaaatat tttactatac
     1501 cctatctccc caagcccagc acaaaaagca gcgatacgga agtggataaa aacgtcagga
     1561 gctggtttat caggagacac aggatgactc caaaaatttt cacttggttt tccagtatga
     1621 tagaagtggg agtcctgacc tacaagggca ttatgatagg ggacggcttc ataaccctca
     1681 tcttctaaaa aattacatac ctgtcttaaa acaatggggg caaatactgt gtttatacca
     1741 ccatatccca tagttggata cccggcaaaa aaagtaccct cttcgatccc cctaaataca
     1801 cctctattga tacgaaatcc taagactatc aatgcctttg catctgggaa tatgtatctg
     1861 gggtccatct gcttgggagc tccttcaaat ctatccattg atgcaatccc aactaaatct
     1921 gccccacact tcttagcata tccctttaga tcacttgtaa ggtttgtaga cattattctg
     1981 cctcctacat taattattta aaaaagtatt gttcaggacg tgtatgagag aactgtccta
     2041 tcatagcctc tctaaaagat tgttcaggaa tatttcttag attagtttta gcaattacaa
     2101 tagtaggcgc taatcccaca gtacctatat accaaatctg ttccgctgaa agcttaataa
     2161 tttgcttccc gatggaatta gccttggctg gatttgtctc tactttaaac tgatcgtata
     2221 actgcataat ctttagtatc tctggtggtg gttgctctcc ttccttccct cctgtttggt
     2281 accagagggc atatagaggt gcgccggtat aaaatcccca gaaatatggg aatacaaagt
     2341 gaggttgaat cagtggtaga agacctct
//
LOCUS       DAOTKU010000254         2367 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_36368_length_2367_cov_3.239619, whole genome shotgun sequence.
ACCESSION   DAOTKU010000254 DAOTKU010000000
VERSION     DAOTKU010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36368_length_2367_cov_3.239619"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="P5310_06255"
     CDS             <1..230
                     /locus_tag="P5310_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011228579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HRU32997.1"
                     /translation="EKKITDQAFGRINVYDLVMKRLAQMKSNPEKFDPFTGPIKDQSG
                     KVRIETGRMATHDELWTMDWFVPNVIAKIPK"
     gene            complement(280..1362)
                     /locus_tag="P5310_06260"
     CDS             complement(280..1362)
                     /locus_tag="P5310_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009662456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HRU32998.1"
                     /translation="MSVRELTEKLEDISLSPYATKSRVAVRKEEESPCPVRTAFQRDR
                     DRIIHSKAFRRLKHKTQVFFPTESDHFSTRMTHTLEVAQIGRTIARALRLNEDLVEAI
                     ALGHDLGHTPFGHIGEDVLDSIYREKLGIPFHHAEQSVRVIEILESRLTPEERRGLNL
                     TVQVIDGIENHSKGSQDLESLLGYPSKVTTFEAHTVRISDRLAYLNHDVDDAVRAGII
                     SEDDIPEDIVRVLGRNRSERVNTMVQSLVEASLGKPFFDMDREVTKALDSLKDFLYER
                     VYKNPDVRRWEGGARTVIQRLFEFYELHPEHINLGSKDREEVIWEIGDYLAGMTDIFA
                     LRSYYEIFGNGTPFPNILYLLEKIKG"
     gene            complement(1368..>2367)
                     /locus_tag="P5310_06265"
     CDS             complement(1368..>2367)
                     /locus_tag="P5310_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012965791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HRU32999.1"
                     /translation="LKGAMGAVENGYGWAEDLERIEENGAMEGADPAVISAKARERGA
                     PQLGTLGSGNHFIEIQRVQKIFDPEIAKIFGLFEDQVTILIHTGSRGFGHQVCTDYIS
                     VMMSAIKKYNISLPDRQLACAPIESSEGKNYFSAMVCAANYAWANRQCITHWIRESFE
                     VVFRRGAHKLGLVVLYDVAHNIAKMEIHKLDGEDVRVCVHRKGATRAFPKKHPEIPEV
                     YREVGQPVIIPGDMGRASYILVGTEKAMETTFGSTCHGAGRVMSRAGAKRSTTGREVL
                     TELAEKGIMVKTDNIKGLAEEASEAYKDVERVIEIVEGSGISKRVARMKPMGVIKG"
BASE COUNT          590 a          598 c          408 g          771 t
ORIGIN      
        1 aagagaagaa gattacagat caggcatttg gcaggataaa tgtatatgac cttgtaatga
       61 agaggctggc tcagatgaaa tccaatcctg aaaaatttga tccatttaca ggaccgataa
      121 aagaccaatc aggaaaagtc aggatagaaa caggtagaat ggctactcac gatgagctct
      181 ggactatgga ctggtttgtt ccaaatgtta tagccaagat acctaaatag actcttatca
      241 gataattctc aagtgagggg taggaggact ttgtatcttc tatcccttta tcttttctaa
      301 gaggtaaagg atattcggga atggagttcc atttccgaat atctcgtagt aagacctgag
      361 tgcaaagata tctgtcatac cggcaaggta gtctcctatc tcccatatga cttcttccct
      421 atctttagaa cccagattta tatgttctgg atgtaactcg tagaattcaa ataatctttg
      481 aataactgtt ctggctcctc cttcccatct tctcacgtct ggatttttat ataccctctc
      541 atacaaaaag tctttcagtg aatccagggc ttttgttacc tctctgtcca tgtcgaagaa
      601 gggtttacct agagaggctt ctaccagact ttgcaccatg gtgtttaccc tttctgatcg
      661 attcctcccc aggactctca ctatgtcttc tggtatatca tcttctgata ttattccagc
      721 tcttaccgca tcatctacgt catggtttaa gtatgccagc ctatctgata ttctgactgt
      781 atgggcttca aatgttgtaa cctttgaagg gtatcccagt aaactttcca ggtcctgact
      841 acctttagag tgattctcta taccgtctat aacctgaaca gtaaggttca gccctcttct
      901 ttcctcagga gtaagtctac tttccaaaat ctctataacc cttacactct gctctgcatg
      961 atggaaagga atcccaagct tttctctgta tatactatcc aggacatctt ctcctatatg
     1021 tccgaaaggg gtatgtccca gatcatgacc cagtgctatg gcttccacca ggtcttcatt
     1081 tagcctcaaa gctctggcta tagtcctacc tatctgtgcg acctctaatg tatgtgtcat
     1141 tctggtacta aaatggtcac tttctgtggg aaagaatacc tgcgtcttat gctttaacct
     1201 tctaaatgcc ttggaatgaa ttatccgatc cctgtctctt tgaaaggcgg tccttacagg
     1261 acagggagac tcttcttctt ttcgaacagc aactctactc tttgtagcat atggtgataa
     1321 agatatatct tccagtttct ctgtaagttc tcttactgac atttcgacta accttttatt
     1381 actcccatag gtttcatcct tgctaccctt ttagatatac ccgagccttc tactatttct
     1441 ataaccctct ccacatcttt ataagcttca gaagcctctt ctgccagtcc ttttatgtta
     1501 tctgtcttta ccattatccc cttctcagca agttctgtca ggacttctct gcctgttgtc
     1561 gaccttttgg caccagccct actcatcact cttcctgccc catggcaggt tgatccaaat
     1621 gtggtttcca tagctttttc tgtcccaacg agtatataag aggctcttcc catatctccc
     1681 ggtataataa caggttgtcc cacttccctg taaacctcag gtatctctgg atgctttttg
     1741 ggaaacgccc tggtagcacc ctttctatga acacataccc ttacatcttc cccgtccaat
     1801 ttatgtatct ccatctttgc tatattgtgg gcaacgtcat ataaaactac cagtcccagc
     1861 ttatgggcac ctcttctgaa gacaacctca aaactctccc tgatccagtg tgttatgcac
     1921 tgcctattag cccaggcata attggcggca cataccatgg cagaaaagta attctttccc
     1981 tccgagcttt ctatgggagc acaggctaat tgtctatccg gtaaagagat attatatttt
     2041 tttatagcac tcatcataac agaaatataa tcagtacata cctgatgccc gaagcctctg
     2101 gaccctgtat gtatcagtat ggttacctgg tcttcaaata atccaaatat ctttgctatc
     2161 tcagggtcga agattttttg aaccctctgt atttctataa aatgatttcc agaccccaga
     2221 gttcccagct ggggcgcacc tctctctctg gctttagcac ttatgacagc tggatctgcc
     2281 ccttccatag ctccattctc ttctatcctt tccagatctt ctgcccatcc atagccgttt
     2341 tctactgccc ccatggcacc ttttaac
//
LOCUS       DAOTKU010000255         2352 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_36663_length_2352_cov_4.359164, whole genome shotgun sequence.
ACCESSION   DAOTKU010000255 DAOTKU010000000
VERSION     DAOTKU010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36663_length_2352_cov_4.359164"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="P5310_06270"
     CDS             complement(<1..459)
                     /locus_tag="P5310_06270"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HRU33000.1"
                     /translation="MKWLIFLISPSGTWGRVRDSNWVFIPLIVGLVSALSNFYITNNP
                     QVIYLKAETILKLSRDSFFTLPEGEGVIRLVTSLDFLLLPLWYTVIVILAGYMTKLMV
                     KNIDYKKVMLISSLSILPVIPVRIILTWFLQSKGLNYLTDLRDLNLTLGPT"
     gene            complement(461..>2352)
                     /locus_tag="P5310_06275"
     CDS             complement(461..>2352)
                     /locus_tag="P5310_06275"
                     /inference="COORDINATES: protein motif:HMM:NF039504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5696 domain-containing protein"
                     /protein_id="HRU33001.1"
                     /translation="DDGYTMMSSPPDWKSVNDYWDSRIRSMFIVEYWDRGLENSRMTF
                     SYDAKRSVKYTGKTIEIEYRFPDIGLSFTSVIENRDDCFLVKIPDSTFRENENYPVIG
                     ITFLPYLNAGRVGERGYIVIPDGCGTMIQFNKFFGVTTLEKSVYGDLTPSNYNINNIP
                     ITMPLFSCIKNNLGFAGIISEGKEFSGLVMDMAPGNGYYSIGTKFYYRKIFEDVYRKK
                     RITQRRDNKDRSILFSLLKDESASYMGVGRTYREYLLKEKKIKTLKEKVKKYNYIDPE
                     IIDINIFMGIKRGFQLFDPVIKLTTFKDIGWMLNRLKSLGIKANLILTGWERSGFEGE
                     NPVTFPPFDGLRGFKAIVDKAKASGSFISLSVNYFEIYQNSRDFYRRMTLKSPVKLPV
                     EQSNYRNGFIICPEVAMSKFQKFYSEAKKNGVTDVELRRLGRGLIECFDDEHPADRFE
                     SARIYSEMMENAKIVDGGCDYGVGGVNTFMFAPSTSSGFFGGEDIPLWQIVYHGMVRY
                     SFEPVNLRKDFDYEFLKSLEYGALPSAMLTYESPILIRETFYKDLFSSKFSDWERELK
                     NEYELFNKKLGDVQYEFIVDHREVARYVYQTTYSDGTRVIVNYSNEDYNGIKPLGYKI
                     VRGRY"
BASE COUNT          699 a          516 c          370 g          767 t
ORIGIN      
        1 agttggacca agggttaaat tcaaatccct gaggtcggtt aagtaattca gccctttgga
       61 ttgaaggaac caggtcaaga ttatcctaac cgggatcact ggtaagattg atagggaaga
      121 gataagcata acttttttgt agtcaatatt tttaaccatc agttttgtca tatatcctgc
      181 aagaataact attactgtat accatagagg cagtaagaga aagtccagag aagttaccaa
      241 ccttattacc ccctctcctt ctggaagagt aaaaaagcta tctctggata attttaagat
      301 ggtttcagcc ttcaggtata taacctgtgg attattggta atgtaaaagt tacttaatgc
      361 cgataccagt ccgacaatta aagggataaa cacccagtta ctatctctaa ctctacccca
      421 ggtaccagaa ggagagatta aaaatataag ccatttcatc ctagtatctt cctcttacta
      481 tcttataacc taagggtttt ataccattgt agtcttcatt ggaataattg acaataaccc
      541 tggtaccatc actatacgtt gtttggtaga catacctggc tacctctctg tgatcaacta
      601 taaactcata ctgcacatcg cccagtttct tattgaagag ctcatactca ttttttaatt
      661 ccctctccca gtctgaaaat tttgagctga acaggtcttt atagaaggtc tctcttataa
      721 gtatggggga ttcatatgtc agcatagcac tgggtagagc accgtactcc agtgatttga
      781 gaaactcata atcaaaatct ttcctgaggt ttacaggctc aaaagaatac ctcaccatac
      841 cgtgatatac aatttgccag agggggatat cttctcctcc aaaaaagcct gaagaggtgg
      901 aaggagcaaa cataaacgta tttacacctc ctacaccata atcacatcct ccatccacta
      961 tctttgcatt ttccatcatc tccgaataga tcctggcact ttcgaatcta tctgcaggat
     1021 gttcatcatc aaagcattca attaaacctc tacctaaccg tcttaactcc acatctgtta
     1081 ccccattctt cttcgcttca ctgtaaaatt tttggaattt agacatcgct acctcggggc
     1141 atataataaa accatttcta tagttgctct gttctacagg tagctttaca ggactcttca
     1201 gtgtcattct cctgtagaaa tctcttgagt tctgatagat ttcaaaataa ttgacagaga
     1261 gacttataaa agatccggaa gccttagctt tatccactat agccttaaat cctctgagac
     1321 catcaaatgg agggaaagta actggatttt ctccctcaaa gccagacctt tcccatccgg
     1381 taaggatcag gtttgctttt ataccaagac tctttaatct attcagcatc cagcctatat
     1441 ctttaaatgt ggtaagcttt attacagggt caaacagctg aaatcctctc tttataccca
     1501 taaatatatt tatgtctatt atttctggat ctatgtaatt atactttttt accttttctt
     1561 ttagtgtttt aattttcttc tcttttaaca gatattctct gtatgtcctg ccaacaccca
     1621 tatagctggc ggattcatcc ttcaataaag aaaagagaat acttctatct ttattatctc
     1681 tcctttgagt tatacgtttt tttctgtata cgtcctcaaa tatcttcctg taatagaatt
     1741 ttgttccaat agaataatac ccgtttccgg gagccatatc catcaccaga ccgctaaact
     1801 cctttccttc agatattata ccagcaaagc ccagattatt ctttatacag ctaaataaag
     1861 gcattgtaat tggaatattg ttgatattat aatttgaagg ggtcaggtct ccatatacag
     1921 atttttccag tgtagtcaca ccaaagaatt tgttaaactg aatcatagtc ccacaaccat
     1981 cggggattac aatataacct ctctcaccga ccctgccagc attgagatat ggtaaaaagg
     2041 ttatgccaat taccgggtag ttttcgtttt ctctgaatgt actgtcgggt atctttacca
     2101 gaaaacaatc atctctgttt tctattactg atgtaaaact gagacctata tcaggaaatc
     2161 tgtactctat ctctattgtc ttcccagtat atttaactga ccttttggca tcataagaga
     2221 acgtcatcct ggagttttcg agtcctctat cccagtattc aactataaac atcgacctta
     2281 tcctggaatc ccaataatcg tttacagact tccagtctgg aggagaactc atcatggtat
     2341 atccatcatc tc
//
LOCUS       DAOTKU010000256         2342 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_36886_length_2342_cov_3.686926, whole genome shotgun sequence.
ACCESSION   DAOTKU010000256 DAOTKU010000000
VERSION     DAOTKU010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36886_length_2342_cov_3.686926"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="P5310_06280"
     CDS             <1..327
                     /locus_tag="P5310_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU33002.1"
                     /translation="WENTWCPLWAQWYATKGKSGEEPPKEVKTLLTLWEKMQTTLSDK
                     ERIRLGKEILKSNAENLWTIGIAGLAPQPIIAKNYLRNIPEKGLWGYDNLRTYPYFPS
                     QIFIKK"
     gene            complement(468..1616)
                     /locus_tag="P5310_06285"
     CDS             complement(468..1616)
                     /locus_tag="P5310_06285"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRU33003.1"
                     /translation="MLSELEYVLELLQPDPERIELGRKRQGSVWRGEEPDYIPVTPPP
                     IDVPEKEKFNSYNYKEQFYDPEKMLIMHLWELIGRARIPGDSQLSMRANLGTGFVPTV
                     FGLNQLIFEDKMPWLKEHLNKDIILSIKPEEITDDFVITRGLVPRAIEYINFFKEKLE
                     RKAHVYLSDTQGPLDIAHLVYGDMFFTDIYDDPEFIHHLLKITTNAYISVSRVLKRAI
                     GEELNSGYHTVLYMENGGVRCCEDTTTLLSPKTFKEFVAPYISSALKPFGGGWVHFCG
                     SGHQFLEDLLSMPEVKGINFGNPERYDYYSTMSLILDHKKFYFGNFPRKEEESLEDYF
                     KRILRPFSERGTRSGMIFMPFIEDLPEWRETPEKVLELWYSLQDKLIG"
     gene            1806..>2342
                     /locus_tag="P5310_06290"
     CDS             1806..>2342
                     /locus_tag="P5310_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRU33004.1"
                     /translation="MDIDLFVRFKSKIIENVSRVIVGKDEVIELVTVAFICGGHILLE
                     DVPGLGKTLLVKAFAKTIGCDFKRIQFTPDLLPSDLTGINFYNQKTGDFQFRPGPIFT
                     NILLADEINRATPRTQSALLEAMQEQQVTVDGVTRKLPEPFMVLATQNPIESYGTFPL
                     PEAQLDRFFMRIKIGYPTR"
BASE COUNT          674 a          474 c          476 g          718 t
ORIGIN      
        1 tgggaaaata cctggtgccc tctatgggct cagtggtatg caaccaaagg taagtctgga
       61 gaagaaccac ctaaagaggt aaagactcta ttaactctct gggaaaaaat gcaaacgact
      121 cttagcgata aggaaaggat aagactgggg aaagaaattc tcaaatctaa tgcggagaat
      181 ctatggacta tcggtattgc tggtttagcc ccgcagccaa ttatagcaaa gaactatctc
      241 agaaatattc ctgagaaggg actctggggt tatgataatc tcagaactta tccatatttc
      301 ccctctcaga ttttcattaa aaaataagag aactttggag gaggtttata cctcctcctt
      361 taatgatttg agctatatca ggaaagatta tacggaaatt actcagaacc ttttagcctg
      421 taacaaataa caccattaga gcctctttta ttctggtacc ccagccccta tcctatcagt
      481 ttgtcctgaa gagagtacca gagctctaat actttttctg gggtctctct ccattctgga
      541 aggtcttcaa tgaatggcat aaagatcata ccgcttcttg ttcctctttc actgaatggt
      601 cttaagatcc tcttgaagta gtcctctaaa gattcttcct ctttccttgg gaaattacca
      661 aaatagaatt ttttgtggtc aagtataaga ctcatagtgg agtagtagtc atacctctcc
      721 ggatttccga aatttatacc cttcacttca ggcatagata aaagatcttc cagaaactgg
      781 tgtccgcttc cacagaagtg gacccatccc ccaccaaatg gcttaagtgc agatgatatg
      841 tacggggcta caaactcttt gaacgttttt ggagataaca gggtagttgt atcttcgcaa
      901 catctcaccc caccattttc catatataag acagtgtgat atccagagtt cagctcttct
      961 cctatggctc ttttgagtac tcttgacaca cttatatatg cattggtggt aattttcagg
     1021 agatgatgta tgaattcagg gtcatcgtat atgtccgtaa agaacatgtc tccatagact
     1081 agatgggcta tatcaagggg accctgagtg tctgaaaggt agacatgggc ttttctctca
     1141 agtttctcct taaagaagtt tatatactct attgctcttg gaacaagccc tcttgttata
     1201 acaaaatcat ctgtaatctc ttctggcttt atagatagta taatatcttt attgagatgt
     1261 tcttttaacc agggcatctt atcctcaaag ataagctgat tgagtccaaa aactgttgga
     1321 acaaaacctg ttccaaggtt agctcgcatg gaaagctgac tgtcaccagg tattcttgct
     1381 cttcctatga gctcccatag atgcataatg agcatcttct cagggtcata gaattgctct
     1441 ttatagttat aggagttaaa tttttctttc tccggaacat ctatgggtgg tggtgttaca
     1501 ggtatatagt ctggctcttc acctctccat acactgccct gtcgttttct accaagttct
     1561 atacgctccg gatcaggctg aagcagttcc aaaacatatt caagttctga caacatacaa
     1621 atcctccttg ttaattacta caaataggac ataaaacagt aattttactg tatattttac
     1681 cattttctca cccctttgtg ttataattac tacgagtgat gaagttcacc ataactttcc
     1741 ctgaaggaga ggttgatggc ttctattcat tttgagtaga agccattttt ttgcaggagg
     1801 cttgaatgga tatagattta tttgtgaggt ttaagagtaa aataatagag aatgtatcca
     1861 gggttattgt gggtaaggat gaggtaatag agctggttac agtagctttc atctgtgggg
     1921 ggcatatact tttggaagat gttcccgggt tgggtaagac cctgctggta aaagcctttg
     1981 ccaagactat tggttgtgac tttaagagga ttcagtttac ccccgactta ttaccatcag
     2041 atttaacagg aataaatttc tacaatcaaa agaccgggga ttttcagttt agaccgggac
     2101 ctatattcac aaacatactt cttgctgatg agataaacag ggcaactcca aggacacagt
     2161 ctgcacttct ggaggcgatg caggagcagc aggtgacagt tgacggggta accagaaaac
     2221 tgccggagcc atttatggtc ctggccacac agaaccctat agagtcttac gggacattcc
     2281 cccttcctga ggctcaactt gatagatttt ttatgaggat aaagataggt tatccgacca
     2341 ga
//
LOCUS       DAOTKU010000257         2340 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_36957_length_2340_cov_1.733042, whole genome shotgun sequence.
ACCESSION   DAOTKU010000257 DAOTKU010000000
VERSION     DAOTKU010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36957_length_2340_cov_1.733042"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>408
                     /locus_tag="P5310_06295"
     CDS             <1..>408
                     /locus_tag="P5310_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU33005.1"
                     /translation="AVDFYVVDPASDRWHNLILETVKNLVDCGVSGVYLDELAMAGAF
                     QDIPDWIRGYTAMLDKIVERYPDKVFIIEGCSDIYGRYVDLQLVSTFFYWYTSYPELF
                     RYTFPDRILVDMLYPEIQTMRPAFVSGISKELLN"
     assembly_gap    409..418
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <419..803
                     /locus_tag="P5310_06300"
     CDS             <419..803
                     /locus_tag="P5310_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU33006.1"
                     /translation="FILGMYPWIYDLEVDNRFEDTDYLKEYLKLREIGMDFFNGGKFL
                     DDLGIETVDDIRAKLYSKEDCYMVAVYNRDKIRGKLEFKCLTPPLEVEIVSSERIRYR
                     SDSNIIEVPQEERSLIFISKPGGIE"
     gene            800..1942
                     /locus_tag="P5310_06305"
     CDS             800..1942
                     /locus_tag="P5310_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HRU33007.1"
                     /translation="MKTLYGLDVLEEDDFSILKGKRVGLITNYSGVSSHLRVNVELML
                     ASGIKIEKIFTPEHGFFGASDGLEIRDSIHPKYGIRMISLYGDKKAPEREDLEDIDVL
                     VYDIQDVGLRFYTFIYTLAYSLEAASKYGLPYIVLDRPNPLGGEIIRGPRIRDDLNSF
                     VGGYGLPIRYGLTIGELAFYFKKFKMLDLDLKVIKMKNWTRDRYYPDTGLFWNVPSPN
                     IPAFSSTICYTGMCFIEATNISEGRGTTKPFEYIGAPWIDEDAVYDELRREDFPDIAF
                     RKRYFIPEFSKYKGEVCRGLEFFPLNYNADFVRVALSFISIVARKFPEHFSLRRYNEV
                     LSIDLLIGDKETEKEILEGKSWRTIYERWREEEEAFKDFVKDLRLY"
     gene            complement(1928..2335)
                     /locus_tag="P5310_06310"
     CDS             complement(1928..2335)
                     /locus_tag="P5310_06310"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007917504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HRU33008.1"
                     /translation="MIKGDAEGYIVFEDDISIAFLDVRPLNPGHCLLVPRGHYETLMD
                     IPDELIAPLFQNAKLISKAIEVGLEAQGIFVGINNRVSQSVPHLHIHIVPRNRGDNLK
                     GFFWPRYPYKDRETILDIQNRIRGAINRLLIQP"
BASE COUNT          693 a          368 c          534 g          735 t
ORIGIN      
        1 gctgtagatt tttatgtggt tgaccctgct tctgacaggt ggcacaatct tatattagag
       61 actgtaaaaa atttagtgga ttgtggagta tctggtgtat atcttgatga gttggctatg
      121 gcaggagcct ttcaagatat tccggattgg atcagaggat acactgcgat gttagataag
      181 atagtggaaa gatatccgga taaagtattc attatagaag gttgcagtga tatatatgga
      241 agatatgttg atcttcaact ggtctcaacg ttcttttact ggtatacgag ttatcctgag
      301 ttattcagat acaccttccc tgaccgtatc cttgtagata tgctgtaccc ggagattcaa
      361 accatgagac cagcttttgt atctggaata tcaaaagaat tactcaacnn nnnnnnnnat
      421 ttatccttgg gatgtatccc tggatatatg accttgaagt ggataaccgt ttcgaggata
      481 cagattattt aaaagaatat ttgaaactga gagagattgg tatggacttt tttaatgggg
      541 gcaaattttt ggacgatctg gggattgaga ctgtcgatga tataagagct aagttgtaca
      601 gtaaggaaga ctgttatatg gttgctgtat ataacaggga taaaatcagg ggtaaattag
      661 aatttaagtg tttgacccct ccgcttgagg tagaaattgt ctcttccgaa aggatacggt
      721 acaggagtga tagtaatata atagaggttc ctcaagaaga gaggtctctt atatttatct
      781 ctaaaccggg aggcattgaa tgaagacact ttatgggttg gatgttttag aagaagatga
      841 tttttcaatc ctgaaaggaa agagggttgg acttataaca aactactccg gtgtatcatc
      901 tcacctgaga gtaaacgtgg agctcatgtt agcttcaggt ataaagattg aaaagatctt
      961 tacccctgaa catggatttt ttggtgcatc tgatggactt gagataagag attctataca
     1021 tccaaagtat ggtataagga tgataagcct ttatggcgat aagaaggctc ctgaaagaga
     1081 agacctggag gatatagatg tcctggtcta cgatattcag gatgtgggat tgagattcta
     1141 tacttttata tataccctgg cttattctct ggaggctgcc agtaaatatg gattacccta
     1201 catagttctg gatagaccta atccacttgg aggggagata attagaggac caaggataag
     1261 ggatgacctg aactcttttg tcggtgggta tggactccct ataagatatg ggcttacaat
     1321 cggagaatta gctttttatt ttaaaaaatt taagatgtta gacctggact taaaagttat
     1381 taagatgaaa aactggacca gagataggta ttatccagat acaggtcttt tctggaatgt
     1441 tccctctcca aatatccctg ctttctccag taccatatgt tatacaggga tgtgctttat
     1501 agaggctacc aacatatctg aggggagagg aacaacgaag ccttttgagt atataggtgc
     1561 tccctggata gatgaagatg cggtctatga cgagctgagg agagaagatt tccctgatat
     1621 cgctttcaga aagaggtact ttatccctga gttttccaaa tataagggtg aggtgtgcag
     1681 gggtttagaa ttctttcctc taaattataa tgcagacttt gtaagggttg cactttcttt
     1741 tataagtata gtggcaagga agtttcccga gcatttttct ctcaggagat ataatgaagt
     1801 attgagtata gatctcctta taggggataa ggagacagaa aaagagatct tagagggtaa
     1861 aagctggaga acaatctacg agagatggag agaggaagag gaagccttta aagattttgt
     1921 aaaagattta aggttgtatt aacagcctat ttatagcccc tcttatcctg ttttgaatat
     1981 ctaggattgt ttctctatcc ttatacggat atcttggcca gaagaatccc tttagattgt
     2041 ctcctctatt tcttgggact atatgtatat ggaggtgtgg aacgctctgg cttactctgt
     2101 tatttatccc aacaaatatc ccctgcgcct ctaatccaac ctctatggct ttagatatga
     2161 gttttgcatt ttggaataga ggagcaatta actcgtcagg gatatccatc agggtttcat
     2221 aatgtcctct cggtactaag agacaatgtc ctggattcag tggtcttaca tctaaaaatg
     2281 ctatggatat gtcatcttca aaaactatat atccttctgc atcacctttt ataatctttc
//
LOCUS       DAOTKU010000258         2337 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_37007_length_2337_cov_3.185364, whole genome shotgun sequence.
ACCESSION   DAOTKU010000258 DAOTKU010000000
VERSION     DAOTKU010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37007_length_2337_cov_3.185364"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1430)
                     /locus_tag="P5310_06315"
     CDS             complement(<1..1430)
                     /locus_tag="P5310_06315"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRU33009.1"
                     /translation="MLREINIRGFNIIDSVNATFTPGLNIVTGETGAGKSLIVEAIQQ
                     LLGARASSGMVKSGYPKAQIIGIFDLPKREELIRLLEENGIEVADELIVLREITSTGK
                     NSIKVNGRAIPASVMRETGRYLVNIFSQMENAEIFSPEYQLELIDSYGRLHSYRDSFN
                     EFFSKWRDLREKKLSIEERIKTRLQEIDFLNYQIDELRRANLVENEDEMLEEEESRLL
                     NLERLKNDIEKSYLLLYEDDDSVISRLSRVSQILTGLGDIDPQLGDLARDLENQGILI
                     GEVSRNIRTYMNHLNEVDSSKLEELEARIHFINELKRKYRVKSVNELLEELKKLESRR
                     NGLEKIQEESSNLDSIIEETSNQLKSLGEELTYRRRETAEKLSTLLEEELKRLGMPDV
                     RIRLEVEETVEPAPSGFNMVTFLIATVPDEPLKRIEDVVSGGELSRISLAIRSLLRDV
                     ESFPTFIFDEIDIGIGINTGDAIVGN"
     gene            complement(1423..2256)
                     /locus_tag="P5310_06320"
     CDS             complement(1423..2256)
                     /locus_tag="P5310_06320"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HRU33010.1"
                     /translation="MKIAIFPNPHREEALILGSDIVSYIKSKDVYAKLFDSWAEDVTE
                     DFDIGIVIGGDGTLLRASKILVPLGVPVLGIRVGGLGFLFDVEPDEYKDAIDKLLNKD
                     YRIESRGTLDITVEKSGITKGKFLALNDAVVNKGTFARMLEITLYVNKNLVARYPADG
                     IIVSTPTGSTAYSLSAGGPIVSPDLNCVLVTPICPHSLFLRPILLNPRDMIEIVVGEK
                     HEEVMLTVDGQLGVHLDTEDRIKVSFSNSQWKLIRLSKSPDFFSTLREKLHWGERVGD
                     A"
BASE COUNT          598 a          560 c          366 g          813 t
ORIGIN      
        1 atattcccta caatggcatc gcctgtgttt atgcctatac ctatatctat ctcgtcaaat
       61 ataaatgtgg ggaaagactc gacatctctg agaagacttc ttattgcaag agatatcctg
      121 gataactctc caccagaaac gacgtcttca atccttttta gaggttcgtc agggacagta
      181 gctataagaa acgttaccat attgaatcca gaaggtgcag gttcaacagt ctcctcaact
      241 tctaacctta ttcttacatc tggcatccct aacctcttta attcttcttc taaaagagtg
      301 gaaagcttct ctgcagtttc tctccttcta tatgttaatt cctcacccaa agacttcaac
      361 tgattggatg tctcttctat aattgaatct aaattgctac tctcctcttg aattttttcc
      421 agtccatttc ttctactctc taattttttt aactcctcta ataactcatt tactgatttt
      481 accctgtact ttctcttaag ttcatttata aagtgaatcc ttgcttctaa ttcttccagt
      541 ttagaactgt caacctcatt taaatggttc atataagttc tgatgttcct cgacacctct
      601 cctatcagta tcccctgatt ttcaaggtct ctcgcaagat cccccagttg agggtctata
      661 tctcccagtc ctgttaatat ctgtgacacc ctggataatc tggaaattac tgagtcatca
      721 tcttcgtata agagaagata agacttttct atatcattct ttagcctttc cagatttaga
      781 agtcgtgact cttcctcttc tagcatctcg tcttcgtttt ccacaagatt ggctcgtctc
      841 aattcatcta tctgataatt gaggaagtca atctcttgga gcctggtttt tatcctctct
      901 tctatgctca gtttcttttc cctgagatct ctccacttag agaagaactc attgaatgag
      961 tctctatagc tgtgcagtct tccataagaa tctataagct ccagctgata ttcaggagaa
     1021 aagatctctg cattttccat ctgggagaat atgttgacca gatatcttcc tgtctctctc
     1081 attacagaag cggggatagc tctcccattt acctttatgc tgttcttacc tgtactggtt
     1141 atctccctta atactatgag ttcatctgcg acctcaatcc cattctcctc cagtagtctt
     1201 atcaattctt ctctcttagg caggtcaaat atccctatta tctgagcttt gggataaccc
     1261 gactttacca taccagagct ggctctagct cccagaagtt gctgtatagc ttccactatc
     1321 agagatttac ctgcaccggt ctctccggtg actatgttta accccggggt aaaggtggcg
     1381 tttacactat ctattatgtt gaatccccta atatttatct ccctaagcat ctcctactct
     1441 ctctccccaa tggagcttct ctctcagggt agagaaaaag tctggacttt tgcttaaccg
     1501 tattaacttc cactggctgt tactaaagct tactttgatt ctatcctctg tatcaaggtg
     1561 tactcccagt tgtccatcta ctgttaacat aacttcttca tgcttttctc ccacgactat
     1621 ctctatcata tctctgggat ttaatagtat aggtcttaaa aatagagagt gtggacatat
     1681 tggggtaacc agtacacagt tcaggtcagg ggatacgatt ggtcctccag ctgatagaga
     1741 ataagcggtt gaacctgtag gggtagatac tataatgcca tcggcaggat atcttgccac
     1801 cagattttta tttacataca gggttatctc caacatcctg gcaaatgtac ctttatttac
     1861 gactgcgtca ttcagagcaa ggaatttccc tttagtaatt ccgctctttt ctactgttat
     1921 gtccagtgtt cctctgcttt cgatcctgta atctttgttc aataatttat ctatagcatc
     1981 cttatattca tctggttcta catcaaagag aaaccctaac cctccaactc ttattccgag
     2041 tacagggacc cccagtggta cgaggatttt tgatgccctc agtaaagttc catctccccc
     2101 tattactatc ccaatatcaa aatcttctgt aacatcttca gcccaggaat caaaaagctt
     2161 tgcataaaca tctttagatt ttatgtagct tacaatatct gaacctaata taagagcctc
     2221 ttctctatga ggatttggga atatcgctat tttcaaaata tttaaaagcc tcctctactg
     2281 tagagttaat atcaggttta cttatgcttt ctctccttga gagatacaat aggtatt
//
LOCUS       DAOTKU010000259         2334 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_37059_length_2334_cov_2.546292, whole genome shotgun sequence.
ACCESSION   DAOTKU010000259 DAOTKU010000000
VERSION     DAOTKU010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37059_length_2334_cov_2.546292"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1339
                     /locus_tag="P5310_06325"
     CDS             <1..1339
                     /locus_tag="P5310_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L-fucose/L-arabinose isomerase family protein"
                     /protein_id="HRU33011.1"
                     /translation="GRERILKVLEEEGFNVITFGPSDGKFGAVETLEHSINCAELFKT
                     NSEKIEGIIVTLPNFGDERSIANTLRFSKLDVPVLVHAFPDDLNKMDVAHRRDSFCGK
                     LSVCNNLNQYGIPFSLTKLHTVAPESKSFREDLKWFNSVCKVVNGLKNARIGLIGARP
                     AAFNTVRFSEKLLENYGISVEPIDLSEIFDGISKLPDNDSAVREKVERLRNYMDTAGV
                     PENAILKMAKFSVVIDKWMKENKLVASAIQCWSAIENIYGIVPCAVMSMMSESLLPSA
                     CETDVPGVIAMYALQLASGTPSVIVDWNNNYGEDPDKAVVFHCSNYPKSFLKEPKMTF
                     QDIISGTVGKENAYGSCAGRISSGPMTFARVSTNDLIGTIQAYVGEGEFTDDPLSTFG
                     GYGIAHVEGLQELLRIICERGFEHHVAMNKSNSAEVLYEAFSNYLGWDVYWHK"
     gene            1422..2315
                     /locus_tag="P5310_06330"
     CDS             1422..2315
                     /locus_tag="P5310_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate 5-dehydrogenase"
                     /protein_id="HRU33012.1"
                     /translation="MKRIVSISLGSSKRDHRVSLSILGEEVIIERIGTDGDIDKAIEK
                     IRELDGKVSAIGLGGIDLYFTIGNKRYVVKDALKMAKAAVKTPVVDGSLIKDTLEKQT
                     VKLLSNVIPGGIRGKNVLLPSAVDRFGMAEAFHNLGANILIGDLMFALGIPIPIYSIK
                     LLKILAYILLPVLTRLPFYILYPVGEKQEKNTPKFTKYFIWADIIAGDFIYIKKYMPE
                     RLEGKVIFTNTVTSGDKEMLKERGVRYLITTTPELGGRSFGTNVLEGFLVAMQDKRLP
                     QESDEEFLERIGFKPRVEIPN"
BASE COUNT          779 a          370 c          547 g          638 t
ORIGIN      
        1 aggaagagaa agaatactaa aagtcctgga agaagaagga tttaacgtta ttacatttgg
       61 tcccagtgat ggaaaatttg gggcagtaga gacattagaa cattctataa attgtgcaga
      121 gttatttaag acaaatagtg aaaagataga gggtataata gtaactctac ccaacttcgg
      181 agatgaaagg tctatagcaa ataccttaag gttctcaaaa ctggatgttc ctgttttagt
      241 acacgcattc ccggatgacc ttaacaagat ggatgttgca catagaaggg atagtttctg
      301 tggtaagcta tctgtatgta acaacctgaa tcagtatggt atcccgttct ctcttacaaa
      361 acttcataca gtcgcaccgg aaagtaaaag cttcagagag gatttaaaat ggtttaacag
      421 tgtctgcaag gtcgtaaatg gcttaaagaa tgccaggata ggacttattg gggcaagacc
      481 tgccgcattt aacactgtca gattcagtga aaaattactg gagaactatg gaatctcggt
      541 ggagcctatc gacctctcgg aaatctttga tggcatctca aaacttccag ataacgatag
      601 tgccgtcaga gaaaaggttg aaaggttaag aaattatatg gatactgcag gtgttccaga
      661 aaacgccata cttaagatgg ctaaattcag tgtggtcata gataagtgga tgaaagaaaa
      721 taagcttgta gcaagtgcta tacagtgctg gagcgccata gaaaatatat atgggattgt
      781 tccctgtgct gttatgagta tgatgagcga atcactctta cccagcgcct gtgagacaga
      841 tgtacctgga gttatagcca tgtatgcact ccagctagca tcaggaacac caagcgttat
      901 agtagattgg aataataatt atggagaaga ccctgataaa gcagtggtat tccactgcag
      961 taattatcca aagtctttct taaaagaacc aaaaatgacc ttccaggata tcatatcggg
     1021 gactgttgga aaagaaaacg cctacggatc ctgcgcaggt agaatctcga gtggtcctat
     1081 gacatttgcc agagtatcta ccaatgactt gattggaact attcaggctt atgtgggaga
     1141 aggagaattt actgatgacc cattatcaac ctttggcggt tatggtatag cacacgtgga
     1201 aggattacag gagttactcc gtattatatg tgagagagga tttgagcatc atgtagcaat
     1261 gaataaatct aactctgctg aggttctgta tgaagctttc tctaattatc ttggctggga
     1321 tgtatattgg cataaatagt aaataaaatc agtataaggg ggaaagggtt taattccccc
     1381 ttatacttgt aagattaaaa tatttctgat aaaatctcta tatgaaaaga atagtaagca
     1441 taagcctggg ttccagtaaa agagaccaca gggtctcttt gtctattttg ggagaagagg
     1501 taataataga aagaataggg acagatggag atatagataa agctattgag aagatcagag
     1561 agcttgatgg aaaggtctcg gctataggtt tggggggcat agatctatac tttaccatag
     1621 gtaataaaag gtatgtggta aaagatgcgt tgaagatggc aaaagctgca gtaaagactc
     1681 ctgtagttga cggaagttta ataaaagaca cactggagaa gcagacagta aaactattaa
     1741 gtaatgtgat tcctggagga atcagaggga aaaatgtact cttaccctct gccgttgaca
     1801 ggttcggtat ggctgaagca ttccataacc tgggggctaa tatccttata ggagacctta
     1861 tgtttgcatt ggggattcct atccctattt acagtataaa attactgaaa atcctggctt
     1921 atatattact ccctgtgctg accagattac ctttttatat attatatcca gtcggagaaa
     1981 aacaggagaa aaatacacct aaatttacga aatactttat ctgggcagat ataatagctg
     2041 gagattttat ctacatcaaa aagtatatgc ctgaaaggct ggagggtaag gttatattta
     2101 ccaatacggt tacctctgga gataaagaga tgcttaaaga gagaggggta agatatctta
     2161 taactaccac tccagaatta ggcggcaggt cctttggcac caatgtgctg gaagggtttt
     2221 tagtggctat gcaagataag cgacttcctc aggagagcga tgaggagttc ttagaaagaa
     2281 taggatttaa accaagggta gaaataccta attgattcaa agaggtgata gtgt
//
LOCUS       DAOTKU010000260         2333 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_37078_length_2333_cov_2.671642, whole genome shotgun sequence.
ACCESSION   DAOTKU010000260 DAOTKU010000000
VERSION     DAOTKU010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37078_length_2333_cov_2.671642"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            70..1050
                     /locus_tag="P5310_06335"
     CDS             70..1050
                     /locus_tag="P5310_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU33013.1"
                     /translation="MNESLAVDFIKKLRAHQIWWDDSVREPINIKEDIPPADVETNVE
                     ISGEWKAIETEKIDHRKKIVIVDGVRRIDAHLILGDAGKSALLGEFAVGAFSLEDGKV
                     IERIVECVIVLPPGISSSHPLSIGKAEYIPFSGGDSTEEGLYSSFIQRMRERERTLAF
                     EFSRSGYTVIADGPLQVPFSEVGVTLGFIKSIQRYYLYGKYWDTVLSLRRGERTPIFL
                     IRTGEDLPPKYSWYLRLKEPKYFESPFFGLVRLEAPSNLDIEYVKSLAQFAGGFLPYI
                     ISSSTLSSRAPENLAPLESLERRLRAMFHPADHIRATLRQLIAQAYEEVK"
     gene            1047..>2333
                     /locus_tag="P5310_06340"
     CDS             1047..>2333
                     /locus_tag="P5310_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU33014.1"
                     /translation="MNVGIVLGTRESTPFEFYIEVDGNQTLQVDDIIVTTCEYPRFGR
                     VTFYGIVDQAMSRLEGLETNEQARFHSQGLFDANLTYYCHIVVTRTLAEDGSSPKIPP
                     RPGSEVRLADERDTELALYFDKMEKRKVPCGLLSNNTPAYLDLDFILGIKGGHINISG
                     KSGVATKTSYATFLLYSYFSNLQEIKRTYPEVGLTNTHAVIFNVKGEDLLFLDTPNSK
                     LSDKDRKMYEAMGLKAKPFESVKFYAPPRPKRGQYDAILPNTESRLKGVNVFAWTMRE
                     IGEEDLLWYLFSPKDLSDTSQLYLAIRIATNWLNRELKDGLKFQSIKSMIQTLEESIE
                     DVMGRVASGTRDAFMRRLYRAEQEIGPLIVPETLPFDVETESYSIDWRSAQVNVIDIH
                     KLSPRAQYFTVGAVLKSIVEEKEKTGTPWPLVFIVLD"
BASE COUNT          757 a          397 c          553 g          626 t
ORIGIN      
        1 aagatatagc aaaatatttt ctctttcatg agcttatatc cagaccaatc tccctgagaa
       61 aataaggcta tgaacgagtc cctggcagtt gactttataa agaaattaag ggctcatcag
      121 atatggtggg atgattctgt aagggaacca ataaatataa aagaggacat ccctcctgct
      181 gatgtggaaa ccaatgttga aatttctgga gagtggaagg cgatagagac agaaaaaata
      241 gaccatagga agaaaatagt gatagttgat ggagtaagga ggatagatgc ccacctgatc
      301 ctgggagatg caggaaaatc tgcacttctt ggagagtttg ccgtgggagc tttcagcctg
      361 gaggatggta aggtgataga aagaatagtt gaatgtgtaa tcgtcttgcc accgggaata
      421 tctagctcgc atcctttatc tatcggaaag gctgagtata tcccattttc cggaggtgat
      481 tctaccgaag agggacttta tagctctttt atacagagga tgagagagag agaaaggacg
      541 ctggcatttg aattttcaag gtctggttac actgtaattg cagacggtcc tctacaggtt
      601 cctttctcag aagttggagt gacacttgga tttataaaaa gcatacagag atattattta
      661 tacggtaaat actgggatac agtactatca ttaaggagag gagaaagaac tccgatattc
      721 cttatacgga caggagaaga tttgcccccc aaatactcct ggtacttaag attgaaggaa
      781 cctaaatatt ttgaatctcc atttttcggg ctggtaaggc tagaagcccc atcgaatctg
      841 gatatagagt atgtaaaaag tctggctcaa tttgcaggag gatttttgcc gtatataata
      901 agcagcagta ctttaagcag tagagcccca gaaaatttag cccctctaga aagcttagag
      961 aggagattaa gggcgatgtt tcatcctgca gatcatataa gggctactct caggcaatta
     1021 atagcccagg catacgagga ggttaaatga atgtcggtat agtattggga actagagaat
     1081 ctactccttt tgaattctat atagaggtgg atggtaatca gacactgcag gtagatgata
     1141 taatagttac aacctgcgag tatcccagat ttggcagggt aactttctat gggatagtag
     1201 accaggcaat gtccagacta gaaggtctgg agactaatga acaggcaaga tttcactctc
     1261 aaggactttt tgatgccaat cttacatact actgtcacat tgtggtcact agaacattgg
     1321 ctgaggatgg ttcaagtcct aaaattccac cgagaccggg ctcagaagtg agacttgccg
     1381 atgaaagaga tactgaactt gcactatact ttgataagat ggagaagagg aaggtcccct
     1441 gtggactctt atccaacaat acacccgcat atctggacct tgattttatt ctaggaataa
     1501 agggtggaca tataaatata agtgggaaaa gtggagtagc cacaaagaca tcttatgcta
     1561 catttcttct ttactcctat ttttctaatc ttcaagagat aaagaggaca tatcctgagg
     1621 ttggactgac caatacacat gcagttatat ttaatgtaaa gggggaagac ctgctctttc
     1681 ttgatacacc aaactcaaaa ttgagtgaca aagacagaaa aatgtacgag gctatgggtt
     1741 taaaggctaa acctttcgaa agtgtaaaat tttatgcccc accaagacct aagagaggac
     1801 agtatgatgc tattctccca aatacagaga gcagactgaa aggggttaat gtttttgcct
     1861 ggacgatgag agagattgga gaagaagacc tgttatggta tctgttttct ccaaaagatc
     1921 tttcagatac aagtcagtta tatctggcta taagaatagc tacaaactgg ctcaatagag
     1981 aattaaaaga tggacttaaa tttcaatcta taaaaagtat gatccagaca ctagaggaaa
     2041 gtatagaaga tgtgatgggt agagtggcat ctggaaccag agatgcattt atgagaaggt
     2101 tgtacagggc ggaacaggag ataggtcctc ttatagtccc ggagaccctc ccatttgatg
     2161 tggaaacaga atcttatagt atagactgga gaagtgctca ggtaaatgta atagatattc
     2221 ataaattatc tcccagggct caatatttca ctgtaggtgc tgtactgaag tcgatagttg
     2281 aggagaagga gaaaaccgga actccctggc ctttggtctt tatagtgctg gat
//
LOCUS       DAOTKU010000261         2329 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_37159_length_2329_cov_2.802111, whole genome shotgun sequence.
ACCESSION   DAOTKU010000261 DAOTKU010000000
VERSION     DAOTKU010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37159_length_2329_cov_2.802111"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /locus_tag="P5310_06345"
     CDS             <1..587
                     /locus_tag="P5310_06345"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRU33015.1"
                     /translation="GWAISREGLLKILEDAKRNDILVVIDEAYSEFMKESLSCLIKEY
                     DNIIILRTFSKAFSLAGLRLGYAVSNREIINDLMAVKLPYNVDIFSQMVGRIVLKYKD
                     MILPRVEEIIKERNRLYESLSRLSGVRVFPSDANFLFFYCEDESLMDRLLSYKIKIRK
                     FEPRHPLLKNAYRITVGRREDNDRFIFAMEEILT"
     gene            584..1480
                     /locus_tag="P5310_06350"
     CDS             584..1480
                     /locus_tag="P5310_06350"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HRU33016.1"
                     /translation="MRPEIIVFDIDGVLIDTLPSFRVAVSRTVQYYITEILKYDDTGL
                     FILPEEVQFFKIAGGFNDDWVLSQSIILFFIYKAITSGLKDTRSVRLIPPSFEDFTRD
                     ISCIGEGLEKVEKYISNMDNNIISEIKSRLDKELVIRIFQEIYAGSEDMKRLYGSEPL
                     SGKEEGLWRRERIILDKALLIPDLFYGIYTGRTASETELALNMLKLDIPPQAIITMDS
                     SIIKPDPRGLEIIRSYYKREILAFVGDSMDDILTAINGNAIPVAIAKDDISRERFSHY
                     TSLIFNNVNEYLMSLNKIHLSL"
     gene            1546..1848
                     /locus_tag="P5310_06355"
     CDS             1546..1848
                     /locus_tag="P5310_06355"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRU33017.1"
                     /translation="MDKSKEPVYLISVTARLLGVHPRTLRIYEEWGLVTPKRIGNRRF
                     YSEEDLEKLRLITCLTSKVGMSLAGVKLILRLEEELSSQDMGDVFIDILKEILENF"
     gene            1866..>2329
                     /locus_tag="P5310_06360"
     CDS             1866..>2329
                     /locus_tag="P5310_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HRU33018.1"
                     /translation="MALRRWDPFRELESLRREMDRIFDEVFGRASFSGRESELEPIEE
                     YARAWTPACDIYETDKEVVVKANLPGVSKDNVEVVVTEDAVTIKGEAKEESEVKEKNY
                     YRKELSYGAFQRVIPLPVPVKSDEAKATFKDGILEIRAPKQEVSPKGKKINI"
BASE COUNT          757 a          351 c          553 g          668 t
ORIGIN      
        1 caggctgggc tatatctaga gaagggttat tgaagatact ggaagatgct aaaagaaacg
       61 atattctggt tgtaatagat gaggcttaca gtgaatttat gaaggagagt ctttcctgtc
      121 tgataaaaga atacgacaat atcatcatac tcaggacatt ttccaaggct ttttctctgg
      181 ctggtttaag acttggatat gcagtttcta acagggagat tataaatgat ttaatggctg
      241 ttaagcttcc ctataatgta gatatcttct cgcagatggt aggtagaatt gttctgaagt
      301 ataaggatat gatccttccc agggtagaag agattattaa agagaggaac aggctctatg
      361 aatctctctc aaggttatct ggagtcaggg tctttccatc tgatgctaac tttctcttct
      421 tttactgtga ggatgaatct ctgatggaca ggttgttatc ctataaaatc aagataagaa
      481 aatttgagcc aagacatcca ttactaaaaa atgcatacag gattacagtt gggagaagag
      541 aggataatga taggtttata tttgctatgg aggagatact gacttgagac cggagattat
      601 agtttttgat atagacgggg tattaataga taccttaccc tctttcaggg ttgcagtttc
      661 caggactgtt caatactata taacagagat tttaaaatat gatgatacag ggctatttat
      721 actcccagaa gaggttcaat tttttaagat agctggagga tttaatgatg attgggtgtt
      781 atctcaatct attatccttt tcttcattta taaagctata acatcaggat taaaagatac
      841 cagatcagtt agattaatac cgcccagttt tgaggatttt accagagata tctcttgtat
      901 tggagaagga ttggaaaagg tggagaaata tatatcaaac atggataaca acatcatttc
      961 agaaataaag agtagacttg acaaggaact ggtcatcagg atctttcagg agatatacgc
     1021 tggttctgaa gatatgaaga gattatatgg ttcagagcct ctttcaggaa aagaggaggg
     1081 gctatggagg agagagcgga taatactgga taaggcactt ctcataccag atctgtttta
     1141 tgggatatat acaggtagga ccgccagtga gaccgagctg gctctgaaca tgttgaaact
     1201 tgatatacct cctcaggcta tcataactat ggattcttca attataaaac ctgatccaag
     1261 agggcttgag ataataaggt catactataa aagagaaatt ctcgccttcg taggagactc
     1321 tatggatgac atattgaccg ctataaatgg taatgctata ccggtggcga tagctaaaga
     1381 cgatatctcc agagagagat tctctcatta taccagtctc atcttcaaca atgtgaacga
     1441 gtatctgatg tctttgaata agatacacct ctcactttag agaaagttgt aaatggtatt
     1501 gacaactttt ttataatatg ctattatatc taccgtgaaa gtgatatgga taagagtaaa
     1561 gagccagttt acttaataag tgtaactgca aggcttcttg gggttcatcc caggaccttg
     1621 cgaatatatg aagagtgggg acttgtaacc ccaaagcgta taggtaacag gcggttttac
     1681 tcagaagaag atttggagaa gctgagactc attacctgcc tgacaagcaa ggtgggtatg
     1741 agtctggctg gggtaaagtt gatattacgc cttgaagagg agttatcctc tcaggatatg
     1801 ggtgacgtat ttatagatat ccttaaagag atattagaaa acttttaaaa ggaggtgtta
     1861 gtagtatggc tttaagaaga tgggatccat ttagagaact tgagtctctt agaagagaga
     1921 tggatagaat atttgatgag gtctttggaa gggcttcatt ctctggaaga gagtctgaat
     1981 tagaaccgat agaggaatat gcgagagcct ggactccagc atgtgacata tatgagaccg
     2041 ataaagaagt ggtggtaaag gctaacctgc ctggagtcag caaggacaat gtagaggtag
     2101 tcgtaacaga ggatgctgta acaatcaaag gagaggctaa ggaggaaagc gaggtaaagg
     2161 agaagaacta ttatagaaaa gagctcagct atggcgcttt ccagagagtt atacctctac
     2221 ctgttcctgt aaagtcagac gaggctaagg ctacatttaa ggatggtatt cttgagataa
     2281 gagcccccaa acaggaggtt tctcctaaag ggaaaaagat taatatcga
//
LOCUS       DAOTKU010000262         2328 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_37178_length_2328_cov_2.969644, whole genome shotgun sequence.
ACCESSION   DAOTKU010000262 DAOTKU010000000
VERSION     DAOTKU010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37178_length_2328_cov_2.969644"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            209..1345
                     /locus_tag="P5310_06365"
     CDS             209..1345
                     /locus_tag="P5310_06365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HRU33019.1"
                     /translation="MRILHTGDWHIGINRWGIDRSEEVFRSIDFIVKTVEEEDIDVIL
                     IAGDLFDHRTPKGENLQRATRILQKLGESERKVIVVTGNHDWNDLEASLNMFSSLSNV
                     YFLTQIGVMSFETKKGETLRLGVLPYFWERDFMFMGGETARRENLGERLRGGLDKINR
                     GFLPDTVNILLAHTFIEGAVLGSAMRLSFSSNFALPPSWLPDRAHYVALGHAHKPQKI
                     QNSPVPAYYSGSIIRIDFSELDDKKSFNIIDVKPDRPAVIEQREIPCRELMELEIKLS
                     QLESKAILVKDFDGYIKVKLVVDVTDKNPISRVKQLIPNCIEVETVYPAQSTGSVSSV
                     DLTSEPSKLFHQYLILKDGRVDDEVIKLFEELYREVSSDENPEH"
     gene            1326..>2328
                     /locus_tag="P5310_06370"
     CDS             1326..>2328
                     /locus_tag="P5310_06370"
                     /inference="COORDINATES: protein motif:HMM:NF024573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HRU33020.1"
                     /translation="MKILNIELAGFLSYSTPQFVDFTGANVFCISGPNGAGKSSILDG
                     IVYALYGKIPRYAGRRVNTEDDIINHNSDRLSLSLKFKVGERVFLVRRELVKGKSQQA
                     NIFELIDEKAQPLGAKRNRDVNAFIENLLGMDYETFTRTVILPQNQIDRFLKPASSEA
                     LSERRQVLQRLLGLDIYKEIKKLANQKCRDISRELQMISDRLEGELKNYTREFVRDLE
                     KKLKEKEIILKTSEEERQSLSSKIEELKSAIALFESHISTLEMYKDITARLNMLKGKK
                     EEADRLEVLYQLQREAIPLSNLNREYNEKKNRLDSLKREELKQEDERERLYRELEKEK
                     "
BASE COUNT          757 a          397 c          572 g          602 t
ORIGIN      
        1 cccagagggt ctctgtgggt gggcatatca ctctctctat ccgtttatcc atgccctcag
       61 acatacgaaa tcttatgaag attcaggaca caggctggtt aagaaggata cagtagatgt
      121 tgcctgtcct gacaatgcat gggttgtatt tgagatagaa aaactgtaat ctggtagggg
      181 tggatcaagc cacccctatc taaaagagat gagaatactt catacaggtg actggcacat
      241 aggtataaat agatggggaa tcgacaggag cgaggaggta ttcaggtcta ttgattttat
      301 agtaaaaacc gtcgaggaag aagatataga cgtaattctt atagctggag acctgttcga
      361 tcacaggacc cccaagggag agaatcttca aagagccacc aggatactgc agaaactggg
      421 agaatctgag aggaaggtta tagttgtaac agggaatcac gactggaatg acctggaagc
      481 cagcctgaat atgttctcat ccttatccaa tgtttatttc ctgacccaga taggggtgat
      541 gagttttgag acgaaaaaag gggaaactct gcgcctgggg gttcttccgt atttctggga
      601 gagagacttt atgtttatgg ggggagaaac tgccaggaga gagaatttgg gggaaagact
      661 cagaggagga ttggataaaa tcaatagagg attcttgccg gacaccgtaa atattcttct
      721 tgcccatacc tttattgagg gtgctgtcct tggcagtgcg atgagacttt cattctcctc
      781 caattttgct ctaccgccat cctggctccc tgacagggct cactatgtcg cactgggaca
      841 tgcccataag cctcaaaaga tacagaacag tcctgttcca gcttattatt ccgggtccat
      901 aataaggata gatttcagtg agctggatga taagaagagt ttcaatataa tagatgtaaa
      961 gccggataga cccgccgtta ttgaacagag agaaattccc tgcagggaac ttatggaatt
     1021 agagatcaag ctctcgcagc tggagagcaa ggctattctg gttaaggatt tcgatggata
     1081 tataaaggta aaactggttg tggatgttac ggataaaaac cccataagca gggtaaaaca
     1141 actcatcccg aactgtattg aagtagagac tgtatatccg gctcaatcca ctgggagtgt
     1201 ctccagcgta gaccttacca gtgaaccttc aaagctcttc catcaatatc ttatccttaa
     1261 agatggaagg gtcgatgatg aagtgattaa acttttcgaa gagttataca gagaggtatc
     1321 ctctgatgaa aatcctgaac attgagctgg ctggtttttt atcttacagt accccccagt
     1381 tcgtggattt tactggagcc aatgtcttct gtatctcagg tccaaatggg gcagggaagt
     1441 catcaatact tgatggcata gtttatgccc tgtatggcaa gatacccaga tatgctggta
     1501 gaagggtgaa cactgaggat gatattataa accataattc tgatcggctt tctctttctc
     1561 ttaaatttaa ggttggagag agggtatttc ttgtaaggag agagctggta aaaggaaagt
     1621 cacagcaggc taatatattt gagcttatag atgagaaggc tcaacctctt ggtgcgaaga
     1681 ggaatagaga tgtaaatgcc tttatagaga atcttctggg gatggattat gaaacattta
     1741 ccagaacggt tatcctgcca cagaatcaga tagacaggtt ccttaaacct gccagcagtg
     1801 aggctctatc ggagaggagg caggttttac agcgtcttct tgggctggat atatataaag
     1861 agataaaaaa attagccaat cagaaatgca gagatatctc cagagaactt cagatgatat
     1921 ctgataggct tgagggtgaa ctgaaaaatt ataccagaga atttgtaagg gatttagaaa
     1981 aaaagttaaa agaaaaagag attattttga agacatccga agaggaaagg cagagcttaa
     2041 gcagtaagat agaagagctg aaaagtgcaa ttgctctttt tgaaagccat atctctactt
     2101 tagagatgta taaagatata actgccagat taaatatgct taaaggtaaa aaagaagagg
     2161 cggatagact tgaggtatta tatcaactgc agagagaagc catacctctt tctaatttaa
     2221 atagagaata caatgaaaag aagaacaggc ttgattcttt aaagagagaa gagttaaaac
     2281 aggaagatga aagagaaaga ctttacaggg aactggaaaa agaaaaga
//
LOCUS       DAOTKU010000263         2320 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_37347_length_2320_cov_4.514349, whole genome shotgun sequence.
ACCESSION   DAOTKU010000263 DAOTKU010000000
VERSION     DAOTKU010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37347_length_2320_cov_4.514349"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="P5310_06375"
     CDS             <1..185
                     /locus_tag="P5310_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRU33021.1"
                     /translation="DGKMWTITVGIVSFSSQYGGLEMWGPMFAGYVIASIPLIILFFF
                     TMRYFIAGLTSGALKA"
     gene            192..1283
                     /locus_tag="P5310_06380"
     CDS             192..1283
                     /locus_tag="P5310_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRU33022.1"
                     /translation="MTSRERFINILEYKPVDRGIYGIGVGTWPETIERWKKEGYDPSK
                     EPLFDRDHWEWIGHWFFPDPPFERKVISEDDRTITYINHEGILMRERKDNPMSSMPQF
                     IRFPVETREDFQKFMKERMQPDLASRIGKDYVKILQGYRERNFPLVIISDRWGGFFGP
                     LRNLVGVERLCMLFYDDSAFVEEMMDAFADFIIAMMDKILDYTDIDMYGFWEDMCYKT
                     GPLISPELVRKFMYPRYRRVVDFLHSRGVKWISLDSDGKITSLIPIWLDGGINFIYPF
                     EVQSGMDVINVRKTFGKGLRMMGGIDKRAIADGPGAIDSELKRVAPLVEEGGYVPAPD
                     HSLPPDVSFGNYCYFMERLKDLVTGDMRL"
     gene            1280..>2320
                     /locus_tag="P5310_06385"
     CDS             1280..>2320
                     /locus_tag="P5310_06385"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRU33023.1"
                     /translation="MKEMTKKERVIATLNLQETDRVPIYDLLYNDAVIEYFTGKIPPP
                     GEEGLKITCEAISRMLDMTRSVTGPSEPGIFIRDDGFVIRRDRWTSWIEERPFKDEDG
                     AKRWLVESIERLRNADVKEIADRYLEYFTKILNYIGDTIALLAQTGTGLDELRHDLGI
                     ELFSYLSVDEPELISEYLDAYTDLQVSFIDKIADKNLSPCVLTYGDIAIKGRLLHSPE
                     WLRREFLPRLKRLNDAWHRHDIKCLFHSDGYIMDIIPDLIETGIDGLNPIEVVAGMDL
                     REVKRLYGDSLFIAGGIDVSQLLSNGTAEEVRKICKEAIDIGYPGYFIGSTTELDNGA
                     KLENVLAMVETAW"
BASE COUNT          731 a          334 c          564 g          691 t
ORIGIN      
        1 ctgatggcaa aatgtggact ataactgtag gcatagtaag cttctctagc caatatggag
       61 gtttagaaat gtggggacct atgtttgctg gatatgtgat agcttctata cctcttatta
      121 tacttttctt ctttacaatg agatatttta tagcgggatt aacttctggt gcactgaaag
      181 cgtagcctga gatgacatct cgtgaacgtt ttataaatat cttagagtac aagcctgtag
      241 atcgaggtat atatggaata ggggtgggta cctggcctga aacaatagaa cgttggaaga
      301 aagaaggata tgatccatcc aaagaacctc tcttcgatag agaccactgg gaatggatag
      361 gtcactggtt ctttccagac ccaccttttg aaagaaaggt catttcagaa gatgatagaa
      421 ctataacata tataaatcat gagggcattc ttatgagaga aaggaaagac aatccgatgt
      481 cctcaatgcc acaattcata cgttttcctg tagagacaag ggaagatttc caaaagttta
      541 tgaaagaaag gatgcagcca gatcttgcat ctaggatagg gaaagattat gttaaaatat
      601 tacagggata tagagagagg aatttcccac tggttatcat ctctgataga tggggaggtt
      661 tttttggtcc attaaggaat ctggttggtg ttgaaagatt atgtatgctt ttctacgatg
      721 actcagcatt tgtagaggag atgatggatg cctttgctga ttttatcatc gctatgatgg
      781 ataagatatt agattatacg gatatagata tgtacggttt ttgggaggat atgtgttata
      841 agaccggtcc attaatcagt ccagagctgg taaggaagtt tatgtatccg agatatagaa
      901 gggtggtaga ttttttacat tccagaggag tgaaatggat ctctttagat agcgatggta
      961 aaattacatc tttgattccc atatggcttg atggaggaat aaattttatt tatccattcg
     1021 aggtccaatc tgggatggat gttataaatg ttcgcaagac ctttgggaaa gggctaagga
     1081 tgatgggagg tatagataaa agagctatag ctgatggtcc aggtgcaatt gattcagagc
     1141 ttaaaagagt agctccttta gtagaagaag gaggatatgt gcctgctcct gatcacagtt
     1201 tacctccaga tgtatcgttt ggtaactact gttactttat ggagagattg aaggatttag
     1261 taactggaga tatgagacta tgaaagagat gacaaagaag gaaagggtaa tagccacatt
     1321 aaatcttcaa gaaacagaca gggttcccat ttatgatcta ctctataacg atgctgttat
     1381 agagtacttt acagggaaga ttcctcctcc cggtgaagaa ggtttgaaga taacctgcga
     1441 ggctatatcc aggatgctgg atatgactag aagcgttact ggtcctagtg agcctggtat
     1501 ctttattaga gatgatggct ttgtgataag acgtgatcgt tggacaagct ggatagaaga
     1561 gagacctttt aaagatgaag atggtgcaaa gagatggtta gttgagagta tagaaagatt
     1621 aagaaatgca gatgtaaaag aaatagctga tagatatctt gagtatttca cgaaaatact
     1681 taactatatt ggagacacaa tagctcttct tgctcagaca ggtacaggac ttgatgagct
     1741 tagacatgac cttggcatag agcttttctc ttatctatct gtagatgaac ctgaactcat
     1801 ctctgagtat ttagatgcat atacggatct acaggtaagc tttatagata agattgcaga
     1861 taaaaatcta tctccctgtg tacttaccta tggtgacatt gccataaagg ggagattact
     1921 tcactctcct gaatggttga gaagagaatt cttgcccaga ttaaagagat tgaatgatgc
     1981 ctggcataga catgatataa aatgtttatt ccattctgat ggatatataa tggatataat
     2041 tcctgacctt atagagactg gaatagacgg attgaatcct attgaagtag tagctggaat
     2101 ggacctaaga gaggtcaaga ggttgtatgg agacagtctc tttattgccg gaggtataga
     2161 tgtaagtcaa ttactatcca atggtactgc tgaagaggta aggaagattt gcaaagaggc
     2221 tattgacatt ggatatcccg gatattttat aggttctact accgaattgg ataatggggc
     2281 aaagttagag aatgttctgg ctatggttga gacagcatgg
//
LOCUS       DAOTKU010000264         2312 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_37511_length_2312_cov_3.100576, whole genome shotgun sequence.
ACCESSION   DAOTKU010000264 DAOTKU010000000
VERSION     DAOTKU010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37511_length_2312_cov_3.100576"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="P5310_06390"
     CDS             complement(<1..737)
                     /locus_tag="P5310_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRU33024.1"
                     /translation="MRLIRVSILLLSVLLIVNIGSGSLAGKITVTDLAGRRVEVPIPA
                     RRIVAIGPGALRLVCYVNGVNRVVGIENIDREGSPGGKTYLIAYPELKKLPVIGQGGA
                     FSSPDPEKIVNVKPDVIFACSFIDRKGAEDLQLKTGIPVVMLDYGISTIINESLYKSL
                     RLTGMIIGEEKRAGDIVNYIKKTQRELDLRTEKIADGKKPGVYVGALGMKGGHGIEST
                     QAKYPPFVMVNARNVVDETGREGSIMI"
     gene            complement(734..1273)
                     /locus_tag="P5310_06395"
     CDS             complement(734..1273)
                     /locus_tag="P5310_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013404166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HRU33025.1"
                     /translation="MNRDLWERCVAFHGHECPGLAIGFRACEAAKEKLGITFSRDEEV
                     VCVTETDACGVDAIQVITGCTLGKGNLIYRDRGKQAFSFFVRSTGKSIRVVLKPTDKE
                     MTRDERKIYLLETPIDELFEFKKPQFELPEIARIFRSVICEICGESTAEHRVRIMDGK
                     MVCLDCFKDYTRGWEGRSL"
     gene            complement(1419..>2312)
                     /locus_tag="P5310_06400"
     CDS             complement(1419..>2312)
                     /locus_tag="P5310_06400"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRU33026.1"
                     /translation="NIEIILSDVLWDFQILKKFIDGRLDYYKKIRFLFPLPQFHNDVR
                     SFDFVQSVGALLDRHIVSLEDYLEVIYELLKRAKDRGIAGIKDQSAYTRTLDYELVPM
                     YEAEKLFNRILSDPRNSLGWTEAKPLDDFLFHQFMNFARDLKLPVQIHTGHMAGIRNR
                     IEKTNAVLLTNVFELYQDLKFDLFHGNWPYIGEMLFLTKNYPNVYMDLCWVNIIDPIY
                     SEELYSRAILTTPHTKINGFGGDYFDVPEYIVSHLKICRENITRALVRLIKMGWIDEG
                     SALEIAKDWLFNNPKSLFNLS"
BASE COUNT          642 a          578 c          354 g          738 t
ORIGIN      
        1 tcaatcataa tactcccctc cctgcctgtc tcatcaacca cgttcctggc gtttaccatc
       61 acaaatgggg gatatttagc ctgagtgctc tctatcccat gtccaccctt cattcccaga
      121 gctccaacat acacccctgg cttctttcca tcagctattt tttctgtcct taaatctaat
      181 tctctctgtg tcttttttat ataatttaca atatctccag cccttttctc ctctccgatt
      241 atcatgcctg taagtcttaa tgacttatac agagattcat taataatagt ggatattccg
      301 taatctaaca ttaccaccgg aatccctgtc ttgagttgta ggtcttcagc tccttttcta
      361 tctataaacg agcaggcaaa aatcacatcc ggtttcacat tcacaatctt ctccgggtca
      421 ggagaagaga atgccccacc ctgtcctata accggtaatt ttttcaactc tggataagcg
      481 atgaggtagg tctttccccc aggagaaccc tctctatcta tattctctat cccaaccacc
      541 ctgttgactc cgtttacata acagaccagt ctcagtgcac caggtccaat agccactatc
      601 cttctggctg ggataggaac ctcaaccctt cttccagcca gatcagtaac agttattttt
      661 ccagctaaac ttccagaccc aatgttaaca attaaaagga ctgataatag tagaatcgat
      721 actcttatca gtctcacaaa cttcttcctt cccagcccct ggtatagtct ttaaaacaat
      781 ccaaacagac cattttccca tccattatcc tgactctatg ttctgcggta gattctccac
      841 atatttcaca tatcaccgac ctgaagattc tggctatttc tggtaattca aattgtggct
      901 ttttaaattc aaataattca tctatcggtg tttccaagag atatatcttt ctctcatctc
      961 tcgtcatctc tttatctgta ggcttcaata caaccctaat acttttaccg gtagacctta
     1021 caaagaaact gaaagcctgt tttcctctat ctctgtatat taaatttccc ttacctaaag
     1081 tgcagcccgt tattacctgg atggcatcaa ccccacaggc gtcggtctca gttacacata
     1141 ccacctcttc atctctggaa aatgttatcc ctaatttttc ttttgctgcc tcacaggctc
     1201 taaatcctat ggcaagtcca ggacattcat gcccgtgaaa ggcaacacat ctttcccaca
     1261 gatctctatt caagtataat cactcctttc ccgtataaaa atttaagcca cgaaagctat
     1321 tgagccttcg tggcttgttc tttctcccaa taaaatatta attatacaga accttcatgg
     1381 ttcactgtaa taaatatacc atagtaaagt taagagcgtc aagagagatt gaataagctc
     1441 tttggattgt taaacaacca gtctttggct atctctaacg cagaaccttc gtctatccag
     1501 cccatcttta tcagcctaac cagagctctg gtgatatttt ctctgcatat cttcagatgg
     1561 gatactatat attcagggac atcaaaataa tctccaccaa agccatttat ctttgtatga
     1621 ggagttgtaa gaatagccct ggagtataac tcttcactgt atatcgggtc gattatattt
     1681 acccagcaca ggtccatata gacattggga tagttcttgg tcaggaagag catctctcca
     1741 atatatggcc aatttccatg aaacaggtca aactttaaat cctggtacag ctcgaagacg
     1801 ttggtcagca gtactgcatt tgtcttctct atcctgtttc ttataccagc catatgtccg
     1861 gtatgtatct gaactggcag tttaaggtct ctggcaaaat tcataaattg atggaataaa
     1921 aaatcatcca gaggcttagc ctctgtccag cctagagagt tcctgggatc agagagtatc
     1981 ctgttaaaca gtttctctgc ctcatacatt ggaaccagtt catagtccaa agttctggta
     2041 taggcactct ggtcttttat accagctatc cctctatcct tggctctttt caaaagttca
     2101 tagattactt ccagatagtc ttccagtgat acaatatgtc tgtccagtaa agcacccact
     2161 gactgaacaa agtcaaaact tctcacatca ttatgaaact gtggcagagg gaataaaaat
     2221 cttatcttct tataataatc aagccttcca tcgataaatt ttttaagtat ctggaaatcc
     2281 cagagtacat cagagagtat tatctctatg tt
//
LOCUS       DAOTKU010000265         2305 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_37658_length_2305_cov_2.206667, whole genome shotgun sequence.
ACCESSION   DAOTKU010000265 DAOTKU010000000
VERSION     DAOTKU010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37658_length_2305_cov_2.206667"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="P5310_06405"
     CDS             <1..281
                     /locus_tag="P5310_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HRU33027.1"
                     /translation="PYIPDDRWNELDREVRSEPREALLGGKEGLDFYPEIAKVISKNL
                     VREGVFAVEIGGKDQVDKIRQIFKDYNIINVNVENDLNGIPRVIWGRI"
     gene            283..921
                     /locus_tag="P5310_06410"
     CDS             283..921
                     /locus_tag="P5310_06410"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HRU33028.1"
                     /translation="MFISKDEKDIEILAEFIREGKLVAFPTETVYGLGTNASIKESVY
                     RIYEVKGRGEEKPLSLHIGTLSRLEDLIELDPLTKRLLLHFLPGPINIIVRKKKNTLD
                     WIGKGDTLGVRFPSLFISQKFLRMADVPVVATSANLSGREEPKNAEDVIRMIGDKIDA
                     ILDWGPVPLGIPSTVVNILEQDINIIREGPIPREKLEDIIEEIKIKTELEGL"
     gene            918..1367
                     /gene="rpiB"
                     /locus_tag="P5310_06415"
     CDS             918..1367
                     /gene="rpiB"
                     /locus_tag="P5310_06415"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HRU33029.1"
                     /translation="MKVAIGSDHAGFRMKEELKNYIKTLDIEVKDLGCYSEDPVDYPD
                     IARDVAISTVKGETDRGILICGTGIGMSIAANKIKGIRAAVGNEILSVRFSREHNNAN
                     ILTLGARIIGIEVAKEAVRVFLETSFLGGRHQRRIEKIGGMEELRCI"
     gene            1358..>2305
                     /gene="glyA"
                     /locus_tag="P5310_06420"
     CDS             1358..>2305
                     /gene="glyA"
                     /locus_tag="P5310_06420"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014042044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HRU33030.1"
                     /translation="MYLEVSDPEIYNAIKGERMREETTLELIASENFTSLAVLEAQGS
                     LLTNKYAEGYPKKRYYGGCQWVDVVEQLAIERAKTLFKADHANVQPHSGAQANMAVYF
                     AVLNPGDTVMGMNLSHGGHLTHGSPVNISGKYFNFVSYGVNPETETIDYDQVRDIARA
                     NRPKLIVTGASAYPRVINFEIFSQIAKEVDSPLMVDMAHIAGLVAAEIHPSPVPWAEF
                     VTTTTHKTLRGPRSGLILCKADYAKAVDRAIFPGTQGGPLMHIIAAKAVALKEAMTPE
                     FRAYQNQIVKNARTLAERLVENGLRLVSGGTDNHLMLVDV"
BASE COUNT          798 a          376 c          590 g          541 t
ORIGIN      
        1 caccttacat ccctgacgat agatggaacg aactggatag agaagtaaga agtgaaccga
       61 gagaggcgct cctgggagga aaagaggggt tagattttta tcctgagata gcaaaggtca
      121 tatctaaaaa tttagtcagg gaaggagtat ttgcagtaga gataggtgga aaagatcagg
      181 tcgataagat aaggcagata ttcaaggatt ataatataat aaatgtaaac gtagagaatg
      241 acctgaatgg cataccgaga gtaatctggg gtagaatata atatgtttat atcaaaagat
      301 gagaaagata tagaaatttt agctgaattt ataagagagg gaaaacttgt tgctttcccc
      361 acagagaccg tatatggact gggaaccaac gcatctataa aagaaagtgt ctacagaata
      421 tatgaagtaa aaggaagagg agaggaaaaa ccgctaagtc tccatatagg gacactcagc
      481 agattagagg atcttattga attggaccct ctaactaaaa ggttactgct tcatttcttg
      541 cctggaccaa taaatataat agtcaggaag aaaaaaaata ccctggactg gataggaaaa
      601 ggagatacac tgggtgtaag attcccttcc ctctttattt cccagaaatt cctgagaatg
      661 gctgatgtcc ctgtagttgc taccagtgct aatctatctg gaagagaaga accaaaaaat
      721 gctgaagatg tgatcaggat gattggagat aaaattgatg ccatcctgga ctggggtcct
      781 gtccctttag gtataccatc cacagtggta aatattcttg aacaggatat aaacattata
      841 agggaaggtc ccattccaag agaaaaattg gaggatataa tagaagagat aaaaataaag
      901 acagagctgg agggattatg aaagttgcta taggaagtga ccacgctgga tttcggatga
      961 aggaagagct aaaaaattat ataaaaacct tagatataga ggtgaaagat cttggctgtt
     1021 actcagaaga tcctgtggat tatccagata tagcaaggga cgttgccata agtacagtaa
     1081 agggtgagac tgatagaggg atacttatat gtgggacagg gatagggatg agtatagcag
     1141 ccaataaaat caaggggata agagcagcgg ttgggaatga aattctttct gtaagatttt
     1201 caagagagca taataatgcc aatatactca cattaggggc gagaattatc gggattgagg
     1261 tggcaaaaga ggctgtcagg gtattcttgg aaacctcttt tctgggagga aggcaccaga
     1321 gaaggataga aaaaatagga ggaatggagg aattaagatg tatctagaag tgagtgatcc
     1381 tgagatatat aatgctatca aaggggaaag gatgagggaa gaaacaaccc tggagctgat
     1441 agccagtgag aattttacca gcctggcggt actggaagcc cagggttcat tactcaccaa
     1501 taagtatgca gagggttatc caaaaaagag atactacggt ggatgccagt gggttgacgt
     1561 ggtagaacag ttggcaatag aaagggctaa gacgcttttc aaggctgacc atgctaacgt
     1621 tcaaccacat tcaggagctc aggctaatat ggcggtctac tttgcagtcc taaacccggg
     1681 agataccgtc atggggatga atctatccca tggtggacat cttacccacg gaagccctgt
     1741 aaatatatca ggtaaatatt tcaactttgt ctcttacgga gtaaatccag agacagaaac
     1801 tatagactac gatcaggtaa gggatattgc cagggcaaac agaccaaaac ttatagtaac
     1861 cggagccagt gcatatccaa gagttataaa tttcgagata ttctcacaga tagcaaaaga
     1921 ggtggattca ccactgatgg ttgatatggc tcacatcgca ggattagttg ctgcagagat
     1981 tcatccgagt cctgttccat gggcagaatt tgtaaccaca acgacacata aaactctcag
     2041 aggtccaagg agtggactta tactatgtaa ggcagattat gcaaaggcgg tagatagagc
     2101 tatattcccc gggacgcagg gtggacctct tatgcacata atagcggcaa aggcggtggc
     2161 attgaaagaa gctatgactc ctgaattcag agcataccag aaccagatag taaagaatgc
     2221 caggacactg gcagagcgtc tggtagaaaa cggattgagg cttgtctcgg gtggaacaga
     2281 caaccatctg atgctggtag atgta
//
LOCUS       DAOTKU010000266         2300 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_37758_length_2300_cov_4.488641, whole genome shotgun sequence.
ACCESSION   DAOTKU010000266 DAOTKU010000000
VERSION     DAOTKU010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37758_length_2300_cov_4.488641"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(190..753)
                     /locus_tag="P5310_06425"
     CDS             complement(190..753)
                     /locus_tag="P5310_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HRU33031.1"
                     /translation="MSRLYKSIEELLVLKVYRRLRLYIERREFMKRFIVLLFVIVSLL
                     ITTQSIGMDIEIPEFPRGRLRIYQGDRELIIPIEIADKPELWSFGLMYREDIPWNYGM
                     LFVFPSNESGGFWMKNTLVSLDIAFIDSSKTIFNIQRMVPCNDDDCPVYYSPKPYRYA
                     LEVKAGFFEKFGFSEGSGIEILSVSSR"
     gene            1013..>2300
                     /locus_tag="P5310_06430"
     CDS             1013..>2300
                     /locus_tag="P5310_06430"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRU33032.1"
                     /translation="MIKGMILGAGEGTRLRPLTSNHPKPMMPVLNRPIMEHIIRLLKV
                     HNITELWCNLYYLPDEIQNYFEDGSNLGVRISFKVEDKLPGTAGGVKNLEEHFDDTFV
                     VISGDAITDIDLTSAIKFHRERGAIATIVLTHVENPLEFGVVITDEEGRIVRFLEKPG
                     WSEVFSDTVNTGIYILDPKVFDFIPKGQDFDFSKDLFPLLLEEKQPLFGYIADGYWCD
                     VGNIESYMKVHYDALSGKVKLDITGQEISPGIWVGKGVSISSSANIKGPVLIGDNSQI
                     KDAARIMEFTVIGDNTLVDANALLLRSIVWSNSYIGESSTITNAVIGKKAIIKNRVRI
                     GENSVVSDECFVDEGTLIKPGVKVWPSKSIEKGTVLSSSLVWGTSWRRLYFSNGTIFG
                     LANIEINPEFSAKLGSAFGGILERGSEVVSSRDANRS"
BASE COUNT          745 a          412 c          461 g          682 t
ORIGIN      
        1 agcagacttc aaatcaacaa tttcactgtg tattggaagt accgcctcta tttcctgtct
       61 tacaacctct ctggctatcc tatatacatc ttccttttct accgtttcca taacaacctc
      121 caagaaaagt tcatacttat tatattatac tatgagcagt ctatttttta aatctttgac
      181 cttgaaattt tacctggaag atacgctcag tatctcaatt ccacttccct cactgaatcc
      241 aaatttttca aaaaagccag cctttacctc aagagcgtat ctatacggtt ttggactgta
      301 atagacagga caatcatcgt cattacaggg caccattctc tgtatattaa agattgtctt
      361 actggaatct ataaatgcta tatcaagtga taccagtgtg tttttcatcc agaatccgcc
      421 agattcattg gatggaaaga cgaagagcat tccatagttc catggtatat cctccctgta
      481 cattaaacca aaactccaaa gttcaggctt atcagctatc tctatcggta ttataagttc
      541 tctatctccc tgatatatcc ttagccttcc cctgggaaat tcgggaattt caatatccat
      601 tccaatgctc tgggtagtta tgagtaaact cacgataaca aaaagtaaaa caataaacct
      661 tttcataaac tctctccttt caatgtagag tctcagcctc ctgtatacct ttaaaactaa
      721 aagctcttct atagatttat acagcctact caccctttct aattctagca tatttaaatg
      781 gataatgcct gcatttataa agattgtgga gttaaaatgc agtctcatgg cttgggtagg
      841 gaaagggacg tcctcatatg agattaattt gtatcccgta ggggcaattc atgaattgcc
      901 cctacaattt cgtcacgcaa aaatttatag aatcacaaag gaattgacaa gaataaccaa
      961 gagagataaa atattattag agaaaaaatc cctttatgaa agggtgataa ttatgataaa
     1021 aggaatgatt ttaggagcag gagaagggac aaggttaaga ccgttaacat caaatcatcc
     1081 taaacccatg atgcctgttt tgaatcgtcc cataatggaa catatcataa gactcttaaa
     1141 ggttcataat attactgagc tctggtgtaa tttatactat ctccctgatg aaatacaaaa
     1201 ttacttcgag gatggttcta accttggggt ccgcatctct tttaaagtag aagataaatt
     1261 accaggtaca gctggggggg ttaagaatct cgaagaacat tttgatgata catttgtggt
     1321 cataagtggt gatgcgataa cagatataga tcttacatct gccataaaat ttcacaggga
     1381 aagaggagct atagcgacca tagtacttac ccatgtggaa aacccactgg agtttggagt
     1441 tgtaattact gatgaggagg ggcgtatagt cagatttctg gaaaaaccgg gatggagtga
     1501 ggtattcagt gatactgtta atacgggtat atacatcctg gacccaaagg tatttgattt
     1561 tatacctaaa ggacaggatt ttgacttcag taaagacctg ttcccacttt tattggagga
     1621 aaaacaacca ttatttggct atatagcaga cggatactgg tgtgatgttg gaaatataga
     1681 gagttatatg aaggtacatt atgatgccct atcaggtaag gtaaaactcg acataacagg
     1741 acaggagata tctccaggaa tatgggttgg caaaggggta agtatatctt ccagtgcaaa
     1801 tataaagggt cctgttctca taggagataa ctctcagata aaagatgctg ccagaataat
     1861 ggagtttact gtaattggag ataatacact cgtagatgcc aatgcgctat tattgagaag
     1921 tattgtatgg tctaactcat acataggtga atcttctacc attaccaatg cggtgattgg
     1981 taaaaaggcg ataataaaga atagagtgag gataggggag aattctgtag tatcggatga
     2041 atgttttgta gatgaaggta cacttataaa gcccggggta aaggtatggc cttcaaagag
     2101 tattgagaag ggaacagttc tatcatcaag tctagtctgg ggaacgagtt ggagaaggct
     2161 ctacttctcc aatggaacaa tctttggtct ggctaatata gagataaacc ccgaattttc
     2221 tgctaaacta ggaagtgctt ttggtgggat actagaaagg ggatcagaag ttgtatccag
     2281 cagagatgct aacagatcat
//
LOCUS       DAOTKU010000267         2298 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_37800_length_2298_cov_3.469906, whole genome shotgun sequence.
ACCESSION   DAOTKU010000267 DAOTKU010000000
VERSION     DAOTKU010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37800_length_2298_cov_3.469906"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="P5310_06435"
     CDS             complement(<1..220)
                     /locus_tag="P5310_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HRU33033.1"
                     /translation="MEDKSSIIYIIETLRPRQWVKNFLIFAGLIFSQNLFNPEKFLYS
                     LLAFLLFCLVSGIVYTVNDIIDLPYDKVN"
     gene            complement(248..646)
                     /locus_tag="P5310_06440"
     CDS             complement(248..646)
                     /locus_tag="P5310_06440"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRU33034.1"
                     /translation="MSIKGLDHISIIVSDLEENLNFYRDTLGFSIVRDFYDEKEKARI
                     VFLNIGNTMLELIAPDDRPSEGMKTSWEERKRGGVEHIALLVDNMDDTYNYLKSKGID
                     FILGPIEFPDGKYGYFYGPDGVLLEVICWK"
     gene            complement(666..1775)
                     /locus_tag="P5310_06445"
     CDS             complement(666..1775)
                     /locus_tag="P5310_06445"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRU33035.1"
                     /translation="MRLEFNPEKYQTQIEESRRRLEIARSFKEPDRVPITISTGGSFY
                     SRLFGFNIRDYYTNLDVQIEVQVKGLKWAFEELQDDRVGYGVNLDLGPVYEGIYFDLP
                     IEYPDDTSPWVVRTMSLPEAVERLKTPDPEDHPGIIDFYKKYELLKEKVSKLGLNIPV
                     GGRFGIHPPLSAACGILEPAEVFMYLVEDPQLVHRLFEKLLIAFIKIHDFGDKYFGVK
                     TTHLGLADDHSAFVSDEMYRQHIMPYNMLLYGRYGINGRHLHADGPNDHHFKTYADIV
                     KLTSMDIGGFSDIANAKPVFKDKVFFSGGLNCKDLYYDLETAEKAVRRAISIGAPGGG
                     YALAVGGETYAGVKPDTLIQMVKYAKEIGKYPIKI"
     gene            complement(1759..>2298)
                     /locus_tag="P5310_06450"
     CDS             complement(1759..>2298)
                     /locus_tag="P5310_06450"
                     /inference="COORDINATES: protein motif:HMM:NF016972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRU33036.1"
                     /translation="RVSEEWKELYPLAERIILEEMGRLKSMGVGVSIHIIGEVSLIRN
                     MVAVRFSSIEEAKNYEKQLREKLKSSGVPLWSNYNFSLVQILPPSAGKGNTVLTLSRH
                     LGLLPEEVLVIGDSGNDKDMLDGRFGFRVATVSNAEPEIKETVERNGGYVAENPVSRG
                     IIEIVNKLILGEIKYETGI"
BASE COUNT          676 a          561 c          341 g          720 t
ORIGIN      
        1 gattaacttt atcatatggt aaatctatta tgtcatttac agtataaact ataccagaga
       61 ccagacagaa gaggagaaag gctaaaaggg agtataaaaa cttctccgga ttaaagaggt
      121 tctgagagaa tattaaacca gcaaaaatta aaaagttctt aacccattgc ctgggacgaa
      181 gggtctctat aatataaatt atactggact tatcttccat cagtagtcta gggaaataat
      241 caccgatcta cttccagcaa atcacttcca gtagaacccc atcaggtcca taaaaatatc
      301 catacttccc atctggaaat tctattggac ccaggataaa gtcaatccct ttcgacttta
      361 aatagttata agtatcatcc atattatcaa ccagaagggc aatatgttcc acccctcctc
      421 tctttctctc ctcccaggaa gttttcatcc cctctgatgg tctatcatcc ggggctatta
      481 actccagcat agtattaccg atattgagaa atactatcct cgccttctct ttctcatcat
      541 agaaatccct tactatacta aaaccgagtg tatctctgta aaaattaaga ttctcttcaa
      601 gatcactaac tataatggat atatggtcta atccctttat agacataaaa acctcctcta
      661 taaatttata tttttattgg atatttgcca atctccttgg catatttaac catctgaatg
      721 agtgtatctg gttttacccc tgcatatgtc tctcctccaa ccgcaagggc ataaccacct
      781 cctggggctc caatacttat agcccgtctt acagcctttt cagctgtctc caaatcataa
      841 tacaggtctt tacagttcaa tcctccactg aagaatactt tatccttgaa tactggcttt
      901 gcattagcga tatcgctgaa tccacctata tccatagatg ttaacttcac tatatctgca
      961 taggtcttaa aatgatggtc gttaggtcca tctgcatgaa gatgtctccc attaattcca
     1021 taacgaccat agagaagcat attatatggc attatatgct gtctatacat ctcatcagag
     1081 acaaatgctg agtggtcatc ggcaagaccc agatgggttg tctttactcc aaagtactta
     1141 tctccaaaat catgtatctt tataaatgct atcagcagtt tttcaaaaag cctatgcact
     1201 aactgtggat cttctacaag atacataaag acctcagctg gttccaatat gccacaggct
     1261 gcagataggg gtggatgaat cccgaatctt ccaccaacag ggatattaag cccgagtttt
     1321 gatactttct cctttaaaag ttcgtacttc ttgtagaaat ctatgatgcc aggatggtct
     1381 tccgggtcag gagttttcag cctttctacc gcttccggaa gactcatggt cctcaccacc
     1441 cagggagacg tgtcatcagg atattctata ggaagatcaa aatatatacc ctcataaaca
     1501 ggtcctaaat caaggttaac accatatcca accctatcat cctgcaattc ttcaaatgcc
     1561 cacttcaatc ccttcacctg aacctcgatc tgaacatcca gattggtgta ataatctctt
     1621 atattaaaac caaacaacct ggaatagaaa cttccacctg tacttatagt gattgggacc
     1681 ctatccggtt ctttaaagct cctggctatt tcaagccttc ttctggactc ttcaatctga
     1741 gtctgatact tttctggatt aaattccagt ctcatatttt atctctccca gtataagttt
     1801 atttacaatc tctattatac ctctacttac aggattttcc gctacatacc caccatttct
     1861 ttcaacagtt tcttttatct ctggctcggc gttagatact gtagcaaccc gaaatccaaa
     1921 tctcccatca agcatatcct tatcattccc cgaatctcca ataaccagaa cctcttctgg
     1981 tagtaatcca agatgcctag atagagttaa gactgtattc cccttacctg ccgatggagg
     2041 aagtatctgg accagactaa aattataatt actccagaga ggcacccctg aggattttag
     2101 tttttctctt aactgtttct cgtaattctt tgcctcctct atactgctaa atctcacagc
     2161 caccatattt ctaatcaggg agacctctcc tattatatgg atagaaactc ctacacccat
     2221 actctttagt ctacccatct cctctaaaat tattctttct gctaaaggat agagttcttt
     2281 ccactcttcg gataccct
//
LOCUS       DAOTKU010000268         2295 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_37862_length_2295_cov_4.082589, whole genome shotgun sequence.
ACCESSION   DAOTKU010000268 DAOTKU010000000
VERSION     DAOTKU010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37862_length_2295_cov_4.082589"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(138..1403)
                     /locus_tag="P5310_06455"
     CDS             complement(138..1403)
                     /locus_tag="P5310_06455"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRU33037.1"
                     /translation="MSNTGLSPRENRRNFILGATQSGLWGLGLTFSSINTVIPMFLKK
                     LGASDVEIGLIPTLANLGFTLPSILSAPIVERVKSLKKFITIVTWIERAPYLFLAILA
                     FTLAKHNPILTIYLSLVFVGIGNFGTGFIMPAWMDFIARIIPVTSRGKFTALGNGLGG
                     LLGIAGSMMVGKILNSHPFPTNFGLCFTITIAILIMAYVVLLFMKEAPNKKDQRREKI
                     NYSYDIAKILRSDRHFRNYVLARFIVSFGSMGLAFYTVYAVGRFSLSDEYIGTFTMFF
                     VGGQPLANLIWGPLADKTGHRNVLLGGIFCNLLGNLIAIITPSVYPLYLVFALSGAMN
                     SSFMISGLPIILEFAPPDRRPTYIGINNMIISPALALSPLIGGIVAENFGYQYVFRIS
                     LIANIIGLTILYLGVIDPRKRQIHTEIEG"
     gene            complement(1396..2196)
                     /locus_tag="P5310_06460"
     CDS             complement(1396..2196)
                     /locus_tag="P5310_06460"
                     /inference="COORDINATES: protein motif:HMM:NF019413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1638 domain-containing protein"
                     /protein_id="HRU33038.1"
                     /translation="MKPQEGLTELTRLIQAQKKKIAVVACEVLAREICYAGLITSNIV
                     HLVFKTKGLHDNPDKMREELQEDIDKISFSSSEEYQAIVLAYGLCSNGTMGLIARNIP
                     LIIPKAHDCITLFLGSKERYAEEFSKNPGTYYFTTGWVERGSESVGRTSASGYGLGKT
                     YEEYVELYGEDNARYLFELEQSWLIRYNLATYIDMKLPMPFSYEDDVKKEAERRGWKY
                     KKIEGDMKLILDSINGNWDEEDFLIVPPGYSIQPSYDGNIIKIEPADE"
BASE COUNT          684 a          480 c          435 g          696 t
ORIGIN      
        1 aaaataatct cagaaatgta cctgaggttg gattctttac tatggcatgg cctttgagtg
       61 ctaatccaga gcagtttttc tttactaaga gccagaaata acaactaata gtagagatag
      121 aggggataaa ggacctctta cccctctatc tctgtatgaa tttgtctctt ccttggatct
      181 ataacaccca gatagagtat agttaaccct atgatgttgg ctataagcga aatccgaaaa
      241 acatactgat atccaaaatt ttctgctact atccctccta tgagagggct taaggcaagg
      301 gcaggggaga tgatcatatt gtttatgcct atatatgtgg gtcttctatc tggaggggca
      361 aattctaata ttataggaag tccagaaatc ataaaggagc tattcatcgc tccagatagt
      421 gcaaagacga gataaagcgg gtatacagac ggtgtaatta tggctataag attaccgagg
      481 agattacaga aaatacctcc taacaggaca ttcctgtgcc ctgtcttatc tgccagagga
      541 ccccagataa ggttggctaa cggttgacct ccaacaaaga acatggtaaa agttcctatg
      601 tattcatcac tcaatgaaaa tctgccgacc gcatatactg tgtaaaaagc tagacccatc
      661 gacccaaaac ttacgataaa tctggccaga acataatttc tgaaatgtct gtcagatctg
      721 agaatctttg ctatatcata ggaataattt atcttttctc ttctctggtc ttttttgttt
      781 ggagcttctt tcataaataa cagtactaca taagccataa ttaatatggc tatggtgata
      841 gtaaagcata gaccaaaatt cgtcgggaat ggatgtgagt tcaggatttt cccaaccatc
      901 atagaaccgg caattcccag taatccaccc agcccattcc ccagggctgt gaatttccct
      961 cgactggtga caggtataat tctggcaata aaatccatcc aagcaggcat tataaaacct
     1021 gtaccaaagt tacctatacc tacaaatacc agtgagagat aaatagtcag gatggggttg
     1081 tgttttgcta aagtaaaagc cagtatggca agaaaaagat atggagccct ttctatccat
     1141 gtaactatag ttataaattt ttttaaggat tttactcttt ctactatagg ggcagataaa
     1201 atgcttggaa gggtaaaacc aagattggcg agcgtgggta taagacctat ctccacatcg
     1261 gatgctccta attttttcag gaacataggt atgactgtat tgatagaaga aaaagttaga
     1321 cccaagcccc ataaacctga ctgggtagca ccgaggataa aatttcgtct attttctctc
     1381 ggagataaac cggtattact catccgcagg ttctattttt atgatatttc catcatagct
     1441 gggttgtata gaatatcctg gaggaacgat caaaaaatct tcttcatccc agtttccatt
     1501 tatagagtca aggataagtt tcatatcccc ttctattttt ttatacttcc aacctcttct
     1561 ttccgcttct tttttcacat cgtcttcata cgaaaatggc attggcagtt tcatatctat
     1621 ataggtagcc agattataac gtataagcca ggattgttcc agctcgaata agtatctggc
     1681 attgtcttcc ccatatagtt ctacatattc ttcataggtc ttccccagtc cataaccgct
     1741 ggcggatgtc cttcccacac tttcagaacc tctctcaacc caaccggtgg tgaaatagta
     1801 tgtgccagga ttcttagaaa attcctccgc atacctctct ttagagccaa gaaaaagcgt
     1861 aatacagtca tgagcctttg gaataatcag aggaatgttt ctggctataa gtcccatagt
     1921 accattgctg cagagcccat aggctaatac gatagcctga tattcttcag aagaagagaa
     1981 agatatctta tctatatctt cctgaagctc ttctctcatc ttgtcgggat tatcgtggag
     2041 tcccttagtt ttgaagacaa gatgaactat attgctggtt attaaaccag cataacaaat
     2101 ctctctagca agaacttcac aggcaactac tgcaatcttt tttttctgcg cctggatgag
     2161 ccttgtaagt tcagtaagtc cttcctgggg cttcatttaa tctcctccag tgtatctttt
     2221 atggcaaaga gactttctct ggatgtttct ccagggacag cacatccagg ggttactatg
     2281 agttttttag ctcct
//
LOCUS       DAOTKU010000269         2293 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_37916_length_2293_cov_2.503128, whole genome shotgun sequence.
ACCESSION   DAOTKU010000269 DAOTKU010000000
VERSION     DAOTKU010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37916_length_2293_cov_2.503128"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1524)
                     /locus_tag="P5310_06465"
     CDS             complement(<1..1524)
                     /locus_tag="P5310_06465"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRU33039.1"
                     /translation="MKLVRVLLLILICMSLVLSSVSSIFAEVVSYPKQYNSPAEYTKV
                     TGKKIAKFGEAPMLAELVKQGKLPPVNRRLPGEPLVIEPTKEIGQYGGTVGIIHTNPQ
                     NFEDEVNIGGKESILTFDPQDGATILPNLAKSWIFSKDGKTLILELRKGIKWSDGQPF
                     TADDIEFWWKDVVLNDELTPAKPKVWAPGGKLMKLEKLGPYSVRLQFAISYPAALIQL
                     AAWGTEGSFFLPKHYLRQFHIKYNPQANELAKKEGFDSWIRLFQSKSVVGYLDGIQNP
                     EAPVIRAYKIVSRTPETTILERNPYYWKVDTSGNQLPYIDRVRLTLVTNVEVYTTKVV
                     SGEVDLAQWNTTLDNYTLYKENEKKGGYRVINYQTAWPSMAEIFLNLNHKDPVVRKIF
                     NDLRFRKALSLAINREEINQMVFHGMAEPLQTVILPRGGRFWDERLAKLYTEYDPKEA
                     NRLLDEMGLDKKDKDGFRLRPDGKPLQITIDYWPGEGGPAKISIVELVENYWSKVGVK
                     "
     gene            complement(1556..>2293)
                     /locus_tag="P5310_06470"
     CDS             complement(1556..>2293)
                     /locus_tag="P5310_06470"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HRU33040.1"
                     /translation="ERGLKILELLEKEKEGLTFGDLLEKTGIPKPSLARILNLLESHN
                     FIRNSSDGRYRLGFRLLSLGYSVYTNLDLIRESRPFLTELVDLTGETVELAILDGGEI
                     LYIDKIESPEPIRLVARIGSRYKTLHCTAVGKVFLAYMGEDFFKDYLARVGLPSFTEK
                     TITDVEVLKAQLDEIRSRGYAFDDEEVRLGVRRIASPVFGSFKGLVAVIGIAGPTFRI
                     TVERKEELGEIVKRVAMGLSERFGYKE"
BASE COUNT          626 a          579 c          353 g          735 t
ORIGIN      
        1 ttttacacct accttactcc agtagttctc taccagctca actatagaga tcttggctgg
       61 acctccttct cctggccagt aatcaattgt gatctgaagg ggtttcccat caggtcttag
      121 tctaaaacca tctttatctt tcttatccag ccccatctca tcaagtaatc tattcgcctc
      181 tttagggtca tattctgtgt agagtttcgc caatctttca tcccagaatc ttccacctct
      241 cggcaggata actgtctgca gtggctcagc cattccatgg aataccatct ggtttatctc
      301 ttctctattt atagccaaag atagggcttt tctaaatcta aggtcattaa atatcttacg
      361 aacaactgga tctttgtgat tcagattcaa gaatatttct gccatagatg gccaggctgt
      421 ctggtaatta attaccctat aaccaccctt cttttcgttc tctttataaa gagtatagtt
      481 atctaaagta gtattccatt gcgccagatc cacctctcca cttacaacct tggtggtata
      541 aacctcaacg tttgtaacca gtgtcaacct gactctgtct atatagggca attgattacc
      601 tgaggtatct accttccaat aataagggtt tctttcaagt atggtcgtct caggcgtcct
      661 gcttactatc ttatatgccc ttatcacggg tgcttcaggg ttttgtatgc catccagata
      721 tcccactaca gatttagatt gaaatagtct tatccaggag tcaaaccctt cttttttagc
      781 taactcatta gcctgtggat tatacttaat atggaactgc ctgagataat gcttcggtaa
      841 gaagaagcta ccctctgtcc cccaggctgc taattgtatc agggcagctg gataagatat
      901 agcaaactga aggcgaacac tataaggacc tagcttctct aatttcatca actttccacc
      961 aggtgcccat acctttggct ttgcaggtgt aagctcatca tttaaaacta catccttcca
     1021 ccagaattct atatcatcag cagtaaaagg ttgaccatct gaccatttta tgcccttcct
     1081 tagctctaat ataagcgttt tgccatcttt agagaatatc caggacttag ccagattagg
     1141 taaaattgtt gcgccatctt gcggatcaaa tgtcagaata gactctttac ctccaatgtt
     1201 tacctcgtct tcaaaatttt gaggatttgt atgaattatc ccaactgtcc caccatactg
     1261 accaatctct ttagttggct ctataaccag aggttcacct ggaagtcttc tgttaaccgg
     1321 aggtaacttc ccctgtttta caagttcagc taacataggt gcttcaccaa atttagcaat
     1381 ctttttacca gtaactttag tatactcagc agggctatta tactgttttg gatagctaac
     1441 cacctcggca aaaatagaag aaactgagct taaaactaaa ctcatacaga ttaagattaa
     1501 caacagcact cttactaact tcatttcaat caaccctcct attattaaat taatatcatt
     1561 ctttatatcc aaatctctct gacaacccca tcgctaccct tttaactatc tctccaagct
     1621 cttcctttct ctccactgtt attctgaatg tcggtcctgc tatccctatc acagccacta
     1681 accccttaaa cgaaccaaat acaggagaag ctatacgtct tactcctaat ctcacctcct
     1741 cgtcatcaaa ggcatagccc cttgagcgaa tctcatctag ctgtgccttt aggacttcca
     1801 catcagttat cgtcttctct gtaaaagaag gaagtccaac cctagccaag tagtccttaa
     1861 aaaaatcctc acccatatag gctaggaaaa ccttccctac cgctgtgcag tgtagtgtct
     1921 tatatctgct tccaatacgt gccacaagcc ttatcggctc agggctctct atcttatcta
     1981 tataaagaat ctctcctcca tccagtattg ctaactctac cgtttctccg gtaaggtcca
     2041 ccagttctgt caaaaatggt ctggattctc ttatcagatc cagatttgta tacacactgt
     2101 accccaaaga caacagtcta aagccaagtc tatatctacc gtcagatgaa tttcttataa
     2161 aattgtggga ttcgagcaga tttaatattc tagccagaga aggtttagga attccggttt
     2221 tctctaataa atccccaaaa gtcaaacctt ctttctcttt ttccagtaac tccagtatct
     2281 ttagacctct ctc
//
LOCUS       DAOTKU010000270         2289 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_37991_length_2289_cov_3.366607, whole genome shotgun sequence.
ACCESSION   DAOTKU010000270 DAOTKU010000000
VERSION     DAOTKU010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37991_length_2289_cov_3.366607"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            368..1189
                     /locus_tag="P5310_06475"
     CDS             368..1189
                     /locus_tag="P5310_06475"
                     /inference="COORDINATES: protein motif:HMM:NF012669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HRU33041.1"
                     /translation="MDSIAKINRKRKILDLLEARGTLSINELAEHLGISPITLRKDLA
                     DLERQRLIVRVWGGVSLSTQTTIQEPPFEDRSVENLEEKRAIAKVAVTLVKPNDVIAL
                     GGGTTTYEFAKLLGGIPNVQVVTNSVNIAYLLLRLGVRIAMPGGFSREGSYSLFGDRA
                     EDFFKGIFIDKCFMGVDGIDTEGGLTTLNPSEASIYTSMINSAHTLIVLADHTKLGQK
                     KLIPMSPIDKVDILITDELASKDILKKLEKKGVKIEIAPLESQLESYSHSDYKFK"
     gene            1199..>2289
                     /locus_tag="P5310_06480"
     CDS             1199..>2289
                     /locus_tag="P5310_06480"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HRU33042.1"
                     /translation="MRKNITLNGIWELSLDRRFNPAQTYSIEVPSCIGNQIPALREYR
                     GIVWYRKDFKVDKDRENRYLLHFGAVNYYAEVWLNDELVGSHEGGYTPFTFDVTQNIK
                     SRNKLIVKVIIPGDADPNYPFTEIPHGKQEAQWYGMAGGIWQGVTLIETGSSYIKKMH
                     ITPDLDNSKVYILINSESDGKEEYNLQISLLDPSGKEINRVEAVLEEENRIELNIKNP
                     QVWDVDNPNLYTVRANITYKNKILDEVIETFGMRKIEAKDEELLLNGKPVYLIGALDQ
                     DFYPFTHYNPPDEEFVRDQLILAKEMGLNCLRYHIKVVHPWYLKWADRLGILIWEDLP
                     NWSKSTPSAKARGEKLLEEMVDYDYNHPSV"
BASE COUNT          771 a          368 c          522 g          628 t
ORIGIN      
        1 tactataggg aaatctccct ctgaagcctt aaagattgct gttatgcttg aagatattgc
       61 aaaaactgtt catcttgctc tccttagggg aaaacctcaa gaaataccac ctgaagaggt
      121 acagagagct tatgagtggc atcagaaatt ttatggacag aaataagaca gctggaagtt
      181 actgtgattt aggataaatt cgtcggctga gtttgaaagt gagctcagcc ttatttttta
      241 acctcttaat atcaaattta gagcactttt tatatttttg taaatcaata ttgtttaaac
      301 tattgacata aaaatactat atgatataat ctgttcgtat caaatttata gctagaggtt
      361 aaatattgtg gattcaatag ccaaaataaa tagaaagagg aaaatattgg atctgctcga
      421 agctagaggg acattatcta taaatgagct ggctgaacat cttggaattt ctccaatcac
      481 cctcagaaag gacctcgctg accttgaaag acagagactt atcgtcaggg tctggggagg
      541 cgtttcactg tctacccaga caactatcca ggaaccaccc tttgaggatc gaagtgtgga
      601 aaatctggaa gagaaaaggg ctatagctaa ggttgctgta accttggtaa aaccaaacga
      661 tgtaatagct ctgggtggtg gaaccaccac atatgagttt gcaaagttac tgggggggat
      721 tccaaatgta caggtagtta caaactctgt aaatatagcc tatctgctac tcagacttgg
      781 tgtaagaata gctatgcccg gaggtttttc cagagagggc tcttacagcc tgtttggtga
      841 cagagcggag gattttttca agggtatttt tatagataaa tgttttatgg gagtggatgg
      901 aatagataca gaagggggat taacaacact gaatcccagt gaggcaagta tatatacatc
      961 catgataaac tctgcccata cattaatagt tctggctgat catacaaagc tggggcagaa
     1021 aaaactgatt cctatgagtc caatagacaa ggttgacata cttattacag atgaattggc
     1081 ttcaaaagat attttaaaga agctagaaaa aaaaggtgta aagattgaga tagcaccatt
     1141 ggaatctcaa ttagaaagtt atagccatag tgattataaa ttcaaataag gaggaccagt
     1201 gagaaaaaat ataactctta acggaatatg ggaattgagt ctggatagaa ggtttaatcc
     1261 tgctcagaca tattcaatag aggtaccaag ctgtataggt aatcagatcc ctgctttacg
     1321 agaatacagg ggaatagtat ggtacagaaa ggattttaaa gtagataagg atagagagaa
     1381 tagatactta ctccattttg gagcagttaa ctactatgca gaggtctggc tcaatgatga
     1441 gctggtagga agtcatgagg gtggttatac tccatttaca tttgatgtga cccagaatat
     1501 aaaatctagg aataaactta tagttaaagt aataataccc ggtgacgcag accccaacta
     1561 tccgtttact gagatcccac atggtaaaca ggaagctcaa tggtatggga tggcgggagg
     1621 catatggcag ggtgtaacct taattgaaac aggttcttct tatataaaga agatgcatat
     1681 aacccctgat ctggataaca gtaaggttta tatcctgata aactctgagt cagatggaaa
     1741 agaggagtat aatctgcaga tttcccttct tgacccatcg ggtaaagaga taaacagagt
     1801 agaggctgtg ctggaagagg agaaccgtat agagctaaat ataaagaatc cgcaagtttg
     1861 ggatgtggat aacccgaatc tgtacactgt tagggcaaac ataacttata aaaataagat
     1921 actggatgaa gttatagaaa cctttggtat gagaaagata gaggcgaaag atgaagagtt
     1981 acttctaaac ggtaagccgg tgtaccttat tggtgctttg gaccaggatt tttatccatt
     2041 tacccattat aatcctccag acgaagaatt tgtaagagac cagcttatac ttgccaaaga
     2101 gatgggtctt aactgtttga ggtatcacat taaggtggtt catccctggt atctgaaatg
     2161 ggcggacaga ttgggcattc tgatctggga agaccttcct aactggagca agtctacccc
     2221 aagtgctaag gctagaggag aaaagctatt agaagagatg gttgattatg actataacca
     2281 tccatctgt
//
LOCUS       DAOTKU010000271         2287 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_38028_length_2287_cov_3.772849, whole genome shotgun sequence.
ACCESSION   DAOTKU010000271 DAOTKU010000000
VERSION     DAOTKU010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38028_length_2287_cov_3.772849"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1076)
                     /locus_tag="P5310_06485"
     CDS             complement(<1..1076)
                     /locus_tag="P5310_06485"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRU33043.1"
                     /translation="MKVAVIGNGVSSVNTASNLRSLDNDIEIDIFTDEHYHYYPRPRL
                     IDLLADKVILEEIFFYKESWYEEHNINVHLNSSVKELRPEEKEISLFNGERIKYDFLV
                     LANGAVPSLPPIRGIDKRGVFSIRWLDDVLNLKERVKRSGEVIAIGGGLLGLEIAAAL
                     QSSGVKTKVIEILPWLLPRQLDEVGGNLLKKILEDRGLEIFTGTPTDEISGDIEVSGV
                     KTRDGRVFKGDTVVITAGIKSSTDLAKNGGLNVNRGVVVDSFLRTSVENIYALGDVAE
                     WNGMVYGIIPPALDQAKIVASNILGENKEYRGTVPSNILKVAGIDLFSIGMVNPDTLC
                     EVFGYINEGKNLYKKFVIKNNKLIG"
     gene            1183..2250
                     /locus_tag="P5310_06490"
     CDS             1183..2250
                     /locus_tag="P5310_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF763 domain-containing protein"
                     /protein_id="HRU33044.1"
                     /translation="MERRLAELPLHNGKAPGWLFQRMKRLAREIISLFLIEGKTDELI
                     RNLADPYWFQSLGCVLGFDWHSSGLTTTTGAALKEGLRELSPESGLFIVGGKGKTALN
                     TPEEIKRLADKSGFDADRWINISRLVARVDNNAVQDGYQLYFHLLVFTEDGRWGIVQQ
                     GMNTDNRYARRYHWLGEEVKSFVNEPHKAICTERFEDIVLNLTARESGDTRDIITRLS
                     QQTPDKTVKEWENVILHLPQHHPVDIKEIQPKNLRSILLETYDNPPEDFQELLLHKGV
                     GAKTLRALSLISELVYNQPPSFRDPARFSFAHGGKDGYPYPVDKKLYDTSIDILEKAV
                     KKAKLGQREELNALKRLSLFY"
BASE COUNT          690 a          499 c          448 g          650 t
ORIGIN      
        1 cctataagtt tattgttctt tataacaaat tttttataca gatttttccc ttcatttata
       61 tagccaaaaa cctcacataa tgtatccgga tttaccattc ctatagaaaa tagatctatc
      121 cctgccacct tcaatatatt ggaagggaca gttcccctgt actccttatt ttcccccagt
      181 atattactgg caactatctt agcctggtcc agagccggag ggattattcc atataccatc
      241 ccattccact ccgccacatc tcccagcgca tatatattct ctaccgatgt cctgagaaaa
      301 ctatctacaa ccacccctct gttcacatta agcccaccat tttttgccag atctgtactg
      361 ctctttatac ctgccgttat aacaacggta tcccccttaa aaaccctccc atctctggtc
      421 tttacacctg aaacctctat atccccggag atctcatctg ttggagtacc ggtgaatatc
      481 tccaaccccc tatcttccag gattttttta aggaggttcc cgccaacctc gtccagctgt
      541 cggggtaaaa gccagggaag aatctctata acttttgtct tgacaccaga gctctgtaat
      601 gctgctgcta tctcaagtcc gaggagtcct cctcctattg caatgacctc tcccgacctt
      661 tttaccctct ctttaaggtt taaaacatca tccagccagc gtatactgaa gacacctctt
      721 ttatcaatac cccttatagg aggaagagaa ggaaccgctc cattagccag gaccagaaaa
      781 tcatatttaa tcctctctcc attaaaaaga gatatctctt tctcttcagg gcgtagctct
      841 tttacagatg aattcagatg gacatttata ttatgttctt cataccagct ctccttataa
      901 aagaatatct cctcaagaat aactttatct gccaggaggt ctataagtct gggacgcggg
      961 tagtaatggt aatgttcatc tgtaaatata tctatctcaa tatcattatc cagtgatctc
     1021 aaattactgg ctgtatttac actagagaca ccatttccta taactgctac tttcatctta
     1081 aaaccatctc cttaaacata ggtttttgtt tctaataata tcacaaaaga taccgatctg
     1141 tgttaaaata atctctaatg aaatcaatcg ggtgggggct ttatggaaag aagattagca
     1201 gaattaccac tacataatgg gaaggctcct ggatggctgt tccagcggat gaagagactt
     1261 gccagggaga taatatccct ttttcttata gagggaaaaa cagatgagct tataaggaat
     1321 ctggcagacc catactggtt tcagtccctg ggatgtgtac ttggctttga ctggcattcc
     1381 agtggactta caaccaccac cggagctgcc ctcaaggaag ggttaagaga attatcacct
     1441 gaatcaggac tattcatagt tggggggaag ggtaaaacag cattaaatac cccggaagag
     1501 ataaaaagac tggctgataa atcggggttt gatgctgata gatggataaa tataagcaga
     1561 ctggtggcaa gggtggataa caatgcagtg caggatggat accagctcta cttccatctt
     1621 ctagtattca ccgaagatgg aaggtggggc atagtacagc agggtatgaa tacggataac
     1681 agatatgcca ggagatacca ctggcttgga gaagaggtaa aaagttttgt aaatgaacca
     1741 cataaggcta tatgtacaga gagatttgag gatattgtcc ttaatcttac cgcaagagag
     1801 agcggggata caagagacat cataaccaga ctgagtcagc agacaccaga taaaactgtt
     1861 aaagagtggg agaatgttat tcttcatctc cctcagcatc atccggtaga tattaaagag
     1921 atacagccca aaaatctgcg ttctattctt cttgagacat atgataatcc accagaggat
     1981 tttcaggaac ttctcctcca taaaggtgtt ggagcaaaga ctctgagagc attgtctctc
     2041 ataagtgagt tggtatataa tcagcctccc agtttcagag atccggcaag gttcagtttt
     2101 gctcatgggg gtaaggacgg atatccttac ccggttgata aaaaactgta tgatacatct
     2161 atagatatcc ttgagaaggc tgtaaagaaa gcaaaactgg gtcagagaga agagctgaat
     2221 gccttaaaaa ggctatccct attttactga tggaggtaat aatgaacagg gttatttttg
     2281 aaaaaga
//
LOCUS       DAOTKU010000272         2285 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_38095_length_2285_cov_2.391031, whole genome shotgun sequence.
ACCESSION   DAOTKU010000272 DAOTKU010000000
VERSION     DAOTKU010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38095_length_2285_cov_2.391031"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1026
                     /locus_tag="P5310_06495"
     CDS             <1..1026
                     /locus_tag="P5310_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016483445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HRU33045.1"
                     /translation="VDTSNEIGGDGDIPHPGIGRARRMQVSSPERQHDVMIEAVENHM
                     PEVIVIDEIGTELEAKAARTIAERGVQLIGTAHGITLENILLNPTLSDVVGGIQAVTL
                     SDEEAKKRGTQKTVLERKAPPTFDIVIEIRDRDTLAIHHDVAQVIDYMLKGYDPRPEI
                     RVRRGNTYEVVEEETIPIEEDIALNGPEGDHHLKVYPFGISRNRLEKAIRELNIPALI
                     TKRLEEADLILTLTSHGKREGNKLIAKAHRDIPIYTIKSNTLIQIKNFLNRYFLNEGE
                     EDSEYDRLLAEIDEGIRKAIETLEPVELSPQNARVRRIQHQIIEKYQLHSDSVGEEPY
                     RRVRILP"
     gene            1026..1355
                     /gene="tmk"
                     /locus_tag="P5310_06500"
     CDS             1026..1355
                     /gene="tmk"
                     /locus_tag="P5310_06500"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HRU33046.1"
                     /translation="MSFFTLEGIDGSGKSTVSSIIQKELKKRNANFIAVREPGSTGIG
                     EKIRELLLDPDAFISPIEEVYLYATSRAVFVRRIVLPALNSGKVVISERYVDSSFAYQ
                     GYGKGVP"
     gene            1385..1633
                     /locus_tag="P5310_06505"
     CDS             1385..1633
                     /locus_tag="P5310_06505"
                     /inference="COORDINATES: protein motif:HMM:NF014299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU33047.1"
                     /translation="MSGVSPNLTVLLDIDPSTALKRLEGKKKDRLELEGLDFYERVRD
                     GYLKLAELFRYRYMVVDGTKEPGEIASIVLDKLVRFMQ"
     gene            1630..>2285
                     /locus_tag="P5310_06510"
     CDS             1630..>2285
                     /locus_tag="P5310_06510"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HRU33048.1"
                     /translation="MNLGTLIRLVSVIAGSIVLWYLSNSIGNYLILKIGNLQYRGDKQ
                     RIITLGSLITWIIRVIIGVTAVTWVLRLLNIDPSPFIAGAGILGLAVSFGAQNLIRDT
                     INGFFIILENQYDIGDEVKIGAIQGTVESLTLRITRVRDDSGALYIVPNSTISQVANL
                     SDRWSRILYTLNVSNKEPIEKLERVISYAKDKLQDKYDKELIEGPRIDGIQAFDAATL
                     "
BASE COUNT          772 a          354 c          572 g          587 t
ORIGIN      
        1 gtagatacat ctaacgagat aggtggagat ggcgatatac ctcatcccgg tataggaaga
       61 gccaggagga tgcaggtctc ttctcctgaa aggcagcatg atgtgatgat agaggcagta
      121 gagaatcata tgccagaggt tatagtgata gatgagatag ggacagaatt ggaggctaaa
      181 gcagccagaa ctatagcgga gagaggggtt cagctgatag gtaccgctca tggaataact
      241 cttgagaata tactgctgaa tccaacactt tccgatgtgg ttgggggtat ccaggcagta
      301 acattaagtg atgaagaagc caaaaagagg ggaactcaga agacagtcct ggagaggaaa
      361 gctcccccaa ccttcgatat agttatagag ataagggata gagataccct tgccatccat
      421 catgatgtgg ctcaggttat agactatatg ttgaagggtt atgaccccag acctgagatc
      481 agagtgagaa ggggaaatac ttatgaggtt gtggaagaag agaccatacc gatagaagag
      541 gatatagcct taaatggacc tgagggagat catcacctta aggtttatcc atttggtata
      601 agcagaaata gactggaaaa agcaataaga gaacttaata taccagctct tattaccaag
      661 aggctggaag aggcagactt aatcctcacg ctcacctccc atgggaaaag agagggtaat
      721 aagttgatag ccaaagctca tagagatata ccaatatata ctataaagag taatactctg
      781 atccagataa aaaacttcct gaacagatac tttttaaatg agggagaaga agattctgag
      841 tatgatagat tgttagccga gatagatgag gggataagaa aggctattga aacactggag
      901 cctgtagaac tttcccctca gaatgcaaga gtaagaagaa ttcagcatca gattatagaa
      961 aaatatcagt tacactctga tagtgtagga gaagaacctt atagaagagt aaggatattg
     1021 ccctgatgtc attttttact ttagaaggta ttgatggtag cggaaagagc actgtaagtt
     1081 ctattataca aaaagagctg aaaaagagaa atgccaattt tatagctgtg agagagcctg
     1141 gcagtacagg tatcggagag aagataaggg agctactgtt agatccagat gcatttatct
     1201 ctccaataga agaggtttat ctctatgcta caagcagagc ggtgtttgtg aggagaatag
     1261 tccttccagc tcttaattct ggtaaggttg taatatcaga gagatacgtt gattcttctt
     1321 tcgcatacca gggatatggc aaaggggtgc cttaagatct atatatgaga taaataaaca
     1381 agctatatca ggagtatccc caaacttaac agtcctttta gatatagacc cctccacagc
     1441 tctaaaaagg ctggagggta aaaagaagga caggctggaa ttagaaggtc tagattttta
     1501 tgagagagtg agggatggtt atttgaagct ggcagaatta ttcaggtata gatatatggt
     1561 tgttgatggg acaaaagagc cgggagaaat agcctcgatt gtactggata aacttgtgag
     1621 gtttatgcaa tgaatctggg aacactgata aggcttgtct ctgtcattgc aggttcaatc
     1681 gtactgtggt atctgtccaa ttcaataggt aattatttaa tattaaagat tggtaatctt
     1741 caatacagag gggataagca gcgcatcatc accctaggct ctcttataac ctggataata
     1801 agagttatta taggtgtaac tgcagtaact tgggtcttga gattattaaa catagacccg
     1861 agccctttta tcgctggggc agggatactg ggtctggctg taagttttgg tgctcagaac
     1921 ctcataaggg atacgattaa cggatttttt ataatactgg aaaatcagta cgatattggc
     1981 gatgaggtaa agataggtgc tattcagggg acagtagaga gtttaacatt aaggattacc
     2041 agggtcaggg acgattcggg agcgttatat attgtcccca acagtacaat ctctcaggta
     2101 gctaatctga gtgacaggtg gtccagaata ctgtatacgt taaatgtaag taataaagaa
     2161 cccatagaaa agcttgaaag agttataagt tatgcaaaag acaaactgca agacaaatat
     2221 gataaagaat taatagaggg tccaaggata gatggtatac aggcgtttga tgctgcgact
     2281 ttaaa
//
LOCUS       DAOTKU010000273         2275 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_38285_length_2275_cov_3.123423, whole genome shotgun sequence.
ACCESSION   DAOTKU010000273 DAOTKU010000000
VERSION     DAOTKU010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38285_length_2275_cov_3.123423"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="P5310_06515"
     CDS             <1..627
                     /locus_tag="P5310_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614530.1"
                     /note="back-translocating Elongation Factor EF4; binds to
                     the ribosome on the universally-conserved alpha-sarcin
                     loop; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HRU33049.1"
                     /translation="QFPYYSEVEKVEEPYVRVSIISPSEFIGPVMELCLGKRGVLIDM
                     NYIDPIKVVFTYELPLSEVMFDFFDKLKSVTRGYASLDYEPIGFRESDLVKLDILVKG
                     EPVDALSIIVHRDESEKRAREIVKKLRALIPRQLFEIPIQASVNGKIIARENIPPIKK
                     NVLQKCYGGDVTRKKKLLEKQKEGKKRLKQIGQVEVPQEAFLAILKSE"
     gene            620..1747
                     /gene="hemW"
                     /locus_tag="P5310_06520"
     CDS             620..1747
                     /gene="hemW"
                     /locus_tag="P5310_06520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HRU33050.1"
                     /translation="MNEIALYVHIPFCIKKCFYCDFNSFRFDISLFHRYRSALMKEIE
                     LYSNKNYIIESIYIGGGTPSIFPAEDIEEIINALSSSGEVKDDIEFTIEVNPGTIDRE
                     KLERYRALGINRVSIGVQTLRREELTTLGRIHTSEEALCSIQLAKDAGFTNINVDLIY
                     GIPGQTLESWKDTLKRVLDLDITHISIYGLMYEPGTPLYKSLIRGEVSPIPEDTEVEM
                     FNITQDILRDYSFFWYELSNYAREDYECRHNITYWEGKDYLGIGSSSHSLIENTRYSN
                     TPNIEAYIEILSKNKTPRVWIEKLTPLERARELIMLGLRMKRGVSLSNIFNRTGVDVK
                     EIFAKNIEKLISLGLVNYDGDRLTLTEYGRLLGNEVFVEFC"
     gene            1800..>2275
                     /locus_tag="P5310_06525"
     CDS             1800..>2275
                     /locus_tag="P5310_06525"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HRU33051.1"
                     /translation="MIDREYALSLLDRYVKSDNLKKHMLATEAIMRALAEKFNQDEDL
                     WGIAGLVHDIDYELCGEDTSQHGVLAVDILKEAGFPEEIIEAVKMHTKGVEVDDNPMY
                     IAICAADAVNGLITAGALIKPEKSLNAIDTKFIMKRFKEKAFAKGANREEIKLCEK"
BASE COUNT          755 a          331 c          544 g          645 t
ORIGIN      
        1 caatttccat attatagtga ggtagaaaag gtggaggaac cctatgtcag ggtatccatt
       61 ataagtccat ctgagtttat aggacctgta atggaactgt gtcttggtaa gagaggggtc
      121 ttgatcgata tgaactatat agaccctatt aaagtggtat ttacgtatga actcccatta
      181 agtgaagtta tgtttgattt ctttgataaa cttaaatcag taacccgtgg atacgcctct
      241 ctggattatg aacccatcgg tttcagggaa tccgaccttg tcaagttgga catactggta
      301 aagggtgagc ctgtagatgc gttgagtatt attgttcata gagatgagag tgagaagaga
      361 gccagggaga tagttaagaa attaagagca cttatacccc gtcagctttt tgaaattcct
      421 attcaggctt cggtgaatgg aaagattatt gccagagaaa atattccacc tataaagaag
      481 aatgtcctgc agaaatgtta cggtggagac gtaaccagaa agaaaaaatt acttgagaaa
      541 cagaaagaag ggaaaaagag gctaaagcag ataggtcagg tggaagttcc tcaggaggct
      601 ttcctggcta ttcttaagag tgaatgagat agctctttat gttcacattc cgttctgtat
      661 aaagaaatgt ttttactgtg attttaattc tttcagattc gatatatctc tttttcatcg
      721 gtacaggtct gctcttatga aagagatcga attgtattct aataaaaatt atattataga
      781 gtcaatctat attggtggag gaacaccaag catctttccc gcagaagata tcgaggagat
      841 aatcaatgct ctatcctctt caggagaagt aaaagatgat attgagttca cgattgaggt
      901 aaatcctggc acgatagaca gagaaaagtt agagaggtac agagctctgg ggataaatag
      961 agtaagtatc ggagtacaaa ctctcaggag agaagagtta actactcttg gtagaattca
     1021 tacttcagaa gaggctttat gctctattca gctggctaaa gatgcgggct ttacaaatat
     1081 aaatgtcgat ttaatctatg gaataccggg gcagacactg gaaagttgga aggatacttt
     1141 gaagagggtc ctggatctgg atattactca tatatcaata tatggattga tgtatgagcc
     1201 tggaactcct ctttataaat ccttgataag gggagaagtc agtcccatcc ctgaggatac
     1261 agaggtggag atgttcaata taacacagga catattaaga gattatagct ttttctggta
     1321 tgagctctca aactatgcca gagaagatta tgaatgcaga cataatataa catactggga
     1381 agggaaagat tatcttggga ttggttcttc atctcattct cttatagaga acacaagata
     1441 ttcgaatacg ccgaatatag aagcttatat agaaatactg agcaagaata aaactcccag
     1501 agtatggata gaaaaactta cccccttgga gagagccaga gagcttatta tgcttggact
     1561 gaggatgaaa agaggtgtat ctctgagtaa tatatttaat cggacagggg tggatgtaaa
     1621 agagatattt gctaaaaaca ttgagaagct tatctctttg ggacttgtta attatgatgg
     1681 ggatcggcta actctgaccg agtatggcag acttctgggt aatgaggtct ttgtggagtt
     1741 ttgctgacta taatgtagta taatactttt tatcataaag attttaagga ggaagataga
     1801 tgatagatag ggaatatgct cttagtttgc tggatagata tgtaaaatcg gataatctaa
     1861 agaaacatat gcttgcaaca gaagcgataa tgagggctct tgctgaaaaa tttaatcagg
     1921 atgaggatct gtgggggatt gctggacttg tccatgacat agactacgaa ttatgtggtg
     1981 aggatacgtc ccaacatggt gtactggcgg tagatattct caaagaggca ggctttcctg
     2041 aagaaatcat agaggcagtt aaaatgcata caaaaggggt ggaggttgat gacaacccca
     2101 tgtatatagc tatatgtgca gcagatgcgg tgaatggact tataaccgcc ggggctttaa
     2161 ttaaacctga gaagagtcta aatgcgatag atacaaagtt tataatgaaa agattcaagg
     2221 aaaaagcttt tgccaaaggg gctaacagag aagagataaa attatgtgag aagtt
//
LOCUS       DAOTKU010000274         2270 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_38385_length_2270_cov_3.782393, whole genome shotgun sequence.
ACCESSION   DAOTKU010000274 DAOTKU010000000
VERSION     DAOTKU010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38385_length_2270_cov_3.782393"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="P5310_06530"
     CDS             <1..220
                     /locus_tag="P5310_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HRU33052.1"
                     /translation="MEIYTTEPAIVLYTGNWLDIKGKGGRFYSRYSGLCLETQHCPDS
                     PNHPNFPSTILRPGESFSSTTVYKFGII"
     gene            231..1244
                     /locus_tag="P5310_06535"
     CDS             231..1244
                     /locus_tag="P5310_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003442724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HRU33053.1"
                     /translation="MKAVYLERPEDIYIKEIDEPLCPAGYARVRVRAVGICGSDISAY
                     KGTSPMCTYPRIIGHEVVGDIVEVNSSSSQLKVGDRVILEPYIYCGKCYPCLNGRTNS
                     CENLKVLGVHIDGGMTEYISHPVHLIHKLPEKLSDEEWVMVEPLSIAMHALHRLKVKE
                     GEHIAIFGGGQIGNLAAQSALIIGAIPILIDLLDERLGKAREVGIENVLNPQKDNIME
                     KIKEITGGRMAECVVEATGALSAIRTTLDVVACTGRIAFVGWPSQEISLPTALITRKE
                     LDIYGSRNSVGEFPGAIDLISTGRVDVKPVISKVVSLEEIPEYVRILAEEPGRFLKVI
                     GKL"
     gene            1256..2098
                     /locus_tag="P5310_06540"
     CDS             1256..2098
                     /locus_tag="P5310_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HRU33054.1"
                     /translation="MKILRKLFILSLLFFLTSNIYPATVVRGRVTVKGSDTMVILVQL
                     LAEEYMTRYRGKIIQVTGGGTGIGVAGLINKVTDIANASRPLTSEERERIKKAWGKEP
                     KEVKIALDGITIYVHPENPIKEISLEQLKSIYTGKVKSWKDFGWEDRPIIVYTRENNS
                     GTYVFFKEHVLDNEDYTNRAQSLPGTGAVVDAVSKEKYSIGYGGVAYSKGVKILPVKT
                     DNKSKAYLPTEANIRKGLYPISRALYMYTCNYDDPDVKSFIDWILTPDGQKIVSKVGY
                     FPLK"
     gene            2103..>2270
                     /locus_tag="P5310_06545"
     CDS             2103..>2270
                     /locus_tag="P5310_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HRU33055.1"
                     /translation="MKKVLFICTHNSCRSQMAEGLLKTLYGGYYESYSAGLEATEVNP
                     YAIRVMKEIGID"
BASE COUNT          721 a          361 c          563 g          625 t
ORIGIN      
        1 gatggagata tacactacag aaccagccat agtactttat actggaaact ggctggatat
       61 taaaggcaag ggtggaagat tttacagcag atattcaggg ctctgcttgg agacacagca
      121 ttgtcctgat tctccaaatc atccaaactt tccatctact atcttaagac caggagaatc
      181 attctcttcc acaaccgtat acaaatttgg cataatctag gagaagtatg atgaaggcgg
      241 tttatttaga gagaccagaa gatatttata taaaggagat agatgagccc ctctgtcctg
      301 caggttatgc cagggtaagg gtaagagcag taggcatctg tggttctgac atctctgcgt
      361 ataaaggtac ctctccaatg tgcacttatc ctaggataat agggcatgag gtggtgggag
      421 acatagtaga agtaaatagc agttcttctc aacttaaggt tggagataga gtgattcttg
      481 aaccgtatat ttactgtggg aaatgttacc cctgtctgaa tggaaggaca aattcctgtg
      541 aaaatctgaa agttcttggc gttcatatag atgggggtat gaccgagtat atatcgcatc
      601 cggtccatct tatccataaa ttgcccgaga aactttctga tgaggaatgg gtaatggttg
      661 aacctctttc tatagcaatg catgctcttc acaggttaaa ggtaaaggag ggagaacaca
      721 tagcaatttt tggcggggga cagataggga accttgctgc acagagcgcc ctgattattg
      781 gtgctatccc gatccttata gatttactgg atgaaagact gggaaaagcc agagaagtcg
      841 gcatagaaaa tgtccttaat cctcagaaag ataacattat ggagaaaata aaagaaatta
      901 ccggtggaag aatggctgaa tgcgttgtgg aggctactgg agccttgtct gcaataagaa
      961 ctactcttga tgtggtagcc tgcacaggta ggattgcctt tgttggctgg cctagccagg
     1021 agataagtct tcccacagcg ttaataacca gaaaggagct tgatatatat ggctctagaa
     1081 atagtgtggg agaattccct ggtgcgatag atcttatatc taccggtaga gtcgatgtga
     1141 aacctgttat aagtaaggtg gtctctcttg aagagatacc tgaatatgta agaatacttg
     1201 cagaagagcc aggtagattt ctgaaagtaa ttggtaagtt atagggggta tgtcgatgaa
     1261 aatattaagg aagcttttca ttttatctct gttatttttt cttacctcaa atatatatcc
     1321 agccactgtt gtaaggggta gagttacagt aaagggttct gatacgatgg ttatacttgt
     1381 tcagttattg gcagaagagt atatgacaag atatagaggt aagattattc aggtaacagg
     1441 aggtggaact ggaataggtg ttgccggact tataaataag gttactgata tagctaatgc
     1501 gtcgagacct ttgacgtcgg aggagagaga gcggataaag aaagcctggg gaaaagaacc
     1561 aaaggaagta aagatcgccc tggatggtat cactatatat gttcatcctg agaatccgat
     1621 taaagagatc tctctggaac agctgaagag catatataca ggtaaagtta aaagctggaa
     1681 agattttgga tgggaggata gaccaataat agtttataca cgcgaaaata attctggtac
     1741 atatgtattt tttaaagaac acgtgttgga taatgaagac tatactaaca gagcgcagag
     1801 tttacccggg acaggggctg tggtggatgc agtttcaaaa gaaaaatatt ctataggtta
     1861 tggtggagtg gcttactcta aaggggtaaa gatattgcct gtaaagaccg acaataaatc
     1921 taaggcgtat ctaccaacag aggctaatat aagaaaagga ttatatccta taagtagagc
     1981 cctgtatatg tatacctgta actatgatga tccagatgtg aaatctttta tagattggat
     2041 tttgactcct gacgggcaaa agattgtatc caaagttggt tacttcccat tgaagtgatc
     2101 tgatgaaaaa ggttctattt atatgtactc acaactcctg caggtcgcag atggcggaag
     2161 gattattgaa gactctctat ggaggttact atgagtctta cagtgctggc ttagaggcaa
     2221 ccgaagtgaa cccctatgcg ataagagtaa tgaaagagat agggatagat
//
LOCUS       DAOTKU010000275         2270 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_38401_length_2270_cov_2.512415, whole genome shotgun sequence.
ACCESSION   DAOTKU010000275 DAOTKU010000000
VERSION     DAOTKU010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38401_length_2270_cov_2.512415"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1656)
                     /locus_tag="P5310_06550"
     CDS             complement(<1..1656)
                     /locus_tag="P5310_06550"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRU33056.1"
                     /translation="MRSYHFIILIILIISSISNASAKDISPILLAGDKKLPPIEYLDA
                     EGNPNGFVVDLVKELSKEIGIPIEIRLTSWNDAISSLEKGEVDGCQFMRITEEREKLF
                     DFIPIIENFSVIVVPEGSTIENFSYLRGEKVACEKGDVSYDFLKEISDVTVKETQEDV
                     LRAVLDKEVSGGVVGYYSARWMIMENNWQDRLLILPDKLFTNYSGIALRKGSEYLPIL
                     EEGMSILKEKGIYNSLLIKWLGEEPEIRERLAREEKVSLIILIIAVLLVVLTIVLFLL
                     RGYLKREVDRQTLRIKLLLKRERDRYGELKTIYDFLHKSASVVDLKQLGDLFLESVRA
                     IIPGREIKIFRKTDNSYQLWIAHPLEESYSIMEDRIDVKYFIRPKGEIFGFLTVTPDI
                     PKENKAAIDILIKEFEYIITREEDREELFRMQNLYSLVEEFSQLESRSEREIILKDVL
                     RRIVFIVRAEAGSIMILNSDDNKLYIKASVGLPYELEKEVVLELGEGIAGWVALNKTP
                     LILEDTIRDPRYKTSPDTPESDIKSSICLPIIHRDKVIGVLNLN"
     gene            complement(1694..2140)
                     /locus_tag="P5310_06555"
     CDS             complement(1694..2140)
                     /locus_tag="P5310_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU33057.1"
                     /translation="MQITTFSGEGAVYRFRQGFEAITGVASHEALNIRVFKRYALNTD
                     REISDTEIKIKFAIDPETKRLVVVGGESRATFKAKNNRYSNLLIEDPDEIKQMIKDLL
                     RDIILLESVHNPDDPFTEERLNSLERKLDGLRLEASIKGILVDVTL"
BASE COUNT          648 a          520 c          332 g          770 t
ORIGIN      
        1 gttcagattc aaaaccccaa ttaccttatc tctatgtatt atcgggagac atatggatga
       61 ctttatatca gactcaggtg tatcaggaga agtcttatat cttggatctc tgatagtatc
      121 ctccaggatt aatggggtct tattaagtgc cacccaacca gctatccctt ctcccagttc
      181 caaaaccacc tccttttcta actcatatgg tagtcctaca gaggctttta tgtatagttt
      241 attatcgtca ctgtttagga tcataattga acctgcctct gctctaacga taaatactat
      301 ccttctcaat acatctttca gtattatctc tctctcagat cgggattcta actgcgaaaa
      361 ttcttcgacc agagagtaca ggttttgcat tctgaataac tcttctctgt cctcctctct
      421 tgttataata tattcaaatt cctttatgag gatatctatc gctgctttat tctccttagg
      481 gatatcaggt gttaccgtca aaaatccaaa gatttcacct tttggtctta taaaatactt
      541 aacatctatt ctgtcttcca ttatactata cgattcctct aggggatggg ctatccagag
      601 ctgataggag ttgtcagtct ttctaaatat ttttatctcc ctaccaggta ttatagccct
      661 tactgattct aagaataaat cccccagctg ttttaagtct accactgatg cacttttgtg
      721 aagaaaatca tagatagttt ttagctcgcc gtatctatca cgttctcttt tgagtaacag
      781 ttttattctg agtgtttgtc tatctacctc tctcttgaga tatcctctaa gtagaaaaag
      841 aacgatagta agtactacta acaggaccgc aataattaaa ataatcaggg atactttttc
      901 ctctctggct aacctttctc ttatctctgg ctcttccccc agccacttga taagtaatga
      961 gttatagatt cctttctcct ttaagataga catcccctct tccaggattg gcaaatattc
     1021 gcttcccttt cttaatgcta taccactata attagtaaat agtttatccg gaagtattag
     1081 aagtctatcc tgccagttat tttccattat catccaccgg gcagagtaat atccaacaac
     1141 tccaccagac acttctttat ctaatactgc cctcaataca tcttcctggg tctctttaac
     1201 tgtaacgtca gaaatttctt taagaaaatc ataagagaca tctcccttct cgcaggcaac
     1261 tttttcccct ctgagataag aaaaattttc tattgtggac ccctcgggaa ctactataac
     1321 actgaaattc tctataatcg ggataaaatc aaataacttt tccctctcct cagttatcct
     1381 cataaactgg caaccatcca cttctccttt ttccagggaa gaaatagcat cattccagct
     1441 ggtaagacgt atctcaattg gtatacctat ttcttttgat aattccttaa ccagatccac
     1501 aacaaagcca ttaggattac cctcagcatc gagatattca atcggtggga gttttttatc
     1561 acctgccagc agtatgggcg aaatatcttt ggctgatgca ttcgagatag aagatattat
     1621 caaaataatc aatattataa aatgatatga tctcataacc ttatgattat atcattaatg
     1681 acttgaaaaa aatttacagg gttacatcta ccagtatacc cttaatagac gcttctaatc
     1741 tcaaaccatc aagctttctt tctaaagagt taagtctttc ctcagtaaac ggatcatccg
     1801 gattatgaac gctctctaaa aggatgatgt cccttagcag gtcctttatc atctgtttta
     1861 tttcatcagg gtcttcaatc aatagattgg aataccgatt atttttagcc ttgaatgtcg
     1921 ctcttgattc tccaccaacc actaccaatc tcttcgtctc agggtcaata gcgaatttta
     1981 tctttatctc tgtatctgat atctctctat ctgtgttcag ggcatatctt ttgaataccc
     2041 tgatattcaa tgcctcatga gaggctaccc ctgtaatcgc ctcaaaaccc tgtctaaaac
     2101 gatatacagc tccctctcca ctgaaagttg taatctgcat ggttaacctc ttcctgaaaa
     2161 atattaacat atagaaaatc aataagcaag agagctactg cagactagtc taatattatg
     2221 accctatatc ttcctccgtt aatgggtttt cccggcgagt gattataaac
//
LOCUS       DAOTKU010000276         2266 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_38488_length_2266_cov_2.670737, whole genome shotgun sequence.
ACCESSION   DAOTKU010000276 DAOTKU010000000
VERSION     DAOTKU010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38488_length_2266_cov_2.670737"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            36..917
                     /gene="flgL"
                     /locus_tag="P5310_06560"
     CDS             36..917
                     /gene="flgL"
                     /locus_tag="P5310_06560"
                     /inference="COORDINATES: protein motif:HMM:TIGR02550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HRU33058.1"
                     /translation="MRITYNMTANTVLRNLTNNLTRIEKLQEQLSSGKKVSQPSDDPL
                     ATIRIMEYKTTLSANDQYTRNTDFLKTELEVADKSLQDISSTLNRVKTLAVRGASESL
                     PQSSRDAIAEELDQLIDHLIQVANTDVSGKYIFGGYRTTIPPLERQGDVINYRGDNNV
                     RMVEISKDVTIETTPTGKTLFVDTGMFEHLISLKNALLGGDTSQINEEMGNLDKIMDR
                     VNAENSSLGARLSRIEFNSNLISDKNVRYTDLLSKQEDIEIEDVVLKLYSQQNIYQAS
                     LIAAARVLQPSLIDHLG"
     gene            924..1355
                     /locus_tag="P5310_06565"
     CDS             924..1355
                     /locus_tag="P5310_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009657596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HRU33059.1"
                     /translation="MKVRTLLFGDIEIEDKYKIEFVSPILGFEDEREFVLIREKDDSP
                     FFWLQSLKTPELAFLLADPFIFFPDYSINIEDSHIGEKIAIYVIVALNEDFKLSTANL
                     IAPIVIDIESKKAFQIVLEDSPYTTRHHLFEDKISNSALVG"
     gene            1357..1575
                     /gene="csrA"
                     /locus_tag="P5310_06570"
     CDS             1357..1575
                     /gene="csrA"
                     /locus_tag="P5310_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002582016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HRU33060.1"
                     /translation="MLVLTRKRGQSILLGDNIEVTILEINRDSVKIGINAPKNVEILR
                     KELYEEIKRTNLESIVKSRDILKELSKE"
     gene            1579..>2266
                     /locus_tag="P5310_06575"
     CDS             1579..>2266
                     /locus_tag="P5310_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003487359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HRU33061.1"
                     /translation="MEIIICQLYPDLMNLYGDRGNIFVLIKRAEWRDIDVKLIPVSIG
                     DDVPEEVDIFFMGGGQDREQRLLAPDLTKEKKVSLISAAERGVVFLGICGGYQLFGHY
                     YKPENTPRLDGLGILDVYTEAGEKRFIGNVLVKSMIDGYRDLTLVGFENHSGRTYLGK
                     NARPLGEVIRGYGNNGSDKTEGAWQNNVFGTYLHGPLLPKNPWLADLLLERALSHKYG
                     EIKLPLLDDSL"
BASE COUNT          720 a          361 c          526 g          659 t
ORIGIN      
        1 taatagagaa aacaggggtc gttgggaggt aagatatgag aataacatat aatatgacag
       61 ccaacacagt actgaggaac ctgacgaata atcttaccag gatagaaaaa ctgcaggagc
      121 agctatccag cgggaaaaag gtaagtcaac cctcagatga tccactggct actataagga
      181 ttatggagta taaaaccaca ttaagtgcta atgatcagta taccagaaat acagattttt
      241 taaagacaga attagaggtg gcagataagt ctttgcagga tatctcttct acccttaaca
      301 gggtcaagac cctggcagtg aggggagcta gcgaatcttt acctcaatct tccagagatg
      361 ctatagcaga ggagttagat caacttattg atcatcttat tcaggttgct aatacagatg
      421 tttcggggaa atatatattt ggaggataca gaacaacgat tcccccatta gagagacagg
      481 gagatgtcat aaattataga ggtgataata acgtcagaat ggtggagatt tctaaagatg
      541 taactattga gactactcca acaggtaaga cattatttgt cgataccggt atgtttgaac
      601 acctgatttc ccttaaaaat gcccttttgg gtggggatac ttctcagata aatgaagaga
      661 tgggaaatct tgataagatt atggatagag tgaacgctga gaattctagc ctaggtgcca
      721 gattatctag aatagaattc aacagtaatc tcatctctga taaaaatgtc agatatactg
      781 acctcctgtc aaagcaggaa gatatagaaa tagaagatgt ggttttaaaa ctttattctc
      841 agcagaatat atatcaggcc agtctcatcg ctgctgccag ggtattacag cctagcctga
      901 ttgatcatct tggttaggtg cttatgaagg tgaggactct tttatttgga gatatagaga
      961 tagaggataa atataagata gagtttgttt ctccgatcct tggttttgag gatgagaggg
     1021 agtttgttct tatcagggaa aaggatgatt ctcctttctt ctggttacag tctttaaaga
     1081 ctcctgagct ggcatttctt ctggctgacc catttatctt cttccccgat tattcaataa
     1141 acatcgaaga ttcacatatc ggtgaaaaga ttgcgattta cgtgatagtt gctttaaacg
     1201 aagattttaa actttctaca gcaaatttaa tcgccccaat agttatagat attgaaagta
     1261 aaaaggcttt ccagatagtt ctggaagatt ctccttatac taccaggcat cacctctttg
     1321 aagataagat ttctaactct gctctggtag gctgagatgc ttgttcttac cagaaagaga
     1381 gggcagtcta tacttctggg ggataatata gaggttacta ttcttgagat aaacagagat
     1441 agtgtaaaga ttggtattaa tgctcccaag aatgtggaga ttctcagaaa agaactctat
     1501 gaagagataa aaaggacaaa tcttgagtcc atcgtaaaga gcagggatat cctgaaggaa
     1561 ttgtctaaag aataatctat ggagatcatt atatgtcaac tctatcctga tcttatgaat
     1621 ctctatgggg ataggggtaa tatttttgtc cttataaaga gagcagaatg gagagatata
     1681 gatgtgaaat taatccctgt ttctattgga gatgatgtgc ctgaggaggt agatatattt
     1741 tttatggggg gaggacagga tagggagcag agattgttag ctcctgatct tactaaggaa
     1801 aaaaaggtta gtttaatctc agcggcagag aggggtgtgg ttttccttgg gatctgtggt
     1861 ggttatcagc tctttggtca ttattataaa cctgagaata ctcccagact ggatggactt
     1921 ggtatactcg atgtctatac agaagctgga gagaagaggt ttataggtaa tgtccttgtt
     1981 aagtctatga tagatgggta tagagatctg actctggttg gatttgaaaa tcacagtggc
     2041 aggacatatc tgggtaagaa tgccagacct ctcggagagg ttataagagg ctatggtaac
     2101 aatggctcag ataaaaccga aggagcctgg cagaataatg tctttggaac atatctccat
     2161 ggacccctgc taccaaaaaa tccgtggtta gcagaccttc ttcttgaaag ggctttatca
     2221 cataagtacg gagagataaa gcttcctctt ttagatgatt ctctgg
//
LOCUS       DAOTKU010000277         2263 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_38564_length_2263_cov_2.865942, whole genome shotgun sequence.
ACCESSION   DAOTKU010000277 DAOTKU010000000
VERSION     DAOTKU010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38564_length_2263_cov_2.865942"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /locus_tag="P5310_06580"
     CDS             complement(<1..701)
                     /locus_tag="P5310_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HRU33062.1"
                     /translation="MRTREELERISMSLEEYELARSYLGREPNDLELGMIAVMWSEHC
                     GYKHSKKTLKLFPTSGENVLQGPGENAGIIRLDEELALAMKMESHNHPSAIEPYQGAA
                     TGVGGIVRDILAMGARPVALLDSLRFGTLDNPRTRYLFSGVVSGISGYGNCIGVPTIG
                     GELAFEKPYTENPLVNVTCLGLLKGKPKSGKAEGTGNSLILVGASTGRDGIHGATFAS
                     LELTSSSIERRPAVQA"
     gene            complement(698..1348)
                     /gene="purQ"
                     /locus_tag="P5310_06585"
     CDS             complement(698..1348)
                     /gene="purQ"
                     /locus_tag="P5310_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HRU33063.1"
                     /translation="MNFGIVVFPGSNCDRDCYYVIKEILNQPARFIWHQETDLNGIDC
                     VILPGGFSYGDYLRAGAIARFSPVMSEVKEFADRGGLIVGICNGFQILTEAGLLPGAL
                     GRNKGLKFICRYVSLRVERNDTPFTTEYKRGDIIRMPIAHNEGNYYIDNPDKIRNQVV
                     LRYYGENPNGAIDDIAGIVNERGNVFGLMPHPERCSEKILGSEDGLKIFKSIVRAL"
     gene            complement(1345..1572)
                     /gene="purS"
                     /locus_tag="P5310_06590"
     CDS             complement(1345..1572)
                     /gene="purS"
                     /locus_tag="P5310_06590"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HRU33064.1"
                     /translation="MLIRLKQGILDAQGSVILNALKSLGFNEVLEVRVGKLLEIKLDR
                     KVDEAGRIERMCKELLANPVMEDYEWEWIEE"
     gene            complement(1603..2263)
                     /locus_tag="P5310_06595"
                     /pseudo
     CDS             complement(1603..2263)
                     /locus_tag="P5310_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062180.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
BASE COUNT          580 a          571 c          331 g          781 t
ORIGIN      
        1 gcctgtacgg caggtcttct ctctatagag ctgctggtaa gttccagtga ggcaaatgtt
       61 gctccatgaa tcccatctct tcctgtagat gcacctacca gtataagact attccctgta
      121 ccttctgcct ttccactttt aggttttccc ttcagtagac ccaaacatgt tacatttacc
      181 agaggatttt ctgtatatgg tttttcaaaa gccagttctc caccaatagt tgggacacct
      241 atacagttgc catatcctga tataccactt accacaccag aaaagaggta tcttgttctg
      301 ggattatcta gagttccaaa tctcaatgaa tctaaaagag ctactggtct tgcccccata
      361 gccagtatgt ctcttacaat accacccacg ccggtagctg caccctgata tggttctata
      421 gcagagggat gattatgact ttccatcttc atagctagtg ccagctcttc atctagtctt
      481 attattccgg cattctctcc aggaccttga agtacatttt ctcctgatgt tgggaagagc
      541 ttcagtgtct tttttgaatg tttatatcca cagtgttctg accacataac cgctatcata
      601 cctaattcta aatcattagg ctctctccct aaataactcc tggcaagttc gtactcttcc
      661 agagacatcg atattctttc cagttcttct ctcgttctca tagcgccctc actatagatt
      721 taaagatttt taagccatct tcagacccga gtatcttttc gctgcatctc tctggatgtg
      781 gcataagtcc aaagacatta cctctttcat ttactatacc ggcaatatca tctatcgctc
      841 catttggatt ttctccataa tacctcagga ctacctgatt tcttatctta tcaggattat
      901 ctatgtagta gtttccctcg ttatgagcta taggcattct tataatatct cctcttttat
      961 attcggttgt gaatggagta tcatttctct ccaccctgag tgatacatat ctacatataa
     1021 attttaatcc cttatttctt cccagagctc caggaagcag accagcctca gtaagtatct
     1081 ggaatccgtt gcatatacca acaatcaatc ctcccctatc ggcaaattct tttacttctg
     1141 acataactgg ggagaatctt gctatagctc ctgccctcag ataatctccg tatgaaaaac
     1201 ctcccgggag tattacacaa tctatcccgt ttaaatccgt ttcctgatgc cagataaatc
     1261 ttgccggctg gtttaatatc tcttttatta catagtaaca atctctatca cagtttgatc
     1321 cgggaaatac aactatacca aagttcattc ttctatccac tcccattcgt aatcttccat
     1381 aaccggatta gccaagagct ctttacacat tctctctatc ctcccagctt catctacttt
     1441 tctatccagt tttatttcca gtagctttcc aaccctgacc tctaacactt cattaaaacc
     1501 gagtgacttc aaggcattta atattacgga gccctgagca tcaagtatcc cctgctttaa
     1561 tcttataagt atccttgttt ttgattctac aggggtctct aagactctct tttttacctc
     1621 gttataagcc tcctctactc ctccaaggtc tcttctgaat ctgtctttat cccatgattc
     1681 ggtagttcct aattcccaca gtctgcaggt atctggagat atctcatcgg caagatatat
     1741 ctctccactt gaatctttgc caaattcgaa cttcatatcc accagatcca gatggcacaa
     1801 tttaaagaac tcaataagta tctggttggt tttgagagac atatctttta tagttattaa
     1861 ttcttcagga gaagctaaac ccaaagctga tatatggtct tcatttatca tcgggtcatg
     1921 gagttcatca ctcttataac agaattccag gattggcttc tccagttttc taccttcctc
     1981 cagtccaaga cgttttgcca tgcttccagc tactttattc cttatgatta cctcaatagg
     2041 aaaaattttt acgcttctta ccagcatttc tctctcatta agtctctcta tatagtgatt
     2101 ccttactcct ttaatggtaa gtaattcaaa tatcttgctg gatattgcgc aattagcctc
     2161 tcctttaccc tgaatctctg cttttttaag accatcaaat gcagtagcag aatctttaaa
     2221 ctctataata aatttatcat cttcttcggt gctatatacc ctc
//
LOCUS       DAOTKU010000278         2263 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_38567_length_2263_cov_2.455163, whole genome shotgun sequence.
ACCESSION   DAOTKU010000278 DAOTKU010000000
VERSION     DAOTKU010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38567_length_2263_cov_2.455163"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(124..1434)
                     /gene="amrA"
                     /locus_tag="P5310_06600"
     CDS             complement(124..1434)
                     /gene="amrA"
                     /locus_tag="P5310_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HRU33065.1"
                     /translation="MSIDYAFVTPHPPLIIPDVGGRYLGDVAATVESMKELGRRAKEI
                     SPETIIVVSPHCELFLRYFGIVTQERIEGDFGEFGAPNVRLQFDIDLDLTQKIIDSAK
                     KSDLPVREIRDVYLDHGAMVPLYYITRELSYKPKLVLLSFSLLPMEEIYSFGKVIDSV
                     AEGKVLFVASGDMSHRLIPGAPAGYDPMGEVFDQAMVEILKNGNLKALLEIDPDLAER
                     AGECGLRSLIMLAGIMDGKRIRTRFLSYEGPFGVGYLVGEVISVDSNDLTSYILNLAK
                     EAVETYVRERKVIVPKNIPEELNRRAGAFVTIKKHGELRGCIGTIQGTQPNLAYEIIQ
                     NAISSATQDPRFPPVREEELPELTYSVDILNPPEKVTDISQLDPRIYGVIVERGWQRG
                     LLLPDLEGVDTVEEQLRIAALKAGLNINEIENIYRFTVTRYGEK"
     gene            complement(1446..>2263)
                     /locus_tag="P5310_06605"
     CDS             complement(1446..>2263)
                     /locus_tag="P5310_06605"
                     /inference="COORDINATES: protein motif:HMM:NF020260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU33066.1"
                     /translation="IIMNLKLPLNFIDVNIHPAKLEIKILEEGRVYSEVYNGIYEAIR
                     GKDISYKTSDREKPVMEIREAPATIEREEISSYEQKILIPEEVKDEEGIFPLEPVGQY
                     MNTFIICTSSNGIYLVDQHVAHERVLFDSFGEIKGIPQFLLEPRYIEVSSANYELIEL
                     IVNNLNQIGFECDISGPGGIVVRAIPSFLKDVDIESAISDYLESKDLDLNNLKREVAC
                     KSAVKLGDRLSMEEMRELLKNLSRIRNYKLCPHGRPIIIQLIDRETVFKKFGR"
BASE COUNT          581 a          572 c          375 g          735 t
ORIGIN      
        1 gtccaacgat gcctatctta tctccggtat aaatatcaag agaaaattta tcaaagagta
       61 ccttttcacc gtatgatttg gcaaggtctc ttattcgtat ggttaacaga aagaatcacc
      121 cccttatttc tctccatatc ttgttactgt gaacctgtat atattctcta tctcgtttat
      181 gttcagtcct gcctttaatg ctgctatcct cagctgttct tctactgtat caaccccttc
      241 taaatcgggt aaaagcagcc ctctctgcca tcccctctcc actatcacac catatatcct
      301 cgggtcaagc tgtgaaatat ctgtaacctt ttctggtggg ttgagtatat ctaccgagta
      361 tgtaagttct ggtagttctt cctctcttac aggggggaat cttgggtcct gtgtggcaga
      421 gcttatagcg ttctgtataa tctcataggc taaattaggc tgagtcccct ggattgttcc
      481 gatgcatcct ctcaattctc catgtttctt tatggtaaca aatgccccgg ctcttctatt
      541 cagttcttcc gggatatttt ttgggactat cacctttctc tctcttacat atgtctcaac
      601 agcctctttt gccagattca gtatgtaaga tgtcagatca tttgaatcga cagagatgac
      661 ctcacctacc agatatccca caccaaacgg accctcataa gagaggaatc ttgtccttat
      721 ccttttccca tccataatgc cggctagcat tatcagtgac ctgagtccgc attctccagc
      781 cctctctgcc agatcggggt ctatctctaa tagggctttc agattcccat tttttaatat
      841 ctctaccatc gcctgatcaa aaacctcacc catagggtca taaccggctg gagctccagg
      901 tatcaatctg tgtgacatat caccactcgc tacaaataag accttcccct ctgccactga
      961 atctataacc ttcccaaatg agtatatctc ttccatcggt agtaaagaga atgagagaag
     1021 caccagtttt ggtttatagc tcaattctct ggttatgtaa tacagtggga ccatagcccc
     1081 atggtccagg tatacatccc gtatctctct tactggaagg tcagactttt ttgccgaatc
     1141 tattatcttc tgagttaagt ccaggtctat atcgaactga agtcttacat ttggtgcacc
     1201 aaactctccg aaatcaccct ctatcctctc ctgagtaact atcccgaagt atcttaaaaa
     1261 taactcacag tgtggtgaga cgacgataat tgtctcgggt gatatctctt ttgcccttct
     1321 ccccagctct ttcattgatt ctacggttgc tgccacatcc ccaagatatc ttccccctac
     1381 atcgggtatg ataagtggag gatgtggagt tacaaacgca taatctatac tcataggtcc
     1441 cctccttatc taccaaactt tttaaagact gtctcacggt ctatcagctg tataataatt
     1501 ggtctaccgt gaggacagag tttgtaattt cttatcctgg ataaattttt caatagctct
     1561 ctcatctcct ccatagagag tctatctccc agttttaccg cactcttaca tgccacctct
     1621 ctcttcagat tattgaggtc caggtctttg ctctccagat aatcgcttat agctgattct
     1681 atatcaacat ccttcaaaaa ggatggtatg gctctcacta ctatcccacc aggacctgaa
     1741 atatcacact caaagcctat ttgattaaga ttattcacaa taagctctat aagttcgtaa
     1801 ttcgcactgg aaacctcaat atatcttggc tccagcagaa attgagggat tccctttatc
     1861 tcaccaaaag aatcaaacag gaccctttca tgtgcaacat gctggtcaac aaggtatata
     1921 ccattacttg aggtgcagat aataaatgta ttcatatact gcccaacagg ctcaaggggg
     1981 aaaattccct cttcatcttt aacctcttca ggaattaata tcttctgttc gtaagaactt
     2041 atctcttctc tctcaatggt agcaggagct tcccttatct ccataacggg cttttctcta
     2101 tcagatgtct tatatgagat atcctttcct cttatcgcct cgtagattcc attgtatact
     2161 tcactgtaga ctctcccctc ttccaggatc ttgatctcaa gtttagcagg atgtatattc
     2221 acatctataa aattcaatgg aagtttcagg ttcattatga tga
//
LOCUS       DAOTKU010000279         2254 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_38764_length_2254_cov_3.466121, whole genome shotgun sequence.
ACCESSION   DAOTKU010000279 DAOTKU010000000
VERSION     DAOTKU010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38764_length_2254_cov_3.466121"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(108..1070)
                     /gene="mltG"
                     /locus_tag="P5310_06610"
     CDS             complement(108..1070)
                     /gene="mltG"
                     /locus_tag="P5310_06610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HRU33067.1"
                     /translation="MAALLKSLIVIICVSILLISFFLYPPLPYKGVEMVTIKPGMNAR
                     DVAALLKEKGLISSEKIFLYLLKLNDKETKLVSGLFDIPRGLRVDALVRFIFETKPKT
                     VWVTIPEGFNSREIAERLEVNGVISREEFLDALNREIKDDYPSFIRPPYEGFLFPDTY
                     EFYIESTPEAVIKKFLDRFVAVLPEDFEEKAKNLGLTPREAIILASLIEKEARIDEER
                     PVIAQVLLKRLNIGMKLQCDATVQYALGKSKPILSYDDLKVKSPYNTYLYYGLPPGPI
                     CNPGLPSIISAVNPADTDYLFYFTRGDGVHIFSRTYEEHLRSQR"
     gene            complement(1076..2159)
                     /gene="prfB"
                     /locus_tag="P5310_06615"
     CDS             complement(join(1076..2114,2113..2159))
                     /gene="prfB"
                     /locus_tag="P5310_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008194827.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HRU33068.1"
                     /translation="MKKEILELEKRYLELGVVIFDKQISKLSELEEETQREDLWSDPR
                     EAQKVLSQISKLKGVVEKGKKVGGLIESLKEFSDLLEEDESIEKELQEELKNANRLLD
                     ELEIQVLFTEEYDNYNAVVTIHSGAGGVDAQDWAEMLLRMYTRWAERNGFNLEIINTS
                     YGDEAGIKSATFIVKGDFAYGYMKAERGVHRLVRISPFDAAHRRHTSFALVDVIPDIG
                     NEIEVEIKVEDLKIDTFRASGPGGQYVNKTESAIRITHIPTGIVVECQSERSQYQNRE
                     TAMRILKAKLFALEKEKREKEVQTLKGERKDIAWGNQIRSYVLHPYTLVKDHRTGVEV
                     GNIQAVLDGDIDVFISAYLKSMMLISK"
BASE COUNT          619 a          544 c          359 g          732 t
ORIGIN      
        1 tttatcttca accaataaaa gttttccatc taaagtttca gctacagatt gttgtaagct
       61 catagagaaa aggaaaataa ataagactga atacagacaa aattttttca cctctgactc
      121 ctcagatgtt cttcgtatgt cctagagaat atatggaccc catcccctct ggtgaaataa
      181 aaaagataat ctgtatcggc aggatttact gcagagatta tagagggaag gccgggatta
      241 catattggac ctggtggaag tccataataa agataagtat tataggggga cttaactttc
      301 aagtcatcat aacttaagat aggcttactc ttccccagtg catactgaac ggttgcatca
      361 cactggagtt tcataccaat atttaatctc ttgagtaaga cctgggctat gaccggtctt
      421 tcctcatcta ttcttgcctc tttctctata agagaggcaa gaattatagc ttctcttggt
      481 gtaagtccta aattcttagc tttttcttca aagtcctccg gtaatacagc tacaaatcta
      541 tccagaaatt tttttataac ggcttccggg gtactctcta tataaaattc atatgtatca
      601 gggaagagaa acccctcata agggggacgt ataaatgatg gatagtcatc ttttatctcc
      661 ctatttaggg catctaaaaa ttcctccctg gatataactc cgttaacttc cagcctctct
      721 gcaatctctc tggagttgaa tccttccggt atcgtcaccc agacggtctt tggttttgtc
      781 tcaaagataa acctgaccag agcatctacc ctgagtcctc tgggtatatc gaatagacca
      841 gagacaagtt ttgtttcttt atcgtttaac ttcagcagat ataagaaaat cttctcagaa
      901 gatatcaagc ccttttcttt taacagtgca gcaacatctc tggcattcat tcctggtttt
      961 atggttacca tttctacccc cttatatggc aagggtggat ataagaaaaa agatatcaaa
     1021 agaatagaaa cacatattat aactatcagg gattttaaaa gggcagccat aatctctatt
     1081 tacttataag catcatactc ttaagatagg cagatataaa gacatctata tctccatcta
     1141 aaactgcctg tatattccca acttctacac ctgtcctgtg gtcctttacc agggtgtatg
     1201 gatgcagtac ataggaacgg atctgatttc cccacgctat atccttcctc tcacccttga
     1261 gcgtctggac ctctttttct ctcttctctt tctcaagggc aaaaagcttc gccttaagta
     1321 ttctcatagc cgtttctcta ttctgatact gagacctctc actctgacat tcaactacta
     1381 tcccagtagg gatatgggta attctgattg ctgattcagt cttattcaca tattgaccac
     1441 caggaccact ggctctgaat gtatctatct tcaaatcttc tacttttatc tccacctcta
     1501 tctcatttcc tatatcaggg atgacatcca ctagggcaaa agaggtatgt ctcctgtgag
     1561 cggcatcaaa gggagatatc cttaccagcc tgtggacccc tctttcagcc ttcatatatc
     1621 cgtatgcaaa atctcccttg actataaatg tagcactttt aatacctgcc tcatctccat
     1681 aggaggtatt tataatctcc aggttgaatc cattcctttc agcccatcgt gtatacatcc
     1741 ttagcagcat ctctgcccaa tcctgagcat ccactccacc tgcaccagaa tgtatggtta
     1801 caactgcatt gtagttatcg tactcctctg taaagaggac ctgaatctcc agttcatcta
     1861 aaagtctatt ggcatttttc agctcttcct gtaattcttt ctctatactc tcatcctcct
     1921 ccagaaggtc agagaattcc ttgagagact ctataagacc tccaactttc ttcccctttt
     1981 ctacaacccc ctttaatttg cttatctgag aaaggacctt ttgagcctct ctggggtctg
     2041 accagagatc ttctctttga gtctcttctt ctaactcaga caacttcgaa atctgcttgt
     2101 caaagataac cacccaactc taagtatctc ttctcaagct ctaatatttc tttcttcacg
     2161 aaacatccct tccacaacag tatttatatt ttttcccact accacagggg caggggtcat
     2221 ttcttcctat cttctttacc cttacagatt tttt
//
LOCUS       DAOTKU010000280         2248 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_38914_length_2248_cov_3.548564, whole genome shotgun sequence.
ACCESSION   DAOTKU010000280 DAOTKU010000000
VERSION     DAOTKU010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38914_length_2248_cov_3.548564"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="P5310_06620"
     CDS             complement(<1..605)
                     /locus_tag="P5310_06620"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/oligopeptide/nickel ABC transporter
                     ATP-binding protein"
                     /protein_id="HRU33069.1"
                     /translation="MEDRAIIRLEHLSKSFTVRSGVFGGRKSMQVIGDLSLSINEGEV
                     LCLVGESGCGKTTTGKLIVGLIKPTSGKILFEGQDIWSMSKEDFSSYRRAVQIVHQDP
                     YSSLNPVHRIYNILSAPLFRHGIVRSRGEAEEKVLELLGRVDLTPPEDFIYKYPHQLS
                     GGQRQRVAIARSLTLNPRFIVADEAVSMLDVSIRLSVIELL"
     gene            complement(595..1587)
                     /locus_tag="P5310_06625"
     CDS             complement(595..1587)
                     /locus_tag="P5310_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRU33070.1"
                     /translation="MAEEILNVENLFVEYSNVRGKVKAVRGVNLKLYKGENLALIGES
                     GSGKTTLGTAIIRLLPSSGYIKEGRIVYKGNENGYDISKFSEKELRQFRWKEIAMVFQ
                     SALNSFNPVLKIWDQIYDTARSHGLNNRSLVREKATDILRYVQLDPERVINSYPHELS
                     GGMRQRVLIAMSLLLEPKILILDEPTTALDILTQRNIIELLRIIKKDMGLSMLFISHD
                     LSLAAELADRVAVMYAGAIVELGGVEDIFYRPRHPYTLGLIKAVPTVTAGFRELYSIP
                     GSPPDLISLLPGCKFAPRCEHKEAGCENEEPLLVEVNSGHLVACFKHKEISNGR"
     gene            complement(1590..>2248)
                     /locus_tag="P5310_06630"
     CDS             complement(1590..>2248)
                     /locus_tag="P5310_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRU33071.1"
                     /translation="DIFSQIVNGGKDVIIVAFLAGVISTLIAVTLGSLSAYAGGVTDM
                     VIMFITEIILTIPQFPLLAVLSAFVRLTNPTMLALIIGLLGWPALARAIRAQVLSIRE
                     RDYIEAARVLDLGRLHIIFSEILPNMLSYIVISLIMAMTSAVYSQVGLIMLGLVPFYG
                     HNWGLMIQLAWTRGAIFYRGSIFYILSPIIAIALFQWSAVTFTRSLEGIFNPRLRSGE
                     "
BASE COUNT          676 a          536 c          365 g          671 t
ORIGIN      
        1 ctcaataatt ctataacact gagtcttata gagacgtcca gcatagatac cgcctcatct
       61 gccactataa atctgggatt tagtgttaaa gacctcgcta tagctaccct ctgtctctgt
      121 cccccactaa gctgatgcgg atatttatat ataaaatctt ctggcggagt aaggtctact
      181 ctacccagga gttccaggac tttctcttct gcttctcctc tactcctcac tataccatgt
      241 ctgaataggg gagcacttaa gatattgtaa attctatgca cagggttcaa agaggagtat
      301 gggtcctgat gtacaatctg aacagccctt ctataactgc taaaatcttc tttagacata
      361 ctccatatat cctgaccttc aaatagaatc ttcccagatg ttggttttat caaacctaca
      421 ataagtttac ctgtagttgt ttttccacag ccactttcac ccacaagaca caggacctct
      481 ccctcattta tagacagaga aaggtctcct ataacctgca tactttttct ccccccaaag
      541 acaccacttc tcactgtaaa tgatttactc aaatgttcaa gccggataat tgctctatct
      601 tccattgctg atctccttat gtttgaagca ggctacaaga tgtcctgaat ttacctctac
      661 cagtagaggc tcttcattct cgcaacctgc ctccttatgc tcacatctgg gggcaaactt
      721 gcatccaggt aaaagactta taaggtctgg cggagaacca ggtatggagt aaagttctct
      781 aaaaccagcg gtcacagtgg gaactgcttt tataagcccc aatgtatatg gatgccttgg
      841 tcggtaaaat atatcttcta caccaccaag ttctacaata gctccagcat acattactgc
      901 caccctatcc gcaagttcag ctgcaaggga aagatcatga gagataaaaa gcatagacaa
      961 tcccatatcc ttttttatta tcctcaagag ttcaattata ttcctctgag tcaatatgtc
     1021 caatgcagta gttggctcat caaggataag tatctttggt tccagaagca ggctcattgc
     1081 tataagaacc ctctgtctca ttcccccgct cagttcatga ggataagaat ttataaccct
     1141 ctcagggtct aattgaacat atctcagaat atcagtagct ttctctctaa caagagatct
     1201 attatttaaa ccatgagacc tggctgtatc atatatctga tcccatatct tcagtactgg
     1261 attaaaagag ttcaatgcag actggaaaac catcgctatc tctttccatc taaactgtct
     1321 taactctttt tcgctgaatt tacttatatc atatccattt tcattacctt tataaacaat
     1381 cctgccttct tttatataac ctgaagatgg taataatctt ataattgctg taccaagagt
     1441 ggttttaccc gaaccgctct ctccaatcag agcgagattt tcacctttat aaagttttag
     1501 atttactccc cttaccgcct ttaccttacc cctcacattc gaatattcta caaatagatt
     1561 ttctacatta aggatttctt cagccaatgt tactctcctg atctcagtct tggattaaat
     1621 attccttcta aagacctggt aaaagtaact gcagaccact ggaacagtgc tatcgctatt
     1681 attggggata agatataaaa gatacttcct ctgtagaata tggcacccct ggtccaggca
     1741 agctggatca taagtcccca gttgtgtcca taaaaaggaa caagaccaag cataatcaat
     1801 cccacctgac tgtatacagc agatgtcatt gccataatga gacttataac tatatagcta
     1861 agcatattcg gtaatatttc agaaaatatt atgtgaagcc ttccaagatc cagaactctt
     1921 gcagcttcta tataatccct ctccctaata gagagaacct gagctcttat agcccttgcc
     1981 agggcaggcc atccaagaag accaataata agagccagca ttgtcgggtt ggtgagtctt
     2041 acaaatgcag acaggacagc caggagagga aattggggaa tggtaaggat tatctcagta
     2101 ataaacataa taaccatatc tgttacacca ccagcataag cactcaaaga acccagtgtt
     2161 actgctatta atgtagatat aactcctgct agaaatgcaa ctattataac atccttaccc
     2221 ccattaacta tctgtgaaaa gatatccc
//
LOCUS       DAOTKU010000281         2247 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_38939_length_2247_cov_3.904653, whole genome shotgun sequence.
ACCESSION   DAOTKU010000281 DAOTKU010000000
VERSION     DAOTKU010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38939_length_2247_cov_3.904653"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="P5310_06635"
     CDS             complement(<1..550)
                     /locus_tag="P5310_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU33072.1"
                     /translation="MSREIALDTISLKETPRIAHTEYSLNYHTDYILKKTEASKIDLD
                     TIRKFYDILMIDFLWNTDDGLHGDWEKFGRATDMGHAEYAIDGSDKRIPKECPFKSPE
                     DVWNFDTVAEYGLPDFDEQVSAYESNIQRLRDDFPNQLSTGGYYKTIVSGAIQAFGWE
                     MFLLALSDISKMEKVIDSFFRFT"
     gene            complement(547..1326)
                     /locus_tag="P5310_06640"
     CDS             complement(547..1326)
                     /locus_tag="P5310_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011274539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRU33073.1"
                     /translation="MMSILDMFKLDGRNAIVTGAAQGLGKAMAQALGEAGANVAIVDI
                     NLDLAKDVAGEFSERLGRKFIAIKADVTVKEEVDRMVEDVISGLGCIDILVNNAGIVR
                     HSPAEDMSKKDWDDVIDVNLTGVFLCSQAAGRRMIEQRRGRIINMASMSGLIVNYPQP
                     QVSYNASKAGVIMLTKSLAVEWARYNINVNAIAPGYMKTAMTAPFFEEEEYRRLWLEP
                     TPMKRPGEPEELCGTVVYLASDASSFMTGQVLVIDGGYTLL"
     gene            complement(1333..>2247)
                     /locus_tag="P5310_06645"
     CDS             complement(1333..>2247)
                     /locus_tag="P5310_06645"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HRU33074.1"
                     /translation="SSARRDLVKEYVDACRKYDMRVGFYFSLLDWRYDAYFLGPKKDP
                     EGWNKLINYMHAQVEELMTNYGKIDILWYDGGWPYTAEDWKSQELNAMVRRLQPDIII
                     NNRSQLPEDFDTPEQYVPGHFPNPNMPERDWESCMTLNDHWGYCKGDNNWKPPQEVIW
                     NLARCASGGGNYLLNVGPKPDGTIPEESIKILEQVGEWIDKNGESIYGTVRTKVGFPH
                     GVTTLKDNKLYIHVFYWNSEFALAPCKINIKKAYFLETGEEINYTVKGERIIFSGLPE
                     KAPDPLDTVIVLEFEGDITALDPFRPFE"
BASE COUNT          613 a          571 c          367 g          696 t
ORIGIN      
        1 aagtgaagcg gaagaaacta tctataacct tctccatctt actgatatcg gagagagcca
       61 gaaggaacat ctcccatcca aatgcctgta tggctccaga aacaattgtt ttatagtaac
      121 ctccagtaga aagctggttt gggaaatcat ccctgagtct ctgtatattt gattcataag
      181 ctgaaacctg ttcatcaaaa tcaggtagcc catactcagc cacagtatca aaattccaca
      241 catcttctgg gctcttgaac ggacattctt tgggaattct tttatcactt ccgtctattg
      301 catattcagc atgtcccata tcggtagccc taccaaactt ctcccaatca ccgtgaagtc
      361 catcatctgt gttccataaa aaatctatca tcaagatatc atagaatttc ctgatagtat
      421 caaggtctat cttggaagct tcagttttct ttagaatgta atctgtatga taatttaggc
      481 tatactcagt atgtgctatc cttggtgtct ctttcagaga aatagtgtcc agtgctatct
      541 ctctgctcat aacaatgtat atcctccatc aattaccaat acctgaccgg tcataaaact
      601 ggaagcatct gaggcaagat aaaccaccgt cccacagagt tcctccggtt caccaggtct
      661 tttcattggg gtaggttcaa gccataacct ccgatactct tcctcctcaa agaatggagc
      721 agtcatagca gtcttcatat agccaggagc aatagcattt acgtttatgt tatatctagc
      781 ccattctacg gcaagagatt ttgtaagcat tataacgcct gccttggatg cattatacga
      841 tacctgaggt tgtggataat ttactatcag tcctgacata gaagccatat tgataatcct
      901 accccttctc tgctctatca tacgtcttcc tgcagcctga gaacagagga atacaccggt
      961 taagtttaca tctattacat cgtcccaatc cttctttgac atatcttcag ctggagaatg
     1021 ccttactatt cctgcattat ttaccaggat atctatgcaa cccaaaccag atatgacatc
     1081 ctccaccata cgatctactt cctcttttac ggtgacatca gctttaatag caataaactt
     1141 tcttccgagc ctctctgaaa actctcctgc cacatccttc gctagatcaa ggttaatatc
     1201 aacaatagca acattagccc ctgcttctcc caaagcctga gccatagcct tacccaatcc
     1261 ctgagcggca ccggttacaa tagcattcct gccatccagt ttaaacatat ccagaatact
     1321 catcatattc tcctattcaa aaggtctaaa agggtccaat gccgttatgt ctccctcaaa
     1381 ctctaaaact attacagtat ccagagggtc aggagctttt tccggtaatc ctgagaatat
     1441 aatacgttca ccctttactg tataattaat ttcttctccg gtctccaaga agtatgcttt
     1501 tttaatatta atcttacagg gggcgagggc aaattcactg ttccagtaaa agacatgtat
     1561 gtataacttg ttatctttaa gggttgtcac cccatgtgga aatcctacct ttgttctcac
     1621 tgtcccgtag atactctcac catttttatc tatccattct ccgacctgtt ccagtatctt
     1681 tatcgattcc tctggtatgg ttccatcagg cttgggtcct acatttaata agtaatttcc
     1741 ccctccagag gcacatctgg ctagattcca gataacttcc tgtggaggtt tccagttatt
     1801 atctccctta caataacccc agtggtcatt aagggtcata caggattccc agtctctctc
     1861 aggcatatta ggatttggaa aatgtccagg gacgtactgt tcaggcgtat caaaatcctc
     1921 tggcaactgg ctcctgttat ttatgattat atctggctgc agtcttctca ccattgcatt
     1981 taattcctga gacttccagt cttctgccgt atacggccat cctccatcat accagaggat
     2041 atcaatcttt ccatagtttg tcatcaactc ttctacctga gcatgcatat agttaatcag
     2101 cttattccag ccctccgggt ccttcttggg tccaagaaaa tatgcatcat atctccagtc
     2161 aaggagcgaa aagtaaaaac caaccctcat atcatatttc ctgcatgcat ccacgtattc
     2221 ctttacaagg tctctcctgg cagaact
//
LOCUS       DAOTKU010000282         2227 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_39366_length_2227_cov_4.083794, whole genome shotgun sequence.
ACCESSION   DAOTKU010000282 DAOTKU010000000
VERSION     DAOTKU010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39366_length_2227_cov_4.083794"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /locus_tag="P5310_06650"
     CDS             complement(<1..499)
                     /locus_tag="P5310_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU33075.1"
                     /translation="MEDISLVSWGLKGKDLQAVSDSYHLPLTPGVGRYLGKLECTESG
                     TLIGDIISPRPLRIWMDNILIVDEPLWWRSFQRQVRCAVIIPVSAGDILLEVEVGERP
                     RHPEYVDKSCPSRNRENVLKALMETIPDELSLDLSFVKGESVPLSLRYFPGQFIRDGI
                     IYQEVV"
     gene            598..1104
                     /locus_tag="P5310_06655"
     CDS             598..1104
                     /locus_tag="P5310_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009060821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HRU33076.1"
                     /translation="MHKVISVEELPINPFTMIGKEWMLITAGNINLFNMMTASWGCLG
                     VLWNRTVAICFIRPHRYTYKFVNENNFFSLSFYPEEYRDALNICGNYSGRDVDKVRLS
                     GLTPVVDEEYKTIFFEESELALICKKIYFQDINPENFLDKSIFDNYPKKDYHRMFVGE
                     IVKALKKE"
     gene            1126..>2227
                     /locus_tag="P5310_06660"
     CDS             1126..>2227
                     /locus_tag="P5310_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU33077.1"
                     /translation="MVKRPFSVYCDWGLHDELGDNIELDEKMTLDVLDSLKYWKEKGV
                     TFDYYLFDAFWFEPEGDYTQFKKTHWTGGFERVRREIETLGMKPGLWFDVNGFTSPKN
                     EKWKESLTRDGRSYCLFDGPYKEGLYNALVHAYKKWGVRMFKLDFANFRAVTIGLEGR
                     FSDDEIYSRNTVALKDVLKTFRKECPEAVILAYNGYELIPGYIGNTSLPVRKGIDPSW
                     LEVFDYMYSGDPRPADTPCISFRRSLDLYQDHMVYRFNYSGLPFYRIDDHGCMIGDTN
                     TIYYLGKKSWRRSWIMTLIRGSKKAHLYGNLHLLDDSDMEFLRVSRDMFFSLYREGLE
                     TSTIGVPLRDSWHGFLLGGASKGLLALVNSSPT"
BASE COUNT          690 a          396 c          482 g          659 t
ORIGIN      
        1 taactacttc ctggtagata atcccatctc gaatgaattg acctggaaag taacggaggg
       61 ataacggaac agactctcct ttgacaaatg ataaatctaa agataattca tcaggaatag
      121 tttccatcag agcctttaat acattctcac gattgcgaga tggacagctt ttatccacat
      181 attcaggatg tcttggtctc tctcccacct caacctccag taagatatca ccagcggaaa
      241 ctggtataat aacggcacat cgaacctgtc tttgaaaact tctccaccag agtggctcat
      301 ctacaatcag gatattatcc atccagattc tcaaaggtcg gggagaaatt atgtccccta
      361 tcaaagtccc tgattctgta cattcaagtt ttcccaggta ccgtccaacc ccgggggtta
      421 atggtagatg atatgaatcg gacacagctt gtaagtcttt tcccttaagt ccccaggaga
      481 ctaaagatat atcttccata gcaaaacacc tccagattga aaaatattat accctaatta
      541 attgtaatat atttatgcta tgttttatac ttaaaagaaa aaaggaggga atttacaatg
      601 cataaagtta tctcagtgga agaactacca attaatcctt ttacaatgat agggaaagaa
      661 tggatgctta ttaccgctgg aaatatcaac ctgttcaata tgatgactgc aagctgggga
      721 tgcctgggtg ttttatggaa tagaacagtt gccatttgtt tcataagacc acatagatat
      781 acgtataagt ttgtcaatga aaataacttt ttttcactct ctttttatcc tgaagagtat
      841 agagatgccc ttaatatatg cggaaactat tccggtagag atgtggataa ggttagactc
      901 agtggactga cacctgtggt ggatgaagaa tataaaacta tctttttcga agagtctgaa
      961 ttggcactta tctgtaagaa gatatatttt caggatataa atcctgaaaa cttcctggat
     1021 aagagtatat tcgataatta cccgaagaaa gattatcaca ggatgtttgt tggtgaaata
     1081 gtaaaagcct taaagaagga ataatctaaa aaaggaggaa tttttatggt aaagagacct
     1141 ttttctgtgt actgtgactg gggactgcac gatgaacttg gagacaatat agagctggat
     1201 gaaaaaatga ctctggatgt tctggatagc cttaaatact ggaaagaaaa gggtgtcaca
     1261 tttgattatt atctttttga tgccttctgg tttgaacctg aaggagatta tacccagttt
     1321 aaaaaaacac actggaccgg cggttttgaa agggtgagga gagagataga gactcttggt
     1381 atgaaaccag ggctgtggtt tgatgtaaat ggttttactt ctcctaagaa cgagaaatgg
     1441 aaggagagcc tgaccaggga tggaaggagt tattgtttat ttgatggacc ttacaaagaa
     1501 ggtctctata atgccctggt tcatgcatat aaaaaatggg gagtaaggat gtttaaatta
     1561 gattttgcca attttagagc tgttaccata gggcttgaag gaagattttc cgatgatgag
     1621 atctactcaa gaaatactgt cgcacttaaa gatgtcctca agactttcag gaaagaatgc
     1681 cccgaagctg ttatattagc ctataatgga tatgagctga tacctggata tatagggaat
     1741 acctcacttc cagtaagaaa agggatagac ccttcctggc tggaggtctt tgattatatg
     1801 tattcgggtg atccgagacc tgcggatacc ccctgtatct ctttcagaag gtccctagat
     1861 ttatatcagg accatatggt gtacaggttt aactattccg ggcttccctt ttacaggata
     1921 gatgaccatg gctgtatgat aggtgatacc aatactattt actatcttgg caagaaatcg
     1981 tggagaaggt cgtggataat gacccttata agaggaagca aaaaggctca cctgtatgga
     2041 aatttgcatc ttttagacga ttcagatatg gaatttctca gggtttccag ggatatgttc
     2101 ttttctcttt atagagaagg attagagact tccactatcg gtgtgcctct gagagactcc
     2161 tggcatggtt tcctccttgg tggagcctcg aaaggtttgc tggcacttgt aaactcctct
     2221 ccgaccc
//
LOCUS       DAOTKU010000283         2221 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_39521_length_2221_cov_2.223453, whole genome shotgun sequence.
ACCESSION   DAOTKU010000283 DAOTKU010000000
VERSION     DAOTKU010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39521_length_2221_cov_2.223453"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..728)
                     /locus_tag="P5310_06665"
     CDS             complement(<1..728)
                     /locus_tag="P5310_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HRU33078.1"
                     /translation="MKELVGIDVGTRSIKGLVGISDKKLKITGFSVREHPTRAMFDGQ
                     IHNVPQVADSIKFVKEALEKKMKIKITEAATAVAGRVLLTKRGRSQIEFPILTFITES
                     YLNSLEWNAVYSARNLLQSENEMESWDYICVGYSVTRYFLDGVQVESLLGQRGRFISC
                     EIIATFLPRIVIDALVSAFDYAGLKVALMTLEPIAAMDLVVSPDLRRLNIALVDIGAG
                     TSDIAISKDGTVLGYGMVPFAGDEV"
     gene            complement(725..1576)
                     /locus_tag="P5310_06670"
     CDS             complement(725..1576)
                     /locus_tag="P5310_06670"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HRU33079.1"
                     /translation="MSEKLTLDRIIDRIEKFPALPYVISLSLKKIDDPRSSISDITRA
                     ISGDEGLVANVLKLANSAYYGFPRRIVSVTEAVVILGLNTLKSLIYTTLAKDVLGKEV
                     KGYSLDRGELWRHSIACGIICREIARKSRLGDIEAYFVAGLLHDIGKIVLGEYLEEQY
                     EEIVKRVSESLQPFNIIESEVLGFDHAEMGGLLAKRWNLPEFLIEAIRYHHSPEKSKL
                     LSPSVVNVGDGIALMMGYGVGIDGLMYQISEEALARFGLAKEEALEEFISYVDRSLTA
                     AYQFFEL"
     gene            complement(1573..1851)
                     /locus_tag="P5310_06675"
     CDS             complement(1573..1851)
                     /locus_tag="P5310_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU33080.1"
                     /translation="MMYIDDVRMILNYNLDEREKLRVVTDEFVSIFWNALMKEMRKSI
                     DLFSPETPSFAMSTYYEWFDGEISEVLAKSNNSLADILYKELIKNIGG"
     gene            complement(1848..>2221)
                     /locus_tag="P5310_06680"
     CDS             complement(1848..>2221)
                     /locus_tag="P5310_06680"
                     /inference="COORDINATES: protein motif:HMM:NF014206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HRU33081.1"
                     /translation="ISRAIDSSTVEVTVPEEYQSDVVNFLAQIEVLEIIPDIPARIVI
                     NERTGVVVIGKEVRLLPVAIEYNNIKITVVAPQEENAREVTLGDLVQTLNTLGVTPKD
                     TVGIITALKQSGALQGELVFE"
BASE COUNT          652 a          550 c          344 g          675 t
ORIGIN      
        1 acctcatccc cggcaaatgg aaccatccca taacctaaaa ctgttccatc tttagagatc
       61 gctatatcag atgtcccggc acctatatcc accagagcta tatttaacct cctaagatca
      121 ggtgaaacaa ccaaatccat agctgctatt ggctctaggg tcataagcgc tacctttagt
      181 cctgcataat caaaggctga aaccagagca tcaataacta tcctgggaag aaaggttgca
      241 attatctcac aggatataaa ccttcccctc tgtccgagga gagattcaac ctgtacacca
      301 tctaaaaaat acctggtaac actgtatcct acacagatat aatcccagga ttccatctca
      361 ttctctgatt ggagtaaatt cctggcagag tataccgcat tccattcgag agaatttaaa
      421 taagattctg taataaaagt gaggataggg aattcaatct gggaccttcc ccttttagta
      481 aggagaaccc taccagcaac agcagtggcg gcttcagtaa tttttatctt catcttcttt
      541 tccagagcct cttttacaaa tttgatagaa tcagccacct gaggtacatt atggatctgc
      601 ccatcaaaca tagccctggt agggtgctct ctaacggaaa agccggtaat cttcagcttt
      661 ttatcagaaa tcccaactaa cccctttata ctgcgtgttc caacatcaat tccgaccagt
      721 tctttcataa ctcaaaaaat tgatacgcag ctgtaaggga cctatctaca taagagatga
      781 attcttccag cgcttcctct ttagccagac caaacctggc aagcgcttcc tctgagatct
      841 gatacataag tccatctata ccaacaccgt atcccatcat cagggcaata ccatctccaa
      901 cattaacaac actgggggac agaagcttgc tcttctctgg actgtgatga tatcttatcg
      961 cttctatcaa aaactcagga agattccatc tcttagctaa aaggccaccc atctctgcat
     1021 ggtcaaaacc gaggacttca ctttcaatta tattgaacgg ctgaagagat tcggaaactc
     1081 ttttaacaat ctcttcgtac tgttcttcca gatattctcc cagtactatc tttcctatat
     1141 catgtagtaa tcctgctaca aagtatgcct cgatatcccc taacctactc ttccgtgcaa
     1201 tctctctaca tattatgcca caggctattg aatgcctcca taattctcct ctgtctaaag
     1261 agtatccttt aacttctttt cccaagacgt ctttagcaag agtggtatat atcaaactct
     1321 tcaatgtatt gagtcccagg atcacaacag cttcagttac agatactatt ctcctgggga
     1381 agccataata tgcggagtta gccaatttaa ggacattggc aaccaatccc tcgtcacctg
     1441 aaatagctct ggttatgtct gatatagaac ttctggggtc atctattttt ttaagagaca
     1501 aagaaataac gtatggcagg gctggaaact tctctatcct atctataatt ctatccaaag
     1561 ttaatttttc actcatccac ctatgttttt aatcaactcc ttatataata tatcggctaa
     1621 actgttattg ctcttagcca aaacttctga gatctcaccg tcaaaccatt cataatatgt
     1681 agacatagcg aatgacggtg tttcaggaga gaacagatct atagactttc tcatctcttt
     1741 cataagagca ttccagaata ttgatacaaa ttcatctgta actactctca gtttctccct
     1801 ttcatctaag ttataattta atatcattct cacatcatct atatacatca ctcaaacacc
     1861 agctctccct gtaaggctcc ggattgtttc agtgctgtaa ttattccaac tgtatctttt
     1921 ggtgttacgc ctaaagtgtt aagagtctgc actaaatctc caagtgtaac ctctctggca
     1981 ttctcctcct gtggagcaac tacagttatc tttatattat tatactctat tgcaacagga
     2041 agtaaacgga cctctttccc tataaccaca acaccggtcc tttcatttat cactattctg
     2101 gctggaatat caggtattat ctctaatact tctatctgtg cgagaaaatt gaccacatca
     2161 ctctgatact cttccggtac agttacctct actgtagaag aatctatcgc cctggagata
     2221 t
//
LOCUS       DAOTKU010000284         2214 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_39689_length_2214_cov_2.265864, whole genome shotgun sequence.
ACCESSION   DAOTKU010000284 DAOTKU010000000
VERSION     DAOTKU010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39689_length_2214_cov_2.265864"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..807)
                     /locus_tag="P5310_06685"
     CDS             complement(<1..807)
                     /locus_tag="P5310_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HRU33082.1"
                     /translation="MHAHNGISRNNLLVVTVLNFSITAAEMIGGMISGSLSLISDALH
                     NLSDGVAILISYLALKISQRDNNERLTFGYKRVEILSALFNSVVLLAISISLFREAYI
                     KIKDPQPIDGLLMIIVALIGLLANFISVLILRGDSQRSLNIRGAYVHLLSDTLSSVGV
                     VIGGILIYFYKIYFIDPILTFLIGAYIIKECLEIVGETVEILMQATPRSIDINRIKEE
                     IESLEEVKNVHHVHIWRLDDNDIHFEGHINLKENLTVDDTIPVYRKIEDIL"
     gene            981..1364
                     /gene="speD"
                     /locus_tag="P5310_06690"
     CDS             981..1364
                     /gene="speD"
                     /locus_tag="P5310_06690"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HRU33083.1"
                     /translation="MYGPHLMLDCYGSPKSILADVSFLYEVLNELPDIIGMHKIMPPY
                     VFRYDSKEIPEDWGVSGVVLIAESHIAIHTYPEKEYFTVDIFSCKEFDLEKAKEYIID
                     KFKVKRYEFHHIDRGLEFPRSMAEV"
     gene            1364..>2214
                     /gene="speB"
                     /locus_tag="P5310_06695"
     CDS             1364..>2214
                     /gene="speB"
                     /locus_tag="P5310_06695"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HRU33084.1"
                     /translation="MFYTHYVTFGDFPEEYKNIEKASAVIVPVPLEVTGTYLSGTKMA
                     PDEIILASRALEWFDESLEFEPFRKGIATISAPQWERGNLTSCILKLEDLMDELIEEE
                     KFPVVIGGEHILTLGNIRGVLKHYNDISCLILDAHADLRDDYDKTNLSHACVTRRILE
                     LGLKSVVEVGVRSLSREEHEFINSSNSIKVIKARDYLRDEKFFIDSILNLLSDNVFIS
                     IDGDVFDPSTLPMVGTPEPGGLLWYNVINLLEEIFTHKNIVGCDLVEVLGTDRASCFI
                     SARLVYKL"
BASE COUNT          735 a          413 c          425 g          641 t
ORIGIN      
        1 caggatatct tctattttcc tgtaaaccgg tatagtatcg tctaccgtca gattttcctt
       61 aagatttata tgcccctcaa aatgaatatc attatcatca agtctccata tatgtacatg
      121 atgcacgttc ttaacctctt ccagagattc tatctcctct tttatcctgt tgatatctat
      181 acttcttgga gtcgcctgca tcaaaatctc tactgtctca ccaactattt ctagacattc
      241 cttaataata tatgccccta ttaaaaaggt caaaataggg tcaatgaaat aaatcttata
      301 aaaatatatg agtataccac caataactac acccactgat gacaatgtgt cggaaagaag
      361 gtggacatag gcacccctga tattcaggct tctctgagaa tctcctctca gtatcaatac
      421 agaaataaag tttgccagaa gtcctatcaa tgcaacaatt atcatcaaaa gaccatctat
      481 aggttgaggg tctttaatct tgatatacgc ctctctgaat agagagatgg agattgccag
      541 aagcacaact gaattaaata gggcagaaag aatctcaacc ctcttatagc caaaggtaag
      601 tctctcatta ttatctcttt gagagatctt cagggcaaga taacttataa ggatagccac
      661 cccatcgctc aggttatgca aagcatcaga tatcagagac agacttccag aaatcatccc
      721 cccaatcatt tctgcagctg tgatagagaa gtttaaaact gttacgacta aaagattatt
      781 tctgctaatt ccattatgag catgcattat cggtcacctc taatcacaaa catctcagtt
      841 atcctaatta tactataaat attcatccaa ttgaataata accctctctc aaacccatac
      901 aacccattga catttcaatc aatttatccc ataataatgg agctaaatta ataggaaaga
      961 gggagtgtga ataagaagaa gtgtacggtc cacatctgat gctagactgt tatggaagtc
     1021 caaaaagtat actggcggat gtaagcttct tgtatgaggt cttaaacgaa ctgccagata
     1081 ttattggaat gcataaaatt atgcctccat atgtattcag gtatgatagt aaagagatac
     1141 ctgaagattg gggggtctct ggggtagtac tgatagccga gagtcatatc gctattcata
     1201 cttatccaga gaaagaatac tttactgtag atatcttctc ctgtaaagaa tttgatctgg
     1261 agaaggctaa ggagtatatt atagacaaat ttaaggtcaa gagatatgaa ttccaccata
     1321 tagatagagg attggaattc ccaaggagta tggctgaagt gtgatgttct atacacatta
     1381 cgtcactttt ggagacttcc ctgaagaata taagaacata gagaaagcca gcgctgtaat
     1441 agtacctgtt cctttagagg taacaggtac ttatctctcc gggacaaaga tggcacctga
     1501 tgaaataata cttgcatcaa gagctcttga gtggtttgat gaaagcttag agtttgaacc
     1561 tttcagaaaa ggtatcgcaa ccatatctgc ccctcagtgg gaaaggggga acttaacttc
     1621 ctgtatatta aaattagaag acctgatgga tgaattaata gaggaagaaa aattcccggt
     1681 agtaattgga ggggagcata tcttaacgct gggaaacatc agaggtgtct taaaacatta
     1741 taacgacatc tcctgcctta tactggatgc acacgccgac ctgagagatg attatgataa
     1801 aacaaatctc agccatgcct gtgtaacaag gagaatatta gaattaggac ttaaaagtgt
     1861 agtagaagta ggagttagaa gtctctccag agaagaacac gaattcatca attcaagtaa
     1921 ctctataaag gtaattaagg ctagggatta cctgagagat gaaaaattct ttatagatag
     1981 tatacttaat ctcctcagtg ataatgtctt catctctata gatggggatg tctttgatcc
     2041 atccactctc cctatggtgg ggactccaga accgggagga cttttatggt ataatgtaat
     2101 caatctctta gaggagatat ttacacataa gaatatagtt ggatgtgacc tggtagaggt
     2161 tttgggaaca gacagggcat catgctttat atctgccagg ttggtatata agct
//
LOCUS       DAOTKU010000285         2208 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_39815_length_2208_cov_3.614956, whole genome shotgun sequence.
ACCESSION   DAOTKU010000285 DAOTKU010000000
VERSION     DAOTKU010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39815_length_2208_cov_3.614956"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="P5310_06700"
     CDS             <1..340
                     /locus_tag="P5310_06700"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008826625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HRU33085.1"
                     /translation="YDIAHGAGLAIVFPAWMKYVYKHNIERFIQFAINVWNVEPNFES
                     PERIALEGVERLKRFFKDIGLPTSLSEANIGEDRLEEMATKATERGELGEFVKLKKED
                     VLNILRSVLK"
     gene            465..1751
                     /gene="apgM"
                     /locus_tag="P5310_06705"
     CDS             465..1751
                     /gene="apgM"
                     /locus_tag="P5310_06705"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HRU33086.1"
                     /translation="MYKLLYIVLDGLGDRPCRDLNGKTPLEAAETPNMDKLASLGKSG
                     IVDTVGPGIAPESDIAVISILGYDAEKYYTGRGPLECYAIGIDVKPGDLAFRVNFATM
                     DENRYLSDRRAGRDLSTEEAKALAEEVNNKVKLTSHPAEFKFVSTVGHRGVLWIRCKE
                     GPLSANITNTDPAYDKIGYFGVAKEKFEPYLQVCKPLDDSIEARRAADLTNEFVEKSH
                     IVLNNAEVNLKRKAQGKMMGNVILTRDAGDRLPQFPDIKKLFGLNFACFAEMPVEMGI
                     AMLTGMDIVELPEPTGDPEVDYPLRVEKTVEYMKRYDALYIHLKGPDIPGHDGKAIEK
                     RDSISAIDKYFFGGLLPRINLSDVVMAITADHSTPCDLRAHSDDPVPLLIVGAGIESD
                     GTLNYSERECSKGSLGRLLGNQLMPLFVKLIGKGED"
     gene            1762..>2208
                     /locus_tag="P5310_06710"
     CDS             1762..>2208
                     /locus_tag="P5310_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011232729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HRU33087.1"
                     /translation="MGISRAYIEIGRNDPCPCGSGKKYKKCCLPLLEESLSIDSLKFS
                     VYYKIAYHTFTNYKEFFKDTAGKFERKDLASAESFEDLVRNKKEWEEPLDWFIFNEYV
                     LKGKTPLQLFLEEGDATEKEKNILRRFDGTYWSLYEVKDLVKELGKA"
BASE COUNT          691 a          357 c          574 g          586 t
ORIGIN      
        1 atatgatata gcccacggag ctggactggc tatagtattt ccggcatgga tgaagtatgt
       61 gtataaacat aacattgaaa gattcataca gtttgctata aatgtatgga acgttgagcc
      121 gaactttgaa tctccagaga ggattgccct ggaaggggta gaaagactta agagattctt
      181 caaagatata gggcttccca caagcttatc tgaggctaat ataggggaag atagattgga
      241 ggaaatggct accaaggcta cagagagggg tgaactggga gagtttgtaa agttgaaaaa
      301 agaggatgtg cttaatatac tcaggtctgt cctgaagtag attacgacca aaatactggg
      361 gaaggacaaa atccttcccc aaattatatc gttacagtga tgtgatctaa gttatatgtt
      421 ataattgaat agactgaaaa agctactgag aaagaggtga ttgaatgtat aaattattat
      481 acatagtact tgatggatta ggagacagac catgcagaga cctgaatgga aaaactccgt
      541 tagaagctgc cgaaactcca aatatggata aactggccag tcttggtaag agtggtattg
      601 tagatactgt gggaccaggg atagctccgg agtctgatat agcggttata agcatcctgg
      661 gttatgatgc agaaaaatat tatacaggac gtggtccgct ggagtgctat gctattggca
      721 ttgatgtcaa gcccggtgac cttgctttca gagtaaactt tgcaactatg gatgaaaata
      781 gatatctctc agacaggaga gcagggagag atctgagcac agaagaggct aaggctctgg
      841 ctgaagaggt gaataacaag gtgaagctga caagtcatcc ggcagaattc aagtttgtaa
      901 gtacagtcgg gcatagaggg gtcctctgga taagatgtaa ggagggtcca ctatctgcca
      961 atattacgaa taccgaccct gcctatgaca agataggtta ttttggtgtt gccaaggaaa
     1021 agtttgagcc atatctacag gtatgcaagc cactggatga tagtatagag gctaggaggg
     1081 ctgcagatct gactaacgag tttgtagaga aaagtcatat agtcttgaat aatgcagagg
     1141 tgaatcttaa gagaaaagct cagggtaaga tgatggggaa tgttatactt accagagatg
     1201 cgggtgatag acttccacag tttcctgata taaaaaagct cttcgggtta aactttgcct
     1261 gttttgccga gatgcctgta gaaatgggta tagctatgct tacaggaatg gatattgtgg
     1321 agttacctga acctaccggt gatcctgaag ttgattatcc tctgagagtg gagaagactg
     1381 tagaatatat gaagcggtac gatgccctct atatacacct caaaggtcct gatatcccag
     1441 gacatgatgg aaaggctatc gagaagaggg atagtatctc tgcaatagat aagtatttct
     1501 ttggaggtct attacctagg ataaacttat ctgatgtggt gatggcgatt accgctgatc
     1561 attccacccc ctgtgatctc agggctcatt cagatgaccc tgtccctcta ttaattgttg
     1621 gagcagggat agaaagcgat gggactttaa attattcaga gagggaatgc tccaaaggtt
     1681 ctcttggcag gcttttaggt aatcagttaa tgcccctttt tgttaagttg atagggaaag
     1741 gagaagatta atcgaagaaa tatgggtata agtcgagctt acatcgaaat agggagaaat
     1801 gacccctgtc cctgtggtag tggtaaaaag tataaaaagt gctgtctccc actcctggag
     1861 gagtctctgt caatagattc attgaagttc tctgtctatt acaagatagc ctatcataca
     1921 tttaccaatt ataaggagtt cttcaaagat accgctggca agttcgaaag gaaagacctt
     1981 gcttcagctg agagttttga ggacctggta agaaataaga aggagtggga agagccactg
     2041 gactggttta tcttcaatga atatgtcctg aaggggaaga cccctttgca gttatttctt
     2101 gaagaagggg atgctacaga gaaagagaag aatatcctca gaaggtttga tggaacatac
     2161 tggtctctct atgaagtgaa ggatctggtc aaggaattgg gtaaggct
//
LOCUS       DAOTKU010000286         2200 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_39986_length_2200_cov_3.783683, whole genome shotgun sequence.
ACCESSION   DAOTKU010000286 DAOTKU010000000
VERSION     DAOTKU010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39986_length_2200_cov_3.783683"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1060)
                     /locus_tag="P5310_06715"
     CDS             complement(<1..1060)
                     /locus_tag="P5310_06715"
                     /inference="COORDINATES: protein motif:HMM:NF026221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU33088.1"
                     /translation="MAQWWDRPLRMMRWDYMDDFSKIRYEDLESLARMKKEKLHINCE
                     WIVGTPGVSPGLGYLTTFQAEGFERYPGFEDFDSLRSYLPYAHKYGIKLLVYLNMHWF
                     SYDFARKHPGWEQMTSGGESYGRLHPLYGNGTTFCVNSPWRDWAFKLIGEVMKTGVDG
                     VFLDGPVVYPDCCYCDSCREKFRSIYGEDIPEEDWQDPLWKEFVEFREMSMADFLRDA
                     QNAVLKINPNGVIFLNAGSWHPGGWRVARDAKRLARYQNFSLAEAFFHPSSGRHNLYA
                     SAMTTKYLRATGKPAVTAFHYCMGPWHYTFLTPEEIKSSVFQATGCNGNIWVGGATYR
                     YDFKPEGCSPLNETLGFLE"
     gene            1213..2010
                     /locus_tag="P5310_06720"
     CDS             1213..2010
                     /locus_tag="P5310_06720"
                     /inference="COORDINATES: protein motif:HMM:NF016816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HRU33089.1"
                     /translation="MRFYIITDLEGVEGVTLPIQTEAGNPMYEKARRLLTREVNIAVE
                     TLREIGAEEIIVLDGHGANQAYNLVYEELVNGARYVMGSPWSNYLPLLTSNFDGLIQI
                     GAHAMAGTKGAVLDHTMSSRSWRCAYLNDIPVGEMGIAAFYAGTSGVPLIFVSGDDKA
                     CLEAKGLVSDVETAITKYGLTRTSAIMLPPGEVERNIKDGLKRAAEKARFIKPLSLDS
                     PVSFKIEFLLAEHAQRYWNREDVELLDPRTVIFKGKDAREAVERWYL"
     gene            2007..>2200
                     /locus_tag="P5310_06725"
     CDS             2007..>2200
                     /locus_tag="P5310_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU33090.1"
                     /translation="MRILDVNTAFGFYPYRKLDTSLETLKGILGNYNARAFSLSLKGV
                     FYDAREGNEETLNLIGRDNI"
BASE COUNT          646 a          467 c          472 g          615 t
ORIGIN      
        1 cttccaaaaa acccagagtc tcattgagag gggaacatcc ttccggttta aaatcatatc
       61 tgtatgtagc gcctcccacc catatgtttc cattacatcc tgtcgcctga aagaccgatg
      121 actttatctc ttctggagtc agaaatgtat agtgccacgg tcccatacag tagtggaatg
      181 cggttactgc cggtttaccc gttgctctca gatattttgt tgtcatagca gaggcataca
      241 ggttatgcct tccagaagaa gggtggaaga atgcctcagc cagagaaaaa ttctgatatc
      301 tggcaagtct ctttgcatct ctagctactc tccatcctcc tggatgccag gacccggcat
      361 ttaaaaagat aaccccgttc ggatttatct tcaggacggc attctgtgca tctcgtaaaa
      421 aatcagccat agacatctcc ctgaactcta caaactcttt ccatagaggg tcctgccagt
      481 cttcttctgg tatatcctct ccataaatac ttctaaactt ttctctgcag gagtcacagt
      541 aacaacagtc gggatatact accggaccat ccaggaagac cccatctact cctgtcttca
      601 taacctctcc tataagctta aaagcccagt ctctccaggg agaattaaca cagaaagttg
      661 ttccatttcc gtagagagga tgaagcctac catatgactc tccacctgag gtcatctgct
      721 cccagccagg atgcttcctg gcgaagtcat aactgaacca gtgcatattc agatagacta
      781 atagttttat cccgtattta tgggcatatg ggagataact tctcagagaa tcaaagtctt
      841 caaagccagg atatctttca aatccttcag cctgaaacgt cgtcagatag cctaaaccgg
      901 gtgaaacacc aggggttccc acgatccatt cacagtttat atgtaatttc tctttcttca
      961 tcctcgccag actttccagg tcttcatatc ttattttaga aaaatcatcc atatagtccc
     1021 atcgcatcat tcttaatggt ctatcccacc attgagccat aagatacctc catttttaaa
     1081 tttacagtta taatagcacc gttccagtag agtgtcaata ttctgacaat taactggaat
     1141 taagaggagc ttgacttttt attaagtaaa ggtataattc ttatataaat ttaataatta
     1201 cctggaggat ctatgagatt ctatatcatt accgatttag aaggtgtcga gggggttaca
     1261 cttcctattc agactgaggc tggtaaccct atgtacgaga aagcgagaag attgcttacc
     1321 agagaggtaa atatagcggt agagacattg agggagattg gagcagaaga gataatagtc
     1381 ctggatggtc atggagctaa tcaggcttat aatcttgtgt atgaagagct tgtaaatgga
     1441 gccagatatg ttatggggtc tccatggtca aattatctgc cgttgctcac ctcgaatttt
     1501 gatggtttaa ttcaaattgg agctcacgct atggcaggaa caaagggggc tgttcttgac
     1561 catacgatgt cctccagaag ctggcgatgt gcatatctga atgatatccc tgtgggagag
     1621 atgggaatcg ccgcctttta tgccggtaca tcaggagtac cattgatatt tgtctctgga
     1681 gacgataagg cttgtcttga ggcaaaagga ctagtaagtg atgtggaaac tgccataacc
     1741 aagtatggac ttacaagaac ctctgccatt atgctaccac cgggtgaggt ggagaggaat
     1801 attaaagatg gcttaaaaag agctgccgag aaggcaagat ttataaagcc actcagtctg
     1861 gatagcccgg tatcttttaa gatagaattc ttacttgcag aacacgccca gagatactgg
     1921 aatagagaag atgttgaact cttagaccca aggacggtaa tattcaaagg gaaggatgca
     1981 agagaagccg ttgaaaggtg gtatctatga gaatactaga tgtaaataca gcctttggct
     2041 tttatcctta caggaaatta gatacatctt tagaaaccct gaaagggata ctggggaatt
     2101 ataacgccag agctttttcc ctatccttaa agggagtctt ctatgatgcc agggagggga
     2161 acgaagagac tttaaatctt atcggcaggg ataatatata
//
LOCUS       DAOTKU010000287         2186 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_40330_length_2186_cov_2.769122, whole genome shotgun sequence.
ACCESSION   DAOTKU010000287 DAOTKU010000000
VERSION     DAOTKU010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40330_length_2186_cov_2.769122"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1369
                     /locus_tag="P5310_06730"
     CDS             <1..1369
                     /locus_tag="P5310_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRU33091.1"
                     /translation="SGDPALGEWDIYTGGWVTVIVARDTSDNFQYFYLPDSLMSFSPL
                     WKAYNPTKEFRDIGNKLAQRSFKSMKERDLLMTKALHLAMKDSVRIFLVDQNSAWVRR
                     KGVDAIADYAGGYSARIWPYTLRFENKTGGRMRVGSSEVIIDPWNPVAGSNWIYDNMI
                     MEATLGRSYIWHPYDGLPILLNIKGAELTVKKDVPTFKNPDTKWFKFSKVDKVTVPAD
                     AWYAWDIKKQDVVTAGAAGIKEANAKVVVDYGDIIGKDKYHDGSVMSPADFIVDWIIG
                     FERASKDSKLYDESYVDTFNSWRDQFVAWKIKTTNPLVIEYYINYSEIDAELTATAFA
                     SSAYYPWHLYAIGLRAEEEGKLAFSASKAEEKNIEWMNYIGGPSLPILKDVVERCEKE
                     GYVPFKNFVGKYVDTSVAKERFSSMKKFYEKYGHFWVSNGPYYLEKVDVVAHTALLRN
                     SKFLK"
     gene            1589..>2186
                     /locus_tag="P5310_06735"
     CDS             1589..>2186
                     /locus_tag="P5310_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRU33092.1"
                     /translation="MTNIVMGYKDDPSFRNLSEERQKELIESRFKSEMKRRGLDKPFP
                     IRSVYHLKNAITLDLGRAMYMTSDGGSALVKNIILERLPVTVILFTTATVINFFLHLF
                     LGLRLSRKYGSFWDKLVIALSPTSVIPGWFYGIFLIMIFYSWLHILPPGGLVDYPPPS
                     GTIPYFLSILKHMVLPLASWIIASLFLGIYQQRTFFLIY"
BASE COUNT          690 a          396 c          501 g          599 t
ORIGIN      
        1 gagcggagac cctgccttag gagaatggga catatatacc ggtggctggg ttactgttat
       61 tgttgcaaga gatacctctg ataatttcca gtacttctat ctacctgatt cccttatgtc
      121 gttctcacca ttgtggaagg cttataatcc gacaaaggaa tttagagata taggaaataa
      181 attggcacag agaagtttca agagtatgaa ggagagagac ctccttatga ccaaggctct
      241 tcacctggcc atgaaagact ctgtaaggat attcctggta gaccagaact ctgcctgggt
      301 tagaaggaag ggagtagatg ctatagcaga ttatgccggt ggatattcag ccagaatatg
      361 gccatataca ttgagatttg aaaataaaac tggcggaagg atgagggttg gaagctcaga
      421 agtaatcata gacccctgga acccggttgc cggctctaac tggatatatg acaatatgat
      481 aatggaagca acactgggaa gatcttacat atggcatcca tacgatggat tacccatcct
      541 acttaatata aaaggagcag aactcacagt taagaaggat gtccctacat ttaagaatcc
      601 agataccaaa tggttcaagt tcagcaaggt agataaagtt actgtgccag ctgatgcctg
      661 gtatgcctgg gatataaaga agcaggatgt ggtgacagca ggtgcagcag gaataaaaga
      721 ggcaaatgct aaagtagtgg tggactatgg agatatcata ggtaaggaca aatatcatga
      781 cggttcagtg atgagtcctg ccgactttat cgtagactgg ataattggat ttgagagggc
      841 gagcaaagat agtaagcttt acgatgaatc ctatgtcgat acattcaact cctggagaga
      901 ccagtttgtt gcctggaaga taaaaaccac aaatccgctg gtaattgaat actatataaa
      961 ctactctgag attgacgctg aacttaccgc cacagctttc gcttcttccg cttactatcc
     1021 ctggcatcta tatgccatag gattgagggc agaggaagag ggtaaactgg cattcagtgc
     1081 ctccaaggca gaggaaaaga atatagagtg gatgaattac ataggtggac caagcctccc
     1141 catccttaaa gatgtggtgg aaagatgcga aaaagaggga tatgtaccat ttaaaaactt
     1201 tgtagggaaa tatgttgata catctgtggc aaaagagaga ttctcttcta tgaagaagtt
     1261 ctatgagaaa tatggacatt tctgggtaag taacggtcca tactacctgg agaaggtaga
     1321 tgtggtagct cataccgcat tgctcagaaa ctccaagttc ctcaagtaaa tagctgtgcg
     1381 atatataacc cagggaattg tttccctggg ttatctctta tataatagaa taaaaaagga
     1441 aggaggtcga ttataaataa aagctatatt taaatatgta ttaaagagag ttatctctct
     1501 atttctgacc gtagtagttg cagtatatct taccatcgtt atagccaatg ctggtggata
     1561 tgttgacaga atagttgaaa ctgagctgat gactaatata gttatgggat ataaagatga
     1621 cccgagcttc aggaatctat cagaggaacg tcagaaagaa cttatagaga gcagatttaa
     1681 aagtgaaatg aaaagaagag gtctggataa acccttccct atcagaagcg tttatcacct
     1741 taagaatgct ataacattag atcttggcag ggctatgtat atgacaagtg atggtgggtc
     1801 tgccctggta aaaaatataa tacttgagag acttcctgtt accgttatac tctttacgac
     1861 tgcaacggtg ataaattttt tcctccatct gttcctggga ttaagactgt caagaaaata
     1921 cgggtcattc tgggataagc tggtaattgc actttctcca acatctgtaa taccaggatg
     1981 gttctacggt atcttcctca ttatgatctt ttactcctgg ttgcacatac tgccaccagg
     2041 agggttggtt gattatcccc caccttctgg aactatccca tactttttaa gcattctgaa
     2101 acatatggtt ttaccgctgg cctcatggat aatagcatca ttattccttg gaatatacca
     2161 gcagaggaca ttcttcctca tatact
//
LOCUS       DAOTKU010000288         2179 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_40505_length_2179_cov_2.405367, whole genome shotgun sequence.
ACCESSION   DAOTKU010000288 DAOTKU010000000
VERSION     DAOTKU010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40505_length_2179_cov_2.405367"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1528)
                     /locus_tag="P5310_06740"
     CDS             complement(<1..1528)
                     /locus_tag="P5310_06740"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRU33093.1"
                     /translation="MKRFWVIGLVLILVFSLMLGNVPVSFSQKKYNEAPMLAELVKQG
                     KLPPVEQRLPEVPYVVKTMDGAKNARYGGMLRFVRTDPYYDSDGGISNLAQLVHIPGE
                     GGTFKQSGSKMVPDIVQSYEVSEDSKVFTFHLREGLKWSDGYPVTTEDVQFAYEDVLL
                     NDKLTPVFPKDLTAGDNPCKVEIVDKYTFKVRFSRSYGGFLGVLGRRMMGYSWFLLPK
                     HYLKEFHPKYTSIEKLEPLIKEAGFEKGQWWNLFNRKTDPFRGDIGCPVLTPWVMVES
                     NPKTGVIRWERNPYYYKCDEAGNQLPYIDYRESSLLKDPKMVPMKVVAGEVDFMRQFG
                     ELKDLPLYKEYEEKAGIRVLLFQKDVETGYVCLNQSHKDPVWRKIVGDVRFRRALSMA
                     INRKEIINTVYLGLAVPSTLVPSTYDPVKASQLLDQMGLNKRDSEGWRLGPDGKRFEI
                     PFEVPQLMGFEAPMTEMVIDFWKKVGIYATMKTVSFELWDQLGASNEIRASIHWGQAP
                     A"
     gene            complement(1615..>2179)
                     /locus_tag="P5310_06745"
     CDS             complement(1615..>2179)
                     /locus_tag="P5310_06745"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRU33094.1"
                     /translation="RVEIDNKKAAYSATEFLIKLGHRDIACITGPLNTRIAQQRLEGY
                     KRVLIENNIPIRDEFIVEGTFKLDSGFKGVNTLLERDIHFTAIFACNDLMAVGAIKAL
                     RKRNLDVPNDVSVIGFDGIPLCEYIEPALTTVVQPIYEMGKTAAELLLESIREGIELK
                     GHIVLDTRIEVRESTREVRVEGKYKKF"
BASE COUNT          652 a          511 c          288 g          728 t
ORIGIN      
        1 tagcaggagc ttgtccccaa tgaatactag ccctaatttc attcgaagct cctaactgat
       61 cccacaattc gaaacttacc gttttcatag ttgcatagat acccactttc ttccagaagt
      121 ctattaccat ttcagtcatt ggagcttcaa aacccataag ttgaggaact tcaaatggga
      181 tctcaaatct tttaccgtcc ggacctaaac gccaaccttc tgaatctctc ttgtttaatc
      241 ccatctgatc aagaagttga cttgctttaa ctggatcata tgtgcttggt actagggtag
      301 aaggaacggc gagcccaaga taaacagtat taattatctc ttttctgttt atcgccatac
      361 ttaatgctct tctaaagcgg acatctccta caatcttacg ccatacagga tccttatgac
      421 tctgattaag acatacatat cctgtctcta catctttttg aaagagtaga actcttatcc
      481 cagccttttc ttcatactct ttatataatg gaagatcctt aagttctcca aactgtctca
      541 taaagtctac ttcgccagct actactttca taggaaccat tttaggatct ttaaggagag
      601 aagactcgcg ataatctata taaggaagtt gattaccagc ctcatcacat ttataataat
      661 atggatttct ttcccatcta attactcctg tcttaggatt agattctacc atcacccaag
      721 gagtaagtac aggacatcct atgtctcccc taaaaggatc agtctttcta ttaaaaagat
      781 tccaccactg tcctttttca aaacctgctt ctttaataag aggttctagt ttttcgatag
      841 atgtatactt tggatgaaac tctttaaggt agtgttttgg taataaaaac cagctataac
      901 ccatcatacg tcgccctaat actcctaaaa atcctccata acttctagaa aatcttacct
      961 taaaggtata tttatctact atctctactt tacatgggtt atcaccagct gtaagatctt
     1021 ttggaaaaac tggggtaagc ttatcattta ataatacatc ttcatatgca aactgaacat
     1081 cttcagtggt aacaggatat ccatcagacc actttagccc ctctcttaag tgaaaggtaa
     1141 agactttact atcttcacta acttcataac tctgtactat atctggaacc attttactac
     1201 cagattgttt aaaagttcct ccttcaccag ggatatgaac taattgagca agattactga
     1261 tacccccatc agaatcataa taaggatctg tacgcacaaa ccttaacata ccgccatatc
     1321 ttgcattttt agctccatcc atcgtcttta ctacataggg aacttctggt aatctctgct
     1381 ctactggtgg aagtttcccc tgtttcacta actcagcaag cattggagct tcgttatact
     1441 tcttctgaga gaaagagact ggtacatttc ctaacattaa gctaaatacc aaaattaata
     1501 ccaaaccaat cacccaaaat ctcttcattt cttttaacct cctaatcatt ttagtgtttt
     1561 actactaaaa cctaacctca ttgattctaa cccccataaa gaaatttaac ctaattaaaa
     1621 tttcttatat tttccctcca ctctcacctc cctcgttgat tctcttacct caatcctagt
     1681 atccagtact atgtgtcctt ttaattctat cccctctcta atactttcca aaagtaattc
     1741 ggctgcagtt ttacccatct catatatagg ctgaaccaca gtggtaagtg caggctctat
     1801 atattcacat agcgggattc catcaaaacc tattacagag acatcattcg ggacatccag
     1861 gttccttttt ctcaaagcct taatagctcc cactgccata agatcattac aggcaaatat
     1921 ggcagtaaaa tgtatatctc tttctaaaag agtatttact cccttaaatc ccgaatctag
     1981 tttaaaagtc ccttcaacta taaattcatc tctgataggg atattatttt ctattagaac
     2041 tcttttatat ccctctaatc tttgctgagc tatacgagta tttaaaggac ctgttataca
     2101 ggctatatct ctatgtccta actttattaa gaactctgta gcactatatg cagctttttt
     2161 attatctatc tcaaccctt
//
LOCUS       DAOTKU010000289         2176 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_40592_length_2176_cov_2.406412, whole genome shotgun sequence.
ACCESSION   DAOTKU010000289 DAOTKU010000000
VERSION     DAOTKU010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40592_length_2176_cov_2.406412"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..697)
                     /locus_tag="P5310_06750"
     CDS             complement(<1..697)
                     /locus_tag="P5310_06750"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRU33095.1"
                     /translation="MRRVNIQEVAKEAGVSPSTVSRALNGFPGISEKTRERIVEIARK
                     LNYRPNYRGQILTTQNTKNIGLLITDITNPFFPELVMGAEEYASKSGYTVLLGNTSES
                     EEKETNYLDFFSRGPVDGIIISASRVSNEHIIMLAEEGLPIVVINRILEHPKISYVST
                     DMEKGGYLATMHLLRLGHSKIAFINGPKHSEVSQRRLEGYKKALKEAGIDYNSDLISF
                     NVPVSESGYKEAIK"
     gene            829..1845
                     /locus_tag="P5310_06755"
     CDS             829..1845
                     /locus_tag="P5310_06755"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRU33096.1"
                     /translation="MDSREIVIKSLEFEGPERIPMTLPLPYPDDIVGAGLGPNPYVNE
                     EWEYKGNNRWERVDEWGNTWARLEAYSKGEVVKGAIEDWSQYDSYKFPPLDMDSRYEP
                     ARRIFKETPDKFHIGHLPGFPFSIARYLRRMDNFLLDVAAEKEKVARLLKDIADLLEV
                     VMEKFAVAGADAVMFAEDWGTQDRLLVSPRSWRELFKPGFIQLCGKAHKLGLYVFMHS
                     CGHIYEIIPDLIEVGIDVLQFDQPALHGVDNLARDFGGKVNFWCPVDIQQILPKKDER
                     LIEEFAREMIEKLGAFNGGFIAGYYGSNEALGLDPYWQDVACRAFVKYGSMYKNLTKG
                     GLER"
     gene            1887..>2176
                     /locus_tag="P5310_06760"
     CDS             1887..>2176
                     /locus_tag="P5310_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012896257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRU33097.1"
                     /translation="MALSSSSLAFSQEKVIAGEYYNLSDYEKLAGKKIGKYSEAPMLA
                     DLVKQGKLPPVEQRIPKNPLVVTPVEEVGQYGGTWRRAWLGLSDQWGPNKICV"
BASE COUNT          581 a          399 c          540 g          656 t
ORIGIN      
        1 actttatagc ctctttatac cctgattcgg ataccggtac attgaaagat atcaagtcag
       61 aattataatc tatccctgcc tctttcaatg cttttttgta tccttccagc cttctctgtg
      121 agacttctga gtgttttggt ccatttataa atgcaatctt tgaatgaccg agtctcaata
      181 gatgcatggt tgccaggtat ccgccctttt ccatatctgt ggatacataa gagattttag
      241 ggtgttccag tattctattt attacaacta taggaagtcc ctcttctgca agcattatta
      301 tgtgctcatt cgataccctt gaggcagaga ttatgatacc atccaccggt ccccgagaga
      361 agaaatcaag ataatttgtc tctttttctt cagattcaga ggtgtttccc agaagtacag
      421 tataacccga cttacttgca tactcttctg ctcccataac cagttctgga aagaatgggt
      481 tggtgatatc agttataagg agacctatat tttttgtgtt ctgggtagtg agtatctgtc
      541 ccctgtagtt tggtctatag tttagctttc tggctatctc cactattctt tccctggttt
      601 tctcacttat ccctggaaat ccgttcagtg ctctgcttac agtggaaggg gataccccag
      661 cttcttttgc tacctcctga atatttactc tcctcataaa cacctcaata gcgattgcaa
      721 ttttattgca ttttataatg aaaacctctc tctgtcaagg gtattgacaa atagactgct
      781 gttgagatat tataatagtg caatcgcttt tttgagagtg ggagggttat ggatagtaga
      841 gagatagtga taaagagttt agaatttgaa ggaccggaga gaattcctat gaccttacct
      901 cttccttacc ctgatgatat agttggggca gggctgggac caaatccgta tgtgaatgag
      961 gagtgggagt ataaaggtaa taatagatgg gagagagtgg acgaatgggg aaatacctgg
     1021 gcaaggcttg aagcatactc taagggtgag gttgtgaagg gggctataga ggactggagt
     1081 cagtacgatt catacaagtt tcctccactg gatatggact ccaggtatga gcctgccaga
     1141 agaatcttta aagagacccc tgataagttc catattggac atctacccgg atttccattc
     1201 agcattgcga gatatctcag aaggatggat aactttcttc tggatgtggc agcagagaaa
     1261 gagaaagtgg caagattgct aaaagatata gctgatctgc ttgaggttgt tatggaaaag
     1321 ttcgcagtag ccggagcaga cgctgttatg tttgctgaag actggggaac acaggacagg
     1381 cttctggtat cgcccaggtc atggagagag ctgttcaaac cgggatttat acagttatgc
     1441 ggtaaggctc acaaacttgg actctatgtc tttatgcact cctgtggaca tatatatgag
     1501 ataatcccgg atctgataga ggtggggata gatgttctgc agtttgacca gcctgctctt
     1561 catggggtgg ataatcttgc cagagatttt gggggtaagg ttaacttctg gtgtccggtg
     1621 gatatccagc agattcttcc aaagaaagat gagagattga tagaggagtt tgccagagag
     1681 atgatagaga aactgggggc ttttaacggt ggttttatag ctggttatta tgggtctaat
     1741 gaggcattgg gtcttgaccc atactggcag gatgttgcct gcagagcatt tgttaagtat
     1801 ggttccatgt ataaaaattt aacaaaagga ggtcttgaga gatgagtctt ttaaagaagt
     1861 gtgcggtttt attaattact atcgtgatgg ctttaagcag ttccagtctt gccttctccc
     1921 aggagaaggt cattgctgga gagtattaca acctctcaga ttatgagaag cttgctggta
     1981 aaaagatcgg caaatacagt gaagctccga tgttagcaga tcttgtgaag cagggtaaac
     2041 ttccacctgt agagcagaga atacctaaga atcctctagt ggtaacaccg gtggaagagg
     2101 ttgggcagta tggtgggacc tggcgtagag cctggcttgg actctctgac cagtggggac
     2161 caaacaagat ctgtgt
//
LOCUS       DAOTKU010000290         2175 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_40610_length_2175_cov_3.321698, whole genome shotgun sequence.
ACCESSION   DAOTKU010000290 DAOTKU010000000
VERSION     DAOTKU010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40610_length_2175_cov_3.321698"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..171
                     /locus_tag="P5310_06765"
     CDS             <1..171
                     /locus_tag="P5310_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003401479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HRU33098.1"
                     /translation="IEKAKENSESPTMIIAHTIKGKGVSFMENVVDFHGKAPTAQEWE
                     KAKVELGGDYNE"
     gene            164..1093
                     /locus_tag="P5310_06770"
     CDS             164..1093
                     /locus_tag="P5310_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013536907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HRU33099.1"
                     /translation="MSKLAMRDVYGEVLIELGKIYPNLVVVDADVSKSTKTIGFAKTF
                     PDRFFDVGIAEANLMGISAGIASTGKIVFTSTFSVFASGRAYDQVRQSIAYPGRNVKI
                     VSTHCGLTAGEDGAMHQSFEDMALMRSIPNMRVIAPCDGVEVKSVLKNIAEIPGPFYV
                     RLARPATPVIFEESYEFKLGRGTVIKEGKEGVIIACGLMTAIALEASDLLSKEGINIG
                     VVSMSSIKPVDTELIKNLSEQYKFIFSCEDHSIIGGLGGAIAEVLPPTNAKLVRLGME
                     DEFGDTGKFEELLRFYRLDAESIAEKIKSAVKK"
     gene            1090..1767
                     /gene="ftsE"
                     /locus_tag="P5310_06775"
     CDS             1090..1767
                     /gene="ftsE"
                     /locus_tag="P5310_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004227515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HRU33100.1"
                     /translation="MIEFRNVTKYYGNGVLALEDVSFKVEKGEFVFIVGPTGMGKTTL
                     LKLIYREELPTKGKVIVGGVDITRLRSYQVALLRRRLGIVFQDFKLLPNRTVYENVAF
                     AMEVLGYSKKEIRNRVGRVLDIVGLLHKSNCYPNELSGGEQQRTSIARALVNNPPILI
                     ADEPTGNLDMATSWEIMEFLKRINSRGTTIVMATHNKEIVNAMRQRVIGLYNGRIVRD
                     EYNGSYE"
     gene            1764..>2175
                     /locus_tag="P5310_06780"
     CDS             1764..>2175
                     /locus_tag="P5310_06780"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HRU33101.1"
                     /translation="MRVLSIGYILREAWRGLTRARISSFAAIGIIAFMLVLLGIVGYI
                     FNLVNCTFAGVSNQLEIIVYLNDNLKAEERKSLKDNITSIEGVKEVVYIPKNEAWKRF
                     KETFKGNKLVLEIIGENPLPDSFEIKLIDRNYMNY"
BASE COUNT          750 a          338 c          528 g          559 t
ORIGIN      
        1 atagaaaaag caaaagaaaa cagtgagtct ccaactatga taatagccca tacaataaag
       61 ggaaagggcg tttcatttat ggagaatgtg gtggacttcc atggaaaggc tcctacagct
      121 caggaatggg aaaaggcgaa ggttgagctg ggtggtgatt ataatgagta agctggcaat
      181 gagagatgtg tacggagagg tattaataga attaggtaaa atctacccaa acctggtagt
      241 tgtagatgca gatgtatcga aatctacaaa gaccattgga tttgctaaga cttttccgga
      301 tagatttttc gacgttggta tcgccgaagc aaaccttatg ggaatatcgg caggtattgc
      361 ttctacgggc aagatagttt ttaccagtac attttcagta tttgcatctg gtagagctta
      421 cgaccaggtc aggcaatcca tagcgtatcc tggcagaaat gttaagatag tatcaacaca
      481 ctgtggactt acagctggag aagacggtgc aatgcatcaa tcatttgaag atatggctct
      541 tatgcggagt atacccaata tgagagttat agctccatgc gacggtgtag aggttaaatc
      601 ggttttaaaa aatattgcag agatacctgg tccattctat gtaagacttg caagaccggc
      661 tacccctgtg atatttgaag aatcctatga attcaaactc ggtaggggga cagtgattaa
      721 agagggaaaa gaaggagtaa ttatagcctg tggtcttatg acagccatag ctcttgaagc
      781 atcagacctc ctatcaaaag aggggataaa tataggagta gtaagtatgt ccagcataaa
      841 acccgtagat acagagctga taaaaaattt atctgagcag tataaattca tattctcctg
      901 tgaagatcac agtataattg gtggactggg aggtgcaata gcagaggtac tgccacctac
      961 taatgccaaa ctggtacgcc tgggtatgga ggatgaattt ggagataccg gcaaatttga
     1021 agagctgcta agattctaca ggcttgatgc tgaatctata gcagagaaaa taaaatcagc
     1081 agttaagaaa tgattgagtt cagaaatgta accaaatact atgggaatgg agttttagct
     1141 ctcgaagatg tgtctttcaa agtagagaaa ggagagtttg tctttatagt tggacccaca
     1201 ggtatgggca aaactaccct gctgaaactc atatatagag aggagcttcc aacaaaggga
     1261 aaggtaatag tgggtggagt agatattacc agattaaggt cttatcaagt agcgcttctc
     1321 agaagaagac tcgggattgt ctttcaagat tttaagcttc tccccaacag aacggtctac
     1381 gaaaatgttg cattcgcaat ggaagttctc gggtattcca aaaaagagat aagaaataga
     1441 gtggggcgag tattagacat agtagggcta ctgcataaaa gtaactgcta tccaaatgag
     1501 ctctcaggtg gagagcagca gaggacatcc atagccaggg cgctggtaaa taatcccccc
     1561 atacttatag ccgatgaacc gacaggtaac ctggatatgg ctacttcctg ggagattatg
     1621 gaatttttaa aaaggataaa ctcaagagga actactatag taatggctac ccataataaa
     1681 gagatagtaa atgctatgag gcagagagtt ataggtctct acaatggaag gatagtaaga
     1741 gatgaatata atgggagtta tgaatgaggg tattatcaat aggatatatt cttagagagg
     1801 catggagagg actgacaaga gctagaatat cctcttttgc tgctataggg ataatagcat
     1861 ttatgctggt tctattagga atagtaggct atatcttcaa cctggtaaac tgcacattcg
     1921 ctggagtaag taaccagctg gaaataatag tttacctcaa tgataacctg aaggcggaag
     1981 aaagaaaaag tttaaaagac aacataacca gcatagaagg ggtaaaagag gtagtttata
     2041 tccctaaaaa cgaggcatgg aaaaggttca aagagacatt taagggtaat aaacttgtat
     2101 tagagataat tggagaaaat cctcttccag actcatttga gataaaattg atagatagaa
     2161 attatatgaa ttatg
//
LOCUS       DAOTKU010000291         2170 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_40740_length_2170_cov_3.518676, whole genome shotgun sequence.
ACCESSION   DAOTKU010000291 DAOTKU010000000
VERSION     DAOTKU010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40740_length_2170_cov_3.518676"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1113
                     /locus_tag="P5310_06785"
     CDS             <1..1113
                     /locus_tag="P5310_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRU33102.1"
                     /translation="INAVWLGAPREMEMSEKLGVHIPWFILIDPTTNCNLRCVGCWAG
                     EYQQHYSLGKDLMDRIITEAKDLGIHFITVSGGEPFLSKEFIEIAEKHDDVVFHTYTN
                     GTLIDEKMAKKIVELGNIAPAISIEGFEKETDERRGKGVFARATRAMDLLRENGAIFG
                     FSVTATSRNIDVITKDEFIDFLIDKGASFGWMFMYVPIGRDPDLSLMLTPEERAKLRE
                     TVHHWRADKPIFVADFWNDGPYTGGCIAGGRNYIHINGKGDVEPCAFVHFAVDNIKDK
                     SLVEVLKSPLFAEYQKSIPFSDNLLRPCPIVDNPWALREIVTKVGAKSTDGGSEALLS
                     TELANALDKYAESWKEVSDPIWEENYAKKKEEAKVK"
     gene            1115..1444
                     /locus_tag="P5310_06790"
     CDS             1115..1444
                     /locus_tag="P5310_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRU33103.1"
                     /translation="MERAKESEKTFRYGEWGPKYIMRGPRIEWGLLRLIPGKELGAHY
                     HREVEETFYVISGKAQMTVDGETFEISAGDAIRVEAPEEHNIIPSEDFLAIFIKAPYL
                     PEDKYTK"
     gene            complement(1540..>2170)
                     /locus_tag="P5310_06795"
     CDS             complement(1540..>2170)
                     /locus_tag="P5310_06795"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRU33104.1"
                     /translation="YLEKDKPIDDRERRIYAIFAKRLEDVFDTVEILRRRDTAEKNLR
                     RLSLTDELTGIHNRRGFMMLARQHLRLSKRLKKRSILMYMDVDNMKWINDNLGHSTGD
                     EALIEVASILRKTFRKSDVLARIGGDEFVFLGLETEKNNPGILVERIRRKLDARNSKK
                     TSRYPLSLSIGLAVYDPDSPVSLRDLIEEADKRMYQEKMAKKNLAQING"
BASE COUNT          654 a          405 c          481 g          630 t
ORIGIN      
        1 ataaacgctg tatggttggg tgcacctaga gaaatggaga tgagcgaaaa gcttggtgtt
       61 catatcccct ggtttatact tatagacccg acaacaaatt gtaatcttag atgcgtagga
      121 tgctgggctg gagagtacca gcaacactat agccttggta aagaccttat ggatagaata
      181 attactgagg caaaagattt aggcattcac tttattactg tctctggagg agaaccattc
      241 ctcagcaaag aatttattga aatagcagag aaacacgatg atgtggtatt ccatacctat
      301 acgaacggta ctcttataga tgagaagatg gcgaagaaga ttgtggagct cgggaatatt
      361 gctcctgcta taagtattga ggggtttgaa aaggagacag atgagagacg tggtaaggga
      421 gtcttcgcca gggcaacccg tgccatggat ttattgagag agaatggagc tatttttgga
      481 ttttcagtaa ccgccaccag caggaatatt gatgttataa ctaaagatga atttatagat
      541 tttctcatcg ataaaggggc ttcttttgga tggatgttta tgtatgtgcc tatcggaaga
      601 gaccctgacc tgagtcttat gcttacacct gaggaaaggg caaaattaag ggagacagtg
      661 caccactgga gagcagacaa gcctatattt gtagctgact tctggaatga cggtccgtat
      721 actggcggtt gcatagctgg aggaagaaac tacattcata ttaatggaaa gggtgatgta
      781 gaaccctgtg cctttgtcca ttttgccgta gacaatataa aagataagag tctggttgaa
      841 gttctgaaat ctcctctgtt tgcagaatat caaaaaagta ttccgttcag tgataattta
      901 cttagaccct gtccaatagt ggataatccc tgggctttaa gggaaatagt tacaaaggtc
      961 ggagccaaat caacagatgg tggttctgaa gctcttctat ccacagagtt ggctaacgcc
     1021 ttggataaat atgcagaaag ctggaaggaa gtatctgatc cgatatggga agagaactac
     1081 gcaaagaaaa aagaagaggc aaaggtaaaa tagtatggag agggcgaaag aatcagagaa
     1141 aactttccga tatggagagt ggggtcccaa gtacataatg cggggacccc gtattgagtg
     1201 gggattgctc aggttaatac cggggaaaga gcttggagct cattaccata gagaagtaga
     1261 agagacattc tatgtgattt ctggaaaggc acagatgaca gtggatggag aaactttcga
     1321 gatttcagct ggagatgcga taagggtcga agcccctgaa gaacacaata ttattccttc
     1381 ggaggatttt ctggctatct ttataaaggc accctattta cctgaagata aatatacaaa
     1441 atagagaacg catctagatt taggtacgat gaatcattaa gatcaggaag cgtaagataa
     1501 aaaagagggt tcgagatgtc ttgaccctct tttttatctt tagccattaa tttgtgccag
     1561 attctttttc gccatctttt cttgatacat tctcttatcc gcttcttcta tcaggtctct
     1621 cagagaaaca ggactatccg gatcatatac cgccagtcct atacttagag atagagggta
     1681 gcgagatgtt ttcttactat ttctggcatc aagctttctc ctgattcttt ccaccagaat
     1741 accaggatta ttcttctctg tctcaagccc taaaaataca aactcatctc cacctatacg
     1801 ggctaagaca tcagatttcc tgaaagtttt tctaagaata gaagcaactt ctatcagggc
     1861 ttcatctcct gtactgtgac ccagattatc gttaatccat ttcatattat ccacgtccat
     1921 atacataaga atacttcttt tttttaatct tttactgagt cttaggtgct gtcttgctaa
     1981 catcataaaa cctcttctat tatggattcc agtaagttcg tccgtcaggc tcaatcttct
     2041 gagattcttc tccgccgtat ctctccttct gagaatctct actgtgtcaa atacatcctc
     2101 taatctttta gcaaagatag catatatacg cctctcacgg tcatctatag gtttatcctt
     2161 ttctagatag
//
LOCUS       DAOTKU010000292         2167 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_40813_length_2167_cov_3.078598, whole genome shotgun sequence.
ACCESSION   DAOTKU010000292 DAOTKU010000000
VERSION     DAOTKU010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40813_length_2167_cov_3.078598"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="P5310_06800"
     CDS             complement(<1..481)
                     /locus_tag="P5310_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRU33105.1"
                     /translation="MEEKLLLEKTRNIGIVAHIDAGKTTTTERILFYTGKVYKIGEVD
                     EGSATMDWMPQEKERGITISSAVTTCYWREHRINIIDTPGHVDFTAEVERALRVLDGI
                     VAIFCGVAGVQPQSETVWRQASKYKIPRIVYVNKMDRSGANFLRVVNMIRERLSANAV
                     "
     gene            complement(491..961)
                     /gene="rpsG"
                     /locus_tag="P5310_06805"
     CDS             complement(491..961)
                     /gene="rpsG"
                     /locus_tag="P5310_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012676446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HRU33106.1"
                     /translation="MPRKGPVPKRDIPPDLVYQSELVAKFINKIMLDGKKTKAEKIVY
                     SSLKNLEERLGKSGAEILEQAIENVRPLVAVRPRRVGGATYQVPIEVDLEKGRALAIR
                     WIIDAFRNRGGKSSVEKLTAELLDAYNKVGTAIKKREDTHKMAEANRAFSHYRW"
     gene            complement(973..1347)
                     /gene="rpsL"
                     /locus_tag="P5310_06810"
     CDS             complement(973..1347)
                     /gene="rpsL"
                     /locus_tag="P5310_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009103791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HRU33107.1"
                     /translation="MPTINQLVRFGREKVKVKTKAPALQGCPQKRGVCVRVGTMTPKK
                     PNSALRKIARVRLSNGMEVTAYIPGIGHNLQEHSVVLIRGGRVKDLPGIRYHIIRGTL
                     DTAGVENRRQGRSKYGAKRPKS"
     gene            complement(1420..1854)
                     /locus_tag="P5310_06815"
     CDS             complement(1420..1854)
                     /locus_tag="P5310_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 domain-containing protein"
                     /protein_id="HRU33108.1"
                     /translation="MEEKLNQEGKMEEKSNQEVKAEEKPIDKFSQMVGSAAKKLGEGL
                     SQIAQRLGKETSKMARIAGLKADIFRLQNDKRSKLEELGNKVLSLYKEDKLSTDNLEA
                     LKDNINSILEIEKEIDLKNSEIKKIQEEEKMTDEEVSQIPMG"
BASE COUNT          518 a          563 c          354 g          732 t
ORIGIN      
        1 gaactgcgtt agcactaagc ctctccctta tcatattaac tacacgaagg aagttagcac
       61 cagacctgtc cattttattt acatagacta tcctcggaat cttgtattta gaagcctgtc
      121 tccataccgt ctcagattga ggttgaaccc ctgcaacccc acagaatata gcaactattc
      181 catccagtac cctcaaggct ctctctacct cagctgtaaa atccacgtgc ccgggtgtat
      241 caattatatt tatccgatgt tcacgccagt aacatgtggt tactgctgag ctgatggtaa
      301 ttcctctttc cttctcctga ggcatccagt ccatagtggc agaaccttcg tctacttctc
      361 ctatcttata aaccttaccg gtatagaaca gtatcctttc cgtagttgta gtctttcctg
      421 catctatatg tgccactatt cctatattcc ttgtcttctc cagcaataac ttttcctcca
      481 atcttctcac ctaccatcga taatgagaga atgccctgtt agcctcagcc atcttatggg
      541 tatcttcccg cttctttatt gcagttccaa ctttattata tgcatctaag agttcagcgg
      601 taagtttctc caccgaactc ttaccacccc tattcctgaa tgcatcgata atccatctta
      661 tagccagagc tcttcccttt tcaagatcca cttcaatagg aacctgatag gtggctcctc
      721 caacccttct gggtcttacc gctactaaag gtctaacatt ctctatagcc tgctccagaa
      781 tctccgctcc agatttgcct aatctctcct ctaaattctt aagagaagaa tatactatct
      841 tttctgcctt tgtcttcttc ccatccagca ttatcttatt tataaatttt gctaccaatt
      901 cactttgata taccaaatcc ggtggtatat ccctttttgg cacaggtcct ttacgtggca
      961 tctacaaacc tcctaagact ttggtctttt agctccatac ttggaacgac cctgccttct
     1021 attctcgaca cctgcagtat ctaaagtgcc tctaattatg tgataacgaa ttccaggaag
     1081 gtccttgacc cttccccctc gtataaggac taccgagtgt tcctgtagat tatgcccgat
     1141 accgggtatg tatgcagtta cctccatccc attagaaagc cttactctgg caatctttct
     1201 taacgccgaa ttaggcttct taggggtcat agtccctaca cggacacata ccccacgttt
     1261 ctgagggcat ccttgtagtg ctggagcctt tgtttttact tttacttttt ctctaccaaa
     1321 tctaactaac tggttaatag taggcattag tcacctccaa tattacagct gaatatagta
     1381 tcatttttgc agttgtatgt caatagcctg tttatctatt taacccatag ggatctgaga
     1441 gacctcttcg tcggtcatct tctcttcttc ctgtatcttt tttatctcgg aattcttcaa
     1501 atcgatctct ttctctatct ccagtataga gttaatatta tcctttagag cctcaaggtt
     1561 atcggtagat aacttatcct ctttgtaaag agataaaacc ttattcccaa gctcttccag
     1621 cttgctcctt ttatcatttt gcaatctaaa aatatccgcc tttaatccgg ctatgcgtgc
     1681 cattttgctc gtctctttcc caagtctttg tgcaatctgg ctcaaaccct ctccgagctt
     1741 cttggctgcc gaacccacca tctgagaaaa tttatctata ggtttctctt ctgcctttac
     1801 ttcttggtta gatttttctt ccatcttccc ctcttgatta agcttttctt ccatctttcc
     1861 cctcctgatt aatagattta tagtattcta attttatcat aaaccttata caaactcaaa
     1921 tagttgttaa cgttctgtga tattgtcata ggtaaatagt tctgacctat tcccctctcc
     1981 cttgacggaa gttcttcttt ctccctctcc ctcgatggga gaggggaggg gtgagggtga
     2041 aaagtaagat agtgccccca cctctatcct cccccgaggt ggggaggaag aaaagagagc
     2101 gttcccccga ggtgggggag gaaggataaa ggcatggtgt ttttattata aaagacgaaa
     2161 ttgtagg
//
LOCUS       DAOTKU010000293         2165 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_40862_length_2165_cov_4.167773, whole genome shotgun sequence.
ACCESSION   DAOTKU010000293 DAOTKU010000000
VERSION     DAOTKU010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40862_length_2165_cov_4.167773"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..172)
                     /locus_tag="P5310_06820"
     CDS             complement(35..172)
                     /locus_tag="P5310_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU33109.1"
                     /translation="MRQLIMTIPHELDDAAKIEEGRSSLLILLEKECRKLLFYVTSSH
                     H"
     gene            complement(187..1809)
                     /locus_tag="P5310_06825"
     CDS             complement(187..1809)
                     /locus_tag="P5310_06825"
                     /inference="COORDINATES: protein motif:HMM:NF013469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5597 domain-containing protein"
                     /protein_id="HRU33110.1"
                     /translation="MSARTIFSVNGQPFFPLGGQVHNSSAYTASELETGWRALEEIRA
                     NTAEIPIYWDLIEPEEGGFDFSVLGKMIESAREHNLKLILLWFGTWKNGTMKYTPSWV
                     KNNPKKFKRVITREGVSTSVLSSHCDTNLEADTKAFRALMEYIKKVDEKEQTVIALQV
                     ENEPGIMGGAKRDYSKESEKEFRNLVPEYLIRKVQENSGSIINNVWKENGGKDKGSWE
                     ELFGYDAEEFFTAWSIARYIDRIAEGGKKIYDIPMYVNVWLATEWDIPGAGYPSGGAV
                     PKVLDIWKWTAKNIDLIAPDIYARNQKSYYDFCNLYNRNDNPLFIPESSIDESNSLNM
                     FRAIADYNAVGFHIFGIESILDENGSVKSSARSVVNSFKSLSSAIPLILRYQGTSNIH
                     SVIQEEYASEQRINLGKYAGFVRFLQLDPYTHLIDPGAGPLDFHHRDQIREERGRGLL
                     IQTGEREFYLLGGGYRLLLYRKDMLRAPLSVFYSSDFIKSRSIDYLSVEEGFFDADGN
                     WITARKRSGDESDYGVWVHPDVGVVRVVLSEE"
BASE COUNT          634 a          527 c          363 g          641 t
ORIGIN      
        1 taagtcacct cggtgaagga ggaagcccac cactttagtg gtgggaggat gtcacataaa
       61 acaacaattt tctgcactct ttttctaaca atataagcaa ggatgaccgc ccctcctcta
      121 tcttcgctgc atcgtctaac tcatggggaa tagtcataat aagctgtctc atatctcctt
      181 ccgatgttac tcttctgaga gaaccaccct cactaccccc acatcaggat gaacccatac
      241 accgtaatcg ctctcatcgc cgcttctttt cctcgctgta atccaatttc catcagcatc
      301 gaagaaacct tcttcaacag agagataatc tatacttctg gactttataa aatcactgct
      361 atagaagacc gataatgggg ctcttagcat atcttttctg tataataaga gcctgtaccc
      421 tccacctaac agatagaatt ctctctcccc agtctggatc agtaatcctc ttcctctctc
      481 ttctcttatc tggtctctat gatgaaaatc gaggggtcca gcgccagggt caattagatg
      541 tgtgtatggg tctaactgca agaacctgac gaaaccagca tattttccca agtttatcct
      601 ttgctcgctg gcatattcct cctggatcac agaatgtata ttacttgtcc cctgatacct
      661 gagaataaga ggtatagcag aggataagga cttaaagcta ttcactacag atctggctga
      721 agattttacc gaaccattct catctagaat actttcaatg ccaaatatat ggaatccaac
      781 ggcattataa tcagcaatag ctcgaaacat atttaatgaa ttactctcat ctatagatga
      841 ttcagggata aaaagcggat tatcatttcg attatagaga ttacagaaat cataatagct
      901 cttctgatta cgcgcataga tatcgggtgc aatcaggtct atattctttg ctgtccactt
      961 ccatatatcc aggaccttag ggacagctcc tccagaagga taacctgctc cggggatatc
     1021 ccactcagta gctaaccaca cattcacata catagggata tcatagatct tctttcctcc
     1081 ttccgctatt ctgtctatat atctggcgat actccaggcg gtaaagaatt cttcagcatc
     1141 atagccaaat aactcctccc aggagccttt atcttttccc ccattttcct tccatacatt
     1201 attgatgata ctgccggaat tctcctgaac ttttctgatc agatactcag gaaccaggtt
     1261 tcgaaactct ttctccgatt ctttactgta atctctcttt gctccaccca taataccagg
     1321 ttcattctct acctgaagag caataactgt ttgctccttt tcatccacct tctttatata
     1381 ctccatcagt gcacggaaag ccttcgtatc agcctccaaa ttggtatcac aatgagaaga
     1441 aagaacagag gtagacactc cctctctggt gattacacgt ttaaatttct tcggattatt
     1501 ctttacccag gagggtgtat atttcattgt tccattcttc caggtgccaa accagagcag
     1561 tatgagtttt aaattgtgct cccttgcact ctcaatcatc tttcctaaaa cagaaaaatc
     1621 aaaccctcct tcttctggct ctataagatc ccagtatatt ggaatttcgg ctgtattagc
     1681 ccttatctct tctaatgccc tccatcctgt ctctaattca ctggcagtat aggcactgga
     1741 gttatgtacc tgtccaccta atggaaaaaa tggctgaccg ttaacagaaa agatcgtcct
     1801 tgcactcata ctattatcac cttccttttt atcatcaatt atacactact tttgtctata
     1861 ttcccctgta ctttcacagc gctgagctcc aggatgatgc caggcacttg agaacatatc
     1921 tccaagagaa ggtcaaaaat caaaggtcag taacaaaaag ttagaagatg ttacatctaa
     1981 atctaatttc atcccaaaaa cagttaccag aacccaaagg ggtgatgagt aagatcaagg
     2041 gatggttcag aaaagagtaa aacttcatag ctatctgaat gctataatag ataagggaaa
     2101 ggaagcgtac aggtgatagc gaatgaatac taaaaaattg accccagcag ctataagaaa
     2161 actgg
//
LOCUS       DAOTKU010000294         2159 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_41012_length_2159_cov_3.126426, whole genome shotgun sequence.
ACCESSION   DAOTKU010000294 DAOTKU010000000
VERSION     DAOTKU010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41012_length_2159_cov_3.126426"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /gene="gatB"
                     /locus_tag="P5310_06830"
     CDS             complement(<1..623)
                     /gene="gatB"
                     /locus_tag="P5310_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HRU33111.1"
                     /translation="MDYEVNIGMEIHAELLTRSKVFCSCPVQFGAPPNTLVCPVCLGL
                     PGALPVLNKRAVEFAITTALALNCGINRHSIFHRKNYYYPDLPKNYQISQYDSPIGIQ
                     GYLEIETEGGRKRIRIRRVHLEEDTGKLIHSGGRITGSDISFVDFNRSSVPLLEIVSE
                     PDISSPEEAKEYLSNMRDILVFLGVCDGKMEEGSMRCEANISVRRKGS"
     gene            complement(626..2062)
                     /gene="gatA"
                     /locus_tag="P5310_06835"
     CDS             complement(626..2062)
                     /gene="gatA"
                     /locus_tag="P5310_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HRU33112.1"
                     /translation="MTNLADLTAHELLGLISRREVSPVDIVNSCINRIEMYESQVKAF
                     LNIRDAETIINSIREDEPYSAIPIAIKDNMLVKGQPATCASKILSNFIAPYNATVIKK
                     LIQNSFNIIGKTNLDEFAMGSSTENSAFYPTRNPWDLERVPGGSSGGSAASVAIGFSI
                     WSLGSDTGGSIRQPASFCGVVGLKPTYGTVSRYGLVAFASSLDQIGPITRDVTDCALL
                     YSFISGYDPNDSTSLNMPPYKFSPPKGDIKGLKIGLVKELMGEGVDPSVRACMDNSIK
                     IFEDSGAIVEEVSLPHIEYSVPTYYILATAEASSNLARFDGVRYGLRANGKTAEEMMF
                     NTRGEGFGPEVKRRIMLGTYVLSAGYYEAFYLKALKFRNLIADDFKKAFERFDVLISP
                     TSPILPFKLGEKISNPLEMYLSDICTIPVNLAGIPAISIPCGFVNNLPVGLQIIGPTF
                     SESKLFEVSYGFEQATSFHKKRPQIGGS"
BASE COUNT          637 a          497 c          381 g          644 t
ORIGIN      
        1 gaaccctttc tccttactga gatatttgcc tcacatctca tacttccctc ttccatttta
       61 ccgtcgcaga ctccaaggaa caccaagatg tctctcatat ttgagagata ctctttagct
      121 tcttcagggc tggatatatc cggctccgag actatctcaa gaagaggaac actgcttcta
      181 ttaaaatcta caaaggatat atcagaacca gttatacgtc caccactatg gatcagctta
      241 ccagtatctt cctcaagatg gacccttctt atccttatcc tttttcttcc gccttcagtc
      301 tctatctcca gatatccctg tattcctata ggagagtcat actgtgatat ctgataattc
      361 ttgggtaagt caggatagta atagttcttt ctgtggaata tagaatgtct gtttatcccg
      421 caattaagag caagagctgt agtaattgca aattctactg cccttttatt aagtaccggt
      481 aaagctccag gcaatcctaa acatacagga cagaccagtg tattaggagg ggctccaaac
      541 tgaacaggac aactacaaaa gaccttggac ctggttaata actctgcatg aatctccata
      601 cctatattga cttcataatc cataactaac tccctcctat ctgaggtctc ttcttatgaa
      661 aagatgttgc ctgttcaaat ccataagaca cctcgaagag tttcgattca ctgaaagtag
      721 gacctataat ctgcagacct acagggagat tattgacaaa tccacaggga atagatatcg
      781 ccggaattcc agccagattc accgggattg tgcatatatc ggatagatac atctccagag
      841 gattggatat cttttctccc aatttaaatg gcaatattgg agatgtcgga gaaataagca
      901 cgtcaaatcg ttcaaatgcc tttttaaaat catcagcaat aagatttcta aacttcaaag
      961 ccttcagata aaatgcctca tagtaaccag cactcaagac atatgtccct aacattatcc
     1021 ttctctttac ctctggtcca aaaccttcac cccgtgtatt aaacatcatc tcttctgccg
     1081 tctttccatt cgctcttaat ccatacctca caccgtcaaa cctggccaga ttagaacttg
     1141 cctccgcagt agcaagtata tagtaagtag gtacagagta ttctatgtga ggtaaagaaa
     1201 cttcttctac tatagcacct gaatcttcaa atatctttat tgaattgtcc atacaagctc
     1261 ttacagaagg gtctacacct tctcccataa gttctttaac cagacctatc tttaacccct
     1321 ttatatctcc tttgggtgga gaaaatttat atgggggcat attgagagaa gtagaatcat
     1381 tcggatcata cccgcttata aagctataca agagggcaca atctgtcaca tctcttgtaa
     1441 taggacctat ctggtcgaga gaagaggcga atgcaaccag tccatatctg gatacggtac
     1501 cgtatgtggg ttttaagcca accaccccac agaaagatgc tggctgtctt atggaaccgc
     1561 ctgtatctga acctaaagac catatagaaa accctatagc cacacttgca gcagaacctc
     1621 cactggagcc tcctggaacc ctctccaagt cccagggatt tctggtagga taaaatgcag
     1681 agttttctgt agaagagccc atagcaaatt catctagatt agtcttacct ataatattaa
     1741 aagagttttg aattaatttt tttataactg tcgcattgta tggggcaata aaatttgaaa
     1801 gtatttttga ggcacaggta gcaggttgcc cttttaccag catattatct tttatggcta
     1861 taggaatggc ggagtatggc tcatcctctc ttatagaatt aataatagtc tcagcgtctc
     1921 tgatattgag aaatgcctta acctgagatt catacatttc tattctattt atgcagctat
     1981 tgactatatc taccggggaa acttccctcc tggagattaa ccccagaagt tcatgagctg
     2041 taagatctgc cagatttgtc atccttcacc tcccaggatt ggaggtataa caaagaaacc
     2101 accatcctct gaaggtgcat tacttaacgc ttcttcttgg gtaagattct cttgaggga
//
LOCUS       DAOTKU010000295         2157 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_41066_length_2157_cov_3.321123, whole genome shotgun sequence.
ACCESSION   DAOTKU010000295 DAOTKU010000000
VERSION     DAOTKU010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41066_length_2157_cov_3.321123"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1267
                     /gene="larA"
                     /locus_tag="P5310_06840"
     CDS             <1..1267
                     /gene="larA"
                     /locus_tag="P5310_06840"
                     /inference="COORDINATES: protein motif:HMM:NF033504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HRU33113.1"
                     /translation="IRLPYGDGTREFNISGLKPLKILKPKDYSGTNNYRDLIQGAIEK
                     PMYGPSLKDIARGKSSAVILVSDKTRRTLAKEIIPDILEELSQCGIPLSRIAVIIAVG
                     AHPMLTEEEIVSLLGDDVAGSVTVVNHNCREDFSLEYKGLSSRGTPIHINKTLLEADL
                     KILTGSINYHDFAGFSGGAKSILPGISGLSTISHNHLLLLHPVFGAGYNPMAKAGILD
                     GNPVHEDMEEIAELVNPDFLINVVFNAAGELLQVVSGNWRLAHRMGCKTIEDIFSADI
                     EEKADLVLVSAGGYPFDINFYQVMKSVINTMGMIKENGTIILVASCKEGLGAEHFKEW
                     LSIESPLKLEEELRKDFNMIGKIAYDIRVGLKGKRVLMISDLPGEDVELLGFKNANSL
                     EEAKEMLALKDEATMYIVPYGNITVAKTS"
     gene            complement(1365..>2157)
                     /locus_tag="P5310_06845"
     CDS             complement(1365..>2157)
                     /locus_tag="P5310_06845"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRU33114.1"
                     /translation="SMDQYLDFPVKDKRSFEELKKGYNPYSPVRYPMWWDELAKLWRD
                     RDYPLCLFVNGQIGFYWTLREWVGTENLSYMFYDDPSLVHEMLDFIADFIINVSTRAL
                     SDVQIDYFNFSEDLAGKSGPLISPKIYKEFFVPRVRRVIEHFKKFRVRYFWVDSDGNF
                     EVLIPYMLEAGINCIWPLEQASGMDPVKLRKRYGKDLVLCGGIDKRELTKDKASIERE
                     LYSKIPYLLEQGGYIPHLDHTFPPDISYENFLYYMELKEKLLRGG"
BASE COUNT          670 a          415 c          451 g          621 t
ORIGIN      
        1 aataagatta ccatatggag atggaacaag agagtttaat atttcaggat tgaagccttt
       61 aaagatactg aagcctaaag attattcggg gacaaataat tacagggatc ttattcaggg
      121 ggctatagaa aagccaatgt atggtccatc tcttaaggat attgccaggg gtaaaagctc
      181 tgcagttatt ctagtaagtg ataaaacaag gaggactcta gcaaaagaga ttatcccgga
      241 tatcctggag gaactttctc aatgtgggat acctctcagc aggatagctg taatcattgc
      301 agtaggggct catccgatgc ttacagagga ggagatagta tctcttttag gagatgatgt
      361 ggcaggcagt gttaccgttg tgaatcataa ctgtagagaa gacttttccc tggagtataa
      421 aggactctct tctagaggga ctcctatcca tataaacaag actcttctgg aggctgactt
      481 aaagatacta acaggctcga taaactacca tgactttgct ggctttagcg gaggggcaaa
      541 atctatactc cccgggatat caggactctc taccataagc cataatcatc ttcttctttt
      601 acaccctgtt ttcggagctg gatataatcc catggctaag gctggtattc tggatggaaa
      661 ccctgttcat gaggatatgg aagagattgc tgagctggta aatcctgatt ttcttataaa
      721 tgtggtattc aatgccgccg gagaactcct acaggtagtc tctggtaatt ggagattggc
      781 tcatcggatg ggatgtaaaa ctatagagga tatattctca gcagacatag aagaaaaggc
      841 tgacctggta ttggtgagtg caggggggta cccattcgat atcaacttct atcaggtaat
      901 gaagtccgta ataaatacca tggggatgat aaaagaaaat gggacgataa tcctggtcgc
      961 ttcgtgtaaa gaagggcttg gtgcggaaca ttttaaagaa tggcttagta tagaatctcc
     1021 actgaagtta gaggaagaat tgagaaaaga tttcaatatg atagggaaga tagcttatga
     1081 tataagggtt gggctaaagg ggaaaagagt cctgatgata agcgatcttc ctggagaaga
     1141 tgtagaactt cttggattta agaatgctaa cagtttagag gaggcaaagg agatgttagc
     1201 tctaaaagat gaggccacca tgtatatagt gccatacggt aatattaccg tcgccaaaac
     1261 atcctaattt tgtgatcacc cgccgggaaa acccaccctt ttaaaggtgg gatgaaaggc
     1321 gggtgataag tagtgtttct tccgggaagt tattttatcc ctttttagcc tccccgtaaa
     1381 agtttttctt tcaactccat atagtataaa aaattctcat acgatatatc aggtgggaat
     1441 gtatggtcca gatgcgggat atagccaccc tgctccagaa gatatggaat tttactgtaa
     1501 agttctcttt ctatagacgc tttatcctta gttagctctc tcttgtctat gccaccacac
     1561 agtaccaggt ccttcccata tctttttctt agctttaccg ggtccatacc tgatgcttgt
     1621 tcaagaggcc agatacaatt aatccctgcc tctaacatat atgggatcaa gacttcaaag
     1681 ttaccatcac tatccaccca gaagtatctc actctaaatt ttttaaaatg ttctatgact
     1741 cttctcactc tgggaacaaa aaattcttta tatatcttag gagatattag tggtcctgat
     1801 ttaccagcaa gatcctcaga aaaattaaaa taatctatct gtacatcaga taaggctctg
     1861 gtactcacat ttattataaa gtcggcaata aaatccagca tctcatgaac caaagatgga
     1921 tcatcataga acatatacga tagattttct gtcccaaccc actccctcaa tgtccagtaa
     1981 aaccctatct gaccattcac aaaaagacac aaaggataat ctctatctct ccataacttt
     2041 gccagttcat cccaccacat aggatatctt acaggagaat atgggttgta acctttctta
     2101 agctcttcaa aacttctttt gtctttaacc ggaaaatcga gatattgatc cattgaa
//
LOCUS       DAOTKU010000296         2155 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_41115_length_2155_cov_3.291905, whole genome shotgun sequence.
ACCESSION   DAOTKU010000296 DAOTKU010000000
VERSION     DAOTKU010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41115_length_2155_cov_3.291905"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="P5310_06850"
     CDS             complement(<1..265)
                     /locus_tag="P5310_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HRU33115.1"
                     /translation="MKKVCILLIFLLFLSLSLGEIKANDLWRYDLEKAISFSLKEENL
                     SGISELAKSLKGATIQESAWNILEWEEKNIEYDFDKAQLPEPVI"
     gene            479..805
                     /locus_tag="P5310_06855"
     CDS             479..805
                     /locus_tag="P5310_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRU33116.1"
                     /translation="MEECRLIGEPVSLKELVNYQEGAVVSRTIIDKKTGTVTLFAFDK
                     EQGLSEHTAPFDALAYIIEGEVDVIISGKSYQLKEGEMIIMPANQPHALRAVERFKMI
                     LIMIRD"
     gene            881..1432
                     /locus_tag="P5310_06860"
     CDS             881..1432
                     /locus_tag="P5310_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU33117.1"
                     /translation="MKFISVFLVILISLVSTASTVASGEIIDITGRFIGKDNYLLVSV
                     KNNVYNVKGKIPEGLKENDYLEVKGIERKDENGNSYIWALRIRVLLPVTISSLKEGVK
                     DYLNKKVLIYGEFRGWEGTAGPPPVTRSDWLIKDDTGYIYVLGAPPFKPGEDKGKKIA
                     LVGVLKLSRENIPYVEPLLVDVR"
     gene            1455..>2155
                     /locus_tag="P5310_06865"
     CDS             1455..>2155
                     /locus_tag="P5310_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HRU33118.1"
                     /translation="MEFLGNLTPALQALIATLFTWGVTALGSAGVFLAKDVNRRVLDG
                     TLGFSSGVMIAASYWSLLAPSIEMSRELNLIEWIPAVTGFLLGGLCLYIIDKTLPHLH
                     MDLPTKDAEGIKTTWKRTTLLVLAITLHNIPEGLAVGVAFGASALGLPSASLPSAIAL
                     AIGIGLQNFPEGLAVSMPLRREGLSPFKSFWYGQLSGMVEPVAGVIGASVVTIAKPIL
                     PYALAFAAGAMIYVVV"
BASE COUNT          656 a          370 c          501 g          628 t
ORIGIN      
        1 ggattacagg ttcaggtaac tgggctttat cgaagtcata ctcaatattt ttctcttccc
       61 attccaggat attccaggca ctctcctgaa tagtagcccc ctttaaactc tttgccagct
      121 cagaaatacc agaaaggttc tcctctttta aggaaaaaga aatagccttc tctaaatcat
      181 atctccagag atcatttgcc tttatttccc ccagagataa agaaaggaat aaaagaaaaa
      241 ttaagagaat acatactttt ttcatagtta tcctccagct aatgttaagt gatagataat
      301 aataattcta ctacttatct ctcttcttta cgagactttt ctgaagcttg tgatatctta
      361 aggtaagatg atataatcaa tatatgcccg agaataaaga ggaaagtctt gtggtgtctt
      421 taaaaaatag acttcaaatc cggaacgtat aattcagctg gtacaggagg ttagtattat
      481 ggaagagtgt agattaattg gtgagcctgt cagcttaaaa gagctggtaa attatcagga
      541 aggggctgtg gtgagtcgaa caataatcga taagaagact ggtacggtaa cattatttgc
      601 atttgataaa gagcagggat tgagtgagca tacagcccca tttgatgccc tggcttacat
      661 tattgaaggt gaagtagatg ttatcatctc tggtaaatct tatcagctga aagaaggaga
      721 gatgataata atgccagcca atcaaccaca cgctttaaga gcggtggaga gatttaaaat
      781 gattttaata atgatacggg attaaacagg gaatgatttt tcatttgtaa ttcttaaacc
      841 tttgtggtat attttagaca aaaaggtagg tggtggtttt atgaaattta tcagtgtatt
      901 tttagtaata cttatttccc tggtcagtac tgcaagcacc gtagcctctg gagagattat
      961 agatataacg gggagattta tagggaagga taactatctc ctggtatctg taaaaaataa
     1021 tgtgtataac gtaaagggga agattcctga aggacttaaa gagaacgatt atctggaagt
     1081 aaaaggaata gagagaaaag atgaaaatgg aaattcgtat atctgggctt tacggataag
     1141 agtcctctta cctgttacta tctcttctct taaagagggg gtcaaagatt atctcaataa
     1201 aaaggtcctt atctacggag agtttagagg ctgggaagga acagcaggtc cacccccagt
     1261 aaccaggagt gattggttga taaaagatga tactggatac atatatgtcc ttggagcacc
     1321 tccatttaag ccaggagagg ataagggtaa aaagatagcc ctagtaggag tattaaagct
     1381 atccagagag aatatcccct atgtagaacc cttgctggta gatgtaagat aaaattggat
     1441 ttgggggata agagatggag tttctcggaa atctgacacc tgcccttcaa gcattaatag
     1501 ccacactgtt tacctggggg gttactgctc ttggttctgc cggggtattt ctggcaaagg
     1561 atgtaaatag aagggtcctg gatgggacac tggggttttc aagcggggtg atgattgcag
     1621 caagttactg gtctttatta gctccttcta tcgaaatgtc gagagaactg aatcttattg
     1681 aatggatacc tgcagtaaca ggttttctcc tgggaggatt atgcctgtac atcatagata
     1741 aaacattacc acatctacat atggatcttc ccacaaaaga tgcagaagga attaagacca
     1801 cctggaagag gacaactctt cttgtcctgg ctatcacgct ccacaatata ccggaaggac
     1861 ttgcggtagg tgtagctttt ggtgcatctg ctttaggtct tccgtctgcc tctttaccct
     1921 ctgcgatagc tctggctatt ggtatcggac ttcagaattt tccagaaggg ttagccgttt
     1981 ctatgccatt gaggagggag ggactatccc cttttaagag tttctggtac ggacagttat
     2041 cggggatggt tgagccggtg gcaggagtaa taggagccag tgttgtcacc atagccaaac
     2101 ccatattacc atacgcgctg gcatttgcag ctggagctat gatatatgtg gtggt
//
LOCUS       DAOTKU010000297         2144 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_41402_length_2144_cov_1.977980, whole genome shotgun sequence.
ACCESSION   DAOTKU010000297 DAOTKU010000000
VERSION     DAOTKU010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41402_length_2144_cov_1.977980"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1333
                     /locus_tag="P5310_06870"
     CDS             <1..1333
                     /locus_tag="P5310_06870"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GumC family protein"
                     /protein_id="HRU33119.1"
                     /translation="KRRIYIITLFILAVFGAYLFSKVTPKTYSAKAVIILPSEKGGLS
                     TQLLLQSLPVVGDITGLTGGIVANYTAILKSRSAAEYVVKNLNLGEKYKADNLQRATD
                     ILQKSVKISDTKENTIEISAEAKDPELAADIANTYISALRDITSKLILSSAQREKSFL
                     EERLEETEKLLREAETNLKAFQDRHKMVSIDEETKVLVENIANLQAQKESTEIELNTL
                     NTQTNNLQSLLTSQAELLGKDPLTVTSISSDPKIQVWREKLIEDEVALATLLQDYGDL
                     HPKVVEIKQEIAEINRVMREEIERLSKALDTLSTSELFDLNVKKISLEAKLQGLDRLI
                     QEYDARLSKLPELSLEFSRLLRDLKLQEAIYTTLATQYEQAKISEARETIEIQVLDYA
                     VPPEKHSKPNTMLNVLIGGVAALFLGIFLAFFLEFWQNLKGELKKDEDSSL"
     gene            1314..>2144
                     /locus_tag="P5310_06875"
     CDS             1314..>2144
                     /locus_tag="P5310_06875"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRU33120.1"
                     /translation="MKIPVFDLKRQYQMIKPEVNGVIQSVLEGGQFILGENVSLLEEE
                     IANYCGVKHAIGVASGTDALLLSLRACGIGPEDEVITTPFTFFATAEVISNLGAKPVF
                     VDIEPETFNIDIEKIEKSITKKTRAIIPVHLFGQICDMDQIMNLKEKYNLFIIEDACQ
                     AIGAVYKDKKSGSMGNTGCFSFFPTKNLGGYGDGGMITTDSDELKEKLFLLRGHGSKK
                     KYYHDELGYNSRLDELQAAILRVKLRYLDTWISRRQELANLYTSLLSDIPDVITPVVK
                     G"
BASE COUNT          718 a          341 c          490 g          595 t
ORIGIN      
        1 gaagagaagg atttatatta taactctctt tatactcgct gtatttggag cctatctctt
       61 cagtaaagta acccccaaga catattctgc taaagcagtt attattttgc ccagcgaaaa
      121 agggggattg agtactcagc tgttactcca atctttacca gtggtaggag atataacagg
      181 attgacgggg gggatagtgg caaactacac tgcaatactg aaaagtcgtt ctgctgccga
      241 atatgtagtt aaaaatttaa atctaggaga gaaatataaa gctgacaatc tacagagagc
      301 tactgatatt cttcaaaaat cggtaaaaat aagcgataca aaagagaata ccatagagat
      361 atctgcagaa gcaaaagacc cagaattggc agctgatata gctaatactt atatttctgc
      421 ccttagagac attacttcca agctcatatt aagttcagct caaagggaga aatccttcct
      481 ggaagaaaga ctggaagaaa ctgaaaagct tttaagggag gcagagacca atcttaaggc
      541 ttttcaggat agacataaga tggtcagcat tgatgaagaa acaaaagtct tggttgagaa
      601 tatagctaat ctgcaggctc aaaaggaatc tactgagata gaactgaata cattaaatac
      661 ccagacaaat aatttacaat ctttgcttac ctctcaggct gaattgcttg gtaaggaccc
      721 acttacggta acttctataa gttcagaccc aaagattcag gtatggcgag aaaaacttat
      781 agaggatgaa gttgctcttg ccactcttct tcaggattat ggagacctgc atccgaaagt
      841 ggtggaaata aaacaggaga tagcagagat aaatagagtt atgagggaag agattgaaag
      901 attatcaaag gctctggaca cactttctac atctgagcta tttgacctga atgtgaagaa
      961 gatatctttg gaagctaaat tgcagggttt agacaggtta attcaggagt acgatgccag
     1021 gttaagtaag cttcctgagt taagcctaga attctccaga ttgcttagag atttaaagtt
     1081 acaggaagcc atatatacga cccttgccac ccagtatgag caggcgaaga taagcgaagc
     1141 gagagagact attgaaattc aggttttgga ctatgctgta ccaccggaga aacatagcaa
     1201 gccaaatact atgcttaatg tgttgatagg aggagtggct gctctgtttc tgggaatatt
     1261 tttggctttc tttttagagt tctggcaaaa tcttaaaggg gagttgaaga aagatgaaga
     1321 ttccagtctt tgatcttaaa agacagtatc agatgattaa accggaggta aatggagtaa
     1381 tacagagtgt actggaagga ggacagttta ttctcggaga aaatgtgagt ctcctggagg
     1441 aagaaattgc taattattgt ggagttaagc atgctatagg tgttgcatcc gggactgatg
     1501 cactgttact ctctttgaga gcatgcggga taggaccgga agatgaagtt ataaccactc
     1561 catttacttt ttttgctaca gcagaggtta tttctaacct tggtgcaaaa ccggtatttg
     1621 ttgatataga accagaaacc tttaatatag atatcgaaaa gatagagaaa tctattacta
     1681 aaaagacaag ggctattatc cctgttcatc tttttggaca gatatgcgat atggatcaaa
     1741 ttatgaatct taaagagaag tataatcttt ttattataga agacgcctgt caggctattg
     1801 gtgcagttta taaagataag aagtcgggta gcatgggaaa taccggttgc ttcagctttt
     1861 tccctaccaa gaatcttggt ggttatgggg atggagggat gataactact gatagcgatg
     1921 aattgaaaga aaaattgttc ctgttaagag ggcatggaag taagaagaag tattatcatg
     1981 atgaattagg ttacaatagc agacttgatg agcttcaagc agcgatatta agggtgaagc
     2041 tcagatatct tgatacatgg atatccagaa ggcaagagct ggctaatctg tacacatctt
     2101 tactttccga tattcctgat gtcattactc cggtagttaa agga
//
LOCUS       DAOTKU010000298         2143 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_41417_length_2143_cov_3.288314, whole genome shotgun sequence.
ACCESSION   DAOTKU010000298 DAOTKU010000000
VERSION     DAOTKU010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41417_length_2143_cov_3.288314"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="P5310_06880"
     CDS             complement(<1..543)
                     /locus_tag="P5310_06880"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRU33121.1"
                     /translation="MAVNWQGGYSWVRSNAEVVKMKAMAGELSLQTFHFSLTDYPLLK
                     EGEKKGDYRVLLWPSTGADAVLMINHTYNEDPVIAKLLNDPRFKRALSLAINREEINQ
                     LYALGLGRATQYSPAVADPVYEKRYSEAYAKFDLASANKLLDSSGLTKRDRDGYRLRP
                     DGKRLAISVLVATDWPMHSDI"
     gene            1295..>2143
                     /locus_tag="P5310_06885"
     CDS             1295..>2143
                     /locus_tag="P5310_06885"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRU33122.1"
                     /translation="MPKDPSKRFDQLMPYVFSTQDAKKLKLRAEGKRIIDLGMGSPDL
                     PTPRHIVEALVEAVSSRTETHRYPNSKGLPQLRESIAQWYKRRFNVDLDPEGEVFPLV
                     GSKEGIAHLALAYIDSGDFTITLNPTYPAHYNGTLIAGGRVFFLPLREGNDFTPDLTE
                     IPTSILNKTKLMIVSFPHNPTTGTVDIEFYKNVLELAEKYDFIVVSDLAYSEICFDGY
                     RAPSLLEVDGAKERAVEFNTFSKTFNMAGWRTGYVVGNRDVISKLAQIKSYIDYGIFE
                     AIQYACI"
BASE COUNT          668 a          420 c          426 g          629 t
ORIGIN      
        1 gatatcagaa tgcataggcc agtcggtagc tacaagaaca gaaattgcaa gtctcttccc
       61 atcaggtctc aatctataac catctctatc tcttttggtc aatccactag aatcgagaag
      121 cttattcgca gaggctagat cgaattttgc ataagcctct gagtatcttt tctcatatac
      181 aggatctgct actgcaggtg aatattgagt tgctctacca agaccaaggg cataaagctg
      241 gttgatttcc tctctattga tagctaaaga tagtgccctc ttgaatcttg ggtcatttag
      301 aagtttggct ataaccgggt cttcattata ggtatgattt atcataagaa ctgcatctgc
      361 acctgtagat ggccagagca gaactctata gtcacccttc ttttctccct cctttaatag
      421 tggataatcg gtcagggaga aatggaatgt ctgaagggag agctccccag ccatagcttt
      481 catctttaca acctcggcat tggacctgac ccaggaatat cccccctgcc agttgactgc
      541 caatagatat tgggataact atatcagtta atccacagtg acaggtatca attaaaaaat
      601 ctttcccgct gttagatgcc gatattaggc gattatgttc tactcttata cagtcctgat
      661 gcctcttttt agagagttta gtaagcatac atatcggaga attgtgactt ctaaaactta
      721 aatcgatata cagatcatcg accttgtaag taaaataagt cagatcatag atgcatatct
      781 ccatatcact tactgtttgg atggattcta tataatcctg aaagtcagtt ttaatctcac
      841 tcatataaca gtattctaga ttaaatccta tttatattca actaaagtgt caatagtcaa
      901 agaaagtgtc atctatatga aattgtaata cttactaata gtatagatac aaattcggag
      961 ataacctgct ctatcctctc ctatctgagg aagcaactaa ttcttcctcc cccatctcgg
     1021 gggaggatgg aggtgggggc atctcttatt ttcaccctca cccctgccct ctcccgtcaa
     1081 gggagaggga aatactcctg actcatagaa gccagaaata gcttatcatc tatgttctca
     1141 tctttcatgt gtgacattat cacagaacat taacaccaga gttgcataaa gaataactcc
     1201 aacatattga taattcgctt ccatactcct tgacataaat aatagttcat tgttatatat
     1261 agaaaattac attaaaaagg agaggaataa taatatgcca aaagaccctt caaaaagatt
     1321 tgaccagttg atgccatatg ttttttctac gcaggatgca aagaaattaa aactaagagc
     1381 agaggggaaa aggataatag atctgggaat gggaagtcct gatctcccaa cccccagaca
     1441 tatagtggag gctttagtcg aagctgtatc aagtagaacg gagactcata gatatccaaa
     1501 tagtaagggc ttaccccaat tgagagagag tatagctcaa tggtataaga gaagatttaa
     1561 tgtagacctt gaccctgaag gagaggtctt ccctttagtt ggttctaaag aagggatagc
     1621 tcatcttgcc ctggcttata tagactcagg agatttcacc attacattaa atcccaccta
     1681 tccagctcat tacaatggaa ctcttatagc cggaggtagg gtattcttct taccactgag
     1741 agagggaaat gattttactc cggatctaac cgagatcccc accagtatat taaacaagac
     1801 taaacttatg atagtaagtt ttccccataa tcccactaca ggtacagtgg atatagaatt
     1861 ctataagaat gtgctggagc tggcagaaaa gtatgacttt atagtggtaa gtgatctggc
     1921 atattcagag atctgttttg atggctatag ggctccaagt ttattagagg tggatggggc
     1981 taaagagcgt gcggtagagt ttaatacttt ctccaaaaca tttaatatgg cagggtggag
     2041 gacagggtat gtggtcggta atagagatgt catctccaaa ctggcacaga taaagagtta
     2101 tatagattac ggtatatttg aggctattca gtatgcctgt ata
//
LOCUS       DAOTKU010000299         2135 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_41625_length_2135_cov_2.029808, whole genome shotgun sequence.
ACCESSION   DAOTKU010000299 DAOTKU010000000
VERSION     DAOTKU010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41625_length_2135_cov_2.029808"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /gene="fliG"
                     /locus_tag="P5310_06890"
     CDS             <1..822
                     /gene="fliG"
                     /locus_tag="P5310_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HRU33123.1"
                     /translation="QMAIAQDYIAQGGLEYARQVLEKALGTQKALEILERLTATLQIT
                     PFEFMRKMDAKQILSFIQDEHPQTIALILSYLTPEQSAIILSSLPPELMTEVAMRVAT
                     IDRTSPDVVHKVEDILEKKLALLATQESTNTGGIKHFVEILNRVDRNTEKSVLEYLES
                     EDPELADEVKKQMFLFEDIVMLDDRSVQRILREVDSKDLALALKGAGEDVKQKIFKNM
                     SSRASQMLKEDMEFMGPVRVRDVEQAQQKIVSIIRRLDEAGEIVISRGGEEELIV"
     gene            815..1495
                     /locus_tag="P5310_06895"
     CDS             815..1495
                     /locus_tag="P5310_06895"
                     /inference="COORDINATES: protein motif:HMM:NF014196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HRU33124.1"
                     /translation="MSEDKITDLSEFKEVNDQENDSFFLEIQDIVSNVLERSRKTAEL
                     IKTKAREEGYQDGFEQGKVDGYEKGYEEGYKEGYDKGIEELLERVEYLKALGEEILSE
                     RHRLFEEYKYEMVQMVLEISKRLIFEELLTRPEITSNIINNAISIMKEKESIRIIVSP
                     TIFSLIMDRRLSDISGSKVELVSDERLDDGDIIVVSPGGQVEFRLKERFKEIDESYRD
                     VFLNSLRP"
     gene            1452..>2135
                     /locus_tag="P5310_06900"
     CDS             1452..>2135
                     /locus_tag="P5310_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017750834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscN/YscN/HrcN family type III secretion system
                     ATPase"
                     /protein_id="HRU33125.1"
                     /translation="MRAIGMSSLIALDLSRYKEKIDRIRPFKFIGFIDMVSGLTVESI
                     GPVASVGELCRIYSESGEEFFAEVIGFRQNRLILMPIGKTSGIKPGLRVSPLGRSLSI
                     KVNPDILGRIFDALGNPIDDGNSILEGVEYSLDNTPPHPLKRARITDILPVGVKAIDS
                     LLTCGRGQRIGIFAGSGVGKSTLLGMIARHSKADVNVIALIGERGREVRDFIEESLGE
                     EGLRRSVVVV"
BASE COUNT          709 a          299 c          536 g          591 t
ORIGIN      
        1 caaatggcta ttgctcaaga ttatatagct cagggaggat tggagtatgc caggcaagtt
       61 ctggagaagg ctttaggaac tcaaaaagca cttgaaatac tggaaagact taccgctacc
      121 cttcagatta ctccattcga atttatgagg aagatggacg caaaacagat attgagcttt
      181 attcaggatg aacatcccca gaccatagca cttatacttt cttatcttac tccagagcaa
      241 tcagctatca tactctcttc actacctcca gaacttatga ctgaggttgc tatgagggtg
      301 gctactatag acagaaccag tcccgatgtt gttcataaag tagaagatat tttagagaaa
      361 aagttggctc tacttgctac ccaagagtct actaataccg gagggataaa acattttgta
      421 gagattctta atagagtaga tagaaatact gaaaagtcag tattggaata tctggagagt
      481 gaagaccctg aactggcaga cgaggtaaag aaacagatgt tcctctttga ggatatagtg
      541 atgctggatg accgttctgt gcagaggata ctcagggaag tagattctaa agatcttgct
      601 ctagccctta aaggagctgg agaggatgta aaacagaaga tatttaagaa tatgtcctcc
      661 agggcatccc agatgcttaa agaagatatg gaatttatgg gtccagttag agttagggat
      721 gtggaacagg cacaacaaaa gattgtttct ataataagaa ggttggatga agctggagag
      781 attgtaattt ctagaggagg agaggaagag ttaattgtct gaagataaga taactgattt
      841 atctgagttt aaagaggtta atgatcagga gaatgactct ttcttcctgg agatacagga
      901 tatagtttct aatgtcctgg agaggtcaag gaagacagca gagctgataa agactaaagc
      961 cagagaagaa ggttaccagg atggttttga gcaaggaaaa gtagatggat atgagaaggg
     1021 ttatgaagaa ggatataaag aaggttatga taaagggatc gaagagcttt tggagagggt
     1081 agaatatcta aaagctcttg gggaagagat actatctgag aggcacaggt tatttgagga
     1141 gtataagtat gagatggtac agatggtgct ggaaatatct aaaagattaa tatttgaaga
     1201 acttttaacc agacctgaga taacatccaa cataatcaat aatgctatct caattatgaa
     1261 agagaaggaa tctataagaa taatagtaag tccaactatt tttagtttga taatggatag
     1321 aagactatca gacattagtg ggagtaaagt tgagttagta agtgacgaaa gattggatga
     1381 tggagatata atcgttgtat ctcctggagg acaggtagag tttaggctaa aagagagatt
     1441 caaagagata gatgagagct atagggatgt cttccttaat agccttagac cttagtagat
     1501 ataaagagaa gattgatcgt ataagaccgt ttaagtttat aggttttata gatatggtat
     1561 ctggtcttac ggtagaatcc atcggtcctg ttgcctctgt tggagagctc tgccggatat
     1621 attctgagag tggagaggaa ttttttgccg aagttatagg ttttagacag aatagactta
     1681 tacttatgcc tataggtaag accagtggta taaaacccgg actgagagtt tctcccctag
     1741 gtagatctct ctccataaaa gtaaatcctg atattctggg caggatattt gatgccttgg
     1801 gaaaccctat agatgatggt aactctatac tggaaggagt agaatactct ctggacaata
     1861 cacctcctca tcctcttaaa agagccagga taacagatat tttacctgtt ggtgtaaagg
     1921 ctatagactc actgttaacc tgtggtaggg gacagcgtat tggtatattt gctggaagtg
     1981 gtgtgggaaa gagtactctt ttggggatga tagcaagaca cagtaaagct gatgtgaatg
     2041 taattgctct tatcggggaa agaggaagag aggtaagaga ctttattgaa gagtctcttg
     2101 gagaagaagg tcttagaagg tctgttgtag ttgtt
//
LOCUS       DAOTKU010000300         2131 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_41743_length_2131_cov_2.834778, whole genome shotgun sequence.
ACCESSION   DAOTKU010000300 DAOTKU010000000
VERSION     DAOTKU010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41743_length_2131_cov_2.834778"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="P5310_06905"
     CDS             <1..441
                     /locus_tag="P5310_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HRU33126.1"
                     /translation="LLVLEPGRYIVGNAGVTVYTVGAIKEIPGIRKYASVDGGMADNP
                     RPMLYQAQYTAFLANKMREHPRDVFTIAGRFCESGDILIDKAYLPRLERGDILVVSAT
                     GAYNYSMSSNYNMVPRPAVIGVKDGKEDIWVEREDYNDLVRLHR"
     gene            438..1739
                     /locus_tag="P5310_06910"
     CDS             438..1739
                     /locus_tag="P5310_06910"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HRU33127.1"
                     /translation="MNSVNIGMIGLGNVGSGVWRVLEKKKEEANSFPWNVLEIKKIGV
                     RDVRKERQVKVPASLLTQDIDSIINSPHIDVIVEVAGGRDEGFKIISRAINAGKHVVT
                     ANKDVIASYGLELFRLARSKGVKIYFEASVGAGIPIIRVLREDLVANSIQEIRAILNG
                     TSNYILTRMSENGEDFGTALGMAQRLGYAEPNPDNDVKGVDAAYKITILSSIAFNTSV
                     SPDDIYVEGIDTVSIRDIEYAHELGYEIKSLAIARKHNDGLELRVHPTMIPRTSMLAA
                     VRGVYNGIYIVGDAVGPMMFYGRGAGSLPTASAVVSDLCDLVRSLRDGSGGQLEVPAL
                     DNIPIRPIEKLETRYYIRLNVPDRPGVLAGIAMVFGNLGISIASVVQKEVPDTKGQAE
                     LVIITHTAQEGPFRQAISQMDRLDVVNKVESTLRIEGVVEE"
     gene            1736..>2131
                     /locus_tag="P5310_06915"
     CDS             1736..>2131
                     /locus_tag="P5310_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRU33128.1"
                     /translation="MRWNGVLREYKDYLPVTDKTPLITLYEGNTPLIYARNISKELGL
                     KVYLKYEGMNPTGSFKDRGMVVAIAKAVEEGARAVICASTGNTSASASAYSAIAGLKS
                     VVLIPEGAIALGKLAQALTYGAQVIAVKGN"
BASE COUNT          637 a          345 c          572 g          577 t
ORIGIN      
        1 ttactggtgc tcgagccagg gagatatatt gttggtaatg ctggagttac tgtttatacg
       61 gtgggagcta taaaggaaat tccagggata cgtaaatatg caagtgttga tggaggtatg
      121 gctgataatc ccagaccaat gctgtatcag gctcagtata ctgcattttt ggcgaacaag
      181 atgagagaac atcccagaga tgtttttact atagccggaa ggttctgtga atctggtgat
      241 atacttatag ataaggcata tcttcctcga ttagaacgtg gagatatact tgtagtttct
      301 gctaccggtg cctataacta ttctatgtca agtaattata atatggtacc cagacctgca
      361 gtgatcggtg taaaagatgg caaagaggat atctgggtag agagagaaga ctataatgat
      421 ctagtgaggt tgcatagatg aatagtgtaa atattgggat gatagggctt ggtaatgttg
      481 gaagtggagt ttggagggta ttagaaaaaa agaaggaaga ggcaaactcc ttcccctgga
      541 atgttcttga gataaaaaag ataggggtga gagatgtaag gaaggagagg caggttaagg
      601 ttccagcatc tctacttact caggatattg atagtattat taattctcca catatagatg
      661 taatagttga ggtagcaggt ggaagagacg aagggtttaa gattatcagc agggctataa
      721 atgcagggaa acatgttgtt actgccaaca aagatgttat agcctcttac ggcttagaac
      781 tatttagatt agccagaagt aaaggggtga agatctactt tgaggcaagt gttggagctg
      841 ggataccaat aataagagtc ttgagagaag atctggtagc caatagtatc caggaaatta
      901 gagcgatact gaatggtaca agtaattata tcctaacgag aatgtctgag aatggagagg
      961 actttggaac agctctgggt atggctcaga gattgggata tgctgagcca aatccagaca
     1021 atgatgtaaa aggggtagat gcagcctata agataaccat tctatcctcc atcgccttta
     1081 atacctctgt ctctcctgac gatatctatg tagagggtat agatactgta agtatcagag
     1141 atatagagta tgcccatgaa cttggttatg agataaaatc tcttgccata gccaggaaac
     1201 ataatgatgg tttagaatta agggttcatc ccactatgat ccccaggacg tctatgctgg
     1261 cagcggtgag gggtgtttat aatggtatat atatagttgg tgacgcagta ggaccgatga
     1321 tgttttatgg tagaggggca ggaagtttgc ccactgccag tgcagtggtt tctgatctct
     1381 gtgacctggt gaggtctctg cgggacggct caggtggaca gctcgaagta ccagcgctgg
     1441 acaatattcc gataagaccc atagagaaac ttgagacaag gtactatata agattgaatg
     1501 ttcctgatag gccaggggtc ctggcaggta tagctatggt atttggaaat ctgggtataa
     1561 gtatagcgag tgttgttcag aaggaggtcc cggatacaaa aggacaggct gagcttgtta
     1621 tcatcactca taccgctcag gaaggtccat tccgtcaggc tatatctcag atggataggc
     1681 ttgatgttgt gaataaggtt gaatccaccc tgaggataga aggagttgta gaagaatgag
     1741 atggaatggg gttcttagag aatacaaaga ctatcttcca gtaaccgata aaactccact
     1801 gataacgctc tatgaaggca atacccctct tatttatgcc aggaatatct ctaaggagct
     1861 tggacttaag gtttatctga agtatgaggg aatgaaccct accggttcat ttaaagatag
     1921 agggatggtg gtggctatag ctaaggctgt agaggagggt gccagggcag ttatatgtgc
     1981 ctctactgga aatacaagcg catctgcttc tgcctactct gctatagccg gcctgaagtc
     2041 tgtagtcctt atacccgaag gtgctatagc cctgggaaaa cttgcccagg ctcttactta
     2101 tggggctcag gttatagcgg taaaaggaaa t
//
LOCUS       DAOTKU010000301         2125 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_41881_length_2125_cov_3.588406, whole genome shotgun sequence.
ACCESSION   DAOTKU010000301 DAOTKU010000000
VERSION     DAOTKU010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41881_length_2125_cov_3.588406"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="P5310_06920"
     CDS             <1..411
                     /locus_tag="P5310_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU33129.1"
                     /translation="KTLALFLPSLILGWLLTFGSIVGINQIYVVPSSGQFIMPGPAPL
                     VAILVVIPIIFFFLCLLIIDMQLIITNIRWVNTALMVAVFTVSFGLSPVLKFGPSSWN
                     IVFVSLGVAAVLALISFLVSPRVTREKIVLSSKG"
     gene            429..671
                     /locus_tag="P5310_06925"
     CDS             429..671
                     /locus_tag="P5310_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU33130.1"
                     /translation="MFIDFFTNSIEANKLLLAERGVPISSKIQKSLLPFLGSSQREMF
                     NFIRLAEKNSVPTPPPDPAGANDVIKNIWNPIVEQI"
     gene            1191..1904
                     /locus_tag="P5310_06930"
     CDS             1191..1904
                     /locus_tag="P5310_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU33131.1"
                     /translation="MGITERRIEFMSTLDELCRENNGPVHYSLVAERLGVSKWTAYDL
                     LTALRDEGYVETLYILNDKGSGRSTVAFKLTDKGKDLIYKDTKEENVTKLSEEMRTRL
                     KSFEGLSVNELLQSGLKEISSSKTPVLKALNISIVIFMLSQKLAVNWDNISSSYQILS
                     KSIDPQSGLLGLTALLIGVILAKAGINKYGNTVNSKIGFILNKYKEILDELSHKDKEF
                     LFDITKEIYTGNILLGEEE"
     gene            1909..>2125
                     /locus_tag="P5310_06935"
     CDS             1909..>2125
                     /locus_tag="P5310_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRU33132.1"
                     /translation="MAFFDFAKKYVGGVVLEEGLNYLAKNPEQNIEKLINLARKFVKL
                     DYHKEQLEQVAKYLLDDTPQRELAVRLL"
BASE COUNT          635 a          360 c          448 g          682 t
ORIGIN      
        1 aagactttag ctctattttt acccagtctg atcttgggtt ggctgttaac ctttggcagt
       61 atagtgggca taaatcaaat ctatgtagtt ccctcgtcag gacaatttat catgcctggt
      121 cctgctccat tggtggcaat cctggtggtc ataccaatta tcttcttttt cttatgtcta
      181 ttaataatag atatgcaact cataattacc aatatacgtt gggtcaacac tgctctcatg
      241 gttgccgtat ttacagttag ttttggactt tctcctgttc taaagtttgg accctcatcc
      301 tggaacatag tatttgtttc actgggagtt gctgccgtct tggctctaat atctttcctg
      361 gtctcccctc gagttactag ggagaagatt gtcttgagca gcaaaggata gggttcatta
      421 aggttgctat gtttatagac ttctttacaa attccataga agcaaataag cttctacttg
      481 cagaaagagg tgttcctata tcttcaaaga tccagaagag cttacttcca ttcttgggtt
      541 catctcaaag agagatgttt aactttataa gattagcaga aaagaatagt gttcctactc
      601 caccgccaga tccagctgga gctaatgacg ttataaagaa tatatggaat cctatagttg
      661 aacagatata aaaagacccg tggaataaat tgttgcccgg gtctttttag tctttttata
      721 taaagtatta ttagatgcga gttgtgaacc tcattaaggg tgatttgtcg tcagggtgga
      781 ataagaagag aagagttcct gaaaacctat gagaaagcgt gagtaacttc gtctaacaga
      841 gcgtatctgg cttcggtagt ttgtatcttc gcctaaatcc tacttcgcac ttcacatctg
      901 cggctcttca actttttgcg tggggtaaat ctgttatata tatcctattt accctctgtc
      961 aatagtctgt gaaaatgtca gggaaaggaa atgaaacata tgagctgtat cccgtagggg
     1021 caattcatga attgccccta caatttcgtc ttttataata atctcgcacc tctattcctt
     1081 atgcgtcacc cattactcat gacaatatca cagaacgata acaccctctt gactttttcc
     1141 ttttattaat tataatttat atagagtatt ttgttttttt tggagggcat atggggatta
     1201 cagagagaag aattgagttt atgagcacat tggatgagct ttgcagggag aataatggtc
     1261 cagtccatta ttctctggta gcggagagac tgggagtaag taagtggaca gcatatgatc
     1321 tgcttactgc tctcagagat gaagggtatg tggaaaccct ttatatactg aatgataaag
     1381 gaagtggaag aagcacagtg gcttttaaac tcaccgataa gggtaaggat ttgatctata
     1441 aagatacaaa ggaggaaaac gttactaaac tttctgaaga gatgaggact cgtttaaagt
     1501 ccttcgaggg tctatctgta aatgagttgc ttcagtctgg gttaaaggag atatcctcat
     1561 ctaaaactcc agtactgaaa gcgctcaata taagtattgt tatatttatg ttaagtcaga
     1621 agttagcagt aaattgggat aacatatcct ccagttatca gattctttct aagagtatag
     1681 accctcagag tggactcttg ggtttaaccg ctctacttat aggtgttatt ctggctaagg
     1741 ctgggattaa taaatatggt aatactgtaa acagtaagat tggttttata ctgaataaat
     1801 acaaggagat actggatgag ctttctcata aggataagga atttttattt gatattacca
     1861 aagagattta tactggaaat attttattag gagaggagga atagacctat ggcattcttt
     1921 gattttgcta agaaatatgt tggtggggtg gtcttggagg aaggattaaa ttatcttgcc
     1981 aaaaacccgg aacaaaatat agaaaaactg attaatttag ccagaaaatt cgttaagctt
     2041 gactaccata aggaacaact cgagcaggtg gctaaatacc tgctggatga tacgcctcaa
     2101 agagaattag ctgtcagatt actga
//
LOCUS       DAOTKU010000302         2118 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_42072_length_2118_cov_2.799321, whole genome shotgun sequence.
ACCESSION   DAOTKU010000302 DAOTKU010000000
VERSION     DAOTKU010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42072_length_2118_cov_2.799321"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..841
                     /locus_tag="P5310_06940"
     CDS             <1..841
                     /locus_tag="P5310_06940"
                     /inference="COORDINATES: protein motif:HMM:NF019142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HRU33133.1"
                     /translation="CRVRFLLKEVMEISQKYIRMAVLYAFLLSFGAVVIWGELVFLKN
                     RFEEIDQKKELLRENEIVYISEKAKKEVSRTDVSVKYATLNFKSANKYSLLLPRWSIV
                     KEKKSVPFSVYRKYTTSLPYGATKTEPGKPGEVEIYWRVYKRGNEVLEKQKFKEVVLS
                     EPRHQIVYVGRGYMLASRGNFAGKPYLDMIATAYDPGVRSCGRYADGYTSLGLKAGYG
                     VVAVDPSVIPLRKKLYVEGYGYAIAGDVGSAIKGLKIDLGFDTYREAINFGVRRVRVY
                     ILD"
     gene            807..1619
                     /gene="rsmA"
                     /locus_tag="P5310_06945"
     CDS             807..1619
                     /gene="rsmA"
                     /locus_tag="P5310_06945"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HRU33134.1"
                     /translation="MLEGSGYIYSTKPKKSLGQHYLKDEKILRKIIRSLGLTGNEIVL
                     EIGAGHGELTYYLAQQSRELYAVEIDPSSVVFLREKLEPFHNVNIINGDILNFQSDIF
                     FDLIVGNIPYYISGLLLGTLSERWQYKKAVFTLQKEVGERLSALPGAKDYGVLTLAVC
                     YNHKVRVLFDVPRECFYPEPKVDSAVVELERIDDREIDKHFYMKVVRAAFSSRRKMLP
                     NVLINLGLDKEYIVKILRNLDINPERRGETLSVEEFVLLSERIYEALISGET"
     gene            1591..>2118
                     /gene="ispE"
                     /locus_tag="P5310_06950"
     CDS             1591..>2118
                     /gene="ispE"
                     /locus_tag="P5310_06950"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HRU33135.1"
                     /translation="MKLLSLAKLNLYLEILGKDSDGYHNIESIMQTISLSDTVIVEPS
                     STPGISISANLSSLPTDERNLAYKAAKLFMERVGWVSKGVKIILEKNIPLASGMGGGS
                     SNAATVLTAMALISGIDLLNSILVEIASSIGCDVPFFLYGGSAMIRGKGTDVTPLPPI
                     EGIPVLVVVPDVEVST"
BASE COUNT          649 a          312 c          515 g          642 t
ORIGIN      
        1 ctgccgggtc cgctttttat tgaaggaggt gatggagatt tcgcagaaat atataaggat
       61 ggcagtcctt tacgcctttc ttttaagttt tggcgctgtt gttatttggg gagagctggt
      121 atttttgaaa aatcggttcg aagagataga tcaaaaaaag gaacttctta gagaaaatga
      181 gattgtttat atatctgaaa aagcgaagaa agaagtatct aggactgatg tttctgtaaa
      241 atatgctacc ttaaatttca aatctgcgaa taagtattcg cttttattgc cacgttggag
      301 tatagtcaaa gagaaaaagt ctgttccttt ctctgtatat agaaaatata ctacatctct
      361 accatatggg gcaaccaaga cagaacccgg taaacctggt gaagttgaga tatactggag
      421 ggtgtataag aggggcaatg aggtgctgga aaagcaaaaa tttaaagaag tcgttctttc
      481 agaaccgagg catcagattg tttatgtagg tagaggatat atgcttgctt ccagaggtaa
      541 ttttgccggt aaaccctatc ttgatatgat agctactgct tatgaccctg gagttagaag
      601 ttgtggtaga tatgcagatg gttatacttc tctggggtta aaggcagggt atggggtagt
      661 tgctgtcgac ccttctgtca tcccactgag gaagaagctc tacgtagaag gttatggtta
      721 tgcaatagcg ggagatgttg gaagtgctat caagggacta aagatagatc ttggctttga
      781 tacatacaga gaggcaataa attttggtgt tagaagggtc agggtatata tactcgacta
      841 aacctaagaa atctttagga cagcactact taaaagatga aaaaattctg agaaaaataa
      901 taagatctct aggattaaca ggcaatgaga ttgtcctgga gataggggcg ggacatggtg
      961 aacttacata ctatttagcc cagcagagta gagaactcta tgcggtagaa atagaccctt
     1021 ccagtgttgt tttcctgaga gaaaaattag agccttttca caatgttaat attatcaatg
     1081 gtgatatatt gaactttcaa agtgatattt ttttcgacct tatagttggg aacatcccat
     1141 attacatatc tggattattg ttagggacat tgagtgagag atggcaatat aagaaggctg
     1201 tatttacgct gcaaaaagag gttggggaaa ggctgtcagc tttacctggg gctaaagact
     1261 atggtgtatt gactttagca gtatgttata atcataaagt gagggtttta tttgacgtac
     1321 ccagagagtg tttttatcct gaacctaaag tggatagtgc agttgtagag ctagagagga
     1381 tagatgatag agaaattgac aagcatttct atatgaaggt tgttagagct gcttttagca
     1441 gcaggagaaa gatgttacca aatgtgctga ttaatttggg tttggataaa gagtatatag
     1501 ttaaaatact aagaaattta gatattaacc ctgagagaag aggggaaact ttgtcagtag
     1561 aagaatttgt tttattatcg gagaggatat atgaagctct tatctctggc gaaacttaat
     1621 ttatatctgg agatacttgg taaagattca gatggttatc ataatataga aagtataatg
     1681 cagactatat ctctgtctga tactgttata gtagaaccat ccagtactcc tggtatatct
     1741 atctctgcta acctctcatc cttacccaca gatgaaagaa atctggctta taaggcggct
     1801 aaactcttta tggaaagggt tggctgggtg tctaagggtg taaagattat actcgaaaag
     1861 aatatccctc ttgccagtgg aatgggggga ggtagcagta atgctgctac tgttttaaca
     1921 gctatggctc ttatatccgg aatagacctg ttgaattcta tccttgtaga gatagcatcg
     1981 agtattggct gtgatgtccc attctttctc tatggcgggt ccgccatgat aagggggaag
     2041 ggaacagatg ttacaccatt acctccaatt gagggaatac ctgtcctggt tgttgtcccg
     2101 gatgtggagg tttctacc
//
LOCUS       DAOTKU010000303         2105 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_42444_length_2105_cov_2.171707, whole genome shotgun sequence.
ACCESSION   DAOTKU010000303 DAOTKU010000000
VERSION     DAOTKU010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42444_length_2105_cov_2.171707"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            230..305
                     /locus_tag="P5310_06955"
     tRNA            230..305
                     /locus_tag="P5310_06955"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:263..265,aa:Met,seq:cat)
     gene            273..1688
                     /gene="tilS"
                     /locus_tag="P5310_06960"
     CDS             273..1688
                     /gene="tilS"
                     /locus_tag="P5310_06960"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HRU33136.1"
                     /translation="MSEVRVLVPLPLSMNFTEKVKEFILKYNLIFPEDTVLIGVSGGP
                     DSMALLYTLFSLMNELNFDIAVCYVDHKLRPESKDEAKFVSEVAESLKLPFTLKEINI
                     EKFRRGRTLEESARVARYRALEEARKENNGTRIAVAHHLDDQIETILMRIVRWTSIRG
                     LRGIPVRNGNIIRPLLGVYREEILGFCGENNIRYVIDSSNLSTAFTRNRIRLELIPVL
                     EQYNPKVKESLLRLSAQAEELDGFLSYLEDDLLKRYKISGDEYYIAADSIKSLPEFLK
                     RRVIFRILVEVSPKPTDVREESYKMLTSLLDKSTGKILNLPGGAIAYRDYNGIRILRE
                     NPHLLPVYILNIPGVTVIDRLKKKITTEIISKKELFEMASPNQYTFYLDSDKIELPIY
                     LRGPKEGERFSPLGLNGKMKKLQDIMVDRKIPREYRGIYPVIVDNRGEVICIPEYTIS
                     ERVRVDEGTKNILLLRIECYE"
     gene            1681..>2105
                     /gene="hpt"
                     /locus_tag="P5310_06965"
     CDS             1681..>2105
                     /gene="hpt"
                     /locus_tag="P5310_06965"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HRU33137.1"
                     /translation="MNNNIEVLFTEEEIRDRVKELGQEITRTYVGKKPHLVCVLRGAF
                     IFLSDLVRYIDLPVTVDFMAVSSYSKGTKSTGIVKIIKDLDESIENRDVIIVEDIIDT
                     GLTLEHLVKLFWARNPKDVKICVLLDKEARRITRIGLDFV"
BASE COUNT          669 a          362 c          513 g          561 t
ORIGIN      
        1 ggtcagcgaa gtgtggggag cgggggacgc cggcttcgca gcggaggggg tcgcgtgacg
       61 ctggtggcca ccgagaccgt ggcgacgatg gaacgcgaga agtcggctat ctgctgtctg
      121 gcgctgcatt caggactcct tgtcctgaag cgcgcaacac cctgatgctc gtcgggctca
      181 taacccgaag gtcgctggtt caaatccagc ccccgctacc attttttatg gcggcatagc
      241 tcaggtggta gagcaggcgg ctcatacccg ccatgtccga ggttcgagtc ctcgtgccgc
      301 taccactttc gatgaatttt acagaaaaag taaaagagtt tattctaaag tataatctca
      361 tctttcctga agatacagtc ctaattggtg tatctggggg tcctgattct atggctcttc
      421 tttataccct cttctctctt atgaacgagc tgaatttcga tatagctgtc tgttatgttg
      481 accacaaact ccgacctgag agtaaagatg aggcaaaatt tgtctcagaa gttgcagaaa
      541 gcctgaagtt accctttact ctgaaagaga ttaatataga gaagtttaga agaggcagga
      601 ctttagaaga atcagccagg gttgcaagat acagggcact ggaggaagcc agaaaagaaa
      661 ataatggaac caggatagcg gttgcccatc atctggatga ccagatagag accattctta
      721 tgcgtattgt caggtggaca agtatcagag gactcagagg tattccagta aggaatggga
      781 atattatcag acctctcttg ggagtatata gagaagagat attaggattt tgtggggaga
      841 ataatataag atatgttatc gatagctcaa acttaagtac tgcttttaca agaaacagga
      901 taagattgga gctcatacct gtactggaac agtacaatcc aaaagtaaaa gaatcacttt
      961 taagattatc cgctcaggca gaagagctgg acggatttct ctcctactta gaagatgacc
     1021 tattaaaaag atataagata tctggtgacg agtactatat agctgcagat tctataaaaa
     1081 gcctgccaga gtttcttaag agaagggtaa tctttagaat actggtggaa gtaagtccta
     1141 agcctactga tgtaagagaa gaatcttata aaatgctaac ctcactgcta gataaatcta
     1201 caggtaagat cttaaacctc cccggtggag ctatagctta cagagactat aatgggatca
     1261 ggattctcag agagaaccca catttactgc ccgtatacat attgaatata cctggagtta
     1321 ctgtgataga tagacttaag aaaaagataa ccactgaaat tatatcaaag aaggaacttt
     1381 ttgagatggc atcgccaaat cagtatactt tttacctgga ttctgataaa atagaattac
     1441 cgatttattt aagaggtcct aaagagggtg agagattttc tcctcttgga ctcaatggta
     1501 aaatgaagaa attacaggat attatggttg acaggaagat accaagagag tataggggta
     1561 tataccctgt tattgtggat aataggggtg aagttatttg tatcccggag tatactatta
     1621 gcgaaagagt aagggtggat gaaggtacca aaaacatatt attattgagg attgaatgtt
     1681 atgaataaca atatagaagt cctgtttacg gaagaagaga taagagacag ggtaaaggaa
     1741 ctgggacagg agattaccag gacttatgtg gggaaaaaac cacatcttgt ctgtgttctc
     1801 cggggagcgt ttatattcct atctgacctg gtaaggtata tagaccttcc tgttacagta
     1861 gattttatgg ctgtatccag ttatagtaag ggaacaaaat ctactggaat agttaaaata
     1921 ataaaagacc tggatgagtc tatagagaat agagatgtga ttattgtaga agatataata
     1981 gacacagggc ttactcttga gcatctggtc aagctcttct gggccagaaa cccgaaagat
     2041 gttaaaattt gtgttttact tgataaagag gcaaggagga taacaaggat agggctggac
     2101 tttgt
//
LOCUS       DAOTKU010000304         2104 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_42465_length_2104_cov_2.823328, whole genome shotgun sequence.
ACCESSION   DAOTKU010000304 DAOTKU010000000
VERSION     DAOTKU010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42465_length_2104_cov_2.823328"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /locus_tag="P5310_06970"
     CDS             <1..798
                     /locus_tag="P5310_06970"
                     /inference="COORDINATES: protein motif:HMM:NF015922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HRU33138.1"
                     /translation="KELDKILFFSDRGLVYQLKGYQIPISSRQARGTSISLILPVKDE
                     EKITAIFPVPEDLDTFGKIVMVTSKGTIKLANLKEYVDPPNRGKIAISLQDGDSLVGV
                     EALMGDEDLILVSNKGQLARFSVDSLREMGRTARGVRGMRLDPDDFVIDVETFEPGKY
                     LLFITKAGYGVKIALDDIPQKKSRGIKGVKGLKFRKGDGLADILKVGEDDEIVIVTAL
                     GKTIRIAVKGIRQVGRNNGVGMKIMSLDDGDYVQSAALVENELEDEI"
     gene            788..1204
                     /locus_tag="P5310_06975"
     CDS             788..1204
                     /locus_tag="P5310_06975"
                     /inference="COORDINATES: protein motif:HMM:NF001617.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HRU33139.1"
                     /translation="MKFELVEHTADIGLIAYGKTVEELFENASEGLFSILTDITKVRP
                     LDEYKLAVSGEDLEDLFIRWLNELIYFWEVKRVLASRFSVNIDNNSLEGLIYGESFDH
                     NRHTIEHGVKAVTYYDFRIEYDKLNEIWKAKVIIDV"
     gene            1219..>2104
                     /locus_tag="P5310_06980"
     CDS             1219..>2104
                     /locus_tag="P5310_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013431124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRU33140.1"
                     /translation="MYMADSNRYSEMLYRRCGKSGLKLPAISLGLWHNFGYGDSFDNM
                     RSIVTRAFDLGVTHFDLANNYGPPPGSAEENFGRILKFDLKSYRDELIISTKAGYRMW
                     DGPYGEWGSKKYLIASLDQSLKRMNLDYVDIFYSHRPDPETPIEETMEALYQAIRQGK
                     ALYVGISNYNSEQTRAAYDALRSMGVRLIVNQVRYNMFARDIEKTLLGTLDELGMGAV
                     IYSPLAQGLLTERYLEGIPEDSRVRKSGIFLRESDITPERIEKVRRLTEIAKLRGQTM
                     SQLALSWILRHNTVATVIV"
BASE COUNT          657 a          326 c          551 g          570 t
ORIGIN      
        1 aaagaactcg ataagatact cttcttctct gatagagggc tggtatacca gttgaaaggt
       61 tatcagattc caatttccag cagacaggca agggggacat ccatctctct catcctccct
      121 gtaaaggatg aagaaaaaat aacggcaatc ttccctgtcc cggaggatct cgatacgttt
      181 ggtaagatag ttatggttac aagtaagggg actattaaac tagcaaatct gaaagaatat
      241 gtcgatcctc caaacagggg aaagatagcc atatcacttc aggatggaga tagcctcgtt
      301 ggggtagagg ctctgatggg tgatgaagac ctcatactgg tgagcaataa gggacaactt
      361 gccagatttt ccgttgattc cctcagggag atggggagga ctgcaagagg ggttcgtggt
      421 atgaggcttg accctgatga ttttgtcata gatgtggaga cctttgagcc tggaaaatat
      481 ttactcttta ttacaaaggc aggttatggg gtaaagatag ctctggatga tatacctcaa
      541 aagaagagca gagggataaa gggggtaaaa ggactcaaat tcagaaaagg ggatgggctg
      601 gctgatatac ttaaggtggg ggaagatgat gagatagtaa tagtgaccgc tttaggaaag
      661 accatcagaa ttgcagtaaa aggtataagg caggttggta gaaataatgg tgtggggatg
      721 aagataatgt ctctggacga tggtgattat gtgcagagtg cagccctggt tgagaatgaa
      781 ttagaggatg aaatttgaac tggtagaaca tacagctgat ataggtctga tcgcatacgg
      841 taaaaccgta gaagagcttt ttgagaatgc tagtgagggt ttattctcta tactcaccga
      901 tattacaaaa gtaagaccgt tggatgagta taaattagct gtctccggag aagacctgga
      961 agatttattt atccgctggc ttaatgaact catatacttc tgggaggtaa aaagagtact
     1021 ggcttcaaga tttagtgtaa acatagacaa taactctctg gaaggactga tttatggaga
     1081 gagttttgac cataacaggc atactataga gcatggagtg aaggcagtaa cttattatga
     1141 tttcaggata gagtatgata agctcaatga aatttggaaa gccaaagtga taatagatgt
     1201 atgaggaggt gctgaaacat gtatatggca gattcaaata gatattcaga gatgctttat
     1261 agaagatgtg gaaagagcgg gttgaaactt cctgctattt ctcttggact ctggcataat
     1321 tttgggtacg gagattcttt tgataatatg cgttcaattg taacaagggc ttttgacctg
     1381 ggagttaccc attttgacct tgccaataac tatggtcctc cccctggttc tgctgaggag
     1441 aattttggaa ggatattgaa gtttgacctg aagtcatata gagacgagct gataatttcg
     1501 accaaggcag ggtatagaat gtgggatgga ccttatggag aatggggttc aaagaaatat
     1561 cttatagcaa gtcttgatca gagtttaaaa aggatgaatc ttgattatgt tgacatattc
     1621 tactctcacc gacctgatcc tgagacgccg atagaagaaa ctatggaggc attatatcag
     1681 gctattcgtc aggggaaagc cctgtatgtt ggcatatcaa attacaactc agaacaaaca
     1741 agagctgctt atgatgctct cagatctatg ggggtaaggc ttatagttaa tcaggtgaga
     1801 tataatatgt ttgccagaga tatagaaaaa acccttctcg gtactctgga tgaattggga
     1861 atgggtgctg ttatctactc cccgctggct caggggttac ttacagaaag atatctggaa
     1921 ggtatcccag aagattccag ggtaagaaag tcagggatat ttttgaggga gagtgacatt
     1981 acacctgaga ggattgaaaa ggtaaggaga ctgacagaga tagcgaaatt acgtggtcag
     2041 actatgtctc agctcgcact gtcctggata ttgagacata atacagttgc cactgtgatt
     2101 gttg
//
LOCUS       DAOTKU010000305         2101 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_42546_length_2101_cov_3.768817, whole genome shotgun sequence.
ACCESSION   DAOTKU010000305 DAOTKU010000000
VERSION     DAOTKU010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42546_length_2101_cov_3.768817"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="P5310_06985"
     CDS             complement(<1..278)
                     /locus_tag="P5310_06985"
                     /inference="COORDINATES: protein motif:HMM:NF012965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HRU33141.1"
                     /translation="MTRIIDGKSIAEDIYKELKEEVDRLGDKPGLAVVLVGDDPASLV
                     YVRNKERACKRLNLSYRLVRFPSSVSREELKSSIEELNMDKSINGIIV"
     gene            complement(294..1289)
                     /locus_tag="P5310_06990"
     CDS             complement(294..1289)
                     /locus_tag="P5310_06990"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRU33142.1"
                     /translation="MKRYYISQLKEGIQLDGVPFVVLKKSLGMSKNGKSSLTLTLGDK
                     TGSILARRWDYEGGEVSTGTVYEVSGRVIIYNNNIEIALDRLTPASHFDPRDFIPQSP
                     RDSLALLGELKKKTDEVQDPGIKELLKSFLYDEKFVNKFLLAPAGKVIHHAYIGGLIE
                     HTWEVCRISMVAGEMYPEINKDLLIAGSILHDIGKVEELTYSTQIEYSTKGRLLGHIF
                     IGAIVVNERARNINNFNPSYLQQLSHIILSHHGDYSTGSPVLPMTLEAETVHRADYLS
                     AQVNRFYNILKDKPEERWIKGDNLLGRQLYKSKVKNLTVEEILMSEIERLEEEEQ"
     gene            complement(1286..2077)
                     /locus_tag="P5310_06995"
     CDS             complement(1286..2077)
                     /locus_tag="P5310_06995"
                     /inference="COORDINATES: protein motif:HMM:NF013292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase family
                     protein"
                     /protein_id="HRU33143.1"
                     /translation="MKPVKLIVHGGAGDISSDTMEIYQKNLKEIWQSGYNLLKEGWDS
                     LSVVEKVISLIEDTGVFDAGTGSFPNKEGYIEMDASIMTSDGRAGAVGGVRTIRNPIR
                     LARLVMEETSHVMLVGEGAERFAEQFNLPPLTPRESPYSISTDTVGAVAMDKRGLISA
                     GTSTGGIRGKIPGRIGDSPIIGAGTYASFWGGISSTGFGEDIIKFGLAKKIDILLRQF
                     PAQTAVDMVVEESKYRGIEFGVIVLDRLGNTGVGYTAKAMAWYPR"
BASE COUNT          523 a          538 c          332 g          708 t
ORIGIN      
        1 tggacaatga taccatttat ggacttatcc atattaagtt cttctatgct cgattttagc
       61 tcttctctgg agacactgga tggaaatcta accagcctat aacttaaatt taatctcttg
      121 catgcccttt ccttattcct cacgtagacg agagaggcag ggtcgtcccc tacaagtacc
      181 actgccagtc caggcttatc accgagcctg tcaacctctt ctttgagttc tttatagata
      241 tcttctgcta tacttttccc atctattatc ctagtcatct ctattcctcc aaactactgt
      301 tcttcctctt caagtctttc tatctctgac attaatatct cttctaccgt taagttctta
      361 actttactct tatataattg tctccctaac agattatctc cctttatcca tcgttcttca
      421 ggtttatctt tcagaatatt gtaaaacctg tttacctgag cactcagata atctgctctg
      481 tgcacagtct cagcctcaag ggtcattggt aatacaggag aaccggtaga gtagtctcca
      541 tgatgactga gtattatatg tgacagctgt tgcaggtaag aagggttaaa gttatttata
      601 tttctagccc tttcattgac cactatagcc cctataaata tatgtcccag taatctacct
      661 ttagtggagt attctatctg agtgctgtat gttaattcct ccacctttcc tatatcgtgt
      721 aatatggaac cggctattaa caggtctttg tttatctcag gatacatctc ccctgctacc
      781 atggagatcc tgcatacctc ccaggtatgt tctatcaatc cacctatata cgcatgatga
      841 attacctttc ctgctggagc aaggagaaat ttattaacaa atttttcgtc gtataagaat
      901 gatttgagta gctcttttat acctggatcc tgcacctcat cggtcttttt ctttaattca
      961 cccaacaagg cgagtgaatc tcttggactt tgtgggataa aatctctagg gtcaaaatga
     1021 ctggctggag tcagtctatc cagtgctatc tcgatattat tgttgtatat tatgaccctt
     1081 ccactaacct cataaactgt cccggtagat acctctccac cctcataatc ccatcttctg
     1141 gccagtatag aacctgtttt atctccaagc gtaagggtaa gagagctttt cccattctta
     1201 ctcatcccca atgatttctt caggacaaca aatggaaccc catccagttg aattccttct
     1261 tttaactgtg agatatagta tctcttcatc ttggatacca tgccattgcc tttgcagtat
     1321 atcctacccc agtatttccc agtctatcca gcactattac cccgaattct atccctctat
     1381 attttgattc ttctaccacc atatccaccg ccgtttgagc tggaaattgt ctcagtaata
     1441 tgtctatctt tttggcaaga ccgaacttta ttatatcctc cccaaatccg gtggatgata
     1501 ttcctcccca gaacgaggca taggtacctg cccctatgat aggactatct cctatcctcc
     1561 ctggaatttt ccccctgatc ccaccggtag atgtcccggc agatatcagt cctcttttat
     1621 ccatagctac tgcccctacc gtatctgtgg atatgctgta aggagattct ctgggggtaa
     1681 gtggaggaag attgaattgt tcggcaaatc tttccgctcc ctctccaacc agcattacat
     1741 gggaggtttc ttccataact aatcttgcca ggcgtattgg atttcttatt gtccttaccc
     1801 ctccaacagc tccagcccgt ccatctgagg tcataatact tgcatccatc tctatataac
     1861 cttccttatt tggaaaggaa cccgtaccag catcaaaaac tccggtatct tctataagac
     1921 ttataacctt ttctacaact gataacgagt cccatccttc tttcagaaga ttataccctg
     1981 attgccatat ctcttttaga ttcttctgat atatctccat ggtgtcactc gagatatcac
     2041 cagctcctcc atgtactata agtttaacag gtttcatttg agataaacct ccttaatgcg
     2101 c
//
LOCUS       DAOTKU010000306         2083 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_43045_length_2083_cov_3.289448, whole genome shotgun sequence.
ACCESSION   DAOTKU010000306 DAOTKU010000000
VERSION     DAOTKU010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43045_length_2083_cov_3.289448"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1364
                     /locus_tag="P5310_07000"
     CDS             <1..1364
                     /locus_tag="P5310_07000"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HRU33144.1"
                     /translation="VKVGKIEEPAVMNISADQLAVSPGFIDIHSHSDLSSRNFPEMEN
                     ILRQGVTTQVVGNCGITPGILEGSLGRFIEDIEKRGIGTNLIFLAGQGSIRSKVMGRE
                     NRRPTEDELEKMKSILESAIDEGAKGLSTGLEYIPGIFSSTEEIAELAKVAARRGGIY
                     STHLRSEGDELELAVKEAIEIGRLADIPVQIAHLKTEKKRNWGKSERILQLIDSARVN
                     GTDVNCDVYPYTAYMTSLDFAVQPFDLSAYKNYREALEGLKKTPVDWDLIYISKCDMD
                     GGINGCNVLELARRKGMSPEELALELLNNDDPVRVISFQMSEEDVKNIIAYRHSFIGS
                     DGSGYRKRDEFDRTHPRSFGTFPRILGRYVRELKLLSLEEAISKMTSLPAKKLNISDR
                     GIVKEGAYGDITIFNPDKITDKATYDDPFQYPEGIEYVILNGKIIIDKGIFSDKRAGI
                     VIR"
     gene            1459..>2083
                     /locus_tag="P5310_07005"
     CDS             1459..>2083
                     /locus_tag="P5310_07005"
                     /inference="COORDINATES: protein motif:HMM:NF014273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HRU33145.1"
                     /translation="MKRVDNIRSLQEEYGGLGYLRVKDTGVHPIEVDKIIGSVGRPQD
                     LDSNFRFKWRRPTERYLTIKKMMEEGKILPPIEVYKIRDEYYVLDGHHRVAAAKELRQ
                     KFIDAHIFQAYPLRPRREDILYSYRESFREKTGLDIPLTEPSGYENFLSQIEDYAKRN
                     NVSFKEAVSGWYKEFLEDCRLIKREKVDRIFRDKTIGDLYLYVCTHKW"
BASE COUNT          683 a          303 c          526 g          571 t
ORIGIN      
        1 tagtgaaggt aggaaagata gaagagcctg ctgttatgaa tatttctgca gaccagttag
       61 cagtatctcc tggctttata gatattcatt cccactcaga cctctcttcc agaaattttc
      121 ctgagatgga gaatatcttg agacagggtg tgaccaccca ggtggttggg aattgcggta
      181 taactcctgg tatcctggag gggagtcttg gtagatttat agaggatata gagaaaagag
      241 ggataggaac aaatctgata ttcctggcag ggcagggatc gataaggtct aaggtgatgg
      301 gaagagagaa cagaagacca accgaagatg agctggagaa gatgaagtct atccttgaat
      361 ctgctataga tgaaggggct aaaggtctat ctacggggtt agagtatata cccggaatat
      421 tctcttccac tgaagagata gcagagctgg caaaagttgc agctagaaga ggtggtatat
      481 attcgactca tctgagaagt gagggtgatg agttagaact ggcggtgaaa gaggcgatag
      541 agataggtag acttgcagat atccctgttc agatagccca tttaaaaaca gaaaagaaga
      601 ggaactgggg taaatcagag agaattttac aattgataga ttcagccaga gtaaatggta
      661 ctgatgtgaa ctgtgatgtt tatccatata ctgcttatat gacaagttta gattttgcgg
      721 tacaaccttt tgacctgtct gcatataaaa actatagaga agccctggaa ggattaaaaa
      781 agacccctgt agactgggac ctgatatata tctctaagtg tgatatggat gggggtataa
      841 atgggtgtaa cgtgttggag ctggcgagga ggaaaggaat gtcacccgaa gaacttgcgc
      901 tggaattact gaataatgac gatccggtaa gggttataag ttttcagatg agtgaggaag
      961 atgtaaagaa tataatagct tacagacact cttttatagg ttcggatggt tctggttata
     1021 gaaagagaga tgaatttgat agaacccatc cccgttcttt tggcacattc cccagaattc
     1081 ttggaagata tgtcagggag cttaaacttt tgtctttaga agaggcaata tcaaagatga
     1141 ccagtctacc tgctaagaag ttaaatatct cagatagggg gattgttaaa gaaggtgctt
     1201 atggagatat tacaatattt aatcctgata agataacaga taaggctacc tatgatgacc
     1261 cattccagta tccagaaggt atagagtatg ttattctaaa tgggaaaatt attatagata
     1321 agggtatatt ttcagataag agggctggga tagtaataag atagttaacc agttcttaat
     1381 cttatattaa actgtcttta attctatctt aaatatcgtt taaacctctt cctgtaagat
     1441 ttaattgggg tgataaggat gaaaagagta gataatataa ggtcattaca ggaagagtat
     1501 ggtggtttag gttatctgag agtaaaagat acaggtgttc atcccataga ggtggataag
     1561 attataggtt ctgtgggaag accccaggac ctggattcca acttcaggtt taaatggaga
     1621 agacccactg aaaggtatct tacaataaaa aagatgatgg aggaggggaa gattttacct
     1681 cctatcgaag tttacaagat aagggatgaa tactatgtgc tggatggaca tcacagagtc
     1741 gctgccgcca aagaactgag acagaaattt atagatgccc atatctttca ggcttatccc
     1801 cttagaccca ggagagagga tattctgtat agctatagag agtcctttag agagaaaaca
     1861 ggattggata tacctcttac cgagccttcg ggttatgaga attttctctc tcagatagaa
     1921 gattatgcaa agaggaataa tgtctccttt aaagaggcag taagtggctg gtataaggaa
     1981 tttctggagg attgcagact gataaagaga gagaaggttg acaggatttt tagagataaa
     2041 acaattgggg acctgtacct ctatgtctgt acacataagt ggt
//
LOCUS       DAOTKU010000307         2070 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_43452_length_2070_cov_2.727047, whole genome shotgun sequence.
ACCESSION   DAOTKU010000307 DAOTKU010000000
VERSION     DAOTKU010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43452_length_2070_cov_2.727047"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..958
                     /locus_tag="P5310_07010"
     CDS             <1..958
                     /locus_tag="P5310_07010"
                     /inference="COORDINATES: protein motif:HMM:NF015377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRU33146.1"
                     /translation="FMAEGAEAASFSLVSVPYYNIEGYPLVSCISMVLGYFGTEVDQE
                     ELKTRLVFSGIENPFNAVDYMDSKGFKMYVTQLQIREIKNLIDRSEIPVIVGQSFRRP
                     YDYIYWRVVIGFDDEKGIITNDPILRNNYQIAEDKFNSLWVREAPNITIVIVPKDKKL
                     SITENNTVKNAMSMYFSAISYISKSDWKSARAELEKYLKIYPDNPLGLNAYAYVLLQL
                     GELENAKGAINKVLPKYPLPFIYDTAGLIYWKLNEIDRAEQYFYNAYISTPSNREIVK
                     NYANFLIAQAKIDEAKGILNSYLLIQPEDGEIKSLLDSLNSR"
     gene            974..1825
                     /locus_tag="P5310_07015"
     CDS             974..1825
                     /locus_tag="P5310_07015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HRU33147.1"
                     /translation="MLVTTDILFKEAVNSDYAIGAFNISNREFLVAIILAAKESNSPL
                     IIQVSESTVKFLGFDYISHLIEYADKFSENIPIVVHLDHSRRLETIVGAIRAGFTSIM
                     YDGSHLPFEENVESTREIVKISKAVGIPVEGEIGKIGGVEEHIKVKEDEVIYTSVEEA
                     VRFYELTGVTSLAVAIGTAHGMQKKNARINFQRLEEIHSKLPDVPLVLHGSSGVPKED
                     LKTAIRFGIKKVNIATELKMAMADGIKRILDGGAIEPRDYLNEGIENVKREVLNNIEI
                     LGSADRV"
     gene            1828..>2070
                     /locus_tag="P5310_07020"
     CDS             1828..>2070
                     /locus_tag="P5310_07020"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRU33148.1"
                     /translation="MGIDKNKKIVGVDLGGTTFTVGIMDFNGNILSSVDTDTPVENGP
                     SAVMGKILDNIDLAINEAKLSREDILGVGLGAPGILD"
BASE COUNT          672 a          299 c          494 g          605 t
ORIGIN      
        1 ttttatggct gaaggagcag aggctgcttc tttttcatta gtatctgttc cttactataa
       61 tatagaaggg tacccattag taagttgtat ctctatggtc ttgggttatt tcgggacaga
      121 agttgatcag gaagagctga agaccagatt ggttttttct ggtatagaga atccgtttaa
      181 cgctgtagat tatatggata gtaagggatt taaaatgtac gtaactcagc ttcagataag
      241 ggagattaaa aatcttatag accgttcaga gattcctgta atagtcggac agtcttttag
      301 aagaccgtac gactatatat attggagagt agttataggt tttgacgatg agaaggggat
      361 aataaccaat gaccccatcc tcaggaataa ttatcagata gctgaagata aatttaattc
      421 tctctgggtg agagaggctc cgaacattac catagttatt gtccccaagg ataaaaaatt
      481 gagtataact gaaaataata cggttaagaa tgcaatgagt atgtatttta gtgctataag
      541 ttatatatct aaatctgatt ggaaatctgc aagagctgaa ctggagaaat atctgaagat
      601 atacccggat aatcctcttg gtctgaatgc gtatgcctat gtgttgcttc aactgggaga
      661 attggagaat gctaaaggtg cgataaataa ggtcttacca aagtatcctc ttccatttat
      721 ttatgacacc gccgggctta tatactggaa actcaatgag atagacaggg cagaacaata
      781 tttctataac gcttatatat ccactccaag taacagagag atagtaaaga actatgctaa
      841 ttttcttata gcacaggcaa aaattgatga agctaagggc atcttgaata gttatctact
      901 tatacaacca gaagatggag agataaagag tttattggac agcttaaatt ctagatagaa
      961 aggagatagc aagttgttag tcacgacaga tatccttttt aaagaggcgg tcaacagtga
     1021 ttatgctatt ggggctttta acatctctaa cagagaattt ttagtagcta ttatccttgc
     1081 cgctaaagag tcaaattcac cccttataat ccaggtaagt gaaagtacag tgaaattcct
     1141 gggttttgat tatataagtc atcttattga atatgcagat aaattctcgg agaatatccc
     1201 catagtcgtt catctagatc attctcgtag attagaaaca atagttgggg ctataagggc
     1261 aggttttaca tcgataatgt atgatggttc acatcttcca tttgaagaaa atgtagagag
     1321 taccagagag atagtaaaga taagtaaagc ggtggggata ccggtggagg gggagatagg
     1381 gaagattgga ggagtggaag agcatataaa ggtaaaggag gatgaggtta tctatacctc
     1441 tgtggaggag gcggtaaggt tttacgagct taccggagtc acatctctgg ctgtggctat
     1501 tggtaccgct cacgggatgc agaagaagaa tgccagaata aacttccaga gattagagga
     1561 gattcattct aaacttcctg atgtaccttt ggtcctccat gggtcttccg gtgttcccaa
     1621 ggaagacctg aagactgcta tcagatttgg tataaaaaag gtaaatatcg ctaccgagtt
     1681 gaaaatggct atggcagatg gtataaagag aattttagat ggtggggcta tagagccaag
     1741 agattattta aatgaaggca ttgaaaacgt aaagagggaa gttttaaata atattgagat
     1801 tctgggaagt gcagacaggg tataaatatg ggtatcgata aaaataagaa gattgttggt
     1861 gtagatctgg gtggaaccac ttttacagtt ggcattatgg attttaatgg taatatcctc
     1921 tccagtgtgg atacagatac acctgtagag aatggaccat ctgctgttat gggtaaaatc
     1981 ctggataata tagatttagc tataaatgaa gctaagctta gcagagagga tatacttggg
     2041 gtaggtttgg gtgcccctgg tattctggat
//
LOCUS       DAOTKU010000308         2069 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_43468_length_2069_cov_4.615194, whole genome shotgun sequence.
ACCESSION   DAOTKU010000308 DAOTKU010000000
VERSION     DAOTKU010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43468_length_2069_cov_4.615194"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="P5310_07025"
     CDS             <1..622
                     /locus_tag="P5310_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRU33149.1"
                     /translation="LGVAALFFVRETKKLKSSDASKPVLKWSLLDRRLKGFLIVTLIF
                     TLGNSSNQFLLLRAKDMGFNDTTVLLLYLAYNIVYMIFAYPAGRLSDRIGRKRLIVTG
                     YLFYGLVYLGFAIVGNASYLWLLFGLYGIYIGLTEGVQKALISDIAPVETRATLIGLH
                     ATLEGIGLLPASFIAGLLWNTVSASAAFYFGGAMGIIAAVGMLLVL"
     gene            complement(625..1908)
                     /locus_tag="P5310_07030"
     CDS             complement(625..1908)
                     /locus_tag="P5310_07030"
                     /inference="COORDINATES: protein motif:HMM:NF021645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoPQ-activated protein PqaA family protein"
                     /protein_id="HRU33150.1"
                     /translation="MKKMVKILVLTSLFIFLISNAYGQTLIDYVGKDDGCFSWEIISR
                     EDVEGATIFNINLVSQRWKDIVWSHRLVIGVPKTIINPKTGVLFITGSWGGYDSEEVL
                     YIKEMTRNLGCVAAVLFDVPNQPLFGGLREDALIAYTLARYVETGDEEWPILLPMAKS
                     AVRAMTATQEFSKRELNIDIEGFLVTGASKRGWTTWLTGACDGRVKGIAPMVYDNLNI
                     KAQFSHQREIWQDLSLKLYDYTSLDLDKLADSPIGEKVLSIIDPYYYRERLTVPKLII
                     NGSNDPYWSIDAINLYFKDLPGEKYVLYVPNSGHNLEDRERVLKDVFAFYLYTAGRIP
                     FPKIGYETVEDDTSINLILKSDIKPAKVGLWYAESDNKNFVHSVWKYIEAEESEDKYT
                     VRLERPEDKSLAFFGEFYYNIGGVELYLCTPASLR"
BASE COUNT          577 a          455 c          395 g          642 t
ORIGIN      
        1 tcttggcgta gctgctctct tttttgtaag ggagactaaa aagcttaaga gttcggatgc
       61 ttctaaacct gtattgaaat ggtcattgct ggatagaaga cttaaggggt ttctgatagt
      121 taccctcatc ttcacacttg gcaactcttc caaccagttc ttacttctca gggcaaagga
      181 tatgggattt aatgatacga ctgtcctgct actctatctt gcctataaca tcgtatatat
      241 gatatttgcc tatcctgccg gaaggctatc agacaggata gggaggaaaa gacttattgt
      301 aacaggatat ctcttttatg gacttgtata tctgggattt gctatcgtgg gcaatgcatc
      361 ctacctctgg ttactctttg gtctttacgg tatctatata ggtcttaccg aaggcgtaca
      421 gaaagccctt atatccgata tagcccctgt agaaacaagg gcaactttaa taggtcttca
      481 tgcaacacta gaaggtatag gactattgcc agcatcattc attgcagggc tcttatggaa
      541 tacagtctca gccagtgccg cattctattt tgggggagcg atgggtataa tagctgcagt
      601 agggatgctg ctggtcctgt aggattatct caggctggct ggagtacaga gataaagctc
      661 cactccacct atattatagt agaactcccc aaaaaaggct aagctcttat cttcaggtct
      721 ttccagcctg acagtatatt tatcttcgga ttcttctgcc tctatatatt tccatacaga
      781 atggacaaag tttttattat ccgattctgc ataccaaagt ccaaccttcg ccggctttat
      841 atccgattta agaataagat ttatggatgt atcgtcctca actgtttcat accctatctt
      901 cgggaatgga attcttccag ccgtatagag gtagaaggca aatacatcct ttaaaactct
      961 ttctctatct tccagattat gtccagaatt tggaacatat aatacatact tctcacctgg
     1021 aaggtcttta aagtagagat ttatagcatc tatagaccag tatgggtcat ttgaaccgtt
     1081 aattataagt tttggaactg tcagtcgctc tctgtaataa tatgggtcta ttatagagag
     1141 aactttctca cctatcggag aatcagccag tttatccagg tccagagagg tgtagtcata
     1201 aagttttaag ctgaggtcct gccatatctc tcgctggtgg ctgaactgag cctttatgtt
     1261 caggttatca tacaccatcg gggctatacc ctttaccctt ccatcacagg cacccgttag
     1321 ccaggtggtc caccctctct tggatgcacc ggtaacaaga aaaccttcta tatcaatatt
     1381 aagctctctt tttgagaact cctgagtggc ggtcatagcc cttaccgcac tctttgccat
     1441 aggcaatagt attggccatt cctcatcgcc cgtttctaca tacctggcaa gggtataggc
     1501 tatcaatgca tcttctctca atccaccaaa cagaggttga ttgggtacat caaataaaac
     1561 agcagcaaca catcccagat ttctggtcat ctcttttata tagaggacct cctcgctatc
     1621 atatcctccc cagctacccg ttataaaaag cacgcctgtt tttggattaa taatagtttt
     1681 tgggacacct atcacaagtc tatgagacca gactatgtcc ttccatctct gagagaccag
     1741 gttgatatta aagatagtcg ccccttctac atcctccctg gatataatct cccagctaaa
     1801 gcacccatca tctttaccta cataatctat aagagtctgc ccataggcat ttgaaattaa
     1861 aaagatgaag agggaagtaa gtaccagtat ctttaccatc ttcttcatgc caatcacctc
     1921 ctgtttatat tttaacactt tttttagaaa ttttgcatac agaaatcctg tatgttaata
     1981 tagattcata tcaaagttat aaggagagag atatggagat tccagagctg ataaaggtta
     2041 gacagttttt taacagagag catatagag
//
LOCUS       DAOTKU010000309         2068 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_43516_length_2068_cov_2.547442, whole genome shotgun sequence.
ACCESSION   DAOTKU010000309 DAOTKU010000000
VERSION     DAOTKU010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43516_length_2068_cov_2.547442"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="P5310_07035"
     CDS             <1..220
                     /locus_tag="P5310_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRU33151.1"
                     /translation="DDIESLSQVYPFLTVVTQPAYSMGVIAAELLIRRIEGRDKIEER
                     RKVILQPELIVRESAGEKLAEGVRGEGR"
     gene            301..>2068
                     /locus_tag="P5310_07040"
     CDS             301..>2068
                     /locus_tag="P5310_07040"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRU33152.1"
                     /translation="MKRSLLVGLTLMLVVTLMLGSMPISFAQKIPTMPSPSQYNSPVE
                     YQRATGKKIARFNESPMLAELVKTGKLPPVEKRLPSEPLVIVPVEEVGQYGGTARVFS
                     NSLTGYGEAECLIGFEGLVKLGPDTTSVIPNIAKSWKYTDGGKTLTIYLRKGIKWSDG
                     VPFTADDILFWWEDVVLNDELTPVKPVTWSPGGKLMTVEKVDNYTIRMKFSVSHPFAI
                     LYLAHGWGTQASCYYPKHYSKKFHPKYTPIEELNKRAKAEGFDNWWQLFGKYNGFFGG
                     GGANVEIGAPTLQAFMVAKKGIDYLMAERNPYYWKIDIAGNQLPYIDRIQVGIVTDTE
                     MMNMKAITGEADYACMQTKLENYTLYKENEKKGNYRVLLWQRVQGADVAYQCNLTYKD
                     DTVLRDIFRDVRFRRALSLAINRDEINQMLYYGMATPRQACPIPSSPYFEEQFANAYI
                     KYDPKEANRLLDDMGLKRGPDGYRLRPDGKRLEILLEFYQTETPKRQTTELVQRYWDA
                     IGIKVAVKEVTGELQSTRAMANLMQMSLWHADRCGFLFPLEPFWWVPMRHQWEVTWCP
                     LWSQWYLSGGKAGEEPPKEAKNL"
BASE COUNT          691 a          317 c          519 g          541 t
ORIGIN      
        1 tgatgatata gagagtctat cacaggtata tccattctta acagtggtaa ctcagcccgc
       61 ctattcgatg ggtgtgatag cagcagaatt actgataagg cggatagaag gaagagataa
      121 gatagaggag aggagaaaag taatactcca gccagagctg atagtgagag agtcagctgg
      181 agagaaactg gcagaggggg tgagagggga gggaagatag agttttagtc gaaaatattt
      241 tttgaggggg ttgggtgaag aggatagaat atggagttaa aataaagagg aggctaaaaa
      301 atgaaaagaa gtttattggt aggtttaaca ttaatgctgg ttgttacttt aatgttagga
      361 agtatgccta tttcttttgc tcaaaagata ccaacaatgc catctccatc acagtataat
      421 tctcccgttg agtatcagag agcaactggc aaaaagatag caagattcaa tgaatcacca
      481 atgttagccg agctggtaaa aacagggaag cttccaccag tagagaaaag attaccatct
      541 gaaccacttg taatagttcc ggtagaagag gtaggtcaat atggtgggac ggcaagagta
      601 ttttctaatt ctcttacagg ctatggagaa gctgagtgtc ttatagggtt cgaggggctt
      661 gttaaattag gtcctgatac aacctcagta attcctaata tagcaaaaag ttggaaatat
      721 acagatggag ggaaaacact tacaatttac ctgcgtaaag ggataaaatg gtctgatggt
      781 gttcctttta ccgctgatga tatactcttc tggtgggaag atgtggtatt aaacgatgaa
      841 cttacaccag taaagcctgt tacctggtca cccggtggta agcttatgac tgtagagaag
      901 gtagataatt atactatcag gatgaagttt tctgtttcac atccatttgc aatcctttac
      961 ctggctcatg ggtggggaac acaggcaagt tgttactatc ctaaacacta ttccaaaaaa
     1021 ttccacccta aatatacacc aatagaggaa ttaaataaga gggctaaagc tgaaggattt
     1081 gacaattggt ggcagctttt tggaaaatac aacggatttt ttggtggagg cggggctaat
     1141 gtagaaatag gagcacctac cctgcaggca tttatggtag caaagaaagg tatagattac
     1201 cttatggcag agaggaatcc ttactattgg aagatagata tagctggaaa ccagttacct
     1261 tacatagata ggatacaagt aggtatagtt acagatacag agatgatgaa tatgaaagct
     1321 ataacaggag aagctgacta tgcatgtatg cagacgaagc tagagaatta tacactctat
     1381 aaagagaatg agaagaaagg taactatagg gttctgctct ggcagagggt ccaaggggca
     1441 gatgtagcgt atcagtgtaa tctaacatat aaggatgata ctgtgcttag agatattttt
     1501 agagacgtaa ggtttagaag agctctatct ctggcaataa atcgtgatga gataaatcag
     1561 atgttatact atgggatggc tactcctaga caagcttgtc cgatacctag ttccccttac
     1621 tttgaagaac agttcgccaa tgcatatata aagtatgatc caaaagaagc aaataggctt
     1681 ttagatgata tgggtcttaa aagaggacca gacggttatc gattaagacc agatggaaaa
     1741 cgattagaga tacttctgga gttttatcag acagagactc caaaacgtca gactactgag
     1801 ttagtgcaga gatattggga tgcaatcgga attaaggtag cagttaaaga ggtaacagga
     1861 gaattacaat ctacaagagc tatggctaat ctaatgcaga tgagcctatg gcatgctgat
     1921 aggtgtggct tcttgttccc cttagagcca ttctggtggg ttcctatgag acatcaatgg
     1981 gaagtaacat ggtgtcctct atggtcacaa tggtatctga gtggaggcaa ggctggggaa
     2041 gagcctccta aagaagcgaa aaatctta
//
LOCUS       DAOTKU010000310         2067 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_43531_length_2067_cov_4.441352, whole genome shotgun sequence.
ACCESSION   DAOTKU010000310 DAOTKU010000000
VERSION     DAOTKU010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43531_length_2067_cov_4.441352"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(234..>2067)
                     /locus_tag="P5310_07045"
     CDS             complement(234..>2067)
                     /locus_tag="P5310_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRU33153.1"
                     /translation="TQVAPQIPRNETIIVDILGGRVVNPKRFNSWVIGVSSGTDSGIQ
                     QLMLEPLWCVEYAKGRVLNVLAKEPPIYNKDFTQMTVKLREGIYWSDGVEFTADDVIF
                     TVETLKNKPGMSYNTEFNMYVDKISKTGKYTVIFDLKQPNSRFHTYFLDRWGACRFMP
                     KHIFEKVEDPLKFDFYPPISLGPYVLKDVDPAGYWRLWERRKDWNRTAVGKMYGMPKP
                     KYVLFIYYGAPEKKAMAQIKHELDMCDLTMESFRAVLQQNQYARGYRKDFPWVVNIDP
                     CITGITLNNAVSPFNIKDVRWALTLAIDIVDTIGIAFDGAATMGALLIPPTPAYLRWY
                     YRPMETWLKNFALDIEVDGKPFKPYDTNAPYRLAEYCKKRGYTVPPESQIKNVFGYGW
                     WKYAPEVATKLLERQGFKKGADGKWLLPDGKPWKITITTGTNPAHPQYRNGFAVAQQW
                     RKFGIDVEVKTSETAGTEGTYGKYEAITTWPAQEPWGGHPDLYRTFSAWRSKYVKPVG
                     EAAIGAAGYISRWSDKKLDGIIDDMEKTAWDDPNLTKLGIEGLKILVEEMPSIPTFGY
                     PGVVGWDEYYWTNYPGAENSYQQPYHHWPNFKFMLPYLKSTGRK"
BASE COUNT          523 a          480 c          388 g          676 t
ORIGIN      
        1 cagcatccgg gtctatataa gtcccctgca tcgtaactct ttctatagtc tgctggattg
       61 ggtctgttgg cataagtctt gggactacaa atactacggt tgtgcctatg aatattacca
      121 caagatactg gataatcctc ggtaacaggt atcttttaaa gaatgtcact ttttctgcac
      181 ctccattggg tatgctaaga gggctcctat tataggagcc ctcttagtta tttttacttt
      241 cttcccgttg atttcaggta tggaagcata aacttaaagt ttggccaatg gtgatatggc
      301 tgttgatatg aattttctgc accaggataa ttagtccagt aatattcatc ccatccaaca
      361 acgccaggat aaccaaatgt cggtatagac ggcatctctt cgaccaggat cttaagtccc
      421 tcgatcccaa gttttgtaag gttaggatca tcccatgcag tcttttccat atcatctata
      481 ataccatcca gtttcttatc agaccatctg gatatatatc cggcagctcc tatagcggct
      541 tctccaactg gctttacata ttttgaacgc caggctgaga atgttctata taagtcagga
      601 tgtcctcccc agggttcctg tgctggccag gtggtaatgg cttcatattt gccataagtg
      661 ccttcggtcc cagccgtttc tgatgtttta acttctacgt ctatgccaaa tttcctccac
      721 tgttgagcga cagcaaagcc atttctatat tgtggatgtg cagggttggt accggtagtt
      781 atcgtgattt tccagggttt tccatcaggt agcagccatt ttccgtcagc accttttttg
      841 aatccctgtc tctccaatag ctttgtcgct acctctggtg catatttcca ccagccataa
      901 ccaaatacat ttttgatctg gctctctgga ggtactgtat atcctctctt cttacaatat
      961 tctgcaagtc tataaggtgc gtttgtatca tatggcttga aaggctttcc gtcaacttct
     1021 atatctaaag caaaattctt aagccaggtt tccatcggtc tatagtacca tcttagatac
     1081 gctggcgttg gtggtataag caacgcaccc atcgttgctg caccgtcaaa tgctattccg
     1141 atggtatcca ctatgtctat agcgagggtt aaagcccatc tgacatcttt tatattaaat
     1201 ggagatacag cattgttaag agtgatacct gttatacagg gatcaatatt tactacccat
     1261 gggaagtcct ttctataacc tcttgcatac tggttctgtt gcaataccgc tctgaatgac
     1321 tccatagtaa gatcacacat gtcaagttca tgctttatct gtgccatagc ctttttctca
     1381 ggagctccat agtagataaa aagaacatac ttaggttttg gcattccgta catctttccg
     1441 acagctgttc tgttccagtc ttttctcctc tcccagagtc tccagtatcc tgcagggtct
     1501 acatccttta gaacatatgg tcccaggctt ataggaggat agaagtcaaa ttttaggggg
     1561 tcttcaacct tttcaaagat atgtttcggc ataaagcggc aggcacccca tctatccagg
     1621 aagtaggtat gaaatctgga gttcggctgc tttaaatcaa aaatcactgt atatttgcct
     1681 gtcttggata tcttgtccac atacatatta aattcagtat tgtaagacat tccaggttta
     1741 ttctttaatg tctccacagt aaatataacg tcgtctgctg taaactcaac accatcactc
     1801 caataaatgc cttctcttag ttttacagtc atctgggtaa aatctttatt atagattgga
     1861 ggttctttgg ccagaacatt aagtacccta cctttagcat attctacaca ccacagtggc
     1921 tctaacatca attgctgaat cccagaatct gtcccggaac ttaccccaat gacccaggaa
     1981 ttaaatctct tggggtttac tactcttcct cccaggatgt ccacaataat agtctcattc
     2041 cttggtatct gcggggctac ttgtgta
//
LOCUS       DAOTKU010000311         2065 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_43591_length_2065_cov_2.805473, whole genome shotgun sequence.
ACCESSION   DAOTKU010000311 DAOTKU010000000
VERSION     DAOTKU010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43591_length_2065_cov_2.805473"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="P5310_07050"
     CDS             <1..555
                     /locus_tag="P5310_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU33154.1"
                     /translation="LRHIGLNGLISCQIQRVFFPTGLPMYVMSRTLWDDSVAFDKIVE
                     EYFSDAFGPDGKLLKEYLERLSSLFTLILSMGEYSSSLESIDRLAEIREVVENFRPVI
                     EKNVSLRTGNIARSWLYIKYHREVVLRLISILEGIFKNEPDLASKEWQELKHFLQKEE
                     DVLQPVFDLFEFISVFERVLKISQ"
     gene            complement(545..1309)
                     /locus_tag="P5310_07055"
     CDS             complement(545..1309)
                     /locus_tag="P5310_07055"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HRU33155.1"
                     /translation="MIAGLQLYTLREYCKTPKDIADSLKKVKEIGYNVVQISGIGPID
                     TKELKKMLDDLGIKAVTTHLPYQRIINETEEVIEEHKILSATYVVCPGLPRELHNAEG
                     YKKVAGELNRAGEVMKKEGLVLLYHNHGIEFERYNKKLGLEILFENANPDILGMEIDT
                     YWVQYGGGDPAYWIEKFTNRMPICHFKDMGIKDNTQIMMPIGEGNLNWQAILKACEKA
                     GVTYAMVELDQAPLYSIWDAIKISLENMKSWGITTD"
     gene            1407..1492
                     /locus_tag="P5310_07060"
     tRNA            1407..1492
                     /locus_tag="P5310_07060"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1441..1443,aa:Leu,seq:gag)
     gene            1531..1608
                     /locus_tag="P5310_07065"
     tRNA            1531..1608
                     /locus_tag="P5310_07065"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1566..1568,aa:Asp,seq:gtc)
     gene            1618..1693
                     /locus_tag="P5310_07070"
     tRNA            1618..1693
                     /locus_tag="P5310_07070"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1651..1653,aa:Phe,seq:gaa)
     gene            1719..>2065
                     /gene="yfcE"
                     /locus_tag="P5310_07075"
     CDS             1719..>2065
                     /gene="yfcE"
                     /locus_tag="P5310_07075"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF006988.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HRU33156.1"
                     /translation="MSLIGITSDIHGSKTAWDAVFYSLRFKDVILWIIAGDILYHGPK
                     NPIPEGYSPKELAAAINSYGKPLIIVKGNCDSEVDQSLLKIPILSPYGVLFIDELKIL
                     IHHGDKDLNSWIFS"
BASE COUNT          552 a          426 c          415 g          672 t
ORIGIN      
        1 ctaagacaca taggattgaa cggtctgatc agctgccaga ttcaaagggt attctttcct
       61 acaggacttc ctatgtatgt gatgtccagg acactctggg atgatagtgt tgcctttgat
      121 aaaatagttg aagaatattt ttctgatgcc tttggtcctg acggtaaatt attaaaagaa
      181 tacctggaaa gactttccag cctttttact cttatcttat ctatgggaga atattcctcc
      241 tctctggagt ctatcgatag attggctgag ataagagaag tggttgaaaa cttcagaccg
      301 gtgatagaga agaatgtatc tctaagaaca ggaaatatcg ccaggtcctg gttatatata
      361 aagtaccata gagaagtggt attgagactg atttcaatat tggagggaat ttttaagaac
      421 gagcctgacc tggcttcaaa agaatggcag gagcttaaac attttctcca gaaagaggag
      481 gacgttctgc aaccagtatt cgatttgttc gagtttataa gcgtctttga gagagtccta
      541 aagatcagtc agtagttata ccccagcttt tcatattttc cagactgatc ttgatggcgt
      601 cccatatcga atagagaggt gcttgatcta actcaaccat agcgtatgtt actcctgctt
      661 tttcgcacgc cttgagtata gcttgccagt taagattacc ctctcctatc ggcatcatta
      721 tctgggtatt atcctttatt cccatatctt taaaatggca tatcggcatc ctattggtaa
      781 atttttctat ccagtaagcc gggtctcctc caccatactg aacccagtat gtgtctatct
      841 ccatacccag aatatcagga tttgcgtttt caaacagaat ctccaaacct aatttcttgt
      901 tatacctttc gaattctata ccatgattgt ggtacagtaa taccaatccc tccttcttca
      961 taacctctcc tgctctgttt aattcccctg ctactttttt ataaccctct gcattgtgca
     1021 attctcttgg taaccccgga catacaacat aagttgcaga taagatttta tgttcctcta
     1081 ttacctcctc tgtctcattt ataatcctct gatagggtag atgggttgtt actgctttta
     1141 tccccaggtc gtccagcatc ttttttaact ctttcgtatc tatcggtcct atgccggaga
     1201 tctgaactac attataacct atttctttta cttttttcag ggaatctgct atatctttag
     1261 gcgttttgca gtattccctc agtgtataga gttgaagtcc tgctatcatt tcccaatacc
     1321 tccttttagt tttctctgtt gactagatta tacataaaag gtacggtatt gacaagtcca
     1381 ctggatttag aatataatat agacacgccg aggtggcgga attggcagac gcgtacgttt
     1441 gaggggcgta tgggcgttcc ccgtgagggt tcgaacccct cccgcggcac cattgacaaa
     1501 atttttatat actggtaaaa tagataatac ggggccgtgg tgtagccggt ttagcacacc
     1561 tgcctgtcac gcaggagatc gcgagttcga atctcgtcgg tcccgccata ttgaaaagcc
     1621 gagatagctc agttggtaga gcagtggact gaaaatccac gtgtccccag ttcgactctg
     1681 ggtctcggca ccattaattt gtattgagga gtgaatactt gagcttaata ggtattacca
     1741 gtgatatcca tggtagtaag acagcatggg atgctgtctt ttattctttg agatttaaag
     1801 atgtcatcct ttggataatc gcaggagata tattatacca cggtcctaaa aatcccattc
     1861 cagagggata ttctccaaag gaattggcag ctgccataaa cagctatggt aaacctttaa
     1921 tcatagtaaa gggtaattgt gatagtgagg tggatcaatc tttattaaag attcctatcc
     1981 tttcccccta tggtgttctt tttatagatg agttaaaaat tcttattcat catggagata
     2041 aagatctgaa tagctggata ttttc
//
LOCUS       DAOTKU010000312         2063 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_43649_length_2063_cov_4.076693, whole genome shotgun sequence.
ACCESSION   DAOTKU010000312 DAOTKU010000000
VERSION     DAOTKU010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43649_length_2063_cov_4.076693"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(87..1469)
                     /gene="cysS"
                     /locus_tag="P5310_07080"
     CDS             complement(87..1469)
                     /gene="cysS"
                     /locus_tag="P5310_07080"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007546779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HRU33157.1"
                     /translation="MRLYNTLTRNEDEFIPRKRKMARMYVCGVTPYDYCHVGHGRSYL
                     IYDVLYRFLRSEGFSVLWIQNFTDIDDKIINRANELNIPPKELADKFISEYFVDMDRF
                     GISRAFLYPRVTEHIDEIVEFISKVVEKGYAYESEGDVYFSVRDYPSYGKLSRRSLEE
                     MIAGARVDPSEKKKDPMDFALWKSAKPGEPYWDSPWGRGRPGWHIECSVMSLLYLGEE
                     IDIHGGGADLIFPHHENELAQSEAVVGDGKFVRFWTHNGLVNLRSEKMSKSTGNFIIL
                     REILNSYRPEAFRLYLLGTHYRSPLDFDPQALEDAQRAYTRIEDTLKLLSHIPDPPSP
                     AYDQAFWDFRDALSNDLNTPQAIGTLFSYIRENRHLLDKGNWDPTLPIFKFNLIRMCE
                     ILGFKIEEKTSPDLESDKLREVMDIIIKIRNKLREEKLFHLSDEIRDSLNSIGVILED
                     MKEGTRWKMS"
     gene            complement(1466..>2063)
                     /locus_tag="P5310_07085"
     CDS             complement(1466..>2063)
                     /locus_tag="P5310_07085"
                     /inference="COORDINATES: protein motif:HMM:NF039902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU33158.1"
                     /translation="ALLGWSPKEEEVKTLEELIEEFSLEDVSPSPSAYDPAKLRWINR
                     QHILRLDGEKLLSYARPFLKEFENFPKEWLIKAILSIREYAETLKDIPEEIKKYYLND
                     ISLDDKYREELLSGKKAIEVFLDLLKNAEDISDIDSLFKEAIKVSGLKGRRFYHPIRI
                     ALTGYESGPELVELIPILGKRKCIERLENSLRFLEAEK"
BASE COUNT          532 a          478 c          345 g          708 t
ORIGIN      
        1 gggaaccagc ctcttttgaa tcagtatctt ttgtatcttt acccctgatt ttatagcttc
       61 tgatactgag tttttcccct ctatttttag ctcattttcc acctggtacc ctctttcata
      121 tcttctaata tgacacctat actgttcagg ctatccctga tctcatcact cagatggaat
      181 agcttctcct ctctcaattt attccttatt ttgataataa tatccatcac ttctctgagc
      241 ttatctgatt ccaaatctgg actggtcttt tcttctatct taaatcctaa aatttcgcac
      301 atcctgatga ggttaaattt aaatatcggt agcgttggat cccagttgcc cttatccagt
      361 aagtgtctat tctctcttat ataggagaac agggtaccaa ttgcctgtgg agtattcagg
      421 tcattggaga gggcatctct gaaatcccaa aatgcctggt cataggctgg agaaggtgga
      481 tcagggatgt gggatagaag ctttaaagta tcctctattc gcgtataagc cctctgtgca
      541 tcttcaagtg cctgggggtc aaaatccaga ggacttctgt agtgagtccc aaggagatat
      601 aatctgaagg cttctggtct gtatgaattt aagatctctc taaggattat aaaatttcct
      661 gtagattttg acatcttctc tgaccttaaa tttaccagcc cattatgagt ccagaatctg
      721 acgaatttgc catctccaac caccgcttca gattgggcta attcattctc atgatgtggg
      781 aagatgagat ctgctccacc accgtgtata tctatctcct cccccaggta caggagagac
      841 ataaccgagc attctatatg ccagccgggt ctccctcttc cccatggact atcccagtag
      901 ggctctccgg gcttagctga tttccagagc gcaaaatcca tagggtcctt tttcttttca
      961 cttggatcaa ccctggctcc agctatcatc tcttcaaggg aacgcctcga gagttttcca
     1021 tacgatggat aatctcttac agagaaatac acatctcctt ctgattcgta tgcataaccc
     1081 ttttctacaa cttttgatat gaactccact atctcatcta tatgctcggt aactctggga
     1141 tataagaatg ctctgcttat accaaatcta tccatatcca caaaatactc tgagataaat
     1201 ttatctgcca gttcttttgg aggtatattc aattcatttg ccctatttat tattttatcg
     1261 tctatgtcgg tgaagttctg aatccagagg acactgaatc cttcagatct tagaaatctg
     1321 tataacacgt catagatcag gtaggatctt ccatgtccca cgtgacagta atcatacggg
     1381 gttactccac atacgtacat ccttgccatc ttacgctttc taggtataaa ttcatcttca
     1441 tttctagtca atgtattgta taatctcatt tctcagcctc caaaaatctt aaagagttct
     1501 ctaatctttc aatacacttt ctctttccaa gtattggaat aagttctact aattctggtc
     1561 cagactcata ccctgtaaga gctattctta tcggatggta gaatcttctt cccttaagtc
     1621 ctgaaacctt tatagcctct ttgaacagac tatctatatc agaaatatcc tctgcattct
     1681 tcagtaaatc taagaatacc tctatagctt tcttgccaga caggagttct tctctgtatt
     1741 tatcatccag ggatatatcg tttagatagt atttttttat ctcctctgga atatctttaa
     1801 gtgtttctgc atattctctt atagaaagaa tagcttttat aagccattct ttaggaaagt
     1861 tctcaaattc ttttaagaaa ggtcttgcat aacttaacag cttctctccg tcaagtctca
     1921 gtatatgctg tctgttaatc catctcagtt tggcagggtc atacgctgat gggctgggtg
     1981 aaacatcttc tagagagaac tcttcaatta attcctctag agtctttact tcctcctcct
     2041 tgggactcca tccgagtaga gcc
//
LOCUS       DAOTKU010000313         2060 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_43742_length_2060_cov_2.675810, whole genome shotgun sequence.
ACCESSION   DAOTKU010000313 DAOTKU010000000
VERSION     DAOTKU010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43742_length_2060_cov_2.675810"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="P5310_07090"
     CDS             complement(<1..376)
                     /locus_tag="P5310_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRU33159.1"
                     /translation="MADEENVKKGKMVEEELESSLEEKVYLASQWQLIWWRFRRHKLA
                     IISGIILIIIYLLAIFCEFLAPYDPNKYDIKYTYAPPQTIHIFRDGKLVRPFVYGYKM
                     ELDPVTLRRVYTSDPEQVYPIYF"
     gene            complement(389..1372)
                     /locus_tag="P5310_07095"
     CDS             complement(389..1372)
                     /locus_tag="P5310_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004082548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRU33160.1"
                     /translation="MLQYVLRRILYMIPTLILIAVVSFIIIQLPPGDFLTSYIAQLSQ
                     TGETVDEATIAALRKRYGLDQPYYVQFFMWVWGMLHGDFGQSFEWNRPVSQLIWERLA
                     LTVAVSTFTMVFTWILSFPIGVYSATHQYSGGDYFFTFLGYIGLATPNFMIALILMWI
                     AYSVFGASVGGLFSPQYVEAAWSWGKFLDMLNHLWIPAIVIGMGGTAGLIRTLRANLL
                     DELHKPYVVTARAKGVPERTLLWRYPLRIAIIPWISTVGWSLPGIISGETITAVVLSL
                     PTTGPLLLRALQSQDMYLAGSFIMLLSFLTVIGTLISDILLGWVDPRIRYE"
     gene            complement(1462..>2060)
                     /locus_tag="P5310_07100"
     CDS             complement(1462..>2060)
                     /locus_tag="P5310_07100"
                     /inference="COORDINATES: protein motif:HMM:NF013843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HRU33161.1"
                     /translation="RFILGISGNIGAGKTTVVEILARHLGWEFYKEPAEKNPYLKPYY
                     EDPSRWGLHSQVYFLTSRFEEERRLSNLNKSYILDRTIYEDGEIFARIALKGDEYKTY
                     LKLYRLAINYLPGPDLIIHLKAPIDVLIDRIKKRGREEESRISYQYLSTLDSLYENWI
                     RTFKRVPVVIYNIERDINEKAEFIRLLNFIEESLKNRL"
BASE COUNT          572 a          487 c          366 g          635 t
ORIGIN      
        1 agaagtatat tgggtatacc tgttcagggt ctgatgtgta tacccttctg agtgttaccg
       61 ggtctagttc catcttatat ccatatacga atggtcttac cagtttccca tctctaaata
      121 tatggattgt ctggggtgga gcataggtat atttgatatc atatttatta gggtcgtatg
      181 gggcaagaaa ctcacagaaa atagccagca gatagataat aattaagata atcccgctta
      241 ttatagctag cttatgcctt ctaaacctcc accatataag ttgccattgg gatgctaaat
      301 atactttttc ctctagagag ctttctagct cctcttctac cattttccct tttttgacat
      361 tttcttcatc cgccattctt ctccctcctt actcgtacct tatcctagga tcaacccaac
      421 ccaggaggat atctgatatg agagttccta taacagttaa gaagctcaag agcataatga
      481 aacttcctgc cagatacata tcctgacttt gaagagctct taacagaaga ggtcctgtag
      541 tcgggagact taatactact gctgttatag tctcaccaga gattattcca gggagtgacc
      601 acccgacagt acttatccag gggattatcg ctatcctaag tggatacctc cacagaagag
      661 ttctctctgg aaccccttta gcccttgctg ttaccacata gggtttgtgc aactcatcta
      721 agaggttggc tctcaaggtc cttatcaatc ctgcagttcc acccatacct attactattg
      781 ccggtatcca cagatggttt agcatatcca ggaatttacc ccagctccag gcggcctcta
      841 catactgagg tgagaataac ccacctacgc tggctccaaa caccgagtac gctatccaca
      901 taaggatcag agctatcata aagtttgggg tagctaaccc tatgtaacct agaaaggtaa
      961 agaaataatc accacctgaa tactggtgtg ttgcagaata cacacctata gggaaagaca
     1021 gtatccaggt gaacaccatg gtaaacgttg acactgcaac agtaagagct aatctttccc
     1081 agatcagctg actcacaggt ctattccatt cgaaagactg cccaaaatcc ccatggagca
     1141 tcccccaaac ccacatgaaa aactgaacat agtacggttg atccaatccg taccttttcc
     1201 ttagagctgc tatagttgcc tcatccactg tctcccctgt ctgacttaac tgtgctatat
     1261 aagaggttaa gaaatcgcca gggggtaact ggatgatgat gaatgatacc actgctatta
     1321 agatcagcgt gggtatcata tacaggatac gtctcaatac atattgaagc attctttccc
     1381 tcctttaccg tatggttgtt ttatcgctat aaatcgcttg taatttagaa tataccacat
     1441 acctctttag ctgtcaatca gttatagcct attttttaaa ctttcttcta taaaattcag
     1501 cagtctgata aattctgcct tttcatttat atctctctct atattataaa ttactacagg
     1561 gactctttta aatgttcgta tccagttctc atacaatgaa tccagcgtgg atagatactg
     1621 ataagatatt cttgattcct cttcccgtcc ccgtttcttt attctatcta tcagaacatc
     1681 tataggagcc tttagatgta ttattaagtc tggacctggc agataattta tcgccagtct
     1741 ataaagcttc agatacgtct tatactcatc accctttaga gctatcctgg caaagatctc
     1801 accatcttca tatatagttc tgtctagtat ataggactta ttaagattgc ttaacctcct
     1861 ctcctcctca aacctgctgg tcaagaaata gacctgggaa tgaagtcccc atcgagaggg
     1921 gtcttcatag tagggtttaa ggtacggatt cttctctgct ggttccttat agaactccca
     1981 tcccagatgc ctggcgagta tctctactac ggtggtttta cccgctccta tattacctga
     2041 aattccaagg atgaatcttc
//
LOCUS       DAOTKU010000314         2059 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_43787_length_2059_cov_2.219561, whole genome shotgun sequence.
ACCESSION   DAOTKU010000314 DAOTKU010000000
VERSION     DAOTKU010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43787_length_2059_cov_2.219561"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1678
                     /locus_tag="P5310_07105"
     CDS             <1..1678
                     /locus_tag="P5310_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU33162.1"
                     /translation="KKVINIAYTAENIRNADWTWYFYDGSKFTQDPVSLYNCQFTELF
                     LYTYKLTDDKKYMDFARKTIYALPELLYFIGSTAAYNYFFYPFTMIAEYLYSTGENSA
                     ELKEAREYAYYMAMQGYNPNTKKWYYSPAEYIRGFYDGHSANYQLGEMASFLEHQDAI
                     RAIFPEEYNYFLNELPGMLDVVKRYQLPSGAYYYNNDAPDCTETIGGVISFYTLYDRT
                     FKTNHDDIIKRCIETILGRQAPNGAYYKTAGSDVAEIWYGDGIGLAIPLYLLMKENGK
                     KEDKVSEKPEAIKERKSREIILGDYDAELRDTSGRVDSVTLIKRLKSLGVNTYAYLIW
                     HSPDDWEDLCQSFMPLAQDEDINVIVYMVPPSEPPSPKPYEYDYIEWAQAIGKLSRRY
                     PGLIGIAIDDFNYNISFFTPSYLKEIKKTINDINPSLKLFTVSYYRDITDTFLAQYGE
                     IIDGIIFPFRNEPRVDTVTTFTLDPQVRSVRATLGDKLSVIIMIYASKLSSATIPPDA
                     RYLRETISTSLELIEEGIADGIITYCLPKVSEEDEMFKTVKELFSSFNKR"
     gene            1692..>2059
                     /locus_tag="P5310_07110"
     CDS             1692..>2059
                     /locus_tag="P5310_07110"
                     /inference="COORDINATES: protein motif:HMM:NF026221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU33163.1"
                     /translation="MGKWWEKPLRIVDIGLPEYKLERIAEAVKRKKELHFNCEHFSAF
                     GYEGGEKGIFYFKSSVAEQVQLDMLDEYLRAAHGEGIRVIIYFNVHWLSPAVANKHKD
                     WVQLRRDGSVIDDVYGLGMSP"
BASE COUNT          695 a          337 c          435 g          592 t
ORIGIN      
        1 aaaaaaggtg ataaatatag cttataccgc tgaaaacatt agaaatgcag attggacctg
       61 gtatttttat gatgggtcta agtttacgca agatccagta tccctttata attgccagtt
      121 taccgagctt tttctttata cctataaatt gactgacgat aaaaagtata tggactttgc
      181 cagaaagacc atctatgctc taccagaact cttatacttt ataggttcaa ctgctgcata
      241 taattacttc ttttatcctt tcacaatgat agcagagtat ctctacagta ctggagaaaa
      301 tagtgctgaa ttaaaagagg ccagagaata tgcctactat atggctatgc aaggttataa
      361 tcctaataca aagaaatggt actattcacc agctgaatat ataagaggat tctatgacgg
      421 tcatagtgct aactaccagt tgggagagat ggcatctttt cttgaacatc aggatgctat
      481 acgggctata ttcccagaag aatataatta tttcctgaat gaacttccag gtatgctgga
      541 tgtagtgaaa aggtatcagt taccatcagg tgcctattac tataataatg atgcgccaga
      601 ctgcactgag actattgggg gagtaatatc attctatact ctatatgata ggacatttaa
      661 gactaaccat gatgacatca ttaaaagatg tattgaaaca atacttggcc ggcaagcccc
      721 caatggagct tattacaaaa cagctggaag tgatgtggca gaaatctggt atggggatgg
      781 gattggtctt gccataccac tgtatcttct aatgaaagaa aatgggaaaa aggaggataa
      841 agtgtctgaa aaacctgagg ctattaaaga gagaaaaagt agagagatta ttctgggtga
      901 ttatgatgct gaactcagag atacatctgg aagagttgat agcgtaactc ttattaaaag
      961 acttaagtct ttgggggtaa atacatatgc ctatctgata tggcactctc cagatgactg
     1021 ggaagacctg tgccagagtt ttatgccttt agctcaagat gaagatatca acgtcatagt
     1081 atatatggta ccaccttctg aaccaccatc tccaaaacca tacgagtatg attatataga
     1141 atgggctcaa gctataggaa aattatctag aagatatcct ggacttatag gtatcgctat
     1201 agatgacttt aactataata tcagcttctt taccccctct tatcttaaag agataaagaa
     1261 aactattaat gacataaatc cttctttaaa attatttacc gtatcctact acagagatat
     1321 aactgataca tttctagccc aatatggaga gattatagat gggataatct ttccatttag
     1381 aaatgaacct agagtggata ctgtaactac attcacgtta gacccacagg taagatctgt
     1441 aagagcaaca ttaggagata aactatcggt aatcattatg atatatgcgt ctaaactgtc
     1501 tagtgccacc atacctccgg atgccagata tctaagagaa acaataagta cttctctgga
     1561 gcttatagag gaaggtatag ctgatggcat tattacttac tgtttaccta aggtgagcga
     1621 agaggatgag atgtttaaaa cagtaaaaga attattctcg agtttcaata aacggtagga
     1681 ggtacatacc tatgggaaag tggtgggaaa aacccctgcg tatagtagat ataggattac
     1741 ccgaatataa gctggaaagg attgcagaag cggtaaagag aaagaaagag ttacacttta
     1801 actgtgaaca tttcagtgct tttggttatg agggtggaga aaaaggcatc ttttatttta
     1861 aatccagtgt agctgaacag gtccagctgg atatgcttga cgaatatctg agagctgcac
     1921 acggagaggg gatcagggta ataatatatt ttaacgtcca ctggctgagt cctgcggttg
     1981 cgaataaaca taaagattgg gtacagctca gaagagatgg tagtgtaata gatgatgtct
     2041 atggtttagg aatgtctcc
//
LOCUS       DAOTKU010000315         2041 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_44281_length_2041_cov_3.550352, whole genome shotgun sequence.
ACCESSION   DAOTKU010000315 DAOTKU010000000
VERSION     DAOTKU010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44281_length_2041_cov_3.550352"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="P5310_07115"
     CDS             <1..205
                     /locus_tag="P5310_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRU33164.1"
                     /translation="ILIKAQQATSVSARKRLYEAAQEIIKSDAPWVTLAHTSAVAATR
                     KYVKNFAVPAIGAIKFTRVWLDK"
     gene            266..1270
                     /locus_tag="P5310_07120"
     CDS             266..1270
                     /locus_tag="P5310_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRU33165.1"
                     /translation="MYRYILRRLLIMIPVIIGVSAVVFFLIRSIPGDPALILLGEHSS
                     EEAVAKIREQLGLDKPKIVQFFYFLLNIAKGDFGRSFTTNELVVTEIAKHFPATIELT
                     ICSMIFGSIVGLVLGVTAATYRNSIIDYLTTVVSVTGVSMPIFWLGLMAIILFSIKLP
                     LFPISGRLDPTITFRGRTGLIILDSLLQGNFAVFKDAIHHIFLPALCLGTIPLASIAR
                     MTRSSMLEVLNQDFIRTAKAKGLPSRKVIYTHALRNALIPVVTVMGLQFGSLLGGAVL
                     TETIFAWPGIGRLSVEAIDARNYPVIQGCIIFFAFIFVIINLLVDILYAFIDPRIRYE
                     "
     gene            1285..>2041
                     /locus_tag="P5310_07125"
     CDS             1285..>2041
                     /locus_tag="P5310_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRU33166.1"
                     /translation="MRRSFKVFRNKAALAGLLTILFFIFIAVFAEFIAPYSPYEQNLA
                     DRLQPPSKSHLLGTDDLGRDILSRIIYGARISLAIGLISVALALGVGISAGLVSAYYG
                     GIVDTLLMRLFDVMLAFPTILLAMAIMAALGPDLENAMIAVGIVNIPTFARIVRASAL
                     IVKEQEYVMAAQALGQSNSKIIFRHILPNIVGPITVQVTLSYGGSILNAAGLGFLGMG
                     AQPPTPEWGAMLSQARSFIISAPWTVTFPGIAIF"
BASE COUNT          594 a          364 c          441 g          642 t
ORIGIN      
        1 tatacttata aaagctcaac aggctacatc tgtttctgcc agaaagagac tctatgaagc
       61 tgcgcaggaa ataataaaat cagatgctcc ctgggtaaca ttagctcata caagtgctgt
      121 ggcggctaca aggaaatatg taaagaactt tgctgttcca gctataggtg ctataaaatt
      181 taccagggta tggttggata agtaacagga tagaaactgg ggggtgagta agactcactc
      241 cccttatttt taaggaggaa cgcttttgta tagatacata cttagaaggc ttcttataat
      301 gatacctgta ataatagggg taagtgcagt agtattcttt cttataaggt caattccagg
      361 ggaccctgct ttaatcctcc ttggagaaca ttctagcgaa gaggcagtag caaagatacg
      421 tgagcagctg gggttggata aacccaagat tgttcagttc ttctattttc ttcttaatat
      481 agccaaaggt gactttggca ggtcttttac tacaaatgaa ttggtggtca cagagatagc
      541 aaaacatttt cctgccacca ttgaacttac aatttgcagt atgatatttg gttctattgt
      601 tggacttgtt ttaggtgtca cggctgcaac ctatagaaat tctattattg attatttgac
      661 cacggtagta tcagtaactg gtgtatcaat gccaatcttc tggcttggtt tgatggcaat
      721 aatactattt tcaataaaat tacctctctt cccaatttca ggaagacttg acccaacaat
      781 aacattcaga gggagaacag gtttaataat cctggattct ctcctccagg gaaactttgc
      841 tgtttttaaa gatgcaatac atcatatatt tctgccagct ctatgtttgg ggacaattcc
      901 attggcttct atagccagaa tgactcgttc cagtatgctg gaggttttaa atcaggattt
      961 cataaggaca gcaaaagcaa agggattgcc atccagaaag gtcatatata ctcatgcgct
     1021 gaggaatgca ttgattccag tagttactgt tatgggactt cagtttggta gtcttttagg
     1081 cggtgcagtt ctcactgaaa cgatatttgc ctggccaggt ataggtagat tatctgtaga
     1141 agccatagat gccagaaatt atccagttat tcagggttgt ataatattct ttgcctttat
     1201 attcgttatt attaatctct tagtagatat actttatgca tttatagacc caaggataag
     1261 gtatgaataa ggagagctga agagatgaga agatcattca aagtattcag gaataaagct
     1321 gcactggcag gtcttctaac aatcttattc tttatcttta ttgcggtatt tgcagaattc
     1381 attgccccat atagtccata tgagcagaac ctggctgaca gattacaacc accatccaag
     1441 agtcatctcc ttggtactga cgaccttgga agagatatac tttccaggat tatatatgga
     1501 gccagaatat ctctggctat aggattgatc tcggttgctt tagcattagg agtagggatt
     1561 tcagcaggat tggtatctgc ttattatgga gggatagtag atacattact tatgaggttg
     1621 tttgacgtta tgctagcttt tcccaccatt cttttagcta tggctattat ggcagcattg
     1681 ggtcctgatc tcgaaaatgc tatgatagcg gttggaatag tcaatattcc tacctttgca
     1741 agaatagtaa gggcatctgc actaatcgtt aaagaacaag aatatgtaat ggctgcccag
     1801 gcattaggac aatcaaattc caagattata tttagacata tacttccaaa tattgtcggt
     1861 ccaataactg ttcaggtaac attaagttat ggaggctcca tattgaatgc agctgggcta
     1921 ggctttttag ggatgggtgc tcagcctcca actcctgagt ggggagcgat gttgagtcag
     1981 gcaagaagtt ttatcatatc tgccccctgg acagtaacat ttccagggat agcgatattt
     2041 t
//
LOCUS       DAOTKU010000316         2035 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_44445_length_2035_cov_3.691414, whole genome shotgun sequence.
ACCESSION   DAOTKU010000316 DAOTKU010000000
VERSION     DAOTKU010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44445_length_2035_cov_3.691414"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="P5310_07130"
     CDS             <1..652
                     /locus_tag="P5310_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015756441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HRU33167.1"
                     /translation="ILLKAIIEKCNRLGKPVITATQMLDSMIRNPRPTRAEVSDVANA
                     IFDGTDAIMLSGETAIGEYPMESVLTMARIAEYTESKLKYSDMLRKRAEFSTNSPTDA
                     IGYATCEIAQRLGAKAIITSTQSGYTARMVAKYRPSQPIIAVTPDERIQRRLLMLWGV
                     SPIFTKIYNTTDEMIDASVQASLNAGLIRKGDTVILTGGIPVGIGGTTNFLKVHQV"
     gene            882..1265
                     /locus_tag="P5310_07135"
     CDS             882..1265
                     /locus_tag="P5310_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HRU33168.1"
                     /translation="MDKTWRDPVYSGTKPVENQIVEGRVKKITKFGAFLLLSDGREAF
                     LHISEIDSSYVKDVSDYLHEGDKVKAIVIGQNKKGELEVSIKRVKSLEEFENKLSHFL
                     KQSDEKLGDLKKSLEEKWKGKQKKW"
     gene            complement(1277..>2035)
                     /locus_tag="P5310_07140"
     CDS             complement(1277..>2035)
                     /locus_tag="P5310_07140"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRU33169.1"
                     /translation="SLIFFLISKFAFNPSSPVGGAVIYLYRINLILAGFNIIPAFPLD
                     GGRVLRAILWMLFKNFKKATRISAGIGITLATMIMFLGFYFILAGQLLSGLWFIFIGW
                     FLNQGAISGEREIDIKEAFVDAKVEDLMTRDVKSVPPNITIEQLVSDYLLSYKYVAFP
                     VMWGDVLLGIITLNDIKGLPREEWKNHTVREIMSPLSPDLVIGPGNSAFDAFLKINRG
                     PTGRLLVMDEDKLVGIISKTDLIRFLQIKSMLTD"
BASE COUNT          710 a          353 c          424 g          548 t
ORIGIN      
        1 tatacttcta aaggctataa tagaaaagtg caatagactg ggaaagccag taatcactgc
       61 tactcagatg ctagattcta tgatcagaaa tcccagacct accagggctg aagtctcaga
      121 tgtggcaaat gcgatctttg acggaacaga tgctatcatg ctatcaggtg aaacggcaat
      181 aggggagtat ccaatggaat ctgtcctcac aatggcaagg atagctgaat atactgagtc
      241 caagcttaaa tatagtgaca tgttaagaaa gagggcagaa ttttcgacta actctcccac
      301 agatgcaata ggttacgcca cctgtgagat agcccaaaga cttggagcaa aagcaataat
      361 tacatccaca cagtcaggtt atactgcacg tatggtagcc aagtatagac catcacagcc
      421 cattatagct gttacccctg atgaaaggat tcagagaaga ctattgatgc tctggggagt
      481 aagtccaata tttacaaaga tatataatac aacagatgag atgatagatg cttcagttca
      541 ggcatctttg aatgctggac ttataagaaa aggtgatacc gtgattctta ccggtggtat
      601 accggtaggg ataggtggaa ccacaaactt tttgaaggtg catcaggttt gaggaaatca
      661 gaaaaaatta tccttttatt ttttataatt gcatttctgg gaatcacaac aaggtctacc
      721 tttattatcc taaaagagat aaaggatagc gaaataagaa tctcccagct taaagagcat
      781 ttgaacaaac taagagatga gaatgttaaa ttaagagagg aaatctatag attaaaatat
      841 gataataatt atattgagat tctggtaaga gaggagttag gatggataag acctggagag
      901 atcctgtgta ttccggtaca aaaccagtag agaatcaaat cgtagaagga agagttaaga
      961 aaattactaa atttggtgca tttttgctac ttagcgatgg aagagaggca ttcttacata
     1021 tctctgagat agatagttct tatgtgaaag atgtctcaga ttatctccac gagggggata
     1081 aagtaaaagc tatagttata gggcaaaata agaaggggga attagaggtc tctataaaga
     1141 gagttaagtc tttagaagag ttcgagaaca aactgtccca ttttctaaaa cagagcgatg
     1201 agaaacttgg agatttaaag aaaagcctgg aagaaaagtg gaagggtaaa caaaagaagt
     1261 ggtaaatggc gttaagctaa tcagttagca tagactttat ctgtaagaac cgtataaggt
     1321 ccgtcttact tataataccc accagtttat cctcgtccat aacgagtaat ctgcctgtgg
     1381 gtcctctgtt tatcttaagg aaagcatcaa aagctgaatt tcctggacct attacaagat
     1441 caggagataa ggggctcatt atttctctaa ccgtatgatt cttccattct tctctgggaa
     1501 gtcctttaat atcattcaga gtaataatac caagaagtac atccccccac attacaggaa
     1561 aagccacata tttatatgag aggagatagt cagagacaag ctgttcaata gtgatatttg
     1621 gtggaacaga tttaacatcc ctggtcatga ggtcttccac tttagcatcc acaaaggctt
     1681 cttttatgtc tatttccctc tccccagata ttgcaccttg atttaagaac catcctataa
     1741 atataaacca gagaccactt aataattgac cggcaagaat gaaataaaat cccaagaaca
     1801 ttatcatagt tgcaagggtt attccaatac ctgctgatat tctggtagct tttttaaagt
     1861 tcttgaacag catccagagt atagctctca gcaccctacc accatcaagt ggaaaggctg
     1921 gaattatgtt aaatccagcc agaatcaggt ttatcctgta aagatagatt acagctcctc
     1981 ctacaggaga ggatggattg aaggcaaatt tagagattaa gaaaaaaatc aagga
//
LOCUS       DAOTKU010000317         2034 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_44484_length_2034_cov_2.840323, whole genome shotgun sequence.
ACCESSION   DAOTKU010000317 DAOTKU010000000
VERSION     DAOTKU010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44484_length_2034_cov_2.840323"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="P5310_07145"
     CDS             <1..685
                     /locus_tag="P5310_07145"
                     /inference="COORDINATES: protein motif:HMM:NF037182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HRU33170.1"
                     /translation="EDITHKLFPKARISRLDADLSEKEIEEIWKKFMEGHIDILVGTQ
                     MIARSFRLPNVYLAGILVPDTSLNIPDFRSSEKIFNLIYSLAEQAKDDKHVIIQTLNP
                     DYYPIKYAVRLDYKDFYQYEVRLRRTLGYPPYSRIIRIIVSGLEEEGVKTIISSLAQD
                     IERKLGISYSGPARAPLYKMRGRFYWHILLKIKDLNLDILKVVKEIINSYNYKGIDIM
                     VDVDPVSTV"
     gene            704..1168
                     /gene="def"
                     /locus_tag="P5310_07150"
     CDS             704..1168
                     /gene="def"
                     /locus_tag="P5310_07150"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HRU33171.1"
                     /translation="MSGLAIRIYGDPVLRQKALPIKNIDRRVRRLAKYMEEAMDKANG
                     VGLAAPQVGVSERIIVVKVKSPIVLVNPVITYMEGEDLAEEGCLSLPNVVGDVGRATR
                     VVVKGLDLNEREIEVEAKNLLARAFQHEIDHLDGILFIDRAVSIRRVEESSD"
     gene            1195..>2034
                     /gene="fmt"
                     /locus_tag="P5310_07155"
     CDS             1195..>2034
                     /gene="fmt"
                     /locus_tag="P5310_07155"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HRU33172.1"
                     /translation="MPSLEYLRDRISLVITRPDRPQGRGLKVLSTPVKSKAIELRIPV
                     LSPERLIDIKDKLTGFDLGVVVAYGLIIPRKIINLFPMGIINLHPSLLPKYRGAAPIQ
                     WALLNGEEETGVSIIYLTEELDAGDIIIQEKVKILPEDNAETLSSRLSVIGANVLAKA
                     VELIENNNVRPVPQNSLGIPSYAPKIEPQMGRIDWNTGARDIVNKVRALSLWPGAYFY
                     SKGIKVNMLEAEVSPINGEAGKVIDIGDNGILVGSRDGSVWIKKIRPENRKIMSGLDF
                     ANGY"
BASE COUNT          665 a          324 c          489 g          556 t
ORIGIN      
        1 agaagacata acgcataaat tatttcccaa agccagaata tccagacttg atgctgacct
       61 ttccgagaag gagatagaag agatatggaa gaaatttatg gaagggcata tagatatcct
      121 ggttggaacg cagatgatag ccagaagttt ccgtctaccg aatgtatatc tagctggtat
      181 cctggtacca gataccagtc tgaatattcc ggactttaga tccagcgaaa agatttttaa
      241 tctcatatat tctttagcag aacaggctaa agatgataaa cacgtaatta tccagactct
      301 gaatccagat tattatccaa taaaatatgc ggttagactg gattataaag atttttacca
      361 gtatgaagtt cgtttacgga ggacacttgg atatcctcca tacagtcgta taatcaggat
      421 aattgtctct ggactggaag aggagggagt aaagacgatc atttcctccc tggctcagga
      481 tatagaaaga aagctcggta tatcatactc tggtccagcc agggcaccct tatataagat
      541 gagaggaaga ttttactggc atatactttt gaagataaag gatctgaatc tggacatatt
      601 aaaagttgta aaagaaatta taaattccta taattataaa gggatagata taatggtgga
      661 tgtggatcct gtatctaccg tatagaattc aggaggtaaa gcaatgtcag gtttggctat
      721 aagaatatat ggggaccctg tcttaagaca gaaggcactc ccgataaaga atatagatag
      781 aagagtaaga agattagcta aatatatgga agaagctatg gataaggcta atggtgtggg
      841 attagctgcc cctcaggttg gagtgagtga gagaatcatt gtggtaaagg taaaatcccc
      901 tatagtactg gtaaatcctg tgattactta tatggaggga gaagatttag ctgaggaggg
      961 ttgcttaagt cttcccaatg tggtaggtga tgttggcagg gcaactagag ttgtggtgaa
     1021 aggtttggat ttaaatgaga gagagataga ggtggaggct aagaatcttc ttgccagagc
     1081 atttcagcat gagatagacc atctggacgg cattcttttt atagacaggg cggtatctat
     1141 caggagggtt gaagaatcat cagattagta ttctttggca ctccagagtt ttctataccg
     1201 tctttagagt atttacgaga taggatatct ctggttataa ctaggccgga cagacctcag
     1261 gggagagggc ttaaagtcct ttcaacacct gtaaaatcta aggctataga attacgtata
     1321 cctgtgcttt ctccagagcg tcttatcgat ataaaagata aattaaccgg gtttgacctt
     1381 ggagttgtgg ttgcatatgg acttattatc ccaagaaaaa tcataaacct gtttcccatg
     1441 ggaattataa atcttcatcc atccctatta cccaaataca gaggagcagc cccaattcag
     1501 tgggctctat taaatggaga ggaggagaca ggggtaagca taatatatct caccgaagag
     1561 ctggatgccg gggatatcat aatacaggag aaagtcaaaa tcctgccaga agataatgca
     1621 gaaaccttat cttcaagatt aagtgttatc ggagcgaatg ttctggcaaa agctgttgaa
     1681 ttgattgaaa ataataatgt cagacctgtc ccgcaaaatt ctttaggtat accatcatat
     1741 gcccccaaaa ttgagcctca gatgggtagg atagattgga atacaggtgc aagagatatt
     1801 gtaaataaag tcagagcact ttctctctgg ccaggagctt atttttacag taagggcatc
     1861 aaggttaata tgttggaggc tgaagtttcc cctattaacg gagaagcagg caaagtgatc
     1921 gatatagggg ataatggtat cctggttggg agtagagatg gatctgtgtg gattaaaaag
     1981 atacgaccgg aaaacagaaa gataatgagc gggttagatt tcgccaatgg atat
//
LOCUS       DAOTKU010000318         2024 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_44779_length_2024_cov_3.429152, whole genome shotgun sequence.
ACCESSION   DAOTKU010000318 DAOTKU010000000
VERSION     DAOTKU010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44779_length_2024_cov_3.429152"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="P5310_07160"
     CDS             complement(<1..545)
                     /locus_tag="P5310_07160"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HRU33173.1"
                     /translation="MRVIVTGGCGFMGSDFVRKIAGRGFDVVVVDKLTYAGDKNRLKD
                     IEGRYRLYINDITDFDTLKEIFEEEKPEYVVHYAAETHVDRSIIYPDAFVKTNVIGTF
                     NLLKLSLDYNIKKFIHISTDEVYGELPRDKGIKFTEESPLRGKSPYSASKAGADHLVQ
                     AFIKTYELPAVIIRASNNYGP"
     gene            complement(542..1108)
                     /gene="rfbC"
                     /locus_tag="P5310_07165"
     CDS             complement(542..1108)
                     /gene="rfbC"
                     /locus_tag="P5310_07165"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012674978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HRU33174.1"
                     /translation="MPFNFVKLEIPEVILVEPVAFSDERGFFMETYKASEFKRNGIDY
                     DFLQDNHSRSKRGVLRGLHYQLKPAEQGKLVRCIRGRIQDVAVDIRKGSPTYGRWVAV
                     ELSEDNKKMLWIPPGFLHGFVALEDDNEIIYKVTKEYSKEYDRGIIWNDPEIGINWGI
                     DNPVLSEKDKRLPRLKEAENNFIYQEEL"
     gene            complement(1098..1988)
                     /gene="rfbA"
                     /locus_tag="P5310_07170"
     CDS             complement(1098..1988)
                     /gene="rfbA"
                     /locus_tag="P5310_07170"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018962761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HRU33175.1"
                     /translation="MKGVILAGGTGTRLYPVTISTNKHLLPVYNKPMIYYPLSLMMLM
                     EIRELVIVINPEDKSQFEGLLGDGSNLGMNITYAIQPAPNGLAGGLLSAKESVEGENI
                     CLILGDNILYGHDVLKILNEAKRYLEDHNGAYVFGYHVPDPERFGVVEFDERGNVLSI
                     KEKPERPRSNFAVIGLYMYDRDVFEKISRIRPSERGELEITSLNEEYLKEGKLKVKLL
                     GRGFAWFDVGTHDSFLEGSEFVGTVEKRTGLMIGCVEEIAYRNGWIDRKQLLRLAERL
                     NKTDYGRYLQAIAEEEKDAI"
BASE COUNT          566 a          503 c          295 g          660 t
ORIGIN      
        1 cagggtccat aattattgga ggctctgatt atcactgctg gaagttcgta tgtcttaata
       61 aaagcctgaa caagatggtc tgctccagcc ttacttgcag agtaaggaga ttttcctctt
      121 aaagggcttt cctctgtgaa tttaataccc ttatcccttg gtaattctcc atagacctca
      181 tcggtagata tatggatgaa ctttttgata ttataatcta atgaaagctt tagcagatta
      241 aatgtaccaa tgacatttgt cttaacaaag gcatctggat agataatact tctatcaaca
      301 tgagtttcag cagcataatg aaccacatac tcaggctttt cttcttcaaa tatctctttt
      361 aaggtatcaa aatctgttat atcgttaata taaagtctat accttccttc tatatctttt
      421 aatctgtttt tatctccagc ataggtaagc ttgtccacaa ccaccacatc aaagccacgt
      481 cctgctatct tccttacaaa atcacttccc ataaatccac agccaccggt aacaatgact
      541 ctcataattc ctcctgatat ataaagttat tctctgcctc ttttaatcgg ggcaatctct
      601 tatccttttc agaaagtaca gggttatcta taccccagtt tataccaatc tcagggtcgt
      661 tccagatgat acccctgtca tattcttttg aatattcctt tgtaacctta tagattatct
      721 cattatcatc ttccagtgcc acaaagccat ggaggaaacc tggtggtatc catagcatct
      781 ttttattgtc ctcagagagc tccactgcaa cccacctgcc ataggtggga gagcctttcc
      841 ttatatcaac tgccacatcc tggattctcc ccctaataca tcttaccagc ttcccctgtt
      901 cggcaggttt cagttgatag tgaagtcctc tcaagacacc tcttttggac ctggagtgat
      961 tatcctgtag aaaatcataa tctatcccat ttcttttaaa ctcagatgcc ttatatgtct
     1021 ccataaaaaa gcctctctca tcagaaaaag ctacaggttc aacaaggata acttccggta
     1081 tctcaagctt aacaaagtta aatggcatct ttctcctcct cagctatagc ctgcaagtat
     1141 cttccataat cagttttatt caatctctct gcaagcctta aaagctgttt tctatctatc
     1201 catccgtttc tataagctat ctcctccaca catcctatca taagacctgt ccttttttct
     1261 acagttccca caaactcgct cccctcaaga aagctatcat gggttccaac atcaaaccag
     1321 gcaaagcctc ttcccagaag ttttacctta agttttccct ctttaagata ctcttcattc
     1381 aaggatgtta tttcaagttc tcctctttcc gaaggtctta ttctgcttat cttttcaaag
     1441 acatctctat catacatata gagacctata actgcaaaat tagacctggg tctttcaggc
     1501 ttctctttga tagataagac atttcctctc tcatcaaact caaccacccc aaacctttct
     1561 gggtctggca catgatagcc aaagacatat gcaccgttat ggtcttcaag gtacctcttt
     1621 gcttcattca atatcttcag aacatcatga ccatacagga tattgtctcc aagtataaga
     1681 cagatatttt caccctcaac agattccttt gcagagagca agcctcctgc cagtccattg
     1741 ggagctggct gtatagcata tgttatgttc attccaagat tggagccatc accaagaaga
     1801 ccttcaaact gagatttatc ttcaggatta ataacaataa ccagctctct tatctccata
     1861 agcatcatca aagacagtgg ataataaatc attggcttgt tatatactgg aaggagatgc
     1921 ttattcgtgc ttattgttac cggatacagc cttgtgcctg ttccacctgc caaaattaca
     1981 cccttcatgt tttcctcctg tctgctctat tatagcatag caaa
//
LOCUS       DAOTKU010000319         2016 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_45024_length_2016_cov_3.823559, whole genome shotgun sequence.
ACCESSION   DAOTKU010000319 DAOTKU010000000
VERSION     DAOTKU010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45024_length_2016_cov_3.823559"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="P5310_07175"
     CDS             complement(<1..445)
                     /locus_tag="P5310_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014650231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HRU33176.1"
                     /translation="MLYPKESETREVKNLSGVWRFKVDRENTGLEEAWYSKPLEDTIP
                     MPVPSSYNDVTQDISIRDHIGYVWYEREFFVPSSWKDKRIFIRIGSASHFSIVWINGE
                     KVLEHKGGFLPFEAEITQIVKFEGENRLTIAVSNILDWTTLPPGEM"
     gene            complement(731..>2016)
                     /locus_tag="P5310_07180"
     CDS             complement(731..>2016)
                     /locus_tag="P5310_07180"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRU33177.1"
                     /translation="SQFHPKYNSKMKDYETLRLKSQWWNNPDYPTLFAWRCAAYEAGK
                     RVVFERNHYYWKVDTAGNQLPYIDRIEAEYVVDPEVRLLKVLNGEYDATFRGIEDPRQ
                     IPVLMERAEKGGYRVILWKNGAGAWPGMIVNQTYNVEKTKDPWMRDLLRNRDFRAALS
                     LAIDRNRINNVIWKGIGKVQQGTISEESWHFQSAQGKKVFQEWANNLAKYNPEEANKL
                     LDKIGLTKRDSEGFRTRPDGKKLEIILDVTGWGTVPINTEAAPIIKKNWEDIGLRVIL
                     NDVYNTPEEGLRDNEGTYMIRMAHVAEMDLWTYPDWVFPGPNNRCWPLISLWIRTGGD
                     QGEKPEPGSPEDRLLKLYQKGIAEKDLVKRHKYVWDAVQIHIKEGPFFIGITGALPTP
                     VIVRKNFRNVPNFGVLGPWAIGGPGNTCPEQYFFE"
BASE COUNT          508 a          496 c          365 g          647 t
ORIGIN      
        1 tcatttcacc tggaggtaaa gtggtccagt ccaggatgtt actcacagca atggttaacc
       61 tgttctcccc ttcaaacttt actatctgag taatctcagc ctcaaaaggt aaaaatccac
      121 ccttatgctc caggaccttc tctccgttta tccagactat agagaaatgg ctggcacttc
      181 caattcttat aaagattctc ttatccttcc aggaggatgg gacaaaaaac tccctctcat
      241 accagacata acctatgtgg tctcttatag agatatcctg agttacatcg ttatagcttg
      301 aaggaacagg cataggaatc gtatcttcca gaggttttga ataccaggct tcttccagcc
      361 ctgtattctc cctatcaacc ttaaatctcc agactccgga tagatttttg acctctcttg
      421 tttcgctctc tttgggataa agcatccgtg acctcctctt ttctgtaatt atatatttaa
      481 gacttctgag tattatttta acatataacc aatagcctgt gtaagataaa aaccgtgatg
      541 cgtgatggga gagggtgaaa ggaagaagat gcccccacct caattctccc ccggggtggg
      601 ggaggaaaag ctaaaatagt tggtgaatag gatagcttat tcctagattt atattttcat
      661 acaggaaatc gcttctttga acaaagaagg aggccctctt aagagggcct ctcttttgcc
      721 accttacaga ctattcaaag aagtactgct cagggcaggt attaccgggt ccaccaattg
      781 cccagggtcc aagaacaccg aaattaggca catttctgaa gttttttctg actatcaccg
      841 gtgtaggtag cgcaccagtg atacctataa agaatggtcc ttctttaata tgaatttgaa
      901 cagcgtccca tacatattta tgtctcttta ccagatcttt ttctgctata cccttttgat
      961 atagtttgag cagacggtct tctgggcttc ccggttcggg cttctcacct tgatctccac
     1021 ctgttcttat ccagaggctt atcaatggcc agcatctatt attaggacca gggaaaaccc
     1081 agtccggata tgtccagagg tccatctcgg ctacatgagc catacgaatc atataagttc
     1141 cctcgttatc tctaagcccc tcctctgggg tattgtaaac atcattcaat attactctta
     1201 atccgatgtc ttcccagttc tttttaatta tgggagcagc ttctgtattt atagggacag
     1261 taccccaacc agtaacatct agtatgattt ctagtttctt accatctggt ctggttctga
     1321 agccttcaga atccctcttg gttaaaccta tcttatctag aagtttattc gcctcctcag
     1381 gattatattt agccaggtta ttagcccact cctgaaagac cttcttaccc tgtgcactct
     1441 ggaagtgcca ggattcctca cttattgtac cctgctgtac ttttccgatg cctttccata
     1501 tcacattatt aattctgttt ctatctatag ctagagaaag ggctgctcta aagtcacgat
     1561 ttctgagcag gtctctcatc catggatctt tcgtcttctc tacattatat gtctggttta
     1621 ctatcattcc tggccatgca ccggcaccat ttttccagag tataactctg taccctcctt
     1681 tttcagctct ttccatcaat actgggatct gtcttgggtc ctctattcct ctgaatgtag
     1741 catcatactc tccattcaat actttgagta atctcacctc tggatcaacc acatattctg
     1801 cctctatcct atctatatat ggaagctgat tcccggcggt atccaccttc cagtagtagt
     1861 gattacgctc aaagactacc ctctttccag cttcataggc tgcacatctc caggcaaaga
     1921 gggtaggata gtcagggttg ttccaccact gagacttcaa cctcaatgtt tcgtaatctt
     1981 tcatcttgga gttatacttt ggatggaatt gactca
//
LOCUS       DAOTKU010000320         2009 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_45239_length_2009_cov_3.000512, whole genome shotgun sequence.
ACCESSION   DAOTKU010000320 DAOTKU010000000
VERSION     DAOTKU010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45239_length_2009_cov_3.000512"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..931
                     /gene="ilvC"
                     /locus_tag="P5310_07185"
     CDS             <1..931
                     /gene="ilvC"
                     /locus_tag="P5310_07185"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HRU33178.1"
                     /translation="IGYGSQGRAQSRNLKDSGVKVLVGLREGGKSWNLAKSEGMDVVS
                     IEDAANAGDVIQILLPDQIQQEIYARSIEKYLKEGKALGFSHGFNIHYHQIVPPEGVD
                     VFMVAPKGPGPLVRRMYEEGKGVPSLIAIYRDYSGKAKDIALSYAKAIGSLRAGVIET
                     TFKEETETDLFGEQAVLCGGVNELIKYGFEVLVEAGYQPEIAYFECLNELKLIVDLIY
                     EGGIVNMWRGVSDTAKYGGLTVGPKIITEEVKELMKEALKKVQNGEFAREWVLENKSG
                     QPVLKASVEAAENHLIEKVGKELRDMMPWIKRG"
     gene            933..>2009
                     /locus_tag="P5310_07190"
     CDS             933..>2009
                     /locus_tag="P5310_07190"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013931814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HRU33179.1"
                     /translation="MRRIYIFDTTLRDGEQTPRVTLEPQEKLRIARQLEEMGVDVIEA
                     GFPIASPGDVEAVTIISKEIKNSTVCGLARSVKKDIDVAWSALQYARKPRIHVFIATS
                     EIHMKYKLKKTKEEVLEQAVESVRYARSFCDDVEFSAEDATRSDPEFLKEVFLGVIDA
                     GATVINVPDTVGYCIPNEFGNFIAQIKSYLPEDIILSIHCHNDLGLATANSLLGVLNG
                     ANQVEVSVNGLGERAGNAALEEVVMAIRTRKDAFDAYTNLNTEYIINLSRLVSGFTGI
                     PVQPNKAIVGINAFAHEAGIHQDGVLKERSTYEIMNPKDIGLDSSEIVLGKHSGRHAL
                     NERLKQLGYSLSEEELMKVFERFKV"
BASE COUNT          617 a          324 c          555 g          513 t
ORIGIN      
        1 tatagggtac ggaagtcagg gaagggcaca gtcaagaaat ctgaaggata gcggagtaaa
       61 ggttttggta ggcttgagag aagggggaaa atcatggaat ctggcgaagt cagagggtat
      121 ggatgttgta agcatagagg atgcggctaa tgcaggagat gttatacaga tcttgcttcc
      181 tgatcagata cagcaggaga tttatgccag gtctatagag aaatatctga aggaggggaa
      241 ggctctaggt ttctctcacg gatttaacat ccactaccat cagatagtac ctccagaagg
      301 tgtggatgtt tttatggtag ctccaaaagg tcctggacct ctggtaagaa ggatgtatga
      361 ggaagggaag ggtgtaccaa gccttatcgc catttacaga gattattctg gcaaggctaa
      421 agatatcgcc ctttcctatg caaaagcgat tggttcgttg agggctggtg tgatagagac
      481 cacatttaag gaagaaacag agacagatct attcggtgag caggctgttc tctgcggtgg
      541 ggtaaatgaa cttataaagt atggatttga ggttcttgtt gaggcaggat atcaacccga
      601 gatcgcatat tttgaatgtc ttaacgagtt aaaacttata gttgacctga tatatgaggg
      661 tggaattgta aatatgtgga gaggtgtaag tgatactgct aaatatggtg gactcacagt
      721 aggaccaaag attataactg aggaagtaaa ggagcttatg aaagaagcac ttaagaaggt
      781 ccagaatgga gagtttgcca gggaatgggt actggagaat aagtctggtc agcctgttct
      841 caaagccagt gtagaagcag ctgaaaatca tcttatagaa aaggttggta aggaattgag
      901 ggatatgatg ccctggataa agaggggata gtatgagaag aatttatatc tttgatacta
      961 ctctcaggga tggcgagcag acccccaggg ttactcttga gcctcaggaa aaactgagaa
     1021 tagccagaca gctcgaagag atgggggtag atgttataga ggctggtttt ccaatagcct
     1081 ctcctggtga tgtcgaagcg gttactatca tatcaaaaga gataaagaac agtacagtgt
     1141 gcggacttgc caggtctgta aagaaggata tagacgttgc atggtctgcc ctgcagtacg
     1201 ccagaaagcc aaggatacat gtctttatag ctaccagcga gatacatatg aagtacaagc
     1261 ttaaaaagac aaaagaagag gtactagagc aggcggtgga atcggtcaga tatgcgaggt
     1321 ctttctgtga tgatgtggag ttttcagctg aagacgctac caggtctgat ccggaattct
     1381 taaaagaggt atttttaggg gttatagatg ctggggcaac cgtcataaat gttccagata
     1441 ctgtaggtta ctgtataccg aatgaatttg gtaattttat agcccagata aagagttatt
     1501 tgcctgagga tataatctta agtattcact gtcacaacga cctgggactt gccaccgcca
     1561 attctctgct aggtgtattg aatggtgcaa accaagtaga ggtttccgtg aacggacttg
     1621 gagagagggc gggaaacgca gcccttgaag aggttgttat ggctataaga acaagaaaag
     1681 atgccttcga tgcctatacc aaccttaata ccgagtatat tatcaatctg agcagactgg
     1741 tgagtggctt taccggtata cctgtccagc ccaataaggc tatagtgggg attaatgcct
     1801 ttgctcatga ggctggaatc caccaggatg gggttctgaa agagagaagc acttatgaga
     1861 taatgaaccc caaagatatt ggacttgact catccgagat agtcctgggt aaacattctg
     1921 gaagacatgc cctcaatgag aggttgaaac agctgggtta ttcgctatca gaggaagaat
     1981 tgatgaaggt ttttgaaaga tttaaggtt
//
LOCUS       DAOTKU010000321         1995 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_45655_length_1995_cov_2.648969, whole genome shotgun sequence.
ACCESSION   DAOTKU010000321 DAOTKU010000000
VERSION     DAOTKU010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45655_length_1995_cov_2.648969"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1707)
                     /locus_tag="P5310_07195"
     CDS             complement(<1..1707)
                     /locus_tag="P5310_07195"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HRU33180.1"
                     /translation="MSPSLEKWIEECASLTKPDQIYWCDGSEEEAHRLIAIGMYKEKI
                     NDNPVFSELNHKLWPSSYLHRSHPTDVARTEHLTFVCHSDKENAGPNNNWMDPREAKE
                     LLRKLSDGCMKGRTMYVLPYIMGHPDSPYSRVCVQVTDNSYVAASMRIMTRMGKIALE
                     KLGDGGDFVRGIHSVGDFDPNKRFIMHFPDENLVWSIGSGYGGNALLGKKCFSLRIAS
                     WLGLKEDWLAEHMIIIGVEDPDGNITYIAAAFPSACGKTNLAMLRSTLPGYKVWTIGD
                     DIAWLNIGPDGRLYAINPETGFFGVAPGTSMKTNPNMMETLKAGTFYPTLFTNTALNV
                     DTNEPWWEGMDGPVPENILDWQGRKWDKTGGNTAAHPNSRFTVSISQCPTLSPEFDNP
                     QGVPISAIIFGSRRSRLLPLVYESFSWSHGVFCGARMGSETTAAAIHQTGKLRRDPMA
                     MIPFCGYNMGDYFAHWLNMGRKLSNPPKIFGVNWFRLDEDGRFLWPGFGENMRVLKWI
                     IDRINNNVKAKESPIGLLPYIEDLELSGLNISRENMEKLFKIDNDEWKLELEDVRSFF
                     NQF"
BASE COUNT          545 a          454 c          371 g          625 t
ORIGIN      
        1 aaattgatta aagaatgacc tgacatcttc gagctccagc ttccattcgt cattatcaat
       61 cttgaataat ttttccatat tctccctgct tatatttaga ccgctgagtt caaggtcctc
      121 tatataagga agaagtccaa ttggggattc ttttgcctta acattattgt ttatcctgtc
      181 tattatccac ttcagaaccc tcatattttc tccaaaacct ggccagagaa atctgccgtc
      241 ttcatcaagc ctgaaccagt ttacgccaaa aatcttagga ggattactga gctttctacc
      301 catatttaac cagtgtgcaa aataatctcc catattatag ccacagaaag gtatcatagc
      361 cattgggtct ctacgtaatt ttcctgtctg gtgtatggcg gcagctgttg tctccgagcc
      421 catcctggct ccgcagaata ccccatgaga ccagctaaaa ctctcataaa caagcggtaa
      481 aagcctgctc cttctgctcc caaatattat tgcagatatg gggactccct gaggattatc
      541 aaactcaggg gaaagtgttg gacactgact tattgaaaca gtaaatctgg aatttggatg
      601 ggcagcggta tttccacctg ttttatccca ctttctaccc tgccagtcca gtatgttttc
      661 cgggacaggt ccgtccatac cttcccacca gggttcgttg gtatcaacat taagagctgt
      721 attggtaaaa agtgtgggat agaatgtccc tgccttcaga gtctccatca tatttggatt
      781 ggttttcata gatgtaccgg gagcaactcc aaaaaagcca gtttcaggat ttatagcgta
      841 taatctgcca tccggaccta tgttcagcca ggcaatatca tctcctattg tccatacctt
      901 atagcccgga agggttgatc tgagcatagc caggttggtt ttaccgcatg cagatggaaa
      961 tgcagctgct atatatgtta tattgccatc cgggtcttct accccgataa taatcatatg
     1021 ctccgccaac cagtcttctt tcaatcccag ccatgatgcg attcttaaag agaaacattt
     1081 tttgccaaga agggcattcc ccccataccc ggacccgata ctccagacca gattttcatc
     1141 aggaaagtgc ataatgaatc ttttgttcgg gtcaaagtct ccgaccgaat gaattcccct
     1201 tacaaaatct ccaccatcac cgagtttttc cagtgctatc ttgcccatcc tggtcattat
     1261 cctcatactt gccgctacat aggagttatc agtgacctga acgcataccc tggaatatgg
     1321 agaatcagga tgtcccatta tatatggcaa tacatacatg gtcctccctt tcatacaccc
     1381 gtcacttaat ttcctaagta actctttagc ttctctgggg tccatccagt tattattagg
     1441 accggcattc tctttgtcag aatgacagac aaatgtaaga tgttctgtcc ttgcaacatc
     1501 tgtaggatga ctcctgtgta agtaagagga gggccataac ttatggttca actcagagaa
     1561 tacaggatta tcatttatct tctctttata cattcctatc gctatcaacc tgtgggcttc
     1621 ttcttctgat ccatcacacc agtatatctg gtctggttta gttaagctgg cacattcctc
     1681 tatccatttc tcaagagagg gtgacattgt ttacaacctc cttcttagaa gattactcaa
     1741 ttttatatat ggtataaaag attaccagct tctggatgaa tgtcaagtgt ttttattctg
     1801 aaattgtatg gaccggtaat ttttgtatgg aattttctaa tctgatggtg tctctaatag
     1861 cctcaaaata actacggttg atattttaat atagaagggt atactctata agtagactat
     1921 ttagaaaggc ttaacagttt aactggagga ggattaattg tgtcagatat aaaaaacagg
     1981 atatttttag agcag
//
LOCUS       DAOTKU010000322         1984 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_46027_length_1984_cov_2.722136, whole genome shotgun sequence.
ACCESSION   DAOTKU010000322 DAOTKU010000000
VERSION     DAOTKU010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46027_length_1984_cov_2.722136"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1937)
                     /locus_tag="P5310_07200"
                     /pseudo
     CDS             complement(1..1937)
                     /locus_tag="P5310_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012398983.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
BASE COUNT          500 a          528 c          300 g          656 t
ORIGIN      
        1 cactctccct tgggcatcag taagacgtcc atcatccagt atctgcaata atatgttaaa
       61 cacatcagga tgtgcctttt ctatctcatc aaagagtaca atcgaaaatg gtctgcgcct
      121 tacagcttca gttagctgtc ctccttcttc ataacctaca tatccgggag gagatcctac
      181 aagtctggaa actgcaaact tttccatata ctcagacata tctatcttga taagtgcctc
      241 ttcgtcgttg aatagaaatg aagctaaggc tctggccaat tctgtctttc caactccagt
      301 aggaccgagg aagataaatg aaccaatagg ttttctgggg tcttttatac cagctctggc
      361 tcttctaatt gcctcggata caacatttac agcctcttcc tgaccaacta ttcgcttgtg
      421 taactcttct tccatatgta tcagtttttc cacttctttt tccaaaagct tagtaactgg
      481 gataccggtc cacagagata acacttctcc tatatcttca tccgttacta cgggttcttt
      541 tctattcacg ttcagtacat catcagttac cgactggatc tgatcgataa gaaacttctc
      601 tctgtccctt aactgagctg ctctctcgta atcttgagac tttactgcca gttccttctc
      661 ccctataatc ctctccagtt ctatctccag atccttcctg gaactgggca tctctgatag
      721 ctccaatctt actttagccg aagcctcgtc aatcagatct atagccttat caggtagatg
      781 tctatcagat atatatctat ccgagaggat ggcagccgct tctatagcct tatctgttat
      841 cttcacctga tgatgagtct catacctctc ctttatacca ttgagtatca taatggtatc
      901 ttcaacagat ggttcctgca ctataattgg ctgaaatctc ctctctaaag cagaatcttt
      961 ttctatgtat tctctatact catctaatgt ggtagctccg atacactgta attcgcctct
     1021 ggagagagaa ggtttcaaga tattagaggc atccatcgcc ccttcagcag ctcctgcccc
     1081 taccatagta tgcaattcgt caacaaagag tactatctct ccgttagctc tacgtatctc
     1141 atcaagaata cgtttcatcc tctcttcaaa ttctccccta tactttgttc cagccacgat
     1201 agccgccaga tctaatgcta ttattctctt attatacaaa atgggaggac cttctctttt
     1261 tactatacgc tgagcaagtc cttccactat tgctgtctta cccactccag cttcacctat
     1321 gagaaccgga ttattctttg tcctcctgca gagaatctgc atcaatctac gtatctcttt
     1381 gctcctccct ataacaggat caagttttcc ttctctggct agctgagtaa ggtctctacc
     1441 aaactgattt agtagaggcg tctttgctct ggatttactg gtactttctg ccggtacagt
     1501 cttcccttct ccaaggaaat atagaacctc acttctagct ttatctaaag tgactccaag
     1561 actctctaaa atcttagctg caactccctc tccttctctt atcaatccaa gaagtatatg
     1621 ttctgttcct atatagttct gtcccattaa ttttgattct tcataagcca actctaaagc
     1681 acgtttagcc cttggagtaa gcgtcatctc cgggagatat attcctcccc ctctacctat
     1741 gagctcctcc accttagccc ttacatagcc cagatctata accagctgct taagacctta
     1801 gctgctatac cctctccttc agctaaaagc ccaaggagta aatgttccgt ccctaaataa
     1861 ttatgattaa gccttctagc ctcttctctt cctaaagtta agactcttct cgcttgatca
     1921 gtaaatttgt cccacatttt aaactcctta agattgcttc tttataaaga ttatactata
     1981 tata
//
LOCUS       DAOTKU010000323         1983 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_46057_length_1983_cov_3.301349, whole genome shotgun sequence.
ACCESSION   DAOTKU010000323 DAOTKU010000000
VERSION     DAOTKU010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46057_length_1983_cov_3.301349"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            307..1413
                     /locus_tag="P5310_07205"
     CDS             307..1413
                     /locus_tag="P5310_07205"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRU33181.1"
                     /translation="MDKNLIINAHVHFENISLIRETKEFFQGLAIEKLNIVSPARPDR
                     VNSNPQAICFKAMYPGDIYISGGLDYSGIEGKSDREIEKLFKDQVHRLKEIGFDGIKL
                     LETKPNYARIMPFPIDSPLYNSFFSTIEELDLPIFWHVADPEEFWDKERIPPVAKERG
                     WDYTDGSYPSKEELYQRVDNVLKRFPGLKIVFAHFYFLSADLKRAENLLDRFPNINLD
                     ITPGSEMYYNFSKDPEKTREFFIKYQDRIVFGDDTSVSRNGIMKELITNRIKFMRDFL
                     ETDEEFSIGPTDKNFLARPDRVKGIKLPQSVLEKIYRLNFLRIAGENPLPLNISLAKE
                     ECRRIGEVLEKRYNYPEDENFGLQAVDFLREMFE"
     gene            1462..>1983
                     /locus_tag="P5310_07210"
     CDS             1462..>1983
                     /locus_tag="P5310_07210"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HRU33182.1"
                     /translation="MDRIIRAYSRLIESNLDRPELVKSLIRFGLEVGKVWVDHFNRNG
                     IPPSLRYLNSICFQFILEPLRHPENSGFVNLLAPAEILHSMDIYPMFIEAYSSFVSGF
                     YCEDIFIDRAESRGLSGTLCSYHKTFLGSLEYSVLPRLKFAVTSSILCDGNSNTFKYI
                     SKSYKIPYYIVDVP"
BASE COUNT          622 a          330 c          475 g          556 t
ORIGIN      
        1 atactgagtc aaggctgacg ggagacataa atgccgtgac gcgtgacggg tgaagcgtaa
       61 tgaatgggat ttcctcttcc ctgacgagaa ttttcctttc tccctctccc ttgacgggag
      121 agggcagggg tgagggtgaa agggtatagg tttattatta taaaagacga aattgtaggg
      181 gcaattagtg acaggtgagg gcacgattta tctagcaaaa tgatgtggga ctggataaat
      241 ctatgttaat tttgccagaa tctgatagaa tattatagaa tatgctggca ggagggaaaa
      301 tatcagatgg ataaaaactt aattattaat gcccatgtcc attttgagaa catatcttta
      361 ataagggaga ccaaagagtt ctttcagggg ttggcgattg aaaagttgaa tatagtcagt
      421 ccggcacgac cagatagggt aaacagtaat ccccaggcta tctgtttcaa ggctatgtat
      481 ccgggagata tatacatctc tggcggcttg gattactccg gtatagaggg gaaatcggat
      541 agggagatag aaaagttgtt caaagatcag gtccacaggt taaaggaaat cggatttgat
      601 gggataaaac ttctggagac aaagcctaat tatgctagaa ttatgccttt tcctatagat
      661 agccctttat ataactcttt tttctcaacc atagaggaat tggatctacc tatcttctgg
      721 catgtagccg atcctgagga gttctgggat aaagagagaa tcccaccggt agcgaaggag
      781 agaggatggg attataccga tggtagctat ccgtcaaagg aggaactgta ccagagggta
      841 gataatgtac tgaagagatt tccaggattg aaaatcgtct ttgcacattt ttatttcctc
      901 tcggcagacc tgaagagggc tgaaaatcta ttggatagat ttcccaacat caacctggat
      961 attacacccg gttctgagat gtactataat ttttcgaaag accctgaaaa aacaagagaa
     1021 ttctttataa aatatcagga caggatagta tttggtgatg acacctcagt atctagaaac
     1081 ggaataatga aggaactgat aactaacagg ataaaattta tgagagactt cctcgagaca
     1141 gatgaagagt tttcgattgg accaaccgat aagaatttcc ttgccagacc agacagggtg
     1201 aaggggataa aattaccgca gtcagtgctg gaaaagatat acaggctgaa ttttttaagg
     1261 atagctggag agaatcctct gcctctgaat atctccctgg caaaagaaga gtgtcggagg
     1321 ataggagaag ttctggaaaa gagatataat tatcctgaag atgaaaattt cggtcttcag
     1381 gcggtagact ttcttagaga aatgtttgaa tagatggcgg tgtcttcttt ctgagatact
     1441 gggcagagcc ctgatatatt tatggataga attataaggg catacagtag actgatagag
     1501 agtaatctcg atagaccaga actggttaag agtctgataa gatttggtct tgaggttgga
     1561 aaggtatggg ttgaccactt taacaggaat ggcatacctc cttctctcag atatttaaac
     1621 agtatatgtt ttcagtttat tctggagccc ctgagacatc ctgagaactc tggatttgta
     1681 aatcttcttg caccggcaga gatactgcac tctatggaca tatatccaat gttcatagag
     1741 gcatactcat catttgtctc tggattttac tgcgaggata tatttataga cagggctgag
     1801 tcgagaggtc tctcggggac attatgcagt taccataaga catttcttgg ttcacttgaa
     1861 tactcagtac tgccgaggct gaaatttgca gtaacatctt ccatactgtg tgatggaaat
     1921 tccaatacat tcaaatatat atcaaagagt tataaaattc catactacat agtagatgta
     1981 ccg
//
LOCUS       DAOTKU010000324         1982 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_46083_length_1982_cov_4.847950, whole genome shotgun sequence.
ACCESSION   DAOTKU010000324 DAOTKU010000000
VERSION     DAOTKU010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46083_length_1982_cov_4.847950"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..876)
                     /locus_tag="P5310_07215"
     CDS             complement(<1..876)
                     /locus_tag="P5310_07215"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRU33183.1"
                     /translation="MERITFPPRIIFDEREKDAILRVVEKTMTGPEAIDMYGKGPEVA
                     SYEKEFAEYFGVKYAAAVSSGTAAVHSAVGALRLEPGSEIITTPITDPGTVAPILMQN
                     CIPIFADVEYDTLNISPKSIEKNITDKTRAIIAVHLAGQPCDMDPIMEIARRHNLYVI
                     EDCAQAHGARYKGRYVGTMGDLGVFSLMAGKHMNSGGQGGMVITNNEELYWNAKRFSD
                     RGKPFNSEEKTNLLLGLNYRMTELQAAVGRVQLTKLADIKGKRRWVVERLREGTSGLK
                     MFQLWRFIDNVEVNPW"
     gene            complement(879..>1982)
                     /locus_tag="P5310_07220"
     CDS             complement(879..>1982)
                     /locus_tag="P5310_07220"
                     /inference="COORDINATES: protein motif:HMM:NF020121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase trimerization
                     domain-containing protein"
                     /protein_id="HRU33184.1"
                     /translation="EPVTDTALLFSSQTSRYYISSLGELYRDLGSGKEQDLIIDMGSG
                     KSIIDWGKRKQVCENLMAYAYEGYFTALTRNHIMFDIVLDEDLNLEKLSKYKVLILPD
                     FACLSDIQWRSIETFVREGGILIASFEAGLYDEKGNPKFTEERLNLFGIREIEEAFPP
                     VRGENYIIATEDFFGFNKNNLIERSPYALKVKIKENIKAPFFFMNPLEGVYMPIKGIS
                     DYPAVIINNSGKGRVIYFAFPLGSFYGSERIPTQEQLIKYAVENLKTSEIEVSAPPTV
                     DVETYRQRGTGRLIIQLINNTGDMQRPLTYINPVSDIEIAVRDSSVESGYTLSDKNPV
                     SLYKKDGKVSFKIELLKDFEVIVLDSNIENRRS"
BASE COUNT          548 a          493 c          291 g          650 t
ORIGIN      
        1 ccagggattt acttctacat tatctataaa tctccatagc tgaaacatct taagaccaga
       61 cgtcccctct ctcaatctct caaccaccca tcgcctctta ccctttatat cagcaagctt
      121 ggtaagctgg actcttccta ctgctgcctg aagttcagtc attctgtaat ttagccccag
      181 taagagatta gtcttctctt ccgaattaaa tggtttccct ctatcagaga atctctttgc
      241 attccagtac aattcttcat tattagttat aaccattcca ccctgtccac ctgaattcat
      301 atgtttccct gccataagac tgaacacccc aagatccccc attgttccaa catacctgcc
      361 cttatatctt gcaccatgag cctgagcaca atcttctatt acgtatagat tatgcctcct
      421 ggctatctcc attatagggt ccatatcgca gggttgtcca gccagatgaa cagctattat
      481 cgccctggtc ttatcagtga tattcttttc tatggatttt ggagatatat tgagtgtatc
      541 atattccacg tctgcaaata ttggtataca attctgcata agtattggag caactgtccc
      601 cggatctgtt atcggtgttg taataatctc acttccaggt tcaagcctta aagctcctac
      661 agcagaatgc actgcagccg ttcccgaact caccgcagcc gcatatttta ccccaaagta
      721 ctcggcaaat tccttctcat aagaggcaac ctctggtcct ttcccgtaca tatcgatagc
      781 ttctggaccg gtcatcgtct tttctacaac cctaagaata gcatcttttt ctctttcatc
      841 aaaaataatc cttggaggaa aagtaattct ttccataatc aagacctcct attttctata
      901 ttagaatcca atactataac ctcaaaatct tttaacaatt ctatcttaaa agatacttta
      961 ccatccttct tgtaaagaga aactggattt ttatcactca gtgtataccc gctttctact
     1021 gagctatctc tcactgcaat ctctatatca ctgacaggat ttatataagt caaaggtctc
     1081 tgcatatcac cggtattatt tatcagttgt attataagac gacctgtccc cctctgtcta
     1141 taggtttcaa catccacagt cgggggagca ctcacttcaa tctctgaggt cttcaagttt
     1201 tccacagcgt actttataag ctgctcctgg gttggtatcc tctctgaccc atagaatgaa
     1261 ccaagaggaa atgcaaaata tatcaccctg cccttaccag aattatttat tataacagca
     1321 ggataatcgc ttataccttt tattggcata tagacccctt ctagcggatt cataaagaag
     1381 aatggagctt ttatattttc ctttatctta accttcaagg cgtatggaga cctctctata
     1441 agattattct tattaaatcc aaagaaatct tcggtagcga ttatataatt ttctcctcta
     1501 accggaggga aagcctcctc tatctctctt ataccaaaaa gatttaatct ctcttcagta
     1561 aattttggat ttcccttctc atcatataga cctgcttcaa atgaagctat cagtatccct
     1621 ccttctctta caaatgtctc tatactcctc cactgtatat cgctgagaca ggcaaaatca
     1681 ggaaggatta agactttata tttcgataat ttttctagat tcaaatcttc gtctaataca
     1741 atgtcaaaca ttatatggtt tcttgtcagt gccgtaaaat atccttcata tgcatatgcc
     1801 ataagattct cacatacctg ttttctctta ccccagtcta taatagattt acctgacccc
     1861 atatcaatta tcaagtcctg ctctttaccg ctacccaaat ctctgtaaag ttctcccaga
     1921 ctagatatat agtatcgaga cgtctgggaa gaaaacagga gggcggtgtc ggttaccggt
     1981 tc
//
LOCUS       DAOTKU010000325         1981 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_46125_length_1981_cov_3.195742, whole genome shotgun sequence.
ACCESSION   DAOTKU010000325 DAOTKU010000000
VERSION     DAOTKU010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46125_length_1981_cov_3.195742"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /gene="ybeY"
                     /locus_tag="P5310_07225"
     CDS             <1..302
                     /gene="ybeY"
                     /locus_tag="P5310_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020153989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HRU33185.1"
                     /translation="KEINVVLTDDEYIRRLNKDYRGIDSPTDVLSFSMDEDKVWGDIY
                     ISLDTARKQAEELGWDLDREIRFLAIHGMLHLIGYNDEDEEGYKKMMELTDELLG"
     gene            305..1006
                     /locus_tag="P5310_07230"
     CDS             305..1006
                     /locus_tag="P5310_07230"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF013391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - diacylglycerol kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HRU33186.1"
                     /translation="MSKTRPLHESIDLAVDGIISALREERNFRIEIISAGAVVACGII
                     LNLTRIELAILSLVIAVVLSAELFNTAIEKLLDMVNNNYDPKIKFIKDVSAGAVLLTA
                     VFSVVVGYLIFYPHLETPFKNSIRLLRAIPYHLLVGGFILIILMVALLKGLYGDRFSK
                     GGVVSGHSAVAFGLAIAILYLSGSLLISLLGFVLAFMVAQSRVEGNIHSWREVILGSF
                     LGVIVMLTIFEFLLR"
     gene            1093..>1981
                     /locus_tag="P5310_07235"
     CDS             1093..>1981
                     /locus_tag="P5310_07235"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HRU33187.1"
                     /translation="MSSETAITAVSKVKLKQLENEGDVRAKRLNNLLEDSTRLLSTIL
                     FGNNIAQNALAALVTTASFRWLNRAGLSAGWAIPLSTIVTTFLILVFAEVTPKSYAFQ
                     RAEKIALFTAVPMSVIHRLFSPFAIGITAIANSILRLFGLSLVNPEPFVIEDEIKTLV
                     DLGEEEGVIEEEEKEIIEGVFEFSETLAREVMVPRVDIVALDVNSSFDEAIDTINMSG
                     HSRIPVYDETIDNIVGIIYAKDLLKFFGSKNPPPLRSIMRAPYYVPETKPIDELFREM
                     RAQKVHMAIIIDEYGGTAGL"
BASE COUNT          559 a          312 c          484 g          626 t
ORIGIN      
        1 ttaaagagat aaatgtggta ctgacagatg atgaatatat tagaaggctc aataaagact
       61 atagagggat agattctcca accgatgtac tcagcttttc tatggatgaa gataaggtat
      121 ggggagatat atatatctct ctggatactg caaggaagca ggcagaggaa ctggggtggg
      181 atctggatag agagattaga tttctggcta tacatggaat gcttcacctg ataggctata
      241 atgatgagga tgaggagggg tataaaaaga tgatggaact tacagatgag ttattaggat
      301 agatatgtct aagacgagac ctctgcatga gagtatagat ctggctgtag atggaataat
      361 atctgctcta agggaagaga gaaactttag gatagagatt atctctgctg gagcagttgt
      421 agcttgtggt ataattctga atcttacaag gatagagctg gctatcctct ctcttgtcat
      481 cgctgtggtg ctttctgcag agttgttcaa taccgctata gaaaagttgc tcgatatggt
      541 aaataacaat tatgatccaa agataaagtt cataaaggat gtgtcagcag gagcagtcct
      601 cctcactgca gtgttttctg ttgttgttgg ctatcttatt ttttatccac atctggaaac
      661 tccatttaaa aattcaataa gattactgag agcaattcca tatcacctcc ttgttggtgg
      721 attcatcttg attatactta tggttgcgct gttgaagggg ttatacggtg atagattctc
      781 caaagggggt gttgttagtg ggcattcagc ggtggcattc ggtctggcta tagctatact
      841 ttatctttct ggttctttgc ttatatcttt attgggattt gttcttgcat ttatggtggc
      901 tcagagcagg gtggagggga acattcattc atggagagag gtaatccttg gttctttcct
      961 tggtgtaata gtgatgttaa caatatttga atttcttctt agataggagg gaaactttga
     1021 ttggaagacg gtggtagttt ttggttcaac tatgttctga tgtttttctt tcttttctta
     1081 tcagcgtact ttatgagttc agaaacagct ataacggcag taagcaaggt taagcttaag
     1141 caattagaga atgaggggga tgtaagggct aagaggttaa ataatcttct ggaagattct
     1201 actagactcc tttccaccat actttttggt aacaatatcg ctcaaaatgc tctggctgcg
     1261 ctggtaacta cggcatcatt tagatggctt aatagagctg gattaagcgc aggttgggct
     1321 atcccgctat ctaccattgt taccaccttt cttattcttg tatttgccga agtaactcca
     1381 aaatcgtacg cctttcagag ggcggaaaaa attgccctat ttaccgctgt ccctatgtcg
     1441 gtaattcaca gattgttttc tccttttgct ataggtataa ccgctatcgc taattctatc
     1501 ttgaggcttt ttggactcag tctggtgaat ccagagccat tcgttatcga agatgagata
     1561 aagaccctgg tggatttagg tgaagaagag ggagtaatag aggaagagga gaaagagatt
     1621 atagaggggg tatttgagtt ttctgagaca ttggctagag aggtgatggt tcctagggta
     1681 gatatagtag ccctagatgt caattcgtcc tttgatgaag ctatagatac tataaatatg
     1741 agtggacatt ccagaatccc ggtttatgat gagactatcg ataatatagt ggggattata
     1801 tacgccaaag atcttctaaa attctttgga tcaaaaaatc ccccgccgtt aaggtctatt
     1861 atgagagcac cctactatgt ccctgagacg aaacccatag atgaactttt tagagagatg
     1921 agggcacaga aagttcatat ggctattata atcgatgagt atggagggac agctggattg
     1981 g
//
LOCUS       DAOTKU010000326         1968 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_46536_length_1968_cov_3.417669, whole genome shotgun sequence.
ACCESSION   DAOTKU010000326 DAOTKU010000000
VERSION     DAOTKU010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46536_length_1968_cov_3.417669"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(45..794)
                     /locus_tag="P5310_07240"
     CDS             complement(45..794)
                     /locus_tag="P5310_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU33188.1"
                     /translation="MRFALDLHIHTLLSPCAGIEMIPVNIVRRVQEVGIDIMAVTDHN
                     AVGNAESVIKSALKYAPGLGVFPGIEVTSKEEVHILGILPSVESARKLESMIHSHLVN
                     PPVKDSLAEQLIVDEDANFIRYEEHFLMGATDFSVEEVSDLIHSFDGIVIPAHIDRES
                     FGLIYNLGLFPPDLEVDAVEVSKFAKESTLVNLRRLIPPDVPVLFSSDAHFLDDIVPP
                     RSFLELMDKSFDSLKEALKNYGSVRNHKTGG"
     gene            complement(791..1126)
                     /locus_tag="P5310_07245"
     CDS             complement(791..1126)
                     /locus_tag="P5310_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HRU33189.1"
                     /translation="MTFRELLGNLPLENLGDDFDLDKSPRGGYVGDLLSNVMRFAKEG
                     NVWITVQTHINVIAVATLVGISGIIFPCGVEPPEETIKKAREEHIALLTTKLDAFTLC
                     GKIYELGIR"
     gene            complement(1130..>1968)
                     /locus_tag="P5310_07250"
     CDS             complement(1130..>1968)
                     /locus_tag="P5310_07250"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HRU33190.1"
                     /translation="ISFPSLVECLTPIATPEEISARYIKEELISEGYREDEVGVFLIT
                     PCTAKSADIKEGRGSHYINGTISVSEAYKYIIKSLKTVKEDRSGPIFGEGISWVRDGG
                     EAKSLGILNYLVVDGVKNVLKVLDDLELGKLRGINFIEGRMCGGGCVGGPLNVQNPYL
                     ARVKIRMLEDTYKESIDKSLYEYLEANDYFAGEEIRVDSSPYKLGEDVSSAIERMQEL
                     EEILKTLPGIDCGSCGAPTCRALAEDIVSGDARITDCIFKLKDNIEEYARSLLKMIRE
                     VK"
BASE COUNT          567 a          485 c          307 g          609 t
ORIGIN      
        1 ggatattatt tcatttaact gccttatctg attcgaatcc atctttaacc tcctgtttta
       61 tgattcctga cagatccata attttttaat gcttctttta atgaatcaaa actcttatcc
      121 atcaattcta agaaacttct gggaggaact atatcatcca ggaaatgggc atcactggaa
      181 aataataccg gaacatccgg agggataaga cgtctaaggt ttactaaagt gctttcttta
      241 gcaaatttgg aaacctctac tgcatctacc tcaagatcgg gaggaaataa cccaagatta
      301 tatataagac caaaggactc tctgtcaatg tgagcaggta taactatccc atcaaaagag
      361 tgaatcagat ctgatacttc ttctacagaa aagtctgtcg cccccataag aaaatgttct
      421 tcatatctaa taaagtttgc atcttcatca actatcaatt gttcagcgag agaatctttt
      481 accggaggat taaccaggtg ggagtgtatc atagattcaa gtttcctggc agattccacc
      541 gagggtagaa tccccaatat atgaacctct tctttagagg tgacctcaat ccctggaaag
      601 accccaagac ccggtgcata ctttaatgcg gactttatca cactttcagc attacccact
      661 gcattatggt cagtaacagc cattatatca attcctactt cctgcactct cctcactata
      721 tttacaggta tcatctctat ccccgcacag ggagataaaa gagtatgtat atggaggtcc
      781 aaagcaaatc tcatctaata cctaactcat atattttacc acagagtgta aaggcatcta
      841 actttgtggt aaggagagct atatgttcct ccctggcttt ctttatagtc tcttctggag
      901 gttctactcc acaggggaat attatcccac ttatgccaac cagagtagca accgctataa
      961 catttatatg agtttgaaca gtgatccaga cattgccctc tttggcaaat ctcattacgt
     1021 tactgagcag gtctcccaca tacccacccc tgggggactt atccagatca aaatcatccc
     1081 ccaggttttc taatggcaaa ttcccaagta attctctaaa cgtcatatat tattttacct
     1141 ctctaatcat ttttaataaa cttctggcat actcttcaat attgtcttta agcttaaaga
     1201 tacagtctgt tattctggca tctccactta ctatatcctc tgccaaagcc ctgcaggttg
     1261 gagctccaca ggagccacaa tcgataccgg gtaaagtttt tagtatctcc tccagttcct
     1321 gcatcctctc tatagcactg ctgacgtctt cacctaattt gtatggagaa gaatcaaccc
     1381 tgatttcctc tccagcaaag tagtcattag cttccagata ttcatataga gatttatcta
     1441 tactctcctt gtaggtatcc tctaacatcc ttatctttac cctggcaaga tacgggttct
     1501 gaacatttaa aggaccacca acacaacctc ccccacacat tcttccctca ataaaattta
     1561 ttcctctcag tttacccagc tccaggtcat ccaaaacttt cagtacattc ttcaccccat
     1621 cgacaaccag gtaatttaat atgccaaggc tcttagcctc tcctccatct ctcacccagc
     1681 ttattccttc cccaaagata ggaccactac gatcctcttt tactgtctta agagacttta
     1741 ttatatattt gtaagcctca gagactgata tggtaccatt tatataatga gagcctctcc
     1801 cctcttttat atctgcactt ttggcagtac agggagtaat aaggaatact ccaacctcat
     1861 cttccctgta accttcagat ataagctctt cttttatata tctggctgat atctcttcag
     1921 gtgtggctat aggggtaaga cactctacca gagaagggaa actgatct
//
LOCUS       DAOTKU010000327         1960 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_46786_length_1960_cov_3.058793, whole genome shotgun sequence.
ACCESSION   DAOTKU010000327 DAOTKU010000000
VERSION     DAOTKU010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46786_length_1960_cov_3.058793"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..110
                     /locus_tag="P5310_07255"
     CDS             <1..110
                     /locus_tag="P5310_07255"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF013421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HRU33191.1"
                     /translation="FLGHVIDYIQILNSPIFNLSDIAIIIGMIMILFSS"
     gene            125..865
                     /gene="lgt"
                     /locus_tag="P5310_07260"
     CDS             125..865
                     /gene="lgt"
                     /locus_tag="P5310_07260"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HRU33192.1"
                     /translation="MYPVILRLGNFSIRSWGLMVMTGIILAVLFAMREASKRGVDPEN
                     ILDLSIILIISGIIGARVLYVLVNFNSYRDNLGLAFSIKTGGLAWPGAFLFALIATIF
                     FIRWRKLSFWLIVDILAPYIALGYSVGRIGCFLNGCCFGKICEPAWWSVYMLGAYRYP
                     TQLVSSLTSFIIFLFLVTRDWYDKPGKRFLVYIILYCVYRFIIEFYRYNSTYIGGLSI
                     TQWGMLGTLIVTSLVLYGKSLEWKRRAT"
     gene            828..1718
                     /locus_tag="P5310_07265"
     CDS             828..1718
                     /locus_tag="P5310_07265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRU33193.1"
                     /translation="MERVWSGKEERLDLVVKEMLTDLSREKIKGIIEAGKVSVDGRVI
                     CKPSFKVKDGSDIRIEELPVEDNTLLPYDLPVDIVYEDESIAVVNKPPGLVVHPAPSV
                     KSPTLVNALVGKIELAPVNKERPGIVHRLDRDTSGLMVIAKTLQSYNALIREMQERRI
                     KKEYIAVVYGKVKSGMINAPIGRSQLNRTKMAVSPSGRIAITHYELLEIIGDYSLIRV
                     RIETGRTHQIRVHLSYIGHPVVGDRVYGRKEDETLISRQALHSSRLCFLHPVSGKLLD
                     FYSPIPEDIENLLNRLRGMI"
     gene            1715..>1960
                     /locus_tag="P5310_07270"
     CDS             1715..>1960
                     /locus_tag="P5310_07270"
                     /inference="COORDINATES: protein motif:HMM:NF022450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2837 family protein"
                     /protein_id="HRU33194.1"
                     /translation="MNRLLAVCILIGIIHMTDTLSLSVRWVGLKVGRVVTASSLFNIL
                     VIVSRTANLLQAPMVGKMVDLAIRNHQSLPLLNEFRLI"
BASE COUNT          554 a          312 c          468 g          626 t
ORIGIN      
        1 tatttctagg gcatgttata gattacatac aaatactgaa ttctccgata tttaatctct
       61 ctgatattgc tataattata ggtatgataa tgatattatt ttcttcttga tgggaggtga
      121 atgtatgtat cctgtaatct tgcgtcttgg aaatttttct ataaggtcct ggggattaat
      181 ggtaatgaca ggaatcatcc tggctgtctt atttgctatg agagaggcaa gtaagagggg
      241 tgttgatccg gaaaatatcc tggacctgtc aatcatcctg attatttccg gtataatcgg
      301 ggcaagggtt ttatatgtcc tggtaaattt caatagttac agggataatc tgggtcttgc
      361 tttttctata aaaacaggag gattagcctg gcctggtgca ttcctttttg cccttatagc
      421 taccatcttc tttatcagat ggcgtaaact ttccttctgg ttgatagtag atatccttgc
      481 tccctatata gcattgggtt attctgtggg caggatagga tgttttctta atggatgctg
      541 ttttggaaag atctgcgagc ctgcctggtg gagtgtctat atgctgggag cgtatagata
      601 tcccacacag ttagtctctt cattgacgtc atttattatc tttctgttcc ttgtaactag
      661 agactggtat gataaacccg ggaaacgttt cctggtttac attatcctgt actgtgtata
      721 caggtttata atagaattct atagatataa cagcacttat ataggaggtc tgagtataac
      781 ccaatggggg atgctgggga cactgattgt gacctccttg gtattgtatg gaaagagttt
      841 ggagtggaaa agaagagcga cttgatctgg tcgtgaaaga gatgcttaca gatctatcta
      901 gggagaagat aaagggcatt atagaggctg gaaaggtctc ggtggatgga agagttatct
      961 gtaaaccgag ttttaaagta aaagatggtt ctgatattag aattgaagaa ttacctgtgg
     1021 aggacaacac tttgcttccg tatgatcttc ctgttgatat agtatatgaa gatgagagta
     1081 tagctgtggt taataaacct cctggacttg tggttcatcc cgcaccaagt gtaaaaagtc
     1141 ctaccctggt caatgccctt gttggtaaga tagaactggc tcctgtaaat aaggagagac
     1201 caggaatagt acacagattg gacagagata cttctggact tatggtaata gccaagactc
     1261 ttcagagtta taatgccctt atcagagaga tgcaggaaag aaggataaaa aaggagtata
     1321 tagcggtggt ttacggaaag gttaagtccg gtatgataaa tgctcctata gggagatctc
     1381 agctgaacag aactaaaatg gctgtatctc cttcaggaag gatagctatt acacattacg
     1441 agttgctgga aatcattgga gactactctc tgataagggt aagaatagag actggaagaa
     1501 cacatcagat aagagttcac ctgtcttata taggtcatcc tgtggttgga gatagagttt
     1561 atgggaggaa agaggatgag actctgatat ccaggcaggc tcttcactct tcaagattgt
     1621 gtttccttca tcctgtgtca gggaagttac tggattttta ttctccgatc cctgaagata
     1681 tcgagaatct tctaaataga ctgagaggaa tgatttgaat agattacttg ccgtttgtat
     1741 tttgatagga attatacata tgaccgacac actgtcactc tctgtgagat gggttgggtt
     1801 aaaggtgggt agagtggtta ctgcctccag tttatttaac atcctggtta ttgtatccag
     1861 aacggctaat ttattacagg ctcctatggt tggaaagatg gttgatctgg caatcaggaa
     1921 tcatcaatcc ttgcctttat taaatgaatt tagacttata
//
LOCUS       DAOTKU010000328         1956 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_46933_length_1956_cov_2.792215, whole genome shotgun sequence.
ACCESSION   DAOTKU010000328 DAOTKU010000000
VERSION     DAOTKU010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46933_length_1956_cov_2.792215"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1768
                     /locus_tag="P5310_07275"
     CDS             <1..1768
                     /locus_tag="P5310_07275"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRU33195.1"
                     /translation="VFTQDWNEIPSEDMKLEIGRYGGTLRLADPSPESGSAPETWCFC
                     REPLFVTPGIDTMGEMKKGNLRGNVFESYKVSKDFKTYTFYMRKGLKWSDGTPVTTED
                     VRFRFEDVYLNEKLTPIFPAWLKVNYNPNEGPGKLKVIDKYTFSISFPKSYPLFIAYL
                     ALRWNSWDTLLIPSHYMKQFHAKYTPLEKLEPLIQKESFGKGEWWRLFWSRNSYSGQH
                     GGPASGFPTLDPWVYKEQPATGIFVFERNPYYFKVDAAGNQLPYIDRVRVQWVTDLKM
                     QTMKIIAGEVDFAVNRTNLTDFPLFKENESAGHYKVALLKQHVTPYDIFLNLTYKDPV
                     WRTVVRNVKFRQALNMAINRNRIIEVVQLGKGSLPTKAKVPFEYDPEKANKILDSIGL
                     NKRDADGWRLGPDGKRFIIPFEVPIWTVGTDKVTELVAEDWRKVGIYTTVKVIDMSLA
                     STRREANENKAMCFWLDWAVYWPLNPMAWACSIDILFDDWGPLWRDWYYSKGKTGEEP
                     PPDIKRFISALERVVATGDDKVREGSMKQVYKMLYDNVYFFPIAESYYPMIYNENFGN
                     IPVNTEYGISAAYSAEQFFFRK"
     gene            1831..>1956
                     /locus_tag="P5310_07280"
     CDS             1831..>1956
                     /locus_tag="P5310_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004082548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRU33196.1"
                     /translation="MLEFIIRRLLLFIPLLFIVSIVSFIIIQLPPGDYLTSYIMQL"
BASE COUNT          622 a          300 c          438 g          596 t
ORIGIN      
        1 agtgtttact caggattgga atgagattcc atcagaagat atgaaacttg agataggtcg
       61 atatggtggg acattaagac tggcagatcc atccccagaa agtggatctg ctccagagac
      121 gtggtgtttt tgtagggaac cactatttgt tacccccggt atagatacta tgggagagat
      181 gaaaaagggc aacctaagag gtaatgtttt tgagagttat aaggtatcaa aggactttaa
      241 aacttataca ttctatatga gaaagggttt gaagtggtca gatgggacac ctgtgactac
      301 tgaagatgta cggttcaggt ttgaagatgt ataccttaat gagaaactta ctcctatttt
      361 tccggcatgg ttaaaagtta attacaatcc taatgaaggt ccagggaagc taaaagtcat
      421 cgataagtat acttttagta tcagtttccc gaagtcctat cctttattta tagcttattt
      481 ggcattaaga tggaattcct gggatacttt actaataccg tctcattata tgaaacaatt
      541 tcatgcaaag tatactcctc tagaaaagtt agagccatta attcaaaagg agagttttgg
      601 taaaggagag tggtggagac tattctggag tagaaacagt tactcaggac aacatggtgg
      661 tccagcatcg ggttttccaa ctcttgaccc atgggtatat aaagaacaac cagcaacagg
      721 tatatttgtt tttgagcgta atccatacta ttttaaggtt gatgcagctg gcaatcaact
      781 tccatacata gatagagtaa gggttcagtg ggttactgat ttaaaaatgc agacaatgaa
      841 gataatagcc ggagaagtag attttgctgt aaacagaaca aatctcacag actttcctct
      901 atttaaggag aatgagtctg caggacatta taaggtggct ttacttaaac aacatgtaac
      961 cccatatgat attttcttaa atctcactta caaagatccg gtatggcgaa ccgtagtgag
     1021 aaatgtgaaa ttcagacagg cattgaatat ggctataaac cgaaacagaa taatagaggt
     1081 tgttcaactg ggtaaaggtt cactgccaac taaagcgaaa gttccatttg aatatgaccc
     1141 agaaaaagcg aataagattt tggattctat aggtctcaat aaacgtgatg cagatggctg
     1201 gcgccttgga ccagatggca agagattcat tattccattt gaagttccga tatggacagt
     1261 gggtacagat aaagttactg agcttgtagc tgaagattgg agaaaggttg gaatttatac
     1321 cacagtaaag gttatagata tgagtcttgc cagtacccgt agagaagcta atgagaataa
     1381 agcgatgtgc ttttggctag actgggcggt atattggcct ttgaatccta tggcttgggc
     1441 ttgtagtata gatatattat ttgatgattg gggaccactt tggagagatt ggtactattc
     1501 taagggaaag actggagaag agcctcctcc agatataaaa agatttatat ctgctctaga
     1561 aagagttgta gctactggag acgataaggt gagagaagga tcaatgaagc aggtatacaa
     1621 gatgctgtat gataatgttt atttcttccc tatagctgag agttattatc caatgatata
     1681 taatgagaat ttcggtaata ttccagtaaa tactgagtat gggatatcag ctgcttatag
     1741 tgcggagcaa tttttcttta gaaagtagtt tccaatctga tagggggcag aaattcttct
     1801 gccccctatt ttcaaaggag ggataacgag atgcttgaat ttattattcg acgtttgtta
     1861 ctatttattc cattattgtt tattgtttct atcgtttctt tcataattat tcagcttcct
     1921 ccaggagatt atttaacctc ttatataatg caattg
//
LOCUS       DAOTKU010000329         1952 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_47056_length_1952_cov_3.663152, whole genome shotgun sequence.
ACCESSION   DAOTKU010000329 DAOTKU010000000
VERSION     DAOTKU010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47056_length_1952_cov_3.663152"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="P5310_07285"
     CDS             complement(<1..588)
                     /locus_tag="P5310_07285"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HRU33197.1"
                     /translation="MKNGLWDSIRGRLIVWFLVISIVPLGVFTYISYSNSKNTIEDVS
                     IEGQKGIAQRLSVRFTDFVYFNSIFVAQSSLAPGIINGIKIRDNNEVMKRLKDYLSLN
                     KYYEAMFWSDMNGKGFTTAGNEVDISDRDYFKAVISTQKSAISEGIISRATGVPVISI
                     AYPVFDETAGDMIGVLGCTVKLDELTEICKSAGLTG"
     gene            complement(600..998)
                     /locus_tag="P5310_07290"
     CDS             complement(600..998)
                     /locus_tag="P5310_07290"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HRU33198.1"
                     /translation="MPIVWNDSLSTGIITIDNQHKELFKRINELLDSSGKTKETINEV
                     ARFLQSYIINHFATEENYMNRTKYPDYPAHKSAHDRYRVEFNQLKEKIEKEGVGLSVS
                     VQMNHLLIDWWLNHINKVDKKMADFIKDKI"
     gene            complement(1218..1553)
                     /locus_tag="P5310_07295"
     CDS             complement(1218..1553)
                     /locus_tag="P5310_07295"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HRU33199.1"
                     /translation="MPSHTPPARIIQLINQIKSHLAMNYGEKIKDIILYGSYARGEAK
                     KDSDIDVLVLVDSSLDPFEVRKSLSDLLFDILLENNELISVVVLHSDFFEEYNYPFIL
                     NVKKDGVKV"
     gene            complement(1566..>1952)
                     /locus_tag="P5310_07300"
     CDS             complement(1566..>1952)
                     /locus_tag="P5310_07300"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HRU33200.1"
                     /translation="LSWNKNLETGINIVDSQHKELVENLNKFFDACMEKRGKEELINM
                     IKFLEKYIVFHFKTEEDIMQRHNFPGYSFHRSKHEEFIKSFTEIKRRFETEGATLEIT
                     TNTTRFLSDWLINHISKIDKQLSSIK"
BASE COUNT          547 a          432 c          288 g          685 t
ORIGIN      
        1 ccctgttaat cctgctgatt tacatatctc agtgagctca tcgagtttta ccgtgcatcc
       61 cagtacgcct atcatatctc ctgcagtctc atcaaataca ggataggcta tgctaattac
      121 cggaacgccg gtagcccttg atatgatacc ctctgatatg gctgattttt gagttgaaat
      181 cacagccttg aaataatccc tgtcagagat atccacctcg ttacctgcag tggtaaagcc
      241 cttcccattc atatccgacc agaacatagc ttcatagtat ttatttagag atagatagtc
      301 cttaagcctc ttcataacct cgttattatc ccgaatcttt atgccattta ttatccctgg
      361 tgctaaagat gattgagcta caaatataga gttgaaatag acgaaatcgg taaatctgac
      421 ggagagcctc tgagcaatac ccttctgccc ttcgatagag acatcctcta tggtattttt
      481 tgagttggag taacttatat atgtgaatac acccagtgga acaatagaga tgacaagaaa
      541 ccatacaata agcctacctc tgattgaatc ccacagacca tttttcattc tatatcctcc
      601 taaattttgt cttttataaa atcagccatc ttcttatcca ctttatttat atggttcaac
      661 caccagtcga taagaaggtg gttcatctgt accgagacac ttaatcctac cccctctttc
      721 tctatcttct cttttaactg attaaactcc actctgtatc tatcatgggc actcttatgg
      781 gcagggtagt caggatattt tgtcctgttc atgtagtttt cttcagtagc aaaatgattt
      841 attatatagc tctgcaggaa ccttgcaact tcatttatag tttcttttgt ctttcccgaa
      901 gaatcaagga gctcattaat tcttttaaat aattccttat gctgattatc gatagtaatg
      961 attcctgttg aaagggaatc gttccagact attggcataa tacttatcac ctcctttatt
     1021 gtactttttc aagataactc tttaaattat gaacaaactt ccatgctatt tttaataaat
     1081 cccttgcttc ctcttcagtt acaataaagc ctatcccata atctccaacc aatctcttgt
     1141 cataggcatc attaagtgac ttccctacct ccctgtcaga aatttctcta tcttctctat
     1201 aaaatccctt atttccttca tacctttacc ccgtctttct tcacattcag tataaatgga
     1261 tagttatatt cttcaaagaa atcagaatgt aaaactacaa ctgagataag ttcattattc
     1321 tcaagcagaa tatcaaacaa aagatcgctc aagctctttc taacttcaaa agggtcaagg
     1381 gaactatcca caagaacaag gacatctatg tcggaatctt ttttagcttc acctcttgca
     1441 tatgagccat acagaataat atccttgatt ttttcaccat agttcattgc cagatggctt
     1501 tttatttgat ttataagttg gattattctg gctggaggcg tatgagacgg cataatattc
     1561 acaccctact ttatactgct gagctgttta tcgatcttac ttatatggtt gatcagccag
     1621 tcggataaga accttgtggt atttgttgtt atctccagtg ttgctccttc cgtctcaaat
     1681 cgcctcttta tctcagtgaa actttttata aattcttcat gtttggacct gtgaaatgag
     1741 tatcccggaa agttatgcct ttgcataata tcctcttcgg ttttaaaatg gaatactata
     1801 tatttctcaa gaaactttat catatttata agttcctctt tgcctctctt ctccatacag
     1861 gcatcgaaaa acttattaag gttttcaact agctctttat gctgactatc cacaatgttt
     1921 atccctgttt ccaaattttt attccaggat ag
//
LOCUS       DAOTKU010000330         1949 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_47179_length_1949_cov_2.457233, whole genome shotgun sequence.
ACCESSION   DAOTKU010000330 DAOTKU010000000
VERSION     DAOTKU010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47179_length_1949_cov_2.457233"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..932
                     /locus_tag="P5310_07305"
     CDS             <1..932
                     /locus_tag="P5310_07305"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRU33201.1"
                     /translation="VENSKKGDYRVIVWNTGDGSNCQIMFNQNYHEDEDIGELIRNKN
                     FRIALSLAINRDEINQLCYLGLGQPRQATVIPSCPTFEEKFAKAYVEYDPKRANEMLD
                     KIGLKRGKDGFRTLQNGKMVALTIEIVSAFGPYVDAAELIKKYWEAVGVKTAIQVQER
                     SLHYTRIRAGLHQVAIWNMDGAFYPQFLTYPYWFLPYGNDSRIAPLSGVWYQTGGKGG
                     EEPKGDLKRAILLYDEAIKTTNPAKRLTLAKEIIRLHSENLWTIGTVGLAPATMGIGV
                     VKNNFRNVPEKALSDVTLNSPSNTHPEQYFFEK"
     gene            1067..>1949
                     /locus_tag="P5310_07310"
     CDS             1067..>1949
                     /locus_tag="P5310_07310"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HRU33202.1"
                     /translation="MKISCCWLYAISKYGYPPRMENVFKALKEMADMGFEYVELEGVR
                     EENLKEVYSNREKIKDHCENLGVKIINFCPIIPDIVSLDDDKRGKARDLFMLAIDIAK
                     YFDCETIQTDSYTPPLEFIGEAPYKESISYGKQYKVKIDPDFSWERLWDVLVDSFRFA
                     SETAKEAGLKFCLEPRVGEIISNTDAILRLMDHVKSDNFGAVLDTGHQNAQKEILPLS
                     VEKLGNRIFYLHVSDNDGKINEHLPLGKGNIDWEGIFLALKKHNYQGYVAIDIGNVPD
                     IEKAYMDSKRFLEDLGKR"
BASE COUNT          674 a          278 c          456 g          541 t
ORIGIN      
        1 ttgtagagaa ttctaagaag ggggattaca gagtaatagt atggaatact ggggatggct
       61 caaactgtca aattatgttt aaccaaaatt atcatgagga tgaagatatc ggagaactta
      121 taagaaacaa aaatttcagg atagccctgt cactggctat taatagagat gagataaacc
      181 agctgtgtta tcttggtctg gggcagcctc gacaggcaac tgtaatacct tcttgtccta
      241 cctttgaaga aaaattcgct aaagcatatg tagaatacga tccaaagaga gctaatgaga
      301 tgctagataa aattggatta aagagaggta aagatggctt ccgtaccttg caaaatggaa
      361 aaatggtggc gcttacaatt gaaatagttt ctgcttttgg tccttacgtt gatgccgctg
      421 aattaataaa gaaatattgg gaagcagttg gtgttaaaac ggcaatccag gttcaagaaa
      481 gatcattgca ttataccagg ataagagcag gacttcatca ggtagccatt tggaatatgg
      541 atggagcatt ctatcctcaa tttcttacat acccatattg gttcctaccc tatggaaatg
      601 attctagaat agctcctctt tctggagtat ggtatcaaac tggaggaaaa gggggagaag
      661 agcctaaagg agatttaaag agggcgatat tactctatga tgaggctata aaaactacta
      721 atccagctaa aaggttgact cttgctaaag aaataattcg tctgcacagt gagaatttat
      781 ggactattgg tacagtagga ttagctcccg caactatggg gataggagta gtaaaaaata
      841 actttagaaa cgtaccagaa aaggctctta gtgatgttac tctcaactct cctagtaata
      901 cacatccgga acaatatttc tttgaaaagt agtttttgtt gatctactca gaaaagagga
      961 ggggcttacc ctcctctttt ctgttacttt tttataatta agttttataa tatttataat
     1021 aagagataat tataaatagg agaattacaa gctaggaggt attattatga agatcagttg
     1081 ctgctggttg tatgcaataa gcaagtatgg gtatccgcca cgtatggaga atgtctttaa
     1141 ggcattaaaa gagatggcag atatggggtt tgaatatgta gaactggaag gggtaaggga
     1201 agagaatctg aaagaggtat acagtaatag agaaaagata aaggaccact gtgaaaatct
     1261 gggggtaaag ataataaact tttgtcctat cataccagat attgtaagcc tggatgatga
     1321 taaaagggga aaagccagag atctatttat gctggcgata gatatagcta aatattttga
     1381 ctgtgagact attcagactg acagttatac cccacctttg gagtttatag gagaggctcc
     1441 atataaggag agtataagtt atggaaaaca gtataaggta aagattgatc cagattttag
     1501 ctgggaaaga ctctgggatg tactggtcga tagcttcagg tttgccagtg aaactgctaa
     1561 agaagcagga ttaaaattct gcctggagcc cagagtaggt gagataataa gcaatactga
     1621 tgccattctc aggcttatgg accatgtaaa aagtgataac tttggagcag ttttagatac
     1681 aggtcatcaa aatgcacaaa aagagattct gccattgtct gtagaaaaat tagggaacag
     1741 gatattctac ctccatgtat cagacaatga tgggaaaatc aatgagcatc tccctttagg
     1801 gaaaggtaat atagactggg aggggatatt cctggcatta aagaaacata actatcaggg
     1861 gtatgtagct atagatattg gtaatgttcc agatatagag aaggcgtata tggattcaaa
     1921 gaggttctta gaagatttag ggaaaagga
//
LOCUS       DAOTKU010000331         1949 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_47180_length_1949_cov_2.414467, whole genome shotgun sequence.
ACCESSION   DAOTKU010000331 DAOTKU010000000
VERSION     DAOTKU010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47180_length_1949_cov_2.414467"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="P5310_07315"
     CDS             complement(<1..414)
                     /locus_tag="P5310_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU33203.1"
                     /translation="MESILYNVGMANRISKIFARDGKAMVLAIDHRQRGIQEGIDNFN
                     NLISLIEGLAPYIDAIMTTKEPLGQIILNGNLRDKGLVLSLNRTGLAGTNFEMDDRSV
                     CTVETALRWGLDGVKLLIKFNRDNLSTNEQLSLLSS"
     gene            complement(427..1449)
                     /locus_tag="P5310_07320"
     CDS             complement(427..1449)
                     /locus_tag="P5310_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRU33204.1"
                     /translation="MDKVRFAIVGCGVIGPTHAEAIKNIDMAELKAVCDIIPERAKAM
                     GEKYGAEWYTDYKELLARKDIDVVNICVPSGLHADIGIEAAKAGKHVLVEKPIDITLE
                     KADALIEACKKAGVKLTVISQHRFDPGIKELKNAIDTGKIGKLVMADAYVKWWRTQEY
                     YESGDWRGTWALDGGGALMNQSVHFVDQLLYLAGPVKEITAYCGTLAHNIEVEDVAIA
                     IIKYKNGALGVIQGSTACYPGFKERLEFHGTNGSVIVEGPLVKDWKIRGEENKEITAA
                     SFSGASDPRAIGIMGHIAQIRDMAEAVLNDRAPSITGEDGRRAVQFILAVYESSRTGK
                     AVAINE"
     gene            complement(1454..>1949)
                     /locus_tag="P5310_07325"
     CDS             complement(1454..>1949)
                     /locus_tag="P5310_07325"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HRU33205.1"
                     /translation="EGMGIEELRKISLERLGELVKIAEKEGAILLHENEKRIYGDIPE
                     RCLDILKSINSPNLKAVFDPSNFIQCNVKPFREAYPLLEEYIEYIHIKDANFSDGVEV
                     PAGEGDGEIKEILKAIKGKGREFFLSLEPHLAHSGQFQGFSGPELFKKASQALKKILS
                     EIER"
BASE COUNT          465 a          513 c          314 g          657 t
ORIGIN      
        1 actagatagc agtgatagct gttcgtttgt agacaggtta tctctgttaa actttatgag
       61 taacttaact ccatccagcc cccatctcag ggcagtttct actgtgcata ctgacctatc
      121 gtccatctcg aagtttgttc ccgccaaccc tgttctgttg agacttaaaa cgagtccttt
      181 gtctcttaag ttaccattca atattatctg accgagaggc tcttttgtgg tcataatggc
      241 atctatatag ggtgctaatc cctctatgag agagattaaa ttgttaaagt tatctatacc
      301 ttcctgtatc cctctctgtc tgtggtctat agccagtacc atagcctttc catctctggc
      361 aaagatcttt gatatcctgt tagccattcc tacattgtaa agaattgatt ccattaagcc
      421 cctcctttat tcatttattg ccaccgcttt tccggtcctg ctggattcgt aaactgcaag
      481 tatgaactgt acagcccttc tcccatcctc gcctgtaatg ctcggtgctc tatcatttaa
      541 gactgcttca gccatatccc ttatctgagc aatatgtccc attattccga tagctcttgg
      601 atcacttgcg ccggagaaac tggcagcagt tatctcctta ttctcttctc cccttatctt
      661 ccagtctttt acaagcggtc cttccactat tacgcttcca tttgttccat ggaattccaa
      721 tctctcttta aagccaggat agcaggcggt tgacccctgt atcacaccaa gtgcaccatt
      781 tttatacttt attatagcta tagccacgtc ttctacctct atgttgtggg caagtgttcc
      841 acagtaggcg gttatctctt taaccggtcc agccagatag aggagctggt ctacaaagtg
      901 gacagattga ttcatcagag ctcctccacc atccagagcc catgtaccac gccaatctcc
      961 actttcataa tattcctggg ttctccacca cttgacatat gcatctgcca taacgagttt
     1021 cccaatttta cctgtatcta tagcattctt taactctttg ataccagggt caaacctgtg
     1081 ctgggatatt acagtcagtt tgacaccagc ctttttgcag gcttctataa gggcgtcagc
     1141 cttttctaaa gtgatatcta taggtttttc gaccagcaca tgtttccctg cttttgctgc
     1201 ttctatacca atatcagcat gtaatccact ggggacacat atatttacca catctatatc
     1261 ttttctggct aaaagctctt tataatcggt ataccattcc gccccgtatt tctctcccat
     1321 cgcttttgcc cgttcgggta taatatcaca taccgctttc aattctgcca tatctatgtt
     1381 ttttatagcc tctgcatggg ttggacctat cactccacat ccaactatgg caaatcttac
     1441 cttatccata ctatcacctc tcaatttcgc tgagaatctt ttttaatgcc tgagacgctt
     1501 ttttgaagag ttctggtcca gaaaagccct gaaactgtcc actatgtgcc agatgtggct
     1561 caagactcag gaaaaactct cttccttttc cttttatagc cttcagaatc tctttgattt
     1621 caccatcacc ctcaccagcc gggacctcca ctccatcaga gaaattagca tcctttatat
     1681 ggatatattc tatatattcc tctaatagag gatatgcttc tctgaatggt ttaacattgc
     1741 actgtataaa attagaaggg tcaaataccg ccttaagatt tggagaattg atacttttca
     1801 gtatatcaag acatctctcc ggtatgtcac catatatcct cttctcattc tcatggagga
     1861 gaatcgcccc ttccttttca gcaatcttta ccagctctcc aagtctttcc agtgagatct
     1921 ttctcagctc ttctatgccc ataccttct
//
LOCUS       DAOTKU010000332         1944 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_47336_length_1944_cov_2.488089, whole genome shotgun sequence.
ACCESSION   DAOTKU010000332 DAOTKU010000000
VERSION     DAOTKU010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47336_length_1944_cov_2.488089"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="P5310_07330"
     CDS             complement(<1..489)
                     /locus_tag="P5310_07330"
                     /inference="COORDINATES: protein motif:HMM:NF017071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HRU33206.1"
                     /translation="MNKGRLRIEWAERQMPVLMSLREDFEREKLLVGIRIGMCLHVTT
                     ETAVLARTLRAGGAEIALCGSNPLSTQDDVVETLSEEHTADVYARRGISEEEYYKNIE
                     QVLSIKPHIIMDDGGDLTTVLHKRWEEFGGYVIGGTEETTTGVNRLLSMDKTKQLRIP
                     VIA"
     gene            complement(482..1537)
                     /locus_tag="P5310_07335"
     CDS             complement(482..1537)
                     /locus_tag="P5310_07335"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRU33207.1"
                     /translation="MKSWQRDILWLLLCISGIILSVWVFYKIRSIIFLFIIVGILAYT
                     VNPSITRLASKLKLKKGLAVGIFYIVIIVPVILLLVLVLPVLFNQISSLVEELPKYYE
                     LISEMVGQIQDKIQTNPKLQDAVNSFLSSIQPQIEKFISSIGLRVLNLIITITSSILT
                     LILAIILNFYFLIDIENIRRWFVNILPADIKDKTLSALDQINISFRSFLKAQMILCLF
                     VGLADGLGVWILGVDYALTLGIIAGFTEIIPYLGPYIGAIPAIILALIISPWKALEIA
                     IWYIAIQQIESYFIVPKVMSKAMGLHPLTVIFSLLVFGKLFGFWGVLFAVPIAAIIKI
                     ILRVYFTDIWKKGGLDE"
     gene            complement(1534..>1944)
                     /locus_tag="P5310_07340"
     CDS             complement(1534..>1944)
                     /locus_tag="P5310_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HRU33208.1"
                     /translation="VCRMERANSTEFDPNTPYPVIDILPEQKNIKAMGGTMRLGGYKC
                     ELKEGTLASKLYNRPYTIERHRHRYEVNNRYRDILESSGLVVSGVYREQDLIEIIELK
                     DHPFFIATQFHPEFKSRPNRPHPLFTGFIKAALL"
BASE COUNT          571 a          402 c          323 g          648 t
ORIGIN      
        1 cgctatcact ggtatcctta actgtttggt cttatccata ctcaacagtc tgtttactcc
       61 agtagttgtt tcttctgtac caccgatgac atatcctccg aattcttccc atcttttatg
      121 gagtactgtg gttaaatctc ccccatcatc cataataata tgtggcttta tcgataagac
      181 ctgttctata ttcttataat attcttcttc acttattccc cttcttgcat atacgtctgc
      241 agtatgttct tctgagagtg tttccacaac atcatcctgg gtgctcagtg gatttgaacc
      301 gcacagtgca atttctgccc ctccagctct aagtgtcctg gctaagacag cagtttctgt
      361 ggttacatgg agacacatcc cgattcttat tccaaccagt agtttttctc tttcaaagtc
      421 ttctcttaag gacattaaaa cgggcatctg cctttcagcc cactctattc ttaatcttcc
      481 tttattcatc taaacctcct tttttccata tatctgtaaa atataccctt aaaatgatct
      541 ttatgatggc agcgattgga actgcaaata aaacacccca aaatccaaaa agttttccaa
      601 aaaccaggag agaaaatatt actgtaagag gatgaagtcc catagcctta ctcataacct
      661 taggaactat aaagtaagat tctatctgct gaattgctat ataccatatg gcgatttcca
      721 gagccttcca tggggaaata attagtgcta gtattatcgc tggtatagca cctatatatg
      781 gtcctaaata gggtataatt tcagtaaacc ctgctataat tccgagggtg agggcataat
      841 ctacccccag tatccagacc cctaacccat cagccagtcc tacgaataaa cacagaatca
      901 tttgagcttt aagaaaagat ctgaaactta tatttatctg atctaaagca ctgagagtct
      961 tatcttttat atctgctggt aagatgttta caaaccatct ccttatgttt tctatatcga
     1021 taagaaaata aaaatttaag atgatagcca gtatcaacgt taggattgaa ctggtgatgg
     1081 ttataattaa attcagtact ctgagtccaa tagaagatat aaacttttcg atttggggtt
     1141 ggatggaaga taaaaaagag tttaccgcat cctgtaactt tggattagtc tggattttat
     1201 cctgtatttg ccctaccatc tctgagatta actcataata ctttggtagt tcctccacta
     1261 aagaagagat ctgattgaat aataccggta atacaagaac gaggaggaga attaccggaa
     1321 ctataataac tatatagaag atacctaccg ctagtccctt ctttaatttt aacttggatg
     1381 ctaacctggt aattgatgga tttactgtat atgctaatat acctacaata ataaatagaa
     1441 atattatact cctgatttta tagaagaccc agactgaaag gattatcccg ctgatacaga
     1501 gaagaagcca gagtatatcc cgttgccagc tcttcatagt aatgctgctt taataaaacc
     1561 ggtaaataga ggatgtggtc tgttgggtct ggatttaaat tctggatgaa actgagtggc
     1621 tataaagaat ggatgatctt ttaattctat tatttctatc aggtcctgtt ctctgtatac
     1681 accacttact acaagtccac ttgactcaag aatatctcta tacctgttat tcacctcata
     1741 tctatgacga tgtctttcta tagtatatgg tctgttataa agtttagatg ccagtgttcc
     1801 ttctttgagt tcacacttgt atcctccaag cctcatcgtt ccacccatcg cttttatatt
     1861 tttctgttct ggcagtatat ctattactgg ataaggggtg ttggggtcaa actcagtact
     1921 gttagctctt tccatacgac aaac
//
LOCUS       DAOTKU010000333         1937 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_47561_length_1937_cov_3.484591, whole genome shotgun sequence.
ACCESSION   DAOTKU010000333 DAOTKU010000000
VERSION     DAOTKU010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47561_length_1937_cov_3.484591"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..925
                     /locus_tag="P5310_07345"
     CDS             <1..925
                     /locus_tag="P5310_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU33209.1"
                     /translation="KTDLAFQVKDYRSIDYMLPKYWIPLPLGFIAIGQDYLGFNSYGV
                     SFIYDAINNVPVFSSYYSMRLKYLTLNLSIDYDGYNDMETLYVYFPLKTSLFSLEDLY
                     VGFSRIGGTNTHNAVFGQWVYSDVDGNDNFITERNASLYTELSLDLNTSAVATIGTWE
                     RRTGKPGEFQPYLGSKFAVGGSNIPDLFSIGGDTGDFILHGYNTGIEEGSFAFIGNIW
                     LDKTILKPYRGLHLGEVFLKEIGARIYLEAGLAGNDIYSSVVKMSVGGELHLSASLGY
                     GILPLEIGIGVAQPLDTTYPTRIYISLEGGF"
     gene            928..>1937
                     /gene="xylB"
                     /locus_tag="P5310_07350"
     CDS             928..>1937
                     /gene="xylB"
                     /locus_tag="P5310_07350"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016483400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HRU33210.1"
                     /translation="MYFMGIDLGTSGVKVLLIDERGDVITKGEGEYTLYTPNPGWTEQ
                     DPLEWWSTTRDVIKKVVAQVNSDEIKGIGLTGQMHGSVFLDEAGNVIRPCILWNDQRT
                     YREVESIMEMFGDKVLEWISNPVLTGFTLPKIIWLKNNEPENFKRLKKVLLPKDFIRF
                     KLTGEYATEVSDASGTALFDVKNRAWSIDMIKAMELLPDLFPKCYESPEITGYVNKKA
                     AEETGLKEGTPVVGGGGDQAAQAVGMGIVHPYDTSVTLGTSGVVFSAIDKVFVDPKLR
                     THTFCHAVPGMWHIMGVMLSAGGSLRWYRDTFGEMEISISRLLGEDPYDILCQEAENI
                     KPG"
BASE COUNT          571 a          317 c          490 g          559 t
ORIGIN      
        1 aaagacagat ttagcctttc aggttaaaga ctatagatct atagattata tgcttcccaa
       61 atactggata ccgttgccgt taggttttat tgccattggt caggattatc ttggatttaa
      121 cagttatggt gtcagtttta tttatgatgc gattaataat gttcctgttt tttcctcata
      181 ctattcaatg agactcaaat atttaacatt gaatctcagc atagattatg atggttataa
      241 tgatatggag actctgtatg tgtacttccc gctgaagact tccctttttt ctctggagga
      301 tttgtatgta ggattttcca gaatcggagg aactaatacc cacaatgctg tctttggaca
      361 gtgggtatac agcgatgtag atgggaatga taattttata actgagagaa atgcctcgct
      421 ctatacagag ctcagtcttg acctaaatac cagtgcagtg gctaccatag gaacgtggga
      481 gagaagaaca ggtaaacctg gagaattcca gccttatctg ggttctaagt ttgcagtagg
      541 aggaagtaat atccctgacc ttttcagtat aggaggagat acaggagatt tcatcctgca
      601 cggttataat actgggatag aggaagggtc tttcgccttt atcgggaata tctggctgga
      661 taaaaccatc ctgaagccat acagggggtt acatttaggt gaagtcttcc ttaaggagat
      721 aggtgccagg atatatctgg aagcaggtct tgctggaaat gacatctata gctctgttgt
      781 aaaaatgagc gttggaggag agcttcatct ctcagcttca ttaggatatg ggatactacc
      841 tttagagata gggataggtg tggctcaacc gttagatact acctatccaa caaggatata
      901 tattagtctg gaaggagggt tttaagtatg tattttatgg ggattgatct tggaaccagc
      961 ggagtaaaag tccttttaat agatgaaaga ggagatgtga taaccaaggg ggagggagaa
     1021 tacaccctgt atacccccaa tcctggatgg acagagcagg acccgctgga gtggtggtct
     1081 acaaccagag atgtgataaa aaaggttgtc gctcaggtaa attcggatga gataaaaggg
     1141 ataggactta caggacagat gcatggttct gtatttttag atgaagcggg caatgttatt
     1201 agaccctgta tactgtggaa tgatcaacgg acatacaggg aagttgaatc tattatggag
     1261 atgtttggag ataaagtact ggagtggatt tccaatccag tactgacagg atttacactt
     1321 ccgaagatta tctggttaaa gaataatgaa ccggaaaatt ttaagaggtt gaagaaggtt
     1381 ttattgccca aagattttat tagatttaaa ttaacaggtg aatatgcaac agaggtttct
     1441 gacgcctctg gaactgcgct ctttgatgta aaaaatagag cctggtcaat cgatatgata
     1501 aaggcgatgg agcttttacc agatttattc ccgaaatgtt atgaaagtcc agagataacc
     1561 ggatatgtaa ataaaaaagc tgccgaagag acaggtctta aagagggaac ccctgtagtt
     1621 ggtggaggag gagatcaggc tgcacaggca gttggtatgg ggatcgtcca tccctatgac
     1681 acatcggtaa ctcttggtac atccggggta gtattctctg ctattgataa ggtttttgtg
     1741 gaccctaagt taagaaccca tacgttctgt catgctgttc ctggtatgtg gcacataatg
     1801 ggtgtaatgc tctcagctgg tggttctctg aggtggtaca gggatacgtt cggtgaaatg
     1861 gagatcagta tctccagatt actgggggaa gacccttacg atatcctctg ccaggaggca
     1921 gaaaatataa agcctgg
//
LOCUS       DAOTKU010000334         1933 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_47706_length_1933_cov_3.674121, whole genome shotgun sequence.
ACCESSION   DAOTKU010000334 DAOTKU010000000
VERSION     DAOTKU010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47706_length_1933_cov_3.674121"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..794
                     /locus_tag="P5310_07355"
     CDS             <1..794
                     /locus_tag="P5310_07355"
                     /inference="COORDINATES: protein motif:HMM:NF013705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HRU33211.1"
                     /translation="KILHIKGQIFPVTLEDVLTGIEREIEIDRKEICDICNGNGLEPG
                     TQPEKCPYCNGTGEIRSVRNTIFGQMVNIAACPSCRGTGKIITHPCHQCGGRGFVSRR
                     RKVLVKVPAGVEDGVRLRLRGEGDTGINGGPAGDLFVFVKVEPHPLFKRLGADLEYQA
                     AIDVFDAILGTEIEVPTLEKSREKMNIPSGTQFNSTFRLRGRGLPYLNNGGRGDLIVR
                     VKIDVPQKLSERERELLEEIASIRGKKINKDGKSFFDKVKNVFGG"
     gene            795..1673
                     /locus_tag="P5310_07360"
     CDS             795..1673
                     /locus_tag="P5310_07360"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HRU33212.1"
                     /translation="MSRFWVEVSITYNKKAFPNLDLEDIISSKLWDINSSGVAIDSSS
                     NLVRITGYFPVDALLYKRLEELELSLGEYILSGLKFNFSMSEESEWQKFGNFEPFRIG
                     KFYVIPEVNDNCTIPSDLIPIYIKPGMAFGTGLHPTTRMCVNLLEDLSLEDKVGIDVG
                     TGSGILAICMLKLGCSYVIGIDNDLIAISDARENATRSGVLDRLDLRLGDLLNDYNES
                     KVDVITANLFPQLITPIFDKVSSVLNSGGHIIVSGFSNEESGEIIDLARSYSLILKEK
                     IVEEDWSALLFIYDTF"
     gene            1660..>1933
                     /locus_tag="P5310_07365"
     CDS             1660..>1933
                     /locus_tag="P5310_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU33213.1"
                     /translation="MTLFRIFLEKVSEIIHLSDREILNHISARRLSIDDEVIVCDREG
                     HSYRYRVREKGYNSVVLEMIEPVEFLPPVRKVVYAQSLLKPRKLEPI"
BASE COUNT          608 a          280 c          471 g          574 t
ORIGIN      
        1 ccaagatact tcacattaaa ggccaaattt ttcctgttac tctggaggat gtccttaccg
       61 ggatagaaag agagatagaa atagacagaa aagagatttg cgatatatgt aatggaaatg
      121 gtttagaacc aggaacccag cctgagaagt gtccttactg taacgggaca ggtgaaatta
      181 gatctgtccg taatacaatc tttggacaaa tggttaatat tgctgcctgt ccatcctgca
      241 gaggaacagg caagattatt acacatccct gtcatcagtg tggtggcaga ggttttgtct
      301 ccagaagaag aaaggttctg gtaaaggttc cagctggagt agaggatgga gtaagactca
      361 gattgagagg tgagggagat actggaatta atggtggacc agctggggac ctctttgtat
      421 tcgtaaaagt agaaccacac cctcttttta agagactggg agcagatctg gaatatcagg
      481 ctgctataga tgtgtttgat gctattttgg gaacagaaat tgaagttcct accctcgaaa
      541 agagtaggga aaagatgaat attccatctg gaactcagtt taattctact tttagattaa
      601 gaggaagggg gctcccatat ttaaacaatg gtggaagggg tgaccttata gtcagggtaa
      661 agatagatgt cccccagaag ctcagtgaaa gggagagaga gttgttagaa gagatagcca
      721 gtatacgtgg taagaagatt aataaagatg gaaagtcttt ttttgataag gtaaagaatg
      781 tctttggagg ttaattgtcc aggttttggg tagaggtaag tataacttat aataagaagg
      841 cttttccaaa tttagatctg gaagatataa tctccagtaa actctgggat attaattctt
      901 ctggagtagc tatagattct tcaagtaatc ttgtaaggat aactggatac tttcctgtgg
      961 atgctctact gtataagaga ttggaagagc tggaactctc tctgggagag tatatactat
     1021 ctggtctgaa attcaatttc agtatgtctg aagagagtga atggcagaag ttcggtaatt
     1081 ttgagccgtt tcggattggg aaattctacg ttatacctga agttaatgat aactgtacga
     1141 ttccttcaga cttaataccc atatatataa agcctggcat ggcttttgga acgggactgc
     1201 atcccactac aagaatgtgt gtaaacttat tggaagatct ttctttagaa gataaagttg
     1261 ggatagatgt gggaacagga agtggaatac tggctatatg tatgttaaaa cttggttgtt
     1321 cttatgtgat agggattgat aatgatctta tagctatatc agacgccaga gaaaatgcca
     1381 caagaagcgg agttctggat agattagacc tcaggttggg agacctgttg aatgattata
     1441 atgagagtaa agtggatgtt attacggcta acttatttcc tcaactcata actcctattt
     1501 ttgataaggt ctcttctgtt ttaaattcag gagggcatat tatagtcagt ggtttctcca
     1561 atgaagagag tggagaaatt atagatttag cccgttctta tagccttatt ctgaaagaga
     1621 aaatagtgga agaagactgg tcagctctat tatttatcta tgacactttt taggatcttc
     1681 ttagagaaag tatcagaaat catccatctc tctgatagag aaattttgaa tcatatatct
     1741 gccaggagac tctctatcga tgatgaggtt atagtctgtg atagagaggg tcatagctat
     1801 aggtatagag taagagagaa gggatataat agtgtcgtcc tggagatgat tgagcctgta
     1861 gagtttttac caccggtgcg aaaagtggta tatgcacaat ctcttttgaa acctaggaag
     1921 ttagaaccaa taa
//
LOCUS       DAOTKU010000335         1932 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_47756_length_1932_cov_2.384656, whole genome shotgun sequence.
ACCESSION   DAOTKU010000335 DAOTKU010000000
VERSION     DAOTKU010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47756_length_1932_cov_2.384656"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="P5310_07370"
     CDS             complement(<1..214)
                     /locus_tag="P5310_07370"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRU33214.1"
                     /translation="MLKGIIPAIITPFKGEDIDFLAFEKLLNFHINSGVGGFFVAGTN
                     GEGPLLSKEEKSLLFKKAVEIVNKKVP"
     gene            complement(249..1055)
                     /locus_tag="P5310_07375"
     CDS             complement(249..1055)
                     /locus_tag="P5310_07375"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HRU33215.1"
                     /translation="MMKLSFSTLGCPNWDIKRIVESAKRYGYNGVELRGAGRQHISFE
                     ISPLERKEIKTIFEENGLEICCISAYTRFSTPVREERESNIDTLVRYCELARELNSPL
                     VRSFIGDVPEDIKETDWLEYIPEALHSAGERIKGSGVDVVIETHDYFSTGKLIAQLIE
                     RAKSENIKALWDVWHPYRSGEPPEETISYLKNYLRHMHIKDARIIGEDWQLVLPGDGD
                     IPLKKITGLLKDTGYNGYLSFEWEKMWHPEIPEPEIAFPRYVEYMNSIIV"
     gene            complement(1052..>1932)
                     /locus_tag="P5310_07380"
     CDS             complement(1052..>1932)
                     /locus_tag="P5310_07380"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HRU33216.1"
                     /translation="KDLIMLLSRYTSPGKKLNWLSSQIKMRELENFRCLREEDTREME
                     IPKETSIHFSGIDWVITRRSWEDRLGPVLAVKAGNNGEPHNQIDVGHFIFYVYGQQFL
                     HDLGSGIYTKDYFREGRYKSPFCNAEGHSLIFIDGESEGIGEEFKGKIVSFESGTDED
                     RIVIDLTKAYFSENVRNITRTLHFLKDRIDGQLILEDLVSLNRDSRIESRIQINGEIE
                     RPGEKSFIVKGKYGNLYVDIKEPLDCKVELGELSNLEFHDGTVRSFKFLDIVTYGKDA
                     RFRIIFTPFRDRGELE"
BASE COUNT          528 a          479 c          281 g          644 t
ORIGIN      
        1 caggaacctt tttatttact atctcaactg cctttttaaa gagtaaactc ttctcctctt
       61 ttgaaaggag gggaccctcg ccatttgtgc cagccacaaa gaacccacca accccactat
      121 taatatgaaa attcaacaac ttctcaaatg ccagaaaatc tatatcttca cctttaaacg
      181 gggtaataat agcaggtatt atccctttaa gcatacggtt cctccttaat cagaaattta
      241 agttattatt atacaataat cgaattcata tattccacat acctgggaaa tgctatctct
      301 ggttctggta tctcaggatg ccacatcttt tcccattcaa aagagagata tccattatag
      361 cctgtatctt taagcagacc ggtgatcttc ttcaacggta tgtcaccatc ccctggcaag
      421 accagttgcc agtcctcacc aattatcctg gcatccttta tatgcatatg tctcaggtaa
      481 tttttaagat aacttatggt ctcctcagga ggttccccac tcctgtatgg atgccataca
      541 tcccagaggg cttttatatt ctcactctta gccctttcta ttaattgggc tataagttta
      601 ccggtggaga aataatcgtg agtctcaata acaacgtcta ccccagaccc tttaattctt
      661 tctcctgcag aatgaagcgc ttctggaata tattcaagcc aatctgtttc tttaatatct
      721 tcaggtacat ctcctataaa gctccttaca agaggagaat ttaactctct ggcaagttca
      781 cagtatctaa ctaaagtgtc tatattactt tccctctctt ctctgacggg ggtggaaaat
      841 ctggtatagg cacttataca gcatatctcc aatccattct cttcaaaaat tgtctttatc
      901 tcttttctct ccagaggaga gatctcgaaa gatatgtgct gtctaccagc tcccctcaat
      961 tctaccccat tgtaaccata cctcttagca gactcaacaa tcctttttat atcccagtta
     1021 gggcacccta aagtactgaa agagagtttc atcattctaa ctcaccccta tctctgaatg
     1081 gagtaaatat aatacggaac cttgcatctt taccataggt aactatatcc aaaaacttaa
     1141 aacttctcac agttccatcg tggaattcca gattactcaa ttctccaagt tccaccttac
     1201 aatccagtgg ctcttttata tctacataaa gattaccata tttcccctta actataaagc
     1261 ttttctctcc aggtctttct atctccccat ttatctgtat cctggattct attctgctat
     1321 ctctatttaa ggagactagg tcttcaagta taagctgtcc atctattcta tccttcagga
     1381 aatgaagggt tctggtaata tttctgacat tctcagagaa ataggctttt gtcaggtcta
     1441 taacgattct atcttcatcc gtacctgatt caaaagatac tatcttccct ttaaactcct
     1501 ctcctattcc ttcactctct ccatctataa atatcaggct gtgaccttct gcgttgcaga
     1561 acgggctctt atatcttccc tctctgaaat aatcctttgt ataaattcct gaacccagat
     1621 catgaaggaa ttgttgccca tatacataga atataaaatg tccaacatct atctgattat
     1681 gcggttctcc attatttccg gcttttaccg ccagaacagg tccaagcctg tcttcccagc
     1741 ttctcctggt tataacccag tctataccgc taaaatgtat ggatgtctct ttggggatct
     1801 ccatttccct ggtatcttcc tccctgagac atctgaagtt ctccagctct ctcatcttta
     1861 tttgactaga taaccagttt aattttttgc cagggctagt atacctggaa aggagcatta
     1921 taaggtcttt at
//
LOCUS       DAOTKU010000336         1928 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_47869_length_1928_cov_2.786439, whole genome shotgun sequence.
ACCESSION   DAOTKU010000336 DAOTKU010000000
VERSION     DAOTKU010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47869_length_1928_cov_2.786439"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..807
                     /locus_tag="P5310_07385"
     CDS             1..807
                     /locus_tag="P5310_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRU33217.1"
                     /translation="MSGEIIFYRDGTSVELHKLDPKGPEYRSIRGKEIAMIFQEPMTS
                     LNPVYSVGEQIMEAIILHQNVDEKEARKRAIEMLDKVGIPSPEKRIDEYPHQLSGGMR
                     QRIMIAMALSCNPSLLIADEPTTALDVTIQAQILELMKALQKDFNTSIMIITHNLGVV
                     AELANEVMVMYLGEVVEHGNVKDIFKKRLHPYTNGLFKSTPHLGKNAKQPLTPIQGSV
                     PEPINLPKSCRFLPRCNFRFERCDEDPPLFILENGHRVKCWLYEDSGVKI"
     gene            804..1829
                     /locus_tag="P5310_07390"
     CDS             804..1829
                     /locus_tag="P5310_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRU33218.1"
                     /translation="MKPVLLDVKGLKKYFPVKRGFLRKTVGYIKAVDGIDLYIREGET
                     LGLVGESGCGKTTAGRAILRLIEPTDGKIYFRSSIRAGNYDGMVDITGLTQEELKPLR
                     QEMAIIFQDPISSLNPRMTVKDIIREPLEIHNKGTAKEQEDRVVELLESVGLTAEHMS
                     RYPHEFSGGQRQRIGIARALALNPRLIICDEPVSALDVSIQGQVLNLLVELQRNLGLT
                     YLFISHDLAVVQYISDRVAIMYLGQVVELGPTDLLYSNPLHPYTEALLSAIPIPDPEY
                     QRERIILQGGVPSPLNPPSGCYFHPRCIYAQDICSQEHPEWRELRPEHFVACHLAEKL
                     NLKGIGS"
BASE COUNT          643 a          320 c          435 g          530 t
ORIGIN      
        1 atttctggag agattatatt ctaccgtgat ggtacctctg tagaactcca caaacttgat
       61 ccgaaaggtc ctgaatatcg ttctataaga ggaaaagaga tagctatgat atttcaggag
      121 ccgatgacat ctttaaatcc agtatattca gtaggtgaac agataatgga agctattata
      181 ttacatcaga atgtagatga gaaagaagca cggaaaagag ctatagagat gctggataaa
      241 gttggaatac ccagcccaga aaagcgcata gatgaatatc ctcatcagtt aagcggtggt
      301 atgagacagc gtataatgat agcaatggct ctatcatgta acccaagtct tttaattgct
      361 gatgaaccca ccaccgcctt agatgttact attcaggcgc aaatactgga attaatgaag
      421 gctctgcaga aagatttcaa tacatctatt atgattatca ctcataatct aggtgtagtg
      481 gctgaattgg caaatgaagt gatggttatg tatctgggag aggttgtgga acacggaaac
      541 gtaaaagata tattcaaaaa gagactgcat ccctatacca atgggctttt caaatctacc
      601 ccacatttag gaaagaatgc gaaacagcca ctgacaccga ttcagggttc tgttccggaa
      661 ccgataaatt taccaaagag ttgcagattt ctaccaagat gtaattttag atttgagaga
      721 tgcgatgagg atccaccatt attcatcctc gaaaacggac atagggtaaa atgctggtta
      781 tatgaagata gtggggtaaa gatatgaaac ctgtactcct ggatgtaaaa ggattaaaga
      841 aatactttcc tgtaaagaga ggttttttaa ggaaaacagt aggatatata aaagcggtag
      901 atgggataga cctctatata agagagggag agacactggg attggttggt gaaagtggtt
      961 gtggcaagac taccgctgga agagctattc tgagactgat agaacctact gatggaaaga
     1021 tttactttag aagctctata agggctggta attatgacgg tatggtagat attacaggtc
     1081 ttactcagga agaattaaaa cccttgagac aggagatggc gattatattt caagacccta
     1141 tatcctcact taatccaagg atgacagtaa aagatataat cagagaacca ctggagatac
     1201 ataataaagg aactgcaaaa gagcaagagg atagagttgt agaactttta gaatcagtgg
     1261 gtcttaccgc agagcatatg tccaggtatc ctcatgaatt ttctggagga cagcgtcagc
     1321 gtataggtat agcaagggca ttggctttaa atcccaggtt gataatatgt gatgaacctg
     1381 tctctgctct tgatgtttcc attcaaggac aggttttaaa tttgttagta gagttacaga
     1441 gaaacttggg attaacatat ctctttatct ctcatgatct tgcagttgtt cagtatatct
     1501 cagatagggt agcaattatg tatcttggtc aggtagttga attaggacct actgatcttc
     1561 tatattccaa tcctctacat ccatacacag aggctttact ctcagctata ccgataccag
     1621 atcctgaata tcagagggaa agaataatac ttcaaggcgg agttccttct ccacttaatc
     1681 ctccaagtgg ttgttatttc catccgagat gtatatatgc ccaggatata tgcagtcagg
     1741 aacatcctga atggagagaa cttcgtccag aacattttgt tgcctgtcat ttagctgaga
     1801 aactcaatct taaagggatt ggaagttaat tatgatggga ttagaaagaa aaacagttac
     1861 agaagaagag ggccgatctg ttattataaa ggctgaggag ttacgaaaaa ctaaacgata
     1921 ccaggagg
//
LOCUS       DAOTKU010000337         1925 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_47969_length_1925_cov_4.192513, whole genome shotgun sequence.
ACCESSION   DAOTKU010000337 DAOTKU010000000
VERSION     DAOTKU010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47969_length_1925_cov_4.192513"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="P5310_07395"
     CDS             <1..462
                     /locus_tag="P5310_07395"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HRU33219.1"
                     /translation="REGDEIVYIDKVESKHTVRMYSRIGARAPLYCTALGKCLLAFSS
                     EDSIKRWTSKESFPQKTENTIKTGDELTREIERVKSQGYALDREEFEQGVKCIGVPIL
                     NRTGKAIAAISISIPTFRITEEKIEEFIPLLLKTSSEISEKLRYLQEVKGP"
     gene            459..1604
                     /locus_tag="P5310_07400"
     CDS             459..1604
                     /locus_tag="P5310_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRU33220.1"
                     /translation="MISLDVNYAGIRLKNPLIVSSAGIVGYAHQIKKAEDYGAGAVVM
                     KSFFEEEICREAPTPRFKILKRGYNGNSSSTLYSYEQASELGPEEYAEELSKAKSLVK
                     IPIFASLNCVTPDMWNRMSHLVEEAGADGIELNVSCPHGVHIMEGRALKDEIKKALEA
                     TQEVRIPRIVKLSPQTSDPMIEANVVEENNGSGIVMFNRFTGLDIDLEKEAPILHGGY
                     AGHGGAWAIYYVLRWISTVYPHLHIPIAASGGVTDGKDAIKCILAGATTVQICTVVIL
                     KGYEVIQKINKEIFEFLDEKGYQSLDEIRGKVTGKILSYKEVDRRRLYMAYIDQEKCN
                     QCNICYKACYSGAIEKRDGRIYVNSNCVGCGLCAELCPRRAIELVGL"
     gene            complement(1599..>1925)
                     /locus_tag="P5310_07405"
     CDS             complement(1599..>1925)
                     /locus_tag="P5310_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019721618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase PcrA"
                     /protein_id="HRU33221.1"
                     /translation="ERRLSYVGMTRAKRYLYLTAAEIRTLYGHTNSNEISRFIKEIPE
                     EYIEPVTKGEIAFERGERVWHSRWGEGIIQEVRPLEDGETELIVAFNDIGKKHLLARY
                     AGLEKL"
BASE COUNT          587 a          333 c          465 g          540 t
ORIGIN      
        1 agagaggggg acgagattgt ttacattgat aaggtggaga gtaaacatac agtcaggatg
       61 tactccagaa taggtgccag ggctcctcta tactgtaccg ctctgggaaa atgtcttctg
      121 gcgtttagtt ctgaggatag tattaaaaga tggacatcta aagaatcatt tcctcagaag
      181 acagagaaca ccataaaaac aggagatgag cttaccagag aaattgaaag ggttaagtct
      241 caaggatatg ccctggatag agaggaattt gaacaggggg tgaagtgtat aggtgttcct
      301 atccttaaca ggacgggtaa agctatagct gctataagta tatcgatacc gacttttagg
      361 ataacagagg agaagataga ggaatttatt cctctccttc tcaagaccag ctctgaaatc
      421 tcagaaaaat taagatatct acaggaggta aaaggacctt gatcagtctg gatgtaaatt
      481 atgcaggaat aagattaaaa aatccattaa tcgtttcttc tgcaggtata gttggatatg
      541 cacatcagat taagaaggca gaggattatg gtgccggggc ggtagttatg aaatctttct
      601 ttgaagagga aatctgtaga gaggcaccaa cccccaggtt taagatattg aagagaggat
      661 ataacggtaa ttcttcatct actctttatt cgtatgaaca ggccagtgag cttggtcctg
      721 aagagtatgc cgaagagtta tcaaaagcta aaagtcttgt aaagattcca atttttgcca
      781 gtttgaactg tgtaacaccc gatatgtgga acagaatgtc ccatctggta gaagaagctg
      841 gggcagatgg gatagagtta aatgtcagtt gtccccatgg tgtccatata atggaaggaa
      901 gggcattaaa agacgagata aagaaggcac tggaggcaac ccaagaggtc aggatcccga
      961 ggattgtcaa gctaagtcct cagacgagtg atccgatgat agaagcaaat gtggttgaag
     1021 aaaataatgg tagtgggatt gtaatgttta atagattcac cggattagac atagatctgg
     1081 agaaagaagc cccaattctt catggtggtt atgcgggaca tggtggagcc tgggctatct
     1141 attatgtatt gagatggata tctaccgttt atccccattt acatatccct atagctgcca
     1201 gtggtggagt aactgacggg aaagatgcca ttaaatgtat actggcaggg gcgactactg
     1261 ttcagatatg tacagtggtt atattgaagg gttatgaagt aattcaaaag ataaataaag
     1321 agatatttga atttcttgac gaaaagggat atcagtcact ggatgaaatt cgtggcaaag
     1381 taacaggtaa gattctctcc tataaggaag tggatagaag aagactttat atggcttaca
     1441 tagaccagga gaagtgtaac cagtgtaata tctgttataa agcctgttac tctggcgcta
     1501 ttgaaaagag ggatggaagg atttatgtaa actcaaactg tgtgggttgt ggactctgtg
     1561 cagaactttg ccccaggaga gctatagagc tggttggatt ataatttttc tagccctgca
     1621 tatctggcta agagatgctt cttcccgata tcgttaaagg ctactattaa ctctgtttcg
     1681 ccatcctcta acggacggac ttcctgaatt atcccttccc cccatcttga gtgccacacc
     1741 ctttctcctc tttcaaatgc gatttctccc ttggtaaccg gttctatata ttcttccggt
     1801 atctctttta tgaatctcga tatctcgtta ctatttgtat gcccgtagag cgttcgtatc
     1861 tcagccgcag taagatagag gtatctttta gctctggtca tacccacata ggagagtctt
     1921 ctttc
//
LOCUS       DAOTKU010000338         1912 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_48450_length_1912_cov_2.564890, whole genome shotgun sequence.
ACCESSION   DAOTKU010000338 DAOTKU010000000
VERSION     DAOTKU010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48450_length_1912_cov_2.564890"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /gene="recA"
                     /locus_tag="P5310_07410"
     CDS             <1..394
                     /gene="recA"
                     /locus_tag="P5310_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014905788.1"
                     /note="catalyzes the hydrolysis of ATP in the presence of
                     single-stranded DNA, the ATP-dependent uptake of
                     single-stranded DNA by duplex DNA, and the ATP-dependent
                     hybridization of homologous single-stranded DNAs; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HRU33222.1"
                     /translation="YGGNPEVTPGGRALKFYASIRLDIRKIDNVKEGDEALGNRVRVK
                     VVKNKLAPPFRQAEFDIIYGRGISRLGEIVDLGVEANVVTKAGSWYSYNSERLGQGKQ
                     KAVEYLSQHLDMANEIERQILEKSGVLG"
     gene            413..>1912
                     /gene="rny"
                     /locus_tag="P5310_07415"
     CDS             413..>1912
                     /gene="rny"
                     /locus_tag="P5310_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005550497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HRU33223.1"
                     /translation="MIWDILIGLIAFLIGGSLGYIYRKNKAEGILKNAERVADEIIEK
                     AKAEGEKKKLEIIVSAKDEAEKIKNETDRQIRQKRSELQKQERRLLQRELSIEKRQEG
                     IEKKEKALQAREEMIAETQKEIELLKEQELRDLERIASMTMEEAKNMLISNIEAEAKR
                     EATARLYEIEMELKEETDRKAREIVSLAIQRCASDEVASSTVTVVNLPSEEMKGRIIG
                     REGRNIRTIEMLTGVDLIIDDTPEVVVLSSYDPIRREIARLSLEKLIQDGRIHPARIE
                     EMVEKAQQEVESRIKEEGEKAIFEAKITRVHPEIVRALGRLIFRTSYGQNVLQHSREV
                     SRLASMMASELGIDPTLAKRAGLLHDIGKALDYDAEGPHALLGAEFARKYNENETVVN
                     AIASHHGEETPNSIEAVLVQAADAISAARPGARKETLEAYIKRIKRLEELAVSFEGVE
                     KAYALQAGREIRVIVQPEKLDDLSSFELARELAKKIEDDLEYPGQIRVIV"
BASE COUNT          679 a          261 c          559 g          413 t
ORIGIN      
        1 atatggcgga aacccagagg tgacaccggg gggaagggct cttaaatttt atgcttcgat
       61 aaggcttgat ataagaaaga tagataatgt aaaagaaggc gatgaggctc ttggtaacag
      121 ggtaagggtt aaggtggtta agaataaact tgccccacca tttagacagg cagagtttga
      181 tataatttac ggaagaggaa tctctagatt aggagagata gtagatctgg gggtagaagc
      241 taacgtggta accaaagcag gttcctggta ttcttacaat agtgaaagat tggggcaggg
      301 aaagcagaaa gcggtagagt atctatccca gcatttagat atggctaatg aaatagagcg
      361 ccagatactt gaaaagtcgg gggtcctggg atgaatagtg aggtgattta ctataatttg
      421 ggacatatta ataggattaa tagctttcct gattggggga agtcttggtt atatctatcg
      481 gaagaacaag gcagaaggta tattaaagaa tgcagagagg gtagcagatg agataataga
      541 aaaggctaag gctgagggag agaagaagaa gctagagatt atagtctcag caaaagatga
      601 ggcagagaag ataaagaatg agacagatag acagatcaga cagaagagaa gtgagttaca
      661 gaaacaggag aggagattac tacagcgaga gctttctata gagaagagac aggaaggtat
      721 agaaaagaag gagaaagcct tgcaggcgag agaagagatg atagcggaga ctcagaaaga
      781 gatagagctt ctgaaggagc aagaattgag agatctggag agaatagcca gtatgactat
      841 ggaagaggct aagaatatgc tgatatccaa tatagaggct gaggcaaaga gagaagctac
      901 agctagactt tacgagatag agatggaatt aaaggaagaa acggatagaa aagcaaggga
      961 gatagtctct ctggcgatac agcgttgtgc ttctgacgag gtggcatcat caactgtaac
     1021 tgtagtaaat ctccccagtg aggagatgaa ggggaggata ataggtagag aaggcaggaa
     1081 tataaggact atagagatgt taactggagt ggacctaata atagatgata cgccagaagt
     1141 ggttgtttta tccagttatg atcctataag aagggagata gcgaggttat cccttgaaaa
     1201 acttatacag gacggtagaa tccatcctgc tcgtatagaa gagatggtgg aaaaggctca
     1261 gcaggaagtt gagtccagga taaaggaaga aggagaaaag gctatttttg aggcaaaaat
     1321 aaccagggta caccccgaga tagtaagggc gctcggaaga cttatattta gaaccagcta
     1381 tggacagaat gttttacagc attccagaga agtatccagg ttagcaagta tgatggcatc
     1441 agaattaggg atagatccta ctcttgcaaa aagggcaggg ttacttcacg atataggaaa
     1501 ggctttagat tacgatgcag aaggaccaca tgcattgctt ggggcagaat ttgcacggaa
     1561 gtataacgaa aatgagactg tggtaaatgc catagcaagt catcatggag aggagacacc
     1621 taacagcatt gaggctgttt tagttcaggc tgccgatgcc atatctgcag caagacccgg
     1681 ggcaaggaag gaaacactgg aggcttatat aaagcgtata aagagattgg aagaactggc
     1741 ggtatcgttt gaaggggtag agaaggctta tgcactgcag gctggaagag agataagggt
     1801 tatagtacag ccagaaaagc tcgatgacct atcgtcattt gaattggcaa gagaactggc
     1861 taagaagata gaggatgatc ttgaatatcc ggggcagata agagtgatag tt
//
LOCUS       DAOTKU010000339         1909 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_48544_length_1909_cov_3.324703, whole genome shotgun sequence.
ACCESSION   DAOTKU010000339 DAOTKU010000000
VERSION     DAOTKU010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48544_length_1909_cov_3.324703"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..865)
                     /locus_tag="P5310_07420"
     CDS             complement(<1..865)
                     /locus_tag="P5310_07420"
                     /inference="COORDINATES: protein motif:HMM:NF040068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HRU33224.1"
                     /translation="MNYPFDLLYRIRKPASYIGNEYNIIRKDFKDKIKIALAFPDTYE
                     VGMSNLGFSIIYSIINSREDSLAERIFAPWVDMEEALRKRRIPLLTLESGKTISDFDI
                     LAFSIPYELNYSNILTILDLCGLPLLSSEREGLPLVIAGGTCTSHPSPIIPFIDCFIV
                     GDGEEVINGIIDAFKEWKNNSEQKGELLESLSGIEGIYVPGISRYVKRRIVEDINTVQ
                     YPTSPLVPNTEIVHDRAVVEIMRGCTRGCRFCEAGFLYRPVRERSIENIKRLSEEILR
                     STGYGEIGFLSL"
     gene            complement(862..>1909)
                     /gene="rodA"
                     /locus_tag="P5310_07425"
     CDS             complement(862..>1909)
                     /gene="rodA"
                     /locus_tag="P5310_07425"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HRU33225.1"
                     /translation="LILLAFILSGIGIIAIYSATYWKGPDLWDKQFISVLLTIPVVII
                     FRYIDYKILVRFAPYIYLVSLLFLGATLVLASPVKGARRWLYIGGFNFQPSELIKLAL
                     ILVLADLIEAKKGEWSELFLFITCLGLTLIPFLITAMQPDLGTAVILMVIFMVTIFVS
                     QIRIRYIIFLIGLGITSIPLAWKYLLMDYQKERILTFLDPNRDPLGAGYHIIQSKLAI
                     GSGGLLGYGFLNGPQNRLKFIPEQHTDFIFSLIGEEFGFIGSLLVIILFLFLFTVGLY
                     ISSRSDTKAGLLMGVGIISMLCVHVFINIGMTIGLLPVTGIPLPFISYGRSGLMVNMM
                     GIGILSSISKRRVR"
BASE COUNT          624 a          423 c          316 g          546 t
ORIGIN      
        1 tcagagataa aaaaccaatc tccccatatc cggtagatct gagaatctct tccgacagtc
       61 tctttatatt ctctatactt ctctccctta ctggacgata gaggaaccca gcctcacaaa
      121 atctgcatcc cctagtacac ccacgcatta tctctaccac agctctatca tgcactatct
      181 ccgtgtttgg tacaagcgga gaagtggggt attgaactgt gttaatatct tctactatcc
      241 ttcgtttgac atatctactg atgccaggaa catagatgcc ttctataccg cttaaagact
      301 ctaaaagctc tcctttttgt tcgctattat tcttccattc tttaaatgca tctattattc
      361 cgttaataac ctcttcccca tcaccgacta taaagcaatc tataaaggga atgatgggag
      421 agggatgtga ggtacaggta ccaccagcta ttacaagtgg cagtccctcc ctctcagaag
      481 ataacaaagg aagtccacag aggtctaaaa tggtaagtat attggaatag tttaattcat
      541 aaggtataga aaaagcgagg atatcaaaat cactgatagt cttaccactc tccagagtca
      601 gaagtggaat cctccgtttc ctcaaagcct cttccatatc tacccatggg gcaaatatcc
      661 tttctgccaa agaatcttcc ctgctattta taatactgta aatgatagaa aaacccaagt
      721 ttgacatccc cacttcatag gtatcgggga aggcaagcgc tattttaatc ttatctttaa
      781 aatcttttct gattatattg tattcattcc cgatataact tgccggtttc cttattctat
      841 aaagtagatc aaatggatag ttcatctcac tctcctttta cttatggaag aaaggatccc
      901 aattcccatc atattgacca tcaatccact ccttccataa cttatgaacg gcagtggtat
      961 ccctgtaact ggtaataaac ctatggtcat acctatattt ataaatacat ggacacagag
     1021 catagaaata attcccacac ccatcagtag acctgcttta gtatcactcc tggatgatat
     1081 atataacccc actgtaaaca ggaaaagaaa aaggataata accagtaaag atccgataaa
     1141 accgaattct tcccctatta aagagaatat aaaatcggta tgctgttccg gaatgaattt
     1201 taatctattc tgtggtccgt taagaaatcc gtatcctagg agtcctccag agcctatagc
     1261 taacttggac tgtataatat gataaccggc accaagaggg tctctattcg gatctaaaaa
     1321 ggtgagtatc ctctcctttt gataatccat cagaaggtat ttccaggcta atgggatact
     1381 tgtaatcccc aatcctatca gaaaaatgat atatcttatt cgaatctgag aaacaaagat
     1441 agtcaccata aatataacca tcaagataac agcagtccca aggtctggct gcatagcagt
     1501 aatcaggaac ggtataagtg ttaatcctaa acaggttata aacaagaaga gttcactcca
     1561 ttcacccttt ttggcttcta taagatcagc taaaactaat ataagagcca attttattaa
     1621 ttctgacggc tgaaagttaa atcctcctat atataaccat cttctggcac cctttacagg
     1681 tgaagccaga accagggtag ccccgagaaa cagtagagaa accagataga tatagggggc
     1741 aaaccttact agtatcttat aatctatata tctgaagatt ataactacag gaatagtcag
     1801 caggactgaa ataaactgct tatcccagag atcaggaccc ttccagtagg tagcactata
     1861 tattgctatg atacctatac ctgacaagat aaaggctaag agtatcaga
//
LOCUS       DAOTKU010000340         1903 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_48739_length_1903_cov_3.112554, whole genome shotgun sequence.
ACCESSION   DAOTKU010000340 DAOTKU010000000
VERSION     DAOTKU010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48739_length_1903_cov_3.112554"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="P5310_07430"
     CDS             complement(<1..220)
                     /locus_tag="P5310_07430"
                     /inference="COORDINATES: protein motif:HMM:NF013112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HRU33226.1"
                     /translation="MNIYIHTLGCFKNEVDSSGISTVLKRAGFNMTSLDEADMVILNT
                     CGFIRPAKEEAINSILRFIDYKKSRGVKL"
     gene            complement(217..969)
                     /locus_tag="P5310_07435"
     CDS             complement(217..969)
                     /locus_tag="P5310_07435"
                     /inference="COORDINATES: protein motif:HMM:NF024855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HRU33227.1"
                     /translation="MNEKKLGDILREKRESLNLTVEELAGITKLKKSVILALENGDYG
                     ELQESVYIRGFFKIYASAIGLDYKELYPKLEKELRAIGKIPEEEEKPGRKSVIFIVII
                     AIIIAVPVLYYIISSSSPGVKEPESNVEITQPVTEPVAQPPVKEPEPAAKEPEPNIEE
                     TTTEEFVTPVKKEVAVEIKGLGYSWVRVIVDDEKVFEGFINSGDLYSWKGEKNIVIRA
                     GNAGAVNVVLNGKDIGVLGKSGEVVEREFIPE"
     gene            complement(966..1856)
                     /locus_tag="P5310_07440"
     CDS             complement(966..1856)
                     /locus_tag="P5310_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRU33228.1"
                     /translation="MIEGIFSLELLSSALRMSVPLVFASLGGVYSERGGVVNIALEGI
                     MLMGAFGAVVGTYFTNNPWIGLLVAMLFGLIIALIHAVVSITFRINQIISGTALNMMA
                     TGLTTFLSRLIFQAAGSSPHIEGMIQWQFPGTSFKISPLIVIMILAVILSQIIIFKTR
                     YGLRLRACGENPYAADSLGIDVIKIRYSGVIMSGILGGLGGAYLSLEHARVFVEGMTT
                     GRGFIALAGMIFGRWTPVGSFSACFLFGLAEALRMRLEGFGIPTQFIQMIPYLVTMGV
                     LIGVVGRAVAPAYDGIPFEK"
BASE COUNT          562 a          472 c          281 g          588 t
ORIGIN      
        1 taagctttac acctctagat tttttataat ctataaatct cagaatagag ttgatagctt
       61 cttcttttgc aggtcttatg aatccacagg tgttaaggat aaccatatct gcctcatcca
      121 gactggtcat attaaaccct gctctcttca ataccgtaga aattcccgat gaatccacct
      181 cgtttttaaa acatcccagg gtatgtatgt agatattcat tctggaataa attctctttc
      241 tacgacctct ccacttttac ctaaaacccc aatatcctta ccattcaata cgacatttac
      301 agctcccgca ttaccagccc tgataactat attcttctct cctttccagg agtataaatc
      361 gccactattt ataaatcctt caaatacctt ttcgtcatcc acaataaccc tgacccagga
      421 atatccaaga ccttttatct ccacagcgac ctcttttttt actggagtta caaactcttc
      481 ggtagtagtt tcttctatat taggttcagg ttctttggct gctggctcag gctctttgac
      541 aggtggctga gctacaggtt cagttacagg ctgagttatt tccacattac tctcaggttc
      601 ttttacccca ggagatgaag aagatatgat atagtacagt actggaactg ctataataat
      661 agcgatgatt actataaaaa tgacggattt tcttcctggt ttttcctcct cttcagggat
      721 cttgccaatc gcccttaact ctttttcaag ctttggatac agctctttat aatctaaacc
      781 aatggctgaa gcatatattt tgaagaatcc acgtatatat acagattcct ggagttctcc
      841 gtagtcacca ttttccaatg ccagaatcac actcttcttc aatttagtta ttccagctaa
      901 ttcttcaacg gtaagattaa ggctctctct tttttccctc agtatatctc ctaacttctt
      961 ttcattcact tctcaaaggg tatcccgtca taagctggcg ctactgccct cccaactact
     1021 cctattaata cacccatagt aactaaatat ggtatcatct gtataaactg ggtagggata
     1081 ccaaatccct ccaatctcat tcttaaagcc tctgccagtc caaataaaaa gcaggcagaa
     1141 aaagaaccga caggcgtcca tcttccaaat atcatacctg ccagggcaat aaatcctcta
     1201 cctgtagtca taccctctac aaataccctg gcatgttcta aacttaaata tgcccccccc
     1261 agcccaccaa ggattccact cataataaca ccgctgtatc tgatctttat aacatctatc
     1321 cccagactat ctgcagcgta tggattttct ccacaggctc taagtcttag accgtatctg
     1381 gtcttaaaaa taattatctg gcttaatatc actgctaaaa tcattataac tataagtgga
     1441 gagattttaa agctggttcc aggaaactgc cactgaatca taccttcaat atggggtgat
     1501 gaaccagctg cctgaaagat taatctggag agaaatgttg tgagaccggt agccatcata
     1561 ttcagtgcag ttccacttat tatctgattt atcctgaagg ttatggagac aaccgcatgt
     1621 ataagggcaa taataagacc aaacaacatg gctactaaca atcctatcca ggggttattg
     1681 gtaaaataag tacccaccac tgctccgaaa gctcccataa gcattatccc ttctaaagct
     1741 atatttacaa cccctccacg ttcactgtaa actccaccaa gagatgcaaa aactagagga
     1801 acagacatcc ttaatgctga ggacaaaagt tctaatgaaa aaatcccctc tatcattctt
     1861 cttccctttc cagtctcatt aataatctga agataatctc att
//
LOCUS       DAOTKU010000341         1896 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_48985_length_1896_cov_3.174905, whole genome shotgun sequence.
ACCESSION   DAOTKU010000341 DAOTKU010000000
VERSION     DAOTKU010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48985_length_1896_cov_3.174905"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1869)
                     /locus_tag="P5310_07445"
     CDS             complement(<1..1869)
                     /locus_tag="P5310_07445"
                     /inference="COORDINATES: protein motif:HMM:NF025774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRU33229.1"
                     /translation="MEVDNLNLIPGSHIVWIYSGDKGYTSGLVNLIKDGLERNEKILF
                     IGFKDDINRFSEIIKDIEKKDDIYTFTVAERLIFPIKDIRRLLEEKKPYTFRIISVID
                     IFNEPPNAKYITNLSIEIESICKNSHIWICFYDKNRLNTARFLPLILSHQYLIIDNRI
                     FRNYLYLSPEKILEDDIDYMLNRIEKNLIERDNLWMSYKETSDLYKALFENTGTATLV
                     IEDFLITSVNSAFEELTGFKREEVENKKLWTELIPYREDLERMIGYRELRIKDPELAP
                     KVYETRIRDKAGRIKDVILNVDIIPGTKRFVWSLTDITEIKRTGRLLRMISLGNQELI
                     KAEDEKDLKDRILKIVKEYGRYRDVFFDQTPEGSTTLRVITDRELTDSENDILRELSQ
                     DVDYGIRAIRRRKLLRYNEQRYRTIFKNTPVALMEEDLTEAIEYLDSLRKSGIEDIEG
                     YFRDHPEERYRCLDTVKILSINDAALRLYMVKDKKELEDNFLRIIPESEREKSFKEIL
                     ALFEGSRGGEFTTVNYTLYGEEKNILLKWFSLPQLPEDEERRHDLIVALVDITEEKRL
                     SDILRENLDRLRKTFDQTIELISYMGELKDPYTAGHQKNVSELACRIARKMGIAEDI"
BASE COUNT          484 a          435 c          293 g          684 t
ORIGIN      
        1 tatatcttcg gctattccca tcttcctggc gattctgcaa gctaactctg aaacattctt
       61 ctggtgacct gcagtgtacg ggtctttcag ctctcccata tacgatataa gctctatggt
      121 ctggtcaaat gtctttctca gtcgatctag attttccctc agaatatcgg agagtctctt
      181 ctcctctgta atatcaacca gcgctactat taaatcgtgg cgtctttctt catcttcagg
      241 tagttgcggg agagaaaacc attttagcag tatatttttt tcctccccat acagtgtata
      301 gttgacagtt gtgaattctc cccctcttga accttcaaag agagccaata tctctttgaa
      361 agacttttct ctttcagatt ctggtattat cctgaggaag ttatcctcca gttccttttt
      421 atcctttacc atgtataacc tcagtgccgc atcatttata ctgaggattt taaccgtatc
      481 cagacacctg tatctctcct caggatgatc tctgaaatag ccttcaatat cttctatacc
      541 tgattttctt aaactatcaa gatactctat cgcctcagtg agatcttctt ccataagcgc
      601 taccggagta tttttgaata ttgtcctgta tctctgctcg ttgtatctta agagttttct
      661 cctccttata gcccttatcc cgtaatccac atcctgtgat agctctctca gaatatcatt
      721 ctcactatct gtaagttccc tgtctgtaat aaccctgaga gtggtagacc cttcaggtgt
      781 ctgatcaaag aagacatctc tatatctccc gtattctttc acgatcttta agattctatc
      841 cttaagatct ttttcatctt cagcctttat aagctcctga ttccccagag agatcatcct
      901 taaaagcctg cctgttcttt taatctctgt tatatcagtc agggaccaga caaacctttt
      961 tgtccccggt attatatcaa cattaagtat cacatctttt atccttcctg ccttatccct
     1021 tatcctggtc tcataaacct ttggggcaag ctcagggtcc ttaatcctga gttctctgta
     1081 gccgatcatc ctttccaggt cttccctgta tgggataagt tctgtccaga gtttcttatt
     1141 ctcaacctct tctcttttaa aacctgtgag ctcttcaaag gcactgttta ccgatgttat
     1201 aagaaaatcc tctataacaa gtgtcgccgt cccggtattc tcaaatagag ctttatacag
     1261 gtctgaagtc tctttataag acatccagag gttatctctt tcgatgagat tcttttctat
     1321 cctgtttaac atataatcga tatcatcttc tagtatcttt tctggagata gatacagata
     1381 attcctgaat atcctgttat ctataatgag atactggtgg gatagaataa gagggaggaa
     1441 tcttgcggta ttaagcctgt ttttatcata gaaacatatc cagatgtggc tatttttaca
     1501 gatagattct atctcgatag aaagatttgt aatatattta gcattcggag gctcattgaa
     1561 gatatctata acacttatta tcctgaaggt ataaggtttt ttctcttcca agagccttct
     1621 tatatctttt atggggaaga tcagtctctc agctactgta aatgtatata tgtcatcttt
     1681 cttttcgata tcttttatta tctcgctgaa tctgtttata tcatctttaa atccaataaa
     1741 aagtatcttc tcatttctct ctagaccatc ctttataaga ttaactagac cggatgtata
     1801 tcccttatca cctgaataga tccagacgat atgactgcca ggtattagat tcagattatc
     1861 cacttccatc aactccagct atataatagc cagcca
//
LOCUS       DAOTKU010000342         1890 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_49205_length_1890_cov_3.132970, whole genome shotgun sequence.
ACCESSION   DAOTKU010000342 DAOTKU010000000
VERSION     DAOTKU010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49205_length_1890_cov_3.132970"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            297..>1890
                     /locus_tag="P5310_07450"
     CDS             297..>1890
                     /locus_tag="P5310_07450"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HRU33230.1"
                     /translation="MVEDKVTKTFKVKGMSCAGCARKIENNLNKIDGVYATVNIATEK
                     AIVTYDPEKAREKELFDLVESLGYKLIDDTEEDSQMDLVKKRFLISVAFTLPGIVIMI
                     IDMLNLLEIPYIDLIQIFISVPVIFWAGFHIIKSSIVSISHRTFGMDVLISLGTLASF
                     MTGVIRLLGFNIANYALVGAMIMTIHLLGRYLETLAKGRATRAISQLLELGVKKANVI
                     RDGEEIEVPVEELKIGDVVIVRPGGKIPSDGVIVEGDTYIDESMITGEPIPVKKTIGD
                     SVIGATINQSGVIKVRITRLGKDTFLSQIIRLVEEAQGSKVPIQELADRITGIFVPVV
                     LVISLGVFLFWLIYPEAGRAILVNFSSIFPWINLENNTISQAISVMVSTLVIACPCAL
                     GLATPTALIVGSGIGAEKGILIRSGKSIEIMKDVNTIVFDKTGTLTKGRPEVTYIWSK
                     LDNEEFLRIIASIENNSEHPLARAIVEYAIKKNIPLVKVESFVSIPGRGIRASLDGKE
                     YLVGSVNFLIENGYDPSIYGERL"
BASE COUNT          594 a          348 c          420 g          528 t
ORIGIN      
        1 tggctatttt atagaataac tcttccagga tatttctcaa tattttttct gaactctcaa
       61 tggtctacga aaatgtcatc taaaatcttc taaatataca agattaattt gtatcccgta
      121 ggggcaattc atgaattgcc cctacaatcc tgctacgcac taccgatcaa gcgtcatggc
      181 ttttatgtgt gacgatatca caaaacgata acattgaact ttactttttc aatagaatat
      241 gttatatttt attaaccccc ccccataggg gggagggtag aaaggagaat gaattaatgg
      301 tagaggataa ggtcacaaaa acctttaaag ttaagggtat gtcctgtgcc ggatgtgcca
      361 ggaagataga gaataatctt aataagatcg atggggttta tgccacagta aatatagcca
      421 ctgaaaaagc catagttaca tatgaccccg aaaaggctag agaaaaggaa ctatttgacc
      481 tggtagaaag tctcgggtat aaactcatag acgatacaga ggaagattcg cagatggatc
      541 tggtaaaaaa gagatttctt atctctgtag cattcacctt gcccggaatt gtaattatga
      601 taatcgatat gctgaatcta ttagagattc catacatcga cctgatccag atttttatat
      661 ccgtacccgt aatcttttgg gcaggatttc atataataaa atcttccata gtctccatct
      721 ctcacaggac attcggtatg gacgtcttaa taagtttggg tacactcgca tcctttatga
      781 caggggtcat aagactacta ggttttaaca tcgcaaacta tgcactcgtt ggggctatga
      841 taatgaccat ccacctgctt ggcagatatc tggagacact ggctaagggt agagccacca
      901 gagccataag tcaactctta gaacttgggg tcaaaaaggc taatgttata cgggatgggg
      961 aggagataga agtccctgtg gaagaactta aaattggaga cgtggtaata gtaagacctg
     1021 gcggtaagat accctctgat ggagttatcg ttgaaggaga tacctatata gatgagtcta
     1081 tgattaccgg agaaccgatt ccagtgaaga aaactattgg agatagtgtt ataggcgcga
     1141 caataaacca atcaggggta ataaaggtaa ggattaccag gctgggcaaa gatacattcc
     1201 tctcccagat aataagactg gttgaggaag ctcaggggag taaagtaccc attcaggaat
     1261 tggctgatag aattaccggt atatttgttc ctgtagttct tgtcatatct ctcggtgtct
     1321 ttctattctg gctgatatat ccagaggcag gcagggctat actggtaaac ttttcttcaa
     1381 tattcccctg gattaatctt gaaaacaaca ctatctctca ggctatatct gttatggtct
     1441 caacccttgt aatagcatgt ccctgcgcct tggggttagc cactccaact gccctcatag
     1501 tgggaagtgg tatcggtgca gagaaaggga tacttatcag gtctggtaag tctatagaga
     1561 taatgaagga tgtgaatacg atagtcttcg ataaaacagg cacattaaca aagggaagac
     1621 cagaggtcac atatatatgg tctaaactgg acaatgaaga gttcctgaga attattgcct
     1681 ctatagaaaa taactctgaa catcccctgg caagagctat agttgagtat gctattaaaa
     1741 agaatatacc actggtaaaa gtggaatctt ttgtttccat accaggtagg ggaataagag
     1801 cctctttaga tggaaaagag tatctggttg ggagcgtaaa cttcctcatt gagaatggct
     1861 atgacccttc catctacgga gagagactcg
//
LOCUS       DAOTKU010000343         1886 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_49368_length_1886_cov_2.480066, whole genome shotgun sequence.
ACCESSION   DAOTKU010000343 DAOTKU010000000
VERSION     DAOTKU010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49368_length_1886_cov_2.480066"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /gene="alr"
                     /locus_tag="P5310_07455"
     CDS             complement(<1..729)
                     /gene="alr"
                     /locus_tag="P5310_07455"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HRU33231.1"
                     /translation="MEANILEIDISNLKSNIEVIKGFIGPYVKFLAVVKADAYGHGIK
                     LVSRYLDNVDRFGVANVEEAIKLRENGISKPILIMGDIFPSDIKDILYYNITPSVSSI
                     EIAKLISKKGLEENRKVPIHIKVDTGMGRFGFLVEELLMSIDKILELPNLELEGIFSH
                     FSAAGVDKNYVDLQFRRFIELISVLERRRIFFSLRHIANSPATLIHPYTTMDMVRIGL
                     AMYGINPTKSQLPVELSPVMSLKSR"
     gene            complement(771..1628)
                     /gene="rsmI"
                     /locus_tag="P5310_07460"
     CDS             complement(771..1628)
                     /gene="rsmI"
                     /locus_tag="P5310_07460"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HRU33232.1"
                     /translation="MGKLYVVATPIGNLKDITCRAIEVLNDCDLIAAEDTRHASILLK
                     HYNIKTRLVSYHKYNEEEISRMLISYMSEKDMDIALISNAGTPCISDPGYRVVSEARR
                     RGLEVIPIPGPSAVMAALSVSGVPINHFLFLGFLPKKESQREQIIRDIKRKQIETVVI
                     YESPKRIISLAELLAREMPSSNVCFFCELTKMFERAFTGRIEDVLERLKNDPKAKLGE
                     YTVIIHYRSEEMVENTEITPEALLIDTMLKEKTTLKEAVEIVSKKFNLPKNYVYKKAL
                     ELKEYISKI"
     gene            complement(1631..>1886)
                     /locus_tag="P5310_07465"
     CDS             complement(1631..>1886)
                     /locus_tag="P5310_07465"
                     /inference="COORDINATES: protein motif:HMM:NF018758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU33233.1"
                     /translation="DVCFIFKFLPTAERVKKGASIELLEKIKSQFIIVSFPLKSIGGK
                     RKGMLENYSKIFEPVFTQRYIIMKRFILEEEVFYILRRGK"
BASE COUNT          502 a          394 c          317 g          673 t
ORIGIN      
        1 cctggatttt aagctcatta ccggtgatag ttcgactgga agctgtgatt ttgttgggtt
       61 tataccgtac attgccagtc caattctgac catatccatt gtggtgtaag ggtgaatcag
      121 ggtagctgga ctgttagcta tatgtcttag agaaaagaaa attcttctcc tctctaaaac
      181 tgaaattaac tctatgaacc gtctaaactg taggtctaca taatttttat ctactcctgc
      241 agcgctgaaa tggctaaata tcccttctaa ctccagattt ggtaactcca gtatcttatc
      301 tatggacatt aagagctcct ctactaagaa gccaaatctc cccatccctg tatctacctt
      361 aatatgaatg gggacctttc tgttttcctc tagacccttc tttgatatga gtttcgctat
      421 ctcgatgctg gagactgaag gggttatatt gtagtagagg atatccttta tatctgaggg
      481 gaagatatct cccataataa gaattggctt tgaaatacca ttttctctta gctttatagc
      541 ctcttctaca ttagctaccc caaatctgtc cacattgtcc agatacctgg ataccagctt
      601 aattccatgt ccataggcgt cagcctttac aacagcaagg aatttaacat agggacctat
      661 aaaccccttt attacctcta tattagattt gagattagag atgtctattt caagtatatt
      721 tgcttccata tagatagtat attatatctt ctatctaaat gtaaatcact ttatatctta
      781 ctgatatatt cttttagctc taaagccttc ttgtatacat agtttttggg aaggttaaac
      841 ttcttggata ctatctctac tgcttccttt agagttgttt tttctttaag catcgtatct
      901 ataagtagtg cctctggggt tatctcagtg ttttccacca tttcctcact tctatagtgg
      961 attattactg tatactctcc caatttagcc tttgggtcat tttttaatct ttccaggaca
     1021 tcttctatcc tccctgtaaa cgctctttca aacattttgg taagttcaca aaagaaacat
     1081 acattagagg acggcatctc tcttgccagt agttctgcca gagatatgat tctttttgga
     1141 gattcgtata tgacgactgt ttctatctgt ttccttttta tgtcccttat aatctgttct
     1201 ctttgagact ctttcttagg aagaaatccc aggaataaga agtgattgat cggaaccccg
     1261 gatacagaga gagcagccat aactgcagat ggaccaggga tgggaataac ctcaagtcct
     1321 cttctccttg cctcactgac aaccctgtac ccaggatctg agatgcaggg ggttccagca
     1381 ttggatataa gggctatatc catatctttc tccgacatat agctgataag catcctagat
     1441 atctcttcct cgttatattt gtgataagag actaatctgg tttttatgtt gtagtgtttc
     1501 agcagaatag atgcgtgtct tgtgtcttca gcagcgatta aatcacaatc attaaggact
     1561 tctatagccc tgcaggttat atcctttagg tttcctatag gtgtcgctac cacgtacaat
     1621 ttccccacta ctatttccct ctccttaaga tatagaagac ctcttcctct aggataaatc
     1681 tcttcatgat aatatatctt tgtgtgaaga ctggttcgaa tattttgcta tagttttcca
     1741 gcatcccttt tcttttaccg ccaatacttt ttaatgggaa tgaaacaatt ataaattgac
     1801 tttttatctt ttctaaaagc tcaatagaag ctcccttttt tactctttct gctgttggga
     1861 gaaatttaaa tataaaacat acatct
//
LOCUS       DAOTKU010000344         1882 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_49517_length_1882_cov_3.755884, whole genome shotgun sequence.
ACCESSION   DAOTKU010000344 DAOTKU010000000
VERSION     DAOTKU010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49517_length_1882_cov_3.755884"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(124..>1882)
                     /locus_tag="P5310_07470"
     CDS             complement(124..>1882)
                     /locus_tag="P5310_07470"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRU33234.1"
                     /translation="EDTKEPFSSNTGGRGWQYGGTWRRAWLGLSDQWGPNKICVEHLL
                     MFDKSGTKVLPNIAESWNVSRDARVYTFKIREGIKWSDGTPLTTDDVMFWYEDVILNK
                     ELTPNVPLWLTSGGKTCEIEKVDTYTFRVKFAQPSPLFLINICKQGGNAFFLPKHYMK
                     QFHPKYTSKEEIEKKAKEAGFQFWYQLFDARGMNSNAWLQNPQLPVLYPWRAASSPTA
                     TTMILERNPYYWKVDPAGNQLPYIDKIAQALLENREMIVMKAISGEIDMQMRHMSLSD
                     YTLLMENKSKGGYRVLQWKQGVGADVMICLNQNVKDPVLRKLFEDRRFRQALSIAINR
                     EEINQLVYLGLGEPRQCSLISGVAFYDPEWEKMWAEYDPKRASEILDKMGLNKKDKEG
                     FRLRPDGKTLTLTITFPSGVFGAWSDVLEMVEKYWKAIGVKVALQPVERSLYVTRCNA
                     GDLEIGVWNFDRNAAVMSDPGRLLGTVTDGPWAPLYGQWYSTGGKGGEEPKGDIKKIY
                     ELWDKVTVTADENQRDRYFKEIINLHKRNIWMIGTVGELPQPVVVKNNFRNVPDGLVW
                     DDTLRAPKNARPEQFFFKQ"
BASE COUNT          480 a          477 c          328 g          597 t
ORIGIN      
        1 taaatgattc cctccttcct gaatctcaac ttttatatta atttatatta agtataacat
       61 agcgcattat ctaaaaagaa aaaggggcaa gtttatagct tgcccccagg attaaatgtc
      121 tctttactgt ttaaagaaga actgctccgg tctggcattc tttggcgctc tgagagtatc
      181 atcccagaca agtccatccg gaacattcct gaagttattc ttcaccacca ccggctgagg
      241 cagttctcct accgttccta tcatccagat gtttctctta tgcagattta taatctcttt
      301 aaagtatcta tctctctgat tttcatctgc tgtaactgta accttatccc agagctcata
      361 aatctttttg atgtctcctt tgggttcctc tccaccctta cctccagtag aataccactg
      421 accatagaga ggtgcccagg gtccatctgt tacagtacct aaaagtcttc ctggatctga
      481 cataacagca gcattcctgt caaaattcca tacaccaatt tcaaggtctc ccgcattgca
      541 tctggtcaca tacagtgaac gttctacagg ctgtaatgcg acctttactc caatagcttt
      601 ccagtatttc tctaccattt caagaacatc tgaccatgca ccaaatacac cgctggggaa
      661 tgttatggtt agagttaatg tcttaccatc tggtctaagt ctgaaacctt ccttgtcttt
      721 cttgttaagt cccatcttat ccagtatttc actagctctc ttggggtcat actctgccca
      781 catcttctcc cactccggat cgtagaatgc tactccactt ataagggagc actgccttgg
      841 ttcacccagc cccaggtata ccagctggtt aatctcctct ctatttatag ctatagacaa
      901 tgcctgtctg aatcttctat cctcaaataa cttgcggagt acagggtcct ttacattctg
      961 attcagacat atcatcacat cggcacctac accctgcttc cactgaagta ccctgtatcc
     1021 tcctttagat ttgttctcca taaggagggt ataatctgaa agactcatat gtctcatctg
     1081 catatctatc tcaccagata ttgccttcat cacaatcatt tctctattct ctaaaagagc
     1141 ctgggctatt ttatcaatat atgggagttg attcccagcc gggtccacct tccagtagta
     1201 tgggtttctc tcaagtatca tggtagtagc agtcggagaa ctggctgctc tccagggata
     1261 aagtacagga agttgagggt tttgtaacca tgcgttggag ttcattcctc ttgcatcaaa
     1321 taactgatac cagaactgga aaccagcctc ttttgccttc ttttcaatct cttctttaga
     1381 tgtatatttt ggatggaact gcttcatata atgcttgggt aagaagaacg cattacctcc
     1441 ctgcttacag atatttataa ggaagagggg acttggctgg gcgaacttca ccctgaacgt
     1501 ataggtatct accttttcta tctcgcaggt tttaccacca gaggtaagcc acaggggaac
     1561 gtttggggta agctctttat ttaaaatgac gtcctcatac cagaacatta catcatcggt
     1621 ggttaacggt gtcccatctg accactttat accttctctg attttaaatg tataaaccct
     1681 ggcatctctg gagacattcc agctctcagc tatattgggt aataccttag ttccactttt
     1741 atcgaacatc aaaagatgct caacacatat cttgtttgga ccccactgat ctgagagtcc
     1801 aagccaggct ctacgccagg ttccaccata ctgccaacct cttccaccgg tgttactact
     1861 aaagggttct ttggtatcct ct
//
LOCUS       DAOTKU010000345         1874 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_49802_length_1874_cov_2.392523, whole genome shotgun sequence.
ACCESSION   DAOTKU010000345 DAOTKU010000000
VERSION     DAOTKU010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49802_length_1874_cov_2.392523"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1061
                     /locus_tag="P5310_07475"
     CDS             <1..1061
                     /locus_tag="P5310_07475"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR00045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HRU33235.1"
                     /translation="RALPDSDITVLPIADGGDGTIEVLVEATSGQFIEKYVTGPLGEK
                     VKASFGILGDGKTAVIEMARSSGLALVPKEKRNPAITTTYGVGELIKEALERGVKRIL
                     VGIGGSATNDGGAGMAQALGAKLLDKEGKELPFGGLALKNLASIDLSGFHPLAKDCEV
                     IVMADVKNPLCGPRGASYVYGPQKGGTEELIEELDKALFNFAQVIRRDLGKDILDLPG
                     SGAAGGLGGGLVAFLDAKLKPGIDVVLETLGIEEKLNGVDLAISGEGKIDGQTIFGKG
                     PAGIAKKVKEKNIPVILFGGSVDDDAEILKEEGLVDAIFSITRGPVSLEESIKHTRGQ
                     LEWISYQIGGLIKALTSK"
     gene            1136..1711
                     /gene="hisB"
                     /locus_tag="P5310_07480"
     CDS             1136..1711
                     /gene="hisB"
                     /locus_tag="P5310_07480"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006567180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HRU33236.1"
                     /translation="MTREASAIRTTKETDVRVRLNIDGSGNTSIDTGIGFFNHLLTSF
                     AFHGRFDLDITARGDLDVDGHHLCEDTGIVLGDVFNKALGDLKIVRFGYSTIPMDDAL
                     IISSVDVSGRPYFETDNLFKDSFIGTFQTEWLIEFLRAFSINSRMTLHVVKLRGDNSH
                     HIAECTMKSIAISLREAVKRTEEVLSTKGVL"
     gene            1714..>1874
                     /locus_tag="P5310_07485"
     CDS             1714..>1874
                     /locus_tag="P5310_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008538431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HRU33237.1"
                     /translation="MITIIDYGAGNLFSVKKALEYFNADVQISDNGKIILKSDGIILP
                     GVGAFGAGI"
BASE COUNT          589 a          304 c          498 g          483 t
ORIGIN      
        1 taagagccct ccctgatagt gatattacgg tccttccaat agcagatggt ggggatggaa
       61 ccatagaggt cctagtagag gcaaccagcg gacagttcat agagaagtat gttaccggac
      121 ctttaggaga aaaggtaaag gcaagctttg gtatccttgg agatggaaaa actgcggtaa
      181 tagagatggc aagatcttca ggcttagcac tggtcccaaa agaaaagaga aatcctgcca
      241 ttaccacaac atatggtgta ggagaactga ttaaagaagc gctggaaaga ggagtaaaaa
      301 gaatactggt tggtataggt ggaagcgcca ccaatgatgg tggggctggg atggctcagg
      361 ctctgggagc taaactgctg gataaagaag gtaaagagtt gccatttggt ggtttagccc
      421 tcaagaacct tgcatccata gatttgagtg ggtttcatcc attagcaaaa gactgtgaag
      481 ttatagttat ggctgatgta aaaaatccgt tatgcggacc tagaggggct tcatatgtat
      541 atggaccaca aaaaggtggg acagaagaac ttatagaaga actggataaa gccctgttta
      601 attttgctca ggtgataagg agagaccttg gaaaagatat actggacctc cctggctctg
      661 gagcggcagg aggactgggg ggcggactgg tggctttctt agatgcaaag ttgaaacccg
      721 gaatagatgt tgtactggaa acgctgggga tagaagagaa attaaatggg gtagacctgg
      781 caataagtgg agagggaaag attgacggac agacgatatt cgggaaaggt ccagctggaa
      841 tagcaaaaaa ggttaaagag aaaaatattc cggtgatact ttttggtggc tccgtcgacg
      901 atgatgcaga gatacttaaa gaagaaggat tggttgatgc tatcttctcc ataaccaggg
      961 gtcctgtaag cctagaagaa tcaataaaac ataccagagg acagttagag tggatttcct
     1021 atcagatagg gggattaata aaggctctta catcaaaata gccaggttgt tttctcatac
     1081 aggtttatag tataatagct taagtgtata tttgaagaag gagatgcagt tgagtttgac
     1141 cagagaggct tcagcgatta gaaccactaa agagacagat gtaagagtaa ggttgaatat
     1201 tgatggaagt ggtaatacca gcattgatac tggtattgga tttttcaatc atctgcttac
     1261 ctcttttgcc tttcacggtc ggtttgacct agatattact gccagaggag acttggatgt
     1321 cgatggtcat catctctgtg aggataccgg gattgtcctg ggtgatgtct tcaataaagc
     1381 cctgggagac ctgaagatag tcagatttgg atattctact attcctatgg atgatgcact
     1441 tatcatctcc agtgttgatg taagcggaag accatacttt gagactgaca atctatttaa
     1501 agatagtttc atagggacat ttcagacaga atggctgata gagttcttaa gggcattcag
     1561 tataaacagc aggatgacac tccatgtagt caaactcaga ggggacaact ctcaccatat
     1621 agctgaatgt acgatgaagt ccatagctat ctccttgaga gaggcagtga aaaggacaga
     1681 agaggtcctc tctacaaaag gggtcttgta ggattgatta ccataataga ttatggggct
     1741 ggtaatctct tcagcgtaaa aaaagcactg gaatacttta atgctgatgt tcagatctca
     1801 gataatggca aaattatact taaatctgac gggataattc tgccaggagt tggtgccttt
     1861 ggtgcaggta taaa
//
LOCUS       DAOTKU010000346         1869 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_49961_length_1869_cov_3.148842, whole genome shotgun sequence.
ACCESSION   DAOTKU010000346 DAOTKU010000000
VERSION     DAOTKU010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49961_length_1869_cov_3.148842"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1603
                     /locus_tag="P5310_07490"
     CDS             <1..1603
                     /locus_tag="P5310_07490"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HRU33238.1"
                     /translation="CQERLVPNYEKIEFGMSEKLIARSLSKALGKDENEIWSLYKEMG
                     DLGLLAQEYIKTKGDVLEILEVYNRLLDIATTAGKGSVEKKTDELAKLFSLASGKEAK
                     YISRIVLGRLRLGIGDPTVLDAFSYAYCGDKSLRPVIERAYNLCSDLGLVGYTLFSKG
                     LDAIKDFKVQVGKPIRMALAERLPTAEDIIAKIGRCSVEPKFDGFRCQIHKDGKDIKI
                     FSRNLEDNTEMFPDLVDGARKQINSEKAIIEGEALAFNEEAQEFYPFQVTVQRKRKYQ
                     IETMMIDLPLKLFAFDLLYLDGEDYTSRPYIERKEKLGKLILPGNVIMVADYIITDDP
                     KELDAFFENAIGSGLEGIVAKRLDAPYQAGARNFNWIKLKRSYKGELTDTVDLVILGY
                     YVGRGARAKFGIGSLLAGVYDPETDTFKTIAKIGSGLTEDEWVKMKNILDEIKVDKKP
                     SRVISVLEPDVWVEPRYVVEVRADEITRSPVHTAGKDEGELGYALRFPRVERFIRVDR
                     VPEDATTVKEIQEMYDMQKKVEVKE"
     gene            1606..>1869
                     /locus_tag="P5310_07495"
     CDS             1606..>1869
                     /locus_tag="P5310_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HRU33239.1"
                     /translation="MASRFLRKDLNSFSPYSVTYYPDFIKLDANESPFELPDEVKEEI
                     KSQFSKLSLRIYPDPSVLELRKSIASFLDIEGLTEENIAVGNGS"
BASE COUNT          621 a          262 c          481 g          505 t
ORIGIN      
        1 atgtcaggag cgcctcgttc ctaactatga gaagatagaa tttggtatga gcgaaaaatt
       61 aattgccagg tctctttcaa aagctcttgg taaagatgaa aatgaaatct ggtctttata
      121 taaagagatg ggagaccttg gacttttagc ccaggagtat ataaaaacga aaggagatgt
      181 attagagatc ctggaagtat ataacaggct gttagatata gccaccaccg ctggaaaggg
      241 ttcagtagaa aagaaaacag atgaattagc caaattattt tcacttgcct caggtaaaga
      301 ggcaaagtat ataagcagaa tcgttctggg aaggttgaga ttagggatag gtgacccaac
      361 agtattggat gccttttctt atgcatactg tggagataaa agtttgagac ctgttattga
      421 gagggcttat aacttatgtt ctgacctggg tctggttggt tatacactgt tcagcaaggg
      481 tttagatgca ataaaggact tcaaggttca ggtggggaaa ccgatcagga tggcactggc
      541 agagagactt ccaactgcag aagatattat agctaagata ggtagatgtt ctgtagaacc
      601 gaaatttgat ggatttagat gtcagattca taaggatggt aaagatataa aaatattttc
      661 caggaacttg gaggataata cagagatgtt tcctgatctt gttgacggtg ctagaaaaca
      721 gataaactca gaaaaggcta taatagaagg ggaagctttg gcttttaatg aagaggctca
      781 agaattctat ccattccagg tgactgtgca gaggaaaaga aaatatcaga tagagactat
      841 gatgatagat ctacctctaa aattatttgc ctttgactta ctctacctgg atggagaaga
      901 ttatacatcc agaccatata tagaaaggaa agagaaactt ggaaagttaa tcttaccggg
      961 gaatgtcatt atggtggcag attatataat tacagatgac cctaaagagc tggatgcttt
     1021 ttttgagaat gccattggta gcgggttaga aggtatagtg gcaaagagat tagatgcccc
     1081 ttatcaggct ggtgccagaa attttaactg gataaaatta aagagaagtt ataaaggtga
     1141 actgaccgat acggtggatc tggttattct cggatattat gtgggaagag gagcaagggc
     1201 aaaattcggt ataggttctc tactggcagg tgtctatgac ccggagactg atacattcaa
     1261 gactatagcc aagataggtt caggactcac cgaggatgaa tgggttaaga tgaagaatat
     1321 tcttgatgag ataaaggtcg ataagaaacc gtccagagtg atctcggttt tggagcctga
     1381 tgtatgggta gaaccaagat atgtggttga ggtgagggca gatgagatta ccaggagtcc
     1441 agtgcataca gccgggaaag atgagggtga attagggtat gcgttgagat tcccaagggt
     1501 agagaggttt ataagggtgg atagggtacc tgaggatgcc actaccgtta aagagattca
     1561 ggagatgtat gatatgcaga aaaaagtaga ggtgaaggag taaaaatggc tagtagattt
     1621 ttaaggaaag atcttaattc tttttctccc tattcagtta cttattatcc agacttcata
     1681 aagcttgatg ctaatgagag tccatttgag ctaccggatg aggtaaagga ggagataaag
     1741 agtcaatttt ccaaattatc actcaggata taccctgacc ccagcgtgtt agaattgagg
     1801 aaatctattg catcattttt agatatagaa ggattaacgg aagaaaatat agctgtaggt
     1861 aacgggtca
//
LOCUS       DAOTKU010000347         1867 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_50052_length_1867_cov_2.592715, whole genome shotgun sequence.
ACCESSION   DAOTKU010000347 DAOTKU010000000
VERSION     DAOTKU010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50052_length_1867_cov_2.592715"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1227
                     /locus_tag="P5310_07500"
     CDS             <1..1227
                     /locus_tag="P5310_07500"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRU33240.1"
                     /translation="LVYCVIPDLWFRYASPRVLRRGNSSRRYVYFTFDDGPHPVYTLQ
                     IINILKKYDVRAIFFVLGKKAEKFPQSIRSIKDSGNFIGNHGYSHRPIWILPPGIAVK
                     EFEKTDRIIFDLIGERASYIRPPWGGVNLGLVRFLNKEDRQVVLWTLDSKDWQRGVSE
                     EAVIKRVVEGVKPGDVILFHDGRWDNISERTVKVLPRIIEGLRDRGYEITGDLGKETY
                     ERSGILRTLVKTIWELWDRLFYIVTDTIKLDDPYMVLSFSINRYKWKPIYLKDGTVIK
                     RGDRFLEIHFLNDIISNIPKTHKSPMGAGKEIKDRLIYSFDRILKYIEENDLNDVKAF
                     HGVTVLYRMLGLDNIDTFDLNIFTRFFVNLYEKLILVIYHPEGFKRLRHRRKLSPKSI
                     WISRNAMRSIVKRYRR"
     gene            complement(1230..1439)
                     /locus_tag="P5310_07505"
     CDS             complement(1230..1439)
                     /locus_tag="P5310_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014956248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HRU33241.1"
                     /translation="MCLAVPALVKEINGDKATVELEGVTLEVSVMFTPEVKPGEYVIV
                     HAGYTIAVMDEKEAEETIEILKEII"
     gene            1490..>1867
                     /locus_tag="P5310_07510"
     CDS             1490..>1867
                     /locus_tag="P5310_07510"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRU33242.1"
                     /translation="MKSKHILFLALLFITLIWGSTFALIKVALDSIGPFYFLAIRFSL
                     ASIVMLIIFRKSFKEHKIHFGYILAGVFLFLGYVFQTVGLKYTSASNAAFITGLSVVI
                     VPFLEVIFMKRKLPGTSILGAISS"
BASE COUNT          576 a          307 c          370 g          614 t
ORIGIN      
        1 ctggtgtatt gtgttatccc ggacttatgg tttagatacg cttctcctag ggttcttcgg
       61 aggggaaact cttccagaag atatgtttac tttacctttg acgacggtcc tcatccagta
      121 tataccttac agataatcaa tatactgaaa aaatatgatg tcagagctat tttctttgtc
      181 ctggggaaaa aggcggagaa atttcctcag agtatcaggt ctataaaaga ctccggtaac
      241 tttataggta atcatggcta ttctcataga cccatatgga tactacctcc aggaattgct
      301 gtgaaggagt ttgaaaagac tgatagaatt atctttgatt taatagggga aagagccagt
      361 tatataagac ctccctgggg tggagttaac cttggacttg taagattcct aaataaagag
      421 gatagacagg ttgtcctctg gacacttgac agtaaagact ggcagagagg ggtaagtgaa
      481 gaggctgtta taaagagagt ggtagaagga gttaaaccgg gagatgtaat cctctttcat
      541 gatggacgct gggataatat aagtgaaaga accgtaaaag ttttacctag gataatagaa
      601 ggtttaaggg atagaggtta tgaaattact ggtgatctgg gcaaagagac gtatgaaaga
      661 tcaggtatat tgagaaccct tgtgaaaaca atctgggaat tatgggatag actattctac
      721 atagtaactg atacgataaa attggatgac ccttatatgg ttctttcatt ttctataaat
      781 aggtataaat ggaaacccat ttatcttaaa gatggaactg taattaaaag gggggacagg
      841 tttttagaga ttcacttctt aaatgacatc ataagcaata taccgaaaac tcacaagtct
      901 cctatgggtg caggtaaaga gataaaagat agactgatat attcgtttga tagaatactt
      961 aaatacatag aagagaatga tttaaatgat gtgaaggcat ttcacggagt taccgttctt
     1021 tacaggatgt tagggttaga caatatcgat acatttgatt taaatatatt taccagattc
     1081 tttgtaaatc tatatgaaaa gttaatcctg gttatctatc atcctgaagg attcaaaaga
     1141 ctaaggcata gaagaaagtt atcccctaag tccatatgga tttctagaaa cgctatgaga
     1201 tctattgtta aaagatacag gagataaact tagattatct cttttaatat ttctattgtc
     1261 tcctctgcct ctttttcatc catcaccgct atggtgtaac cggcatgaac tattacatac
     1321 tctccaggtt ttacttccgg tgtgaacatt acacttacct ccaatgttac tccttctagc
     1381 tctactgtag ccttatctcc atttatttct tttaccaatg caggaacagc aagacacata
     1441 attttctcct ccattataag acttgtattt tatgatatca taatagatta tgaagagtaa
     1501 acatatcctg tttttagccc tattatttat tacattaatc tggggttcca ctttcgcttt
     1561 aataaaagtg gctctggata gtatcggacc tttttatttc ctggcgatta gattctctct
     1621 agccagtatt gttatgctta ttatttttag aaagagtttt aaagaacata agatacattt
     1681 cggatacatc cttgccgggg tttttctctt tttaggttat gtatttcaga cagttggact
     1741 caagtataca tctgcctcta atgcggcatt tattactgga ctcagtgttg taatcgttcc
     1801 attcctggag gtcattttta tgaagaggaa gcttcccggc acatctatac ttggtgctat
     1861 atcttct
//
LOCUS       DAOTKU010000348         1860 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_50308_length_1860_cov_2.511357, whole genome shotgun sequence.
ACCESSION   DAOTKU010000348 DAOTKU010000000
VERSION     DAOTKU010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50308_length_1860_cov_2.511357"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            14..1135
                     /gene="ablA"
                     /locus_tag="P5310_07515"
     CDS             14..1135
                     /gene="ablA"
                     /locus_tag="P5310_07515"
                     /EC_number="5.4.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009171835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050066 - lysine 2,3-aminomutase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0046440 - L-lysine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HRU33243.1"
                     /translation="MIAETADSEDIKRAFGIDKKTWEDWRWQIKNRITTLEELEKILN
                     LTERDKEEIGESLKTLKMSITPYYLSLIDQSRYNDPIRLQSIPSLLELEKTPYEVEDP
                     LKEDSYSPIPGLIHRYPDRALLLVTDSCPTLCRHCTRRRLTEKIERKSTHLDLNKAVE
                     YISSHREIRDIIISGGDPLILSDLMLERIISSIRKIKSVEIIRIGTRAPVTLPQRITP
                     ELCEMLKRYHPVWINTHFNHPKEITEESSRACEMLADAGIPLGNQSVLLKGINDDPDI
                     MKNLVQGLLKIRVRPYYLYQCDMVEGLSHFRTTVEKGVEIIEALQGFTSGLAVPTFVI
                     DAPGMGKIPISPQYVVGREGNQIILRNFEGRISAYTNPG"
     gene            complement(1132..>1860)
                     /locus_tag="P5310_07520"
     CDS             complement(1132..>1860)
                     /locus_tag="P5310_07520"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HRU33244.1"
                     /translation="TYLFDPGWTFKPALDLSGWIDKEVNCLSIVTISREVGSGGDDVA
                     HRLCEVLGYRYFDKELMTEVAVKMGISEEEVIDYSEDSYKLRSFIDNLFRRREPLATI
                     TVKGKNSSGEITHMTEVLDEERALKLIQRTIERLREIGNVVIVGRGSQVILRDAPGVL
                     HIRIVAPVEQRIGRIMLNRSLTRNEARDYMETKDRASSEYLRRFYGVDWSDPSLYHLV
                     INMGRWNTDEAVDIIHTALEKIQS"
BASE COUNT          584 a          414 c          367 g          495 t
ORIGIN      
        1 gaagcagaga aagatgatag cagaaacggc agattcagaa gatataaaaa gagcttttgg
       61 aattgataaa aagacatggg aagattggag atggcagata aaaaacagga tcaccacctt
      121 agaggagtta gagaagatat tgaatttaac tgagagggat aaggaagaga taggggagag
      181 tctgaaaact ctgaagatgt caattacacc ttactatctt tctctaatag accagtccag
      241 gtataacgat ccaataagat tgcaatcaat tccaagtctg ttagagctgg aaaagactcc
      301 ttatgaggta gaagacccat taaaagaaga ttcatattct cctataccag gattaataca
      361 tcgttatcct gatagagccc tcctgctggt aacggactcc tgccctaccc tatgcagaca
      421 ttgcacgagg agaagattaa cagaaaagat tgagaggaaa agtactcact tagatttgaa
      481 taaagcagtt gaatatatct catcacatag agagatcagg gatatcatta tctcaggtgg
      541 agacccgtta atactctcag atcttatgct ggaaaggata atctccagca taagaaaaat
      601 caagagtgtt gagattatca gaatagggac gagagcccca gtcaccctac ctcaaagaat
      661 aactccagaa ctatgcgaga tgctaaagag ataccatcca gtatggataa atacacattt
      721 taaccacccg aaagagatca ctgaagagtc atccagggca tgtgagatgt tagctgatgc
      781 aggcatccct cttggaaatc aatcggtact cttaaagggt ataaatgacg accctgatat
      841 aatgaaaaat ttggtccagg gattacttaa gataagggta agaccctatt atttatacca
      901 gtgtgacatg gtggagggtc tttctcattt cagaaccact gtcgaaaaag gggtagagat
      961 tatagaggct ctccagggat ttacctccgg gctggcagtc ccaacgttcg tcatagatgc
     1021 ccctggaatg ggcaaaatcc cgataagccc ccagtatgtg gttgggcgag aggggaatca
     1081 gataatactc agaaacttcg agggaagaat ctccgcctac acgaacccgg gctaactctg
     1141 gatcttctcc agtgcggtat gtataatatc caccgcttcg tctgtattcc atctacccat
     1201 atttattacc agatggtaca gcgatgggtc actccaatcc accccataaa acctgcggag
     1261 atactctgaa gatgccctat ctttagtttc catataatct ctcgcctcgt ttcttgtcag
     1321 actgcgattc agcattatac gacctattct ctgttctaca ggggctacta tccttatatg
     1381 gagtactcca ggagcatctc taagtataac ctgactaccc cttcccacta taacaacatt
     1441 acctatctct ctcagtctct ctatagttct ctggatgagt tttaaagccc tctcttcgtc
     1501 cagaacctct gtcatatggg ttatctcacc tgaagagttc ttgcctttta ccgtaatagt
     1561 tgccagtggt tcccttcgtc taaagaggtt atctataaaa gacctgagtt tatagctatc
     1621 ctcagaataa tctataacct cctcttcaga tatccccatc tttactgcaa cttctgtcat
     1681 aagctccttg tcaaaatagc gatatcccag tacttcacat aacctgtgtg ccacatcatc
     1741 tccaccacta cctacttctc tagatattgt tactattgac aaacaattca cctccttatc
     1801 tatccagcca gatagatcca aagctggttt aaaagtccag ccaggatcaa agagataagt
//
LOCUS       DAOTKU010000349         1859 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_50343_length_1859_cov_2.449557, whole genome shotgun sequence.
ACCESSION   DAOTKU010000349 DAOTKU010000000
VERSION     DAOTKU010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50343_length_1859_cov_2.449557"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="P5310_07525"
     CDS             complement(<1..251)
                     /locus_tag="P5310_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU33245.1"
                     /translation="MFFIDTVGTRGENPVAKILNFIRDVGGFISRIKVSMEESFISAY
                     MFLERQSHLVQYSRNLELQIARLKTENNVLTNQAEELKRL"
     gene            complement(296..1324)
                     /locus_tag="P5310_07530"
     CDS             complement(296..1324)
                     /locus_tag="P5310_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HRU33246.1"
                     /translation="MNILSKDIGIDLGTSNTVVFLKGRGIVLREPTVIAVSIGSYDIL
                     AVGAEAKRMLGRTPSDIIAIRPMKDGVIANFEITQTMLEYFISKSQGRRGLLRPRVVI
                     GIPSGSTEVERRAVHEAAMKAGARDVYLVDEPMAAAIGAGLPVAEPRGSMIVDMGGGT
                     TEIAVISLGGIVTSKSIRVAGDEMDDAIIQHIKKEYNLLIGERTAEEVKMKIGSAYPL
                     DEEITMEIKGRDLLSGLPKTIEIDSEEIRNALQPVVAAIIDAVKSALEATPPELASDI
                     VERGIILAGGGALLKGIDRIISQNTEMPVFVADDPLSCVALGTGKMLEEVAILKRVTS
                     YRGKGGRL"
     gene            complement(1321..>1859)
                     /locus_tag="P5310_07535"
     CDS             complement(1321..>1859)
                     /locus_tag="P5310_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HRU33247.1"
                     /translation="LLNRLGIEFEVIPSNITEPFPSSEEDPVSFARNLSFLKAYNVYQ
                     LIEEGVILGVDTIVEINNLILGKPKDREEARYMLRLLSGNTHRVISGFTIIDKKREKW
                     VSDVAITAVKFRRLKYDDIENYLDTEDYIDKAGAYAIQGFGSTLVEYIRGCYYNVVGF
                     PIERIMGALKLFLMEEKR"
BASE COUNT          507 a          481 c          268 g          603 t
ORIGIN      
        1 agtcttttca attcttctgc ctgattagta agcacattat tctctgtttt caatcttgct
       61 atctgaagtt ccaggtttct actatactgg acaagatggg attgtctctc cagaaacata
      121 tacgcagaaa taaaagattc ctccatagat acctttattc tggagataaa tccaccaaca
      181 tcccttataa aattcaaaat cttagcgaca ggattttcgc ccctggtacc aaccgtatct
      241 atgaaaaaca taaaaagggc tagagataat actataagag ctataatgat attcttcaga
      301 gtctccctcc ttttcctcta taagaggtaa cccttttcaa gatcgctacc tcttccagca
      361 ttttaccggt gccaagagct acacaggaca atggatcatc agccacaaat accggcatct
      421 ctgtgttttg agagataatt ctatctatcc cttttaacag tgctccacct cctgccagga
      481 tgatgcctct ctctactata tcactcgcca gttcaggtgg agtggcttct aatgccgatt
      541 ttacagcgtc aataattgca gcaaccaccg gttgcaatgc gtttcttatc tcctcactat
      601 caatttctat cgtctttgga agaccgctta atagatctct cccctttatc tccatagtaa
      661 tctcttcatc caatggatag gcagaaccta tcttcatctt aacctcttcc gcggtccttt
      721 ctcctataag tagattatat tcttttttta tatgctggat aattgcatcg tccatctcat
      781 caccagccac tctgatagac ttggaagtca ctataccacc cagcgatatg accgctatct
      841 ctgttgtacc tccacccata tctactatca tactgcctct aggctcagct accggtaaac
      901 cagccccaat agcggctgcc attggctcgt ctaccaggta aacgtctctg gctccagctt
      961 tcatagctgc ctcatgaacc gcccttctct ctacctcggt gctaccactg ggtataccta
     1021 tcaccactct tggtctaagg agacctctcc ttccctgaga tttacttata aagtattcca
     1081 gcatggtctg agttatttca aaattagcta taacaccgtc cttcattggt cttatagcta
     1141 taatatcact tggggttctt cccagcatcc tctttgcctc tgcaccaact gccaggatat
     1201 cataactacc tatactgaca gctataacag tgggctctct taatacaata cctcttcctt
     1261 ttaaaaatac caccgtgttt gatgtgccca aatctattcc tatatcttta gaaagaatgt
     1321 tcatcttttc tcctccatta agaaaagttt caatgctccc ataattctct ctatcggaaa
     1381 tccaaccaca ttataataac atcctcttat atattctacc agagtgctac caaaaccctg
     1441 aatggcataa gctccagctt tatctatgta atcctcggta tccagataat tttcaatatc
     1501 atcatatttt aatctgcgga actttaccgc agtaattgca acatctgaga cccacttttc
     1561 tcttttctta tctataatgg taaaccctga gattactcta tgggtattac cggagagtaa
     1621 tctcaacata tacctcgcct cttctctgtc ctttggtttc cccagtatta aattatttat
     1681 ctctacaatt gtatcaaccc ctaaaatcac accttcttct atcaactgat aaacattata
     1741 agcttttaga aaggagagat tcctggcaaa actgacagga tcttcttctg aagatggaaa
     1801 gggttctgtt atattacttg gaatgacttc aaactctatc ccgagcctat ttaataatt
//
LOCUS       DAOTKU010000350         1845 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_50871_length_1845_cov_2.643017, whole genome shotgun sequence.
ACCESSION   DAOTKU010000350 DAOTKU010000000
VERSION     DAOTKU010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50871_length_1845_cov_2.643017"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..785
                     /gene="dapA"
                     /locus_tag="P5310_07540"
     CDS             <1..785
                     /gene="dapA"
                     /locus_tag="P5310_07540"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HRU33248.1"
                     /translation="EGSDGILVAGTTGESPTLSEEEKIELFREIKNVIKDKASIIAGT
                     GNYNTQESIALTKAAEEVGVDGILLVAPYYNKPPQDGLYAHFKAVANATSLPVILYNI
                     PSRTGVNILPPTVIRLAKEVKNFIGVKEASQSTDQVVEILRGIDRDDFVIYSGDDSFT
                     MPILSVGGYGVISVASHIVGREIKEMMNSFVFGDVKKAVQINKRLFPLFKSLFVTTNP
                     IPLKEAMRLTGFDIGPPRLPLVRAGEKEIGVIRDSLKQLGLI"
     gene            795..>1845
                     /locus_tag="P5310_07545"
     CDS             795..>1845
                     /locus_tag="P5310_07545"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HRU33249.1"
                     /translation="MSEKLKLIPLGGLGEIGKNMLVVEYGEVIAIIDTGLMFPTDEMP
                     GVDYVIPDISYIEANKNKVKGIILTHCHEDHVGALPYVLKTVRVPIIGTRLTLGMIMS
                     RLKDFGLSNDVKLVEVQERSRIGIGPLSFDFFVVNHSVPGNLGVAIRSPVGIIVHTGD
                     FKFDYTPIDGKYTDFSTLASLGEEGVKFLLSDSTNAERPGYTPSERIVGDTLDSIVRG
                     ATGRVFITTFASNVHRIQQVFNIAYKYKRFVAVIGKSMNNTVSVASRLGYLDIPPNTL
                     IKDQSLSNFKKNQLIILTSGSQGEPMSALTRLAFQEYKSITIGSGDTVVIAATPVPGN
                     EKMVARTINALFSRGA"
BASE COUNT          556 a          324 c          452 g          513 t
ORIGIN      
        1 aagaaggttc tgacgggatc ttagtcgctg gaactactgg agaatctcct actctgtcgg
       61 aggaagaaaa gattgagctc tttagagaaa tcaaaaatgt tataaaggat aaagccagca
      121 taatagccgg cacaggcaat tataatactc aggagagtat agctctcact aaagcggcag
      181 aagaagttgg ggtggatggt atattacttg ttgcgccata ctataataag ccaccgcagg
      241 atggtctgta tgctcatttc aaggctgtag caaatgccac atcgcttcct gtaatcctgt
      301 ataacatacc atccagaact ggtgtgaata ttttacctcc aactgttatc cgtctggcta
      361 aagaggtaaa gaactttatc ggggtaaaag aagccagcca gagtacagac caggtggtag
      421 agatactgag gggtatagat agggatgatt ttgtcatata cagtggagat gattctttta
      481 caatgcccat actcagtgtt ggtggatatg gggttataag tgtggcaagt cacatagtcg
      541 ggagagagat aaaagagatg atgaatagct ttgtctttgg agatgttaag aaggctgttc
      601 agattaataa gaggctattc cctctattta agtcgctctt tgttaccact aaccctatac
      661 ctctgaagga ggcgatgagg cttacaggct ttgatatcgg tccacctaga ttacctctgg
      721 taagggctgg tgaaaaagag attggtgtga taagggattc attaaaacaa cttgggttaa
      781 tttagaaagg atatatgtcg gaaaaattaa aacttattcc tctaggggga ttgggagaaa
      841 tagggaagaa tatgctggta gtggagtatg gcgaggtaat tgccatcatc gataccgggc
      901 ttatgtttcc caccgatgag atgccagggg tagactatgt gatacctgac atctcatata
      961 tagaagccaa caaaaataag gtgaagggga taattcttac ccattgtcac gaagaccatg
     1021 tgggtgcgtt accatatgta cttaagaccg ttcgggtccc gataatagga acaagattaa
     1081 ctcttgggat gataatgtcc aggttaaaag actttggtct aagtaatgat gttaagcttg
     1141 tagaagttca ggagaggagt cgtataggga ttggtcccct ctcttttgac ttttttgtgg
     1201 ttaaccatag tgttcctggt aacttagggg tagcaatacg ttctccggtt ggaattatcg
     1261 tgcataccgg tgactttaaa tttgactaca cacctataga tgggaaatat acagactttt
     1321 ctacactggc ttcacttgga gaagaagggg taaaattttt actatcagac tctaccaatg
     1381 cagaaagacc cggctataca ccttccgaga ggatagttgg tgacaccctt gattcgatag
     1441 tgagaggtgc tactgggagg gtattcataa cgacatttgc cagtaacgtg catcgtattc
     1501 aacaggtatt caatatcgct tataagtata agagatttgt agcggtaata ggaaagagta
     1561 tgaataatac ggttagcgtg gcaagccgtc ttggatatct cgatataccc cccaatactc
     1621 tgataaaaga tcaatctctg tcgaacttta aaaagaatca gcttatcata ttaaccagtg
     1681 gttctcaggg agaaccgatg tctgctctaa ctcgactcgc ctttcaggaa tataaatcta
     1741 tcaccattgg ttctggagac acagtggtaa tagcagctac tcctgttcct ggaaatgaga
     1801 agatggttgc caggactata aacgcattat tttccagggg ggcgg
//
LOCUS       DAOTKU010000351         1844 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_50898_length_1844_cov_3.266629, whole genome shotgun sequence.
ACCESSION   DAOTKU010000351 DAOTKU010000000
VERSION     DAOTKU010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50898_length_1844_cov_3.266629"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1004)
                     /locus_tag="P5310_07550"
     CDS             complement(<1..1004)
                     /locus_tag="P5310_07550"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRU33250.1"
                     /translation="MKGFKSFTTLILCFVLVIGVITSAFPQQKNPLGQYATLTEYEKA
                     TGKKVTKFNEAPVLAELVKQGKLPPVEKRLPDEPAVVEPVEEIGQYGGTWRRAALSPT
                     DTQIQERLGYEPLVRWARDGKTLIPNLCTSWKIGEGGRVYTFYLRKGVKWSDGEPFTA
                     DDIMFWYEDVLLNKELTPTFPKWLTVEGSPAKVEKVDTYTVRFRFPRPYSLFLDYLAG
                     PEGPGPNLFNYPRHYMRQYHPKYTSKEKLDTMVKEAGLQNWYQLFGNKAQPYTNKELP
                     TLRPWVLRSDPTAIRIIAERNPYYWKVDPAGNQLPYIDKIAFDMVQDIEMINMKSVSG
                     "
     gene            complement(1042..>1844)
                     /locus_tag="P5310_07555"
     CDS             complement(1042..>1844)
                     /locus_tag="P5310_07555"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRU33251.1"
                     /translation="KDYTDMLNSEKLDAISICTPHNTHRDIALNVMDFRIPILLEKPA
                     ATSREEIKELIEKSEALGVKIMVAHSLRFSETFRTAKNSLSSGDIGDILFIIGKYISF
                     KDYSLYPKWKTTKREAWGGTLFRDGIHIIDSILWFSGKEIVSADGMPLNLYFDTEVED
                     TFLGTIKFQDGSVAQLSMSSITRGFEQIGVEVYGTKGSLKVDNNELVIYKDEKPPRYI
                     KPAIGPSDYWKNQIDHFVDCVTNNKEPLVTLKEASKTMEAIFLLYERE"
BASE COUNT          478 a          429 c          324 g          613 t
ORIGIN      
        1 tctccagaca ctgacttcat atttatcatt tcaatatcct ggaccatatc aaaggctatc
       61 ttgtctatat atggcaactg gtttcctgca ggatcgacct tccagtagta cggatttctc
      121 tctgctatta tccttattgc cgttgggtct gatctgagta cccagggtct tagagttggc
      181 aattctttat tggtgtatgg ttgtgcctta ttaccaaaca gctgatacca gttttggaga
      241 ccagcttctt ttaccatggt gtcaagcttt tctttggatg tatacttggg atgatactgt
      301 ctcatatagt gcctgggata gttaaagagg tttggtccag gtccttcagg tcctgcaaga
      361 taatccagga aaagagaata aggtcttggg aatctaaacc taacagtata ggtgtctact
      421 ttctcaactt ttgcaggaga accctctact gttagccatt taggaaaagt gggagtaagt
      481 tctttattca atagaacatc ttcataccag aacataatat catctgcagt gaatggctct
      541 ccgtctgacc actttacccc tttcctcaga taaaatgtat aaactcttcc tccctctcct
      601 atcttccagg aggtacagag attaggtatc agagtcttcc catctcttgc ccatcttacc
      661 agaggttcat aaccaagtcg ctcctgtatc tgtgtgtctg tagggcttaa tgctgctctt
      721 ctccatgttc caccatactg acctatctcc tctacaggct ccactacagc aggttcatcg
      781 ggtaatcttt tctctaccgg tgggagctta ccctgtttta caagctcagc taatacaggt
      841 gcttcattga actttgttac cttcttacca gttgccttct catactctgt tagcgtagca
      901 tactgcccca gaggattctt ctgctgagga aaagccgatg taatcacccc tatgaccaaa
      961 acaaaacaca aaattaaagt tgtaaagctc ttaaacccct tcattttaaa cacctcctga
     1021 ttgaataata aaatttttcg attattccct ctcatagagg aggaatatcg cctccatggt
     1081 tttacttgcc tctttcaaag tcaccagagg ctccttattg tttgttacac aatccacaaa
     1141 gtggtcaatc tggtttttcc agtagtcgga tggacctata gctggtttta tatacctggg
     1201 aggtttctca tctttataga tgacgagttc attattatca acctttagag aacctttcgt
     1261 cccatagacc tctaccccta tctgctcaaa acccctggtt atactgctca tggacagctg
     1321 agccacactg ccatcctgaa actttatcgt ccccaaaaag gtatcctcca cctcagtatc
     1381 aaagtataga ttcagaggca taccatctgc ggaaactatc tctttcccgg aaaaccatag
     1441 tatagaatct attatatgga ttccatctct gaagagagtt cctccccagg cttctctttt
     1501 tgttgtcttc cacttcggat agagggagta atctttaaaa gatatatatt tgcctattat
     1561 aaacagaata tctcctatat ccccactgga taagctattc ttagctgttc tgaatgtctc
     1621 gctgaatcta agagaatggg caaccataat tttaacacca agagcttctg atttttctat
     1681 caactctttt atctcttctc tgcttgttgc ggcaggcttt tctaaaagta taggtatcct
     1741 aaaatccata acattcagag ctatatctct atgtgtgttg tgtggggtgc atatacttat
     1801 ggcatcaagt ttttctgaat ttagcatatc tgtataatct ttat
//
LOCUS       DAOTKU010000352         1844 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_50899_length_1844_cov_3.229178, whole genome shotgun sequence.
ACCESSION   DAOTKU010000352 DAOTKU010000000
VERSION     DAOTKU010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50899_length_1844_cov_3.229178"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="P5310_07560"
     CDS             complement(<1..392)
                     /locus_tag="P5310_07560"
                     /inference="COORDINATES: protein motif:HMM:NF014269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HRU33252.1"
                     /translation="MSDENRIPEILPILPLRETVIFPSMVAPVVVGRERSIRLIDEVA
                     VGSKMFGVVAQKDSQAEDPTPEQIYNIGTLCTIIRMLKFPDGTTRLLLQGISRIRINQ
                     ILETEPYLKAQVAPIQEVYELTIEIQGL"
     gene            complement(389..814)
                     /locus_tag="P5310_07565"
     CDS             complement(389..814)
                     /locus_tag="P5310_07565"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HRU33253.1"
                     /translation="MSNWRPMDFSDDFWRMDREVQQFIECIFGSYSWKPSVDVYETDK
                     EIIVLIEIAGVNKEDIQIFIKGNLVTVRGTRREPDINRKEAYHQMEINYGTFERVISL
                     PVVVREEDAKASFQNGILKIVFPKANRVTNISIEEEEGL"
     gene            complement(820..1323)
                     /locus_tag="P5310_07570"
     CDS             complement(820..1323)
                     /locus_tag="P5310_07570"
                     /inference="COORDINATES: protein motif:HMM:NF040826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCAM0308 family protein"
                     /protein_id="HRU33254.1"
                     /translation="MSMKRRQIRETTEHLDDPYIDGVEYHDPTRCPSCGVVYNKKRWR
                     FMDVKEIEYDKENDHIDIAKCPACRKIEDHYPMGIVELSGKFVDEHKEEILNLIKNEE
                     GREMEKNPLGRLMKIEDRDGKLYIETTNEALALRIGRALFKAFKGEVEYKFSETQKLV
                     RVMWRRD"
     gene            complement(1320..>1844)
                     /locus_tag="P5310_07575"
     CDS             complement(1320..>1844)
                     /locus_tag="P5310_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU33255.1"
                     /translation="HFGITTLYVTHDQIEAISLGHRLAIMRRGKIVQQGTFEDIYNRP
                     YNVFVAGFLGNPPLNLIKGEVIDNVFYGGGIRFPTTGLLGEVIGGIKVEDITSGGDFK
                     GIVEVSEPNPSEKSQIVTVRIKQNFRIKLRKSLDYILEAGEIINFSVKRMYFFDPVTE
                     LFIKSIENKEAVSI"
BASE COUNT          503 a          454 c          257 g          630 t
ORIGIN      
        1 ataagaccct gaatctctat ggttaactca taaacttcct gtatcggtgc tacctgtgcc
       61 ttaagatatg gttcggtctc aagtatctga tttatcctta ttcttgatat cccctgaagt
      121 aaaagtcttg ttgttccatc agggaatttc agcatacgga ttattgtaca tagtgtccct
      181 atgttatata tctgctctgg tgtagggtct tctgcctgtg aatctttctg ggctactaca
      241 ccgaacatct tactcccaac tgcaacttca tcgataagtc ttattgacct ctctctccca
      301 acaactacag gtgcaaccat tgaggggaag ataaccgttt ctcttaatgg gagtatcgga
      361 aggatttctg gtattctgtt ttcatcactc ataagccttc ctcctcttct attgaaatat
      421 tggttacccg attagcttta ggaaacacaa tcttaagaat cccgttctga aaagaagctt
      481 tagcatcctc ttcccttaca actacaggga gagagattac cctctcaaat gttccatagt
      541 ttatctccat ctgatgataa gcctcttttc tatttatatc aggttctctt ctggttcctc
      601 ttactgtaac caggtttccc tttataaata tctgtatatc ctctttgttt acccccgcta
      661 tctctatcag tactataatc tctttatctg tctcatacac gtctaccgat ggcttccacg
      721 agtaagaacc aaatatacac tctataaatt gttgaacctc cctatccatt ctccaaaaat
      781 catcagaaaa gtccataggt ctccaattag acatataaac taatccctcc tccacatgac
      841 acgaactaac ttttgagtct cactgaattt atactccact tcacccttaa aggctttaaa
      901 taatgcccta ccaattctga gagcaagagc ttcgttggta gtctcaatat aaagtttacc
      961 gtccctgtct tcgatcttca tcagtctacc cagcgggttc ttctccatct ctctcccctc
     1021 ttcattcttt ataagattaa gaatctcttc tttatgctca tcaacaaatt taccagatag
     1081 ctcgacaatc cccataggat aatggtcttc tatctttctg catgctggac atttagcaat
     1141 atctatatga tcattctctt tgtcgtactc tatctctttt acatccataa aacgccaccg
     1201 ttttttatta tatacaactc cacaagatgg acatctagta gggtcatgat attcaactcc
     1261 atctatatat ggatcatcca gatgttcagt ggtctctctt atttgtctac gtttcatact
     1321 catatactta cagcctcctt attctctata ctctttataa ataactctgt tacaggatca
     1381 aaaaagtaca tccttttcac ggagaaattt ataatttcac cagcttccaa tatataatcc
     1441 agactctttc taagctttat acggaagttt tgttttattc taaccgttac tatctgagac
     1501 ttctcagaag gatttggttc acttacctct acaatacctt taaaatcacc tcctgaagtt
     1561 atatcttcta cctttatccc ccctatcacc tctccaagga gaccagtggt ggggaatctt
     1621 atcccacctc cataaaaaac attgtctata acctctcctt ttataaggtt taagggggga
     1681 ttccccagaa aaccagcgac aaatacatta taaggtctat tataaatatc ctcaaaagta
     1741 ccctgttgta ctatctttcc acgtctcatt atagctaacc tgtgccccag ggagatcgcc
     1801 tctatctgat cgtgagtaac ataaagggtg gttataccaa aatg
//
LOCUS       DAOTKU010000353         1830 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_51480_length_1830_cov_2.782535, whole genome shotgun sequence.
ACCESSION   DAOTKU010000353 DAOTKU010000000
VERSION     DAOTKU010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51480_length_1830_cov_2.782535"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1136
                     /locus_tag="P5310_07580"
     CDS             <1..1136
                     /locus_tag="P5310_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004630438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HRU33256.1"
                     /translation="RIKPGSYIAIAAGSRGIRNIHRIVKATVEVVKEFGGRPFIVPAM
                     GSHGGATAEGQKELLESYGITEEYTGAPIRSSMEVVELPSNGLENKVYMDKLAYSADG
                     TIVINRIKPHTDFHGDIESGLLKMCVIGLGKHKQAIEIHRFGTYGLKYLIPPTAKRII
                     EYGNIILGIGIVENAYDETMVISAAKPEDFEREDKRLLDIARGNMPRLPVDKLDLLIV
                     DEMGKDISGAGMDTNIIGRIYIDGETEPEKPKITRIVVTDLTEKAHGNAIGIGLADLT
                     TRRLVNKIDFNATYQNAVTSTFLHRGKIPVIAEDSKTAIEWALMTCGPIDIPNARIIR
                     IKNTLSLNELYISRSVLEEIKDRIEIIGDFTDILDEEGELIKF"
     gene            1149..1346
                     /locus_tag="P5310_07585"
     CDS             1149..1346
                     /locus_tag="P5310_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6485 family protein"
                     /protein_id="HRU33257.1"
                     /translation="MRECNINRNLKSCNCSYEPCSRKGICCECVAYHRSNGELPACYF
                     PNEVERTYDRSIERFISLYKR"
     gene            1348..>1830
                     /locus_tag="P5310_07590"
     CDS             1348..>1830
                     /locus_tag="P5310_07590"
                     /inference="COORDINATES: protein motif:HMM:NF018682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HRU33258.1"
                     /translation="MPKLLIIADDLTGALDTGIQFSKIGVDTVVSINPGELVSDTDVI
                     VADTESRHLLPETAYERVRRVLSITGEVGYIYKKTDSTLRGNTGAEFKAIIDETSHPV
                     IFIPAFPEMGRTTKNGIQYINGVPLGESPFASDPVNPGRKSYIPDIIKEQADLDCVVV
                     T"
BASE COUNT          619 a          288 c          463 g          460 t
ORIGIN      
        1 taaggataaa acctggttca tatatagcca tcgctgctgg aagtagaggg ataagaaata
       61 tacacaggat tgtcaaggct actgtggagg tagtcaaaga gtttgggggg agaccattta
      121 ttgttcccgc tatgggtagt catggaggtg caaccgctga agggcaaaaa gaattactgg
      181 agagttatgg tataacagag gagtatacgg gggctcctat caggtcatct atggaggtgg
      241 tggagctccc atcgaatggg ctggagaata aagtttatat ggataaactc gcctattctg
      301 cagatgggac tatcgttata aacaggataa agccacacac agactttcac ggggatatag
      361 agagtggtct tttgaaaatg tgcgtgatag ggcttggaaa gcataaacag gctatagaga
      421 tacatagatt cgggacttat ggcttgaaat atttaatccc acccacagcg aaaagaatta
      481 tagaatacgg gaatattata cttggtatag gtatagtgga gaatgcatat gatgaaacca
      541 tggtcatctc tgcggcaaaa cctgaagatt ttgagagaga agataaaagg ctgcttgata
      601 tagcgagggg aaatatgccc cgcctgccgg ttgataaatt ggatctatta atagtggatg
      661 agatgggtaa agatataagc ggtgctggta tggataccaa tattattgga aggatatata
      721 tcgatggaga gacggaacct gaaaaaccaa agatcacaag aatagttgtc accgacctta
      781 cggagaaggc acatggtaat gctataggca taggtttggc tgatctcaca accagaagat
      841 tggtcaataa gatagatttc aatgctacgt atcaaaatgc tgttacaagc acattcttac
      901 atagggggaa gataccggta atagctgaag actctaaaac agctattgaa tgggctctaa
      961 tgacctgtgg acccatagat ataccgaatg cgaggataat aaggataaaa aatactctga
     1021 gtctgaacga attatacata tccagaagtg tcctggaaga gataaaggat agaattgaaa
     1081 tcattggaga ctttacagat atacttgacg aagaaggaga acttataaaa ttttagcagg
     1141 aggattagat gagagagtgt aatattaata gaaatctaaa gagctgtaac tgttcctatg
     1201 aaccctgttc taggaaaggt atctgctgtg aatgtgtagc gtatcacagg agtaatgggg
     1261 aactaccggc ctgttacttc cccaatgaag tggagagaac atacgatcgt tccatagaga
     1321 ggttcatatc tctatacaaa agataatatg cccaaactcc ttataatagc tgatgacctt
     1381 acaggagctt tagatacagg tatacagttt tcaaagatag gggtagacac agtagtatct
     1441 attaatccag gagaactggt cagtgatacc gatgtgattg tggcagatac cgaaagcagg
     1501 catctcttgc cggagactgc gtatgaaagg gtcaggaggg tattatctat aacaggagag
     1561 gtgggttata tatacaaaaa aacagactct acattgagag ggaatacagg tgcagagttt
     1621 aaagcaataa tagatgaaac atcacaccct gttatcttta tcccggcatt tcctgagatg
     1681 ggaaggacaa caaagaatgg catccagtat ataaatgggg ttccattagg ggagagccca
     1741 tttgcttccg atccggtaaa tccaggcagg aaaagttata tccctgatat cataaaggag
     1801 caggcagatt tagattgtgt tgtggtaact
//
LOCUS       DAOTKU010000354         1825 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_51691_length_1825_cov_2.440678, whole genome shotgun sequence.
ACCESSION   DAOTKU010000354 DAOTKU010000000
VERSION     DAOTKU010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51691_length_1825_cov_2.440678"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(698..1078)
                     /gene="dcd"
                     /locus_tag="P5310_07595"
                     /pseudo
     CDS             complement(698..1078)
                     /gene="dcd"
                     /locus_tag="P5310_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823864.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
     gene            complement(1083..>1825)
                     /gene="nrdD"
                     /locus_tag="P5310_07600"
     CDS             complement(1083..>1825)
                     /gene="nrdD"
                     /locus_tag="P5310_07600"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012544736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HRU33259.1"
                     /translation="LYPYSKFYLREVKRRFGEYWKNHFSTIGLIGMNESCLNLLSVDI
                     GTPEGRNFSLKVLDFMRNKLVEFQEETGNIYNLEATPGEGTSYRLARIDKNKYPDIIC
                     ANEDDYRERGAEPFYTNSTQLPVNYTDDIFYALDLQDEIQTMYTGGTVFHIFVGERID
                     EPENVKNLIRKICEIYRLPYITITPTFSICPSHGYIAGEHQLCPQCGEETEIYSRVVG
                     YLRPVKQWNRGKREEFKLRKTFSVIRGG"
BASE COUNT          487 a          404 c          276 g          658 t
ORIGIN      
        1 tgacataata attacctttt ttatccttat aggtactgcc ttctgcaagc catagcccaa
       61 aaaaactgag ccagtcatct gttcttatcc ttaaaggtgg caagaccaga ggttctaatt
      121 tatgtcctgt ctttttccat aaacataccc tgatactatg tccacctgtg atttttctgc
      181 tttctttcct ctcaagaagc tcttcttttt gaagtttatg gaggtgataa aggaatgtcc
      241 ttctggaaat acctgctgaa cataattcgt aaagagtttc tgtggaaaca tattccccat
      301 ttagctgatt aagaatagta tttcttatct ctgctcctct tttttcaatc acatccaatt
      361 caataggagg tagctcaaag tattcctttt caggggcaag ccagatacaa tccctcttta
      421 attcatagtt atatcttcta tatatctctt tcgcaggaat aaactcaaaa taatctttat
      481 atctattttt agcataaatt agatgggcag gagtaactac caagtcgagg tttcttcctt
      541 taatctgaat catctcaccc ttaaatctga acttttgcat cttttcaatc ttttgatact
      601 ctaaaactcc atctctattt atagtggcta ctacttcatt ccctttcagt tcttcaaatt
      661 tcctccaccc ttcccctgtt aaaatctctg tttctttgtc ataacagggc tcaagagggg
      721 ttacatttac tataattcca catctggcat atgtagattt ccctatgcat ataactatta
      781 cgttcctcgg tatcctgaaa tattcaacag acttagccag ggcaaaggag ttgggtggta
      841 ttatgcatat atcagattct acatcaacaa atgacttgct gtcgaagttt ttagggtcaa
      901 cgatggtact gtttatattc gtgaatatct tgaatttatt atctaccctt acatcgtatc
      961 catatgaaga gagtccatac gatatcttcc cattacctat aagttcaggg cagaatggtt
     1021 ctatcatttt aaactcctct gccatctttt ttatccatct gtctgacttt actcccattc
     1081 ttttagcctc ctcgtataac agaaaaagtt tttcttagct tgaattcttc ccgttttccc
     1141 ctattccatt gctttacagg tcttaaatat ccaactactc gtgaatatat ctctgtctcc
     1201 tctccgcatt gtgggcataa ttgatgctct ccagctatat acccatggct tggacatata
     1261 ctgaacgtag gagttattgt tatatagggt aatctataaa tttcacagat ttttcttatc
     1321 agattcttca cattctcggg ctcatctatc ctttccccaa caaatatatg gaagaccgtc
     1381 cctccggtat acatcgtctg tatctcgtct tgaaggtcaa gggcatagaa gatatcgtca
     1441 gtataattga caggtaactg ggtagaattg gtatagaacg gttctgcacc cctctctcta
     1501 taatcatcct cattggcaca tattatatca ggatatttat tcttatctat tcttgcaagt
     1561 ctgtatgatg ttccttctcc cggggtagct tcgagattgt atatattacc cgtctcttcc
     1621 tggaactcta cgagtttatt cctcataaag tcaagtactt tcaggctgaa gtttctgccc
     1681 tcaggagttc ctatatctac gctgagaaga tttaaacagg attcattcat acctatcagt
     1741 cctatggttg aaaagtgatt cttccaatat tctccaaatc ttctctttac ctctctcaga
     1801 tagaacttag aatagggata taact
//
LOCUS       DAOTKU010000355         1819 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_51945_length_1819_cov_3.806689, whole genome shotgun sequence.
ACCESSION   DAOTKU010000355 DAOTKU010000000
VERSION     DAOTKU010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51945_length_1819_cov_3.806689"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="P5310_07605"
     CDS             complement(<1..228)
                     /locus_tag="P5310_07605"
                     /inference="COORDINATES: protein motif:HMM:NF037181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HRU33260.1"
                     /translation="MSKTGKLYRCTECGYTTSHWLGRCPNCSSWNTIEEVMVEKEPKR
                     SQIKVASSKPLTLTEIRATSIERITTDYGETD"
     gene            complement(225..1598)
                     /gene="lysS"
                     /locus_tag="P5310_07610"
     CDS             complement(225..1598)
                     /gene="lysS"
                     /locus_tag="P5310_07610"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HRU33261.1"
                     /translation="MDFTPTHSIDELKSNFDLTIEQTVRIAGRITAIRRHGRSMFLDI
                     RNLKDRIQLYGKADDLGDSYEELIECHVGDFIGVEGVTFKTHTGEPSVRIHKFYILTR
                     ASRPLPEKWHGITDVETRYRRRYLDLIANENSRKIFLLRARMIKEIRKFLEDMGFIEV
                     ETPILHTVPGGGAARPFITHHNALNIDLYLRIALELYLKRLIVAGYEKVFEINRCFRN
                     EGISTVHNPEFTMMELYQAYADYNTMMSLTEELIVHLASTLFGKPIIYRDNEEIDISP
                     PWDRRELLDIVIEYTGVNPLDLTREDLLEYASKLEIKVTTEESLWAKVLMGLFDEEIQ
                     PKLKRPTFITGYPVAVSPLAKARENDPRFTDRFELFIGGLEVANAFSELNNPVEQRER
                     FEAQLREKMMGEEETHPMDEDFIEALEYGMPPTGGLGIGIDRLTMLFANVDSIREVIL
                     FPLQRPE"
     gene            complement(1605..>1819)
                     /locus_tag="P5310_07615"
     CDS             complement(1605..>1819)
                     /locus_tag="P5310_07615"
                     /inference="COORDINATES: protein motif:HMM:NF013440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HRU33262.1"
                     /translation="IGCRVKLLNLIDNSINEYHIVSSPEVNIKENKISDESPVGKAII
                     GATRGDIIKVKIPRGEIEFKVLDIRR"
BASE COUNT          453 a          416 c          302 g          648 t
ORIGIN      
        1 atctgtttct ccataatcgg tggttatcct ctctatagag gtagccctta tctctgtaag
       61 tgttaatggc ttgctggacg ctactttaat ttgagacctt ttaggttcct tctctaccat
      121 aacctcttct atcgtgttcc aggagctgca gttagggcac cgaccaagcc agtgagatgt
      181 agtataacca cattctgtac acctgtacag tttaccggtc ttactcattc tggtctctgt
      241 aatgggaaca gaatcacctc tcttatagaa tctacattgg caaagagcat tgtgagcctg
      301 tctattccta tccctaatcc accggtagga ggcatcccat attctaatgc ttctataaag
      361 tcttcatcca taggatgtgt ctcctcttct cccatcatct tttctcttaa ttgtgcctca
      421 aatctttccc tctgctctac agggttattc aattcagaaa aagcattcgc cacctctaag
      481 ccgccaataa atagttcaaa tctatctgta aatcttgggt cattctccct tgctttagcc
      541 agtggtgata ccgctacagg ataacctgtg ataaatgttg gtctttttaa ttttggttgt
      601 atttcctcat cgaaaagccc cattaatact ttagcccaga gactttcctc ggtggtcacc
      661 tttatttcga gtttactggc atattctaat aaatcttctc tggttaaatc cagaggattt
      721 actcctgtat attctattac tatatctagt aactctctcc tatcccatgg gggggatata
      781 tctatctcct cattgtctct atatatgatc ggtttaccaa ataatgtgct agctaaatgt
      841 acgattagtt cctccgtcag agacatcatt gtattataat ctgcataagc ctggtaaagt
      901 tccatcattg taaactcagg attatgtacg gtggagattc cttcattcct gaaacacctg
      961 tttatctcaa atactttctc atatccagcc actattaacc tcttaagata caactcaagc
     1021 gctatcctga gatacaggtc aatatttaat gcattatggt gagttatgaa tggacgggct
     1081 gcacctccac cagggactgt atgtaagatt ggagtctcaa cctctataaa tcccatgtct
     1141 tctaggaatt ttcttatctc ttttatcatc ctggctctaa ggagaaatat ctttctggag
     1201 ttttcgtttg ctatcagatc tagatatctt ctcctgtatc tggtttccac atctgttata
     1261 ccgtgccatt tttctggtaa aggtctcgat gctcttgtaa ggatgtagaa tttgtgaatc
     1321 cgtactgaag gttctcctgt gtgggtttta aaagtgaccc cttctacccc tataaaatct
     1381 cccacatgac actcgatcag ttcttcataa gagtcaccca gatcatctgc tttaccatat
     1441 aactggatcc tatctttcag atttcttata tccagaaaca tagatcttcc atgtcttctt
     1501 atagcggtaa ttcttcctgc tattctcacc gtctgctcga ttgttagatc gaagttgctt
     1561 ttcaattcat ctatggaatg tgttggagta aagtccacag aaggttacct ccttatgtcc
     1621 aagactttaa attcgatttc tccccttggt attttgactt ttattatatc acccctggta
     1681 gctccgatta tagcctttcc aacaggagat tcgtcggata ttttattctc cttgatattt
     1741 acctctggag aacttactat atgatattca tttatggagt tatcaattag atttagaagt
     1801 tttaccctgc aacctattg
//
LOCUS       DAOTKU010000356         1816 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_52069_length_1816_cov_3.254401, whole genome shotgun sequence.
ACCESSION   DAOTKU010000356 DAOTKU010000000
VERSION     DAOTKU010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52069_length_1816_cov_3.254401"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            48..>1816
                     /locus_tag="P5310_07620"
     CDS             48..>1816
                     /locus_tag="P5310_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRU33263.1"
                     /translation="MTEKIEIEEKRRWLYEKLKEYRSIDSLNERLDILEEIVKQGYKK
                     DLYLKRLARILVENQRYSEAIKILDDIRNKDIITLSLMVRSLSYLGRYEEAKKCLDKV
                     LGMVELNYDLPYVLSQVSFYYFLRGETREGLALLNSYIEDIINIPSSKRYLANFYNRL
                     AILNYVDGNIENALHFYNLASDYAKRSNDKLILYRIMNNAGDVGRYLYGPKSSIKYNL
                     EAYEMAKDFPKDFIVVSLSNLINAKAQFCSSNEIEAMLKELEEMLKDVNIEYFLYIGY
                     RRLALVNIYYHRSEEIRRFIPYLRNIKSIPENNVLIKIFEGYLGEDIDLLSLEEDVLE
                     TKEIQIILIYLRLLLEKGLTPRKITKEFYSEFPLYQFMKGLIEKEDPISLLKYIDSML
                     ERWEYLDALNSYLLLIKTAGLQDHSSLQHLSYFEAMSVSHLLNLNSIAEELRKEIPLN
                     GFPVDEYARVRIIEYYFKEALINSENEEQAIRLIYRVLSEYLDDFLIRVDVASRTIEK
                     GNTLFGTGNEKLRYENSPFSITLYSREEPNSLTLSLLRSFLNAFIIFWERRYGMYDAL
                     TGLLNRAYGIKRIEESFNEYKRN"
BASE COUNT          632 a          256 c          362 g          566 t
ORIGIN      
        1 aaaatttttc cttcttaata tttaattccc tggggaggaa tgtaactttg acagagaaga
       61 tagaaataga agagaaaaga agatggcttt atgagaagct gaaggaatat agaagcatcg
      121 atagccttaa tgagagatta gatatactag aggagattgt caaacagggg tataagaagg
      181 acctctacct aaagaggctg gcgagaatac tggtagagaa tcagagatat tcggaagcga
      241 taaagatact ggatgatatc agaaacaaag acattataac tctctcttta atggtcaggt
      301 ccctttccta tctgggtagg tatgaggagg caaagaagtg cctagataaa gtcttaggaa
      361 tggttgagtt aaactatgac cttccctatg ttctctccca agtctctttt tactattttt
      421 tacggggaga aacgagagaa ggtttagctt tgctaaattc gtatatagag gatattataa
      481 acattccttc ttctaaaaga tatcttgcta atttctataa tcgtttggct attttaaatt
      541 atgtagatgg aaatatagag aatgcactgc atttttataa tctggcttca gattacgcca
      601 aaaggagcaa tgataagctt atcctctaca ggattatgaa taatgcagga gatgtgggaa
      661 ggtatctcta cggtccaaag agctctatta aatataattt agaagcgtat gaaatggcaa
      721 aagattttcc caaggacttt atagtggtgt ccctgtctaa ccttataaat gccaaggcgc
      781 aattttgcag ctcaaacgag atagaagcta tgttaaaaga attagaagag atgttaaaag
      841 atgtaaatat cgaatatttc ttatatattg gttacaggag attagctcta gtaaatatat
      901 actatcatag atctgaagag atcagaagat ttatccccta tcttaggaac ataaagagta
      961 ttccagaaaa taacgtacta ataaagatat ttgaaggata tctaggagag gatatagatc
     1021 tactatcttt agaagaagat gtcttagaga caaaggaaat ccagataatt ttgatttatt
     1081 tgagattact attagagaag ggattgacac ccagaaagat tactaaagag ttttattctg
     1141 aattccccct gtatcaattt atgaaggggc tgatagaaaa agaagatcca atatcacttt
     1201 taaaatatat agattctatg ttagaaagat gggaatatct ggatgcatta aactcttacc
     1261 tgcttcttat aaaaactgct ggtttgcagg atcattcttc acttcagcat ctttcctatt
     1321 ttgaggctat gagtgtcagt catcttctaa acctaaatag tattgctgaa gagttgagaa
     1381 aagagatacc tcttaatgga tttcctgtag atgaatatgc aagggtaaga attattgagt
     1441 attattttaa ggaagccctc attaattctg agaatgagga acaagctatc agacttatct
     1501 atagggttct ttctgagtat cttgatgatt ttctgataag agttgatgtc gcttcaagaa
     1561 ccatagaaaa agggaatacc ctttttggga ctgggaatga aaaacttcgt tatgaaaact
     1621 ctcctttttc tataactctt tattccagag aagaaccaaa ctcattaaca ctttctcttt
     1681 taaggtcttt tcttaatgcc tttatcatct tttgggagag aagatatggg atgtatgatg
     1741 ctctgacagg acttttaaat agggcttatg gaatcaaaag aatagaagag tcattcaatg
     1801 agtataaaag aaataa
//
LOCUS       DAOTKU010000357         1814 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_52146_length_1814_cov_3.456509, whole genome shotgun sequence.
ACCESSION   DAOTKU010000357 DAOTKU010000000
VERSION     DAOTKU010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1814
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52146_length_1814_cov_3.456509"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            141..1061
                     /locus_tag="P5310_07625"
     CDS             141..1061
                     /locus_tag="P5310_07625"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HRU33264.1"
                     /translation="MISAILTIILLLGVGYVARILGLFKPQDVSLLNRLVLYIFMPAT
                     VFYGIYNLDVNASLLRLPLIAILAILLLLPLLMIFTRGMEGKLRGAFILTALFGNTAF
                     IGYPVSLLTYGKEGLARAIIYDQIGFTIALTTAGVWIARHYGRGESTFSLKETLIGII
                     TYPPFPLLVVALLLRSFKVVLPDFLMSSLSYLSNATVPVIMIGIGGLLQLEFNRKYLK
                     LICSTVLLKLIIFPVVSVLIGILLRLDLLSLKVLIVESGVPVMMSSVVLGSEYGLDVD
                     FILTAVLVTTLLVLLTLPVMNSILLRLLPC"
     gene            1147..1716
                     /gene="infC"
                     /locus_tag="P5310_07630"
     CDS             1147..1716
                     /gene="infC"
                     /locus_tag="P5310_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HRU33265.1"
                     /translation="MNNQIRSAQIRLIDANGRHIGVVPLKEGLRLAKEAGLDLVEINP
                     GANPPVCKILDFGKFQYEKNKKDKEAKKKQKIIDIKEIRLRPEIDLHDLETKIKGAKE
                     FLAEGNKVKITVRLRGRQFLHTDRSSTLVNQIVELLKDVAVVESKKPISEENREFTII
                     FSPKRQEEKKDGKGAKDTQGSIEEVQSNR"
     gene            1658..>1814
                     /gene="rpmI"
                     /locus_tag="P5310_07635"
     CDS             1658..>1814
                     /gene="rpmI"
                     /locus_tag="P5310_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015595206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HRU33266.1"
                     /translation="MGKGLKTHRGASKRFRVTGSGKIMRVHAYTGHHFMKKSKSALRV
                     LKSPAEVD"
BASE COUNT          526 a          311 c          440 g          537 t
ORIGIN      
        1 ctgcgaaagt tgtgggtcca ggaatgtaaa gagacttatt tcctcattca gtttttccag
       61 ctccggagag accggagtaa gcacagctgc ttccagttgc agtacctgta gctctaagaa
      121 ttgcagtatc tgcagataaa gtgatatcag caatactgac tattatttta ctgcttgggg
      181 ttggatatgt agcgcgcatc ctggggttgt ttaagcccca ggatgtttcg cttttaaata
      241 gacttgtcct gtacatattt atgccggcaa ctgtcttcta cggtatatat aatcttgacg
      301 ttaatgcttc tctcttaaga cttcccctga tcgctatctt agctattctt ctcctgttac
      361 cgttattgat gatatttaca agggggatgg agggtaagct tagaggagcc tttatattga
      421 cagctctatt tggcaatacc gccttcatag ggtatccagt atctctatta acttatggga
      481 aggaaggact tgccagagct ataatctatg accagatagg ttttacgata gctcttacca
      541 cagctggagt atggatagcc cgacactatg ggagaggaga gagtactttc tctctgaaag
      601 aaaccctgat aggtatcatt acctatcccc cctttcctct tctggtagtg gctcttcttc
      661 ttcgatcgtt taaagtggtg ctccccgact ttctaatgag ctctctatca tatctgagca
      721 atgccactgt tccggtaatt atgataggta taggtggtct tttacagcta gagttcaaca
      781 ggaaatatct taaattgata tgtagcacag tccttttaaa gttgattata tttccagttg
      841 taagcgtatt gatagggatt cttcttaggt tagacttact ctctctgaag gtattgattg
      901 tagagtcagg tgttcctgtt atgatgtcct cggtggtgct gggaagtgag tatggtctgg
      961 atgtggattt catactgacc gctgtacttg taaccaccct gctggtgctt ctaacacttc
     1021 ctgtaatgaa ttcgatttta cttcgactct taccgtgttg acagcaggtg cctttttgtg
     1081 atatattcta ccagtggaaa ttgaagtatt aaaaggaggg attcgtagat aagtaacgag
     1141 ataaagatta ataaccagat tcgatcagct cagattaggt tgatagatgc caatggaagg
     1201 catataggtg tggtaccttt aaaagagggt ttgaggttag cgaaagaggc aggtttggat
     1261 ttagtggaga taaatccggg agctaatcct ccagtatgca agatactgga ttttggcaaa
     1321 ttccagtatg agaagaataa gaaggataaa gaagccaaga agaagcagaa gataatagat
     1381 ataaaggaga taagacttag acctgagatt gacctccatg atctggagac taaaatcaaa
     1441 ggagctaagg agtttttagc agaaggtaat aaggttaaaa ttactgtaag gcttagagga
     1501 aggcaattct tgcatactga taggagtagt actctggtga atcagattgt agagctatta
     1561 aaagatgttg ctgttgttga atctaagaag ccgatatctg aagagaacag agaattcacc
     1621 ataatatttt cgcccaagag acaggaggaa aagaaagatg ggaaaggggc taaagacaca
     1681 caggggagca tcgaagaggt tcagagtaac aggtagcggg aagataatga gagtccatgc
     1741 ttacactgga catcacttta tgaagaagag taagtctgca ctgagagtgc ttaagagccc
     1801 tgctgaggtg gata
//
LOCUS       DAOTKU010000358         1813 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_52186_length_1813_cov_3.481797, whole genome shotgun sequence.
ACCESSION   DAOTKU010000358 DAOTKU010000000
VERSION     DAOTKU010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52186_length_1813_cov_3.481797"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..980
                     /locus_tag="P5310_07640"
     CDS             <1..980
                     /locus_tag="P5310_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013433557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HRU33267.1"
                     /translation="ISLNASESLARAAVEVGTYWNTGEGGLHRKLYPYSHRAIVQCAS
                     GRFGVDIDYLNAGSAIEIKIGQGAKPGIGGHLPGEKVGEDVSSTRMIPIGTDAISPAP
                     HHDIYSIEDLKQLIYALKEAVNYKKPVGVKIAAVHNVSAIASGIVRAGADFIAIDGFR
                     GGTGAAPTRIRDNVGIPIELALAAVDTRLREEGLRHQVSIIAAGSIRNSADVIKAVAL
                     GADAVYIATSALVSLGCHLCQNCHTGRCNWGIATQDPNLVKRLNPDIGARRASNLLNA
                     WAHEINEMLGGMGINALESLRGNRLMLRGIGLNEKELEILGIKHAGEGM"
     gene            981..1418
                     /locus_tag="P5310_07645"
     CDS             981..1418
                     /locus_tag="P5310_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRU33268.1"
                     /translation="MESKIRKRIYPKEDVCIGCHLCEVYCILAHSGSKDLVKAFNKKD
                     RPSPRVTVEEKDGYYITFALQCRHCDDPLCIKACITGAMYRDEKTGVIMCDESKCVGC
                     WTCILVCPYGAIRRGENKKVASKCDLCIERGTPICVENCPNEA"
     assembly_gap    1425..1434
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1458..>1813
                     /locus_tag="P5310_07650"
     CDS             1458..>1813
                     /locus_tag="P5310_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRU33269.1"
                     /translation="MRYVIIGNSIAATGCIEGIRRVDTEGEIIVISEEPYSVYSRPLI
                     SYWLSGKIGDNDIYYRPGDFYRKNQVKLISGVKATRVDFDGKEVFLEAGEGIHYDKLL
                     IATGGKPFVPPIPGPKG"
BASE COUNT          569 a          291 c          486 g          457 t
ORIGIN      
        1 cgataagtct caatgcctct gaatcactgg caagagcagc ggtagaggtt ggaacatact
       61 ggaatacggg agagggaggc ttacatagaa aactatatcc atacagccat cgtgctattg
      121 ttcagtgtgc gtctggaaga tttggtgttg atatagatta tttaaatgcc ggttctgcta
      181 tagagataaa gattgggcag ggtgcaaaac caggtatcgg aggacatcta cctggagaaa
      241 aagttggaga ggatgtatcc agtacaagaa tgatcccaat agggacagat gctatctctc
      301 cagccccaca tcatgatatc tattccatag aagatttaaa gcagttgata tacgcattga
      361 aagaggctgt aaattataaa aagccagtgg gggtaaagat agcagccgta cacaatgtat
      421 ctgccatagc gtctggtata gtaagagccg gtgcagattt tatagctata gatggttttc
      481 ggggaggaac tggagctgcc ccgacaagaa taagagataa tgttgggata cccatagaac
      541 tggctctggc tgctgttgat acaagactca gggaggaagg tttgagacat caggtatcca
      601 ttatagcggc aggttctatc agaaatagtg ctgatgtaat aaaagctgta gcgcttggtg
      661 cagatgctgt ttatatagcg acatctgccc tggtatctct tggttgtcat ctctgtcaga
      721 attgtcacac cggaaggtgt aactggggta tagcaaccca ggatccaaat ctggtaaaaa
      781 ggttaaaccc cgatatcgga gcgagaaggg cgagcaatct attgaacgca tgggctcatg
      841 agataaatga gatgcttgga ggaatgggga taaatgcact tgagagtttg agggggaata
      901 gacttatgct aagagggata ggtctgaacg aaaaagagct ggagatcctt gggataaaac
      961 atgccgggga ggggatgtaa atggaatcaa agataagaaa gaggatatat ccgaaagaag
     1021 acgtatgtat aggatgtcat ctttgcgagg tctactgtat cctggctcat tctggaagta
     1081 aagacctggt taaggctttt aataagaaag atagaccatc ccccagagtt accgttgaag
     1141 aaaaggatgg atactatata acatttgcat tgcagtgtcg tcactgtgat gaccctctat
     1201 gcatcaaagc ctgtattaca ggtgccatgt atagagatga aaagacaggg gttattatgt
     1261 gtgatgaaag taagtgtgtg gggtgctgga cctgtattct ggtatgtcct tacggtgcta
     1321 taagaagagg agaaaataaa aaggttgcct ctaaatgtga cttatgcata gagagaggca
     1381 ctccgatctg cgtagaaaat tgccccaacg aagcatagag ctgannnnnn nnnngaaaga
     1441 ggtatgagga ggagactatg aggtacgtca tcattggtaa ttccatagca gctacagggt
     1501 gtatagaggg gataagaagg gttgataccg aaggagaaat tatagtaatc tctgaagagc
     1561 catattctgt atattccaga ccgttaatct catactggct ttcggggaag attggagata
     1621 atgatatata ttatagacct ggagattttt acaggaagaa tcaagttaaa ctgatctctg
     1681 gagtaaaagc caccagggta gattttgatg gtaaggaggt atttttagag gcaggagaag
     1741 gtatacacta tgacaaatta ctgattgcta ccggtggtaa accctttgtc cctcctatac
     1801 caggtccaaa agg
//
LOCUS       DAOTKU010000359         1807 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_52414_length_1807_cov_3.209475, whole genome shotgun sequence.
ACCESSION   DAOTKU010000359 DAOTKU010000000
VERSION     DAOTKU010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52414_length_1807_cov_3.209475"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            29..583
                     /locus_tag="P5310_07655"
     CDS             29..583
                     /locus_tag="P5310_07655"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRU33270.1"
                     /translation="MPAKIVHDISNLGSASIKDILIPFFTAIFLHGGWYHILSNMLYL
                     WIFGDNLEDRMGHLRYLLFYLLCGFTSSLAHIITNISSPIPTIGASGAISGVLGAYFI
                     IFPEARVLTLVPFGFFLHIAAIPAFLFLGFWFFSQTLNGLASLAIRTSMTGGVAWWAH
                     IGGFVTGAIIGIRYRRRYRPYYGW"
     gene            596..1117
                     /locus_tag="P5310_07660"
     CDS             596..1117
                     /locus_tag="P5310_07660"
                     /inference="COORDINATES: protein motif:HMM:TIGR01947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HRU33271.1"
                     /translation="MGEHIKTSLLLAIVCIVSAAALGYFYKLTTPIIEQRQISDQISL
                     QREVLPQADDFKETKIDGENVYLGISSGKIVGGVAKVDVKGYGGTIELIVGIDSTGKI
                     TGVRVLSHSETPGLGEKATKDNYLSQFIGKGSSEINTVRAITGATISSNAIRNGVNEG
                     MQIVLKAMGSQKL"
     gene            1114..>1807
                     /locus_tag="P5310_07665"
     CDS             1114..>1807
                     /locus_tag="P5310_07665"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HRU33272.1"
                     /translation="MKRRLFSATEEEIINGETADVYFERTYKVLESIGELDTPVIGEI
                     FARRGAYLGGVREALNLFEGRKIRVYSLEEGEPFKEKEVIMRIEGPYGEFGIYETAIL
                     GILASSTGWVTAARECKEASNGKPVLSFGARHLHPAIASVMDRAAIIGGADGVSSILG
                     GELVGIIPSGTMPHSLILIIGDTVEAARAFHMVHPEISLIVLVDTFRDEAEEAIRVAR
                     EFGRSLYGIRLDT"
BASE COUNT          577 a          287 c          434 g          509 t
ORIGIN      
        1 ggaaagattt atatttcaat atggaatagt gccagccaag atagtacatg atatctccaa
       61 tttaggctct gcctccataa aggatatttt aattcctttt tttacagcga tattcttgca
      121 tggtggctgg tatcatatac tgagtaatat gttatatcta tggatattcg gagataattt
      181 agaagatagg atgggacatc taagatatct tcttttctat ctactatgtg gttttacctc
      241 cagtctggcg catataataa caaatatatc ttcccctatt cccaccatag gtgccagtgg
      301 agctatatct ggagtactgg gtgcttattt tataattttc cctgaggcta gagttctgac
      361 attagtacca tttggatttt tcctccatat agcagctata cctgcctttc tatttttggg
      421 attctggttt ttctctcaaa ccttaaacgg attagccagt cttgccataa ggacatctat
      481 gacaggagga gttgcctggt gggctcatat aggaggattc gtcacaggag cgataattgg
      541 aatcagatat agaagacgtt acagaccgta ttacggttgg taaggaggaa atagtatggg
      601 tgaacatata aagacatctc ttttattagc tattgtctgt attgtctctg cagctgcact
      661 aggatatttc tataaactta caacaccgat aatagaacag aggcagattt cagatcagat
      721 ctctctgcag agagaagtct tgcctcaggc tgatgatttt aaagaaacaa aaatagatgg
      781 agaaaatgta tatctaggaa taagtagcgg aaagatcgtg gggggagtag ctaaagttga
      841 cgtaaaagga tatggtggga caatagaatt gattgtggga atagactcta caggaaagat
      901 tacaggggtg agagtcctct ctcatagtga gactcctgga ttaggagaaa aggctaccaa
      961 agataattat ctctctcagt tcataggtaa gggcagttct gaaataaaca ctgtaagggc
     1021 tataactggg gcaacaatat ctagtaatgc aatcagaaat ggggtaaatg aaggaatgca
     1081 aatcgtttta aaagccatgg ggagtcaaaa attatgaaaa gaagactatt ctcagctact
     1141 gaagaagaga taattaatgg ggaaacagct gatgtatatt ttgaaagaac atataaagtc
     1201 ctagaatcta taggggaact tgatacccct gtaatcggag agatattcgc caggagaggg
     1261 gcataccttg gaggagtaag agaagcattg aatctttttg agggaaggaa gatccgggta
     1321 tactctctgg aagaaggaga accattcaaa gagaaagaag taattatgag gatagaaggt
     1381 ccatatggag agtttggaat atatgaaacc gcgatacttg gaattctggc atcttctaca
     1441 ggctgggtaa cagcggcaag agaatgtaag gaggccagta atggaaaacc agtcctctca
     1501 tttggagcca gacatctgca tcctgcaata gcttcagtaa tggatagggc agcaatcatt
     1561 ggtggagctg atggtgtaag ttctatactt ggaggagaac tagttggaat aattccatct
     1621 ggaacaatgc cacattcttt aatacttatt attggagaca cagtggaagc agcaagggca
     1681 ttccatatgg tacatccaga gatatctcta attgtacttg tagatacatt cagggatgag
     1741 gcggaagagg ctataagggt agccagagag tttggcaggt ctctatatgg tatcaggctt
     1801 gatacac
//
LOCUS       DAOTKU010000360         1799 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_52755_length_1799_cov_2.953555, whole genome shotgun sequence.
ACCESSION   DAOTKU010000360 DAOTKU010000000
VERSION     DAOTKU010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52755_length_1799_cov_2.953555"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..772)
                     /gene="secA"
                     /locus_tag="P5310_07670"
     CDS             complement(<1..772)
                     /gene="secA"
                     /locus_tag="P5310_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376163.1"
                     /note="functions in protein export; can interact with
                     acidic membrane phospholipids and the SecYEG protein
                     complex; binds to preproteins; binds to ATP and undergoes
                     a conformational change to promote membrane insertion of
                     SecA/bound preprotein; ATP hydrolysis appears to drive
                     release of the preprotein from SecA and deinsertion of
                     SecA from the membrane; additional proteins SecD/F/YajC
                     aid SecA recycling; exists in an equilibrium between
                     monomers and dimers; may possibly form higher order
                     oligomers; proteins in this cluster correspond SecA1;
                     SecA2 is not essential and seems to play a role in
                     secretion of a subset of proteins; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HRU33273.1"
                     /translation="MFKLLEKFIGKDSDRELRRYWRIVEKINELEDEISRLSDEELAG
                     KTLEFKDKLNNGATLDDVMVPAFAVVREVARRKVNMRPFDVQLIGAIVLHEGKIAEMK
                     TGEGKTLVATMPAYLNALTGEGVHIVTVNDYLAKRDRYWMGPVYEALGLKVGLLQHDS
                     SPAERRDAYNSDITYGTNTEFGFDYLRDNMALSRDELVQRGFNYAIVDEVDSILIDEA
                     RTPLIISGVADKPTRLYYQFAQIARNLVRDMDYTVDEKL"
     gene            complement(785..>1799)
                     /locus_tag="P5310_07675"
     CDS             complement(785..>1799)
                     /locus_tag="P5310_07675"
                     /inference="COORDINATES: protein motif:HMM:NF016364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HRU33274.1"
                     /translation="EVRYVGLEFGIGGLMPHQASEVFKYKYGDCKDKATLLISMLREI
                     DVPAHYVLVRTRYIGSLPLESPGFQFDHAICAIKLDGKLYFLDGTAEDTRFGYIPSMD
                     QGTKALLIEDGKPVLVDIPLDKPEDNEKTRLIDVSLSKDGSIEGTSYITMKGYYDTYL
                     RNVWKSASDREIRENLEWSLRAFIPNAILGGYEISNLRDLNSDVWEKYSFKAPGYADV
                     VGNTLSFLPSVMERLETSESVAKEERVYPINYWSPYTTRDIINYKLPDNCKIVHLPED
                     LVFDKSFGYYSVKFTVEGNILKVERVFQIRSILIPPDIYKEYKAFIEGIVSKDRERVV
                     ISF"
BASE COUNT          499 a          445 c          287 g          568 t
ORIGIN      
        1 taagtttctc atctacggta taatccatat ctcttaccag attccttgct atctgggcaa
       61 actgatagta taatcttgtc ggcttatcag ctaccccgga gataataaga ggagtcctgg
      121 cttcgtctat caggatactg tcgacctcgt caactatagc ataattgaag cctctctgta
      181 caagttcatc cctgcttaaa gccatattat ctctaagata atcaaaacca aactcagtat
      241 ttgttccata ggtaatgtca gaattatagg catctcttct ctcagctggg ctggaatcgt
      301 gctgcaagag tccaacctta agccccaggg cttcatatac aggtcccatc cagtatctat
      361 ctctttttgc cagatagtca ttcactgtga ctatgtgcac cccttctccg gtcagtgcat
      421 ttagataggc aggcattgtt gcaactaaag tcttcccttc ccccgtcttc atctctgcta
      481 ttttgccctc atggagtacg atagccccta tgagctggac atcaaaaggt ctcatattga
      541 cctttctccg ggcaacctcc ctaacaacgg caaaggcagg gaccattaca tcatccagtg
      601 tagctccatt atttaattta tccttaaact ctaacgtttt acctgcaagt tcttcatcgg
      661 aaagacgaga tatctcatct tccaattcgt ttatcttttc tacaatacgc cagtatcttc
      721 tcagttctct atctgaatct tttccaataa acttttctaa cagtttaaac atagtaactc
      781 tcctctagaa gcttattacc accctttctc tatccttaga tactatccct tctataaaag
      841 ccttatactc tttatagatg tcagggggga taagtatgct tctaatttga aagactctct
      901 ccaccttcag gatattccct tcaactgtaa acttcacact gtaatatccg aatgacttat
      961 caaagaccag atcttcggga agatgaacga tcttacagtt atccgggagt ttatagttaa
     1021 taatatctct ggtggtgtaa ggagaccagt aatttatagg ataaactctc tcctctttag
     1081 ccacagactc gcttgtttca agcctctcca taacagatgg caagaaggac agtgtatttc
     1141 ccaccacatc tgcatatcca ggagctttaa agctatactt ctcccataca tcagaattta
     1201 aatctctcag attagagatt tcatatccac caagaattgc attggggata aatgcacgta
     1261 aagaccattc caaattttct cttatctccc tgtcacttgc agatttccat acatttctca
     1321 gataggtatc ataataaccc ttcatcgtaa tataagaagt gccttcaata cttccatcct
     1381 ttgataaaga gacatctata agtcttgtct tctcattatc ctccggctta tctaaaggta
     1441 tatccaccag aactggtttt ccatcctcta tcaatagtgc ctttgtcccc tgatccattg
     1501 agggtatata tccaaaacgg gtatcttcag ccgtcccatc cagaaaatat agctttccat
     1561 caagttttat ggcacatatc gcatggtcaa actgaaaacc tggagattct aaaggtagcg
     1621 agcctatata tctggttctg accagtacat aatgggcagg gacatctatc tctctgagca
     1681 ttgatatgag gagtgtggct ttatctttac aatctccata cttatacttg aagacctcac
     1741 ttgcctggtg tggcataaga ccacctatac cgaattcaag tcccacatat ctgacctca
//
LOCUS       DAOTKU010000361         1794 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_52988_length_1794_cov_2.563542, whole genome shotgun sequence.
ACCESSION   DAOTKU010000361 DAOTKU010000000
VERSION     DAOTKU010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52988_length_1794_cov_2.563542"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            104..1150
                     /locus_tag="P5310_07680"
                     /pseudo
     CDS             104..1150
                     /locus_tag="P5310_07680"
                     /inference="COORDINATES: protein motif:HMM:NF018540.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
     gene            1151..>1794
                     /locus_tag="P5310_07685"
     CDS             1151..>1794
                     /locus_tag="P5310_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU33275.1"
                     /translation="MRKSLSITVVILILFELVPASVYASQNKLRVGFLGVFMENKEEP
                     SLSSMVSPMFSDSKFFVWEKITKDTPTSIFWGVARIDAKFQHNSNSVSGRFTLYIREP
                     STNVDLFVLDATGVSSTFSGMVVDWSPFEKEALLNGLSVLSRRLEDLWRSNSIVIYSE
                     GKVFECDMGQLAGIVEGSHLSVFRDNRLIAKGKITGLDEKKCRAEIIYSSGDKTP"
BASE COUNT          515 a          298 c          442 g          539 t
ORIGIN      
        1 cagggagata accaggaaaa acgagcttaa agtcctttct gcctttctag atgctggaat
       61 agatgagtat accgtttctt ttgtgtgaac cggttatgga tatggagata ggggaaggga
      121 tgccctttcc agggtctttt ccctttctct atccgcagaa tctgccatag tactgcccca
      181 gataatctca gggactcacg caatctccag tgcactattc gctcttctga gaccgaaaga
      241 caggatactg tcccttaccg gtccagttta cgatacgctg gagaaggtca tagattcgtt
      301 gaaagagtgg gagataggat atcaggagat cccatttgaa cgtagattgg ataccgatta
      361 tattgatagc tcagtagaaa ttcccccaaa actcatattt atacagcgtt cctgcggtta
      421 taactggtcc aggaagtctc taactataga tgagataaat gaattgattc ttaatataaa
      481 gcgcatattt gatgaaagta ttatactcgt tgacaattgt tacggagaat ttgtagaaga
      541 tagagaacct ctagatgcag gtgccgatat tattgtcggg tctttaatta aaggggtggg
      601 aggaaatctc gcacctacag gtggatatat agccgggaag aaagatgtaa tagacaggat
      661 aggatcaacc gttaccgctc caggacaggg aagggatatt ggtcctacat tgggacttca
      721 gaaggatata cttcagggat tgctctttgc tcctcacttt ataggggaat ctttgacagg
      781 tctgacaata tcttcctata ttttagaaca tatgggtttt aagactctac cagagtggaa
      841 tgaggatagg ggagatgctg tattgagagt gattctgaat aatgaaaaaa gccttataaa
      901 cttcttacag gggatacaga gttcctgccc tgtaaattct atgtataagc cagagcctca
      961 ggaaattcca ggttatcctg ccccagtgat cctggcagga ggaggttttg ttcagggctc
     1021 taccggggag tttagtgcgg atggtatatt aaagcctccc tatgttgctt ttattcaggg
     1081 gggttattct tctaatctgg taacttttgg tataattaaa agtataaaga agattttaga
     1141 ggagggttaa atgaggaaga gtttaagtat taccgtggta attttaattc tttttgaact
     1201 tgtcccagca agtgtatatg cttctcagaa taaactgagg gtgggttttt tgggtgtatt
     1261 catggagaat aaagaagagc cgtctctcag tagtatggtc agccctatgt tctctgatag
     1321 taaattcttt gtctgggaga agataactaa agatactcca acatcaatct tctggggtgt
     1381 ggcaaggatt gatgcaaagt ttcagcataa ttctaatagt gtctctggca ggtttacctt
     1441 atatataaga gagccttcca ctaatgttga tctgtttgtc cttgatgcta caggagtgag
     1501 ctctactttt tctggaatgg tggtggattg gagtcctttt gaaaaggaag ctcttcttaa
     1561 tggtctatca gtcctttcac ggaggctcga agatctatgg aggagtaaca gcatagttat
     1621 ttactctgaa gggaaagtct tcgaatgtga tatgggacag ttagctggca tagttgaggg
     1681 aagtcatctg agtgtattta gggataacag gcttatagct aaaggtaaga ttacaggttt
     1741 agatgaaaaa aaatgcaggg cagagattat atacagctct ggggataaaa ctcc
//
LOCUS       DAOTKU010000362         1793 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_53055_length_1793_cov_2.390679, whole genome shotgun sequence.
ACCESSION   DAOTKU010000362 DAOTKU010000000
VERSION     DAOTKU010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53055_length_1793_cov_2.390679"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="P5310_07690"
     CDS             <1..429
                     /locus_tag="P5310_07690"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HRU33276.1"
                     /translation="QNKVPAFFVDKIEDLPELPTTKVLGIGDPDIVRKVQVQLKERYN
                     SNLYITTSSPTYLEIMNRDVSKGRALSILSSITGISVSEMAGLGDSFNDLELIMDSGL
                     GITFDNAPLELKENADYVTGLSSPEGFREVVDFILWKNLY"
     gene            411..1280
                     /gene="rapZ"
                     /locus_tag="P5310_07695"
     CDS             411..1280
                     /gene="rapZ"
                     /locus_tag="P5310_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HRU33277.1"
                     /translation="MEESLLNLIIITGFSGAGKSLAIRYYEDMGYFCIDNLPVTLIPK
                     LVELCLQSDGKIKNVALVIDIRGKEFFDSLKNSLETVHKMGVKEKIIFLEATEEVLIR
                     RYEETRRPHPLGEFGTLSENIRKEKRALEPIREMADYVIDTSRIPPSQLKEVLQKLSL
                     FKAKDTIVFNLVSFGYKFGIPLDADLILDVRFLNNPFYEPELKYKSGLDKEVKDYVMG
                     DAAAEGFLERVADLIEFLVPQYIRLGKDQITIGIGCTGGRHRSPAIVDVLRKKLEDEG
                     YRVTVTHRDIEAE"
     gene            1284..>1793
                     /locus_tag="P5310_07700"
     CDS             1284..>1793
                     /locus_tag="P5310_07700"
                     /inference="COORDINATES: protein motif:HMM:NF025880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU33278.1"
                     /translation="MERYSESLRFELCKKVPQDIQEAIVELQGIVGSRKNIKKNYIYT
                     DRMNVANRVTLLWKTVFNSTVNLDIKKHKDTYRFYIKLDESYYDLLEWKGELPDYFLR
                     GVFIACGFISDPEKGYRIELIPINREFTRYISKALDSLSLRYNVYGKRVYINGFSNAE
                     RFLSLIGVQH"
BASE COUNT          592 a          262 c          416 g          523 t
ORIGIN      
        1 cagaataaag tcccggcatt tttcgtagat aagatagagg atttaccaga gcttccaacg
       61 acaaaagttc tggggattgg agatcctgat atagtaagaa aagttcaggt ccagttaaag
      121 gaaaggtata atagtaatct ttatataact acaagtagtc cgacctatct ggagattatg
      181 aatagagatg tctccaaggg aagagctcta agtatcctgt ccagtatcac aggaatctct
      241 gtctctgaga tggctggttt aggagatagc tttaatgatc tggagcttat aatggattct
      301 ggtcttggaa taacatttga taatgctccc ctggagttga aagaaaatgc agattatgta
      361 acgggacttt catctccaga gggttttaga gaggtggtgg attttatact atggaagaat
      421 ctttattaaa tcttataata ataactggat tttctggtgc aggtaaatct ctcgctataa
      481 gatactatga ggatatggga tatttttgta tcgataatct ccctgtcact cttataccca
      541 aacttgtaga actatgcctt cagtctgatg gtaagataaa aaatgttgct ttagttatag
      601 atataagggg taaagagttt tttgattctc taaagaattc cctggaaaca gtacataaaa
      661 tgggggtaaa agaaaaaata atcttcctgg aagctactga ggaagttctg ataaggaggt
      721 atgaagaaac ccgtagaccc catccccttg gagaatttgg aacgctttct gagaatataa
      781 ggaaagaaaa aagagcttta gagccgatca gagaaatggc ggattatgtg atagatacat
      841 ccagaatacc tccgtcacag ttgaaagagg tccttcagaa gctctcattg tttaaggcta
      901 aggatactat agtatttaat cttgtgtcat ttggttataa gtttggaatc cctctggatg
      961 cagatttaat cctagatgtg agattcctga ataatccatt ttatgagccc gaattaaaat
     1021 ataagagcgg attggataaa gaggtaaaag attatgtgat gggggatgct gctgcagagg
     1081 gttttttgga gagagtggca gacctgatag aatttcttgt acctcagtac atcaggttgg
     1141 gaaaagatca gataacgata gggataggat gcaccggagg aagacacagg tcaccggcta
     1201 tagtcgatgt cttaaggaag aaactggagg atgagggtta tagggtaaca gttacccata
     1261 gagatataga agcagagtga agtatggagc gatattctga atctttaaga tttgagctgt
     1321 gtaaaaaagt tccgcaagat attcaggaag cgatcgtcga acttcagggt atagttggct
     1381 ccagaaaaaa tatcaaaaag aattatatat ataccgatag aatgaatgtc gcaaacaggg
     1441 taactcttct ctggaagacc gtctttaatt caactgttaa cctggatata aagaaacata
     1501 aagataccta tcgattttat ataaaacttg atgagagtta ttatgatctt ttagagtgga
     1561 aaggagaact ccccgattat tttttgaggg gagtgtttat agcctgcgga tttatctctg
     1621 atccggagaa gggttacagg atagaattaa taccaatcaa cagagaattt accagatata
     1681 tctcaaaagc gctcgattct cttagtctca ggtataacgt atatggaaag agggtttaca
     1741 taaatggatt ttctaatgcc gagagatttt taagtctgat aggtgttcag cat
//
LOCUS       DAOTKU010000363         1792 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_53094_length_1792_cov_2.448474, whole genome shotgun sequence.
ACCESSION   DAOTKU010000363 DAOTKU010000000
VERSION     DAOTKU010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53094_length_1792_cov_2.448474"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..936
                     /locus_tag="P5310_07705"
     CDS             <1..936
                     /locus_tag="P5310_07705"
                     /inference="COORDINATES: protein motif:HMM:NF014517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HRU33279.1"
                     /translation="GLPLVFSEEALESISNYFSIRNRPFGPGTPKEAFIPEGGKILRN
                     NVGVAPGFWIKDKGKDIIVLPGVPREAQDIWENIVRKSLESVSDKIPSYNLRIFGAGE
                     SVVESKILDIIKGNSSDVEILIGEPLEIRLHIRGENGEEIFKKIKERLGDMVFSVDNE
                     TLAEVVVKKLIEKRLTLATAESCTGGLLAERITRIPGSSQTFRGGFIPYTKWAKKTLG
                     IPTYLLDYGDVSLEIAESLARMAKEKFSSDFGIGITGNAGPSQGDTSKPVGYVCISVV
                     NSSSAISRDFQLEGDRERVRFLAVQWSLDILRRSI"
     gene            933..1460
                     /gene="thpR"
                     /locus_tag="P5310_07710"
     CDS             933..1460
                     /gene="thpR"
                     /locus_tag="P5310_07710"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HRU33280.1"
                     /translation="MRLFIAVEIPEDVKEELGKTMEALKRKVSSVKWVEKENFHITLR
                     FIGEVQEDKVQTIEDIIDEVSSNFYPFDIGLKGVGNFPYVIWVGIDKGKDILGDISYS
                     IEGLLIKAGFPPTDKLFSPHITLGRVKKRIENFQGGDFGPLSFQSDSITLMQSQLFSS
                     GPVYTPLKVFPFKKI"
     gene            complement(1515..>1792)
                     /locus_tag="P5310_07715"
     CDS             complement(1515..>1792)
                     /locus_tag="P5310_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011944098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRU33281.1"
                     /translation="TEELFKNPKHPYTEALLAAVPKPDPKYRGRRNLLFGEVADPSNP
                     PSGCYFHPRCPYVQDICKEEHPPLRSLGNDHFASCHFADKLSLVGIE"
BASE COUNT          546 a          299 c          436 g          511 t
ORIGIN      
        1 ggtttaccgc tggtattttc agaggaagcc ctggagagca tcagcaatta tttctctata
       61 aggaatagac ctttcggacc aggaacgcct aaagaggctt ttatccctga gggcgggaag
      121 atattgagga acaatgttgg ggttgctcct ggtttttgga ttaaagataa aggaaaagat
      181 ataatagtct tacctggtgt gccaagagaa gctcaagata tatgggagaa tatagtcaga
      241 aaaagcctgg agagtgtaag cgataaaata ccgtcatata accttagaat ctttggagct
      301 ggtgagtctg tagttgagtc caagatcctt gatatcataa aagggaattc ttcggatgta
      361 gagatactta taggagaacc tctggagata aggctgcata taagaggtga aaacggagaa
      421 gagatcttta agaagataaa ggagagattg ggggatatgg ttttcagtgt ggataatgag
      481 accctggctg aggtcgtagt taagaagctt atagaaaaga gattgacact ggctacagca
      541 gaatcttgca ccggtggatt actggcagag agaataacca ggattcctgg aagttctcag
      601 acatttagag gaggatttat accttatact aaatgggcaa aaaagacact gggtatcccc
      661 acctatctac ttgattatgg agatgtcagt ttagagatag cagagagtct ggcgaggatg
      721 gctaaagaga agttttcttc agattttggg ataggtatta caggaaatgc aggaccttct
      781 cagggtgata catcaaaacc ggtaggttat gtatgtatca gtgtagtaaa tagcagttct
      841 gctatttcca gagactttca gcttgaaggg gatcgggaaa gggttagatt tttagcagtt
      901 cagtggtctc tggatatact gaggaggtct atatgagact ttttatagct gttgagatac
      961 ccgaagacgt taaagaagag ctggggaaaa cgatggaagc ccttaagcgt aaggtctcaa
     1021 gtgtaaaatg ggttgaaaag gaaaattttc atataacgct tagatttata ggtgaagtcc
     1081 aggaggataa ggtccagaca atagaggata taatcgatga ggtaagttct aacttctatc
     1141 catttgatat tggattgaaa ggtgtgggga atttccccta tgtgatatgg gttggtatag
     1201 ataaaggtaa agatatatta ggggatatct cttatagcat agaaggactt cttattaagg
     1261 caggctttcc tcccacagat aaactgttct ctccgcatat cactttagga agggttaaga
     1321 aaagaataga aaactttcag ggaggagatt ttggtccatt atcatttcaa tcggactcta
     1381 taaccctgat gcaaagccag cttttttctt caggaccggt atatactcct ctcaaggttt
     1441 ttccttttaa aaaaatatag gggcaagata taatccgccc ctatagatac atcctttcta
     1501 agataaaata tcttttactc tatccctacc agactcagct tatctgcaaa atgacaggag
     1561 gcaaaatggt cattccccag gctccttaat ggtggatgct cttctttaca tatgtcctgt
     1621 acatatggac accttggatg gaaataacat ccacttggtg gattcgacgg gtccgctacc
     1681 tctccaaata ggagattcct cctacctcta tactttggat ctggcttcgg aactgctgct
     1741 aacagtgctt ctgtgtatgg atgcttcgga ttcttaaata gctcctccgt ct
//
LOCUS       DAOTKU010000364         1785 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_53376_length_1785_cov_2.592486, whole genome shotgun sequence.
ACCESSION   DAOTKU010000364 DAOTKU010000000
VERSION     DAOTKU010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53376_length_1785_cov_2.592486"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="P5310_07720"
     CDS             complement(<1..281)
                     /locus_tag="P5310_07720"
                     /inference="COORDINATES: protein motif:HMM:NF013328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HRU33282.1"
                     /translation="MPELPELEILRINLNNRIKGKRIGRPTIINPKAISGDPNLLENK
                     KIEEVMRRGKWILFSLDKLYLSFHLRLNGYIFLLDYGEPDDYDCAILPV"
     gene            complement(274..507)
                     /locus_tag="P5310_07725"
     CDS             complement(274..507)
                     /locus_tag="P5310_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010997347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HRU33283.1"
                     /translation="MRITVKVKPNSSKQEIRLKDNIYNVNLLSPPVEGKANRELIELL
                     SDYFNIPKSEVSIISGHKSRTKIVNIEREDLNA"
     gene            complement(504..1370)
                     /locus_tag="P5310_07730"
     CDS             complement(504..1370)
                     /locus_tag="P5310_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HRU33284.1"
                     /translation="MRLIRYPGNPVLKPAYIWEEGGVFNAGAVYHNGLFHLLYRAVYG
                     ENRSNIGYAVSRDGFHFFRLDKPVFEHTGDHETFGAEDPRITKIGDTFYMLYTAYSEI
                     GVRVSLASTKNFIAWTRYGVVLPDVDDKDAVLFPEKINNKYVMLHRIHPDIWIAYSDD
                     LIHWNGHRVIMTPRKGYWDSEKIGAGAPPIKTEKGWLIFYHGVDGDNVYRLGVALLDL
                     EDPSIVLKRQEEPVLEPEKYYELEGIVPNVVFTCGAVEVNDTYYVYYGGGDYVIGVAT
                     VKKEEVRDFIEE"
     gene            1429..>1785
                     /locus_tag="P5310_07735"
     CDS             1429..>1785
                     /locus_tag="P5310_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU33285.1"
                     /translation="MRKDILLFYIAIISISSSQLYLEVSLTRIFSITQGYHWAFLVVG
                     LALLGSGLSGTCLVFRKGLYKNKGIEHLRVLSIIYPLSIILAYLEITLLQFDPYVMPW
                     SRVQFLFFFLNLTSFLV"
BASE COUNT          456 a          423 c          288 g          618 t
ORIGIN      
        1 tctacaggga gaattgcaca gtcataatca tctggttcac catagtctaa taaaaatata
       61 tatccattga gcctcaggtg aaatgataaa tagagtttat ccagagagaa cagtatccac
      121 tttccccttc tcataacctc ttcaatcttt ttgttctcca gtagatttgg atccccactg
      181 attgcctttg gattaattat tgttggtcta ccgattcgct tcccctttat cctgttattt
      241 agattgattc tgagaatttc cagttcgggt agttcaggca tttaaatcct ccctttctat
      301 atttactatc tttgttctac tcttatgacc acttataatc gatacctctg actttgggat
      361 attaaagtaa tctgagagca gctctatcag ttctctgttt gctttaccct ctacaggagg
      421 agagagcaaa ttaacattat aaatattgtc ttttaatctg atctcctgct tggaagagtt
      481 tggtttgacc ttcacagtta tcctcattcc tctataaaat cacgaacctc ctcttttttt
      541 accgttgcca ctccaatcac atagtctcca cccccataat atacataata ggtgtcgttc
      601 acctctaccg ctccacaggt aaacacaaca ttcgggacta tcccttctaa ctcatagtac
      661 ttttctggtt caagtactgg ctcttcctgt ctctttaata cgatagacgg gtcttccagg
      721 tccaagaggg ctactccaag tctgtacaca ttatctccat ctactccatg ataaaatatc
      781 agccagcctt tctctgtttt gattggggga gctccagcgc cgatcttctc actatcccag
      841 tatccctttc tgggagtcat tataacccta tgtccattcc agtgtatcaa gtcatcagaa
      901 taggctatcc atatatctgg atgtattctg tggagcatta catatttatt gttaattttt
      961 tctggaaaaa gcaccgcatc cttatcatcc acatctggta ataccacccc gtatcttgtc
     1021 caggctataa aattctttgt acttgccaga gataccctga cccctatctc tgaatatgct
     1081 gtatagagca tatagaatgt atcgcctatc tttgtaattc ttgggtcctc agccccaaat
     1141 gtttcatggt ctcctgtatg ttcaaagaca ggtttgtcca gtctaaagaa atgaaatcca
     1201 tccctactta ccgcatatcc tatatttgag cggttttctc cataaacagc cctatataag
     1261 aggtgaaaga gtccattatg gtacacggct ccggcattaa ataccccacc ttcttcccag
     1321 atatatgcag gtttaagtac cggattcccc ggatatctga tcagtctcat agatttcctc
     1381 ctccattcta tcttctctta tgtcattatt atatcgtata atatccatat gaggaaagat
     1441 atccttctct tttacatagc gattatctct atatcttctt ctcaactcta ccttgaggta
     1501 tcgctcacaa ggatattctc cataactcaa ggctatcact gggcatttct cgttgttggg
     1561 cttgcactcc ttgggagcgg attgagcggt acctgtctgg tattcagaaa aggtttatat
     1621 aagaataagg ggatagaaca tctaagggtc ttaagtataa tatatccgct ctctatcatt
     1681 ctggcatatt tggagataac attgttacaa tttgacccgt atgtgatgcc ctggtctagg
     1741 gtgcaatttc ttttcttctt tcttaacctt acatctttcc ttgtt
//
LOCUS       DAOTKU010000365         1779 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_53654_length_1779_cov_3.073086, whole genome shotgun sequence.
ACCESSION   DAOTKU010000365 DAOTKU010000000
VERSION     DAOTKU010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53654_length_1779_cov_3.073086"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="P5310_07740"
     CDS             complement(<1..651)
                     /locus_tag="P5310_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013303422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/oligopeptide/nickel ABC transporter
                     ATP-binding protein"
                     /protein_id="HRU33286.1"
                     /translation="MKNELLRVEDLKVWFDIKRGLLSKPVYVRAVDGVSFTFNPGESI
                     SLVGESGSGKTTLGKTILRLYKPTGGKIIFEGKDITNNESGDMKWYRRETALVQQDPF
                     GALPPHFNIYRILEEPLIINNIGTKEERRDIIYRAMEEVRLTPVEDFAPKYPHMISGG
                     QMQRVVIARALIMRPKLVIADEPVSMLDASVRVEILELLRGMQEKYNTSLIYITHDL"
     gene            complement(648..1622)
                     /locus_tag="P5310_07745"
     CDS             complement(648..1622)
                     /locus_tag="P5310_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013303423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRU33287.1"
                     /translation="MDDNLLLKVQDFSLSYRTRKGDVAAVNRVNFDLKKGETLAVVGE
                     SGCGKSSLARGLIRLFPRNIKNVEGQILLNNRDILSLDEETFRKEIRWKEMAMVFQGA
                     MNSLNPVLKVVFQVAEPLIVHYDIDRNSAFGRAKEVLSMVGISGAFAERYPFELSGGM
                     KQRVIIAMALITNPKIILLDEPTSALDIITQENIMNLLKMLKKELNLSYIFITHDIAL
                     TSELADKMAVMYAGEIVELSPVEDFYSNPSHIYTRLLLDSVPTLRGEKKLKSIPGSPP
                     SLIDPPSGCKFHPRCPYVMDICKTEEPPFYKISENHFARCYLSEDKKV"
BASE COUNT          474 a          419 c          324 g          562 t
ORIGIN      
        1 aaggtcatgt gttatataga taagacttgt attatacttc tcctgcatac cccttaaaag
       61 ttcaagtatc tcaaccctta ccgatgcatc taacatagag acaggttcgt ctgcaattac
      121 cagttttggt ctcattatta gagccctggc tatgactact ctctgcatct gtccaccact
      181 tatcatatga ggatacttcg gggcaaagtc ttctacaggg gttaacctta cctcttccat
      241 cgccctgtat atgatatctc ttctctcctc tttagtacca atattattta ttatcagggg
      301 ttcttcaagg attcggtaga tattaaagtg aggtggaagt gcaccaaatg ggtcctgctg
      361 taccagggca gtctcccttc tataccactt catatcacca gattcattat ttgttatgtc
      421 ttttccttca aatataatct tgccccctgt tggtttataa agtctcaata tggtctttcc
      481 aagggtggtc tttccgctac cactctctcc aactaaagat attgactctc ccgggttgaa
      541 ggtaaaactt accccatcga ctgctctgac gtatacgggt tttgatagta atcctctctt
      601 tatatcgaac cagaccttga ggtcttccac tctgagcaat tcattcttca tactttctta
      661 tcctcactta gataacacct ggcaaagtgg ttctctgata ttttgtaaaa gggtggttct
      721 tcagtcttac atatatccat tacatacggg catctcggat ggaatttaca accggaagga
      781 gggtctatca ggcttggagg agagccagga atagatttta actttttctc tcccctcaat
      841 gtggggacgc tatcaagtaa gagcctcgta tatatatgag atgggttact gtaaaaatct
      901 tccactgggc ttagttctac aatctcccct gcatacataa cagccatctt gtctgccagt
      961 tcactggtaa gggctatatc atgagtaata aatatataac tcaggtttag ctccttctta
     1021 agcatcttca gcagattcat tatattctcc tgggtgatga tatccagtgc tgatgtgggc
     1081 tcatctaaaa gaataatctt tggatttgtg ataagagcca tagcgataat taccctctgc
     1141 ttcatcccac cagagagttc aaatggatat ctttcggcga aagccccgga tatccccacc
     1201 atactgagca cctcttttgc tcttccgaat gccgagtttc tatctatatc atagtggacg
     1261 atgaggggtt ctgccacttg aaatacgacc ttgagaacgg gatttaaaga gttcatagcc
     1321 ccctggaaga ccatcgccat ttctttccat cttatctctt ttctgaatgt ctcctcatcc
     1381 agagacaaaa tatccctgtt atttaacagt atctgtcctt ccacattttt tatatttctc
     1441 gggaagagcc tgatgagacc acgggctaaa gagcttttac cacaacctga ttcaccaact
     1501 acagccagcg tctccccttt ctttaaatca aaattaactc tatttaccgc cgctacatca
     1561 ccttttctcg ttctgtaaga gagactgaaa tcctggactt ttaaaagaag gttgtcatcc
     1621 atagtcttac agctccttca gttttggatt gacgattcta tcaaggataa agccaagcag
     1681 agagaacgag acggcggtaa gaatcagaag ggaagaggga atcaataccc agtaaaaata
     1741 tcccttatag agcgctccat tattgaacgc ttcctctat
//
LOCUS       DAOTKU010000366         1778 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_53699_length_1778_cov_3.219965, whole genome shotgun sequence.
ACCESSION   DAOTKU010000366 DAOTKU010000000
VERSION     DAOTKU010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53699_length_1778_cov_3.219965"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="P5310_07750"
     CDS             <1..358
                     /locus_tag="P5310_07750"
                     /inference="COORDINATES: protein motif:HMM:NF015436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HRU33288.1"
                     /translation="TAGLVTIEDILEELVGEIMDEYDINEEAMIERVALDEIIVDGRM
                     NLEELNEILGVELPAEETDTLAGFVYDHISHIPKPGEEFEYNGVIIRVEEVRGRRITK
                     LRIKKAIKGEEDEEVD"
     gene            342..731
                     /gene="cdd"
                     /locus_tag="P5310_07755"
     CDS             342..731
                     /gene="cdd"
                     /locus_tag="P5310_07755"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HRU33289.1"
                     /translation="MKKLIDMAKDMLDRAYAPYSHYRVGSVIETSSGRVYSGCNIENT
                     SFGLTVCAERAAIFKAISEGERAFRRIVVVSDNDSLPVPCGACLQVMSEFCNDDLEVI
                     LCSGNNNLEVYRLKDLFPKPFRLTRTE"
     gene            complement(742..1509)
                     /locus_tag="P5310_07760"
     CDS             complement(742..1509)
                     /locus_tag="P5310_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012582796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HRU33290.1"
                     /translation="MILKDKRIAIFNVANKRSIAWAIAQSVVNCGGEIIIGYQNERFK
                     ENVEELISDLSPKPLIVECDVSSDEKVEETRARIEREAGKIDGLVHSIAYAPGDALKS
                     PYMETSRDDFRVALEVSVYSFVAMSRIFSQMINPGGSIITLTYYGSEKVIPNYNVMGI
                     AKAALEASVRYLAYELGEKNIRVNAVSPGPLSTLAARGISGFVDMLQLHRERAPLRRN
                     IERREVGDTSAFLLSDLASGITGEVIYVDAGYNIMGM"
     gene            complement(1506..>1778)
                     /locus_tag="P5310_07765"
     CDS             complement(1506..>1778)
                     /locus_tag="P5310_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015042546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRU33291.1"
                     /translation="NNRKKGLSRQSVWLIVKQFASNVGLDISPHTLRHSFATHLLDNG
                     ADLRVVQELLGHSSISTTQIYTHVSTKRLKEEFEMAHPRAIEGRDV"
BASE COUNT          490 a          364 c          370 g          554 t
ORIGIN      
        1 aacagctgga ttggtgacta tagaggatat tctggaagaa ttggttggag agataatgga
       61 tgagtatgat ataaatgagg aagcgatgat agagagggta gcactagacg agattatcgt
      121 tgatggacga atgaatcttg aggaattaaa tgagatcctc ggagtagagc ttcctgctga
      181 agagacagat actctcgctg gctttgtcta tgatcatata agtcatatcc cgaaaccagg
      241 agaagagttt gaatataacg gcgttataat cagggttgaa gaggtgaggg gtagaagaat
      301 tactaaattg agaataaaaa aagctattaa aggagaagag gatgaagaag ttgattgata
      361 tggctaaaga tatgctggat agagcttacg ccccttattc acactacagg gttggttctg
      421 taatagagac ctcttcaggc agggtatact cggggtgtaa tatcgaaaat acgtccttcg
      481 gacttactgt ctgtgcagag agagctgcga tttttaaagc catctctgag ggagagagag
      541 ctttcaggag gattgttgtc gtgtcagata atgactctct ccctgttccc tgtggtgcct
      601 gtcttcaggt tatgtctgaa ttttgtaatg acgaccttga ggttatcctg tgctctggta
      661 acaataatct ggaggtatac agattgaaag acctttttcc aaaaccattc agattaacca
      721 ggacagaata gatgatggag cctacattcc cattatatta tatcctgcat ctacgtatat
      781 aacctctccc gttatacctg aagcaagatc actcagaagg aacgctgatg tatctccaac
      841 ctctcgacgt tctatatttc tcctgagtgg ggctctttcc ctgtgaagtt gtaacatatc
      901 aacaaatcct gatatacctc tggctgccag tgtacttaga ggaccaggag atacagcgtt
      961 tacccttata ttcttttctc ccagttcgta tgccaggtat cttacactgg cttctaatgc
     1021 tgccttggct atacccataa cattatagtt tggtatcacc ttttccgatc cataatatgt
     1081 gagagtgatt atacttccac ctggatttat catctggctg aatatcctcg acatagctac
     1141 aaatgagtat acgctaacct ctaaagcaac tctgaagtca tctctcgaag tttccatata
     1201 tggagacttt agtgcatcac cgggagcata ggctatagaa tgaaccagac catcaatctt
     1261 tcctgcctct ctttctatcc tagcccttgt ctcctctacc ttttcgtcac tgcttacatc
     1321 gcactctaca attaagggtt ttggggataa atcacttata agttcctcta cattttcttt
     1381 aaatctttca ttctgatacc ctataatgat ctctccacca caatttacta cagattgagc
     1441 tatcgcccag gcgatgcttc tcttattagc cacattgaat atcgctattc ttttatcctt
     1501 cagtatcata cgtctctccc ttctatggct cttggatgag ccatctcaaa ctcttcttta
     1561 agtcttttgg tagatacatg agtatatatt tgagttgttg atatacttga atgtccaagg
     1621 agttcttgaa caactctaag gtcggcacca ttgtctaaaa ggtgggtagc aaaagagtgt
     1681 ctcagggtat gagggcttat atcaagccca acgttgctgg caaattgttt cactatgagc
     1741 cacacactct gtctggatag cccttttttc ctgttatt
//
LOCUS       DAOTKU010000367         1774 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_53908_length_1774_cov_1.849331, whole genome shotgun sequence.
ACCESSION   DAOTKU010000367 DAOTKU010000000
VERSION     DAOTKU010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53908_length_1774_cov_1.849331"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="P5310_07770"
     CDS             complement(<1..224)
                     /locus_tag="P5310_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU33292.1"
                     /translation="MKRLLVGILSVVLIISSVGFVSAKTKFNVWISNTFYQRYIDVFN
                     QLHPDLEATLVFKPGIESILMTAIAGEEPP"
     gene            complement(232..1548)
                     /locus_tag="P5310_07775"
     CDS             complement(232..1548)
                     /locus_tag="P5310_07775"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRU33293.1"
                     /translation="MRHILISLLILMIMFGSFGYLYSAQEDFYSKASSKYKGTVLSLM
                     CLSGVQNRAILKLADEFQKISGIKVNVVSTPYEPIPRGSTTMVAPLENLSLHDKIAKD
                     FEKKTAKYDVILVDSLWIKEFSKNLVDLNTLFKKKEISDPKFNINDFHPTLLNSCKKG
                     NSLYGIPFLGDAMVIFYREDLFKKGGVSFPKTWAEFNKIVSSLTKKPTYGISFPGAKK
                     AELTNLFLDRYSSHLIKEDKASFNDENGIKALDMLKELIKYAQPNALEEDIQMAMERF
                     RNGEAAIFEGWISYYPDITDMFMSQVKDKVKIALVPGGKPVIGGWVAGINSDSKNKEA
                     SFLLIQYLTNVNGETKRAISLNFPTVARISTFKNKDVAKNLPFIEIIKTSLDKGITRP
                     FLPDFYLSLNNILSEAIWSALKGEDSKSALDRAASKWDDILKKGRR"
     gene            complement(1565..>1774)
                     /locus_tag="P5310_07780"
     CDS             complement(1565..>1774)
                     /locus_tag="P5310_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018752568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRU33294.1"
                     /translation="NEFMGPLIYLNDSSKYTIQLGLRMFQADFSADWSALMAMSIVAA
                     LPCIVLFFIAQRYFIQGIVLTGLKE"
BASE COUNT          559 a          372 c          267 g          576 t
ORIGIN      
        1 tctggaggtt cttccccagc tatagcagtc attaaaatgc tctctatccc tggcttgaag
       61 acaagagttg cttccagatc tggatgtaac tgattaaata catctatata cctttgataa
      121 aaggtattac ttatccagac gttgaacttt gtcttggcag atacaaaacc tacagaacta
      181 atgattaaga caacactcaa aataccaact aacaatctct tcataaatta ttcacctcct
      241 tccttttttt aaaatatcat cccactttga agctgcccta tcaagggcag actttgaatc
      301 ttcacctttc aaagcagacc agattgcttc acttagaata ttattaagac taagataaaa
      361 atctggaaga aaaggtctag taatcccttt atctagagat gtttttatta tctctataaa
      421 aggtaagttt ttcgctacat ctttattttt aaaggtggaa attctggcta cagtagggaa
      481 attaagactt atcgctcttt ttgtctcccc attaacattt gttagatact gaatcaataa
      541 aaatgatgcc tctttatttt tagaatcact atttataccg gcaacccatc cgcctataac
      601 tggcttaccc cctggaacaa gggctatctt taccttgtct ttaacctgag acataaacat
      661 atctgttata tcaggataat aacttatcca tccttcaaaa attgcagcct ctccgttcct
      721 aaatctctcc atcgccattt ggatatcctc ttctaatgcg tttggctggg catactttat
      781 caattcttta agcatgtcta aagctttaat tccattctca tcattaaaac ttgccttatc
      841 ctcttttatt aaatgagagg agtatcgatc taagaataga tttgtaagtt ctgccttttt
      901 ggctccggga aagcttatac catatgtagg ctttttggta agagaggata ctatcttgtt
      961 gaactcagcc caggtttttg gaaaagatac tccacccttc ttgaataagt cctctctgta
     1021 gaatataacc atagcatccc ctaaaaaggg tatcccataa agggaattac cctttttaca
     1081 actatttagc agggtaggat gaaagtcatt tatattgaac ttgggatcac tgatctcttt
     1141 tttcttgaag agagtattta aatctacaag atttttactg aactctttta tccacagaga
     1201 atcaaccaaa atcacatcat attttgcagt cttcttttca aagtccttag caatcttatc
     1261 gtgaagagag aggttctcaa gcggagccac catagtcgtt gagcctctag ggatgggctc
     1321 atatggggta gagaccacat taacttttat tccagaaatc ttttgaaact catctgccaa
     1381 ttttaagatt gctctattct ggactcccga aagacacatc aaactcagaa cagtgccttt
     1441 gtacttagaa ctagccttag agtaaaaatc ttcttgggca gaatataaat atccaaaact
     1501 accaaacata atcattaaaa ttaataaaga tatgagaata tgtctcatcg tttatcccct
     1561 cctatcattc ctttaatcca gtaagaacta taccctgtat aaaatatctc tgagctataa
     1621 agaacaagac tatacatggc aatgcagcta ctattgacat cgccattaaa gcagaccagt
     1681 ctgcactaaa atctgcctga aacatcctca gccctagctg tatagtatac ttggaagagt
     1741 cattaagata gattaacggt cccataaatt catt
//
LOCUS       DAOTKU010000368         1767 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_54190_length_1767_cov_3.001752, whole genome shotgun sequence.
ACCESSION   DAOTKU010000368 DAOTKU010000000
VERSION     DAOTKU010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54190_length_1767_cov_3.001752"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..781
                     /locus_tag="P5310_07785"
     CDS             <1..781
                     /locus_tag="P5310_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRU33295.1"
                     /translation="DGTDLLELPESEMRRIRGNKISMIFQEPMTSLNPVFTIGSQIAE
                     VIQLHQGLDKNSAMEKAIEMMKLVGIPSPELRAKDYPHQLSGGMRQRIMIAMALACNP
                     KLLIADEPTTALDVTIQAQILELIKELREKLGMSVLLITHNLGVVAETADNVAVMYAG
                     KIVEYGTVRDIFKNPVHPYTWGLLQAIPRLDTTRERLLTIEGSVPDMISLPPGCKFAP
                     RCMFVKNKCEISEPLLEEVTPGHMSRCWRVKEIDFSKIEVV"
     gene            783..1754
                     /locus_tag="P5310_07790"
     CDS             783..1754
                     /locus_tag="P5310_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRU33296.1"
                     /translation="MEDSILLRVEKLTKLFPIRGGIFQRTVGYVRAVENVSFSIDRGE
                     TLGLVGESGCGKTTTGRSILRLIEPTSGKVYFKGIDITALSKKDLAPLRREMQIIFQD
                     PYGSLNPRMTVGDIIGEGLEIHKIASGKEKERRIMELLEVVGLPPAYMRRYPHEFSGG
                     QRQRIGIARALAVNPKFIVCDEPVSALDVSIQSQIINLLKDLQKQFELTYLFIAHDLS
                     VVKYMSDKIAVMYLAKIVEYTTTEELFNNPKHPYTLALMSAIPVPDPDVKKQRIVLQG
                     DLPSPSNPPKGCRFNTRCPEVMSICKEVEPELKDIGKEHLVACHLWN"
BASE COUNT          590 a          311 c          410 g          456 t
ORIGIN      
        1 tgatggaaca gatctgcttg agcttcctga gagtgagatg agaaggatca gggggaacaa
       61 aatctctatg atctttcaag agcccatgac atccctcaat cctgtattca ccataggaag
      121 tcagatagct gaggtgatac aattacatca gggattggat aagaatagtg ctatggaaaa
      181 ggctatagaa atgatgaaat tggtagggat tccatcccca gaactcagag ctaaagatta
      241 cccccatcaa cttagcggag gtatgaggca gagaattatg atagctatgg ctttagcgtg
      301 taatccaaaa ttattaatag cagatgagcc aaccactgct cttgatgtca ccatacaggc
      361 gcagatactt gaactgataa aagaattgag agaaaaactg ggaatgtctg tactgcttat
      421 cactcacaat ctcggagtag tagcagaaac tgcagataat gttgcggtta tgtatgcggg
      481 aaaaatagta gagtatggga ctgtaaggga tatatttaaa aaccctgtac acccctatac
      541 ctggggatta ttacaggcta tccccaggtt agataccacc agggaaaggt tattaacaat
      601 agaaggttct gtcccagaca tgatatcact tccaccaggt tgtaaatttg ctcccagatg
      661 catgttcgtc aaaaataaat gtgagatctc tgagcctctc ctggaggagg taacaccggg
      721 acatatgtcc aggtgctgga gagtaaaaga gatagatttc agtaagatcg aggtagtata
      781 atatggaaga tagcattctt ttaagggtag aaaaacttac aaagctcttt cctataagag
      841 ggggaatttt tcagaggact gtaggttatg taagggctgt agaaaatgtt tcattttcca
      901 tagacagagg agaaacattg ggtcttgtag gagaaagtgg atgcggaaag actaccactg
      961 gtagaagtat cttacggctt atagaaccca cttctggtaa ggtttacttt aaggggattg
     1021 atataacagc actgagtaaa aaagatttag cccctctaag aagagaaatg cagattatat
     1081 ttcaggaccc atacggatct ctaaatccca gaatgaccgt tggagatatt ataggggagg
     1141 gattagaaat ccataagatc gcctcaggaa aagaaaaaga aaggcgaata atggagctat
     1201 tagaggttgt gggattacct cctgcatata tgcgaagata tcctcatgaa ttcagcggcg
     1261 gacagagaca gcgtataggt atagccagag ccttagcggt aaatccgaag tttatagtct
     1321 gcgatgaacc cgtttccgct ttagacgttt caatacagtc tcagattata aatctcttaa
     1381 aagatttaca gaaacagttt gagcttacct atctctttat agctcatgac ctcagcgtgg
     1441 taaaatatat gagtgacaag atagctgtta tgtacctggc taagatagta gagtatacaa
     1501 ccaccgaaga gttattcaat aatccaaaac atccgtatac cctggcactg atgtcggcta
     1561 tccctgttcc tgatccagat gtaaaaaaac agagaatagt acttcaggga gatcttccca
     1621 gcccaagtaa tccacctaag ggctgcagat ttaatacgag atgtcctgaa gtaatgagta
     1681 tatgtaaaga ggtagaacct gagcttaaag atataggaaa ggagcatctg gtagcatgtc
     1741 atctatggaa ttagagctaa ataatct
//
LOCUS       DAOTKU010000369         1762 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_54422_length_1762_cov_3.104277, whole genome shotgun sequence.
ACCESSION   DAOTKU010000369 DAOTKU010000000
VERSION     DAOTKU010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54422_length_1762_cov_3.104277"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..970)
                     /locus_tag="P5310_07795"
     CDS             complement(<1..970)
                     /locus_tag="P5310_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018999588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HRU33297.1"
                     /translation="MTKKETTRLDMASFTKIQIKLASPEKIKEWGTRRVGGQVIYGEV
                     KKPETINYRTFKPEMDGLFCERIFGPTRDWECYCGKYRKLRYKGIICDKCGVEITSSK
                     VRRERMGYIELAAPVAHIWFFKSVPSRIGLVLNLSMKDLEKVLYFAAYIVLDPGDTSL
                     QKCQVISDVEYEEYRDKYGNKFRAGMGAEAIRELLAEVDLESLSKELRKELETANGQK
                     RVKILKRLDMVEGLFKSGNRPEWMILERLPVVPPELRPMVPLDGGRFAVSDLNDLYRR
                     VINRNNRLKKLIELQAPEIIIRNEKRMLQEAVDALIDNGRRGAAVVG"
     gene            complement(963..>1762)
                     /locus_tag="P5310_07800"
     CDS             complement(963..>1762)
                     /locus_tag="P5310_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HRU33298.1"
                     /translation="DKLAGRHGNKGVIANILPVEDMPFLPDGTPVDVVLNPLGVPSRM
                     NLGQIFEAHTGLIANSRDFIAESPVFNGATEKEIFDSLKELMEEIPGLNDDGKCILYD
                     GRTGEKFDSPVTVGYMYIMKLIHLVDDKIHARSTGPYSLVTQQPLGGKAQFGGQRFGE
                     MEVWALEAYGASYTLQEMLTIKSDDIDGRTKTYEAIVKGKKIQTPGIPESFKVLIREL
                     RGLALDVIIYDKDGREISIDDEGERSPLEIWNEFNVNLEGMEKDGDD"
BASE COUNT          480 a          474 c          233 g          575 t
ORIGIN      
        1 aacctactac cgcagcacct cttcttccat tatcaatgag agcatctacc gcttcttgca
       61 gcattctttt ttcatttcta attattatct ctggcgcctg taattcaatc aatttcttca
      121 atctattatt tcgatttatg acccttctgt ataaatcatt cagatcactt acggcgaatc
      181 ttcccccatc aagagggacc attggtctca actctggagg gactacaggc aatcgttcca
      241 gaatcatcca ctctggtctg ttaccggatt taaataaacc ctcaaccata tcaagtctct
      301 taagaatctt aaccctcttc tgcccattag ctgtttccaa ttccttcctg agttctttag
      361 aaagtgactc taaatctacc tctgccaaaa gctctcttat agcctcagca cccatccctg
      421 ctctaaactt attaccatac ttatcacgat attcttcata ttcaacatca cttatcacct
      481 gacatttctg cagagatgta tctccagggt caagcactat ataagcagca aagtataata
      541 ctttctccaa atccttcata ctgaggttca aaaccagacc tattctggat ggtacactct
      601 tgaaaaacca tatgtgtgct accggtgcag ctaactctat ataccccatc ctctctcttc
      661 ttactttaga agatgtgatc tctaccccac atttgtcaca gatgattccc ttatatctta
      721 gcttcctgta cttcccacaa tagcactccc aatctctggt gggaccgaat atcctctcac
      781 agaaaagacc atccatctct ggcttgaagg tacgataatt tattgtttca ggctttttta
      841 cctctccata tattacctgc ccccctactc tcctagttcc ccattctttt atcttttccg
      901 gagaagctag ttttatctga atcttagtaa aactagccat atcaagtctt gtcgtttctt
      961 tcttagtcat ctccatcttt ctccatccct tccagattca cattaaactc attccaaatt
     1021 tctaagggac ttctttcacc ttcatcatca atagagattt ccctaccatc tttatcgtaa
     1081 ataataacat ccaatgccaa tcctctcagc tctcttataa gcaccttaaa tgactcagga
     1141 atacctggag tttgaatttt cttaccctta acaatcgcct cataggtctt agttcttcca
     1201 tcgatatcat cagactttat tgtaagcatt tcctgaagtg tatagcttgc cccataagct
     1261 tccagagccc atacttccat ctctccaaat ctctgcccac caaactgcgc tttaccacct
     1321 aaaggttgct gagtaaccag agaataaggt ccagttgaac gagcatgtat cttatcatca
     1381 accagatgaa tgagcttcat tatatacata tatcctacag ttacaggact atcaaatttt
     1441 tctcctgttc ttccatcata cagaatacat ttaccgtcat cattgagacc aggtatttct
     1501 tccataagtt cttttaaaga gtcgaagatc tccttctctg tcgccccatt aaatacagga
     1561 gactcagcta taaaatccct gctatttgct ataagtccag tatgagcttc aaaaatttgt
     1621 cccaggttca tcctcgaggg aactccaaga ggattcaaaa cgacatccac aggagtcccg
     1681 tctggtaaaa atggcatatc ttccacaggg agtatgttgg ctattacacc tttattacca
     1741 tgtctaccag ctaacttatc tc
//
LOCUS       DAOTKU010000370         1756 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_54709_length_1756_cov_2.667255, whole genome shotgun sequence.
ACCESSION   DAOTKU010000370 DAOTKU010000000
VERSION     DAOTKU010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54709_length_1756_cov_2.667255"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            37..570
                     /locus_tag="P5310_07805"
     CDS             37..570
                     /locus_tag="P5310_07805"
                     /inference="COORDINATES: protein motif:HMM:TIGR01668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HRU33299.1"
                     /translation="MIFSRLFKPFGAVSRVTELDIKRLLDYNIKGVIFDIDNTLIPWG
                     SWDIPEDTRIFIEELKKIDIKCCLLSNTDKRDRAVFIGKELGLAVIPGALKPLPFGFL
                     KALKILGLKREDVVSVGDQLFMDVLGSNMVRIRPILVKPLTDRDFITTRLLRLFEKII
                     LPEVTKEVENFGYIKSG"
     gene            548..760
                     /locus_tag="P5310_07810"
     CDS             548..760
                     /locus_tag="P5310_07810"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF013365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HRU33300.1"
                     /translation="MDISNLDKILTITHNRYVLTIAVAQRAEEIFNTATLLTRRLNPV
                     SIAIRELAEDKLKIRLPGFLEEDKTE"
     gene            775..862
                     /locus_tag="P5310_07815"
     tRNA            775..862
                     /locus_tag="P5310_07815"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:809..811,aa:Ser,seq:cga)
     gene            899..1705
                     /locus_tag="P5310_07820"
     CDS             899..1705
                     /locus_tag="P5310_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012582599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HRU33301.1"
                     /translation="MIDYHMHLEKGPFSTEWWEKFYQREVENGIEECGISEHAHRFRE
                     ARGIYDHLPYTEKWCDSSLEEYVDFVTQLKKDYPVKLGIEMDYLPEKESEIKDFLMRY
                     DVFDYVLGSVHWLQSGFGFDIDPNDPRWSNDLEEIFFDYFDRVKRAIHSGLFDIIAHI
                     DLIKLWGVPLPAHIDDIYMDMGRIISASNAALEINTSGLRRPVKEIYPSPRFLKTISQ
                     FDVPITFGSDAHLPEDAGRFIRDACTLAKELGFKTILTFNKRKPTPVLLT"
BASE COUNT          521 a          296 c          420 g          519 t
ORIGIN      
        1 aggctctgtt gaggcggagt ttcaagtaaa acaacagtga ttttttctcg tctctttaag
       61 ccttttggcg ctgtgtccag agtgacagaa cttgatatta aaagattgtt ggactataat
      121 atcaaagggg taatatttga tatagacaat acccttattc cgtggggttc ctgggatata
      181 cctgaggata ccagaatttt tatagaagaa ttaaagaaga tagatattaa atgttgtctt
      241 ttatctaata ctgataagag agatagggct gtatttattg gtaaggagct tggattggca
      301 gtaataccag gtgcacttaa gccgttacca tttggttttc ttaaagccct gaagatactt
      361 gggctgaaaa gagaagatgt agtatctgta ggtgatcagt tatttatgga tgttttggga
      421 agtaatatgg ttcgaattag acctatactg gttaaaccgt taaccgatag agattttatt
      481 acaaccagat tattgaggct atttgagaag ataattttac ctgaggtaac taaggaggtt
      541 gagaattttg gatatatcaa atctggataa gatattaacc ataactcaca atagatatgt
      601 actgacgatt gctgtagccc agagagcaga ggagatattc aatactgcta ctctacttac
      661 caggaggttg aaccctgtaa gtatcgctat aagagagctg gcagaggata agcttaaaat
      721 cagactccct gggtttttgg aagaggataa gacagagtaa tactcatatc tggtggagag
      781 gtggctgagt ggattaaggc actcgcctcg aaagcgagcg ggtagaaata ccccgtgggt
      841 tcgaatccca ccctctccgc cataaatttt taaatgaatg gaggattaaa ctcggttgat
      901 gatagactat catatgcatc tggagaaagg tccgttttct acagaatggt gggagaagtt
      961 ttatcagaga gaggttgaaa atgggataga agaatgtgga ataagtgaac atgcccatag
     1021 gtttagggag gctagaggta tatatgatca tctcccctat accgagaagt ggtgtgatag
     1081 ctcactggaa gaatatgtgg atttcgtcac tcaactgaaa aaggattatc cagtgaagct
     1141 tgggatagaa atggattatc ttcctgagaa agagtctgag attaaggatt ttctcatgcg
     1201 ttatgatgta tttgattatg ttttgggatc ggtccactgg cttcagagcg ggtttggctt
     1261 tgatatagac ccgaacgatc cgagatggag caatgatctg gaagagatat tttttgatta
     1321 ctttgacagg gtaaaaaggg ctatccacag tggattattt gatattattg cccatataga
     1381 cctgataaag ctctggggag tgcctctccc agctcatata gatgatatct atatggatat
     1441 gggcaggatt atatccgcct ccaatgcagc cctggagata aatacttcag gtctgaggag
     1501 acctgttaaa gagatatacc catcgccaag attccttaag actatctctc agtttgatgt
     1561 acccataaca tttggctctg atgctcacct ccctgaagat gcagggaggt ttataagaga
     1621 tgcctgtact ctggcaaaag aacttgggtt taagacaatc ctgaccttta ataagagaaa
     1681 gcctaccccg gtattactga cttagttttc agatctttcc ctcgtatcct tttcctcccc
     1741 ccccctctcc gggagg
//
LOCUS       DAOTKU010000371         1745 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_55203_length_1745_cov_3.328994, whole genome shotgun sequence.
ACCESSION   DAOTKU010000371 DAOTKU010000000
VERSION     DAOTKU010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55203_length_1745_cov_3.328994"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            256..>1745
                     /locus_tag="P5310_07825"
     CDS             256..>1745
                     /locus_tag="P5310_07825"
                     /inference="COORDINATES: protein motif:HMM:NF039707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6259 domain-containing protein"
                     /protein_id="HRU33302.1"
                     /translation="MSDVYFFDTNYLIFGNSCFEMALERDGRLRWIINKKTEENYVKN
                     HYNRWILKILDSDKKEFNLGKPDYFWQDEELCIYYKNQLEVEIRIKVLPDNPESIWTL
                     RVKNSSSEEIMDIIFPHISGIYLGDNWRDNYLVYPYIAGERIENPVETFAMAPSKILW
                     DWLDYRYVYIVDGVSTTRDNELYVRELPYSGPVSMSWMDYYNRNAGFYMASYDEDKTL
                     TYVRAETPGPDCPCMGFNFRKRLEISSGEEKSFTYGIGIHTGDWHWGADRYREWFYSV
                     FPKRKESPDWLLNSSGLIAHYDFRYQNGSSFHSFDDIPALFDKTKEQGMDHLFISGWN
                     EGGFDNGYPLYTPDKELGGEEIFKSKIDEVNNKGGHITTYINVRIVNTSYIDRIPEGI
                     LTNHMERYGAVDFYVVDPSSDRWHNLILETVKNLVDYGVSGVYLDELAMAGAFQDIPD
                     WIRGYTAMLDKIVERHPDKVFIIEGCSDMYGRYVDLQLVSTFFYWYT"
BASE COUNT          568 a          263 c          404 g          510 t
ORIGIN      
        1 ctcagtacag aaaatgtgtg gagtataggg acagtgggtc tggctcctac acccctggta
       61 attagaaaga acttcaagaa tgttccgcag aagagcttct acgaatggct cttgcttcaa
      121 atatctaata caagtccaga gcagtacttt ataaaggaat aggttattat ctctatccgg
      181 caagaggagt acagaactcc gtacccctct tactttgttc tttttgataa taagagagta
      241 aaaaaggaga acagaatgtc tgatgtatac ttttttgata ctaactattt aatctttgga
      301 aattcctgtt ttgagatggc acttgaaaga gatggcagat taagatggat tataaacaaa
      361 aagaccgaag agaattatgt aaagaatcac tataataggt ggatcctgaa gatactcgat
      421 agtgataaaa aagaattcaa tcttggtaag cctgattatt tctggcagga tgaggagctc
      481 tgcatctatt ataagaatca gctggaagtt gaaataagaa taaaagtcct cccggataat
      541 cctgaaagta tatggacatt aagagtaaag aattcttctt cagaggagat tatggatatt
      601 attttccccc atatatcagg aatatatctg ggtgataact ggagagataa ctatcttgta
      661 tatccataca tcgccgggga aaggatagaa aatcctgtgg agacatttgc tatggcacct
      721 tccaagatac tctgggactg gttagattat agatatgtct atattgtgga tggagtatct
      781 acaacgagag ataatgaact ttatgttaga gaattaccat actctggtcc agtatcaatg
      841 agctggatgg actattataa cagaaatgct ggattttata tggcttctta tgatgaagat
      901 aaaactctta catatgtaag agcagagact cctggaccag actgtccctg tatgggattt
      961 aacttcagga agagactgga gatatcctct ggagaggaga aaagttttac ctacggtata
     1021 ggaatacata ccggtgactg gcattggggt gcagacagat atagagaatg gttttattcg
     1081 gtttttccaa aaaggaaaga gtctcctgac tggctattaa atagtagtgg tttgatagct
     1141 cactacgact tcaggtacca gaatggaagt agctttcaca gtttcgatga tattccggct
     1201 ctttttgata agactaaaga acagggaatg gaccaccttt ttatatcagg atggaatgag
     1261 ggaggttttg ataatggtta cccgctgtat acccctgata aggagttggg aggggaagag
     1321 atattcaaaa gtaaaattga tgaggtaaat aataaagggg gacatatcac aacttatata
     1381 aatgtaagga tagtgaatac ctcctatata gatcgaatcc cggaagggat attaaccaac
     1441 catatggaaa gatatggtgc tgtggatttt tatgtggttg acccctcttc tgacaggtgg
     1501 cacaatctta tattagagac tgtaaaaaat ttggtagatt atggagtatc tggcgtatat
     1561 cttgatgagt tagctatggc aggagccttt caggatatcc cggattggat cagaggatac
     1621 actgcgatgt tagataagat agtggaaaga catccggata aggtattcat tatagaaggt
     1681 tgcagcgata tgtatggaag atatgttgat cttcaactgg tctcaacgtt cttttactgg
     1741 tatac
//
LOCUS       DAOTKU010000372         1742 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_55361_length_1742_cov_2.737996, whole genome shotgun sequence.
ACCESSION   DAOTKU010000372 DAOTKU010000000
VERSION     DAOTKU010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55361_length_1742_cov_2.737996"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1202)
                     /locus_tag="P5310_07830"
     CDS             complement(<1..1202)
                     /locus_tag="P5310_07830"
                     /inference="COORDINATES: protein motif:HMM:NF013444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRU33303.1"
                     /translation="MLQDETPYLKAIFDYIEKKPVRFHYPGHKGNLISLDLTEIPQTD
                     NLFIPEGPLLKAQELSADAFGADYSYFLVNGSTQGIHSAFLSSFKDGDKVLVPFFSHR
                     SIIEGIILTGVKPIFIGEEIDRWGIPQNVRLEGLDRCDNGVKGIVITNPNYFGLTPEL
                     KEIVEYAHNRDMIVIVDEAHGAHLHFNKKLPPSGLDAGADIVIQSMHKMGISLTQGAM
                     IHLKGERIDKDILRENVLLLSTTSPSTIVLGSIDLSRRELAINGERILDNLLSEIEKL
                     SYKIKNTGFSMYEKPGMDRTKLTLYNLDGDKLSKKLWEDFKIQVEMSSGTYCLFILSL
                     LDSKSTLNTLLSALKEIEHFPARNFIPPPGYELIKSPKMAYFSLKEKIKLTSAVGRIS
                     GEIITRYPP"
     gene            complement(1208..>1742)
                     /locus_tag="P5310_07835"
     CDS             complement(1208..>1742)
                     /locus_tag="P5310_07835"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HRU33304.1"
                     /translation="GVGCLSFIVIDRQPDFKRAILVDIQEEPFRVLKRNIEENMLQER
                     VTLVNRDARELPVLFPKMSNSFDVVFTNPPHIVRGRGRLSPYTGKAISNTELLLALSD
                     IAHIASYFLKIKGRLYMIHRSERLGEIIKELDDFNLEVKRLCFVHTGKIRPSKRVLVE
                     ARKGGRPGIRVEPPIFL"
BASE COUNT          486 a          380 c          308 g          568 t
ORIGIN      
        1 gggggatatc tggtaatgat ctctcctgag attcttccaa ctgcagatgt cagttttatc
       61 ttttctttaa gagaaaagta agccattttt ggtgatttta tcagctcata tcctggtggg
      121 ggaataaaat ttctggcagg gaaatgttct atctctttta atgcactaag aagagtattt
      181 agagtgcttt ttgaatcgag taaagacagg ataaataagc agtaagtccc ggatgacatc
      241 tctacctgaa tcttgaaatc ctcccatagt ttcttagaaa gcttatcccc atccagattg
      301 tatagagtta acttggtcct atccatacca ggtttctcat acatagaaaa tccagtattc
      361 tttatcttat aacttaactt ttctatctca gagagtaaat tatccagtat tctttccccg
      421 ttaatggcaa gttctcttcg agataggtct atagaaccca ggacaatggt ggatggactg
      481 gtggtggaga ggagtagaac attctcccgt aatatatcct tatctatcct ttcccccttc
      541 agatgtatca tggcaccctg agttagagat atacccattt tatgcatgct ctgaattact
      601 atatctgccc ctgcatctaa cccggaagga ggtagctttt tattaaagtg aagatgggca
      661 ccatgtgcct catctactat aactatcata tctctattat gggcatattc gacgatctct
      721 tttaactctg gcgtcagacc aaagtagtta ggattagtta ttactatacc cttaactcca
      781 ttatcacatc tatctaaacc ttccagtctc acattctgag gaattcccca ccggtctatc
      841 tcttctccaa taaatattgg ctttacgccg gtaagaatta tcccctctat aatactcctg
      901 tgggagaaga atggaacaag aactttatct ccatctttga aagagctgag aaatgcggaa
      961 tgaattccct gggtacttcc atttacaaga aaatagctgt agtctgcccc gaaggcatct
     1021 gcagaaagct cctgagcttt taagagagga ccttctggga taaatagatt atctgtctgg
     1081 gggatttctg tcaggtctaa ggagataaga ttccccttat gacctggata gtggaatctt
     1141 accggtttct tttcaatata atcaaagatg gcttttaaat aaggagtctc atcctgtaac
     1201 ataggtttta taaaaatatg ggaggttcga ccctgatccc tggtcttcct ccctttctag
     1261 cttctaccag taccctctta ctcggtcgta tcttgccggt atggacaaaa catagccttt
     1321 ttacttccag gttaaaatcg tccagctctt ttattatttc ccccaacctt tcgctcctgt
     1381 gtatcatata taaccttccc tttatcttca aaaaataact ggctatatga gcgatatcac
     1441 tcagagcaag taagagttcg gtattgctga tagctttccc tgtataaggg cttaaccgac
     1501 ctctccccct tactatatgt gggggatttg taaataccac atcaaaggaa tttgacatct
     1561 ttggaaataa tacaggtaac tctctggcat ctctatttac cagagttact ctctcttgaa
     1621 gcatattttc ttctatattt ctcttcagta ccctgaatgg ctcttcctga atatcaacca
     1681 gtatagctct tttaaaatca ggttgtctat caataactat aaaagaaagg caaccaaccc
     1741 ca
//
LOCUS       DAOTKU010000373         1740 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_55455_length_1740_cov_3.193472, whole genome shotgun sequence.
ACCESSION   DAOTKU010000373 DAOTKU010000000
VERSION     DAOTKU010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55455_length_1740_cov_3.193472"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..981
                     /locus_tag="P5310_07840"
     CDS             <1..981
                     /locus_tag="P5310_07840"
                     /inference="COORDINATES: protein motif:HMM:NF039831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5693 family protein"
                     /protein_id="HRU33305.1"
                     /translation="TGEDSIIEENLQLITSIKAELEKEGFNLGKASFFEKWGGFRFAN
                     ISMGIGIASVILLIGFDILPYSVLRILVVISLLAGLGTTILPFTIFRKLLALGIASIF
                     PILPIWLVLFNGREEKVLYKIIKIALLGIIGGVLVGGGLSSTDFMLQINQFMGVKVAH
                     IIPFILLFIVIYLKDREPVNNILIYPLNILSFLVILLLAGGALFYIARTGNISSEAVW
                     GFELKMRSALEQLMLVRPRTQEFLIGYPAIFLAIEFLKTKRELGIIFGFVSLITPISI
                     INSFCHIHTPIILTLFRTFNGFILGCIVGYLVLLIVRELLKFISSHTFST"
     gene            complement(915..1508)
                     /gene="coaE"
                     /locus_tag="P5310_07845"
     CDS             complement(915..1508)
                     /gene="coaE"
                     /locus_tag="P5310_07845"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF013301.2"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HRU33306.1"
                     /translation="MSEITIVVTGTIGSGKSTVCGFFQELGAEYISSDDIAKRLIRED
                     DRVKRLIGYFFSDYGSRDFVKKIFSDTSKRLTLNSITHPFVLGYLRDYKKSSKKVLVI
                     EVPLYIEIRSWDIGDITVVTYAPEDVLLRRIMEKWNIDLEEAEERLNSQLPQEIKLLF
                     GDYKIDTSISLDFTRAQVEKVWEEINLSSSRTISRTR"
     gene            complement(1496..>1740)
                     /locus_tag="P5310_07850"
     CDS             complement(1496..>1740)
                     /locus_tag="P5310_07850"
                     /inference="COORDINATES: protein motif:HMM:NF018531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HRU33307.1"
                     /translation="QSLSRDEVESLYKSIKEILEEALKLGGTSIEEFTNLISGERGFE
                     PKVHRREGEPCEVCETPIVMIKIGGRGTYYCPKCQR"
BASE COUNT          451 a          351 c          314 g          624 t
ORIGIN      
        1 actggagagg attctattat agaggaaaat ttacagctaa taacctctat aaaggctgag
       61 ttagagaagg aaggttttaa tttaggaaaa gccagcttct ttgagaagtg gggtggtttc
      121 aggtttgcca acatatctat ggggattggt atagccagtg taatacttct gataggattt
      181 gacatcttac cctattctgt acttcgtata ctagttgtga tatctttact tgcaggtctg
      241 ggaactacca tcttaccatt tactattttt agaaaactcc ttgcgcttgg gatagcatcc
      301 atattcccga tacttcctat ctggctggtt ttatttaatg gtagagagga aaaagtttta
      361 tataaaatta taaagatagc cttgctgggt attattggtg gagttctggt aggtggtggc
      421 ttatcatcaa cggattttat gcttcagata aatcagttta tgggggtaaa ggttgctcac
      481 atcatcccct ttatacttct ctttattgta atatatctta aagatagaga gcctgttaat
      541 aatatactga tttatccgtt aaacatcttg agttttctgg ttatacttct tcttgctggg
      601 ggagctctgt tctatatagc caggacagga aatatatcat cagaggcagt ttggggattt
      661 gagttgaaga tgaggtctgc ccttgagcaa cttatgcttg taagacccag gacacaggaa
      721 tttcttatag gctatccggc gatctttctt gcgatagagt ttttgaagac aaaaagagag
      781 ttaggaatca tatttggctt tgtctctctc ataaccccaa tatctattat aaactctttc
      841 tgtcatatac atacccctat tatattgaca cttttccgta cattcaatgg atttattctt
      901 ggttgtatag ttggttatct ggtcctgctt atagtccgag agctgctcaa gtttatctct
      961 tcccatacct tctcaacctg agccctggta aaatccagac ttatagaggt gtctatctta
     1021 taatctccaa ataggagctt tatctcctga ggtagctgtg agttaagtct ttcttctgcc
     1081 tcttccaggt ctatattcca tttttccata atcctcctta acagaacatc ttctggtgcg
     1141 tatgtgacta ctgttatatc tcctatgtcc cagcttctta tctctatata tagcggtacc
     1201 tctataacca gaactttttt tgagcttttc ttataatctc tcagataacc caggacaaac
     1261 ggatgggtta tggagttaag tgtaagcctt ttactggtat ctgagaatat cttctttaca
     1321 aaatctctag agccatagtc agagaagaaa tatcctataa gtctttttac cctatcatcc
     1381 tctctgataa gtctttttgc tatatcatcg cttgatatgt attcggctcc aagctcctga
     1441 aagaaaccac atactgtact cttaccactt cctatggtgc ctgttaccac tatagttatc
     1501 tctgacattt aggacagtaa tatgtccccc ttcctcctat ttttatcata actattggtg
     1561 tctcacaaac ctcgcagggt tctccttctc tcctgtgaac ctttggttca aaacccctct
     1621 ctccggatat aagatttgta aattcttcta tggaagttcc tccaagtttt aaggcttctt
     1681 ccaggatttc ttttattgat ttatacagag actctacctc atccctgctt aaactctggc
//
LOCUS       DAOTKU010000374         1737 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_55611_length_1737_cov_2.296671, whole genome shotgun sequence.
ACCESSION   DAOTKU010000374 DAOTKU010000000
VERSION     DAOTKU010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55611_length_1737_cov_2.296671"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..832
                     /locus_tag="P5310_07855"
     CDS             <1..832
                     /locus_tag="P5310_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase RNA specificity
                     domain-containing protein"
                     /protein_id="HRU33308.1"
                     /translation="IPSFVVDRAQRVIYELKLLKEAGILPGNFPMYFDSPMGKKVTDI
                     YRKYSNLLSGELQRYYLNSEDPFDIENLHYLVSVDDSKKINDLDRGIVIAGSGMCTGV
                     RILHHLKHNIWKENTHIIFVGYQAYGTLGREIINGVKTANIMGEEIAIKAKIHTINGF
                     SAHADQNDLLSWAEGFQNRPMFIITHGELNSSKILSEVFRLKGYETFIPVRGQSIDLV
                     KREVSSVKVAPFIDLEPLLVELDREFFNLKAKLEDSSIPVEKLGVVQAILLLLKEVNK
                     "
     gene            834..1391
                     /locus_tag="P5310_07860"
     CDS             834..1391
                     /locus_tag="P5310_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HRU33309.1"
                     /translation="MDMPSILLIALSLAMDAFSVSVTSGITGGKIRYFNVLKMGFSFG
                     LFQFFMPVLGWLAGSTFRIYIVTIDHWIAFSLLSFVGVRMIWESLKDEHQTIDPFDNK
                     TLLLLSISTSIDAFAVGISFSFLDVSIFFPVLIIGIITFLLSSAGILMGNGLRHFFEK
                     FNVEIIGGLILIGIGLKILIEHLIE"
     gene            complement(1427..>1737)
                     /locus_tag="P5310_07865"
     CDS             complement(1427..>1737)
                     /locus_tag="P5310_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008411268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HRU33310.1"
                     /translation="KTGVEIVKKALEEPLKQLAFNAGLEGSVVVEKVKTLQKGHGLNV
                     MTAQYVDMIQSGIIDPVKVTRSALQNAASIAAMLLTTEAVVVEKPEEKKETPPPPPEY
                     "
BASE COUNT          500 a          294 c          372 g          571 t
ORIGIN      
        1 tattccctcc tttgtggtgg atagagctca gagggttata tacgagttga agctattgaa
       61 agaagcgggt atcctgcctg gtaatttccc tatgtatttc gatagcccga tgggcaaaaa
      121 agttacagat atttatagaa agtacagtaa tcttctatct ggcgaattgc agagatatta
      181 tttaaattct gaagacccgt ttgacattga aaatctacac taccttgttt ctgtggatga
      241 ttcaaagaaa ataaatgacc tggacagggg aatagtcata gccggaagtg ggatgtgtac
      301 aggagttaga atactccatc atttgaaaca taatatatgg aaggagaata cccatataat
      361 ttttgttgga tatcaggcgt atggaactct gggtagagag attataaatg gggtcaaaac
      421 cgccaatatt atgggggaag aaatagcgat aaaggctaag atccatacga taaatggatt
      481 ttctgcccat gcagaccaga atgatctttt aagttgggct gaaggatttc agaatagacc
      541 aatgtttata ataactcatg gagagttaaa ctcttctaaa attctttctg aagtattcag
      601 gcttaaaggt tatgagacct ttatccctgt tagaggacag agtattgatt tagtaaaaag
      661 agaggtttca agtgttaagg ttgctccatt catagatctg gaacctcttc ttgtcgaatt
      721 ggacagagag ttttttaacc tgaaagcaaa attagaggat tcttctatac ctgtggagaa
      781 attgggtgtg gtgcaggcaa tactgctctt actaaaagag gtcaataagt aagatggata
      841 tgccaagtat actccttata gcactcagcc ttgctatgga tgcttttagt gtttctgtta
      901 caagtggaat aactggaggg aagataagat attttaatgt tttaaagatg ggattttctt
      961 ttggtctatt ccagtttttt atgcctgtat tgggctggct ggcaggatcg acctttagaa
     1021 tttatattgt tacaatagac cactggatag ccttctctct tttatctttt gtcggagtaa
     1081 ggatgatatg ggagtctcta aaagatgaac atcagacaat agacccattt gataataaaa
     1141 ccctgcttct actctccatc tctacaagca tagatgcttt tgctgtaggt ataagttttt
     1201 cgttcctgga tgtttctatc tttttcccgg tattaataat aggtataata acctttctgt
     1261 tatcctcagc cggaattttg atgggtaatg gattgagaca tttctttgag aaatttaatg
     1321 tagaaattat tggtggattg atattaatag gtatcggatt aaagatactg attgagcact
     1381 taatagaata ataatagggg caccttctgg tgcccctata tttcagttag tactcaggag
     1441 gaggtggtgg tgtttctttt ttctcctctg gtttttccac tactaccgcc tcagtggtga
     1501 gcaacatagc ggctatactt gcggcgttct gtagtgcact acgtgtaacc tttaccgggt
     1561 cgataatccc agattgaatc atatccacat attgtgcggt cattacattt agcccatgac
     1621 ccttttgtaa ggtctttact ttttctacta caacagaacc ctcaagcccg gcgttaaaag
     1681 cgagctgttt cagtggttcc tctaaagcct tcttaacaat ctcaactcct gtcttct
//
LOCUS       DAOTKU010000375         1735 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_55715_length_1735_cov_2.558929, whole genome shotgun sequence.
ACCESSION   DAOTKU010000375 DAOTKU010000000
VERSION     DAOTKU010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55715_length_1735_cov_2.558929"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="P5310_07870"
     CDS             <1..343
                     /locus_tag="P5310_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012957014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU33311.1"
                     /translation="LKLVKEPSNMYDYEAIRVECAGIGKIGYVANSASTVVKGTYSAG
                     RLYDKIKDESYCRILFITKNEAIGEVVENPNEDIEAEFKKLEEYVKNTISYYDKEGIN
                     LLEDKSEPVKI"
     gene            complement(539..1570)
                     /locus_tag="P5310_07875"
                     /pseudo
     CDS             complement(539..1570)
                     /locus_tag="P5310_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495581.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
     gene            complement(1548..>1735)
                     /locus_tag="P5310_07880"
     CDS             complement(1548..>1735)
                     /locus_tag="P5310_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase"
                     /protein_id="HRU33312.1"
                     /translation="CDTSRIQDGDELEIDLENGVVRDLTGGFEMGINPLPPFMMKLLQ
                     DGGLVEHFKKYGGFSIG"
BASE COUNT          491 a          402 c          306 g          536 t
ORIGIN      
        1 tttgaaactg gtaaaagaac cgagtaatat gtatgactac gaagctataa gagttgaatg
       61 tgccggaatc ggaaagatag gttatgtggc aaatagtgca agcactgtag tcaaggggac
      121 atacagtgca ggtagactct atgataaaat taaagatgag tcatactgca ggatcctatt
      181 cataacaaag aatgaagcta taggagaggt tgtagaaaat cctaatgagg atatagaggc
      241 agaatttaaa aaacttgaag aatacgtaaa aaatactatc tcttattacg ataaggaagg
      301 tataaaccta ctggaggata aatcagaacc tgttaagata taaaaggata aagttttaat
      361 cagctctcct agttacatct cctgttccct cattaaaaag ctgtcctagc ggtgatttag
      421 agatataaag tttgaactca tatggtccaa ctaagtgggt tacaatctta gacaaaaaat
      481 taaaaaggga ggaagattcc tcccttttaa aaagccaagt tctataaaga ttagacacct
      541 atactttgag tagcttttct gctattctcc ttcccatctc tacagtggat gcactgcctc
      601 ccaggtcata cgttacatat ttaccctctt ttatggtctc agctactgca tccactatac
      661 gtttctccca atctttttct ccaagatagc caagcatcat tgcagatgcc aatattaccg
      721 ctgttggatt caccttatac tgtcccttat actttggagc agagccatgg acagcttcaa
      781 acagggcata atctcttcct atattagctc ctggtgccaa tcctaatccg cctacaagtc
      841 cagcgcagag gtctgatagt atgtcgccaa agagatttgt ggtaacaatc acatcaaaat
      901 tctctggatt ctttaccagt tgcatagcca tattatcaac gatcctgtca tcaaattcta
      961 tatctgggaa atcccctgca acctctcttg cgatatctaa aaagagtcca tcggtaaact
     1021 tcattatatt tgccttgtgc acagcggtaa ctttcttcct tccattttct ctggcctaat
     1081 tgaaagcaaa tctgactatt ctttcgcttg cctttctggt tatgattctt atactttcgg
     1141 cacaggtttt atcctcgtcc acgtagtgtt caaccccagc atacagatct tctgtattct
     1201 cccttacaac caccagatct atattgttga atcggcttgg agcaccctcg aaagatctca
     1261 ccggtcttac gtttgcaaag agtccaagct cctgcctcag ggcaacgttt acacttctaa
     1321 aacctgttcc tacaggtgtg gtgatgggac cttttaatgc tactttattc ctcttgatgc
     1381 tctctatggt tgctgctggt attggtgtcc caaatttctc cagggcttct tctcccgcta
     1441 cgcattcctc ccattcgata tccagccctg tattattcag gaccagcatg gtggcttcta
     1501 taatctcagg tccggttcca tcccccttta ttaatgatat tctgtattta gccaattgag
     1561 aaccctccat attttttaaa gtgttctaca agtccaccgt cctggagtag tttcatcata
     1621 aatggtggaa gtggatttat acccatctca aaaccacctg ttaaatctct gaccacccca
     1681 ttctcaaggt ctatctccag ctcatcacca tcctgtatcc tggaagtatc acact
//
LOCUS       DAOTKU010000376         1728 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_56000_length_1728_cov_4.074716, whole genome shotgun sequence.
ACCESSION   DAOTKU010000376 DAOTKU010000000
VERSION     DAOTKU010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56000_length_1728_cov_4.074716"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /locus_tag="P5310_07885"
     CDS             <1..659
                     /locus_tag="P5310_07885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HRU33313.1"
                     /translation="VLNGIFGYNIPLGILPVGRGNDFFRTISNSRILDVILKPFLDIP
                     KFNMVDVGRMNDRYFINVAGVGFDAMVAGISEPVRFLGPVAYLGSALVGLLKNKGIRL
                     KLNIDGNKIDMNALMIAIANGRYFGGKMLIAPEASPWDGKFDVCLIENASKLEVLKTL
                     PKVYSGKHIYHPKVKTFKAEYLEITSTGNMPVEMDGEIFYTDRLKIEMIPRSVPLILS
                     "
     gene            714..1037
                     /locus_tag="P5310_07890"
     CDS             714..1037
                     /locus_tag="P5310_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU33314.1"
                     /translation="MKSLLGKVALILVLVFSIQVLTGTFAMAAQAQTAKLDPLLAALA
                     SFILPGLGQFLLGNQARAINHVLIYLGIWIVGSLLSPYTYGVTAILPLVWAIYSAYDA
                     YQMAR"
     gene            complement(1055..1372)
                     /locus_tag="P5310_07895"
     CDS             complement(1055..1372)
                     /locus_tag="P5310_07895"
                     /inference="COORDINATES: protein motif:HMM:NF018685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HRU33315.1"
                     /translation="MIIFLVYMFLKLDNSSGETILIQNYEGNFIRLPLSEDKTIEIPG
                     PLGRSVVEIKNRRVRILSSPCPDKLCVKQGYISESGQVIICLPNRVVIKIEGRASFDA
                     LTY"
     gene            complement(1463..>1728)
                     /locus_tag="P5310_07900"
     CDS             complement(1463..>1728)
                     /locus_tag="P5310_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU33316.1"
                     /translation="TLKAQIDSDPDTNRSQSFVDAKVKLTDNVSLIGAHYSYTNGQTA
                     WAAGVNYSYNWSDNADLSVTLKHLEDPFDTPADVFSASVSTKF"
BASE COUNT          520 a          306 c          381 g          521 t
ORIGIN      
        1 aggttctaaa tggtatattt ggatataata tccctcttgg aatactccct gtaggaagag
       61 gcaatgactt cttccgtact ataagtaatt ccaggatact ggatgttatc cttaaaccgt
      121 ttctcgatat acctaaattc aatatggtgg atgtgggcag gatgaatgat aggtacttta
      181 taaatgtggc aggggtcggt tttgatgcta tggttgcagg tatctcagaa ccggtaagat
      241 ttctgggacc tgtggcatat ctgggttcag ccctggttgg gctgctgaag aataaaggga
      301 taaggcttaa actgaatatt gatggtaaca agatcgatat gaatgcatta atgatagcca
      361 tagctaatgg gagatacttc ggtggcaaga tgttgatagc accagaggca agcccctggg
      421 atgggaaatt tgatgtatgt ctgatagaga atgcatctaa actggaggtc ttgaagacac
      481 tacccaaagt atacagtggt aaacatatat atcatccaaa ggtaaagaca tttaaagcag
      541 agtatctgga gattacatct accgggaata tgccggtaga gatggatggt gaaatctttt
      601 atactgatag gctaaaaata gaaatgattc caagaagtgt acccttgatt ttaagttaat
      661 tctctgataa gataagagat gtataattta ggaaagaaag gaggtggaaa aggatgaagt
      721 ctctattagg aaaggtcgct ctcattctgg tcttggtctt ttctatccag gttcttacag
      781 ggacatttgc catggctgct caggcacaga ctgcaaaatt agatcctctt ctggcagcat
      841 tggcgtcttt tattctcccg ggtctggggc agtttctact cggtaatcag gccagagcta
      901 ttaatcatgt attaatttat ttagggattt ggatagtagg atctcttctg tctccttata
      961 catacggcgt tactgctatc ctacctctag tatgggcaat ctactctgct tacgatgctt
     1021 atcagatggc tcgataaaat aaggaattct taagctagta ggtgagagca tcaaaagatg
     1081 ctctcccttc tatttttatt accactctat tgggcaagca tattataacc tgaccagatt
     1141 cacttatata tccctgtttg acacataatt tatccgggca gggagaggat aaaattctta
     1201 cccttctatt ttttatctct accacactcc tacccagagg tcctgggatt tcgattgttt
     1261 tatcctctga gagggggagg cgtataaaat tcccttcata attttgaata aggattgtct
     1321 cccctgaaga gttatccagt tttaaaaaca tatataccag aaaaattatc aaaaaaagaa
     1381 taaaaaataa taaaaaatct ttcttcttca taatacaaaa taaaaagccc ccgggaatcc
     1441 ccgggggctg gaatatagtc tattagaact ttgtagatac agaggcggag aagacatcag
     1501 ctggggtatc aaatggatcc tcaagatgtt tgagtgttac agaaagatca gcgttgtcgc
     1561 tccaattata ggaataatta acccctgcag cccaagcggt ttgtccgttg gtatagctat
     1621 agtgggcacc gataagtgat acattgtcag taagtttaac cttagcatcg acaaaagact
     1681 gactcctatt agtatctggg tccgaatcaa tctgggcttt tagtgtta
//
LOCUS       DAOTKU010000377         1727 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_56047_length_1727_cov_4.313397, whole genome shotgun sequence.
ACCESSION   DAOTKU010000377 DAOTKU010000000
VERSION     DAOTKU010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56047_length_1727_cov_4.313397"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /locus_tag="P5310_07905"
     CDS             <1..816
                     /locus_tag="P5310_07905"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRU33317.1"
                     /translation="PEGINGLKVEDASVVAIWGFERKRTEEVASAGKIPEIVSYPEDM
                     IGKVDAVMVVLRDGAFHYKFSKPFIEAGIPTFIDKPIETSIDNAKRIIELANEKKTPI
                     TSFSTLRYDEPIVKFKEDLSSKGPLTTGFVCGPADATSEYQGIFFYGVHIVELCQEIF
                     GSGIERVMAVNHHNNIAITLTYSDEKIIELLMLGNAVYTFTISAFGKNGWSYWDGKQS
                     SSPYYYGLLKFIDMVKTSKPIIEYDKILEVIAVLKAIEISYQSGAEVFLEDLI"
     gene            833..1579
                     /locus_tag="P5310_07910"
     CDS             833..1579
                     /locus_tag="P5310_07910"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRU33318.1"
                     /translation="MREYFDFNVMIGAPTVVQLGKWLDTRMLSEEMRRFNIKKAIAYH
                     SYSRDYDAYFGNNYMIKEITGYSDILPCWVLLPFDISPTNTPDELISQLKENNVKIVR
                     LFPELHNFLLEEEVVGDLLALLEGYNVPVLISPNKVGWRDIYSILRNHPNLRLILVDL
                     NYRNSSYIFPLLKRYKNVYIELSGYTTYLGIEGIYKRFGGEHMLFWSNLHYKSPGITI
                     FYVENADVPEEIKDLIAYKNGERLIEEVTL"
BASE COUNT          575 a          273 c          379 g          500 t
ORIGIN      
        1 cctgaaggga ttaatggttt aaaagtagaa gatgccagtg tggtagctat ctggggtttt
       61 gaaagaaaaa ggacagagga ggtagcaagt gccggcaaga tccccgagat agtaagttat
      121 cctgaagata tgataggaaa agtggatgct gtaatggttg tattaaggga tggagcattc
      181 cactataaat tttccaagcc atttattgag gcagggattc caacctttat agacaaaccg
      241 attgaaacat caatagacaa tgcaaaaagg attatagaac ttgcaaatga gaaaaagact
      301 cctatcacct ctttctcaac cctcagatac gatgaaccga tagtaaaatt taaagaggac
      361 ctatcctcta aaggaccttt aactaccgga tttgtatgtg gtcctgccga tgctaccagt
      421 gaatatcagg ggatattctt ctatggagtt cacatagtgg agttgtgtca agaaatattt
      481 ggaagtggaa tagagagggt gatggctgta aaccatcaca ataacatagc gattaccctt
      541 acctattctg acgaaaagat tattgagctc ttaatgctgg gaaatgccgt ctatacattc
      601 accatatcag catttggtaa gaatggatgg agttattggg atgggaaaca gtcctcctct
      661 ccatattatt atgggctttt aaagtttata gatatggtaa agacatcaaa acctatcata
      721 gaatatgaca aaatcctgga agtgatagct gttctaaagg cgatagaaat ctcttatcaa
      781 agcggtgcag aggtattctt agaggattta atataaagag aaggaagaaa agatgagaga
      841 atacttcgat tttaacgtaa tgataggagc gcccaccgtt gtacaattgg gaaagtggct
      901 ggatactaga atgctttctg aggagatgcg cagatttaat attaagaaag ctatagccta
      961 tcatagctat agcagggact atgatgctta ttttggcaat aattatatga taaaagaaat
     1021 tacagggtat tctgatattt taccctgctg ggttcttctt ccatttgaca tatctccaac
     1081 aaatactcca gatgaactta tatctcaatt gaaagagaat aatgttaaaa tagtaaggct
     1141 tttccctgag cttcacaact ttctcttaga agaagaggtt gtaggagacc tgttggcttt
     1201 actggaggga tataatgtcc ctgttcttat ctcacccaac aaggtcggat ggagagatat
     1261 ttacagtatc ttgaggaatc atccaaatct cagacttatt ctggtggatt taaattacag
     1321 gaatagttcc tatatattcc cccttttgaa gagatataaa aatgtataca tagaactctc
     1381 aggttatact acttatcttg gaatagaggg tatatataaa aggtttggag gagaacatat
     1441 gctcttttgg agtaatcttc actataaaag tccgggcata accatattct atgtagagaa
     1501 tgcagatgtt ccagaggaga ttaaagacct catagcttat aaaaacggtg aaaggctcat
     1561 cgaggaggta actctatgat cgatgcacat gcccatcttg gaaaatggtt taacttctat
     1621 tacgatggtg gagattatct ctctatggtc agaattatgg ataaaatcgg tatgaagagc
     1681 gcctggataa gtgcattgat aggtatagga cctaattata aactggg
//
LOCUS       DAOTKU010000378         1723 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_56241_length_1723_cov_3.786571, whole genome shotgun sequence.
ACCESSION   DAOTKU010000378 DAOTKU010000000
VERSION     DAOTKU010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56241_length_1723_cov_3.786571"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="P5310_07915"
     CDS             <1..252
                     /locus_tag="P5310_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HRU33319.1"
                     /translation="DPKYREKWLDPDVHPKEYKPAVIDYLLKYGRRAPSYYLRNWGKI
                     SDIMYQELSNVWLGKISAKTACVSIATKIKPLLTGRYDR"
     gene            311..1210
                     /locus_tag="P5310_07920"
     CDS             311..1210
                     /locus_tag="P5310_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007431939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRU33320.1"
                     /translation="MTKSKSLSNEAKWGIVLALPAILGFLIWTLGPMVASFILSFTDW
                     NVVSTPHFIGIENFRKMFMEDFIFKKSIYATLYYTLGSVLSTVIFAFLIALLLNQKIK
                     GLPIFRTIFYLPSITPAIASAMLWIWLFNPDFGLLNQLLGRFGIPRLQWIYDEKQVIP
                     SLIFMSIWGMGGTMIIFLAGLQGIPQEFYEAVDMDGGNWWHKFRYITIPMMTPTIFFN
                     LVMAIIGTFQVFTSAYIMTSGGPNYASMFYVYYIYLSAFRYGDMGYACALAVILFFII
                     LSFTLVVFRSSPYWVYYESARRR"
     gene            1221..>1723
                     /locus_tag="P5310_07925"
     CDS             1221..>1723
                     /locus_tag="P5310_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016821430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRU33321.1"
                     /translation="MKIGKTASTRINRLIVYIALILGSIIFILPFVWLVRSSFMELGQ
                     IFILPPEWIPKPFRWQNYPEALKAAPFFTYFLNTLKILIFNEAGVILTAITCAYSFSR
                     LRWPGRDLVFAVLLTSMMLPYAVTLIPTFILWKILGGLNTFYPLTVPAWFGGGMFYVF
                     LLRQFFFS"
BASE COUNT          483 a          297 c          353 g          590 t
ORIGIN      
        1 gatcccaagt acagggagaa gtggttagat ccagatgttc atcctaagga gtataaacca
       61 gcagttatag actatctgct gaagtatgga agaagagctc caagttatta tctgagaaat
      121 tggggtaaaa tatcagatat tatgtaccag gagttgtcta atgtatggtt agggaagatt
      181 tctgctaaaa cagcttgcgt tagtatagct acaaagataa agccattatt aacaggaagg
      241 tacgataggt agatgaattt aaaaggacca gggtgtaagc cctggtcctt gaatgtacag
      301 ggtgatatag atgactaaaa gcaagtctct tagtaatgaa gctaaatggg gaatagtttt
      361 ggctttacct gccatattgg gattccttat ctggacatta ggtccaatgg tagcaagctt
      421 tatcttaagt ttcactgatt ggaatgttgt atccaccccc cattttattg ggatagaaaa
      481 ttttagaaag atgtttatgg aagactttat ttttaaaaag tcgatatatg ctactctata
      541 ttataccttg ggcagtgtct tatccacggt aatatttgct tttttgatag cccttctatt
      601 aaaccaaaag ataaagggtt taccaatatt tagaactata ttttatcttc cctcaattac
      661 ccctgccatt gccagcgcaa tgctctggat atggctattt aatccagatt ttggattact
      721 taaccagttg ctgggaagat ttggtatccc cagacttcag tggatatatg atgaaaagca
      781 ggttatacca tcccttatct ttatgagcat atggggtatg gggggaacga tgattatttt
      841 tttggctggt ctacaaggta ttcctcagga attctatgag gctgtggata tggatggagg
      901 aaattggtgg cataaattta gatatattac tatccctatg atgactccaa caatattctt
      961 taaccttgtt atggctatta taggcacctt tcaggtcttt accagtgcct atataatgac
     1021 cagtggtggt cctaactatg caagtatgtt ctatgtatac tatatttacc tctctgcctt
     1081 tagatatgga gatatgggat atgcctgtgc acttgcagtg atactcttct ttataatact
     1141 ttcatttaca ctcgtcgtct ttagatcttc tccgtactgg gtttactatg aaagtgctag
     1201 aaggaggtaa atgcctttaa atgaaaattg ggaaaactgc ttcaacaaga attaacagat
     1261 tgattgtcta tatagccctt atcttagggt ccatcatctt tatactgcca ttcgtatggt
     1321 tggttagaag ttcttttatg gaattaggac agatctttat acttcctcca gaatggattc
     1381 caaaaccgtt cagatggcag aattatcctg aggctcttaa ggcagctcct tttttcacct
     1441 atttcctgaa taccctcaag atacttatct ttaatgaggc aggtgtcata ttaactgcta
     1501 tcacctgtgc atatagcttt tcccgtttaa ggtggccagg aagagattta gtcttcgctg
     1561 tattattaac atccatgatg ctcccgtatg cggttacact gattccaaca tttatactct
     1621 ggaagatatt gggtggatta aatacctttt atccattaac tgttcctgcc tggtttggag
     1681 gaggaatgtt ttatgtgttt cttctgaggc agttcttttt ttc
//
LOCUS       DAOTKU010000379         1717 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_56537_length_1717_cov_2.403129, whole genome shotgun sequence.
ACCESSION   DAOTKU010000379 DAOTKU010000000
VERSION     DAOTKU010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56537_length_1717_cov_2.403129"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1226)
                     /locus_tag="P5310_07930"
     CDS             complement(<1..1226)
                     /locus_tag="P5310_07930"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRU33322.1"
                     /translation="MKFLKCLIVISVCMGLVLGVVPISFSQEKSSLGQYPSISEYQKA
                     TGKKITKFNEAPALDDLVKQGKIPSVEKRLPDEPAVVEPEEEIGQYGGTWRRAALSPS
                     DTMIHMRLGYEPMVKWARDGKTVIPNLCTSWKVGEGGRAYTFYLRKGLKWSDGEPFTA
                     DDVMYWYNDELLNKEVSPVFPSWLAPAGEPVKVEKVDNYTVRFRFKRPYSLFLEYIAV
                     NNAILASPKHYLQQFHIKYTPKEKLDAMVKEAKRDFWYQLYGDKASAYSNPDLPVLRP
                     WKLATSPTGPRATAERNPYYWKVDTAGNQLPYIDRVAFDIDRIAWDMVQNTEMITMKA
                     VQGEIDMQGRNLAFKDFTLLMENREKGGYQVYQWDAGETGSAVFFNQNYTKDKFIAGL
                     LKDVRFRTALSLAINR"
     gene            complement(1407..>1717)
                     /locus_tag="P5310_07935"
     CDS             complement(1407..>1717)
                     /locus_tag="P5310_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HRU33323.1"
                     /translation="PKVAFINFITDVSPDCDCSGWHDASIVPDIGILGSHDIVAIDQA
                     SVDLVNKSIGLKESALSKSFNPGEDKLRDIHPHIDWNVQLSYGEELGLGSREYTLKKI
                     "
BASE COUNT          443 a          407 c          293 g          574 t
ORIGIN      
        1 tctctattta ttgccagaga taacgcagtt ctgaatctta catcttttag tagacctgct
       61 atgaatttgt cctttgtata gttctggtta aagaataccg cgcttcccgt ttctcccgca
      121 tcccactgat atacctgata accacctttt tctctattct ccataagaag ggtaaaatct
      181 ttaaatgcaa ggtttctccc ctgcatatct atttcgccct gcaccgcctt cattgtaatc
      241 atctctgtat tctgtaccat atcccaggca atcctgtcta tatcaaatgc tactctatct
      301 atatatggta gttgatttcc agcagtgtcc accttccagt agtaaggatt tctctcggct
      361 gtagctcttg gtcctgtagg agatgtagct aatttccacg gtctcagtac agggaggtca
      421 gggttagaat atgcagatgc cttatccccg tacagctgat accagaaatc tctctttgcc
      481 tctttaacca tggcatcaag cttttcctta ggggtatatt ttatgtggaa ctgctgcaga
      541 taatgctttg gagatgccag tatggcattg ttaaccgcta tatactccag gaatagagaa
      601 tatggtctct taaatctaaa tcttaccgta tagttatcta ctttttctac ctttacaggt
      661 tcaccagcag gagccagcca ggatggaaat actggtgaga cctctttgtt cagcagttca
      721 tcgttatacc agtacattac gtcatcagcg gtgaatggtt caccatcgga ccatttgagt
      781 cctttcctca ggtagaaggt gtatgctctt cctccctctc ctaccttcca ggatgtgcat
      841 agatttggga ttacagtctt tccatctctt gcccatttaa ccataggctc ataaccaagt
      901 ctcatatgta tcatagtatc ggatggtgat agtgctgctc tcctccaggt tccaccgtac
      961 tgcccgatct cttcttcagg ttccaccact gccggttcgt ctggaagcct tttttctacc
     1021 gatggaattt tcccctgctt tacaagatca tctaatgctg gtgcttcatt aaactttgtt
     1081 atctttttcc ctgttgcctt ttgatactca gagatgctgg ggtactgtcc cagactggat
     1141 ttttcctgag aaaaggagat cgggacaact cctaagacta agcccataca cactgatatt
     1201 actataagac actttaaaaa cttcatagaa ttacctcctc gtatattttt ttaaaacaaa
     1261 ttagacctaa ctccagatct tatgttgaac tcttttatac cctcatacac atcattccct
     1321 tctttagata tattaataat taagttatat gaaaccgata aaagtatatc ataaacctga
     1381 gcaatataca agtttgttaa ataatactat atctttttaa gtgtgtattc cctggaacct
     1441 agccccagct cctcaccata tgaaagttga acgttccagt ctatatgagg atgtatatct
     1501 ctcagtttgt cttctcccgg attaaaactc tttgaaagag cagattcttt taatcctata
     1561 ctcttattaa ccaggtctac tgaagcctga tctattgcta ctatgtcgtg acttcccagg
     1621 atacctatat ccggcactat actggcatcg tgccatcctg agcagtcaca atcgggactg
     1681 acgtccgtaa taaaatttat aaatgcaacc ttaggtt
//
LOCUS       DAOTKU010000380         1711 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_56802_length_1711_cov_3.101449, whole genome shotgun sequence.
ACCESSION   DAOTKU010000380 DAOTKU010000000
VERSION     DAOTKU010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56802_length_1711_cov_3.101449"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="P5310_07940"
     CDS             <1..458
                     /locus_tag="P5310_07940"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protease modulator HflK"
                     /protein_id="HRU33324.1"
                     /translation="IQNRTADLIQSVLDRYKCGIRVVAVQLQDVDPPEAVKAAFDEVQ
                     KAKEKMDQLVNEGRRYYNQVVLEAEGEAYKLIKEAEAYKVQKVNLAKGEVDRFDEVYK
                     QYVKGKDIARKQIYMDRMRELLTGINKYIVDPSVGLNIFLQKGDVEVIK"
     gene            455..1321
                     /locus_tag="P5310_07945"
     CDS             455..1321
                     /locus_tag="P5310_07945"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HRU33325.1"
                     /translation="MRILSRIEFIVFIVLLALIIVRGLFLYQLDQTEQAVITRFGKII
                     RVDKTPGLGFKLPLIDIVNRFSKKILEYDSKSQVAVTKDKKTIIVDYYAKYRIESPDK
                     FLQRVRTQQGANTILDDVVYSSMRKDVGKYTLSEIITSREEIPNLVIKDLKILLPEYG
                     LDIVDVRFKTTNFPEEVQASVFERMKAERLQMAQKYISEGEKQKISIMADADKVKAEL
                     ISKANMESQKIRGEGDRTAVSIIQDAYAKAPELAFMMKYMDTYRDIIKKDDTLVILST
                     ESEIFKILQDME"
     gene            1339..>1711
                     /locus_tag="P5310_07950"
     CDS             1339..>1711
                     /locus_tag="P5310_07950"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HRU33326.1"
                     /translation="MKLFIEYSHNLKPHHLEMILSSRDDITLVDKIESAEVVLGGVSR
                     DEFKRAKNLKWIQTTGAGVDSLLYPEIIESDVIITNASGVHPIPIAEHTFALILAITR
                     GLIKSFEGKTRKTWFHNEAYTD"
BASE COUNT          616 a          262 c          412 g          421 t
ORIGIN      
        1 atatccagaa tcgtactgca gaccttatcc aatctgtact tgacagatac aaatgcggta
       61 taagggtggt ggctgttcaa ttacaggatg tggatccgcc cgaagcagta aaagctgcct
      121 tcgacgaagt ccagaaggct aaggaaaaga tggaccagct ggtaaatgag ggaaggagat
      181 attacaatca ggtggtgctg gaagctgaag gagaagctta taaacttata aaggaagcgg
      241 aggcttataa ggttcaaaag gtaaacctgg ctaagggaga agtggataga ttcgatgagg
      301 tttacaagca gtatgtgaag ggaaaggaca tagccaggaa gcagatctac atggatagaa
      361 tgagggaatt actcacagga ataaataagt acatagtcga cccttcagta ggattgaata
      421 tctttcttca gaagggggat gtggaggtaa taaaatgaga atccttagca ggatagaatt
      481 catagttttt attgttcttc tggcacttat aatagtgagg ggattatttc tatatcaact
      541 agatcagaca gaacaggctg ttatcacaag attcggtaag ataataagag tagataaaac
      601 ccctggactt ggcttcaagc ttcctctcat agatattgta aacagattca gcaagaagat
      661 tctggaatat gatagcaaat cacaggtggc agtgactaag gataaaaaga ctataatagt
      721 agattactat gccaagtaca ggatagaatc tcctgataaa tttttacaga gggtaaggac
      781 acagcaagga gcgaatacca tacttgatga tgtggtatat tcttctatga gaaaagatgt
      841 gggaaaatat actttatctg aaattattac aagcagagaa gagataccta atctagtgat
      901 aaaagactta aaaatactgc tccctgaata tgggctagat atcgtagatg taagatttaa
      961 aaccaccaac tttcctgaag aagttcaagc atctgtattc gagaggatga aggctgaaag
     1021 gctgcagatg gctcaaaaat atatttcaga aggagaaaag cagaagatat ccattatggc
     1081 tgatgcagac aaggtgaagg cagaacttat ttctaaggct aatatggaga gccagaagat
     1141 acggggagaa ggcgacagga cagcggtctc tataattcag gacgcctatg caaaagcgcc
     1201 agaactggca tttatgatga aatatatgga tacatatagg gatataataa aaaaagatga
     1261 tacactggtt atactttcca cagaatctga gatatttaaa atacttcaag atatggagtg
     1321 agataaggag gtaaaggttt gaagctattt atagaataca gtcataatct caaacctcat
     1381 catctggaga tgattctctc atcaagagat gatataactt tggttgataa gatagaatca
     1441 gcagaggtgg tacttggggg tgtatccagg gatgagttta agagggcaaa aaacctgaag
     1501 tggatacaga ctaccggggc aggggtggac agtctgctct atccagagat aatagaaagc
     1561 gatgtaatta taaccaatgc cagcggcgtt catccgatac caatagcaga acatacattt
     1621 gcccttatat tagccattac cagagggtta ataaaatcat ttgagggaaa aaccagaaag
     1681 acctggttcc ataatgaggc atacacagac g
//
LOCUS       DAOTKU010000381         1710 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_56866_length_1710_cov_2.400604, whole genome shotgun sequence.
ACCESSION   DAOTKU010000381 DAOTKU010000000
VERSION     DAOTKU010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56866_length_1710_cov_2.400604"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>309
                     /locus_tag="P5310_07955"
     CDS             <1..>309
                     /locus_tag="P5310_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRU33327.1"
                     /translation="LKLRKIPRREIEQKVQEVAEMLGIQELLKRKPKELSGGQRQRVA
                     LGRAIVRTPQVFLMDEPLSNLDAKLRVQTRAELKRLHNRLQVTTIYVTHDQVEAMTMG
                     D"
     assembly_gap    310..319
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <320..809
                     /locus_tag="P5310_07960"
     CDS             <320..809
                     /locus_tag="P5310_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HRU33328.1"
                     /translation="MNNGVIQQIDSPLNLFNKPRNLFVAGFIGSPAMNFLNTVLEKKN
                     GGYWLNGGSFEVKIPESLNSLVEPYATKEVVFGIRPQDIYDAELMREAEIKPEWTIKV
                     TVDVTEPLGSEVNLYLTTGEHVIVATVDPATKAQTGLEWRVFFDMDKMHLFDPNTGEA
                     IV"
     gene            941..1438
                     /locus_tag="P5310_07965"
     CDS             941..1438
                     /locus_tag="P5310_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRU33329.1"
                     /translation="MDTIIAIRSRRSVRKYLNKEISKEILEDIVDCGRLAPSGYNEQP
                     WIFVVVVDREMKEKVAEATTSGKFIRDAGACIAVFCKKTAETALEDASAATENMIISA
                     QAYGLGTCWINVYKKSHSPKIKEILNCPEDMELMTLISIGYPAEEPKTPPKKSLNEVL
                     RWNKF"
     gene            complement(1474..>1710)
                     /locus_tag="P5310_07970"
     CDS             complement(1474..>1710)
                     /locus_tag="P5310_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011916429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HRU33330.1"
                     /translation="EDINSPRQSFICEKLKLEEFRNLIDSTWALIGHEMLATEDISKG
                     MFSEESKRKCPDLNERIRVYSEIGRKSSVSREQG"
BASE COUNT          538 a          281 c          401 g          480 t
ORIGIN      
        1 ctgaagttga ggaagattcc aagaagggag attgaacaaa aagttcaaga agtggctgag
       61 atgcttggta ttcaggagct tctgaaacgt aagccgaagg aactgtcagg tggacagagg
      121 cagagagtag ccctgggtag agctatagta aggacaccac aggtattcct tatggatgaa
      181 cctttgtcaa accttgatgc caagttaagg gttcagacaa gggcagagtt gaagagattg
      241 cacaacaggc ttcaggttac aacgatctat gtgacccatg accaggtaga ggctatgact
      301 atgggtgatn nnnnnnnnna atgaataatg gggtgatcca gcagatagat tctccgttga
      361 atctgttcaa taagccaaga aatctctttg ttgccggttt tattggtagt ccagcgatga
      421 actttttaaa tactgtgctg gaaaagaaga atggtggtta ctggttaaac ggtggaagct
      481 ttgaagtaaa gatacctgaa tctcttaact ctcttgtgga accgtatgca acaaaagagg
      541 ttgtctttgg tataagaccc caggatatct atgatgcaga gcttatgaga gaagcggaga
      601 taaaacctga gtggaccata aaggttaccg ttgatgtaac tgaaccattg ggttcagaag
      661 ttaacctgta tctgacaaca ggagaacatg ttatagttgc tacagtagac ccagccacaa
      721 aggcacagac aggattagag tggcgagtat tctttgatat ggataagatg catctttttg
      781 atccgaatac aggggaagct attgtataaa agttttaact ttagagagtt agaaattatg
      841 aggtgacact ccatagaggg tgtaccctaa gtataaaagg gaaggatttt tttaccttcc
      901 cttttctttt tttatcttct gctataagaa ggagggattt atggatacaa taatagctat
      961 aaggtctaga agaagcgtaa gaaagtacct gaataaagag atctccaaag agatattgga
     1021 agatatagtt gattgtggaa gattagctcc atctggttat aatgaacaac cctggatatt
     1081 tgtggttgtg gttgatagag agatgaaaga gaaggttgca gaagccacca catccggaaa
     1141 gtttataaga gatgctggag cctgtatcgc agtattctgc aagaagacgg ctgaaacagc
     1201 cttagaagat gcctctgcgg caactgaaaa tatgattata tcggcgcagg cttatggatt
     1261 aggaacgtgc tggataaatg tctataaaaa gtctcattcc ccaaagataa aagaaatatt
     1321 aaactgccct gaggatatgg agcttatgac tcttatatcg attgggtatc cggcagaaga
     1381 acccaaaact cctccaaaga aaagtttgaa cgaagtattg agatggaata aattttagag
     1441 gtactgattt taaggttaag agagaggtag agattaacct tgttccctac taacgctact
     1501 ttttcttcct atctctgagt atacccttat cctttcatta aggtctggac attttctttt
     1561 gctctcttct gaaaacatac ccttacttat atcttcggta gccagcatct catgtcctat
     1621 aagagcccag gtagaatcta taagatttct gaattcttcc agtttcagct tttcgcagat
     1681 aaatgattgt ctgggagaat ttatatcctc
//
LOCUS       DAOTKU010000382         1702 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_57236_length_1702_cov_2.134791, whole genome shotgun sequence.
ACCESSION   DAOTKU010000382 DAOTKU010000000
VERSION     DAOTKU010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57236_length_1702_cov_2.134791"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1200)
                     /locus_tag="P5310_07975"
     CDS             complement(<1..1200)
                     /locus_tag="P5310_07975"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRU33331.1"
                     /translation="MRRVILVLIVLGLLFSVLGGSSLAKFVQSPGVSVALYATPEEYQ
                     QSTGKRITKFSEAPDLAELVKQGKLPSVDKRLPKDYMVYKGENIGQYGGDLKSASVGP
                     AGGHELYCMTDPPPLFTTDAYARSIIPYVAKGYEFSKDMKKLKIYLREGLRWSDGNPF
                     TADDIMFWWNDIILNNELTPVKPKIWMPGGKLARFTKVNDYTLEISFLVSYRPMMTSP
                     TVIQAIGYTPAHYVKKWHKKYNPDADKIAKQEGYDTWYKCFSFHNDAGWTQQDTNRPG
                     LGSWVMERITTTQKVYRRNPYFFGVDGEGNQLPYIDRIIVDIVANNQVVTMKLLSGES
                     SIGGYMAGMMMKDFTLYKENESKGNYRVSLYQSIYDEVVFSFNQAHPDPVIRKILQDV
                     RFRQAMSL"
     gene            complement(1301..>1702)
                     /locus_tag="P5310_07980"
     CDS             complement(1301..>1702)
                     /locus_tag="P5310_07980"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRU33332.1"
                     /translation="ILKRLVEEELNSFTLIVWRHYSSSFSDLISNTKDFISAHLSETI
                     TLKLMAEKLGVNPSYLSRRFKQKEGIILTTYINMKRIEEAKRLLISRNSTITDVAFDV
                     GFGSIQQFNRVFKKIEGCSPSRWRANIVKNK"
BASE COUNT          499 a          376 c          271 g          556 t
ORIGIN      
        1 cagagacata gcctgtctga atcttacatc ctgtaagatc tttcttatta ctggatctgg
       61 atgtgcctga ttaaaagaaa ataccacctc atcatatata ctctgatata gagatacacg
      121 ataatttccc ttactctcat tctctttata aagggtaaag tccttcatca tcataccagc
      181 catataaccg ccaatacttg actctccact tagaagtttc atggtaacta cctgattatt
      241 tgccactata tctacgatta tcctatctat atatgggagc tggttcccct caccatctac
      301 cccaaagaaa tatgggttac gtctatagac cttttgagtt gttgtaattc tttccataac
      361 ccaagacccc agtcctggac ggttagtatc ttgctgagtc catcctgcat cattgtgaaa
      421 agaaaaacac ttataccatg tatcataacc ttcctgtttt gctatcttat ctgcgtcagg
      481 attatatttt ttgtgccact tctttacata atgagctggt gtgtatccta tagcctgtat
      541 tacagtagga gaagtcatca ttggtctata agaaaccagg aatgaaatct caagggtgta
      601 atcgttgact tttgtaaacc tggcaagctt accaccaggc atccatatct taggctttac
      661 gggagtaagt tcattattta ggataatatc attccaccag aacattatgt catcagcggt
      721 aaaaggattt ccatctgacc atcttaagcc ttccctgagg tatattttaa gcttcttcat
      781 atccttagag aattcatatc cttttgcaac atatggaatg atgcttcttg catatgcatc
      841 tgtggtgaag agaggcggtg gatcggtcat acagtatagt tcatgaccac cagctggtcc
      901 cacagaagcg cttttcagat ctccaccata ctgaccgata ttctctccct tatagaccat
      961 ataatccttg gggagtcttt tatctacaga tggaagcttc ccctgtttca ccagctctgc
     1021 taaatctgga gcctcgctaa attttgttat ccttttacct gtactctgct gatactcttc
     1081 tggggtggcg tataaggcta cggatacccc gggagattga acaaatttgg caagagacga
     1141 acctcctaaa acagaaaaca acaagcccag aacgattaaa actaaaatga ccctcctcat
     1201 taatctcaat cctcctaact ttaaattttt ggaacatact tccgataact acatgcttct
     1261 aaaacataac agtaaacggc aaggataaca acacaaatat ttacttattt ttaactatat
     1321 ttgccctcca tctagaggga ctgcaccctt cgatcttttt aaatacacga ttaaactgtt
     1381 ggatacttcc aaacccaacg tcaaaagcca catcagttat agttgagttc ctgcttataa
     1441 gtaatctttt agcttcttct attctcttca tatttatgta agtagttaaa ataatacctt
     1501 ctttttgttt aaatctcctg ctcagataag aaggatttac ccctagtttt tctgccataa
     1561 gctttaaggt tatagtctca gacagatgag cgcttataaa atcttttgta ttacttataa
     1621 ggtctgaaaa tgaggatgaa tagtgtctcc aaactataag agtaaagcta ttcagttctt
     1681 cttctactaa cctcttaagt at
//
LOCUS       DAOTKU010000383         1695 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_57517_length_1695_cov_2.578049, whole genome shotgun sequence.
ACCESSION   DAOTKU010000383 DAOTKU010000000
VERSION     DAOTKU010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57517_length_1695_cov_2.578049"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="P5310_07985"
     CDS             <1..406
                     /locus_tag="P5310_07985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HRU33333.1"
                     /translation="EYAILQDIQYHPYKLDVLHVDWHGINLEEEIETTIPIIIKGEAA
                     GIKVGGILEVVLEEIGIRCLPINLPPHIEVDISNLNIGQSIHVRDLKLPEGVTLVTHE
                     DEVIVTVVAPKVGEEAPAAAEEVTQPQEEVSE"
     gene            471..1040
                     /gene="pth"
                     /locus_tag="P5310_07990"
     CDS             471..1040
                     /gene="pth"
                     /locus_tag="P5310_07990"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HRU33334.1"
                     /translation="MIRLIVGLGNPGLRYSNTRHNAGFMFVDTAVSRWGEWVEETGRG
                     ILGKISLDGNDTLILKPLTYMNRSGYALPPLVERFSLSPQEILVVYDDLWIPLGSVRI
                     RIKGSSGGHNGMQSIIDTLETEDIPRLRIGIGPLPENASSVDFVLSDFSEEELKILEK
                     TIDSILEGLPTIILDIDKAMSRYNKRVIE"
     gene            1037..>1695
                     /locus_tag="P5310_07995"
     CDS             1037..>1695
                     /locus_tag="P5310_07995"
                     /inference="COORDINATES: protein motif:HMM:NF037626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU33335.1"
                     /translation="MSSVFSNLSRANSLVRLVEDVKKNKSSTVGINLSSLIPVITGYL
                     SLNLKVPIIVVVEDEENVVRIANDINALFDIDTTYLIYDPTDRENRWRNASLAGGFVR
                     GEFSVLVATGSGIKDFPAVGLSLTITKESKWDFIIDRLTSLGYRRVFMVERRGEFSIR
                     GGIVDIYPVNLTHPVRLDFFGDEIASIRKFHTLTQKSVGEMDTPVNVLPVNPAEVSSN
                     V"
BASE COUNT          527 a          272 c          418 g          478 t
ORIGIN      
        1 agagtatgcg atactccagg atatacaata ccatccatat aagcttgatg tgttgcatgt
       61 agattggcac gggataaacc tggaagaaga gatagagaca actataccaa ttattataaa
      121 gggtgaggcg gcaggaataa aggtgggagg aatacttgag gtggtattag aagagattgg
      181 tataagatgt ctcccaatta accttcctcc acatatcgag gtggatataa gtaatcttaa
      241 tataggtcag agtatacatg taagagacct gaaattgcca gagggggtaa cccttgttac
      301 ccatgaagat gaggtgatag taacggtggt agctcctaaa gtaggtgaag aagctccagc
      361 ggcagcagaa gaagttaccc agcctcaaga agaggtttct gaatagcttt tgtaccgggt
      421 ggctggtaaa taatattacc tgtcgcccgg taatttataa gcttcatatt atgataagac
      481 ttatagtcgg tttaggaaat cctggcttaa gatattctaa tacccgtcat aatgcaggct
      541 ttatgtttgt agatacagcg gtatccaggt ggggagaatg ggttgaggag acaggtagag
      601 gtattctggg taagatatct ctggatggta atgacaccct gattctaaaa cccctcacat
      661 atatgaacag gagtggttat gccctcccgc cgttagtaga gagattttct ctatcacctc
      721 aggagatact cgtagtttac gatgacctgt ggatacctct tgggagtgtt agaataagaa
      781 taaagggctc cagtggtgga cataatggta tgcagtcaat aatagataca cttgaaacag
      841 aagatatacc aagattgagg attggcatag gacctttacc tgaaaatgca agcagtgtag
      901 attttgtcct ttctgatttt tctgaggagg aattgaagat actggaaaaa actatagatt
      961 ccatccttga aggtttgcca accataatcc tggatataga taaggcgatg agcaggtata
     1021 ataaaagagt tatagaatga gttctgtttt ttcaaatcta tctagggcaa attctctagt
     1081 tagactggta gaagatgtta aaaagaataa atcttctact gtagggataa atttatcctc
     1141 tctgatacca gtaattaccg gttatctttc tttgaacctg aaagttccca taatagtcgt
     1201 tgtggaagac gaagagaatg tggtaaggat agcgaatgat ataaatgcac tgttcgatat
     1261 agatacaaca tatcttatct atgacccgac cgatagagag aataggtggc ggaatgccag
     1321 tctggcgggc ggttttgtaa gaggtgaatt ttctgtcctg gtagctactg gcagtgggat
     1381 aaaggatttt ccagctgtag gtctctcact tacaataacg aaggaaagta agtgggattt
     1441 tattatagat agactcacct ctcttggcta caggagagtt tttatggtgg agagacgggg
     1501 agagttctcc ataagaggtg gaatagtaga tatctatccg gtaaatctaa cccatcctgt
     1561 aagattggat ttctttggtg acgagatagc aagtatcaga aaatttcaca ctcttaccca
     1621 gaagtctgtt ggagagatgg atacaccagt caatgttttg cctgtaaatc cggcggaggt
     1681 cagttcaaat gttga
//
LOCUS       DAOTKU010000384         1687 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_57923_length_1687_cov_3.058824, whole genome shotgun sequence.
ACCESSION   DAOTKU010000384 DAOTKU010000000
VERSION     DAOTKU010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57923_length_1687_cov_3.058824"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..1152)
                     /gene="murG"
                     /locus_tag="P5310_08000"
     CDS             complement(79..1152)
                     /gene="murG"
                     /locus_tag="P5310_08000"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HRU33336.1"
                     /translation="MKKILILAGGSGGHLYPALAVADILRENGFKVYIFTSKKRPSGI
                     DFNGLEVIEGYSVGWDRSILGGFKTIWSLGKDFIFSFKILRGIKPDLILGMGGYISIS
                     PIFASLMFNIPRVLHEQNIISGLANRILALFVDRIFVSFPDTEFGISQSKVVYTGLPL
                     RRELYQVKPSGNKDRFTILVMGGSQGARIINDTVLEIVEKGLLDGIKIIHITGPKEFE
                     RLRDRIEKVNYPHYKVYGFVKDIWNLYREADLVVSRAGAGSVIEFATVSIPAILIPYK
                     GAEGHQYLNARWLADSGGAIIIEQDQLSALSLVEAILNIVKSDKINEMRESISRLNLP
                     SGSYILTEELIKIITGGSHDKNK"
     gene            complement(1156..>1687)
                     /locus_tag="P5310_08005"
     CDS             complement(1156..>1687)
                     /locus_tag="P5310_08005"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HRU33337.1"
                     /translation="ASSYLGYKLIFEASYREERFLAFLDPWRDPLGSGYQYIQALKSF
                     SAGGLFGAGLGRGRQKLNILPEVQTDLILAHIGEELGFIMTIAIIFLYALICLNMFKI
                     SRDCPIKFSRIVVLGFAISFSIQAIINMGGELKLLPLTGIPLPLLSSGGTSRIVSLFM
                     IGYSLGASRYRLEETG"
BASE COUNT          522 a          396 c          241 g          528 t
ORIGIN      
        1 tctgtcatag ggtctttagg atcagagtat ttactcatac cataaccacc aatacctata
       61 aaatggaacc tgtcttccct acttattttt atcatggctt cctcctgtta taatctttat
      121 aagctcttcc gtcagtatat agctaccact gggtaaattc aatctggaga ttgactctct
      181 catttcatta atcttatcag attttacaat gtttagaatc gcttctacaa gacttaacgc
      241 tgacaattga tcctgttcta taataatagc cccaccagaa tctgccagcc acctggcatt
      301 gagatattga tgcccttcag ctcctttata tgggatcagt attgcaggta tagagactgt
      361 ggcgaattct ataactgaac ctgccccagc tctggacacc actaaatctg cctctctgta
      421 tagattccat atatccttta caaatccgta aactttataa tgtggataat taactttctc
      481 aattctatct cttaatcttt caaactcttt aggacctgtg atatgtataa tctttatacc
      541 atctaacaat cctttctcca caatttccag aactgtatcg ttaattattc ttgccccctg
      601 acttccaccc ataaccagta tggtaaatct atctttattg ccagaaggtt ttacctgata
      661 taattctctt cttaaaggta acccagtata taccaccttc gactgagaga taccaaattc
      721 agtatcggga aaggagacaa atatcctgtc cacaaatagg gctaggattc tattggctaa
      781 acctgatatt atattctgtt catgcaatac ccttggaata ttaaacatta aagaggcaaa
      841 gataggagat attgatatat accctcccat acctaatatt aaatctggct taattcctct
      901 cagaatttta aaagaaaaaa taaaatcttt tcctaaactc cagatggtct taaaccctcc
      961 caatatactt ctatcccagc ctaccgaata gccctcaatg acctccagtc cattaaaatc
     1021 aatcccagaa ggtcttttct tgcttgtaaa tatgtacacc ttaaatccat tctctctgag
     1081 gatatctgct acagctaaag ctggatatag atgtccacca ctaccacccg ccaatatcag
     1141 tatttttttc attatctagc ctgtctcctc caatctgtat ctggaagccc caagggagta
     1201 accaatcata aatagagaaa ctattctgct tgtcccacca gagctcagca atgggagcgg
     1261 tatacctgtc aatggtaaca gctttaattc gccacccata ttaataattg cctgaatgga
     1321 aaagctgatg gcaaaaccaa gaaccactat cctagaaaat tttatgggac aatctctact
     1381 tattttaaac atattcagac agatcagcgc atagagaaaa ataatcgcga tggtcattat
     1441 aaaacctaat tcttccccta tatgggctaa tataaggtct gtttgaacct ctggcaatat
     1501 attgagcttc tgtcttcctc ttcctaatcc tgctccaaat aatcctccag cagaaaaaga
     1561 ttttaatgcc tgtatatact gatacccgct ccccaaaggg tctctccatg ggtcaagaaa
     1621 tgccaggaac ctctcttctc tataagaagc ttcaaatata agtttataac caagataaga
     1681 agatgct
//
LOCUS       DAOTKU010000385         1687 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_57942_length_1687_cov_1.998775, whole genome shotgun sequence.
ACCESSION   DAOTKU010000385 DAOTKU010000000
VERSION     DAOTKU010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57942_length_1687_cov_1.998775"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1529)
                     /locus_tag="P5310_08010"
     CDS             complement(<1..1529)
                     /locus_tag="P5310_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU33338.1"
                     /translation="MKALESWNEKYDLDVCMIKEPFHSPGYHTTLKGGFVHPTRESLT
                     YAVALLDSNIEEYRLRAIDILKKVISLQDKEPGSPTYGIWSWFYEEPLDKMSPPDWNW
                     ANFCGKELLQVALDHFDKLPEVLREEIKNSIHYACHSIVRRNVGPSYTNIAIMGAYVT
                     LLSGELFGWEDIFNYGKDLLTRIYKYTQHHGNFTEYNSPTYTMVAIEDISRIISHIKD
                     SGCISMADYLNDIAWKCVALHFHPPTGQWSGPHSRCYNNLQGIVFWSRIYLATNGRVR
                     LLEGKELYSDINWHRIKMRCPDKYMDYFLHLNESREVREIFYKGDEELSPEIRAIIGN
                     PSSPTLVATTYLSPFYCTGSFYKTDFWNQRRGLIGYWGDREKPGYFQLKCLHDGYDYS
                     SGLFHSVHLKNGILGIINFATDYGDTHISLDKVRDATIKAEDLRLRLEFGGYTGDTNL
                     SKYSKLKDTFIIQRDGVTVALRILSASFGRYPLRLEIGEDERNKWCDLVFYSGEETSI
                     C"
BASE COUNT          531 a          359 c          267 g          530 t
ORIGIN      
        1 aaacagatag aagtttcttc accgctatag aataccagat cacaccattt atttctctca
       61 tcctctccaa tttctaatct cagaggatat cttccaaagg aagctgatag aattctcaag
      121 gctactgtta ctccatctcg ctggataata aaggtatctt tcaatttaga gtatttcgat
      181 agatttgtat ctcctgtata tccaccaaac tctaatctaa gccttaaatc ctcagccttt
      241 atagtagcat ctcttacttt atccaggctt atgtgagtat ctccatagtc agtagcaaaa
      301 tttataatac cgagtatacc atttttcaga tgaacagaat gaaaaagtcc agaagaataa
      361 tcatatccat catgcaggca tttaagttga aaataaccag gtttctctct atctccccaa
      421 taacctatca atcctctcct ctgattccag aaatctgtct tataaaaact acccgtacag
      481 tagaacggtg aaagataggt tgtagcaacc agagtgggag aagaaggatt gcctattata
      541 gcccttatct cgggacttaa ttcttcgtct cctttataga aaatctctct gacctcccta
      601 gattcattta gatgaaggaa ataatccata tatttgtcag gacatctcat ttttattcta
      661 tgccagttaa tatcagagta aagttctttt ccctctaaaa gtctcaccct accgttagtt
      721 gcaagatata tccttgacca aaagacaata ccctggagat tgttataaca tctactgtga
      781 ggtcctgacc attgccctgt aggtggatga aaatgcagag caacacattt ccatgcaata
      841 tcgtttagat agtctgccat agaaatgcat ccagaatctt ttatatgcga aattattctg
      901 gatatatctt ctatagcaac catagtatag gtaggactat tgtattcagt aaagttacca
      961 tggtgctgag tatatttata aatcctagtt aagaggtctt tcccgtagtt aaatatatct
     1021 tcccagccaa ataactctcc agataataat gtaacataag ctcccataat agctatatta
     1081 gtataagaag gtccaacatt acgtctgaca atagaatgac aggcataatg tatagaattc
     1141 tttatctcct ctctcagaac ttctggtagt ttatcgaagt ggtccagtgc aacctgcagt
     1201 agctcctttc cacagaaatt agcccagttc caatcaggag gagacatctt atccagaggt
     1261 tcttcataaa accatgacca gatcccgtaa gtcggactac cgggttcttt atcctgtaaa
     1321 gatattacct tcttcaggat atcaatagcc cttaatctat attcttctat attactatcc
     1381 agtaaagcaa cagcataggt taaagactct cttgtaggat ggacaaaacc tcccttcaaa
     1441 gtagtatgat atccaggaga atgaaatggc tcttttatca tgcatacatc aagatcatat
     1501 ttttcattcc agctttccag tgctttcata aatctagagt attcagattg ggacatctga
     1561 gacatatctc accatcctta aataaaatta ggggggctat aagcccccta tttacgtaaa
     1621 catttacttc ttgaaaaaga atatctgggg ctgagcatta cctggacttc tcaggatatc
     1681 gtcactt
//
LOCUS       DAOTKU010000386         1680 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_58268_length_1680_cov_2.272615, whole genome shotgun sequence.
ACCESSION   DAOTKU010000386 DAOTKU010000000
VERSION     DAOTKU010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58268_length_1680_cov_2.272615"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="P5310_08015"
     CDS             complement(<1..655)
                     /locus_tag="P5310_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU33339.1"
                     /translation="MNTVEEIKKRLTFSLKREANSDNFISENFIRELASSSVKLHKDI
                     SVFLSRKKQIIDVTINGGIVTLSSRQDKNVLSKVFCINTKFGKDARISGIDLATLKNL
                     KLDFLAILTILKDGRPGEMQVAFLSGERLDKFEFIGPYPYEYFLTFDFLGKISETEQD
                     AKTDVYNTEPERERVVLVDVRNGKDKEFEDYLFDELERLTYTASGEVVYKLYQKKGSP
                     "
     gene            complement(666..1412)
                     /locus_tag="P5310_08020"
     CDS             complement(666..1412)
                     /locus_tag="P5310_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016483798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HRU33340.1"
                     /translation="MSRIPIALQLYSVREECAKDFVGTVKAVSEMGYDGVEFAGYYNL
                     SASELKSILDDFGLKVAGTHIGINTLIGDELQRTIEFNATLGNKFLIVPALPPDYTGT
                     KEGWLKAAEMMNEISEKIKPYGMKVGYHNHDIEFKKIDGETPWDIFFQNTSEDVVMQL
                     DIGNAIHGSADPIEILKKYPGRAITVHLKEYPFSAPAVGEGEVNWKEIFNICETIGGT
                     EWYIVEYETPGRPPLESVRRCIENLRRMGK"
     gene            complement(1443..>1680)
                     /locus_tag="P5310_08025"
     CDS             complement(1443..>1680)
                     /locus_tag="P5310_08025"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF021883.2"
                     /note="Catalyzes two discrete reactions in the de novo
                     synthesis of purines: the cleavage of adenylosuccinate and
                     succinylaminoimidazole carboxamide ribotide; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HRU33341.1"
                     /translation="RVLLALMRKGLPRDIAYEMVQRNALKAWDLEKPLYELLVEDEEV
                     RKYLTEEDLKSSFTDEEYKKNLDIVYKRVGIDDH"
BASE COUNT          476 a          407 c          239 g          558 t
ORIGIN      
        1 ccggagaacc cttcttctga tagagtttat aaacaacctc tccactggca gtgtaggtta
       61 acctttctag ctcatcaaag agataatctt caaactcttt atctttaccg tttctcacat
      121 ctacaagtac taccctttct ctctctggct cagtattata tacatctgtc ttggcatctt
      181 gttcagtttc ggatatcttt cctagaaagt cgaacgtaag aaagtattca tacggatatg
      241 gtccgatgaa ttcaaattta tcaagtcttt ctccactaag aaaagcaacc tgcatctcac
      301 caggtctacc atcttttaag atagtgagga ttgcaagaaa gtcaagtttt aagtttttaa
      361 gtgtagcaag atcaatacca cttatccttg catccttacc aaatttcgta ttgatacaaa
      421 aaacttttga taagacattc ttatcctgtc gagaggataa ggtaactatt cccccattaa
      481 tagtaacatc tattatctgc ttcttcctgg agagaaagac agagatatcc ttatggagtt
      541 ttacagatga agatgccagt tctcttataa aattctcgct tataaagttg tcgctattag
      601 cctccctctt taaggaaaac gttaacctct tctttatctc ctcaactgta ttcataaaac
      661 ttgagctact ttcccatcct cctcaggttt tctatacaac gtctaaccga ttctaaagga
      721 ggtctacctg gtgtttcgta ttccactata taccattcgg tcccccctat tgtttcacag
      781 atgttaaata tctctttcca gttaacctct ccttcaccga cagctggagc gctaaacgga
      841 tactccttaa gatgaacagt aattgctctt ccaggatatt tcttaagtat ctctatcgga
      901 tcagccgaac catgtatggc attcccgata tctaattgca taacgacatc ttcactcgta
      961 ttctggaaaa atatatccca gggggtttct ccatctattt ttttaaactc aatatcatga
     1021 ttatgatagc caaccttcat tccatagggc tttatctttt cagatatctc attcatcatc
     1081 tccgctgcct taagccaacc ttctttagtc ccggtatagt ccggtggtaa tgccggaacg
     1141 ataaggaatt tgttacccaa tgtggcatta aattcaatcg tcctctgaag ttcgtcccca
     1201 attaaggtat taattccaat gtgagttcca gccactttca agccaaaatc atccaggata
     1261 ctctttaatt cactggcgct taaattataa tatcctgcaa actcaacccc atcatacccc
     1321 atttctgaaa cagcctttac tgtacccaca aaatctttag cacattcttc tcttaccgaa
     1381 taaagctgca gagctatagg tattctactc atttttacct cctgataaat gtttttactc
     1441 atctaatggt catcaattcc gaccctttta taaacaatat ctaaattctt cttatactct
     1501 tcatcagtaa aagaagattt cagatcttcc tctgtaagat actttcttac ctcctcgtct
     1561 tcaaccagaa gttcgtataa aggcttctct aaatcccagg ctttaagagc attcctctgg
     1621 accatctcat atgcaatatc tcttggaagt cccttgcgca taagagcaag caagaccctc
//
LOCUS       DAOTKU010000387         1679 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_58300_length_1679_cov_2.807882, whole genome shotgun sequence.
ACCESSION   DAOTKU010000387 DAOTKU010000000
VERSION     DAOTKU010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58300_length_1679_cov_2.807882"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            173..1234
                     /locus_tag="P5310_08030"
     CDS             173..1234
                     /locus_tag="P5310_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012366468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HRU33342.1"
                     /translation="MKAIVTHGPFDYRLETVPDPVPGPEEVVIRVKASGLCASDMKCY
                     HGGPSFWQGNPPYVKPPVIPGHEFIGEVVALGEGAEEKYGLKIGDKAVAEQILPCWEC
                     RFCKTGKYWMCERNYVFGFQGGIDDGGMAEYSKFPVGSIIHKVPKELPDEYGALIEPL
                     ACAIHAVERGEIQLGDTAVISGMGPIGLMMLQIAKLKNPGLLIALDTRDFRLDIAKKL
                     GADITINVAEEDAVKKVKDLTDGYGCDVYIEASGYPKSVPQGLDMIRKLGTFVEFGVF
                     GEPAVADWTIIGDRKELNIHGSHLGPYRYPLAIDYLTKGLIRGDLIVSHKIPLENFKD
                     AIKIAEDPNSNSIKILIIP"
     gene            1245..>1679
                     /locus_tag="P5310_08035"
     CDS             1245..>1679
                     /locus_tag="P5310_08035"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRU33343.1"
                     /translation="MFVRWRMTPNPIVIDPETSILDALHIMKEKKVRRLPVVAHGKLV
                     GIVTEKDLRESPSLKATSLSIFELNYLLAKTPVSEVMTKDPITVTPDTTIEEAAVIMR
                     DNQISGLPVVEDGKIVGIVTETDIFDMLVKLLGFRNGARFTLH"
BASE COUNT          533 a          284 c          419 g          443 t
ORIGIN      
        1 cccctggaat gggacatata gattttaaag atatattcag ggtactgtcg gttatcgatt
       61 acaacggatt tatatctgct gagcttataa ataagccaag tgtggagtca tctattaccc
      121 aaacgatgaa atatttaaag ggtatactat aaaaatttta ggaggtaaaa gtatgaaagc
      181 cattgtaact catggtccat ttgattatag acttgagact gttcctgacc ctgttcctgg
      241 accagaagag gtggtaatta gagtaaaggc aagtggactc tgtgcaagcg acatgaaatg
      301 ctatcacggt ggtccatcct tctggcaggg taatccccca tatgtaaagc caccagttat
      361 ccccggtcat gaatttatcg gagaagtggt ggctttgggg gaaggggctg aagaaaagta
      421 tgggcttaag ataggagata aagctgtagc ggaacagatt cttccctgct gggaatgcag
      481 gttctgtaaa acaggcaagt actggatgtg tgagaggaat tatgtgtttg gttttcaggg
      541 tggtatagat gatggtggta tggcagaata ctcaaagttt ccagttgggt ctattataca
      601 taaggtaccc aaagaattgc ccgatgaata cggagccctt atagaaccgt tggcatgcgc
      661 aatacatgcg gttgagagag gagagataca gcttggggat actgctgtta tctcaggtat
      721 gggacccatc ggtcttatga tgctgcagat tgcaaaatta aagaatcccg gacttctcat
      781 agcgctggat acaagagatt tcagactgga tatagcaaaa aaactgggag cagatataac
      841 gatcaatgtg gctgaggaag atgcggtaaa aaaggtaaaa gaccttacag atggctatgg
      901 ctgtgatgta tatatagagg ccagcggtta tccaaaatcg gtacctcagg gtctggatat
      961 gatcagaaaa ctcgggacat ttgttgagtt tggagttttt ggtgaaccgg cggttgctga
     1021 ctggacgata ataggtgaca ggaaagaact aaatatacat ggttcccacc ttggtccata
     1081 cagatatcct cttgcaatag attatcttac aaaaggtttg ataagaggag accttattgt
     1141 ttcccataaa attccattag agaactttaa agatgccata aagattgcag aagacccaaa
     1201 tagtaactca ataaagattc taattattcc ataaggaggg tcagatgttt gtaagatgga
     1261 gaatgactcc aaatcctata gttatagacc cagagacaag tatacttgat gcccttcata
     1321 ttatgaaaga gaaaaaggtg aggagactac cagtagttgc acatggaaag cttgtcggta
     1381 tcgttactga gaaagatctg agagagtccc cgtccttaaa ggctactagt ctgagtatat
     1441 ttgaactaaa ctatctcctt gccaagaccc cggtaagtga ggttatgact aaggacccca
     1501 tcacagttac gcctgatacc acgatagaag aggcggctgt aataatgaga gataaccaga
     1561 taagtggact cccggtggta gaggacggaa agatcgttgg catagtaacc gaaacagata
     1621 tatttgatat gcttgtaaag ctgttaggat tcagaaatgg agccagattt acacttcat
//
LOCUS       DAOTKU010000388         1675 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_58494_length_1675_cov_3.374074, whole genome shotgun sequence.
ACCESSION   DAOTKU010000388 DAOTKU010000000
VERSION     DAOTKU010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58494_length_1675_cov_3.374074"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1152)
                     /locus_tag="P5310_08040"
     CDS             complement(<1..1152)
                     /locus_tag="P5310_08040"
                     /inference="COORDINATES: protein motif:HMM:NF023169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HRU33344.1"
                     /translation="MKTKIVFILCLLLILSSVNISLGKSLADVSWHIEGDRLIISLRA
                     PDRIAYTTFTLSDPVRFVIDVYNVTIPDPKSIEINKSPVKSIRMAPKDEGRLRIVLET
                     VKMPEYNINLSKNEKELFAEFKLNVSYTSNSIRKVLYGNDAVSIFFNTTLSRYEWILD
                     DNPPRFIMEFPLTSLTGKIERVSDSSVVNRILGYDYEDREKNINTVLIVELKEKIAPL
                     IVRDQDNNIIRLNFKREEVSSAQTKTVPSPTASTTPKEQTVKVSPGEKRVSLNFKDAD
                     IKDVLQALSMKAGVNIIVDEGVSKKITLTLNNVTAREALEMITKASGLAYERWNNGYI
                     VASPVRLLEILDSGMAKVKQTVESIEIRGDIVQIQNTLKVVYPDIVTSVS"
     gene            complement(1149..>1675)
                     /locus_tag="P5310_08045"
     CDS             complement(1149..>1675)
                     /locus_tag="P5310_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU33345.1"
                     /translation="QEIPSIPPPVPELPEKTVQSVFYYPPEIYRDPFKVPIRIKPLEE
                     VVKAEVPKQETSQEISAPGEEVSPAVIPVQKAQPVAESKPETVQTEGKVPVVTRTEEE
                     KIQQKISLPKVIVTGIIYDNEPFAILEFEGKSGIFEKGDKLSGDLSVKKIYADSVDID
                     WKGRVYNIKLGGKI"
BASE COUNT          480 a          355 c          267 g          573 t
ORIGIN      
        1 acttacactt gttactatat ccgggtatac taccttcaac gtgttctgta tctgcactat
       61 gtctcctctt atctctatcg actctactgt ttgcttcact ttagccatgc cggagtctaa
      121 aatttccagc agtctaaccg ggctggctac tatataacca ttgttccatc tttcataagc
      181 taaacctgaa gccttggtta tcatttctaa agcttctcta gcggtgacat tatttaaagt
      241 cagggtaatc tttttagaaa caccctcatc cacgatgata tttactccag ctttcatcga
      301 caatgcctga aggacatctt ttatgtctgc atccttaaaa ttaagtgaca ctcttttctc
      361 accgggagaa acctttacag tttgttcttt aggagtagtc gaggctgttg gagaggggac
      421 agttttagtc tgggcagaag aaacctcttc cctcttaaaa ttcaatctta ttatattatt
      481 atcctgatcc ctcacgatta gtggtgctat cttctcttta agttctacga tcagtacagt
      541 atttatattc ttctctctat cctcataatc atacccaagg attctattca caacactgga
      601 atcagaaact ctttctatct ttccagtaag agaggttaaa ggaaactcca ttataaatct
      661 agggggatta tcatccagaa tccattcata tctagataag gtggtattaa aaaaaataga
      721 tacagcatca ttaccataga ggacctttct tattgaatta ctggtatagg aaacattcaa
      781 tttaaattcg gcaaagagct ctttctcgtt cttagataga ttaatattat attcaggcat
      841 cttaacagtt tccagaacaa ttcttaacct tccttcatct ttaggtgcca tccttattga
      901 ttttactggg gacttattta tctcaatact tttagggtca ggaatagtaa cattatagac
      961 atcaatcaca aaacggacag gatcagacag agtaaaagta gtataggcaa ttctatctgg
     1021 agctcttaaa cttattatta atctatcgcc ttctatgtgc cagctaacat cagctaaact
     1081 cttcccaaga ctaatattaa ctgaagagag aattaataat aaacataaaa taaaaacaat
     1141 cttagttttc atattttacc ccctagcttt atattataga ctctaccttt ccagtcaata
     1201 tctactgaat cagcatatat cttcttaacc gataaatctc cggataattt atcccccttc
     1261 tcaaatatcc cactctttcc ttcaaattcc aatatagcaa atggttcatt gtcgtagata
     1321 atacctgtta ctataacctt gggtaaagat attttctgct ggatcttttc ttcttcggtt
     1381 ctggtcacca ccggcacttt accctcagtc tgaacagttt caggctttga ctctgctaca
     1441 ggttgagctt tttgtacagg aataactgca gggctaactt cctccccagg ggctgatatc
     1501 tcttgggagg tttcctgttt aggaacttct gcctttacca cctcttcgag aggttttatt
     1561 cttattggaa ccttaaatgg atctctgtat atttcgggag gataataaaa gactgactga
     1621 actgtttttt ctggcaattc agggactgga ggaggaatag atggaatctc ttgaa
//
LOCUS       DAOTKU010000389         1655 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_59519_length_1655_cov_3.633125, whole genome shotgun sequence.
ACCESSION   DAOTKU010000389 DAOTKU010000000
VERSION     DAOTKU010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59519_length_1655_cov_3.633125"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="P5310_08050"
     CDS             <1..313
                     /locus_tag="P5310_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004400328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HRU33346.1"
                     /translation="FEISKLLGAAVGASRSVVDMGWVEYPHQVGLSGKTVNPRVYIAI
                     GISGAVQHLAGMMNSESIIAINKDPEAPIFQVADLGIVGDALEILPVLKKRLEERKRN
                     V"
     gene            306..>1655
                     /locus_tag="P5310_08055"
     CDS             306..>1655
                     /locus_tag="P5310_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004400326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRU33347.1"
                     /translation="MFNKVDERIIDNLIEICGTNDVLIEEEELERYSQDESGRFYAHM
                     PEVVIRAENTEEISKVLKLANENLIPVTPRGAGSGVVGGAVPVYGGILLSLEKMNQIL
                     EIDKVNLVAMAEPGVVTNDLCKKVAEEGLFYPGYPMSVETSFLGGNVATNAGGSKVIK
                     YGNTGHHILGLEVVFPSGEVLPLGGKRRKDSSGYNLLNLMVGSEGTLGVFSKITVNLI
                     PLPLYTVDLLVPFSTVESAIQTVPEVIRESKVLPCAVEFLDRPSVELASIYTKCRWEY
                     QEQAEAYLLIQFDGNSKVQLEESYIRAGEACLKFGALEVFVAENPFASERLWRLRRNY
                     LEAIKAVDPYVVTGDAVVPTSLIPRMLKRIKEIGDEYGTDILCAAHAGDGNIHSAPLR
                     PENIPPEEWQSIQEEILDKIAVEASSLGGAPSGEHGIGYVKKKALYISKKREIPVMRE
                     "
BASE COUNT          532 a          263 c          446 g          414 t
ORIGIN      
        1 atttgaaatt tctaagctat tgggggctgc ggtaggagcc tccagatcag tggtggatat
       61 gggatgggta gaatatcctc atcaggttgg tctgagcggg aagacggtca atcccagggt
      121 atatattgct attgggatat caggagcggt acaacatctg gctggaatga tgaactctga
      181 aagtataatt gcgataaata aagatccgga agcaccaata tttcaggtgg cagatctggg
      241 aatagtagga gacgcattgg aaatactccc tgttctgaag aagagactgg aggagagaaa
      301 gagaaatgtt taataaagtc gatgaaagaa ttatagataa cctgatagag atctgtggga
      361 cgaatgatgt acttatagaa gaggaagaat tggaaagata ttcccaggat gaatctggta
      421 gattctatgc ccatatgcca gaagtagtaa tcagagccga aaatacagaa gagatatcta
      481 aggtcctgaa acttgccaat gaaaatctca taccggtaac cccgagaggt gcaggaagcg
      541 gggtagtagg gggagcggtc cctgtatacg gtgggatact actttctctt gagaagatga
      601 atcagatctt ggagatagat aaggtcaacc tggtggctat ggctgaaccg ggagtagtga
      661 ctaacgatct ctgcaaaaaa gtggctgaag aaggtttatt ctatccagga tatccgatga
      721 gtgtggagac gagtttcctg gggggaaatg tggcaacaaa tgctggtgga agcaaggtaa
      781 taaagtatgg aaatactgga caccatatcc tggggcttga agtagttttt ccctcaggag
      841 aggtccttcc ccttggtgga aagagaagaa aagactcaag tggatataac cttttaaatc
      901 ttatggttgg ctcagaagga acccttgggg tattcagtaa gatcactgta aatcttatac
      961 ctcttccact ctataccgta gacctccttg ttcctttttc aacagtagaa tctgctatac
     1021 aaactgtccc agaggttata agagaaagta aggttttacc ctgcgctgtt gagtttctcg
     1081 atagaccatc agtagagctg gcgagtatat atacaaagtg cagatgggag tatcaggaac
     1141 aggcagaggc atatcttctc attcaatttg atgggaacag taaagtgcag ttagaagaat
     1201 cgtatataag agctggagaa gcctgtttaa agtttggagc attagaggtc tttgtggcag
     1261 aaaatccatt tgcatctgag agattatgga gactcaggag aaattatctg gaggctataa
     1321 aagcggtgga tccatatgta gttactggag atgcagtggt ccctacctct ttaattccca
     1381 gaatgttaaa gagaataaag gagataggag atgaatatgg aacagatata ctatgtgcag
     1441 cacatgcagg tgatggaaat atacactctg ctcccctaag acctgagaat attcctccag
     1501 aagaatggca atcaatacag gaagagattc tagataagat agcggtagag gctagctcac
     1561 ttgggggagc tccaagtgga gaacatggaa tagggtatgt aaaaaagaag gcgttgtata
     1621 tatcaaagaa gagagagatt cctgtgatga gagag
//
LOCUS       DAOTKU010000390         1642 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_60177_length_1642_cov_3.210460, whole genome shotgun sequence.
ACCESSION   DAOTKU010000390 DAOTKU010000000
VERSION     DAOTKU010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60177_length_1642_cov_3.210460"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /locus_tag="P5310_08060"
     CDS             complement(<1..729)
                     /locus_tag="P5310_08060"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRU33348.1"
                     /translation="MSNKPKVLLAMHRNLGEQIFNKKDLEYFKTFVEVNNVGSLPEKI
                     DESFIKDNIKGVDICVTCWGTPPFGKEILDNADNLKLIAHSAGSVKPIVTDYVWERGI
                     RVTSAAPAIAVAVAEMTLGLMIISLRRIFHFNELTHKGFWKSDEEISKTRSLYCVTVG
                     VVGASNVGRNVIKLLKNFQVEILLYDPYVTQEEADKLGVKLVSLEELMSKSDVVSLHA
                     PSIPETYHMINKNNISLLKPGSIII"
     gene            complement(810..>1642)
                     /locus_tag="P5310_08065"
     CDS             complement(810..>1642)
                     /locus_tag="P5310_08065"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRU33349.1"
                     /translation="QLRKLFRDKRFRLALSLAVDRDAISKILYAGQSEKRLLVMPESS
                     PYYSPEQLKSASKYVDYDPKKANQLLDQLGLTKRDKNGIRLFPDGTPVEILVEYRHSP
                     GSYQGDTPELIAKYLTDVGIKVALKYESSSLLSTRTAANEHQVVAINISGPWHSILGY
                     GFLPSSNPTTLYWAPLWYRWFISNGKEGEEPPAVFKKLVTLGDGILGGLTIEARRKMV
                     TEILRLHNENLWIIPITTYPGEAHVIKKNFRNTPRKVTGLQNFYHRQPLYPEQFFIKQ
                     "
BASE COUNT          491 a          326 c          280 g          545 t
ORIGIN      
        1 tataattatg gaacctggct ttaagagact tatattattc ttatttatca tatggtatgt
       61 ctcagggata cttggggcat gaagagatac tacatctgat tttgacataa gttcttctaa
      121 agatacaagt tttactccaa gtttatctgc ttcctcttga gttacatagg ggtcataaag
      181 gagtatctct acctgaaagt tctttaagag ttttataaca tttctaccta cattgctggc
      241 acccactaca cctactgtaa cacagtagag ggacctagtc ttggatatct cttcatctga
      301 tttccaaaac cctttatgag taagttcatt aaagtgaaat atcctccgta aagatataat
      361 cataagccct aaagtcatct ctgctaccgc aactgctata gccggggcag cactagttac
      421 tctaatacct ctttcccata cataatctgt tactatgggt ttaacagaac cggcagagtg
      481 tgctataagc tttaaattat cagcattatc tagaatctct ttaccaaaag gaggggtccc
      541 ccaacaggta acacatatat ctacaccttt tatattatct tttataaatg actcatctat
      601 cttttctgga agagaaccaa cattgttaac ttctacaaat gtcttaaaat actcgagatc
      661 ttttttgtta aagatctgtt ctccaaggtt tctatgcatt gctaatagaa ctttaggttt
      721 gttagacata attttcaact cctttcaagt aataatatcc ccccagacaa tctagctatt
      781 agtcttgaat tgtctggggg aaattaaatt tattgcttta taaagaactg ctcaggatat
      841 agaggttgac ggtgataaaa attctgtaaa ccggtaactt ttctgggagt attcctgaaa
      901 ttcttcttta ttacatgagc ttcacctggg taagtggtta taggtattat ccagaggttc
      961 tcgttatgga gtctaagaat ctctgtaacc atctttcttc tagcttcaat agtaagtcct
     1021 cctaagattc catcccctaa tgttacaagc ttcttaaaga ctgcaggagg ctcttctcct
     1081 tctttaccgt tagaaataaa ccatctatac catagaggag cccagtatag agttgtagga
     1141 tttgaactcg gaagaaatcc atatcctagt atagaatgcc agggtccact tatgttaatc
     1201 gctactactt gatgttcatt ggcagcagtt cttgtggaaa gtagtgatga gctttcatat
     1261 ttcaaggcta ctttaattcc aacatcagtt aaatacttag ctattaattc cggagtatca
     1321 ccctgatagg aaccaggtga atgacgatat tccaccagta tctcaaccgg agtcccatcc
     1381 ggaaagagtc taataccatt tttatctctt ttagtaagac ccaactgatc taacaattgg
     1441 ttagcttttt tgggatcata gtctacatat tttgatgcag atttaagttg ttctggagaa
     1501 taataaggag aactttcagg catcaccaaa agtcttttct cactctgtcc agcataaaga
     1561 atcttactta ttgcatctct atcaaccgca agagataagg ctaatctaaa gcgtttatca
     1621 cggaagagct tacgaagctg cg
//
LOCUS       DAOTKU010000391         1641 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_60249_length_1641_cov_2.439470, whole genome shotgun sequence.
ACCESSION   DAOTKU010000391 DAOTKU010000000
VERSION     DAOTKU010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60249_length_1641_cov_2.439470"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="P5310_08070"
     CDS             <1..229
                     /locus_tag="P5310_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU33350.1"
                     /translation="IPLYKNPIFSHHISPEMILAITKELFGVSPAAYLVSVRGYSFDF
                     GFEITREAQENSEKAIDRIKEICMNWLSQKR"
     gene            199..558
                     /locus_tag="P5310_08075"
     CDS             199..558
                     /locus_tag="P5310_08075"
                     /inference="COORDINATES: protein motif:HMM:NF013334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HRU33351.1"
                     /translation="MHELAVTEEIIKVVTDASKGRRIREIVLVIGNLSSFIDESIQFY
                     FDKLTESTPLEGAKLSFKRIPVKLRCYRCREEFIPDEEYICPYCKSIGGDIIEGREFY
                     IESIEVEDEDRDSKEYT"
     gene            527..1171
                     /gene="hypB"
                     /locus_tag="P5310_08080"
     CDS             527..1171
                     /gene="hypB"
                     /locus_tag="P5310_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HRU33352.1"
                     /translation="MKIEIVKNILEANDQVALLNRETFNNLNVFTVNVMASPGAGKTS
                     LILETIPRLNSSVAVIEGDVASSIDSEKVARFNIPVVQINTGGNCHLDASMIREAIKK
                     LSLEGIRYLFIENIGNLICPASFDLGEHIRIVISSVPEGDDKPYKYPGLFSTVDAVIL
                     NKIDLLPYVDFNIEYFKNGITAINKKAKIFPVSCKTDEGIDSWVEWLKGENRYK"
     gene            1164..>1641
                     /locus_tag="P5310_08085"
     CDS             1164..>1641
                     /locus_tag="P5310_08085"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012582504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HRU33353.1"
                     /translation="MNSKHIRVYGIVQGVGFRPFIYRLAKKYNLKGWVLNTSSSVEIE
                     VEGEEDSIKAFINDIQTKKPPLARIERIETEDIHFNNFEDFIIKESKEDSGYQLISPD
                     IAVCSDCLRELFDPNDRRYRYPFINCTNCGPRFTIIEDIPYDRKNTTMKKFKMCRDC"
BASE COUNT          584 a          245 c          372 g          440 t
ORIGIN      
        1 aatccctcta tataagaatc ctatcttttc ccatcacata agtcctgaga tgattctggc
       61 tataactaaa gaattatttg gggtttctcc tgctgcgtat ttagtatctg taagaggata
      121 tagcttcgac tttggttttg aaatcaccag agaggctcag gaaaattcgg aaaaggctat
      181 agacagaata aaagagatat gcatgaactg gctgtcacag aagagataat aaaagttgta
      241 acagatgcaa gcaaagggag aaggataagg gagatagtgc tggtaatagg gaatttatcc
      301 agttttatag atgagtccat acaattttat tttgataaat tgactgagtc caccccttta
      361 gaaggggcta agctttcatt taaaaggatt ccagtaaaac tgagatgtta tagatgtagg
      421 gaggaattta tacctgatga ggagtatata tgcccgtact gcaaaagtat cgggggagat
      481 ataatagaag gtagagaatt ctacatagag agtatagagg tagaagatga agatagagat
      541 agtaaagaat atacttgagg caaatgatca ggtagctctt ttaaacaggg agacctttaa
      601 taacttaaat gtatttactg taaatgttat ggcatcaccc ggagctggga agacaagttt
      661 gatattagag accattccca gattaaatag ttcagtagct gttatagaag gtgatgttgc
      721 atccagtata gacagtgaaa aggtagcgag gtttaatatt cctgtcgtac agataaatac
      781 cggagggaac tgtcatctgg atgcctctat gataagagag gcgataaaga agctttccct
      841 tgagggaata agatatctat tcatagaaaa tataggaaac cttatctgtc cggccagttt
      901 tgacctgggg gaacatataa ggattgtcat atcaagtgta ccggagggtg atgacaagcc
      961 atataaatat ccaggactat tctcaacagt ggatgcagta atcttaaata agatagacct
     1021 cctcccatac gtagatttca atattgaata cttcaagaat ggtataacgg cgataaataa
     1081 aaaggctaaa atcttcccag tatcctgtaa gacagatgaa gggatagatt cctgggtaga
     1141 atggttgaag ggtgaaaata ggtataaata gtaaacatat aagggtgtat ggcattgtcc
     1201 agggagtagg attcagaccc ttcatctaca gattagccaa aaaatataat cttaagggat
     1261 gggtattgaa cacttcaagt tcggtggaga tagaggtaga gggagaagag gatagtataa
     1321 aagcctttat aaatgatata cagacaaaaa aacctcctct ggcaagaata gagcgtatag
     1381 agacagaaga tatccacttt aataattttg aagatttcat cataaaagag agtaaggagg
     1441 actctggcta tcaattaata tctccggata tagctgtctg cagcgattgt cttagagagc
     1501 ttttcgatcc caatgacaga agatacagat atccatttat aaactgcaca aactgcggtc
     1561 caagattcac cattatagaa gatatcccct acgatagaaa gaataccacc atgaaaaaat
     1621 tcaagatgtg cagggactgt g
//
LOCUS       DAOTKU010000392         1620 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_61358_length_1620_cov_2.851118, whole genome shotgun sequence.
ACCESSION   DAOTKU010000392 DAOTKU010000000
VERSION     DAOTKU010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61358_length_1620_cov_2.851118"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..141)
                     /locus_tag="P5310_08090"
     CDS             complement(<1..141)
                     /locus_tag="P5310_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013251808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRU33354.1"
                     /translation="MTEVELIIVGGGPAGLTSAIYSARALIDTLVIEKMLPGGQPVLT
                     TFI"
     gene            complement(138..482)
                     /gene="trxA"
                     /locus_tag="P5310_08095"
     CDS             complement(138..482)
                     /gene="trxA"
                     /locus_tag="P5310_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HRU33355.1"
                     /translation="MNLKNSYNRVKAVSSADFDSEVINSSMPVLVDFWADWCMPCKML
                     SPIVEEVAEELADRLKVVKVNVDENPDLAMSYGVQAIPTLLVFKDGRVVTETVGYLSK
                     KALMDKLGEVIK"
     gene            complement(439..864)
                     /locus_tag="P5310_08100"
     CDS             complement(439..864)
                     /locus_tag="P5310_08100"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRU33356.1"
                     /translation="MKRSIFISSIILLTVLSLGISLSYGKSIKEKLPEERVNELIKNK
                     KPFMIDFYADWCPPCNAMKPVLEELGKKYKGRVEIVRVNVDMPENNALVVKHRIVSIP
                     TFIFINSKGEISNKIIGYSDMEVMENEFKKLLQPGKSGK"
     gene            complement(861..1115)
                     /locus_tag="P5310_08105"
     CDS             complement(861..1115)
                     /locus_tag="P5310_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019123034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HRU33357.1"
                     /translation="MDKSIHNRLARIEGQMRGIEGMIEEGRSCKEVLSQLLAVRSAVD
                     SLIVLILRDRLEKCRTEEFDLEDIDEILDLLVRCLSEVKE"
     gene            complement(1176..>1620)
                     /locus_tag="P5310_08110"
     CDS             complement(1176..>1620)
                     /locus_tag="P5310_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRU33358.1"
                     /translation="LDQEVVELMAAGSCLVDPEIGKAGDIDTAIVTLKYEKGAWGIID
                     NSRKAVYGYDQRIEIFGSKGCIMVGNPKNSEVMISDADGIKTDKPLFFFVERYKEAYL
                     AEMEEFIRCILEDQEPPAGGWDGKVAVKMGYAARESLSKGCFVKI"
BASE COUNT          456 a          432 c          222 g          510 t
ORIGIN      
        1 tatgaacgtg gtaagaacag gctgaccccc aggtagcatc ttctcaataa ccagggtatc
       61 tataagggct ctagcagaat agatagcaga ggttaatccc gcaggacctc cgcctactat
      121 aataagctct acctcagtca ttttataact tctcccaatt tatccataag agccttctta
      181 gagagatagc ccaccgtctc tgtaacaacc cttccatctt taaagactaa aagtgtgggt
      241 attgcctgta caccataact catagccaga tcaggatttt catcaacgtt taccttgacc
      301 actttaagtc tgtcggcaag ttcttctgcc acctcttcca ctattggact taacatttta
      361 cagggcatac accagtcagc ccaaaaatca accagaacag gcatactgga atttatcact
      421 tcagaatcaa aatctgcact acttaccgct tttacccggt tgtaagagtt ttttaaattc
      481 attttccatt acctccatat cgctatatcc tattatttta ttacttattt cgcccttact
      541 gttaatgaat ataaaagtgg gtatactgac gattctgtgc tttaccacaa gagcgttatt
      601 ctccggcata tccacattta cccttactat ctccacacga cctttatact tctttcccag
      661 ctcctccagt acaggcttca tagcattaca gggaggacac cagtcagcat aaaaatctat
      721 cataaaaggt tttttattct ttataagttc atttaccctt tcctcaggta acttctcctt
      781 aatactcttt ccataggata aacttatgcc aagagataaa accgttaaca ggataatact
      841 cgaaataaaa atgcttctct tcattctttc acctccgata ggcaccttac taaaaggtcc
      901 agaatctcat ctatatcttc caggtcaaac tcctcagtcc tgcatttctc caggcgatct
      961 ctaagtatca ggactattaa actgtccact gcagacctga ctgcaagtaa ctgagacagg
     1021 acctctttac aggacctacc ttcctctatc atcccctcta tccccctcat ctgtccctct
     1081 attcttgcca atctgttatg tatactctta tccataaata taccccctag gggtatataa
     1141 taacatattg aaaaataaaa agcaatataa tttatttata tttttacaaa acagccctta
     1201 ctcagggact ctctggctgc atatcccatc ttaacagcta cctttccgtc ccatccacct
     1261 gccggtggtt cttgatcctc caggatgcat cttataaact cttccatttc agccagatat
     1321 gcttccttat acctttcaac aaagaagaaa aggggtttat cagtcttaat accatcggca
     1381 tcgctgatca ttacttcact attttttgga ttacctacca ttatgcaacc ttttgagcca
     1441 aagatttcta ttctctgatc ataaccatac accgccttac gactattatc gataattccc
     1501 catgcacctt tttcgtattt cagggtaact attgctgtat caatatctcc tgctttccca
     1561 atttctggat caaccagaca gctgcccgct gccataagct ccaccacttc ctgatccagg
//
LOCUS       DAOTKU010000393         1618 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_61466_length_1618_cov_4.015355, whole genome shotgun sequence.
ACCESSION   DAOTKU010000393 DAOTKU010000000
VERSION     DAOTKU010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61466_length_1618_cov_4.015355"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..831)
                     /locus_tag="P5310_08115"
     CDS             complement(<1..831)
                     /locus_tag="P5310_08115"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HRU33359.1"
                     /translation="MRILYLDIDTLRPDHLSCYGYHRETSPNIDSVAREGMIFERFYA
                     SDTPCMPSRAALFTGRFGIHNGIINHGFTASDIKPFEPLRFFNNRQGPLTQLVTLLRR
                     LGYYTVSISPFGERHSAWWYYDGFREMYNSGKGGMETADEVMPLALDWLEKNGKDDNW
                     FLHINIWDPHTPYRTPLNYGNPFDDAPHPSWLKEETLRRHREMYGPHSARTPHDLRDM
                     PGDFFDRYPRLPRSIETPKDYKKWIDGYDTGINYADYWSGKVFDLLKKSGVWEDTLII
                     I"
     gene            complement(828..1601)
                     /locus_tag="P5310_08120"
     CDS             complement(828..1601)
                     /locus_tag="P5310_08120"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRU33360.1"
                     /translation="MFICTPPYSREPENIIKSARRGIHLFIEKPPARTLITGERLQDV
                     FDKTGIVVSVGFLWRYSEFSSKVKEYLKDERCAMIVSRWYHTIPPIAWVRDKERAGGQ
                     VVDQSIHLIDLMRYIVGDARSVYSQSSKGLFPEVEDFINDDASVTIINFQNGTVASLA
                     STYSLFPHPEHGARIEFMPKRLRLEGSLDYLKVFTPENMESFKFTDDPMEKEQLTFFE
                     AIEKNDRSKILSPYEDSLKTLKIALSANRSMETGEVINL"
BASE COUNT          442 a          355 c          291 g          530 t
ORIGIN      
        1 gattattatt aaagtatctt cccagacccc tgattttttt aataggtcaa agacctttcc
       61 tgaccagtaa tcagcatagt ttatccctgt atcatagcca tcgatccatt tcttataatc
      121 tttgggtgtc tcgatacttc tgggaagtct agggtatcta tcaaaaaaat cccccggcat
      181 atctctaaga tcgtgtggag tacgggcaga gtgaggtcca tacatctccc tatgccttct
      241 taaagtctct tcttttaacc aagaaggatg aggggcatca tcaaaagggt tcccataatt
      301 tagtggtgtc ctgtaagggg tatgggggtc ccagatgttt atatgtagaa accagttatc
      361 atcctttcca ttcttctcta accaatctag agctaaaggc ataacttcat ctgctgtctc
      421 cattccgcct ttgcctgaat tatacatctc tctaaatccg tcatagtacc accaggcaga
      481 atgtctctct ccaaaaggac ttatacttac cgtatagtag ccgagacgtc gaagaagagt
      541 gaccagttga gttaaaggtc cctgtctatt attaaagaat cttagaggct caaatggttt
      601 tatatcactg gctgtaaacc catgatttat aataccgtta tgaattccaa accttccagt
      661 aaagagtgcc gctcgcgaag gcatacaggg ggtatcagag gcgtagaatc tttcaaatat
      721 cataccttcc ctagctactg aatctatatt tggagatgtc tctctatgat atccatagca
      781 ggatagatga tcaggtctta aggtgtctat atcaaggtaa agtatcctca taagtttatc
      841 acctcgccag tctccataga tctatttgct gacaaggcaa tttttagtgt cttgagagaa
      901 tcttcatatg gggagagtat cttgcttcta tcattcttct ctatagcttc aaagaatgtc
      961 aattgttcct tttccatagg gtcatctgta aatttaaagc tttccatatt ttcaggggta
     1021 aataccttta gatagtccag gcttccttca agtctcaacc tcttaggcat aaactctatc
     1081 cttgctccat gttctgggtg aggaaacagt gaatatgtcg atgccaggct tgctactgtt
     1141 ccattctgga agtttataat ggttacactg gcatcatcat tgatgaagtc ctcaacttct
     1201 gggaataacc cctttgaact ttgagaatat acactcctgg catccccaac tatgtatctc
     1261 atcagatcta ttaggtgaat agattggtct actacctgcc caccagctct ttccttgtct
     1321 ctaacccagg ctattggtgg gattgtatga taccatctgg atacaatcat tgcacatctc
     1381 tcatctttca gatactcttt tactttactg gaaaactctg agtatctcca taaaaaacca
     1441 acacttacaa caataccggt cttatcaaat acatcttgca atctttctcc tgttatcaga
     1501 gtcctagctg gaggcttttc tatgaataga tgtatacctc ttctggcaga ttttattata
     1561 ttctcaggct ctctagagta tggtggtgta catataaaaa tggcatcaag attctttt
//
LOCUS       DAOTKU010000394         1614 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_61702_length_1614_cov_2.905709, whole genome shotgun sequence.
ACCESSION   DAOTKU010000394 DAOTKU010000000
VERSION     DAOTKU010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61702_length_1614_cov_2.905709"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1614)
                     /locus_tag="P5310_08125"
     CDS             complement(<1..>1614)
                     /locus_tag="P5310_08125"
                     /inference="COORDINATES: protein motif:HMM:NF019368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HRU33361.1"
                     /translation="RCSVAWLPDAFGFTWTLPQLFKKAGIDYFLTTKINWNETNKFPY
                     DLFYWQGLDGTRIIAHSFLNPGQGYNGNIQALDILGTWKNYRQKAVFPDTLLSFGFGD
                     GGGGPTLEMLEMYQRFKEFPGFPRLKMGSIEEFFARVPKDNLPVWDGELYLELHRGTY
                     TTQSRMKKSNRKVEHTLYTAEVLASINYLLTGKYPQDILTTGWHKLLRNQFHDILPGS
                     SIKEVYQDALRELKEVEDSADEIIRDSLKSIATRVKADREGFIIFNPSSFPRALEYII
                     EDDTDAVELPDGKVIKTQVTSGGKRLVYSPEVKVPPMGYVVLYKSEKTSLSNKTITSG
                     NILENNLVRVEIGEDGTILSYYDKVNGREVFTSRGNQLWAYSDRPRNWDAWDIAADYE
                     KYGEEIRDVEYIKILEEGPARGVIEVKKRYRNSSIIQRYVLYKDSPKLDIETIIDWHD
                     RRILVKALFPFDVRSSYATYEIAYGHIQRPTHKNTSWDSAKFEVPGHRWVDISEGNYG
                     ISILNDGKYGYSANESTIGLSLLRSPIYP"
BASE COUNT          418 a          398 c          299 g          499 t
ORIGIN      
        1 caggatatat aggagaacgg agcagagaaa gacctatggt actttcgttt gcgctgtacc
       61 catactttcc atcattcagt atacttatac cataatttcc ctctgagata tccacccatc
      121 tgtgtcctgg aacttcaaat tttgcagagt cccagctggt attcttatgt gtcggtctct
      181 gtatatgtcc gtaagctatc tcataagttg cataagaact cctcacatca aatgggaata
      241 gagcctttac aagaatcctc ctgtcgtgcc agtctattat ggtctctata tccagttttg
      301 gactatcctt gtacagtaca tatctctgaa taatagaaga gtttctgtat ctcttcttta
      361 cctcgattac tcctctggct ggtccttctt ccagaatctt tatatattca acatccctta
      421 tctcttctcc atacttttca taatcggcgg caatatccca ggcatcccag tttctcggtc
      481 tatcggagta tgcccacaac tgattgcctc tggaggtaaa gacctccctg ccattcacct
      541 tatcatagta tgagagaata gtcccatctt ctcctatttc aacccttacc aggttgttct
      601 ccaggatatt accagaggta atggtcttat tcgataaaga tgtcttctct gatttataaa
      661 gcaccacata tcccatcggc gggaccttaa cctcggggct atataccagt ctcttaccac
      721 cagaggtgac ctgtgtcttg atgaccttgc catcagggag ttcaacggca tcagtatcat
      781 cctcaataat atactctaga gctctgggga aagaactggg attaaagata ataaatcctt
      841 ccctatcagc ctttaccctg gtagctatac tctttaaaga atctcttata atctcatcag
      901 cagagtcttc cacctctttc agctctctaa gggcatcctg gtatacctct tttatcgaag
      961 aacctgggag tatatcatgg aactggtttc tgagaagttt gtgccaacca gtagttaaga
     1021 tatcctgggg gtatttgccc gtcagaagat agtttatact cgccaggact tctgctgtat
     1081 ataatgtatg ctccactttt ctatttgatt ttttcatcct ggactgtgtg gtatatgtcc
     1141 cacgatgaag ctcaagatag agctcaccgt cccatactgg tagattgtcc ttaggcactc
     1201 tggcaaagaa ctcttctata gaccccatct tcaacctggg aaaaccggga aattccttaa
     1261 atctctgata catctccagc atctccaggg ttggtccacc accgccatct ccaaatccaa
     1321 acgagagtaa tgtatcggga aatacagcct tctgtcgata gtttttccat gtgccaagta
     1381 tatccagagc ctggatatta ccgttataac cctgcccggg attgaggaat gaatgggcta
     1441 ttattcttgt cccatccagt ccctgccagt agaaaaggtc atagggaaac ttgtttgtct
     1501 cattccagtt tatcttggtg gtaaggaaat agtcaatccc tgccttttta aacagctgtg
     1561 gtaatgtcca ggtaaaacca aacgcatctg gaagccaggc aacactacat cttc
//
LOCUS       DAOTKU010000395         1613 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_61786_length_1613_cov_2.108472, whole genome shotgun sequence.
ACCESSION   DAOTKU010000395 DAOTKU010000000
VERSION     DAOTKU010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61786_length_1613_cov_2.108472"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="P5310_08130"
     CDS             <1..537
                     /locus_tag="P5310_08130"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HRU33362.1"
                     /translation="HGHHDGATEPAIVELSDGRILMFIRTNLDRFWQAISEDGGRYWR
                     TIMPSQIDASSSPGYLLKLHSGRLVLVWNRLQPEEGTYERPYNPQHSEVLASWFREEL
                     SIAFSEDDGKTWTKPVVIARQPGGQLSYPYIFEGQPGEIWIAAGFAFKRGWKDSLPFR
                     VKIDEERFINYLRRSEYA"
     gene            530..1573
                     /locus_tag="P5310_08135"
     CDS             530..1573
                     /locus_tag="P5310_08135"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRU33363.1"
                     /translation="MLNWGVIGCGGIAYRRTIPEGILRAKNARLLAVMDADKNKAKEV
                     GEKFGVRYYSNIEDILRDGDIQVVYIATPVYLHKEQIIHSAMAGKHILSEKPLALKSG
                     EAGEIIDVCKREGVKLGVGYMMRFHSLHEKAKEMIGNDELGKIVFARAQLSCWYPPIP
                     GAWRQDSALSGGGALIDMGIHSLDLLEYITGSEIEEVACFTDNVIHSYKSEDSSLVMV
                     RFKNKAHGVVDSYFNIPDNSSKNRLEIYGTKGSLLAEGTIGQSSSGEMFAYLEEDSKG
                     YNASQVRDIKGGMKIKVPLFNMYQAEVEAFTGSIEENKIPPVPGEVGLWSLKVIEACY
                     ESSRDKKFVRVGK"
BASE COUNT          535 a          242 c          422 g          414 t
ORIGIN      
        1 catggacatc atgatggggc aacagagcca gctattgttg aattgagtga cggaagaatt
       61 ctcatgttta taagaactaa cctggaccga ttctggcaag ctatatctga agatggtggc
      121 agatactgga ggactataat gccaagtcag atagatgcca gtagttctcc aggttatcta
      181 ttgaagcttc atagtggacg tttagtactg gtttggaata gacttcaacc agaggaaggt
      241 acttatgaaa gaccttataa ccctcagcat tctgaagtct tagcgtcctg gtttagagaa
      301 gagttatcta tcgcattttc agaagatgat ggtaagacct ggacaaaacc ggttgttatt
      361 gctcgtcagc caggaggaca gctgagttat ccatatattt tcgagggaca acctggagag
      421 atatggatag cagcaggttt tgcatttaaa agaggctgga aagattcttt gcctttccgg
      481 gtaaagattg acgaagaaag attcattaat tatctcagga ggtcagaata tgcttaactg
      541 gggtgttatt ggatgcggag ggatagctta taggagaaca atccctgagg ggatactgag
      601 ggctaaaaat gcaagactct tagcggttat ggatgcagat aagaataagg caaaagaggt
      661 gggggaaaag tttggagtaa gatattattc aaacatagaa gatatcctta gggatggaga
      721 tatacaggta gtttatatag ctacccctgt ttatctccat aaagaacaga ttatacattc
      781 agccatggca ggcaaacata tactatctga aaaaccttta gccttgaaat caggagaggc
      841 aggagagatt atagatgtgt gtaaaagaga aggggtgaaa cttggagtgg gctatatgat
      901 gagatttcat tctctccacg agaaggcaaa agagatgata ggaaatgatg aattgggcaa
      961 gatagtattt gccagggcac agctttcctg ctggtaccca cctatacctg gtgcctggag
     1021 acaggattca gctcttagtg gtggaggggc attaatagat atggggatcc atagtctaga
     1081 cctcctagaa tacataacag gatcagagat agaagaagtt gcctgcttta ccgataatgt
     1141 tattcacagt tacaaatcag aagattccag tctggtaatg gtgaggttta agaataaagc
     1201 ccacggagta gttgatagtt acttcaacat acctgacaat tcaagtaaga atagactgga
     1261 gatatatgga acaaagggta gtcttctggc tgaagggacc ataggtcaat cttccagtgg
     1321 agagatgttt gcctatttgg aggaggattc aaaagggtat aatgcctcac aggtaaggga
     1381 tataaaggga ggaatgaaga taaaagtccc tctttttaat atgtatcagg cagaggtaga
     1441 agctttcaca ggcagtatag aggagaataa gatacctcca gtacccgggg aggtgggatt
     1501 atggagtcta aaggtcatag aagcctgtta tgagtcaagc agagataaaa aatttgtaag
     1561 agtagggaaa taaattaata taaaaggagt gaacagaatg agcaaagaga aat
//
LOCUS       DAOTKU010000396         1605 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_62173_length_1605_cov_2.881290, whole genome shotgun sequence.
ACCESSION   DAOTKU010000396 DAOTKU010000000
VERSION     DAOTKU010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62173_length_1605_cov_2.881290"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(62..1039)
                     /locus_tag="P5310_08140"
     CDS             complement(62..1039)
                     /locus_tag="P5310_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014452629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRU33364.1"
                     /translation="MIGVIGATGHLGNVLTKELIKRGFKVRAIIPQGENVAHIKDDQV
                     EVYRADITDLSVITAAIAGLEYVFHCAGIINISSGNKDLMYRVNVEGTKNVIKACISN
                     NIKRLIYTSSVHAIAAPDKGIIDESREINPDKVYGFYAKTKALATLAMLESARSDLDA
                     VILCPSGIIGPFDYKTSEIGQLILDFVNRRLKAYIDGAYNFVDVRDVAAGHINAMEKA
                     RRGEIYILGGETISVFDILTLLESITGIKKPKIKIPYCIAYAISPFTPIYYSIVKTKP
                     LFTTYSIKVLRSNANISSKKAITELNYSFRPVRETIRDAYCWFKEQGKI"
     gene            complement(1057..1293)
                     /locus_tag="P5310_08145"
     CDS             complement(1057..1293)
                     /locus_tag="P5310_08145"
                     /inference="COORDINATES: protein motif:HMM:NF036901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU33365.1"
                     /translation="MYTAGDLVYYRFDAERRSFICEWREETDSDTVIYLPGISKDIKL
                     QPSYRFSTERVDQSDAEYVMIPSDFRNKRIEISL"
BASE COUNT          459 a          343 c          246 g          557 t
ORIGIN      
        1 aaacattata ggttataatt tttaggttgt caagggcgcg acgttcttca aaattctacc
       61 ttcatatttt accctgctct ttaaaccagc agtatgcatc tctgatagtt tctcttacag
      121 gtctaaaaga gtaatttaac tcagttatag cctttttact ggatatattg gcatttgaac
      181 gcaggacctt aatcgaatag gtggtgaaaa gaggtttcgt cttaactata gagtaatata
      241 ttggggtaaa gggggatatt gcataagcta tacagtatgg tatctttatt tttggttttt
      301 ttattcctgt tatagactca agaagagtta aaatatcaaa tacagaaata gtttctcctc
      361 ctaaaatgta tatctctcct cttctagcct tttccatagc gttaatgtgt ccagccgcta
      421 catctctaac atcgacaaaa ttatatgctc catcaatata agcttttaac cttctattta
      481 caaaatccag aatcaactgc ccaatctcag acgtcttata atcaaaagga cctataattc
      541 ctgaagggca tagaatgaca gcgtcaagat cagatcttgc cgattctaac atggcaagtg
      601 tagctaatgc ctttgtcttt gcgtaaaagc catagacttt atctgggttg atttcacggg
      661 attcatcaat tattccttta tcaggagcag ctatagcgtg gacagagctg gtatatataa
      721 gtcgtttaat attattactg atacaggctt taataacatt ttttgttcct tccacattta
      781 ctctgtacat caggtcttta ttcccgcttg agatattaat aatgccagca cagtggaaga
      841 catattcaag tcctgcgata gcggcagtga tgacggacag atcagtaata tctgccctgt
      901 atacctcaac ctggtcatcc tttatatggg ctacattttc tccttgaggt ataattgctc
      961 ttaccttgaa acctctcttt atgagctctt ttgtaaggac attccctaaa tgtcctgttg
     1021 cccctattac tcctatcatc ccctgactct ccaatcttaa agggatatct ctattctttt
     1081 atttctgaaa tcacttggaa tcattacata ttcagcatca gattgatcta ctctctctgt
     1141 agaaaatctg tatgatggtt gtaatttaat atctttagaa atccctggta aatatataac
     1201 tgtatcacta tcagtctctt ctctccattc acagataaaa cttcttctct ctgcatcaaa
     1261 cctgtaataa actaaatccc cagctgtata cacaggatat ggtcgtctga tagcatctat
     1321 atattcacta tcttccagtt ctctataacc agaataagcc tagtacgttt cactacattt
     1381 tagttcttcc atagctttca ctataaaaag cgcaatctcc tttacatctt tagaccttcc
     1441 aaatgctccc cattctccaa taagcattgg aatccccaac ctgttctggg tgtctttatg
     1501 ccttctaaga atcaccgaga tagtgaatta tatgatctgg tcttttctag gaatttagtc
     1561 ttcatcgctt cccatacctt atctatgtca gaacctataa aaggc
//
LOCUS       DAOTKU010000397         1594 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_62773_length_1594_cov_3.437297, whole genome shotgun sequence.
ACCESSION   DAOTKU010000397 DAOTKU010000000
VERSION     DAOTKU010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62773_length_1594_cov_3.437297"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="P5310_08150"
     CDS             <1..375
                     /locus_tag="P5310_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HRU33366.1"
                     /translation="DEYIELHGPVPVGEATITSGGKLKARYIIHTVGPRWGEGDEEEK
                     LRKAIKNALELASERGIRSISIPAVSCGIFGFPQEKGTKIIVDTIINYIKEKKNPFIE
                     IHLIGLGRDVTELFKKHLEGKE"
     gene            372..803
                     /locus_tag="P5310_08155"
     CDS             372..803
                     /locus_tag="P5310_08155"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HRU33367.1"
                     /translation="MNYTIVKMDSRKSIALIAHDNKKGELLEWAKNNREILIQHKLYA
                     TATTGRLLEEKLGLNITKFKSGPLGGDQQIGAMIADGKIDMLVFFWDPLEPLPHDPDI
                     KALLRIATVWNIPIATNLSSADFIISSPFIPGEYKRSVPEI"
     gene            800..>1594
                     /locus_tag="P5310_08160"
     CDS             800..>1594
                     /locus_tag="P5310_08160"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HRU33368.1"
                     /translation="MRRRLSLISTIIISIILGFIILGSVTGYVIFFRSNKHSLLQAYE
                     YLDSITRLISQDVSSLLEEKIRLLTLTREEFFDGSISENLLENLSNLFDCGIMYIDEN
                     GIVKKAYSRYEFELMGKDVSNREYFIQTRKTLKPYVSEGFKAITGRDVMVITVPVIKN
                     NSFKGLIAGCTDLRNQKIGYLISSSRFYKTGRITVIDYKGTVLFSEDPTEIGKIFTQF
                     PLYRAGHREIKINGKDYIVEVKSIPDTRWMIVASIEKEDILNYSYQN"
BASE COUNT          575 a          249 c          359 g          411 t
ORIGIN      
        1 gacgaatata tagaactaca tggaccagtt ccagtaggag aggctactat tacctcaggt
       61 ggaaaactaa aagccaggta tattatacat acggtaggtc caagatgggg agagggggat
      121 gaggaagaaa aactccggaa agcgataaaa aacgctctgg aactggcatc tgaaagagga
      181 atcaggagta taagtatacc ggcagtgagc tgtgggatat ttgggtttcc tcaggaaaag
      241 gggacaaaga tcattgttga tacaataata aattatatta aagagaaaaa gaatcctttt
      301 atagagatac atcttatagg actgggaaga gatgtcacag aactatttaa aaaacatcta
      361 gagggaaaag aatgaactat acaatagtta agatggatag tagaaaaagt atagccctga
      421 ttgctcatga taacaagaag ggagagctcc tggaatgggc aaaaaataat agagagatat
      481 taatccagca taaactttac gctacagcca caacgggaag gttactggaa gagaaattgg
      541 ggctcaatat tacaaagttt aaaagtggac ccctaggtgg agaccagcag ataggagcaa
      601 tgattgctga tggaaagatt gatatgcttg tgtttttctg ggacccactt gagccacttc
      661 cccacgaccc agacataaaa gccctgctaa ggatagcaac agtatggaat ataccaatag
      721 ccaccaatct ttcatctgca gactttatca tctcttctcc atttatacca ggggagtata
      781 aaagatctgt accggagata tgagaagaag actatctctg ataagcacca taattatcag
      841 tataattctc ggttttatta ttcttgggtc tgtaaccggt tatgtaatct tcttcagaag
      901 taacaaacat agtttactcc aggcttatga gtatctcgac agtataacca gactgatatc
      961 tcaagatgtg agttctctac tggaagaaaa gatcagatta ttaacactga caagggaaga
     1021 gtttttcgat ggaagtataa gcgagaatct gctggaaaac ctctccaatt tatttgactg
     1081 tggtattatg tatatcgatg aaaatggaat cgttaaaaaa gcctattcca gatatgaatt
     1141 tgaactcatg gggaaagatg ttagtaacag ggaatatttc atacagacca ggaagacttt
     1201 aaagccgtat gtgagcgaag ggtttaaggc tatcactggt agagatgtga tggttataac
     1261 cgttccggta attaaaaata atagttttaa ggggttgata gcaggatgta cagatttaag
     1321 aaatcaaaag attggttatc ttatcagctc cagcaggttt tataaaacag gaaggatcac
     1381 agtaatagat tataagggta cggttctttt cagtgaagac cccactgaga taggaaagat
     1441 atttacccaa ttccccctgt acagggctgg acatagagag ataaaaatca atggtaaaga
     1501 ttacatagtt gaagtgaaat caatccccga tacaagatgg atgatagtgg caagtataga
     1561 aaaagaggat atactcaact attcttacca gaat
//
LOCUS       DAOTKU010000398         1582 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_63431_length_1582_cov_3.742633, whole genome shotgun sequence.
ACCESSION   DAOTKU010000398 DAOTKU010000000
VERSION     DAOTKU010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63431_length_1582_cov_3.742633"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..705)
                     /locus_tag="P5310_08165"
     CDS             complement(<1..705)
                     /locus_tag="P5310_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU33369.1"
                     /translation="MKKLVIGFLLVSMILVPMFVSAQQGDTAVENRMMVSSLLSAFPD
                     FLTEDIINQLRAQGYGYGEIAIISVIATQSSNNITDVINYAKENNLGWGETANYFGVK
                     LSRIGLYINPKDEAEISGMNYLLRQRYQLSNYDIYRLRLKGLKSDEILVCYELYSRLD
                     PKGENPELLQSIIRAREQKRDWNRIVEQVSNAGQSAQIREEAKARGKNQQQGNQQQGN
                     QQQGNQQQGNQQQGNQQ"
     gene            1075..>1582
                     /locus_tag="P5310_08170"
     CDS             1075..>1582
                     /locus_tag="P5310_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU33370.1"
                     /translation="MCIIAVCIDRKLSLEEIDYGWTRNPDGGGIGWVENGRVNYIKGL
                     MTLEDFKKEYENLNILPHIVHFRNASKGIPVNCKLTHPFIVSKTSPIRLRYKGIKPVL
                     FHNGTIRDWKRYYEQYVESRGIIIRDENLSDTRVMAIIWAEKGEDTFDLLNPGRVALL
                     YPDGKIKKY"
BASE COUNT          468 a          294 c          300 g          520 t
ORIGIN      
        1 ctgttggttc ccttgctgct ggtttccctg ctgttggttc ccttgctgct ggtttccctg
       61 ctgttggttc ccttgctgct ggttttttcc tctggctttt gcctcttctc taatctgagc
      121 tgactgacct gcattactta cctgttccac tatcctattc caatctctct tctgttctct
      181 agccctgata atactctgta agagttcagg attttcccct tttgggtcta atctggagta
      241 taattcataa cataccagga tttcatctga ttttagtcct ttcagtctga gcctatagat
      301 atcatagtta gagagttgat atctttgacg aagcaaatag ttcataccag aaatttctgc
      361 ctcatctttt gggtttatat ataacccaat cctggatagc ttaactccaa aataatttgc
      421 agtctctccc cagcctaaat tattctcctt agcatagttt atcacatctg ttatattatt
      481 actactctga gttgctatga cagatattat cgctatttca ccgtatccat agccctgggc
      541 acgtagttga ttgataatat cttctgttaa aaaatctggg aaggcagata aaagactact
      601 caccatcatc ctgttctcca cagcagtatc cccttgctgt gcagatacaa acataggtac
      661 taaaatcata ctaaccagca gaaaaccaat cactaacttt ttcataatat cacctccatt
      721 caaaattttt atagcttcta ctctagagta tatattcaaa aaacataatt gtatgtgacg
      781 taaatcacaa taaaattttt cttatgctaa atgctgttaa ccgttttata cttctgtgag
      841 tcaggagtat ttccctctcc tagaaggagt aatgcataat gcatcatgaa taggggttgt
      901 tttacttctc tctccttgat gggagaatgc atgggtgagg tttgcctcct tgaaagagaa
      961 gagaatagaa cgggttattc ctgaatttgt atttatacta tcagtaagta ttataatctt
     1021 attagatagc attttcctgg actataaata gaaaaaccag caggaggcgc ttggatgtgt
     1081 ataatagcgg tatgcattga taggaagctc tctttggaag agatagatta tggatggaca
     1141 aggaatccag acggaggtgg aattggctgg gtagaaaatg gcagggtgaa ttatataaag
     1201 gggttaatga ctcttgaaga ttttaaaaaa gagtatgaaa atctaaatat tcttccccat
     1261 atagttcatt tccgaaacgc atcaaaaggt atccctgtca attgtaaact tacccatccg
     1321 ttcatagtga gtaaaacatc gccgataaga ctgagatata aggggataaa gcctgtgctt
     1381 tttcataatg gaactattag ggattggaaa agatattatg agcagtatgt agaatcaaga
     1441 ggaattataa ttcgagatga gaacctttca gatacaaggg ttatggcaat catatgggca
     1501 gaaaaaggtg aagatacttt tgatttactt aacccgggga gagtcgctct gttatatcca
     1561 gatggaaaga taaaaaaata tg
//
LOCUS       DAOTKU010000399         1577 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_63744_length_1577_cov_2.659658, whole genome shotgun sequence.
ACCESSION   DAOTKU010000399 DAOTKU010000000
VERSION     DAOTKU010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63744_length_1577_cov_2.659658"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /gene="leuB"
                     /locus_tag="P5310_08175"
     CDS             complement(<1..823)
                     /gene="leuB"
                     /locus_tag="P5310_08175"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012994198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HRU33371.1"
                     /translation="MFKIAVLPGDGIGPEVVEEGVKLLRALDRELNIGFVFETALIGG
                     IAVDNYGIPFPDDTLKTVLNSDMVLLGAVGGPKWDNIEVSKRPEQALLALRKNLGVFA
                     NIRPVKSIKPLISASPIKPEIIDGVDLIILRELTGGIYFGEPKYRDREKAIDTLVYNR
                     YEIERIARVAFKMSLSRRKKITSVDKANILESSRLWREVVNEMSTDYPDVMIDHLYVD
                     NCAMQLVRRPKDFDIILTENMFGDILSDEAGAILGSLGMLPSASLGDKYALYEPIH"
     gene            complement(816..1310)
                     /locus_tag="P5310_08180"
     CDS             complement(816..1310)
                     /locus_tag="P5310_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HRU33372.1"
                     /translation="MTGKVWKFGDNIDTDIIIPARYLVTQDIEELGKHCMEGVMPGFS
                     SMVSKGDIIVAGRNFGCGSSREHAPLAILGAGISAVIAISFARIFFRNSINVGLPVLI
                     SKEAVEESGEGDILEVNIESGKIYNKTTGKWYNSEPYPPQLVEIIKSGGLTSYTKRRL
                     GINV"
BASE COUNT          450 a          384 c          251 g          492 t
ORIGIN      
        1 catggatagg ttcatacaga gcatatttat ctcccaggct agcggaagga agcatcccca
       61 gggaacccag aatagccccc gcctcatcac tcaatatatc accaaacata ttctctgtta
      121 atattatatc aaaatccttt ggtcttctta ctagttgcat cgcacagtta tctacataga
      181 gatgatctat cataacatcc ggataatccg tagacatctc attgacaacc tctctccaga
      241 gtctagaact ctccaggata tttgccttat cgacggatgt gatcttcttc ctacgtgata
      301 atgacatctt aaaagcaacc ctggctatcc tctctatctc atacctgtta tagacaagtg
      361 tatctatagc cttctctcta tccctgtatt ttggctctcc aaagtatatc cctcctgtaa
      421 gctctctcag tatgatcagg tctaccccat ctattatctc gggttttata ggggaggctg
      481 agattagagg tttgatagac tttacaggtc ttatattggc aaaaacacca aggtttttcc
      541 tcaaagccaa cagcgcctgt tctggtcttt ttgagacctc tatattatcc cattttggtc
      601 ctccaacagc tccaaggagt accatatctg aattcagtac tgttttcaat gtgtcatcag
      661 gaaatggtat gccataatta tctaccgcta taccaccaat aagagcagtc tcaaagacaa
      721 aaccaatatt taactctcta tccagagccc tgagtaactt taccccctct tcaaccactt
      781 ctggacctat accatctccg gggagaacag ctatcttaaa cattgatccc cagtctcctt
      841 ttagtatagc ttgtgagtcc accacttttt ataatctcta caagttgtgg cggatatggc
      901 tctgagttat accattttcc ggtagtctta ttgtatatct tcccggattc gatgtttacc
      961 tccagtatgt caccttcacc ggattcctca accgcctctt tagaaataag aacaggtaat
     1021 cccacattga tactgtttct aaaaaatatc ctggcaaaac ttatagctat aactgcactt
     1081 attccggcac ccagtatggc aagtggggca tgttctctag aacttccaca gccaaaattt
     1141 ctcccggcaa ctatgatatc tcctttggag accatagagg aaaatcccgg cattaccccc
     1201 tccatacagt gcttacccag ctcttctata tcctgggtca caagatacct ggcaggtata
     1261 ataatatcgg tatctatatt gtcaccaaac ttccatactt ttccagtcat atttttaaac
     1321 ctccaaacat tatactgttg tgaattttaa ccttaaaaga tattcaagtc aagaaaaaga
     1381 tgatataatt atataatgga ggtgaaactt atgctaaaaa ttttgccatt tattcttata
     1441 tttttgctcc cgacgggagc atacgggaaa gagaaagaga ttgaaataac ataccttggt
     1501 cattcgatgt tcttgataaa atcagatact gtatctatag ttaccgatcc atttgaccct
     1561 tcaaccggat ttcctgc
//
LOCUS       DAOTKU010000400         1574 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_63907_length_1574_cov_2.868993, whole genome shotgun sequence.
ACCESSION   DAOTKU010000400 DAOTKU010000000
VERSION     DAOTKU010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63907_length_1574_cov_2.868993"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1574
                     /locus_tag="P5310_08185"
     CDS             <1..>1574
                     /locus_tag="P5310_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HRU33373.1"
                     /translation="EYIHIGSEDIPFARSYTIPYSALFRKTFKAEKQIKRARVYVSGL
                     GFYELYINGKRIGDKVLDPGQTDYKKRVLYSIYDVTDELNTGANAIGVMLGNGRYVKD
                     YGYDFPRMILQLEIEYLDGTTEVVVSDDTWKTSYGPIKANGIYYGEDYDARREIPSWN
                     LSDFNDVNWEKVKLVDPPGGRLVAELMPPVKVIKTLKPQKIISPKPGVYVYDFGQNFT
                     GWVRLYVNGPEGTEIKLRFAELIYDDGNINLTPTRGARATDIYITRGDGLEIYEPRFT
                     YHGFRYVELTGFPGVPTLDTLEGCVVYSSVRQVGEFNCSNEVINKIHGNILWGQLSNL
                     MSIPTDCPQRDERMGWMGDAQLTVEEAIYNFDMASFYIKYLDDIKDAQKENGSVSDVV
                     PPYWSIYPADPAWGTAYITIAWYLYQYYGDTEVLKDHYEGLKKYVDFLTGLAPDNILR
                     FSKYGDWCPPGSIRAKDLSGELVSTWCYYNDTLTLSRIADILGKREDHTKYSKLAEDI
                     KAAFNKQYLKERSYAV"
BASE COUNT          477 a          261 c          395 g          441 t
ORIGIN      
        1 tgagtatatc catatcggtt cagaggatat tccgtttgct agaagttata caatccctta
       61 ctctgcacta tttagaaaga cattcaaggc agaaaaacag ataaagaggg cgagggttta
      121 cgtttctggt cttggatttt atgagctcta tataaacggt aaacgtatcg gagataaggt
      181 cctcgatcct gggcagacag attataaaaa gagagtttta tactctatat atgacgttac
      241 agatgaattg aacaccggcg ctaatgccat tggtgttatg ctggggaatg gaagatatgt
      301 taaagattac gggtatgatt ttcccaggat gatcttgcaa cttgagatag aatatctgga
      361 tggaactact gaagtggttg tcagtgatga tacctggaag acaagttatg gaccaataaa
      421 ggcaaatgga atatattacg gtgaagatta tgatgcaagg agagagatac catcctggaa
      481 tctgtcagat tttaatgatg ttaactggga aaaggtaaag ctggtcgacc ctccgggggg
      541 aagattggta gctgagctta tgccccctgt aaaggttatt aagacgttga agccgcaaaa
      601 gattataagt ccaaagccag gggtatacgt atatgatttt gggcagaact tcaccggttg
      661 ggtaagatta tatgtgaatg gtccggaagg gacagagata aagcttagat ttgccgagct
      721 tatatatgac gatgggaata taaatcttac acctaccaga ggggcaaggg ctactgatat
      781 ctacatcacc aggggtgatg gcttggagat atatgagcca agatttacat atcacggctt
      841 ccgttatgtg gagcttaccg gatttccagg tgttcctacc cttgataccc ttgaaggttg
      901 tgttgtctat tcctctgtac gtcaggtggg agagtttaat tgttccaacg aggtgattaa
      961 taagatacac gggaatatcc tgtggggtca gttgagtaac cttatgagca taccgacaga
     1021 ctgtccgcag agggatgaga ggatgggatg gatgggagat gctcaactta cagtagaaga
     1081 ggctatttat aattttgata tggctagctt ttatataaaa tatcttgatg atataaaaga
     1141 tgctcagaag gagaatggga gtgtttctga tgttgtcccg ccatactgga gtatctatcc
     1201 agcagaccct gcctggggaa ccgcctatat cactatagcc tggtatctct accagtacta
     1261 cggggatacc gaggtattga aggaccacta tgaagggtta aagaaatatg tagatttctt
     1321 aactggactt gcacctgata atattttgag attctccaag tatggagact ggtgtcctcc
     1381 aggaagcata agggctaaag acctctctgg agaacttgtt tctacctggt gctattacaa
     1441 tgatacattg accctctcca ggatagcaga tattctgggt aaaagagaag atcatactaa
     1501 atattctaag ctggcagaag acataaaggc tgcatttaat aagcaatatt taaaagagag
     1561 gtcctatgct gttt
//
LOCUS       DAOTKU010000401         1573 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_63962_length_1573_cov_3.006588, whole genome shotgun sequence.
ACCESSION   DAOTKU010000401 DAOTKU010000000
VERSION     DAOTKU010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63962_length_1573_cov_3.006588"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(96..785)
                     /locus_tag="P5310_08190"
     CDS             complement(96..785)
                     /locus_tag="P5310_08190"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRU33374.1"
                     /translation="MMGDNKISILKEIPLFAGLGEDLLLKIEAISIEKSYKKGEIIIL
                     QDTSVEGLYILVSGRLRISRSSEDGRVKVLAILSPGDIIGEMSLLDDESASATVETME
                     DSRLILIRKEDFQAMLLRYPLVTIEIARILGRRLRSADKEIEELAFYSVKNRLIEALI
                     DLANRHSERTSSGIRVSLRITHQDLADMVGSSRETITRIMNLLERDQLIVNEGGYIVI
                     KDINRLKTYLL"
     gene            complement(772..>1573)
                     /locus_tag="P5310_08195"
     CDS             complement(772..>1573)
                     /locus_tag="P5310_08195"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRU33375.1"
                     /translation="TNPIRGSIAGLIGVFICILGVFLLFLNGIIFDIAPTILSILSTI
                     VSSDISSYIKEREEKDKIREIFQMYLSPEVVNKLLDHPELWGLKGEETEVTVMFVDIS
                     GFTKFSQTHKPQEVVEFLNRYFSIVTDIIFKYGGTLDKFIGDAVMAIYGAPVRYEDHR
                     LKAIESALEIIDISRKNGIRVKIGINTGKVILGNIGTDKRVQYTAIGDAVNIAEFLES
                     IGEPNEIIIGEETYRSIKDKVRAKEIILEGKYKGVRAYKIIEDKDDGG"
BASE COUNT          447 a          353 c          227 g          546 t
ORIGIN      
        1 aaaataatca aggtcaggag tttcagtatg gacaaaataa aagggattct aatggagaag
       61 gacctaatcg tccttcgaat cgctaggacc cggggttaga gaaggtaagt tttgagcctg
      121 tttatgtcct ttataacaat ataaccaccc tcattaacta tcagctggtc tctctccaag
      181 agattcatta tcctagttat cgtttcccta gatgaaccta ccatatctgc taaatcctga
      241 tgggtaatcc tcaatgagac ccttatacct gaagaagtcc tttcactatg cctgttcgca
      301 agatctatta atgcctcaat aagcctattt tttacactat agaaggcaag ctcttcaatc
      361 tctttatctg cagatctaag tcttctacct aatattctgg ctatctctat ggtgacaaga
      421 ggatatctaa gaagcatcgc ttgaaaatcc tctttcctta taagtatcaa tctggaatct
      481 tccatcgttt ctacagttgc agaggcagat tcgtcatcta atagagacat ctcgcctatg
      541 atatccccag gtgaaagtat agctaagacc tttactcttc catcctcaga acttctgctt
      601 atcctgagtc tacccgatac caggatatag agtccctcta ctgaagtatc ctgcaaaatt
      661 atgatttctc cctttttata tgacttttct atagagatag cttctatctt cagaagcaga
      721 tcctctccaa gtccagcaaa tagaggtatc tctttaagaa tacttatctt attatccccc
      781 atcatcttta tcctctatta tcttatatgc ccttaccccc ttatactttc cttctaagat
      841 tatctcttta gcccttacct tatcctttat gcttctatat gtctcttctc ctataataat
      901 ttcattgggt tctcctatag attccaggaa ttctgcgata tttaccgcat ctcctatggc
      961 agtgtattgt acccttttat ctgttcctat attcccaagt ataaccttgc cagtatttat
     1021 cccaatctta acccttatgc cattcttcct agatatatct ataatttcaa gagcagactc
     1081 tatagccttt aatcgatggt cctcatatct gactggagct ccgtatatcg ccatcactgc
     1141 gtctcctata aatttatcta aagtccctcc atacttgaag atgatatcag ttacaataga
     1201 gaagtatcta tttaaaaatt ctactacctc ttgaggttta tgggtctgag aaaatttagt
     1261 aaatccgctt atgtctacaa acattacagt tacttctgtt tcttcacctt ttaaacccca
     1321 tagttcagga tgatctaaca gtttgtttac cacttctgga gataagtaca tttgaaatat
     1381 ctctcttatc ttatcctttt cttctctctc ttttatatag gatgatatat cagaagatac
     1441 tattgtagat agtatagata aaattgtggg ggctatatca aatattatcc catttaaaaa
     1501 gaggagaaat acacccagga tacatataaa tactccaatt aagccagcta ttgatcccct
     1561 gattgggtta gtg
//
LOCUS       DAOTKU010000402         1569 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_64168_length_1569_cov_2.884412, whole genome shotgun sequence.
ACCESSION   DAOTKU010000402 DAOTKU010000000
VERSION     DAOTKU010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64168_length_1569_cov_2.884412"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /gene="hisF"
                     /locus_tag="P5310_08200"
     CDS             <1..629
                     /gene="hisF"
                     /locus_tag="P5310_08200"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006569519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HRU33376.1"
                     /translation="ELVFLDITASIEKRKTIVDLVMKTASRVFIPFTVGGGIRSVEEI
                     REILRYGADKVSINTQAVMNPSLIEESAEIFGSQCIVVAIDAKRDPSIDGWRVYINGG
                     TTPTELEAVGWAKEVERLGAGEILLTSIDTDGTQGGYDIELTRAVVEAVNIPVIASGG
                     AGSLEHIRDVLKDGKASAALIASLLHYKKYTIREIKEYLKKEGIPVRL"
     gene            638..1243
                     /gene="hisIE"
                     /locus_tag="P5310_08205"
     CDS             638..1243
                     /gene="hisIE"
                     /locus_tag="P5310_08205"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HRU33377.1"
                     /translation="MVKFDKEGLIPAIIQEASSGKVLMLGYMNEESLKKTVETGTTWF
                     WSRERQKLWNKGETSGNIQKVKEIRLDCDGDALLILVAQTGVACHTGNLSCFFQNFYT
                     KEPASTFNLETLYDIILSRKLNPVDGSYTCKLFKEGVERISKKVGEESVEVILAGIKG
                     DRDEVIYEVSDLFYHTLVLLASLDISPQEIYSELNRRHKKE"
     gene            1278..>1569
                     /locus_tag="P5310_08210"
     CDS             1278..>1569
                     /locus_tag="P5310_08210"
                     /inference="COORDINATES: protein motif:HMM:NF015019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HRU33378.1"
                     /translation="MNNTWLVLSTIIGGGTLLLAYYFYTWVLKQPAGTEKMKEISGAI
                     HEGAVAFLKREYLTLVYFIIIMTAIIAIFINPYSAIAYVMGSALSIFAGWFGM"
BASE COUNT          515 a          253 c          400 g          401 t
ORIGIN      
        1 acgaactggt atttctggat ataacagcat ctatagagaa gagaaagacc atcgtagacc
       61 tcgtaatgaa gactgcatcc agagtattta taccatttac tgtaggaggt ggtataagaa
      121 gtgtagaaga gataagggaa atattaagat atggggcaga taaggtatcc ataaatacac
      181 aggcggtgat gaatccatct ctgatagaag agtcagcaga gatattcggg tctcaatgta
      241 tagttgtagc tatagatgca aagagagacc cttctataga tgggtggagg gtatatataa
      301 atggggggac aactcccact gagcttgagg ctgtgggctg ggctaaagag gtggagagat
      361 tgggagcagg agagattctg cttacaagta tagataccga tgggacacag gggggatatg
      421 atatcgagct taccagagcg gtggttgaag cggtcaatat cccggttata gcatctggag
      481 gggctggaag cttagaacat ataagagatg tgttgaaaga tggaaaagca agcgccgctc
      541 tgattgcctc actgctccac tataagaagt atacgataag agagataaaa gaatatttaa
      601 agaaggaagg gatcccggta cgtctttaga tatatctatg gtaaagtttg ataaagaagg
      661 tttgatacct gctatcatcc aggaagccag ctcaggtaaa gtactaatgc ttggttatat
      721 gaatgaggaa tctttaaaga aaactgtgga aactggaact acctggttct ggagcagaga
      781 gagacagaag ctatggaaca agggggaaac cagcggtaat atacaaaagg taaaagagat
      841 aagactagac tgtgatggag atgccctgct gatactggtg gcacagacag gggtagcctg
      901 tcataccggt aatctgtcct gtttctttca gaatttttat acaaaagaac ccgcctctac
      961 atttaatctt gagaccctct atgatatcat tctttccaga aaacttaacc ctgtggatgg
     1021 ttcttacacc tgcaagcttt ttaaagaagg agtggagagg atatcaaaaa aggtaggaga
     1081 ggaatcggta gaggtcatac ttgcaggtat aaaaggggac agggatgagg tgatatatga
     1141 ggtctcagat ctcttctatc acactttagt tttgctagca tctctagata tatctcctca
     1201 agaaatatac agtgagttaa acaggaggca taaaaaagaa tgaaccacaa aattctaaag
     1261 gagggagtga agtctctatg aacaacacct ggctagtgct ttccacaatt ataggaggtg
     1321 ggacattatt actggcgtac tacttctata cctgggtgtt aaaacagcca gcaggaacag
     1381 agaagatgaa agagatatct ggtgctatcc atgagggagc ggtagccttt ctaaaaagag
     1441 agtatttaac tcttgtatac tttattataa taatgactgc cataatagca atattcatca
     1501 atccctacag cgctatagcc tatgtgatgg gtagcgcatt atctatcttt gctggatggt
     1561 ttggcatgc
//
LOCUS       DAOTKU010000403         1565 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_64388_length_1565_cov_4.528477, whole genome shotgun sequence.
ACCESSION   DAOTKU010000403 DAOTKU010000000
VERSION     DAOTKU010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64388_length_1565_cov_4.528477"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="P5310_08215"
     CDS             complement(<1..267)
                     /locus_tag="P5310_08215"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRU33379.1"
                     /translation="MQLMKDFFQVGGSLNGITWTGNDASYDDCNTYILKTDEGLIMFD
                     CGCGDTLEQIFNNMLYWDLNPEDIKACFITHPHLDHAGACHLLAD"
     gene            complement(422..1492)
                     /locus_tag="P5310_08220"
     CDS             complement(422..1492)
                     /locus_tag="P5310_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU33380.1"
                     /translation="MELKYKPDFEEAKRYWRAFWDKEIIDRPCVIITSPKNGLIQSPH
                     PPYMAGSDGNYKEALEMYESWASTIYFGAEAIPFFEISLGPDEIAASLQGAGELVKDE
                     ETKTSWLNPFVKDWNSFPEIKIDEESAAWKRIIDFYQYGATFSEGKFLLGGLDFHTNM
                     DLLRAIRGSDNLCIDLLDCPEEIEKRMKEARKIFPFVYEAMYKAGRMKERGTIGWIPL
                     YCEGRFAVIQCDFICMISPALSRKFVIPALEEEASYLDHCIYHLDGPGTFPHLDDILA
                     ISKIDAIQWVPGDGQPPHIRWMDLFKKILSRNKSVIIYPNSIEEIKIFHKELGPKGVV
                     YQIALPSEKEAEELITWLRIHT"
BASE COUNT          454 a          352 c          238 g          521 t
ORIGIN      
        1 atcagccagc agatggcagg cacctgcatg atccagatga gggtgtgtta taaagcaggc
       61 tttaatatcc tctggattta aatcccaata taacatatta ttaaatatct gttccagggt
      121 atcaccgcaa ccacagtcaa acattataag cccttcatct gttttaagga tatatgtatt
      181 gcagtcatca tagcttgcat cattccctgt ccaggtaata ccatttaggc ttccacctac
      241 ctgaaagaaa tccttcataa gttgcatatt ataccatcct cctgtaatag attattccag
      301 tatggatttt agctgttcga cagcttgctt tgtatctata gaaaccaatc tatgtgcatc
      361 tgtacccata tggaagatga ctcctacctc tctgcctcga cgttcctcaa aattaaatct
      421 tctaggtatg gattcttagc caagtaatta attcttctgc ttctttttct gaaggaagag
      481 caatctgata caccacacct tttggtccta attccttgtg gaatattttt atttcttcta
      541 ttgagttagg atatatgatc acacttttat ttctacttaa gattttctta aaaagatcca
      601 tccatcttat atgaggtggt tgcccatctc cagggaccca ctggatagca tcaatttttg
      661 atatagcaag aatgtcatct aaatgaggga atgtccctgg accatctaaa tggtagatgc
      721 aatgatctaa ataactagcc tcttcttcta aagcaggtat aacaaacttt ctggaaagag
      781 ccggacttat catgcaaata aaatcacact gaatcacggc aaaccttcct tcacaataaa
      841 gagggatcca acctattgtt cctctttctt tcattcttcc tgctttatac attgcttcat
      901 atacaaaggg gaatatcttt cttgcctctt tcatcctctt ttctatctcc tctggacaat
      961 cgagtaaatc tatacatagg ttgtctgatc ccctgatagc tcttaataaa tccatgttgg
     1021 tatgaaaatc tagacctcct aaaagaaact tcccttcgct aaacgttgct ccatactgat
     1081 aaaaatctat tattctcttc caagcagcac tttcttcatc tatctttatc tcggggaagc
     1141 tgttccaatc tttaacaaag ggattaagcc aggaagtttt tgtttcctca tcttttacta
     1201 actctccggc tccctgtaaa gaagcagcta tttcatcggg tccgagagag atttcaaaaa
     1261 agggaatcgc ctctgctcca aaatatatag tagatgccca actttcatac atctccaagg
     1321 cttctttata atttccatca gaaccagcca tataaggagg atggggtgat tgaattagtc
     1381 catttttagg agaagtaata atgacacagg gtctatcaat tatttcttta tcccaaaatg
     1441 ccctccaata tctctttgct tcctcaaagt caggtttata ctttagttcc ataatctgtc
     1501 tcctctctca gtttttctta attctaaata gcctatatcc atccctcaaa tataccaaaa
     1561 ggaga
//
LOCUS       DAOTKU010000404         1565 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_64404_length_1565_cov_2.869536, whole genome shotgun sequence.
ACCESSION   DAOTKU010000404 DAOTKU010000000
VERSION     DAOTKU010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64404_length_1565_cov_2.869536"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="P5310_08225"
     CDS             complement(<1..661)
                     /locus_tag="P5310_08225"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRU33381.1"
                     /translation="MHKFFAVILTLVLILSMTASAFSAPVELIYTYWGSPLERDAQAK
                     MCRDFEKAYQDIFPGIKIKDIYIPSNYDEKINAMVAAGEPPHVAQLGEALALEWAEKG
                     ILLDILPLIKEDKDPSVKIENRLSMTWYWYDKGKKTIGTNLAPEVMVLWYNKELFDKA
                     GVPYPTSDPNAWTWDKFLDTAIKLTVDRNGRHPGDQGFDPSNIDVYGVAFGTWWAAIL
                     PF"
     gene            complement(704..1408)
                     /locus_tag="P5310_08230"
     CDS             complement(704..1408)
                     /locus_tag="P5310_08230"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HRU33382.1"
                     /translation="MVRPVNRIKLTESIIEAIVEYASSSNLKIGDKLPSERELSNELR
                     VSRPLLREALRKMESLNLIEVLPGKGIFIKAPLKNDTSYLILHINQDKDKVLEILEIR
                     RVLEKYALEEAIKNIDDGEIGELERIVNLLEEKLRRGDDSREENWAFHSIIYGSSGNG
                     LLFDILDGFKEFHLFWEDPIKCPSFADKTYSFHRELFKGIKEKDIRRIHSVIDKLIDM
                     IEDEVERNGSVPRNGG"
BASE COUNT          480 a          364 c          235 g          486 t
ORIGIN      
        1 taaaaggaag tatcgcagcc caccaggtac caaaagcaac accataaaca tctatattgc
       61 tcggatcaaa accctgatca cccggatgtc taccgtttct atcaacagta agctttattg
      121 ctgtatcaag aaacttatcc caggtccagg catttggatc tgaagttgga tatggaactc
      181 ccgccttatc aaagagctct ttattgtacc agaggaccat tacttctggc gcaaggttag
      241 tacctatggt tttcttcccc ttatcatacc aataccaggt catagagagc ctattttcaa
      301 tcttgacaga aggatcctta tcctccttta tcaagggaag gatatccagt aaaataccct
      361 tctcagccca ctccagagca agagcttctc ccagttgtgc cacatgtgga ggctcaccag
      421 cagcaaccat ggcatttatc ttttcatcat agtttgatgg gatatagata tctttaatct
      481 tgatccctgg gaagatatcc tgatatgctt tctcaaaatc tctacacatc ttagcctgag
      541 catctctctc gagtggacta ccccagtatg tatagataag ttctactgga gcagaaaaag
      601 cagaagcagt catacttaaa atcagcacta gagttaaaat aactgcaaaa aacttatgca
      661 ttttcttcct cctcctttaa ttttgtagtt cttaaaacta atattatccc ccatttcttg
      721 gtacactacc attcctttcc acctcatcct caatcatatc tatcaactta tctattacag
      781 aatgtattct cctgatatcc ttctccttta tccccttaaa gagctccctg tgaaaggaat
      841 aagttttatc cgcaaaagaa gggcatttta tcggatcttc ccagaagaga tgaaactctt
      901 taaaaccatc caggatatca aaaaggagac cattcccact ggaaccataa attatactgt
      961 gaaaagccca attttcctct ctgctatcat ctcctctcct caatttttct tcaagaagat
     1021 taacaatcct ttctaactca cctatctccc catcatctat attcttaata gcctcttcta
     1081 atgcgtattt ctccaggact cttctaattt ccagaatctc cagaacttta tccttatcct
     1141 gatttatatg taaaataaga taggatgtat cattctttaa aggagcttta ataaagatac
     1201 cttttcctgg taagacctca ataagattga ggctctccat ctttcttaaa gcttctctta
     1261 ggagaggtcg gctaaccctg agctcgttag acaactctct ttcagagggg agcttatctc
     1321 caatctttag attactggaa gaggcatatt ctactattgc ttctattata ctttctgtta
     1381 actttattct attaactggt cttaccatct taccagagat aatagcataa gaaaaatact
     1441 atgtcaagca aatgtgaagc agatatcacc actatgatag agggaaagat aacgcagagt
     1501 taatattata gagaagagaa ctgaggctca caactcgcat ctaataatac tttatataaa
     1561 aagac
//
LOCUS       DAOTKU010000405         1561 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_64661_length_1561_cov_3.088313, whole genome shotgun sequence.
ACCESSION   DAOTKU010000405 DAOTKU010000000
VERSION     DAOTKU010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64661_length_1561_cov_3.088313"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="P5310_08235"
     CDS             <1..400
                     /locus_tag="P5310_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU33383.1"
                     /translation="GYRLYLFIKNFTVPEGEGEDADIASSIYRVSDYNKVISLLQLSP
                     YFYKERTPSDQEIFVCLKNPRSELIINNTGIVTPEEGYIDSDEEPGIGIVRVDKNYLE
                     VLAPTKKRLEAVEALLKEVAGEHITLEDMR"
     gene            405..1559
                     /locus_tag="P5310_08240"
     CDS             405..1559
                     /locus_tag="P5310_08240"
                     /inference="COORDINATES: protein motif:HMM:NF018004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HRU33384.1"
                     /translation="MAEVRPFIGLTYNREVINNLKDVISPPFDTIPPELHKNLYDRHL
                     YNIVRLELPSGEGEEKYINAGKILESWIESRILVPAEGARFYLYKERYILNGEERVLR
                     GLFGAVRLEPFDKRVILPHELTFPKPKEDRLNLLRATGSNISPILGIYFDDSDSSNRI
                     WEDTRSQKPLFEDEDFSFWEVEDDLEGMVALFEDKVILIADGHHRYETALEYKIEMES
                     RLKKTGPYSFVMFFLVEANTGGLSLLPTHRVIRGIPEQFDESILRSFHLEEVERPDLN
                     RDENICYLYRYGKFSMFRTESLNVVSLHKFLETFSELTIHYTHNMEEALSLVDSGGYD
                     LAFIVTPPSMDTVRKVVEKGDRLPHKSTYFYPKIGAGLVLYNHNLNLEMR"
BASE COUNT          493 a          255 c          367 g          446 t
ORIGIN      
        1 tggctataga ttatatctat ttattaaaaa tttcactgta ccggaaggtg agggagagga
       61 tgcagacata gcaagctcaa tatacagggt cagtgattat aacaaggtaa taagtttact
      121 tcagctttct ccttactttt ataaggaacg taccccatct gatcaggaga tatttgtctg
      181 tttaaagaat cccagaagtg agcttataat taataatacc ggaatagtaa ccccagaaga
      241 gggttacatt gatagtgatg aagaacccgg aataggtata gttagagtag ataagaatta
      301 tctggaggta ttggctccaa ctaaaaagag attggaagct gttgaggcat tactgaaaga
      361 agtggcaggc gaacatatta ctttagagga tatgcggtaa tactatggca gaggtcagac
      421 cgtttattgg tcttacatat aatagagagg ttataaataa cttaaaagac gtaatatctc
      481 ctccttttga taccatccct ccagagttac ataagaatct ttatgatagg catctttata
      541 atatagtcag gctagagttg ccatctggag aaggggaaga gaagtatata aatgcaggaa
      601 agatactgga gtcctggata gagagcagga tactggtccc agctgaaggt gccagattct
      661 atctctataa ggaaagatat attttaaacg gggaggagag ggtattacgt ggtctctttg
      721 gagcggtaag acttgaacca tttgataaaa gggttatact tccacatgaa cttacgtttc
      781 caaaacccaa agaagataga ctgaaccttc tcagagccac cgggtcgaat atcagtccca
      841 tcctgggtat atattttgat gattctgatt caagtaacag gatatgggaa gataccaggt
      901 ctcaaaaacc tctatttgag gatgaagact tctctttctg ggaagtggag gatgatctgg
      961 aagggatggt ggctctgttt gaggacaagg tcattcttat agctgatgga caccatagat
     1021 atgagaccgc ccttgagtat aaaattgaaa tggaatccag gttaaagaag accggtccat
     1081 acagttttgt aatgttcttt ctggtggaag ccaatacagg aggactgagt cttctcccca
     1141 ctcacagggt tattagggga atacctgagc agtttgacga atcaatctta aggtctttcc
     1201 atctggaaga agtcgaaaga cctgatttaa atagagatga aaatatatgt tatctctaca
     1261 ggtatggcaa gttttccatg ttcagaacag agtctctcaa cgtagtttcc ctgcataagt
     1321 ttctggaaac tttcagcgaa cttaccatac attatacgca taatatggaa gaggcgttat
     1381 ctcttgtgga tagcggaggc tatgatctgg cttttattgt tacccctccc tctatggata
     1441 cggtgaggaa agtggtggag aaaggggata gactccccca taagagtacg tatttttatc
     1501 ctaagatagg ggctggtcta gttttgtata atcataatct aaatttggag atgagataga
     1561 t
//
LOCUS       DAOTKU010000406         1560 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_64725_length_1560_cov_2.757475, whole genome shotgun sequence.
ACCESSION   DAOTKU010000406 DAOTKU010000000
VERSION     DAOTKU010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64725_length_1560_cov_2.757475"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="P5310_08245"
     CDS             complement(<1..555)
                     /locus_tag="P5310_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008879432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HRU33385.1"
                     /translation="MILLGLLIYPEFVLSKPSLKLGLEVLLTERPDLLRGKRIGLITN
                     QTGVDSKLESNISLFLEESGINLVALFAPEHGIRGERPAGEYVESYTDEETKLPVYSL
                     YGKTRKPTKDMLKGIDLLIFDMQDVGVRHYTYISTMALAMESAKENNMEFMVLDRPDP
                     LGGLNVEGPVLDPAFSSFIGIYPIP"
     gene            643..1278
                     /locus_tag="P5310_08250"
     CDS             643..1278
                     /locus_tag="P5310_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012582616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosylase/DNA lyase"
                     /protein_id="HRU33386.1"
                     /translation="MDKFEKILTGLKKIEGMAEPLVEERIGEFRRLGSSGSEEELFSE
                     LSFCVLTANWSAGGGIRAQKEIGISGFINLSQEELSRRLSELGHRFPHKRAEFIVKNR
                     HLINNLKEIISFSAPDARRLLVREAKGIGWKESSHFLRNVGQLGVAILDRHILKILHE
                     AGCIEDIPSGWTEKKYLSIEERFRELAERFGKPPGETDLYIWYMIKGKVEK"
     gene            1354..>1560
                     /locus_tag="P5310_08255"
     CDS             1354..>1560
                     /locus_tag="P5310_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014453415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HRU33387.1"
                     /translation="MRKMTEDSLKSAFAGESQAHMKYMIFSEIAEKEGKPNLARLFKA
                     IADAEKVHATNHLNALSLVKDSSEN"
BASE COUNT          498 a          287 c          349 g          426 t
ORIGIN      
        1 tgggatagga tagatcccta tgaaggagct gaaggcaggg tcaaggacag gtccttccac
       61 attgagacct ccaagagggt ctggtctatc aaggaccata aactccatat tattctcttt
      121 tgctgattcc atagccagag ccatagtgga gatataggta tagtgcctta ctccgacatc
      181 ctgcatatca aatataagca ggtctatccc ctttagcata tcctttgttg gttttctcgt
      241 cttaccgtac aggctgtata ctggaagttt tgtctcttcg tctgtataac tctccacata
      301 ctctccagcc ggtctctctc cccttatccc atgctccggg gcaaagagtg cgactagatt
      361 tataccagat tcttccagga aaagagatat attgctctcc agtttactat ccacacctgt
      421 ttgatttgtt ataagtccta tacgttttcc ccttaaaagg tcaggtcgtt ccgttaataa
      481 tacttcaagt cctaacttca ggctgggctt agacagtaca aattctggat atatcaaaag
      541 cccaagaaga atcaggacta aaatgccata tctcagcaag gctattaatc ctcctatgca
      601 ttttatttat gtcaagtata tcatttatga taaaatctat ctatggataa gtttgagaag
      661 atactgacag gattaaagaa gatagagggg atggcagaac ctctggttga agagaggata
      721 ggagagttca ggagattggg gagctctggc tcagaggaag agctcttttc agaattaagt
      781 ttctgtgttc tcactgctaa ctggagtgct ggtggaggca taagagctca aaaagagata
      841 ggaatttcag gatttataaa tctatctcaa gaagagttat ccagaagatt atctgaactg
      901 gggcaccgat ttccacataa gagggcagaa ttcatagtta aaaatagaca tctaataaat
      961 aacctgaagg aaatcatctc tttcagtgca ccggatgcca gaagattact ggtaagagag
     1021 gcaaagggga taggatggaa ggaatcaagt cattttctga ggaatgtcgg gcaattggga
     1081 gtagccattc tggaccgtca tatacttaaa atactccatg aggcaggatg tatagaggat
     1141 ataccatcgg gatggacaga gaaaaaatat ctatccatag aggagaggtt cagagaactg
     1201 gcagagaggt ttggtaaacc acctggagag acagacctct acatctggta tatgataaag
     1261 ggaaaggtag agaagtaatc cacttgactt tgtaagaata tattttataa taagatttaa
     1321 ttaataaaaa ctattgctaa ggaggtcgat aatatgagaa agatgacaga ggatagttta
     1381 aaatctgcat tcgctggcga gagccaggct catatgaaat acatgatctt ttctgaaata
     1441 gcagagaaag agggtaaacc aaacctggcc agactcttca aggctattgc agatgcggaa
     1501 aaagtccacg caacaaatca tctcaatgct ttatctctgg tgaaggattc ttccgaaaat
//
LOCUS       DAOTKU010000407         1556 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_64984_length_1556_cov_3.679547, whole genome shotgun sequence.
ACCESSION   DAOTKU010000407 DAOTKU010000000
VERSION     DAOTKU010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64984_length_1556_cov_3.679547"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="P5310_08260"
     CDS             complement(<1..405)
                     /locus_tag="P5310_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HRU33388.1"
                     /translation="MRERLLIPREDILRRIEELGDKISRDYTDKEPVLVCILKGAAYF
                     TVDLTRHLKIPFILDFMSMSKYSGSDSGVVRITKDLDIDIYERDTILIEDIVDTGLTL
                     GYLLRNLRTRGPNSIKVCTLLDCPVRRIVDIPV"
     gene            complement(402..1334)
                     /locus_tag="P5310_08265"
     CDS             complement(402..1334)
                     /locus_tag="P5310_08265"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HRU33389.1"
                     /translation="MDVKIITVSREFGCRGTEVAEKLAKELKFLLINKSYINDLMLKN
                     GLEEDQLESLEESSPRPFNETRKRYIELMNKLILELAEASPIVLLGRGGQYLFKNHPS
                     AFHIKIIGSIEERVRAVMTQYKLDRVGALKLITEQDRRRDLYINRVFGGDWNDLSLYS
                     LTINMDYFDVTGSVKMITRSIKLANRDNSAFSDVQTLETPKDATGSIPHFAHSSEEEF
                     AKLLDFYRIRWLYESKTFILGCDSEGNITSAFTPDFYLPDYDLYIELTTQKQRLVNYK
                     NRKIRRLRELYPDINIKMIYGRDYKNILRKFGLI"
BASE COUNT          452 a          330 c          242 g          532 t
ORIGIN      
        1 caccggtata tccactatac gtctgacagg acagtccaga agtgtgcata cctttataga
       61 attgggtccc ctcgtcctga gatttctcaa gagatatccc aatgttagac ctgtatctac
      121 aatatcttct ataagaattg tatctctttc gtatatatct atatcgaggt ctttggtaat
      181 tcttaccact ccagaatcac taccactgta tttggacatg gacataaagt ctagtataaa
      241 tggaattttg agatgtcttg tgaggtctac ggtaaaataa gccgctccct tcaggataca
      301 taccagaaca ggctctttat ccgtataatc tcttgaaatt ttatcgccaa gttcttctat
      361 ccttctcagg atatcctctc tcggtattaa taatctctct ctcatatgag tccaaactta
      421 cgaagtatat ttttataatc tctaccatat atcatcttta tatttatatc tggataaagt
      481 tctctcaatc ttctaatctt cctgttcttg taatttacca gtctctgttt ttgagtcgta
      541 agctctatat acaggtcata gtctggaaga tagaaatctg gcgtaaacgc agaagtaata
      601 ttgccttcac tatcacatcc cagaataaag gtcttagatt catagagcca ccttatcctg
      661 tagaagtcca gcagtttggc aaattcctct tcagaagaat gagcaaaatg agggattgag
      721 ccggtggcat ctttcggtgt ctccaaggtt tgaacatcag aaaatgcaga attatctcta
      781 tttgccagtt ttatagatcg ggtaatcatc ttaactgaac ctgtaacatc aaaatagtcc
      841 atatttatgg taaggctgta aagagacagg tcattccagt ctccaccaaa gaccctgttt
      901 atatacagat ctctccttct atcctgttct gttataagct ttaatgcccc caccctatcc
      961 agcttgtact gagtcatcac tgcccttact ctctcctcaa tagaacctat tatctttata
     1021 tgaaaggctg aaggatgatt cttaaatagg tactgtcccc ctcttccaag aaggacaatg
     1081 gggctggctt ctgctaattc cagtataagt ttgttcataa gttctatata tctctttctt
     1141 gtctcattga aaggacgcgg cgaactctcc tccaaggatt ccagctggtc ttcctccaat
     1201 ccatttttaa gcataaggtc attgatgtag cttttattta taaggaggaa ctttaactct
     1261 ttagcaagct tctcagctac ctctgtccct ctacatccaa attctctaga tacagtaatt
     1321 atttttacat ccatctttta tcacaaggat attttatcag aaaaaagaga gaagagaaat
     1381 accacaggat agtctctttt tcttaaagtt ttcttaataa tatcttatta ttctcttaat
     1441 acaggagttt tattatattt atggattaag aaataataaa attaattatg caatcaagtt
     1501 atctcttaaa ccctcctctt taaatatatt ttgggctagg gactcctagc ccaaaa
//
LOCUS       DAOTKU010000408         1555 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_65049_length_1555_cov_3.113333, whole genome shotgun sequence.
ACCESSION   DAOTKU010000408 DAOTKU010000000
VERSION     DAOTKU010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65049_length_1555_cov_3.113333"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(179..1195)
                     /locus_tag="P5310_08270"
     CDS             complement(179..1195)
                     /locus_tag="P5310_08270"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRU33390.1"
                     /translation="MEDKKWEARTDRGVTIYKIAEELGVSPSTVSRALNNTGRISQEL
                     REKIIELAQKYNYIPNEMGRGLRTRSSMLLGLMVPDIANPYYVNVARGAQDEADKYGY
                     SIAICNTDNDADLEKKRIITLVRKRVDGLLVFNFFNNKDTLELLSGFTIPIVLFNPPP
                     PGYRFVHIKHDTSVINSLLTYLSGKGYTSIAHIAGDQRTIVGRLRLKHFIHFMRSHNL
                     PLREEWTIESDFTQKGGYEAMKTLLMLEEKPRAVWASNDLMAIGAMEAIKESGLSIPK
                     DIAVAGYDDIEYASIVTPKLTTVYKPKYELGSIATGILINLIKGEGVETVLLKDKLII
                     RESA"
     gene            complement(1237..>1555)
                     /locus_tag="P5310_08275"
     CDS             complement(1237..>1555)
                     /locus_tag="P5310_08275"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HRU33391.1"
                     /translation="KRHEEYWHNWCHVISNALVVVSSLLYSGLDYEKGITYAVSCGLD
                     TDCNGATTGSILGTIIGAKDLPEKWISPINDKIESGVARYNLCQVSDLARKTVEAIKI
                     LSR"
BASE COUNT          421 a          343 c          271 g          520 t
ORIGIN      
        1 taacggcttc tcttcataat ctcttaaaat agcctgtcct ggtgcgatgg ctattaactc
       61 ttttgacatc ttatatctcc tttcctttca aattacattt atcataaggt aaacacctga
      121 aagctgaaga attgccagga tatatatgaa cttcctggta ttactgtaga aaatttattt
      181 atgccgattc cctaataata agcttatcct taagcaatac cgtttctact ccttctcctt
      241 ttatcagatt tattaatatt cctgtcgcta tggagccaag ttcatatttt ggcttataga
      301 ccgttgttag ttttggagtc actatagagg catattctat gtcatcgtag ccagctactg
      361 ctatatcttt aggtatagag agtccagatt cttttatagc ctccatcgcc cctattgcca
      421 tcaggtcatt tgaggcccaa actgcacggg gcttctcttc caacatgagc aatgttttca
      481 tagcctcata gccacccttt tgggtgaagt cactctcaat ggtccattcc tctcttaaag
      541 ggagattgtg ggacctcata aaatggataa aatgcttgag acgaagcctt ccaactatag
      601 ttctctggtc tccagctata tgggctatag atgtgtaacc tttgccagat agataggtca
      661 atagggaatt tataacagaa gtatcatgct ttatatggac aaatctatag ccaggagggg
      721 gaggattaaa gaggactatg gggatagtaa agccacttaa gagttcgaga gtatctttgt
      781 tattaaagaa gttaaagaca agtaatccat ctacccgctt tctaaccagt gtgataattc
      841 gtttcttctc taaatccgca tcattatctg tattgcagat agctatggaa tatccatact
      901 tatctgcctc atcctgagcc cctctagcaa catttacata gtagggattg gctatatcgg
      961 gtaccattaa accgagaagc atggaagacc tggttctaag ccctctgccc atttcatttg
     1021 gaatatagtt atatttttgg gcaagttcta tgattttctc ccttaattcc tggcttattc
     1081 taccagtatt atttagcgcc ctggaaacag tagatggaga aacaccgagt tcttctgcaa
     1141 ttttgtatat agtaactcct ctatctgttc ttgcttccca tttcttatct tccatctatt
     1201 aaaattcttt ctctcatctt ctcaaatccc cataagttat ctagatagta tcttgattgc
     1261 ctctactgtc tttctggcta aatccgatac ctgacaaagg ttatacctgg caactcctga
     1321 ctctatcttg tcatttatag gacttatcca cttctctggg agatctttag ctcctataat
     1381 ggtcccaagg atggagccag tagttgctcc gttacagtca gtatcaagcc cacaactcac
     1441 cgcataggtt ataccctttt cataatcaag tccactgtat aaaagtgagc tcacaactac
     1501 aagggcattt gagattacat gacaccagtt gtgccagtac tcttcatgac gttta
//
LOCUS       DAOTKU010000409         1551 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_65290_length_1551_cov_2.203209, whole genome shotgun sequence.
ACCESSION   DAOTKU010000409 DAOTKU010000000
VERSION     DAOTKU010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65290_length_1551_cov_2.203209"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..783)
                     /locus_tag="P5310_08280"
     CDS             complement(<1..783)
                     /locus_tag="P5310_08280"
                     /inference="COORDINATES: protein motif:HMM:TIGR03168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HRU33392.1"
                     /translation="MIITVTPNISLDKTLVVENFEIGKTHRVKDITAIPGGKGVNASR
                     ALLGMGYIAPVLGFIGGNTGQSILNMFDHEGIKYDVVKINGETRTCYNVVDPINHTQT
                     EVLENGPIVTDNDISLLEAKITGYVKEDTIVCMGGSLPCRAPQDLYVRLVESINLKGG
                     RVILDASGDRLKEGIRGNPFAVKPNRAEVESILGFSLDSDENIKKALDYFVAARITLP
                     VISMGKDGVAFYYDGKYYRITPPVLKALNAVGSGDSTVAGIAL"
     gene            832..918
                     /locus_tag="P5310_08285"
     tRNA            832..918
                     /locus_tag="P5310_08285"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:866..868,aa:Leu,seq:cag)
BASE COUNT          396 a          388 c          245 g          522 t
ORIGIN      
        1 caatgcaatt cctgccactg tggagtcccc agagccaaca gcattcaatg ccttaagaac
       61 aggtggagtt atcctgtaat atttgccatc atagtagaat gctacaccat ctttgcccat
      121 agagatgaca ggtaaagtta ttctagcagc aacaaagtag tccagagctt ttttaatatt
      181 ctcatcgcta tccaggctaa atcccaggat tgactctacc tcagctctgt tgggttttac
      241 agcaaaagga tttcctctta taccctcttt taatctatct ccacttgcat ccagaattac
      301 tcttccccct ttaagattta tagactctac cagtctgacg tagaggtcct gaggtgctct
      361 acagggtaaa ctccctccca tacatactat ggtgtcttct tttacataac ctgtaatttt
      421 tgcctctaaa agacttatat cattgtcggt tactattggt ccattctcca aaacctctgt
      481 ctgggtgtga ttaatagggt ccaccacatt gtaacaggtc cttgtctccc catttatttt
      541 aaccacatca tattttatac cctcatggtc aaacatattc agtatgcttt gaccggtatt
      601 tcccccgata aagcccagta caggggctat atatcccatc cccagaaggg ctctggatgc
      661 atttacccct tttcctccag gaatagcagt aatatccttt accctgtggg tctttcctat
      721 ttcaaaattt tctaccacga gagttttatc cagcgatata tttggggtta ctgtaattat
      781 catacttatc ctccttgaca tttttattat ttaaactata atggttactg tgccgaagtg
      841 gcggaattgg tagacgctct ggattcaggg tccagtggat gcaagtccgt accggttcga
      901 gtccggtctt cggcaccact tttttctgtt actatatctt ttaatgggac ttctttttgc
      961 catccatttt taagctctaa tattacggtg tttctcagga catttatccc tattactctc
     1021 ccttcaccaa gtgatgaatt caccacagaa cctatctccg ggaagttttt aattacagaa
     1081 ctataaaact cagcctcaaa ccttaaacag cacattaatc ttccacatac tcctgaaatt
     1141 ttggtaggat ttagtataag tccctgctct tttgctattt ttacagatat aggggcaaat
     1201 tcttctaaaa attcacagca acaggctacc tttccacaag gaccaagtcc ctttatcaac
     1261 tttatctcat ctctagctcc tatctgcctg agctctactc tattcataag tctatgtgcc
     1321 aggtctttta ccagctctct gaaatctatt ctcccctcgg ctgtaaagaa gcatattata
     1381 cgttccggat ccacaccaaa gtcaaccctt acaagtttca tctggagatt ataagccctt
     1441 atacgttctt ttactgtgtc aaaaatctct tttcgcctta tattcctcgt cttagccgaa
     1501 gattcttcct ctgtggaagc ctttctgata atcgccttta agctctcagt c
//
LOCUS       DAOTKU010000410         1549 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_65392_length_1549_cov_2.931727, whole genome shotgun sequence.
ACCESSION   DAOTKU010000410 DAOTKU010000000
VERSION     DAOTKU010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65392_length_1549_cov_2.931727"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            90..>1549
                     /locus_tag="P5310_08290"
     CDS             90..>1549
                     /locus_tag="P5310_08290"
                     /inference="COORDINATES: protein motif:HMM:NF020084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST C-terminal domain-containing protein"
                     /protein_id="HRU33393.1"
                     /translation="MLKVGLGFSMLSDSYKAGVQSAREAVSKSGDPSFTIFFNTLDYD
                     PQAVFKGVKEAVKDSRIIGATSKGIIVYDDIFLKGIGVLTVGGDGLNVKTFALEEFKI
                     DDIEKGEKTGEFLLESGIRRGTVIIFFANGFIDPYKMLLGLYNTMGPEFSYIGSGNEG
                     NIRNQCAFKYTERGVNEGPLVAAVIEGIDFSVALSHGFTVSGDPLVITETNKNRIVEI
                     DGIPALDAYAKRLNKDSDINLLSHMFLHPLGFPNLSGDYIIRDPMDINPDKSISFPIE
                     IPKGAVGYVMKGRINNLIKSSGDTARRAIQKVKDPQFALIIDCISRHSLMKKKFKMEL
                     EAIRNSMGADIPVLGMLSWGEIGSQDSAPMSHNKTTVVAVAGEKIKDIEEIRKETNIQ
                     TLSADLSILHEIASLSYSGSFKRFSEDAIEKTIRLFGARRSALLEKTSGGYRLLSCWG
                     FKDLDEILGVLDKENSRSITFYLGECGKYGALYLEK"
BASE COUNT          507 a          232 c          353 g          457 t
ORIGIN      
        1 acaggatata aaaacgattg ctctcttcta ttaacacaaa gaaaaaattt cttttatgtt
       61 ataattaact aaaaagtagg aaggatttta tgttaaaggt tgggcttggt ttcagtatgc
      121 ttagcgattc ttataaggca ggggtacagt cagccagaga ggctgtttca aaatctggcg
      181 atccgtcctt tactatcttt ttcaataccc tagattatga cccccaggct gtatttaagg
      241 gggtaaaaga agcagtaaaa gattccagga ttataggagc aaccagtaag ggtatcattg
      301 tctatgatga tatctttcta aaagggatag gtgttttaac tgtaggaggg gatgggctta
      361 atgttaaaac ctttgcacta gaagaattta aaatagatga tattgaaaag ggagaaaaaa
      421 ctggagagtt cctgctggaa agtggaataa ggagaggaac tgttattatc ttttttgcta
      481 atggctttat agatccttat aagatgttgc ttggattgta taatactatg ggtccagaat
      541 tttcatatat tggcagtgga aatgagggga atataagaaa tcaatgtgca tttaaatata
      601 cagaaagggg tgtaaatgaa ggacctctgg tggctgctgt tattgaaggg atagattttt
      661 cagtagcttt atctcacggt tttacagtct ctggagaccc tctcgtaata actgaaacta
      721 acaagaacag aatagtagaa atcgatggaa tccctgcttt agatgcgtat gccaaaaggc
      781 tcaataaaga ttctgatata aatctgctgt cacatatgtt tttacatcct ctaggtttcc
      841 caaatctctc cggggattat ataatacgtg accctatgga tattaatcct gataagagta
      901 tctcgtttcc gatagagata cctaaggggg ctgtgggtta tgtgatgaag ggacggataa
      961 ataatctaat taaatcttca ggagatacag ccagaagggc tattcaaaag gttaaggacc
     1021 ctcagtttgc acttatcatt gactgtatat ccaggcattc acttatgaaa aagaagttca
     1081 agatggaatt agaagcaata aggaattcta tgggcgcaga tattcctgtg cttggtatgc
     1141 tttcctgggg agagattggt tctcaagact ctgcgccgat gtctcataat aagaccactg
     1201 tggtagcagt tgcgggagaa aagatcaaag atatagagga aatcaggaaa gaaacgaata
     1261 ttcagacgtt aagtgcggac ctatcaatac ttcatgagat agcatcactc tcttactctg
     1321 gttcatttaa aagattttca gaagatgcca tagaaaagac tataagactt tttggcgcca
     1381 gacgttctgc acttttagaa aaaacaagtg gaggatatag actactttca tgctggggat
     1441 tcaaggattt agacgagata cttggagtgt tagataaaga aaactctaga agtataacct
     1501 tctatctggg tgagtgtgga aaatatggag ctctctatct agaaaagga
//
LOCUS       DAOTKU010000411         1548 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_65459_length_1548_cov_2.848627, whole genome shotgun sequence.
ACCESSION   DAOTKU010000411 DAOTKU010000000
VERSION     DAOTKU010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65459_length_1548_cov_2.848627"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="P5310_08295"
     CDS             complement(<1..225)
                     /locus_tag="P5310_08295"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HRU33394.1"
                     /translation="MFSIDFDKYRIIDLSLDVIPPGTEDRPFKVEKGLLSDRAYMHKV
                     WTHSHVGTHIEASSHFFDDGKDIDEYPLDAF"
     gene            complement(243..1115)
                     /locus_tag="P5310_08300"
     CDS             complement(243..1115)
                     /locus_tag="P5310_08300"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRU33395.1"
                     /translation="MLRLKGTIPAVLTPFDENYEIDENGFQELCEYLIENKVSGLFVA
                     GTTGEGPLLSLEERARLFKLVVKISNGRVPVICHTGSISLTETSLLIRSAIEAGADAV
                     GIVTPYYYRLDEKAMEEYYSKVLEKIKGFPVFLYNIPGLTNNYMSSSLAEKLFSQGKI
                     AGIKDSSGNITTLKSFIDISPDIQVISGADELFLLALTLGAVGAVSSTANVFPEVFNN
                     IYNAFLSRDMDVAIENQRKLNQLCKIFQYGKLISAYKYALYLKGLKISRVRLPQRELT
                     EEEKSMMQEELKKL"
     gene            complement(1126..>1548)
                     /locus_tag="P5310_08305"
     CDS             complement(1126..>1548)
                     /locus_tag="P5310_08305"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HRU33396.1"
                     /translation="GYITTPRGCIQPAVVELEDGHLLCFLRTRDSFIFRSESYDYGRT
                     WSPPCSTSLKNPNSGIDLIKLIDDRLIVIFNDSFDRRTPLNAGISEDNGESWEIFDLE
                     REDGEYSYPGIIQIRDKLVHLVYTYRREAIKYVVMEID"
BASE COUNT          465 a          353 c          244 g          486 t
ORIGIN      
        1 aaaggcatcc agtggatact catctatatc cttaccatca tcaaaaaagt gagaactggc
       61 ttcaatatgt gtgccgacgt gactgtgtgt ccagaccttg tgcatatagg ctctatctga
      121 gagtaatccc ttttcaacct taaatggtct atcttctgta cctggaggta taacatccag
      181 cgaaagatcg atgattctgt acttatcaaa atctatactg aacatcttat caccctctct
      241 aattaaagtt ttttaagctc ttcctgcatc atactctttt cttcctcagt aagttctctc
      301 tgtggtaacc tgactctact tattttaaga ccttttagat aaagtgcgta cttataagca
      361 gatatgagct tcccatactg gaatatctta catagctgat tgagtttacg ctgattttct
      421 atagccacat ccatatccct ggagagaaat gcattataaa tattattaaa gacctcagga
      481 aatacgttgg cggtagaaga aactgctcct actgcaccta aggtaagagc aagaagaaac
      541 aattcgtcag ctccagagat tacctgtata tccggagata tgtctatgaa actcttaagt
      601 gttgtaatgt tccctgagct atcctttata ccagctattt tcccctgaga aaaaagcttc
      661 tctgctaagc tggaagacat gtaattattt gtaagcccgg gaatgttgta gagaaatacc
      721 gggaaaccct ttatcttttc caatacttta gaataatact cctccattgc cttctcatcc
      781 aacctgtaat aatatggggt aacaattccg acagcatctg ctccagcctc tatagctgat
      841 ctaataagaa ggctcgtctc tgtaagagag attgaaccgg tatgacatat taccggaact
      901 cttccattag aaatcttaac gacaagtttg aagagtctgg ctctttcctc taaagataag
      961 agaggaccct caccggtcgt tccagctaca aataacccac tcaccttatt ctcaataaga
     1021 tactcacaca actcttgaaa gccattttca tctatctcat aattctcatc aaaaggggta
     1081 agaaccgcag gaatagttcc ttttaatctt aacattttga cctccttaat caatctccat
     1141 aactacatac tttatagctt ctcttctata agtatacact aaatggacta atttatccct
     1201 gatttgtatt atgcctggat atgagtattc tccatcctct ctctccagat cgaagatctc
     1261 ccaactttct ccattatctt cagaaatacc agcgtttaat ggggtcctcc tatcaaaact
     1321 atcattaaag ataacaatca atctatcatc tataagtttt ataaggtcta tcccgctatt
     1381 tggatttttc aaagatgtag aacagggagg actccacgtc ctgccatagt cgtaagattc
     1441 gcttctaaat atgaaactat ccctggttct caagaagcat aacagatgtc catcttccaa
     1501 ttctaccacc gcaggttgga tacaacctct aggagttgta atatatcc
//
LOCUS       DAOTKU010000412         1546 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_65562_length_1546_cov_3.375587, whole genome shotgun sequence.
ACCESSION   DAOTKU010000412 DAOTKU010000000
VERSION     DAOTKU010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65562_length_1546_cov_3.375587"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="P5310_08310"
     CDS             complement(<1..545)
                     /locus_tag="P5310_08310"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflK"
                     /protein_id="HRU33397.1"
                     /translation="MDEVFDWEKMPEEGKRRNIKPSSIIFGIFIGIILIIILSTTFYT
                     VNTDEAGVIKTFGSYTKVTGPGIHAKWFWPIQAVEKVSIEKVNRIEIGFRTTGKDSDG
                     NAIYSDVPDEKIMVTMDENIVEVEFIVQYIVRDPVAYLFNVDDPVETVRKTSWSAMRT
                     VVASNTVDDVLTIGKENIQNRT"
     gene            642..1493
                     /locus_tag="P5310_08315"
     CDS             642..1493
                     /locus_tag="P5310_08315"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004079927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRU33398.1"
                     /translation="MDKRPYGKTGEMLSIIGFGGIVAAELPQEEANSIVALAIDRGVN
                     YFDVAPTYMDAEERLGIALKGKRNNIFLACKTGKRTKEEARKELENSLRLLNTDHFDL
                     YQLHSMTTDEDFEVAMGPGGAMETFVKAKDEGLIRYIGFSAHSVEVALKLLDAFQFDS
                     VLFPLNWVNYFNANFGPQVVEKAMAKGVARLALKAMAKTVIPEGQSRKYEKCWYLPVD
                     EPELASLALRFTLSQPITAAIPPGEPKLFKMALDIAEKFTPITEEEIRLLKEKAKGLE
                     PIFRLRS"
BASE COUNT          434 a          300 c          314 g          498 t
ORIGIN      
        1 gtacgattct ggatattctc tttccctata gtgagaacat catctacagt gttacttgct
       61 actactgtcc tcatagcaga ccatgatgtt tttcttacgg tttctaccgg atcatccaca
      121 ttaaacagat atgctaccgg gtctcgaact atatactgga ctataaattc aacttcaact
      181 atattttcgt ccattgtcac cattatcttt tcatctggga catcactata aatagcattt
      241 ccatcggaat cttttcctgt agttctaaaa cctatctcta ttctgtttac tttttctatg
      301 gagacctttt ctaccgcttg aatgggccag aaccattttg cgtgtatccc tggtcctgtg
      361 actttagtgt aggatccaaa ggtctttata acccctgcct catctgtatt aacagtatag
      421 aatgtggtgg ataatattat gatcagtatt atccctatga atatcccgaa tattatagaa
      481 gaaggtttta tgtttcgtct tttaccctct tctggcatct tttcccaatc aaagacttcg
      541 tccataattt tccatctcct ttttttcagt ataacatatt atagacaatt tatctaagta
      601 atagtataat tttactaaaa tctattattt aggaggtcat tatggataaa agaccgtatg
      661 ggaaaacagg cgagatgtta tccattatag gttttggggg tattgtagca gctgaacttc
      721 ctcaggaaga ggctaacagt atagttgctc ttgctataga cagaggtgta aattattttg
      781 acgttgctcc cacatatatg gatgctgaag aaaggctggg tatagctctt aagggtaaga
      841 ggaataatat attcctagcg tgtaaaaccg ggaagagaac gaaggaagag gcacgtaagg
      901 aactggaaaa ctctttaaga cttttaaata ccgaccactt tgacctttat cagctccatt
      961 ctatgactac cgatgaagat ttcgaagtag ctatgggtcc gggtggagcg atggagactt
     1021 ttgtcaaggc gaaggatgag ggattaataa ggtatatagg attttcagct cattcggtag
     1081 aggttgcatt aaagttactg gatgcctttc aatttgattc tgtcctcttt cccctgaact
     1141 gggtgaacta tttcaatgct aattttggtc ctcaagtagt agagaaggct atggctaaag
     1201 gagtagccag acttgccctt aaggctatgg ctaagacggt tataccagaa gggcaatccc
     1261 gtaagtatga gaagtgctgg tatcttcctg tcgatgagcc agaacttgct agtctggctt
     1321 tgagatttac cctctctcag cctataacag ctgctattcc acctggagag cccaaactct
     1381 ttaagatggc tctggatata gctgagaagt tcaccccaat tacagaagag gagataagac
     1441 tgttgaaaga gaaggctaag ggtctggaac cgatattcag gcttcgaagc tgatttccaa
     1501 ctcatacgta cagttgcact tatctatctt tatatgttat aatgcc
//
LOCUS       DAOTKU010000413         1532 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_66421_length_1532_cov_2.859174, whole genome shotgun sequence.
ACCESSION   DAOTKU010000413 DAOTKU010000000
VERSION     DAOTKU010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66421_length_1532_cov_2.859174"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1532)
                     /gene="rpoB"
                     /locus_tag="P5310_08320"
     CDS             complement(<1..>1532)
                     /gene="rpoB"
                     /locus_tag="P5310_08320"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HRU33399.1"
                     /translation="DIDHLGNRRVRRIGEMLQNQFRIGLLRMERMIKERMSLQPDMEK
                     AIPQMLINIRPLTAAIKEFFNSSQLCQFMDQTNPLAELTNKRRVSALGPGGLNRDRAG
                     FEVRDVHHTHYGKLCPIETPEGPNIGLINSLATYARVNDLGFIETPYWKVENGRVTSQ
                     IDYLTADEEKKYRIAQANINIDKDGYILDEEVPCAYGGTFIIAPRDLVQYMDVSSSQI
                     VGVSAALVPFLEHDDASRALMGANMQRQAVPLIKPKAPVVATGLEMIVPKYSGYLVSA
                     KRPGVVKSVSADRVIVETDDGEEDMYKMLKFIRSNQSTCINNRVIVNKGDRLDAGDAI
                     ADGMAMDYGDLALGQNVLVALMPWEGYNYEDAILISERLVRDDIYTSIHIEEYEIEAR
                     DTKAGQEEITREVPNVSEESLKNLDEFGIIRVGSEVQPGDILVGKVSPKGETELTPEE
                     RLIRQIFSEKAREVRDTSLRLPPGEKGIVIKTQIFSRENFDDLPPGVLKVVKIYVAQK
                     RK"
BASE COUNT          398 a          414 c          234 g          486 t
ORIGIN      
        1 ccttcctctt ctgagccaca taaatcttta ccacctttaa aaccccaggg ggaagatcat
       61 caaaattttc tctggaaaaa atctgcgtct ttatcactat ccctttctct ccaggaggaa
      121 gccttagaga tgtgtctcta acttctcttg ccttttctga aaaaatctgc cttataagtc
      181 tctcctctgg agtaagctct gtttccccct tgggagaaac ttttcccaca aggatatcac
      241 caggctgaac ttcactacct acccttatta ttccaaattc atctaaattt ttcaaagact
      301 cttcactaac attgggtacc tctctggtta tctcctcttg tccagccttc gtatccctgg
      361 cttctatctc atactcctct atatgaatag aggtatatat gtcgtccctt accagtcttt
      421 cactaatcag aatggcatct tcatagttat aaccctccca gggcataaga gcaactaaca
      481 cattctgccc caaagccaga tctccatagt ccatcgccat cccatcagct atcgcgtccc
      541 cggcatctaa cctgtcaccc ttattcacaa taaccctatt gtttatacag gtactctggt
      601 tagacctgat aaactttagc attttataca tatcttcttc cccgtcgtct gtctctacaa
      661 tcaccctatc cgcacttaca ctctttacta ctccaggtct ttttgccgat acaagataac
      721 cagaatattt ggggactatc atctccaatc ctgtagctac cactggagcc tttggcttta
      781 ttagaggaac cgcctgccgc tgcatattag ctcccatcag cgctctgctg gcatcatcat
      841 gctccagaaa tgggacaaga gcagcactga caccaactat ctggcttgaa gacacatcca
      901 tatactgtac caaatccctt ggagctatga taaatgtacc accataggcg cagggtactt
      961 cttcatccaa gatataaccg tctttatcta tatttatatt ggcctgagct attctgtact
     1021 tcttttcttc atcagcggta agataatcaa tctgacttgt tactctacca ttttctactt
     1081 tccagtatgg tgtttctata aaaccaagat catttactct ggcataagtg gctaaactgt
     1141 ttataagtcc tatattggga ccctccgggg tttctatcgg gcataatttc ccataatggg
     1201 tatgatgaac atcccgcact tcaaaaccag ccctatccct gttcaatcca cctggaccca
     1261 aagcactgac tcttctctta ttcgtcaact cggctaaagg attagtctgg tccataaact
     1321 ggcatagctg actcgaattg aaaaattctt ttatagctgc agttagaggt cttatattaa
     1381 taagcatctg aggaatagcc ttttccatat ccggttgtaa gctcattcgt tccttaatca
     1441 ttctttccat tcgaagcaaa cctattcgga actgattctg aagcatctct ccaatacgtc
     1501 ttacacgtct attacccaga tggtctatgt ca
//
LOCUS       DAOTKU010000414         1527 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_66735_length_1527_cov_2.893342, whole genome shotgun sequence.
ACCESSION   DAOTKU010000414 DAOTKU010000000
VERSION     DAOTKU010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66735_length_1527_cov_2.893342"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..968
                     /locus_tag="P5310_08325"
     CDS             <1..968
                     /locus_tag="P5310_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010149701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRU33400.1"
                     /translation="NLHADIYKEDSLAELVGVCDIIPERAEKAGKRLGVPWYTDAQTM
                     LRELSPDVCSVATGGYEYSSDHFEPTIQALEAGCHVLCEKPICNDIVKAQKMVDLARE
                     KNLCFAIDLNHRFTPAFRTAKRWFDEGKIGALLFINMSLWIGKFQDFESPYYHLKALN
                     PHSVNIMQQFGGQVEKVQCFAMKAPGRTIWSTASINMKFKNGAVGHLTSSYDIERGHP
                     MERCEVAGIRGRLVIEDMWREATLYPAGDLEKRVYTNPVFGGFRDFNDTFRDRIHKFL
                     EEITNGVRPEDIDGSGVEGLEAQRVIEAAIRSLDTGKIIDVEDVR"
     gene            complement(990..>1527)
                     /locus_tag="P5310_08330"
     CDS             complement(990..>1527)
                     /locus_tag="P5310_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU33401.1"
                     /translation="TIHIPLIIKIEDKFRGRKISPLVQNIDIMPTILELLDMEKDIKT
                     DGQSLIPLINSECEYLYREIVSTECTWRAGISIRTEGWKFIKTIDQGLYEKPSRELYN
                     LKDDPDEEVSLADKEKEILDELELRLTRWLDSKLKNKPNPITIQTEKGIPAKKWVENV
                     LKDLHLTWDDWVEKQRFI"
BASE COUNT          441 a          268 c          345 g          473 t
ORIGIN      
        1 gtaatctaca tgcagatata tataaggaag acagtctggc agaacttgta ggggtctgtg
       61 atattatccc tgagagggca gaaaaagctg gaaagaggtt gggtgttccc tggtatacag
      121 atgcacaaac aatgctaaga gagctttctc ctgatgtgtg cagtgttgct actgggggct
      181 atgaatactc cagtgaccac tttgaaccaa caatccaggc attggaagca ggatgtcatg
      241 tactgtgtga aaaaccgata tgtaatgata ttgttaaggc tcagaagatg gtagatctgg
      301 caagggagaa gaacctctgt tttgctatag atttaaacca cagatttacc cctgcattca
      361 ggactgctaa gagatggttt gatgagggta agataggagc tttgctcttt ataaatatgt
      421 cattatggat tggtaaattt caggactttg aatcccccta ctatcatctt aaagctctaa
      481 atccacattc agtgaacata atgcagcaat ttggcggtca ggtcgagaag gtacagtgct
      541 ttgcaatgaa ggctccagga agaaccatat ggtccacggc atctatcaat atgaaattca
      601 aaaatggagc agtaggtcac cttaccagca gttacgatat agaaagagga catcccatgg
      661 agagatgtga ggttgctggt ataaggggac gtcttgtaat agaggatatg tggagagaag
      721 caacactgta tccagccggt gatctggaga agagagtata tacaaatccg gtatttggtg
      781 gctttagaga ttttaatgat acatttagag ataggattca caagttctta gaagagataa
      841 ctaatggtgt aagacctgaa gatatagatg gatcaggcgt tgaagggttg gaagctcaga
      901 gggtaataga agcggctata agatctctag atacgggaaa gattatagat gtagaagatg
      961 taaggtaggg cttcaagccc tacctttatt tatataaatc tctgtttttc tacccagtca
     1021 tcccaggtta gatggaggtc tttgaggaca ttctccaccc atttttttgc tgggattccc
     1081 ttttcagttt gaatcgttat gggatttggt ttatttttta atttactatc aagccatctg
     1141 gtcagtctta gctccagctc atctaatatc tctttctctt tatcagccag gctgacctct
     1201 tcatcaggat catctttcag gttataaagt tctctggatg gtttctcata taaaccctgg
     1261 tctatcgtct ttataaactt ccatccttcc gtccggatag atattccagc tctccaggta
     1321 cattctgtag agacaatttc cctgtataga tattcgcatt cactatttat cagtggtata
     1381 agggattgac catctgtttt tatatccttc tccatatcca gcagttctag aatggttggc
     1441 attatatcta tattctgaac aaggggagag atttttctac ctctaaactt atcttcgatt
     1501 tttattataa gtggtatatg aatggta
//
LOCUS       DAOTKU010000415         1518 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_67309_length_1518_cov_3.328093, whole genome shotgun sequence.
ACCESSION   DAOTKU010000415 DAOTKU010000000
VERSION     DAOTKU010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67309_length_1518_cov_3.328093"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(141..455)
                     /locus_tag="P5310_08335"
     CDS             complement(141..455)
                     /locus_tag="P5310_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU33402.1"
                     /translation="MSKKELLERLSEIDKKILSLFIPVEISDLVMEREKLLESVLEIE
                     LSLQECYALEESNRKIMQHLKEIEMDLERRLGELKQYSSLYKSYYLSRYNLKDNLLSE
                     RV"
     gene            complement(452..817)
                     /gene="fliS"
                     /locus_tag="P5310_08340"
     CDS             complement(452..817)
                     /gene="fliS"
                     /locus_tag="P5310_08340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HRU33403.1"
                     /translation="MAVISPYNQYEVTKVLTSSPLEQVILLYKKSINLLEGALENLDE
                     DELLFSENIGKACRIIEYLLSVLNLEGGGVIAENLAKLYQYSILTLTTSNINRDRDGI
                     EEVKGILTGLLEGWEGIRK"
     gene            complement(817..>1518)
                     /gene="fliD"
                     /locus_tag="P5310_08345"
     CDS             complement(817..>1518)
                     /gene="fliD"
                     /locus_tag="P5310_08345"
                     /inference="COORDINATES: protein motif:HMM:NF018852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HRU33404.1"
                     /translation="HLTSTIRSLEDIGISIGNPGEVSIDEILAGKLTIDYEALNKALT
                     ESPETVADLFSKSYVYVQDEVPQGTIDGTNTRFTLNNKSISYDIRPMVYSDGVLLTQV
                     FGETTPEPGQFKLDYTTGTLILGEAPTSSVKVSYGYNTTGETAGIAVRINNLVTNYTK
                     DITGIIPYTINTLTTEYKELNELISDTNIRLSLYEENLIRQFVALEEAIAKVQSQSSF
                     LSSYLTGLQKQGGSK"
BASE COUNT          391 a          337 c          272 g          518 t
ORIGIN      
        1 ataaagcttg atgttatagg gtggatagga cagctgagag gctttgttat ggagtccaaa
       61 gaggcgtacc cttcaggtct atccagattt ttcaagagaa aagacgagta cgagacttaa
      121 agaggacccg ggattttaca tcataccctt tcactcaaca ggttatcttt taagttatac
      181 cttgacaggt agtagctttt atagaggctt gaatattgct tcagctctcc aagccttctc
      241 tccaggtcca tctctatctc tttcaggtgt tgcattatct ttctgttaga ttcctccagg
      301 gcgtaacact cttgcagact taattctatc tcaagtacag actctaaaag cttttctctc
      361 tccataacca gatcagatat ctcaacaggg ataaaaagag agagtatttt tttatctatc
      421 tctgataatc tttctaataa ttccttttta ctcactttct tataccctcc catccctcta
      481 aaagtccagt taatatcccc tttacctctt ctataccatc tctgtctcta tttatgttac
      541 tggtagtcag ggtaaggatc gaatactggt aaagtttggc aagattttct gctataacac
      601 ctcccccttc cagattcagg acacttaata gatattctat tatccggcaa gccttaccaa
      661 tattctcact gaataataat tcatcctcat caaggttttc cagagcccct tccaggaggt
      721 ttatactctt tttatagagc aaaataacct gctccagagg tgaactggtc agaactttag
      781 ttacctcata ctgattatag ggacttatta ccgccattat ttgctacctc cttgtttctg
      841 tagtcctgta agataactgg aaagaaagct actctgagat tgcacctttg caatcgcctc
      901 ttccagggcg acaaactgcc ttatgagatt ctcttcatag agagagagcc ttatgtttgt
      961 atcacttatc agttcattaa gttccttgta ctctgtagta agtgtattta tcgtatatgg
     1021 gataattcca gttatatctt ttgtgtaatt tgttaccaga ttgtttatcc ttactgcaat
     1081 ccctgccgtc tctcctgttg tgttatatcc gtaagagacc ttgacacttg aagttggagc
     1141 ctctcctagt ataagtgttc cagtagtata atccagctta aattgtcctg gttccggggt
     1201 ggtctctcca aatacctgtg tgagcaatac tccgtctgaa tataccatgg gtcttatatc
     1261 ataggatatc gatttgttgt ttaatgtgaa cctggtattt gtcccatcta tagtcccctg
     1321 tggtacctca tcctggacat acacgtatga tttggagaac aggtctgcca cggtctcagg
     1381 tgattccgtc agggctttgt tcaatgcctc ataatctata gtcagtttac cagctaatat
     1441 ctcatctatt gaaacctctc cagggttgcc tatggaaatt cctatatcct caagagacct
     1501 tatggttgag gtaagatg
//
LOCUS       DAOTKU010000416         1518 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_67341_length_1518_cov_2.192071, whole genome shotgun sequence.
ACCESSION   DAOTKU010000416 DAOTKU010000000
VERSION     DAOTKU010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67341_length_1518_cov_2.192071"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /locus_tag="P5310_08350"
     CDS             complement(<1..667)
                     /locus_tag="P5310_08350"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU33405.1"
                     /translation="MNPIIKRVGDNLFSYIKTIWSFIIQFFPRVIRSRELVWITVFLF
                     YFGILVFDFLPSSIEVAVGQVAPEDIISPRTMEFIDAERTEQLRDQAVKEVKPVYELD
                     TTVEFKAISDIARFFDVLGKTRVKNFAELKLSLPVRLKDDTVKKLFSLGPDEIYKLRS
                     ALIQTVRSILNKGISPQSLNIVDQLIQNEVSGMDIPQELKDLTITLARYFIRPNLSLN
                     MEET"
     gene            complement(636..>1518)
                     /locus_tag="P5310_08355"
     CDS             complement(636..>1518)
                     /locus_tag="P5310_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013432557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HRU33406.1"
                     /translation="RSTNTEIYPVDQGLCIKGNDEDVEKTYAIVEDLSRIAEKGYPVG
                     RKDIGLLLESPERESINISSLRDDVILVTPRGKRIFPKTPGQKKYVDTMRNYDLVFAI
                     GPAGTGKTYLAMAMALVALKNKSVARIILTRPAIEAGERLGFLPGDLQEKVDPYLRPL
                     YDALYDMLETEKIQKYMERGIIEVAPLAYMRGRTLNDAFVILDEAQNTTPEQMKMFLT
                     RMGFGSKYVVTGDITQIDLPLDKESGLIQAQRIFKEVEGLAFVYLTEQDVVRHDLVQR
                     IIRAYNLYESNNKTGRR"
BASE COUNT          425 a          357 c          264 g          472 t
ORIGIN      
        1 tcgtttcttc catatttaaa gagagattgg gtcttataaa gtatcgtgcc agagtaattg
       61 taaggtcttt caactcctga ggtatatcca ttccagagac ctcattctgt atgagctgat
      121 ccactatatt cagactttga ggagatatac ctttattcag aatacttctt actgtctgta
      181 tgagagcact tctaagttta tatatctcat caggtcccag ggaaaaaagc tttttaacag
      241 tatcatcttt taaccttaca ggtaaggata gctttaactc tgcaaaattc ttaactcggg
      301 tttttcccaa tacatcaaaa aaccgcgcta tatcacttat agctttgaat tcaacggtgg
      361 tatcaagctc atagacaggt tttacctctt tgaccgcctg gtcccggagt tgttctgtcc
      421 tctctgcatc tataaactcc atcgtccttg gagagatgat atcctctgga gccacctgtc
      481 ctacagctac ctctatcgaa gagggtaaaa aatcaaatac gaggattccg aaatagaaga
      541 ggaaaactgt aatccagacc agttctctgc ttcttataac cctagggaaa aactggataa
      601 tgaaagacca gatggtcttt atataggaga ataaattatc tcctacccgt tttattattg
      661 gattcataga gattataagc ccttataatt cgctgaacca ggtcatgtct caccacgtcc
      721 tgttcagtca ggtagacaaa agctaagcct tcgacctcct tgaatattct ctgagcctgt
      781 ataagaccag attctttatc taagggcaga tctatctgag ttatgtcacc agtaaccaca
      841 tatttagaac caaaccccat cctggtaagg aacattttca tctgttcagg cgttgtgttc
      901 tgagcctcat caagtataac aaaggcatca tttaatgtcc tgcctctcat atacgctaac
      961 ggagcaacct ctatgatacc tctctccata tatttctgaa tcttctctgt ctccagcata
     1021 tcgtagaggg catcatagag aggtctcaga tatgggtcta ctttctcctg taaatctcca
     1081 ggtaagaaac ctaatctttc acctgcttct attgcaggac gcgtaagaat aatccttgcc
     1141 acggatttat tcttaagagc tacaagagcc attgccatgg caagatatgt cttaccggtt
     1201 ccggctggac caatggcaaa tacgaggtca taattcctca ttgtgtcaac atacttcttc
     1261 tgaccaggtg tcttaggaaa aattctcttc cctctggggg ttacaaggat cacatcatct
     1321 ctaagagaac tgatatttat tgactccctc tccggagact ctaatagaag acctatatct
     1381 ttcctaccta caggataacc cttctccgct attctggaga ggtcttccac tatggcatac
     1441 gtcttttcca catcctcatc attgcccttt atacacaatc cctgatctac aggatatatc
     1501 tcagtattcg tacttctg
//
LOCUS       DAOTKU010000417         1515 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_67528_length_1515_cov_2.556164, whole genome shotgun sequence.
ACCESSION   DAOTKU010000417 DAOTKU010000000
VERSION     DAOTKU010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67528_length_1515_cov_2.556164"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="P5310_08360"
     CDS             <1..528
                     /locus_tag="P5310_08360"
                     /inference="COORDINATES: protein motif:HMM:NF021506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HRU33407.1"
                     /translation="LPTVMDIYKRLGLSGEQLEMLNKNWQEVMNLIKQALPSMLIVGS
                     IIISSANYWIAHNILKRIKIDIPPISISDLRFPPVTILGFFLSFTLTAIGMNNPKSFS
                     YQLGINLQVIFNVLFLLAGLLVINFWLSRWGLPKFARVILFILIIFQPIFSMIATWLG
                     VFDTIFKLRKTEENI"
     gene            548..1495
                     /locus_tag="P5310_08365"
     CDS             548..1495
                     /locus_tag="P5310_08365"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015327417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HRU33408.1"
                     /translation="MDDQQLKDKKIIVLCGWISREREVSLRSGKKIYSALLRKGYDVT
                     LIDPKMEGWEPVLRGDIVFIALHGKPGEDGTIQGFLETFNVPYTGSGVLASSLAMNKL
                     ISKKMFIFSGIPTPDYLSMDDFESIDDFISKSMEYIGLPAVVKPVSEGSSIGVSIVKD
                     ADSLAPVVKDTVNKFDRVFIEKYIEGREITIGIIELDGKPVALPILELVPRNKFYDYE
                     AKYTAGFTEFIIPARMDKEKYTLAQELALKAHKALGCTGFSRVDLITDSNDNPYVLEV
                     NTIPGMTELSDLPAMAKAYGIDYDDLVEIMLQSALREKV"
BASE COUNT          453 a          265 c          344 g          453 t
ORIGIN      
        1 ctacccacag ttatggacat atacaaaaga cttggattat ccggtgaaca gctagagatg
       61 ctcaataaaa actggcagga ggttatgaat cttattaaac aggctcttcc cagtatgctt
      121 attgtgggga gtattataat ctcatctgct aattactgga ttgcccataa catattgaag
      181 aggataaaga tagacatacc tccaatctct atatctgacc ttcgatttcc tcctgtgaca
      241 atactgggat ttttcctctc ttttacactt accgctatag gtatgaataa ccccaaaagc
      301 ttctcttatc agcttggaat aaatcttcag gttatattca atgttctgtt ccttctggct
      361 ggtctactgg ttataaactt ctggttatcc agatggggac tgccaaaatt tgccagggtg
      421 attctgttca tactgataat ctttcagccc attttttcta tgatagccac ctggcttgga
      481 gtatttgata caatatttaa attgagaaag accgaagaaa atatatagac tgaggagaat
      541 aacagctatg gatgatcaac agttaaagga taagaagatt atcgttctat gtggctggat
      601 ttcaagagag agggaggtct ctttaagatc ggggaaaaag atatactccg cattactgag
      661 aaagggttac gatgttaccc tcattgatcc aaagatggag ggatgggagc ctgttttaag
      721 gggtgatata gtattcatag cccttcatgg taaaccgggt gaagatggta ccattcaggg
      781 ctttcttgag accttcaatg ttccatacac aggttcaggt gtcctggctt cttcgctggc
      841 tatgaataag cttataagta aaaagatgtt catcttttcc gggataccaa cccctgatta
      901 tttgagtatg gatgattttg aaagtataga tgatttcata tcaaaatcta tggagtatat
      961 cggtctacct gcggtggtaa aacctgtctc tgaaggttcc agtatagggg tgagtatcgt
     1021 aaaagatgca gactctttag caccggtggt gaaggatacg gtaaataaat ttgatagggt
     1081 ttttattgaa aaatatatag agggaagaga gataacgata gggattatag agcttgatgg
     1141 aaaacctgtc gccctgccca tcctcgagct tgtccccagg aataagtttt atgattatga
     1201 agccaagtat acagctggat ttactgaatt tataattcct gccaggatgg ataaggagaa
     1261 atatacttta gctcaggaac tggctctgaa ggctcataag gcactgggat gtactggatt
     1321 ttccagggtt gaccttatta cagatagtaa cgataacccc tatgtccttg aggtgaatac
     1381 aatccccggg atgacagaac tgagtgacct gcccgctatg gctaaggctt atgggataga
     1441 ttatgatgac ctggttgaga ttatgttaca gtcagccctt agagaaaagg tataggagga
     1501 aaactatgaa acgtt
//
LOCUS       DAOTKU010000418         1514 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_67573_length_1514_cov_4.169294, whole genome shotgun sequence.
ACCESSION   DAOTKU010000418 DAOTKU010000000
VERSION     DAOTKU010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67573_length_1514_cov_4.169294"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            154..>1514
                     /locus_tag="P5310_08370"
     CDS             154..>1514
                     /locus_tag="P5310_08370"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRU33409.1"
                     /translation="MDVIFALDISQSISPQSKEEGLQFIRDAIVSKREADRVGFVVFG
                     EEAFTQSPPDFNPSLDIISSPSLNGTNIEKAIELSNVLLSQSTRNNKRLVLITDGNET
                     DGSAINSAYSSREQGIRIDVLPVLKESLEEVAIDDLVIPERVKLGEPFTIKVILAGSI
                     SQRVRLDILQDEERVLSQELSVKEGKNLFLFSITSNKSGFHKFKVEILPKRDTRTENN
                     TIEAFTVVEGPAKVLYVYNPEDGYGDVLSAIKLQGIDIEAIYPNRLPGSTVGFSGYSA
                     LILDNIPATMLTLNQMRAIQSFVRDAGGGLLTIGGSDSFGMGSYDGTPLEEALPVDSR
                     VIQPANLANIALVLVIDASGSMSGQEGELSKMELAKESAQLTVDLLKDQDKVGVIAFD
                     HSHQWIVELTSAKDKDGIAKKISKVQPGGGTAMYPPMESAYKALKDERARVKHIIVLS
                     DGQT"
BASE COUNT          446 a          285 c          371 g          412 t
ORIGIN      
        1 ccatattttt tgattcttct ttttctggtt tttatgttca tatggcttca gagaaaaaag
       61 atgcacagga ttaccctgtg gctcagagct atctcaatga tttttcttat acttgcgctg
      121 gctcagccca aatttataaa gagagataac agtatggatg taatattcgc tctggatata
      181 tctcagagta tatctcctca gtctaaagag gagggtctcc agtttatcag agacgctatc
      241 gtctctaaaa gagaagcaga tagagttggt tttgtagttt ttggagaaga ggcgttcact
      301 cagagccctc cggattttaa tccatccctg gatataatat cttctccatc tttaaatggg
      361 accaatatag agaaggcgat agaactctca aatgtacttc tctcccaatc caccaggaat
      421 aataaacgac tggtcctgat cacagatggg aatgaaacag atgggtcagc tataaattcg
      481 gcatacagtt ccagagaaca gggaatcagg atagatgtct taccggtact gaaggaatct
      541 cttgaagagg ttgctataga tgacctggtg ataccagaga gggtaaaact tggagaacca
      601 tttactataa aggtaattct ggcaggttca atctcacaga gggtaagact tgatatatta
      661 caggacgagg aaagagtttt atcccaggag ctctctgtta aagagggtaa gaacctattt
      721 ttattcagta taacatcaaa taaaagtggt tttcataaat ttaaagtgga gatcctcccc
      781 aaaagagata caagaactga gaataataca attgaagcct ttactgtggt ggaaggacca
      841 gctaaggtcc tgtacgtgta taatccagag gatggctatg gtgatgttct gtctgccatt
      901 aaattgcagg ggatagatat tgaggcgata tatccgaatc ggctccccgg cagtacggtg
      961 ggtttctcag gatactctgc cctgattctg gataatattc cggcgactat gctcacactg
     1021 aatcagatga gggctatcca gtctttcgtc agggatgctg gtggtgggtt attgaccata
     1081 gggggatcgg atagctttgg tatgggctct tatgatggaa caccccttga agaggctctg
     1141 cctgtagatt ccagagtcat ccagccagcc aaccttgcca atatagctct ggttctggtt
     1201 atagatgcat ctggaagcat gagcgggcag gaaggagaac tctccaagat ggagcttgcc
     1261 aaggagtctg cccagttgac agtggacctg ttgaaagacc aggataaggt gggagtcata
     1321 gcatttgacc attcacatca gtggattgta gagcttacca gtgcaaagga taaggatggt
     1381 attgccaaaa agatatcgaa ggtccagcca ggtggcggaa ctgctatgta tccacctatg
     1441 gaatctgcat ataaggcatt gaaagacgaa agagctaggg taaaacacat tatagtcctg
     1501 tctgatggac agac
//
LOCUS       DAOTKU010000419         1513 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_67638_length_1513_cov_3.334705, whole genome shotgun sequence.
ACCESSION   DAOTKU010000419 DAOTKU010000000
VERSION     DAOTKU010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67638_length_1513_cov_3.334705"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="P5310_08375"
     CDS             <1..324
                     /locus_tag="P5310_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HRU33410.1"
                     /translation="AGSEYLSTKSEGGKNPVKAAIITGSTYVLAVFFLILPYFLFKSY
                     TVSFTFTLIFALLLVVFFTFYISVAQEIDFKRRFLEMFFIIISVSAITFIIGLLVRIF
                     LNVEV"
     gene            341..>1513
                     /locus_tag="P5310_08380"
     CDS             341..>1513
                     /locus_tag="P5310_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011916429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HRU33411.1"
                     /translation="MRKDIELLEQELNNPDLDERLFSLRNLKVLIDTGDIPKPSVNEY
                     AVNNHIHTTYSFSPYSPSKSIWMAYKSGLVTAGIMDHDSISGAKEFISAGKIIGMATT
                     IGVECRVDFSSTPLKGRKLNNPDQETVAYISLHGIPHTKIDTDKDYFSKYQKERNKRN
                     KKMVDRLNSMMKAFDINIDFDRDVVPLSKYNEGGTITERHILFALSLKLIDKFGKGEK
                     LVTFLKDTLRINLTRRIEGYLLDPDNEYYAYDLLGALKAELVPRFFIPATTECPPVEE
                     IVKFSKETGSILAYAYLGDITESVTGDKRSEKFEDDYLDLLFEVLKGLDFDAVTYMPS
                     RNTIEQLKRVKALCDRFGLFQISGEDINSPRQSFICEKLKLEEFRNLIDSTWALIGH"
BASE COUNT          513 a          276 c          296 g          428 t
ORIGIN      
        1 gctggatcag aatatctatc tacaaagtca gagggaggta aaaatccagt aaaggcagct
       61 atcataaccg gaagtacata tgtcctggca gtcttctttc taatacttcc ctactttttg
      121 ttcaaaagtt atactgtttc ttttaccttc accttaatct ttgccttact gctcgtcgtt
      181 tttttcacct tttacatctc tgtagcacag gagatagact tcaagaggag attccttgag
      241 atgttcttca tcataatctc agtatctgct atcaccttta taataggact tctggtaaga
      301 atattcttaa atgtggaagt ataaatagga gaagaaagtt atgaggaaag atattgaatt
      361 gttagagcaa gaattgaata accctgattt agatgagagg ttgttcagtt taagaaatct
      421 aaaagtcctg atagatacgg gggacatacc caagccttcc gttaacgaat atgcagtgaa
      481 caatcatata catacaacat actccttctc accctattca ccatcgaaat ctatatggat
      541 ggcatataaa tctgggcttg taactgctgg tataatggac catgattcca tcagtggggc
      601 aaaagaattt atatctgcag gtaaaattat cggaatggca acaactatcg gagtcgaatg
      661 cagggtagat ttctctagta ccccattaaa aggcaggaaa ctgaataatc cagaccagga
      721 aactgttgcc tacatatctc tccatggaat accacataca aagatagata cggataaaga
      781 ttacttctcc aaatatcaga aggagagaaa caaacgtaat aaaaaaatgg tcgatagatt
      841 aaacagtatg atgaaagctt ttgatataaa tatagacttt gatagagatg tggttcctct
      901 atccaaatat aacgaaggag gaaccataac cgagaggcat atcctctttg cactttcact
      961 taaacttata gataaatttg gtaaaggtga aaaactggta actttcttaa aagatactct
     1021 gaggataaat ctcaccagga gaatagaagg atatctatta gacccagata acgagtatta
     1081 tgcctatgac cttcttgggg cactgaaagc agagcttgtc ccaagattct tcattccagc
     1141 cacaaccgaa tgtccccctg tggaagagat agtaaaattt tccaaagaga caggttcaat
     1201 tctggcatat gcatatctgg gagatattac cgagtcagtt accggggata aaaggagcga
     1261 aaagttcgag gatgactatc tggacctcct ctttgaagtt ttaaaaggac tggactttga
     1321 cgcagtaacc tatatgccat ccaggaatac catagaacag cttaaaaggg taaaagctct
     1381 atgcgacagg tttggacttt tccagataag tggagaggat ataaattctc ccagacaatc
     1441 atttatctgt gagaagttaa aactggaaga attcagaaat cttatagatt ctacctgggc
     1501 tcttatagga cat
//
LOCUS       DAOTKU010000420         1513 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_67674_length_1513_cov_2.090535, whole genome shotgun sequence.
ACCESSION   DAOTKU010000420 DAOTKU010000000
VERSION     DAOTKU010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67674_length_1513_cov_2.090535"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="P5310_08385"
     CDS             complement(<1..426)
                     /locus_tag="P5310_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007935601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRU33412.1"
                     /translation="MDYIALYRKWRSQRFSEVVGQDHIVRALSNAIKMGWISHAYLFS
                     GPRGTGKTTVARIFAKSLNCVNGPTVEPCNICDQCVSIQQGNSLDVVELDAASNRGID
                     EIRSLREQTRFVPIGGRYSVFIIDEAHMLTQEAFNALLKT"
     gene            complement(458..725)
                     /gene="ffs"
                     /locus_tag="P5310_08390"
     ncRNA           complement(458..725)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="P5310_08390"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(742..829)
                     /locus_tag="P5310_08395"
     tRNA            complement(742..829)
                     /locus_tag="P5310_08395"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(793..795),aa:Ser,seq:gga)
     gene            complement(877..>1513)
                     /locus_tag="P5310_08400"
     CDS             complement(877..>1513)
                     /locus_tag="P5310_08400"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HRU33413.1"
                     /translation="LLNLASYNEIYDLSKRALLEDLLWGESLGATGIVVHPGSHKGSG
                     VRNGIFRIVMALEEIISKSNTKCKIILENTSGAGAQLGASPEEFKAIIDSISDKTRLG
                     ICIDTCHLFSAGFDIRNTDGIRETFDRWFSLLDTSYLILFHANDSKGKLGSRLDRHTH
                     IGEGEIGEEGFKSLFNFEIFRTRPIIIETPKNPPGSDEKNIYKLRELGSLL"
BASE COUNT          414 a          396 c          265 g          438 t
ORIGIN      
        1 cgtctttaaa agtgcattaa aagcctcttg agtaagcata tgagcttcat ctatgataaa
       61 tacgctgtat cttcccccaa tagggacaaa tcgggtctgc tctctcagac tccttatctc
      121 atctatccct ctattgctag cagcatctaa ttctacaacg tctaaagaat tcccctgttg
      181 gatagaaaca cactggtcgc atatattaca gggctcaacg gtaggaccat tcacacagtt
      241 taaagattta gcaaatattc ttgcgaccgt agtcttacct gttccccttg gacctgagaa
      301 aagatatgca tgagagatcc accccatctt tatagcatta gaaagtgccc tcactatatg
      361 gtcctgtccc acgacctcac tgaatctctg cgatctccac tttctgtata aggctatata
      421 atccatcttt caattcccct tttaagaaat aaaccggctg tgcaccctcc aatcgattaa
      481 aactgacaca ggattttata catagaacag ctccaaccag gtggtcctat atcacccgga
      541 gatgactact taccgttgct tccttccgga cctggcgggg ttcgtaggtc tccgctgtat
      601 agggcccagt cttcatcgct gtcttgtata aaattgcctg caccagctta acctcaagga
      661 ggggattcag tcctgctata gcgggttgca ggttcagggc accgctaact ccccacctag
      721 cacagccggg tactttcaat atggcggaga gggtgggatt cgaacccacg ggacatttct
      781 gcccactcgc tctccaggcg agcgccttag tccactcagc cacctctcca ctcaataata
      841 aatttatcat aaaggggaac attttacaag agcagtctaa agaaggcttc ccagttctct
      901 caacttataa atgttcttct catcagaacc cgggggattt ttaggagtct ctattataat
      961 tggtctagtc ctaaatatct caaagttaaa taagctttta aatccctctt ccccaatctc
     1021 tccctctcct atatgagtat gtctgtctag ccgggaacca agttttcctt tagaatcatt
     1081 tgcatgaaac aaaattaaat aggatgtatc tagaagcgag aaccatctgt caaaggtttc
     1141 tcttatacca tctgtattcc gtatgtcaaa tcctgccgaa aagagatgac aggtatcaat
     1201 gcagattccc aatctggttt tatcacttat actatctatt atagccttaa actcctccgg
     1261 actagcaccc aactgagctc ctgccccaga agtattctct agaataatct tgcactttgt
     1321 attagactta cttattattt cttccagtgc catcacaatc ctgaaaattc cattccttac
     1381 ccctgaaccc ttatgacttc caggatgtac cactatgcca gtagcaccta aggattcacc
     1441 ccagagaagg tcctctaaca gagccctctt ggataaatcg tatatctcat tgtagctcgc
     1501 cagatttaga aga
//
LOCUS       DAOTKU010000421         1511 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_67795_length_1511_cov_2.981456, whole genome shotgun sequence.
ACCESSION   DAOTKU010000421 DAOTKU010000000
VERSION     DAOTKU010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67795_length_1511_cov_2.981456"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="P5310_08405"
     CDS             <1..486
                     /locus_tag="P5310_08405"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HRU33414.1"
                     /translation="THSHVGTLIEASSHFFDDGKDIDEYPLDAFLGRAILLDAEPGII
                     DEKVIEGLIGDIIKEQDIIICRNKRTGSGYPYLTSESARWLADKKIKMLGIDTNFKLG
                     RDVEEGRLIHSILMSREVTLIEFLSNLDLISRREFFFLALPYKVKLDSSWTRAIAIEE
                     K"
     gene            489..>1511
                     /locus_tag="P5310_08410"
     CDS             489..>1511
                     /locus_tag="P5310_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HRU33415.1"
                     /translation="MGNYILSHDLGTTGNKSTIYDREGNLIASSFYPYSTYYPYPNWV
                     EQNPLDYWEAVKISTRELLARSKVEAGDIAVISFSGQMMGCLPVDRDGNPLRNIIIWA
                     DQRGTEEAEFIREKLGEEKVYRVTGHRISPTYSASKILWIKRNEPEIFLKTHKFLLAK
                     DFIIYKLTGKWVTDYSDASGTNLFDLEEEKWSEEIISSLGIPGEKLPEPYSSLEIVGG
                     LVKSSAEELDLPPGIPVVTGGGDGACAACGAGVVREGDAYNYLGASSWIAIASSKPVY
                     DPELRTFTFHHLAPKLFMPTGTMQSAGLSYQWARDNLAIKEKEIARDLGLSPYEVMDL
                     EAEKILP"
BASE COUNT          462 a          258 c          374 g          417 t
ORIGIN      
        1 acacacagtc acgtcggtac gcttattgaa gccagctctc acttttttga tgatggtaag
       61 gatatagatg agtatccact ggatgccttt ttgggtaggg ctatccttct ggatgccgaa
      121 cctggaataa tagatgaaaa agttatagaa ggattgatag gagatatcat aaaggaacag
      181 gatataatta tttgtagaaa taaaaggaca ggttcaggat atccttatct gacatctgaa
      241 tctgccagat ggctggcgga taaaaagata aagatgttgg ggatagatac caattttaag
      301 ctgggtagag atgttgagga ggggagactt atacatagta ttttaatgtc aagagaggtt
      361 acgcttatcg aatttctcag taatcttgat ctgatttcca ggagggagtt tttcttttta
      421 gcccttccat acaaagtaaa actggatagt tcctggaccc gcgctattgc tatagaagag
      481 aagtagacgt gggtaactat atattatccc atgatcttgg gactacaggg aataaatcta
      541 ctatttatga cagggaggga aatcttattg catcttcttt ttatccttat tctacatatt
      601 atccctatcc taactgggta gaacagaatc cactggacta ttgggaagcg gttaagatat
      661 caaccagaga gcttttagcg aggagtaaag tagaagcagg agatatagct gttataagtt
      721 tcagcgggca gatgatggga tgtctccctg tggatagaga tggtaatcct ctaaggaata
      781 taattatctg ggcagatcag agaggcacag aagaagcgga atttataaga gaaaagctag
      841 gggaagagaa ggtatacagg gttacaggac atcgtataag tcccacttat tctgcctcca
      901 agattttatg gataaagagg aatgagccag aaatatttct taaaacccat aaattcctgt
      961 tagccaaaga ttttataatc tataaactta caggtaaatg ggtaacagat tattccgatg
     1021 cctcagggac aaatctattc gaccttgaag aggaaaagtg gtcggaagag ataatatctt
     1081 ccctgggtat ccctggggaa aaattacccg aaccgtacag ttctctggag attgttggag
     1141 gacttgttaa aagttcagct gaggaacttg atctaccccc gggtattcct gtggtgacag
     1201 gtggtggaga tggtgcctgt gctgcctgtg gtgcaggtgt tgtaagagaa ggagatgcgt
     1261 ataactacct cggtgcttct tcctggatag cgatagcatc cagtaaacct gtatatgatc
     1321 ctgaattaag gacatttaca ttccaccatc ttgccccgaa gttatttatg cccacaggga
     1381 ccatgcagtc tgccgggctt tcctatcagt gggcaagaga taaccttgct attaaagaga
     1441 aagagatagc cagagacctg ggactgagcc cttatgaggt gatggattta gaggcagaga
     1501 agatactccc g
//
LOCUS       DAOTKU010000422         1505 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_68180_length_1505_cov_3.556552, whole genome shotgun sequence.
ACCESSION   DAOTKU010000422 DAOTKU010000000
VERSION     DAOTKU010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68180_length_1505_cov_3.556552"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            202..>1505
                     /locus_tag="P5310_08415"
     CDS             202..>1505
                     /locus_tag="P5310_08415"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HRU33416.1"
                     /translation="MFREIRKRDGRIVKFDAEKITNAITKAGMATGEFDRDIAEKLTL
                     KVLSMAQEVIKDETPTVEEIQDVVEEVLLSSPYRKTAKAYILYRDQHAKIREISAKSS
                     IDLVDQYLEKIDWQVNENSNMTFSLQGLNNYISSEISKIYWLNKIYPPEIRSAHQNGD
                     FHIHDLNSLSVYCVGWDLQDLLLEGFKGVPGKAESKPARHFRSALGQIVNFFYTLQGE
                     AAGAQAFSNFDTFLAPFIRFDNLTYREVKQSLQEFIFNTNVSTRTGFQSPFTNITMDL
                     TVPQFMKDQPVIIGGTPQEATYREFQREMDILNRAFLEVMLEGDAKGRVFTFPIPTYN
                     ITRDFDWNNPNIELLWEVTAKYGIPYFSNFINSDMNPEDVRSMCCRLRISNRERKKRG
                     GGLFGANPLTGSIGVVTINMPRLGYLSRDEDEFFERLERLMIL"
BASE COUNT          507 a          273 c          319 g          406 t
ORIGIN      
        1 cccctcctaa aaaattattt tcactcatta tagcattaat agatataaat agatacgtct
       61 tcgatatctt cgccctgagc aatacgtaat gcgttatcca gattaatatt ttgtacactt
      121 tttgtgggaa accaaatata aaatcttcga tgttagagtt aatgttataa ttagaacata
      181 taattttaaa tggaggaaat tatgttcaga gagatcagaa agagggatgg aaggatagtt
      241 aagtttgacg ctgagaagat aacaaatgct attacaaagg caggcatggc aacaggggaa
      301 ttcgacagag atatagcaga aaaactaaca ttaaaagttt taagcatggc tcaagaagta
      361 ataaaagatg agactcccac agtagaagag atccaggatg tggtagaaga ggttcttcta
      421 tcttccccct accgtaagac agcaaaggct tacatactat acagggacca gcatgctaaa
      481 attagagaga tatcagcgaa atccagtata gacctggtcg accagtacct ggaaaagatt
      541 gactggcaag tcaatgaaaa tagtaatatg acattttccc ttcaaggact aaataattat
      601 atatcatctg agataagtaa gatctactgg cttaataaga tatacccacc agaaatcagg
      661 tctgcacacc agaatggaga ctttcatata cacgacctta acagtctatc tgtctattgt
      721 gttggttggg acctccagga tttattgctg gaaggtttta agggtgttcc aggtaaagct
      781 gaaagcaaac cagccagaca cttcagaagt gctctgggac agatagttaa ctttttctat
      841 acgcttcagg gggaggcagc tggagcccag gcattttcaa actttgacac atttcttgcc
      901 ccttttataa gatttgacaa ccttacctac agagaggtaa aacagtccct gcaggaattt
      961 atattcaata ccaatgtctc gacaagaacc ggcttccaga gtccattcac caatataact
     1021 atggacctga ctgtgcctca gtttatgaaa gaccagcctg taataattgg aggaacgcct
     1081 caggaagcta catatagaga attccagaga gagatggata tattaaatag agccttcctg
     1141 gaggttatgc ttgaaggtga cgccaaagga agggtcttca catttcccat acccacctat
     1201 aatattacaa gagactttga ctggaataat ccaaatatag agctcctctg ggaggttacc
     1261 gccaagtatg gaatacccta cttctccaat tttataaact ctgatatgaa tccggaagat
     1321 gtgaggagta tgtgctgcag gctgaggatc agtaatcgtg aacgtaaaaa gcgtggtggc
     1381 ggtctattcg gagccaatcc attaaccggg tctataggag tagtaactat aaatatgcca
     1441 agattaggat atttatcgag ggatgaagat gaattttttg aaagattaga gaggcttatg
     1501 attct
//
LOCUS       DAOTKU010000423         1503 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_68330_length_1503_cov_3.121547, whole genome shotgun sequence.
ACCESSION   DAOTKU010000423 DAOTKU010000000
VERSION     DAOTKU010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68330_length_1503_cov_3.121547"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            117..>1503
                     /locus_tag="P5310_08420"
     CDS             117..>1503
                     /locus_tag="P5310_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HRU33417.1"
                     /translation="MILDRTIFRMYDIRGEVGREVTPEIMSLIGRAYSVYLKDKIKKD
                     NYTVSVGRDIRLHSKELFDGLVDGLISSGINVIDIGVCPTPVLYFSLFNLDVDGGLMI
                     TASHNPPEFNGLKICVGRETIFGDEIQRLYFIAKDIEDGNIKINNVERGALETSDVLS
                     MYKDYLIHQFKDGPLGSVKPGMIRVGVDSGNGCAGLIVPSILKDLGISLIELYSEPDG
                     RFPNHHPDPTLLESLEELIKTVRDNNLDLGIAYDGDVDRIGVIDENGEVVWGDKLTYI
                     FARDILEKNPGAKIISEVKASKTLFDGIERLGGMPVLSAVGHSLIKKKLREESALLAG
                     EMSGHMFFNDRYFGYDDAIYATLRLLEIVAREKLKDPGFVFSSLLADLPEVYTSPEIR
                     VDSPEEKKFEIVENFLPRFKARYPDVAKNIVRVVTIDGLRVEFPDGWCLIRASNTQPA
                     LVLRFEADTGEK"
BASE COUNT          467 a          222 c          382 g          432 t
ORIGIN      
        1 gtaaagagac atttaatcct gggggcaagc tataaacttg cccctttttc ttttcagata
       61 atgctctatg ttatacttaa tataaaagtt gagattcagg aaggagggaa tcatttatga
      121 tactggatag gaccatattt cgtatgtatg atataagggg tgaggttggg agagaagtta
      181 ctccggagat aatgagcctt ataggaaggg cttacagtgt atatttaaag gataagatta
      241 aaaaggataa ttatactgta tcggttggga gagatataag gcttcactct aaagagctgt
      301 ttgatggtct tgtcgatggg cttatctcca gtggaattaa tgtcatagat atcggtgttt
      361 gcccgacacc ggtgctctat ttttctctat tcaatctgga tgttgatgga ggactgatga
      421 ttactgcaag tcataatcct cctgagttta atggattaaa gatatgtgtg ggcagagaga
      481 caatatttgg agatgagata cagagattgt attttatagc gaaagatata gaggatggaa
      541 atataaagat aaataatgta gaaagaggag ctttggagac ctctgatgtt ttatctatgt
      601 ataaggacta tcttatacat caattcaaag atggaccact gggttcagta aaaccgggga
      661 tgataagagt tggtgtggat agtggaaatg gatgtgcagg gcttatcgtc ccatcaatat
      721 taaaagatct tggtatctct ctcatagaac tctattctga gcctgatgga aggtttccaa
      781 accatcatcc agacccaacg ctccttgaat ctctggaaga actgatcaag actgtgagag
      841 ataataacct ggacctgggg attgcctatg acggagatgt agacagaata ggggtaatag
      901 atgagaatgg agaggtggta tggggagata aacttaccta tatatttgcc agagatatat
      961 tggaaaagaa tcccggtgcc aagattataa gcgaagtaaa ggcttccaag actctatttg
     1021 atggaataga gagacttggt ggaatgccag tcctctctgc agtggggcac tctcttataa
     1081 agaagaaatt gagggaagaa tctgccctcc tggctggaga gatgagcgga catatgttct
     1141 ttaatgacag gtactttgga tatgatgatg ccatatatgc aacactgaga ctcttagaga
     1201 ttgtagcaag agaaaaactt aaggaccctg gttttgtttt ttccagctta ctggcggatt
     1261 taccagaagt ttatacctct ccagagataa gagttgactc acctgaggag aagaagtttg
     1321 agatagtgga gaattttctt cccagattta aagccagata tccggatgtg gcaaagaata
     1381 tagtgagggt tgttaccata gatggattaa gggtggaatt tccagatggg tggtgtctta
     1441 taagagccag caacacacag cctgctctgg ttttaagatt tgaggctgat accggagaaa
     1501 agt
//
LOCUS       DAOTKU010000424         1501 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_2965
            NODE_68470_length_1501_cov_2.931535, whole genome shotgun sequence.
ACCESSION   DAOTKU010000424 DAOTKU010000000
VERSION     DAOTKU010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867989
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:33:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,685
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,637
            CDSs (with protein)               :: 1,637
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68470_length_1501_cov_2.931535"
                     /isolate="AMR_MDS_2965"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1501
                     /gene="mrdA"
                     /locus_tag="P5310_08425"
     CDS             <1..>1501
                     /gene="mrdA"
                     /locus_tag="P5310_08425"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HRU33418.1"
                     /translation="LAKNRASFAVYVIPFEGNREESLKILSGIIGIPYAEIKGKLDKA
                     SNSLQPVNILPDASIEIVGKIKELEDKLPGVLVVTDVIRDYPYKNIASHILGYIGEVS
                     SEEMKIFQEDGVRMGDTIGKAGIERGYQVALMGVRGGEQVEVDAMGRPIKVLGTVPPI
                     PGSTLVTTIDLDLQRIATESLKRRGLPGAIVAIDPQNGDILAMVSSPDYDPNSFAKGI
                     SAKEWERLLSPERPLLNRAISAVYPPGSTFKVAVALAALAEDIVNEDSVFYCPGSMVI
                     GNRRFKCWTSHGRIDFLDAIADSCDVSFYTMGIKVGAEKIARYARLLGLGDLTGVDIP
                     GETRGLIPEPSWKKEPWYIGDTANMSIGQGYVQVTPIQMAVLASTVANGGKVYKPRVV
                     SEIVNQDGTKTSQPAVLVRDLSKFKKEIAIIKKGMKMVVDTGTGTSARVNGISVAGKT
                     GTAENFPTKSNPQGKDHAWFIAYAPTDNPKIALSIVVEQGGHGGSTSAPI"
BASE COUNT          465 a          249 c          401 g          386 t
ORIGIN      
        1 ttggcaaaga atagagccag ttttgctgtt tatgttatac cttttgaagg gaacagggag
       61 gaatctctga aaatattaag tgggataatt ggtatcccct atgcggagat aaagggaaaa
      121 ctggataagg cttctaatag tttgcaaccg gtgaatatac ttccagatgc cagtatagag
      181 attgtaggga agataaaaga gttagaggat aaactacctg gagttttagt agtaacagat
      241 gtgatcagag attatcctta taaaaatatc gcctctcata tcctgggata tataggagag
      301 gtttccagtg aagagatgaa gatatttcag gaagatgggg taagaatggg cgatactata
      361 ggcaaggctg gaattgagag ggggtatcag gtagcactta tgggggtaag aggaggggaa
      421 caggtagagg tagatgctat gggaagaccc ataaaggtgc ttggtacggt tcccccaatt
      481 cctggttcga ctcttgtaac gaccatagat ctggacctgc aaagaatagc tacagaatct
      541 ttaaagagaa gggggttacc gggagctata gttgcgatag accctcaaaa tggagatatc
      601 ctggctatgg taagcagtcc agactatgac cctaatagct ttgccaaagg gataagtgca
      661 aaagagtggg agagactcct ctctcctgaa agacctcttc taaatagagc catatctgct
      721 gtttatccac ctggttctac ttttaaggta gctgttgctc tggctgctct ggctgaggat
      781 atagtaaatg aagattcagt attttactgc cctgggtcaa tggttatagg aaatcgtaga
      841 tttaaatgct ggacttctca tggaaggata gactttttgg atgcaatagc tgactcctgc
      901 gatgtgtctt tttatactat ggggattaaa gttggagcag aaaagatagc caggtatgca
      961 agattgcttg gattgggaga tctaactggg gtagatatcc ctggtgaaac aagagggctt
     1021 atacctgaac cttcctggaa aaaggaaccg tggtatatag gagatacagc taatatgtct
     1081 ataggacagg gatatgtcca ggttactcca atacagatgg ctgtccttgc aagtacggta
     1141 gcaaatggcg gcaaggtata taaaccgagg gtcgtctcag agatagtaaa tcaggatggg
     1201 actaagacct cacagccagc ggtgcttgta agagacctgt ctaaatttaa aaaagaaatt
     1261 gctataatta agaagggtat gaagatggtt gtagatactg gtacagggac ttcagccaga
     1321 gttaatggaa taagtgttgc cgggaaaaca ggaacagcag aaaatttccc cactaaaagt
     1381 aatcctcaag gaaaggacca tgcctggttt attgcatacg ccccgacgga taatccgaag
     1441 atagcactat ctatagttgt ggaacagggg ggacacggag ggtccacttc tgctcctatc
     1501 g
//
